Dpse\GA16429 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA16429
Entrez Gene ID	4816480
Full Name	GA16429 gene product from transcript GA16429-RA
Synonyms	GA16429,dpse_GLEANR_8917
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001356212.2	XP_001356248.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA16429 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA16429 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe10706	XM_001356212.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA16429), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA16429 ( XM_001356212.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001356248.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA16429), mRNA.

Price & Availability↑

CloneID	ODe10706
Clone ID Related Accession (Same CDS sequence)	XM_001356212.2
Accession Version	XM_001356212.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589963). On Dec 22, 2015 this sequence version replaced XM_001356212.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTGCA GGGGCAGCCC ATTGTTCTTT GTGTTTCTCC GTAAAAACTT TCTATATTTG 
CGATTCAATT TGGTGCCTTT ACTGTGTATC TTTATTGGGT CTGCGGCTGT GCTATGTGGA 
TATTTGCACT GGTCGGTGAG ACCTGTGAAG CACAACTTGG AGGAGCTCGA ACCAAAAGAC 
TACGATGTAC TGAAACAACT TTACTTCCCT GCAAACGACC TGGACTATAT ATATTTTGCA 
CCCAGCAGTA ACAATGTCGA TGATATTATG GACATGGTGC GACAGGAGCT GGAGATCCCT 
GCAGAGCGTT TCCGTATCTA CAACATTAGC TCCGATATGC AGCTGGACAT GGAACAGAAC 
TGCCTGGGCA ACTGTTTCGC AATCAACTTT AATCAAGTGC CCAACCGGGG CTCTGTTGGA 
CGGCTCGTGT ATAGTCTGAG CTCAAATCAA ATGCGAATTT CGCCCAAGAA ACGATTCGTG 
AATGATGAAG AGATAAATCA TCAGAAGGAT GACGACGACT ACATACGGGT GGGTTTTCTG 
ACGCTGCAGC ACATATTGGA CCGCCAGTAT ATGAAGTATC AGGAACTAAA CCCCAACTAT 
GAGCTCCTGC TTAGTTCCAT GCCCAACCTG GAGGTTAGCA GCATGGACAG CCATCGTCTG 
ACCTACTTTG GCACACTATG CAGCATCCTC TTCAATGTCG TCCTGCTGAG CACTTTTGTT 
GTCCCTTTTG TGGAGGAAAA ACAGAACGGA CTCAAGGAGT TTCTAAATCT AGTCACTCCA 
ATGAGTTTCC TGAATGGCCT CACCTTCTTT TTCATTCGCT TTATCTGCTA TGCAGCGCTC 
