Dpse\GA21425 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA21425
Entrez Gene ID	4814908
Full Name	GA21425 gene product from transcript GA21425-RA
Synonyms	GA21425,dpse_GLEANR_602
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001354541.3	XP_001354577.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA21425 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA21425 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe09558	XM_001354541.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA21425), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA21425 ( XM_001354541.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001354577.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA21425), partial mRNA.

Price & Availability↑

CloneID	ODe09558
Clone ID Related Accession (Same CDS sequence)	XM_001354541.3
Accession Version	XM_001354541.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589960). On Dec 22, 2015 this sequence version replaced XM_001354541.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGGTGGTCA AGCTGCTCTT GGCCAACCTC TGGTTCTGTC TCTCGGTCTG GGACTGGTGC 
CCCTGTCAGG CCTATGATTT TGGCTACCAT CCCAGCGCCG AGGAGTTCGA TAGTCTGTGG 
CATCGCGAGC TGCTCGCCAA CGATCGCCGG CGCATGGAGC AGCGCTTTGT CCCGCGGTAT 
CAGCATGCAG AGACCTCGAT CCGCTTCGAG CCACCAGAGC TGGATTTCCA GTTGTTTAGC 
CAGGAGGAGG ATGCGGATCA GCAGCAGCCA TATGTGTTCG AGTCCGTGGA GGAGGCACCC 
ACCAATACGC AACCCGCACC CGATCTGATG CATGTGCAGA GTCAGCTGCT GGAGATTAAG 
GATCCCACAC CTCAGCGGCC ATTCGTCAAT CACCATCGTC CAGCGAATGG CTCCTCCGCT 
CAAGCCAGTT TCCCCATCTT CTTTACCAAC CCTGCCACAG GCATTGTCTA TGCCATCAGC 
CAAGTGGGAG AGGGTGCCCA AGGTGTCCAG GGATCTCAAC CCCGCAATGA CAGCGGCGGC 
GCCATAGCCA TCTATGTGAC CAAGGCACAG TATGATGCGG ATATCCAGGC ATTGCGACAA 
CACTACGAGG AGCAGTGCCA GCAGCAGCAG CAGCAGCAGC AAACCATTCC CCCGGGCACC 
GTGCTCTCCA CGCCGAACCT GAAGCCGAGC CCCCAACAAG GAGGAGTGAC TGCTCGGCCA 
CAGATCATAC GGCTGCAGAA GCCCAAAAAG AGGCCGGGTG GTGTCACCAA CAAGCTGCCG 
AAGCCACAGC ATCGACCGCC ACCGGTGATG GTGACCAGTG GACCCATAAC CACCATCAAC 
GATCAGGTGT TTAAGATTCA CCACAAGAGT AGCAGCACCA CCACCACACG GAGACCCAAG 
ACATCCACAA CCAGGCGAAA GAAGCGCAAG AAGAAGAAGC CGAATCCCGT GAAGGCAATT 
AAACGCCGGC CTGGACACAA GCGGAGGAGT AACAGCACCA CAGAGCGACC CGCAGGTCCA 
