Dpse\GA26445 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA26445
Entrez Gene ID	4802105
Full Name	GA26445 gene product from transcript GA26445-RF
Synonyms	Dpse_GA18227,Dpse_GA18333,GA18227,GA18333,GA26445,dpse_GLEANR_3608
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_003736611.2	XP_003736659.2	uncharacterized protein, isoform F
XM_002137568.2	XP_002137604.2	uncharacterized protein, isoform A
XM_015182127.1	XP_015037613.1	uncharacterized protein, isoform D
XM_015182128.1	XP_015037614.1	uncharacterized protein, isoform E
XM_015182129.1	XP_015037615.1	uncharacterized protein, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA26445 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA26445 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe20429	XM_015182129.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA26445), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODe20427	XM_015182127.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA26445), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODe01657	XM_003736611.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA26445), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe19586	XM_002137568.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA26445), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe20428	XM_015182128.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA26445), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA26445 ( XM_015182129.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015037615.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA26445), mRNA.

Price & Availability↑

CloneID	ODe20429
Clone ID Related Accession (Same CDS sequence)	XM_015182129.1
Accession Version	XM_015182129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCGTGG TGCCCTCTGT ACGGTGCAGC AACGTCGCAG AGTGGATGGG AAGACGCGAT 
GCCTGGTCAG CGGAGAAACC CCCGAATACC AGAACGAAAC CTACCCGATG GGAGTTCCAT 
GACGGTCGTG AACATCCTAG CGAAAACGTA GGGAGATTTC AGGACACCCG AGAGAAACAT 
CATGTACAAT CAGCTGAGGA GCAACAGGGA CGCATTAGAA AACAACGTAC AGCCAAAGAC 
GATCTTCATC TTAGTTTTCA TAACTCTAGA ACTAATCTTA TTAATGACCG CGACGTATAC 
ATGGAAGACT TAGTTGAAAA TCGAGATATA GTGGATGACA TTACCCGAAA CCGAGAAATT 
AAACAATTGG ACTATGCGCA AAGACAGCGC TGTGAAACCG AAGCCCACCA AATCGACGAC 
GACTCTATGC GTAGACACGA AATAGATCGC GGTAGCGACA TGGACTTCCC CGCTGCCCAA 
AACAGTATGA AGAGCAAACG CGAACTGTTT ATAAAAGAAG GGAACGTGGA AATTCTTCAA 
CTGATGACCA GAGATAAAAT TCACGATGGT GCTGCACTAG ACGATGACAA CATATATGTA 
AACGTGCCAC TAAAGCCCTT AGGAAACCTG TCACATCCGC AGCTTCTAAT GGTGGACAAT 
ACGGGTAAAG AGATTCTTAT GCGGAGATTT ATTGAAGAGC AACCAGATGG CAAGCAGATA 
ATTCGAGAGC ACTATCAAAT AGTACCAGGA GCAACCTACA TTCAATCCAT GCCGAATGAA 
GTACAACAAG CCTCAAATTT AAAAGTCGAT ACGTATCCAT TAGTCAAATC AGGGCCCAAC 
AGTATTGTTT ATTCTCAAAT GGAACCGGAA GTAAAGGTCA TACATACACA GCCAGAGCAT 
GCTGTTGAAG CTAGTTCAAA GATGCGGCCT GCAATATCGA ACCACTCTCT TACCCAGGAG 
CTGGAGCAGT CTCTTAAGCA ACAGAATTCC CTGCTGCGAC AGATACTGCT CGAAAAGGAC 
AAATTGGAGT CCGCCTATAC ACAGGAAGTG GCATTAGAAA CACAGAGTCT GCCTGGTCAG 
TCGATGGCTA TAGGTACGCA GACTGACTGT GATGCCGGCA CGCAAACTGA TACAGTGGCA 
AGCCATATCG CGCACACATC CGTCAGTTCA CAAGCACATA TGAAAACCAA GTCGTCGCAT 
CGTCGTGCTC GAAGCGAAAA TGACGAGTCC ATGTCTGAGG ATGGCTATGA ATATGTGCGG 
TTTATTCCAC CAAATTGCCC TGAAGGTACG TTCTGGATCA AGCGTCGAAG GACGAAAAAG 
CGCCGAAAAC AGCCACGTAA AAGGATCGTG ATGGTGGAGG AGGTAAAACG TAAAATCCGC 
ACACCCATTA AAGAGGAAGA AGAGCTGCAC GAGCGGAAGA GTCGAGTCCC ACCAAAGAAA 
CCAATGCGGG AGACCAAGAC AAGTATACTG CGGAAACAGC TAAGCGACGA AAGCCGCAAA 
ACTGACGCCA CTTCCAATGG AGAATCGAAG GCGAGCGGGC ATCTGAAATC AGATGTACTT 
ATAGAGGTAA CGGACTCATT CGACGAAAAT AAAAGTACCA GAAACCACAG CACACGAAAG 
ATTCTAGTTG ATAAATATTA CAAACATTCC GATGGGGAAT TCGACGATGG CGAATTCGAC 
GAAGGCGACG ATACAGAGTA TTCTTTTGAC TCCGATGGAG ATGAAATCGT AATCAGAACA 
AGCGCTGTAG TACCTTCACG AGGGCAAAAA AGCGATCAAT TTGTAATGGA ACCAAAAGAG 
AAGCGAAACT TATCATTTTC TCCAGAAAAT ATGGGAGGCA TAAAGCAGCA AAAGTATCCC 
CGTACACAGA CACCAGAGGA GGCAGTCCAG GAGGCACAAT TAAATTCAAT TCCGAGATTA 
TCAAGAAGAG ACAGCAAGAA GCGGGACAAT AATCCCAGAA GACAAACCTC ATCGGAGCCC 
CCGCACAGCC GACTATCCGT TTCTAAGTTC GATTCGATAG CTATTGAAGA CGATAATACA 
CCCCCAGCAG CCACATCATT GATTGATCGA AGCTATCAAA GTGAAGCCGA GTTAGGAAAT 
CTTGAGGGGG CAAGAGAACG CAATGTGCCC AAGTATATGG AGTGGTACTA CAATAAAAAA 
AAATCGTCTC TAAGCGGTCG TACCTCGACG GAGTCATCGA AATCTCAACC CACGATCAAA 
AAAAAGGGTG GTTCATCAGA AAAACGCGTT ACAAAGACGC GCATCGCCGT CCAGAACCAA 
GAAAGTGATT TCCATGATGA TGGTGGAAAA TATAAACCTG AGCCGGCTCC ACGCAGATCC 
GCGCCGAAAG GCAGTCGTTT GCTTAAGGAG GACAGAGCTT TAAATAAGCA GCATAGACCA 
AAAAAAGAGA CTTACACAAA TCATCCACTA CTGCAGCACT CTGAACATAG GTTTGAGCGT 
GAAAGTGCAA TAGAAGTTCC AGCACAGCCT ACCAAACTGC CCCACTACAT GTACCCAGAA 
ACTCCTCCGT ATGCAGCAAC AGCTTCAGAC AAGGAGCTCT CAGCTCAAAG ACAGCAGCCT 
AAGCCGTCCC CGATTAAAGA AAACGAAGTT AAAGTGACGA ACTCGAAAAT AAATCTTCTT 
AAACAAGGTC GTAGCCTCGC ACATCAGACA TCATCGCATG CACAAAAACA GTTAAATGCT 
TCGACGCTCG AGGATGACCA TGATTCAGGC ATAGCTATGA ACTCCCTTTT GAACAGCCTG 
GGTCGTCGCA ATCCGATTGC CGAAAAGAAA AGCGTATTCT CCATTGCCTA CGACGATGTT 
AGTCGGGTCA AAAAGATTCC CTCAGGTGGC GAGTCGCCAC AATACTCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015037615.1
CDS	93..2963
Translation	MLVVPSVRCSNVAEWMGRRDAWSAEKPPNTRTKPTRWEFHDGREHPSENVGRFQDTREKHHVQSAEEQQGRIRKQRTAKDDLHLSFHNSRTNLINDRDVYMEDLVENRDIVDDITRNREIKQLDYAQRQRCETEAHQIDDDSMRRHEIDRGSDMDFPAAQNSMKSKRELFIKEGNVEILQLMTRDKIHDGAALDDDNIYVNVPLKPLGNLSHPQLLMVDNTGKEILMRRFIEEQPDGKQIIREHYQIVPGATYIQSMPNEVQQASNLKVDTYPLVKSGPNSIVYSQMEPEVKVIHTQPEHAVEASSKMRPAISNHSLTQELEQSLKQQNSLLRQILLEKDKLESAYTQEVALETQSLPGQSMAIGTQTDCDAGTQTDTVASHIAHTSVSSQAHMKTKSSHRRARSENDESMSEDGYEYVRFIPPNCPEGTFWIKRRRTKKRRKQPRKRIVMVEEVKRKIRTPIKEEEELHERKSRVPPKKPMRETKTSILRKQLSDESRKTDATSNGESKASGHLKSDVLIEVTDSFDENKSTRNHSTRKILVDKYYKHSDGEFDDGEFDEGDDTEYSFDSDGDEIVIRTSAVVPSRGQKSDQFVMEPKEKRNLSFSPENMGGIKQQKYPRTQTPEEAVQEAQLNSIPRLSRRDSKKRDNNPRRQTSSEPPHSRLSVSKFDSIAIEDDNTPPAATSLIDRSYQSEAELGNLEGARERNVPKYMEWYYNKKKSSLSGRTSTESSKSQPTIKKKGGSSEKRVTKTRIAVQNQESDFHDDGGKYKPEPAPRRSAPKGSRLLKEDRALNKQHRPKKETYTNHPLLQHSEHRFERESAIEVPAQPTKLPHYMYPETPPYAATASDKELSAQRQQPKPSPIKENEVKVTNSKINLLKQGRSLAHQTSSHAQKQLNASTLEDDHDSGIAMNSLLNSLGRRNPIAEKKSVFSIAYDDVSRVKKIPSGGESPQYS

Target ORF information:

RefSeq Version	XM_015182129.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA26445), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015182129.1

ORF Insert Sequence:

ATGCTCGTGG TGCCCTCTGT ACGGTGCAGC AACGTCGCAG AGTGGATGGG AAGACGCGAT 
GCCTGGTCAG CGGAGAAACC CCCGAATACC AGAACGAAAC CTACCCGATG GGAGTTCCAT 
GACGGTCGTG AACATCCTAG CGAAAACGTA GGGAGATTTC AGGACACCCG AGAGAAACAT 
CATGTACAAT CAGCTGAGGA GCAACAGGGA CGCATTAGAA AACAACGTAC AGCCAAAGAC 
GATCTTCATC TTAGTTTTCA TAACTCTAGA ACTAATCTTA TTAATGACCG CGACGTATAC 
ATGGAAGACT TAGTTGAAAA TCGAGATATA GTGGATGACA TTACCCGAAA CCGAGAAATT 
AAACAATTGG ACTATGCGCA AAGACAGCGC TGTGAAACCG AAGCCCACCA AATCGACGAC 
GACTCTATGC GTAGACACGA AATAGATCGC GGTAGCGACA TGGACTTCCC CGCTGCCCAA 
AACAGTATGA AGAGCAAACG CGAACTGTTT ATAAAAGAAG GGAACGTGGA AATTCTTCAA 
CTGATGACCA GAGATAAAAT TCACGATGGT GCTGCACTAG ACGATGACAA CATATATGTA 
AACGTGCCAC TAAAGCCCTT AGGAAACCTG TCACATCCGC AGCTTCTAAT GGTGGACAAT 
ACGGGTAAAG AGATTCTTAT GCGGAGATTT ATTGAAGAGC AACCAGATGG CAAGCAGATA 
ATTCGAGAGC ACTATCAAAT AGTACCAGGA GCAACCTACA TTCAATCCAT GCCGAATGAA 
GTACAACAAG CCTCAAATTT AAAAGTCGAT ACGTATCCAT TAGTCAAATC AGGGCCCAAC 
AGTATTGTTT ATTCTCAAAT GGAACCGGAA GTAAAGGTCA TACATACACA GCCAGAGCAT 
GCTGTTGAAG CTAGTTCAAA GATGCGGCCT GCAATATCGA ACCACTCTCT TACCCAGGAG 
CTGGAGCAGT CTCTTAAGCA ACAGAATTCC CTGCTGCGAC AGATACTGCT CGAAAAGGAC 
AAATTGGAGT CCGCCTATAC ACAGGAAGTG GCATTAGAAA CACAGAGTCT GCCTGGTCAG 
TCGATGGCTA TAGGTACGCA GACTGACTGT GATGCCGGCA CGCAAACTGA TACAGTGGCA 
AGCCATATCG CGCACACATC CGTCAGTTCA CAAGCACATA TGAAAACCAA GTCGTCGCAT 
CGTCGTGCTC GAAGCGAAAA TGACGAGTCC ATGTCTGAGG ATGGCTATGA ATATGTGCGG 
TTTATTCCAC CAAATTGCCC TGAAGGTACG TTCTGGATCA AGCGTCGAAG GACGAAAAAG 
CGCCGAAAAC AGCCACGTAA AAGGATCGTG ATGGTGGAGG AGGTAAAACG TAAAATCCGC 
ACACCCATTA AAGAGGAAGA AGAGCTGCAC GAGCGGAAGA GTCGAGTCCC ACCAAAGAAA 
CCAATGCGGG AGACCAAGAC AAGTATACTG CGGAAACAGC TAAGCGACGA AAGCCGCAAA 
ACTGACGCCA CTTCCAATGG AGAATCGAAG GCGAGCGGGC ATCTGAAATC AGATGTACTT 
ATAGAGGTAA CGGACTCATT CGACGAAAAT AAAAGTACCA GAAACCACAG CACACGAAAG 
ATTCTAGTTG ATAAATATTA CAAACATTCC GATGGGGAAT TCGACGATGG CGAATTCGAC 
GAAGGCGACG ATACAGAGTA TTCTTTTGAC TCCGATGGAG ATGAAATCGT AATCAGAACA 
AGCGCTGTAG TACCTTCACG AGGGCAAAAA AGCGATCAAT TTGTAATGGA ACCAAAAGAG 
AAGCGAAACT TATCATTTTC TCCAGAAAAT ATGGGAGGCA TAAAGCAGCA AAAGTATCCC 
CGTACACAGA CACCAGAGGA GGCAGTCCAG GAGGCACAAT TAAATTCAAT TCCGAGATTA 
TCAAGAAGAG ACAGCAAGAA GCGGGACAAT AATCCCAGAA GACAAACCTC ATCGGAGCCC 
CCGCACAGCC GACTATCCGT TTCTAAGTTC GATTCGATAG CTATTGAAGA CGATAATACA 
CCCCCAGCAG CCACATCATT GATTGATCGA AGCTATCAAA GTGAAGCCGA GTTAGGAAAT 
CTTGAGGGGG CAAGAGAACG CAATGTGCCC AAGTATATGG AGTGGTACTA CAATAAAAAA 
AAATCGTCTC TAAGCGGTCG TACCTCGACG GAGTCATCGA AATCTCAACC CACGATCAAA 
AAAAAGGGTG GTTCATCAGA AAAACGCGTT ACAAAGACGC GCATCGCCGT CCAGAACCAA 
GAAAGTGATT TCCATGATGA TGGTGGAAAA TATAAACCTG AGCCGGCTCC ACGCAGATCC 
GCGCCGAAAG GCAGTCGTTT GCTTAAGGAG GACAGAGCTT TAAATAAGCA GCATAGACCA 
AAAAAAGAGA CTTACACAAA TCATCCACTA CTGCAGCACT CTGAACATAG GTTTGAGCGT 
GAAAGTGCAA TAGAAGTTCC AGCACAGCCT ACCAAACTGC CCCACTACAT GTACCCAGAA 
ACTCCTCCGT ATGCAGCAAC AGCTTCAGAC AAGGAGCTCT CAGCTCAAAG ACAGCAGCCT 
AAGCCGTCCC CGATTAAAGA AAACGAAGTT AAAGTGACGA ACTCGAAAAT AAATCTTCTT 
AAACAAGGTC GTAGCCTCGC ACATCAGACA TCATCGCATG CACAAAAACA GTTAAATGCT 
TCGACGCTCG AGGATGACCA TGATTCAGGC ATAGCTATGA ACTCCCTTTT GAACAGCCTG 
GGTCGTCGCA ATCCGATTGC CGAAAAGAAA AGCGTATTCT CCATTGCCTA CGACGATGTT 
AGTCGGGTCA AAAAGATTCC CTCAGGTGGC GAGTCGCCAC AATACTCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA26445 ( XM_015182127.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015037613.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA26445), mRNA.

