Dpse\GA15661 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA15661
Entrez Gene ID	4804821
Full Name	GA15661 gene product from transcript GA15661-RG
Synonyms	GA15661,dpse_GLEANR_14150
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015184739.1	XP_015040225.1	uncharacterized protein, isoform G
XM_004444252.2	XP_004444309.1	uncharacterized protein, isoform C
XM_001361286.3	XP_001361323.1	uncharacterized protein, isoform F
XM_015184738.1	XP_015040224.1	uncharacterized protein, isoform D
XM_004444251.2	XP_004444308.1	uncharacterized protein, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA15661 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA15661 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe03970	XM_004444252.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA15661), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe03971	XM_001361286.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA15661), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe03972	XM_015184738.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA15661), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe03972	XM_004444251.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA15661), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe23190	XM_015184739.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA15661), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA15661 ( XM_004444252.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_004444309.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA15661), mRNA.

Price & Availability↑

CloneID	ODe03970
Clone ID Related Accession (Same CDS sequence)	XM_004444252.2
Accession Version	XM_004444252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_004444252.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC CCATTGACGA GAGTGTGCTG AACGCACTGA AGGGGGTGAC CTCCAAGCAG 
ACCCGCCTGC CCAAGCCGCT GCTCATCAAG ATCTATGTCG CCAGCCAGAA GCAGGAGTTC 
AACCAGGAGC GCCGCATGCT CCTGGAGCTG GTGGGCCCCG AGCTGCAGGG CCTCTACGAC 
GACCGCCAAA TCGAGTTGGA GTTCGTGGAC ATGCACTTCG GCACTGGGGC GCTGGAGGTG 
AACCAGCTGG AGCGGGATCC CTACCTGCTG CAGGACTACC TGCACGAGAT CGACACCTGT 
CATGCCCACA CGAAGAGCGT CTTCTTTCTG GCGCTCATCG GCGACGGCCT CGGACGCCTG 
CCGCTGCCCA CGCGCCTGGA CGAGGACATC TATGCCGCGG TGCTGGCCGA CCCTCAGACA 
ACGGCCGACC AGGAGGCTCT TCTAGCCAGG TGGTACGAGA TGGATCCATC CAAGGTGCAG 
CGTCAGCTGA AGCAGGACTA TCGAGTACTG CCCATGGACG AGTGGCTCAG CGAGTCCCAG 
CAGATGCAAA AGATGCTGGA GCAGGCGCTG CAAGGCTTTG TCCAAGGACC AGGTGCCTCG 
GCGCGATCTG CAGACTTCCT GAAGCGGGTG CAGCAGCTGA GGCGCACGCA GATCGAACGC 
GAAGTGGAGC AGGCCATGGC CCTCTCCAGC GAGAAGATCC TCGCCGTGTT TCGGGAACGG 
GCCGCCCAGT GCAGCAAGCG GGATGTGGAG GCCAATGAGC GGCTTCGGAA GATCAAGGAC 
GAGCTCACCA TGAACCTCTC CACGGACAAT CACACAACCC TAGTGGTGCC AGGCAGTGCC 
TCGTGCGAAA TGATCGACCC GGACAACGAG GACCACGAGT CCTATCTCAG CAAATTCAAG 
AACAAAGTCA CCGACAAGCT GCGTCTGCTA ATCGAGGCGC ACATCACCAA TGATCCCGAT 
GTAATCAAGG GGAGGAAAAA GACTGTTCAG GAAATCTTTC ACGAGCACGC CACTCACCTG 
CGCATCCTGC GCGATCATGT GGAGGGGGCG GCCCTCATCG AGTCGCGGGT GCCACCGCAG 
CTGCGCCAGA ACCTGATGGC GAACTTCAGG AACGGCAGCC GGCATGCGCC GTACTTCATT 
TGCGGCTCGG ATGGCAGCGG CAAGAGCGCC CTGATCAGCA CCCTGTACGG GCAGGTGGCC 
AGCTGGTTCG TCTCGACGAA GGTCCACCGC GTGATCCGCT TCGCGAAGGC CACGCCGCGC 
TCGGCCTACA ACCTGGAGCT GCTGCGCGTG ATCTGCCAGC AGATATCCAT CATCTTCAAC 
ATCCCCGAGG GCTACCTACC CAAGGACGCC TCCTTCGATC CGCTGTACAT CAGCACCTGG 
TTCCAGAACC TGCTGCGGCG CGTCGAGGAC ATGAGCAACG ACGTGCTGTT CCTGTTCATC 
GACGACCTGC ACCTGCTGAA CCCCCTGGAC TGCGACATCG TGACCGCCCT CTCCTGGCTG 
CCCACCTCGC TGCCCTGGAA CGTGCAGATC ATCTGCAGCT CCACCACCCC CGTCGAGCAG 
CTCAAGTTCA CGCCCATGCA GCGGGACCGC TTCAAGTCGG CGGAGTACCA GTTCGACCTC 
AACCTGGGCG AGTACGCCAC GGAGCTGCGG CGACCTCCGC AGTGCGTCTC GGACGTCAGC 
TTCGTCCTGT ACGTGGAGCA GCAGTTCGAC GGCCTGGAGC GCCACTACGG CAGGCAGGCG 
GTGGGCAGCC TGGCCGCCTA CATCACCTGC TCCGAGTACG GGCTGAGCGA GACGGAGCTC 
CTGGAGCTGC TCATGCCCAC CGACGATCCC GAGTCCCTGA TCGACACCAG AAGCGGACAC 
TTTAGCTTCG CCACCTTCAA GAAGATCCAC AGGGAAATGG ACAAGCTCCT GCTGCTGCAC 
GACAAGATCA TGTCCGGCAA GGTGCTCATC CAGTGGCGCC ACAACTACTG CGCCGTGGTG 
GCCAAGCGAC GGTACATGGG CGGCCAGAGG ACGCGCAACC TGCACATGGA GCTGGCCAAC 
CTGTTCTTCC CGCAGGACGA GGACGAGAGC ACGCTGGAGA ACGAGTCCAC CCAGAGCGAG 
AACAAGTCCG TGATTAGCCT CAAGGACACG CTCAGGACAG TCTGCGACCT GCCGCACAAG 
GACAGGGAGC GCGAGAAGGA CAACGTCTCC GCTGGCCGCA AGTCCTCCTC CACCCACCAC 
AACGACGACA CCTCCACCTT CTACAACCCC ATCGCCGCCG ACGTCAGCTA CAGCATGCGG 
CACGTGGAGG AGAGCTGGCA CCACCTGATG CGCTCCGACG ACACGACACG CTTCAAGCAA 
ATCGCAGTCT GCAACTTTGA TTTCCTGCTG GCGGCGGTAC AAACTGTGTC GATCAGCTAT 
TTGCGCTGCC TGATCGAGCA CGTCCGCTGC TATATCCTCG ACAGGGACAT CGAGCTGATC 
TACTACACGA TCCGCAAGTC CAGCGACGTG CTCACGCGCG ACCCCATGCA GCTGGGCTCC 
CAGCTGATCT CCTGGCTGCG TCCGATCAGC GAGCACGACG ACGACGACAA CTCGCTGCTC 
AGCATGACGG TGCGCTCGGC GACGGCCTGG TGCGATGGCT ACGCCGTGCC GCTGCTGGTG 
CCGCTCACGG GATGGCTGCC GGCACCGCTG CCCTCGCAGA TCCGCACCAT GACGGTGTCG 
GGCACGGGCG TCATCCGAGC CGTCTGCCTG GCGCCCTCGA GGCAGCACCT CATCCTGGCC 
ACCAGCTCGG GCGAGGTGCA GCAGTGGCAC ATCATGAGCA ACTCGCTGGA ACACATCTTC 
AAGGGACACA CTGCGGCAGT CACGTGCCTG CTGGTGGCTC CGACCAGCGA GCCGGAGCTC 
CTCCTCACCG GCTCCGAGGA CACCAGCGTG CTCGTCTGGC ACGTCTCGCA GCGCGACCGT 
CGGGCGCACA TCAACGCCCA CACAGCCCCC ATAACGGGCG TGGCGGCCGG GGTCAATAAC 
ACGCTGATCA TCAGCAGCAG CGAGGATGCC ACAATCGCCA TCACGGACCT GGCCAGCGGC 
AAACTGAAAC ACCGAATCAG CCACCATCGC GGCCCTGTGA CCGGGATCCT GGTGGCCGGA 
GCGTGCGATG TCCTGATCTC GGGCAGCCAC GACCGAACCA TCTGCGTGTG GAACCTCGAG 
AACTTTGCGC TGCTGAACAC CATGCAAATG CTGAGCCCCG TCCTGCGCAT CGACATCTCA 
TGGAACTCGG TATTCCTCCT GGCCCTGTGC GAGGACAATG CCCTCTATGT GCGCACTCTG 
GCCACGGGCA AGGAGCTGCA CACGCTCAAG GGCCACAAGT CAAAGATACG GACCATTTCC 
ATTGGCAAGG ACAGTCAGCG CTGCGTGGTG GGATGCGACG ATACGCGAGC CCTGATCTAC 
GACATGCACT CCGGCAAGCT GGTGCGCTCC ATGCCGCCCA ACCCGGGGCC GGTGACCGCC 
GTCTATGCGA TGGAGAACGA CGACTTCCTG GTCACGGTGG GCGGCAACAA GATCACGTTC 
TACTCGTTCC GCAACGAGGA GCTCTACGTG AACCCGTACT CCCGCAACCC GCGCCGCAAG 
CGCAGTCTGA AGCGGCACGC CCAGGCCCAG CGGTCGCCCT CGACGACCCT GCCACCGATC 
ACGTGCTTCG ACCTCTCCCG CGACTCGCAG CTGATGGCCA TCGCCTCAGG CCGCAGCGTG 
CACCTGATGC GCATCAACAC GCCGGAGTAC CAGTGCAGCC TGGAGGGGCA CGCGGGCGTG 
GTCAACTGCC TGAAGTTCGC GCCCAACGGA GAGTTCCTCA CCACGGGATC CGACGATCGG 
CTCGTCCAGG TGTGGAACCT GGCGGCCACC GAGACCATTA ACACCTTCAA GGGGCATGCG 
GCGCCGGTGA TCAAGGTGGT GGTGCTGATG GACTCGCTGC GGGTGATCTC CACGGACCGG 
GACTCACTGC TGCTCGTGTG GATGGCCAGC TCGGGCAACC TGCTGCAGAC CATCCAGGGA 
CCCTACAAGA GCCTGGTGGT GACCAACAAC ATGCGCTTCG CCGCCTCCAG CAACGGGGAC 
AACACTCTGA AGATCTGGTC CCTCACCCAG GAGGACGAGA AGTACTCGGT CTCGCACTCG 
GACGAGATCA CCTGCTTCGA GATCAGCGCC GACAGCATGC ACATCATCAC GGGCTCCCGG 
GACATGTCGC TGAAGGTGTG GCAGTCCACG GGCGGCAAGC TCAGCCAGGT GCTGGTCGGG 
CACAGCGACG CTGTGACCTG TGTGGCCGTC TCGGTGACCA ACAAGACGCA GGTCCTCTCC 
GGCTCCAAGG ACACGAACCT CATCCTGTGG GACCTGCTCA CTGGCGAGGA GGTTCACACG 
CTGGCCGGAC ACTTGGGTCC AGTGATCGGA GTGAAGGTTT CGGCAGATGG CTCCACGGCT 
GTGTCGGGCA GCGATGACAA GACCTTGATC GTGTGGGAGA CGAAGCGCGG ATTGGCCCTG 
ACCTCGCTGC AGATGCACGT GCCCTTCACC CACTTCGACA TCAGCCTGGA GGTGTCGCGG 
GTCCTGGTCC AACTGGTGGA CAGCTACAAC CTCCCGGTGA TCTGTCTGCA CAACACCCCG 
GCGCAGTATG TAAAGCTGCC CACGTACTCG GGACCCACCA AGGATGTGGA TGATCTCCGG 
CCACAGGGCC CGAAGCGCCA GATGAAGCGC CTGCTGAAGA AGGAGGTCTC CCTGGACACG 
TACACTTGGC AGAAGAAGTA CGGCCACCTG ACCTCGTCGG TCATGATGGC CCAGGTTGAC 
GAGCGCCTGA AGCGACGCTT CAGCGTCTCG GCCAGCATGG AGGAGATCTC GAAGATCGCC 
GAGAACAAGA CAGGGGCCTC CAATGCCAAC ATCGGCCCCG AGCAGGCCGC TCTGGCCCAG 
TCGCAGCACT TCGACCAGCT GGAGGCGCTG TGGAACAAGC GGTCTCCGCC CCGACGCCGC 
CACAACGCGG GTCTGTCGAG GCAGACATCG CTGGTGGAGG ACCGGCTCGA GTCGTCGGAC 
GACGACGAGT ACCAGGACGA GCGCGCAGAT CACTTCGCTT CAGGAGGAGG AGGATACCAC 
AAGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004444309.1
CDS	550..5658
Translation	MAAPIDESVLNALKGVTSKQTRLPKPLLIKIYVASQKQEFNQERRMLLELVGPELQGLYDDRQIELEFVDMHFGTGALEVNQLERDPYLLQDYLHEIDTCHAHTKSVFFLALIGDGLGRLPLPTRLDEDIYAAVLADPQTTADQEALLARWYEMDPSKVQRQLKQDYRVLPMDEWLSESQQMQKMLEQALQGFVQGPGASARSADFLKRVQQLRRTQIEREVEQAMALSSEKILAVFRERAAQCSKRDVEANERLRKIKDELTMNLSTDNHTTLVVPGSASCEMIDPDNEDHESYLSKFKNKVTDKLRLLIEAHITNDPDVIKGRKKTVQEIFHEHATHLRILRDHVEGAALIESRVPPQLRQNLMANFRNGSRHAPYFICGSDGSGKSALISTLYGQVASWFVSTKVHRVIRFAKATPRSAYNLELLRVICQQISIIFNIPEGYLPKDASFDPLYISTWFQNLLRRVEDMSNDVLFLFIDDLHLLNPLDCDIVTALSWLPTSLPWNVQIICSSTTPVEQLKFTPMQRDRFKSAEYQFDLNLGEYATELRRPPQCVSDVSFVLYVEQQFDGLERHYGRQAVGSLAAYITCSEYGLSETELLELLMPTDDPESLIDTRSGHFSFATFKKIHREMDKLLLLHDKIMSGKVLIQWRHNYCAVVAKRRYMGGQRTRNLHMELANLFFPQDEDESTLENESTQSENKSVISLKDTLRTVCDLPHKDREREKDNVSAGRKSSSTHHNDDTSTFYNPIAADVSYSMRHVEESWHHLMRSDDTTRFKQIAVCNFDFLLAAVQTVSISYLRCLIEHVRCYILDRDIELIYYTIRKSSDVLTRDPMQLGSQLISWLRPISEHDDDDNSLLSMTVRSATAWCDGYAVPLLVPLTGWLPAPLPSQIRTMTVSGTGVIRAVCLAPSRQHLILATSSGEVQQWHIMSNSLEHIFKGHTAAVTCLLVAPTSEPELLLTGSEDTSVLVWHVSQRDRRAHINAHTAPITGVAAGVNNTLIISSSEDATIAITDLASGKLKHRISHHRGPVTGILVAGACDVLISGSHDRTICVWNLENFALLNTMQMLSPVLRIDISWNSVFLLALCEDNALYVRTLATGKELHTLKGHKSKIRTISIGKDSQRCVVGCDDTRALIYDMHSGKLVRSMPPNPGPVTAVYAMENDDFLVTVGGNKITFYSFRNEELYVNPYSRNPRRKRSLKRHAQAQRSPSTTLPPITCFDLSRDSQLMAIASGRSVHLMRINTPEYQCSLEGHAGVVNCLKFAPNGEFLTTGSDDRLVQVWNLAATETINTFKGHAAPVIKVVVLMDSLRVISTDRDSLLLVWMASSGNLLQTIQGPYKSLVVTNNMRFAASSNGDNTLKIWSLTQEDEKYSVSHSDEITCFEISADSMHIITGSRDMSLKVWQSTGGKLSQVLVGHSDAVTCVAVSVTNKTQVLSGSKDTNLILWDLLTGEEVHTLAGHLGPVIGVKVSADGSTAVSGSDDKTLIVWETKRGLALTSLQMHVPFTHFDISLEVSRVLVQLVDSYNLPVICLHNTPAQYVKLPTYSGPTKDVDDLRPQGPKRQMKRLLKKEVSLDTYTWQKKYGHLTSSVMMAQVDERLKRRFSVSASMEEISKIAENKTGASNANIGPEQAALAQSQHFDQLEALWNKRSPPRRRHNAGLSRQTSLVEDRLESSDDDEYQDERADHFASGGGGYHKG

Target ORF information:

RefSeq Version	XM_004444252.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA15661), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004444252.2

ORF Insert Sequence:

