Dpse\GA28220 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA28220
Entrez Gene ID	6900273
Full Name	GA28220 gene product from transcript GA28220-RB
Synonyms	GA28220,dpse_GLEANR_7732
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015188566.1	XP_015044052.1	uncharacterized protein, isoform C
XM_002135565.2	XP_002135601.1	uncharacterized protein, isoform A
XM_015188565.1	XP_015044051.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA28220 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA28220 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe27401	XM_015188566.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA28220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODe12365	XM_015188565.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA28220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODe12365	XM_002135565.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA28220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA28220 ( XM_015188566.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015044052.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA28220), mRNA.

Price & Availability↑

CloneID	ODe27401
Clone ID Related Accession (Same CDS sequence)	XM_015188566.1
Accession Version	XM_015188566.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589954). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGGGT TTGCCAACGA ACTGTGCAAA AAGGACGACA CACACTATAC CTCCACAGTG 
GCTGCCGTGG TGCTGGGGGT GCTGCTCTTC TGCTCTGGCG TGATCACCAT GAGCATCTAT 
CGCAAGTGGA AGATTGAGCT GGAGATCGAG GGTTTGCTGT GGAAGATCGA TCCAAACGAA 
ATCAAGGGCT ACTCCGGCAA CGAGATTGTC TCCTCGCCCA GCAAGGTCAG TTTGATGAGC 
GCCCAGAGCT ATGGCTCACG CTGGACAAAT CAGTTTGTGA CCTCCACGGG TCGCCTACGA 
GGCGCAGTGG TGCGCATCAA AGAATTGAAG TTCCCGCGCA AGCGGGACAT CTCCAGGGAG 
ATCATGAAGG AGATGCGTTT GCTGCGCGAG CTGCGACACG ACAATATCAA CAGCTTCATT 
GGCGCCTCTG TGGAGCCGAC GCGCATCCTG CTAGTCACCG ATTACTGCGC CAAGGGCAGT 
CTGTACGATA TCATCGAGAA CGAAGACATC AAATTGGACG ATCTGTTCAT TGCCTCACTC 
ATCCATGACC TCATCAAGGG CATGATCTAC ATCCACAATT CCCAATTGAT CTATCATGGC 
AATCTCAAAT CCTCGAATTG TGTGGTCACC TCGCGCTGGA TGTTGCAAGT CACCGACTTT 
GGGCTGCACG AGCTGCGCCA GTGCGCCGAG AACGAGTCGA TTGGGGAGCA TCAGCATTAC 
CGAAATCAAC TGTGGCGCGC ACCGGAACTG CTGCGGAATC ATATCCATGG CAGCCAGAAG 
GGCGATGTCT ATGCGTTTGC CATCATCATG TACGAGATAT TTAGTCGTAA GGGTCCGTTC 
GGACAAACCA TTTTAGAACC AAAGGATATT GTGGACTTTG TTAAGAAGCT GCCGCTCAAT 
GGCCAGGAGC CGTTTCGGCC GGAGGTCGAG TCCATAATAG AGGCCGAATC CTGTCCGGAT 
TATGTGCTGG CCTGCATCAA GGACTGCTGG GCCGAGGAGC CCGAAGAGCG GCCCGAATTC 
AGTGCCATAC GAAATCGCTT GAAGAAGATG CGCGGCGGTA AAACCAAAAA CATCATGGAC 
CAAATGATGG AAATGATGGA GAAGTATGCC AACAACCTGG AGGACATTGT CACCGAGCGC 
ACACGTCTGC TGTGCGAGGA GAAGCTCAAG ACAGAGGATC TGCTGCATCG CATGTTGCCC 
CAGTCGGTGG CCGAGAAGCT GACCATGGGT CAGGGCGTTG AGCCGGTGTC CTATGATTTG 
GTAACGATCT ATTTCAGCGA CATTGTCGGC TTCACGGCCA TGTCAGCGGA GAGCACACCG 
CTGCAGGTGG TTAACTTTCT GAATGATCTC TACACGGTGT TCGATCGCAT CATTCGCGGC 
TATGATGTGT ACAAGGTGGA GACCATAGGC GATGCCTACA TGGTGGTCTC TGGTCTCCCC 
ATTAAGAATG GGGATCGTCA TGCGGGCGAG ATAGCTTCGA TGGCTCTGGA GCTGCTGCAG 
GCGGTGAAAC AGCATCGCAT CGCCCATCGT CCGAACGAGA CGCTGAAGCT GAGAATCGGA 
ATGCATACGG GCCCCGTGGT GGCCGGCGTC GTGGGCCTCA CGATGCCGAG GTACTGCCTC 
TTTGGCGACA CCGTGAACAC GGCCTCACGA ATGGAGAGCA ATGGCGAGGC GCTGAAGATA 
CACATTTCCA ACAAGTGTAA GCTGGCGCTG GACAAACTCG GAGGCGGCTA CATCACCGAG 
AAGCGTGGCC TGGTCAATAT GAAGGGAAAG GGCGATGTGA TCACCTGGTG GCTGACGGGG 
GCCAACGATA TTGCGATACA GAAGAAGCCT GTGGACATGA TGGACATGCC GCCCCTGTTC 
TCCCGTCCGA GGAAGAGTCC GAAGCTGAAC TCCGACTCCA GGCAGCCAAG TATTCAGGCC 
ATGCACTTCT GCGGGACAGG ATCGAGGCGT CAGAGCAGTG TGCCAAGGCC GGCGGATGTC 
GAGTCCACGT ACAGCCTGCA GGGGTCCGTG AGGGAGTCGC CGCGAGTCAG CAAGAGGGAC 
AGGGACAGGG AGCGACCCGT CAATGGACTG GGTGGAGCAC ACTTTGTGGG CGGAGCACTC 
CTGGAGTCGG CTCTGGCCTC GCTCAGCACG CTGAATCATT CCGAAACAAA TGAAACGAAC 
TGTGATATGG ATGGAGGAGT AGGAGGAGCG AGTCTAGTAC GCCAGCCAAA CGCTCTGCAC 
AAGCCCCTGG CCATGGTGCG GCCCCACCGG ATCATCAGTG CCGCCCAGCT GCCCCTCGGG 
GAGGACGACG ACGCCGACGG CACCACCGTC TCCCAGATGG ACGTGGAGAT GCTCCTGAGG 
GAATCTCGCT CCCTGGATCC CATGCCGTGG CAGCATCTGC GCAAGCGGCA CGAGAGGGTC 
AAGCTGCCAC CCTCCAAGCT GTCCAAAAAC AATTCGCGAT CCCTGGACAC GGGAGTGTCG 
CTGATCAGCG GCAACCAGAA CGGCGATGCC GAGGGGAGCC AACTGGAGAT CGACGACGAC 
ATGTCCGCCA ACCCAGTGGA TGCCAACGAT AGCTATGACG ATGAGCTGGG CCTGCTCATG 
CGACACGACA ACGGCCAGCT GCCCACGCTC CGCTACTCCG GCAGCTTCCC CAATGCCAAT 
GCCCACGCCC ATGGCCAGAT CAGCATCGTT CCCACCGGCG GCAGCTCCAG CCGACGGCGC 
ACCAGCAATG TCTCCAGCGG CAGCTGTGCC AAGCATCTGA ACAACAACTG CAACGGCGGC 
ATGAATATCG AGGACGATCT GGAGTCTCCC CTGCTGCAGC GCCAGGCCTC GCTCTCTGTG 
CCCCCCGAGG AAATGTTGGC GCACAACAAG CGCTGGCACT CGCTAGAGCA CATGGAGCCG 
GGCGGTGCCG GGCACGGGAA CAGTGTCAGC TATGCGGCGG ACATCGATGG CCCTGTCAGT 
GGCTTGGACA TCTTGGGCGG CTCCTCCTCC AATCAGCGTC CCAGAGGTGT CGGTGGCACA 
GGCGGCGGAG CCACCAAGCT GGGCAACTGG ATGGCCAATT TCTTCAAGGG CAATGGAGTG 
CGCAGCGGGG AGGCTAGGCG AGTGGGAATC CTGCCCACCG GTGTGCAAGG AGTAAGAGCT 
GGATTCACGG ATATGACGGC AGCGGCAGCA GCCGGAGCCC GAGATCGTGA GAGTATAGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015044052.1
CDS	160..3342
Translation	MCGFANELCKKDDTHYTSTVAAVVLGVLLFCSGVITMSIYRKWKIELEIEGLLWKIDPNEIKGYSGNEIVSSPSKVSLMSAQSYGSRWTNQFVTSTGRLRGAVVRIKELKFPRKRDISREIMKEMRLLRELRHDNINSFIGASVEPTRILLVTDYCAKGSLYDIIENEDIKLDDLFIASLIHDLIKGMIYIHNSQLIYHGNLKSSNCVVTSRWMLQVTDFGLHELRQCAENESIGEHQHYRNQLWRAPELLRNHIHGSQKGDVYAFAIIMYEIFSRKGPFGQTILEPKDIVDFVKKLPLNGQEPFRPEVESIIEAESCPDYVLACIKDCWAEEPEERPEFSAIRNRLKKMRGGKTKNIMDQMMEMMEKYANNLEDIVTERTRLLCEEKLKTEDLLHRMLPQSVAEKLTMGQGVEPVSYDLVTIYFSDIVGFTAMSAESTPLQVVNFLNDLYTVFDRIIRGYDVYKVETIGDAYMVVSGLPIKNGDRHAGEIASMALELLQAVKQHRIAHRPNETLKLRIGMHTGPVVAGVVGLTMPRYCLFGDTVNTASRMESNGEALKIHISNKCKLALDKLGGGYITEKRGLVNMKGKGDVITWWLTGANDIAIQKKPVDMMDMPPLFSRPRKSPKLNSDSRQPSIQAMHFCGTGSRRQSSVPRPADVESTYSLQGSVRESPRVSKRDRDRERPVNGLGGAHFVGGALLESALASLSTLNHSETNETNCDMDGGVGGASLVRQPNALHKPLAMVRPHRIISAAQLPLGEDDDADGTTVSQMDVEMLLRESRSLDPMPWQHLRKRHERVKLPPSKLSKNNSRSLDTGVSLISGNQNGDAEGSQLEIDDDMSANPVDANDSYDDELGLLMRHDNGQLPTLRYSGSFPNANAHAHGQISIVPTGGSSSRRRTSNVSSGSCAKHLNNNCNGGMNIEDDLESPLLQRQASLSVPPEEMLAHNKRWHSLEHMEPGGAGHGNSVSYAADIDGPVSGLDILGGSSSNQRPRGVGGTGGGATKLGNWMANFFKGNGVRSGEARRVGILPTGVQGVRAGFTDMTAAAAAGARDRESIV