CTTCTATTTG TCCTTATCGC CGCCTACTTT TACGAGGCTC TGGGCCTGAT TGGCTTCGCT 
TACATCGCTG TTTTGTATCT GCTTTACATA TTGGCCACTA TGTCGTACGC GTACTTAATC 
TCCGTGTGCT TTCACTCTGT TTTCTACGCG AAAATTGGCG GCCTGGTGAT GCTGATCATT 
CCCTACGTGT TCAGTTTTGT CCGCAGCTGG GTCACCTCTG TGGCCTTTTT CCTGTTTAGC 
ACCAATGCCT TCCTTGAGGG CTTGGATGTG TTCCAGACCT TCTCCAACAA ACATCGCCAC 
TTTGGGGCAG CGGATCTTTT CCGTGAGATC AAAGATGACT CCACCCGCAT GTTCTGGATC 
TACTGTTCAC TCATTGCTCA ATCCATCCTC TACGCTCTGC TTTACAACTA TATGACCTGT 
GTTTTCCCCG GTCCTGGGGG ACTCAAGCGT CCTTTTCTGT TCTTCCTGGA TCCCAATACG 
TACAAGAAGC GACAACGCAA TGAGTACACA ACTGCACCGC GTGGAACCCA TGCCATCATC 
ATATCGGAAC TGTGCAAGAA GTTCCAGACA AGTAAACGCG AAACGCTTAT TTCGGACAAT 
CTAAATATGA CGATTAACAA CAAGGAGATC ACTGTTCTCT TGGGCCACAA CGGAGCCGGA 
AAGACAACGA TGATGAACAT GATTATGGGT CTGGTCCCGA AGGATGCGGG CAAGATTGTT 
GTCTGTAGCG AGCGCGATGT GGCCTCGTAT CGCCACCTGA TTGGGTTCTG TCCCCAGCAC 
AGTGTTTTCA TGAGCTACAT GACGTGTCAT CAGCATTTGG AGTTCTTTGC CCAGCTGCGC 
GGGTCACGCC GCTCGGATGC GTGTCTTTGG GCTGATACCC AGCTCAAAAA ACTGGGCCTC 
AAAGAGAAAC GCGACGAGTA TGGGCGCAAT TTGTCAGGGG GAATGAAGAG GCGCCTGTCG 
CTGGGCATTG CCATTGCAGG AAACACAAAA ATTGTGATTC TGGACGAGCC CTCGTCTGGA 
CTGGACATCG AGTCCCGACG CCAGTTGTGG GACATCCTGT TGAATCTGCG CAAGGAGAAG 
GCCATTCTGG TCACCACCCA CTACATGGAG GAGGCCGAAG TGCTGGGTGA CACCATCTGC 
ATCTTGGCCA ACGGAAAGCT GCAGAGCACT GGCTCCCCTC TGGAACTGAA GCGTAGATTG 
GGCATCGGGT ACCGCCTAAA GCTGGAAGTC ACCGATTTCA CATTCCGCGA ACGTGAAATA 
ATGAAAATTA TACTCGATTA CGTTCCGACT GCCCGCGTGC TGAATGTGGT GAAGCCGACT 
GTCTACATTT GCCTGCCGTA TGCGTACAAG ATGTGTTTCT CGGAAATGCT GCTCAAACTG 
GAGTCGAGGA CAAGTGAGCT GGAAATAAGT ACCATTTCTA TGACGGATAC TTCACTAGAA 
GATGTTTTTC TAAGATGTGC GGGAGAGCAG GATCAGGTGG ACACTCCAGA CGGCTCTCGA 
AACGATGCTC CCCTGCTGAC CGCGCTCTAC AAGCGACTAC CCAGCCAGCA CACTCCGCCC 
AGCACGTGGC AGCTGTGGCT GGCGGTGTTC TACAAGAAGT GGACGTTCCT TGCCAACGAG 
TGGTTATATG CTGTCATAAT GCTGTCCCTT CCCGTTGTGT GCGTCTCTGG AGCCATTCTC 
GCTATGCACG CCATGTCTGT TGTGAAGAAT GAGGCGGCCC TACCGCTCAA GCTCAGTCAG 
ATGGGCTCTG GAGTAGTTTA TATATACAAT CCGACGGGCC ATCATGCTCA GGTTGAGCAA 