ATGGCCGCGC CCATTGACGA GAGTGTGCTG AACGCACTGA AGGGGGTGAC CTCCAAGCAG 
ACCCGCCTGC CCAAGCCGCT GCTCATCAAG ATCTATGTCG CCAGCCAGAA GCAGGAGTTC 
AACCAGGAGC GCCGCATGCT CCTGGAGCTG GTGGGCCCCG AGCTGCAGGG CCTCTACGAC 
GACCGCCAAA TCGAGTTGGA GTTCGTGGAC ATGCACTTCG GCACTGGGGC GCTGGAGGTG 
AACCAGCTGG AGCGGGATCC CTACCTGCTG CAGGACTACC TGCACGAGAT CGACACCTGT 
CATGCCCACA CGAAGAGCGT CTTCTTTCTG GCGCTCATCG GCGACGGCCT CGGACGCCTG 
CCGCTGCCCA CGCGCCTGGA CGAGGACATC TATGCCGCGG TGCTGGCCGA CCCTCAGACA 
ACGGCCGACC AGGAGGCTCT TCTAGCCAGG TGGTACGAGA TGGATCCATC CAAGGTGCAG 
CGTCAGCTGA AGCAGGACTA TCGAGTACTG CCCATGGACG AGTGGCTCAG CGAGTCCCAG 
CAGATGCAAA AGATGCTGGA GCAGGCGCTG CAAGGCTTTG TCCAAGGACC AGGTGCCTCG 
GCGCGATCTG CAGACTTCCT GAAGCGGGTG CAGCAGCTGA GGCGCACGCA GATCGAACGC 
GAAGTGGAGC AGGCCATGGC CCTCTCCAGC GAGAAGATCC TCGCCGTGTT TCGGGAACGG 
GCCGCCCAGT GCAGCAAGCG GGATGTGGAG GCCAATGAGC GGCTTCGGAA GATCAAGGAC 
GAGCTCACCA TGAACCTCTC CACGGACAAT CACACAACCC TAGTGGTGCC AGGCAGTGCC 
TCGTGCGAAA TGATCGACCC GGACAACGAG GACCACGAGT CCTATCTCAG CAAATTCAAG 
AACAAAGTCA CCGACAAGCT GCGTCTGCTA ATCGAGGCGC ACATCACCAA TGATCCCGAT 
GTAATCAAGG GGAGGAAAAA GACTGTTCAG GAAATCTTTC ACGAGCACGC CACTCACCTG 
CGCATCCTGC GCGATCATGT GGAGGGGGCG GCCCTCATCG AGTCGCGGGT GCCACCGCAG 
CTGCGCCAGA ACCTGATGGC GAACTTCAGG AACGGCAGCC GGCATGCGCC GTACTTCATT 
TGCGGCTCGG ATGGCAGCGG CAAGAGCGCC CTGATCAGCA CCCTGTACGG GCAGGTGGCC 
AGCTGGTTCG TCTCGACGAA GGTCCACCGC GTGATCCGCT TCGCGAAGGC CACGCCGCGC 
TCGGCCTACA ACCTGGAGCT GCTGCGCGTG ATCTGCCAGC AGATATCCAT CATCTTCAAC 
ATCCCCGAGG GCTACCTACC CAAGGACGCC TCCTTCGATC CGCTGTACAT CAGCACCTGG 
TTCCAGAACC TGCTGCGGCG CGTCGAGGAC ATGAGCAACG ACGTGCTGTT CCTGTTCATC 
GACGACCTGC ACCTGCTGAA CCCCCTGGAC TGCGACATCG TGACCGCCCT CTCCTGGCTG 
CCCACCTCGC TGCCCTGGAA CGTGCAGATC ATCTGCAGCT CCACCACCCC CGTCGAGCAG 
CTCAAGTTCA CGCCCATGCA GCGGGACCGC TTCAAGTCGG CGGAGTACCA GTTCGACCTC 
AACCTGGGCG AGTACGCCAC GGAGCTGCGG CGACCTCCGC AGTGCGTCTC GGACGTCAGC 
TTCGTCCTGT ACGTGGAGCA GCAGTTCGAC GGCCTGGAGC GCCACTACGG CAGGCAGGCG 
GTGGGCAGCC TGGCCGCCTA CATCACCTGC TCCGAGTACG GGCTGAGCGA GACGGAGCTC 
CTGGAGCTGC TCATGCCCAC CGACGATCCC GAGTCCCTGA TCGACACCAG AAGCGGACAC 
TTTAGCTTCG CCACCTTCAA GAAGATCCAC AGGGAAATGG ACAAGCTCCT GCTGCTGCAC 
GACAAGATCA TGTCCGGCAA GGTGCTCATC CAGTGGCGCC ACAACTACTG CGCCGTGGTG 
GCCAAGCGAC GGTACATGGG CGGCCAGAGG ACGCGCAACC TGCACATGGA GCTGGCCAAC 
CTGTTCTTCC CGCAGGACGA GGACGAGAGC ACGCTGGAGA ACGAGTCCAC CCAGAGCGAG 
AACAAGTCCG TGATTAGCCT CAAGGACACG CTCAGGACAG TCTGCGACCT GCCGCACAAG 
GACAGGGAGC GCGAGAAGGA CAACGTCTCC GCTGGCCGCA AGTCCTCCTC CACCCACCAC 
AACGACGACA CCTCCACCTT CTACAACCCC ATCGCCGCCG ACGTCAGCTA CAGCATGCGG 
CACGTGGAGG AGAGCTGGCA CCACCTGATG CGCTCCGACG ACACGACACG CTTCAAGCAA 
ATCGCAGTCT GCAACTTTGA TTTCCTGCTG GCGGCGGTAC AAACTGTGTC GATCAGCTAT 
TTGCGCTGCC TGATCGAGCA CGTCCGCTGC TATATCCTCG ACAGGGACAT CGAGCTGATC 
TACTACACGA TCCGCAAGTC CAGCGACGTG CTCACGCGCG ACCCCATGCA GCTGGGCTCC 
CAGCTGATCT CCTGGCTGCG TCCGATCAGC GAGCACGACG ACGACGACAA CTCGCTGCTC 
AGCATGACGG TGCGCTCGGC GACGGCCTGG TGCGATGGCT ACGCCGTGCC GCTGCTGGTG 
CCGCTCACGG GATGGCTGCC GGCACCGCTG CCCTCGCAGA TCCGCACCAT GACGGTGTCG 
GGCACGGGCG TCATCCGAGC CGTCTGCCTG GCGCCCTCGA GGCAGCACCT CATCCTGGCC 
ACCAGCTCGG GCGAGGTGCA GCAGTGGCAC ATCATGAGCA ACTCGCTGGA ACACATCTTC 
AAGGGACACA CTGCGGCAGT CACGTGCCTG CTGGTGGCTC CGACCAGCGA GCCGGAGCTC 
CTCCTCACCG GCTCCGAGGA CACCAGCGTG CTCGTCTGGC ACGTCTCGCA GCGCGACCGT 
CGGGCGCACA TCAACGCCCA CACAGCCCCC ATAACGGGCG TGGCGGCCGG GGTCAATAAC 
ACGCTGATCA TCAGCAGCAG CGAGGATGCC ACAATCGCCA TCACGGACCT GGCCAGCGGC 
AAACTGAAAC ACCGAATCAG CCACCATCGC GGCCCTGTGA CCGGGATCCT GGTGGCCGGA 
GCGTGCGATG TCCTGATCTC GGGCAGCCAC GACCGAACCA TCTGCGTGTG GAACCTCGAG 
AACTTTGCGC TGCTGAACAC CATGCAAATG CTGAGCCCCG TCCTGCGCAT CGACATCTCA 
TGGAACTCGG TATTCCTCCT GGCCCTGTGC GAGGACAATG CCCTCTATGT GCGCACTCTG 
GCCACGGGCA AGGAGCTGCA CACGCTCAAG GGCCACAAGT CAAAGATACG GACCATTTCC 
ATTGGCAAGG ACAGTCAGCG CTGCGTGGTG GGATGCGACG ATACGCGAGC CCTGATCTAC 
GACATGCACT CCGGCAAGCT GGTGCGCTCC ATGCCGCCCA ACCCGGGGCC GGTGACCGCC 
GTCTATGCGA TGGAGAACGA CGACTTCCTG GTCACGGTGG GCGGCAACAA GATCACGTTC 
TACTCGTTCC GCAACGAGGA GCTCTACGTG AACCCGTACT CCCGCAACCC GCGCCGCAAG 
CGCAGTCTGA AGCGGCACGC CCAGGCCCAG CGGTCGCCCT CGACGACCCT GCCACCGATC 
ACGTGCTTCG ACCTCTCCCG CGACTCGCAG CTGATGGCCA TCGCCTCAGG CCGCAGCGTG 
CACCTGATGC GCATCAACAC GCCGGAGTAC CAGTGCAGCC TGGAGGGGCA CGCGGGCGTG 
GTCAACTGCC TGAAGTTCGC GCCCAACGGA GAGTTCCTCA CCACGGGATC CGACGATCGG 
CTCGTCCAGG TGTGGAACCT GGCGGCCACC GAGACCATTA ACACCTTCAA GGGGCATGCG 
GCGCCGGTGA TCAAGGTGGT GGTGCTGATG GACTCGCTGC GGGTGATCTC CACGGACCGG 
GACTCACTGC TGCTCGTGTG GATGGCCAGC TCGGGCAACC TGCTGCAGAC CATCCAGGGA 
CCCTACAAGA GCCTGGTGGT GACCAACAAC ATGCGCTTCG CCGCCTCCAG CAACGGGGAC 
AACACTCTGA AGATCTGGTC CCTCACCCAG GAGGACGAGA AGTACTCGGT CTCGCACTCG 
GACGAGATCA CCTGCTTCGA GATCAGCGCC GACAGCATGC ACATCATCAC GGGCTCCCGG 
GACATGTCGC TGAAGGTGTG GCAGTCCACG GGCGGCAAGC TCAGCCAGGT GCTGGTCGGG 
CACAGCGACG CTGTGACCTG TGTGGCCGTC TCGGTGACCA ACAAGACGCA GGTCCTCTCC 
GGCTCCAAGG ACACGAACCT CATCCTGTGG GACCTGCTCA CTGGCGAGGA GGTTCACACG 
CTGGCCGGAC ACTTGGGTCC AGTGATCGGA GTGAAGGTTT CGGCAGATGG CTCCACGGCT 
GTGTCGGGCA GCGATGACAA GACCTTGATC GTGTGGGAGA CGAAGCGCGG ATTGGCCCTG 
ACCTCGCTGC AGATGCACGT GCCCTTCACC CACTTCGACA TCAGCCTGGA GGTGTCGCGG 
GTCCTGGTCC AACTGGTGGA CAGCTACAAC CTCCCGGTGA TCTGTCTGCA CAACACCCCG 
GCGCAGTATG TAAAGCTGCC CACGTACTCG GGACCCACCA AGGATGTGGA TGATCTCCGG 
CCACAGGGCC CGAAGCGCCA GATGAAGCGC CTGCTGAAGA AGGAGGTCTC CCTGGACACG 
TACACTTGGC AGAAGAAGTA CGGCCACCTG ACCTCGTCGG TCATGATGGC CCAGGTTGAC 
GAGCGCCTGA AGCGACGCTT CAGCGTCTCG GCCAGCATGG AGGAGATCTC GAAGATCGCC 
GAGAACAAGA CAGGGGCCTC CAATGCCAAC ATCGGCCCCG AGCAGGCCGC TCTGGCCCAG 
TCGCAGCACT TCGACCAGCT GGAGGCGCTG TGGAACAAGC GGTCTCCGCC CCGACGCCGC 
CACAACGCGG GTCTGTCGAG GCAGACATCG CTGGTGGAGG ACCGGCTCGA GTCGTCGGAC 
GACGACGAGT ACCAGGACGA GCGCGCAGAT CACTTCGCTT CAGGAGGAGG AGGATACCAC 
AAGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA15661 ( XM_001361286.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001361323.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA15661), mRNA.

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CloneID	ODe03971
Clone ID Related Accession (Same CDS sequence)	XM_001361286.3
Accession Version	XM_001361286.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001361286.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC CCATTGACGA GAGTGTGCTG AACGCACTGA AGGGGGTGAC CTCCAAGCAG 
ACCCGCCTGC CCAAGCCGCT GCTCATCAAG ATCTATGTCG CCAGCCAGAA GCAGGAGTTC 
AACCAGGAGC GCCGCATGCT CCTGGAGCTG GTGGGCCCCG AGCTGCAGGG CCTCTACGAC 
GACCGCCAAA TCGAGTTGGA GTTCGTGGAC ATGCACTTCG GCACTGGGGC GCTGGAGGTG 
AACCAGCTGG AGCGGGATCC CTACCTGCTG CAGGACTACC TGCACGAGAT CGACACCTGT 
CATGCCCACA CGAAGAGCGT CTTCTTTCTG GCGCTCATCG GCGACGGCCT CGGACGCCTG 
CCGCTGCCCA CGCGCCTGGA CGAGGACATC TATGCCGCGG TGCTGGCCGA CCCTCAGACA 
ACGGCCGACC AGGAGGCTCT TCTAGCCAGG TGGTACGAGA TGGATCCATC CAAGGTGCAG 
CGTCAGCTGA AGCAGGACTA TCGAGTACTG CCCATGGACG AGTGGCTCAG CGAGTCCCAG 
CAGATGCAAA AGATGCTGGA GCAGGCGCTG CAAGGCTTTG TCCAAGGACC AGGTGCCTCG 
GCGCGATCTG CAGACTTCCT GAAGCGGGTG CAGCAGCTGA GGCGCACGCA GATCGAACGC 
GAAGTGGAGC AGGCCATGGC CCTCTCCAGC GAGAAGATCC TCGCCGTGTT TCGGGAACGG 
GCCGCCCAGT GCAGCAAGCG GGATGTGGAG GCCAATGAGC GGCTTCGGAA GATCAAGGAC 
GAGCTCACCA TGAACCTCTC CACGGACAAT CACACAACCC TAGTGGTGCC AGGCAGTGCC 
TCGTGCGAAA TGATCGACCC GGACAACGAG GACCACGAGT CCTATCTCAG CAAATTCAAG 
AACAAAGTCA CCGACAAGCT GCGTCTGCTA ATCGAGGCGC ACATCACCAA TGATCCCGAT 
GTAATCAAGG GGAGGAAAAA GACTGTTCAG GAAATCTTTC ACGAGCACGC CACTCACCTG 
CGCATCCTGC GCGATCATGT GGAGGGGGCG GCCCTCATCG AGTCGCGGGT GCCACCGCAG 
CTGCGCCAGA ACCTGATGGC GAACTTCAGG AACGGCAGCC GGCATGCGCC GTACTTCATT 
TGCGGCTCGG ATGGCAGCGG CAAGAGCGCC CTGATCAGCA CCCTGTACGG GCAGGTGGCC 
AGCTGGTTCG TCTCGACGAA GGTCCACCGC GTGATCCGCT TCGCGAAGGC CACGCCGCGC 
TCGGCCTACA ACCTGGAGCT GCTGCGCGTG ATCTGCCAGC AGATATCCAT CATCTTCAAC 
ATCCCCGAGG GCTACCTACC CAAGGACGCC TCCTTCGATC CGCTGTACAT CAGCACCTGG 
TTCCAGAACC TGCTGCGGCG CGTCGAGGAC ATGAGCAACG ACGTGCTGTT CCTGTTCATC 
GACGACCTGC ACCTGCTGAA CCCCCTGGAC TGCGACATCG TGACCGCCCT CTCCTGGCTG 
CCCACCTCGC TGCCCTGGAA CGTGCAGATC ATCTGCAGCT CCACCACCCC CGTCGAGCAG 
CTCAAGTTCA CGCCCATGCA GCGGGACCGC TTCAAGTCGG CGGAGTACCA GTTCGACCTC 
AACCTGGGCG AGTACGCCAC GGAGCTGCGG CGACCTCCGC AGTGCGTCTC GGACGTCAGC 
TTCGTCCTGT ACGTGGAGCA GCAGTTCGAC GGCCTGGAGC GCCACTACGG CAGGCAGGCG 
GTGGGCAGCC TGGCCGCCTA CATCACCTGC TCCGAGTACG GGCTGAGCGA GACGGAGCTC 
CTGGAGCTGC TCATGCCCAC CGACGATCCC GAGTCCCTGA TCGACACCAG AAGCGGACAC 
TTTAGCTTCG CCACCTTCAA GAAGATCCAC AGGGAAATGG ACAAGCTCCT GCTGCTGCAC 
GACAAGATCA TGTCCGGCAA GGTGCTCATC CAGTGGCGCC ACAACTACTG CGCCGTGGTG 
GCCAAGCGAC GGTACATGGG CGGCCAGAGG ACGCGCAACC TGCACATGGA GCTGGCCAAC 
CTGTTCTTCC CGCAGGACGA GGACGAGAGC ACGCTGGAGA ACGAGTCCAC CCAGAGCGAG 
AACAAGTCCG TGATTAGCCT CAAGGACACG CTCAGGACAG TCTGCGACCT GCCGCACAAG 
GACAGGGAGC GCGAGAAGGA CAACGTCTCC GCTGGCCGCA AGTCCTCCTC CACCCACCAC 
AACGACGACA CCTCCACCTT CTACAACCCC ATCGCCGCCG ACGTCAGCTA CAGCATGCGG 
CACGTGGAGG AGAGCTGGCA CCACCTGATG CGCTCCGACG ACACGACACG CTTCAAGCAA 
ATCGCAGTCT GCAACTTTGA TTTCCTGCTG GCGGCGGTAC AAACTGTGTC GATCAGCTAT 
TTGCGCTGCC TGATCGAGCA CGTCCGCTGC TATATCCTCG ACAGGGACAT CGAGCTGATC 
TACTACACGA TCCGCAAGTC CAGCGACGTG CTCACGCGCG ACCCCATGCA GCTGGGCTCC 
CAGCTGATCT CCTGGCTGCG TCCGATCAGC GAGCACGACG ACGACGACAA CTCGCTGCTC 
AGCATGACGG TGCGCTCGGC GACGGCCTGG TGCGATGGCT ACGCCGTGCC GCTGCTGGTG 
CCGCTCACGG GATGGCTGCC GGCACCGCTG CCCTCGCAGA TCCGCACCAT GACGGTGTCG 
GGCACGGGCG TCATCCGAGC CGTCTGCCTG GCGCCCTCGA GGCAGCACCT CATCCTGGCC 
ACCAGCTCGG GCGAGGTGCA GCAGTGGCAC ATCATGAGCA ACTCGCTGGA ACACATCTTC 
AAGGGACACA CTGCGGCAGT CACGTGCCTG CTGGTGGCTC CGACCAGCGA GCCGGAGCTC 
CTCCTCACCG GCTCCGAGGA CACCAGCGTG CTCGTCTGGC ACGTCTCGCA GCGCGACCGT 
CGGGCGCACA TCAACGCCCA CACAGCCCCC ATAACGGGCG TGGCGGCCGG GGTCAATAAC 
ACGCTGATCA TCAGCAGCAG CGAGGATGCC ACAATCGCCA TCACGGACCT GGCCAGCGGC 
AAACTGAAAC ACCGAATCAG CCACCATCGC GGCCCTGTGA CCGGGATCCT GGTGGCCGGA 
GCGTGCGATG TCCTGATCTC GGGCAGCCAC GACCGAACCA TCTGCGTGTG GAACCTCGAG 
AACTTTGCGC TGCTGAACAC CATGCAAATG CTGAGCCCCG TCCTGCGCAT CGACATCTCA 
TGGAACTCGG TATTCCTCCT GGCCCTGTGC GAGGACAATG CCCTCTATGT GCGCACTCTG 
GCCACGGGCA AGGAGCTGCA CACGCTCAAG GGCCACAAGT CAAAGATACG GACCATTTCC 
ATTGGCAAGG ACAGTCAGCG CTGCGTGGTG GGATGCGACG ATACGCGAGC CCTGATCTAC 
GACATGCACT CCGGCAAGCT GGTGCGCTCC ATGCCGCCCA ACCCGGGGCC GGTGACCGCC 
GTCTATGCGA TGGAGAACGA CGACTTCCTG GTCACGGTGG GCGGCAACAA GATCACGTTC 
TACTCGTTCC GCAACGAGGA GCTCTACGTG AACCCGTACT CCCGCAACCC GCGCCGCAAG 
CGCAGTCTGA AGCGGCACGC CCAGGCCCAG CGGTCGCCCT CGACGACCCT GCCACCGATC 
ACGTGCTTCG ACCTCTCCCG CGACTCGCAG CTGATGGCCA TCGCCTCAGG CCGCAGCGTG 
CACCTGATGC GCATCAACAC GCCGGAGTAC CAGTGCAGCC TGGAGGGGCA CGCGGGCGTG 
GTCAACTGCC TGAAGTTCGC GCCCAACGGA GAGTTCCTCA CCACGGGATC CGACGATCGG 
CTCGTCCAGG TGTGGAACCT GGCGGCCACC GAGACCATTA ACACCTTCAA GGGGCATGCG 
GCGCCGGTGA TCAAGGTGGT GGTGCTGATG GACTCGCTGC GGGTGATCTC CACGGACCGG 
GACTCACTGC TGCTCGTGTG GATGGCCAGC TCGGGCAACC TGCTGCAGAC CATCCAGGGA 
CCCTACAAGA GCCTGGTGGT GACCAACAAC ATGCGCTTCG CCGCCTCCAG CAACGGGGAC 
AACACTCTGA AGATCTGGTC CCTCACCCAG GAGGACGAGA AGTACTCGGT CTCGCACTCG 
GACGAGATCA CCTGCTTCGA GATCAGCGCC GACAGCATGC ACATCATCAC GGGCTCCCGG 
GACATGTCGC TGAAGGTGTG GCAGTCCACG GGCGGCAAGC TCAGCCAGGT GCTGGTCGGG 
CACAGCGACG CTGTGACCTG TGTGGCCGTC TCGGTGACCA ACAAGACGCA GGTCCTCTCC 
GGCTCCAAGG ACACGAACCT CATCCTGTGG GACCTGCTCA CTGGCGAGGA GGTTCACACG 
CTGGCCGGAC ACTTGGGTCC AGTGATCGGA GTGAAGGTTT CGGCAGATGG CTCCACGGCT 
GTGTCGGGCA GCGATGACAA GACCTTGATC GTGTGGGAGA CGAAGCGCGG ATTGGCCCTG 
ACCTCGCTGC AGATGCACGT GCCCTTCACC CACTTCGACA TCAGCCTGGA GGTGTCGCGG 
GTCCTGGTCC AACTGGTGGA CAGCTACAAC CTCCCGGTGA TCTGTCTGCA CAACACCCCG 
GCGCAGTATG TAAAGCTGCC CACGTACTCG GGACCCACCA AGGATGTGGA TGATCTCCGG 
CCACAGGGCC CGAAGCGCCA GATGAAGCGC CTGCTGAAGA AGGAGGTCTC CCTGGACACG 
TACACTTGGC AGAAGAAGTA CGGCCACCTG ACCTCGTCGG TCATGATGGC CCAGGTTGAC 
GAGCGCCTGA AGCGACGCTT CAGCGTCTCG GCCAGCATGG AGGAGATCTC GAAGATCGCC 
GAGAACAAGA CAGGGGCCTC CAATGCCAAC ATCGGCCCCG AGCAGGCCGC TCTGGCCCAG 
TCGCAGCACT TCGACCAGCT GGAGGCGCTG TGGAACAAGC GGTCTCCGCC CCGACGCCGC 
CACAACGCGG GTCTGTCGAG GCAGACATCG CTGGTGGAGG ACCGGCTCGA GTCGTCGGAC 
GACGACGAGT ACCAGGACGA GCGCGCAGCC AACGCCCACA GTTCGAAAAC CAGTTCCCGC 
AGCTCCCTGC ACCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001361323.1
CDS	550..5667
Translation	MAAPIDESVLNALKGVTSKQTRLPKPLLIKIYVASQKQEFNQERRMLLELVGPELQGLYDDRQIELEFVDMHFGTGALEVNQLERDPYLLQDYLHEIDTCHAHTKSVFFLALIGDGLGRLPLPTRLDEDIYAAVLADPQTTADQEALLARWYEMDPSKVQRQLKQDYRVLPMDEWLSESQQMQKMLEQALQGFVQGPGASARSADFLKRVQQLRRTQIEREVEQAMALSSEKILAVFRERAAQCSKRDVEANERLRKIKDELTMNLSTDNHTTLVVPGSASCEMIDPDNEDHESYLSKFKNKVTDKLRLLIEAHITNDPDVIKGRKKTVQEIFHEHATHLRILRDHVEGAALIESRVPPQLRQNLMANFRNGSRHAPYFICGSDGSGKSALISTLYGQVASWFVSTKVHRVIRFAKATPRSAYNLELLRVICQQISIIFNIPEGYLPKDASFDPLYISTWFQNLLRRVEDMSNDVLFLFIDDLHLLNPLDCDIVTALSWLPTSLPWNVQIICSSTTPVEQLKFTPMQRDRFKSAEYQFDLNLGEYATELRRPPQCVSDVSFVLYVEQQFDGLERHYGRQAVGSLAAYITCSEYGLSETELLELLMPTDDPESLIDTRSGHFSFATFKKIHREMDKLLLLHDKIMSGKVLIQWRHNYCAVVAKRRYMGGQRTRNLHMELANLFFPQDEDESTLENESTQSENKSVISLKDTLRTVCDLPHKDREREKDNVSAGRKSSSTHHNDDTSTFYNPIAADVSYSMRHVEESWHHLMRSDDTTRFKQIAVCNFDFLLAAVQTVSISYLRCLIEHVRCYILDRDIELIYYTIRKSSDVLTRDPMQLGSQLISWLRPISEHDDDDNSLLSMTVRSATAWCDGYAVPLLVPLTGWLPAPLPSQIRTMTVSGTGVIRAVCLAPSRQHLILATSSGEVQQWHIMSNSLEHIFKGHTAAVTCLLVAPTSEPELLLTGSEDTSVLVWHVSQRDRRAHINAHTAPITGVAAGVNNTLIISSSEDATIAITDLASGKLKHRISHHRGPVTGILVAGACDVLISGSHDRTICVWNLENFALLNTMQMLSPVLRIDISWNSVFLLALCEDNALYVRTLATGKELHTLKGHKSKIRTISIGKDSQRCVVGCDDTRALIYDMHSGKLVRSMPPNPGPVTAVYAMENDDFLVTVGGNKITFYSFRNEELYVNPYSRNPRRKRSLKRHAQAQRSPSTTLPPITCFDLSRDSQLMAIASGRSVHLMRINTPEYQCSLEGHAGVVNCLKFAPNGEFLTTGSDDRLVQVWNLAATETINTFKGHAAPVIKVVVLMDSLRVISTDRDSLLLVWMASSGNLLQTIQGPYKSLVVTNNMRFAASSNGDNTLKIWSLTQEDEKYSVSHSDEITCFEISADSMHIITGSRDMSLKVWQSTGGKLSQVLVGHSDAVTCVAVSVTNKTQVLSGSKDTNLILWDLLTGEEVHTLAGHLGPVIGVKVSADGSTAVSGSDDKTLIVWETKRGLALTSLQMHVPFTHFDISLEVSRVLVQLVDSYNLPVICLHNTPAQYVKLPTYSGPTKDVDDLRPQGPKRQMKRLLKKEVSLDTYTWQKKYGHLTSSVMMAQVDERLKRRFSVSASMEEISKIAENKTGASNANIGPEQAALAQSQHFDQLEALWNKRSPPRRRHNAGLSRQTSLVEDRLESSDDDEYQDERAANAHSSKTSSRSSLHP