CCACCCATTA TCCTGGGCAC GCGACCGAGC AGCAGCAGCA GCACAACAAC CACGACGACA 
ACGAGACCGC CGCCTGGATC CACACAGCTG GAGAAGCCCC ATATGCCGCA GGCCACCACA 
TCGACGGGGA ATATTTTCAG TCAGCATGCA TATGTGATTA ACCAGGGATA TCATCCCCAG 
CATCCTCATT CCCATGTGAG ACGTCGTCGG GGACTAGAAG CCGCAGCAGC AGGAGTAGGA 
GAAGGTGCGG TAGAAGCAGC AGGAGGATCG TCAGCAGCAG TAGGAAGAGG AGCAGTTGTT 
AGAGGATCGA CAGTGGAAAT GCCTCTAATA AAAAGAGCGG CAGTAGGAGG ATCAGCTGCA 
GAAAGATCTG ACGTAAAAGT ATTACCCATT CGAGGATCAC CAACAAAAGG ATCATCGGTA 
GTGAGAGCAA AAGGAGGAGA GGCCGTCGGG TTAGGAGGAT CAGTTGGAAG AGGATCAGCA 
GCAGCAACAG CAGCAACAGC AGCAAAAGGA TCATCTTCAG CAGTGGAAAA AAAAGCACTG 
GTCAGAGGAT CGACAGTAAA AGGACCACTT AAGTCAGCAG TCCAACGGGC AGCCACCAGA 
TCATCATCCA CAGTCGTGAG AGGAGGATCA GCCGGTGCTC GAAGAAGGGG TGGTGGATCT 
GGAAGCCCAA GGCCAAGGGG ACCCAAGGGC AGCAGTAAAA GCAAATCCAA GCCAAGGCCG 
CGAACAACCC CAGAAGTTGG CGGTGATCGT GCGGCAGGCG GCGCTGGTGT GGCCACTGGA 
GTGCTGCACC TGCCACACGC TGTCCACCTG CTGCACAGGA ATCACAGTAC AGACTCGAAG 
GAGAATCATC ATTCTCAGCC AGATCAGATC AAAATCCAAC CAAAGAAAAA GAAAAAATCC 
AAGGCAAAGG CAAAGGAAAA GGAGAGGAGA CGCGGTCGTC CCGAGGGCTC GAACTTTGAT 
ATATTCGAGT TAATTTCTGG CGGGGATTAC GATGACGATA CCGAAGAGGA TGATGAGTAC 
TATTACGAAG ATGAGAATGA GAATGGAGAT GGTGACGAAA AGGAGCCTCA GGCGGGACAA 
GCTACCAGCA GTAGCAGCCC CGAGGACCCC AAGGAGGACA GCAGCTCGGC GGAGGAGGGA 
GCCGACTTTG GGGCCATGGA GGAGTTGGAG ACGACGACCC TCTCACCAAC GAGCACTGAG 
CTGCTGGAGG GGGACCAGTT TTCCAGCACG AAGAACAGTA TGGCCAAGAA GCGTATACGC 
ATCCGTAGAC GACCGCCCGC TTCCTCCTCC TCCGAGGAGG ACGACAACGA TGATGATTCG 
GGTGGCATCG GTGGCTTCTT TCGCATGATC TTCTACCCCG TTCAGGTGGC CATGTCCCGT 
CTGATGGACT TGGGCTTTGG TGGTCCATCA TCGTCCAACG ATGAGGACGA GGAGGAGGTC 
CACAAGGTGA ACCCCACAAA GCCCCCCAAG TATCCCAGCT ACACGCTCTA CCATAGCGCC 
CACAGCAACG AGGCCTATGC CCAGCCCGAG GACGACGAGG ATGAGGGCGA TGATGACGAC 
GACTATGATG GCAGCAGCAC CCTGGGCAGT TGGTTCAGCT CCTGGTTCGG ACCTGGACGA 
CGCACTAAGA AGATTGGTAG CACCACCACA GTGGTGCCAC TGCCAGCGCC CACCCAAGAG 
CCAGCCGGTT GGCTGGACTC ATGGTTTGGC TTTGGCAAGA CGACAACAAC AACGCCCACG 
GATTCCGACG AAGATGACTA TGACAAATGG TTTTCCAGCT GGTTCGATGC GAAGCCCAAA 
AGGAAAGTGC GTCGCCGCAG CACAACCTCA ACGACCACCA CATCGACGAC AACCAGCCAA 
CCGTCTGCTG CCGCAGCCGC TGCCGCTGCC GCTCAGGTGC CCATACTGAC AATTGTGGAT 
