Dpse\GA18465 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA18465
Entrez Gene ID	4813711
Full Name	GA18465 gene product from transcript GA18465-RA
Synonyms	GA18465,dpse_GLEANR_12304
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001353724.3	XP_001353760.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA18465 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA18465 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe25835	XM_001353724.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18465), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA18465 ( XM_001353724.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001353760.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18465), partial mRNA.

Price & Availability↑

CloneID	ODe25835
Clone ID Related Accession (Same CDS sequence)	XM_001353724.3
Accession Version	XM_001353724.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). On Dec 22, 2015 this sequence version replaced XM_001353724.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTTGGCC TCAGTCCTCC CCCGTCGCAC AGCGTCTATC AAACGCTTGA CAGCAGCCAA 
CAGATCAAGC TGAAAAATTG TCCCCACCAA AGGAGCTCTG ACGACTCTGG AGGAATTAAT 
AAAGTCAATC TCTTGACATG GCTCCTGCTG ATGGTAGCTC TCGTCTGGAG CTGGCAATTC 
AATGGAGGCG TGGCCATGTC ACAGGCGGAA CCGCGCCACA AGACCTGCGA CAATGAGCAG 
CGATACGAGA ACCAGGTCAT GTGCGAGGGC AAAGAGTTTG GCGCCCTGGT GCCGTATCCG 
GATAACTGTA GTCTGTTTCT CGTCTGCGAT TGTCTGTATC CTACGGTCAA GCTCTGTCCG 
GCAGAGCTCT GGTGGGATAA TGCCTCACAG ATATGCAACT ATCCGCAGTC GGTGGATTGT 
GTCTACTACA CCATTGAAAC GCCGGAACCG ACGGATGGAA CAACGAGAAT TACGCCGGTT 
ATTACCACCG AATCAACGAC TTTGAGTACA CCAAAAGTCA CCGAATACCC AACGACATTG 
AGCACGCCAA AAGTCACCGA ACACCCAACG ACATTGAGCA CGCCAAAAGT CACCGAATAC 
CCAACGACTC CAAAAGTGAC GGACGTTCCC TGTATCACGC CCACTTCCAG CTGGGACCCG 
CCACCAGCAC CGCCTGGCAT TCCCGATGAT TTCTGTAGGC ATCAATCGGA TGGCTCGGTG 
CATCCTTATC CGTACGATTG CCAGGCGTAT ATCAACTGTA CCCATGGCTG GCCCGTCCTC 
AACTATTGCA TCGAGGATAA GGTCTTCAAT CCGTGGCTGT TCATCTGCGA TACCCACGAC 
ACAACCGAAT GCGAGGAGGA GCCGTTGCCC ACGGCCACGC CCACCACTAC AGAGATGGTT 
CCCACTACCA CCACAACAGA GTTGGCTCCC ACTTCCACCA CAGAAGCGGA CGATGTAGAG 
TGCGGACCCA CACCAGAGTT CGTGGAGGAG GATTACTGCC AGCCCTTGGG CAATGGCTTC 
TACGAGTATC CCTACAATTG CAGTGCGTAC CTCTTGTGCC GCAATGGCTG CACGGACTTG 
GACTACTGCA TATCGGATAA GTTGTTCAAT CCATGGCTCC ACATTTGCGA TACTCCCGAC 
