LOC6552588 cDNA ORF clone, Drosophila erecta

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC6552588
Entrez Gene ID	6552588
Full Name	GG17413 gene product from transcript GG17413-RB
Synonyms	GG17413,dere_GLEANR_2300
Gene Type	protein-coding
Organism	Drosophila erecta

mRNA and Protein(s)

mRNA	Protein	Name
XM_015154543.1	XP_015010029.1	uncharacterized protein, isoform E
XM_015154545.1	XP_015010031.1	uncharacterized protein, isoform H
XM_001980861.2	XP_001980897.2	uncharacterized protein, isoform F
XM_026981909.1	XP_026837710.1	tight junction protein ZO-1 isoform X3
XM_026981949.1	XP_026837750.1	tight junction protein ZO-1 isoform X11
XM_026981952.1	XP_026837753.1	tight junction protein ZO-1 isoform X12
XM_015154544.1	XP_015010030.1	uncharacterized protein, isoform G
XM_015154548.2	XP_015010034.1	tight junction protein ZO-1 isoform X8
XM_026981923.1	XP_026837724.1	tight junction protein ZO-1 isoform X5
XM_015154540.2	XP_015010026.1	tight junction protein ZO-1 isoform X2
XM_015154546.2	XP_015010032.1	tight junction protein ZO-1 isoform X13
XM_015154540.1	XP_015010026.1	uncharacterized protein, isoform B
XM_015154542.1	XP_015010028.1	uncharacterized protein, isoform D
XM_015154547.1	XP_015010033.1	uncharacterized protein, isoform J
XM_026981913.1	XP_026837714.1	tight junction protein ZO-1 isoform X4
XM_026981926.1	XP_026837727.1	tight junction protein ZO-1 isoform X6
XM_026981939.1	XP_026837740.1	tight junction protein ZO-1 isoform X9
XM_026981907.1	XP_026837708.1	tight junction protein ZO-1 isoform X1
XM_026981920.1	XP_026837721.1	tight junction protein ZO-1 isoform X5
XM_026981945.1	XP_026837746.1	tight junction protein ZO-1 isoform X10
XM_015154541.1	XP_015010027.1	uncharacterized protein, isoform C
XM_015154548.1	XP_015010034.1	uncharacterized protein, isoform K
XM_015154546.1	XP_015010032.1	uncharacterized protein, isoform I
XM_026981931.1	XP_026837732.1	tight junction protein ZO-1 isoform X7

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC6552588 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC6552588 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODi216960	XM_026981952.1 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODi33933	XM_015154546.2 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODi216956	XM_026981931.1 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi216957	XM_026981939.1 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODi216958	XM_026981945.1 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODi216959	XM_026981949.1 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODi33934	XM_015154547.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant J (Dere\GG17413), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODi33935	XM_015154548.2 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi216952	XM_026981907.1 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODi216953	XM_026981909.1 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODi216954	XM_026981913.1 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODi216955	XM_026981920.1 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODi216955	XM_026981923.1 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODi33444	XM_001980861.2 Latest version!	Drosophila erecta uncharacterized protein, transcript variant F (Dere\GG17413), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODi33444	XM_026981926.1 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODi33927	XM_015154540.2 Latest version!	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODi33928	XM_015154541.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant C (Dere\GG17413), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODi33929	XM_015154542.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant D (Dere\GG17413), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODi33930	XM_015154543.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant E (Dere\GG17413), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODi33931	XM_015154544.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant G (Dere\GG17413), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODi33932	XM_015154545.1 Latest version!	Drosophila erecta uncharacterized protein, transcript variant H (Dere\GG17413), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ODi33927	XM_015154540.1	Drosophila erecta uncharacterized protein, transcript variant B (Dere\GG17413), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODi33935	XM_015154548.1	Drosophila erecta uncharacterized protein, transcript variant K (Dere\GG17413), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODi33933	XM_015154546.1	Drosophila erecta uncharacterized protein, transcript variant I (Dere\GG17413), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC6552588 ( XM_026981952.1 ) cDNA ORF clone, Drosophila erecta -> XP_026837753.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ODi216960
Clone ID Related Accession (Same CDS sequence)	XM_026981952.1
Accession Version	XM_026981952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCAGT TCGACTATAC CTGCTATACA GTGCCCGAGG GTTCCACGGA TGACGACTGC 
GATGAGCTGG GCAGCAATGC CACCCTGGAG AACAAGGATC ACTCGGATTG CACCCTGGAA 
TCGGATGCTA CGACCACCAC ATCTTCACTG GAGCGTCATA GATATCGGAA TCGGTATCGC 
TCCACCAGGA GTTTCGGTCC CCAGCGGGTT TCCATGCCCG TTCTCGGATC CAGCCGGTCC 
AGGGAGATGA CGCCCCAGTT GGGAGTGTAC CATGAGGGAC CGGATCGAGA TCCCCAATGG 
GGTTACTTTG TACCCCTGTC GCCCAACTGC TTCTTTAGTC CACCGATGCA GAGGAGGCGT 
TATAGGGAAA AGGAAAACGA TCGCAATCGT CGGGCTGTTA GTTATGGCGG TGACGTCACC 
TCCTCCCCCC ACTCGGCGAC CATACCCCGC GCCGCCCCCC GTCGTGTGGC CTTGATGAAG 
GATCCACCGC CACCGCCGCC ACCCAAGCCG CAATTGGGCT CCAAAGCGGA TTCCGTAGCG 
CATTTGGCCA GCAAATATCC CCAATCCGAG TACAATCTCA TACAGAAAAT CGACTCGAAC 
AGCACACTCA CTGCTCCGGC GCAATATCAA CCGCAGAATT TCTACGCGAA TACCGGAACC 
ATATCCTCCA CGAATGGCTA TGGATCGCTG TTGTGTCACG CCAGTTCGAC GACCAGTCCG 
CTGGCGGTTC GGAAGCGGGA CAAGCTGCTC CATCGGTTCA GCGATGCCGC CACCTTGGGC 
CGGAAGCTCA AGAAGAAGAA GAACACGAAT CGCACCTGCA GATCCATGAC GGAGGCTATT 
GAGATGCTGG CCGATCCCGT CATCGAGGAT GAATTCTTTG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCTCGACGC ACCGACGCTC GAAGAGTCTG 
TCCCGCGAGA ACTGGGACGA CGTGGTCTTC TCCGACAGCA TTTCCAAATT CCCCGCCTAC 
GAGCGAGTCG TTCTCCGCCA TCCTGGCTTT GTTCGACCCG TAGTGCTCTT CGGACCCGTA 
TCCGATCTGG CCCGCGAGCG CTTGGCCAAG GATTTCCCCG ACAAGTTTTC GACTCCACTG 
CAGGATGATG ACAAGTCAGC TGCAACCAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAC 
ATACGCGATG TGATGGATCG TGGCAAGCAC GCCCTGCTGG ACATAACGCC CAATGCAGTG 
GATCGTCTCA ACTACGCCCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG 
CACGTGATCA AGCAGCTGCG CCACGGACTA CCCAAGGCGG CCCACAAGAG CTCCAAGAAG 
CTGCTGGAGC AGACCCAGAA ACTGGAGCGC GTCTGGTCCC ACATCTTTAG CACCCAGATC 
GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ACTCGATCGA TCTGCAGCAG 
AGCGGCGCCG TGTGGATGTC CGAGTCCAAG TTCCCCGATT CCCCCACCGA AATGTTATTC 
CCCCCCTACA TCGCCCAGCC ATGCAAGCTA GCTTGTTGCC GCCCCTTATC ATCTACCTCA 
GTGCCACGCT ACTCGCAGGA TCTGAATCTG AACCAGAATC CGAGTAAATC CTATTCGCTT 
ATCCAAAACC AGGCGCACAC CCCTCCGCCG CCACGCCCTC CGCCCGTGGG TTACGCCCAC 
AGCCAGAGTT TGGCTGAAAA GCCCCGAGAT CGGAGGTCCT ATACGATGGT GCCGATCATA 
AAGCCCGCTT CTTCGATGCC CAGTGGCCTG GCACCACCCG TTGAGCCTGC TCCCATGATG 
CCACTGGATG ATGGTGTCCT GGCCATGGAT GTGTCCAACT CCCAGTATCA CAGTTCTCAG 
CCAAATCTAA TGAGTCAGAG TTATTTGAAC TCGAACTTAA ATGGCGGCAG TACGAGTGCC 
GTAAATCAAT CGAACCAATC CAATGCCACG AGGGGCTTTC AACCCGGACC ATCGACAGTC 
AGCCGCTATG GATTGCGGTC CAATGCCCAG GTGGAACGTA GGACCACGCC CTACTACTAC 
CACGAGCTGC TGCTGCAGAA GAATCTCTTC GACTCCAGCC TGAATCTTCT GCCCCAGCAG 
AGCAAGAACA GCAATCTGCA GCTGGAGCAG CAGCAGCAGC AACAGCATCT TGCAGATGCA 
TCGAATGCAT CCAAAAGGGC ACCGATGGCC AAGCCACAGC AGCGATTGAT TAGCAGTGGT 
CAGGGCAGAA GCACTTACTC CTCCTCGGGA TCACTGAGCA ATCTTCTGGG CAATGGCAAT 
GGCAATGGCA ATGGGTTGAG CAATGGGCAC GAGAGCAGCG ATGGCACTTT GGCGGATGAG 
AGCAGCTTCA CACTGGCCTT TAATAATCTC ACCAATCAAA TAGCCAGTCT CAACAATTCA 
AGTAGCCTAA ACAACAATAA TTGTAGCAAC AGCAGGAGCA TCAACAACAA CAGCAACAGC 
CATACTATCA ATACCAACAA TAATGTTAAT AATGCTGATT CCAACAACAA TGTTGGCCAA 
AGTAACGAAT GCATTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026837753.1
CDS	436..4716
Translation	MSQFDYTCYTVPEGSTDDDCDELGSNATLENKDHSDCTLESDATTTTSSLERHRYRNRYRSTRSFGPQRVSMPVLGSSRSREMTPQLGVYHEGPDRDPQWGYFVPLSPNCFFSPPMQRRRYREKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKFPDSPTEMLFPPYIAQPCKLACCRPLSSTSVPRYSQDLNLNQNPSKSYSLIQNQAHTPPPPRPPPVGYAHSQSLAEKPRDRRSYTMVPIIKPASSMPSGLAPPVEPAPMMPLDDGVLAMDVSNSQYHSSQPNLMSQSYLNSNLNGGSTSAVNQSNQSNATRGFQPGPSTVSRYGLRSNAQVERRTTPYYYHELLLQKNLFDSSLNLLPQQSKNSNLQLEQQQQQQHLADASNASKRAPMAKPQQRLISSGQGRSTYSSSGSLSNLLGNGNGNGNGLSNGHESSDGTLADESSFTLAFNNLTNQIASLNNSSSLNNNNCSNSRSINNNSNSHTINTNNNVNNADSNNNVGQSNECIA

Target ORF information:

RefSeq Version	XM_026981952.1
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026981952.1

ORF Insert Sequence:

ATGTCGCAGT TCGACTATAC CTGCTATACA GTGCCCGAGG GTTCCACGGA TGACGACTGC 
GATGAGCTGG GCAGCAATGC CACCCTGGAG AACAAGGATC ACTCGGATTG CACCCTGGAA 
TCGGATGCTA CGACCACCAC ATCTTCACTG GAGCGTCATA GATATCGGAA TCGGTATCGC 
TCCACCAGGA GTTTCGGTCC CCAGCGGGTT TCCATGCCCG TTCTCGGATC CAGCCGGTCC 
AGGGAGATGA CGCCCCAGTT GGGAGTGTAC CATGAGGGAC CGGATCGAGA TCCCCAATGG 
GGTTACTTTG TACCCCTGTC GCCCAACTGC TTCTTTAGTC CACCGATGCA GAGGAGGCGT 
TATAGGGAAA AGGAAAACGA TCGCAATCGT CGGGCTGTTA GTTATGGCGG TGACGTCACC 
TCCTCCCCCC ACTCGGCGAC CATACCCCGC GCCGCCCCCC GTCGTGTGGC CTTGATGAAG 
GATCCACCGC CACCGCCGCC ACCCAAGCCG CAATTGGGCT CCAAAGCGGA TTCCGTAGCG 
CATTTGGCCA GCAAATATCC CCAATCCGAG TACAATCTCA TACAGAAAAT CGACTCGAAC 
AGCACACTCA CTGCTCCGGC GCAATATCAA CCGCAGAATT TCTACGCGAA TACCGGAACC 
ATATCCTCCA CGAATGGCTA TGGATCGCTG TTGTGTCACG CCAGTTCGAC GACCAGTCCG 
CTGGCGGTTC GGAAGCGGGA CAAGCTGCTC CATCGGTTCA GCGATGCCGC CACCTTGGGC 
CGGAAGCTCA AGAAGAAGAA GAACACGAAT CGCACCTGCA GATCCATGAC GGAGGCTATT 
GAGATGCTGG CCGATCCCGT CATCGAGGAT GAATTCTTTG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCTCGACGC ACCGACGCTC GAAGAGTCTG 
TCCCGCGAGA ACTGGGACGA CGTGGTCTTC TCCGACAGCA TTTCCAAATT CCCCGCCTAC 
GAGCGAGTCG TTCTCCGCCA TCCTGGCTTT GTTCGACCCG TAGTGCTCTT CGGACCCGTA 
TCCGATCTGG CCCGCGAGCG CTTGGCCAAG GATTTCCCCG ACAAGTTTTC GACTCCACTG 
CAGGATGATG ACAAGTCAGC TGCAACCAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAC 
ATACGCGATG TGATGGATCG TGGCAAGCAC GCCCTGCTGG ACATAACGCC CAATGCAGTG 
GATCGTCTCA ACTACGCCCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG 
CACGTGATCA AGCAGCTGCG CCACGGACTA CCCAAGGCGG CCCACAAGAG CTCCAAGAAG 
CTGCTGGAGC AGACCCAGAA ACTGGAGCGC GTCTGGTCCC ACATCTTTAG CACCCAGATC 
GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ACTCGATCGA TCTGCAGCAG 
AGCGGCGCCG TGTGGATGTC CGAGTCCAAG TTCCCCGATT CCCCCACCGA AATGTTATTC 
CCCCCCTACA TCGCCCAGCC ATGCAAGCTA GCTTGTTGCC GCCCCTTATC ATCTACCTCA 
GTGCCACGCT ACTCGCAGGA TCTGAATCTG AACCAGAATC CGAGTAAATC CTATTCGCTT 
ATCCAAAACC AGGCGCACAC CCCTCCGCCG CCACGCCCTC CGCCCGTGGG TTACGCCCAC 
AGCCAGAGTT TGGCTGAAAA GCCCCGAGAT CGGAGGTCCT ATACGATGGT GCCGATCATA 
AAGCCCGCTT CTTCGATGCC CAGTGGCCTG GCACCACCCG TTGAGCCTGC TCCCATGATG 
CCACTGGATG ATGGTGTCCT GGCCATGGAT GTGTCCAACT CCCAGTATCA CAGTTCTCAG 
CCAAATCTAA TGAGTCAGAG TTATTTGAAC TCGAACTTAA ATGGCGGCAG TACGAGTGCC 
GTAAATCAAT CGAACCAATC CAATGCCACG AGGGGCTTTC AACCCGGACC ATCGACAGTC 
AGCCGCTATG GATTGCGGTC CAATGCCCAG GTGGAACGTA GGACCACGCC CTACTACTAC 
CACGAGCTGC TGCTGCAGAA GAATCTCTTC GACTCCAGCC TGAATCTTCT GCCCCAGCAG 
AGCAAGAACA GCAATCTGCA GCTGGAGCAG CAGCAGCAGC AACAGCATCT TGCAGATGCA 
TCGAATGCAT CCAAAAGGGC ACCGATGGCC AAGCCACAGC AGCGATTGAT TAGCAGTGGT 
CAGGGCAGAA GCACTTACTC CTCCTCGGGA TCACTGAGCA ATCTTCTGGG CAATGGCAAT 
GGCAATGGCA ATGGGTTGAG CAATGGGCAC GAGAGCAGCG ATGGCACTTT GGCGGATGAG 
AGCAGCTTCA CACTGGCCTT TAATAATCTC ACCAATCAAA TAGCCAGTCT CAACAATTCA 
AGTAGCCTAA ACAACAATAA TTGTAGCAAC AGCAGGAGCA TCAACAACAA CAGCAACAGC 
CATACTATCA ATACCAACAA TAATGTTAAT AATGCTGATT CCAACAACAA TGTTGGCCAA 
AGTAACGAAT GCATTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154546.2 ) cDNA ORF clone, Drosophila erecta -> XP_015010032.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X14, mRNA.

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CloneID	ODi33933
Clone ID Related Accession (Same CDS sequence)	XM_015154546.2 , XM_015154546.1
Accession Version	XM_015154546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015154546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATAG TCAGCGGATC GGCATCGGGC AACCAGCTCG TCCTGCCAAA CAATCATTTT 
CGCCATGAAT CATTTCAGCA ACAGGGTGAT CGAACCACTT GGGAGTACCA CACGGTGGCA 
GTGACTCGGG TGCCGGGCTA TGGATTCGGC ATCGCCGTTT CCGGTGGACG TGATAATCCG 
CACTTCGCCA ACGGCGATCC CTCGATAGCA GTTAGCGATG TGCTGAAAGG TGGCCCCGCC 
GAGGATCGAT TGCAAGTCAA CGATCGGATA ATCTCGGTGA ACGGCGTCTC CCTGGAGAAC 
GTGGAATATG CCACCGCGGT GCAGGTGTTG CGCGATAGTG GCAACACGGT CCAATTGGTG 
GTCAAGCGTC GCGTGCCCCT CAATCCCATC AATAATGGAG GTGCAGTGCA GCATCAGCAC 
TCGCACAGTC TCAGCTCGGT TGGTCTATTG GCCAATGGAA GTGGAGGAGT TGCACCAACG 
CCCTTGACGA GCCTGAGTCA ACCCAACTCC CTGAACTCTT CGCTGGTGCA GAATGCCAGC 
AGTGGTCAGC CCATCAAGGT GACCCTCACG AAAGGCGGCA AGAAAGATGA CTATGGCGTG 
GTCCTTGGCT GCCGGCTGTT TGTTAAGGAG ATCTCCTCCA AAGCTCGCGA GCAACTTAAT 
GCCAATGGCT ACAGTTTGCA AGAGGGTGAC ATTATCACCC GCATCCACAA CACCAATTGC 
GGGGATACGA TGAGTCTCAA GGAGGCGAAA AAGATCATCG ATGGTTGCAA GGAGCGCTTG 
AACCTAGTGG TTCTACGGGA CATCACCAAC CAAGCAGCTG TTAGCCAACT GAATCTGAAC 
AATTCAGCGA GCCACCAGGC TTCGGCAAAT ATATACGGCA CTCATCAGCC TCAGGTATCC 
GGATGTAGCA GCAGTAACAA CAATCTTGAA GATCCATATC TGCCGGGCGG AGCCAGTTAC 
TCCTCGCAGA ATCTGTACGT GCAACCACCC ACTCGCACCT CCAATGGTCC CAATCTCAAT 
GGCAATGGGC TGAACGACGA AAAGAGTAAT CTCACACCGC GAGGTCGCTC CCGGGGGCCC 
ATTATGGATG GGGTATCTCT GCAGCAGCTG GACAGGCCCG TAACACCGAC GCGTGGCAGG 
AGTGCGGCCA TCGACGAGCC GCCACGTCCA CCGCCGCCAC GAGGATCGAG CGGGGGAGCG 
GCCCAGGAGG ATTTCTACAG CTCTCGCAGG CAATTGTACG AGGAGCGCCA GAGTGCCGAG 
CCGAGATTCA TTTCCTTCCA GAAGGAGGGA AGCGTGGGCA TCCGCCTAAC CGGCGGCAAT 
GAAGCCGGTA TTTTTGTGAC TGCCGTACAA CCGGGATCTC CAGCCTCGCT GCAGGGTCTA 
ATGCCAGGCG ATAAGATCCT GAAGGTCAAC GACATGGACA TGAACGGAGT GACGCGCGAG 
GAGGCTGTTC TCTTTCTGTT GAGCCTGCAG GATCGCATCG ATCTGATTGT GCAGTACTGC 
AAGGAGGAGT ACGACGAGGT GGTGACCAAC CAACGTGGCG ACTCCTTCCA CATCAAGACA 
CATTTCCACT GCGATAATCC CTCCAAAGGC GAGATGGCCT TCAAGGCGGG CGATGTGTTC 
CGGGTGATCG ATACCCTGCA CAATGGTGTA GTCGGTTCCT GGCAGGTGCT GAAGATCGGA 
CGTGGTCACC AGGAGATGCA GCGCGGCGTT ATACCGAACA AGTCGCGGGC CGAAGAACTG 
GCCACTGCCC AGTTCAATGC CACCAAGAAG GAGATGAACG CCAACGAGTC GAGGGGCAAC 
TTCTTCCGGC GACGACGCTC GACGCACCGA CGCTCGAAGA GTCTGTCCCG CGAGAACTGG 
GACGACGTGG TCTTCTCCGA CAGCATTTCC AAATTCCCCG CCTACGAGCG AGTCGTTCTC 
CGCCATCCTG GCTTTGTTCG ACCCGTAGTG CTCTTCGGAC CCGTATCCGA TCTGGCCCGC 
GAGCGCTTGG CCAAGGATTT CCCCGACAAG TTTTCGACTC CACTGCAGGA TGATGACAAG 
TCAGCTGCAA CCAGTGGAAA GTGCCGCATC GTAAGGCTCT CGAACATACG CGATGTGATG 
GATCGTGGCA AGCACGCCCT GCTGGACATA ACGCCCAATG CAGTGGATCG TCTCAACTAC 
GCCCAGTTCT ATCCGGTGGT GATCTTCCTG AAGACAGACA GCAAGCACGT GATCAAGCAG 
CTGCGCCACG GACTACCCAA GGCGGCCCAC AAGAGCTCCA AGAAGCTGCT GGAGCAGACC 
CAGAAACTGG AGCGCGTCTG GTCCCACATC TTTAGCACCC AGATCGCGTT GAGCGACGAG 
GAGTCCTGGT ACCGAAAGTT GCGCGACTCG ATCGATCTGC AGCAGAGCGG CGCCGTGTGG 
ATGTCCGAGT CCAAGCCAGT AGAATCCCTC TCCGACGATT TCCTATTCCC CATGACCACA 
TCCCGACTCT CGTATGCATC AAGTCCCGAA TCCGATTTGG AACTGAGTCC CGGCCCATCC 
GCATCATTGT CGCTTGGCAA TTTGCCGCAG TTGGTTAAAG CGAGCTCAGA TCCATCGATT 
GCCACTAATC AGGATAACTT GGATAGGGAT AGAGACATAA TTGGTGAAGG ACTACCACCT 
CCATATACGG TACCCTACGA CCATGGTGTC CCCGCGAATC CCAATCGCCG CCAGACCATG 
GACTCCAGCA AGTACAGCAT CTACGGCACC AACGTGCCGC CGCAGCCGCA GCAGCCCGGC 
GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA CAGTCTCTGT ACGGGATTAA TGCACCCGAT 
CTGCCGCCCC GCATCGATCG TCAGTCCAAG CCGGGTGATA TGCCACTGAA CACCTCGGGC 
TCGTCGTCGC GAAATGGCAC CCTGGGTCGC AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT 
GTGGTGCAGG ACGATGTCCA GGCGGAATAC ATAACCCGAA ATGCTCTGGT CGGCTCCGGG 
GCGGCTGAAA CCCTGGATCG CCAGCAGCAG CAGCAAACCC ACGCGTCCTT GGAGCGGCAG 
GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC GGAGGAGGCG CCGGAGGAGG TGCATCCACC 
TACGACAGTG TGTCCAGCTA CGACTCGTAC AACAACACAC AAATGGCCAT GCAAAACCTG 
GGCCGACTGG GTCCCAATGC ACCCGACGAT CTCAAATCAG TGCCAAATGC AAGTGGTCGC 
CCCTTGCCTC CGACGGGTCA GTCGCTCGAC TACGGACGCA CTCCGCACGA TCACCGCAGC 
TTCGGAGGAG CCAACGATCT GAACCGACAG AGCAGTCCGG GCAGACCGCA CTACCATGAC 
ATGAATGCGT CCCGTAATAT TGATCCACGC AATGGCACTC CACAGCGTCC TTCGAATCTG 
GGCCTAGAAA GCAGTCCACG CAAGCCTTTG GTGGAGACGA AGACGGATTA CGGCAAATAC 
AGTCGAAACA ACTCCGTATC GCAAGCGGAT TACACCAAGC TACCGAAGAC GGCGCCACAT 
GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC GGCCAAAGCC AGATGAATGG CAGTGGAACG 
CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG CCGGTGCCGC CACCCAAACC CAAGAACTAT 
AGGCCACCGG TGCAAAGTGG TGGCAGCAGT GGCAGCGGTG GCACTACTCC GTGGGAAAAT 
GGGGACTCTG GCTCGCCACG CTCTCCAAAT GGCTTCTATT ATCCACCAAC GCCTTCGCAT 
CATCACTACG GCCAGCAGGC GACTCCCGGT TCACCCAGCA ACGGCCACAT GCAGCCACCT 
CCGCCGCAGC AGCAGCAGCA GCAGCCCACC TACGGCGGAA GTAATGGCAA CTACGGACAG 
GCGCCACCAC CACAACCCTA TCCGCAGGCC AATGGCTACA ACGGGAATGG CCATCACTAC 
AACGGAGGCA GCGGCACGGG ACCCTACATT GCACCACATC GCGGCATGCC GCCGCCAATA 
GGAAACCTGC CGCCCCACAC GCCCGAACGT CATGCCCTGG ACTTGGCCGG AAGTCGGGAG 
CAGCGTGGCT CGGCCTTCGA GCTGTATCGT AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC 
CATCACAACA TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010032.1
CDS	582..4739
Translation	MDIVSGSASGNQLVLPNNHFRHESFQQQGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMR

Target ORF information:

RefSeq Version	XM_015154546.2
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154546.2

ORF Insert Sequence:

ATGGATATAG TCAGCGGATC GGCATCGGGC AACCAGCTCG TCCTGCCAAA CAATCATTTT 
CGCCATGAAT CATTTCAGCA ACAGGGTGAT CGAACCACTT GGGAGTACCA CACGGTGGCA 
GTGACTCGGG TGCCGGGCTA TGGATTCGGC ATCGCCGTTT CCGGTGGACG TGATAATCCG 
CACTTCGCCA ACGGCGATCC CTCGATAGCA GTTAGCGATG TGCTGAAAGG TGGCCCCGCC 
GAGGATCGAT TGCAAGTCAA CGATCGGATA ATCTCGGTGA ACGGCGTCTC CCTGGAGAAC 
GTGGAATATG CCACCGCGGT GCAGGTGTTG CGCGATAGTG GCAACACGGT CCAATTGGTG 
GTCAAGCGTC GCGTGCCCCT CAATCCCATC AATAATGGAG GTGCAGTGCA GCATCAGCAC 
TCGCACAGTC TCAGCTCGGT TGGTCTATTG GCCAATGGAA GTGGAGGAGT TGCACCAACG 
CCCTTGACGA GCCTGAGTCA ACCCAACTCC CTGAACTCTT CGCTGGTGCA GAATGCCAGC 
AGTGGTCAGC CCATCAAGGT GACCCTCACG AAAGGCGGCA AGAAAGATGA CTATGGCGTG 
GTCCTTGGCT GCCGGCTGTT TGTTAAGGAG ATCTCCTCCA AAGCTCGCGA GCAACTTAAT 
GCCAATGGCT ACAGTTTGCA AGAGGGTGAC ATTATCACCC GCATCCACAA CACCAATTGC 
GGGGATACGA TGAGTCTCAA GGAGGCGAAA AAGATCATCG ATGGTTGCAA GGAGCGCTTG 
AACCTAGTGG TTCTACGGGA CATCACCAAC CAAGCAGCTG TTAGCCAACT GAATCTGAAC 
AATTCAGCGA GCCACCAGGC TTCGGCAAAT ATATACGGCA CTCATCAGCC TCAGGTATCC 
GGATGTAGCA GCAGTAACAA CAATCTTGAA GATCCATATC TGCCGGGCGG AGCCAGTTAC 
TCCTCGCAGA ATCTGTACGT GCAACCACCC ACTCGCACCT CCAATGGTCC CAATCTCAAT 
GGCAATGGGC TGAACGACGA AAAGAGTAAT CTCACACCGC GAGGTCGCTC CCGGGGGCCC 
ATTATGGATG GGGTATCTCT GCAGCAGCTG GACAGGCCCG TAACACCGAC GCGTGGCAGG 
AGTGCGGCCA TCGACGAGCC GCCACGTCCA CCGCCGCCAC GAGGATCGAG CGGGGGAGCG 
GCCCAGGAGG ATTTCTACAG CTCTCGCAGG CAATTGTACG AGGAGCGCCA GAGTGCCGAG 
CCGAGATTCA TTTCCTTCCA GAAGGAGGGA AGCGTGGGCA TCCGCCTAAC CGGCGGCAAT 
GAAGCCGGTA TTTTTGTGAC TGCCGTACAA CCGGGATCTC CAGCCTCGCT GCAGGGTCTA 
ATGCCAGGCG ATAAGATCCT GAAGGTCAAC GACATGGACA TGAACGGAGT GACGCGCGAG 
GAGGCTGTTC TCTTTCTGTT GAGCCTGCAG GATCGCATCG ATCTGATTGT GCAGTACTGC 
AAGGAGGAGT ACGACGAGGT GGTGACCAAC CAACGTGGCG ACTCCTTCCA CATCAAGACA 
CATTTCCACT GCGATAATCC CTCCAAAGGC GAGATGGCCT TCAAGGCGGG CGATGTGTTC 
CGGGTGATCG ATACCCTGCA CAATGGTGTA GTCGGTTCCT GGCAGGTGCT GAAGATCGGA 
CGTGGTCACC AGGAGATGCA GCGCGGCGTT ATACCGAACA AGTCGCGGGC CGAAGAACTG 
GCCACTGCCC AGTTCAATGC CACCAAGAAG GAGATGAACG CCAACGAGTC GAGGGGCAAC 
TTCTTCCGGC GACGACGCTC GACGCACCGA CGCTCGAAGA GTCTGTCCCG CGAGAACTGG 
GACGACGTGG TCTTCTCCGA CAGCATTTCC AAATTCCCCG CCTACGAGCG AGTCGTTCTC 
CGCCATCCTG GCTTTGTTCG ACCCGTAGTG CTCTTCGGAC CCGTATCCGA TCTGGCCCGC 
GAGCGCTTGG CCAAGGATTT CCCCGACAAG TTTTCGACTC CACTGCAGGA TGATGACAAG 
TCAGCTGCAA CCAGTGGAAA GTGCCGCATC GTAAGGCTCT CGAACATACG CGATGTGATG 
GATCGTGGCA AGCACGCCCT GCTGGACATA ACGCCCAATG CAGTGGATCG TCTCAACTAC 
GCCCAGTTCT ATCCGGTGGT GATCTTCCTG AAGACAGACA GCAAGCACGT GATCAAGCAG 
CTGCGCCACG GACTACCCAA GGCGGCCCAC AAGAGCTCCA AGAAGCTGCT GGAGCAGACC 
CAGAAACTGG AGCGCGTCTG GTCCCACATC TTTAGCACCC AGATCGCGTT GAGCGACGAG 
GAGTCCTGGT ACCGAAAGTT GCGCGACTCG ATCGATCTGC AGCAGAGCGG CGCCGTGTGG 
ATGTCCGAGT CCAAGCCAGT AGAATCCCTC TCCGACGATT TCCTATTCCC CATGACCACA 
TCCCGACTCT CGTATGCATC AAGTCCCGAA TCCGATTTGG AACTGAGTCC CGGCCCATCC 
GCATCATTGT CGCTTGGCAA TTTGCCGCAG TTGGTTAAAG CGAGCTCAGA TCCATCGATT 
GCCACTAATC AGGATAACTT GGATAGGGAT AGAGACATAA TTGGTGAAGG ACTACCACCT 
CCATATACGG TACCCTACGA CCATGGTGTC CCCGCGAATC CCAATCGCCG CCAGACCATG 
GACTCCAGCA AGTACAGCAT CTACGGCACC AACGTGCCGC CGCAGCCGCA GCAGCCCGGC 
GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA CAGTCTCTGT ACGGGATTAA TGCACCCGAT 
CTGCCGCCCC GCATCGATCG TCAGTCCAAG CCGGGTGATA TGCCACTGAA CACCTCGGGC 
TCGTCGTCGC GAAATGGCAC CCTGGGTCGC AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT 
GTGGTGCAGG ACGATGTCCA GGCGGAATAC ATAACCCGAA ATGCTCTGGT CGGCTCCGGG 
GCGGCTGAAA CCCTGGATCG CCAGCAGCAG CAGCAAACCC ACGCGTCCTT GGAGCGGCAG 
GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC GGAGGAGGCG CCGGAGGAGG TGCATCCACC 
TACGACAGTG TGTCCAGCTA CGACTCGTAC AACAACACAC AAATGGCCAT GCAAAACCTG 
GGCCGACTGG GTCCCAATGC ACCCGACGAT CTCAAATCAG TGCCAAATGC AAGTGGTCGC 
CCCTTGCCTC CGACGGGTCA GTCGCTCGAC TACGGACGCA CTCCGCACGA TCACCGCAGC 
TTCGGAGGAG CCAACGATCT GAACCGACAG AGCAGTCCGG GCAGACCGCA CTACCATGAC 
ATGAATGCGT CCCGTAATAT TGATCCACGC AATGGCACTC CACAGCGTCC TTCGAATCTG 
GGCCTAGAAA GCAGTCCACG CAAGCCTTTG GTGGAGACGA AGACGGATTA CGGCAAATAC 
AGTCGAAACA ACTCCGTATC GCAAGCGGAT TACACCAAGC TACCGAAGAC GGCGCCACAT 
GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC GGCCAAAGCC AGATGAATGG CAGTGGAACG 
CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG CCGGTGCCGC CACCCAAACC CAAGAACTAT 
AGGCCACCGG TGCAAAGTGG TGGCAGCAGT GGCAGCGGTG GCACTACTCC GTGGGAAAAT 
GGGGACTCTG GCTCGCCACG CTCTCCAAAT GGCTTCTATT ATCCACCAAC GCCTTCGCAT 
CATCACTACG GCCAGCAGGC GACTCCCGGT TCACCCAGCA ACGGCCACAT GCAGCCACCT 
CCGCCGCAGC AGCAGCAGCA GCAGCCCACC TACGGCGGAA GTAATGGCAA CTACGGACAG 
GCGCCACCAC CACAACCCTA TCCGCAGGCC AATGGCTACA ACGGGAATGG CCATCACTAC 
AACGGAGGCA GCGGCACGGG ACCCTACATT GCACCACATC GCGGCATGCC GCCGCCAATA 
GGAAACCTGC CGCCCCACAC GCCCGAACGT CATGCCCTGG ACTTGGCCGG AAGTCGGGAG 
CAGCGTGGCT CGGCCTTCGA GCTGTATCGT AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC 
CATCACAACA TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154546.1 ) cDNA ORF clone, Drosophila erecta -> XP_015010032.1 Drosophila erecta uncharacterized protein, transcript variant I (Dere\GG17413), mRNA.

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CloneID	ODi33933
Clone ID Related Accession (Same CDS sequence)	XM_015154546.2 , XM_015154546.1
Accession Version	XM_015154546.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform I
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956552). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATAG TCAGCGGATC GGCATCGGGC AACCAGCTCG TCCTGCCAAA CAATCATTTT 
CGCCATGAAT CATTTCAGCA ACAGGGTGAT CGAACCACTT GGGAGTACCA CACGGTGGCA 
GTGACTCGGG TGCCGGGCTA TGGATTCGGC ATCGCCGTTT CCGGTGGACG TGATAATCCG 
CACTTCGCCA ACGGCGATCC CTCGATAGCA GTTAGCGATG TGCTGAAAGG TGGCCCCGCC 
GAGGATCGAT TGCAAGTCAA CGATCGGATA ATCTCGGTGA ACGGCGTCTC CCTGGAGAAC 
GTGGAATATG CCACCGCGGT GCAGGTGTTG CGCGATAGTG GCAACACGGT CCAATTGGTG 
GTCAAGCGTC GCGTGCCCCT CAATCCCATC AATAATGGAG GTGCAGTGCA GCATCAGCAC 
TCGCACAGTC TCAGCTCGGT TGGTCTATTG GCCAATGGAA GTGGAGGAGT TGCACCAACG 
CCCTTGACGA GCCTGAGTCA ACCCAACTCC CTGAACTCTT CGCTGGTGCA GAATGCCAGC 
AGTGGTCAGC CCATCAAGGT GACCCTCACG AAAGGCGGCA AGAAAGATGA CTATGGCGTG 
GTCCTTGGCT GCCGGCTGTT TGTTAAGGAG ATCTCCTCCA AAGCTCGCGA GCAACTTAAT 
GCCAATGGCT ACAGTTTGCA AGAGGGTGAC ATTATCACCC GCATCCACAA CACCAATTGC 
GGGGATACGA TGAGTCTCAA GGAGGCGAAA AAGATCATCG ATGGTTGCAA GGAGCGCTTG 
AACCTAGTGG TTCTACGGGA CATCACCAAC CAAGCAGCTG TTAGCCAACT GAATCTGAAC 
AATTCAGCGA GCCACCAGGC TTCGGCAAAT ATATACGGCA CTCATCAGCC TCAGGTATCC 
GGATGTAGCA GCAGTAACAA CAATCTTGAA GATCCATATC TGCCGGGCGG AGCCAGTTAC 
TCCTCGCAGA ATCTGTACGT GCAACCACCC ACTCGCACCT CCAATGGTCC CAATCTCAAT 
GGCAATGGGC TGAACGACGA AAAGAGTAAT CTCACACCGC GAGGTCGCTC CCGGGGGCCC 
ATTATGGATG GGGTATCTCT GCAGCAGCTG GACAGGCCCG TAACACCGAC GCGTGGCAGG 
AGTGCGGCCA TCGACGAGCC GCCACGTCCA CCGCCGCCAC GAGGATCGAG CGGGGGAGCG 
GCCCAGGAGG ATTTCTACAG CTCTCGCAGG CAATTGTACG AGGAGCGCCA GAGTGCCGAG 
CCGAGATTCA TTTCCTTCCA GAAGGAGGGA AGCGTGGGCA TCCGCCTAAC CGGCGGCAAT 
GAAGCCGGTA TTTTTGTGAC TGCCGTACAA CCGGGATCTC CAGCCTCGCT GCAGGGTCTA 
ATGCCAGGCG ATAAGATCCT GAAGGTCAAC GACATGGACA TGAACGGAGT GACGCGCGAG 
GAGGCTGTTC TCTTTCTGTT GAGCCTGCAG GATCGCATCG ATCTGATTGT GCAGTACTGC 
AAGGAGGAGT ACGACGAGGT GGTGACCAAC CAACGTGGCG ACTCCTTCCA CATCAAGACA 
CATTTCCACT GCGATAATCC CTCCAAAGGC GAGATGGCCT TCAAGGCGGG CGATGTGTTC 
CGGGTGATCG ATACCCTGCA CAATGGTGTA GTCGGTTCCT GGCAGGTGCT GAAGATCGGA 
CGTGGTCACC AGGAGATGCA GCGCGGCGTT ATACCGAACA AGTCGCGGGC CGAAGAACTG 
GCCACTGCCC AGTTCAATGC CACCAAGAAG GAGATGAACG CCAACGAGTC GAGGGGCAAC 
TTCTTCCGGC GACGACGCTC GACGCACCGA CGCTCGAAGA GTCTGTCCCG CGAGAACTGG 
GACGACGTGG TCTTCTCCGA CAGCATTTCC AAATTCCCCG CCTACGAGCG AGTCGTTCTC 
CGCCATCCTG GCTTTGTTCG ACCCGTAGTG CTCTTCGGAC CCGTATCCGA TCTGGCCCGC 
GAGCGCTTGG CCAAGGATTT CCCCGACAAG TTTTCGACTC CACTGCAGGA TGATGACAAG 
TCAGCTGCAA CCAGTGGAAA GTGCCGCATC GTAAGGCTCT CGAACATACG CGATGTGATG 
GATCGTGGCA AGCACGCCCT GCTGGACATA ACGCCCAATG CAGTGGATCG TCTCAACTAC 
GCCCAGTTCT ATCCGGTGGT GATCTTCCTG AAGACAGACA GCAAGCACGT GATCAAGCAG 
CTGCGCCACG GACTACCCAA GGCGGCCCAC AAGAGCTCCA AGAAGCTGCT GGAGCAGACC 
CAGAAACTGG AGCGCGTCTG GTCCCACATC TTTAGCACCC AGATCGCGTT GAGCGACGAG 
GAGTCCTGGT ACCGAAAGTT GCGCGACTCG ATCGATCTGC AGCAGAGCGG CGCCGTGTGG 
ATGTCCGAGT CCAAGCCAGT AGAATCCCTC TCCGACGATT TCCTATTCCC CATGACCACA 
TCCCGACTCT CGTATGCATC AAGTCCCGAA TCCGATTTGG AACTGAGTCC CGGCCCATCC 
GCATCATTGT CGCTTGGCAA TTTGCCGCAG TTGGTTAAAG CGAGCTCAGA TCCATCGATT 
GCCACTAATC AGGATAACTT GGATAGGGAT AGAGACATAA TTGGTGAAGG ACTACCACCT 
CCATATACGG TACCCTACGA CCATGGTGTC CCCGCGAATC CCAATCGCCG CCAGACCATG 
GACTCCAGCA AGTACAGCAT CTACGGCACC AACGTGCCGC CGCAGCCGCA GCAGCCCGGC 
GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA CAGTCTCTGT ACGGGATTAA TGCACCCGAT 
CTGCCGCCCC GCATCGATCG TCAGTCCAAG CCGGGTGATA TGCCACTGAA CACCTCGGGC 
TCGTCGTCGC GAAATGGCAC CCTGGGTCGC AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT 
GTGGTGCAGG ACGATGTCCA GGCGGAATAC ATAACCCGAA ATGCTCTGGT CGGCTCCGGG 
GCGGCTGAAA CCCTGGATCG CCAGCAGCAG CAGCAAACCC ACGCGTCCTT GGAGCGGCAG 
GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC GGAGGAGGCG CCGGAGGAGG TGCATCCACC 
TACGACAGTG TGTCCAGCTA CGACTCGTAC AACAACACAC AAATGGCCAT GCAAAACCTG 
GGCCGACTGG GTCCCAATGC ACCCGACGAT CTCAAATCAG TGCCAAATGC AAGTGGTCGC 
CCCTTGCCTC CGACGGGTCA GTCGCTCGAC TACGGACGCA CTCCGCACGA TCACCGCAGC 
TTCGGAGGAG CCAACGATCT GAACCGACAG AGCAGTCCGG GCAGACCGCA CTACCATGAC 
ATGAATGCGT CCCGTAATAT TGATCCACGC AATGGCACTC CACAGCGTCC TTCGAATCTG 
GGCCTAGAAA GCAGTCCACG CAAGCCTTTG GTGGAGACGA AGACGGATTA CGGCAAATAC 
AGTCGAAACA ACTCCGTATC GCAAGCGGAT TACACCAAGC TACCGAAGAC GGCGCCACAT 
GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC GGCCAAAGCC AGATGAATGG CAGTGGAACG 
CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG CCGGTGCCGC CACCCAAACC CAAGAACTAT 
AGGCCACCGG TGCAAAGTGG TGGCAGCAGT GGCAGCGGTG GCACTACTCC GTGGGAAAAT 
GGGGACTCTG GCTCGCCACG CTCTCCAAAT GGCTTCTATT ATCCACCAAC GCCTTCGCAT 
CATCACTACG GCCAGCAGGC GACTCCCGGT TCACCCAGCA ACGGCCACAT GCAGCCACCT 
CCGCCGCAGC AGCAGCAGCA GCAGCCCACC TACGGCGGAA GTAATGGCAA CTACGGACAG 
GCGCCACCAC CACAACCCTA TCCGCAGGCC AATGGCTACA ACGGGAATGG CCATCACTAC 
AACGGAGGCA GCGGCACGGG ACCCTACATT GCACCACATC GCGGCATGCC GCCGCCAATA 
GGAAACCTGC CGCCCCACAC GCCCGAACGT CATGCCCTGG ACTTGGCCGG AAGTCGGGAG 
CAGCGTGGCT CGGCCTTCGA GCTGTATCGT AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC 
CATCACAACA TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010032.1
CDS	546..4703
Translation	MDIVSGSASGNQLVLPNNHFRHESFQQQGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMR

Target ORF information:

RefSeq Version	XM_015154546.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant I (Dere\GG17413), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154546.1

ORF Insert Sequence:

ATGGATATAG TCAGCGGATC GGCATCGGGC AACCAGCTCG TCCTGCCAAA CAATCATTTT 
CGCCATGAAT CATTTCAGCA ACAGGGTGAT CGAACCACTT GGGAGTACCA CACGGTGGCA 
GTGACTCGGG TGCCGGGCTA TGGATTCGGC ATCGCCGTTT CCGGTGGACG TGATAATCCG 
CACTTCGCCA ACGGCGATCC CTCGATAGCA GTTAGCGATG TGCTGAAAGG TGGCCCCGCC 
GAGGATCGAT TGCAAGTCAA CGATCGGATA ATCTCGGTGA ACGGCGTCTC CCTGGAGAAC 
GTGGAATATG CCACCGCGGT GCAGGTGTTG CGCGATAGTG GCAACACGGT CCAATTGGTG 
GTCAAGCGTC GCGTGCCCCT CAATCCCATC AATAATGGAG GTGCAGTGCA GCATCAGCAC 
TCGCACAGTC TCAGCTCGGT TGGTCTATTG GCCAATGGAA GTGGAGGAGT TGCACCAACG 
CCCTTGACGA GCCTGAGTCA ACCCAACTCC CTGAACTCTT CGCTGGTGCA GAATGCCAGC 
AGTGGTCAGC CCATCAAGGT GACCCTCACG AAAGGCGGCA AGAAAGATGA CTATGGCGTG 
GTCCTTGGCT GCCGGCTGTT TGTTAAGGAG ATCTCCTCCA AAGCTCGCGA GCAACTTAAT 
GCCAATGGCT ACAGTTTGCA AGAGGGTGAC ATTATCACCC GCATCCACAA CACCAATTGC 
GGGGATACGA TGAGTCTCAA GGAGGCGAAA AAGATCATCG ATGGTTGCAA GGAGCGCTTG 
AACCTAGTGG TTCTACGGGA CATCACCAAC CAAGCAGCTG TTAGCCAACT GAATCTGAAC 
AATTCAGCGA GCCACCAGGC TTCGGCAAAT ATATACGGCA CTCATCAGCC TCAGGTATCC 
GGATGTAGCA GCAGTAACAA CAATCTTGAA GATCCATATC TGCCGGGCGG AGCCAGTTAC 
TCCTCGCAGA ATCTGTACGT GCAACCACCC ACTCGCACCT CCAATGGTCC CAATCTCAAT 
GGCAATGGGC TGAACGACGA AAAGAGTAAT CTCACACCGC GAGGTCGCTC CCGGGGGCCC 
ATTATGGATG GGGTATCTCT GCAGCAGCTG GACAGGCCCG TAACACCGAC GCGTGGCAGG 
AGTGCGGCCA TCGACGAGCC GCCACGTCCA CCGCCGCCAC GAGGATCGAG CGGGGGAGCG 
GCCCAGGAGG ATTTCTACAG CTCTCGCAGG CAATTGTACG AGGAGCGCCA GAGTGCCGAG 
CCGAGATTCA TTTCCTTCCA GAAGGAGGGA AGCGTGGGCA TCCGCCTAAC CGGCGGCAAT 
GAAGCCGGTA TTTTTGTGAC TGCCGTACAA CCGGGATCTC CAGCCTCGCT GCAGGGTCTA 
ATGCCAGGCG ATAAGATCCT GAAGGTCAAC GACATGGACA TGAACGGAGT GACGCGCGAG 
GAGGCTGTTC TCTTTCTGTT GAGCCTGCAG GATCGCATCG ATCTGATTGT GCAGTACTGC 
AAGGAGGAGT ACGACGAGGT GGTGACCAAC CAACGTGGCG ACTCCTTCCA CATCAAGACA 
CATTTCCACT GCGATAATCC CTCCAAAGGC GAGATGGCCT TCAAGGCGGG CGATGTGTTC 
CGGGTGATCG ATACCCTGCA CAATGGTGTA GTCGGTTCCT GGCAGGTGCT GAAGATCGGA 
CGTGGTCACC AGGAGATGCA GCGCGGCGTT ATACCGAACA AGTCGCGGGC CGAAGAACTG 
GCCACTGCCC AGTTCAATGC CACCAAGAAG GAGATGAACG CCAACGAGTC GAGGGGCAAC 
TTCTTCCGGC GACGACGCTC GACGCACCGA CGCTCGAAGA GTCTGTCCCG CGAGAACTGG 
GACGACGTGG TCTTCTCCGA CAGCATTTCC AAATTCCCCG CCTACGAGCG AGTCGTTCTC 
CGCCATCCTG GCTTTGTTCG ACCCGTAGTG CTCTTCGGAC CCGTATCCGA TCTGGCCCGC 
GAGCGCTTGG CCAAGGATTT CCCCGACAAG TTTTCGACTC CACTGCAGGA TGATGACAAG 
TCAGCTGCAA CCAGTGGAAA GTGCCGCATC GTAAGGCTCT CGAACATACG CGATGTGATG 
GATCGTGGCA AGCACGCCCT GCTGGACATA ACGCCCAATG CAGTGGATCG TCTCAACTAC 
GCCCAGTTCT ATCCGGTGGT GATCTTCCTG AAGACAGACA GCAAGCACGT GATCAAGCAG 
CTGCGCCACG GACTACCCAA GGCGGCCCAC AAGAGCTCCA AGAAGCTGCT GGAGCAGACC 
CAGAAACTGG AGCGCGTCTG GTCCCACATC TTTAGCACCC AGATCGCGTT GAGCGACGAG 
GAGTCCTGGT ACCGAAAGTT GCGCGACTCG ATCGATCTGC AGCAGAGCGG CGCCGTGTGG 
ATGTCCGAGT CCAAGCCAGT AGAATCCCTC TCCGACGATT TCCTATTCCC CATGACCACA 
TCCCGACTCT CGTATGCATC AAGTCCCGAA TCCGATTTGG AACTGAGTCC CGGCCCATCC 
GCATCATTGT CGCTTGGCAA TTTGCCGCAG TTGGTTAAAG CGAGCTCAGA TCCATCGATT 
GCCACTAATC AGGATAACTT GGATAGGGAT AGAGACATAA TTGGTGAAGG ACTACCACCT 
CCATATACGG TACCCTACGA CCATGGTGTC CCCGCGAATC CCAATCGCCG CCAGACCATG 
GACTCCAGCA AGTACAGCAT CTACGGCACC AACGTGCCGC CGCAGCCGCA GCAGCCCGGC 
GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA CAGTCTCTGT ACGGGATTAA TGCACCCGAT 
CTGCCGCCCC GCATCGATCG TCAGTCCAAG CCGGGTGATA TGCCACTGAA CACCTCGGGC 
TCGTCGTCGC GAAATGGCAC CCTGGGTCGC AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT 
GTGGTGCAGG ACGATGTCCA GGCGGAATAC ATAACCCGAA ATGCTCTGGT CGGCTCCGGG 
GCGGCTGAAA CCCTGGATCG CCAGCAGCAG CAGCAAACCC ACGCGTCCTT GGAGCGGCAG 
GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC GGAGGAGGCG CCGGAGGAGG TGCATCCACC 
TACGACAGTG TGTCCAGCTA CGACTCGTAC AACAACACAC AAATGGCCAT GCAAAACCTG 
GGCCGACTGG GTCCCAATGC ACCCGACGAT CTCAAATCAG TGCCAAATGC AAGTGGTCGC 
CCCTTGCCTC CGACGGGTCA GTCGCTCGAC TACGGACGCA CTCCGCACGA TCACCGCAGC 
TTCGGAGGAG CCAACGATCT GAACCGACAG AGCAGTCCGG GCAGACCGCA CTACCATGAC 
ATGAATGCGT CCCGTAATAT TGATCCACGC AATGGCACTC CACAGCGTCC TTCGAATCTG 
GGCCTAGAAA GCAGTCCACG CAAGCCTTTG GTGGAGACGA AGACGGATTA CGGCAAATAC 
AGTCGAAACA ACTCCGTATC GCAAGCGGAT TACACCAAGC TACCGAAGAC GGCGCCACAT 
GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC GGCCAAAGCC AGATGAATGG CAGTGGAACG 
CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG CCGGTGCCGC CACCCAAACC CAAGAACTAT 
AGGCCACCGG TGCAAAGTGG TGGCAGCAGT GGCAGCGGTG GCACTACTCC GTGGGAAAAT 
GGGGACTCTG GCTCGCCACG CTCTCCAAAT GGCTTCTATT ATCCACCAAC GCCTTCGCAT 
CATCACTACG GCCAGCAGGC GACTCCCGGT TCACCCAGCA ACGGCCACAT GCAGCCACCT 
CCGCCGCAGC AGCAGCAGCA GCAGCCCACC TACGGCGGAA GTAATGGCAA CTACGGACAG 
GCGCCACCAC CACAACCCTA TCCGCAGGCC AATGGCTACA ACGGGAATGG CCATCACTAC 
AACGGAGGCA GCGGCACGGG ACCCTACATT GCACCACATC GCGGCATGCC GCCGCCAATA 
GGAAACCTGC CGCCCCACAC GCCCGAACGT CATGCCCTGG ACTTGGCCGG AAGTCGGGAG 
CAGCGTGGCT CGGCCTTCGA GCTGTATCGT AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC 
CATCACAACA TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_026981931.1 ) cDNA ORF clone, Drosophila erecta -> XP_026837732.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X8, mRNA.

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CloneID	ODi216956
Clone ID Related Accession (Same CDS sequence)	XM_026981931.1
Accession Version	XM_026981931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCAGCTC GACGCACCGA 
CGCTCGAAGA GTCTGTCCCG CGAGAACTGG GACGACGTGG TCTTCTCCGA CAGCATTTCC 
AAATTCCCCG CCTACGAGCG AGTCGTTCTC CGCCATCCTG GCTTTGTTCG ACCCGTAGTG 
CTCTTCGGAC CCGTATCCGA TCTGGCCCGC GAGCGCTTGG CCAAGGATTT CCCCGACAAG 
TTTTCGACTC CACTGCAGGA TGATGACAAG TCAGCTGCAA CCAGTGGAAA GTGCCGCATC 
GTAAGGCTCT CGAACATACG CGATGTGATG GATCGTGGCA AGCACGCCCT GCTGGACATA 
ACGCCCAATG CAGTGGATCG TCTCAACTAC GCCCAGTTCT ATCCGGTGGT GATCTTCCTG 
AAGACAGACA GCAAGCACGT GATCAAGCAG CTGCGCCACG GACTACCCAA GGCGGCCCAC 
AAGAGCTCCA AGAAGCTGCT GGAGCAGACC CAGAAACTGG AGCGCGTCTG GTCCCACATC 
TTTAGCACCC AGATCGCGTT GAGCGACGAG GAGTCCTGGT ACCGAAAGTT GCGCGACTCG 
ATCGATCTGC AGCAGAGCGG CGCCGTGTGG ATGTCCGAGT CCAAGCCAGT AGAATCCCTC 
TCCGACGATT TCCTATTCCC CATGACCACA TCCCGACTCT CGTATGCATC AAGTCCCGAA 
TCCGATTTGG AACTGAGTCC CGGCCCATCC GCATCATTGT CGCTTGGCAA TTTGCCGCAG 
TTGGTTAAAG CGAGCTCAGA TCCATCGATT GCCACTAATC AGGATAACTT GGATAGGGAT 
AGAGACATAA TTGGTGAAGG ACTACCACCT CCATATACGG TACCCTACGA CCATGGTGTC 
CCCGCGAATC CCAATCGCCG CCAGACCATG GACTCCAGCA AGTACAGCAT CTACGGCACC 
AACGTGCCGC CGCAGCCGCA GCAGCCCGGC GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA 
CAGTCTCTGT ACGGGATTAA TGCACCCGAT CTGCCGCCCC GCATCGATCG TCAGTCCAAG 
CCGGGTGATA TGCCACTGAA CACCTCGGGC TCGTCGTCGC GAAATGGCAC CCTGGGTCGC 
AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT GTGGTGCAGG ACGATGTCCA GGCGGAATAC 
ATAACCCGAA ATGCTCTGGT CGGCTCCGGG GCGGCTGAAA CCCTGGATCG CCAGCAGCAG 
CAGCAAACCC ACGCGTCCTT GGAGCGGCAG GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC 
GGAGGAGGCG CCGGAGGAGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC 
AACAACACAC AAATGGCCAT GCAAAACCTG GGCCGACTGG GTCCCAATGC ACCCGACGAT 
CTCAAATCAG TGCCAAATGC AAGTGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCTCGAC 
TACGGACGCA CTCCGCACGA TCACCGCAGC TTCGGAGGAG CCAACGATCT GAACCGACAG 
AGCAGTCCGG GCAGACCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT TGATCCACGC 
AATGGCACTC CACAGCGTCC TTCGAATCTG GGCCTAGAAA GCAGTCCACG CAAGCCTTTG 
GTGGAGACGA AGACGGATTA CGGCAAATAC AGTCGAAACA ACTCCGTATC GCAAGCGGAT 
TACACCAAGC TACCGAAGAC GGCGCCACAT GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC 
GGCCAAAGCC AGATGAATGG CAGTGGAACG CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG 
CCGGTGCCGC CACCCAAACC CAAGAACTAT AGGCCACCGG TGCAAAGTGG TGGCAGCAGT 
GGCAGCGGTG GCACTACTCC GTGGGAAAAT GGGGACTCTG GCTCGCCACG CTCTCCAAAT 
GGCTTCTATT ATCCACCAAC GCCTTCGCAT CATCACTACG GCCAGCAGGC GACTCCCGGT 
TCACCCAGCA ACGGCCACAT GCAGCCACCT CCGCCGCAGC AGCAGCAGCA GCAGCCCACC 
TACGGCGGAA GTAATGGCAA CTACGGACAG GCGCCACCAC CACAACCCTA TCCGCAGGCC 
AATGGCTACA ACGGGAATGG CCATCACTAC AACGGAGGCA GCGGCACGGG ACCCTACATT 
GCACCACATC GCGGCATGCC GCCGCCAATA GGAAACCTGC CGCCCCACAC GCCCGAACGT 
CATGCCCTGG ACTTGGCCGG AAGTCGGGAG CAGCGTGGCT CGGCCTTCGA GCTGTATCGT 
AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC CATCACAACA TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026837732.1
CDS	652..5859
Translation	MTREEEEEVRSLLSRHQERIMLDLGSTDLLAVLVKNSVLSQSEEDLLLKPYNAVTPTPSAAASATVEPPGSAPSLTKRKLSAVASSGSTASGGSAGSGGSGSSRCASVNGDANCDSRSLTPSGGGPCSVLNDAEILRVQCSNLIEIIAKNGFEKFKQFCYAIECECPQLIEDLINDRLKTDAANLEDSRDGEKMQKEIETIDYIDKEKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMR

Target ORF information:

RefSeq Version	XM_026981931.1
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026981931.1

ORF Insert Sequence:

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCAGCTC GACGCACCGA 
CGCTCGAAGA GTCTGTCCCG CGAGAACTGG GACGACGTGG TCTTCTCCGA CAGCATTTCC 
AAATTCCCCG CCTACGAGCG AGTCGTTCTC CGCCATCCTG GCTTTGTTCG ACCCGTAGTG 
CTCTTCGGAC CCGTATCCGA TCTGGCCCGC GAGCGCTTGG CCAAGGATTT CCCCGACAAG 
TTTTCGACTC CACTGCAGGA TGATGACAAG TCAGCTGCAA CCAGTGGAAA GTGCCGCATC 
GTAAGGCTCT CGAACATACG CGATGTGATG GATCGTGGCA AGCACGCCCT GCTGGACATA 
ACGCCCAATG CAGTGGATCG TCTCAACTAC GCCCAGTTCT ATCCGGTGGT GATCTTCCTG 
AAGACAGACA GCAAGCACGT GATCAAGCAG CTGCGCCACG GACTACCCAA GGCGGCCCAC 
AAGAGCTCCA AGAAGCTGCT GGAGCAGACC CAGAAACTGG AGCGCGTCTG GTCCCACATC 
TTTAGCACCC AGATCGCGTT GAGCGACGAG GAGTCCTGGT ACCGAAAGTT GCGCGACTCG 
ATCGATCTGC AGCAGAGCGG CGCCGTGTGG ATGTCCGAGT CCAAGCCAGT AGAATCCCTC 
TCCGACGATT TCCTATTCCC CATGACCACA TCCCGACTCT CGTATGCATC AAGTCCCGAA 
TCCGATTTGG AACTGAGTCC CGGCCCATCC GCATCATTGT CGCTTGGCAA TTTGCCGCAG 
TTGGTTAAAG CGAGCTCAGA TCCATCGATT GCCACTAATC AGGATAACTT GGATAGGGAT 
AGAGACATAA TTGGTGAAGG ACTACCACCT CCATATACGG TACCCTACGA CCATGGTGTC 
CCCGCGAATC CCAATCGCCG CCAGACCATG GACTCCAGCA AGTACAGCAT CTACGGCACC 
AACGTGCCGC CGCAGCCGCA GCAGCCCGGC GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA 
CAGTCTCTGT ACGGGATTAA TGCACCCGAT CTGCCGCCCC GCATCGATCG TCAGTCCAAG 
CCGGGTGATA TGCCACTGAA CACCTCGGGC TCGTCGTCGC GAAATGGCAC CCTGGGTCGC 
AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT GTGGTGCAGG ACGATGTCCA GGCGGAATAC 
ATAACCCGAA ATGCTCTGGT CGGCTCCGGG GCGGCTGAAA CCCTGGATCG CCAGCAGCAG 
CAGCAAACCC ACGCGTCCTT GGAGCGGCAG GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC 
GGAGGAGGCG CCGGAGGAGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC 
AACAACACAC AAATGGCCAT GCAAAACCTG GGCCGACTGG GTCCCAATGC ACCCGACGAT 
CTCAAATCAG TGCCAAATGC AAGTGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCTCGAC 
TACGGACGCA CTCCGCACGA TCACCGCAGC TTCGGAGGAG CCAACGATCT GAACCGACAG 
AGCAGTCCGG GCAGACCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT TGATCCACGC 
AATGGCACTC CACAGCGTCC TTCGAATCTG GGCCTAGAAA GCAGTCCACG CAAGCCTTTG 
GTGGAGACGA AGACGGATTA CGGCAAATAC AGTCGAAACA ACTCCGTATC GCAAGCGGAT 
TACACCAAGC TACCGAAGAC GGCGCCACAT GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC 
GGCCAAAGCC AGATGAATGG CAGTGGAACG CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG 
CCGGTGCCGC CACCCAAACC CAAGAACTAT AGGCCACCGG TGCAAAGTGG TGGCAGCAGT 
GGCAGCGGTG GCACTACTCC GTGGGAAAAT GGGGACTCTG GCTCGCCACG CTCTCCAAAT 
GGCTTCTATT ATCCACCAAC GCCTTCGCAT CATCACTACG GCCAGCAGGC GACTCCCGGT 
TCACCCAGCA ACGGCCACAT GCAGCCACCT CCGCCGCAGC AGCAGCAGCA GCAGCCCACC 
TACGGCGGAA GTAATGGCAA CTACGGACAG GCGCCACCAC CACAACCCTA TCCGCAGGCC 
AATGGCTACA ACGGGAATGG CCATCACTAC AACGGAGGCA GCGGCACGGG ACCCTACATT 
GCACCACATC GCGGCATGCC GCCGCCAATA GGAAACCTGC CGCCCCACAC GCCCGAACGT 
CATGCCCTGG ACTTGGCCGG AAGTCGGGAG CAGCGTGGCT CGGCCTTCGA GCTGTATCGT 
AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC CATCACAACA TGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_026981939.1 ) cDNA ORF clone, Drosophila erecta -> XP_026837740.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X10, mRNA.