Target ORF information:

RefSeq Version	XM_015188566.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA28220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015188566.1

ORF Insert Sequence:

ATGTGCGGGT TTGCCAACGA ACTGTGCAAA AAGGACGACA CACACTATAC CTCCACAGTG 
GCTGCCGTGG TGCTGGGGGT GCTGCTCTTC TGCTCTGGCG TGATCACCAT GAGCATCTAT 
CGCAAGTGGA AGATTGAGCT GGAGATCGAG GGTTTGCTGT GGAAGATCGA TCCAAACGAA 
ATCAAGGGCT ACTCCGGCAA CGAGATTGTC TCCTCGCCCA GCAAGGTCAG TTTGATGAGC 
GCCCAGAGCT ATGGCTCACG CTGGACAAAT CAGTTTGTGA CCTCCACGGG TCGCCTACGA 
GGCGCAGTGG TGCGCATCAA AGAATTGAAG TTCCCGCGCA AGCGGGACAT CTCCAGGGAG 
ATCATGAAGG AGATGCGTTT GCTGCGCGAG CTGCGACACG ACAATATCAA CAGCTTCATT 
GGCGCCTCTG TGGAGCCGAC GCGCATCCTG CTAGTCACCG ATTACTGCGC CAAGGGCAGT 
CTGTACGATA TCATCGAGAA CGAAGACATC AAATTGGACG ATCTGTTCAT TGCCTCACTC 
ATCCATGACC TCATCAAGGG CATGATCTAC ATCCACAATT CCCAATTGAT CTATCATGGC 
AATCTCAAAT CCTCGAATTG TGTGGTCACC TCGCGCTGGA TGTTGCAAGT CACCGACTTT 
GGGCTGCACG AGCTGCGCCA GTGCGCCGAG AACGAGTCGA TTGGGGAGCA TCAGCATTAC 
CGAAATCAAC TGTGGCGCGC ACCGGAACTG CTGCGGAATC ATATCCATGG CAGCCAGAAG 
GGCGATGTCT ATGCGTTTGC CATCATCATG TACGAGATAT TTAGTCGTAA GGGTCCGTTC 
GGACAAACCA TTTTAGAACC AAAGGATATT GTGGACTTTG TTAAGAAGCT GCCGCTCAAT 
GGCCAGGAGC CGTTTCGGCC GGAGGTCGAG TCCATAATAG AGGCCGAATC CTGTCCGGAT 
TATGTGCTGG CCTGCATCAA GGACTGCTGG GCCGAGGAGC CCGAAGAGCG GCCCGAATTC 
AGTGCCATAC GAAATCGCTT GAAGAAGATG CGCGGCGGTA AAACCAAAAA CATCATGGAC 
CAAATGATGG AAATGATGGA GAAGTATGCC AACAACCTGG AGGACATTGT CACCGAGCGC 
ACACGTCTGC TGTGCGAGGA GAAGCTCAAG ACAGAGGATC TGCTGCATCG CATGTTGCCC 
CAGTCGGTGG CCGAGAAGCT GACCATGGGT CAGGGCGTTG AGCCGGTGTC CTATGATTTG 
GTAACGATCT ATTTCAGCGA CATTGTCGGC TTCACGGCCA TGTCAGCGGA GAGCACACCG 
CTGCAGGTGG TTAACTTTCT GAATGATCTC TACACGGTGT TCGATCGCAT CATTCGCGGC 
TATGATGTGT ACAAGGTGGA GACCATAGGC GATGCCTACA TGGTGGTCTC TGGTCTCCCC 
ATTAAGAATG GGGATCGTCA TGCGGGCGAG ATAGCTTCGA TGGCTCTGGA GCTGCTGCAG 
GCGGTGAAAC AGCATCGCAT CGCCCATCGT CCGAACGAGA CGCTGAAGCT GAGAATCGGA 
ATGCATACGG GCCCCGTGGT GGCCGGCGTC GTGGGCCTCA CGATGCCGAG GTACTGCCTC 
TTTGGCGACA CCGTGAACAC GGCCTCACGA ATGGAGAGCA ATGGCGAGGC GCTGAAGATA 
CACATTTCCA ACAAGTGTAA GCTGGCGCTG GACAAACTCG GAGGCGGCTA CATCACCGAG 
AAGCGTGGCC TGGTCAATAT GAAGGGAAAG GGCGATGTGA TCACCTGGTG GCTGACGGGG 
GCCAACGATA TTGCGATACA GAAGAAGCCT GTGGACATGA TGGACATGCC GCCCCTGTTC 
TCCCGTCCGA GGAAGAGTCC GAAGCTGAAC TCCGACTCCA GGCAGCCAAG TATTCAGGCC 
ATGCACTTCT GCGGGACAGG ATCGAGGCGT CAGAGCAGTG TGCCAAGGCC GGCGGATGTC 
GAGTCCACGT ACAGCCTGCA GGGGTCCGTG AGGGAGTCGC CGCGAGTCAG CAAGAGGGAC 
AGGGACAGGG AGCGACCCGT CAATGGACTG GGTGGAGCAC ACTTTGTGGG CGGAGCACTC 
CTGGAGTCGG CTCTGGCCTC GCTCAGCACG CTGAATCATT CCGAAACAAA TGAAACGAAC 
TGTGATATGG ATGGAGGAGT AGGAGGAGCG AGTCTAGTAC GCCAGCCAAA CGCTCTGCAC 
AAGCCCCTGG CCATGGTGCG GCCCCACCGG ATCATCAGTG CCGCCCAGCT GCCCCTCGGG 
GAGGACGACG ACGCCGACGG CACCACCGTC TCCCAGATGG ACGTGGAGAT GCTCCTGAGG 
GAATCTCGCT CCCTGGATCC CATGCCGTGG CAGCATCTGC GCAAGCGGCA CGAGAGGGTC 
AAGCTGCCAC CCTCCAAGCT GTCCAAAAAC AATTCGCGAT CCCTGGACAC GGGAGTGTCG 
CTGATCAGCG GCAACCAGAA CGGCGATGCC GAGGGGAGCC AACTGGAGAT CGACGACGAC 
ATGTCCGCCA ACCCAGTGGA TGCCAACGAT AGCTATGACG ATGAGCTGGG CCTGCTCATG 
CGACACGACA ACGGCCAGCT GCCCACGCTC CGCTACTCCG GCAGCTTCCC CAATGCCAAT 
GCCCACGCCC ATGGCCAGAT CAGCATCGTT CCCACCGGCG GCAGCTCCAG CCGACGGCGC 
ACCAGCAATG TCTCCAGCGG CAGCTGTGCC AAGCATCTGA ACAACAACTG CAACGGCGGC 
ATGAATATCG AGGACGATCT GGAGTCTCCC CTGCTGCAGC GCCAGGCCTC GCTCTCTGTG 
CCCCCCGAGG AAATGTTGGC GCACAACAAG CGCTGGCACT CGCTAGAGCA CATGGAGCCG 
GGCGGTGCCG GGCACGGGAA CAGTGTCAGC TATGCGGCGG ACATCGATGG CCCTGTCAGT 
GGCTTGGACA TCTTGGGCGG CTCCTCCTCC AATCAGCGTC CCAGAGGTGT CGGTGGCACA 
GGCGGCGGAG CCACCAAGCT GGGCAACTGG ATGGCCAATT TCTTCAAGGG CAATGGAGTG 
CGCAGCGGGG AGGCTAGGCG AGTGGGAATC CTGCCCACCG GTGTGCAAGG AGTAAGAGCT 
GGATTCACGG ATATGACGGC AGCGGCAGCA GCCGGAGCCC GAGATCGTGA GAGTATAGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA28220 ( XM_002135565.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002135601.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA28220), mRNA.