CCGTTGAGAA ATCCCCAGAA CTGGATTGGG ATACTCGCCC ATCACATTGT CAACTCCACG 
GGCAACGCGA TGACGACCAG GATGACGACC AAAGGGACCA CCACCGTCAA GCCGGATATA 
CCGCGCCGGA TAAGCTATGA CAAGTATCAG ATCTGGCGAC TCAAGCCGCA GGATGAGGCA 
CAGGTCCGCG CACTGGAGGA GTTCAAGAAG GGCGAGGATG GAGTCAAGAT GCACTGGCTC 
AAGGGCCCCA GTCTCAGAGG TCTCACCGAT GTCCTAGTGC CGCCCAAGAT GCTGGTGGAT 
TTCCAGGGAA CTCTCAACTA CGAGAGCATT GCCCATGAAG TTCTCATCTT CGATGTGGGC 
ATGGCCATTG CCTACGAGCA GTCGAAGGAG GACTATCTGC ACACCACACC CGTCTCACTG 
CAGCGTCCGT CCAGGCCCAA GCCCAAGCCC ACGGCCGTCG CAAAGCCTGG AATGACCTGG 
AATCGCTATC ACACTCACGA CGAGATTGTC CAGTACCTTG AAACGATGCG TATGAGGCAT 
CCACAGCTCG TCGAGCTCAT CCACATAGGA CGCTCCTACG AGGGCCGTCC CCTGATCGTT 
GTCAAGATCG AGTCCAAGCA ATCTGCTTCA GCAGCCAATG CCTCGGCCTC GGGCTACAAG 
AGACCCAAGC GTAAGAGGAA ATCTGGTCAG GCGAATGCCG TCTTTGTGGA GGCTGGAGCC 
CATGGTCTGG CATGGATTGG ACCCGCGACG GCCACCTGGA TGATCTCTGA GCTGCTGCGA 
CTGATGAAAA CCAACAAGAG CAACGAAGAA CTAGAGCTCA TACGGAACAC CACCTGGTAC 
ATAATGCCCG TCCTGAATCC CGATGGATAT GCGTACAGTC ACGAGTACGA TCGGTTCTGG 
AAGAAATCAC GCTCCCAGCA TATGGCCCGA GCGCCGGGCG GACTCCTCGA CTCGGCCATG 
ACATGGCTGC AACAGAAACG CGACCCGGAC AAGGTATGCC ACGGCGTCGA TCTTGACCGG 
AACTGGCTGT ACCACTGGGG CAAGCGGGGC AGCTCCAAGG CGCCGTGCAA TGAGTTCTAT 
GCGGGGCCCG CCCCCTTCTC GGAACCAGAG ACGAAGGCCG TCTCCGACTT TCTCATGGAT 
CAACGCACAC AGATAAAGCT CTACATCTCC CTGCAGGCGT ATGGCCAAGT GGTTTCCTAT 
CCGGTTAAGG CCAATTCCAC ATTCAATTCG GAGCTCTTGG ATGACTTCCT GGACGTGGCC 
ATGGTGGGCA CCGATGGCCT GCGAAAGAAG GGCAGCAAGT CGCGCTACAA AGTGGATGCC 
TCCAACGATC TGATTGAGCA GCGCTCTGGT TGCGCCGACG CCTTTGCCGC CTACGAGATC 
GGCATACCCT TCAGCTATAC GATCCAGCTG GCGGATAATG GGGTCCATGG CTATCTGCTG 
CCCAGCAGCG CCATTGAGCC GACAGCTCGG GATGCCTTCG AGATCGTCAG CGGGATGCTG 
GACTATATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001354577.2
CDS	1..4452
Translation	MVVKLLLANLWFCLSVWDWCPCQAYDFGYHPSAEEFDSLWHRELLANDRRRMEQRFVPRYQHAETSIRFEPPELDFQLFSQEEDADQQQPYVFESVEEAPTNTQPAPDLMHVQSQLLEIKDPTPQRPFVNHHRPANGSSAQASFPIFFTNPATGIVYAISQVGEGAQGVQGSQPRNDSGGAIAIYVTKAQYDADIQALRQHYEEQCQQQQQQQQTIPPGTVLSTPNLKPSPQQGGVTARPQIIRLQKPKKRPGGVTNKLPKPQHRPPPVMVTSGPITTINDQVFKIHHKSSSTTTTRRPKTSTTRRKKRKKKKPNPVKAIKRRPGHKRRSNSTTERPAGPPPIILGTRPSSSSSTTTTTTTRPPPGSTQLEKPHMPQATTSTGNIFSQHAYVINQGYHPQHPHSHVRRRRGLEAAAAGVGEGAVEAAGGSSAAVGRGAVVRGSTVEMPLIKRAAVGGSAAERSDVKVLPIRGSPTKGSSVVRAKGGEAVGLGGSVGRGSAAATAATAAKGSSSAVEKKALVRGSTVKGPLKSAVQRAATRSSSTVVRGGSAGARRRGGGSGSPRPRGPKGSSKSKSKPRPRTTPEVGGDRAAGGAGVATGVLHLPHAVHLLHRNHSTDSKENHHSQPDQIKIQPKKKKKSKAKAKEKERRRGRPEGSNFDIFELISGGDYDDDTEEDDEYYYEDENENGDGDEKEPQAGQATSSSSPEDPKEDSSSAEEGADFGAMEELETTTLSPTSTELLEGDQFSSTKNSMAKKRIRIRRRPPASSSSEEDDNDDDSGGIGGFFRMIFYPVQVAMSRLMDLGFGGPSSSNDEDEEEVHKVNPTKPPKYPSYTLYHSAHSNEAYAQPEDDEDEGDDDDDYDGSSTLGSWFSSWFGPGRRTKKIGSTTTVVPLPAPTQEPAGWLDSWFGFGKTTTTTPTDSDEDDYDKWFSSWFDAKPKRKVRRRSTTSTTTTSTTTSQPSAAAAAAAAAQVPILTIVDPLRNPQNWIGILAHHIVNSTGNAMTTRMTTKGTTTVKPDIPRRISYDKYQIWRLKPQDEAQVRALEEFKKGEDGVKMHWLKGPSLRGLTDVLVPPKMLVDFQGTLNYESIAHEVLIFDVGMAIAYEQSKEDYLHTTPVSLQRPSRPKPKPTAVAKPGMTWNRYHTHDEIVQYLETMRMRHPQLVELIHIGRSYEGRPLIVVKIESKQSASAANASASGYKRPKRKRKSGQANAVFVEAGAHGLAWIGPATATWMISELLRLMKTNKSNEELELIRNTTWYIMPVLNPDGYAYSHEYDRFWKKSRSQHMARAPGGLLDSAMTWLQQKRDPDKVCHGVDLDRNWLYHWGKRGSSKAPCNEFYAGPAPFSEPETKAVSDFLMDQRTQIKLYISLQAYGQVVSYPVKANSTFNSELLDDFLDVAMVGTDGLRKKGSKSRYKVDASNDLIEQRSGCADAFAAYEIGIPFSYTIQLADNGVHGYLLPSSAIEPTARDAFEIVSGMLDYI

Target ORF information:

RefSeq Version	XM_001354541.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA21425), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001354541.3

ORF Insert Sequence:

ATGGTGGTCA AGCTGCTCTT GGCCAACCTC TGGTTCTGTC TCTCGGTCTG GGACTGGTGC 
CCCTGTCAGG CCTATGATTT TGGCTACCAT CCCAGCGCCG AGGAGTTCGA TAGTCTGTGG 
CATCGCGAGC TGCTCGCCAA CGATCGCCGG CGCATGGAGC AGCGCTTTGT CCCGCGGTAT 
CAGCATGCAG AGACCTCGAT CCGCTTCGAG CCACCAGAGC TGGATTTCCA GTTGTTTAGC 
CAGGAGGAGG ATGCGGATCA GCAGCAGCCA TATGTGTTCG AGTCCGTGGA GGAGGCACCC 
ACCAATACGC AACCCGCACC CGATCTGATG CATGTGCAGA GTCAGCTGCT GGAGATTAAG 
GATCCCACAC CTCAGCGGCC ATTCGTCAAT CACCATCGTC CAGCGAATGG CTCCTCCGCT 
CAAGCCAGTT TCCCCATCTT CTTTACCAAC CCTGCCACAG GCATTGTCTA TGCCATCAGC 
CAAGTGGGAG AGGGTGCCCA AGGTGTCCAG GGATCTCAAC CCCGCAATGA CAGCGGCGGC 
GCCATAGCCA TCTATGTGAC CAAGGCACAG TATGATGCGG ATATCCAGGC ATTGCGACAA 
CACTACGAGG AGCAGTGCCA GCAGCAGCAG CAGCAGCAGC AAACCATTCC CCCGGGCACC 
GTGCTCTCCA CGCCGAACCT GAAGCCGAGC CCCCAACAAG GAGGAGTGAC TGCTCGGCCA 
