Dpse\GA14479 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA14479
Entrez Gene ID	4802835
Full Name	GA14479 gene product from transcript GA14479-RB
Synonyms	GA14479,dpse_GLEANR_6238
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001359634.4	XP_001359671.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA14479 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA14479 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe02286	XM_001359634.4 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA14479), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA14479 ( XM_001359634.4 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001359671.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA14479), mRNA.

Price & Availability↑

CloneID	ODe02286
Clone ID Related Accession (Same CDS sequence)	XM_001359634.4
Accession Version	XM_001359634.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001359634.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGATT CACCCAAAGC CAACAACAAC ATGAATAAAG TGTCTCCCGT TGACAAGATG 
AGCTCCAGCA TCAACAGCAC CCTGGAGGAC ACAACACCCA GTGATTCAGG GGTGCAGTTG 
CTCGACTCCG AGAACAGCGA TGTGATGCTG GAGTCCATGT GCTCCTATGA GGAGAACTCG 
AGACCGCTGA AAGTCCCTTC CCCCCAGCTA CCTTCCATAG ACCCCAACTC CAATGTGGCA 
CCGGCTGAGT CCATGATAAC TAGTGATTGT TCGACCTTTG ACACCACTGA GAAAATTGTG 
TACAGGCGAA AGGTGCACAA GACTTCGTCC GCATCAAAAG CCCCGAAAAA GCGAGTGTCC 
TTTCACGAGG ATATTCTGAA GAACACTCGC ACCGACAACA TTCACATAGA GCATGGCTTT 
ATAACGTACA AAAACGGCCG CAAGCTGGCC TTTGCAAACT CGGCGGGTGT GGCAGGTGCT 
CCCGGTCGGT ATTCCTGGTG CCCGGAGCGA GAGCGTTTCG AGGGACCCAA TGAGATCTCT 
GCAGCTGGCA GAGATTATGA TGATGGCGAT GGTGAAGATG CAATGTCCGG CGGTGGTGGA 
GGTGGTCGCA GACGACATTT GGTGTACAGA AATGCCTGTT CGGATGTGCT GGACTACGTG 
AACACGGACA TGTTCGATAC GAATGAGCCC GTTGGCCTGC AGTACGACTC ATCGGGTGTG 
TTCGAATATA CCCCGTCTGG GCAGAAACTG CTGCAGCAGG CAGCGCCTGT CCTATACAAT 
TGCGTTTGCA GCAGCTCCAA CTCCAGCCTG GATTCGGACG AACAGGACAA GAACTCCAAT 
GCCAATCGAA AACAATACGA GCAGGCGAAG AGCAGCTCCT GCGATTGCAT CGGCATGAGT 
