Dpse\GA18539 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA18539
Entrez Gene ID	4814208
Full Name	GA18539 gene product from transcript GA18539-RC
Synonyms	GA18539,dpse_GLEANR_7117
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001354293.3	XP_001354329.3	uncharacterized protein, isoform C
XM_015186908.1	XP_015042394.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA18539 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA18539 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe25407	XM_001354293.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA18539), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODe25423	XM_015186908.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA18539), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA18539 ( XM_001354293.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001354329.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA18539), mRNA.

Price & Availability↑

CloneID	ODe25407
Clone ID Related Accession (Same CDS sequence)	XM_001354293.3
Accession Version	XM_001354293.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589958). On Dec 22, 2015 this sequence version replaced XM_001354293.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACAGG CTCCGCGACC CGTGCCGGCT CCGCGGCGGC TCTGCACGGA GCTACGTCCC 
GCCAACTATG AGAACGTCGA AATCAAGCCT AGCAACAACA ATAACAACAA CAATACTATC 
ATCAGTTTCA AGAAGCTTAA CATCAACGCG GAGAACTTGC ACGGCAATTG TGCGGAGAAG 
AAGCTGCCGG CAAGTGGGCA GCACAAGCTG CAAGTGCTGC AGGAGAACAG CGCCGAGCAG 
CAGCAGCCCA TCTATGGTCC CGACGCCAAC GAGAATGTCC CGACAGAACC GATCTATGCC 
GCGCCCCGAC CAGCGCCGAG ACAGCGACTC ACCACGGAGT CGCTCCCGCA GGAGCCGCAA 
GTCGAACTTG ATGTGGACGC GGTTCCTCTG AGAGCTGTGC GCACAGCTCC CCAGGTGCCT 
CCGAAGCCGC CCAGCCTGGA ACAGCGCTTG TCGATATGCA GCGAGATGGA GCAGCAGGGG 
GATGCTACGG CCTCCTCTCT CTACGGCTCG AACGCCTCGC TGGACTGCTG CTCGCAGGGC 
TCGCAGAGCG GCAAGTTCAA GTCGCAGTCA CCCGGGTACG TAGACGACGT TGATCAGCGG 
GGTCAGCCAT TTCCGGAAGA TTCCTCTGAC CCGGAAACAC CTCCGCCAAC GCCAACACCA 
CCACCACCTT CGACATCTTC ATCGACGTCG CTCGATCAGT CCTCCAGCTG TGATCAAGAA 
CAAGACCAAG ATCGAGAAGA TGACAAGAAT CTTCTGCGAT CCTTGGGCAC CACCTCGAAA 
CTGCTGGGCG AGGCCATTGG CGAACGGATC ACGTTCAAGG CCAAGGGTGC CAAGAAGCGT 
CTGGACAGGA ACCTAAGGAG CTCCACGGAG GCGATCAGCA ATCTCAGCGC TGACGCTGGC 
AGCCGTCTGA AGAATGCCAG CAGGCGATTC GGGTCGAACT TCACCTTGGG GGGCAGAGGA 
GGCCATGGGG ACAGAGAAGC GGAGGGTGGA GAAAGCTCCG TGGGAGAGTT TGACCTTGAG 
CGACGGCAGA CCATGCCCAA CACGGATGTC TTCAATGCGA TACAGTTCTC CAGTCCCCTG 
AACCGGAATG TCGTCGGCGG AGGATTCACC GATCTCCGGG ATGAGGCAAA GGTCACTGCC 
AAGGTCATAG TTAAGGATCA AGAGGTGCCA GTTGGGGGGG ACGGTGCCGG GGATGATGAC 
GGCTGTTATG AGGTGCCCAA GGTGCAGGGC AAGCCCCCCA GCTACGATGA GGCACTGAGG 
GCACGTCCCT CCCCACCTCC CCCGCTGGCC AAGAATCTCT CTCAGGAGCA CGTCCAGCTT 
CGGACGCAGC AGCGTCCCCC GATCCGCGGA GATGACGCAA CGACGTCGTC TCCGCCATCG 
TCCCTGCCAA TGCCCGCGTT CCCTCCGCCG CGCCTCTCCC AACAGCCAGA GCGGTTCCGG 
CTGGACGCAC TGCAGCCGCC CGTGCGGCAG AAGCGTCGCA AAAACTACGA GCACATCGAG 
TTGCGACGTC CCCCCGTGGA CTCCGCCGAG CTGAAGGAAC TGAATCAGGC AGCGGCGGTA 
GCGGAGCGAG AGGAATCGCT GCTAATCTGC GCCCAGATTG CCGCCGAAGA GGCGAGGGCC 
CCAAAGCCAG AGCGTAGCGA TTCCTGGCAG TTCTACGGCG AGGATGTCGC TTCGCAGCAG 
GGAGAAGCGG TGCAGGAAGA AGACTCCTCC CGGGAACCTG TCTATGCCAA CCAGGAAGCC 
ACCTACGGAA AGCTCTTCGA GATGGCCACC GCGGGGAGCA GTCGCGGCTG CAACTTGTTG 
ACCCCCAACC AGCAGCAGCA GCAGAACCAG CAGCAGCAAG AGCTGGCCTG CATCAGCGAA 
GATGGGGAGC TGGACAGCAA CGACGGAGCC GTCGGTGGAT GGTGCGCGCC CCAGGAGCTG 
ATCGAGGAGT TCGATCCATT AATGAGCAGC AAGTGCTCGA CGCTGGAGCG TTCCAACAAG 
AGCAACGAGC TGCTACTGCT CGAGCACCTA CTGGAGGAGG ACACCTACGG CACTGTGAAA 