Target ORF information:

RefSeq Version	XM_001361286.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA15661), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001361286.3

ORF Insert Sequence:

ATGGCCGCGC CCATTGACGA GAGTGTGCTG AACGCACTGA AGGGGGTGAC CTCCAAGCAG 
ACCCGCCTGC CCAAGCCGCT GCTCATCAAG ATCTATGTCG CCAGCCAGAA GCAGGAGTTC 
AACCAGGAGC GCCGCATGCT CCTGGAGCTG GTGGGCCCCG AGCTGCAGGG CCTCTACGAC 
GACCGCCAAA TCGAGTTGGA GTTCGTGGAC ATGCACTTCG GCACTGGGGC GCTGGAGGTG 
AACCAGCTGG AGCGGGATCC CTACCTGCTG CAGGACTACC TGCACGAGAT CGACACCTGT 
CATGCCCACA CGAAGAGCGT CTTCTTTCTG GCGCTCATCG GCGACGGCCT CGGACGCCTG 
CCGCTGCCCA CGCGCCTGGA CGAGGACATC TATGCCGCGG TGCTGGCCGA CCCTCAGACA 
ACGGCCGACC AGGAGGCTCT TCTAGCCAGG TGGTACGAGA TGGATCCATC CAAGGTGCAG 
CGTCAGCTGA AGCAGGACTA TCGAGTACTG CCCATGGACG AGTGGCTCAG CGAGTCCCAG 
CAGATGCAAA AGATGCTGGA GCAGGCGCTG CAAGGCTTTG TCCAAGGACC AGGTGCCTCG 
GCGCGATCTG CAGACTTCCT GAAGCGGGTG CAGCAGCTGA GGCGCACGCA GATCGAACGC 
GAAGTGGAGC AGGCCATGGC CCTCTCCAGC GAGAAGATCC TCGCCGTGTT TCGGGAACGG 
GCCGCCCAGT GCAGCAAGCG GGATGTGGAG GCCAATGAGC GGCTTCGGAA GATCAAGGAC 
GAGCTCACCA TGAACCTCTC CACGGACAAT CACACAACCC TAGTGGTGCC AGGCAGTGCC 
TCGTGCGAAA TGATCGACCC GGACAACGAG GACCACGAGT CCTATCTCAG CAAATTCAAG 
AACAAAGTCA CCGACAAGCT GCGTCTGCTA ATCGAGGCGC ACATCACCAA TGATCCCGAT 
GTAATCAAGG GGAGGAAAAA GACTGTTCAG GAAATCTTTC ACGAGCACGC CACTCACCTG 
CGCATCCTGC GCGATCATGT GGAGGGGGCG GCCCTCATCG AGTCGCGGGT GCCACCGCAG 
CTGCGCCAGA ACCTGATGGC GAACTTCAGG AACGGCAGCC GGCATGCGCC GTACTTCATT 
TGCGGCTCGG ATGGCAGCGG CAAGAGCGCC CTGATCAGCA CCCTGTACGG GCAGGTGGCC 
AGCTGGTTCG TCTCGACGAA GGTCCACCGC GTGATCCGCT TCGCGAAGGC CACGCCGCGC 
TCGGCCTACA ACCTGGAGCT GCTGCGCGTG ATCTGCCAGC AGATATCCAT CATCTTCAAC 
ATCCCCGAGG GCTACCTACC CAAGGACGCC TCCTTCGATC CGCTGTACAT CAGCACCTGG 
TTCCAGAACC TGCTGCGGCG CGTCGAGGAC ATGAGCAACG ACGTGCTGTT CCTGTTCATC 
GACGACCTGC ACCTGCTGAA CCCCCTGGAC TGCGACATCG TGACCGCCCT CTCCTGGCTG 
CCCACCTCGC TGCCCTGGAA CGTGCAGATC ATCTGCAGCT CCACCACCCC CGTCGAGCAG 
CTCAAGTTCA CGCCCATGCA GCGGGACCGC TTCAAGTCGG CGGAGTACCA GTTCGACCTC 
AACCTGGGCG AGTACGCCAC GGAGCTGCGG CGACCTCCGC AGTGCGTCTC GGACGTCAGC 
TTCGTCCTGT ACGTGGAGCA GCAGTTCGAC GGCCTGGAGC GCCACTACGG CAGGCAGGCG 
GTGGGCAGCC TGGCCGCCTA CATCACCTGC TCCGAGTACG GGCTGAGCGA GACGGAGCTC 
CTGGAGCTGC TCATGCCCAC CGACGATCCC GAGTCCCTGA TCGACACCAG AAGCGGACAC 
TTTAGCTTCG CCACCTTCAA GAAGATCCAC AGGGAAATGG ACAAGCTCCT GCTGCTGCAC 
GACAAGATCA TGTCCGGCAA GGTGCTCATC CAGTGGCGCC ACAACTACTG CGCCGTGGTG 
GCCAAGCGAC GGTACATGGG CGGCCAGAGG ACGCGCAACC TGCACATGGA GCTGGCCAAC 
CTGTTCTTCC CGCAGGACGA GGACGAGAGC ACGCTGGAGA ACGAGTCCAC CCAGAGCGAG 
AACAAGTCCG TGATTAGCCT CAAGGACACG CTCAGGACAG TCTGCGACCT GCCGCACAAG 
GACAGGGAGC GCGAGAAGGA CAACGTCTCC GCTGGCCGCA AGTCCTCCTC CACCCACCAC 
AACGACGACA CCTCCACCTT CTACAACCCC ATCGCCGCCG ACGTCAGCTA CAGCATGCGG 
CACGTGGAGG AGAGCTGGCA CCACCTGATG CGCTCCGACG ACACGACACG CTTCAAGCAA 
ATCGCAGTCT GCAACTTTGA TTTCCTGCTG GCGGCGGTAC AAACTGTGTC GATCAGCTAT 
TTGCGCTGCC TGATCGAGCA CGTCCGCTGC TATATCCTCG ACAGGGACAT CGAGCTGATC 
TACTACACGA TCCGCAAGTC CAGCGACGTG CTCACGCGCG ACCCCATGCA GCTGGGCTCC 
CAGCTGATCT CCTGGCTGCG TCCGATCAGC GAGCACGACG ACGACGACAA CTCGCTGCTC 
AGCATGACGG TGCGCTCGGC GACGGCCTGG TGCGATGGCT ACGCCGTGCC GCTGCTGGTG 
CCGCTCACGG GATGGCTGCC GGCACCGCTG CCCTCGCAGA TCCGCACCAT GACGGTGTCG 
GGCACGGGCG TCATCCGAGC CGTCTGCCTG GCGCCCTCGA GGCAGCACCT CATCCTGGCC 
ACCAGCTCGG GCGAGGTGCA GCAGTGGCAC ATCATGAGCA ACTCGCTGGA ACACATCTTC 
AAGGGACACA CTGCGGCAGT CACGTGCCTG CTGGTGGCTC CGACCAGCGA GCCGGAGCTC 
CTCCTCACCG GCTCCGAGGA CACCAGCGTG CTCGTCTGGC ACGTCTCGCA GCGCGACCGT 
CGGGCGCACA TCAACGCCCA CACAGCCCCC ATAACGGGCG TGGCGGCCGG GGTCAATAAC 
ACGCTGATCA TCAGCAGCAG CGAGGATGCC ACAATCGCCA TCACGGACCT GGCCAGCGGC 
AAACTGAAAC ACCGAATCAG CCACCATCGC GGCCCTGTGA CCGGGATCCT GGTGGCCGGA 
GCGTGCGATG TCCTGATCTC GGGCAGCCAC GACCGAACCA TCTGCGTGTG GAACCTCGAG 
AACTTTGCGC TGCTGAACAC CATGCAAATG CTGAGCCCCG TCCTGCGCAT CGACATCTCA 
TGGAACTCGG TATTCCTCCT GGCCCTGTGC GAGGACAATG CCCTCTATGT GCGCACTCTG 
GCCACGGGCA AGGAGCTGCA CACGCTCAAG GGCCACAAGT CAAAGATACG GACCATTTCC 
ATTGGCAAGG ACAGTCAGCG CTGCGTGGTG GGATGCGACG ATACGCGAGC CCTGATCTAC 
GACATGCACT CCGGCAAGCT GGTGCGCTCC ATGCCGCCCA ACCCGGGGCC GGTGACCGCC 
GTCTATGCGA TGGAGAACGA CGACTTCCTG GTCACGGTGG GCGGCAACAA GATCACGTTC 
TACTCGTTCC GCAACGAGGA GCTCTACGTG AACCCGTACT CCCGCAACCC GCGCCGCAAG 
CGCAGTCTGA AGCGGCACGC CCAGGCCCAG CGGTCGCCCT CGACGACCCT GCCACCGATC 
ACGTGCTTCG ACCTCTCCCG CGACTCGCAG CTGATGGCCA TCGCCTCAGG CCGCAGCGTG 
CACCTGATGC GCATCAACAC GCCGGAGTAC CAGTGCAGCC TGGAGGGGCA CGCGGGCGTG 
GTCAACTGCC TGAAGTTCGC GCCCAACGGA GAGTTCCTCA CCACGGGATC CGACGATCGG 
CTCGTCCAGG TGTGGAACCT GGCGGCCACC GAGACCATTA ACACCTTCAA GGGGCATGCG 
GCGCCGGTGA TCAAGGTGGT GGTGCTGATG GACTCGCTGC GGGTGATCTC CACGGACCGG 
GACTCACTGC TGCTCGTGTG GATGGCCAGC TCGGGCAACC TGCTGCAGAC CATCCAGGGA 
CCCTACAAGA GCCTGGTGGT GACCAACAAC ATGCGCTTCG CCGCCTCCAG CAACGGGGAC 
AACACTCTGA AGATCTGGTC CCTCACCCAG GAGGACGAGA AGTACTCGGT CTCGCACTCG 
GACGAGATCA CCTGCTTCGA GATCAGCGCC GACAGCATGC ACATCATCAC GGGCTCCCGG 
GACATGTCGC TGAAGGTGTG GCAGTCCACG GGCGGCAAGC TCAGCCAGGT GCTGGTCGGG 
CACAGCGACG CTGTGACCTG TGTGGCCGTC TCGGTGACCA ACAAGACGCA GGTCCTCTCC 
GGCTCCAAGG ACACGAACCT CATCCTGTGG GACCTGCTCA CTGGCGAGGA GGTTCACACG 
CTGGCCGGAC ACTTGGGTCC AGTGATCGGA GTGAAGGTTT CGGCAGATGG CTCCACGGCT 
GTGTCGGGCA GCGATGACAA GACCTTGATC GTGTGGGAGA CGAAGCGCGG ATTGGCCCTG 
ACCTCGCTGC AGATGCACGT GCCCTTCACC CACTTCGACA TCAGCCTGGA GGTGTCGCGG 
GTCCTGGTCC AACTGGTGGA CAGCTACAAC CTCCCGGTGA TCTGTCTGCA CAACACCCCG 
GCGCAGTATG TAAAGCTGCC CACGTACTCG GGACCCACCA AGGATGTGGA TGATCTCCGG 
CCACAGGGCC CGAAGCGCCA GATGAAGCGC CTGCTGAAGA AGGAGGTCTC CCTGGACACG 
TACACTTGGC AGAAGAAGTA CGGCCACCTG ACCTCGTCGG TCATGATGGC CCAGGTTGAC 
GAGCGCCTGA AGCGACGCTT CAGCGTCTCG GCCAGCATGG AGGAGATCTC GAAGATCGCC 
GAGAACAAGA CAGGGGCCTC CAATGCCAAC ATCGGCCCCG AGCAGGCCGC TCTGGCCCAG 
TCGCAGCACT TCGACCAGCT GGAGGCGCTG TGGAACAAGC GGTCTCCGCC CCGACGCCGC 
CACAACGCGG GTCTGTCGAG GCAGACATCG CTGGTGGAGG ACCGGCTCGA GTCGTCGGAC 
GACGACGAGT ACCAGGACGA GCGCGCAGCC AACGCCCACA GTTCGAAAAC CAGTTCCCGC 
AGCTCCCTGC ACCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA15661 ( XM_015184738.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015040224.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA15661), mRNA.