CAGATCATAC GGCTGCAGAA GCCCAAAAAG AGGCCGGGTG GTGTCACCAA CAAGCTGCCG 
AAGCCACAGC ATCGACCGCC ACCGGTGATG GTGACCAGTG GACCCATAAC CACCATCAAC 
GATCAGGTGT TTAAGATTCA CCACAAGAGT AGCAGCACCA CCACCACACG GAGACCCAAG 
ACATCCACAA CCAGGCGAAA GAAGCGCAAG AAGAAGAAGC CGAATCCCGT GAAGGCAATT 
AAACGCCGGC CTGGACACAA GCGGAGGAGT AACAGCACCA CAGAGCGACC CGCAGGTCCA 
CCACCCATTA TCCTGGGCAC GCGACCGAGC AGCAGCAGCA GCACAACAAC CACGACGACA 
ACGAGACCGC CGCCTGGATC CACACAGCTG GAGAAGCCCC ATATGCCGCA GGCCACCACA 
TCGACGGGGA ATATTTTCAG TCAGCATGCA TATGTGATTA ACCAGGGATA TCATCCCCAG 
CATCCTCATT CCCATGTGAG ACGTCGTCGG GGACTAGAAG CCGCAGCAGC AGGAGTAGGA 
GAAGGTGCGG TAGAAGCAGC AGGAGGATCG TCAGCAGCAG TAGGAAGAGG AGCAGTTGTT 
AGAGGATCGA CAGTGGAAAT GCCTCTAATA AAAAGAGCGG CAGTAGGAGG ATCAGCTGCA 
GAAAGATCTG ACGTAAAAGT ATTACCCATT CGAGGATCAC CAACAAAAGG ATCATCGGTA 
GTGAGAGCAA AAGGAGGAGA GGCCGTCGGG TTAGGAGGAT CAGTTGGAAG AGGATCAGCA 
GCAGCAACAG CAGCAACAGC AGCAAAAGGA TCATCTTCAG CAGTGGAAAA AAAAGCACTG 
GTCAGAGGAT CGACAGTAAA AGGACCACTT AAGTCAGCAG TCCAACGGGC AGCCACCAGA 
TCATCATCCA CAGTCGTGAG AGGAGGATCA GCCGGTGCTC GAAGAAGGGG TGGTGGATCT 
GGAAGCCCAA GGCCAAGGGG ACCCAAGGGC AGCAGTAAAA GCAAATCCAA GCCAAGGCCG 
CGAACAACCC CAGAAGTTGG CGGTGATCGT GCGGCAGGCG GCGCTGGTGT GGCCACTGGA 
GTGCTGCACC TGCCACACGC TGTCCACCTG CTGCACAGGA ATCACAGTAC AGACTCGAAG 
GAGAATCATC ATTCTCAGCC AGATCAGATC AAAATCCAAC CAAAGAAAAA GAAAAAATCC 
AAGGCAAAGG CAAAGGAAAA GGAGAGGAGA CGCGGTCGTC CCGAGGGCTC GAACTTTGAT 
ATATTCGAGT TAATTTCTGG CGGGGATTAC GATGACGATA CCGAAGAGGA TGATGAGTAC 
TATTACGAAG ATGAGAATGA GAATGGAGAT GGTGACGAAA AGGAGCCTCA GGCGGGACAA 
GCTACCAGCA GTAGCAGCCC CGAGGACCCC AAGGAGGACA GCAGCTCGGC GGAGGAGGGA 
GCCGACTTTG GGGCCATGGA GGAGTTGGAG ACGACGACCC TCTCACCAAC GAGCACTGAG 
CTGCTGGAGG GGGACCAGTT TTCCAGCACG AAGAACAGTA TGGCCAAGAA GCGTATACGC 
ATCCGTAGAC GACCGCCCGC TTCCTCCTCC TCCGAGGAGG ACGACAACGA TGATGATTCG 
GGTGGCATCG GTGGCTTCTT TCGCATGATC TTCTACCCCG TTCAGGTGGC CATGTCCCGT 
CTGATGGACT TGGGCTTTGG TGGTCCATCA TCGTCCAACG ATGAGGACGA GGAGGAGGTC 
CACAAGGTGA ACCCCACAAA GCCCCCCAAG TATCCCAGCT ACACGCTCTA CCATAGCGCC 
CACAGCAACG AGGCCTATGC CCAGCCCGAG GACGACGAGG ATGAGGGCGA TGATGACGAC 
GACTATGATG GCAGCAGCAC CCTGGGCAGT TGGTTCAGCT CCTGGTTCGG ACCTGGACGA 