CAAGAGCGGG ATCAGGACGA TGTCAGCATG TGCACCTCCG AGGAGGATCC GCCTAGTGGA 
TGTGCGGCCA CGACAAGTGG GCGCCAGCCC CAGATCGTGC ACCAAAATGC GCAGCTGCTG 
AACGACAGCC TGGAGCATAT GCTGGACCGC GAGCAGGAGC AGTTGGAGGC ACGTGCCAGG 
CCCTATCTGA GCAAGATACC CGAGGGGGCC GCCAACAGAT CCATGGTGGA CTTGGCCAGC 
GCCTCAAGAA ATCGCACCAA TTGGTTTGTG GCAGACGGTT CTACCGTCAC ACCCACACCC 
ACAGCCACAG TCACCTCCAC GTCCAGCAGC AGCACAAAGC CAGCACTACC AATCGAAGGC 
GACAGCCCAC CCACCTACCT GGAAGCCATT GGAGGAACAG GAACCAGCGG ACAGACCGCC 
CCTGTCAATA GCGAGTCTCG TTCTACGCTT AGCTCTCGTC TGCGGAAGAC GATAACCACC 
TTCTCGAGCG TCAAGCTGCG CATGGATGTG ATGAAGAGGC GCTCTAGCTT TAAGGCGGGG 
GCCCAGAGGC AGTCGGAGGT GAAGGTGGCC CTGCAGATGG TCCCCCGGCC CAGTCTGTCG 
CCGCTTCTGG TGCGCTACGA AGGTCCCCTT GTGCGGTTCC CCTCGGGCGT GGTCGAAGAT 
ATACTCAAGG AGATGCAGAA CCGAAAGGCC ATACTCAGGG ACCGCCAGTT CCAGACATTT 
CTGGACCAGG AGATGAAGAC TCCAAGGGAG TCGATACCGT TGGACACCAT AACCACGCTG 
CAGTGCGTCA GCAACAATCG CGTCACGGAC ACGGCGACCC ACTTTTACTG CTTCGAGATC 
ACGACATCGC AGCCGAAAAA CGGAGGCAAC GGCAACGGAG CAAGCGACAG CATGTCCTCG 
AATCCCAATC TGGTGATGAC CAGCAGCTCG TCAGGGAACG TCAAGCAGCA GCGGGTTTCC 
CATCTGTATG GCGTGGGCAA GGAGTCCGAG AGGGGCGTCT GGATGCAGAA GATCCTTGAG 
AGTCTCACCA ACAGTATGCC TGTCAAGTAC ACATGCCACT ATTACCGCGC GGGTTGGTGC 
TACCTAAAGA ACTCCATCAC GTCAGAGTGG AGTGGCACCT GGTTGGTGCT TCGCAAGAGC 
CAGCGACGTC TCATCTTCGT GTGCGAGGCC ACTGGGAATG TGGAAAAAAT GGACTTGCGC 
AAGGCGCGAT GCATAGTGCT CAAGGAGAGT GACGAGTCCA TCGACAACCT GCACGTGGGG 
ATCGGGCCAA TGCTGATGAT TGACTGTCCG CCGTATGCTG TCTACATGAT CATGAGCTCG 
GCACGAGAGA CAAAGATCTG GCGTCACATC ATTCGGGAAG TGGCACACAA CAACGGCTTT 
TCGCTGGGGG ATCAGCAGTT GACGCGGTAC GATGTACCCG TGATAGTGGA TAAGTGCATT 
AACTTTGTGT ACATCCACGG GTCCATGTCG GAGGGGATCT ATCGCAAATC AGGCTCGGAG 
AACTCCATGC ACAAGCTGAT GAGCGCCTTC CGAGCGGATG CCTTCAATGT GGAGATCACC 
CGAAACGAGT ACAATGAGCA CGATGTAGCC AATGTCCTCA AGCGGTTCAT GAGGGACCTG 
CCCGAGCGCC TCCTGGGCAA GCTAACCGAC AGCTTCGTCT TCGTCACCGA GCTGAATTTG 
GCCGCCGAGA AGATTCCCAT CTATCGTGAG CTCCTCGCTC GCCTCTCGCC CATCGAGCAT 
GAGACCTTGC GCCGGATCGT GGGCCACCTT GTGTTCATTA GCTCGCAGCA GACCAAGAAC 
AAGATGAGCG TTCAGAATCT AACCATGATC TGGGGCCCAA CGCTGCTGGC CAAGAAGAGC 
GATGAGCTAA TCTACTCTCA GAAAGAGGCC GATGTTCTCA GCGATCTCAT TCTTCTGTAC 
AAGAACCTAT TCCCCGTCAG TGCCGATGAA ATTAAACGGG AGCAGGCCAT GCTGGCATGC 
CTGCAAAAGT ATTACGCTGC CGCCGAGACG CTCAAAGATG CGGTCAAGCA GTCGGGGGAC 
ATAAAGATCT GGATCAGCCT GAATCCGAAT CCAGACAACA TCACAGAGGA AAAGATGCAG 
GTGAATGCCA CGATTTCACC CACCAAAACC GCCTACGAGC TGTGTCGGGA ATATTCCGTC 
AAAATGCAAC TACCAACCCA TCAGCTGACG CTCTACGAGG TGATACTGAA CGGCAGCCTG 
GAGCGGCCCC TGCACCATGA CACAAAAGTG TTCGACGTGA TCCTTAACTG GTCGTACTGG 
CCGGAGGAAG ATCGCAAGCA CAACTATCTG ATGGTGCGGC CCATGGAGAT GCTGCGCGAA 
ATACAGCGGG CGATCAAGAA TCTGGCCACG GTGACGCCCG GCAAGGAGCT GCGCTTCGCC 
GACAATCGCA CCAAGACGTT CAAATCTCTA CAGTGCGAGC TGCGCGACGG CAAGATTGTT 
ATTTCCAAGA AGGACAAAAA CGACAAGACA ACTATTATCA GGGAGATATT TCTGCAGAGC 
TGTACGGCGT ATGTGGGCTG TGAGCGCAAA CGAGACTTTC CATGGAGCTG GGCCATCACT 
TTTGTCGAGC GCACCCAGGC ACAGATAATG AGATCGCGAG ATGCTCCCTA CATTGGCCAT 
GTTCTGGCCG GCAGCGAGTG GGTGGATCGT ACTATTTGGT ATTCGAGCAT CTGGTATTGC 
CTCTACGGTG ACAATATTTT ACCACCAGCT GAGATCATCC TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001354329.3
CDS	612..5399