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CloneID	ODe03972
Clone ID Related Accession (Same CDS sequence)	XM_015184738.1 , XM_004444251.2
Accession Version	XM_015184738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC CCATTGACGA GAGTGTGCTG AACGCACTGA AGGGGGTGAC CTCCAAGCAG 
ACCCGCCTGC CCAAGCCGCT GCTCATCAAG ATCTATGTCG CCAGCCAGAA GCAGGAGTTC 
AACCAGGAGC GCCGCATGCT CCTGGAGCTG GTGGGCCCCG AGCTGCAGGG CCTCTACGAC 
GACCGCCAAA TCGAGTTGGA GTTCGTGGAC ATGCACTTCG GCACTGGGGC GCTGGAGGTG 
AACCAGCTGG AGCGGGATCC CTACCTGCTG CAGGACTACC TGCACGAGAT CGACACCTGT 
CATGCCCACA CGAAGAGCGT CTTCTTTCTG GCGCTCATCG GCGACGGCCT CGGACGCCTG 
CCGCTGCCCA CGCGCCTGGA CGAGGACATC TATGCCGCGG TGCTGGCCGA CCCTCAGACA 
ACGGCCGACC AGGAGGCTCT TCTAGCCAGG TGGTACGAGA TGGATCCATC CAAGGTGCAG 
CGTCAGCTGA AGCAGGACTA TCGAGTACTG CCCATGGACG AGTGGCTCAG CGAGTCCCAG 
CAGATGCAAA AGATGCTGGA GCAGGCGCTG CAAGGCTTTG TCCAAGGACC AGGTGCCTCG 
GCGCGATCTG CAGACTTCCT GAAGCGGGTG CAGCAGCTGA GGCGCACGCA GATCGAACGC 
GAAGTGGAGC AGGCCATGGC CCTCTCCAGC GAGAAGATCC TCGCCGTGTT TCGGGAACGG 
GCCGCCCAGT GCAGCAAGCG GGATGTGGAG GCCAATGAGC GGCTTCGGAA GATCAAGGAC 
GAGCTCACCA TGAACCTCTC CACGGACAAT CACACAACCC TAGTGGTGCC AGGCAGTGCC 
TCGTGCGAAA TGATCGACCC GGACAACGAG GACCACGAGT CCTATCTCAG CAAATTCAAG 
AACAAAGTCA CCGACAAGCT GCGTCTGCTA ATCGAGGCGC ACATCACCAA TGATCCCGAT 
GTAATCAAGG GGAGGAAAAA GACTGTTCAG GAAATCTTTC ACGAGCACGC CACTCACCTG 
CGCATCCTGC GCGATCATGT GGAGGGGGCG GCCCTCATCG AGTCGCGGGT GCCACCGCAG 
CTGCGCCAGA ACCTGATGGC GAACTTCAGG AACGGCAGCC GGCATGCGCC GTACTTCATT 
TGCGGCTCGG ATGGCAGCGG CAAGAGCGCC CTGATCAGCA CCCTGTACGG GCAGGTGGCC 
AGCTGGTTCG TCTCGACGAA GGTCCACCGC GTGATCCGCT TCGCGAAGGC CACGCCGCGC 
TCGGCCTACA ACCTGGAGCT GCTGCGCGTG ATCTGCCAGC AGATATCCAT CATCTTCAAC 
ATCCCCGAGG GCTACCTACC CAAGGACGCC TCCTTCGATC CGCTGTACAT CAGCACCTGG 
TTCCAGAACC TGCTGCGGCG CGTCGAGGAC ATGAGCAACG ACGTGCTGTT CCTGTTCATC 
GACGACCTGC ACCTGCTGAA CCCCCTGGAC TGCGACATCG TGACCGCCCT CTCCTGGCTG 
CCCACCTCGC TGCCCTGGAA CGTGCAGATC ATCTGCAGCT CCACCACCCC CGTCGAGCAG 
CTCAAGTTCA CGCCCATGCA GCGGGACCGC TTCAAGTCGG CGGAGTACCA GTTCGACCTC 
AACCTGGGCG AGTACGCCAC GGAGCTGCGG CGACCTCCGC AGTGCGTCTC GGACGTCAGC 
TTCGTCCTGT ACGTGGAGCA GCAGTTCGAC GGCCTGGAGC GCCACTACGG CAGGCAGGCG 
GTGGGCAGCC TGGCCGCCTA CATCACCTGC TCCGAGTACG GGCTGAGCGA GACGGAGCTC 
CTGGAGCTGC TCATGCCCAC CGACGATCCC GAGTCCCTGA TCGACACCAG AAGCGGACAC 
TTTAGCTTCG CCACCTTCAA GAAGATCCAC AGGGAAATGG ACAAGCTCCT GCTGCTGCAC 
GACAAGATCA TGTCCGGCAA GGTGCTCATC CAGTGGCGCC ACAACTACTG CGCCGTGGTG 
GCCAAGCGAC GGTACATGGG CGGCCAGAGG ACGCGCAACC TGCACATGGA GCTGGCCAAC 
CTGTTCTTCC CGCAGGACGA GGACGAGAGC ACGCTGGAGA ACGAGTCCAC CCAGAGCGAG 
AACAAGTCCG TGATTAGCCT CAAGGACACG CTCAGGACAG TCTGCGACCT GCCGCACAAG 
GACAGGGAGC GCGAGAAGGA CAACGTCTCC GCTGGCCGCA AGTCCTCCTC CACCCACCAC 
AACGACGACA CCTCCACCTT CTACAACCCC ATCGCCGCCG ACGTCAGCTA CAGCATGCGG 
CACGTGGAGG AGAGCTGGCA CCACCTGATG CGCTCCGACG ACACGACACG CTTCAAGCAA 
ATCGCAGTCT GCAACTTTGA TTTCCTGCTG GCGGCGGTAC AAACTGTGTC GATCAGCTAT 
TTGCGCTGCC TGATCGAGCA CGTCCGCTGC TATATCCTCG ACAGGGACAT CGAGCTGATC 
TACTACACGA TCCGCAAGTC CAGCGACGTG CTCACGCGCG ACCCCATGCA GCTGGGCTCC 
CAGCTGATCT CCTGGCTGCG TCCGATCAGC GAGCACGACG ACGACGACAA CTCGCTGCTC 
AGCATGACGG TGCGCTCGGC GACGGCCTGG TGCGATGGCT ACGCCGTGCC GCTGCTGGTG 
CCGCTCACGG GATGGCTGCC GGCACCGCTG CCCTCGCAGA TCCGCACCAT GACGGTGTCG 
GGCACGGGCG TCATCCGAGC CGTCTGCCTG GCGCCCTCGA GGCAGCACCT CATCCTGGCC 
ACCAGCTCGG GCGAGGTGCA GCAGTGGCAC ATCATGAGCA ACTCGCTGGA ACACATCTTC 
AAGGGACACA CTGCGGCAGT CACGTGCCTG CTGGTGGCTC CGACCAGCGA GCCGGAGCTC 
CTCCTCACCG GCTCCGAGGA CACCAGCGTG CTCGTCTGGC ACGTCTCGCA GCGCGACCGT 
CGGGCGCACA TCAACGCCCA CACAGCCCCC ATAACGGGCG TGGCGGCCGG GGTCAATAAC 
ACGCTGATCA TCAGCAGCAG CGAGGATGCC ACAATCGCCA TCACGGACCT GGCCAGCGGC 
AAACTGAAAC ACCGAATCAG CCACCATCGC GGCCCTGTGA CCGGGATCCT GGTGGCCGGA 
GCGTGCGATG TCCTGATCTC GGGCAGCCAC GACCGAACCA TCTGCGTGTG GAACCTCGAG 
AACTTTGCGC TGCTGAACAC CATGCAAATG CTGAGCCCCG TCCTGCGCAT CGACATCTCA 
TGGAACTCGG TATTCCTCCT GGCCCTGTGC GAGGACAATG CCCTCTATGT GCGCACTCTG 
GCCACGGGCA AGGAGCTGCA CACGCTCAAG GGCCACAAGT CAAAGATACG GACCATTTCC 
ATTGGCAAGG ACAGTCAGCG CTGCGTGGTG GGATGCGACG ATACGCGAGC CCTGATCTAC 
GACATGCACT CCGGCAAGCT GGTGCGCTCC ATGCCGCCCA ACCCGGGGCC GGTGACCGCC 
GTCTATGCGA TGGAGAACGA CGACTTCCTG GTCACGGTGG GCGGCAACAA GATCACGTTC 
TACTCGTTCC GCAACGAGGA GCTCTACGTG AACCCGTACT CCCGCAACCC GCGCCGCAAG 
CGCAGTCTGA AGCGGCACGC CCAGGCCCAG CGGTCGCCCT CGACGACCCT GCCACCGATC 
ACGTGCTTCG ACCTCTCCCG CGACTCGCAG CTGATGGCCA TCGCCTCAGG CCGCAGCGTG 
CACCTGATGC GCATCAACAC GCCGGAGTAC CAGTGCAGCC TGGAGGGGCA CGCGGGCGTG 
GTCAACTGCC TGAAGTTCGC GCCCAACGGA GAGTTCCTCA CCACGGGATC CGACGATCGG 
CTCGTCCAGG TGTGGAACCT GGCGGCCACC GAGACCATTA ACACCTTCAA GGGGCATGCG 
GCGCCGGTGA TCAAGGTGGT GGTGCTGATG GACTCGCTGC GGGTGATCTC CACGGACCGG 
GACTCACTGC TGCTCGTGTG GATGGCCAGC TCGGGCAACC TGCTGCAGAC CATCCAGGGA 
CCCTACAAGA GCCTGGTGGT GACCAACAAC ATGCGCTTCG CCGCCTCCAG CAACGGGGAC 
AACACTCTGA AGATCTGGTC CCTCACCCAG GAGGACGAGA AGTACTCGGT CTCGCACTCG 
GACGAGATCA CCTGCTTCGA GATCAGCGCC GACAGCATGC ACATCATCAC GGGCTCCCGG 
GACATGTCGC TGAAGGTGTG GCAGTCCACG GGCGGCAAGC TCAGCCAGGT GCTGGTCGGG 
CACAGCGACG CTGTGACCTG TGTGGCCGTC TCGGTGACCA ACAAGACGCA GGTCCTCTCC 
GGCTCCAAGG ACACGAACCT CATCCTGTGG GACCTGCTCA CTGGCGAGGA GGTTCACACG 
CTGGCCGGAC ACTTGGGTCC AGTGATCGGA GTGAAGGTTT CGGCAGATGG CTCCACGGCT 
GTGTCGGGCA GCGATGACAA GACCTTGATC GTGTGGGAGA CGAAGCGCGG ATTGGCCCTG 
ACCTCGCTGC AGATGCACGT GCCCTTCACC CACTTCGACA TCAGCCTGGA GGTGTCGCGG 
GTCCTGGTCC AACTGGTGGA CAGCTACAAC CTCCCGGTGA TCTGTCTGCA CAACACCCCG 
GCGCAGTATG TAAAGCTGCC CACGTACTCG GGACCCACCA AGGATGTGGA TGATCTCCGG 
CCACAGGGCC CGAAGCGCCA GATGAAGCGC CTGCTGAAGA AGGAGGTCTC CCTGGACACG 
TACACTTGGC AGAAGAAGTA CGGCCACCTG ACCTCGTCGG TCATGATGGC CCAGGTTGAC 
GAGCGCCTGA AGCGACGCTT CAGCGTCTCG GCCAGCATGG AGGAGATCTC GAAGATCGCC 
GAGAACAAGA CAGGGGCCTC CAATGCCAAC ATCGGCCCCG AGCAGGCCGC TCTGGCCCAG 
TCGCAGCACT TCGACCAGCT GGAGGCGCTG TGGAACAAGC GGTCTCCGCC CCGACGCCGC 
CACAACGCGG GTCTGTCGAG GCAGACATCG CTGGTGGAGG ACCGGCTCGA GTCGTCGGAC 
GACGACGAGT ACCAGGACGA GCGCGCAGGT ATGTTGTCAG CCTCCTACGA CCACATCCAC 
CTCCTTGCAC GCTTGCCTGG CATCCGCTCC CTGAACGATG CATGGAAGAC AAACAGTCTG 
CGAGTGCCGA GAACCAAGTT CTACGTGAGC ACCCCCCCAC AGACCAACAG CAGCCACCAG 
CTGAACGCGA CCAGTCGGAC AGAACCCCCC TCCTCCGAAC AGAACGCCGA CAGCAGTGCG 
ACCCTCCACC GGGTGGCGCC CGACTTGGTG AGGGATCTGC TACACCCGGT CCCCTCACCG 
GCAGAGTCCC CTCGGCTGAC CACTCTGCGC CAGTTCTTCC ACTGGCCCAG CCTGAAGTCG 
CACCTGATGC GCGGTCAGCG CCAGAGGAAC TGCCTGTCCG TCCAGCCACA CATGCGCCTG 
AATCCGCCGG ACCCCCTCCG ACCCACGCCC ATCATCGTGG TGGAGAACTG TGACAGTCGC 
AATGTGCAGC GCCTGCGCAC CGATCTTGTC CTGCATCCGC AGTACAGTCG CGCCTCATTC 
GGCCACATTG AGGCGGACGA GCACGAGGTT CCCTTGGCCA AGAGGTCATC TTCCTCGAGT 
GGCGGCTACC TCCACCGCCG CTGGCACCTC CTGCGCCACA AATCCCCTTC CGAACGGAGC 
GGTCCCAAAG GCAAAGCCGA AGCCAATTCC AATACAAACT CGAGCTCCCA AGAGTCAACA 
GCCACTTGCA ACGAGTGTGT GGCTCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015040224.1
CDS	509..6298
Translation	MAAPIDESVLNALKGVTSKQTRLPKPLLIKIYVASQKQEFNQERRMLLELVGPELQGLYDDRQIELEFVDMHFGTGALEVNQLERDPYLLQDYLHEIDTCHAHTKSVFFLALIGDGLGRLPLPTRLDEDIYAAVLADPQTTADQEALLARWYEMDPSKVQRQLKQDYRVLPMDEWLSESQQMQKMLEQALQGFVQGPGASARSADFLKRVQQLRRTQIEREVEQAMALSSEKILAVFRERAAQCSKRDVEANERLRKIKDELTMNLSTDNHTTLVVPGSASCEMIDPDNEDHESYLSKFKNKVTDKLRLLIEAHITNDPDVIKGRKKTVQEIFHEHATHLRILRDHVEGAALIESRVPPQLRQNLMANFRNGSRHAPYFICGSDGSGKSALISTLYGQVASWFVSTKVHRVIRFAKATPRSAYNLELLRVICQQISIIFNIPEGYLPKDASFDPLYISTWFQNLLRRVEDMSNDVLFLFIDDLHLLNPLDCDIVTALSWLPTSLPWNVQIICSSTTPVEQLKFTPMQRDRFKSAEYQFDLNLGEYATELRRPPQCVSDVSFVLYVEQQFDGLERHYGRQAVGSLAAYITCSEYGLSETELLELLMPTDDPESLIDTRSGHFSFATFKKIHREMDKLLLLHDKIMSGKVLIQWRHNYCAVVAKRRYMGGQRTRNLHMELANLFFPQDEDESTLENESTQSENKSVISLKDTLRTVCDLPHKDREREKDNVSAGRKSSSTHHNDDTSTFYNPIAADVSYSMRHVEESWHHLMRSDDTTRFKQIAVCNFDFLLAAVQTVSISYLRCLIEHVRCYILDRDIELIYYTIRKSSDVLTRDPMQLGSQLISWLRPISEHDDDDNSLLSMTVRSATAWCDGYAVPLLVPLTGWLPAPLPSQIRTMTVSGTGVIRAVCLAPSRQHLILATSSGEVQQWHIMSNSLEHIFKGHTAAVTCLLVAPTSEPELLLTGSEDTSVLVWHVSQRDRRAHINAHTAPITGVAAGVNNTLIISSSEDATIAITDLASGKLKHRISHHRGPVTGILVAGACDVLISGSHDRTICVWNLENFALLNTMQMLSPVLRIDISWNSVFLLALCEDNALYVRTLATGKELHTLKGHKSKIRTISIGKDSQRCVVGCDDTRALIYDMHSGKLVRSMPPNPGPVTAVYAMENDDFLVTVGGNKITFYSFRNEELYVNPYSRNPRRKRSLKRHAQAQRSPSTTLPPITCFDLSRDSQLMAIASGRSVHLMRINTPEYQCSLEGHAGVVNCLKFAPNGEFLTTGSDDRLVQVWNLAATETINTFKGHAAPVIKVVVLMDSLRVISTDRDSLLLVWMASSGNLLQTIQGPYKSLVVTNNMRFAASSNGDNTLKIWSLTQEDEKYSVSHSDEITCFEISADSMHIITGSRDMSLKVWQSTGGKLSQVLVGHSDAVTCVAVSVTNKTQVLSGSKDTNLILWDLLTGEEVHTLAGHLGPVIGVKVSADGSTAVSGSDDKTLIVWETKRGLALTSLQMHVPFTHFDISLEVSRVLVQLVDSYNLPVICLHNTPAQYVKLPTYSGPTKDVDDLRPQGPKRQMKRLLKKEVSLDTYTWQKKYGHLTSSVMMAQVDERLKRRFSVSASMEEISKIAENKTGASNANIGPEQAALAQSQHFDQLEALWNKRSPPRRRHNAGLSRQTSLVEDRLESSDDDEYQDERAGMLSASYDHIHLLARLPGIRSLNDAWKTNSLRVPRTKFYVSTPPQTNSSHQLNATSRTEPPSSEQNADSSATLHRVAPDLVRDLLHPVPSPAESPRLTTLRQFFHWPSLKSHLMRGQRQRNCLSVQPHMRLNPPDPLRPTPIIVVENCDSRNVQRLRTDLVLHPQYSRASFGHIEADEHEVPLAKRSSSSSGGYLHRRWHLLRHKSPSERSGPKGKAEANSNTNSSSQESTATCNECVAQ