Price & Availability↑

CloneID	ODe20427
Clone ID Related Accession (Same CDS sequence)	XM_015182127.1
Accession Version	XM_015182127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAAGG ATCTGTATGA GTTTATTTTG ACGCCGGATC TCTTGGGCTT CACCACCACG 
GCCGTAATTA ATGCGGAAGA CAAAGATGCA ACGGCACCCA ACAACGAAGT GCGGTACGAA 
CTGATCAGTG GCAACTATGA AAACAAATTT AAGCTGGACA AGGTCACCGG GGAATTGACA 
ATTCAGGATA AGATTCACCT GCGGTCCATG ACGGACGCCA GTGAACGAAG ACATCGGGAT 
CCAAGTACAT CGTCCAGTTC TGACGATGAC AACGAAGTGT TTATTTTAAC TGCGCGTGCT 
TACGACCTAG GAGTGCCTGT GCGATTTTCG ACAACAACAA TTCGCATCTA CCCACCTGAA 
AGTCGGAAGC GCATGGTGAC TTTTGTGGTA CCTGGTCACA ACCCAGATAA GGCTAAAACT 
GAAGAAACGC TGTCTGCCAT AACCGGCGGA AAGGTGTTAA TTCACTCAAT ACGACCGCTG 
CGACCTGATG AGCCAGGTGC CAAAGACATT CCTGTCAATA ATCCAGGTAT CAAGGACCGA 
AGTGTCGTCA CCGCTACGGT GCTATATGGC AGCACGTCAT TGGTTGACAT ATCGGATATA 
CAGCAGCGTC TGTCCCAGCA CAATAATTCC TATGCAATTA TGCCGCAAGA CGACGCATTA 
CAGACGAACA CTCAATATAA GGCTGAAAAT AAGGTGCTGT TCTGGCTGCT TATACTTCTG 
GCCACACTGG TCGCATTTAC CGTCCTCATC CTACTTCTTT GCTGCATATG CTCGTGGTGC 
CCTCTGTACG GTGCAGCAAC CAAAAGAATA GTTAATATCA GTAAAGCTGA AGACGATGTG 
CATCTAGTGC ATAGGGAAAT GGCAAATGGA AAACATACAA AATCTGTTCA GGTCGCAGAG 
TGGATGGGAA GACGCGATGC CTGGTCAGCG GAGAAACCCC CGAATACCAG AACGAAACCT 
ACCCGATGGG AGTTCCATGA CGGTCGTGAA CATCCTAGCG AAAACGTAGG GAGATTTCAG 
GACACCCGAG AGAAACATCA TGTACAATCA GCTGAGGAGC AACAGGGACG CATTAGAAAA 
CAACGTACAG CCAAAGACGA TCTTCATCTT AGTTTTCATA ACTCTAGAAC TAATCTTATT 
AATGACCGCG ACGTATACAT GGAAGACTTA GTTGAAAATC GAGATATAGT GGATGACATT 
ACCCGAAACC GAGAAATTAA ACAATTGGAC TATGCGCAAA GACAGCGCTG TGAAACCGAA 
GCCCACCAAA TCGACGACGA CTCTATGCGT AGACACGAAA TAGATCGCGG TAGCGACATG 
GACTTCCCCG CTGCCCAAAA CAGTATGAAG AGCAAACGCG AACTGTTTAT AAAAGAAGGG 
AACGTGGAAA TTCTTCAACT GATGACCAGA GATAAAATTC ACGATGGTGC TGCACTAGAC 
GATGACAACA TATATGTAAA CGTGCCACTA AAGCCCTTAG GAAACCTGTC ACATCCGCAG 
CTTCTAATGG TGGACAATAC GGGTAAAGAG ATTCTTATGC GGAGATTTAT TGAAGAGCAA 
CCAGATGGCA AGCAGATAAT TCGAGAGCAC TATCAAATAG TACCAGGAGC AACCTACATT 
CAATCCATGC CGAATGAAGT ACAACAAGCC TCAAATTTAA AAGTCGATAC GTATCCATTA 
GTCAAATCAG GGCCCAACAG TATTGTTTAT TCTCAAATGG AACCGGAAGT AAAGGTCATA 
CATACACAGC CAGAGCATGC TGTTGAAGCT AGTTCAAAGA TGCGGCCTGC AATATCGAAC 
CACTCTCTTA CCCAGGAGCT GGAGCAGTCT CTTAAGCAAC AGAATTCCCT GCTGCGACAG 
ATACTGCTCG AAAAGGACAA ATTGGAGTCC GCCTATACAC AGGAAGTGGC ATTAGAAACA 
CAGAGTCTGC CTGGTCAGTC GATGGCTATA GGTACGCAGA CTGACTGTGA TGCCGGCACG 
CAAACTGATA CAGTGGCAAG CCATATCGCG CACACATCCG TCAGTTCACA AGCACATATG 
AAAACCAAGT CGTCGCATCG TCGTGCTCGA AGCGAAAATG ACGAGTCCAT GTCTGAGGAT 
GGCTATGAAT ATGTGCGGTT TATTCCACCA AATTGCCCTG AAGGTACGTT CTGGATCAAG 
CGTCGAAGGA CGAAAAAGCG CCGAAAACAG CCACGTAAAA GGATCGTGAT GGTGGAGGAG 
GTAAAACGTA AAATCCGCAC ACCCATTAAA GAGGAAGAAG AGCTGCACGA GCGGAAGAGT 
CGAGTCCCAC CAAAGAAACC AATGCGGGAG ACCAAGACAA GTATACTGCG GAAACAGCTA 
AGCGACGAAA GCCGCAAAAC TGACGCCACT TCCAATGGAG AATCGAAGGC GAGCGGGCAT 
CTGAAATCAG ATGTACTTAT AGAGGTAACG GACTCATTCG ACGAAAATAA AAGTACCAGA 
AACCACAGCA CACGAAAGAT TCTAGTTGAT AAATATTACA AACATTCCGA TGGGGAATTC 
GACGATGGCG AATTCGACGA AGGCGACGAT ACAGAGTATT CTTTTGACTC CGATGGAGAT 
GAAATCGTAA TCAGAACAAG CGCTGTAGTA CCTTCACGAG GGCAAAAAAG CGATCAATTT 
GTAATGGAAC CAAAAGAGAA GCGAAACTTA TCATTTTCTC CAGAAAATAT GGGAGGCATA 
AAGCAGCAAA AGTATCCCCG TACACAGACA CCAGAGGAGG CAGTCCAGGA GGCACAATTA 
AATTCAATTC CGAGATTATC AAGAAGAGAC AGCAAGAAGC GGGACAATAA TCCCAGAAGA 
CAAACCTCAT CGGAGCCCCC GCACAGCCGA CTATCCGTTT CTAAGTTCGA TTCGATAGCT 
ATTGAAGACG ATAATACACC CCCAGCAGCC ACATCATTGA TTGATCGAAG CTATCAAAGT 
GAAGCCGAGT TAGGAAATCT TGAGGGGGCA AGAGAACGCA ATGTGCCCAA GTATATGGAG 
TGGTACTACA ATAAAAAAAA ATCGTCTCTA AGCGGTCGTA CCTCGACGGA GTCATCGAAA 
TCTCAACCCA CGATCAAAAA AAAGGGTGGT TCATCAGAAA AACGCGTTAC AAAGACGCGC 
ATCGCCGTCC AGAACCAAGA AAGTGATTTC CATGATGATG GTGGAAAATA TAAACCTGAG 
CCGGCTCCAC GCAGATCCGC GCCGAAAGGC AGTCGTTTGC TTAAGGAGGA CAGAGCTTTA 
AATAAGCAGC ATAGACCAAA AAAAGAGACT TACACAAATC ATCCACTACT GCAGCACTCT 
GAACATAGGT TTGAGCGTGA AAGTGCAATA GAAGTTCCAG CACAGCCTAC CAAACTGCCC 
CACTACATGT ACCCAGAAAC TCCTCCGTAT GCAGCAACAG CTTCAGACAA GGAGCTCTCA 
GCTCAAAGAC AGCAGCCTAA GCCGTCCCCG ATTAAAGAAA ACGAAGTTAA AGTGACGAAC 
TCGAAAATAA ATCTTCTTAA ACAAGGTCGT AGCCTCGCAC ATCAGACATC ATCGCATGCA 
CAAAAACAGT TAAATGCTTC GACGCTCGAG GATGACCATG ATTCAGGCAT AGCTATGAAC 
TCCCTTTTGA ACAGCCTGGG TCGTCGCAAT CCGATTGCCG AAAAGAAAAG CGTATTCTCC 
ATTGCCTACG ACGATGTTAG TCGGGTCAAA AAGATTCCCT CAGGTGGCGA GTCGCCACAA 
TACTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015037613.1
CDS	6..3734
Translation	MVKDLYEFILTPDLLGFTTTAVINAEDKDATAPNNEVRYELISGNYENKFKLDKVTGELTIQDKIHLRSMTDASERRHRDPSTSSSSDDDNEVFILTARAYDLGVPVRFSTTTIRIYPPESRKRMVTFVVPGHNPDKAKTEETLSAITGGKVLIHSIRPLRPDEPGAKDIPVNNPGIKDRSVVTATVLYGSTSLVDISDIQQRLSQHNNSYAIMPQDDALQTNTQYKAENKVLFWLLILLATLVAFTVLILLLCCICSWCPLYGAATKRIVNISKAEDDVHLVHREMANGKHTKSVQVAEWMGRRDAWSAEKPPNTRTKPTRWEFHDGREHPSENVGRFQDTREKHHVQSAEEQQGRIRKQRTAKDDLHLSFHNSRTNLINDRDVYMEDLVENRDIVDDITRNREIKQLDYAQRQRCETEAHQIDDDSMRRHEIDRGSDMDFPAAQNSMKSKRELFIKEGNVEILQLMTRDKIHDGAALDDDNIYVNVPLKPLGNLSHPQLLMVDNTGKEILMRRFIEEQPDGKQIIREHYQIVPGATYIQSMPNEVQQASNLKVDTYPLVKSGPNSIVYSQMEPEVKVIHTQPEHAVEASSKMRPAISNHSLTQELEQSLKQQNSLLRQILLEKDKLESAYTQEVALETQSLPGQSMAIGTQTDCDAGTQTDTVASHIAHTSVSSQAHMKTKSSHRRARSENDESMSEDGYEYVRFIPPNCPEGTFWIKRRRTKKRRKQPRKRIVMVEEVKRKIRTPIKEEEELHERKSRVPPKKPMRETKTSILRKQLSDESRKTDATSNGESKASGHLKSDVLIEVTDSFDENKSTRNHSTRKILVDKYYKHSDGEFDDGEFDEGDDTEYSFDSDGDEIVIRTSAVVPSRGQKSDQFVMEPKEKRNLSFSPENMGGIKQQKYPRTQTPEEAVQEAQLNSIPRLSRRDSKKRDNNPRRQTSSEPPHSRLSVSKFDSIAIEDDNTPPAATSLIDRSYQSEAELGNLEGARERNVPKYMEWYYNKKKSSLSGRTSTESSKSQPTIKKKGGSSEKRVTKTRIAVQNQESDFHDDGGKYKPEPAPRRSAPKGSRLLKEDRALNKQHRPKKETYTNHPLLQHSEHRFERESAIEVPAQPTKLPHYMYPETPPYAATASDKELSAQRQQPKPSPIKENEVKVTNSKINLLKQGRSLAHQTSSHAQKQLNASTLEDDHDSGIAMNSLLNSLGRRNPIAEKKSVFSIAYDDVSRVKKIPSGGESPQYS

Target ORF information:

RefSeq Version	XM_015182127.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA26445), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015182127.1

ORF Insert Sequence:

ATGGTGAAGG ATCTGTATGA GTTTATTTTG ACGCCGGATC TCTTGGGCTT CACCACCACG 
GCCGTAATTA ATGCGGAAGA CAAAGATGCA ACGGCACCCA ACAACGAAGT GCGGTACGAA 
CTGATCAGTG GCAACTATGA AAACAAATTT AAGCTGGACA AGGTCACCGG GGAATTGACA 
ATTCAGGATA AGATTCACCT GCGGTCCATG ACGGACGCCA GTGAACGAAG ACATCGGGAT 
CCAAGTACAT CGTCCAGTTC TGACGATGAC AACGAAGTGT TTATTTTAAC TGCGCGTGCT 
TACGACCTAG GAGTGCCTGT GCGATTTTCG ACAACAACAA TTCGCATCTA CCCACCTGAA 
AGTCGGAAGC GCATGGTGAC TTTTGTGGTA CCTGGTCACA ACCCAGATAA GGCTAAAACT 
GAAGAAACGC TGTCTGCCAT AACCGGCGGA AAGGTGTTAA TTCACTCAAT ACGACCGCTG 
CGACCTGATG AGCCAGGTGC CAAAGACATT CCTGTCAATA ATCCAGGTAT CAAGGACCGA 
AGTGTCGTCA CCGCTACGGT GCTATATGGC AGCACGTCAT TGGTTGACAT ATCGGATATA 
CAGCAGCGTC TGTCCCAGCA CAATAATTCC TATGCAATTA TGCCGCAAGA CGACGCATTA 
CAGACGAACA CTCAATATAA GGCTGAAAAT AAGGTGCTGT TCTGGCTGCT TATACTTCTG 
GCCACACTGG TCGCATTTAC CGTCCTCATC CTACTTCTTT GCTGCATATG CTCGTGGTGC 
CCTCTGTACG GTGCAGCAAC CAAAAGAATA GTTAATATCA GTAAAGCTGA AGACGATGTG 
CATCTAGTGC ATAGGGAAAT GGCAAATGGA AAACATACAA AATCTGTTCA GGTCGCAGAG 
TGGATGGGAA GACGCGATGC CTGGTCAGCG GAGAAACCCC CGAATACCAG AACGAAACCT 
ACCCGATGGG AGTTCCATGA CGGTCGTGAA CATCCTAGCG AAAACGTAGG GAGATTTCAG 
GACACCCGAG AGAAACATCA TGTACAATCA GCTGAGGAGC AACAGGGACG CATTAGAAAA 
CAACGTACAG CCAAAGACGA TCTTCATCTT AGTTTTCATA ACTCTAGAAC TAATCTTATT 
AATGACCGCG ACGTATACAT GGAAGACTTA GTTGAAAATC GAGATATAGT GGATGACATT 
ACCCGAAACC GAGAAATTAA ACAATTGGAC TATGCGCAAA GACAGCGCTG TGAAACCGAA 
GCCCACCAAA TCGACGACGA CTCTATGCGT AGACACGAAA TAGATCGCGG TAGCGACATG 
GACTTCCCCG CTGCCCAAAA CAGTATGAAG AGCAAACGCG AACTGTTTAT AAAAGAAGGG 
AACGTGGAAA TTCTTCAACT GATGACCAGA GATAAAATTC ACGATGGTGC TGCACTAGAC 
GATGACAACA TATATGTAAA CGTGCCACTA AAGCCCTTAG GAAACCTGTC ACATCCGCAG 
CTTCTAATGG TGGACAATAC GGGTAAAGAG ATTCTTATGC GGAGATTTAT TGAAGAGCAA 
CCAGATGGCA AGCAGATAAT TCGAGAGCAC TATCAAATAG TACCAGGAGC AACCTACATT 
CAATCCATGC CGAATGAAGT ACAACAAGCC TCAAATTTAA AAGTCGATAC GTATCCATTA 
GTCAAATCAG GGCCCAACAG TATTGTTTAT TCTCAAATGG AACCGGAAGT AAAGGTCATA 
CATACACAGC CAGAGCATGC TGTTGAAGCT AGTTCAAAGA TGCGGCCTGC AATATCGAAC 
CACTCTCTTA CCCAGGAGCT GGAGCAGTCT CTTAAGCAAC AGAATTCCCT GCTGCGACAG 
ATACTGCTCG AAAAGGACAA ATTGGAGTCC GCCTATACAC AGGAAGTGGC ATTAGAAACA 
CAGAGTCTGC CTGGTCAGTC GATGGCTATA GGTACGCAGA CTGACTGTGA TGCCGGCACG 
CAAACTGATA CAGTGGCAAG CCATATCGCG CACACATCCG TCAGTTCACA AGCACATATG 
AAAACCAAGT CGTCGCATCG TCGTGCTCGA AGCGAAAATG ACGAGTCCAT GTCTGAGGAT 
GGCTATGAAT ATGTGCGGTT TATTCCACCA AATTGCCCTG AAGGTACGTT CTGGATCAAG 
CGTCGAAGGA CGAAAAAGCG CCGAAAACAG CCACGTAAAA GGATCGTGAT GGTGGAGGAG 
GTAAAACGTA AAATCCGCAC ACCCATTAAA GAGGAAGAAG AGCTGCACGA GCGGAAGAGT 
CGAGTCCCAC CAAAGAAACC AATGCGGGAG ACCAAGACAA GTATACTGCG GAAACAGCTA 
AGCGACGAAA GCCGCAAAAC TGACGCCACT TCCAATGGAG AATCGAAGGC GAGCGGGCAT 
CTGAAATCAG ATGTACTTAT AGAGGTAACG GACTCATTCG ACGAAAATAA AAGTACCAGA 
AACCACAGCA CACGAAAGAT TCTAGTTGAT AAATATTACA AACATTCCGA TGGGGAATTC 
GACGATGGCG AATTCGACGA AGGCGACGAT ACAGAGTATT CTTTTGACTC CGATGGAGAT 
GAAATCGTAA TCAGAACAAG CGCTGTAGTA CCTTCACGAG GGCAAAAAAG CGATCAATTT 
GTAATGGAAC CAAAAGAGAA GCGAAACTTA TCATTTTCTC CAGAAAATAT GGGAGGCATA 
AAGCAGCAAA AGTATCCCCG TACACAGACA CCAGAGGAGG CAGTCCAGGA GGCACAATTA 
AATTCAATTC CGAGATTATC AAGAAGAGAC AGCAAGAAGC GGGACAATAA TCCCAGAAGA 
CAAACCTCAT CGGAGCCCCC GCACAGCCGA CTATCCGTTT CTAAGTTCGA TTCGATAGCT 
ATTGAAGACG ATAATACACC CCCAGCAGCC ACATCATTGA TTGATCGAAG CTATCAAAGT 
GAAGCCGAGT TAGGAAATCT TGAGGGGGCA AGAGAACGCA ATGTGCCCAA GTATATGGAG 
TGGTACTACA ATAAAAAAAA ATCGTCTCTA AGCGGTCGTA CCTCGACGGA GTCATCGAAA 
TCTCAACCCA CGATCAAAAA AAAGGGTGGT TCATCAGAAA AACGCGTTAC AAAGACGCGC 
ATCGCCGTCC AGAACCAAGA AAGTGATTTC CATGATGATG GTGGAAAATA TAAACCTGAG 
CCGGCTCCAC GCAGATCCGC GCCGAAAGGC AGTCGTTTGC TTAAGGAGGA CAGAGCTTTA 
AATAAGCAGC ATAGACCAAA AAAAGAGACT TACACAAATC ATCCACTACT GCAGCACTCT 
GAACATAGGT TTGAGCGTGA AAGTGCAATA GAAGTTCCAG CACAGCCTAC CAAACTGCCC 
CACTACATGT ACCCAGAAAC TCCTCCGTAT GCAGCAACAG CTTCAGACAA GGAGCTCTCA 
GCTCAAAGAC AGCAGCCTAA GCCGTCCCCG ATTAAAGAAA ACGAAGTTAA AGTGACGAAC 
TCGAAAATAA ATCTTCTTAA ACAAGGTCGT AGCCTCGCAC ATCAGACATC ATCGCATGCA 
CAAAAACAGT TAAATGCTTC GACGCTCGAG GATGACCATG ATTCAGGCAT AGCTATGAAC 
TCCCTTTTGA ACAGCCTGGG TCGTCGCAAT CCGATTGCCG AAAAGAAAAG CGTATTCTCC 
ATTGCCTACG ACGATGTTAG TCGGGTCAAA AAGATTCCCT CAGGTGGCGA GTCGCCACAA 
TACTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA26445 ( XM_003736611.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_003736659.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA26445), mRNA.

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CloneID	ODe01657
Clone ID Related Accession (Same CDS sequence)	XM_003736611.2
Accession Version	XM_003736611.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_003736611.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTCCA ATAGTAGCAG TCAATCTGTA AAGAACAAAA CAGTCATACC GCCACGCCAC 
ATGGGAAAGG AACAGAACGG CTTAACTTCA AATGCGGGAC CGATACTCGA AAAGGAATGC 
TACTACTACA CCCCAACTAC CTCCCAGCAA CATCAACAGA TTCAACAAGG CTGGCAATCT 
CTATGGGCTT TTTTTGGGCC CTGGCTGAGT GCAATGACCG CCTATCGATT GCTGACAATC 
AGCCTCCTAA TTGGTATTCT GTGTCCACAT CACATTCAAG GCGCGGATCC TAAATTCGAT 
CCTTCGACGC GCATGCGACT GGTACTGGTG CCGGCGGACG CGCAAGTAAA CTCAGTTATC 
TACAGATTGC GTGCCACTGA CGAGGAGTTC GACTATCCAT TGACCTTTGA GTTTGTGGGT 
GATGCATTTT CGTCCACCGT CAAAGTGGAT TCGCTTCCAT GCACCAAGTA CAATTCGGTT 
TGCCAAGCAA ACATCGTGCT TCAGCGTCGC TTGGAGCCTG GCCGCTATTA CGACTTCCAG 
GTTTCAGTGA AGGACTCAAA AGGTGGCATG GCCACTCAAT TGTGCTCAAT TACAGCAACC 
AATTTTACCA CACCTCATGA CCTCATATTT CCACACAAGC CCGGTATTAT AATGATACCT 
GAGGATGCTA AACGCGGCAC GGAATTAAAT TACGTAATTG CGCGAAAGAA TCCACTGTTT 
CAAAAACCAG TCTACCTCGA GTTATGGGGT TCTCCGCTTT TTGCGATAAG ACAAAAAATT 
GTATCGTCCG AAACAACAGA AGGTACAGTT TTTCTTTTGG GACCATTGGA CTTCGAAAAG 
CAAGCCATGT ACCATCTAAC TATACTTGCC AATGATGCTT ACGCTGAACC TGGCCAAGAT 
AGTCGCAACA TCGCTGGCAT GGAAATTGTA GTCATCGTCC AGGATGTTCA AGACCAACCT 
CCTGTTTTCA CTTCGGCGCC GCCAGTTACC AAACTGCCTA CTGGCATTCT ACCAGGCGAT 
AAGATATTGC AAGTACATGC GGAGGATGGC GACAAAGGAA ACCCGCGGGA GATTCGATTT 
GGATTGGTCT CGGAGAACAA CCCATTTACT TCGTTTTTTG ACATCAATGA CACAAGCGGT 
GAAATATTTT TAATGCGACC TCTTGAAGAT ATTGCTTTTA TAACGCATGT GGGTGATCCA 
GTGCTGCTTA CGGTAATTGC GGAAGAAGTT AAAGTTGGTC GTGACGAACC GCCAGCATTG 
GCATCTACTG TGCAATTGGC GTTCTTTCTG CCAGATCGTA CCAATTCACC GCCATACTTC 
GAAAACGATC ACTACGTTTC GAGGGTAGAC GAAAATGCAC CGCAAGGCAC AGCACTAATA 
TTCGTTGACC CATATGTACC GCGCATCTAT GACGACGACA CTGGAAAAAA TGGAGTTTTC 
TCGCTGACGT TGCTAAACAA TAATGGAACA TTTGAGATAA CACCAAATGT AGCAGAGCGC 
AGTGCATCGT TTCTAGTGCG CGTACGAGAT AACACACTGT TAGACTACGA AATGCGACAC 
TCTGTACAGT TTCAGGTGAT GGCCCAGGAA GTGGGTCCGG CTACTAATCT CACAGCCGTT 
GTAAACGTCA CCGTCTACAT CAATGACGTT AATGATAATG CACCAGTCTT TGACCAGCCC 
TCCTACACAG TGGAGCTTCC CGAGAATATG ACAGTGGGAA CTAAGGTGGT TCAAGTGCAT 
GCCACTGACT TAGACTCGGG ATTGGGTGGC AAAGTACGAT ATACTGCCAT TCTGGGGTAT 
CTAAATACAT CTGTTAACCT CGATGCAGAG AATGGATTAA TCACAATATC GACAAATAAT 
CATGGCTATG ATCGCGAGGT AATGCCGGAA TATCACCTGT ACGTAGAGGC TCGGGACATG 
GATGGTGAAG GTAATCGAGC ACAAGTCCCT CTAATCATTA AGCTAATAGA TGTTAACGAT 
GAGACGCCAA TATTTGACAA GGATCTGTAT GAGTTTATTT TGACGCCGGA TCTCTTGGGC 
TTCACCACCA CGGCCGTAAT TAATGCGGAA GACAAAGATG CAACGGCACC CAACAACGAA 
GTGCGGTACG AACTGATCAG TGGCAACTAT GAAAACAAAT TTAAGCTGGA CAAGGTCACC 
GGGGAATTGA CAATTCAGGA TAAGATTCAC CTGCGGTCCA TGACGGACGC CAGTGAACGA 
AGACATCGGG ATCCAAGTAC ATCGTCCAGT TCTGACGATG ACAACGAAGT GTTTATTTTA 
ACTGCGCGTG CTTACGACCT AGGAGTGCCT GTGCGATTTT CGACAACAAC AATTCGCATC 
TACCCACCTG AAAGTCGGAA GCGCATGGTG ACTTTTGTGG TACCTGGTCA CAACCCAGAT 
AAGGCTAAAA CTGAAGAAAC GCTGTCTGCC ATAACCGGCG GAAAGGTGTT AATTCACTCA 
ATACGACCGC TGCGACCTGA TGAGCCAGGT GCCAAAGACA TTCCTGTCAA TAATCCAGGT 
ATCAAGGACC GAAGTGTCGT CACCGCTACG GTGCTATATG GCAGCACGTC ATTGGTTGAC 
ATATCGGATA TACAGCAGCG TCTGTCCCAG CACAATAATT CCTATGCAAT TATGCCGCAA 
GACGACGCAT TACAGACGAA CAATCTAACA CCACTGCAGA CTCAATATAA GGCTGAAAAT 
AAGGTGCTGT TCTGGCTGCT TATACTTCTG GCCACACTGG TCGCATTTAC CGTCCTCATC 
CTACTTCTTT GCTGCATATG CTCGTGGTGC CCTCTGTACG GTGCAGCAAC CAAAAGAATA 
GTTAATATCA GTAAAGCTGA AGACGATGTG CATCTAGTGC ATAGGGAAAT GGCAAATGGA 
AAACATACAA AATCTGTTCA GGTCGCAGAG TGGATGGGAA GACGCGATGC CTGGTCAGCG 
GAGAAACCCC CGAATACCAG AACGAAACCT ACCCGATGGG AGTTCCATGA CGGTCGTGAA 
CATCCTAGCG AAAACGTAGG GAGATTTCAG GACACCCGAG AGAAACATCA TGTACAATCA 
GCTGAGGAGC AACAGGGACG CATTAGAAAA CAACGTACAG CCAAAGACGA TCTTCATCTT 
AGTTTTCATA ACTCTAGAAC TAATCTTATT AATGACCGCG ACGTATACAT GGAAGACTTA 
GTTGAAAATC GAGATATAGT GGATGACATT ACCCGAAACC GAGAAATTAA ACAATTGGAC 
TATGCGCAAA GACAGCGCTG TGAAACCGAA GCCCACCAAA TCGACGACGA CTCTATGCGT 
AGACACGAAA TAGATCGCGG TAGCGACATG GACTTCCCCG CTGCCCAAAA CAGTATGAAG 
AGCAAACGCG AACTGTTTAT AAAAGAAGGG AACGTGGAAA TTCTTCAACT GATGACCAGA 
GATAAAATTC ACGATGGTGC TGCACTAGAC GATGACAACA TATATGTAAA CGTGCCACTA 
AAGCCCTTAG GAAACCTGTC ACATCCGCAG CTTCTAATGG TGGACAATAC GGGTAAAGAG 
ATTCTTATGC GGAGATTTAT TGAAGAGCAA CCAGATGGCA AGCAGATAAT TCGAGAGCAC 
TATCAAATAG TACCAGGAGC AACCTACATT CAATCCATGC CGAATGAAGT ACAACAAGCC 
TCAAATTTAA AAGTCGATAC GTATCCATTA GTCAAATCAG GGCCCAACAG TATTGTTTAT 
TCTCAAATGG AACCGGAAGT AAAGGTCATA CATACACAGC CAGAGCATGC TGTTGAAGCT 
AGTTCAAAGA TGCGGCCTGC AATATCGAAC CACTCTCTTA CCCAGGAGCT GGAGCAGTCT 
CTTAAGCAAC AGAATTCCCT GCTGCGACAG ATACTGCTCG AAAAGGACAA ATTGGAGTCC 
GCCTATACAC AGGAAGTGGC ATTAGAAACA CAGAGTCTGC CTGGTCAGTC GATGGCTATA 
GGTACGCAGA CTGACTGTGA TGCCGGCACG CAAACTGATA CAGTGGCAAG CCATATCGCG 
CACACATCCG TCAGTTCACA AGCACATATG AAAACCAAGT CGTCGCATCG TCGTGCTCGA 
AGCGAAAATG ACGAGTCCAT GTCTGAGGAT GGCTATGAAT ATGTGCGGTT TATTCCACCA 
AATTGCCCTG AAGGTACGTT CTGGATCAAG CGTCGAAGGA CGAAAAAGCG CCGAAAACAG 
CCACGTAAAA GGATCGTGAT GGTGGAGGAG GTAAAACGTA AAATCCGCAC ACCCATTAAA 
GAGGAAGAAG AGCTGCACGA GCGGAAGAGT CGAGTCCCAC CAAAGAAACC AATGCGGGAG 
ACCAAGACAA GTATACTGCG GAAACAGCTA AGCGACGAAA GCCGCAAAAC TGACGCCACT 
TCCAATGGAG AATCGAAGGC GAGCGGGCAT CTGAAATCAG ATGTACTTAT AGAGGTAACG 
GACTCATTCG ACGAAAATAA AAGTACCAGA AACCACAGCA CACGAAAGAT TCTAGTTGAT 
AAATATTACA AACATTCCGA TGGGGAATTC GACGATGGCG AATTCGACGA AGGCGACGAT 
ACAGAGTATT CTTTTGACTC CGATGGAGAT GAAATCGTAA TCAGAACAAG CGCTGTAGTA 
CCTTCACGAG GGCAAAAAAG CGATCAATTT GTAATGGAAC CAAAAGAGAA GCGAAACTTA 
TCATTTTCTC CAGAAAATAT GGGAGGCATA AAGCAGCAAA AGTATCCCCG TACACAGACA 
CCAGAGGAGG CAGTCCAGGA GGCACAATTA AATTCAATTC CGAGATTATC AAGAAGAGAC 
AGCAAGAAGC GGGACAATAA TCCCAGAAGA CAAACCTCAT CGGAGCCCCC GCACAGCCGA 
CTATCCGTTT CTAAGTTCGA TTCGATAGCT ATTGAAGACG ATAATACACC CCCAGCAGCC 
ACATCATTGA TTGATCGAAG CTATCAAAGT GAAGCCGAGT TAGGAAATCT TGAGGGGGCA 
AGAGAACGCA ATGTGCCCAA GTATATGGAG TGGTACTACA ATAAAAAAAA ATCGTCTCTA 
AGCGGTCGTA CCTCGACGGA GTCATCGAAA TCTCAACCCA CGATCAAAAA AAAGGGTGGT 
TCATCAGAAA AACGCGTTAC AAAGACGCGC ATCGCCGTCC AGAACCAAGA AAGTGATTTC 
CATGATGATG GTGGAAAATA TAAACCTGAG CCGGCTCCAC GCAGATCCGC GCCGAAAGGC 
AGTCGTTTGC TTAAGGAGGA CAGAGCTTTA AATAAGCAGC ATAGACCAAA AAAAGAGACT 
TACACAAATC ATCCACTACT GCAGCACTCT GAACATAGGT TTGAGCGTGA AAGTGCAATA 
GAAGTTCCAG CACAGCCTAC CAAACTGCCC CACTACATGT ACCCAGAAAC TCCTCCGTAT 
GCAGCAACAG CTTCAGACAA GGAGCTCTCA GCTCAAAGAC AGCAGCCTAA GCCGTCCCCG 
ATTAAAGAAA ACGAAGTTAA AGTGACGAAC TCGAAAATAA ATCTTCTTAA ACAAGGTCGT 
AGCCTCGCAC ATCAGACATC ATCGCATGCA CAAAAACAGT TAAATGCTTC GACGCTCGAG 
GATGACCATG ATTCAGGCAT AGCTATGAAC TCCCTTTTGA ACAGCCTGGG TCGTCGCAAT 
CCGATTGCCG AAAAGAAAAG CGTATTCTCC ATTGCCTACG ACGATGTTAG TCGGGTCAAA 
AAGATTCCCT CAGGTGGCGA GTCGCCACAA TACTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003736659.2
CDS	21..5759
Translation	MASNSSSQSVKNKTVIPPRHMGKEQNGLTSNAGPILEKECYYYTPTTSQQHQQIQQGWQSLWAFFGPWLSAMTAYRLLTISLLIGILCPHHIQGADPKFDPSTRMRLVLVPADAQVNSVIYRLRATDEEFDYPLTFEFVGDAFSSTVKVDSLPCTKYNSVCQANIVLQRRLEPGRYYDFQVSVKDSKGGMATQLCSITATNFTTPHDLIFPHKPGIIMIPEDAKRGTELNYVIARKNPLFQKPVYLELWGSPLFAIRQKIVSSETTEGTVFLLGPLDFEKQAMYHLTILANDAYAEPGQDSRNIAGMEIVVIVQDVQDQPPVFTSAPPVTKLPTGILPGDKILQVHAEDGDKGNPREIRFGLVSENNPFTSFFDINDTSGEIFLMRPLEDIAFITHVGDPVLLTVIAEEVKVGRDEPPALASTVQLAFFLPDRTNSPPYFENDHYVSRVDENAPQGTALIFVDPYVPRIYDDDTGKNGVFSLTLLNNNGTFEITPNVAERSASFLVRVRDNTLLDYEMRHSVQFQVMAQEVGPATNLTAVVNVTVYINDVNDNAPVFDQPSYTVELPENMTVGTKVVQVHATDLDSGLGGKVRYTAILGYLNTSVNLDAENGLITISTNNHGYDREVMPEYHLYVEARDMDGEGNRAQVPLIIKLIDVNDETPIFDKDLYEFILTPDLLGFTTTAVINAEDKDATAPNNEVRYELISGNYENKFKLDKVTGELTIQDKIHLRSMTDASERRHRDPSTSSSSDDDNEVFILTARAYDLGVPVRFSTTTIRIYPPESRKRMVTFVVPGHNPDKAKTEETLSAITGGKVLIHSIRPLRPDEPGAKDIPVNNPGIKDRSVVTATVLYGSTSLVDISDIQQRLSQHNNSYAIMPQDDALQTNNLTPLQTQYKAENKVLFWLLILLATLVAFTVLILLLCCICSWCPLYGAATKRIVNISKAEDDVHLVHREMANGKHTKSVQVAEWMGRRDAWSAEKPPNTRTKPTRWEFHDGREHPSENVGRFQDTREKHHVQSAEEQQGRIRKQRTAKDDLHLSFHNSRTNLINDRDVYMEDLVENRDIVDDITRNREIKQLDYAQRQRCETEAHQIDDDSMRRHEIDRGSDMDFPAAQNSMKSKRELFIKEGNVEILQLMTRDKIHDGAALDDDNIYVNVPLKPLGNLSHPQLLMVDNTGKEILMRRFIEEQPDGKQIIREHYQIVPGATYIQSMPNEVQQASNLKVDTYPLVKSGPNSIVYSQMEPEVKVIHTQPEHAVEASSKMRPAISNHSLTQELEQSLKQQNSLLRQILLEKDKLESAYTQEVALETQSLPGQSMAIGTQTDCDAGTQTDTVASHIAHTSVSSQAHMKTKSSHRRARSENDESMSEDGYEYVRFIPPNCPEGTFWIKRRRTKKRRKQPRKRIVMVEEVKRKIRTPIKEEEELHERKSRVPPKKPMRETKTSILRKQLSDESRKTDATSNGESKASGHLKSDVLIEVTDSFDENKSTRNHSTRKILVDKYYKHSDGEFDDGEFDEGDDTEYSFDSDGDEIVIRTSAVVPSRGQKSDQFVMEPKEKRNLSFSPENMGGIKQQKYPRTQTPEEAVQEAQLNSIPRLSRRDSKKRDNNPRRQTSSEPPHSRLSVSKFDSIAIEDDNTPPAATSLIDRSYQSEAELGNLEGARERNVPKYMEWYYNKKKSSLSGRTSTESSKSQPTIKKKGGSSEKRVTKTRIAVQNQESDFHDDGGKYKPEPAPRRSAPKGSRLLKEDRALNKQHRPKKETYTNHPLLQHSEHRFERESAIEVPAQPTKLPHYMYPETPPYAATASDKELSAQRQQPKPSPIKENEVKVTNSKINLLKQGRSLAHQTSSHAQKQLNASTLEDDHDSGIAMNSLLNSLGRRNPIAEKKSVFSIAYDDVSRVKKIPSGGESPQYS