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CloneID	ODi216957
Clone ID Related Accession (Same CDS sequence)	XM_026981939.1
Accession Version	XM_026981939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCAGCTC GACGCACCGA 
CGCTCGAAGA GTCTGTCCCG CGAGAACTGG GACGACGTGG TCTTCTCCGA CAGCATTTCC 
AAATTCCCCG CCTACGAGCG AGTCGTTCTC CGCCATCCTG GCTTTGTTCG ACCCGTAGTG 
CTCTTCGGAC CCGTATCCGA TCTGGCCCGC GAGCGCTTGG CCAAGGATTT CCCCGACAAG 
TTTTCGACTC CACTGCAGGA TGATGACAAG TCAGCTGCAA CCAGTGGAAA GTGCCGCATC 
GTAAGGCTCT CGAACATACG CGATGTGATG GATCGTGGCA AGCACGCCCT GCTGGACATA 
ACGCCCAATG CAGTGGATCG TCTCAACTAC GCCCAGTTCT ATCCGGTGGT GATCTTCCTG 
AAGACAGACA GCAAGCACGT GATCAAGCAG CTGCGCCACG GACTACCCAA GGCGGCCCAC 
AAGAGCTCCA AGAAGCTGCT GGAGCAGACC CAGAAACTGG AGCGCGTCTG GTCCCACATC 
TTTAGCACCC AGATCGCGTT GAGCGACGAG GAGTCCTGGT ACCGAAAGTT GCGCGACTCG 
ATCGATCTGC AGCAGAGCGG CGCCGTGTGG ATGTCCGAGT CCAAGCCAGT AGAATCCCTC 
TCCGACGATT TCCTATTCCC CATGACCACA TCCCGACTCT CGTATGCATC AAGTCCCGAA 
TCCGATTTGG AACTGAGTCC CGGCCCATCC GCATCATTGT CGCTTGGCAA TTTGCCGCAG 
TTGGTTAAAG CGAGCTCAGA TCCATCGATT GCCACTAATC AGGATAACTT GGATAGGGAT 
AGAGACATAA TTGGTGAAGG ACTACCACCT CCATATACGG TACCCTACGA CCATGGTGTC 
CCCGCGAATC CCAATCGCCG CCAGACCATG GACTCCAGCA AGTACAGCAT CTACGGCACC 
AACGTGCCGC CGCAGCCGCA GCAGCCCGGC GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA 
CAGTCTCTGT ACGGGATTAA TGCACCCGAT CTGCCGCCCC GCATCGATCG TCAGTCCAAG 
CCGGGTGATA TGCCACTGAA CACCTCGGGC TCGTCGTCGC GAAATGGCAC CCTGGGTCGC 
AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT GTGGTGCAGG ACGATGTCCA GGCGGAATAC 
ATAACCCGAA ATGCTCTGGT CGGCTCCGGG GCGGCTGAAA CCCTGGATCG CCAGCAGCAG 
CAGCAAACCC ACGCGTCCTT GGAGCGGCAG GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC 
GGAGGAGGCG CCGGAGGAGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC 
AACAACACAC AAATGGCCAT GCAAAACCTG GGCCGACTGG GTCCCAATGC ACCCGACGAT 
CTCAAATCAG TGCCAAATGC AAGTGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCTCGAC 
TACGGACGCA CTCCGCACGA TCACCGCAGC TTCGGAGGAG CCAACGATCT GAACCGACAG 
AGCAGTCCGG GCAGACCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT TGATCCACGC 
AATGGCACTC CACAGCGTCC TTCGAATCTG GGCCTAGAAA GCAGTCCACG CAAGCCTTTG 
GTGGAGACGA AGACGGATTA CGGCAAATAC AGACGCTATC ATTTACACGC GCCCCATTTG 
AAGCACCTGC ATATGATGCA CTCGCTCTCA CCTTCGCCCT CGTCCTTGCC CCTTCACATG 
TCCTGCGGCC TTCAGCTGAC GCAGCAGCAG CTGCTGCGCC TCTCCGCCGG CGGCTTCGAT 
CGCCGCCCCA TTGCTGCACC ACGTCTGCAT GCCCTGGTGG CACCACTGCC ACCTCCAGCA 
CTGATGTCCA GTGCCTCGGC CTACGACACA CTCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026837740.1
CDS	652..5430
Translation	MTREEEEEVRSLLSRHQERIMLDLGSTDLLAVLVKNSVLSQSEEDLLLKPYNAVTPTPSAAASATVEPPGSAPSLTKRKLSAVASSGSTASGGSAGSGGSGSSRCASVNGDANCDSRSLTPSGGGPCSVLNDAEILRVQCSNLIEIIAKNGFEKFKQFCYAIECECPQLIEDLINDRLKTDAANLEDSRDGEKMQKEIETIDYIDKEKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYRRYHLHAPHLKHLHMMHSLSPSPSSLPLHMSCGLQLTQQQLLRLSAGGFDRRPIAAPRLHALVAPLPPPALMSSASAYDTLV

Target ORF information:

RefSeq Version	XM_026981939.1
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026981939.1

ORF Insert Sequence:

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCAGCTC GACGCACCGA 
CGCTCGAAGA GTCTGTCCCG CGAGAACTGG GACGACGTGG TCTTCTCCGA CAGCATTTCC 
AAATTCCCCG CCTACGAGCG AGTCGTTCTC CGCCATCCTG GCTTTGTTCG ACCCGTAGTG 
CTCTTCGGAC CCGTATCCGA TCTGGCCCGC GAGCGCTTGG CCAAGGATTT CCCCGACAAG 
TTTTCGACTC CACTGCAGGA TGATGACAAG TCAGCTGCAA CCAGTGGAAA GTGCCGCATC 
GTAAGGCTCT CGAACATACG CGATGTGATG GATCGTGGCA AGCACGCCCT GCTGGACATA 
ACGCCCAATG CAGTGGATCG TCTCAACTAC GCCCAGTTCT ATCCGGTGGT GATCTTCCTG 
AAGACAGACA GCAAGCACGT GATCAAGCAG CTGCGCCACG GACTACCCAA GGCGGCCCAC 
AAGAGCTCCA AGAAGCTGCT GGAGCAGACC CAGAAACTGG AGCGCGTCTG GTCCCACATC 
TTTAGCACCC AGATCGCGTT GAGCGACGAG GAGTCCTGGT ACCGAAAGTT GCGCGACTCG 
ATCGATCTGC AGCAGAGCGG CGCCGTGTGG ATGTCCGAGT CCAAGCCAGT AGAATCCCTC 
TCCGACGATT TCCTATTCCC CATGACCACA TCCCGACTCT CGTATGCATC AAGTCCCGAA 
TCCGATTTGG AACTGAGTCC CGGCCCATCC GCATCATTGT CGCTTGGCAA TTTGCCGCAG 
TTGGTTAAAG CGAGCTCAGA TCCATCGATT GCCACTAATC AGGATAACTT GGATAGGGAT 
AGAGACATAA TTGGTGAAGG ACTACCACCT CCATATACGG TACCCTACGA CCATGGTGTC 
CCCGCGAATC CCAATCGCCG CCAGACCATG GACTCCAGCA AGTACAGCAT CTACGGCACC 
AACGTGCCGC CGCAGCCGCA GCAGCCCGGC GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA 
CAGTCTCTGT ACGGGATTAA TGCACCCGAT CTGCCGCCCC GCATCGATCG TCAGTCCAAG 
CCGGGTGATA TGCCACTGAA CACCTCGGGC TCGTCGTCGC GAAATGGCAC CCTGGGTCGC 
AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT GTGGTGCAGG ACGATGTCCA GGCGGAATAC 
ATAACCCGAA ATGCTCTGGT CGGCTCCGGG GCGGCTGAAA CCCTGGATCG CCAGCAGCAG 
CAGCAAACCC ACGCGTCCTT GGAGCGGCAG GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC 
GGAGGAGGCG CCGGAGGAGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC 
AACAACACAC AAATGGCCAT GCAAAACCTG GGCCGACTGG GTCCCAATGC ACCCGACGAT 
CTCAAATCAG TGCCAAATGC AAGTGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCTCGAC 
TACGGACGCA CTCCGCACGA TCACCGCAGC TTCGGAGGAG CCAACGATCT GAACCGACAG 
AGCAGTCCGG GCAGACCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT TGATCCACGC 
AATGGCACTC CACAGCGTCC TTCGAATCTG GGCCTAGAAA GCAGTCCACG CAAGCCTTTG 
GTGGAGACGA AGACGGATTA CGGCAAATAC AGACGCTATC ATTTACACGC GCCCCATTTG 
AAGCACCTGC ATATGATGCA CTCGCTCTCA CCTTCGCCCT CGTCCTTGCC CCTTCACATG 
TCCTGCGGCC TTCAGCTGAC GCAGCAGCAG CTGCTGCGCC TCTCCGCCGG CGGCTTCGAT 
CGCCGCCCCA TTGCTGCACC ACGTCTGCAT GCCCTGGTGG CACCACTGCC ACCTCCAGCA 
CTGATGTCCA GTGCCTCGGC CTACGACACA CTCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_026981945.1 ) cDNA ORF clone, Drosophila erecta -> XP_026837746.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X11, mRNA.

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CloneID	ODi216958
Clone ID Related Accession (Same CDS sequence)	XM_026981945.1
Accession Version	XM_026981945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCAGCTC GACGCACCGA 
CGCTCGAAGA GTCTGTCCCG CGAGAACTGG GACGACGTGG TCTTCTCCGA CAGCATTTCC 
AAATTCCCCG CCTACGAGCG AGTCGTTCTC CGCCATCCTG GCTTTGTTCG ACCCGTAGTG 
CTCTTCGGAC CCGTATCCGA TCTGGCCCGC GAGCGCTTGG CCAAGGATTT CCCCGACAAG 
TTTTCGACTC CACTGCAGGA TGATGACAAG TCAGCTGCAA CCAGTGGAAA GTGCCGCATC 
GTAAGGCTCT CGAACATACG CGATGTGATG GATCGTGGCA AGCACGCCCT GCTGGACATA 
ACGCCCAATG CAGTGGATCG TCTCAACTAC GCCCAGTTCT ATCCGGTGGT GATCTTCCTG 
AAGACAGACA GCAAGCACGT GATCAAGCAG CTGCGCCACG GACTACCCAA GGCGGCCCAC 
AAGAGCTCCA AGAAGCTGCT GGAGCAGACC CAGAAACTGG AGCGCGTCTG GTCCCACATC 
TTTAGCACCC AGATCGCGTT GAGCGACGAG GAGTCCTGGT ACCGAAAGTT GCGCGACTCG 
ATCGATCTGC AGCAGAGCGG CGCCGTGTGG ATGTCCGAGT CCAAGTTCCC CGATTCCCCC 
ACCGAAATGT TATTCCCCCC CTACATCGCC CAGCCATGCA AGCTAGCTTG TTGCCGCCCC 
TTATCATCTA CCTCAGTGCC ACGCTACTCG CAGGATCTGA ATCTGAACCA GAATCCGAGT 
AAATCCTATT CGCTTATCCA AAACCAGGCG CACACCCCTC CGCCGCCACG CCCTCCGCCC 
GTGGGTTACG CCCACAGCCA GAGTTTGGCT GAAAAGCCCC GAGATCGGAG GTCCTATACG 
ATGGTGCCGA TCATAAAGCC CGCTTCTTCG ATGCCCAGTG GCCTGGCACC ACCCGTTGAG 
CCTGCTCCCA TGATGCCACT GGATGATGGT GTCCTGGCCA TGGATGTGTC CAACTCCCAG 
TATCACAGTT CTCAGCCAAA TCTAATGAGT CAGAGTTATT TGAACTCGAA CTTAAATGGC 
GGCAGTACGA GTGCCGTAAA TCAATCGAAC CAATCCAATG CCACGAGGGG CTTTCAACCC 
GGACCATCGA CAGTCAGCCG CTATGGATTG CGGTCCAATG CCCAGGTGGA ACGTAGGACC 
ACGCCCTACT ACTACCACGA GCTGCTGCTG CAGAAGAATC TCTTCGACTC CAGCCTGAAT 
CTTCTGCCCC AGCAGAGCAA GAACAGCAAT CTGCAGCTGG AGCAGCAGCA GCAGCAACAG 
CATCTTGCAG ATGCATCGAA TGCATCCAAA AGGGCACCGA TGGCCAAGCC ACAGCAGCGA 
TTGATTAGCA GTGGTCAGGG CAGAAGCACT TACTCCTCCT CGGGATCACT GAGCAATCTT 
CTGGGCAATG GCAATGGCAA TGGCAATGGG TTGAGCAATG GGCACGAGAG CAGCGATGGC 
ACTTTGGCGG ATGAGAGCAG CTTCACACTG GCCTTTAATA ATCTCACCAA TCAAATAGCC 
AGTCTCAACA ATTCAAGTAG CCTAAACAAC AATAATTGTA GCAACAGCAG GAGCATCAAC 
AACAACAGCA ACAGCCATAC TATCAATACC AACAATAATG TTAATAATGC TGATTCCAAC 
AACAATGTTG GCCAAAGTAA CGAATGCATT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026837746.1
CDS	652..5187
Translation	MTREEEEEVRSLLSRHQERIMLDLGSTDLLAVLVKNSVLSQSEEDLLLKPYNAVTPTPSAAASATVEPPGSAPSLTKRKLSAVASSGSTASGGSAGSGGSGSSRCASVNGDANCDSRSLTPSGGGPCSVLNDAEILRVQCSNLIEIIAKNGFEKFKQFCYAIECECPQLIEDLINDRLKTDAANLEDSRDGEKMQKEIETIDYIDKEKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKFPDSPTEMLFPPYIAQPCKLACCRPLSSTSVPRYSQDLNLNQNPSKSYSLIQNQAHTPPPPRPPPVGYAHSQSLAEKPRDRRSYTMVPIIKPASSMPSGLAPPVEPAPMMPLDDGVLAMDVSNSQYHSSQPNLMSQSYLNSNLNGGSTSAVNQSNQSNATRGFQPGPSTVSRYGLRSNAQVERRTTPYYYHELLLQKNLFDSSLNLLPQQSKNSNLQLEQQQQQQHLADASNASKRAPMAKPQQRLISSGQGRSTYSSSGSLSNLLGNGNGNGNGLSNGHESSDGTLADESSFTLAFNNLTNQIASLNNSSSLNNNNCSNSRSINNNSNSHTINTNNNVNNADSNNNVGQSNECIA

Target ORF information:

RefSeq Version	XM_026981945.1
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026981945.1

ORF Insert Sequence:

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCAGCTC GACGCACCGA 
CGCTCGAAGA GTCTGTCCCG CGAGAACTGG GACGACGTGG TCTTCTCCGA CAGCATTTCC 
AAATTCCCCG CCTACGAGCG AGTCGTTCTC CGCCATCCTG GCTTTGTTCG ACCCGTAGTG 
CTCTTCGGAC CCGTATCCGA TCTGGCCCGC GAGCGCTTGG CCAAGGATTT CCCCGACAAG 
TTTTCGACTC CACTGCAGGA TGATGACAAG TCAGCTGCAA CCAGTGGAAA GTGCCGCATC 
GTAAGGCTCT CGAACATACG CGATGTGATG GATCGTGGCA AGCACGCCCT GCTGGACATA 
ACGCCCAATG CAGTGGATCG TCTCAACTAC GCCCAGTTCT ATCCGGTGGT GATCTTCCTG 
AAGACAGACA GCAAGCACGT GATCAAGCAG CTGCGCCACG GACTACCCAA GGCGGCCCAC 
AAGAGCTCCA AGAAGCTGCT GGAGCAGACC CAGAAACTGG AGCGCGTCTG GTCCCACATC 
TTTAGCACCC AGATCGCGTT GAGCGACGAG GAGTCCTGGT ACCGAAAGTT GCGCGACTCG 
ATCGATCTGC AGCAGAGCGG CGCCGTGTGG ATGTCCGAGT CCAAGTTCCC CGATTCCCCC 
ACCGAAATGT TATTCCCCCC CTACATCGCC CAGCCATGCA AGCTAGCTTG TTGCCGCCCC 
TTATCATCTA CCTCAGTGCC ACGCTACTCG CAGGATCTGA ATCTGAACCA GAATCCGAGT 
AAATCCTATT CGCTTATCCA AAACCAGGCG CACACCCCTC CGCCGCCACG CCCTCCGCCC 
GTGGGTTACG CCCACAGCCA GAGTTTGGCT GAAAAGCCCC GAGATCGGAG GTCCTATACG 
ATGGTGCCGA TCATAAAGCC CGCTTCTTCG ATGCCCAGTG GCCTGGCACC ACCCGTTGAG 
CCTGCTCCCA TGATGCCACT GGATGATGGT GTCCTGGCCA TGGATGTGTC CAACTCCCAG 
TATCACAGTT CTCAGCCAAA TCTAATGAGT CAGAGTTATT TGAACTCGAA CTTAAATGGC 
GGCAGTACGA GTGCCGTAAA TCAATCGAAC CAATCCAATG CCACGAGGGG CTTTCAACCC 
GGACCATCGA CAGTCAGCCG CTATGGATTG CGGTCCAATG CCCAGGTGGA ACGTAGGACC 
ACGCCCTACT ACTACCACGA GCTGCTGCTG CAGAAGAATC TCTTCGACTC CAGCCTGAAT 
CTTCTGCCCC AGCAGAGCAA GAACAGCAAT CTGCAGCTGG AGCAGCAGCA GCAGCAACAG 
CATCTTGCAG ATGCATCGAA TGCATCCAAA AGGGCACCGA TGGCCAAGCC ACAGCAGCGA 
TTGATTAGCA GTGGTCAGGG CAGAAGCACT TACTCCTCCT CGGGATCACT GAGCAATCTT 
CTGGGCAATG GCAATGGCAA TGGCAATGGG TTGAGCAATG GGCACGAGAG CAGCGATGGC 
ACTTTGGCGG ATGAGAGCAG CTTCACACTG GCCTTTAATA ATCTCACCAA TCAAATAGCC 
AGTCTCAACA ATTCAAGTAG CCTAAACAAC AATAATTGTA GCAACAGCAG GAGCATCAAC 
AACAACAGCA ACAGCCATAC TATCAATACC AACAATAATG TTAATAATGC TGATTCCAAC 
AACAATGTTG GCCAAAGTAA CGAATGCATT GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_026981949.1 ) cDNA ORF clone, Drosophila erecta -> XP_026837750.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X12, mRNA.

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CloneID	ODi216959
Clone ID Related Accession (Same CDS sequence)	XM_026981949.1
Accession Version	XM_026981949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGTTCCCCGA TTCCCCCACC 
GAAATGTTAT TCCCCCCCTA CATCGCCCAG CCATGCAAGC TAGCTTGTTG CCGCCCCTTA 
TCATCTACCT CAGTGCCACG CTACTCGCAG GATCTGAATC TGAACCAGAA TCCGAGTAAA 
TCCTATTCGC TTATCCAAAA CCAGGCGCAC ACCCCTCCGC CGCCACGCCC TCCGCCCGTG 
GGTTACGCCC ACAGCCAGAG TTTGGCTGAA AAGCCCCGAG ATCGGAGGTC CTATACGATG 
GTGCCGATCA TAAAGCCCGC TTCTTCGATG CCCAGTGGCC TGGCACCACC CGTTGAGCCT 
GCTCCCATGA TGCCACTGGA TGATGGTGTC CTGGCCATGG ATGTGTCCAA CTCCCAGTAT 
CACAGTTCTC AGCCAAATCT AATGAGTCAG AGTTATTTGA ACTCGAACTT AAATGGCGGC 
AGTACGAGTG CCGTAAATCA ATCGAACCAA TCCAATGCCA CGAGGGGCTT TCAACCCGGA 
CCATCGACAG TCAGCCGCTA TGGATTGCGG TCCAATGCCC AGGTGGAACG TAGGACCACG 
CCCTACTACT ACCACGAGCT GCTGCTGCAG AAGAATCTCT TCGACTCCAG CCTGAATCTT 
CTGCCCCAGC AGAGCAAGAA CAGCAATCTG CAGCTGGAGC AGCAGCAGCA GCAACAGCAT 
CTTGCAGATG CATCGAATGC ATCCAAAAGG GCACCGATGG CCAAGCCACA GCAGCGATTG 
ATTAGCAGTG GTCAGGGCAG AAGCACTTAC TCCTCCTCGG GATCACTGAG CAATCTTCTG 
GGCAATGGCA ATGGCAATGG CAATGGGTTG AGCAATGGGC ACGAGAGCAG CGATGGCACT 
TTGGCGGATG AGAGCAGCTT CACACTGGCC TTTAATAATC TCACCAATCA AATAGCCAGT 
CTCAACAATT CAAGTAGCCT AAACAACAAT AATTGTAGCA ACAGCAGGAG CATCAACAAC 
AACAGCAACA GCCATACTAT CAATACCAAC AATAATGTTA ATAATGCTGA TTCCAACAAC 
AATGTTGGCC AAAGTAACGA ATGCATTGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026837750.1
CDS	652..5184
Translation	MTREEEEEVRSLLSRHQERIMLDLGSTDLLAVLVKNSVLSQSEEDLLLKPYNAVTPTPSAAASATVEPPGSAPSLTKRKLSAVASSGSTASGGSAGSGGSGSSRCASVNGDANCDSRSLTPSGGGPCSVLNDAEILRVQCSNLIEIIAKNGFEKFKQFCYAIECECPQLIEDLINDRLKTDAANLEDSRDGEKMQKEIETIDYIDKEKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKFPDSPTEMLFPPYIAQPCKLACCRPLSSTSVPRYSQDLNLNQNPSKSYSLIQNQAHTPPPPRPPPVGYAHSQSLAEKPRDRRSYTMVPIIKPASSMPSGLAPPVEPAPMMPLDDGVLAMDVSNSQYHSSQPNLMSQSYLNSNLNGGSTSAVNQSNQSNATRGFQPGPSTVSRYGLRSNAQVERRTTPYYYHELLLQKNLFDSSLNLLPQQSKNSNLQLEQQQQQQHLADASNASKRAPMAKPQQRLISSGQGRSTYSSSGSLSNLLGNGNGNGNGLSNGHESSDGTLADESSFTLAFNNLTNQIASLNNSSSLNNNNCSNSRSINNNSNSHTINTNNNVNNADSNNNVGQSNECIA

Target ORF information:

RefSeq Version	XM_026981949.1
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026981949.1

ORF Insert Sequence:

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGTTCCCCGA TTCCCCCACC 
GAAATGTTAT TCCCCCCCTA CATCGCCCAG CCATGCAAGC TAGCTTGTTG CCGCCCCTTA 
TCATCTACCT CAGTGCCACG CTACTCGCAG GATCTGAATC TGAACCAGAA TCCGAGTAAA 
TCCTATTCGC TTATCCAAAA CCAGGCGCAC ACCCCTCCGC CGCCACGCCC TCCGCCCGTG 
GGTTACGCCC ACAGCCAGAG TTTGGCTGAA AAGCCCCGAG ATCGGAGGTC CTATACGATG 
GTGCCGATCA TAAAGCCCGC TTCTTCGATG CCCAGTGGCC TGGCACCACC CGTTGAGCCT 
GCTCCCATGA TGCCACTGGA TGATGGTGTC CTGGCCATGG ATGTGTCCAA CTCCCAGTAT 
CACAGTTCTC AGCCAAATCT AATGAGTCAG AGTTATTTGA ACTCGAACTT AAATGGCGGC 
AGTACGAGTG CCGTAAATCA ATCGAACCAA TCCAATGCCA CGAGGGGCTT TCAACCCGGA 
CCATCGACAG TCAGCCGCTA TGGATTGCGG TCCAATGCCC AGGTGGAACG TAGGACCACG 
CCCTACTACT ACCACGAGCT GCTGCTGCAG AAGAATCTCT TCGACTCCAG CCTGAATCTT 
CTGCCCCAGC AGAGCAAGAA CAGCAATCTG CAGCTGGAGC AGCAGCAGCA GCAACAGCAT 
CTTGCAGATG CATCGAATGC ATCCAAAAGG GCACCGATGG CCAAGCCACA GCAGCGATTG 
ATTAGCAGTG GTCAGGGCAG AAGCACTTAC TCCTCCTCGG GATCACTGAG CAATCTTCTG 
GGCAATGGCA ATGGCAATGG CAATGGGTTG AGCAATGGGC ACGAGAGCAG CGATGGCACT 
TTGGCGGATG AGAGCAGCTT CACACTGGCC TTTAATAATC TCACCAATCA AATAGCCAGT 
CTCAACAATT CAAGTAGCCT AAACAACAAT AATTGTAGCA ACAGCAGGAG CATCAACAAC 
AACAGCAACA GCCATACTAT CAATACCAAC AATAATGTTA ATAATGCTGA TTCCAACAAC 
AATGTTGGCC AAAGTAACGA ATGCATTGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154547.1 ) cDNA ORF clone, Drosophila erecta -> XP_015010033.1 Drosophila erecta uncharacterized protein, transcript variant J (Dere\GG17413), mRNA.