CAGCTGCGGC AGCAGATCGA ACTTAGTGGA CTCACGGCCA AAACGTTGCA GCTACGCGGA 
AACAGTGTGA AAAATGAGCT CCTCGAATTG CAACGCGACA ACTTGGCGGA TTTCCTGGAA 
CAGGTGGTGG GCGCTATCGA CATGTACGGC GGGGGCCGGG GCACTGGAGG AACTCCGCAG 
ATAGAAATCT TTTACGCGGG CAATCGCTAC CACAGCTCAG TGGAGCTGAT CAACTTTCTG 
GACTCCAGCA TGCTGAAGAT GGTCAGCCCA GAGTCGGACA TTGAAACCAC GTATGTGCCG 
ATTCGACGCT TCATCAGCGA CATCAGTCCC ACGCGATTGG AGTACTATGT GGTGATCGTG 
TCCGTGGGAA TGTTCTTCTG CATGTTCTAC TTCATTTCGC TACCCTTCCG AGAGCACGAC 
AACGGCTTCC GCCAGCTGCA GACCATGTCA CGCTACACCT TCTGGCTGTC CACCTTTGTG 
TTCGACGTCC TGCTGCTGGT CCTCCTCTGT GTGGCGCTTT TTGTGCTTCA GAACTGGCTA 
ATGCCTTCGG AGCTCTACTC CACCTCAGAA CTGAGAATCA TTGTTATGAG CATATTCTTC 
TACGGCTGCA GCTACCTGCC CATTCTGTAC TCGCTGGCCA ATAACTTCAA GTCCATATCG 
ACGATATCCA CCTACCTGCT GCTGATGCTG ATTGTGTCCG CAATTGCTCC TCTGATCACC 
TCCAACAATG CGCAGTCCAT AAAACTGCAC GAGACGAAGA TAGCCTTCCT CTGCTTCCTG 
CCCGACTTCA ATCTGAACCA CCAGCTGCGC ATCATCAATG AGAATTTCAT TGCTAACCGA 
CGCTCTGTAC CCACCGCCCA TTTGCTTTCG ACGGATACGT TCTTTGTCTA TGCCATGGCC 
GTCTGCGTGC TGGTCTTTTC ATTCTTCACC ATCGTTTTGG AGCACAAGTA CCTCCGCCAA 
CGGGTCCACG ATGCCATCTG GATGAACAGG TGCCTGGTCA CCTTGAAGAA GAACAGCTCG 
ACGGGCACCA GTTCTAGCAC CCAGCTCACG GTCGTTGACG ACTGTGCCAA CGAAGAGCGT 
CGAGTGGAGG CGCTCATGAT CGATCCGACG CAGGAGTGTC CCTTGATCGT CAGCAAAGTG 
CGCAAGCGGT ACCACAAGAA AGTGGTGGTG GATGGAATCA GTTTTGCCGC TTCTAGAGGC 
GAATGCTTTG GCCTCTTAGG CATCAATGGA GCCGGAAAGA CGAGCACATT CGAAATGATT 
GCCGCCAATC TGCCTCTGGA CGGAGGGAGC ATACGAATCG ATGGCATCGA TATTCGAAAG 
GACGAAGTAG GCTATCGCCA TCGTTTCGGC TACTGCCCCC AGTACGATGC CCTGAATAAG 
TTTATGACCG CCGAACAATG TCTCCACTAT ATGGCCCTCC TGCGGGGACT GAGCACCACC 
AGCGAAGGAC CGAGTAGCGT GACGGAAAAC GTGAAATACT GGCTCGAAAA AATGCACCTG 
ACCAAGTATC AACAGGTCCA GGTGCGACAT TATTCGGGGG GGACGAAGCG CAAACTGATG 
GCTGCCATGG CGATGATCGG GGCCCCGACA CTGGTTCTCC TAGACGAGCC CACGACTGGC 
GTGGATCCCA TTTCCAGACG CTTTCTGTGG GAGTGCATCA AGGACTTTCA GGGCCAGGAG 
CGCACTGTGG TGCTTACTTC ACACAGCATG GACGAGTGTG AGGAGCTGTG TAATCGCCTG 