AATGCAAACA ATAATTTGAT TGGTGACAAC TGCTACTACT CGGAGCCCAA CATTGGCGAT 
GCCGATGGTG GTCCCACAAT GAAATCAGTT TGGAACAAGG AGAAAAAGCC AAAGTCCAGC 
TGCCTTAAGA AAACCAAACG TCAGACAAAC ATTATTCTGG AACAAGATCT CAATGGCCGC 
GTCAAGACCT TTAATGTGCA CGACATTAAG CATCAACTAA TCGACAACAG CAGCAAAATG 
ATATTCGGCT CCTTGAAAAG TATATTCACG ATGCCCTTGC CGGAACGCGG TGTGCCCGAG 
GGGTCAGAGG ACCTACAGGC GGTCGTTGAG TGCGTCCCAG AACTGGAGAG CACGCTGGAA 
GCCAAAACAA ATCGTGCAAT CGTGGAGTCG CCCACGCTCA AAGCCAAGCC ATTCCTGAGC 
AAAAGTCTCG ATGGCAATAA GCAGACACAG TCGGTTAAGA AATTTGTGCA CAACGTAGAT 
GAGCAGCTTC GGCATAAGAA TGATGATAGC TCCATCGAAG CCTCGCCCAC AGAGACAAGA 
CCATCGCACA CTTTACAACG TCAGGAGGAG TTCGATAAGG AAACAGTCAT AGCAGCCATA 
AGCAGCATTC CGAATGAATT CCGCAGCAAA TTTATTATCA ACTGCGAAAG CACTGTCTTC 
GAGCACACTG GCGTGTCCTA CGAGACTGGG GCTGGGCAGC CGCAGCCAGA CTTCTCCAAC 
ACAGCTCCAC GCCACTCCAG CGTAGCCGGT TTGCTGGAGC AAAACACACC CATAGCCGAG 
CCAGCAGCTA ATCAGATCAA GAAGTCGTTC ATGGATGCAC CCATAGCTAA GACGTTTAGT 
AATTTCTTTC GCAGCCTGAA GGAAAGCACA AGTGCTGTCG GAACTCCAGT TGAAAGACCA 
GAGGAAAGAG CAGCCCCAGG CCCAGCCCCA GCCCCAGCTC CAACTCCAGA TGATTGTACT 
ACTCTAAAAA TGTCTAAGCC ACAACAACAA GCGCCCTCCA GCTGTAAGAT GTACAGAAAT 
CACTCTTCAT CCACCATATC GGAACCTACA AGTTCCACCA ACAACAGCAG CAGCCACCAC 
CAAATATCCA ACCAGCAACC GAATAATACA GATCGCCATT CACGTCATCT GGCCTCTCCT 
CTAAAGAAAC ATGGCCAGCC AATGCTGCCG ACTCGGTACT ACGACCAGCA GCAGCTCCAC 
AGTCGTAGCC TTCACAGCCC CAACAGCTAT CTGGCAGCTC GCGGCGGCCC TGGCGGAAGT 
GGACGCGACT CAAAAAGCAC CATTCTGAGC GAGGAATACG ATGATATACT TACCATAACC 
ACCACAGATA CGACAAACGA CAAGCGTTCC ATGGACAGTG ATCTGGTGAT CGTTGACTAC 
ACCGATGTGG ACTATGCTCA TCTTCTGAAA CCTCCAGCCC CCGCAGCAAA GACCTCATTA 
ATCAATCGAT TCCTACGAAA TGTCACCCAG AAGAAAATAC TAGAAACCTC CATACGAAAG 
AACAATTTCT TTGCGGATAA ACTGCGGAGC GAACAGAGGC TTTATGTCAA GGGGGCGCGA 
CCCAAGAACT ATGAGCTAAT AGACGACCTA AATGCGGAGA TCGCAATGGA GATTGAAATG 
TGTGGAACGA ATTCCCCGCG CCGTGAGCTG GCTGTGAGCC TGGACACGGA TACGCCAGAG 
AGTGTATCCA GCTTTGAGCT TGGCATCGGG GAAATATCGA TTGACTTGTT CCATGGAAGA 
AGTCTGCTTA ATTTAAAAGA TTCCAATGAG CAGCTTTTGA AGGTATTCAA GCTCTATACG 