Target ORF information:

RefSeq Version	XM_003736611.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA26445), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003736611.2

ORF Insert Sequence:

ATGGCTTCCA ATAGTAGCAG TCAATCTGTA AAGAACAAAA CAGTCATACC GCCACGCCAC 
ATGGGAAAGG AACAGAACGG CTTAACTTCA AATGCGGGAC CGATACTCGA AAAGGAATGC 
TACTACTACA CCCCAACTAC CTCCCAGCAA CATCAACAGA TTCAACAAGG CTGGCAATCT 
CTATGGGCTT TTTTTGGGCC CTGGCTGAGT GCAATGACCG CCTATCGATT GCTGACAATC 
AGCCTCCTAA TTGGTATTCT GTGTCCACAT CACATTCAAG GCGCGGATCC TAAATTCGAT 
CCTTCGACGC GCATGCGACT GGTACTGGTG CCGGCGGACG CGCAAGTAAA CTCAGTTATC 
TACAGATTGC GTGCCACTGA CGAGGAGTTC GACTATCCAT TGACCTTTGA GTTTGTGGGT 
GATGCATTTT CGTCCACCGT CAAAGTGGAT TCGCTTCCAT GCACCAAGTA CAATTCGGTT 
TGCCAAGCAA ACATCGTGCT TCAGCGTCGC TTGGAGCCTG GCCGCTATTA CGACTTCCAG 
GTTTCAGTGA AGGACTCAAA AGGTGGCATG GCCACTCAAT TGTGCTCAAT TACAGCAACC 
AATTTTACCA CACCTCATGA CCTCATATTT CCACACAAGC CCGGTATTAT AATGATACCT 
GAGGATGCTA AACGCGGCAC GGAATTAAAT TACGTAATTG CGCGAAAGAA TCCACTGTTT 
CAAAAACCAG TCTACCTCGA GTTATGGGGT TCTCCGCTTT TTGCGATAAG ACAAAAAATT 
GTATCGTCCG AAACAACAGA AGGTACAGTT TTTCTTTTGG GACCATTGGA CTTCGAAAAG 
CAAGCCATGT ACCATCTAAC TATACTTGCC AATGATGCTT ACGCTGAACC TGGCCAAGAT 
AGTCGCAACA TCGCTGGCAT GGAAATTGTA GTCATCGTCC AGGATGTTCA AGACCAACCT 
CCTGTTTTCA CTTCGGCGCC GCCAGTTACC AAACTGCCTA CTGGCATTCT ACCAGGCGAT 
AAGATATTGC AAGTACATGC GGAGGATGGC GACAAAGGAA ACCCGCGGGA GATTCGATTT 
GGATTGGTCT CGGAGAACAA CCCATTTACT TCGTTTTTTG ACATCAATGA CACAAGCGGT 
GAAATATTTT TAATGCGACC TCTTGAAGAT ATTGCTTTTA TAACGCATGT GGGTGATCCA 
GTGCTGCTTA CGGTAATTGC GGAAGAAGTT AAAGTTGGTC GTGACGAACC GCCAGCATTG 
GCATCTACTG TGCAATTGGC GTTCTTTCTG CCAGATCGTA CCAATTCACC GCCATACTTC 
GAAAACGATC ACTACGTTTC GAGGGTAGAC GAAAATGCAC CGCAAGGCAC AGCACTAATA 
TTCGTTGACC CATATGTACC GCGCATCTAT GACGACGACA CTGGAAAAAA TGGAGTTTTC 
TCGCTGACGT TGCTAAACAA TAATGGAACA TTTGAGATAA CACCAAATGT AGCAGAGCGC 
AGTGCATCGT TTCTAGTGCG CGTACGAGAT AACACACTGT TAGACTACGA AATGCGACAC 
TCTGTACAGT TTCAGGTGAT GGCCCAGGAA GTGGGTCCGG CTACTAATCT CACAGCCGTT 
GTAAACGTCA CCGTCTACAT CAATGACGTT AATGATAATG CACCAGTCTT TGACCAGCCC 
TCCTACACAG TGGAGCTTCC CGAGAATATG ACAGTGGGAA CTAAGGTGGT TCAAGTGCAT 
GCCACTGACT TAGACTCGGG ATTGGGTGGC AAAGTACGAT ATACTGCCAT TCTGGGGTAT 
CTAAATACAT CTGTTAACCT CGATGCAGAG AATGGATTAA TCACAATATC GACAAATAAT 
CATGGCTATG ATCGCGAGGT AATGCCGGAA TATCACCTGT ACGTAGAGGC TCGGGACATG 
GATGGTGAAG GTAATCGAGC ACAAGTCCCT CTAATCATTA AGCTAATAGA TGTTAACGAT 
GAGACGCCAA TATTTGACAA GGATCTGTAT GAGTTTATTT TGACGCCGGA TCTCTTGGGC 
TTCACCACCA CGGCCGTAAT TAATGCGGAA GACAAAGATG CAACGGCACC CAACAACGAA 
GTGCGGTACG AACTGATCAG TGGCAACTAT GAAAACAAAT TTAAGCTGGA CAAGGTCACC 
GGGGAATTGA CAATTCAGGA TAAGATTCAC CTGCGGTCCA TGACGGACGC CAGTGAACGA 
AGACATCGGG ATCCAAGTAC ATCGTCCAGT TCTGACGATG ACAACGAAGT GTTTATTTTA 
ACTGCGCGTG CTTACGACCT AGGAGTGCCT GTGCGATTTT CGACAACAAC AATTCGCATC 
TACCCACCTG AAAGTCGGAA GCGCATGGTG ACTTTTGTGG TACCTGGTCA CAACCCAGAT 
AAGGCTAAAA CTGAAGAAAC GCTGTCTGCC ATAACCGGCG GAAAGGTGTT AATTCACTCA 
ATACGACCGC TGCGACCTGA TGAGCCAGGT GCCAAAGACA TTCCTGTCAA TAATCCAGGT 
ATCAAGGACC GAAGTGTCGT CACCGCTACG GTGCTATATG GCAGCACGTC ATTGGTTGAC 
ATATCGGATA TACAGCAGCG TCTGTCCCAG CACAATAATT CCTATGCAAT TATGCCGCAA 
GACGACGCAT TACAGACGAA CAATCTAACA CCACTGCAGA CTCAATATAA GGCTGAAAAT 
AAGGTGCTGT TCTGGCTGCT TATACTTCTG GCCACACTGG TCGCATTTAC CGTCCTCATC 
CTACTTCTTT GCTGCATATG CTCGTGGTGC CCTCTGTACG GTGCAGCAAC CAAAAGAATA 
GTTAATATCA GTAAAGCTGA AGACGATGTG CATCTAGTGC ATAGGGAAAT GGCAAATGGA 
AAACATACAA AATCTGTTCA GGTCGCAGAG TGGATGGGAA GACGCGATGC CTGGTCAGCG 
GAGAAACCCC CGAATACCAG AACGAAACCT ACCCGATGGG AGTTCCATGA CGGTCGTGAA 
CATCCTAGCG AAAACGTAGG GAGATTTCAG GACACCCGAG AGAAACATCA TGTACAATCA 
GCTGAGGAGC AACAGGGACG CATTAGAAAA CAACGTACAG CCAAAGACGA TCTTCATCTT 
AGTTTTCATA ACTCTAGAAC TAATCTTATT AATGACCGCG ACGTATACAT GGAAGACTTA 
GTTGAAAATC GAGATATAGT GGATGACATT ACCCGAAACC GAGAAATTAA ACAATTGGAC 
TATGCGCAAA GACAGCGCTG TGAAACCGAA GCCCACCAAA TCGACGACGA CTCTATGCGT 
AGACACGAAA TAGATCGCGG TAGCGACATG GACTTCCCCG CTGCCCAAAA CAGTATGAAG 
AGCAAACGCG AACTGTTTAT AAAAGAAGGG AACGTGGAAA TTCTTCAACT GATGACCAGA 
GATAAAATTC ACGATGGTGC TGCACTAGAC GATGACAACA TATATGTAAA CGTGCCACTA 
AAGCCCTTAG GAAACCTGTC ACATCCGCAG CTTCTAATGG TGGACAATAC GGGTAAAGAG 
ATTCTTATGC GGAGATTTAT TGAAGAGCAA CCAGATGGCA AGCAGATAAT TCGAGAGCAC 
TATCAAATAG TACCAGGAGC AACCTACATT CAATCCATGC CGAATGAAGT ACAACAAGCC 
TCAAATTTAA AAGTCGATAC GTATCCATTA GTCAAATCAG GGCCCAACAG TATTGTTTAT 
TCTCAAATGG AACCGGAAGT AAAGGTCATA CATACACAGC CAGAGCATGC TGTTGAAGCT 
AGTTCAAAGA TGCGGCCTGC AATATCGAAC CACTCTCTTA CCCAGGAGCT GGAGCAGTCT 
CTTAAGCAAC AGAATTCCCT GCTGCGACAG ATACTGCTCG AAAAGGACAA ATTGGAGTCC 
GCCTATACAC AGGAAGTGGC ATTAGAAACA CAGAGTCTGC CTGGTCAGTC GATGGCTATA 
GGTACGCAGA CTGACTGTGA TGCCGGCACG CAAACTGATA CAGTGGCAAG CCATATCGCG 
CACACATCCG TCAGTTCACA AGCACATATG AAAACCAAGT CGTCGCATCG TCGTGCTCGA 
AGCGAAAATG ACGAGTCCAT GTCTGAGGAT GGCTATGAAT ATGTGCGGTT TATTCCACCA 
AATTGCCCTG AAGGTACGTT CTGGATCAAG CGTCGAAGGA CGAAAAAGCG CCGAAAACAG 
CCACGTAAAA GGATCGTGAT GGTGGAGGAG GTAAAACGTA AAATCCGCAC ACCCATTAAA 
GAGGAAGAAG AGCTGCACGA GCGGAAGAGT CGAGTCCCAC CAAAGAAACC AATGCGGGAG 
ACCAAGACAA GTATACTGCG GAAACAGCTA AGCGACGAAA GCCGCAAAAC TGACGCCACT 
TCCAATGGAG AATCGAAGGC GAGCGGGCAT CTGAAATCAG ATGTACTTAT AGAGGTAACG 
GACTCATTCG ACGAAAATAA AAGTACCAGA AACCACAGCA CACGAAAGAT TCTAGTTGAT 
AAATATTACA AACATTCCGA TGGGGAATTC GACGATGGCG AATTCGACGA AGGCGACGAT 
ACAGAGTATT CTTTTGACTC CGATGGAGAT GAAATCGTAA TCAGAACAAG CGCTGTAGTA 
CCTTCACGAG GGCAAAAAAG CGATCAATTT GTAATGGAAC CAAAAGAGAA GCGAAACTTA 
TCATTTTCTC CAGAAAATAT GGGAGGCATA AAGCAGCAAA AGTATCCCCG TACACAGACA 
CCAGAGGAGG CAGTCCAGGA GGCACAATTA AATTCAATTC CGAGATTATC AAGAAGAGAC 
AGCAAGAAGC GGGACAATAA TCCCAGAAGA CAAACCTCAT CGGAGCCCCC GCACAGCCGA 
CTATCCGTTT CTAAGTTCGA TTCGATAGCT ATTGAAGACG ATAATACACC CCCAGCAGCC 
ACATCATTGA TTGATCGAAG CTATCAAAGT GAAGCCGAGT TAGGAAATCT TGAGGGGGCA 
AGAGAACGCA ATGTGCCCAA GTATATGGAG TGGTACTACA ATAAAAAAAA ATCGTCTCTA 
AGCGGTCGTA CCTCGACGGA GTCATCGAAA TCTCAACCCA CGATCAAAAA AAAGGGTGGT 
TCATCAGAAA AACGCGTTAC AAAGACGCGC ATCGCCGTCC AGAACCAAGA AAGTGATTTC 
CATGATGATG GTGGAAAATA TAAACCTGAG CCGGCTCCAC GCAGATCCGC GCCGAAAGGC 
AGTCGTTTGC TTAAGGAGGA CAGAGCTTTA AATAAGCAGC ATAGACCAAA AAAAGAGACT 
TACACAAATC ATCCACTACT GCAGCACTCT GAACATAGGT TTGAGCGTGA AAGTGCAATA 
GAAGTTCCAG CACAGCCTAC CAAACTGCCC CACTACATGT ACCCAGAAAC TCCTCCGTAT 
GCAGCAACAG CTTCAGACAA GGAGCTCTCA GCTCAAAGAC AGCAGCCTAA GCCGTCCCCG 
ATTAAAGAAA ACGAAGTTAA AGTGACGAAC TCGAAAATAA ATCTTCTTAA ACAAGGTCGT 
AGCCTCGCAC ATCAGACATC ATCGCATGCA CAAAAACAGT TAAATGCTTC GACGCTCGAG 
GATGACCATG ATTCAGGCAT AGCTATGAAC TCCCTTTTGA ACAGCCTGGG TCGTCGCAAT 
CCGATTGCCG AAAAGAAAAG CGTATTCTCC ATTGCCTACG ACGATGTTAG TCGGGTCAAA 
AAGATTCCCT CAGGTGGCGA GTCGCCACAA TACTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA26445 ( XM_002137568.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002137604.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA26445), mRNA.