GCCATCATGG CCAATGGGAA ATTCAAGTGC CTGAATACCA TCTGCGCCTT GAAGAGACTG 
AGCGGCTTCA CCATAAAACT TAAGATGAAA CTAGACACGG AAACGGAGCA GAACGTGCAC 
ACTATCACAA ATACCCTGAA GGCGCAGTTC GAGGGTCTGG AGTTGCGCGA GAATCATGCC 
GGAACCCTAA CCTACTTTGT TAGCACCCAG GAGAACTTGG TCTATTGGTC GAGGGTGTTT 
AGGATAACCG AGGACCATCT GACCGACAGA TTGGGCCACA TCGTGGCGGA CTACTCGCTG 
AATGAGTGCA CTTTGGAAGA TATTTTTCTC AAATTCGACA AAAAATCGAA GACCCCACCC 
TCATCGCAAA CATCGTCGCG CAACTCCCCA GTCCAACACA ACCCTGACGC GTCCGACTTT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001356248.1
CDS	170..4975
Translation	MACRGSPLFFVFLRKNFLYLRFNLVPLLCIFIGSAAVLCGYLHWSVRPVKHNLEELEPKDYDVLKQLYFPANDLDYIYFAPSSNNVDDIMDMVRQELEIPAERFRIYNISSDMQLDMEQNCLGNCFAINFNQVPNRGSVGRLVYSLSSNQMRISPKKRFVNDEEINHQKDDDDYIRVGFLTLQHILDRQYMKYQELNPNYELLLSSMPNLEVSSMDSHRLTYFGTLCSILFNVVLLSTFVVPFVEEKQNGLKEFLNLVTPMSFLNGLTFFFIRFICYAALLLFVLIAAYFYEALGLIGFAYIAVLYLLYILATMSYAYLISVCFHSVFYAKIGGLVMLIIPYVFSFVRSWVTSVAFFLFSTNAFLEGLDVFQTFSNKHRHFGAADLFREIKDDSTRMFWIYCSLIAQSILYALLYNYMTCVFPGPGGLKRPFLFFLDPNTYKKRQRNEYTTAPRGTHAIIISELCKKFQTSKRETLISDNLNMTINNKEITVLLGHNGAGKTTMMNMIMGLVPKDAGKIVVCSERDVASYRHLIGFCPQHSVFMSYMTCHQHLEFFAQLRGSRRSDACLWADTQLKKLGLKEKRDEYGRNLSGGMKRRLSLGIAIAGNTKIVILDEPSSGLDIESRRQLWDILLNLRKEKAILVTTHYMEEAEVLGDTICILANGKLQSTGSPLELKRRLGIGYRLKLEVTDFTFREREIMKIILDYVPTARVLNVVKPTVYICLPYAYKMCFSEMLLKLESRTSELEISTISMTDTSLEDVFLRCAGEQDQVDTPDGSRNDAPLLTALYKRLPSQHTPPSTWQLWLAVFYKKWTFLANEWLYAVIMLSLPVVCVSGAILAMHAMSVVKNEAALPLKLSQMGSGVVYIYNPTGHHAQVEQQLRQQIELSGLTAKTLQLRGNSVKNELLELQRDNLADFLEQVVGAIDMYGGGRGTGGTPQIEIFYAGNRYHSSVELINFLDSSMLKMVSPESDIETTYVPIRRFISDISPTRLEYYVVIVSVGMFFCMFYFISLPFREHDNGFRQLQTMSRYTFWLSTFVFDVLLLVLLCVALFVLQNWLMPSELYSTSELRIIVMSIFFYGCSYLPILYSLANNFKSISTISTYLLLMLIVSAIAPLITSNNAQSIKLHETKIAFLCFLPDFNLNHQLRIINENFIANRRSVPTAHLLSTDTFFVYAMAVCVLVFSFFTIVLEHKYLRQRVHDAIWMNRCLVTLKKNSSTGTSSSTQLTVVDDCANEERRVEALMIDPTQECPLIVSKVRKRYHKKVVVDGISFAASRGECFGLLGINGAGKTSTFEMIAANLPLDGGSIRIDGIDIRKDEVGYRHRFGYCPQYDALNKFMTAEQCLHYMALLRGLSTTSEGPSSVTENVKYWLEKMHLTKYQQVQVRHYSGGTKRKLMAAMAMIGAPTLVLLDEPTTGVDPISRRFLWECIKDFQGQERTVVLTSHSMDECEELCNRLAIMANGKFKCLNTICALKRLSGFTIKLKMKLDTETEQNVHTITNTLKAQFEGLELRENHAGTLTYFVSTQENLVYWSRVFRITEDHLTDRLGHIVADYSLNECTLEDIFLKFDKKSKTPPSSQTSSRNSPVQHNPDASDFV

