Dpse\GA10956 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA10956
Entrez Gene ID	4800428
Full Name	GA10956 gene product from transcript GA10956-RA
Synonyms	GA10956,dpse_GLEANR_4868
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001357669.4	XP_001357706.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA10956 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA10956 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe18993	XM_001357669.4 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA10956), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA10956 ( XM_001357669.4 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001357706.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA10956), mRNA.

Price & Availability↑

CloneID	ODe18993
Clone ID Related Accession (Same CDS sequence)	XM_001357669.4
Accession Version	XM_001357669.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001357669.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCTGA GTCGCAAGCG TCGCGCCAGC TCCATATCGA GTCGCCAGGA CGAAGATCCG 
CTGATGCTGG ACGACTCGAC GCCGGAGCAG TCGCCGGTGC AGCAGACAAC GACACAATCG 
GCGCGAAAAA AGCGCCGACT GGATCCGACC GAGCTCTGCC AGCAGTTGTA CGACTCCATA 
CGGAACATCA AGAAGGAGGA TGGCTCGATG CTGTGCGATA CGTTCATACG GGCGCCAAAG 
AGACGGCAGG AGCCCTCCTA CTACGACGTG GTGGTGAATC CCATCGACTT GCTGAAGGTA 
CAGCAGAAAC TTAAGACAGA CTCGTACGAC GATCTCGAAG ACCTGATGGC CGATCTGGAG 
CTGCTGATTG GCAACGCGAA GGCCTTCTAC AAGCCGGAGA GCGCCGAGTA CCGAGATGCC 
CACGCCCTGT GGCAGCACAT CCAGTCGCAG AGGCAGCGCA TAATGGAGGC CAATGGCATG 
GCGGAAGAGG AGCCGCGTGC CAAGCGCATT TCGCGCACTA TCCGCCGAAT GACAACCAGC 
ACGGAGCCGT GCGGTGATCT CGACGATGAA TTCAATCAGT ATGAGGAGCT CTTTGCCTCG 
GTAATGACAG CCACCGACCC GATCACCGAT AGGGCCATGT ACCGCATGTT CCAGCTGCTG 
CCTTCGAAGA AGATCTACCC GGACTATTAC GATGTGATTG AGCATCCGAT TGACCTGCGT 
CTGATTGCGA CCAAAATCCA AATGAATGCC TACTCCTCGC TGATGGAAAT GGAACGAGAT 
CTGCTGCAGA TGACTAAGAA TGCCTGCCTC TTCAACGAGC CGGGCTCGCA GATCTACAAG 
GATGCCAAGG CGCTGAAGCG CATTTTTACA CAAAGGCGTG GCGAGCTGGA AGCGGGCAAG 
GGGAAGCTGG CCAGGCGACT CAAGAGTTTG TCCAGTGCGG TCATAGCCGC ACTAAAAGAA 