Translation	MEQAPRPVPAPRRLCTELRPANYENVEIKPSNNNNNNNTIISFKKLNINAENLHGNCAEKKLPASGQHKLQVLQENSAEQQQPIYGPDANENVPTEPIYAAPRPAPRQRLTTESLPQEPQVELDVDAVPLRAVRTAPQVPPKPPSLEQRLSICSEMEQQGDATASSLYGSNASLDCCSQGSQSGKFKSQSPGYVDDVDQRGQPFPEDSSDPETPPPTPTPPPPSTSSSTSLDQSSSCDQEQDQDREDDKNLLRSLGTTSKLLGEAIGERITFKAKGAKKRLDRNLRSSTEAISNLSADAGSRLKNASRRFGSNFTLGGRGGHGDREAEGGESSVGEFDLERRQTMPNTDVFNAIQFSSPLNRNVVGGGFTDLRDEAKVTAKVIVKDQEVPVGGDGAGDDDGCYEVPKVQGKPPSYDEALRARPSPPPPLAKNLSQEHVQLRTQQRPPIRGDDATTSSPPSSLPMPAFPPPRLSQQPERFRLDALQPPVRQKRRKNYEHIELRRPPVDSAELKELNQAAAVAEREESLLICAQIAAEEARAPKPERSDSWQFYGEDVASQQGEAVQEEDSSREPVYANQEATYGKLFEMATAGSSRGCNLLTPNQQQQQNQQQQELACISEDGELDSNDGAVGGWCAPQELIEEFDPLMSSKCSTLERSNKSNELLLLEHLLEEDTYGTVKQERDQDDVSMCTSEEDPPSGCAATTSGRQPQIVHQNAQLLNDSLEHMLDREQEQLEARARPYLSKIPEGAANRSMVDLASASRNRTNWFVADGSTVTPTPTATVTSTSSSSTKPALPIEGDSPPTYLEAIGGTGTSGQTAPVNSESRSTLSSRLRKTITTFSSVKLRMDVMKRRSSFKAGAQRQSEVKVALQMVPRPSLSPLLVRYEGPLVRFPSGVVEDILKEMQNRKAILRDRQFQTFLDQEMKTPRESIPLDTITTLQCVSNNRVTDTATHFYCFEITTSQPKNGGNGNGASDSMSSNPNLVMTSSSSGNVKQQRVSHLYGVGKESERGVWMQKILESLTNSMPVKYTCHYYRAGWCYLKNSITSEWSGTWLVLRKSQRRLIFVCEATGNVEKMDLRKARCIVLKESDESIDNLHVGIGPMLMIDCPPYAVYMIMSSARETKIWRHIIREVAHNNGFSLGDQQLTRYDVPVIVDKCINFVYIHGSMSEGIYRKSGSENSMHKLMSAFRADAFNVEITRNEYNEHDVANVLKRFMRDLPERLLGKLTDSFVFVTELNLAAEKIPIYRELLARLSPIEHETLRRIVGHLVFISSQQTKNKMSVQNLTMIWGPTLLAKKSDELIYSQKEADVLSDLILLYKNLFPVSADEIKREQAMLACLQKYYAAAETLKDAVKQSGDIKIWISLNPNPDNITEEKMQVNATISPTKTAYELCREYSVKMQLPTHQLTLYEVILNGSLERPLHHDTKVFDVILNWSYWPEEDRKHNYLMVRPMEMLREIQRAIKNLATVTPGKELRFADNRTKTFKSLQCELRDGKIVISKKDKNDKTTIIREIFLQSCTAYVGCERKRDFPWSWAITFVERTQAQIMRSRDAPYIGHVLAGSEWVDRTIWYSSIWYCLYGDNILPPAEIILK

Target ORF information:

RefSeq Version	XM_001354293.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA18539), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001354293.3

ORF Insert Sequence:

ATGGAACAGG CTCCGCGACC CGTGCCGGCT CCGCGGCGGC TCTGCACGGA GCTACGTCCC 
GCCAACTATG AGAACGTCGA AATCAAGCCT AGCAACAACA ATAACAACAA CAATACTATC 
ATCAGTTTCA AGAAGCTTAA CATCAACGCG GAGAACTTGC ACGGCAATTG TGCGGAGAAG 
AAGCTGCCGG CAAGTGGGCA GCACAAGCTG CAAGTGCTGC AGGAGAACAG CGCCGAGCAG 
CAGCAGCCCA TCTATGGTCC CGACGCCAAC GAGAATGTCC CGACAGAACC GATCTATGCC 
GCGCCCCGAC CAGCGCCGAG ACAGCGACTC ACCACGGAGT CGCTCCCGCA GGAGCCGCAA 
GTCGAACTTG ATGTGGACGC GGTTCCTCTG AGAGCTGTGC GCACAGCTCC CCAGGTGCCT 
CCGAAGCCGC CCAGCCTGGA ACAGCGCTTG TCGATATGCA GCGAGATGGA GCAGCAGGGG 
GATGCTACGG CCTCCTCTCT CTACGGCTCG AACGCCTCGC TGGACTGCTG CTCGCAGGGC 
TCGCAGAGCG GCAAGTTCAA GTCGCAGTCA CCCGGGTACG TAGACGACGT TGATCAGCGG 
GGTCAGCCAT TTCCGGAAGA TTCCTCTGAC CCGGAAACAC CTCCGCCAAC GCCAACACCA 
CCACCACCTT CGACATCTTC ATCGACGTCG CTCGATCAGT CCTCCAGCTG TGATCAAGAA 
CAAGACCAAG ATCGAGAAGA TGACAAGAAT CTTCTGCGAT CCTTGGGCAC CACCTCGAAA 
CTGCTGGGCG AGGCCATTGG CGAACGGATC ACGTTCAAGG CCAAGGGTGC CAAGAAGCGT 
CTGGACAGGA ACCTAAGGAG CTCCACGGAG GCGATCAGCA ATCTCAGCGC TGACGCTGGC 
AGCCGTCTGA AGAATGCCAG CAGGCGATTC GGGTCGAACT TCACCTTGGG GGGCAGAGGA 
GGCCATGGGG ACAGAGAAGC GGAGGGTGGA GAAAGCTCCG TGGGAGAGTT TGACCTTGAG 
CGACGGCAGA CCATGCCCAA CACGGATGTC TTCAATGCGA TACAGTTCTC CAGTCCCCTG 
AACCGGAATG TCGTCGGCGG AGGATTCACC GATCTCCGGG ATGAGGCAAA GGTCACTGCC 
AAGGTCATAG TTAAGGATCA AGAGGTGCCA GTTGGGGGGG ACGGTGCCGG GGATGATGAC 
GGCTGTTATG AGGTGCCCAA GGTGCAGGGC AAGCCCCCCA GCTACGATGA GGCACTGAGG 
GCACGTCCCT CCCCACCTCC CCCGCTGGCC AAGAATCTCT CTCAGGAGCA CGTCCAGCTT 
CGGACGCAGC AGCGTCCCCC GATCCGCGGA GATGACGCAA CGACGTCGTC TCCGCCATCG 
TCCCTGCCAA TGCCCGCGTT CCCTCCGCCG CGCCTCTCCC AACAGCCAGA GCGGTTCCGG 
CTGGACGCAC TGCAGCCGCC CGTGCGGCAG AAGCGTCGCA AAAACTACGA GCACATCGAG 
TTGCGACGTC CCCCCGTGGA CTCCGCCGAG CTGAAGGAAC TGAATCAGGC AGCGGCGGTA 
GCGGAGCGAG AGGAATCGCT GCTAATCTGC GCCCAGATTG CCGCCGAAGA GGCGAGGGCC 
CCAAAGCCAG AGCGTAGCGA TTCCTGGCAG TTCTACGGCG AGGATGTCGC TTCGCAGCAG 
GGAGAAGCGG TGCAGGAAGA AGACTCCTCC CGGGAACCTG TCTATGCCAA CCAGGAAGCC 
ACCTACGGAA AGCTCTTCGA GATGGCCACC GCGGGGAGCA GTCGCGGCTG CAACTTGTTG 