Target ORF information:

RefSeq Version	XM_015184738.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA15661), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015184738.1

ORF Insert Sequence:

ATGGCCGCGC CCATTGACGA GAGTGTGCTG AACGCACTGA AGGGGGTGAC CTCCAAGCAG 
ACCCGCCTGC CCAAGCCGCT GCTCATCAAG ATCTATGTCG CCAGCCAGAA GCAGGAGTTC 
AACCAGGAGC GCCGCATGCT CCTGGAGCTG GTGGGCCCCG AGCTGCAGGG CCTCTACGAC 
GACCGCCAAA TCGAGTTGGA GTTCGTGGAC ATGCACTTCG GCACTGGGGC GCTGGAGGTG 
AACCAGCTGG AGCGGGATCC CTACCTGCTG CAGGACTACC TGCACGAGAT CGACACCTGT 
CATGCCCACA CGAAGAGCGT CTTCTTTCTG GCGCTCATCG GCGACGGCCT CGGACGCCTG 
CCGCTGCCCA CGCGCCTGGA CGAGGACATC TATGCCGCGG TGCTGGCCGA CCCTCAGACA 
ACGGCCGACC AGGAGGCTCT TCTAGCCAGG TGGTACGAGA TGGATCCATC CAAGGTGCAG 
CGTCAGCTGA AGCAGGACTA TCGAGTACTG CCCATGGACG AGTGGCTCAG CGAGTCCCAG 
CAGATGCAAA AGATGCTGGA GCAGGCGCTG CAAGGCTTTG TCCAAGGACC AGGTGCCTCG 
GCGCGATCTG CAGACTTCCT GAAGCGGGTG CAGCAGCTGA GGCGCACGCA GATCGAACGC 
GAAGTGGAGC AGGCCATGGC CCTCTCCAGC GAGAAGATCC TCGCCGTGTT TCGGGAACGG 
GCCGCCCAGT GCAGCAAGCG GGATGTGGAG GCCAATGAGC GGCTTCGGAA GATCAAGGAC 
GAGCTCACCA TGAACCTCTC CACGGACAAT CACACAACCC TAGTGGTGCC AGGCAGTGCC 
TCGTGCGAAA TGATCGACCC GGACAACGAG GACCACGAGT CCTATCTCAG CAAATTCAAG 
AACAAAGTCA CCGACAAGCT GCGTCTGCTA ATCGAGGCGC ACATCACCAA TGATCCCGAT 
GTAATCAAGG GGAGGAAAAA GACTGTTCAG GAAATCTTTC ACGAGCACGC CACTCACCTG 
CGCATCCTGC GCGATCATGT GGAGGGGGCG GCCCTCATCG AGTCGCGGGT GCCACCGCAG 
CTGCGCCAGA ACCTGATGGC GAACTTCAGG AACGGCAGCC GGCATGCGCC GTACTTCATT 
TGCGGCTCGG ATGGCAGCGG CAAGAGCGCC CTGATCAGCA CCCTGTACGG GCAGGTGGCC 
AGCTGGTTCG TCTCGACGAA GGTCCACCGC GTGATCCGCT TCGCGAAGGC CACGCCGCGC 
TCGGCCTACA ACCTGGAGCT GCTGCGCGTG ATCTGCCAGC AGATATCCAT CATCTTCAAC 
ATCCCCGAGG GCTACCTACC CAAGGACGCC TCCTTCGATC CGCTGTACAT CAGCACCTGG 
TTCCAGAACC TGCTGCGGCG CGTCGAGGAC ATGAGCAACG ACGTGCTGTT CCTGTTCATC 
GACGACCTGC ACCTGCTGAA CCCCCTGGAC TGCGACATCG TGACCGCCCT CTCCTGGCTG 
CCCACCTCGC TGCCCTGGAA CGTGCAGATC ATCTGCAGCT CCACCACCCC CGTCGAGCAG 
CTCAAGTTCA CGCCCATGCA GCGGGACCGC TTCAAGTCGG CGGAGTACCA GTTCGACCTC 
AACCTGGGCG AGTACGCCAC GGAGCTGCGG CGACCTCCGC AGTGCGTCTC GGACGTCAGC 
TTCGTCCTGT ACGTGGAGCA GCAGTTCGAC GGCCTGGAGC GCCACTACGG CAGGCAGGCG 
GTGGGCAGCC TGGCCGCCTA CATCACCTGC TCCGAGTACG GGCTGAGCGA GACGGAGCTC 
CTGGAGCTGC TCATGCCCAC CGACGATCCC GAGTCCCTGA TCGACACCAG AAGCGGACAC 
TTTAGCTTCG CCACCTTCAA GAAGATCCAC AGGGAAATGG ACAAGCTCCT GCTGCTGCAC 
GACAAGATCA TGTCCGGCAA GGTGCTCATC CAGTGGCGCC ACAACTACTG CGCCGTGGTG 
GCCAAGCGAC GGTACATGGG CGGCCAGAGG ACGCGCAACC TGCACATGGA GCTGGCCAAC 
CTGTTCTTCC CGCAGGACGA GGACGAGAGC ACGCTGGAGA ACGAGTCCAC CCAGAGCGAG 
AACAAGTCCG TGATTAGCCT CAAGGACACG CTCAGGACAG TCTGCGACCT GCCGCACAAG 
GACAGGGAGC GCGAGAAGGA CAACGTCTCC GCTGGCCGCA AGTCCTCCTC CACCCACCAC 
AACGACGACA CCTCCACCTT CTACAACCCC ATCGCCGCCG ACGTCAGCTA CAGCATGCGG 
CACGTGGAGG AGAGCTGGCA CCACCTGATG CGCTCCGACG ACACGACACG CTTCAAGCAA 
ATCGCAGTCT GCAACTTTGA TTTCCTGCTG GCGGCGGTAC AAACTGTGTC GATCAGCTAT 
TTGCGCTGCC TGATCGAGCA CGTCCGCTGC TATATCCTCG ACAGGGACAT CGAGCTGATC 
TACTACACGA TCCGCAAGTC CAGCGACGTG CTCACGCGCG ACCCCATGCA GCTGGGCTCC 
CAGCTGATCT CCTGGCTGCG TCCGATCAGC GAGCACGACG ACGACGACAA CTCGCTGCTC 
AGCATGACGG TGCGCTCGGC GACGGCCTGG TGCGATGGCT ACGCCGTGCC GCTGCTGGTG 
CCGCTCACGG GATGGCTGCC GGCACCGCTG CCCTCGCAGA TCCGCACCAT GACGGTGTCG 
GGCACGGGCG TCATCCGAGC CGTCTGCCTG GCGCCCTCGA GGCAGCACCT CATCCTGGCC 
ACCAGCTCGG GCGAGGTGCA GCAGTGGCAC ATCATGAGCA ACTCGCTGGA ACACATCTTC 
AAGGGACACA CTGCGGCAGT CACGTGCCTG CTGGTGGCTC CGACCAGCGA GCCGGAGCTC 
CTCCTCACCG GCTCCGAGGA CACCAGCGTG CTCGTCTGGC ACGTCTCGCA GCGCGACCGT 
CGGGCGCACA TCAACGCCCA CACAGCCCCC ATAACGGGCG TGGCGGCCGG GGTCAATAAC 
ACGCTGATCA TCAGCAGCAG CGAGGATGCC ACAATCGCCA TCACGGACCT GGCCAGCGGC 
AAACTGAAAC ACCGAATCAG CCACCATCGC GGCCCTGTGA CCGGGATCCT GGTGGCCGGA 
GCGTGCGATG TCCTGATCTC GGGCAGCCAC GACCGAACCA TCTGCGTGTG GAACCTCGAG 
AACTTTGCGC TGCTGAACAC CATGCAAATG CTGAGCCCCG TCCTGCGCAT CGACATCTCA 
TGGAACTCGG TATTCCTCCT GGCCCTGTGC GAGGACAATG CCCTCTATGT GCGCACTCTG 
GCCACGGGCA AGGAGCTGCA CACGCTCAAG GGCCACAAGT CAAAGATACG GACCATTTCC 
ATTGGCAAGG ACAGTCAGCG CTGCGTGGTG GGATGCGACG ATACGCGAGC CCTGATCTAC 
GACATGCACT CCGGCAAGCT GGTGCGCTCC ATGCCGCCCA ACCCGGGGCC GGTGACCGCC 
GTCTATGCGA TGGAGAACGA CGACTTCCTG GTCACGGTGG GCGGCAACAA GATCACGTTC 
TACTCGTTCC GCAACGAGGA GCTCTACGTG AACCCGTACT CCCGCAACCC GCGCCGCAAG 
CGCAGTCTGA AGCGGCACGC CCAGGCCCAG CGGTCGCCCT CGACGACCCT GCCACCGATC 
ACGTGCTTCG ACCTCTCCCG CGACTCGCAG CTGATGGCCA TCGCCTCAGG CCGCAGCGTG 
CACCTGATGC GCATCAACAC GCCGGAGTAC CAGTGCAGCC TGGAGGGGCA CGCGGGCGTG 
GTCAACTGCC TGAAGTTCGC GCCCAACGGA GAGTTCCTCA CCACGGGATC CGACGATCGG 
CTCGTCCAGG TGTGGAACCT GGCGGCCACC GAGACCATTA ACACCTTCAA GGGGCATGCG 
GCGCCGGTGA TCAAGGTGGT GGTGCTGATG GACTCGCTGC GGGTGATCTC CACGGACCGG 
GACTCACTGC TGCTCGTGTG GATGGCCAGC TCGGGCAACC TGCTGCAGAC CATCCAGGGA 
CCCTACAAGA GCCTGGTGGT GACCAACAAC ATGCGCTTCG CCGCCTCCAG CAACGGGGAC 
AACACTCTGA AGATCTGGTC CCTCACCCAG GAGGACGAGA AGTACTCGGT CTCGCACTCG 
GACGAGATCA CCTGCTTCGA GATCAGCGCC GACAGCATGC ACATCATCAC GGGCTCCCGG 
GACATGTCGC TGAAGGTGTG GCAGTCCACG GGCGGCAAGC TCAGCCAGGT GCTGGTCGGG 
CACAGCGACG CTGTGACCTG TGTGGCCGTC TCGGTGACCA ACAAGACGCA GGTCCTCTCC 
GGCTCCAAGG ACACGAACCT CATCCTGTGG GACCTGCTCA CTGGCGAGGA GGTTCACACG 
CTGGCCGGAC ACTTGGGTCC AGTGATCGGA GTGAAGGTTT CGGCAGATGG CTCCACGGCT 
GTGTCGGGCA GCGATGACAA GACCTTGATC GTGTGGGAGA CGAAGCGCGG ATTGGCCCTG 
ACCTCGCTGC AGATGCACGT GCCCTTCACC CACTTCGACA TCAGCCTGGA GGTGTCGCGG 
GTCCTGGTCC AACTGGTGGA CAGCTACAAC CTCCCGGTGA TCTGTCTGCA CAACACCCCG 
GCGCAGTATG TAAAGCTGCC CACGTACTCG GGACCCACCA AGGATGTGGA TGATCTCCGG 
CCACAGGGCC CGAAGCGCCA GATGAAGCGC CTGCTGAAGA AGGAGGTCTC CCTGGACACG 
TACACTTGGC AGAAGAAGTA CGGCCACCTG ACCTCGTCGG TCATGATGGC CCAGGTTGAC 
GAGCGCCTGA AGCGACGCTT CAGCGTCTCG GCCAGCATGG AGGAGATCTC GAAGATCGCC 
GAGAACAAGA CAGGGGCCTC CAATGCCAAC ATCGGCCCCG AGCAGGCCGC TCTGGCCCAG 
TCGCAGCACT TCGACCAGCT GGAGGCGCTG TGGAACAAGC GGTCTCCGCC CCGACGCCGC 
CACAACGCGG GTCTGTCGAG GCAGACATCG CTGGTGGAGG ACCGGCTCGA GTCGTCGGAC 
GACGACGAGT ACCAGGACGA GCGCGCAGGT ATGTTGTCAG CCTCCTACGA CCACATCCAC 
CTCCTTGCAC GCTTGCCTGG CATCCGCTCC CTGAACGATG CATGGAAGAC AAACAGTCTG 
CGAGTGCCGA GAACCAAGTT CTACGTGAGC ACCCCCCCAC AGACCAACAG CAGCCACCAG 
CTGAACGCGA CCAGTCGGAC AGAACCCCCC TCCTCCGAAC AGAACGCCGA CAGCAGTGCG 
ACCCTCCACC GGGTGGCGCC CGACTTGGTG AGGGATCTGC TACACCCGGT CCCCTCACCG 
GCAGAGTCCC CTCGGCTGAC CACTCTGCGC CAGTTCTTCC ACTGGCCCAG CCTGAAGTCG 
CACCTGATGC GCGGTCAGCG CCAGAGGAAC TGCCTGTCCG TCCAGCCACA CATGCGCCTG 
AATCCGCCGG ACCCCCTCCG ACCCACGCCC ATCATCGTGG TGGAGAACTG TGACAGTCGC 
AATGTGCAGC GCCTGCGCAC CGATCTTGTC CTGCATCCGC AGTACAGTCG CGCCTCATTC 
GGCCACATTG AGGCGGACGA GCACGAGGTT CCCTTGGCCA AGAGGTCATC TTCCTCGAGT 
GGCGGCTACC TCCACCGCCG CTGGCACCTC CTGCGCCACA AATCCCCTTC CGAACGGAGC 
GGTCCCAAAG GCAAAGCCGA AGCCAATTCC AATACAAACT CGAGCTCCCA AGAGTCAACA 
GCCACTTGCA ACGAGTGTGT GGCTCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA15661 ( XM_004444251.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_004444308.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA15661), mRNA.