AAGGTGGAGA GTTCCGATGA CGAGGAGACG AGTAAAAAGG GCGAAGGTCC CATGTGGGCC 
CTGTTCGATT ACCTTTATAA TGCACCTGGG ACATCGGAGC ATCCTGGCAT CACGGGGCCA 
CCGCTGGGCA ACTCCCTGTG GAAGCTGCCG GTGCGTCGCT TTCACCCGGA ATACTTTGAG 
ATCATCAAGC GGCCCATTTC CATGAGCCAG ATCCACACGA AGCTGAAGAA GGGCGACTAT 
GCGAATATAA GTGATATCAC CTCCGATCTG TATCTCATGC TGGACAACGC CAAGAAGGCG 
TTCGTGCCCA GCCATCGAAC CCACAAAGAT GCTCTCAAGA TGCTAAAAAT CATGAATGCC 
AAGCTGGTGG AGGAGTCCCT CGAGGAGACT GGAGACGAGC TGGAAGAGGA AGAGGGGGAC 
GATCTGCTGG AGCCCGAAAG CTTTATATCG AGCAACGCCC AGACGGAGAA GCGGAAGCCG 
GGCAGGCCGC GCATCAACTC CAATTCCAAC TCGAACACCT CGCACACGCC GAACAACTCG 
AATTCGCCCA AAACGAATCG CATTGCCATC AATGGGGCCA TCAAGAAGAA GATCCTTGCC 
ATCCAGAAGT GCCTCGTGGA GTACTTGGTG GGGAATCGTC GGCCCATCGA GCTGTTCATG 
GAGAAGCCGC CCCGCAAAGT CTATCCGGAC TACTACGACA TCATCCAGAA TCCCATCGAC 
ATGAGCACCA TCGATCACAA TATACGCACC GATCGCTATG CCACTGTGGA GGATGTCGTC 
TCCGACTACC GGCTGATGTT CTCCAACTGT CGGCAGTACA ACGAGGAGGG CTCTACCATC 
TACGAAGATG CCAACATTCT GGAGCGAGCC CTCAACGAGA AGCTCAAGGA GTTCCCGGGC 
CTCGTGGAGG TGAAAAAACC CCTTCAGAAG TACTCCAAGG TGGGGCGCAA GCTGAAGACG 
GCTCTGGTTA CCGACCGCCT CTGGCAGTTC TATGAGACAC TGCGCGAGTA TCAGGAGCCC 
AAGGGCAAGC GTCAGCTCTC GCTGATTTTC ACCAAGCTGC CGTCGAAGGG CGAGTATCCG 
GACTACTACG ACATCATCAA GGAGCCCATG GACATGGACC GCATTGCCCA GAAGCTGAAG 
CAGTCCGCCT ACGAGACCCT GGACGAGCTG GCCGCCGATT TCCTGCTGAT GCTGGAGAAC 
GCCTGCAAGT ACAACGAGCC CGACTCGCAG ATCTACAAGG ATGCTCTGGT GCTGCAGCAG 
CTGACGCTGC AGTTGAAGCA GCAGCTGCGT ATCGAGCGCG ACTCGCTGCC GGATGTCCCG 
CTGGCCGTGC AGGAGCTGTT CCTCACGCTG TTCACGACCC TGTACAATCA CCAGGATGAG 
GAGGGACGCT GCTACTCCGA CTCCTTGGCC GAGCTGGCCG AGTACGATGA GCTGGGCGAG 
GGGGCCAAGG TGCGCGGCAT TTCGCTGGAC TTGATCAAGC GAAGGCTGGA CAAGGGCGCC 
TACAAGCGAC TGGATATCTA CCAGGAGGAC ATCTTCTCCT GCCTGGAGCG TGCCCGCAAA 
CTGTCGCGCA CGGACTCGGA TATATTTCAG GACTCCATCG AGCTGCAGAC CTACTTCATA 
CGCAAGCGGG ATGAGCTGTG CAAGGACACG CTCAGCTCGC CGGCTCTGAG CTTTACGCTG 
GAGCGCCTGT TGGCCGATGT GGAGGTGATG CGACAGCAGA AGCTGCTGCA AGAGGAGCAG 
GAACAGGAGC AGGACAAAGA TGAGTTCAAT GCCGCCAGCA AGGGTGAGAG CATGACAATC 