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CloneID	ODi33934
Clone ID Related Accession (Same CDS sequence)	XM_015154547.1
Accession Version	XM_015154547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform J
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956552). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGCCAGTAGA ATCCCTCTCC 
GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC 
GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG 
GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA 
GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGGTGTCCCC 
GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAC 
GTGCCGCCGC AGCCGCAGCA GCCCGGCGCA GGAGGAGATC CGGCTGCAGT GAGGCCACAG 
TCTCTGTACG GGATTAATGC ACCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG 
GGTGATATGC CACTGAACAC CTCGGGCTCG TCGTCGCGAA ATGGCACCCT GGGTCGCAGT 
GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAATACATA 
ACCCGAAATG CTCTGGTCGG CTCCGGGGCG GCTGAAACCC TGGATCGCCA GCAGCAGCAG 
CAAACCCACG CGTCCTTGGA GCGGCAGGCG CGTTTGAATG CGCAGCTGAA GGCCAACGGA 
GGAGGCGCCG GAGGAGGTGC ATCCACCTAC GACAGTGTGT CCAGCTACGA CTCGTACAAC 
AACACACAAA TGGCCATGCA AAACCTGGGC CGACTGGGTC CCAATGCACC CGACGATCTC 
AAATCAGTGC CAAATGCAAG TGGTCGCCCC TTGCCTCCGA CGGGTCAGTC GCTCGACTAC 
GGACGCACTC CGCACGATCA CCGCAGCTTC GGAGGAGCCA ACGATCTGAA CCGACAGAGC 
AGTCCGGGCA GACCGCACTA CCATGACATG AATGCGTCCC GTAATATTGA TCCACGCAAT 
GGCACTCCAC AGCGTCCTTC GAATCTGGGC CTAGAAAGCA GTCCACGCAA GCCTTTGGTG 
GAGACGAAGA CGGATTACGG CAAATACAGA CGCTATCATT TACACGCGCC CCATTTGAAG 
CACCTGCATA TGATGCACTC GCTCTCACCT TCGCCCTCGT CCTTGCCCCT TCACATGTCC 
TGCGGCCTTC AGCTGACGCA GCAGCAGCTG CTGCGCCTCT CCGCCGGCGG CTTCGATCGC 
CGCCCCATTG CTGCACCACG TCTGCATGCC CTGGTGGCAC CACTGCCACC TCCAGCACTG 
ATGTCCAGTG CCTCGGCCTA CGACACACTC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010033.1
CDS	142..4917
Translation	MTREEEEEVRSLLSRHQERIMLDLGSTDLLAVLVKNSVLSQSEEDLLLKPYNAVTPTPSAAASATVEPPGSAPSLTKRKLSAVASSGSTASGGSAGSGGSGSSRCASVNGDANCDSRSLTPSGGGPCSVLNDAEILRVQCSNLIEIIAKNGFEKFKQFCYAIECECPQLIEDLINDRLKTDAANLEDSRDGEKMQKEIETIDYIDKEKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYRRYHLHAPHLKHLHMMHSLSPSPSSLPLHMSCGLQLTQQQLLRLSAGGFDRRPIAAPRLHALVAPLPPPALMSSASAYDTLV

Target ORF information:

RefSeq Version	XM_015154547.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant J (Dere\GG17413), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154547.1

ORF Insert Sequence:

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGCCAGTAGA ATCCCTCTCC 
GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC 
GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG 
GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA 
GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGGTGTCCCC 
GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAC 
GTGCCGCCGC AGCCGCAGCA GCCCGGCGCA GGAGGAGATC CGGCTGCAGT GAGGCCACAG 
TCTCTGTACG GGATTAATGC ACCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG 
GGTGATATGC CACTGAACAC CTCGGGCTCG TCGTCGCGAA ATGGCACCCT GGGTCGCAGT 
GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAATACATA 
ACCCGAAATG CTCTGGTCGG CTCCGGGGCG GCTGAAACCC TGGATCGCCA GCAGCAGCAG 
CAAACCCACG CGTCCTTGGA GCGGCAGGCG CGTTTGAATG CGCAGCTGAA GGCCAACGGA 
GGAGGCGCCG GAGGAGGTGC ATCCACCTAC GACAGTGTGT CCAGCTACGA CTCGTACAAC 
AACACACAAA TGGCCATGCA AAACCTGGGC CGACTGGGTC CCAATGCACC CGACGATCTC 
AAATCAGTGC CAAATGCAAG TGGTCGCCCC TTGCCTCCGA CGGGTCAGTC GCTCGACTAC 
GGACGCACTC CGCACGATCA CCGCAGCTTC GGAGGAGCCA ACGATCTGAA CCGACAGAGC 
AGTCCGGGCA GACCGCACTA CCATGACATG AATGCGTCCC GTAATATTGA TCCACGCAAT 
GGCACTCCAC AGCGTCCTTC GAATCTGGGC CTAGAAAGCA GTCCACGCAA GCCTTTGGTG 
GAGACGAAGA CGGATTACGG CAAATACAGA CGCTATCATT TACACGCGCC CCATTTGAAG 
CACCTGCATA TGATGCACTC GCTCTCACCT TCGCCCTCGT CCTTGCCCCT TCACATGTCC 
TGCGGCCTTC AGCTGACGCA GCAGCAGCTG CTGCGCCTCT CCGCCGGCGG CTTCGATCGC 
CGCCCCATTG CTGCACCACG TCTGCATGCC CTGGTGGCAC CACTGCCACC TCCAGCACTG 
ATGTCCAGTG CCTCGGCCTA CGACACACTC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154548.2 ) cDNA ORF clone, Drosophila erecta -> XP_015010034.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODi33935
Clone ID Related Accession (Same CDS sequence)	XM_015154548.2 , XM_015154548.1
Accession Version	XM_015154548.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015154548.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGCCAGTAGA ATCCCTCTCC 
GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC 
GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG 
GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA 
GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGGTGTCCCC 
GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAC 
GTGCCGCCGC AGCCGCAGCA GCCCGGCGCA GGAGGAGATC CGGCTGCAGT GAGGCCACAG 
TCTCTGTACG GGATTAATGC ACCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG 
GGTGATATGC CACTGAACAC CTCGGGCTCG TCGTCGCGAA ATGGCACCCT GGGTCGCAGT 
GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAATACATA 
ACCCGAAATG CTCTGGTCGG CTCCGGGGCG GCTGAAACCC TGGATCGCCA GCAGCAGCAG 
CAAACCCACG CGTCCTTGGA GCGGCAGGCG CGTTTGAATG CGCAGCTGAA GGCCAACGGA 
GGAGGCGCCG GAGGAGGTGC ATCCACCTAC GACAGTGTGT CCAGCTACGA CTCGTACAAC 
AACACACAAA TGGCCATGCA AAACCTGGGC CGACTGGGTC CCAATGCACC CGACGATCTC 
AAATCAGTGC CAAATGCAAG TGGTCGCCCC TTGCCTCCGA CGGGTCAGTC GCTCGACTAC 
GGACGCACTC CGCACGATCA CCGCAGCTTC GGAGGAGCCA ACGATCTGAA CCGACAGAGC 
AGTCCGGGCA GACCGCACTA CCATGACATG AATGCGTCCC GTAATATTGA TCCACGCAAT 
GGCACTCCAC AGCGTCCTTC GAATCTGGGC CTAGAAAGCA GTCCACGCAA GCCTTTGGTG 
GAGACGAAGA CGGATTACGG CAAATACAGT CGAAACAACT CCGTATCGCA AGCGGATTAC 
ACCAAGCTAC CGAAGACGGC GCCACATGGA GTGGTACCGC CGCCGAATGT GAGCAACGGC 
CAAAGCCAGA TGAATGGCAG TGGAACGCCC AGCAGCAATG GCAGTGGACC CTTCAAGCCG 
GTGCCGCCAC CCAAACCCAA GAACTATAGG CCACCGGTGC AAAGTGGTGG CAGCAGTGGC 
AGCGGTGGCA CTACTCCGTG GGAAAATGGG GACTCTGGCT CGCCACGCTC TCCAAATGGC 
TTCTATTATC CACCAACGCC TTCGCATCAT CACTACGGCC AGCAGGCGAC TCCCGGTTCA 
CCCAGCAACG GCCACATGCA GCCACCTCCG CCGCAGCAGC AGCAGCAGCA GCCCACCTAC 
GGCGGAAGTA ATGGCAACTA CGGACAGGCG CCACCACCAC AACCCTATCC GCAGGCCAAT 
GGCTACAACG GGAATGGCCA TCACTACAAC GGAGGCAGCG GCACGGGACC CTACATTGCA 
CCACATCGCG GCATGCCGCC GCCAATAGGA AACCTGCCGC CCCACACGCC CGAACGTCAT 
GCCCTGGACT TGGCCGGAAG TCGGGAGCAG CGTGGCTCGG CCTTCGAGCT GTATCGTAAG 
CCGCAAATCG GAGCAACCGC TGGGCACCAT CACAACATGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010034.1
CDS	652..5856
Translation	MTREEEEEVRSLLSRHQERIMLDLGSTDLLAVLVKNSVLSQSEEDLLLKPYNAVTPTPSAAASATVEPPGSAPSLTKRKLSAVASSGSTASGGSAGSGGSGSSRCASVNGDANCDSRSLTPSGGGPCSVLNDAEILRVQCSNLIEIIAKNGFEKFKQFCYAIECECPQLIEDLINDRLKTDAANLEDSRDGEKMQKEIETIDYIDKEKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMR

Target ORF information:

RefSeq Version	XM_015154548.2
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154548.2

ORF Insert Sequence:

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGCCAGTAGA ATCCCTCTCC 
GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC 
GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG 
GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA 
GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGGTGTCCCC 
GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAC 
GTGCCGCCGC AGCCGCAGCA GCCCGGCGCA GGAGGAGATC CGGCTGCAGT GAGGCCACAG 
TCTCTGTACG GGATTAATGC ACCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG 
GGTGATATGC CACTGAACAC CTCGGGCTCG TCGTCGCGAA ATGGCACCCT GGGTCGCAGT 
GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAATACATA 
ACCCGAAATG CTCTGGTCGG CTCCGGGGCG GCTGAAACCC TGGATCGCCA GCAGCAGCAG 
CAAACCCACG CGTCCTTGGA GCGGCAGGCG CGTTTGAATG CGCAGCTGAA GGCCAACGGA 
GGAGGCGCCG GAGGAGGTGC ATCCACCTAC GACAGTGTGT CCAGCTACGA CTCGTACAAC 
AACACACAAA TGGCCATGCA AAACCTGGGC CGACTGGGTC CCAATGCACC CGACGATCTC 
AAATCAGTGC CAAATGCAAG TGGTCGCCCC TTGCCTCCGA CGGGTCAGTC GCTCGACTAC 
GGACGCACTC CGCACGATCA CCGCAGCTTC GGAGGAGCCA ACGATCTGAA CCGACAGAGC 
AGTCCGGGCA GACCGCACTA CCATGACATG AATGCGTCCC GTAATATTGA TCCACGCAAT 
GGCACTCCAC AGCGTCCTTC GAATCTGGGC CTAGAAAGCA GTCCACGCAA GCCTTTGGTG 
GAGACGAAGA CGGATTACGG CAAATACAGT CGAAACAACT CCGTATCGCA AGCGGATTAC 
ACCAAGCTAC CGAAGACGGC GCCACATGGA GTGGTACCGC CGCCGAATGT GAGCAACGGC 
CAAAGCCAGA TGAATGGCAG TGGAACGCCC AGCAGCAATG GCAGTGGACC CTTCAAGCCG 
GTGCCGCCAC CCAAACCCAA GAACTATAGG CCACCGGTGC AAAGTGGTGG CAGCAGTGGC 
AGCGGTGGCA CTACTCCGTG GGAAAATGGG GACTCTGGCT CGCCACGCTC TCCAAATGGC 
TTCTATTATC CACCAACGCC TTCGCATCAT CACTACGGCC AGCAGGCGAC TCCCGGTTCA 
CCCAGCAACG GCCACATGCA GCCACCTCCG CCGCAGCAGC AGCAGCAGCA GCCCACCTAC 
GGCGGAAGTA ATGGCAACTA CGGACAGGCG CCACCACCAC AACCCTATCC GCAGGCCAAT 
GGCTACAACG GGAATGGCCA TCACTACAAC GGAGGCAGCG GCACGGGACC CTACATTGCA 
CCACATCGCG GCATGCCGCC GCCAATAGGA AACCTGCCGC CCCACACGCC CGAACGTCAT 
GCCCTGGACT TGGCCGGAAG TCGGGAGCAG CGTGGCTCGG CCTTCGAGCT GTATCGTAAG 
CCGCAAATCG GAGCAACCGC TGGGCACCAT CACAACATGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154548.1 ) cDNA ORF clone, Drosophila erecta -> XP_015010034.1 Drosophila erecta uncharacterized protein, transcript variant K (Dere\GG17413), mRNA.

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CloneID	ODi33935
Clone ID Related Accession (Same CDS sequence)	XM_015154548.2 , XM_015154548.1
Accession Version	XM_015154548.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform K
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956552). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGCCAGTAGA ATCCCTCTCC 
GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC 
GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG 
GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA 
GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGGTGTCCCC 
GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAC 
GTGCCGCCGC AGCCGCAGCA GCCCGGCGCA GGAGGAGATC CGGCTGCAGT GAGGCCACAG 
TCTCTGTACG GGATTAATGC ACCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG 
GGTGATATGC CACTGAACAC CTCGGGCTCG TCGTCGCGAA ATGGCACCCT GGGTCGCAGT 
GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAATACATA 
ACCCGAAATG CTCTGGTCGG CTCCGGGGCG GCTGAAACCC TGGATCGCCA GCAGCAGCAG 
CAAACCCACG CGTCCTTGGA GCGGCAGGCG CGTTTGAATG CGCAGCTGAA GGCCAACGGA 
GGAGGCGCCG GAGGAGGTGC ATCCACCTAC GACAGTGTGT CCAGCTACGA CTCGTACAAC 
AACACACAAA TGGCCATGCA AAACCTGGGC CGACTGGGTC CCAATGCACC CGACGATCTC 
AAATCAGTGC CAAATGCAAG TGGTCGCCCC TTGCCTCCGA CGGGTCAGTC GCTCGACTAC 
GGACGCACTC CGCACGATCA CCGCAGCTTC GGAGGAGCCA ACGATCTGAA CCGACAGAGC 
AGTCCGGGCA GACCGCACTA CCATGACATG AATGCGTCCC GTAATATTGA TCCACGCAAT 
GGCACTCCAC AGCGTCCTTC GAATCTGGGC CTAGAAAGCA GTCCACGCAA GCCTTTGGTG 
GAGACGAAGA CGGATTACGG CAAATACAGT CGAAACAACT CCGTATCGCA AGCGGATTAC 
ACCAAGCTAC CGAAGACGGC GCCACATGGA GTGGTACCGC CGCCGAATGT GAGCAACGGC 
CAAAGCCAGA TGAATGGCAG TGGAACGCCC AGCAGCAATG GCAGTGGACC CTTCAAGCCG 
GTGCCGCCAC CCAAACCCAA GAACTATAGG CCACCGGTGC AAAGTGGTGG CAGCAGTGGC 
AGCGGTGGCA CTACTCCGTG GGAAAATGGG GACTCTGGCT CGCCACGCTC TCCAAATGGC 
TTCTATTATC CACCAACGCC TTCGCATCAT CACTACGGCC AGCAGGCGAC TCCCGGTTCA 
CCCAGCAACG GCCACATGCA GCCACCTCCG CCGCAGCAGC AGCAGCAGCA GCCCACCTAC 
GGCGGAAGTA ATGGCAACTA CGGACAGGCG CCACCACCAC AACCCTATCC GCAGGCCAAT 
GGCTACAACG GGAATGGCCA TCACTACAAC GGAGGCAGCG GCACGGGACC CTACATTGCA 
CCACATCGCG GCATGCCGCC GCCAATAGGA AACCTGCCGC CCCACACGCC CGAACGTCAT 
GCCCTGGACT TGGCCGGAAG TCGGGAGCAG CGTGGCTCGG CCTTCGAGCT GTATCGTAAG 
CCGCAAATCG GAGCAACCGC TGGGCACCAT CACAACATGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010034.1
CDS	142..5346
Translation	MTREEEEEVRSLLSRHQERIMLDLGSTDLLAVLVKNSVLSQSEEDLLLKPYNAVTPTPSAAASATVEPPGSAPSLTKRKLSAVASSGSTASGGSAGSGGSGSSRCASVNGDANCDSRSLTPSGGGPCSVLNDAEILRVQCSNLIEIIAKNGFEKFKQFCYAIECECPQLIEDLINDRLKTDAANLEDSRDGEKMQKEIETIDYIDKEKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMR

Target ORF information:

RefSeq Version	XM_015154548.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant K (Dere\GG17413), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154548.1

ORF Insert Sequence:

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGCCAGTAGA ATCCCTCTCC 
GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC 
GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG 
GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA 
GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGGTGTCCCC 
GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAC 
GTGCCGCCGC AGCCGCAGCA GCCCGGCGCA GGAGGAGATC CGGCTGCAGT GAGGCCACAG 
TCTCTGTACG GGATTAATGC ACCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG 
GGTGATATGC CACTGAACAC CTCGGGCTCG TCGTCGCGAA ATGGCACCCT GGGTCGCAGT 
GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAATACATA 
ACCCGAAATG CTCTGGTCGG CTCCGGGGCG GCTGAAACCC TGGATCGCCA GCAGCAGCAG 
CAAACCCACG CGTCCTTGGA GCGGCAGGCG CGTTTGAATG CGCAGCTGAA GGCCAACGGA 
GGAGGCGCCG GAGGAGGTGC ATCCACCTAC GACAGTGTGT CCAGCTACGA CTCGTACAAC 
AACACACAAA TGGCCATGCA AAACCTGGGC CGACTGGGTC CCAATGCACC CGACGATCTC 
AAATCAGTGC CAAATGCAAG TGGTCGCCCC TTGCCTCCGA CGGGTCAGTC GCTCGACTAC 
GGACGCACTC CGCACGATCA CCGCAGCTTC GGAGGAGCCA ACGATCTGAA CCGACAGAGC 
AGTCCGGGCA GACCGCACTA CCATGACATG AATGCGTCCC GTAATATTGA TCCACGCAAT 
GGCACTCCAC AGCGTCCTTC GAATCTGGGC CTAGAAAGCA GTCCACGCAA GCCTTTGGTG 
GAGACGAAGA CGGATTACGG CAAATACAGT CGAAACAACT CCGTATCGCA AGCGGATTAC 
ACCAAGCTAC CGAAGACGGC GCCACATGGA GTGGTACCGC CGCCGAATGT GAGCAACGGC 
CAAAGCCAGA TGAATGGCAG TGGAACGCCC AGCAGCAATG GCAGTGGACC CTTCAAGCCG 
GTGCCGCCAC CCAAACCCAA GAACTATAGG CCACCGGTGC AAAGTGGTGG CAGCAGTGGC 
AGCGGTGGCA CTACTCCGTG GGAAAATGGG GACTCTGGCT CGCCACGCTC TCCAAATGGC 
TTCTATTATC CACCAACGCC TTCGCATCAT CACTACGGCC AGCAGGCGAC TCCCGGTTCA 
CCCAGCAACG GCCACATGCA GCCACCTCCG CCGCAGCAGC AGCAGCAGCA GCCCACCTAC 
GGCGGAAGTA ATGGCAACTA CGGACAGGCG CCACCACCAC AACCCTATCC GCAGGCCAAT 
GGCTACAACG GGAATGGCCA TCACTACAAC GGAGGCAGCG GCACGGGACC CTACATTGCA 
CCACATCGCG GCATGCCGCC GCCAATAGGA AACCTGCCGC CCCACACGCC CGAACGTCAT 
GCCCTGGACT TGGCCGGAAG TCGGGAGCAG CGTGGCTCGG CCTTCGAGCT GTATCGTAAG 
CCGCAAATCG GAGCAACCGC TGGGCACCAT CACAACATGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_026981907.1 ) cDNA ORF clone, Drosophila erecta -> XP_026837708.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X1, mRNA.