ACCCCCAACC AGCAGCAGCA GCAGAACCAG CAGCAGCAAG AGCTGGCCTG CATCAGCGAA 
GATGGGGAGC TGGACAGCAA CGACGGAGCC GTCGGTGGAT GGTGCGCGCC CCAGGAGCTG 
ATCGAGGAGT TCGATCCATT AATGAGCAGC AAGTGCTCGA CGCTGGAGCG TTCCAACAAG 
AGCAACGAGC TGCTACTGCT CGAGCACCTA CTGGAGGAGG ACACCTACGG CACTGTGAAA 
CAAGAGCGGG ATCAGGACGA TGTCAGCATG TGCACCTCCG AGGAGGATCC GCCTAGTGGA 
TGTGCGGCCA CGACAAGTGG GCGCCAGCCC CAGATCGTGC ACCAAAATGC GCAGCTGCTG 
AACGACAGCC TGGAGCATAT GCTGGACCGC GAGCAGGAGC AGTTGGAGGC ACGTGCCAGG 
CCCTATCTGA GCAAGATACC CGAGGGGGCC GCCAACAGAT CCATGGTGGA CTTGGCCAGC 
GCCTCAAGAA ATCGCACCAA TTGGTTTGTG GCAGACGGTT CTACCGTCAC ACCCACACCC 
ACAGCCACAG TCACCTCCAC GTCCAGCAGC AGCACAAAGC CAGCACTACC AATCGAAGGC 
GACAGCCCAC CCACCTACCT GGAAGCCATT GGAGGAACAG GAACCAGCGG ACAGACCGCC 
CCTGTCAATA GCGAGTCTCG TTCTACGCTT AGCTCTCGTC TGCGGAAGAC GATAACCACC 
TTCTCGAGCG TCAAGCTGCG CATGGATGTG ATGAAGAGGC GCTCTAGCTT TAAGGCGGGG 
GCCCAGAGGC AGTCGGAGGT GAAGGTGGCC CTGCAGATGG TCCCCCGGCC CAGTCTGTCG 
CCGCTTCTGG TGCGCTACGA AGGTCCCCTT GTGCGGTTCC CCTCGGGCGT GGTCGAAGAT 
ATACTCAAGG AGATGCAGAA CCGAAAGGCC ATACTCAGGG ACCGCCAGTT CCAGACATTT 
CTGGACCAGG AGATGAAGAC TCCAAGGGAG TCGATACCGT TGGACACCAT AACCACGCTG 
CAGTGCGTCA GCAACAATCG CGTCACGGAC ACGGCGACCC ACTTTTACTG CTTCGAGATC 
ACGACATCGC AGCCGAAAAA CGGAGGCAAC GGCAACGGAG CAAGCGACAG CATGTCCTCG 
AATCCCAATC TGGTGATGAC CAGCAGCTCG TCAGGGAACG TCAAGCAGCA GCGGGTTTCC 
CATCTGTATG GCGTGGGCAA GGAGTCCGAG AGGGGCGTCT GGATGCAGAA GATCCTTGAG 
AGTCTCACCA ACAGTATGCC TGTCAAGTAC ACATGCCACT ATTACCGCGC GGGTTGGTGC 
TACCTAAAGA ACTCCATCAC GTCAGAGTGG AGTGGCACCT GGTTGGTGCT TCGCAAGAGC 
CAGCGACGTC TCATCTTCGT GTGCGAGGCC ACTGGGAATG TGGAAAAAAT GGACTTGCGC 
AAGGCGCGAT GCATAGTGCT CAAGGAGAGT GACGAGTCCA TCGACAACCT GCACGTGGGG 
ATCGGGCCAA TGCTGATGAT TGACTGTCCG CCGTATGCTG TCTACATGAT CATGAGCTCG 
GCACGAGAGA CAAAGATCTG GCGTCACATC ATTCGGGAAG TGGCACACAA CAACGGCTTT 
TCGCTGGGGG ATCAGCAGTT GACGCGGTAC GATGTACCCG TGATAGTGGA TAAGTGCATT 
AACTTTGTGT ACATCCACGG GTCCATGTCG GAGGGGATCT ATCGCAAATC AGGCTCGGAG 
AACTCCATGC ACAAGCTGAT GAGCGCCTTC CGAGCGGATG CCTTCAATGT GGAGATCACC 
CGAAACGAGT ACAATGAGCA CGATGTAGCC AATGTCCTCA AGCGGTTCAT GAGGGACCTG 
CCCGAGCGCC TCCTGGGCAA GCTAACCGAC AGCTTCGTCT TCGTCACCGA GCTGAATTTG 
GCCGCCGAGA AGATTCCCAT CTATCGTGAG CTCCTCGCTC GCCTCTCGCC CATCGAGCAT 
GAGACCTTGC GCCGGATCGT GGGCCACCTT GTGTTCATTA GCTCGCAGCA GACCAAGAAC 
AAGATGAGCG TTCAGAATCT AACCATGATC TGGGGCCCAA CGCTGCTGGC CAAGAAGAGC 
GATGAGCTAA TCTACTCTCA GAAAGAGGCC GATGTTCTCA GCGATCTCAT TCTTCTGTAC 
AAGAACCTAT TCCCCGTCAG TGCCGATGAA ATTAAACGGG AGCAGGCCAT GCTGGCATGC 
CTGCAAAAGT ATTACGCTGC CGCCGAGACG CTCAAAGATG CGGTCAAGCA GTCGGGGGAC 
ATAAAGATCT GGATCAGCCT GAATCCGAAT CCAGACAACA TCACAGAGGA AAAGATGCAG 
GTGAATGCCA CGATTTCACC CACCAAAACC GCCTACGAGC TGTGTCGGGA ATATTCCGTC 
AAAATGCAAC TACCAACCCA TCAGCTGACG CTCTACGAGG TGATACTGAA CGGCAGCCTG 
GAGCGGCCCC TGCACCATGA CACAAAAGTG TTCGACGTGA TCCTTAACTG GTCGTACTGG 
CCGGAGGAAG ATCGCAAGCA CAACTATCTG ATGGTGCGGC CCATGGAGAT GCTGCGCGAA 
ATACAGCGGG CGATCAAGAA TCTGGCCACG GTGACGCCCG GCAAGGAGCT GCGCTTCGCC 
GACAATCGCA CCAAGACGTT CAAATCTCTA CAGTGCGAGC TGCGCGACGG CAAGATTGTT 
ATTTCCAAGA AGGACAAAAA CGACAAGACA ACTATTATCA GGGAGATATT TCTGCAGAGC 
TGTACGGCGT ATGTGGGCTG TGAGCGCAAA CGAGACTTTC CATGGAGCTG GGCCATCACT 
TTTGTCGAGC GCACCCAGGC ACAGATAATG AGATCGCGAG ATGCTCCCTA CATTGGCCAT 
GTTCTGGCCG GCAGCGAGTG GGTGGATCGT ACTATTTGGT ATTCGAGCAT CTGGTATTGC 
CTCTACGGTG ACAATATTTT ACCACCAGCT GAGATCATCC TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA18539 ( XM_015186908.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015042394.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA18539), mRNA.