AATCAGCAGG TCTACTCCCC CGGCGACTAT GTGTACGTGC AGATGCCGGA GAACAAGATA 
CCCTCGATCG CCTGCATCGA GAGGCTGTGG ACATCGCCCA GCAACGAGAA GCTGATGCAG 
GGCTCGATCT TCGTGCGGCC CCACGAGACG TATCACGTGA CGACGCGGAA GTTCCTCGAG 
AAGGAGGTGT TCAAGAGCAG CATCTCGCAG ACCATCTCCA TGGACAAGGT ACTGGGCATG 
TGCTATGTGA TGCACATCAA GGACTACATC AAGATGCGTC CCGACCGGCT GCAAGAGAAG 
GATGTGTTCG TGTGCGAGTC GCGCTACAAT CTGCAGGGAC GGTGCTTCAA GAAGCTCAAG 
AACTGGCCCC AGATACGCGA GGGCAGCTCC GTGAGGTTTG TGCCGCGCGA CCAGCCGCTG 
GAGCTGAAGC GCGTGATGTC CGTGTTCAAG GAGCGCATCG AGAAGCACAA GGGGGAGCTG 
GAGGAGCTCA AAATGCAGGA GACGATGGTG GAGAAGGAGA AGCCCAATGT GTCCTGCGAC 
CCGCCACCGA ATGCCGAGAA CAACAGTGTC TACTACCAGC AGTACAACAC CATCTGCAGC 
GGGGCCATCA AGACGGGCGA CTTCGTCTAC GTGGCCACCC AAACGGGCAA GCAGTCCGTG 
GCGCAGGTCC AGCAGATCTG GGAGCAGAAC GGTAAGTCAT ACTTCAAGGG TCCCTGGCTG 
CTGCCCCCCA GCGAAACGAC CCCCGCCCTG GGCAAGCAGT TCTTCCGCCA GGAGCTGCTC 
CTCACCACCG TCGAAGAGGT CAGTCCCGTG GTGGGTGTTG TGGGTCGCTG CGCCGTGCTG 
GAGTACGCGG AGTTCATCAA CTCCCGACCC ACAGAGATAG CCGAGAGCGA CGTCTACATC 
TGCGAGTCCG TCTACGACGA GCTGAAGAAG GCCGTGCGCA AACTGGTGGC CGGTAACATG 
CGCAAGTTCC AGCACAGCTC GGCCGTCACC GAGGATGAGA TCTTCTACTT CAAGACTCCC 
ATCAAGCCGG CCAAGGATGT GAAGAGCGAG ATCAACGATC TGAGCATGTT GGAGGACTCG 
ATGGATGGTG ATACACCGTC TCTGAGCTCG GACATAGCGG CCATGTCCTC GCCGGCGCCC 
TCTGTGAACT CCACGCCTCT GACCTCCAAA GTGAAGGCTG CCAAATCGGC CAAGAAATGC 
CTCACCGGCT ACATTCTCTA CTCCAGCGAA GTGCGAAAGG GCATTTGCCA AAGCAATCCA 
GAGGCCTCCT TTGGGGACAT ATCCCGAATG GTGGGCAACG AATGGAAGAA TCTACCGTCC 
AGCGTAAAGC AAAGTTGGGA GGATCGCGCC AGCAAACAGA ACGAGGAGAC GGCAGCCCTG 
CGCCGTGAGA TAGATGACAC GCAGAACAGT GCCAGTCCTT CGTCCCAGGT GAGTGCCACC 
CAGGAGTCCA CAGGTCCACC GCTCACCTTC GAGTGCCTGT GGGACAAGTG CGATTTCCAG 
TTCGAGGAAA TGCCGGACTG CACCGAACAC TGCCTCTCCG AAGCCACCGG CCACATACAG 
CGGCATCCGC CGTCGGGCGT GGAGATTGAG TACGTCTGCC TCTGGCGCAA CTGTTCTCGC 
ATCAAGAAGT CCACCCAGGG ATTCCCCAAC GTGGTGCGGC TCATCAAGCA CGTGCGCGAA 
GTGCATCTGA GCAAGTGCGG AAAGGTTTTG GGCATCACCG AGCGCAGCAA GAACTTTGTG 
CCGCGCCGGC AGAAGCACAT GCCCCAGATG GCTGCCACTG TGCCGCTGCC GCCCAACAAT 