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CloneID	ODe19586
Clone ID Related Accession (Same CDS sequence)	XM_002137568.2
Accession Version	XM_002137568.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_002137568.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCGAT GGATAACACA TATTGCAGCT GTTCTTGAAC ATTCAACAGA AATGGCTTCC 
AATAGTAGCA GTCAATCTGT AAAGAACAAA ACAGTCATAC CGCCACGCCA CATGGGAAAG 
GAACAGAACG GCTTAACTTC AAATGCGGGA CCGATACTCG AAAAGGAATG CTACTACTAC 
ACCCCAACTA CCTCCCAGCA ACATCAACAG ATTCAACAAG GCTGGCAATC TCTATGGGCT 
TTTTTTGGGC CCTGGCTGAG TGCAATGACC GCCTATCGAT TGCTGACAAT CAGCCTCCTA 
ATTGGTATTC TGTGTCCACA TCACATTCAA GGCGCGGATC CTAAATTCGA TCCTTCGACG 
CGCATGCGAC TGGTACTGGT GCCGGCGGAC GCGCAAGTAA ACTCAGTTAT CTACAGATTG 
CGTGCCACTG ACGAGGAGTT CGACTATCCA TTGACCTTTG AGTTTGTGGG TGATGCATTT 
TCGTCCACCG TCAAAGTGGA TTCGCTTCCA TGCACCAAGT ACAATTCGGT TTGCCAAGCA 
AACATCGTGC TTCAGCGTCG CTTGGAGCCT GGCCGCTATT ACGACTTCCA GGTTTCAGTG 
AAGGACTCAA AAGGTGGCAT GGCCACTCAA TTGTGCTCAA TTACAGCAAC CAATTTTACC 
ACACCTCATG ACCTCATATT TCCACACAAG CCCGGTATTA TAATGATACC TGAGGATGCT 
AAACGCGGCA CGGAATTAAA TTACGTAATT GCGCGAAAGA ATCCACTGTT TCAAAAACCA 
GTCTACCTCG AGTTATGGGG TTCTCCGCTT TTTGCGATAA GACAAAAAAT TGTATCGTCC 
GAAACAACAG AAGGTACAGT TTTTCTTTTG GGACCATTGG ACTTCGAAAA GCAAGCCATG 
TACCATCTAA CTATACTTGC CAATGATGCT TACGCTGAAC CTGGCCAAGA TAGTCGCAAC 
ATCGCTGGCA TGGAAATTGT AGTCATCGTC CAGGATGTTC AAGACCAACC TCCTGTTTTC 
ACTTCGGCGC CGCCAGTTAC CAAACTGCCT ACTGGCATTC TACCAGGCGA TAAGATATTG 
CAAGTACATG CGGAGGATGG CGACAAAGGA AACCCGCGGG AGATTCGATT TGGATTGGTC 
TCGGAGAACA ACCCATTTAC TTCGTTTTTT GACATCAATG ACACAAGCGG TGAAATATTT 
TTAATGCGAC CTCTTGAAGA TATTGCTTTT ATAACGCATG TGGGTGATCC AGTGCTGCTT 
ACGGTAATTG CGGAAGAAGT TAAAGTTGGT CGTGACGAAC CGCCAGCATT GGCATCTACT 
GTGCAATTGG CGTTCTTTCT GCCAGATCGT ACCAATTCAC CGCCATACTT CGAAAACGAT 
CACTACGTTT CGAGGGTAGA CGAAAATGCA CCGCAAGGCA CAGCACTAAT ATTCGTTGAC 
CCATATGTAC CGCGCATCTA TGACGACGAC ACTGGAAAAA ATGGAGTTTT CTCGCTGACG 
TTGCTAAACA ATAATGGAAC ATTTGAGATA ACACCAAATG TAGCAGAGCG CAGTGCATCG 
TTTCTAGTGC GCGTACGAGA TAACACACTG TTAGACTACG AAATGCGACA CTCTGTACAG 
TTTCAGGTGA TGGCCCAGGA AGTGGGTCCG GCTACTAATC TCACAGCCGT TGTAAACGTC 
ACCGTCTACA TCAATGACGT TAATGATAAT GCACCAGTCT TTGACCAGCC CTCCTACACA 
GTGGAGCTTC CCGAGAATAT GACAGTGGGA ACTAAGGTGG TTCAAGTGCA TGCCACTGAC 
TTAGACTCGG GATTGGGTGG CAAAGTACGA TATACTGCCA TTCTGGGGTA TCTAAATACA 
TCTGTTAACC TCGATGCAGA GAATGGATTA ATCACAATAT CGACAAATAA TCATGGCTAT 
GATCGCGAGG TAATGCCGGA ATATCACCTG TACGTAGAGG CTCGGGACAT GGATGGTGAA 
GGTAATCGAG CACAAGTCCC TCTAATCATT AAGCTAATAG ATGTTAACGA TGAGACGCCA 
ATATTTGACA AGGATCTGTA TGAGTTTATT TTGACGCCGG ATCTCTTGGG CTTCACCACC 
ACGGCCGTAA TTAATGCGGA AGACAAAGAT GCAACGGCAC CCAACAACGA AGTGCGGTAC 
GAACTGATCA GTGGCAACTA TGAAAACAAA TTTAAGCTGG ACAAGGTCAC CGGGGAATTG 
ACAATTCAGG ATAAGATTCA CCTGCGGTCC ATGACGGACG CCAGTGAACG AAGACATCGG 
GATCCAAGTA CATCGTCCAG TTCTGACGAT GACAACGAAG TGTTTATTTT AACTGCGCGT 
GCTTACGACC TAGGAGTGCC TGTGCGATTT TCGACAACAA CAATTCGCAT CTACCCACCT 
GAAAGTCGGA AGCGCATGGT GACTTTTGTG GTACCTGGTC ACAACCCAGA TAAGGCTAAA 
ACTGAAGAAA CGCTGTCTGC CATAACCGGC GGAAAGGTGT TAATTCACTC AATACGACCG 
CTGCGACCTG ATGAGCCAGG TGCCAAAGAC ATTCCTGTCA ATAATCCAGG TATCAAGGAC 
CGAAGTGTCG TCACCGCTAC GGTGCTATAT GGCAGCACGT CATTGGTTGA CATATCGGAT 
ATACAGCAGC GTCTGTCCCA GCACAATAAT TCCTATGCAA TTATGCCGCA AGACGACGCA 
TTACAGACGA ACAATCTAAC ACCACTGCAG ACTCAATATA AGGCTGAAAA TAAGGTGCTG 
TTCTGGCTGC TTATACTTCT GGCCACACTG GTCGCATTTA CCGTCCTCAT CCTACTTCTT 
TGCTGCATAT GCTCGTGGTG CCCTCTGTAC GGTGCAGCAA CCAAAAGAAT AGTTAATATC 
AGTAAAGCTG AAGACGATGT GCATCTAGTG CATAGGGAAA TGGCAAATGG AAAACATACA 
AAATCTGTTC AGGTCGCAGA GTGGATGGGA AGACGCGATG CCTGGTCAGC GGAGAAACCC 
CCGAATACCA GAACGAAACC TACCCGATGG GAGTTCCATG ACGGTCGTGA ACATCCTAGC 
GAAAACGTAG GGAGATTTCA GGACACCCGA GAGAAACATC ATGTACAATC AGCTGAGGAG 
CAACAGGGAC GCATTAGAAA ACAACGTACA GCCAAAGACG ATCTTCATCT TAGTTTTCAT 
AACTCTAGAA CTAATCTTAT TAATGACCGC GACGTATACA TGGAAGACTT AGTTGAAAAT 
CGAGATATAG TGGATGACAT TACCCGAAAC CGAGAAATTA AACAATTGGA CTATGCGCAA 
AGACAGCGCT GTGAAACCGA AGCCCACCAA ATCGACGACG ACTCTATGCG TAGACACGAA 
ATAGATCGCG GTAGCGACAT GGACTTCCCC GCTGCCCAAA ACAGTATGAA GAGCAAACGC 
GAACTGTTTA TAAAAGAAGG GAACGTGGAA ATTCTTCAAC TGATGACCAG AGATAAAATT 
CACGATGGTG CTGCACTAGA CGATGACAAC ATATATGTAA ACGTGCCACT AAAGCCCTTA 
GGAAACCTGT CACATCCGCA GCTTCTAATG GTGGACAATA CGGGTAAAGA GATTCTTATG 
CGGAGATTTA TTGAAGAGCA ACCAGATGGC AAGCAGATAA TTCGAGAGCA CTATCAAATA 
GTACCAGGAG CAACCTACAT TCAATCCATG CCGAATGAAG TACAACAAGC CTCAAATTTA 
AAAGTCGATA CGTATCCATT AGTCAAATCA GGGCCCAACA GTATTGTTTA TTCTCAAATG 
GAACCGGAAG TAAAGGTCAT ACATACACAG CCAGAGCATG CTGTTGAAGC TAGTTCAAAG 
ATGCGGCCTG CAATATCGAA CCACTCTCTT ACCCAGGAGC TGGAGCAGTC TCTTAAGCAA 
CAGAATTCCC TGCTGCGACA GATACTGCTC GAAAAGGACA AATTGGAGTC CGCCTATACA 
CAGGAAGTGG CATTAGAAAC ACAGAGTCTG CCTGGTCAGT CGATGGCTAT AGGTACGCAG 
ACTGACTGTG ATGCCGGCAC GCAAACTGAT ACAGTGGCAA GCCATATCGC GCACACATCC 
GTCAGTTCAC AAGCACATAT GAAAACCAAG TCGTCGCATC GTCGTGCTCG AAGCGAAAAT 
GACGAGTCCA TGTCTGAGGA TGGCTATGAA TATGTGCGGT TTATTCCACC AAATTGCCCT 
GAAGGTACGT TCTGGATCAA GCGTCGAAGG ACGAAAAAGC GCCGAAAACA GCCACGTAAA 
AGGATCGTGA TGGTGGAGGA GGTAAAACGT AAAATCCGCA CACCCATTAA AGAGGAAGAA 
GAGCTGCACG AGCGGAAGAG TCGAGTCCCA CCAAAGAAAC CAATGCGGGA GACCAAGACA 
AGTATACTGC GGAAACAGCT AAGCGACGAA AGCCGCAAAA CTGACGCCAC TTCCAATGGA 
GAATCGAAGG CGAGCGGGCA TCTGAAATCA GATGTACTTA TAGAGGTAAC GGACTCATTC 
GACGAAAATA AAAGTACCAG AAACCACAGC ACACGAAAGA TTCTAGTTGA TAAATATTAC 
AAACATTCCG ATGGGGAATT CGACGATGGC GAATTCGACG AAGGCGACGA TACAGAGTAT 
TCTTTTGACT CCGATGGAGA TGAAATCGTA ATCAGAACAA GCGCTGTAGT ACCTTCACGA 
GGGCAAAAAA GCGATCAATT TGTAATGGAA CCAAAAGAGA AGCGAAACTT ATCATTTTCT 
CCAGAAAATA TGGGAGGCAT AAAGCAGCAA AAGTATCCCC GTACACAGAC ACCAGAGGAG 
GCAGTCCAGG AGGCACAATT AAATTCAATT CCGAGATTAT CAAGAAGAGA CAGCAAGAAG 
CGGGACAATA ATCCCAGAAG ACAAACCTCA TCGGAGCCCC CGCACAGCCG ACTATCCGTT 
TCTAAGTTCG ATTCGATAGC TATTGAAGAC GATAATACAC CCCCAGCAGC CACATCATTG 
ATTGATCGAA GCTATCAAAG TGAAGCCGAG TTAGGAAATC TTGAGGGGGC AAGAGAACGC 
AATGTGCCCA AGTATATGGA GTGGTACTAC AATAAAAAAA AATCGTCTCT AAGCGGTCGT 
ACCTCGACGG AGTCATCGAA ATCTCAACCC ACGATCAAAA AAAAGGGTGG TTCATCAGAA 
AAACGCGTTA CAAAGACGCG CATCGCCGTC CAGAACCAAG AAAGTGATTT CCATGATGAT 
GGTGGAAAAT ATAAACCTGA GCCGGCTCCA CGCAGATCCG CGCCGAAAGG CAGTCGTTTG 
CTTAAGGAGG ACAGAGCTTT AAATAAGCAG CATAGACCAA AAAAAGAGAC TTACACAAAT 
CATCCACTAC TGCAGCACTC TGAACATAGG TTTGAGCGTG AAAGTGCAAT AGAAGTTCCA 
GCACAGCCTA CCAAACTGCC CCACTACATG TACCCAGAAA CTCCTCCGTA TGCAGCAACA 
GCTTCAGACA AGGAGCTCTC AGCTCAAAGA CAGCAGCCTA AGCCGTCCCC GATTAAAGAA 
AACGAAGTTA AAGTGACGAA CTCGAAAATA AATCTTCTTA AACAAGGTCG TAGCCTCGCA 
CATCAGACAT CATCGCATGC ACAAAAACAG TTAAATGCTT CGACGCTCGA GGATGACCAT 
GATTCAGGCA TAGCTATGAA CTCCCTTTTG AACAGCCTGG GTCGTCGCAA TCCGATTGCC 
GAAAAGAAAA GCGTATTCTC CATTGCCTAC GACGATGTTA GTCGGGTCAA AAAGATTCCC 
TCAGGTGGCG AGTCGCCACA ATACTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002137604.2
CDS	421..6210
Translation	MFRWITHIAAVLEHSTEMASNSSSQSVKNKTVIPPRHMGKEQNGLTSNAGPILEKECYYYTPTTSQQHQQIQQGWQSLWAFFGPWLSAMTAYRLLTISLLIGILCPHHIQGADPKFDPSTRMRLVLVPADAQVNSVIYRLRATDEEFDYPLTFEFVGDAFSSTVKVDSLPCTKYNSVCQANIVLQRRLEPGRYYDFQVSVKDSKGGMATQLCSITATNFTTPHDLIFPHKPGIIMIPEDAKRGTELNYVIARKNPLFQKPVYLELWGSPLFAIRQKIVSSETTEGTVFLLGPLDFEKQAMYHLTILANDAYAEPGQDSRNIAGMEIVVIVQDVQDQPPVFTSAPPVTKLPTGILPGDKILQVHAEDGDKGNPREIRFGLVSENNPFTSFFDINDTSGEIFLMRPLEDIAFITHVGDPVLLTVIAEEVKVGRDEPPALASTVQLAFFLPDRTNSPPYFENDHYVSRVDENAPQGTALIFVDPYVPRIYDDDTGKNGVFSLTLLNNNGTFEITPNVAERSASFLVRVRDNTLLDYEMRHSVQFQVMAQEVGPATNLTAVVNVTVYINDVNDNAPVFDQPSYTVELPENMTVGTKVVQVHATDLDSGLGGKVRYTAILGYLNTSVNLDAENGLITISTNNHGYDREVMPEYHLYVEARDMDGEGNRAQVPLIIKLIDVNDETPIFDKDLYEFILTPDLLGFTTTAVINAEDKDATAPNNEVRYELISGNYENKFKLDKVTGELTIQDKIHLRSMTDASERRHRDPSTSSSSDDDNEVFILTARAYDLGVPVRFSTTTIRIYPPESRKRMVTFVVPGHNPDKAKTEETLSAITGGKVLIHSIRPLRPDEPGAKDIPVNNPGIKDRSVVTATVLYGSTSLVDISDIQQRLSQHNNSYAIMPQDDALQTNNLTPLQTQYKAENKVLFWLLILLATLVAFTVLILLLCCICSWCPLYGAATKRIVNISKAEDDVHLVHREMANGKHTKSVQVAEWMGRRDAWSAEKPPNTRTKPTRWEFHDGREHPSENVGRFQDTREKHHVQSAEEQQGRIRKQRTAKDDLHLSFHNSRTNLINDRDVYMEDLVENRDIVDDITRNREIKQLDYAQRQRCETEAHQIDDDSMRRHEIDRGSDMDFPAAQNSMKSKRELFIKEGNVEILQLMTRDKIHDGAALDDDNIYVNVPLKPLGNLSHPQLLMVDNTGKEILMRRFIEEQPDGKQIIREHYQIVPGATYIQSMPNEVQQASNLKVDTYPLVKSGPNSIVYSQMEPEVKVIHTQPEHAVEASSKMRPAISNHSLTQELEQSLKQQNSLLRQILLEKDKLESAYTQEVALETQSLPGQSMAIGTQTDCDAGTQTDTVASHIAHTSVSSQAHMKTKSSHRRARSENDESMSEDGYEYVRFIPPNCPEGTFWIKRRRTKKRRKQPRKRIVMVEEVKRKIRTPIKEEEELHERKSRVPPKKPMRETKTSILRKQLSDESRKTDATSNGESKASGHLKSDVLIEVTDSFDENKSTRNHSTRKILVDKYYKHSDGEFDDGEFDEGDDTEYSFDSDGDEIVIRTSAVVPSRGQKSDQFVMEPKEKRNLSFSPENMGGIKQQKYPRTQTPEEAVQEAQLNSIPRLSRRDSKKRDNNPRRQTSSEPPHSRLSVSKFDSIAIEDDNTPPAATSLIDRSYQSEAELGNLEGARERNVPKYMEWYYNKKKSSLSGRTSTESSKSQPTIKKKGGSSEKRVTKTRIAVQNQESDFHDDGGKYKPEPAPRRSAPKGSRLLKEDRALNKQHRPKKETYTNHPLLQHSEHRFERESAIEVPAQPTKLPHYMYPETPPYAATASDKELSAQRQQPKPSPIKENEVKVTNSKINLLKQGRSLAHQTSSHAQKQLNASTLEDDHDSGIAMNSLLNSLGRRNPIAEKKSVFSIAYDDVSRVKKIPSGGESPQYS