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CloneID	ODe03972
Clone ID Related Accession (Same CDS sequence)	XM_015184738.1 , XM_004444251.2
Accession Version	XM_004444251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_004444251.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC CCATTGACGA GAGTGTGCTG AACGCACTGA AGGGGGTGAC CTCCAAGCAG 
ACCCGCCTGC CCAAGCCGCT GCTCATCAAG ATCTATGTCG CCAGCCAGAA GCAGGAGTTC 
AACCAGGAGC GCCGCATGCT CCTGGAGCTG GTGGGCCCCG AGCTGCAGGG CCTCTACGAC 
GACCGCCAAA TCGAGTTGGA GTTCGTGGAC ATGCACTTCG GCACTGGGGC GCTGGAGGTG 
AACCAGCTGG AGCGGGATCC CTACCTGCTG CAGGACTACC TGCACGAGAT CGACACCTGT 
CATGCCCACA CGAAGAGCGT CTTCTTTCTG GCGCTCATCG GCGACGGCCT CGGACGCCTG 
CCGCTGCCCA CGCGCCTGGA CGAGGACATC TATGCCGCGG TGCTGGCCGA CCCTCAGACA 
ACGGCCGACC AGGAGGCTCT TCTAGCCAGG TGGTACGAGA TGGATCCATC CAAGGTGCAG 
CGTCAGCTGA AGCAGGACTA TCGAGTACTG CCCATGGACG AGTGGCTCAG CGAGTCCCAG 
CAGATGCAAA AGATGCTGGA GCAGGCGCTG CAAGGCTTTG TCCAAGGACC AGGTGCCTCG 
GCGCGATCTG CAGACTTCCT GAAGCGGGTG CAGCAGCTGA GGCGCACGCA GATCGAACGC 
GAAGTGGAGC AGGCCATGGC CCTCTCCAGC GAGAAGATCC TCGCCGTGTT TCGGGAACGG 
GCCGCCCAGT GCAGCAAGCG GGATGTGGAG GCCAATGAGC GGCTTCGGAA GATCAAGGAC 
GAGCTCACCA TGAACCTCTC CACGGACAAT CACACAACCC TAGTGGTGCC AGGCAGTGCC 
TCGTGCGAAA TGATCGACCC GGACAACGAG GACCACGAGT CCTATCTCAG CAAATTCAAG 
AACAAAGTCA CCGACAAGCT GCGTCTGCTA ATCGAGGCGC ACATCACCAA TGATCCCGAT 
GTAATCAAGG GGAGGAAAAA GACTGTTCAG GAAATCTTTC ACGAGCACGC CACTCACCTG 
CGCATCCTGC GCGATCATGT GGAGGGGGCG GCCCTCATCG AGTCGCGGGT GCCACCGCAG 
CTGCGCCAGA ACCTGATGGC GAACTTCAGG AACGGCAGCC GGCATGCGCC GTACTTCATT 
TGCGGCTCGG ATGGCAGCGG CAAGAGCGCC CTGATCAGCA CCCTGTACGG GCAGGTGGCC 
AGCTGGTTCG TCTCGACGAA GGTCCACCGC GTGATCCGCT TCGCGAAGGC CACGCCGCGC 
TCGGCCTACA ACCTGGAGCT GCTGCGCGTG ATCTGCCAGC AGATATCCAT CATCTTCAAC 
ATCCCCGAGG GCTACCTACC CAAGGACGCC TCCTTCGATC CGCTGTACAT CAGCACCTGG 
TTCCAGAACC TGCTGCGGCG CGTCGAGGAC ATGAGCAACG ACGTGCTGTT CCTGTTCATC 
GACGACCTGC ACCTGCTGAA CCCCCTGGAC TGCGACATCG TGACCGCCCT CTCCTGGCTG 
CCCACCTCGC TGCCCTGGAA CGTGCAGATC ATCTGCAGCT CCACCACCCC CGTCGAGCAG 
CTCAAGTTCA CGCCCATGCA GCGGGACCGC TTCAAGTCGG CGGAGTACCA GTTCGACCTC 
AACCTGGGCG AGTACGCCAC GGAGCTGCGG CGACCTCCGC AGTGCGTCTC GGACGTCAGC 
TTCGTCCTGT ACGTGGAGCA GCAGTTCGAC GGCCTGGAGC GCCACTACGG CAGGCAGGCG 
GTGGGCAGCC TGGCCGCCTA CATCACCTGC TCCGAGTACG GGCTGAGCGA GACGGAGCTC 
CTGGAGCTGC TCATGCCCAC CGACGATCCC GAGTCCCTGA TCGACACCAG AAGCGGACAC 
TTTAGCTTCG CCACCTTCAA GAAGATCCAC AGGGAAATGG ACAAGCTCCT GCTGCTGCAC 
GACAAGATCA TGTCCGGCAA GGTGCTCATC CAGTGGCGCC ACAACTACTG CGCCGTGGTG 
GCCAAGCGAC GGTACATGGG CGGCCAGAGG ACGCGCAACC TGCACATGGA GCTGGCCAAC 
CTGTTCTTCC CGCAGGACGA GGACGAGAGC ACGCTGGAGA ACGAGTCCAC CCAGAGCGAG 
AACAAGTCCG TGATTAGCCT CAAGGACACG CTCAGGACAG TCTGCGACCT GCCGCACAAG 
GACAGGGAGC GCGAGAAGGA CAACGTCTCC GCTGGCCGCA AGTCCTCCTC CACCCACCAC 
AACGACGACA CCTCCACCTT CTACAACCCC ATCGCCGCCG ACGTCAGCTA CAGCATGCGG 
CACGTGGAGG AGAGCTGGCA CCACCTGATG CGCTCCGACG ACACGACACG CTTCAAGCAA 
ATCGCAGTCT GCAACTTTGA TTTCCTGCTG GCGGCGGTAC AAACTGTGTC GATCAGCTAT 
TTGCGCTGCC TGATCGAGCA CGTCCGCTGC TATATCCTCG ACAGGGACAT CGAGCTGATC 
TACTACACGA TCCGCAAGTC CAGCGACGTG CTCACGCGCG ACCCCATGCA GCTGGGCTCC 
CAGCTGATCT CCTGGCTGCG TCCGATCAGC GAGCACGACG ACGACGACAA CTCGCTGCTC 
AGCATGACGG TGCGCTCGGC GACGGCCTGG TGCGATGGCT ACGCCGTGCC GCTGCTGGTG 
CCGCTCACGG GATGGCTGCC GGCACCGCTG CCCTCGCAGA TCCGCACCAT GACGGTGTCG 
GGCACGGGCG TCATCCGAGC CGTCTGCCTG GCGCCCTCGA GGCAGCACCT CATCCTGGCC 
ACCAGCTCGG GCGAGGTGCA GCAGTGGCAC ATCATGAGCA ACTCGCTGGA ACACATCTTC 
AAGGGACACA CTGCGGCAGT CACGTGCCTG CTGGTGGCTC CGACCAGCGA GCCGGAGCTC 
CTCCTCACCG GCTCCGAGGA CACCAGCGTG CTCGTCTGGC ACGTCTCGCA GCGCGACCGT 
CGGGCGCACA TCAACGCCCA CACAGCCCCC ATAACGGGCG TGGCGGCCGG GGTCAATAAC 
ACGCTGATCA TCAGCAGCAG CGAGGATGCC ACAATCGCCA TCACGGACCT GGCCAGCGGC 
AAACTGAAAC ACCGAATCAG CCACCATCGC GGCCCTGTGA CCGGGATCCT GGTGGCCGGA 
GCGTGCGATG TCCTGATCTC GGGCAGCCAC GACCGAACCA TCTGCGTGTG GAACCTCGAG 
AACTTTGCGC TGCTGAACAC CATGCAAATG CTGAGCCCCG TCCTGCGCAT CGACATCTCA 
TGGAACTCGG TATTCCTCCT GGCCCTGTGC GAGGACAATG CCCTCTATGT GCGCACTCTG 
GCCACGGGCA AGGAGCTGCA CACGCTCAAG GGCCACAAGT CAAAGATACG GACCATTTCC 
ATTGGCAAGG ACAGTCAGCG CTGCGTGGTG GGATGCGACG ATACGCGAGC CCTGATCTAC 
GACATGCACT CCGGCAAGCT GGTGCGCTCC ATGCCGCCCA ACCCGGGGCC GGTGACCGCC 
GTCTATGCGA TGGAGAACGA CGACTTCCTG GTCACGGTGG GCGGCAACAA GATCACGTTC 
TACTCGTTCC GCAACGAGGA GCTCTACGTG AACCCGTACT CCCGCAACCC GCGCCGCAAG 
CGCAGTCTGA AGCGGCACGC CCAGGCCCAG CGGTCGCCCT CGACGACCCT GCCACCGATC 
ACGTGCTTCG ACCTCTCCCG CGACTCGCAG CTGATGGCCA TCGCCTCAGG CCGCAGCGTG 
CACCTGATGC GCATCAACAC GCCGGAGTAC CAGTGCAGCC TGGAGGGGCA CGCGGGCGTG 
GTCAACTGCC TGAAGTTCGC GCCCAACGGA GAGTTCCTCA CCACGGGATC CGACGATCGG 
CTCGTCCAGG TGTGGAACCT GGCGGCCACC GAGACCATTA ACACCTTCAA GGGGCATGCG 
GCGCCGGTGA TCAAGGTGGT GGTGCTGATG GACTCGCTGC GGGTGATCTC CACGGACCGG 
GACTCACTGC TGCTCGTGTG GATGGCCAGC TCGGGCAACC TGCTGCAGAC CATCCAGGGA 
CCCTACAAGA GCCTGGTGGT GACCAACAAC ATGCGCTTCG CCGCCTCCAG CAACGGGGAC 
AACACTCTGA AGATCTGGTC CCTCACCCAG GAGGACGAGA AGTACTCGGT CTCGCACTCG 
GACGAGATCA CCTGCTTCGA GATCAGCGCC GACAGCATGC ACATCATCAC GGGCTCCCGG 
GACATGTCGC TGAAGGTGTG GCAGTCCACG GGCGGCAAGC TCAGCCAGGT GCTGGTCGGG 
CACAGCGACG CTGTGACCTG TGTGGCCGTC TCGGTGACCA ACAAGACGCA GGTCCTCTCC 
GGCTCCAAGG ACACGAACCT CATCCTGTGG GACCTGCTCA CTGGCGAGGA GGTTCACACG 
CTGGCCGGAC ACTTGGGTCC AGTGATCGGA GTGAAGGTTT CGGCAGATGG CTCCACGGCT 
GTGTCGGGCA GCGATGACAA GACCTTGATC GTGTGGGAGA CGAAGCGCGG ATTGGCCCTG 
ACCTCGCTGC AGATGCACGT GCCCTTCACC CACTTCGACA TCAGCCTGGA GGTGTCGCGG 
GTCCTGGTCC AACTGGTGGA CAGCTACAAC CTCCCGGTGA TCTGTCTGCA CAACACCCCG 
GCGCAGTATG TAAAGCTGCC CACGTACTCG GGACCCACCA AGGATGTGGA TGATCTCCGG 
CCACAGGGCC CGAAGCGCCA GATGAAGCGC CTGCTGAAGA AGGAGGTCTC CCTGGACACG 
TACACTTGGC AGAAGAAGTA CGGCCACCTG ACCTCGTCGG TCATGATGGC CCAGGTTGAC 
GAGCGCCTGA AGCGACGCTT CAGCGTCTCG GCCAGCATGG AGGAGATCTC GAAGATCGCC 
GAGAACAAGA CAGGGGCCTC CAATGCCAAC ATCGGCCCCG AGCAGGCCGC TCTGGCCCAG 
TCGCAGCACT TCGACCAGCT GGAGGCGCTG TGGAACAAGC GGTCTCCGCC CCGACGCCGC 
CACAACGCGG GTCTGTCGAG GCAGACATCG CTGGTGGAGG ACCGGCTCGA GTCGTCGGAC 
GACGACGAGT ACCAGGACGA GCGCGCAGGT ATGTTGTCAG CCTCCTACGA CCACATCCAC 
CTCCTTGCAC GCTTGCCTGG CATCCGCTCC CTGAACGATG CATGGAAGAC AAACAGTCTG 
CGAGTGCCGA GAACCAAGTT CTACGTGAGC ACCCCCCCAC AGACCAACAG CAGCCACCAG 
CTGAACGCGA CCAGTCGGAC AGAACCCCCC TCCTCCGAAC AGAACGCCGA CAGCAGTGCG 
ACCCTCCACC GGGTGGCGCC CGACTTGGTG AGGGATCTGC TACACCCGGT CCCCTCACCG 
GCAGAGTCCC CTCGGCTGAC CACTCTGCGC CAGTTCTTCC ACTGGCCCAG CCTGAAGTCG 
CACCTGATGC GCGGTCAGCG CCAGAGGAAC TGCCTGTCCG TCCAGCCACA CATGCGCCTG 
AATCCGCCGG ACCCCCTCCG ACCCACGCCC ATCATCGTGG TGGAGAACTG TGACAGTCGC 
AATGTGCAGC GCCTGCGCAC CGATCTTGTC CTGCATCCGC AGTACAGTCG CGCCTCATTC 
GGCCACATTG AGGCGGACGA GCACGAGGTT CCCTTGGCCA AGAGGTCATC TTCCTCGAGT 
GGCGGCTACC TCCACCGCCG CTGGCACCTC CTGCGCCACA AATCCCCTTC CGAACGGAGC 
GGTCCCAAAG GCAAAGCCGA AGCCAATTCC AATACAAACT CGAGCTCCCA AGAGTCAACA 
GCCACTTGCA ACGAGTGTGT GGCTCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004444308.1
CDS	550..6339
Translation	MAAPIDESVLNALKGVTSKQTRLPKPLLIKIYVASQKQEFNQERRMLLELVGPELQGLYDDRQIELEFVDMHFGTGALEVNQLERDPYLLQDYLHEIDTCHAHTKSVFFLALIGDGLGRLPLPTRLDEDIYAAVLADPQTTADQEALLARWYEMDPSKVQRQLKQDYRVLPMDEWLSESQQMQKMLEQALQGFVQGPGASARSADFLKRVQQLRRTQIEREVEQAMALSSEKILAVFRERAAQCSKRDVEANERLRKIKDELTMNLSTDNHTTLVVPGSASCEMIDPDNEDHESYLSKFKNKVTDKLRLLIEAHITNDPDVIKGRKKTVQEIFHEHATHLRILRDHVEGAALIESRVPPQLRQNLMANFRNGSRHAPYFICGSDGSGKSALISTLYGQVASWFVSTKVHRVIRFAKATPRSAYNLELLRVICQQISIIFNIPEGYLPKDASFDPLYISTWFQNLLRRVEDMSNDVLFLFIDDLHLLNPLDCDIVTALSWLPTSLPWNVQIICSSTTPVEQLKFTPMQRDRFKSAEYQFDLNLGEYATELRRPPQCVSDVSFVLYVEQQFDGLERHYGRQAVGSLAAYITCSEYGLSETELLELLMPTDDPESLIDTRSGHFSFATFKKIHREMDKLLLLHDKIMSGKVLIQWRHNYCAVVAKRRYMGGQRTRNLHMELANLFFPQDEDESTLENESTQSENKSVISLKDTLRTVCDLPHKDREREKDNVSAGRKSSSTHHNDDTSTFYNPIAADVSYSMRHVEESWHHLMRSDDTTRFKQIAVCNFDFLLAAVQTVSISYLRCLIEHVRCYILDRDIELIYYTIRKSSDVLTRDPMQLGSQLISWLRPISEHDDDDNSLLSMTVRSATAWCDGYAVPLLVPLTGWLPAPLPSQIRTMTVSGTGVIRAVCLAPSRQHLILATSSGEVQQWHIMSNSLEHIFKGHTAAVTCLLVAPTSEPELLLTGSEDTSVLVWHVSQRDRRAHINAHTAPITGVAAGVNNTLIISSSEDATIAITDLASGKLKHRISHHRGPVTGILVAGACDVLISGSHDRTICVWNLENFALLNTMQMLSPVLRIDISWNSVFLLALCEDNALYVRTLATGKELHTLKGHKSKIRTISIGKDSQRCVVGCDDTRALIYDMHSGKLVRSMPPNPGPVTAVYAMENDDFLVTVGGNKITFYSFRNEELYVNPYSRNPRRKRSLKRHAQAQRSPSTTLPPITCFDLSRDSQLMAIASGRSVHLMRINTPEYQCSLEGHAGVVNCLKFAPNGEFLTTGSDDRLVQVWNLAATETINTFKGHAAPVIKVVVLMDSLRVISTDRDSLLLVWMASSGNLLQTIQGPYKSLVVTNNMRFAASSNGDNTLKIWSLTQEDEKYSVSHSDEITCFEISADSMHIITGSRDMSLKVWQSTGGKLSQVLVGHSDAVTCVAVSVTNKTQVLSGSKDTNLILWDLLTGEEVHTLAGHLGPVIGVKVSADGSTAVSGSDDKTLIVWETKRGLALTSLQMHVPFTHFDISLEVSRVLVQLVDSYNLPVICLHNTPAQYVKLPTYSGPTKDVDDLRPQGPKRQMKRLLKKEVSLDTYTWQKKYGHLTSSVMMAQVDERLKRRFSVSASMEEISKIAENKTGASNANIGPEQAALAQSQHFDQLEALWNKRSPPRRRHNAGLSRQTSLVEDRLESSDDDEYQDERAGMLSASYDHIHLLARLPGIRSLNDAWKTNSLRVPRTKFYVSTPPQTNSSHQLNATSRTEPPSSEQNADSSATLHRVAPDLVRDLLHPVPSPAESPRLTTLRQFFHWPSLKSHLMRGQRQRNCLSVQPHMRLNPPDPLRPTPIIVVENCDSRNVQRLRTDLVLHPQYSRASFGHIEADEHEVPLAKRSSSSSGGYLHRRWHLLRHKSPSERSGPKGKAEANSNTNSSSQESTATCNECVAQ

Target ORF information:

RefSeq Version	XM_004444251.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA15661), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004444251.2

ORF Insert Sequence:

ATGGCCGCGC CCATTGACGA GAGTGTGCTG AACGCACTGA AGGGGGTGAC CTCCAAGCAG 
ACCCGCCTGC CCAAGCCGCT GCTCATCAAG ATCTATGTCG CCAGCCAGAA GCAGGAGTTC 
AACCAGGAGC GCCGCATGCT CCTGGAGCTG GTGGGCCCCG AGCTGCAGGG CCTCTACGAC 
GACCGCCAAA TCGAGTTGGA GTTCGTGGAC ATGCACTTCG GCACTGGGGC GCTGGAGGTG 
AACCAGCTGG AGCGGGATCC CTACCTGCTG CAGGACTACC TGCACGAGAT CGACACCTGT 
CATGCCCACA CGAAGAGCGT CTTCTTTCTG GCGCTCATCG GCGACGGCCT CGGACGCCTG 
CCGCTGCCCA CGCGCCTGGA CGAGGACATC TATGCCGCGG TGCTGGCCGA CCCTCAGACA 
ACGGCCGACC AGGAGGCTCT TCTAGCCAGG TGGTACGAGA TGGATCCATC CAAGGTGCAG 
CGTCAGCTGA AGCAGGACTA TCGAGTACTG CCCATGGACG AGTGGCTCAG CGAGTCCCAG 
CAGATGCAAA AGATGCTGGA GCAGGCGCTG CAAGGCTTTG TCCAAGGACC AGGTGCCTCG 
GCGCGATCTG CAGACTTCCT GAAGCGGGTG CAGCAGCTGA GGCGCACGCA GATCGAACGC 
GAAGTGGAGC AGGCCATGGC CCTCTCCAGC GAGAAGATCC TCGCCGTGTT TCGGGAACGG 
GCCGCCCAGT GCAGCAAGCG GGATGTGGAG GCCAATGAGC GGCTTCGGAA GATCAAGGAC 
GAGCTCACCA TGAACCTCTC CACGGACAAT CACACAACCC TAGTGGTGCC AGGCAGTGCC 
TCGTGCGAAA TGATCGACCC GGACAACGAG GACCACGAGT CCTATCTCAG CAAATTCAAG 
AACAAAGTCA CCGACAAGCT GCGTCTGCTA ATCGAGGCGC ACATCACCAA TGATCCCGAT 
GTAATCAAGG GGAGGAAAAA GACTGTTCAG GAAATCTTTC ACGAGCACGC CACTCACCTG 
CGCATCCTGC GCGATCATGT GGAGGGGGCG GCCCTCATCG AGTCGCGGGT GCCACCGCAG 
CTGCGCCAGA ACCTGATGGC GAACTTCAGG AACGGCAGCC GGCATGCGCC GTACTTCATT 
TGCGGCTCGG ATGGCAGCGG CAAGAGCGCC CTGATCAGCA CCCTGTACGG GCAGGTGGCC 
AGCTGGTTCG TCTCGACGAA GGTCCACCGC GTGATCCGCT TCGCGAAGGC CACGCCGCGC 
TCGGCCTACA ACCTGGAGCT GCTGCGCGTG ATCTGCCAGC AGATATCCAT CATCTTCAAC 
ATCCCCGAGG GCTACCTACC CAAGGACGCC TCCTTCGATC CGCTGTACAT CAGCACCTGG 
TTCCAGAACC TGCTGCGGCG CGTCGAGGAC ATGAGCAACG ACGTGCTGTT CCTGTTCATC 
GACGACCTGC ACCTGCTGAA CCCCCTGGAC TGCGACATCG TGACCGCCCT CTCCTGGCTG 
CCCACCTCGC TGCCCTGGAA CGTGCAGATC ATCTGCAGCT CCACCACCCC CGTCGAGCAG 
CTCAAGTTCA CGCCCATGCA GCGGGACCGC TTCAAGTCGG CGGAGTACCA GTTCGACCTC 
AACCTGGGCG AGTACGCCAC GGAGCTGCGG CGACCTCCGC AGTGCGTCTC GGACGTCAGC 
TTCGTCCTGT ACGTGGAGCA GCAGTTCGAC GGCCTGGAGC GCCACTACGG CAGGCAGGCG 
GTGGGCAGCC TGGCCGCCTA CATCACCTGC TCCGAGTACG GGCTGAGCGA GACGGAGCTC 
CTGGAGCTGC TCATGCCCAC CGACGATCCC GAGTCCCTGA TCGACACCAG AAGCGGACAC 
TTTAGCTTCG CCACCTTCAA GAAGATCCAC AGGGAAATGG ACAAGCTCCT GCTGCTGCAC 
GACAAGATCA TGTCCGGCAA GGTGCTCATC CAGTGGCGCC ACAACTACTG CGCCGTGGTG 
GCCAAGCGAC GGTACATGGG CGGCCAGAGG ACGCGCAACC TGCACATGGA GCTGGCCAAC 
CTGTTCTTCC CGCAGGACGA GGACGAGAGC ACGCTGGAGA ACGAGTCCAC CCAGAGCGAG 
AACAAGTCCG TGATTAGCCT CAAGGACACG CTCAGGACAG TCTGCGACCT GCCGCACAAG 
GACAGGGAGC GCGAGAAGGA CAACGTCTCC GCTGGCCGCA AGTCCTCCTC CACCCACCAC 
AACGACGACA CCTCCACCTT CTACAACCCC ATCGCCGCCG ACGTCAGCTA CAGCATGCGG 
CACGTGGAGG AGAGCTGGCA CCACCTGATG CGCTCCGACG ACACGACACG CTTCAAGCAA 
ATCGCAGTCT GCAACTTTGA TTTCCTGCTG GCGGCGGTAC AAACTGTGTC GATCAGCTAT 
TTGCGCTGCC TGATCGAGCA CGTCCGCTGC TATATCCTCG ACAGGGACAT CGAGCTGATC 
TACTACACGA TCCGCAAGTC CAGCGACGTG CTCACGCGCG ACCCCATGCA GCTGGGCTCC 
CAGCTGATCT CCTGGCTGCG TCCGATCAGC GAGCACGACG ACGACGACAA CTCGCTGCTC 
AGCATGACGG TGCGCTCGGC GACGGCCTGG TGCGATGGCT ACGCCGTGCC GCTGCTGGTG 
CCGCTCACGG GATGGCTGCC GGCACCGCTG CCCTCGCAGA TCCGCACCAT GACGGTGTCG 
GGCACGGGCG TCATCCGAGC CGTCTGCCTG GCGCCCTCGA GGCAGCACCT CATCCTGGCC 
ACCAGCTCGG GCGAGGTGCA GCAGTGGCAC ATCATGAGCA ACTCGCTGGA ACACATCTTC 
AAGGGACACA CTGCGGCAGT CACGTGCCTG CTGGTGGCTC CGACCAGCGA GCCGGAGCTC 
CTCCTCACCG GCTCCGAGGA CACCAGCGTG CTCGTCTGGC ACGTCTCGCA GCGCGACCGT 
CGGGCGCACA TCAACGCCCA CACAGCCCCC ATAACGGGCG TGGCGGCCGG GGTCAATAAC 
ACGCTGATCA TCAGCAGCAG CGAGGATGCC ACAATCGCCA TCACGGACCT GGCCAGCGGC 
AAACTGAAAC ACCGAATCAG CCACCATCGC GGCCCTGTGA CCGGGATCCT GGTGGCCGGA 
GCGTGCGATG TCCTGATCTC GGGCAGCCAC GACCGAACCA TCTGCGTGTG GAACCTCGAG 
AACTTTGCGC TGCTGAACAC CATGCAAATG CTGAGCCCCG TCCTGCGCAT CGACATCTCA 
TGGAACTCGG TATTCCTCCT GGCCCTGTGC GAGGACAATG CCCTCTATGT GCGCACTCTG 
GCCACGGGCA AGGAGCTGCA CACGCTCAAG GGCCACAAGT CAAAGATACG GACCATTTCC 
ATTGGCAAGG ACAGTCAGCG CTGCGTGGTG GGATGCGACG ATACGCGAGC CCTGATCTAC 
GACATGCACT CCGGCAAGCT GGTGCGCTCC ATGCCGCCCA ACCCGGGGCC GGTGACCGCC 
GTCTATGCGA TGGAGAACGA CGACTTCCTG GTCACGGTGG GCGGCAACAA GATCACGTTC 
TACTCGTTCC GCAACGAGGA GCTCTACGTG AACCCGTACT CCCGCAACCC GCGCCGCAAG 
CGCAGTCTGA AGCGGCACGC CCAGGCCCAG CGGTCGCCCT CGACGACCCT GCCACCGATC 
ACGTGCTTCG ACCTCTCCCG CGACTCGCAG CTGATGGCCA TCGCCTCAGG CCGCAGCGTG 
CACCTGATGC GCATCAACAC GCCGGAGTAC CAGTGCAGCC TGGAGGGGCA CGCGGGCGTG 
GTCAACTGCC TGAAGTTCGC GCCCAACGGA GAGTTCCTCA CCACGGGATC CGACGATCGG 
CTCGTCCAGG TGTGGAACCT GGCGGCCACC GAGACCATTA ACACCTTCAA GGGGCATGCG 
GCGCCGGTGA TCAAGGTGGT GGTGCTGATG GACTCGCTGC GGGTGATCTC CACGGACCGG 
GACTCACTGC TGCTCGTGTG GATGGCCAGC TCGGGCAACC TGCTGCAGAC CATCCAGGGA 
CCCTACAAGA GCCTGGTGGT GACCAACAAC ATGCGCTTCG CCGCCTCCAG CAACGGGGAC 
AACACTCTGA AGATCTGGTC CCTCACCCAG GAGGACGAGA AGTACTCGGT CTCGCACTCG 
GACGAGATCA CCTGCTTCGA GATCAGCGCC GACAGCATGC ACATCATCAC GGGCTCCCGG 
GACATGTCGC TGAAGGTGTG GCAGTCCACG GGCGGCAAGC TCAGCCAGGT GCTGGTCGGG 
CACAGCGACG CTGTGACCTG TGTGGCCGTC TCGGTGACCA ACAAGACGCA GGTCCTCTCC 
GGCTCCAAGG ACACGAACCT CATCCTGTGG GACCTGCTCA CTGGCGAGGA GGTTCACACG 
CTGGCCGGAC ACTTGGGTCC AGTGATCGGA GTGAAGGTTT CGGCAGATGG CTCCACGGCT 
GTGTCGGGCA GCGATGACAA GACCTTGATC GTGTGGGAGA CGAAGCGCGG ATTGGCCCTG 
ACCTCGCTGC AGATGCACGT GCCCTTCACC CACTTCGACA TCAGCCTGGA GGTGTCGCGG 
GTCCTGGTCC AACTGGTGGA CAGCTACAAC CTCCCGGTGA TCTGTCTGCA CAACACCCCG 
GCGCAGTATG TAAAGCTGCC CACGTACTCG GGACCCACCA AGGATGTGGA TGATCTCCGG 
CCACAGGGCC CGAAGCGCCA GATGAAGCGC CTGCTGAAGA AGGAGGTCTC CCTGGACACG 
TACACTTGGC AGAAGAAGTA CGGCCACCTG ACCTCGTCGG TCATGATGGC CCAGGTTGAC 
GAGCGCCTGA AGCGACGCTT CAGCGTCTCG GCCAGCATGG AGGAGATCTC GAAGATCGCC 
GAGAACAAGA CAGGGGCCTC CAATGCCAAC ATCGGCCCCG AGCAGGCCGC TCTGGCCCAG 
TCGCAGCACT TCGACCAGCT GGAGGCGCTG TGGAACAAGC GGTCTCCGCC CCGACGCCGC 
CACAACGCGG GTCTGTCGAG GCAGACATCG CTGGTGGAGG ACCGGCTCGA GTCGTCGGAC 
GACGACGAGT ACCAGGACGA GCGCGCAGGT ATGTTGTCAG CCTCCTACGA CCACATCCAC 
CTCCTTGCAC GCTTGCCTGG CATCCGCTCC CTGAACGATG CATGGAAGAC AAACAGTCTG 
CGAGTGCCGA GAACCAAGTT CTACGTGAGC ACCCCCCCAC AGACCAACAG CAGCCACCAG 
CTGAACGCGA CCAGTCGGAC AGAACCCCCC TCCTCCGAAC AGAACGCCGA CAGCAGTGCG 
ACCCTCCACC GGGTGGCGCC CGACTTGGTG AGGGATCTGC TACACCCGGT CCCCTCACCG 
GCAGAGTCCC CTCGGCTGAC CACTCTGCGC CAGTTCTTCC ACTGGCCCAG CCTGAAGTCG 
CACCTGATGC GCGGTCAGCG CCAGAGGAAC TGCCTGTCCG TCCAGCCACA CATGCGCCTG 
AATCCGCCGG ACCCCCTCCG ACCCACGCCC ATCATCGTGG TGGAGAACTG TGACAGTCGC 
AATGTGCAGC GCCTGCGCAC CGATCTTGTC CTGCATCCGC AGTACAGTCG CGCCTCATTC 
GGCCACATTG AGGCGGACGA GCACGAGGTT CCCTTGGCCA AGAGGTCATC TTCCTCGAGT 
GGCGGCTACC TCCACCGCCG CTGGCACCTC CTGCGCCACA AATCCCCTTC CGAACGGAGC 
GGTCCCAAAG GCAAAGCCGA AGCCAATTCC AATACAAACT CGAGCTCCCA AGAGTCAACA 
GCCACTTGCA ACGAGTGTGT GGCTCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA15661 ( XM_015184739.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015040225.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA15661), mRNA.

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CloneID	ODe23190
Clone ID Related Accession (Same CDS sequence)	XM_015184739.1
Accession Version	XM_015184739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC CCATTGACGA GAGTGTGCTG AACGCACTGA AGGGGGTGAC CTCCAAGCAG 
ACCCGCCTGC CCAAGCCGCT GCTCATCAAG ATCTATGTCG CCAGCCAGAA GCAGGAGTTC 
AACCAGGAGC GCCGCATGCT CCTGGAGCTG GTGGGCCCCG AGCTGCAGGG CCTCTACGAC 
GACCGCCAAA TCGAGTTGGA GTTCGTGGAC ATGCACTTCG GCACTGGGGC GCTGGAGGTG 
AACCAGCTGG AGCGGGATCC CTACCTGCTG CAGGACTACC TGCACGAGAT CGACACCTGT 
CATGCCCACA CGAAGAGCGT CTTCTTTCTG GCGCTCATCG GCGACGGCCT CGGACGCCTG 
CCGCTGCCCA CGCGCCTGGA CGAGGACATC TATGCCGCGG TGCTGGCCGA CCCTCAGACA 
ACGGCCGACC AGGAGGCTCT TCTAGCCAGG TGGTACGAGA TGGATCCATC CAAGGTGCAG 
CGTCAGCTGA AGCAGGACTA TCGAGTACTG CCCATGGACG AGTGGCTCAG CGAGTCCCAG 
CAGATGCAAA AGATGCTGGA GCAGGCGCTG CAAGGCTTTG TCCAAGGACC AGGTGCCTCG 
GCGCGATCTG CAGACTTCCT GAAGCGGGTG CAGCAGCTGA GGCGCACGCA GATCGAACGC 
GAAGTGGAGC AGGCCATGGC CCTCTCCAGC GAGAAGATCC TCGCCGTGTT TCGGGAACGG 
GCCGCCCAGT GCAGCAAGCG GGATGTGGAG GCCAATGAGC GGCTTCGGAA GATCAAGGAC 
GAGCTCACCA TGAACCTCTC CACGGACAAT CACACAACCC TAGTGGTGCC AGGCAGTGCC 
TCGTGCGAAA TGATCGACCC GGACAACGAG GACCACGAGT CCTATCTCAG CAAATTCAAG 
AACAAAGTCA CCGACAAGCT GCGTCTGCTA ATCGAGGCGC ACATCACCAA TGATCCCGAT 
GTAATCAAGG GGAGGAAAAA GACTGTTCAG GAAATCTTTC ACGAGCACGC CACTCACCTG 
CGCATCCTGC GCGATCATGT GGAGGGGGCG GCCCTCATCG AGTCGCGGGT GCCACCGCAG 
CTGCGCCAGA ACCTGATGGC GAACTTCAGG AACGGCAGCC GGCATGCGCC GTACTTCATT 
TGCGGCTCGG ATGGCAGCGG CAAGAGCGCC CTGATCAGCA CCCTGTACGG GCAGGTGGCC 
AGCTGGTTCG TCTCGACGAA GGTCCACCGC GTGATCCGCT TCGCGAAGGC CACGCCGCGC 
TCGGCCTACA ACCTGGAGCT GCTGCGCGTG ATCTGCCAGC AGATATCCAT CATCTTCAAC 
ATCCCCGAGG GCTACCTACC CAAGGACGCC TCCTTCGATC CGCTGTACAT CAGCACCTGG 
TTCCAGAACC TGCTGCGGCG CGTCGAGGAC ATGAGCAACG ACGTGCTGTT CCTGTTCATC 
GACGACCTGC ACCTGCTGAA CCCCCTGGAC TGCGACATCG TGACCGCCCT CTCCTGGCTG 
CCCACCTCGC TGCCCTGGAA CGTGCAGATC ATCTGCAGCT CCACCACCCC CGTCGAGCAG 
CTCAAGTTCA CGCCCATGCA GCGGGACCGC TTCAAGTCGG CGGAGTACCA GTTCGACCTC 
AACCTGGGCG AGTACGCCAC GGAGCTGCGG CGACCTCCGC AGTGCGTCTC GGACGTCAGC 
TTCGTCCTGT ACGTGGAGCA GCAGTTCGAC GGCCTGGAGC GCCACTACGG CAGGCAGGCG 
GTGGGCAGCC TGGCCGCCTA CATCACCTGC TCCGAGTACG GGCTGAGCGA GACGGAGCTC 
CTGGAGCTGC TCATGCCCAC CGACGATCCC GAGTCCCTGA TCGACACCAG AAGCGGACAC 
TTTAGCTTCG CCACCTTCAA GAAGATCCAC AGGGAAATGG ACAAGCTCCT GCTGCTGCAC 
GACAAGATCA TGTCCGGCAA GGTGCTCATC CAGTGGCGCC ACAACTACTG CGCCGTGGTG 
GCCAAGCGAC GGTACATGGG CGGCCAGAGG ACGCGCAACC TGCACATGGA GCTGGCCAAC 
CTGTTCTTCC CGCAGGACGA GGACGAGAGC ACGCTGGAGA ACGAGTCCAC CCAGAGCGAG 
AACAAGTCCG TGATTAGCCT CAAGGACACG CTCAGGACAG TCTGCGACCT GCCGCACAAG 
GACAGGGAGC GCGAGAAGGA CAACGTCTCC GCTGGCCGCA AGTCCTCCTC CACCCACCAC 
AACGACGACA CCTCCACCTT CTACAACCCC ATCGCCGCCG ACGTCAGCTA CAGCATGCGG 
CACGTGGAGG AGAGCTGGCA CCACCTGATG CGCTCCGACG ACACGACACG CTTCAAGCAA 
ATCGCAGTCT GCAACTTTGA TTTCCTGCTG GCGGCGGTAC AAACTGTGTC GATCAGCTAT 
TTGCGCTGCC TGATCGAGCA CGTCCGCTGC TATATCCTCG ACAGGGACAT CGAGCTGATC 
TACTACACGA TCCGCAAGTC CAGCGACGTG CTCACGCGCG ACCCCATGCA GCTGGGCTCC 
CAGCTGATCT CCTGGCTGCG TCCGATCAGC GAGCACGACG ACGACGACAA CTCGCTGCTC 
AGCATGACGG TGCGCTCGGC GACGGCCTGG TGCGATGGCT ACGCCGTGCC GCTGCTGGTG 
CCGCTCACGG GATGGCTGCC GGCACCGCTG CCCTCGCAGA TCCGCACCAT GACGGTGTCG 
GGCACGGGCG TCATCCGAGC CGTCTGCCTG GCGCCCTCGA GGCAGCACCT CATCCTGGCC 
ACCAGCTCGG GCGAGGTGCA GCAGTGGCAC ATCATGAGCA ACTCGCTGGA ACACATCTTC 
AAGGGACACA CTGCGGCAGT CACGTGCCTG CTGGTGGCTC CGACCAGCGA GCCGGAGCTC 
CTCCTCACCG GCTCCGAGGA CACCAGCGTG CTCGTCTGGC ACGTCTCGCA GCGCGACCGT 
CGGGCGCACA TCAACGCCCA CACAGCCCCC ATAACGGGCG TGGCGGCCGG GGTCAATAAC 
ACGCTGATCA TCAGCAGCAG CGAGGATGCC ACAATCGCCA TCACGGACCT GGCCAGCGGC 
AAACTGAAAC ACCGAATCAG CCACCATCGC GGCCCTGTGA CCGGGATCCT GGTGGCCGGA 
GCGTGCGATG TCCTGATCTC GGGCAGCCAC GACCGAACCA TCTGCGTGTG GAACCTCGAG 
AACTTTGCGC TGCTGAACAC CATGCAAATG CTGAGCCCCG TCCTGCGCAT CGACATCTCA 
TGGAACTCGG TATTCCTCCT GGCCCTGTGC GAGGACAATG CCCTCTATGT GCGCACTCTG 
GCCACGGGCA AGGAGCTGCA CACGCTCAAG GGCCACAAGT CAAAGATACG GACCATTTCC 
ATTGGCAAGG ACAGTCAGCG CTGCGTGGTG GGATGCGACG ATACGCGAGC CCTGATCTAC 
GACATGCACT CCGGCAAGCT GGTGCGCTCC ATGCCGCCCA ACCCGGGGCC GGTGACCGCC 
GTCTATGCGA TGGAGAACGA CGACTTCCTG GTCACGGTGG GCGGCAACAA GATCACGTTC 
TACTCGTTCC GCAACGAGGA GCTCTACGTG AACCCGTACT CCCGCAACCC GCGCCGCAAG 
CGCAGTCTGA AGCGGCACGC CCAGGCCCAG CGGTCGCCCT CGACGACCCT GCCACCGATC 
ACGTGCTTCG ACCTCTCCCG CGACTCGCAG CTGATGGCCA TCGCCTCAGG CCGCAGCGTG 
CACCTGATGC GCATCAACAC GCCGGAGTAC CAGTGCAGCC TGGAGGGGCA CGCGGGCGTG 
GTCAACTGCC TGAAGTTCGC GCCCAACGGA GAGTTCCTCA CCACGGGATC CGACGATCGG 
CTCGTCCAGG TGTGGAACCT GGCGGCCACC GAGACCATTA ACACCTTCAA GGGGCATGCG 
GCGCCGGTGA TCAAGGTGGT GGTGCTGATG GACTCGCTGC GGGTGATCTC CACGGACCGG 
GACTCACTGC TGCTCGTGTG GATGGCCAGC TCGGGCAACC TGCTGCAGAC CATCCAGGGA 
CCCTACAAGA GCCTGGTGGT GACCAACAAC ATGCGCTTCG CCGCCTCCAG CAACGGGGAC 
AACACTCTGA AGATCTGGTC CCTCACCCAG GAGGACGAGA AGTACTCGGT CTCGCACTCG 
GACGAGATCA CCTGCTTCGA GATCAGCGCC GACAGCATGC ACATCATCAC GGGCTCCCGG 
GACATGTCGC TGAAGGTGTG GCAGTCCACG GGCGGCAAGC TCAGCCAGGT GCTGGTCGGG 
CACAGCGACG CTGTGACCTG TGTGGCCGTC TCGGTGACCA ACAAGACGCA GGTCCTCTCC 
GGCTCCAAGG ACACGAACCT CATCCTGTGG GACCTGCTCA CTGGCGAGGA GGTTCACACG 
CTGGCCGGAC ACTTGGGTCC AGTGATCGGA GTGAAGGTTT CGGCAGATGG CTCCACGGCT 
GTGTCGGGCA GCGATGACAA GACCTTGATC GTGTGGGAGA CGAAGCGCGG ATTGGCCCTG 
ACCTCGCTGC AGATGCACGT GCCCTTCACC CACTTCGACA TCAGCCTGGA GGTGTCGCGG 
GTCCTGGTCC AACTGGTGGA CAGCTACAAC CTCCCGGTGA TCTGTCTGCA CAACACCCCG 
GCGCAGTATG TAAAGCTGCC CACGTACTCG GGACCCACCA AGGATGTGGA TGATCTCCGG 
CCACAGGGCC CGAAGCGCCA GATGAAGCGC CTGCTGAAGA AGGAGGTCTC CCTGGACACG 
TACACTTGGC AGAAGAAGTA CGGCCACCTG ACCTCGTCGG TCATGATGGC CCAGGTTGAC 
GAGCGCCTGA AGCGACGCTT CAGCGTCTCG GCCAGCATGG AGGAGATCTC GAAGATCGCC 
GAGAACAAGA CAGGGGCCTC CAATGCCAAC ATCGGCCCCG AGCAGGCCGC TCTGGCCCAG 
TCGCAGCACT TCGACCAGCT GGAGGCGCTG TGGAACAAGC GGTCTCCGCC CCGACGCCGC 
CACAACGCGA TCACTTCGCT TCAGGAGGAG GAGGATACCA CAAGGGATAA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015040225.1
CDS	550..5586
Translation	MAAPIDESVLNALKGVTSKQTRLPKPLLIKIYVASQKQEFNQERRMLLELVGPELQGLYDDRQIELEFVDMHFGTGALEVNQLERDPYLLQDYLHEIDTCHAHTKSVFFLALIGDGLGRLPLPTRLDEDIYAAVLADPQTTADQEALLARWYEMDPSKVQRQLKQDYRVLPMDEWLSESQQMQKMLEQALQGFVQGPGASARSADFLKRVQQLRRTQIEREVEQAMALSSEKILAVFRERAAQCSKRDVEANERLRKIKDELTMNLSTDNHTTLVVPGSASCEMIDPDNEDHESYLSKFKNKVTDKLRLLIEAHITNDPDVIKGRKKTVQEIFHEHATHLRILRDHVEGAALIESRVPPQLRQNLMANFRNGSRHAPYFICGSDGSGKSALISTLYGQVASWFVSTKVHRVIRFAKATPRSAYNLELLRVICQQISIIFNIPEGYLPKDASFDPLYISTWFQNLLRRVEDMSNDVLFLFIDDLHLLNPLDCDIVTALSWLPTSLPWNVQIICSSTTPVEQLKFTPMQRDRFKSAEYQFDLNLGEYATELRRPPQCVSDVSFVLYVEQQFDGLERHYGRQAVGSLAAYITCSEYGLSETELLELLMPTDDPESLIDTRSGHFSFATFKKIHREMDKLLLLHDKIMSGKVLIQWRHNYCAVVAKRRYMGGQRTRNLHMELANLFFPQDEDESTLENESTQSENKSVISLKDTLRTVCDLPHKDREREKDNVSAGRKSSSTHHNDDTSTFYNPIAADVSYSMRHVEESWHHLMRSDDTTRFKQIAVCNFDFLLAAVQTVSISYLRCLIEHVRCYILDRDIELIYYTIRKSSDVLTRDPMQLGSQLISWLRPISEHDDDDNSLLSMTVRSATAWCDGYAVPLLVPLTGWLPAPLPSQIRTMTVSGTGVIRAVCLAPSRQHLILATSSGEVQQWHIMSNSLEHIFKGHTAAVTCLLVAPTSEPELLLTGSEDTSVLVWHVSQRDRRAHINAHTAPITGVAAGVNNTLIISSSEDATIAITDLASGKLKHRISHHRGPVTGILVAGACDVLISGSHDRTICVWNLENFALLNTMQMLSPVLRIDISWNSVFLLALCEDNALYVRTLATGKELHTLKGHKSKIRTISIGKDSQRCVVGCDDTRALIYDMHSGKLVRSMPPNPGPVTAVYAMENDDFLVTVGGNKITFYSFRNEELYVNPYSRNPRRKRSLKRHAQAQRSPSTTLPPITCFDLSRDSQLMAIASGRSVHLMRINTPEYQCSLEGHAGVVNCLKFAPNGEFLTTGSDDRLVQVWNLAATETINTFKGHAAPVIKVVVLMDSLRVISTDRDSLLLVWMASSGNLLQTIQGPYKSLVVTNNMRFAASSNGDNTLKIWSLTQEDEKYSVSHSDEITCFEISADSMHIITGSRDMSLKVWQSTGGKLSQVLVGHSDAVTCVAVSVTNKTQVLSGSKDTNLILWDLLTGEEVHTLAGHLGPVIGVKVSADGSTAVSGSDDKTLIVWETKRGLALTSLQMHVPFTHFDISLEVSRVLVQLVDSYNLPVICLHNTPAQYVKLPTYSGPTKDVDDLRPQGPKRQMKRLLKKEVSLDTYTWQKKYGHLTSSVMMAQVDERLKRRFSVSASMEEISKIAENKTGASNANIGPEQAALAQSQHFDQLEALWNKRSPPRRRHNAITSLQEEEDTTRDKA