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CloneID	ODe25423
Clone ID Related Accession (Same CDS sequence)	XM_015186908.1
Accession Version	XM_015186908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589958). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACAGG CTCCGCGACC CGTGCCGGCT CCGCGGCGGC TCTGCACGGA GCTACGTCCC 
GCCAACTATG AGAACGTCGA AATCAAGCCT AGCAACAACA ATAACAACAA CAATACTATC 
ATCAGTTTCA AGAAGCTTAA CATCAACGCG GAGAACTTGC ACGGCAATTG TGCGGAGAAG 
AAGCTGCCGG CAAGTGGGCA GCACAAGCTG CAAGTGCTGC AGGAGAACAG CGCCGAGCAG 
CAGCAGCCCA TCTATGGTCC CGACGCCAAC GAGAATGTCC CGACAGAACC GATCTATGCC 
GCGCCCCGAC CAGCGCCGAG ACAGCGACTC ACCACGGAGT CGCTCCCGCA GGAGCCGCAA 
GTCGAACTTG ATGTGGACGC GGTTCCTCTG AGAGCTGTGC GCACAGCTCC CCAGGTGCCT 
CCGAAGCCGC CCAGCCTGGA ACAGCGCTTG TCGATATGCA GCGAGATGGA GCAGCAGGGG 
GATGCTACGG CCTCCTCTCT CTACGGCTCG AACGCCTCGC TGGACTGCTG CTCGCAGGGC 
TCGCAGAGCG GCAAGTTCAA GTCGCAGTCA CCCGGGAATC TTCTGCGATC CTTGGGCACC 
ACCTCGAAAC TGCTGGGCGA GGCCATTGGC GAACGGATCA CGTTCAAGGC CAAGGGTGCC 
AAGAAGCGTC TGGACAGGAA CCTAAGGAGC TCCACGGAGG CGATCAGCAA TCTCAGCGCT 
GACGCTGGCA GCCGTCTGAA GAATGCCAGC AGGCGATTCG GGTCGAACTT CACCTTGGGG 
GGCAGAGGAG GCCATGGGGA CAGAGAAGCG GAGGGTGGAG AAAGCTCCGT GGGAGAGTTT 
GACCTTGAGC GACGGCAGAC CATGCCCAAC ACGGATGTCT TCAATGCGAT ACAGTTCTCC 
AGTCCCCTGA ACCGGAATGT CGTCGGCGGA GGATTCACCG ATCTCCGGGA TGAGGCAAAG 
GTCACTGCCA AGGTCATAGT TAAGGATCAA GAGGTGCCAG TTGGGGGGGA CGGTGCCGGG 
GATGATGACG GCTGTTATGA GGTGCCCAAG GTGCAGGGCA AGCCCCCCAG CTACGATGAG 
GCACTGAGGG CACGTCCCTC CCCACCTCCC CCGCTGGCCA AGAATCTCTC TCAGGAGCAC 
GTCCAGCTTC GGACGCAGCA GCGTCCCCCG ATCCGCGGAG ATGACGCAAC GACGTCGTCT 
CCGCCATCGT CCCTGCCAAT GCCCGCGTTC CCTCCGCCGC GCCTCTCCCA ACAGCCAGAG 
CGGTTCCGGC TGGACGCACT GCAGCCGCCC GTGCGGCAGA AGCGTCGCAA AAACTACGAG 
CACATCGAGT TGCGACGTCC CCCCGTGGAC TCCGCCGAGC TGAAGGAACT GAATCAGGCA 
GCGGCGGTAG CGGAGCGAGA GGAATCGCTG CTAATCTGCG CCCAGATTGC CGCCGAAGAG 
GCGAGGGCCC CAAAGCCAGA GCGTAGCGAT TCCTGGCAGT TCTACGGCGA GGATGTCGCT 
TCGCAGCAGG GAGAAGCGGT GCAGGAAGAA GACTCCTCCC GGGAACCTGT CTATGCCAAC 
CAGGAAGCCA CCTACGGAAA GCTCTTCGAG ATGGCCACCG CGGGGAGCAG TCGCGGCTGC 
AACTTGTTGA CCCCCAACCA GCAGCAGCAG CAGAACCAGC AGCAGCAAGA GCTGGCCTGC 
ATCAGCGAAG ATGGGGAGCT GGACAGCAAC GACGGAGCCG TCGGTGGATG GTGCGCGCCC 
CAGGAGCTGA TCGAGGAGTT CGATCCATTA ATGAGCAGCA AGTGCTCGAC GCTGGAGCGT 
TCCAACAAGA GCAACGAGCT GCTACTGCTC GAGCACCTAC TGGAGGAGGA CACCTACGGC 
ACTGTGAAAC AAGAGCGGGA TCAGGACGAT GTCAGCATGT GCACCTCCGA GGAGGATCCG 
CCTAGTGGAT GTGCGGCCAC GACAAGTGGG CGCCAGCCCC AGATCGTGCA CCAAAATGCG 
CAGCTGCTGA ACGACAGCCT GGAGCATATG CTGGACCGCG AGCAGGAGCA GTTGGAGGCA 
CGTGCCAGGC CCTATCTGAG CAAGATACCC GAGGGGGCCG CCAACAGATC CATGGTGGAC 
TTGGCCAGCG CCTCAAGAAA TCGCACCAAT TGGTTTGTGG CAGACGGTTC TACCGTCACA 
CCCACACCCA CAGCCACAGT CACCTCCACG TCCAGCAGCA GCACAAAGCC AGCACTACCA 
ATCGAAGGCG ACAGCCCACC CACCTACCTG GAAGCCATTG GAGGAACAGG AACCAGCGGA 
CAGACCGCCC CTGTCAATAG CGAGTCTCGT TCTACGCTTA GCTCTCGTCT GCGGAAGACG 
ATAACCACCT TCTCGAGCGT CAAGCTGCGC ATGGATGTGA TGAAGAGGCG CTCTAGCTTT 
AAGGCGGGGG CCCAGAGGCA GTCGGAGGTG AAGGTGGCCC TGCAGATGGT CCCCCGGCCC 
AGTCTGTCGC CGCTTCTGGT GCGCTACGAA GGTCCCCTTG TGCGGTTCCC CTCGGGCGTG 
GTCGAAGATA TACTCAAGGA GATGCAGAAC CGAAAGGCCA TACTCAGGGA CCGCCAGTTC 