Target ORF information:

RefSeq Version	XM_002137568.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA26445), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002137568.2

ORF Insert Sequence:

ATGTTTCGAT GGATAACACA TATTGCAGCT GTTCTTGAAC ATTCAACAGA AATGGCTTCC 
AATAGTAGCA GTCAATCTGT AAAGAACAAA ACAGTCATAC CGCCACGCCA CATGGGAAAG 
GAACAGAACG GCTTAACTTC AAATGCGGGA CCGATACTCG AAAAGGAATG CTACTACTAC 
ACCCCAACTA CCTCCCAGCA ACATCAACAG ATTCAACAAG GCTGGCAATC TCTATGGGCT 
TTTTTTGGGC CCTGGCTGAG TGCAATGACC GCCTATCGAT TGCTGACAAT CAGCCTCCTA 
ATTGGTATTC TGTGTCCACA TCACATTCAA GGCGCGGATC CTAAATTCGA TCCTTCGACG 
CGCATGCGAC TGGTACTGGT GCCGGCGGAC GCGCAAGTAA ACTCAGTTAT CTACAGATTG 
CGTGCCACTG ACGAGGAGTT CGACTATCCA TTGACCTTTG AGTTTGTGGG TGATGCATTT 
TCGTCCACCG TCAAAGTGGA TTCGCTTCCA TGCACCAAGT ACAATTCGGT TTGCCAAGCA 
AACATCGTGC TTCAGCGTCG CTTGGAGCCT GGCCGCTATT ACGACTTCCA GGTTTCAGTG 
AAGGACTCAA AAGGTGGCAT GGCCACTCAA TTGTGCTCAA TTACAGCAAC CAATTTTACC 
ACACCTCATG ACCTCATATT TCCACACAAG CCCGGTATTA TAATGATACC TGAGGATGCT 
AAACGCGGCA CGGAATTAAA TTACGTAATT GCGCGAAAGA ATCCACTGTT TCAAAAACCA 
GTCTACCTCG AGTTATGGGG TTCTCCGCTT TTTGCGATAA GACAAAAAAT TGTATCGTCC 
GAAACAACAG AAGGTACAGT TTTTCTTTTG GGACCATTGG ACTTCGAAAA GCAAGCCATG 
TACCATCTAA CTATACTTGC CAATGATGCT TACGCTGAAC CTGGCCAAGA TAGTCGCAAC 
ATCGCTGGCA TGGAAATTGT AGTCATCGTC CAGGATGTTC AAGACCAACC TCCTGTTTTC 
ACTTCGGCGC CGCCAGTTAC CAAACTGCCT ACTGGCATTC TACCAGGCGA TAAGATATTG 
CAAGTACATG CGGAGGATGG CGACAAAGGA AACCCGCGGG AGATTCGATT TGGATTGGTC 
TCGGAGAACA ACCCATTTAC TTCGTTTTTT GACATCAATG ACACAAGCGG TGAAATATTT 
TTAATGCGAC CTCTTGAAGA TATTGCTTTT ATAACGCATG TGGGTGATCC AGTGCTGCTT 
ACGGTAATTG CGGAAGAAGT TAAAGTTGGT CGTGACGAAC CGCCAGCATT GGCATCTACT 
GTGCAATTGG CGTTCTTTCT GCCAGATCGT ACCAATTCAC CGCCATACTT CGAAAACGAT 
CACTACGTTT CGAGGGTAGA CGAAAATGCA CCGCAAGGCA CAGCACTAAT ATTCGTTGAC 
CCATATGTAC CGCGCATCTA TGACGACGAC ACTGGAAAAA ATGGAGTTTT CTCGCTGACG 
TTGCTAAACA ATAATGGAAC ATTTGAGATA ACACCAAATG TAGCAGAGCG CAGTGCATCG 
TTTCTAGTGC GCGTACGAGA TAACACACTG TTAGACTACG AAATGCGACA CTCTGTACAG 
TTTCAGGTGA TGGCCCAGGA AGTGGGTCCG GCTACTAATC TCACAGCCGT TGTAAACGTC 
ACCGTCTACA TCAATGACGT TAATGATAAT GCACCAGTCT TTGACCAGCC CTCCTACACA 
GTGGAGCTTC CCGAGAATAT GACAGTGGGA ACTAAGGTGG TTCAAGTGCA TGCCACTGAC 
TTAGACTCGG GATTGGGTGG CAAAGTACGA TATACTGCCA TTCTGGGGTA TCTAAATACA 
TCTGTTAACC TCGATGCAGA GAATGGATTA ATCACAATAT CGACAAATAA TCATGGCTAT 
GATCGCGAGG TAATGCCGGA ATATCACCTG TACGTAGAGG CTCGGGACAT GGATGGTGAA 
GGTAATCGAG CACAAGTCCC TCTAATCATT AAGCTAATAG ATGTTAACGA TGAGACGCCA 
ATATTTGACA AGGATCTGTA TGAGTTTATT TTGACGCCGG ATCTCTTGGG CTTCACCACC 
ACGGCCGTAA TTAATGCGGA AGACAAAGAT GCAACGGCAC CCAACAACGA AGTGCGGTAC 
GAACTGATCA GTGGCAACTA TGAAAACAAA TTTAAGCTGG ACAAGGTCAC CGGGGAATTG 
ACAATTCAGG ATAAGATTCA CCTGCGGTCC ATGACGGACG CCAGTGAACG AAGACATCGG 
GATCCAAGTA CATCGTCCAG TTCTGACGAT GACAACGAAG TGTTTATTTT AACTGCGCGT 
GCTTACGACC TAGGAGTGCC TGTGCGATTT TCGACAACAA CAATTCGCAT CTACCCACCT 
GAAAGTCGGA AGCGCATGGT GACTTTTGTG GTACCTGGTC ACAACCCAGA TAAGGCTAAA 
ACTGAAGAAA CGCTGTCTGC CATAACCGGC GGAAAGGTGT TAATTCACTC AATACGACCG 
CTGCGACCTG ATGAGCCAGG TGCCAAAGAC ATTCCTGTCA ATAATCCAGG TATCAAGGAC 
CGAAGTGTCG TCACCGCTAC GGTGCTATAT GGCAGCACGT CATTGGTTGA CATATCGGAT 
ATACAGCAGC GTCTGTCCCA GCACAATAAT TCCTATGCAA TTATGCCGCA AGACGACGCA 
TTACAGACGA ACAATCTAAC ACCACTGCAG ACTCAATATA AGGCTGAAAA TAAGGTGCTG 
TTCTGGCTGC TTATACTTCT GGCCACACTG GTCGCATTTA CCGTCCTCAT CCTACTTCTT 
TGCTGCATAT GCTCGTGGTG CCCTCTGTAC GGTGCAGCAA CCAAAAGAAT AGTTAATATC 
AGTAAAGCTG AAGACGATGT GCATCTAGTG CATAGGGAAA TGGCAAATGG AAAACATACA 
AAATCTGTTC AGGTCGCAGA GTGGATGGGA AGACGCGATG CCTGGTCAGC GGAGAAACCC 
CCGAATACCA GAACGAAACC TACCCGATGG GAGTTCCATG ACGGTCGTGA ACATCCTAGC 
GAAAACGTAG GGAGATTTCA GGACACCCGA GAGAAACATC ATGTACAATC AGCTGAGGAG 
CAACAGGGAC GCATTAGAAA ACAACGTACA GCCAAAGACG ATCTTCATCT TAGTTTTCAT 
AACTCTAGAA CTAATCTTAT TAATGACCGC GACGTATACA TGGAAGACTT AGTTGAAAAT 
CGAGATATAG TGGATGACAT TACCCGAAAC CGAGAAATTA AACAATTGGA CTATGCGCAA 
AGACAGCGCT GTGAAACCGA AGCCCACCAA ATCGACGACG ACTCTATGCG TAGACACGAA 
ATAGATCGCG GTAGCGACAT GGACTTCCCC GCTGCCCAAA ACAGTATGAA GAGCAAACGC 
GAACTGTTTA TAAAAGAAGG GAACGTGGAA ATTCTTCAAC TGATGACCAG AGATAAAATT 
CACGATGGTG CTGCACTAGA CGATGACAAC ATATATGTAA ACGTGCCACT AAAGCCCTTA 
GGAAACCTGT CACATCCGCA GCTTCTAATG GTGGACAATA CGGGTAAAGA GATTCTTATG 
CGGAGATTTA TTGAAGAGCA ACCAGATGGC AAGCAGATAA TTCGAGAGCA CTATCAAATA 
GTACCAGGAG CAACCTACAT TCAATCCATG CCGAATGAAG TACAACAAGC CTCAAATTTA 
AAAGTCGATA CGTATCCATT AGTCAAATCA GGGCCCAACA GTATTGTTTA TTCTCAAATG 
GAACCGGAAG TAAAGGTCAT ACATACACAG CCAGAGCATG CTGTTGAAGC TAGTTCAAAG 
ATGCGGCCTG CAATATCGAA CCACTCTCTT ACCCAGGAGC TGGAGCAGTC TCTTAAGCAA 
CAGAATTCCC TGCTGCGACA GATACTGCTC GAAAAGGACA AATTGGAGTC CGCCTATACA 
CAGGAAGTGG CATTAGAAAC ACAGAGTCTG CCTGGTCAGT CGATGGCTAT AGGTACGCAG 
ACTGACTGTG ATGCCGGCAC GCAAACTGAT ACAGTGGCAA GCCATATCGC GCACACATCC 
GTCAGTTCAC AAGCACATAT GAAAACCAAG TCGTCGCATC GTCGTGCTCG AAGCGAAAAT 
GACGAGTCCA TGTCTGAGGA TGGCTATGAA TATGTGCGGT TTATTCCACC AAATTGCCCT 
GAAGGTACGT TCTGGATCAA GCGTCGAAGG ACGAAAAAGC GCCGAAAACA GCCACGTAAA 
AGGATCGTGA TGGTGGAGGA GGTAAAACGT AAAATCCGCA CACCCATTAA AGAGGAAGAA 
GAGCTGCACG AGCGGAAGAG TCGAGTCCCA CCAAAGAAAC CAATGCGGGA GACCAAGACA 
AGTATACTGC GGAAACAGCT AAGCGACGAA AGCCGCAAAA CTGACGCCAC TTCCAATGGA 
GAATCGAAGG CGAGCGGGCA TCTGAAATCA GATGTACTTA TAGAGGTAAC GGACTCATTC 
GACGAAAATA AAAGTACCAG AAACCACAGC ACACGAAAGA TTCTAGTTGA TAAATATTAC 
AAACATTCCG ATGGGGAATT CGACGATGGC GAATTCGACG AAGGCGACGA TACAGAGTAT 
TCTTTTGACT CCGATGGAGA TGAAATCGTA ATCAGAACAA GCGCTGTAGT ACCTTCACGA 
GGGCAAAAAA GCGATCAATT TGTAATGGAA CCAAAAGAGA AGCGAAACTT ATCATTTTCT 
CCAGAAAATA TGGGAGGCAT AAAGCAGCAA AAGTATCCCC GTACACAGAC ACCAGAGGAG 
GCAGTCCAGG AGGCACAATT AAATTCAATT CCGAGATTAT CAAGAAGAGA CAGCAAGAAG 
CGGGACAATA ATCCCAGAAG ACAAACCTCA TCGGAGCCCC CGCACAGCCG ACTATCCGTT 
TCTAAGTTCG ATTCGATAGC TATTGAAGAC GATAATACAC CCCCAGCAGC CACATCATTG 
ATTGATCGAA GCTATCAAAG TGAAGCCGAG TTAGGAAATC TTGAGGGGGC AAGAGAACGC 
AATGTGCCCA AGTATATGGA GTGGTACTAC AATAAAAAAA AATCGTCTCT AAGCGGTCGT 
ACCTCGACGG AGTCATCGAA ATCTCAACCC ACGATCAAAA AAAAGGGTGG TTCATCAGAA 
AAACGCGTTA CAAAGACGCG CATCGCCGTC CAGAACCAAG AAAGTGATTT CCATGATGAT 
GGTGGAAAAT ATAAACCTGA GCCGGCTCCA CGCAGATCCG CGCCGAAAGG CAGTCGTTTG 
CTTAAGGAGG ACAGAGCTTT AAATAAGCAG CATAGACCAA AAAAAGAGAC TTACACAAAT 
CATCCACTAC TGCAGCACTC TGAACATAGG TTTGAGCGTG AAAGTGCAAT AGAAGTTCCA 
GCACAGCCTA CCAAACTGCC CCACTACATG TACCCAGAAA CTCCTCCGTA TGCAGCAACA 
GCTTCAGACA AGGAGCTCTC AGCTCAAAGA CAGCAGCCTA AGCCGTCCCC GATTAAAGAA 
AACGAAGTTA AAGTGACGAA CTCGAAAATA AATCTTCTTA AACAAGGTCG TAGCCTCGCA 
CATCAGACAT CATCGCATGC ACAAAAACAG TTAAATGCTT CGACGCTCGA GGATGACCAT 
GATTCAGGCA TAGCTATGAA CTCCCTTTTG AACAGCCTGG GTCGTCGCAA TCCGATTGCC 
GAAAAGAAAA GCGTATTCTC CATTGCCTAC GACGATGTTA GTCGGGTCAA AAAGATTCCC 
TCAGGTGGCG AGTCGCCACA ATACTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA26445 ( XM_015182128.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015037614.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA26445), mRNA.