GCCCATTCAC CGCGTGCCGC CAACACCGCT GAGGCAGCGC AGCTGCAACA GCAGGCGCCG 
CACACTCAGC AGATGATGCA GCAGCCGCAG CAAACGATTG TGCTCGGACC ACCGCCAGAG 
CCGATGTTTG TCACCGTGCC GCCGCGCACC ACTCGAGTCA TCCATTCGGA GGCCTACATC 
AAGTACATCG AGAGCCTCCA GACGGGCAGC CACCTCAACG TGGCCGCCTG CAACAACAAC 
TGGCGGCGAG CCCTCACACA CGTGACTCCC GCCCAAGTCG TGCCGAAGGT GCCACTGCCC 
GAACACTGGC TGGGACCCAA TGCCCGCGAT CAGGACAGCG TTGTGCAGGC CCTGTGCCAC 
CTACGCAACT TTATGCTAGA CGATGTGCTG CAGATTCGCC GCAGCTGCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001357706.2
CDS	347..5320
Translation	MMLSRKRRASSISSRQDEDPLMLDDSTPEQSPVQQTTTQSARKKRRLDPTELCQQLYDSIRNIKKEDGSMLCDTFIRAPKRRQEPSYYDVVVNPIDLLKVQQKLKTDSYDDLEDLMADLELLIGNAKAFYKPESAEYRDAHALWQHIQSQRQRIMEANGMAEEEPRAKRISRTIRRMTTSTEPCGDLDDEFNQYEELFASVMTATDPITDRAMYRMFQLLPSKKIYPDYYDVIEHPIDLRLIATKIQMNAYSSLMEMERDLLQMTKNACLFNEPGSQIYKDAKALKRIFTQRRGELEAGKGKLARRLKSLSSAVIAALKEKVESSDDEETSKKGEGPMWALFDYLYNAPGTSEHPGITGPPLGNSLWKLPVRRFHPEYFEIIKRPISMSQIHTKLKKGDYANISDITSDLYLMLDNAKKAFVPSHRTHKDALKMLKIMNAKLVEESLEETGDELEEEEGDDLLEPESFISSNAQTEKRKPGRPRINSNSNSNTSHTPNNSNSPKTNRIAINGAIKKKILAIQKCLVEYLVGNRRPIELFMEKPPRKVYPDYYDIIQNPIDMSTIDHNIRTDRYATVEDVVSDYRLMFSNCRQYNEEGSTIYEDANILERALNEKLKEFPGLVEVKKPLQKYSKVGRKLKTALVTDRLWQFYETLREYQEPKGKRQLSLIFTKLPSKGEYPDYYDIIKEPMDMDRIAQKLKQSAYETLDELAADFLLMLENACKYNEPDSQIYKDALVLQQLTLQLKQQLRIERDSLPDVPLAVQELFLTLFTTLYNHQDEEGRCYSDSLAELAEYDELGEGAKVRGISLDLIKRRLDKGAYKRLDIYQEDIFSCLERARKLSRTDSDIFQDSIELQTYFIRKRDELCKDTLSSPALSFTLERLLADVEVMRQQKLLQEEQEQEQDKDEFNAASKGESMTINQQVYSPGDYVYVQMPENKIPSIACIERLWTSPSNEKLMQGSIFVRPHETYHVTTRKFLEKEVFKSSISQTISMDKVLGMCYVMHIKDYIKMRPDRLQEKDVFVCESRYNLQGRCFKKLKNWPQIREGSSVRFVPRDQPLELKRVMSVFKERIEKHKGELEELKMQETMVEKEKPNVSCDPPPNAENNSVYYQQYNTICSGAIKTGDFVYVATQTGKQSVAQVQQIWEQNGKSYFKGPWLLPPSETTPALGKQFFRQELLLTTVEEVSPVVGVVGRCAVLEYAEFINSRPTEIAESDVYICESVYDELKKAVRKLVAGNMRKFQHSSAVTEDEIFYFKTPIKPAKDVKSEINDLSMLEDSMDGDTPSLSSDIAAMSSPAPSVNSTPLTSKVKAAKSAKKCLTGYILYSSEVRKGICQSNPEASFGDISRMVGNEWKNLPSSVKQSWEDRASKQNEETAALRREIDDTQNSASPSSQVSATQESTGPPLTFECLWDKCDFQFEEMPDCTEHCLSEATGHIQRHPPSGVEIEYVCLWRNCSRIKKSTQGFPNVVRLIKHVREVHLSKCGKVLGITERSKNFVPRRQKHMPQMAATVPLPPNNAHSPRAANTAEAAQLQQQAPHTQQMMQQPQQTIVLGPPPEPMFVTVPPRTTRVIHSEAYIKYIESLQTGSHLNVAACNNNWRRALTHVTPAQVVPKVPLPEHWLGPNARDQDSVVQALCHLRNFMLDDVLQIRRSCN