CAGACATTTC TGGACCAGGA GATGAAGACT CCAAGGGAGT CGATACCGTT GGACACCATA 
ACCACGCTGC AGTGCGTCAG CAACAATCGC GTCACGGACA CGGCGACCCA CTTTTACTGC 
TTCGAGATCA CGACATCGCA GCCGAAAAAC GGAGGCAACG GCAACGGAGC AAGCGACAGC 
ATGTCCTCGA ATCCCAATCT GGTGATGACC AGCAGCTCGT CAGGGAACGT CAAGCAGCAG 
CGGGTTTCCC ATCTGTATGG CGTGGGCAAG GAGTCCGAGA GGGGCGTCTG GATGCAGAAG 
ATCCTTGAGA GTCTCACCAA CAGTATGCCT GTCAAGTACA CATGCCACTA TTACCGCGCG 
GGTTGGTGCT ACCTAAAGAA CTCCATCACG TCAGAGTGGA GTGGCACCTG GTTGGTGCTT 
CGCAAGAGCC AGCGACGTCT CATCTTCGTG TGCGAGGCCA CTGGGAATGT GGAAAAAATG 
GACTTGCGCA AGGCGCGATG CATAGTGCTC AAGGAGAGTG ACGAGTCCAT CGACAACCTG 
CACGTGGGGA TCGGGCCAAT GCTGATGATT GACTGTCCGC CGTATGCTGT CTACATGATC 
ATGAGCTCGG CACGAGAGAC AAAGATCTGG CGTCACATCA TTCGGGAAGT GGCACACAAC 
AACGGCTTTT CGCTGGGGGA TCAGCAGTTG ACGCGGTACG ATGTACCCGT GATAGTGGAT 
AAGTGCATTA ACTTTGTGTA CATCCACGGG TCCATGTCGG AGGGGATCTA TCGCAAATCA 
GGCTCGGAGA ACTCCATGCA CAAGCTGATG AGCGCCTTCC GAGCGGATGC CTTCAATGTG 
GAGATCACCC GAAACGAGTA CAATGAGCAC GATGTAGCCA ATGTCCTCAA GCGGTTCATG 
AGGGACCTGC CCGAGCGCCT CCTGGGCAAG CTAACCGACA GCTTCGTCTT CGTCACCGAG 
CTGAATTTGG CCGCCGAGAA GATTCCCATC TATCGTGAGC TCCTCGCTCG CCTCTCGCCC 
ATCGAGCATG AGACCTTGCG CCGGATCGTG GGCCACCTTG TGTTCATTAG CTCGCAGCAG 
ACCAAGAACA AGATGAGCGT TCAGAATCTA ACCATGATCT GGGGCCCAAC GCTGCTGGCC 
AAGAAGAGCG ATGAGCTAAT CTACTCTCAG AAAGAGGCCG ATGTTCTCAG CGATCTCATT 
CTTCTGTACA AGAACCTATT CCCCGTCAGT GCCGATGAAA TTAAACGGGA GCAGGCCATG 
CTGGCATGCC TGCAAAAGTA TTACGCTGCC GCCGAGACGC TCAAAGATGC GGTCAAGCAG 
TCGGGGGACA TAAAGATCTG GATCAGCCTG AATCCGAATC CAGACAACAT CACAGAGGAA 
AAGATGCAGG TGAATGCCAC GATTTCACCC ACCAAAACCG CCTACGAGCT GTGTCGGGAA 
TATTCCGTCA AAATGCAACT ACCAACCCAT CAGCTGACGC TCTACGAGGT GATACTGAAC 
GGCAGCCTGG AGCGGCCCCT GCACCATGAC ACAAAAGTGT TCGACGTGAT CCTTAACTGG 
TCGTACTGGC CGGAGGAAGA TCGCAAGCAC AACTATCTGA TGGTGCGGCC CATGGAGATG 
CTGCGCGAAA TACAGCGGGC GATCAAGAAT CTGGCCACGG TGACGCCCGG CAAGGAGCTG 
CGCTTCGCCG ACAATCGCAC CAAGACGTTC AAATCTCTAC AGTGCGAGCT GCGCGACGGC 
AAGATTGTTA TTTCCAAGAA GGACAAAAAC GACAAGACAA CTATTATCAG GGAGATATTT 
CTGCAGAGCT GTACGGCGTA TGTGGGCTGT GAGCGCAAAC GAGACTTTCC ATGGAGCTGG 
GCCATCACTT TTGTCGAGCG CACCCAGGCA CAGATAATGA GATCGCGAGA TGCTCCCTAC 
ATTGGCCATG TTCTGGCCGG CAGCGAGTGG GTGGATCGTA CTATTTGGTA TTCGAGCATC 
TGGTATTGCC TCTACGGTGA CAATATTTTA CCACCAGCTG AGATCATCCT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015042394.1
CDS	610..5226
Translation	MEQAPRPVPAPRRLCTELRPANYENVEIKPSNNNNNNNTIISFKKLNINAENLHGNCAEKKLPASGQHKLQVLQENSAEQQQPIYGPDANENVPTEPIYAAPRPAPRQRLTTESLPQEPQVELDVDAVPLRAVRTAPQVPPKPPSLEQRLSICSEMEQQGDATASSLYGSNASLDCCSQGSQSGKFKSQSPGNLLRSLGTTSKLLGEAIGERITFKAKGAKKRLDRNLRSSTEAISNLSADAGSRLKNASRRFGSNFTLGGRGGHGDREAEGGESSVGEFDLERRQTMPNTDVFNAIQFSSPLNRNVVGGGFTDLRDEAKVTAKVIVKDQEVPVGGDGAGDDDGCYEVPKVQGKPPSYDEALRARPSPPPPLAKNLSQEHVQLRTQQRPPIRGDDATTSSPPSSLPMPAFPPPRLSQQPERFRLDALQPPVRQKRRKNYEHIELRRPPVDSAELKELNQAAAVAEREESLLICAQIAAEEARAPKPERSDSWQFYGEDVASQQGEAVQEEDSSREPVYANQEATYGKLFEMATAGSSRGCNLLTPNQQQQQNQQQQELACISEDGELDSNDGAVGGWCAPQELIEEFDPLMSSKCSTLERSNKSNELLLLEHLLEEDTYGTVKQERDQDDVSMCTSEEDPPSGCAATTSGRQPQIVHQNAQLLNDSLEHMLDREQEQLEARARPYLSKIPEGAANRSMVDLASASRNRTNWFVADGSTVTPTPTATVTSTSSSSTKPALPIEGDSPPTYLEAIGGTGTSGQTAPVNSESRSTLSSRLRKTITTFSSVKLRMDVMKRRSSFKAGAQRQSEVKVALQMVPRPSLSPLLVRYEGPLVRFPSGVVEDILKEMQNRKAILRDRQFQTFLDQEMKTPRESIPLDTITTLQCVSNNRVTDTATHFYCFEITTSQPKNGGNGNGASDSMSSNPNLVMTSSSSGNVKQQRVSHLYGVGKESERGVWMQKILESLTNSMPVKYTCHYYRAGWCYLKNSITSEWSGTWLVLRKSQRRLIFVCEATGNVEKMDLRKARCIVLKESDESIDNLHVGIGPMLMIDCPPYAVYMIMSSARETKIWRHIIREVAHNNGFSLGDQQLTRYDVPVIVDKCINFVYIHGSMSEGIYRKSGSENSMHKLMSAFRADAFNVEITRNEYNEHDVANVLKRFMRDLPERLLGKLTDSFVFVTELNLAAEKIPIYRELLARLSPIEHETLRRIVGHLVFISSQQTKNKMSVQNLTMIWGPTLLAKKSDELIYSQKEADVLSDLILLYKNLFPVSADEIKREQAMLACLQKYYAAAETLKDAVKQSGDIKIWISLNPNPDNITEEKMQVNATISPTKTAYELCREYSVKMQLPTHQLTLYEVILNGSLERPLHHDTKVFDVILNWSYWPEEDRKHNYLMVRPMEMLREIQRAIKNLATVTPGKELRFADNRTKTFKSLQCELRDGKIVISKKDKNDKTTIIREIFLQSCTAYVGCERKRDFPWSWAITFVERTQAQIMRSRDAPYIGHVLAGSEWVDRTIWYSSIWYCLYGDNILPPAEIILK

Target ORF information:

RefSeq Version	XM_015186908.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA18539), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015186908.1

ORF Insert Sequence:

ATGGAACAGG CTCCGCGACC CGTGCCGGCT CCGCGGCGGC TCTGCACGGA GCTACGTCCC 
GCCAACTATG AGAACGTCGA AATCAAGCCT AGCAACAACA ATAACAACAA CAATACTATC 
ATCAGTTTCA AGAAGCTTAA CATCAACGCG GAGAACTTGC ACGGCAATTG TGCGGAGAAG 
AAGCTGCCGG CAAGTGGGCA GCACAAGCTG CAAGTGCTGC AGGAGAACAG CGCCGAGCAG 
CAGCAGCCCA TCTATGGTCC CGACGCCAAC GAGAATGTCC CGACAGAACC GATCTATGCC 
GCGCCCCGAC CAGCGCCGAG ACAGCGACTC ACCACGGAGT CGCTCCCGCA GGAGCCGCAA 
GTCGAACTTG ATGTGGACGC GGTTCCTCTG AGAGCTGTGC GCACAGCTCC CCAGGTGCCT 
CCGAAGCCGC CCAGCCTGGA ACAGCGCTTG TCGATATGCA GCGAGATGGA GCAGCAGGGG 
GATGCTACGG CCTCCTCTCT CTACGGCTCG AACGCCTCGC TGGACTGCTG CTCGCAGGGC 
TCGCAGAGCG GCAAGTTCAA GTCGCAGTCA CCCGGGAATC TTCTGCGATC CTTGGGCACC 
ACCTCGAAAC TGCTGGGCGA GGCCATTGGC GAACGGATCA CGTTCAAGGC CAAGGGTGCC 
AAGAAGCGTC TGGACAGGAA CCTAAGGAGC TCCACGGAGG CGATCAGCAA TCTCAGCGCT 
GACGCTGGCA GCCGTCTGAA GAATGCCAGC AGGCGATTCG GGTCGAACTT CACCTTGGGG 
GGCAGAGGAG GCCATGGGGA CAGAGAAGCG GAGGGTGGAG AAAGCTCCGT GGGAGAGTTT 
GACCTTGAGC GACGGCAGAC CATGCCCAAC ACGGATGTCT TCAATGCGAT ACAGTTCTCC 
AGTCCCCTGA ACCGGAATGT CGTCGGCGGA GGATTCACCG ATCTCCGGGA TGAGGCAAAG 
GTCACTGCCA AGGTCATAGT TAAGGATCAA GAGGTGCCAG TTGGGGGGGA CGGTGCCGGG 
GATGATGACG GCTGTTATGA GGTGCCCAAG GTGCAGGGCA AGCCCCCCAG CTACGATGAG 
GCACTGAGGG CACGTCCCTC CCCACCTCCC CCGCTGGCCA AGAATCTCTC TCAGGAGCAC 
GTCCAGCTTC GGACGCAGCA GCGTCCCCCG ATCCGCGGAG ATGACGCAAC GACGTCGTCT 
CCGCCATCGT CCCTGCCAAT GCCCGCGTTC CCTCCGCCGC GCCTCTCCCA ACAGCCAGAG 
CGGTTCCGGC TGGACGCACT GCAGCCGCCC GTGCGGCAGA AGCGTCGCAA AAACTACGAG 
CACATCGAGT TGCGACGTCC CCCCGTGGAC TCCGCCGAGC TGAAGGAACT GAATCAGGCA 
GCGGCGGTAG CGGAGCGAGA GGAATCGCTG CTAATCTGCG CCCAGATTGC CGCCGAAGAG 
GCGAGGGCCC CAAAGCCAGA GCGTAGCGAT TCCTGGCAGT TCTACGGCGA GGATGTCGCT 
TCGCAGCAGG GAGAAGCGGT GCAGGAAGAA GACTCCTCCC GGGAACCTGT CTATGCCAAC 
CAGGAAGCCA CCTACGGAAA GCTCTTCGAG ATGGCCACCG CGGGGAGCAG TCGCGGCTGC 
AACTTGTTGA CCCCCAACCA GCAGCAGCAG CAGAACCAGC AGCAGCAAGA GCTGGCCTGC 
ATCAGCGAAG ATGGGGAGCT GGACAGCAAC GACGGAGCCG TCGGTGGATG GTGCGCGCCC 
CAGGAGCTGA TCGAGGAGTT CGATCCATTA ATGAGCAGCA AGTGCTCGAC GCTGGAGCGT 
TCCAACAAGA GCAACGAGCT GCTACTGCTC GAGCACCTAC TGGAGGAGGA CACCTACGGC 
ACTGTGAAAC AAGAGCGGGA TCAGGACGAT GTCAGCATGT GCACCTCCGA GGAGGATCCG 