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CloneID	ODe20428
Clone ID Related Accession (Same CDS sequence)	XM_015182128.1
Accession Version	XM_015182128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCGAT GGATAACACA TATTGCAGCT GTTCTTGAAC ATTCAACAGA AATGGCTTCC 
AATAGTAGCA GTCAATCTGT AAAGAACAAA ACAGTCATAC CGCCACGCCA CATGGGAAAG 
GAACAGAACG GCTTAACTTC AAATGCGGGA CCGATACTCG AAAAGGAATG CTACTACTAC 
ACCCCAACTA CCTCCCAGCA ACATCAACAG ATTCAACAAG GCTGGCAATC TCTATGGGCT 
TTTTTTGGGC CCTGGCTGAG TGCAATGACC GCCTATCGAT TGCTGACAAT CAGCCTCCTA 
ATTGGTATTC TGTGTCCACA TCACATTCAA GGCGCGGATC CTAAATTCGA TCCTTCGACG 
CGCATGCGAC TGGTACTGGT GCCGGCGGAC GCGCAAGTAA ACTCAGTTAT CTACAGATTG 
CGTGCCACTG ACGAGGAGTT CGACTATCCA TTGACCTTTG AGTTTGTGGG TGATGCATTT 
TCGTCCACCG TCAAAGTGGA TTCGCTTCCA TGCACCAAGT ACAATTCGGT TTGCCAAGCA 
AACATCGTGC TTCAGCGTCG CTTGGAGCCT GGCCGCTATT ACGACTTCCA GGTTTCAGTG 
AAGGACTCAA AAGGTGGCAT GGCCACTCAA TTGTGCTCAA TTACAGCAAC CAATTTTACC 
ACACCTCATG ACCTCATATT TCCACACAAG CCCGGTATTA TAATGATACC TGAGGATGCT 
AAACGCGGCA CGGAATTAAA TTACGTAATT GCGCGAAAGA ATCCACTGTT TCAAAAACCA 
GTCTACCTCG AGTTATGGGG TTCTCCGCTT TTTGCGATAA GACAAAAAAT TGTATCGTCC 
GAAACAACAG AAGGTACAGT TTTTCTTTTG GGACCATTGG ACTTCGAAAA GCAAGCCATG 
TACCATCTAA CTATACTTGC CAATGATGCT TACGCTGAAC CTGGCCAAGA TAGTCGCAAC 
ATCGCTGGCA TGGAAATTGT AGTCATCGTC CAGGATGTTC AAGACCAACC TCCTGTTTTC 
ACTTCGGCGC CGCCAGTTAC CAAACTGCCT ACTGGCATTC TACCAGGCGA TAAGATATTG 
CAAGTACATG CGGAGGATGG CGACAAAGGA AACCCGCGGG AGATTCGATT TGGATTGGTC 
TCGGAGAACA ACCCATTTAC TTCGTTTTTT GACATCAATG ACACAAGCGG TGAAATATTT 
TTAATGCGAC CTCTTGAAGA TATTGCTTTT ATAACGCATG TGGGTGATCC AGTGCTGCTT 
ACGGTAATTG CGGAAGAAGT TAAAGTTGGT CGTGACGAAC CGCCAGCATT GGCATCTACT 
GTGCAATTGG CGTTCTTTCT GCCAGATCGT ACCAATTCAC CGCCATACTT CGAAAACGAT 
CACTACGTTT CGAGGGTAGA CGAAAATGCA CCGCAAGGCA CAGCACTAAT ATTCGTTGAC 
CCATATGTAC CGCGCATCTA TGACGACGAC ACTGGAAAAA ATGGAGTTTT CTCGCTGACG 
TTGCTAAACA ATAATGGAAC ATTTGAGATA ACACCAAATG TAGCAGAGCG CAGTGCATCG 
TTTCTAGTGC GCGTACGAGA TAACACACTG TTAGACTACG AAATGCGACA CTCTGTACAG 
TTTCAGGTGA TGGCCCAGGA AGTGGGTCCG GCTACTAATC TCACAGCCGT TGTAAACGTC 
ACCGTCTACA TCAATGACGT TAATGATAAT GCACCAGTCT TTGACCAGCC CTCCTACACA 
GTGGAGCTTC CCGAGAATAT GACAGTGGGA ACTAAGGTGG TTCAAGTGCA TGCCACTGAC 
TTAGACTCGG GATTGGGTGG CAAAGTACGA TATACTGCCA TTCTGGGGTA TCTAAATACA 
TCTGTTAACC TCGATGCAGA GAATGGATTA ATCACAATAT CGACAAATAA TCATGGCTAT 
GATCGCGAGG TAATGCCGGA ATATCACCTG TACGTAGAGG CTCGGGACAT GGATGGTGAA 
GGTAATCGAG CACAAGTCCC TCTAATCATT AAGCTAATAG ATGTTAACGA TGAGACGCCA 
ATATTTGACA AGGATCTGTA TGAGTTTATT TTGACGCCGG ATCTCTTGGG CTTCACCACC 
ACGGCCGTAA TTAATGCGGA AGACAAAGAT GCAACGGCAC CCAACAACGA AGTGCGGTAC 
GAACTGATCA GTGGCAACTA TGAAAACAAA TTTAAGCTGG ACAAGGTCAC CGGGGAATTG 
ACAATTCAGG ATAAGATTCA CCTGCGGTCC ATGACGGACG CCAGTGAACG AAGACATCGG 
GATCCAAGTA CATCGTCCAG TTCTGACGAT GACAACGAAG TGTTTATTTT AACTGCGCGT 
GCTTACGACC TAGGAGTGCC TGTGCGATTT TCGACAACAA CAATTCGCAT CTACCCACCT 
GAAAGTCGGA AGCGCATGGT GACTTTTGTG GTACCTGGTC ACAACCCAGA TAAGGCTAAA 
ACTGAAGAAA CGCTGTCTGC CATAACCGGC GGAAAGGTGT TAATTCACTC AATACGACCG 
CTGCGACCTG ATGAGCCAGG TGCCAAAGAC ATTCCTGTCA ATAATCCAGG TATCAAGGAC 
CGAAGTGTCG TCACCGCTAC GGTGCTATAT GGCAGCACGT CATTGGTTGA CATATCGGAT 
ATACAGCAGC GTCTGTCCCA GCACAATAAT TCCTATGCAA TTATGCCGCA AGACGACGCA 
TTACAGACGA ACACTCAATA TAAGGCTGAA AATAAGGTGC TGTTCTGGCT GCTTATACTT 
CTGGCCACAC TGGTCGCATT TACCGTCCTC ATCCTACTTC TTTGCTGCAT ATGCTCGTGG 
TGCCCTCTGT ACGGTGCAGC AACCAAAAGA ATAGTTAATA TCAGTAAAGC TGAAGACGAT 
GTGCATCTAG TGCATAGGGA AATGGCAAAT GGAAAACATA CAAAATCTGT TCAGGTCGCA 
GAGTGGATGG GAAGACGCGA TGCCTGGTCA GCGGAGAAAC CCCCGAATAC CAGAACGAAA 
CCTACCCGAT GGGAGTTCCA TGACGGTCGT GAACATCCTA GCGAAAACGT AGGGAGATTT 
CAGGACACCC GAGAGAAACA TCATGTACAA TCAGCTGAGG AGCAACAGGG ACGCATTAGA 
AAACAACGTA CAGCCAAAGA CGATCTTCAT CTTAGTTTTC ATAACTCTAG AACTAATCTT 
ATTAATGACC GCGACGTATA CATGGAAGAC TTAGTTGAAA ATCGAGATAT AGTGGATGAC 
ATTACCCGAA ACCGAGAAAT TAAACAATTG GACTATGCGC AAAGACAGCG CTGTGAAACC 
GAAGCCCACC AAATCGACGA CGACTCTATG CGTAGACACG AAATAGATCG CGGTAGCGAC 
ATGGACTTCC CCGCTGCCCA AAACAGTATG AAGAGCAAAC GCGAACTGTT TATAAAAGAA 
GGGAACGTGG AAATTCTTCA ACTGATGACC AGAGATAAAA TTCACGATGG TGCTGCACTA 
GACGATGACA ACATATATGT AAACGTGCCA CTAAAGCCCT TAGGAAACCT GTCACATCCG 
CAGCTTCTAA TGGTGGACAA TACGGGTAAA GAGATTCTTA TGCGGAGATT TATTGAAGAG 
CAACCAGATG GCAAGCAGAT AATTCGAGAG CACTATCAAA TAGTACCAGG AGCAACCTAC 
ATTCAATCCA TGCCGAATGA AGTACAACAA GCCTCAAATT TAAAAGTCGA TACGTATCCA 
TTAGTCAAAT CAGGGCCCAA CAGTATTGTT TATTCTCAAA TGGAACCGGA AGTAAAGGTC 
ATACATACAC AGCCAGAGCA TGCTGTTGAA GCTAGTTCAA AGATGCGGCC TGCAATATCG 
AACCACTCTC TTACCCAGGA GCTGGAGCAG TCTCTTAAGC AACAGAATTC CCTGCTGCGA 
CAGATACTGC TCGAAAAGGA CAAATTGGAG TCCGCCTATA CACAGGAAGT GGCATTAGAA 
ACACAGAGTC TGCCTGGTCA GTCGATGGCT ATAGGTACGC AGACTGACTG TGATGCCGGC 
ACGCAAACTG ATACAGTGGC AAGCCATATC GCGCACACAT CCGTCAGTTC ACAAGCACAT 
ATGAAAACCA AGTCGTCGCA TCGTCGTGCT CGAAGCGAAA ATGACGAGTC CATGTCTGAG 
GATGGCTATG AATATGTGCG GTTTATTCCA CCAAATTGCC CTGAAGGTAC GTTCTGGATC 
AAGCGTCGAA GGACGAAAAA GCGCCGAAAA CAGCCACGTA AAAGGATCGT GATGGTGGAG 
GAGGTAAAAC GTAAAATCCG CACACCCATT AAAGAGGAAG AAGAGCTGCA CGAGCGGAAG 
AGTCGAGTCC CACCAAAGAA ACCAATGCGG GAGACCAAGA CAAGTATACT GCGGAAACAG 
CTAAGCGACG AAAGCCGCAA AACTGACGCC ACTTCCAATG GAGAATCGAA GGCGAGCGGG 
CATCTGAAAT CAGATGTACT TATAGAGGTA ACGGACTCAT TCGACGAAAA TAAAAGTACC 
AGAAACCACA GCACACGAAA GATTCTAGTT GATAAATATT ACAAACATTC CGATGGGGAA 
TTCGACGATG GCGAATTCGA CGAAGGCGAC GATACAGAGT ATTCTTTTGA CTCCGATGGA 
GATGAAATCG TAATCAGAAC AAGCGCTGTA GTACCTTCAC GAGGGCAAAA AAGCGATCAA 
TTTGTAATGG AACCAAAAGA GAAGCGAAAC TTATCATTTT CTCCAGAAAA TATGGGAGGC 
ATAAAGCAGC AAAAGTATCC CCGTACACAG ACACCAGAGG AGGCAGTCCA GGAGGCACAA 
TTAAATTCAA TTCCGAGATT ATCAAGAAGA GACAGCAAGA AGCGGGACAA TAATCCCAGA 
AGACAAACCT CATCGGAGCC CCCGCACAGC CGACTATCCG TTTCTAAGTT CGATTCGATA 
GCTATTGAAG ACGATAATAC ACCCCCAGCA GCCACATCAT TGATTGATCG AAGCTATCAA 
AGTGAAGCCG AGTTAGGAAA TCTTGAGGGG GCAAGAGAAC GCAATGTGCC CAAGTATATG 
GAGTGGTACT ACAATAAAAA AAAATCGTCT CTAAGCGGTC GTACCTCGAC GGAGTCATCG 
AAATCTCAAC CCACGATCAA AAAAAAGGGT GGTTCATCAG AAAAACGCGT TACAAAGACG 
CGCATCGCCG TCCAGAACCA AGAAAGTGAT TTCCATGATG ATGGTGGAAA ATATAAACCT 
GAGCCGGCTC CACGCAGATC CGCGCCGAAA GGCAGTCGTT TGCTTAAGGA GGACAGAGCT 
TTAAATAAGC AGCATAGACC AAAAAAAGAG ACTTACACAA ATCATCCACT ACTGCAGCAC 
TCTGAACATA GGTTTGAGCG TGAAAGTGCA ATAGAAGTTC CAGCACAGCC TACCAAACTG 
CCCCACTACA TGTACCCAGA AACTCCTCCG TATGCAGCAA CAGCTTCAGA CAAGGAGCTC 
TCAGCTCAAA GACAGCAGCC TAAGCCGTCC CCGATTAAAG AAAACGAAGT TAAAGTGACG 
AACTCGAAAA TAAATCTTCT TAAACAAGGT CGTAGCCTCG CACATCAGAC ATCATCGCAT 
GCACAAAAAC AGTTAAATGC TTCGACGCTC GAGGATGACC ATGATTCAGG CATAGCTATG 
AACTCCCTTT TGAACAGCCT GGGTCGTCGC AATCCGATTG CCGAAAAGAA AAGCGTATTC 
TCCATTGCCT ACGACGATGT TAGTCGGGTC AAAAAGATTC CCTCAGGTGG CGAGTCGCCA 
CAATACTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015037614.1
CDS	421..6192
Translation	MFRWITHIAAVLEHSTEMASNSSSQSVKNKTVIPPRHMGKEQNGLTSNAGPILEKECYYYTPTTSQQHQQIQQGWQSLWAFFGPWLSAMTAYRLLTISLLIGILCPHHIQGADPKFDPSTRMRLVLVPADAQVNSVIYRLRATDEEFDYPLTFEFVGDAFSSTVKVDSLPCTKYNSVCQANIVLQRRLEPGRYYDFQVSVKDSKGGMATQLCSITATNFTTPHDLIFPHKPGIIMIPEDAKRGTELNYVIARKNPLFQKPVYLELWGSPLFAIRQKIVSSETTEGTVFLLGPLDFEKQAMYHLTILANDAYAEPGQDSRNIAGMEIVVIVQDVQDQPPVFTSAPPVTKLPTGILPGDKILQVHAEDGDKGNPREIRFGLVSENNPFTSFFDINDTSGEIFLMRPLEDIAFITHVGDPVLLTVIAEEVKVGRDEPPALASTVQLAFFLPDRTNSPPYFENDHYVSRVDENAPQGTALIFVDPYVPRIYDDDTGKNGVFSLTLLNNNGTFEITPNVAERSASFLVRVRDNTLLDYEMRHSVQFQVMAQEVGPATNLTAVVNVTVYINDVNDNAPVFDQPSYTVELPENMTVGTKVVQVHATDLDSGLGGKVRYTAILGYLNTSVNLDAENGLITISTNNHGYDREVMPEYHLYVEARDMDGEGNRAQVPLIIKLIDVNDETPIFDKDLYEFILTPDLLGFTTTAVINAEDKDATAPNNEVRYELISGNYENKFKLDKVTGELTIQDKIHLRSMTDASERRHRDPSTSSSSDDDNEVFILTARAYDLGVPVRFSTTTIRIYPPESRKRMVTFVVPGHNPDKAKTEETLSAITGGKVLIHSIRPLRPDEPGAKDIPVNNPGIKDRSVVTATVLYGSTSLVDISDIQQRLSQHNNSYAIMPQDDALQTNTQYKAENKVLFWLLILLATLVAFTVLILLLCCICSWCPLYGAATKRIVNISKAEDDVHLVHREMANGKHTKSVQVAEWMGRRDAWSAEKPPNTRTKPTRWEFHDGREHPSENVGRFQDTREKHHVQSAEEQQGRIRKQRTAKDDLHLSFHNSRTNLINDRDVYMEDLVENRDIVDDITRNREIKQLDYAQRQRCETEAHQIDDDSMRRHEIDRGSDMDFPAAQNSMKSKRELFIKEGNVEILQLMTRDKIHDGAALDDDNIYVNVPLKPLGNLSHPQLLMVDNTGKEILMRRFIEEQPDGKQIIREHYQIVPGATYIQSMPNEVQQASNLKVDTYPLVKSGPNSIVYSQMEPEVKVIHTQPEHAVEASSKMRPAISNHSLTQELEQSLKQQNSLLRQILLEKDKLESAYTQEVALETQSLPGQSMAIGTQTDCDAGTQTDTVASHIAHTSVSSQAHMKTKSSHRRARSENDESMSEDGYEYVRFIPPNCPEGTFWIKRRRTKKRRKQPRKRIVMVEEVKRKIRTPIKEEEELHERKSRVPPKKPMRETKTSILRKQLSDESRKTDATSNGESKASGHLKSDVLIEVTDSFDENKSTRNHSTRKILVDKYYKHSDGEFDDGEFDEGDDTEYSFDSDGDEIVIRTSAVVPSRGQKSDQFVMEPKEKRNLSFSPENMGGIKQQKYPRTQTPEEAVQEAQLNSIPRLSRRDSKKRDNNPRRQTSSEPPHSRLSVSKFDSIAIEDDNTPPAATSLIDRSYQSEAELGNLEGARERNVPKYMEWYYNKKKSSLSGRTSTESSKSQPTIKKKGGSSEKRVTKTRIAVQNQESDFHDDGGKYKPEPAPRRSAPKGSRLLKEDRALNKQHRPKKETYTNHPLLQHSEHRFERESAIEVPAQPTKLPHYMYPETPPYAATASDKELSAQRQQPKPSPIKENEVKVTNSKINLLKQGRSLAHQTSSHAQKQLNASTLEDDHDSGIAMNSLLNSLGRRNPIAEKKSVFSIAYDDVSRVKKIPSGGESPQYS