Target ORF information:

RefSeq Version	XM_001357669.4
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA10956), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001357669.4

ORF Insert Sequence:

ATGATGCTGA GTCGCAAGCG TCGCGCCAGC TCCATATCGA GTCGCCAGGA CGAAGATCCG 
CTGATGCTGG ACGACTCGAC GCCGGAGCAG TCGCCGGTGC AGCAGACAAC GACACAATCG 
GCGCGAAAAA AGCGCCGACT GGATCCGACC GAGCTCTGCC AGCAGTTGTA CGACTCCATA 
CGGAACATCA AGAAGGAGGA TGGCTCGATG CTGTGCGATA CGTTCATACG GGCGCCAAAG 
AGACGGCAGG AGCCCTCCTA CTACGACGTG GTGGTGAATC CCATCGACTT GCTGAAGGTA 
CAGCAGAAAC TTAAGACAGA CTCGTACGAC GATCTCGAAG ACCTGATGGC CGATCTGGAG 
CTGCTGATTG GCAACGCGAA GGCCTTCTAC AAGCCGGAGA GCGCCGAGTA CCGAGATGCC 
CACGCCCTGT GGCAGCACAT CCAGTCGCAG AGGCAGCGCA TAATGGAGGC CAATGGCATG 
GCGGAAGAGG AGCCGCGTGC CAAGCGCATT TCGCGCACTA TCCGCCGAAT GACAACCAGC 
ACGGAGCCGT GCGGTGATCT CGACGATGAA TTCAATCAGT ATGAGGAGCT CTTTGCCTCG 
GTAATGACAG CCACCGACCC GATCACCGAT AGGGCCATGT ACCGCATGTT CCAGCTGCTG 
CCTTCGAAGA AGATCTACCC GGACTATTAC GATGTGATTG AGCATCCGAT TGACCTGCGT 
CTGATTGCGA CCAAAATCCA AATGAATGCC TACTCCTCGC TGATGGAAAT GGAACGAGAT 
CTGCTGCAGA TGACTAAGAA TGCCTGCCTC TTCAACGAGC CGGGCTCGCA GATCTACAAG 
GATGCCAAGG CGCTGAAGCG CATTTTTACA CAAAGGCGTG GCGAGCTGGA AGCGGGCAAG 
GGGAAGCTGG CCAGGCGACT CAAGAGTTTG TCCAGTGCGG TCATAGCCGC ACTAAAAGAA 
AAGGTGGAGA GTTCCGATGA CGAGGAGACG AGTAAAAAGG GCGAAGGTCC CATGTGGGCC 
CTGTTCGATT ACCTTTATAA TGCACCTGGG ACATCGGAGC ATCCTGGCAT CACGGGGCCA 
CCGCTGGGCA ACTCCCTGTG GAAGCTGCCG GTGCGTCGCT TTCACCCGGA ATACTTTGAG 
ATCATCAAGC GGCCCATTTC CATGAGCCAG ATCCACACGA AGCTGAAGAA GGGCGACTAT 
GCGAATATAA GTGATATCAC CTCCGATCTG TATCTCATGC TGGACAACGC CAAGAAGGCG 
TTCGTGCCCA GCCATCGAAC CCACAAAGAT GCTCTCAAGA TGCTAAAAAT CATGAATGCC 
AAGCTGGTGG AGGAGTCCCT CGAGGAGACT GGAGACGAGC TGGAAGAGGA AGAGGGGGAC 
GATCTGCTGG AGCCCGAAAG CTTTATATCG AGCAACGCCC AGACGGAGAA GCGGAAGCCG 
GGCAGGCCGC GCATCAACTC CAATTCCAAC TCGAACACCT CGCACACGCC GAACAACTCG 