TCAGTGTACT GCGATCCACT GCCTTATCCC ACTGCTCCAC CGTCTTCCAC GACTCCTTCG 
AGCACCACAA CTTCTGTGGA TGTTTCTACG ACGGAGAGCA CCACTGCCAC CACAGAGGCA 
GCCACAAGCA CAACGCCGGG ACTGCCCCCC GATGTGAATC CCAACGAATG TCGCGGTGTT 
CCCGATGGCA CTCGATTGCC ATATGCCGGC AACTGTTCGC AGTTCTTAAT CTGCAAGGAC 
GATCAGGTGG AGATGGGTCA GTGTCCTCCA TTGACGCTCT TTAATCCGGA TTTACTCATC 
TGCGATGAGG CTGATGATGT CTTGTGCTAT GGGGATCGCA CCACCACACC GATACCCACG 
ACAACAACCA CTGAGGCGAC AACACCGACG CCTACCACAA CGACAACGAA GGCCACAACT 
CTGGGGCCCG AAGAGCTCTG TGAGGGGCAG AGTCTGGGAG CCTCCTTCGC GTATCCCCAG 
GACTGCTCTA AGTACTATTT GTGTTTGGGA GGTGGACAAT GGTTCCTGAC AGACTGCATT 
TATGGCAGCT ACTTTGACCC CAACACAATG AATTGTGGGC CCAATGTCTC ACCAACGGCC 
TGCCAAGAGG GACAAGCAAC CACAACAACG GCAGCAACCA CAACCACCCA GAAGGTAACC 
ACTCCAACAA CGACAACAGA GGAACCATCG ACCCCGGCGG CAACGACCAC AACTCAAGCG 
CCACCGGGTG GCATTTGCGG TGGCCGGGAT GAAGGTGAAA ATATCGCCTA TCCCAAGGAT 
TGCAACAAGT ACATCGTCTG CGTCTCCCCC ATACCCATTG CCTTCTACTG CCCAGCGGGA 
TCCTACTTCA GTTCGAAGCT CCAGAACTGC GCCAGCTGGG AGGAGAGTGA CTGCGATCAG 
GATGCCAGCA CTACAACGCT ATCCCCAGGG TTAACCCAAG CGCCGCTGGA GCCAACGATG 
TGCACAAACA GCAGTCGCGA TACCTTCCCG TATCCGGACA ACTGTCAGTG GTTCATCCGC 
TGTGTAGACG ACTACATCTA CATGATGGAC GTCTGCAACT GCGGGGAGTA CTACGATCCC 
ATAAGCGGCA AGTGCGGAGC AGATGTTCCA TCGGATGCCT GTCGCTGGGA CTACACTTCG 
ACCACGTCCA CCACCAGCGA ACCGACCACC AGCACAGCCG TGACCAGACC CCCACCGCAG 
AAGGGTCCCT GCGACGATGT GGCGGATGGT GCTTTGGTGC CATATCCTAA CGATTGCACC 
AAGTACATCA AATGCGATCG CCCAATAGCC ACGGCCTTCG ACTGCGAAGA GGGGGATGAG 
TTCAGTGTCG CGCTGGGGGA GTGTGTGGAC GCTTCTCTGG CCGGTTGCTC GGTTACAACC 
ACGACCAACC CTTCGGGATC GACGACGCCA TCGATACCAT CGACAAAGAC AACAGAAGGG 
GACTCTACAA CAGATTCTTC CACAGCATCA TCCACGGAAT CATCTACTGA ATCCTCCACT 
GTCTCTTCTA CAGAATCAAC AACAGAATCT TCAACAGATT CTTCTACTGA ATCGTCCACA 
GATTCTTCCA CCTTCTCTTC TACGGATTCC TCTACGGAAT CATCTACTGC AGCAATCACC 
ACCACAGAAC CAGGTTCTTC TTCCACAGAG CCTGGAACAA CCACCACAGA TTCCCCAACA 
ACCACAACGC CCAAACCGTC TGGTGGAGTT TGCGAGGGCA AAACGGACGA CTCTTTGGTG 
CCGTATCCCA ACAACTGCAG CAAATTTATC ATGTGTCAAT ACCCGATTCC TGTGGGCTTT 
GATTGTCCCG AGGGTTTGGA GTTTAGTCCC ACGGAATTGC AGTGCATGGA TCCCGATTTG 
GCAAATTGTA GTCCGAGTGT GACAACACCT GCAATACCAA CTTCTACGAC TTCAGAAGCT 
TCAACAACTG AATCTACAAC GGATTCTACT ACTAAAGAAC CTTCAATAAC GGACTCTACA 
ACAAAAGAGC CTTCAACAAC AGATTCTACA ACGAAATCTA CAACAACTGA GCCAACAACA 