CGCACTAAGA AGATTGGTAG CACCACCACA GTGGTGCCAC TGCCAGCGCC CACCCAAGAG 
CCAGCCGGTT GGCTGGACTC ATGGTTTGGC TTTGGCAAGA CGACAACAAC AACGCCCACG 
GATTCCGACG AAGATGACTA TGACAAATGG TTTTCCAGCT GGTTCGATGC GAAGCCCAAA 
AGGAAAGTGC GTCGCCGCAG CACAACCTCA ACGACCACCA CATCGACGAC AACCAGCCAA 
CCGTCTGCTG CCGCAGCCGC TGCCGCTGCC GCTCAGGTGC CCATACTGAC AATTGTGGAT 
CCGTTGAGAA ATCCCCAGAA CTGGATTGGG ATACTCGCCC ATCACATTGT CAACTCCACG 
GGCAACGCGA TGACGACCAG GATGACGACC AAAGGGACCA CCACCGTCAA GCCGGATATA 
CCGCGCCGGA TAAGCTATGA CAAGTATCAG ATCTGGCGAC TCAAGCCGCA GGATGAGGCA 
CAGGTCCGCG CACTGGAGGA GTTCAAGAAG GGCGAGGATG GAGTCAAGAT GCACTGGCTC 
AAGGGCCCCA GTCTCAGAGG TCTCACCGAT GTCCTAGTGC CGCCCAAGAT GCTGGTGGAT 
TTCCAGGGAA CTCTCAACTA CGAGAGCATT GCCCATGAAG TTCTCATCTT CGATGTGGGC 
ATGGCCATTG CCTACGAGCA GTCGAAGGAG GACTATCTGC ACACCACACC CGTCTCACTG 
CAGCGTCCGT CCAGGCCCAA GCCCAAGCCC ACGGCCGTCG CAAAGCCTGG AATGACCTGG 
AATCGCTATC ACACTCACGA CGAGATTGTC CAGTACCTTG AAACGATGCG TATGAGGCAT 
CCACAGCTCG TCGAGCTCAT CCACATAGGA CGCTCCTACG AGGGCCGTCC CCTGATCGTT 
GTCAAGATCG AGTCCAAGCA ATCTGCTTCA GCAGCCAATG CCTCGGCCTC GGGCTACAAG 
AGACCCAAGC GTAAGAGGAA ATCTGGTCAG GCGAATGCCG TCTTTGTGGA GGCTGGAGCC 
CATGGTCTGG CATGGATTGG ACCCGCGACG GCCACCTGGA TGATCTCTGA GCTGCTGCGA 
CTGATGAAAA CCAACAAGAG CAACGAAGAA CTAGAGCTCA TACGGAACAC CACCTGGTAC 
ATAATGCCCG TCCTGAATCC CGATGGATAT GCGTACAGTC ACGAGTACGA TCGGTTCTGG 
AAGAAATCAC GCTCCCAGCA TATGGCCCGA GCGCCGGGCG GACTCCTCGA CTCGGCCATG 
ACATGGCTGC AACAGAAACG CGACCCGGAC AAGGTATGCC ACGGCGTCGA TCTTGACCGG 
AACTGGCTGT ACCACTGGGG CAAGCGGGGC AGCTCCAAGG CGCCGTGCAA TGAGTTCTAT 
GCGGGGCCCG CCCCCTTCTC GGAACCAGAG ACGAAGGCCG TCTCCGACTT TCTCATGGAT 
CAACGCACAC AGATAAAGCT CTACATCTCC CTGCAGGCGT ATGGCCAAGT GGTTTCCTAT 
CCGGTTAAGG CCAATTCCAC ATTCAATTCG GAGCTCTTGG ATGACTTCCT GGACGTGGCC 
ATGGTGGGCA CCGATGGCCT GCGAAAGAAG GGCAGCAAGT CGCGCTACAA AGTGGATGCC 
TCCAACGATC TGATTGAGCA GCGCTCTGGT TGCGCCGACG CCTTTGCCGC CTACGAGATC 
GGCATACCCT TCAGCTATAC GATCCAGCTG GCGGATAATG GGGTCCATGG CTATCTGCTG 
CCCAGCAGCG CCATTGAGCC GACAGCTCGG GATGCCTTCG AGATCGTCAG CGGGATGCTG 
GACTATATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA21425 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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