Price & Availability↑

CloneID	ODe12365
Clone ID Related Accession (Same CDS sequence)	XM_002135565.2 , XM_015188565.1
Accession Version	XM_002135565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589954). On Dec 22, 2015 this sequence version replaced XM_002135565.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCAGTGCG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC AGTTGGCTAT CTGACGGCTC TCACTGGCGA GCTCAAGACG 
CGTCAGGGCT TGGCCATATC CGGTGCGCTG ACGATGGCCC TGGATGAGGT CAACAAGGAT 
CCGAATCTGC TGCCAAATGT GACGCTCGAC CTGCGCTGGA ATGACACCAA GGGTGATACG 
GTATTGGCCA CCAAAGCCAT TACCGAAATG ATATGCGATG GCATTGCCAC CATATTCGGG 
CCGGAGGGTC CTTGTTATGT GGAGGCCATC GTGTCGCAGA GTCGCAACAT TCCAATGATA 
TCCTATAAAT GCGCAGAGTA CCGTGCATCC GCCATACCGA CGTTCGCCCG CACGGAACCG 
CCAGACACGC AGGTCGTCAA ATCGCTGCTC GCCTTGTTGC GCTACTATGC CTGGAACAAG 
TTCTCCATCC TGTACGAGGA CGTGTGGGGC CCGGTGGCGG ACTTGCTCAA GGATCAGGCC 
ACCAAGCGGA ACATGACGAT CAACCACAAG CAGTCGTTCA TCGACAATCG GGCCAAATGC 
TGCGAACTGG GGCTCGATTG CTGTCGCTCC GGCTATTGGT ATCAGCTGGT CCAGAATACG 
ATGAACCGCA CGAGGATCTA CGTGTTTCTG GGTGCCGCCA ACAGCCTGGT GGACTTTATG 
AGCTCCATGG AGACGGCCGG ACTGTTTGCG CGCGGCGAAT ACATGGTGAT CTTTGTGGAC 
ATGATGGTGT ACTCGGAACG TGAGGCAGAG AAGTACTTGC GGAAAGTGGA TCAGATCGAT 
CTCATGTCCA ACTGCCACAG CACCGAGAAG TTCAACCAGC TGGCACGCAG TCTGCTCGTC 
GTGGCCTCCA CGCCGCCGAC CAAGGGCTAT GAGAAGTTCA CAGAACGCGT GCGGACCTAC 
AGCTCGATGC CGCCGTTCAA CTTTGAGATA CCCAAACTGT TTTCCCAAAG TAAATTTAGC 
AAGTTCATAT CGATCTATGC CGCCTATCTG TACGATTCGG TGAAACTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGCCA GGAGACGCGT GTGCTCACCG ACGAGGTGAT CTTCGATGTG 
GCCAGCAATG GGACGCGTGT CATCGATACC ATCATCAAGA ATCGTACTTA TATGAGCGTC 
ACTGGGTCCA CGATCAAGAT CGATCGTTAT GGCGACTCGG AGGGGAACTT CTCAGTGCTG 
GCCTACAAGC CCCACAAATG GAACAACTCG AACGGAGTGA TATGCCACTA CCACATGGTG 
CCCGTGGCGT ATTTCCATCA AGGCGAAGAA CTTCCAGAAT ACAAACTGAT CAACGGCTCT 
ATAGACTGGC CCTCGGGCGG CGAGAAGCCC ACGGATGAGC CCATGTGCGG GTTTGCCAAC 
GAACTGTGCA AAAAGGACGA CACACACTAT ACCTCCACAG TGGCTGCCGT GGTGCTGGGG 
GTGCTGCTCT TCTGCTCTGG CGTGATCACC ATGAGCATCT ATCGCAAGTG GAAGATTGAG 
CTGGAGATCG AGGGTTTGCT GTGGAAGATC GATCCAAACG AAATCAAGGG CTACTCCGGC 
AACGAGATTG TCTCCTCGCC CAGCAAGGTC AGTTTGATGA GCGCCCAGAG CTATGGCTCA 
CGCTGGACAA ATCAGTTTGT GACCTCCACG GGTCGCCTAC GAGGCGCAGT GGTGCGCATC 
AAAGAATTGA AGTTCCCGCG CAAGCGGGAC ATCTCCAGGG AGATCATGAA GGAGATGCGT 
TTGCTGCGCG AGCTGCGACA CGACAATATC AACAGCTTCA TTGGCGCCTC TGTGGAGCCG 
ACGCGCATCC TGCTAGTCAC CGATTACTGC GCCAAGGGCA GTCTGTACGA TATCATCGAG 
AACGAAGACA TCAAATTGGA CGATCTGTTC ATTGCCTCAC TCATCCATGA CCTCATCAAG 
GGCATGATCT ACATCCACAA TTCCCAATTG ATCTATCATG GCAATCTCAA ATCCTCGAAT 
TGTGTGGTCA CCTCGCGCTG GATGTTGCAA GTCACCGACT TTGGGCTGCA CGAGCTGCGC 
CAGTGCGCCG AGAACGAGTC GATTGGGGAG CATCAGCATT ACCGAAATCA ACTGTGGCGC 
GCACCGGAAC TGCTGCGGAA TCATATCCAT GGCAGCCAGA AGGGCGATGT CTATGCGTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAC CATTTTAGAA 
CCAAAGGATA TTGTGGACTT TGTTAAGAAG CTGCCGCTCA ATGGCCAGGA GCCGTTTCGG 
CCGGAGGTCG AGTCCATAAT AGAGGCCGAA TCCTGTCCGG ATTATGTGCT GGCCTGCATC 
AAGGACTGCT GGGCCGAGGA GCCCGAAGAG CGGCCCGAAT TCAGTGCCAT ACGAAATCGC 
TTGAAGAAGA TGCGCGGCGG TAAAACCAAA AACATCATGG ACCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACCT GGAGGACATT GTCACCGAGC GCACACGTCT GCTGTGCGAG 
GAGAAGCTCA AGACAGAGGA TCTGCTGCAT CGCATGTTGC CCCAGTCGGT GGCCGAGAAG 
CTGACCATGG GTCAGGGCGT TGAGCCGGTG TCCTATGATT TGGTAACGAT CTATTTCAGC 
GACATTGTCG GCTTCACGGC CATGTCAGCG GAGAGCACAC CGCTGCAGGT GGTTAACTTT 
CTGAATGATC TCTACACGGT GTTCGATCGC ATCATTCGCG GCTATGATGT GTACAAGGTG 
GAGACCATAG GCGATGCCTA CATGGTGGTC TCTGGTCTCC CCATTAAGAA TGGGGATCGT 
CATGCGGGCG AGATAGCTTC GATGGCTCTG GAGCTGCTGC AGGCGGTGAA ACAGCATCGC 