AATTCGCCCA AAACGAATCG CATTGCCATC AATGGGGCCA TCAAGAAGAA GATCCTTGCC 
ATCCAGAAGT GCCTCGTGGA GTACTTGGTG GGGAATCGTC GGCCCATCGA GCTGTTCATG 
GAGAAGCCGC CCCGCAAAGT CTATCCGGAC TACTACGACA TCATCCAGAA TCCCATCGAC 
ATGAGCACCA TCGATCACAA TATACGCACC GATCGCTATG CCACTGTGGA GGATGTCGTC 
TCCGACTACC GGCTGATGTT CTCCAACTGT CGGCAGTACA ACGAGGAGGG CTCTACCATC 
TACGAAGATG CCAACATTCT GGAGCGAGCC CTCAACGAGA AGCTCAAGGA GTTCCCGGGC 
CTCGTGGAGG TGAAAAAACC CCTTCAGAAG TACTCCAAGG TGGGGCGCAA GCTGAAGACG 
GCTCTGGTTA CCGACCGCCT CTGGCAGTTC TATGAGACAC TGCGCGAGTA TCAGGAGCCC 
AAGGGCAAGC GTCAGCTCTC GCTGATTTTC ACCAAGCTGC CGTCGAAGGG CGAGTATCCG 
GACTACTACG ACATCATCAA GGAGCCCATG GACATGGACC GCATTGCCCA GAAGCTGAAG 
CAGTCCGCCT ACGAGACCCT GGACGAGCTG GCCGCCGATT TCCTGCTGAT GCTGGAGAAC 
GCCTGCAAGT ACAACGAGCC CGACTCGCAG ATCTACAAGG ATGCTCTGGT GCTGCAGCAG 
CTGACGCTGC AGTTGAAGCA GCAGCTGCGT ATCGAGCGCG ACTCGCTGCC GGATGTCCCG 
CTGGCCGTGC AGGAGCTGTT CCTCACGCTG TTCACGACCC TGTACAATCA CCAGGATGAG 
GAGGGACGCT GCTACTCCGA CTCCTTGGCC GAGCTGGCCG AGTACGATGA GCTGGGCGAG 
GGGGCCAAGG TGCGCGGCAT TTCGCTGGAC TTGATCAAGC GAAGGCTGGA CAAGGGCGCC 
TACAAGCGAC TGGATATCTA CCAGGAGGAC ATCTTCTCCT GCCTGGAGCG TGCCCGCAAA 
CTGTCGCGCA CGGACTCGGA TATATTTCAG GACTCCATCG AGCTGCAGAC CTACTTCATA 
CGCAAGCGGG ATGAGCTGTG CAAGGACACG CTCAGCTCGC CGGCTCTGAG CTTTACGCTG 
GAGCGCCTGT TGGCCGATGT GGAGGTGATG CGACAGCAGA AGCTGCTGCA AGAGGAGCAG 
GAACAGGAGC AGGACAAAGA TGAGTTCAAT GCCGCCAGCA AGGGTGAGAG CATGACAATC 
AATCAGCAGG TCTACTCCCC CGGCGACTAT GTGTACGTGC AGATGCCGGA GAACAAGATA 
CCCTCGATCG CCTGCATCGA GAGGCTGTGG ACATCGCCCA GCAACGAGAA GCTGATGCAG 
GGCTCGATCT TCGTGCGGCC CCACGAGACG TATCACGTGA CGACGCGGAA GTTCCTCGAG 
AAGGAGGTGT TCAAGAGCAG CATCTCGCAG ACCATCTCCA TGGACAAGGT ACTGGGCATG 
TGCTATGTGA TGCACATCAA GGACTACATC AAGATGCGTC CCGACCGGCT GCAAGAGAAG 
GATGTGTTCG TGTGCGAGTC GCGCTACAAT CTGCAGGGAC GGTGCTTCAA GAAGCTCAAG 
AACTGGCCCC AGATACGCGA GGGCAGCTCC GTGAGGTTTG TGCCGCGCGA CCAGCCGCTG 
GAGCTGAAGC GCGTGATGTC CGTGTTCAAG GAGCGCATCG AGAAGCACAA GGGGGAGCTG 
GAGGAGCTCA AAATGCAGGA GACGATGGTG GAGAAGGAGA AGCCCAATGT GTCCTGCGAC 