Target ORF information:

RefSeq Version	XM_015182128.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA26445), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015182128.1

ORF Insert Sequence:

ATGTTTCGAT GGATAACACA TATTGCAGCT GTTCTTGAAC ATTCAACAGA AATGGCTTCC 
AATAGTAGCA GTCAATCTGT AAAGAACAAA ACAGTCATAC CGCCACGCCA CATGGGAAAG 
GAACAGAACG GCTTAACTTC AAATGCGGGA CCGATACTCG AAAAGGAATG CTACTACTAC 
ACCCCAACTA CCTCCCAGCA ACATCAACAG ATTCAACAAG GCTGGCAATC TCTATGGGCT 
TTTTTTGGGC CCTGGCTGAG TGCAATGACC GCCTATCGAT TGCTGACAAT CAGCCTCCTA 
ATTGGTATTC TGTGTCCACA TCACATTCAA GGCGCGGATC CTAAATTCGA TCCTTCGACG 
CGCATGCGAC TGGTACTGGT GCCGGCGGAC GCGCAAGTAA ACTCAGTTAT CTACAGATTG 
CGTGCCACTG ACGAGGAGTT CGACTATCCA TTGACCTTTG AGTTTGTGGG TGATGCATTT 
TCGTCCACCG TCAAAGTGGA TTCGCTTCCA TGCACCAAGT ACAATTCGGT TTGCCAAGCA 
AACATCGTGC TTCAGCGTCG CTTGGAGCCT GGCCGCTATT ACGACTTCCA GGTTTCAGTG 
AAGGACTCAA AAGGTGGCAT GGCCACTCAA TTGTGCTCAA TTACAGCAAC CAATTTTACC 
ACACCTCATG ACCTCATATT TCCACACAAG CCCGGTATTA TAATGATACC TGAGGATGCT 
AAACGCGGCA CGGAATTAAA TTACGTAATT GCGCGAAAGA ATCCACTGTT TCAAAAACCA 
GTCTACCTCG AGTTATGGGG TTCTCCGCTT TTTGCGATAA GACAAAAAAT TGTATCGTCC 
GAAACAACAG AAGGTACAGT TTTTCTTTTG GGACCATTGG ACTTCGAAAA GCAAGCCATG 
TACCATCTAA CTATACTTGC CAATGATGCT TACGCTGAAC CTGGCCAAGA TAGTCGCAAC 
ATCGCTGGCA TGGAAATTGT AGTCATCGTC CAGGATGTTC AAGACCAACC TCCTGTTTTC 
ACTTCGGCGC CGCCAGTTAC CAAACTGCCT ACTGGCATTC TACCAGGCGA TAAGATATTG 
CAAGTACATG CGGAGGATGG CGACAAAGGA AACCCGCGGG AGATTCGATT TGGATTGGTC 
TCGGAGAACA ACCCATTTAC TTCGTTTTTT GACATCAATG ACACAAGCGG TGAAATATTT 
TTAATGCGAC CTCTTGAAGA TATTGCTTTT ATAACGCATG TGGGTGATCC AGTGCTGCTT 
ACGGTAATTG CGGAAGAAGT TAAAGTTGGT CGTGACGAAC CGCCAGCATT GGCATCTACT 
GTGCAATTGG CGTTCTTTCT GCCAGATCGT ACCAATTCAC CGCCATACTT CGAAAACGAT 
CACTACGTTT CGAGGGTAGA CGAAAATGCA CCGCAAGGCA CAGCACTAAT ATTCGTTGAC 
CCATATGTAC CGCGCATCTA TGACGACGAC ACTGGAAAAA ATGGAGTTTT CTCGCTGACG 
TTGCTAAACA ATAATGGAAC ATTTGAGATA ACACCAAATG TAGCAGAGCG CAGTGCATCG 
TTTCTAGTGC GCGTACGAGA TAACACACTG TTAGACTACG AAATGCGACA CTCTGTACAG 
TTTCAGGTGA TGGCCCAGGA AGTGGGTCCG GCTACTAATC TCACAGCCGT TGTAAACGTC 
ACCGTCTACA TCAATGACGT TAATGATAAT GCACCAGTCT TTGACCAGCC CTCCTACACA 
GTGGAGCTTC CCGAGAATAT GACAGTGGGA ACTAAGGTGG TTCAAGTGCA TGCCACTGAC 
TTAGACTCGG GATTGGGTGG CAAAGTACGA TATACTGCCA TTCTGGGGTA TCTAAATACA 
TCTGTTAACC TCGATGCAGA GAATGGATTA ATCACAATAT CGACAAATAA TCATGGCTAT 
GATCGCGAGG TAATGCCGGA ATATCACCTG TACGTAGAGG CTCGGGACAT GGATGGTGAA 
GGTAATCGAG CACAAGTCCC TCTAATCATT AAGCTAATAG ATGTTAACGA TGAGACGCCA 
ATATTTGACA AGGATCTGTA TGAGTTTATT TTGACGCCGG ATCTCTTGGG CTTCACCACC 
ACGGCCGTAA TTAATGCGGA AGACAAAGAT GCAACGGCAC CCAACAACGA AGTGCGGTAC 
GAACTGATCA GTGGCAACTA TGAAAACAAA TTTAAGCTGG ACAAGGTCAC CGGGGAATTG 
ACAATTCAGG ATAAGATTCA CCTGCGGTCC ATGACGGACG CCAGTGAACG AAGACATCGG 
GATCCAAGTA CATCGTCCAG TTCTGACGAT GACAACGAAG TGTTTATTTT AACTGCGCGT 
GCTTACGACC TAGGAGTGCC TGTGCGATTT TCGACAACAA CAATTCGCAT CTACCCACCT 
GAAAGTCGGA AGCGCATGGT GACTTTTGTG GTACCTGGTC ACAACCCAGA TAAGGCTAAA 
ACTGAAGAAA CGCTGTCTGC CATAACCGGC GGAAAGGTGT TAATTCACTC AATACGACCG 
CTGCGACCTG ATGAGCCAGG TGCCAAAGAC ATTCCTGTCA ATAATCCAGG TATCAAGGAC 
CGAAGTGTCG TCACCGCTAC GGTGCTATAT GGCAGCACGT CATTGGTTGA CATATCGGAT 
ATACAGCAGC GTCTGTCCCA GCACAATAAT TCCTATGCAA TTATGCCGCA AGACGACGCA 
TTACAGACGA ACACTCAATA TAAGGCTGAA AATAAGGTGC TGTTCTGGCT GCTTATACTT 
CTGGCCACAC TGGTCGCATT TACCGTCCTC ATCCTACTTC TTTGCTGCAT ATGCTCGTGG 
TGCCCTCTGT ACGGTGCAGC AACCAAAAGA ATAGTTAATA TCAGTAAAGC TGAAGACGAT 
GTGCATCTAG TGCATAGGGA AATGGCAAAT GGAAAACATA CAAAATCTGT TCAGGTCGCA 
GAGTGGATGG GAAGACGCGA TGCCTGGTCA GCGGAGAAAC CCCCGAATAC CAGAACGAAA 
CCTACCCGAT GGGAGTTCCA TGACGGTCGT GAACATCCTA GCGAAAACGT AGGGAGATTT 
CAGGACACCC GAGAGAAACA TCATGTACAA TCAGCTGAGG AGCAACAGGG ACGCATTAGA 
AAACAACGTA CAGCCAAAGA CGATCTTCAT CTTAGTTTTC ATAACTCTAG AACTAATCTT 
ATTAATGACC GCGACGTATA CATGGAAGAC TTAGTTGAAA ATCGAGATAT AGTGGATGAC 
ATTACCCGAA ACCGAGAAAT TAAACAATTG GACTATGCGC AAAGACAGCG CTGTGAAACC 
GAAGCCCACC AAATCGACGA CGACTCTATG CGTAGACACG AAATAGATCG CGGTAGCGAC 
ATGGACTTCC CCGCTGCCCA AAACAGTATG AAGAGCAAAC GCGAACTGTT TATAAAAGAA 
GGGAACGTGG AAATTCTTCA ACTGATGACC AGAGATAAAA TTCACGATGG TGCTGCACTA 
GACGATGACA ACATATATGT AAACGTGCCA CTAAAGCCCT TAGGAAACCT GTCACATCCG 
CAGCTTCTAA TGGTGGACAA TACGGGTAAA GAGATTCTTA TGCGGAGATT TATTGAAGAG 
CAACCAGATG GCAAGCAGAT AATTCGAGAG CACTATCAAA TAGTACCAGG AGCAACCTAC 
ATTCAATCCA TGCCGAATGA AGTACAACAA GCCTCAAATT TAAAAGTCGA TACGTATCCA 
TTAGTCAAAT CAGGGCCCAA CAGTATTGTT TATTCTCAAA TGGAACCGGA AGTAAAGGTC 
ATACATACAC AGCCAGAGCA TGCTGTTGAA GCTAGTTCAA AGATGCGGCC TGCAATATCG 
AACCACTCTC TTACCCAGGA GCTGGAGCAG TCTCTTAAGC AACAGAATTC CCTGCTGCGA 
CAGATACTGC TCGAAAAGGA CAAATTGGAG TCCGCCTATA CACAGGAAGT GGCATTAGAA 
ACACAGAGTC TGCCTGGTCA GTCGATGGCT ATAGGTACGC AGACTGACTG TGATGCCGGC 
ACGCAAACTG ATACAGTGGC AAGCCATATC GCGCACACAT CCGTCAGTTC ACAAGCACAT 
ATGAAAACCA AGTCGTCGCA TCGTCGTGCT CGAAGCGAAA ATGACGAGTC CATGTCTGAG 
GATGGCTATG AATATGTGCG GTTTATTCCA CCAAATTGCC CTGAAGGTAC GTTCTGGATC 
AAGCGTCGAA GGACGAAAAA GCGCCGAAAA CAGCCACGTA AAAGGATCGT GATGGTGGAG 
GAGGTAAAAC GTAAAATCCG CACACCCATT AAAGAGGAAG AAGAGCTGCA CGAGCGGAAG 
AGTCGAGTCC CACCAAAGAA ACCAATGCGG GAGACCAAGA CAAGTATACT GCGGAAACAG 
CTAAGCGACG AAAGCCGCAA AACTGACGCC ACTTCCAATG GAGAATCGAA GGCGAGCGGG 
CATCTGAAAT CAGATGTACT TATAGAGGTA ACGGACTCAT TCGACGAAAA TAAAAGTACC 
AGAAACCACA GCACACGAAA GATTCTAGTT GATAAATATT ACAAACATTC CGATGGGGAA 
TTCGACGATG GCGAATTCGA CGAAGGCGAC GATACAGAGT ATTCTTTTGA CTCCGATGGA 
GATGAAATCG TAATCAGAAC AAGCGCTGTA GTACCTTCAC GAGGGCAAAA AAGCGATCAA 
TTTGTAATGG AACCAAAAGA GAAGCGAAAC TTATCATTTT CTCCAGAAAA TATGGGAGGC 
ATAAAGCAGC AAAAGTATCC CCGTACACAG ACACCAGAGG AGGCAGTCCA GGAGGCACAA 
TTAAATTCAA TTCCGAGATT ATCAAGAAGA GACAGCAAGA AGCGGGACAA TAATCCCAGA 
AGACAAACCT CATCGGAGCC CCCGCACAGC CGACTATCCG TTTCTAAGTT CGATTCGATA 
GCTATTGAAG ACGATAATAC ACCCCCAGCA GCCACATCAT TGATTGATCG AAGCTATCAA 
AGTGAAGCCG AGTTAGGAAA TCTTGAGGGG GCAAGAGAAC GCAATGTGCC CAAGTATATG 
GAGTGGTACT ACAATAAAAA AAAATCGTCT CTAAGCGGTC GTACCTCGAC GGAGTCATCG 
AAATCTCAAC CCACGATCAA AAAAAAGGGT GGTTCATCAG AAAAACGCGT TACAAAGACG 
CGCATCGCCG TCCAGAACCA AGAAAGTGAT TTCCATGATG ATGGTGGAAA ATATAAACCT 
GAGCCGGCTC CACGCAGATC CGCGCCGAAA GGCAGTCGTT TGCTTAAGGA GGACAGAGCT 
TTAAATAAGC AGCATAGACC AAAAAAAGAG ACTTACACAA ATCATCCACT ACTGCAGCAC 
TCTGAACATA GGTTTGAGCG TGAAAGTGCA ATAGAAGTTC CAGCACAGCC TACCAAACTG 
CCCCACTACA TGTACCCAGA AACTCCTCCG TATGCAGCAA CAGCTTCAGA CAAGGAGCTC 
TCAGCTCAAA GACAGCAGCC TAAGCCGTCC CCGATTAAAG AAAACGAAGT TAAAGTGACG 
AACTCGAAAA TAAATCTTCT TAAACAAGGT CGTAGCCTCG CACATCAGAC ATCATCGCAT 
GCACAAAAAC AGTTAAATGC TTCGACGCTC GAGGATGACC ATGATTCAGG CATAGCTATG 
AACTCCCTTT TGAACAGCCT GGGTCGTCGC AATCCGATTG CCGAAAAGAA AAGCGTATTC 
TCCATTGCCT ACGACGATGT TAGTCGGGTC AAAAAGATTC CCTCAGGTGG CGAGTCGCCA 
CAATACTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA26445 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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