ATCGCCCATC GTCCGAACGA GACGCTGAAG CTGAGAATCG GAATGCATAC GGGCCCCGTG 
GTGGCCGGCG TCGTGGGCCT CACGATGCCG AGGTACTGCC TCTTTGGCGA CACCGTGAAC 
ACGGCCTCAC GAATGGAGAG CAATGGCGAG GCGCTGAAGA TACACATTTC CAACAAGTGT 
AAGCTGGCGC TGGACAAACT CGGAGGCGGC TACATCACCG AGAAGCGTGG CCTGGTCAAT 
ATGAAGGGAA AGGGCGATGT GATCACCTGG TGGCTGACGG GGGCCAACGA TATTGCGATA 
CAGAAGAAGC CTGTGGACAT GATGGACATG CCGCCCCTGT TCTCCCGTCC GAGGAAGAGT 
CCGAAGCTGA ACTCCGACTC CAGGCAGCCA AGTATTCAGG CCATGCACTT CTGCGGGACA 
GGATCGAGGC GTCAGAGCAG TGTGCCAAGG CCGGCGGATG TCGAGTCCAC GTACAGCCTG 
CAGGGGTCCG TGAGGGAGTC GCCGCGAGTC AGCAAGAGGG ACAGGGACAG GGAGCGACCC 
GTCAATGGAC TGGGTGGAGC ACACTTTGTG GGCGGAGCAC TCCTGGAGTC GGCTCTGGCC 
TCGCTCAGCA CGCTGAATCA TTCCGAAACA AATGAAACGA ACTGTGATAT GGATGGAGGA 
GTAGGAGGAG CGAGTCTAGT ACGCCAGCCA AACGCTCTGC ACAAGCCCCT GGCCATGGTG 
CGGCCCCACC GGATCATCAG TGCCGCCCAG CTGCCCCTCG GGGAGGACGA CGACGCCGAC 
GGCACCACCG TCTCCCAGAT GGACGTGGAG ATGCTCCTGA GGGAATCTCG CTCCCTGGAT 
CCCATGCCGT GGCAGCATCT GCGCAAGCGG CACGAGAGGG TCAAGCTGCC ACCCTCCAAG 
CTGTCCAAAA ACAATTCGCG ATCCCTGGAC ACGGGAGTGT CGCTGATCAG CGGCAACCAG 
AACGGCGATG CCGAGGGGAG CCAACTGGAG ATCGACGACG ACATGTCCGC CAACCCAGTG 
GATGCCAACG ATAGCTATGA CGATGAGCTG GGCCTGCTCA TGCGACACGA CAACGGCCAG 
CTGCCCACGC TCCGCTACTC CGGCAGCTTC CCCAATGCCA ATGCCCACGC CCATGGCCAG 
ATCAGCATCG TTCCCACCGG CGGCAGCTCC AGCCGACGGC GCACCAGCAA TGTCTCCAGC 
GGCAGCTGTG CCAAGCATCT GAACAACAAC TGCAACGGCG GCATGAATAT CGAGGACGAT 
CTGGAGTCTC CCCTGCTGCA GCGCCAGGCC TCGCTCTCTG TGCCCCCCGA GGAAATGTTG 
GCGCACAACA AGCGCTGGCA CTCGCTAGAG CACATGGAGC CGGGCGGTGC CGGGCACGGG 
AACAGTGTCA GCTATGCGGC GGACATCGAT GGCCCTGTCA GTGGCTTGGA CATCTTGGGC 
GGCTCCTCCT CCAATCAGCG TCCCAGAGGT GTCGGTGGCA CAGGCGGCGG AGCCACCAAG 
CTGGGCAACT GGATGGCCAA TTTCTTCAAG GGCAATGGAG TGCGCAGCGG GGAGGCTAGG 
CGAGTGGGAA TCCTGCCCAC CGGTGTGCAA GGAGTAAGAG CTGGATTCAC GGATATGACG 
GCAGCGGCAG CAGCCGGAGC CCGAGATCGT GAGAGTATAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002135601.1
CDS	552..5156
Translation	MTRWPFNLLLLLSVAVRDCSNHRTVLTVGYLTALTGELKTRQGLAISGALTMALDEVNKDPNLLPNVTLDLRWNDTKGDTVLATKAITEMICDGIATIFGPEGPCYVEAIVSQSRNIPMISYKCAEYRASAIPTFARTEPPDTQVVKSLLALLRYYAWNKFSILYEDVWGPVADLLKDQATKRNMTINHKQSFIDNRAKCCELGLDCCRSGYWYQLVQNTMNRTRIYVFLGAANSLVDFMSSMETAGLFARGEYMVIFVDMMVYSEREAEKYLRKVDQIDLMSNCHSTEKFNQLARSLLVVASTPPTKGYEKFTERVRTYSSMPPFNFEIPKLFSQSKFSKFISIYAAYLYDSVKLYAWAVDKMLRQETRVLTDEVIFDVASNGTRVIDTIIKNRTYMSVTGSTIKIDRYGDSEGNFSVLAYKPHKWNNSNGVICHYHMVPVAYFHQGEELPEYKLINGSIDWPSGGEKPTDEPMCGFANELCKKDDTHYTSTVAAVVLGVLLFCSGVITMSIYRKWKIELEIEGLLWKIDPNEIKGYSGNEIVSSPSKVSLMSAQSYGSRWTNQFVTSTGRLRGAVVRIKELKFPRKRDISREIMKEMRLLRELRHDNINSFIGASVEPTRILLVTDYCAKGSLYDIIENEDIKLDDLFIASLIHDLIKGMIYIHNSQLIYHGNLKSSNCVVTSRWMLQVTDFGLHELRQCAENESIGEHQHYRNQLWRAPELLRNHIHGSQKGDVYAFAIIMYEIFSRKGPFGQTILEPKDIVDFVKKLPLNGQEPFRPEVESIIEAESCPDYVLACIKDCWAEEPEERPEFSAIRNRLKKMRGGKTKNIMDQMMEMMEKYANNLEDIVTERTRLLCEEKLKTEDLLHRMLPQSVAEKLTMGQGVEPVSYDLVTIYFSDIVGFTAMSAESTPLQVVNFLNDLYTVFDRIIRGYDVYKVETIGDAYMVVSGLPIKNGDRHAGEIASMALELLQAVKQHRIAHRPNETLKLRIGMHTGPVVAGVVGLTMPRYCLFGDTVNTASRMESNGEALKIHISNKCKLALDKLGGGYITEKRGLVNMKGKGDVITWWLTGANDIAIQKKPVDMMDMPPLFSRPRKSPKLNSDSRQPSIQAMHFCGTGSRRQSSVPRPADVESTYSLQGSVRESPRVSKRDRDRERPVNGLGGAHFVGGALLESALASLSTLNHSETNETNCDMDGGVGGASLVRQPNALHKPLAMVRPHRIISAAQLPLGEDDDADGTTVSQMDVEMLLRESRSLDPMPWQHLRKRHERVKLPPSKLSKNNSRSLDTGVSLISGNQNGDAEGSQLEIDDDMSANPVDANDSYDDELGLLMRHDNGQLPTLRYSGSFPNANAHAHGQISIVPTGGSSSRRRTSNVSSGSCAKHLNNNCNGGMNIEDDLESPLLQRQASLSVPPEEMLAHNKRWHSLEHMEPGGAGHGNSVSYAADIDGPVSGLDILGGSSSNQRPRGVGGTGGGATKLGNWMANFFKGNGVRSGEARRVGILPTGVQGVRAGFTDMTAAAAAGARDRESIV

Target ORF information:

RefSeq Version	XM_002135565.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA28220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002135565.2

ORF Insert Sequence:

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCAGTGCG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC AGTTGGCTAT CTGACGGCTC TCACTGGCGA GCTCAAGACG 
CGTCAGGGCT TGGCCATATC CGGTGCGCTG ACGATGGCCC TGGATGAGGT CAACAAGGAT 
CCGAATCTGC TGCCAAATGT GACGCTCGAC CTGCGCTGGA ATGACACCAA GGGTGATACG 
GTATTGGCCA CCAAAGCCAT TACCGAAATG ATATGCGATG GCATTGCCAC CATATTCGGG 
CCGGAGGGTC CTTGTTATGT GGAGGCCATC GTGTCGCAGA GTCGCAACAT TCCAATGATA 
TCCTATAAAT GCGCAGAGTA CCGTGCATCC GCCATACCGA CGTTCGCCCG CACGGAACCG 
CCAGACACGC AGGTCGTCAA ATCGCTGCTC GCCTTGTTGC GCTACTATGC CTGGAACAAG 
TTCTCCATCC TGTACGAGGA CGTGTGGGGC CCGGTGGCGG ACTTGCTCAA GGATCAGGCC 
ACCAAGCGGA ACATGACGAT CAACCACAAG CAGTCGTTCA TCGACAATCG GGCCAAATGC 
TGCGAACTGG GGCTCGATTG CTGTCGCTCC GGCTATTGGT ATCAGCTGGT CCAGAATACG 
ATGAACCGCA CGAGGATCTA CGTGTTTCTG GGTGCCGCCA ACAGCCTGGT GGACTTTATG 
AGCTCCATGG AGACGGCCGG ACTGTTTGCG CGCGGCGAAT ACATGGTGAT CTTTGTGGAC 
ATGATGGTGT ACTCGGAACG TGAGGCAGAG AAGTACTTGC GGAAAGTGGA TCAGATCGAT 
CTCATGTCCA ACTGCCACAG CACCGAGAAG TTCAACCAGC TGGCACGCAG TCTGCTCGTC 
GTGGCCTCCA CGCCGCCGAC CAAGGGCTAT GAGAAGTTCA CAGAACGCGT GCGGACCTAC 
AGCTCGATGC CGCCGTTCAA CTTTGAGATA CCCAAACTGT TTTCCCAAAG TAAATTTAGC 
AAGTTCATAT CGATCTATGC CGCCTATCTG TACGATTCGG TGAAACTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGCCA GGAGACGCGT GTGCTCACCG ACGAGGTGAT CTTCGATGTG 
GCCAGCAATG GGACGCGTGT CATCGATACC ATCATCAAGA ATCGTACTTA TATGAGCGTC 
ACTGGGTCCA CGATCAAGAT CGATCGTTAT GGCGACTCGG AGGGGAACTT CTCAGTGCTG 
GCCTACAAGC CCCACAAATG GAACAACTCG AACGGAGTGA TATGCCACTA CCACATGGTG 
CCCGTGGCGT ATTTCCATCA AGGCGAAGAA CTTCCAGAAT ACAAACTGAT CAACGGCTCT 
ATAGACTGGC CCTCGGGCGG CGAGAAGCCC ACGGATGAGC CCATGTGCGG GTTTGCCAAC 
GAACTGTGCA AAAAGGACGA CACACACTAT ACCTCCACAG TGGCTGCCGT GGTGCTGGGG 
GTGCTGCTCT TCTGCTCTGG CGTGATCACC ATGAGCATCT ATCGCAAGTG GAAGATTGAG 
CTGGAGATCG AGGGTTTGCT GTGGAAGATC GATCCAAACG AAATCAAGGG CTACTCCGGC 
AACGAGATTG TCTCCTCGCC CAGCAAGGTC AGTTTGATGA GCGCCCAGAG CTATGGCTCA 
CGCTGGACAA ATCAGTTTGT GACCTCCACG GGTCGCCTAC GAGGCGCAGT GGTGCGCATC 
AAAGAATTGA AGTTCCCGCG CAAGCGGGAC ATCTCCAGGG AGATCATGAA GGAGATGCGT 
TTGCTGCGCG AGCTGCGACA CGACAATATC AACAGCTTCA TTGGCGCCTC TGTGGAGCCG 
ACGCGCATCC TGCTAGTCAC CGATTACTGC GCCAAGGGCA GTCTGTACGA TATCATCGAG 
AACGAAGACA TCAAATTGGA CGATCTGTTC ATTGCCTCAC TCATCCATGA CCTCATCAAG 
GGCATGATCT ACATCCACAA TTCCCAATTG ATCTATCATG GCAATCTCAA ATCCTCGAAT 
TGTGTGGTCA CCTCGCGCTG GATGTTGCAA GTCACCGACT TTGGGCTGCA CGAGCTGCGC 
CAGTGCGCCG AGAACGAGTC GATTGGGGAG CATCAGCATT ACCGAAATCA ACTGTGGCGC 
GCACCGGAAC TGCTGCGGAA TCATATCCAT GGCAGCCAGA AGGGCGATGT CTATGCGTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAC CATTTTAGAA 
CCAAAGGATA TTGTGGACTT TGTTAAGAAG CTGCCGCTCA ATGGCCAGGA GCCGTTTCGG 
CCGGAGGTCG AGTCCATAAT AGAGGCCGAA TCCTGTCCGG ATTATGTGCT GGCCTGCATC 
AAGGACTGCT GGGCCGAGGA GCCCGAAGAG CGGCCCGAAT TCAGTGCCAT ACGAAATCGC 
TTGAAGAAGA TGCGCGGCGG TAAAACCAAA AACATCATGG ACCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACCT GGAGGACATT GTCACCGAGC GCACACGTCT GCTGTGCGAG 
GAGAAGCTCA AGACAGAGGA TCTGCTGCAT CGCATGTTGC CCCAGTCGGT GGCCGAGAAG 
CTGACCATGG GTCAGGGCGT TGAGCCGGTG TCCTATGATT TGGTAACGAT CTATTTCAGC 
GACATTGTCG GCTTCACGGC CATGTCAGCG GAGAGCACAC CGCTGCAGGT GGTTAACTTT 
CTGAATGATC TCTACACGGT GTTCGATCGC ATCATTCGCG GCTATGATGT GTACAAGGTG 
GAGACCATAG GCGATGCCTA CATGGTGGTC TCTGGTCTCC CCATTAAGAA TGGGGATCGT 
CATGCGGGCG AGATAGCTTC GATGGCTCTG GAGCTGCTGC AGGCGGTGAA ACAGCATCGC 
ATCGCCCATC GTCCGAACGA GACGCTGAAG CTGAGAATCG GAATGCATAC GGGCCCCGTG 
GTGGCCGGCG TCGTGGGCCT CACGATGCCG AGGTACTGCC TCTTTGGCGA CACCGTGAAC 
ACGGCCTCAC GAATGGAGAG CAATGGCGAG GCGCTGAAGA TACACATTTC CAACAAGTGT 
AAGCTGGCGC TGGACAAACT CGGAGGCGGC TACATCACCG AGAAGCGTGG CCTGGTCAAT 
ATGAAGGGAA AGGGCGATGT GATCACCTGG TGGCTGACGG GGGCCAACGA TATTGCGATA 
CAGAAGAAGC CTGTGGACAT GATGGACATG CCGCCCCTGT TCTCCCGTCC GAGGAAGAGT 
CCGAAGCTGA ACTCCGACTC CAGGCAGCCA AGTATTCAGG CCATGCACTT CTGCGGGACA 
GGATCGAGGC GTCAGAGCAG TGTGCCAAGG CCGGCGGATG TCGAGTCCAC GTACAGCCTG 
CAGGGGTCCG TGAGGGAGTC GCCGCGAGTC AGCAAGAGGG ACAGGGACAG GGAGCGACCC 
GTCAATGGAC TGGGTGGAGC ACACTTTGTG GGCGGAGCAC TCCTGGAGTC GGCTCTGGCC 
TCGCTCAGCA CGCTGAATCA TTCCGAAACA AATGAAACGA ACTGTGATAT GGATGGAGGA 
GTAGGAGGAG CGAGTCTAGT ACGCCAGCCA AACGCTCTGC ACAAGCCCCT GGCCATGGTG 
CGGCCCCACC GGATCATCAG TGCCGCCCAG CTGCCCCTCG GGGAGGACGA CGACGCCGAC 
GGCACCACCG TCTCCCAGAT GGACGTGGAG ATGCTCCTGA GGGAATCTCG CTCCCTGGAT 
CCCATGCCGT GGCAGCATCT GCGCAAGCGG CACGAGAGGG TCAAGCTGCC ACCCTCCAAG 
CTGTCCAAAA ACAATTCGCG ATCCCTGGAC ACGGGAGTGT CGCTGATCAG CGGCAACCAG 
AACGGCGATG CCGAGGGGAG CCAACTGGAG ATCGACGACG ACATGTCCGC CAACCCAGTG 
GATGCCAACG ATAGCTATGA CGATGAGCTG GGCCTGCTCA TGCGACACGA CAACGGCCAG 
CTGCCCACGC TCCGCTACTC CGGCAGCTTC CCCAATGCCA ATGCCCACGC CCATGGCCAG 
ATCAGCATCG TTCCCACCGG CGGCAGCTCC AGCCGACGGC GCACCAGCAA TGTCTCCAGC 
GGCAGCTGTG CCAAGCATCT GAACAACAAC TGCAACGGCG GCATGAATAT CGAGGACGAT 
CTGGAGTCTC CCCTGCTGCA GCGCCAGGCC TCGCTCTCTG TGCCCCCCGA GGAAATGTTG 
GCGCACAACA AGCGCTGGCA CTCGCTAGAG CACATGGAGC CGGGCGGTGC CGGGCACGGG 
AACAGTGTCA GCTATGCGGC GGACATCGAT GGCCCTGTCA GTGGCTTGGA CATCTTGGGC 
GGCTCCTCCT CCAATCAGCG TCCCAGAGGT GTCGGTGGCA CAGGCGGCGG AGCCACCAAG 
CTGGGCAACT GGATGGCCAA TTTCTTCAAG GGCAATGGAG TGCGCAGCGG GGAGGCTAGG 
CGAGTGGGAA TCCTGCCCAC CGGTGTGCAA GGAGTAAGAG CTGGATTCAC GGATATGACG 
GCAGCGGCAG CAGCCGGAGC CCGAGATCGT GAGAGTATAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA28220 ( XM_015188565.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015044051.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA28220), mRNA.