CCTAGTGGAT GTGCGGCCAC GACAAGTGGG CGCCAGCCCC AGATCGTGCA CCAAAATGCG 
CAGCTGCTGA ACGACAGCCT GGAGCATATG CTGGACCGCG AGCAGGAGCA GTTGGAGGCA 
CGTGCCAGGC CCTATCTGAG CAAGATACCC GAGGGGGCCG CCAACAGATC CATGGTGGAC 
TTGGCCAGCG CCTCAAGAAA TCGCACCAAT TGGTTTGTGG CAGACGGTTC TACCGTCACA 
CCCACACCCA CAGCCACAGT CACCTCCACG TCCAGCAGCA GCACAAAGCC AGCACTACCA 
ATCGAAGGCG ACAGCCCACC CACCTACCTG GAAGCCATTG GAGGAACAGG AACCAGCGGA 
CAGACCGCCC CTGTCAATAG CGAGTCTCGT TCTACGCTTA GCTCTCGTCT GCGGAAGACG 
ATAACCACCT TCTCGAGCGT CAAGCTGCGC ATGGATGTGA TGAAGAGGCG CTCTAGCTTT 
AAGGCGGGGG CCCAGAGGCA GTCGGAGGTG AAGGTGGCCC TGCAGATGGT CCCCCGGCCC 
AGTCTGTCGC CGCTTCTGGT GCGCTACGAA GGTCCCCTTG TGCGGTTCCC CTCGGGCGTG 
GTCGAAGATA TACTCAAGGA GATGCAGAAC CGAAAGGCCA TACTCAGGGA CCGCCAGTTC 
CAGACATTTC TGGACCAGGA GATGAAGACT CCAAGGGAGT CGATACCGTT GGACACCATA 
ACCACGCTGC AGTGCGTCAG CAACAATCGC GTCACGGACA CGGCGACCCA CTTTTACTGC 
TTCGAGATCA CGACATCGCA GCCGAAAAAC GGAGGCAACG GCAACGGAGC AAGCGACAGC 
ATGTCCTCGA ATCCCAATCT GGTGATGACC AGCAGCTCGT CAGGGAACGT CAAGCAGCAG 
CGGGTTTCCC ATCTGTATGG CGTGGGCAAG GAGTCCGAGA GGGGCGTCTG GATGCAGAAG 
ATCCTTGAGA GTCTCACCAA CAGTATGCCT GTCAAGTACA CATGCCACTA TTACCGCGCG 
GGTTGGTGCT ACCTAAAGAA CTCCATCACG TCAGAGTGGA GTGGCACCTG GTTGGTGCTT 
CGCAAGAGCC AGCGACGTCT CATCTTCGTG TGCGAGGCCA CTGGGAATGT GGAAAAAATG 
GACTTGCGCA AGGCGCGATG CATAGTGCTC AAGGAGAGTG ACGAGTCCAT CGACAACCTG 
CACGTGGGGA TCGGGCCAAT GCTGATGATT GACTGTCCGC CGTATGCTGT CTACATGATC 
ATGAGCTCGG CACGAGAGAC AAAGATCTGG CGTCACATCA TTCGGGAAGT GGCACACAAC 
AACGGCTTTT CGCTGGGGGA TCAGCAGTTG ACGCGGTACG ATGTACCCGT GATAGTGGAT 
AAGTGCATTA ACTTTGTGTA CATCCACGGG TCCATGTCGG AGGGGATCTA TCGCAAATCA 
GGCTCGGAGA ACTCCATGCA CAAGCTGATG AGCGCCTTCC GAGCGGATGC CTTCAATGTG 
GAGATCACCC GAAACGAGTA CAATGAGCAC GATGTAGCCA ATGTCCTCAA GCGGTTCATG 
AGGGACCTGC CCGAGCGCCT CCTGGGCAAG CTAACCGACA GCTTCGTCTT CGTCACCGAG 
CTGAATTTGG CCGCCGAGAA GATTCCCATC TATCGTGAGC TCCTCGCTCG CCTCTCGCCC 
ATCGAGCATG AGACCTTGCG CCGGATCGTG GGCCACCTTG TGTTCATTAG CTCGCAGCAG 
ACCAAGAACA AGATGAGCGT TCAGAATCTA ACCATGATCT GGGGCCCAAC GCTGCTGGCC 
AAGAAGAGCG ATGAGCTAAT CTACTCTCAG AAAGAGGCCG ATGTTCTCAG CGATCTCATT 
CTTCTGTACA AGAACCTATT CCCCGTCAGT GCCGATGAAA TTAAACGGGA GCAGGCCATG 
CTGGCATGCC TGCAAAAGTA TTACGCTGCC GCCGAGACGC TCAAAGATGC GGTCAAGCAG 
TCGGGGGACA TAAAGATCTG GATCAGCCTG AATCCGAATC CAGACAACAT CACAGAGGAA 
AAGATGCAGG TGAATGCCAC GATTTCACCC ACCAAAACCG CCTACGAGCT GTGTCGGGAA 
TATTCCGTCA AAATGCAACT ACCAACCCAT CAGCTGACGC TCTACGAGGT GATACTGAAC 
GGCAGCCTGG AGCGGCCCCT GCACCATGAC ACAAAAGTGT TCGACGTGAT CCTTAACTGG 
TCGTACTGGC CGGAGGAAGA TCGCAAGCAC AACTATCTGA TGGTGCGGCC CATGGAGATG 
CTGCGCGAAA TACAGCGGGC GATCAAGAAT CTGGCCACGG TGACGCCCGG CAAGGAGCTG 
CGCTTCGCCG ACAATCGCAC CAAGACGTTC AAATCTCTAC AGTGCGAGCT GCGCGACGGC 
AAGATTGTTA TTTCCAAGAA GGACAAAAAC GACAAGACAA CTATTATCAG GGAGATATTT 
CTGCAGAGCT GTACGGCGTA TGTGGGCTGT GAGCGCAAAC GAGACTTTCC ATGGAGCTGG 
GCCATCACTT TTGTCGAGCG CACCCAGGCA CAGATAATGA GATCGCGAGA TGCTCCCTAC 
ATTGGCCATG TTCTGGCCGG CAGCGAGTGG GTGGATCGTA CTATTTGGTA TTCGAGCATC 
TGGTATTGCC TCTACGGTGA CAATATTTTA CCACCAGCTG AGATCATCCT CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA18539 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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