Target ORF information:

RefSeq Version	XM_015184739.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA15661), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015184739.1

ORF Insert Sequence:

ATGGCCGCGC CCATTGACGA GAGTGTGCTG AACGCACTGA AGGGGGTGAC CTCCAAGCAG 
ACCCGCCTGC CCAAGCCGCT GCTCATCAAG ATCTATGTCG CCAGCCAGAA GCAGGAGTTC 
AACCAGGAGC GCCGCATGCT CCTGGAGCTG GTGGGCCCCG AGCTGCAGGG CCTCTACGAC 
GACCGCCAAA TCGAGTTGGA GTTCGTGGAC ATGCACTTCG GCACTGGGGC GCTGGAGGTG 
AACCAGCTGG AGCGGGATCC CTACCTGCTG CAGGACTACC TGCACGAGAT CGACACCTGT 
CATGCCCACA CGAAGAGCGT CTTCTTTCTG GCGCTCATCG GCGACGGCCT CGGACGCCTG 
CCGCTGCCCA CGCGCCTGGA CGAGGACATC TATGCCGCGG TGCTGGCCGA CCCTCAGACA 
ACGGCCGACC AGGAGGCTCT TCTAGCCAGG TGGTACGAGA TGGATCCATC CAAGGTGCAG 
CGTCAGCTGA AGCAGGACTA TCGAGTACTG CCCATGGACG AGTGGCTCAG CGAGTCCCAG 
CAGATGCAAA AGATGCTGGA GCAGGCGCTG CAAGGCTTTG TCCAAGGACC AGGTGCCTCG 
GCGCGATCTG CAGACTTCCT GAAGCGGGTG CAGCAGCTGA GGCGCACGCA GATCGAACGC 
GAAGTGGAGC AGGCCATGGC CCTCTCCAGC GAGAAGATCC TCGCCGTGTT TCGGGAACGG 
GCCGCCCAGT GCAGCAAGCG GGATGTGGAG GCCAATGAGC GGCTTCGGAA GATCAAGGAC 
GAGCTCACCA TGAACCTCTC CACGGACAAT CACACAACCC TAGTGGTGCC AGGCAGTGCC 
TCGTGCGAAA TGATCGACCC GGACAACGAG GACCACGAGT CCTATCTCAG CAAATTCAAG 
AACAAAGTCA CCGACAAGCT GCGTCTGCTA ATCGAGGCGC ACATCACCAA TGATCCCGAT 
GTAATCAAGG GGAGGAAAAA GACTGTTCAG GAAATCTTTC ACGAGCACGC CACTCACCTG 
CGCATCCTGC GCGATCATGT GGAGGGGGCG GCCCTCATCG AGTCGCGGGT GCCACCGCAG 
CTGCGCCAGA ACCTGATGGC GAACTTCAGG AACGGCAGCC GGCATGCGCC GTACTTCATT 
TGCGGCTCGG ATGGCAGCGG CAAGAGCGCC CTGATCAGCA CCCTGTACGG GCAGGTGGCC 
AGCTGGTTCG TCTCGACGAA GGTCCACCGC GTGATCCGCT TCGCGAAGGC CACGCCGCGC 
TCGGCCTACA ACCTGGAGCT GCTGCGCGTG ATCTGCCAGC AGATATCCAT CATCTTCAAC 
ATCCCCGAGG GCTACCTACC CAAGGACGCC TCCTTCGATC CGCTGTACAT CAGCACCTGG 
TTCCAGAACC TGCTGCGGCG CGTCGAGGAC ATGAGCAACG ACGTGCTGTT CCTGTTCATC 
GACGACCTGC ACCTGCTGAA CCCCCTGGAC TGCGACATCG TGACCGCCCT CTCCTGGCTG 
CCCACCTCGC TGCCCTGGAA CGTGCAGATC ATCTGCAGCT CCACCACCCC CGTCGAGCAG 
CTCAAGTTCA CGCCCATGCA GCGGGACCGC TTCAAGTCGG CGGAGTACCA GTTCGACCTC 
AACCTGGGCG AGTACGCCAC GGAGCTGCGG CGACCTCCGC AGTGCGTCTC GGACGTCAGC 
TTCGTCCTGT ACGTGGAGCA GCAGTTCGAC GGCCTGGAGC GCCACTACGG CAGGCAGGCG 
GTGGGCAGCC TGGCCGCCTA CATCACCTGC TCCGAGTACG GGCTGAGCGA GACGGAGCTC 
CTGGAGCTGC TCATGCCCAC CGACGATCCC GAGTCCCTGA TCGACACCAG AAGCGGACAC 
TTTAGCTTCG CCACCTTCAA GAAGATCCAC AGGGAAATGG ACAAGCTCCT GCTGCTGCAC 
GACAAGATCA TGTCCGGCAA GGTGCTCATC CAGTGGCGCC ACAACTACTG CGCCGTGGTG 
GCCAAGCGAC GGTACATGGG CGGCCAGAGG ACGCGCAACC TGCACATGGA GCTGGCCAAC 
CTGTTCTTCC CGCAGGACGA GGACGAGAGC ACGCTGGAGA ACGAGTCCAC CCAGAGCGAG 
AACAAGTCCG TGATTAGCCT CAAGGACACG CTCAGGACAG TCTGCGACCT GCCGCACAAG 
GACAGGGAGC GCGAGAAGGA CAACGTCTCC GCTGGCCGCA AGTCCTCCTC CACCCACCAC 
AACGACGACA CCTCCACCTT CTACAACCCC ATCGCCGCCG ACGTCAGCTA CAGCATGCGG 
CACGTGGAGG AGAGCTGGCA CCACCTGATG CGCTCCGACG ACACGACACG CTTCAAGCAA 
ATCGCAGTCT GCAACTTTGA TTTCCTGCTG GCGGCGGTAC AAACTGTGTC GATCAGCTAT 
TTGCGCTGCC TGATCGAGCA CGTCCGCTGC TATATCCTCG ACAGGGACAT CGAGCTGATC 
TACTACACGA TCCGCAAGTC CAGCGACGTG CTCACGCGCG ACCCCATGCA GCTGGGCTCC 
CAGCTGATCT CCTGGCTGCG TCCGATCAGC GAGCACGACG ACGACGACAA CTCGCTGCTC 
AGCATGACGG TGCGCTCGGC GACGGCCTGG TGCGATGGCT ACGCCGTGCC GCTGCTGGTG 
CCGCTCACGG GATGGCTGCC GGCACCGCTG CCCTCGCAGA TCCGCACCAT GACGGTGTCG 
GGCACGGGCG TCATCCGAGC CGTCTGCCTG GCGCCCTCGA GGCAGCACCT CATCCTGGCC 
ACCAGCTCGG GCGAGGTGCA GCAGTGGCAC ATCATGAGCA ACTCGCTGGA ACACATCTTC 
AAGGGACACA CTGCGGCAGT CACGTGCCTG CTGGTGGCTC CGACCAGCGA GCCGGAGCTC 
CTCCTCACCG GCTCCGAGGA CACCAGCGTG CTCGTCTGGC ACGTCTCGCA GCGCGACCGT 
CGGGCGCACA TCAACGCCCA CACAGCCCCC ATAACGGGCG TGGCGGCCGG GGTCAATAAC 
ACGCTGATCA TCAGCAGCAG CGAGGATGCC ACAATCGCCA TCACGGACCT GGCCAGCGGC 
AAACTGAAAC ACCGAATCAG CCACCATCGC GGCCCTGTGA CCGGGATCCT GGTGGCCGGA 
GCGTGCGATG TCCTGATCTC GGGCAGCCAC GACCGAACCA TCTGCGTGTG GAACCTCGAG 
AACTTTGCGC TGCTGAACAC CATGCAAATG CTGAGCCCCG TCCTGCGCAT CGACATCTCA 
TGGAACTCGG TATTCCTCCT GGCCCTGTGC GAGGACAATG CCCTCTATGT GCGCACTCTG 
GCCACGGGCA AGGAGCTGCA CACGCTCAAG GGCCACAAGT CAAAGATACG GACCATTTCC 
ATTGGCAAGG ACAGTCAGCG CTGCGTGGTG GGATGCGACG ATACGCGAGC CCTGATCTAC 
GACATGCACT CCGGCAAGCT GGTGCGCTCC ATGCCGCCCA ACCCGGGGCC GGTGACCGCC 
GTCTATGCGA TGGAGAACGA CGACTTCCTG GTCACGGTGG GCGGCAACAA GATCACGTTC 
TACTCGTTCC GCAACGAGGA GCTCTACGTG AACCCGTACT CCCGCAACCC GCGCCGCAAG 
CGCAGTCTGA AGCGGCACGC CCAGGCCCAG CGGTCGCCCT CGACGACCCT GCCACCGATC 
ACGTGCTTCG ACCTCTCCCG CGACTCGCAG CTGATGGCCA TCGCCTCAGG CCGCAGCGTG 
CACCTGATGC GCATCAACAC GCCGGAGTAC CAGTGCAGCC TGGAGGGGCA CGCGGGCGTG 
GTCAACTGCC TGAAGTTCGC GCCCAACGGA GAGTTCCTCA CCACGGGATC CGACGATCGG 
CTCGTCCAGG TGTGGAACCT GGCGGCCACC GAGACCATTA ACACCTTCAA GGGGCATGCG 
GCGCCGGTGA TCAAGGTGGT GGTGCTGATG GACTCGCTGC GGGTGATCTC CACGGACCGG 
GACTCACTGC TGCTCGTGTG GATGGCCAGC TCGGGCAACC TGCTGCAGAC CATCCAGGGA 
CCCTACAAGA GCCTGGTGGT GACCAACAAC ATGCGCTTCG CCGCCTCCAG CAACGGGGAC 
AACACTCTGA AGATCTGGTC CCTCACCCAG GAGGACGAGA AGTACTCGGT CTCGCACTCG 
GACGAGATCA CCTGCTTCGA GATCAGCGCC GACAGCATGC ACATCATCAC GGGCTCCCGG 
GACATGTCGC TGAAGGTGTG GCAGTCCACG GGCGGCAAGC TCAGCCAGGT GCTGGTCGGG 
CACAGCGACG CTGTGACCTG TGTGGCCGTC TCGGTGACCA ACAAGACGCA GGTCCTCTCC 
GGCTCCAAGG ACACGAACCT CATCCTGTGG GACCTGCTCA CTGGCGAGGA GGTTCACACG 
CTGGCCGGAC ACTTGGGTCC AGTGATCGGA GTGAAGGTTT CGGCAGATGG CTCCACGGCT 
GTGTCGGGCA GCGATGACAA GACCTTGATC GTGTGGGAGA CGAAGCGCGG ATTGGCCCTG 
ACCTCGCTGC AGATGCACGT GCCCTTCACC CACTTCGACA TCAGCCTGGA GGTGTCGCGG 
GTCCTGGTCC AACTGGTGGA CAGCTACAAC CTCCCGGTGA TCTGTCTGCA CAACACCCCG 
GCGCAGTATG TAAAGCTGCC CACGTACTCG GGACCCACCA AGGATGTGGA TGATCTCCGG 
CCACAGGGCC CGAAGCGCCA GATGAAGCGC CTGCTGAAGA AGGAGGTCTC CCTGGACACG 
TACACTTGGC AGAAGAAGTA CGGCCACCTG ACCTCGTCGG TCATGATGGC CCAGGTTGAC 
GAGCGCCTGA AGCGACGCTT CAGCGTCTCG GCCAGCATGG AGGAGATCTC GAAGATCGCC 
GAGAACAAGA CAGGGGCCTC CAATGCCAAC ATCGGCCCCG AGCAGGCCGC TCTGGCCCAG 
TCGCAGCACT TCGACCAGCT GGAGGCGCTG TGGAACAAGC GGTCTCCGCC CCGACGCCGC 
CACAACGCGA TCACTTCGCT TCAGGAGGAG GAGGATACCA CAAGGGATAA GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA15661 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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