GGCTACAGCA GCGAGGGTGT TATGACCCCA GTGTTGGTTT TGCTGACGGA CAGGACACTG 
TATGTGGCAG ATCTGGCAGA CCAGGTTCGT CTCTGTGCCA AGTTCGTTTT GGCCTACAAA 
GACTTGGATG TCATTTTGAT GGGTCCCTTC GGCAATACGG TTCTACTGAG TAACAGCATG 
CGGGACATGC AGCAGGTTTT ACTGGCAGGT GGACCATATC CAGCAGGATC CCTTGTTGCC 
AATTTGGAGC TTTGTGCTAG ACGCAGCGGC GCCACTCTAC CGGCTGTGGG TCAACTGACA 
CTGGAGCATT TGGCCCCGCT GCAGGCTTTT GTTCGGGCCA ACAGCTGTGT GGGCAACGAC 
GAACACTGGC TATTCTATGC CGTTGTCAAT GTGCCAGCTG GCAATGCACT AAGAGCAGAT 
TCACCTGGTG AGCCACTGGG GCCATCTATT AAGGGATTCC TCATGCACCG CCGGTTTAAG 
GAAACAGCAA CCACGACAGC AGCGAGCAGG GTCTACTGGC AGGCAGGATA CTTTTCATTG 
AAAGCCGGCG TCTTGTATAT GTTCAACGAC TCCACACAGA CGCGTCCCAG TTGGGCAATG 
GCTCTTGCCG AGTGTCAAGG CGCCCGACGG ACTCTCAAGT CCGAGCGCCC CCATTGCTTT 
GAGATTATGT TAAAGGGTGA ACTCCTCCAA ATGGCCGCAC CGGATGAGTA TGTGGCATCC 
GAATGGTTGC AAGCCCTTAT ACAGTCGGCC ACTGGCCATT TGACACCGCA AGAGAAACAC 
AAAACCTTGG GCTGCACTCT AATCGTTACG GAAAATCACT TGATAACCTT GCGTGAAGAT 
TTCTCGGCAC CCTTGAGGCC ACTTAGCCAT CAGGTGGATA CAAACGTGGT TCCACAAACC 
AAACAACTCT TATATGAAGA AGAGCTGTTG AGTGTTTTTG AAACTAGTAG CCTGATGGGC 
TCAACAATGA GTACACCGAC TCGGGGGACT CAGCGGTCTT TCTCTTCTCT GAGCTCCACG 
CCTACAAAGA ATGGAAACGA TCCGGACATG TCTAAGGAAA ATACGTCCTC TCCGGGAAAA 
TCTCAACGAA TGAGCAGTGT ATATGGAAAG AACTCTGGTC TGGCGATACT CACGTGTGGG 
GACATCAAAG CCATGACTGG CATTAAGATA ACCTCGCGTG CGGAGACCTG GTGGTGCATA 
CTGGAGTTTT CGTGCCAGGA GGCGCCCTTG GAGAGTCTCG ATGATTTGGT TATCTTTTTT 
GCCAGCAGCA TGGAAATGCA ACGATTTTTG CGATTACTGG AACAGCTTTG GCAGGCAAAG 
AATAACGATC TCTTTCCAAT AACAATACTG GACGATGGTG AAGATTTAGC CGAGCAGTGC 
ACCCTTCTCT ATATAGACAC CAATCGAGCA TGGGAGCCCT TGCTATCGGC GGCAATGGGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001359671.3
CDS	294..4436
Translation	MMDSPKANNNMNKVSPVDKMSSSINSTLEDTTPSDSGVQLLDSENSDVMLESMCSYEENSRPLKVPSPQLPSIDPNSNVAPAESMITSDCSTFDTTEKIVYRRKVHKTSSASKAPKKRVSFHEDILKNTRTDNIHIEHGFITYKNGRKLAFANSAGVAGAPGRYSWCPERERFEGPNEISAAGRDYDDGDGEDAMSGGGGGGRRRHLVYRNACSDVLDYVNTDMFDTNEPVGLQYDSSGVFEYTPSGQKLLQQAAPVLYNCVCSSSNSSLDSDEQDKNSNANRKQYEQAKSSSCDCIGMSNANNNLIGDNCYYSEPNIGDADGGPTMKSVWNKEKKPKSSCLKKTKRQTNIILEQDLNGRVKTFNVHDIKHQLIDNSSKMIFGSLKSIFTMPLPERGVPEGSEDLQAVVECVPELESTLEAKTNRAIVESPTLKAKPFLSKSLDGNKQTQSVKKFVHNVDEQLRHKNDDSSIEASPTETRPSHTLQRQEEFDKETVIAAISSIPNEFRSKFIINCESTVFEHTGVSYETGAGQPQPDFSNTAPRHSSVAGLLEQNTPIAEPAANQIKKSFMDAPIAKTFSNFFRSLKESTSAVGTPVERPEERAAPGPAPAPAPTPDDCTTLKMSKPQQQAPSSCKMYRNHSSSTISEPTSSTNNSSSHHQISNQQPNNTDRHSRHLASPLKKHGQPMLPTRYYDQQQLHSRSLHSPNSYLAARGGPGGSGRDSKSTILSEEYDDILTITTTDTTNDKRSMDSDLVIVDYTDVDYAHLLKPPAPAAKTSLINRFLRNVTQKKILETSIRKNNFFADKLRSEQRLYVKGARPKNYELIDDLNAEIAMEIEMCGTNSPRRELAVSLDTDTPESVSSFELGIGEISIDLFHGRSLLNLKDSNEQLLKVFKLYTGYSSEGVMTPVLVLLTDRTLYVADLADQVRLCAKFVLAYKDLDVILMGPFGNTVLLSNSMRDMQQVLLAGGPYPAGSLVANLELCARRSGATLPAVGQLTLEHLAPLQAFVRANSCVGNDEHWLFYAVVNVPAGNALRADSPGEPLGPSIKGFLMHRRFKETATTTAASRVYWQAGYFSLKAGVLYMFNDSTQTRPSWAMALAECQGARRTLKSERPHCFEIMLKGELLQMAAPDEYVASEWLQALIQSATGHLTPQEKHKTLGCTLIVTENHLITLREDFSAPLRPLSHQVDTNVVPQTKQLLYEEELLSVFETSSLMGSTMSTPTRGTQRSFSSLSSTPTKNGNDPDMSKENTSSPGKSQRMSSVYGKNSGLAILTCGDIKAMTGIKITSRAETWWCILEFSCQEAPLESLDDLVIFFASSMEMQRFLRLLEQLWQAKNNDLFPITILDDGEDLAEQCTLLYIDTNRAWEPLLSAAMG

Target ORF information:

RefSeq Version	XM_001359634.4
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA14479), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001359634.4

ORF Insert Sequence:

ATGATGGATT CACCCAAAGC CAACAACAAC ATGAATAAAG TGTCTCCCGT TGACAAGATG 
AGCTCCAGCA TCAACAGCAC CCTGGAGGAC ACAACACCCA GTGATTCAGG GGTGCAGTTG 
CTCGACTCCG AGAACAGCGA TGTGATGCTG GAGTCCATGT GCTCCTATGA GGAGAACTCG 
AGACCGCTGA AAGTCCCTTC CCCCCAGCTA CCTTCCATAG ACCCCAACTC CAATGTGGCA 
CCGGCTGAGT CCATGATAAC TAGTGATTGT TCGACCTTTG ACACCACTGA GAAAATTGTG 
TACAGGCGAA AGGTGCACAA GACTTCGTCC GCATCAAAAG CCCCGAAAAA GCGAGTGTCC 
TTTCACGAGG ATATTCTGAA GAACACTCGC ACCGACAACA TTCACATAGA GCATGGCTTT 
ATAACGTACA AAAACGGCCG CAAGCTGGCC TTTGCAAACT CGGCGGGTGT GGCAGGTGCT 
CCCGGTCGGT ATTCCTGGTG CCCGGAGCGA GAGCGTTTCG AGGGACCCAA TGAGATCTCT 
GCAGCTGGCA GAGATTATGA TGATGGCGAT GGTGAAGATG CAATGTCCGG CGGTGGTGGA 
GGTGGTCGCA GACGACATTT GGTGTACAGA AATGCCTGTT CGGATGTGCT GGACTACGTG 