GAGTCCACAA CAACTGAACC TTCAACAACA GTGTCTACAA CGGAGTTTTC GACAACAGTT 
TCTACGACAG AATCTACAAC TAAGGAACCT TCAACAACAG AGTCTACAAC GGAGTCTACA 
ACGCAGTCTA CAACGGAGTC TACAACGGAT TCTACTACTG AGTCTTCAAC AACAGATTCT 
ACAACTAAAG AACCTTCAAC AACAGAGTCT ACAACGGAAT CGACAACAGA TTCTACAACA 
GTTTCCACGA CAGAGTCTAC AACTAAAGAA CCTTCAACAA CAGAGTCGAC CACAACTGAG 
CCTTCAACAA CAGATTCTAC AACGAAATCT ACAACAACTG AGCCAACAAC AGAGTCCACA 
ACAACTGAAC CTTCCACAAC AGTGTCTACA ACTGAGGAAC CTTCAACCAC AGTGTCCACA 
ACGGAATCTA CAACGGAGTC TACAACCAAG GAACCTTCCA CAACAGAGTC CAGTACGCCA 
GAGCCCTCGA CCACCCCCAA GGAGCCAACT ACTTTGGATC CCTACACTCC CAACATTTGC 
TGTGGCTATC CTCTGGGAAC TCTTTTGCCT TATCCCAACG ATTGCATGCG ATATGTCGTG 
TGTGATTATC CCATTCCTTA TGCGGTGGAT TGCGATCCGG GAACCTTCTT TGATGACAGG 
CTGCTGCAAT GTACATCAAT TCCCACTCAA ACCTGCATTT ATAAAAGAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001353760.3
CDS	1..3894
Translation	MVGLSPPPSHSVYQTLDSSQQIKLKNCPHQRSSDDSGGINKVNLLTWLLLMVALVWSWQFNGGVAMSQAEPRHKTCDNEQRYENQVMCEGKEFGALVPYPDNCSLFLVCDCLYPTVKLCPAELWWDNASQICNYPQSVDCVYYTIETPEPTDGTTRITPVITTESTTLSTPKVTEYPTTLSTPKVTEHPTTLSTPKVTEYPTTPKVTDVPCITPTSSWDPPPAPPGIPDDFCRHQSDGSVHPYPYDCQAYINCTHGWPVLNYCIEDKVFNPWLFICDTHDTTECEEEPLPTATPTTTEMVPTTTTTELAPTSTTEADDVECGPTPEFVEEDYCQPLGNGFYEYPYNCSAYLLCRNGCTDLDYCISDKLFNPWLHICDTPDSVYCDPLPYPTAPPSSTTPSSTTTSVDVSTTESTTATTEAATSTTPGLPPDVNPNECRGVPDGTRLPYAGNCSQFLICKDDQVEMGQCPPLTLFNPDLLICDEADDVLCYGDRTTTPIPTTTTTEATTPTPTTTTTKATTLGPEELCEGQSLGASFAYPQDCSKYYLCLGGGQWFLTDCIYGSYFDPNTMNCGPNVSPTACQEGQATTTTAATTTTQKVTTPTTTTEEPSTPAATTTTQAPPGGICGGRDEGENIAYPKDCNKYIVCVSPIPIAFYCPAGSYFSSKLQNCASWEESDCDQDASTTTLSPGLTQAPLEPTMCTNSSRDTFPYPDNCQWFIRCVDDYIYMMDVCNCGEYYDPISGKCGADVPSDACRWDYTSTTSTTSEPTTSTAVTRPPPQKGPCDDVADGALVPYPNDCTKYIKCDRPIATAFDCEEGDEFSVALGECVDASLAGCSVTTTTNPSGSTTPSIPSTKTTEGDSTTDSSTASSTESSTESSTVSSTESTTESSTDSSTESSTDSSTFSSTDSSTESSTAAITTTEPGSSSTEPGTTTTDSPTTTTPKPSGGVCEGKTDDSLVPYPNNCSKFIMCQYPIPVGFDCPEGLEFSPTELQCMDPDLANCSPSVTTPAIPTSTTSEASTTESTTDSTTKEPSITDSTTKEPSTTDSTTKSTTTEPTTESTTTEPSTTVSTTEFSTTVSTTESTTKEPSTTESTTESTTQSTTESTTDSTTESSTTDSTTKEPSTTESTTESTTDSTTVSTTESTTKEPSTTESTTTEPSTTDSTTKSTTTEPTTESTTTEPSTTVSTTEEPSTTVSTTESTTESTTKEPSTTESSTPEPSTTPKEPTTLDPYTPNICCGYPLGTLLPYPNDCMRYVVCDYPIPYAVDCDPGTFFDDRLLQCTSIPTQTCIYKRA

Target ORF information:

RefSeq Version	XM_001353724.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA18465), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001353724.3