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CloneID	ODi216952
Clone ID Related Accession (Same CDS sequence)	XM_026981907.1
Accession Version	XM_026981907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCAGCTC GACGCACCGA 
CGCTCGAAGA GTCTGTCCCG CGAGAACTGG GACGACGTGG TCTTCTCCGA CAGCATTTCC 
AAATTCCCCG CCTACGAGCG AGTCGTTCTC CGCCATCCTG GCTTTGTTCG ACCCGTAGTG 
CTCTTCGGAC CCGTATCCGA TCTGGCCCGC GAGCGCTTGG CCAAGGATTT CCCCGACAAG 
TTTTCGACTC CACTGCAGGA TGATGACAAG TCAGCTGCAA CCAGTGGAAA GTGCCGCATC 
GTAAGGCTCT CGAACATACG CGATGTGATG GATCGTGGCA AGCACGCCCT GCTGGACATA 
ACGCCCAATG CAGTGGATCG TCTCAACTAC GCCCAGTTCT ATCCGGTGGT GATCTTCCTG 
AAGACAGACA GCAAGCACGT GATCAAGCAG CTGCGCCACG GACTACCCAA GGCGGCCCAC 
AAGAGCTCCA AGAAGCTGCT GGAGCAGACC CAGAAACTGG AGCGCGTCTG GTCCCACATC 
TTTAGCACCC AGATCGCGTT GAGCGACGAG GAGTCCTGGT ACCGAAAGTT GCGCGACTCG 
ATCGATCTGC AGCAGAGCGG CGCCGTGTGG ATGTCCGAGT CCAAGCCAGT AGAATCCCTC 
TCCGACGATT TCCTATTCCC CATGACCACA TCCCGACTCT CGTATGCATC AAGTCCCGAA 
TCCGATTTGG AACTGAGTCC CGGCCCATCC GCATCATTGT CGCTTGGCAA TTTGCCGCAG 
TTGGTTAAAG CGAGCTCAGA TCCATCGATT GCCACTAATC AGGATAACTT GGATAGGGAT 
AGAGACATAA TTGGTGAAGG ACTACCACCT CCATATACGG TACCCTACGA CCATGGTGTC 
CCCGCGAATC CCAATCGCCG CCAGACCATG GACTCCAGCA AGTACAGCAT CTACGGCACC 
AACGTGCCGC CGCAGCCGCA GCAGCCCGGC GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA 
CAGTCTCTGT ACGGGATTAA TGCACCCGAT CTGCCGCCCC GCATCGATCG TCAGTCCAAG 
CCGGGTGATA TGCCACTGAA CACCTCGGGC TCGTCGTCGC GAAATGGCAC CCTGGGTCGC 
AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT GTGGTGCAGG ACGATGTCCA GGCGGAATAC 
ATAACCCGAA ATGCTCTGGT CGGCTCCGGG GCGGCTGAAA CCCTGGATCG CCAGCAGCAG 
CAGCAAACCC ACGCGTCCTT GGAGCGGCAG GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC 
GGAGGAGGCG CCGGAGGAGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC 
AACAACACAC AAATGGCCAT GCAAAACCTG GGCCGACTGG GTCCCAATGC ACCCGACGAT 
CTCAAATCAG TGCCAAATGC AAGTGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCTCGAC 
TACGGACGCA CTCCGCACGA TCACCGCAGC TTCGGAGGAG CCAACGATCT GAACCGACAG 
AGCAGTCCGG GCAGACCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT TGATCCACGC 
AATGGCACTC CACAGCGTCC TTCGAATCTG GGCCTAGAAA GCAGTCCACG CAAGCCTTTG 
GTGGAGACGA AGACGGATTA CGGCAAATAC AGTCGAAACA ACTCCGTATC GCAAGCGGAT 
TACACCAAGC TACCGAAGAC GGCGCCACAT GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC 
GGCCAAAGCC AGATGAATGG CAGTGGAACG CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG 
CCGGTGCCGC CACCCAAACC CAAGAACTAT AGGCCACCGG TGCAAAGTGG TGGCAGCAGT 
GGCAGCGGTG GCACTACTCC GTGGGAAAAT GGGGACTCTG GCTCGCCACG CTCTCCAAAT 
GGCTTCTATT ATCCACCAAC GCCTTCGCAT CATCACTACG GCCAGCAGGC GACTCCCGGT 
TCACCCAGCA ACGGCCACAT GCAGCCACCT CCGCCGCAGC AGCAGCAGCA GCAGCCCACC 
TACGGCGGAA GTAATGGCAA CTACGGACAG GCGCCACCAC CACAACCCTA TCCGCAGGCC 
AATGGCTACA ACGGGAATGG CCATCACTAC AACGGAGGCA GCGGCACGGG ACCCTACATT 
GCACCACATC GCGGCATGCC GCCGCCAATA GGAAACCTGC CGCCCCACAC GCCCGAACGT 
CATGCCCTGG ACTTGGCCGG AAGTCGGGAG CAGCGTGGCT CGGCCTTCGA GCTGTATCGT 
AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC CATCACAACA TGAGCGAAAT GGAGCCGTAC 
GACGAGCGAT ACGATGATTA CTATAACATG CCACCCCCTG CCGCCCATCC ATCCCAAGGG 
CACCATATGC AGCGCTCCCG ATCAGCTCCT CGTTATCCCC AGGAGCGACC TCCTCACGCA 
CAAGATCCGA ATTACTACGG CCACTATGGA ACTTCTAGGG GACATAGTCA GCCGCGCCAG 
CAGTATCCTC AGCATCACCA GCAACAGTAT TACGATGAGC ATGGCATGGA GATGGGTCCA 
CCGCCACTGC CCCCACATAA GAAGAAGAAA TCGGTGCTGA AGAGCCCACT GGTGGCCCTC 
AAGAATGCGC TGCTTAAGAG CACGCGGCCC CTGAGGAGAA TGAACAGCAT GGTGGAGCCG 
GAGAGGAAAC CGAAGGGACT CCGACGCCAG CAATCGATGC TGGAGAGGGG TGTGCAAAGA 
CCCTACTATC CGGATGAGTA TCCAACCTAT CCAGCTGGCT TTGAGGAGCG TGCCCATGGC 
GGACAAGGCA TGATGCAGCC TCATCCGCAA GATGCCTACT ATCAAAGGGG TGGTCACTAC 
ACGTCGCAGC AGCAGGAGAT GATGAACAGC ACCTACCAGA ATCTGGAGGG TGAGGATATC 
TATGGGAACA TCGGAAACAC AGTTCCTAGA ATGCCGCATC ACCAGGACAA TGGTTACGGA 
TATGACCAAT ACGATCTCTA TGCCAATCGA GCGTGCATCG ATCTCGAAAG AAGGCAAGCA 
GAGGCAGCTG CAGCTTCCGG TGGCAGAAAT GGGAGGAGAA TTGTGAGGCG GCACAGCACC 
ACCACTGCGG ATCGAGGAGG AAATCCCGGA CCGCCCAGGA GGCCTATGAT CAGTCCTGGT 
TATGAGCAGG ATCCGCAAGA GATCTACCAG ACCCGCAACG GAGCCTACAT GCTGCCCGAT 
CAGCGAAGGG CGCCCAGTTC GGAGGTGATG ACCCGCAGAA GATTCTATCC GGGGGCTGCC 
AATGAGCAAA CTGAGGAGGA GCCCCTCTAC CAATCCCGTC GCGAAATGCA GCGTGAGATG 
CAGAGGAATC ATCTGTACCA ATCGAAACGG GAAATGCAGG AGAGGATTAG CCAGGGCAAG 
AGGGATATGG AGCGAGAGTT TAGCCCCCAG GGCAGCAGCA GCCAGTCGGA GACCTCCAAT 
CCGGAGGTAA TATACCAGAG TCGCCGGGAG GCCAAGGAAA GTGCTCTGAA AACGAGGGCA 
CAACTGAGGG ATCAGATCTA TCAGACTAGA CGGGAAGCCC TGGACAGCAT GGCGGAACCG 
ATCTATGTGT CCAAAAGAGA TATGGGCAGA CCGGCTCCTA TCTATGAGAC TCGGGAAGAG 
AGTATCCTCC AGTCCCGAGA AAATGAAACC GACGAAAAGA AGGAAAAGGA GTGCAAGACC 
GAGCAGATTC AGGTGGAGAT TAATGCTCAA CCGGAGGAAC AGGTGGAAGA TTCGACCTTA 
AGTCGATCCG ACTTGCAGAA ATCTTCGGAC ACGGTGATAG AAAATCCAGC GAGAGCTCCT 
CTTGTGCAGG ATGAGGTAGA TGACGACGAA GAAGAGCAGG CTCAGGCTCG GGATGATCCC 
GCTGAAAGCT CTGCGGATCA GACAGAGCTG CCAACGGCCA TTGAAAACAA TCTACACAAC 
GAGGGAATGG TGGAGCAAAC ACAGAATGAG TCGGATGACG TATTCGAAGC AGCCGATAAG 
GTTTCTCCTC TAGCCGCTCC TACAGGTACT GTTCAACCGC CGACCCCGCG ATCCAGTAGA 
GCTCCCTTCC ACATTTCGAA TATCCTAAAG CGAACAGCAC CTCCTCCGAG TTCCCCAATG 
GGCGACAGCT GCACCTCCAT CGAAACCCAG TACACATCCC AGGCCAGCCT GCCAGTGGGC 
CCACCAAATG CCACCAGTAC GCCGTTCAGT AGCAGCATGT CATTGCCCAT CGCCGGACCC 
GTAAACAATC AACCGCCGGC CAGCGGACCC TTTCCCACTC TGCCCCGCGA ACCCAGCACC 
TCGCGCGGAT TCTTTGACTC CAATGGTGGC ACTCTGGCAG ATAAGCTGTG GCACGTCTCA 
CTGCAAATCC CGCCGGGAGC CATTCCAGCC GGAGTACGGC AAGAGATCTA CTTCACCGTG 
AGCGATCCGC GAATGGGACA AGCCGTCGGC GGTCCTCCGC TGGACATGGA AAATGGTGAG 
ACAATGCTGT CACCGCTGGT GATGTGCGGG CCGCAGGGCC TTGAGTTCCT GGTACCGGTT 
ACACTCAACA TACCGCACTG CGCCGGACGA ACTGCATCAC TGGGACTGGC CCTCAAGGCG 
ACCGACAGCG AGAAGAACCT GCACACCGAG TGGGACAACA TCGACCTGCC CAGCAATGCC 
GCCGCCCACA CGGTCTCCGT CAAGGTGGAT CACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026837708.1
CDS	652..8250
Translation	MTREEEEEVRSLLSRHQERIMLDLGSTDLLAVLVKNSVLSQSEEDLLLKPYNAVTPTPSAAASATVEPPGSAPSLTKRKLSAVASSGSTASGGSAGSGGSGSSRCASVNGDANCDSRSLTPSGGGPCSVLNDAEILRVQCSNLIEIIAKNGFEKFKQFCYAIECECPQLIEDLINDRLKTDAANLEDSRDGEKMQKEIETIDYIDKEKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_026981907.1
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026981907.1

ORF Insert Sequence:

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCAGCTC GACGCACCGA 
CGCTCGAAGA GTCTGTCCCG CGAGAACTGG GACGACGTGG TCTTCTCCGA CAGCATTTCC 
AAATTCCCCG CCTACGAGCG AGTCGTTCTC CGCCATCCTG GCTTTGTTCG ACCCGTAGTG 
CTCTTCGGAC CCGTATCCGA TCTGGCCCGC GAGCGCTTGG CCAAGGATTT CCCCGACAAG 
TTTTCGACTC CACTGCAGGA TGATGACAAG TCAGCTGCAA CCAGTGGAAA GTGCCGCATC 
GTAAGGCTCT CGAACATACG CGATGTGATG GATCGTGGCA AGCACGCCCT GCTGGACATA 
ACGCCCAATG CAGTGGATCG TCTCAACTAC GCCCAGTTCT ATCCGGTGGT GATCTTCCTG 
AAGACAGACA GCAAGCACGT GATCAAGCAG CTGCGCCACG GACTACCCAA GGCGGCCCAC 
AAGAGCTCCA AGAAGCTGCT GGAGCAGACC CAGAAACTGG AGCGCGTCTG GTCCCACATC 
TTTAGCACCC AGATCGCGTT GAGCGACGAG GAGTCCTGGT ACCGAAAGTT GCGCGACTCG 
ATCGATCTGC AGCAGAGCGG CGCCGTGTGG ATGTCCGAGT CCAAGCCAGT AGAATCCCTC 
TCCGACGATT TCCTATTCCC CATGACCACA TCCCGACTCT CGTATGCATC AAGTCCCGAA 
TCCGATTTGG AACTGAGTCC CGGCCCATCC GCATCATTGT CGCTTGGCAA TTTGCCGCAG 
TTGGTTAAAG CGAGCTCAGA TCCATCGATT GCCACTAATC AGGATAACTT GGATAGGGAT 
AGAGACATAA TTGGTGAAGG ACTACCACCT CCATATACGG TACCCTACGA CCATGGTGTC 
CCCGCGAATC CCAATCGCCG CCAGACCATG GACTCCAGCA AGTACAGCAT CTACGGCACC 
AACGTGCCGC CGCAGCCGCA GCAGCCCGGC GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA 
CAGTCTCTGT ACGGGATTAA TGCACCCGAT CTGCCGCCCC GCATCGATCG TCAGTCCAAG 
CCGGGTGATA TGCCACTGAA CACCTCGGGC TCGTCGTCGC GAAATGGCAC CCTGGGTCGC 
AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT GTGGTGCAGG ACGATGTCCA GGCGGAATAC 
ATAACCCGAA ATGCTCTGGT CGGCTCCGGG GCGGCTGAAA CCCTGGATCG CCAGCAGCAG 
CAGCAAACCC ACGCGTCCTT GGAGCGGCAG GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC 
GGAGGAGGCG CCGGAGGAGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC 
AACAACACAC AAATGGCCAT GCAAAACCTG GGCCGACTGG GTCCCAATGC ACCCGACGAT 
CTCAAATCAG TGCCAAATGC AAGTGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCTCGAC 
TACGGACGCA CTCCGCACGA TCACCGCAGC TTCGGAGGAG CCAACGATCT GAACCGACAG 
AGCAGTCCGG GCAGACCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT TGATCCACGC 
AATGGCACTC CACAGCGTCC TTCGAATCTG GGCCTAGAAA GCAGTCCACG CAAGCCTTTG 
GTGGAGACGA AGACGGATTA CGGCAAATAC AGTCGAAACA ACTCCGTATC GCAAGCGGAT 
TACACCAAGC TACCGAAGAC GGCGCCACAT GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC 
GGCCAAAGCC AGATGAATGG CAGTGGAACG CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG 
CCGGTGCCGC CACCCAAACC CAAGAACTAT AGGCCACCGG TGCAAAGTGG TGGCAGCAGT 
GGCAGCGGTG GCACTACTCC GTGGGAAAAT GGGGACTCTG GCTCGCCACG CTCTCCAAAT 
GGCTTCTATT ATCCACCAAC GCCTTCGCAT CATCACTACG GCCAGCAGGC GACTCCCGGT 
TCACCCAGCA ACGGCCACAT GCAGCCACCT CCGCCGCAGC AGCAGCAGCA GCAGCCCACC 
TACGGCGGAA GTAATGGCAA CTACGGACAG GCGCCACCAC CACAACCCTA TCCGCAGGCC 
AATGGCTACA ACGGGAATGG CCATCACTAC AACGGAGGCA GCGGCACGGG ACCCTACATT 
GCACCACATC GCGGCATGCC GCCGCCAATA GGAAACCTGC CGCCCCACAC GCCCGAACGT 
CATGCCCTGG ACTTGGCCGG AAGTCGGGAG CAGCGTGGCT CGGCCTTCGA GCTGTATCGT 
AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC CATCACAACA TGAGCGAAAT GGAGCCGTAC 
GACGAGCGAT ACGATGATTA CTATAACATG CCACCCCCTG CCGCCCATCC ATCCCAAGGG 
CACCATATGC AGCGCTCCCG ATCAGCTCCT CGTTATCCCC AGGAGCGACC TCCTCACGCA 
CAAGATCCGA ATTACTACGG CCACTATGGA ACTTCTAGGG GACATAGTCA GCCGCGCCAG 
CAGTATCCTC AGCATCACCA GCAACAGTAT TACGATGAGC ATGGCATGGA GATGGGTCCA 
CCGCCACTGC CCCCACATAA GAAGAAGAAA TCGGTGCTGA AGAGCCCACT GGTGGCCCTC 
AAGAATGCGC TGCTTAAGAG CACGCGGCCC CTGAGGAGAA TGAACAGCAT GGTGGAGCCG 
GAGAGGAAAC CGAAGGGACT CCGACGCCAG CAATCGATGC TGGAGAGGGG TGTGCAAAGA 
CCCTACTATC CGGATGAGTA TCCAACCTAT CCAGCTGGCT TTGAGGAGCG TGCCCATGGC 
GGACAAGGCA TGATGCAGCC TCATCCGCAA GATGCCTACT ATCAAAGGGG TGGTCACTAC 
ACGTCGCAGC AGCAGGAGAT GATGAACAGC ACCTACCAGA ATCTGGAGGG TGAGGATATC 
TATGGGAACA TCGGAAACAC AGTTCCTAGA ATGCCGCATC ACCAGGACAA TGGTTACGGA 
TATGACCAAT ACGATCTCTA TGCCAATCGA GCGTGCATCG ATCTCGAAAG AAGGCAAGCA 
GAGGCAGCTG CAGCTTCCGG TGGCAGAAAT GGGAGGAGAA TTGTGAGGCG GCACAGCACC 
ACCACTGCGG ATCGAGGAGG AAATCCCGGA CCGCCCAGGA GGCCTATGAT CAGTCCTGGT 
TATGAGCAGG ATCCGCAAGA GATCTACCAG ACCCGCAACG GAGCCTACAT GCTGCCCGAT 
CAGCGAAGGG CGCCCAGTTC GGAGGTGATG ACCCGCAGAA GATTCTATCC GGGGGCTGCC 
AATGAGCAAA CTGAGGAGGA GCCCCTCTAC CAATCCCGTC GCGAAATGCA GCGTGAGATG 
CAGAGGAATC ATCTGTACCA ATCGAAACGG GAAATGCAGG AGAGGATTAG CCAGGGCAAG 
AGGGATATGG AGCGAGAGTT TAGCCCCCAG GGCAGCAGCA GCCAGTCGGA GACCTCCAAT 
CCGGAGGTAA TATACCAGAG TCGCCGGGAG GCCAAGGAAA GTGCTCTGAA AACGAGGGCA 
CAACTGAGGG ATCAGATCTA TCAGACTAGA CGGGAAGCCC TGGACAGCAT GGCGGAACCG 
ATCTATGTGT CCAAAAGAGA TATGGGCAGA CCGGCTCCTA TCTATGAGAC TCGGGAAGAG 
AGTATCCTCC AGTCCCGAGA AAATGAAACC GACGAAAAGA AGGAAAAGGA GTGCAAGACC 
GAGCAGATTC AGGTGGAGAT TAATGCTCAA CCGGAGGAAC AGGTGGAAGA TTCGACCTTA 
AGTCGATCCG ACTTGCAGAA ATCTTCGGAC ACGGTGATAG AAAATCCAGC GAGAGCTCCT 
CTTGTGCAGG ATGAGGTAGA TGACGACGAA GAAGAGCAGG CTCAGGCTCG GGATGATCCC 
GCTGAAAGCT CTGCGGATCA GACAGAGCTG CCAACGGCCA TTGAAAACAA TCTACACAAC 
GAGGGAATGG TGGAGCAAAC ACAGAATGAG TCGGATGACG TATTCGAAGC AGCCGATAAG 
GTTTCTCCTC TAGCCGCTCC TACAGGTACT GTTCAACCGC CGACCCCGCG ATCCAGTAGA 
GCTCCCTTCC ACATTTCGAA TATCCTAAAG CGAACAGCAC CTCCTCCGAG TTCCCCAATG 
GGCGACAGCT GCACCTCCAT CGAAACCCAG TACACATCCC AGGCCAGCCT GCCAGTGGGC 
CCACCAAATG CCACCAGTAC GCCGTTCAGT AGCAGCATGT CATTGCCCAT CGCCGGACCC 
GTAAACAATC AACCGCCGGC CAGCGGACCC TTTCCCACTC TGCCCCGCGA ACCCAGCACC 
TCGCGCGGAT TCTTTGACTC CAATGGTGGC ACTCTGGCAG ATAAGCTGTG GCACGTCTCA 
CTGCAAATCC CGCCGGGAGC CATTCCAGCC GGAGTACGGC AAGAGATCTA CTTCACCGTG 
AGCGATCCGC GAATGGGACA AGCCGTCGGC GGTCCTCCGC TGGACATGGA AAATGGTGAG 
ACAATGCTGT CACCGCTGGT GATGTGCGGG CCGCAGGGCC TTGAGTTCCT GGTACCGGTT 
ACACTCAACA TACCGCACTG CGCCGGACGA ACTGCATCAC TGGGACTGGC CCTCAAGGCG 
ACCGACAGCG AGAAGAACCT GCACACCGAG TGGGACAACA TCGACCTGCC CAGCAATGCC 
GCCGCCCACA CGGTCTCCGT CAAGGTGGAT CACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_026981909.1 ) cDNA ORF clone, Drosophila erecta -> XP_026837710.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X3, mRNA.