AACACGGACA TGTTCGATAC GAATGAGCCC GTTGGCCTGC AGTACGACTC ATCGGGTGTG 
TTCGAATATA CCCCGTCTGG GCAGAAACTG CTGCAGCAGG CAGCGCCTGT CCTATACAAT 
TGCGTTTGCA GCAGCTCCAA CTCCAGCCTG GATTCGGACG AACAGGACAA GAACTCCAAT 
GCCAATCGAA AACAATACGA GCAGGCGAAG AGCAGCTCCT GCGATTGCAT CGGCATGAGT 
AATGCAAACA ATAATTTGAT TGGTGACAAC TGCTACTACT CGGAGCCCAA CATTGGCGAT 
GCCGATGGTG GTCCCACAAT GAAATCAGTT TGGAACAAGG AGAAAAAGCC AAAGTCCAGC 
TGCCTTAAGA AAACCAAACG TCAGACAAAC ATTATTCTGG AACAAGATCT CAATGGCCGC 
GTCAAGACCT TTAATGTGCA CGACATTAAG CATCAACTAA TCGACAACAG CAGCAAAATG 
ATATTCGGCT CCTTGAAAAG TATATTCACG ATGCCCTTGC CGGAACGCGG TGTGCCCGAG 
GGGTCAGAGG ACCTACAGGC GGTCGTTGAG TGCGTCCCAG AACTGGAGAG CACGCTGGAA 
GCCAAAACAA ATCGTGCAAT CGTGGAGTCG CCCACGCTCA AAGCCAAGCC ATTCCTGAGC 
AAAAGTCTCG ATGGCAATAA GCAGACACAG TCGGTTAAGA AATTTGTGCA CAACGTAGAT 
GAGCAGCTTC GGCATAAGAA TGATGATAGC TCCATCGAAG CCTCGCCCAC AGAGACAAGA 
CCATCGCACA CTTTACAACG TCAGGAGGAG TTCGATAAGG AAACAGTCAT AGCAGCCATA 
AGCAGCATTC CGAATGAATT CCGCAGCAAA TTTATTATCA ACTGCGAAAG CACTGTCTTC 
GAGCACACTG GCGTGTCCTA CGAGACTGGG GCTGGGCAGC CGCAGCCAGA CTTCTCCAAC 
ACAGCTCCAC GCCACTCCAG CGTAGCCGGT TTGCTGGAGC AAAACACACC CATAGCCGAG 
CCAGCAGCTA ATCAGATCAA GAAGTCGTTC ATGGATGCAC CCATAGCTAA GACGTTTAGT 
AATTTCTTTC GCAGCCTGAA GGAAAGCACA AGTGCTGTCG GAACTCCAGT TGAAAGACCA 
GAGGAAAGAG CAGCCCCAGG CCCAGCCCCA GCCCCAGCTC CAACTCCAGA TGATTGTACT 
ACTCTAAAAA TGTCTAAGCC ACAACAACAA GCGCCCTCCA GCTGTAAGAT GTACAGAAAT 
CACTCTTCAT CCACCATATC GGAACCTACA AGTTCCACCA ACAACAGCAG CAGCCACCAC 
CAAATATCCA ACCAGCAACC GAATAATACA GATCGCCATT CACGTCATCT GGCCTCTCCT 
CTAAAGAAAC ATGGCCAGCC AATGCTGCCG ACTCGGTACT ACGACCAGCA GCAGCTCCAC 
AGTCGTAGCC TTCACAGCCC CAACAGCTAT CTGGCAGCTC GCGGCGGCCC TGGCGGAAGT 
GGACGCGACT CAAAAAGCAC CATTCTGAGC GAGGAATACG ATGATATACT TACCATAACC 
ACCACAGATA CGACAAACGA CAAGCGTTCC ATGGACAGTG ATCTGGTGAT CGTTGACTAC 
ACCGATGTGG ACTATGCTCA TCTTCTGAAA CCTCCAGCCC CCGCAGCAAA GACCTCATTA 
ATCAATCGAT TCCTACGAAA TGTCACCCAG AAGAAAATAC TAGAAACCTC CATACGAAAG 
AACAATTTCT TTGCGGATAA ACTGCGGAGC GAACAGAGGC TTTATGTCAA GGGGGCGCGA 