ORF Insert Sequence:

ATGGTTGGCC TCAGTCCTCC CCCGTCGCAC AGCGTCTATC AAACGCTTGA CAGCAGCCAA 
CAGATCAAGC TGAAAAATTG TCCCCACCAA AGGAGCTCTG ACGACTCTGG AGGAATTAAT 
AAAGTCAATC TCTTGACATG GCTCCTGCTG ATGGTAGCTC TCGTCTGGAG CTGGCAATTC 
AATGGAGGCG TGGCCATGTC ACAGGCGGAA CCGCGCCACA AGACCTGCGA CAATGAGCAG 
CGATACGAGA ACCAGGTCAT GTGCGAGGGC AAAGAGTTTG GCGCCCTGGT GCCGTATCCG 
GATAACTGTA GTCTGTTTCT CGTCTGCGAT TGTCTGTATC CTACGGTCAA GCTCTGTCCG 
GCAGAGCTCT GGTGGGATAA TGCCTCACAG ATATGCAACT ATCCGCAGTC GGTGGATTGT 
GTCTACTACA CCATTGAAAC GCCGGAACCG ACGGATGGAA CAACGAGAAT TACGCCGGTT 
ATTACCACCG AATCAACGAC TTTGAGTACA CCAAAAGTCA CCGAATACCC AACGACATTG 
AGCACGCCAA AAGTCACCGA ACACCCAACG ACATTGAGCA CGCCAAAAGT CACCGAATAC 
CCAACGACTC CAAAAGTGAC GGACGTTCCC TGTATCACGC CCACTTCCAG CTGGGACCCG 
CCACCAGCAC CGCCTGGCAT TCCCGATGAT TTCTGTAGGC ATCAATCGGA TGGCTCGGTG 
CATCCTTATC CGTACGATTG CCAGGCGTAT ATCAACTGTA CCCATGGCTG GCCCGTCCTC 
AACTATTGCA TCGAGGATAA GGTCTTCAAT CCGTGGCTGT TCATCTGCGA TACCCACGAC 
ACAACCGAAT GCGAGGAGGA GCCGTTGCCC ACGGCCACGC CCACCACTAC AGAGATGGTT 
CCCACTACCA CCACAACAGA GTTGGCTCCC ACTTCCACCA CAGAAGCGGA CGATGTAGAG 
TGCGGACCCA CACCAGAGTT CGTGGAGGAG GATTACTGCC AGCCCTTGGG CAATGGCTTC 
TACGAGTATC CCTACAATTG CAGTGCGTAC CTCTTGTGCC GCAATGGCTG CACGGACTTG 
GACTACTGCA TATCGGATAA GTTGTTCAAT CCATGGCTCC ACATTTGCGA TACTCCCGAC 
TCAGTGTACT GCGATCCACT GCCTTATCCC ACTGCTCCAC CGTCTTCCAC GACTCCTTCG 
AGCACCACAA CTTCTGTGGA TGTTTCTACG ACGGAGAGCA CCACTGCCAC CACAGAGGCA 
GCCACAAGCA CAACGCCGGG ACTGCCCCCC GATGTGAATC CCAACGAATG TCGCGGTGTT 
CCCGATGGCA CTCGATTGCC ATATGCCGGC AACTGTTCGC AGTTCTTAAT CTGCAAGGAC 
GATCAGGTGG AGATGGGTCA GTGTCCTCCA TTGACGCTCT TTAATCCGGA TTTACTCATC 
TGCGATGAGG CTGATGATGT CTTGTGCTAT GGGGATCGCA CCACCACACC GATACCCACG 
ACAACAACCA CTGAGGCGAC AACACCGACG CCTACCACAA CGACAACGAA GGCCACAACT 
CTGGGGCCCG AAGAGCTCTG TGAGGGGCAG AGTCTGGGAG CCTCCTTCGC GTATCCCCAG 
GACTGCTCTA AGTACTATTT GTGTTTGGGA GGTGGACAAT GGTTCCTGAC AGACTGCATT 
TATGGCAGCT ACTTTGACCC CAACACAATG AATTGTGGGC CCAATGTCTC ACCAACGGCC 
TGCCAAGAGG GACAAGCAAC CACAACAACG GCAGCAACCA CAACCACCCA GAAGGTAACC 
ACTCCAACAA CGACAACAGA GGAACCATCG ACCCCGGCGG CAACGACCAC AACTCAAGCG 
CCACCGGGTG GCATTTGCGG TGGCCGGGAT GAAGGTGAAA ATATCGCCTA TCCCAAGGAT 
TGCAACAAGT ACATCGTCTG CGTCTCCCCC ATACCCATTG CCTTCTACTG CCCAGCGGGA 