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CloneID	ODe12365
Clone ID Related Accession (Same CDS sequence)	XM_002135565.2 , XM_015188565.1
Accession Version	XM_015188565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589954). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCAGTGCG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC AGTTGGCTAT CTGACGGCTC TCACTGGCGA GCTCAAGACG 
CGTCAGGGCT TGGCCATATC CGGTGCGCTG ACGATGGCCC TGGATGAGGT CAACAAGGAT 
CCGAATCTGC TGCCAAATGT GACGCTCGAC CTGCGCTGGA ATGACACCAA GGGTGATACG 
GTATTGGCCA CCAAAGCCAT TACCGAAATG ATATGCGATG GCATTGCCAC CATATTCGGG 
CCGGAGGGTC CTTGTTATGT GGAGGCCATC GTGTCGCAGA GTCGCAACAT TCCAATGATA 
TCCTATAAAT GCGCAGAGTA CCGTGCATCC GCCATACCGA CGTTCGCCCG CACGGAACCG 
CCAGACACGC AGGTCGTCAA ATCGCTGCTC GCCTTGTTGC GCTACTATGC CTGGAACAAG 
TTCTCCATCC TGTACGAGGA CGTGTGGGGC CCGGTGGCGG ACTTGCTCAA GGATCAGGCC 
ACCAAGCGGA ACATGACGAT CAACCACAAG CAGTCGTTCA TCGACAATCG GGCCAAATGC 
TGCGAACTGG GGCTCGATTG CTGTCGCTCC GGCTATTGGT ATCAGCTGGT CCAGAATACG 
ATGAACCGCA CGAGGATCTA CGTGTTTCTG GGTGCCGCCA ACAGCCTGGT GGACTTTATG 
AGCTCCATGG AGACGGCCGG ACTGTTTGCG CGCGGCGAAT ACATGGTGAT CTTTGTGGAC 
ATGATGGTGT ACTCGGAACG TGAGGCAGAG AAGTACTTGC GGAAAGTGGA TCAGATCGAT 
CTCATGTCCA ACTGCCACAG CACCGAGAAG TTCAACCAGC TGGCACGCAG TCTGCTCGTC 
GTGGCCTCCA CGCCGCCGAC CAAGGGCTAT GAGAAGTTCA CAGAACGCGT GCGGACCTAC 
AGCTCGATGC CGCCGTTCAA CTTTGAGATA CCCAAACTGT TTTCCCAAAG TAAATTTAGC 
AAGTTCATAT CGATCTATGC CGCCTATCTG TACGATTCGG TGAAACTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGCCA GGAGACGCGT GTGCTCACCG ACGAGGTGAT CTTCGATGTG 
GCCAGCAATG GGACGCGTGT CATCGATACC ATCATCAAGA ATCGTACTTA TATGAGCGTC 
ACTGGGTCCA CGATCAAGAT CGATCGTTAT GGCGACTCGG AGGGGAACTT CTCAGTGCTG 
GCCTACAAGC CCCACAAATG GAACAACTCG AACGGAGTGA TATGCCACTA CCACATGGTG 
CCCGTGGCGT ATTTCCATCA AGGCGAAGAA CTTCCAGAAT ACAAACTGAT CAACGGCTCT 
ATAGACTGGC CCTCGGGCGG CGAGAAGCCC ACGGATGAGC CCATGTGCGG GTTTGCCAAC 
GAACTGTGCA AAAAGGACGA CACACACTAT ACCTCCACAG TGGCTGCCGT GGTGCTGGGG 
GTGCTGCTCT TCTGCTCTGG CGTGATCACC ATGAGCATCT ATCGCAAGTG GAAGATTGAG 
CTGGAGATCG AGGGTTTGCT GTGGAAGATC GATCCAAACG AAATCAAGGG CTACTCCGGC 
AACGAGATTG TCTCCTCGCC CAGCAAGGTC AGTTTGATGA GCGCCCAGAG CTATGGCTCA 
CGCTGGACAA ATCAGTTTGT GACCTCCACG GGTCGCCTAC GAGGCGCAGT GGTGCGCATC 
AAAGAATTGA AGTTCCCGCG CAAGCGGGAC ATCTCCAGGG AGATCATGAA GGAGATGCGT 
TTGCTGCGCG AGCTGCGACA CGACAATATC AACAGCTTCA TTGGCGCCTC TGTGGAGCCG 
ACGCGCATCC TGCTAGTCAC CGATTACTGC GCCAAGGGCA GTCTGTACGA TATCATCGAG 
AACGAAGACA TCAAATTGGA CGATCTGTTC ATTGCCTCAC TCATCCATGA CCTCATCAAG 
GGCATGATCT ACATCCACAA TTCCCAATTG ATCTATCATG GCAATCTCAA ATCCTCGAAT 
TGTGTGGTCA CCTCGCGCTG GATGTTGCAA GTCACCGACT TTGGGCTGCA CGAGCTGCGC 
CAGTGCGCCG AGAACGAGTC GATTGGGGAG CATCAGCATT ACCGAAATCA ACTGTGGCGC 
GCACCGGAAC TGCTGCGGAA TCATATCCAT GGCAGCCAGA AGGGCGATGT CTATGCGTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAC CATTTTAGAA 
CCAAAGGATA TTGTGGACTT TGTTAAGAAG CTGCCGCTCA ATGGCCAGGA GCCGTTTCGG 
CCGGAGGTCG AGTCCATAAT AGAGGCCGAA TCCTGTCCGG ATTATGTGCT GGCCTGCATC 
AAGGACTGCT GGGCCGAGGA GCCCGAAGAG CGGCCCGAAT TCAGTGCCAT ACGAAATCGC 
TTGAAGAAGA TGCGCGGCGG TAAAACCAAA AACATCATGG ACCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACCT GGAGGACATT GTCACCGAGC GCACACGTCT GCTGTGCGAG 
GAGAAGCTCA AGACAGAGGA TCTGCTGCAT CGCATGTTGC CCCAGTCGGT GGCCGAGAAG 
CTGACCATGG GTCAGGGCGT TGAGCCGGTG TCCTATGATT TGGTAACGAT CTATTTCAGC 
GACATTGTCG GCTTCACGGC CATGTCAGCG GAGAGCACAC CGCTGCAGGT GGTTAACTTT 
CTGAATGATC TCTACACGGT GTTCGATCGC ATCATTCGCG GCTATGATGT GTACAAGGTG 
GAGACCATAG GCGATGCCTA CATGGTGGTC TCTGGTCTCC CCATTAAGAA TGGGGATCGT 