Target ORF information:

RefSeq Version	XM_001356212.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA16429), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001356212.2

ORF Insert Sequence:

ATGGCTTGCA GGGGCAGCCC ATTGTTCTTT GTGTTTCTCC GTAAAAACTT TCTATATTTG 
CGATTCAATT TGGTGCCTTT ACTGTGTATC TTTATTGGGT CTGCGGCTGT GCTATGTGGA 
TATTTGCACT GGTCGGTGAG ACCTGTGAAG CACAACTTGG AGGAGCTCGA ACCAAAAGAC 
TACGATGTAC TGAAACAACT TTACTTCCCT GCAAACGACC TGGACTATAT ATATTTTGCA 
CCCAGCAGTA ACAATGTCGA TGATATTATG GACATGGTGC GACAGGAGCT GGAGATCCCT 
GCAGAGCGTT TCCGTATCTA CAACATTAGC TCCGATATGC AGCTGGACAT GGAACAGAAC 
TGCCTGGGCA ACTGTTTCGC AATCAACTTT AATCAAGTGC CCAACCGGGG CTCTGTTGGA 
CGGCTCGTGT ATAGTCTGAG CTCAAATCAA ATGCGAATTT CGCCCAAGAA ACGATTCGTG 
AATGATGAAG AGATAAATCA TCAGAAGGAT GACGACGACT ACATACGGGT GGGTTTTCTG 
ACGCTGCAGC ACATATTGGA CCGCCAGTAT ATGAAGTATC AGGAACTAAA CCCCAACTAT 
GAGCTCCTGC TTAGTTCCAT GCCCAACCTG GAGGTTAGCA GCATGGACAG CCATCGTCTG 
ACCTACTTTG GCACACTATG CAGCATCCTC TTCAATGTCG TCCTGCTGAG CACTTTTGTT 
GTCCCTTTTG TGGAGGAAAA ACAGAACGGA CTCAAGGAGT TTCTAAATCT AGTCACTCCA 
ATGAGTTTCC TGAATGGCCT CACCTTCTTT TTCATTCGCT TTATCTGCTA TGCAGCGCTC 
CTTCTATTTG TCCTTATCGC CGCCTACTTT TACGAGGCTC TGGGCCTGAT TGGCTTCGCT 
TACATCGCTG TTTTGTATCT GCTTTACATA TTGGCCACTA TGTCGTACGC GTACTTAATC 
TCCGTGTGCT TTCACTCTGT TTTCTACGCG AAAATTGGCG GCCTGGTGAT GCTGATCATT 
CCCTACGTGT TCAGTTTTGT CCGCAGCTGG GTCACCTCTG TGGCCTTTTT CCTGTTTAGC 
ACCAATGCCT TCCTTGAGGG CTTGGATGTG TTCCAGACCT TCTCCAACAA ACATCGCCAC 
TTTGGGGCAG CGGATCTTTT CCGTGAGATC AAAGATGACT CCACCCGCAT GTTCTGGATC 
TACTGTTCAC TCATTGCTCA ATCCATCCTC TACGCTCTGC TTTACAACTA TATGACCTGT 
GTTTTCCCCG GTCCTGGGGG ACTCAAGCGT CCTTTTCTGT TCTTCCTGGA TCCCAATACG 
TACAAGAAGC GACAACGCAA TGAGTACACA ACTGCACCGC GTGGAACCCA TGCCATCATC 
ATATCGGAAC TGTGCAAGAA GTTCCAGACA AGTAAACGCG AAACGCTTAT TTCGGACAAT 