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CloneID	ODi216953
Clone ID Related Accession (Same CDS sequence)	XM_026981909.1
Accession Version	XM_026981909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGATC CACCGCCACC GCCGCCACCC AAGCCGCAAT TGGGCTCCAA AGCGGATTCC 
GTAGCGCATT TGGCCAGCAA ATATCCCCAA TCCGAGTACA ATCTCATACA GAAAATCGAC 
TCGAACAGCA CACTCACTGC TCCGGCGCAA TATCAACCGC AGAATTTCTA CGCGAATACC 
GGAACCATAT CCTCCACGAA TGGCTATGGA TCGCTGTTGT GTCACGCCAG TTCGACGACC 
AGTCCGCTGG CGGTTCGGAA GCGGGACAAG CTGCTCCATC GGTTCAGCGA TGCCGCCACC 
TTGGGCCGGA AGCTCAAGAA GAAGAAGAAC ACGAATCGCA CCTGCAGATC CATGACGGAG 
GCTATTGAGA TGCTGGCCGA TCCCGTCATC GAGGATGAAT TCTTTGGTGA TCGAACCACT 
TGGGAGTACC ACACGGTGGC AGTGACTCGG GTGCCGGGCT ATGGATTCGG CATCGCCGTT 
TCCGGTGGAC GTGATAATCC GCACTTCGCC AACGGCGATC CCTCGATAGC AGTTAGCGAT 
GTGCTGAAAG GTGGCCCCGC CGAGGATCGA TTGCAAGTCA ACGATCGGAT AATCTCGGTG 
AACGGCGTCT CCCTGGAGAA CGTGGAATAT GCCACCGCGG TGCAGGTGTT GCGCGATAGT 
GGCAACACGG TCCAATTGGT GGTCAAGCGT CGCGTGCCCC TCAATCCCAT CAATAATGGA 
GGTGCAGTGC AGCATCAGCA CTCGCACAGT CTCAGCTCGG TTGGTCTATT GGCCAATGGA 
AGTGGAGGAG TTGCACCAAC GCCCTTGACG AGCCTGAGTC AACCCAACTC CCTGAACTCT 
TCGCTGGTGC AGAATGCCAG CAGTGGTCAG CCCATCAAGG TGACCCTCAC GAAAGGCGGC 
AAGAAAGATG ACTATGGCGT GGTCCTTGGC TGCCGGCTGT TTGTTAAGGA GATCTCCTCC 
AAAGCTCGCG AGCAACTTAA TGCCAATGGC TACAGTTTGC AAGAGGGTGA CATTATCACC 
CGCATCCACA ACACCAATTG CGGGGATACG ATGAGTCTCA AGGAGGCGAA AAAGATCATC 
GATGGTTGCA AGGAGCGCTT GAACCTAGTG GTTCTACGGG ACATCACCAA CCAAGCAGCT 
GTTAGCCAAC TGAATCTGAA CAATTCAGCG AGCCACCAGG CTTCGGCAAA TATATACGGC 
ACTCATCAGC CTCAGGTATC CGGATGTAGC AGCAGTAACA ACAATCTTGA AGATCCATAT 
CTGCCGGGCG GAGCCAGTTA CTCCTCGCAG AATCTGTACG TGCAACCACC CACTCGCACC 
TCCAATGGTC CCAATCTCAA TGGCAATGGG CTGAACGACG AAAAGAGTAA TCTCACACCG 
CGAGGTCGCT CCCGGGGGCC CATTATGGAT GGGGTATCTC TGCAGCAGCT GGACAGGCCC 
GTAACACCGA CGCGTGGCAG GAGTGCGGCC ATCGACGAGC CGCCACGTCC ACCGCCGCCA 
CGAGGATCGA GCGGGGGAGC GGCCCAGGAG GATTTCTACA GCTCTCGCAG GCAATTGTAC 
GAGGAGCGCC AGAGTGCCGA GCCGAGATTC ATTTCCTTCC AGAAGGAGGG AAGCGTGGGC 
ATCCGCCTAA CCGGCGGCAA TGAAGCCGGT ATTTTTGTGA CTGCCGTACA ACCGGGATCT 
CCAGCCTCGC TGCAGGGTCT AATGCCAGGC GATAAGATCC TGAAGGTCAA CGACATGGAC 
ATGAACGGAG TGACGCGCGA GGAGGCTGTT CTCTTTCTGT TGAGCCTGCA GGATCGCATC 
GATCTGATTG TGCAGTACTG CAAGGAGGAG TACGACGAGG TGGTGACCAA CCAACGTGGC 
GACTCCTTCC ACATCAAGAC ACATTTCCAC TGCGATAATC CCTCCAAAGG CGAGATGGCC 
TTCAAGGCGG GCGATGTGTT CCGGGTGATC GATACCCTGC ACAATGGTGT AGTCGGTTCC 
TGGCAGGTGC TGAAGATCGG ACGTGGTCAC CAGGAGATGC AGCGCGGCGT TATACCGAAC 
AAGTCGCGGG CCGAAGAACT GGCCACTGCC CAGTTCAATG CCACCAAGAA GGAGATGAAC 
GCCAACGAGT CGAGGGGCAA CTTCTTCCGG CGACGACGCA GCTCGACGCA CCGACGCTCG 
AAGAGTCTGT CCCGCGAGAA CTGGGACGAC GTGGTCTTCT CCGACAGCAT TTCCAAATTC 
CCCGCCTACG AGCGAGTCGT TCTCCGCCAT CCTGGCTTTG TTCGACCCGT AGTGCTCTTC 
GGACCCGTAT CCGATCTGGC CCGCGAGCGC TTGGCCAAGG ATTTCCCCGA CAAGTTTTCG 
ACTCCACTGC AGGATGATGA CAAGTCAGCT GCAACCAGTG GAAAGTGCCG CATCGTAAGG 
CTCTCGAACA TACGCGATGT GATGGATCGT GGCAAGCACG CCCTGCTGGA CATAACGCCC 
AATGCAGTGG ATCGTCTCAA CTACGCCCAG TTCTATCCGG TGGTGATCTT CCTGAAGACA 
GACAGCAAGC ACGTGATCAA GCAGCTGCGC CACGGACTAC CCAAGGCGGC CCACAAGAGC 
TCCAAGAAGC TGCTGGAGCA GACCCAGAAA CTGGAGCGCG TCTGGTCCCA CATCTTTAGC 
ACCCAGATCG CGTTGAGCGA CGAGGAGTCC TGGTACCGAA AGTTGCGCGA CTCGATCGAT 
CTGCAGCAGA GCGGCGCCGT GTGGATGTCC GAGTCCAAGC CAGTAGAATC CCTCTCCGAC 
GATTTCCTAT TCCCCATGAC CACATCCCGA CTCTCGTATG CATCAAGTCC CGAATCCGAT 
TTGGAACTGA GTCCCGGCCC ATCCGCATCA TTGTCGCTTG GCAATTTGCC GCAGTTGGTT 
AAAGCGAGCT CAGATCCATC GATTGCCACT AATCAGGATA ACTTGGATAG GGATAGAGAC 
ATAATTGGTG AAGGACTACC ACCTCCATAT ACGGTACCCT ACGACCATGG TGTCCCCGCG 
AATCCCAATC GCCGCCAGAC CATGGACTCC AGCAAGTACA GCATCTACGG CACCAACGTG 
CCGCCGCAGC CGCAGCAGCC CGGCGCAGGA GGAGATCCGG CTGCAGTGAG GCCACAGTCT 
CTGTACGGGA TTAATGCACC CGATCTGCCG CCCCGCATCG ATCGTCAGTC CAAGCCGGGT 
GATATGCCAC TGAACACCTC GGGCTCGTCG TCGCGAAATG GCACCCTGGG TCGCAGTGCC 
CAGGAGCGTC TGTTTGGCAA GGCTGTGGTG CAGGACGATG TCCAGGCGGA ATACATAACC 
CGAAATGCTC TGGTCGGCTC CGGGGCGGCT GAAACCCTGG ATCGCCAGCA GCAGCAGCAA 
ACCCACGCGT CCTTGGAGCG GCAGGCGCGT TTGAATGCGC AGCTGAAGGC CAACGGAGGA 
GGCGCCGGAG GAGGTGCATC CACCTACGAC AGTGTGTCCA GCTACGACTC GTACAACAAC 
ACACAAATGG CCATGCAAAA CCTGGGCCGA CTGGGTCCCA ATGCACCCGA CGATCTCAAA 
TCAGTGCCAA ATGCAAGTGG TCGCCCCTTG CCTCCGACGG GTCAGTCGCT CGACTACGGA 
CGCACTCCGC ACGATCACCG CAGCTTCGGA GGAGCCAACG ATCTGAACCG ACAGAGCAGT 
CCGGGCAGAC CGCACTACCA TGACATGAAT GCGTCCCGTA ATATTGATCC ACGCAATGGC 
ACTCCACAGC GTCCTTCGAA TCTGGGCCTA GAAAGCAGTC CACGCAAGCC TTTGGTGGAG 
ACGAAGACGG ATTACGGCAA ATACAGTCGA AACAACTCCG TATCGCAAGC GGATTACACC 
AAGCTACCGA AGACGGCGCC ACATGGAGTG GTACCGCCGC CGAATGTGAG CAACGGCCAA 
AGCCAGATGA ATGGCAGTGG AACGCCCAGC AGCAATGGCA GTGGACCCTT CAAGCCGGTG 
CCGCCACCCA AACCCAAGAA CTATAGGCCA CCGGTGCAAA GTGGTGGCAG CAGTGGCAGC 
GGTGGCACTA CTCCGTGGGA AAATGGGGAC TCTGGCTCGC CACGCTCTCC AAATGGCTTC 
TATTATCCAC CAACGCCTTC GCATCATCAC TACGGCCAGC AGGCGACTCC CGGTTCACCC 
AGCAACGGCC ACATGCAGCC ACCTCCGCCG CAGCAGCAGC AGCAGCAGCC CACCTACGGC 
GGAAGTAATG GCAACTACGG ACAGGCGCCA CCACCACAAC CCTATCCGCA GGCCAATGGC 
TACAACGGGA ATGGCCATCA CTACAACGGA GGCAGCGGCA CGGGACCCTA CATTGCACCA 
CATCGCGGCA TGCCGCCGCC AATAGGAAAC CTGCCGCCCC ACACGCCCGA ACGTCATGCC 
CTGGACTTGG CCGGAAGTCG GGAGCAGCGT GGCTCGGCCT TCGAGCTGTA TCGTAAGCCG 
CAAATCGGAG CAACCGCTGG GCACCATCAC AACATGAGCG AAATGGAGCC GTACGACGAG 
CGATACGATG ATTACTATAA CATGCCACCC CCTGCCGCCC ATCCATCCCA AGGGCACCAT 
ATGCAGCGCT CCCGATCAGC TCCTCGTTAT CCCCAGGAGC GACCTCCTCA CGCACAAGAT 
CCGAATTACT ACGGCCACTA TGGAACTTCT AGGGGACATA GTCAGCCGCG CCAGCAGTAT 
CCTCAGCATC ACCAGCAACA GTATTACGAT GAGCATGGCA TGGAGATGGG TCCACCGCCA 
CTGCCCCCAC ATAAGAAGAA GAAATCGGTG CTGAAGAGCC CACTGGTGGC CCTCAAGAAT 
GCGCTGCTTA AGAGCACGCG GCCCCTGAGG AGAATGAACA GCATGGTGGA GCCGGAGAGG 
AAACCGAAGG GACTCCGACG CCAGCAATCG ATGCTGGAGA GGGGTGTGCA AAGACCCTAC 
TATCCGGATG AGTATCCAAC CTATCCAGCT GGCTTTGAGG AGCGTGCCCA TGGCGGACAA 
GGCATGATGC AGCCTCATCC GCAAGATGCC TACTATCAAA GGGGTGGTCA CTACACGTCG 
CAGCAGCAGG AGATGATGAA CAGCACCTAC CAGAATCTGG AGGGTGAGGA TATCTATGGG 
AACATCGGAA ACACAGTTCC TAGAATGCCG CATCACCAGG ACAATGGTTA CGGATATGAC 
CAATACGATC TCTATGCCAA TCGAGCGTGC ATCGATCTCG AAAGAAGGCA AGCAGAGGCA 
GCTGCAGCTT CCGGTGGCAG AAATGGGAGG AGAATTGTGA GGCGGCACAG CACCACCACT 
GCGGATCGAG GAGGAAATCC CGGACCGCCC AGGAGGCCTA TGATCAGTCC TGGTTATGAG 
CAGGATCCGC AAGAGATCTA CCAGACCCGC AACGGAGCCT ACATGCTGCC CGATCAGCGA 
AGGGCGCCCA GTTCGGAGGT GATGACCCGC AGAAGATTCT ATCCGGGGGC TGCCAATGAG 
CAAACTGAGG AGGAGCCCCT CTACCAATCC CGTCGCGAAA TGCAGCGTGA GATGCAGAGG 
AATCATCTGT ACCAATCGAA ACGGGAAATG CAGGAGAGGA TTAGCCAGGG CAAGAGGGAT 
ATGGAGCGAG AGTTTAGCCC CCAGGGCAGC AGCAGCCAGT CGGAGACCTC CAATCCGGAG 
GTAATATACC AGAGTCGCCG GGAGGCCAAG GAAAGTGCTC TGAAAACGAG GGCACAACTG 
AGGGATCAGA TCTATCAGAC TAGACGGGAA GCCCTGGACA GCATGGCGGA ACCGATCTAT 
GTGTCCAAAA GAGATATGGG CAGACCGGCT CCTATCTATG AGACTCGGGA AGAGAGTATC 
CTCCAGTCCC GAGAAAATGA AACCGACGAA AAGAAGGAAA AGGAGTGCAA GACCGAGCAG 
ATTCAGGTGG AGATTAATGC TCAACCGGAG GAACAGGTGG AAGATTCGAC CTTAAGTCGA 
TCCGACTTGC AGAAATCTTC GGACACGGTG ATAGAAAATC CAGCGAGAGC TCCTCTTGTG 
CAGGATGAGG TAGATGACGA CGAAGAAGAG CAGGCTCAGG CTCGGGATGA TCCCGCTGAA 
AGCTCTGCGG ATCAGACAGA GCTGCCAACG GCCATTGAAA ACAATCTACA CAACGAGGGA 
ATGGTGGAGC AAACACAGAA TGAGTCGGAT GACGTATTCG AAGCAGCCGA TAAGGTTTCT 
CCTCTAGCCG CTCCTACAGG TACTGTTCAA CCGCCGACCC CGCGATCCAG TAGAGCTCCC 
TTCCACATTT CGAATATCCT AAAGCGAACA GCACCTCCTC CGAGTTCCCC AATGGGCGAC 
AGCTGCACCT CCATCGAAAC CCAGTACACA TCCCAGGCCA GCCTGCCAGT GGGCCCACCA 
AATGCCACCA GTACGCCGTT CAGTAGCAGC ATGTCATTGC CCATCGCCGG ACCCGTAAAC 
AATCAACCGC CGGCCAGCGG ACCCTTTCCC ACTCTGCCCC GCGAACCCAG CACCTCGCGC 
GGATTCTTTG ACTCCAATGG TGGCACTCTG GCAGATAAGC TGTGGCACGT CTCACTGCAA 
ATCCCGCCGG GAGCCATTCC AGCCGGAGTA CGGCAAGAGA TCTACTTCAC CGTGAGCGAT 
CCGCGAATGG GACAAGCCGT CGGCGGTCCT CCGCTGGACA TGGAAAATGG TGAGACAATG 
CTGTCACCGC TGGTGATGTG CGGGCCGCAG GGCCTTGAGT TCCTGGTACC GGTTACACTC 
AACATACCGC ACTGCGCCGG ACGAACTGCA TCACTGGGAC TGGCCCTCAA GGCGACCGAC 
AGCGAGAAGA ACCTGCACAC CGAGTGGGAC AACATCGACC TGCCCAGCAA TGCCGCCGCC 
CACACGGTCT CCGTCAAGGT GGATCACTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026837710.1
CDS	127..6999
Translation	MKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_026981909.1
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026981909.1

ORF Insert Sequence:

ATGAAGGATC CACCGCCACC GCCGCCACCC AAGCCGCAAT TGGGCTCCAA AGCGGATTCC 
GTAGCGCATT TGGCCAGCAA ATATCCCCAA TCCGAGTACA ATCTCATACA GAAAATCGAC 
TCGAACAGCA CACTCACTGC TCCGGCGCAA TATCAACCGC AGAATTTCTA CGCGAATACC 
GGAACCATAT CCTCCACGAA TGGCTATGGA TCGCTGTTGT GTCACGCCAG TTCGACGACC 
AGTCCGCTGG CGGTTCGGAA GCGGGACAAG CTGCTCCATC GGTTCAGCGA TGCCGCCACC 
TTGGGCCGGA AGCTCAAGAA GAAGAAGAAC ACGAATCGCA CCTGCAGATC CATGACGGAG 
GCTATTGAGA TGCTGGCCGA TCCCGTCATC GAGGATGAAT TCTTTGGTGA TCGAACCACT 
TGGGAGTACC ACACGGTGGC AGTGACTCGG GTGCCGGGCT ATGGATTCGG CATCGCCGTT 
TCCGGTGGAC GTGATAATCC GCACTTCGCC AACGGCGATC CCTCGATAGC AGTTAGCGAT 
GTGCTGAAAG GTGGCCCCGC CGAGGATCGA TTGCAAGTCA ACGATCGGAT AATCTCGGTG 
AACGGCGTCT CCCTGGAGAA CGTGGAATAT GCCACCGCGG TGCAGGTGTT GCGCGATAGT 
GGCAACACGG TCCAATTGGT GGTCAAGCGT CGCGTGCCCC TCAATCCCAT CAATAATGGA 
GGTGCAGTGC AGCATCAGCA CTCGCACAGT CTCAGCTCGG TTGGTCTATT GGCCAATGGA 
AGTGGAGGAG TTGCACCAAC GCCCTTGACG AGCCTGAGTC AACCCAACTC CCTGAACTCT 
TCGCTGGTGC AGAATGCCAG CAGTGGTCAG CCCATCAAGG TGACCCTCAC GAAAGGCGGC 
AAGAAAGATG ACTATGGCGT GGTCCTTGGC TGCCGGCTGT TTGTTAAGGA GATCTCCTCC 
AAAGCTCGCG AGCAACTTAA TGCCAATGGC TACAGTTTGC AAGAGGGTGA CATTATCACC 
CGCATCCACA ACACCAATTG CGGGGATACG ATGAGTCTCA AGGAGGCGAA AAAGATCATC 
GATGGTTGCA AGGAGCGCTT GAACCTAGTG GTTCTACGGG ACATCACCAA CCAAGCAGCT 
GTTAGCCAAC TGAATCTGAA CAATTCAGCG AGCCACCAGG CTTCGGCAAA TATATACGGC 
ACTCATCAGC CTCAGGTATC CGGATGTAGC AGCAGTAACA ACAATCTTGA AGATCCATAT 
CTGCCGGGCG GAGCCAGTTA CTCCTCGCAG AATCTGTACG TGCAACCACC CACTCGCACC 
TCCAATGGTC CCAATCTCAA TGGCAATGGG CTGAACGACG AAAAGAGTAA TCTCACACCG 
CGAGGTCGCT CCCGGGGGCC CATTATGGAT GGGGTATCTC TGCAGCAGCT GGACAGGCCC 
GTAACACCGA CGCGTGGCAG GAGTGCGGCC ATCGACGAGC CGCCACGTCC ACCGCCGCCA 
CGAGGATCGA GCGGGGGAGC GGCCCAGGAG GATTTCTACA GCTCTCGCAG GCAATTGTAC 
GAGGAGCGCC AGAGTGCCGA GCCGAGATTC ATTTCCTTCC AGAAGGAGGG AAGCGTGGGC 
ATCCGCCTAA CCGGCGGCAA TGAAGCCGGT ATTTTTGTGA CTGCCGTACA ACCGGGATCT 
CCAGCCTCGC TGCAGGGTCT AATGCCAGGC GATAAGATCC TGAAGGTCAA CGACATGGAC 
ATGAACGGAG TGACGCGCGA GGAGGCTGTT CTCTTTCTGT TGAGCCTGCA GGATCGCATC 
GATCTGATTG TGCAGTACTG CAAGGAGGAG TACGACGAGG TGGTGACCAA CCAACGTGGC 
GACTCCTTCC ACATCAAGAC ACATTTCCAC TGCGATAATC CCTCCAAAGG CGAGATGGCC 
TTCAAGGCGG GCGATGTGTT CCGGGTGATC GATACCCTGC ACAATGGTGT AGTCGGTTCC 
TGGCAGGTGC TGAAGATCGG ACGTGGTCAC CAGGAGATGC AGCGCGGCGT TATACCGAAC 
AAGTCGCGGG CCGAAGAACT GGCCACTGCC CAGTTCAATG CCACCAAGAA GGAGATGAAC 
GCCAACGAGT CGAGGGGCAA CTTCTTCCGG CGACGACGCA GCTCGACGCA CCGACGCTCG 
AAGAGTCTGT CCCGCGAGAA CTGGGACGAC GTGGTCTTCT CCGACAGCAT TTCCAAATTC 
CCCGCCTACG AGCGAGTCGT TCTCCGCCAT CCTGGCTTTG TTCGACCCGT AGTGCTCTTC 
GGACCCGTAT CCGATCTGGC CCGCGAGCGC TTGGCCAAGG ATTTCCCCGA CAAGTTTTCG 
ACTCCACTGC AGGATGATGA CAAGTCAGCT GCAACCAGTG GAAAGTGCCG CATCGTAAGG 
CTCTCGAACA TACGCGATGT GATGGATCGT GGCAAGCACG CCCTGCTGGA CATAACGCCC 
AATGCAGTGG ATCGTCTCAA CTACGCCCAG TTCTATCCGG TGGTGATCTT CCTGAAGACA 
GACAGCAAGC ACGTGATCAA GCAGCTGCGC CACGGACTAC CCAAGGCGGC CCACAAGAGC 
TCCAAGAAGC TGCTGGAGCA GACCCAGAAA CTGGAGCGCG TCTGGTCCCA CATCTTTAGC 
ACCCAGATCG CGTTGAGCGA CGAGGAGTCC TGGTACCGAA AGTTGCGCGA CTCGATCGAT 
CTGCAGCAGA GCGGCGCCGT GTGGATGTCC GAGTCCAAGC CAGTAGAATC CCTCTCCGAC 
GATTTCCTAT TCCCCATGAC CACATCCCGA CTCTCGTATG CATCAAGTCC CGAATCCGAT 
TTGGAACTGA GTCCCGGCCC ATCCGCATCA TTGTCGCTTG GCAATTTGCC GCAGTTGGTT 
AAAGCGAGCT CAGATCCATC GATTGCCACT AATCAGGATA ACTTGGATAG GGATAGAGAC 
ATAATTGGTG AAGGACTACC ACCTCCATAT ACGGTACCCT ACGACCATGG TGTCCCCGCG 
AATCCCAATC GCCGCCAGAC CATGGACTCC AGCAAGTACA GCATCTACGG CACCAACGTG 
CCGCCGCAGC CGCAGCAGCC CGGCGCAGGA GGAGATCCGG CTGCAGTGAG GCCACAGTCT 
CTGTACGGGA TTAATGCACC CGATCTGCCG CCCCGCATCG ATCGTCAGTC CAAGCCGGGT 
GATATGCCAC TGAACACCTC GGGCTCGTCG TCGCGAAATG GCACCCTGGG TCGCAGTGCC 
CAGGAGCGTC TGTTTGGCAA GGCTGTGGTG CAGGACGATG TCCAGGCGGA ATACATAACC 
CGAAATGCTC TGGTCGGCTC CGGGGCGGCT GAAACCCTGG ATCGCCAGCA GCAGCAGCAA 
ACCCACGCGT CCTTGGAGCG GCAGGCGCGT TTGAATGCGC AGCTGAAGGC CAACGGAGGA 
GGCGCCGGAG GAGGTGCATC CACCTACGAC AGTGTGTCCA GCTACGACTC GTACAACAAC 
ACACAAATGG CCATGCAAAA CCTGGGCCGA CTGGGTCCCA ATGCACCCGA CGATCTCAAA 
TCAGTGCCAA ATGCAAGTGG TCGCCCCTTG CCTCCGACGG GTCAGTCGCT CGACTACGGA 
CGCACTCCGC ACGATCACCG CAGCTTCGGA GGAGCCAACG ATCTGAACCG ACAGAGCAGT 
CCGGGCAGAC CGCACTACCA TGACATGAAT GCGTCCCGTA ATATTGATCC ACGCAATGGC 
ACTCCACAGC GTCCTTCGAA TCTGGGCCTA GAAAGCAGTC CACGCAAGCC TTTGGTGGAG 
ACGAAGACGG ATTACGGCAA ATACAGTCGA AACAACTCCG TATCGCAAGC GGATTACACC 
AAGCTACCGA AGACGGCGCC ACATGGAGTG GTACCGCCGC CGAATGTGAG CAACGGCCAA 
AGCCAGATGA ATGGCAGTGG AACGCCCAGC AGCAATGGCA GTGGACCCTT CAAGCCGGTG 
CCGCCACCCA AACCCAAGAA CTATAGGCCA CCGGTGCAAA GTGGTGGCAG CAGTGGCAGC 
GGTGGCACTA CTCCGTGGGA AAATGGGGAC TCTGGCTCGC CACGCTCTCC AAATGGCTTC 
TATTATCCAC CAACGCCTTC GCATCATCAC TACGGCCAGC AGGCGACTCC CGGTTCACCC 
AGCAACGGCC ACATGCAGCC ACCTCCGCCG CAGCAGCAGC AGCAGCAGCC CACCTACGGC 
GGAAGTAATG GCAACTACGG ACAGGCGCCA CCACCACAAC CCTATCCGCA GGCCAATGGC 
TACAACGGGA ATGGCCATCA CTACAACGGA GGCAGCGGCA CGGGACCCTA CATTGCACCA 
CATCGCGGCA TGCCGCCGCC AATAGGAAAC CTGCCGCCCC ACACGCCCGA ACGTCATGCC 
CTGGACTTGG CCGGAAGTCG GGAGCAGCGT GGCTCGGCCT TCGAGCTGTA TCGTAAGCCG 
CAAATCGGAG CAACCGCTGG GCACCATCAC AACATGAGCG AAATGGAGCC GTACGACGAG 
CGATACGATG ATTACTATAA CATGCCACCC CCTGCCGCCC ATCCATCCCA AGGGCACCAT 
ATGCAGCGCT CCCGATCAGC TCCTCGTTAT CCCCAGGAGC GACCTCCTCA CGCACAAGAT 
CCGAATTACT ACGGCCACTA TGGAACTTCT AGGGGACATA GTCAGCCGCG CCAGCAGTAT 
CCTCAGCATC ACCAGCAACA GTATTACGAT GAGCATGGCA TGGAGATGGG TCCACCGCCA 
CTGCCCCCAC ATAAGAAGAA GAAATCGGTG CTGAAGAGCC CACTGGTGGC CCTCAAGAAT 
GCGCTGCTTA AGAGCACGCG GCCCCTGAGG AGAATGAACA GCATGGTGGA GCCGGAGAGG 
AAACCGAAGG GACTCCGACG CCAGCAATCG ATGCTGGAGA GGGGTGTGCA AAGACCCTAC 
TATCCGGATG AGTATCCAAC CTATCCAGCT GGCTTTGAGG AGCGTGCCCA TGGCGGACAA 
GGCATGATGC AGCCTCATCC GCAAGATGCC TACTATCAAA GGGGTGGTCA CTACACGTCG 
CAGCAGCAGG AGATGATGAA CAGCACCTAC CAGAATCTGG AGGGTGAGGA TATCTATGGG 
AACATCGGAA ACACAGTTCC TAGAATGCCG CATCACCAGG ACAATGGTTA CGGATATGAC 
CAATACGATC TCTATGCCAA TCGAGCGTGC ATCGATCTCG AAAGAAGGCA AGCAGAGGCA 
GCTGCAGCTT CCGGTGGCAG AAATGGGAGG AGAATTGTGA GGCGGCACAG CACCACCACT 
GCGGATCGAG GAGGAAATCC CGGACCGCCC AGGAGGCCTA TGATCAGTCC TGGTTATGAG 
CAGGATCCGC AAGAGATCTA CCAGACCCGC AACGGAGCCT ACATGCTGCC CGATCAGCGA 
AGGGCGCCCA GTTCGGAGGT GATGACCCGC AGAAGATTCT ATCCGGGGGC TGCCAATGAG 
CAAACTGAGG AGGAGCCCCT CTACCAATCC CGTCGCGAAA TGCAGCGTGA GATGCAGAGG 
AATCATCTGT ACCAATCGAA ACGGGAAATG CAGGAGAGGA TTAGCCAGGG CAAGAGGGAT 
ATGGAGCGAG AGTTTAGCCC CCAGGGCAGC AGCAGCCAGT CGGAGACCTC CAATCCGGAG 
GTAATATACC AGAGTCGCCG GGAGGCCAAG GAAAGTGCTC TGAAAACGAG GGCACAACTG 
AGGGATCAGA TCTATCAGAC TAGACGGGAA GCCCTGGACA GCATGGCGGA ACCGATCTAT 
GTGTCCAAAA GAGATATGGG CAGACCGGCT CCTATCTATG AGACTCGGGA AGAGAGTATC 
CTCCAGTCCC GAGAAAATGA AACCGACGAA AAGAAGGAAA AGGAGTGCAA GACCGAGCAG 
ATTCAGGTGG AGATTAATGC TCAACCGGAG GAACAGGTGG AAGATTCGAC CTTAAGTCGA 
TCCGACTTGC AGAAATCTTC GGACACGGTG ATAGAAAATC CAGCGAGAGC TCCTCTTGTG 
CAGGATGAGG TAGATGACGA CGAAGAAGAG CAGGCTCAGG CTCGGGATGA TCCCGCTGAA 
AGCTCTGCGG ATCAGACAGA GCTGCCAACG GCCATTGAAA ACAATCTACA CAACGAGGGA 
ATGGTGGAGC AAACACAGAA TGAGTCGGAT GACGTATTCG AAGCAGCCGA TAAGGTTTCT 
CCTCTAGCCG CTCCTACAGG TACTGTTCAA CCGCCGACCC CGCGATCCAG TAGAGCTCCC 
TTCCACATTT CGAATATCCT AAAGCGAACA GCACCTCCTC CGAGTTCCCC AATGGGCGAC 
AGCTGCACCT CCATCGAAAC CCAGTACACA TCCCAGGCCA GCCTGCCAGT GGGCCCACCA 
AATGCCACCA GTACGCCGTT CAGTAGCAGC ATGTCATTGC CCATCGCCGG ACCCGTAAAC 
AATCAACCGC CGGCCAGCGG ACCCTTTCCC ACTCTGCCCC GCGAACCCAG CACCTCGCGC 
GGATTCTTTG ACTCCAATGG TGGCACTCTG GCAGATAAGC TGTGGCACGT CTCACTGCAA 
ATCCCGCCGG GAGCCATTCC AGCCGGAGTA CGGCAAGAGA TCTACTTCAC CGTGAGCGAT 
CCGCGAATGG GACAAGCCGT CGGCGGTCCT CCGCTGGACA TGGAAAATGG TGAGACAATG 
CTGTCACCGC TGGTGATGTG CGGGCCGCAG GGCCTTGAGT TCCTGGTACC GGTTACACTC 
AACATACCGC ACTGCGCCGG ACGAACTGCA TCACTGGGAC TGGCCCTCAA GGCGACCGAC 
AGCGAGAAGA ACCTGCACAC CGAGTGGGAC AACATCGACC TGCCCAGCAA TGCCGCCGCC 
CACACGGTCT CCGTCAAGGT GGATCACTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_026981913.1 ) cDNA ORF clone, Drosophila erecta -> XP_026837714.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X4, mRNA.