CATGCGGGCG AGATAGCTTC GATGGCTCTG GAGCTGCTGC AGGCGGTGAA ACAGCATCGC 
ATCGCCCATC GTCCGAACGA GACGCTGAAG CTGAGAATCG GAATGCATAC GGGCCCCGTG 
GTGGCCGGCG TCGTGGGCCT CACGATGCCG AGGTACTGCC TCTTTGGCGA CACCGTGAAC 
ACGGCCTCAC GAATGGAGAG CAATGGCGAG GCGCTGAAGA TACACATTTC CAACAAGTGT 
AAGCTGGCGC TGGACAAACT CGGAGGCGGC TACATCACCG AGAAGCGTGG CCTGGTCAAT 
ATGAAGGGAA AGGGCGATGT GATCACCTGG TGGCTGACGG GGGCCAACGA TATTGCGATA 
CAGAAGAAGC CTGTGGACAT GATGGACATG CCGCCCCTGT TCTCCCGTCC GAGGAAGAGT 
CCGAAGCTGA ACTCCGACTC CAGGCAGCCA AGTATTCAGG CCATGCACTT CTGCGGGACA 
GGATCGAGGC GTCAGAGCAG TGTGCCAAGG CCGGCGGATG TCGAGTCCAC GTACAGCCTG 
CAGGGGTCCG TGAGGGAGTC GCCGCGAGTC AGCAAGAGGG ACAGGGACAG GGAGCGACCC 
GTCAATGGAC TGGGTGGAGC ACACTTTGTG GGCGGAGCAC TCCTGGAGTC GGCTCTGGCC 
TCGCTCAGCA CGCTGAATCA TTCCGAAACA AATGAAACGA ACTGTGATAT GGATGGAGGA 
GTAGGAGGAG CGAGTCTAGT ACGCCAGCCA AACGCTCTGC ACAAGCCCCT GGCCATGGTG 
CGGCCCCACC GGATCATCAG TGCCGCCCAG CTGCCCCTCG GGGAGGACGA CGACGCCGAC 
GGCACCACCG TCTCCCAGAT GGACGTGGAG ATGCTCCTGA GGGAATCTCG CTCCCTGGAT 
CCCATGCCGT GGCAGCATCT GCGCAAGCGG CACGAGAGGG TCAAGCTGCC ACCCTCCAAG 
CTGTCCAAAA ACAATTCGCG ATCCCTGGAC ACGGGAGTGT CGCTGATCAG CGGCAACCAG 
AACGGCGATG CCGAGGGGAG CCAACTGGAG ATCGACGACG ACATGTCCGC CAACCCAGTG 
GATGCCAACG ATAGCTATGA CGATGAGCTG GGCCTGCTCA TGCGACACGA CAACGGCCAG 
CTGCCCACGC TCCGCTACTC CGGCAGCTTC CCCAATGCCA ATGCCCACGC CCATGGCCAG 
ATCAGCATCG TTCCCACCGG CGGCAGCTCC AGCCGACGGC GCACCAGCAA TGTCTCCAGC 
GGCAGCTGTG CCAAGCATCT GAACAACAAC TGCAACGGCG GCATGAATAT CGAGGACGAT 
CTGGAGTCTC CCCTGCTGCA GCGCCAGGCC TCGCTCTCTG TGCCCCCCGA GGAAATGTTG 
GCGCACAACA AGCGCTGGCA CTCGCTAGAG CACATGGAGC CGGGCGGTGC CGGGCACGGG 
AACAGTGTCA GCTATGCGGC GGACATCGAT GGCCCTGTCA GTGGCTTGGA CATCTTGGGC 
GGCTCCTCCT CCAATCAGCG TCCCAGAGGT GTCGGTGGCA CAGGCGGCGG AGCCACCAAG 
CTGGGCAACT GGATGGCCAA TTTCTTCAAG GGCAATGGAG TGCGCAGCGG GGAGGCTAGG 
CGAGTGGGAA TCCTGCCCAC CGGTGTGCAA GGAGTAAGAG CTGGATTCAC GGATATGACG 
GCAGCGGCAG CAGCCGGAGC CCGAGATCGT GAGAGTATAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015044051.1
CDS	444..5048
Translation	MTRWPFNLLLLLSVAVRDCSNHRTVLTVGYLTALTGELKTRQGLAISGALTMALDEVNKDPNLLPNVTLDLRWNDTKGDTVLATKAITEMICDGIATIFGPEGPCYVEAIVSQSRNIPMISYKCAEYRASAIPTFARTEPPDTQVVKSLLALLRYYAWNKFSILYEDVWGPVADLLKDQATKRNMTINHKQSFIDNRAKCCELGLDCCRSGYWYQLVQNTMNRTRIYVFLGAANSLVDFMSSMETAGLFARGEYMVIFVDMMVYSEREAEKYLRKVDQIDLMSNCHSTEKFNQLARSLLVVASTPPTKGYEKFTERVRTYSSMPPFNFEIPKLFSQSKFSKFISIYAAYLYDSVKLYAWAVDKMLRQETRVLTDEVIFDVASNGTRVIDTIIKNRTYMSVTGSTIKIDRYGDSEGNFSVLAYKPHKWNNSNGVICHYHMVPVAYFHQGEELPEYKLINGSIDWPSGGEKPTDEPMCGFANELCKKDDTHYTSTVAAVVLGVLLFCSGVITMSIYRKWKIELEIEGLLWKIDPNEIKGYSGNEIVSSPSKVSLMSAQSYGSRWTNQFVTSTGRLRGAVVRIKELKFPRKRDISREIMKEMRLLRELRHDNINSFIGASVEPTRILLVTDYCAKGSLYDIIENEDIKLDDLFIASLIHDLIKGMIYIHNSQLIYHGNLKSSNCVVTSRWMLQVTDFGLHELRQCAENESIGEHQHYRNQLWRAPELLRNHIHGSQKGDVYAFAIIMYEIFSRKGPFGQTILEPKDIVDFVKKLPLNGQEPFRPEVESIIEAESCPDYVLACIKDCWAEEPEERPEFSAIRNRLKKMRGGKTKNIMDQMMEMMEKYANNLEDIVTERTRLLCEEKLKTEDLLHRMLPQSVAEKLTMGQGVEPVSYDLVTIYFSDIVGFTAMSAESTPLQVVNFLNDLYTVFDRIIRGYDVYKVETIGDAYMVVSGLPIKNGDRHAGEIASMALELLQAVKQHRIAHRPNETLKLRIGMHTGPVVAGVVGLTMPRYCLFGDTVNTASRMESNGEALKIHISNKCKLALDKLGGGYITEKRGLVNMKGKGDVITWWLTGANDIAIQKKPVDMMDMPPLFSRPRKSPKLNSDSRQPSIQAMHFCGTGSRRQSSVPRPADVESTYSLQGSVRESPRVSKRDRDRERPVNGLGGAHFVGGALLESALASLSTLNHSETNETNCDMDGGVGGASLVRQPNALHKPLAMVRPHRIISAAQLPLGEDDDADGTTVSQMDVEMLLRESRSLDPMPWQHLRKRHERVKLPPSKLSKNNSRSLDTGVSLISGNQNGDAEGSQLEIDDDMSANPVDANDSYDDELGLLMRHDNGQLPTLRYSGSFPNANAHAHGQISIVPTGGSSSRRRTSNVSSGSCAKHLNNNCNGGMNIEDDLESPLLQRQASLSVPPEEMLAHNKRWHSLEHMEPGGAGHGNSVSYAADIDGPVSGLDILGGSSSNQRPRGVGGTGGGATKLGNWMANFFKGNGVRSGEARRVGILPTGVQGVRAGFTDMTAAAAAGARDRESIV