CTAAATATGA CGATTAACAA CAAGGAGATC ACTGTTCTCT TGGGCCACAA CGGAGCCGGA 
AAGACAACGA TGATGAACAT GATTATGGGT CTGGTCCCGA AGGATGCGGG CAAGATTGTT 
GTCTGTAGCG AGCGCGATGT GGCCTCGTAT CGCCACCTGA TTGGGTTCTG TCCCCAGCAC 
AGTGTTTTCA TGAGCTACAT GACGTGTCAT CAGCATTTGG AGTTCTTTGC CCAGCTGCGC 
GGGTCACGCC GCTCGGATGC GTGTCTTTGG GCTGATACCC AGCTCAAAAA ACTGGGCCTC 
AAAGAGAAAC GCGACGAGTA TGGGCGCAAT TTGTCAGGGG GAATGAAGAG GCGCCTGTCG 
CTGGGCATTG CCATTGCAGG AAACACAAAA ATTGTGATTC TGGACGAGCC CTCGTCTGGA 
CTGGACATCG AGTCCCGACG CCAGTTGTGG GACATCCTGT TGAATCTGCG CAAGGAGAAG 
GCCATTCTGG TCACCACCCA CTACATGGAG GAGGCCGAAG TGCTGGGTGA CACCATCTGC 
ATCTTGGCCA ACGGAAAGCT GCAGAGCACT GGCTCCCCTC TGGAACTGAA GCGTAGATTG 
GGCATCGGGT ACCGCCTAAA GCTGGAAGTC ACCGATTTCA CATTCCGCGA ACGTGAAATA 
ATGAAAATTA TACTCGATTA CGTTCCGACT GCCCGCGTGC TGAATGTGGT GAAGCCGACT 
GTCTACATTT GCCTGCCGTA TGCGTACAAG ATGTGTTTCT CGGAAATGCT GCTCAAACTG 
GAGTCGAGGA CAAGTGAGCT GGAAATAAGT ACCATTTCTA TGACGGATAC TTCACTAGAA 
GATGTTTTTC TAAGATGTGC GGGAGAGCAG GATCAGGTGG ACACTCCAGA CGGCTCTCGA 
AACGATGCTC CCCTGCTGAC CGCGCTCTAC AAGCGACTAC CCAGCCAGCA CACTCCGCCC 
AGCACGTGGC AGCTGTGGCT GGCGGTGTTC TACAAGAAGT GGACGTTCCT TGCCAACGAG 
TGGTTATATG CTGTCATAAT GCTGTCCCTT CCCGTTGTGT GCGTCTCTGG AGCCATTCTC 
GCTATGCACG CCATGTCTGT TGTGAAGAAT GAGGCGGCCC TACCGCTCAA GCTCAGTCAG 
ATGGGCTCTG GAGTAGTTTA TATATACAAT CCGACGGGCC ATCATGCTCA GGTTGAGCAA 
CAGCTGCGGC AGCAGATCGA ACTTAGTGGA CTCACGGCCA AAACGTTGCA GCTACGCGGA 
AACAGTGTGA AAAATGAGCT CCTCGAATTG CAACGCGACA ACTTGGCGGA TTTCCTGGAA 
CAGGTGGTGG GCGCTATCGA CATGTACGGC GGGGGCCGGG GCACTGGAGG AACTCCGCAG 
ATAGAAATCT TTTACGCGGG CAATCGCTAC CACAGCTCAG TGGAGCTGAT CAACTTTCTG 
GACTCCAGCA TGCTGAAGAT GGTCAGCCCA GAGTCGGACA TTGAAACCAC GTATGTGCCG 
ATTCGACGCT TCATCAGCGA CATCAGTCCC ACGCGATTGG AGTACTATGT GGTGATCGTG 
TCCGTGGGAA TGTTCTTCTG CATGTTCTAC TTCATTTCGC TACCCTTCCG AGAGCACGAC 
AACGGCTTCC GCCAGCTGCA GACCATGTCA CGCTACACCT TCTGGCTGTC CACCTTTGTG 
TTCGACGTCC TGCTGCTGGT CCTCCTCTGT GTGGCGCTTT TTGTGCTTCA GAACTGGCTA 