TCCTACTTCA GTTCGAAGCT CCAGAACTGC GCCAGCTGGG AGGAGAGTGA CTGCGATCAG 
GATGCCAGCA CTACAACGCT ATCCCCAGGG TTAACCCAAG CGCCGCTGGA GCCAACGATG 
TGCACAAACA GCAGTCGCGA TACCTTCCCG TATCCGGACA ACTGTCAGTG GTTCATCCGC 
TGTGTAGACG ACTACATCTA CATGATGGAC GTCTGCAACT GCGGGGAGTA CTACGATCCC 
ATAAGCGGCA AGTGCGGAGC AGATGTTCCA TCGGATGCCT GTCGCTGGGA CTACACTTCG 
ACCACGTCCA CCACCAGCGA ACCGACCACC AGCACAGCCG TGACCAGACC CCCACCGCAG 
AAGGGTCCCT GCGACGATGT GGCGGATGGT GCTTTGGTGC CATATCCTAA CGATTGCACC 
AAGTACATCA AATGCGATCG CCCAATAGCC ACGGCCTTCG ACTGCGAAGA GGGGGATGAG 
TTCAGTGTCG CGCTGGGGGA GTGTGTGGAC GCTTCTCTGG CCGGTTGCTC GGTTACAACC 
ACGACCAACC CTTCGGGATC GACGACGCCA TCGATACCAT CGACAAAGAC AACAGAAGGG 
GACTCTACAA CAGATTCTTC CACAGCATCA TCCACGGAAT CATCTACTGA ATCCTCCACT 
GTCTCTTCTA CAGAATCAAC AACAGAATCT TCAACAGATT CTTCTACTGA ATCGTCCACA 
GATTCTTCCA CCTTCTCTTC TACGGATTCC TCTACGGAAT CATCTACTGC AGCAATCACC 
ACCACAGAAC CAGGTTCTTC TTCCACAGAG CCTGGAACAA CCACCACAGA TTCCCCAACA 
ACCACAACGC CCAAACCGTC TGGTGGAGTT TGCGAGGGCA AAACGGACGA CTCTTTGGTG 
CCGTATCCCA ACAACTGCAG CAAATTTATC ATGTGTCAAT ACCCGATTCC TGTGGGCTTT 
GATTGTCCCG AGGGTTTGGA GTTTAGTCCC ACGGAATTGC AGTGCATGGA TCCCGATTTG 
GCAAATTGTA GTCCGAGTGT GACAACACCT GCAATACCAA CTTCTACGAC TTCAGAAGCT 
TCAACAACTG AATCTACAAC GGATTCTACT ACTAAAGAAC CTTCAATAAC GGACTCTACA 
ACAAAAGAGC CTTCAACAAC AGATTCTACA ACGAAATCTA CAACAACTGA GCCAACAACA 
GAGTCCACAA CAACTGAACC TTCAACAACA GTGTCTACAA CGGAGTTTTC GACAACAGTT 
TCTACGACAG AATCTACAAC TAAGGAACCT TCAACAACAG AGTCTACAAC GGAGTCTACA 
ACGCAGTCTA CAACGGAGTC TACAACGGAT TCTACTACTG AGTCTTCAAC AACAGATTCT 
ACAACTAAAG AACCTTCAAC AACAGAGTCT ACAACGGAAT CGACAACAGA TTCTACAACA 
GTTTCCACGA CAGAGTCTAC AACTAAAGAA CCTTCAACAA CAGAGTCGAC CACAACTGAG 
CCTTCAACAA CAGATTCTAC AACGAAATCT ACAACAACTG AGCCAACAAC AGAGTCCACA 
ACAACTGAAC CTTCCACAAC AGTGTCTACA ACTGAGGAAC CTTCAACCAC AGTGTCCACA 
ACGGAATCTA CAACGGAGTC TACAACCAAG GAACCTTCCA CAACAGAGTC CAGTACGCCA 
GAGCCCTCGA CCACCCCCAA GGAGCCAACT ACTTTGGATC CCTACACTCC CAACATTTGC 
TGTGGCTATC CTCTGGGAAC TCTTTTGCCT TATCCCAACG ATTGCATGCG ATATGTCGTG 
TGTGATTATC CCATTCCTTA TGCGGTGGAT TGCGATCCGG GAACCTTCTT TGATGACAGG 
CTGCTGCAAT GTACATCAAT TCCCACTCAA ACCTGCATTT ATAAAAGAGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA18465 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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