Target ORF information:

RefSeq Version	XM_015188565.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA28220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015188565.1

ORF Insert Sequence:

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCAGTGCG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC AGTTGGCTAT CTGACGGCTC TCACTGGCGA GCTCAAGACG 
CGTCAGGGCT TGGCCATATC CGGTGCGCTG ACGATGGCCC TGGATGAGGT CAACAAGGAT 
CCGAATCTGC TGCCAAATGT GACGCTCGAC CTGCGCTGGA ATGACACCAA GGGTGATACG 
GTATTGGCCA CCAAAGCCAT TACCGAAATG ATATGCGATG GCATTGCCAC CATATTCGGG 
CCGGAGGGTC CTTGTTATGT GGAGGCCATC GTGTCGCAGA GTCGCAACAT TCCAATGATA 
TCCTATAAAT GCGCAGAGTA CCGTGCATCC GCCATACCGA CGTTCGCCCG CACGGAACCG 
CCAGACACGC AGGTCGTCAA ATCGCTGCTC GCCTTGTTGC GCTACTATGC CTGGAACAAG 
TTCTCCATCC TGTACGAGGA CGTGTGGGGC CCGGTGGCGG ACTTGCTCAA GGATCAGGCC 
ACCAAGCGGA ACATGACGAT CAACCACAAG CAGTCGTTCA TCGACAATCG GGCCAAATGC 
TGCGAACTGG GGCTCGATTG CTGTCGCTCC GGCTATTGGT ATCAGCTGGT CCAGAATACG 
ATGAACCGCA CGAGGATCTA CGTGTTTCTG GGTGCCGCCA ACAGCCTGGT GGACTTTATG 
AGCTCCATGG AGACGGCCGG ACTGTTTGCG CGCGGCGAAT ACATGGTGAT CTTTGTGGAC 
ATGATGGTGT ACTCGGAACG TGAGGCAGAG AAGTACTTGC GGAAAGTGGA TCAGATCGAT 
CTCATGTCCA ACTGCCACAG CACCGAGAAG TTCAACCAGC TGGCACGCAG TCTGCTCGTC 
GTGGCCTCCA CGCCGCCGAC CAAGGGCTAT GAGAAGTTCA CAGAACGCGT GCGGACCTAC 
AGCTCGATGC CGCCGTTCAA CTTTGAGATA CCCAAACTGT TTTCCCAAAG TAAATTTAGC 
AAGTTCATAT CGATCTATGC CGCCTATCTG TACGATTCGG TGAAACTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGCCA GGAGACGCGT GTGCTCACCG ACGAGGTGAT CTTCGATGTG 
GCCAGCAATG GGACGCGTGT CATCGATACC ATCATCAAGA ATCGTACTTA TATGAGCGTC 
ACTGGGTCCA CGATCAAGAT CGATCGTTAT GGCGACTCGG AGGGGAACTT CTCAGTGCTG 
GCCTACAAGC CCCACAAATG GAACAACTCG AACGGAGTGA TATGCCACTA CCACATGGTG 
CCCGTGGCGT ATTTCCATCA AGGCGAAGAA CTTCCAGAAT ACAAACTGAT CAACGGCTCT 
ATAGACTGGC CCTCGGGCGG CGAGAAGCCC ACGGATGAGC CCATGTGCGG GTTTGCCAAC 
GAACTGTGCA AAAAGGACGA CACACACTAT ACCTCCACAG TGGCTGCCGT GGTGCTGGGG 
GTGCTGCTCT TCTGCTCTGG CGTGATCACC ATGAGCATCT ATCGCAAGTG GAAGATTGAG 
CTGGAGATCG AGGGTTTGCT GTGGAAGATC GATCCAAACG AAATCAAGGG CTACTCCGGC 
AACGAGATTG TCTCCTCGCC CAGCAAGGTC AGTTTGATGA GCGCCCAGAG CTATGGCTCA 
CGCTGGACAA ATCAGTTTGT GACCTCCACG GGTCGCCTAC GAGGCGCAGT GGTGCGCATC 
AAAGAATTGA AGTTCCCGCG CAAGCGGGAC ATCTCCAGGG AGATCATGAA GGAGATGCGT 
TTGCTGCGCG AGCTGCGACA CGACAATATC AACAGCTTCA TTGGCGCCTC TGTGGAGCCG 
ACGCGCATCC TGCTAGTCAC CGATTACTGC GCCAAGGGCA GTCTGTACGA TATCATCGAG 
AACGAAGACA TCAAATTGGA CGATCTGTTC ATTGCCTCAC TCATCCATGA CCTCATCAAG 
GGCATGATCT ACATCCACAA TTCCCAATTG ATCTATCATG GCAATCTCAA ATCCTCGAAT 
TGTGTGGTCA CCTCGCGCTG GATGTTGCAA GTCACCGACT TTGGGCTGCA CGAGCTGCGC 
CAGTGCGCCG AGAACGAGTC GATTGGGGAG CATCAGCATT ACCGAAATCA ACTGTGGCGC 
GCACCGGAAC TGCTGCGGAA TCATATCCAT GGCAGCCAGA AGGGCGATGT CTATGCGTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAC CATTTTAGAA 
CCAAAGGATA TTGTGGACTT TGTTAAGAAG CTGCCGCTCA ATGGCCAGGA GCCGTTTCGG 
CCGGAGGTCG AGTCCATAAT AGAGGCCGAA TCCTGTCCGG ATTATGTGCT GGCCTGCATC 
AAGGACTGCT GGGCCGAGGA GCCCGAAGAG CGGCCCGAAT TCAGTGCCAT ACGAAATCGC 
TTGAAGAAGA TGCGCGGCGG TAAAACCAAA AACATCATGG ACCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACCT GGAGGACATT GTCACCGAGC GCACACGTCT GCTGTGCGAG 
GAGAAGCTCA AGACAGAGGA TCTGCTGCAT CGCATGTTGC CCCAGTCGGT GGCCGAGAAG 
CTGACCATGG GTCAGGGCGT TGAGCCGGTG TCCTATGATT TGGTAACGAT CTATTTCAGC 
GACATTGTCG GCTTCACGGC CATGTCAGCG GAGAGCACAC CGCTGCAGGT GGTTAACTTT 
CTGAATGATC TCTACACGGT GTTCGATCGC ATCATTCGCG GCTATGATGT GTACAAGGTG 
GAGACCATAG GCGATGCCTA CATGGTGGTC TCTGGTCTCC CCATTAAGAA TGGGGATCGT 
CATGCGGGCG AGATAGCTTC GATGGCTCTG GAGCTGCTGC AGGCGGTGAA ACAGCATCGC 
ATCGCCCATC GTCCGAACGA GACGCTGAAG CTGAGAATCG GAATGCATAC GGGCCCCGTG 
GTGGCCGGCG TCGTGGGCCT CACGATGCCG AGGTACTGCC TCTTTGGCGA CACCGTGAAC 
ACGGCCTCAC GAATGGAGAG CAATGGCGAG GCGCTGAAGA TACACATTTC CAACAAGTGT 
AAGCTGGCGC TGGACAAACT CGGAGGCGGC TACATCACCG AGAAGCGTGG CCTGGTCAAT 
ATGAAGGGAA AGGGCGATGT GATCACCTGG TGGCTGACGG GGGCCAACGA TATTGCGATA 
CAGAAGAAGC CTGTGGACAT GATGGACATG CCGCCCCTGT TCTCCCGTCC GAGGAAGAGT 
CCGAAGCTGA ACTCCGACTC CAGGCAGCCA AGTATTCAGG CCATGCACTT CTGCGGGACA 
GGATCGAGGC GTCAGAGCAG TGTGCCAAGG CCGGCGGATG TCGAGTCCAC GTACAGCCTG 
CAGGGGTCCG TGAGGGAGTC GCCGCGAGTC AGCAAGAGGG ACAGGGACAG GGAGCGACCC 
GTCAATGGAC TGGGTGGAGC ACACTTTGTG GGCGGAGCAC TCCTGGAGTC GGCTCTGGCC 
TCGCTCAGCA CGCTGAATCA TTCCGAAACA AATGAAACGA ACTGTGATAT GGATGGAGGA 
GTAGGAGGAG CGAGTCTAGT ACGCCAGCCA AACGCTCTGC ACAAGCCCCT GGCCATGGTG 
CGGCCCCACC GGATCATCAG TGCCGCCCAG CTGCCCCTCG GGGAGGACGA CGACGCCGAC 
GGCACCACCG TCTCCCAGAT GGACGTGGAG ATGCTCCTGA GGGAATCTCG CTCCCTGGAT 
CCCATGCCGT GGCAGCATCT GCGCAAGCGG CACGAGAGGG TCAAGCTGCC ACCCTCCAAG 
CTGTCCAAAA ACAATTCGCG ATCCCTGGAC ACGGGAGTGT CGCTGATCAG CGGCAACCAG 
AACGGCGATG CCGAGGGGAG CCAACTGGAG ATCGACGACG ACATGTCCGC CAACCCAGTG 
GATGCCAACG ATAGCTATGA CGATGAGCTG GGCCTGCTCA TGCGACACGA CAACGGCCAG 
CTGCCCACGC TCCGCTACTC CGGCAGCTTC CCCAATGCCA ATGCCCACGC CCATGGCCAG 
ATCAGCATCG TTCCCACCGG CGGCAGCTCC AGCCGACGGC GCACCAGCAA TGTCTCCAGC 
GGCAGCTGTG CCAAGCATCT GAACAACAAC TGCAACGGCG GCATGAATAT CGAGGACGAT 
CTGGAGTCTC CCCTGCTGCA GCGCCAGGCC TCGCTCTCTG TGCCCCCCGA GGAAATGTTG 
GCGCACAACA AGCGCTGGCA CTCGCTAGAG CACATGGAGC CGGGCGGTGC CGGGCACGGG 
AACAGTGTCA GCTATGCGGC GGACATCGAT GGCCCTGTCA GTGGCTTGGA CATCTTGGGC 
GGCTCCTCCT CCAATCAGCG TCCCAGAGGT GTCGGTGGCA CAGGCGGCGG AGCCACCAAG 
CTGGGCAACT GGATGGCCAA TTTCTTCAAG GGCAATGGAG TGCGCAGCGG GGAGGCTAGG 
CGAGTGGGAA TCCTGCCCAC CGGTGTGCAA GGAGTAAGAG CTGGATTCAC GGATATGACG 
GCAGCGGCAG CAGCCGGAGC CCGAGATCGT GAGAGTATAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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Dpse\GA28220 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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