ATGCCTTCGG AGCTCTACTC CACCTCAGAA CTGAGAATCA TTGTTATGAG CATATTCTTC 
TACGGCTGCA GCTACCTGCC CATTCTGTAC TCGCTGGCCA ATAACTTCAA GTCCATATCG 
ACGATATCCA CCTACCTGCT GCTGATGCTG ATTGTGTCCG CAATTGCTCC TCTGATCACC 
TCCAACAATG CGCAGTCCAT AAAACTGCAC GAGACGAAGA TAGCCTTCCT CTGCTTCCTG 
CCCGACTTCA ATCTGAACCA CCAGCTGCGC ATCATCAATG AGAATTTCAT TGCTAACCGA 
CGCTCTGTAC CCACCGCCCA TTTGCTTTCG ACGGATACGT TCTTTGTCTA TGCCATGGCC 
GTCTGCGTGC TGGTCTTTTC ATTCTTCACC ATCGTTTTGG AGCACAAGTA CCTCCGCCAA 
CGGGTCCACG ATGCCATCTG GATGAACAGG TGCCTGGTCA CCTTGAAGAA GAACAGCTCG 
ACGGGCACCA GTTCTAGCAC CCAGCTCACG GTCGTTGACG ACTGTGCCAA CGAAGAGCGT 
CGAGTGGAGG CGCTCATGAT CGATCCGACG CAGGAGTGTC CCTTGATCGT CAGCAAAGTG 
CGCAAGCGGT ACCACAAGAA AGTGGTGGTG GATGGAATCA GTTTTGCCGC TTCTAGAGGC 
GAATGCTTTG GCCTCTTAGG CATCAATGGA GCCGGAAAGA CGAGCACATT CGAAATGATT 
GCCGCCAATC TGCCTCTGGA CGGAGGGAGC ATACGAATCG ATGGCATCGA TATTCGAAAG 
GACGAAGTAG GCTATCGCCA TCGTTTCGGC TACTGCCCCC AGTACGATGC CCTGAATAAG 
TTTATGACCG CCGAACAATG TCTCCACTAT ATGGCCCTCC TGCGGGGACT GAGCACCACC 
AGCGAAGGAC CGAGTAGCGT GACGGAAAAC GTGAAATACT GGCTCGAAAA AATGCACCTG 
ACCAAGTATC AACAGGTCCA GGTGCGACAT TATTCGGGGG GGACGAAGCG CAAACTGATG 
GCTGCCATGG CGATGATCGG GGCCCCGACA CTGGTTCTCC TAGACGAGCC CACGACTGGC 
GTGGATCCCA TTTCCAGACG CTTTCTGTGG GAGTGCATCA AGGACTTTCA GGGCCAGGAG 
CGCACTGTGG TGCTTACTTC ACACAGCATG GACGAGTGTG AGGAGCTGTG TAATCGCCTG 
GCCATCATGG CCAATGGGAA ATTCAAGTGC CTGAATACCA TCTGCGCCTT GAAGAGACTG 
AGCGGCTTCA CCATAAAACT TAAGATGAAA CTAGACACGG AAACGGAGCA GAACGTGCAC 
ACTATCACAA ATACCCTGAA GGCGCAGTTC GAGGGTCTGG AGTTGCGCGA GAATCATGCC 
GGAACCCTAA CCTACTTTGT TAGCACCCAG GAGAACTTGG TCTATTGGTC GAGGGTGTTT 
AGGATAACCG AGGACCATCT GACCGACAGA TTGGGCCACA TCGTGGCGGA CTACTCGCTG 
AATGAGTGCA CTTTGGAAGA TATTTTTCTC AAATTCGACA AAAAATCGAA GACCCCACCC 
TCATCGCAAA CATCGTCGCG CAACTCCCCA GTCCAACACA ACCCTGACGC GTCCGACTTT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA16429 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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