CCGCCACCGA ATGCCGAGAA CAACAGTGTC TACTACCAGC AGTACAACAC CATCTGCAGC 
GGGGCCATCA AGACGGGCGA CTTCGTCTAC GTGGCCACCC AAACGGGCAA GCAGTCCGTG 
GCGCAGGTCC AGCAGATCTG GGAGCAGAAC GGTAAGTCAT ACTTCAAGGG TCCCTGGCTG 
CTGCCCCCCA GCGAAACGAC CCCCGCCCTG GGCAAGCAGT TCTTCCGCCA GGAGCTGCTC 
CTCACCACCG TCGAAGAGGT CAGTCCCGTG GTGGGTGTTG TGGGTCGCTG CGCCGTGCTG 
GAGTACGCGG AGTTCATCAA CTCCCGACCC ACAGAGATAG CCGAGAGCGA CGTCTACATC 
TGCGAGTCCG TCTACGACGA GCTGAAGAAG GCCGTGCGCA AACTGGTGGC CGGTAACATG 
CGCAAGTTCC AGCACAGCTC GGCCGTCACC GAGGATGAGA TCTTCTACTT CAAGACTCCC 
ATCAAGCCGG CCAAGGATGT GAAGAGCGAG ATCAACGATC TGAGCATGTT GGAGGACTCG 
ATGGATGGTG ATACACCGTC TCTGAGCTCG GACATAGCGG CCATGTCCTC GCCGGCGCCC 
TCTGTGAACT CCACGCCTCT GACCTCCAAA GTGAAGGCTG CCAAATCGGC CAAGAAATGC 
CTCACCGGCT ACATTCTCTA CTCCAGCGAA GTGCGAAAGG GCATTTGCCA AAGCAATCCA 
GAGGCCTCCT TTGGGGACAT ATCCCGAATG GTGGGCAACG AATGGAAGAA TCTACCGTCC 
AGCGTAAAGC AAAGTTGGGA GGATCGCGCC AGCAAACAGA ACGAGGAGAC GGCAGCCCTG 
CGCCGTGAGA TAGATGACAC GCAGAACAGT GCCAGTCCTT CGTCCCAGGT GAGTGCCACC 
CAGGAGTCCA CAGGTCCACC GCTCACCTTC GAGTGCCTGT GGGACAAGTG CGATTTCCAG 
TTCGAGGAAA TGCCGGACTG CACCGAACAC TGCCTCTCCG AAGCCACCGG CCACATACAG 
CGGCATCCGC CGTCGGGCGT GGAGATTGAG TACGTCTGCC TCTGGCGCAA CTGTTCTCGC 
ATCAAGAAGT CCACCCAGGG ATTCCCCAAC GTGGTGCGGC TCATCAAGCA CGTGCGCGAA 
GTGCATCTGA GCAAGTGCGG AAAGGTTTTG GGCATCACCG AGCGCAGCAA GAACTTTGTG 
CCGCGCCGGC AGAAGCACAT GCCCCAGATG GCTGCCACTG TGCCGCTGCC GCCCAACAAT 
GCCCATTCAC CGCGTGCCGC CAACACCGCT GAGGCAGCGC AGCTGCAACA GCAGGCGCCG 
CACACTCAGC AGATGATGCA GCAGCCGCAG CAAACGATTG TGCTCGGACC ACCGCCAGAG 
CCGATGTTTG TCACCGTGCC GCCGCGCACC ACTCGAGTCA TCCATTCGGA GGCCTACATC 
AAGTACATCG AGAGCCTCCA GACGGGCAGC CACCTCAACG TGGCCGCCTG CAACAACAAC 
TGGCGGCGAG CCCTCACACA CGTGACTCCC GCCCAAGTCG TGCCGAAGGT GCCACTGCCC 
GAACACTGGC TGGGACCCAA TGCCCGCGAT CAGGACAGCG TTGTGCAGGC CCTGTGCCAC 
CTACGCAACT TTATGCTAGA CGATGTGCTG CAGATTCGCC GCAGCTGCAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA10956 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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