CCCAAGAACT ATGAGCTAAT AGACGACCTA AATGCGGAGA TCGCAATGGA GATTGAAATG 
TGTGGAACGA ATTCCCCGCG CCGTGAGCTG GCTGTGAGCC TGGACACGGA TACGCCAGAG 
AGTGTATCCA GCTTTGAGCT TGGCATCGGG GAAATATCGA TTGACTTGTT CCATGGAAGA 
AGTCTGCTTA ATTTAAAAGA TTCCAATGAG CAGCTTTTGA AGGTATTCAA GCTCTATACG 
GGCTACAGCA GCGAGGGTGT TATGACCCCA GTGTTGGTTT TGCTGACGGA CAGGACACTG 
TATGTGGCAG ATCTGGCAGA CCAGGTTCGT CTCTGTGCCA AGTTCGTTTT GGCCTACAAA 
GACTTGGATG TCATTTTGAT GGGTCCCTTC GGCAATACGG TTCTACTGAG TAACAGCATG 
CGGGACATGC AGCAGGTTTT ACTGGCAGGT GGACCATATC CAGCAGGATC CCTTGTTGCC 
AATTTGGAGC TTTGTGCTAG ACGCAGCGGC GCCACTCTAC CGGCTGTGGG TCAACTGACA 
CTGGAGCATT TGGCCCCGCT GCAGGCTTTT GTTCGGGCCA ACAGCTGTGT GGGCAACGAC 
GAACACTGGC TATTCTATGC CGTTGTCAAT GTGCCAGCTG GCAATGCACT AAGAGCAGAT 
TCACCTGGTG AGCCACTGGG GCCATCTATT AAGGGATTCC TCATGCACCG CCGGTTTAAG 
GAAACAGCAA CCACGACAGC AGCGAGCAGG GTCTACTGGC AGGCAGGATA CTTTTCATTG 
AAAGCCGGCG TCTTGTATAT GTTCAACGAC TCCACACAGA CGCGTCCCAG TTGGGCAATG 
GCTCTTGCCG AGTGTCAAGG CGCCCGACGG ACTCTCAAGT CCGAGCGCCC CCATTGCTTT 
GAGATTATGT TAAAGGGTGA ACTCCTCCAA ATGGCCGCAC CGGATGAGTA TGTGGCATCC 
GAATGGTTGC AAGCCCTTAT ACAGTCGGCC ACTGGCCATT TGACACCGCA AGAGAAACAC 
AAAACCTTGG GCTGCACTCT AATCGTTACG GAAAATCACT TGATAACCTT GCGTGAAGAT 
TTCTCGGCAC CCTTGAGGCC ACTTAGCCAT CAGGTGGATA CAAACGTGGT TCCACAAACC 
AAACAACTCT TATATGAAGA AGAGCTGTTG AGTGTTTTTG AAACTAGTAG CCTGATGGGC 
TCAACAATGA GTACACCGAC TCGGGGGACT CAGCGGTCTT TCTCTTCTCT GAGCTCCACG 
CCTACAAAGA ATGGAAACGA TCCGGACATG TCTAAGGAAA ATACGTCCTC TCCGGGAAAA 
TCTCAACGAA TGAGCAGTGT ATATGGAAAG AACTCTGGTC TGGCGATACT CACGTGTGGG 
GACATCAAAG CCATGACTGG CATTAAGATA ACCTCGCGTG CGGAGACCTG GTGGTGCATA 
CTGGAGTTTT CGTGCCAGGA GGCGCCCTTG GAGAGTCTCG ATGATTTGGT TATCTTTTTT 
GCCAGCAGCA TGGAAATGCA ACGATTTTTG CGATTACTGG AACAGCTTTG GCAGGCAAAG 
AATAACGATC TCTTTCCAAT AACAATACTG GACGATGGTG AAGATTTAGC CGAGCAGTGC 
ACCCTTCTCT ATATAGACAC CAATCGAGCA TGGGAGCCCT TGCTATCGGC GGCAATGGGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA14479 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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