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CloneID	ODi216954
Clone ID Related Accession (Same CDS sequence)	XM_026981913.1
Accession Version	XM_026981913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATAG TCAGCGGATC GGCATCGGGC AACCAGCTCG TCCTGCCAAA CAATCATTTT 
CGCCATGAAT CATTTCAGCA ACAGGGTGAT CGAACCACTT GGGAGTACCA CACGGTGGCA 
GTGACTCGGG TGCCGGGCTA TGGATTCGGC ATCGCCGTTT CCGGTGGACG TGATAATCCG 
CACTTCGCCA ACGGCGATCC CTCGATAGCA GTTAGCGATG TGCTGAAAGG TGGCCCCGCC 
GAGGATCGAT TGCAAGTCAA CGATCGGATA ATCTCGGTGA ACGGCGTCTC CCTGGAGAAC 
GTGGAATATG CCACCGCGGT GCAGGTGTTG CGCGATAGTG GCAACACGGT CCAATTGGTG 
GTCAAGCGTC GCGTGCCCCT CAATCCCATC AATAATGGAG GTGCAGTGCA GCATCAGCAC 
TCGCACAGTC TCAGCTCGGT TGGTCTATTG GCCAATGGAA GTGGAGGAGT TGCACCAACG 
CCCTTGACGA GCCTGAGTCA ACCCAACTCC CTGAACTCTT CGCTGGTGCA GAATGCCAGC 
AGTGGTCAGC CCATCAAGGT GACCCTCACG AAAGGCGGCA AGAAAGATGA CTATGGCGTG 
GTCCTTGGCT GCCGGCTGTT TGTTAAGGAG ATCTCCTCCA AAGCTCGCGA GCAACTTAAT 
GCCAATGGCT ACAGTTTGCA AGAGGGTGAC ATTATCACCC GCATCCACAA CACCAATTGC 
GGGGATACGA TGAGTCTCAA GGAGGCGAAA AAGATCATCG ATGGTTGCAA GGAGCGCTTG 
AACCTAGTGG TTCTACGGGA CATCACCAAC CAAGCAGCTG TTAGCCAACT GAATCTGAAC 
AATTCAGCGA GCCACCAGGC TTCGGCAAAT ATATACGGCA CTCATCAGCC TCAGGTATCC 
GGATGTAGCA GCAGTAACAA CAATCTTGAA GATCCATATC TGCCGGGCGG AGCCAGTTAC 
TCCTCGCAGA ATCTGTACGT GCAACCACCC ACTCGCACCT CCAATGGTCC CAATCTCAAT 
GGCAATGGGC TGAACGACGA AAAGAGTAAT CTCACACCGC GAGGTCGCTC CCGGGGGCCC 
ATTATGGATG GGGTATCTCT GCAGCAGCTG GACAGGCCCG TAACACCGAC GCGTGGCAGG 
AGTGCGGCCA TCGACGAGCC GCCACGTCCA CCGCCGCCAC GAGGATCGAG CGGGGGAGCG 
GCCCAGGAGG ATTTCTACAG CTCTCGCAGG CAATTGTACG AGGAGCGCCA GAGTGCCGAG 
CCGAGATTCA TTTCCTTCCA GAAGGAGGGA AGCGTGGGCA TCCGCCTAAC CGGCGGCAAT 
GAAGCCGGTA TTTTTGTGAC TGCCGTACAA CCGGGATCTC CAGCCTCGCT GCAGGGTCTA 
ATGCCAGGCG ATAAGATCCT GAAGGTCAAC GACATGGACA TGAACGGAGT GACGCGCGAG 
GAGGCTGTTC TCTTTCTGTT GAGCCTGCAG GATCGCATCG ATCTGATTGT GCAGTACTGC 
AAGGAGGAGT ACGACGAGGT GGTGACCAAC CAACGTGGCG ACTCCTTCCA CATCAAGACA 
CATTTCCACT GCGATAATCC CTCCAAAGGC GAGATGGCCT TCAAGGCGGG CGATGTGTTC 
CGGGTGATCG ATACCCTGCA CAATGGTGTA GTCGGTTCCT GGCAGGTGCT GAAGATCGGA 
CGTGGTCACC AGGAGATGCA GCGCGGCGTT ATACCGAACA AGTCGCGGGC CGAAGAACTG 
GCCACTGCCC AGTTCAATGC CACCAAGAAG GAGATGAACG CCAACGAGTC GAGGGGCAAC 
TTCTTCCGGC GACGACGCAG CTCGACGCAC CGACGCTCGA AGAGTCTGTC CCGCGAGAAC 
TGGGACGACG TGGTCTTCTC CGACAGCATT TCCAAATTCC CCGCCTACGA GCGAGTCGTT 
CTCCGCCATC CTGGCTTTGT TCGACCCGTA GTGCTCTTCG GACCCGTATC CGATCTGGCC 
CGCGAGCGCT TGGCCAAGGA TTTCCCCGAC AAGTTTTCGA CTCCACTGCA GGATGATGAC 
AAGTCAGCTG CAACCAGTGG AAAGTGCCGC ATCGTAAGGC TCTCGAACAT ACGCGATGTG 
ATGGATCGTG GCAAGCACGC CCTGCTGGAC ATAACGCCCA ATGCAGTGGA TCGTCTCAAC 
TACGCCCAGT TCTATCCGGT GGTGATCTTC CTGAAGACAG ACAGCAAGCA CGTGATCAAG 
CAGCTGCGCC ACGGACTACC CAAGGCGGCC CACAAGAGCT CCAAGAAGCT GCTGGAGCAG 
ACCCAGAAAC TGGAGCGCGT CTGGTCCCAC ATCTTTAGCA CCCAGATCGC GTTGAGCGAC 
GAGGAGTCCT GGTACCGAAA GTTGCGCGAC TCGATCGATC TGCAGCAGAG CGGCGCCGTG 
TGGATGTCCG AGTCCAAGCC AGTAGAATCC CTCTCCGACG ATTTCCTATT CCCCATGACC 
ACATCCCGAC TCTCGTATGC ATCAAGTCCC GAATCCGATT TGGAACTGAG TCCCGGCCCA 
TCCGCATCAT TGTCGCTTGG CAATTTGCCG CAGTTGGTTA AAGCGAGCTC AGATCCATCG 
ATTGCCACTA ATCAGGATAA CTTGGATAGG GATAGAGACA TAATTGGTGA AGGACTACCA 
CCTCCATATA CGGTACCCTA CGACCATGGT GTCCCCGCGA ATCCCAATCG CCGCCAGACC 
ATGGACTCCA GCAAGTACAG CATCTACGGC ACCAACGTGC CGCCGCAGCC GCAGCAGCCC 
GGCGCAGGAG GAGATCCGGC TGCAGTGAGG CCACAGTCTC TGTACGGGAT TAATGCACCC 
GATCTGCCGC CCCGCATCGA TCGTCAGTCC AAGCCGGGTG ATATGCCACT GAACACCTCG 
GGCTCGTCGT CGCGAAATGG CACCCTGGGT CGCAGTGCCC AGGAGCGTCT GTTTGGCAAG 
GCTGTGGTGC AGGACGATGT CCAGGCGGAA TACATAACCC GAAATGCTCT GGTCGGCTCC 
GGGGCGGCTG AAACCCTGGA TCGCCAGCAG CAGCAGCAAA CCCACGCGTC CTTGGAGCGG 
CAGGCGCGTT TGAATGCGCA GCTGAAGGCC AACGGAGGAG GCGCCGGAGG AGGTGCATCC 
ACCTACGACA GTGTGTCCAG CTACGACTCG TACAACAACA CACAAATGGC CATGCAAAAC 
CTGGGCCGAC TGGGTCCCAA TGCACCCGAC GATCTCAAAT CAGTGCCAAA TGCAAGTGGT 
CGCCCCTTGC CTCCGACGGG TCAGTCGCTC GACTACGGAC GCACTCCGCA CGATCACCGC 
AGCTTCGGAG GAGCCAACGA TCTGAACCGA CAGAGCAGTC CGGGCAGACC GCACTACCAT 
GACATGAATG CGTCCCGTAA TATTGATCCA CGCAATGGCA CTCCACAGCG TCCTTCGAAT 
CTGGGCCTAG AAAGCAGTCC ACGCAAGCCT TTGGTGGAGA CGAAGACGGA TTACGGCAAA 
TACAGTCGAA ACAACTCCGT ATCGCAAGCG GATTACACCA AGCTACCGAA GACGGCGCCA 
CATGGAGTGG TACCGCCGCC GAATGTGAGC AACGGCCAAA GCCAGATGAA TGGCAGTGGA 
ACGCCCAGCA GCAATGGCAG TGGACCCTTC AAGCCGGTGC CGCCACCCAA ACCCAAGAAC 
TATAGGCCAC CGGTGCAAAG TGGTGGCAGC AGTGGCAGCG GTGGCACTAC TCCGTGGGAA 
AATGGGGACT CTGGCTCGCC ACGCTCTCCA AATGGCTTCT ATTATCCACC AACGCCTTCG 
CATCATCACT ACGGCCAGCA GGCGACTCCC GGTTCACCCA GCAACGGCCA CATGCAGCCA 
CCTCCGCCGC AGCAGCAGCA GCAGCAGCCC ACCTACGGCG GAAGTAATGG CAACTACGGA 
CAGGCGCCAC CACCACAACC CTATCCGCAG GCCAATGGCT ACAACGGGAA TGGCCATCAC 
TACAACGGAG GCAGCGGCAC GGGACCCTAC ATTGCACCAC ATCGCGGCAT GCCGCCGCCA 
ATAGGAAACC TGCCGCCCCA CACGCCCGAA CGTCATGCCC TGGACTTGGC CGGAAGTCGG 
GAGCAGCGTG GCTCGGCCTT CGAGCTGTAT CGTAAGCCGC AAATCGGAGC AACCGCTGGG 
CACCATCACA ACATGAGCGA AATGGAGCCG TACGACGAGC GATACGATGA TTACTATAAC 
ATGCCACCCC CTGCCGCCCA TCCATCCCAA GGGCACCATA TGCAGCGCTC CCGATCAGCT 
CCTCGTTATC CCCAGGAGCG ACCTCCTCAC GCACAAGATC CGAATTACTA CGGCCACTAT 
GGAACTTCTA GGGGACATAG TCAGCCGCGC CAGCAGTATC CTCAGCATCA CCAGCAACAG 
TATTACGATG AGCATGGCAT GGAGATGGGT CCACCGCCAC TGCCCCCACA TAAGAAGAAG 
AAATCGGTGC TGAAGAGCCC ACTGGTGGCC CTCAAGAATG CGCTGCTTAA GAGCACGCGG 
CCCCTGAGGA GAATGAACAG CATGGTGGAG CCGGAGAGGA AACCGAAGGG ACTCCGACGC 
CAGCAATCGA TGCTGGAGAG GGGTGTGCAA AGACCCTACT ATCCGGATGA GTATCCAACC 
TATCCAGCTG GCTTTGAGGA GCGTGCCCAT GGCGGACAAG GCATGATGCA GCCTCATCCG 
CAAGATGCCT ACTATCAAAG GGGTGGTCAC TACACGTCGC AGCAGCAGGA GATGATGAAC 
AGCACCTACC AGAATCTGGA GGGTGAGGAT ATCTATGGGA ACATCGGAAA CACAGTTCCT 
AGAATGCCGC ATCACCAGGA CAATGGTTAC GGATATGACC AATACGATCT CTATGCCAAT 
CGAGCGTGCA TCGATCTCGA AAGAAGGCAA GCAGAGGCAG CTGCAGCTTC CGGTGGCAGA 
AATGGGAGGA GAATTGTGAG GCGGCACAGC ACCACCACTG CGGATCGAGG AGGAAATCCC 
GGACCGCCCA GGAGGCCTAT GATCAGTCCT GGTTATGAGC AGGATCCGCA AGAGATCTAC 
CAGACCCGCA ACGGAGCCTA CATGCTGCCC GATCAGCGAA GGGCGCCCAG TTCGGAGGTG 
ATGACCCGCA GAAGATTCTA TCCGGGGGCT GCCAATGAGC AAACTGAGGA GGAGCCCCTC 
TACCAATCCC GTCGCGAAAT GCAGCGTGAG ATGCAGAGGA ATCATCTGTA CCAATCGAAA 
CGGGAAATGC AGGAGAGGAT TAGCCAGGGC AAGAGGGATA TGGAGCGAGA GTTTAGCCCC 
CAGGGCAGCA GCAGCCAGTC GGAGACCTCC AATCCGGAGG TAATATACCA GAGTCGCCGG 
GAGGCCAAGG AAAGTGCTCT GAAAACGAGG GCACAACTGA GGGATCAGAT CTATCAGACT 
AGACGGGAAG CCCTGGACAG CATGGCGGAA CCGATCTATG TGTCCAAAAG AGATATGGGC 
AGACCGGCTC CTATCTATGA GACTCGGGAA GAGAGTATCC TCCAGTCCCG AGAAAATGAA 
ACCGACGAAA AGAAGGAAAA GGAGTGCAAG ACCGAGCAGA TTCAGGTGGA GATTAATGCT 
CAACCGGAGG AACAGGTGGA AGATTCGACC TTAAGTCGAT CCGACTTGCA GAAATCTTCG 
GACACGGTGA TAGAAAATCC AGCGAGAGCT CCTCTTGTGC AGGATGAGGT AGATGACGAC 
GAAGAAGAGC AGGCTCAGGC TCGGGATGAT CCCGCTGAAA GCTCTGCGGA TCAGACAGAG 
CTGCCAACGG CCATTGAAAA CAATCTACAC AACGAGGGAA TGGTGGAGCA AACACAGAAT 
GAGTCGGATG ACGTATTCGA AGCAGCCGAT AAGGTTTCTC CTCTAGCCGC TCCTACAGGT 
ACTGTTCAAC CGCCGACCCC GCGATCCAGT AGAGCTCCCT TCCACATTTC GAATATCCTA 
AAGCGAACAG CACCTCCTCC GAGTTCCCCA ATGGGCGACA GCTGCACCTC CATCGAAACC 
CAGTACACAT CCCAGGCCAG CCTGCCAGTG GGCCCACCAA ATGCCACCAG TACGCCGTTC 
AGTAGCAGCA TGTCATTGCC CATCGCCGGA CCCGTAAACA ATCAACCGCC GGCCAGCGGA 
CCCTTTCCCA CTCTGCCCCG CGAACCCAGC ACCTCGCGCG GATTCTTTGA CTCCAATGGT 
GGCACTCTGG CAGATAAGCT GTGGCACGTC TCACTGCAAA TCCCGCCGGG AGCCATTCCA 
GCCGGAGTAC GGCAAGAGAT CTACTTCACC GTGAGCGATC CGCGAATGGG ACAAGCCGTC 
GGCGGTCCTC CGCTGGACAT GGAAAATGGT GAGACAATGC TGTCACCGCT GGTGATGTGC 
GGGCCGCAGG GCCTTGAGTT CCTGGTACCG GTTACACTCA ACATACCGCA CTGCGCCGGA 
CGAACTGCAT CACTGGGACT GGCCCTCAAG GCGACCGACA GCGAGAAGAA CCTGCACACC 
GAGTGGGACA ACATCGACCT GCCCAGCAAT GCCGCCGCCC ACACGGTCTC CGTCAAGGTG 
GATCACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026837714.1
CDS	586..7137
Translation	MDIVSGSASGNQLVLPNNHFRHESFQQQGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_026981913.1
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026981913.1

ORF Insert Sequence:

ATGGATATAG TCAGCGGATC GGCATCGGGC AACCAGCTCG TCCTGCCAAA CAATCATTTT 
CGCCATGAAT CATTTCAGCA ACAGGGTGAT CGAACCACTT GGGAGTACCA CACGGTGGCA 
GTGACTCGGG TGCCGGGCTA TGGATTCGGC ATCGCCGTTT CCGGTGGACG TGATAATCCG 
CACTTCGCCA ACGGCGATCC CTCGATAGCA GTTAGCGATG TGCTGAAAGG TGGCCCCGCC 
GAGGATCGAT TGCAAGTCAA CGATCGGATA ATCTCGGTGA ACGGCGTCTC CCTGGAGAAC 
GTGGAATATG CCACCGCGGT GCAGGTGTTG CGCGATAGTG GCAACACGGT CCAATTGGTG 
GTCAAGCGTC GCGTGCCCCT CAATCCCATC AATAATGGAG GTGCAGTGCA GCATCAGCAC 
TCGCACAGTC TCAGCTCGGT TGGTCTATTG GCCAATGGAA GTGGAGGAGT TGCACCAACG 
CCCTTGACGA GCCTGAGTCA ACCCAACTCC CTGAACTCTT CGCTGGTGCA GAATGCCAGC 
AGTGGTCAGC CCATCAAGGT GACCCTCACG AAAGGCGGCA AGAAAGATGA CTATGGCGTG 
GTCCTTGGCT GCCGGCTGTT TGTTAAGGAG ATCTCCTCCA AAGCTCGCGA GCAACTTAAT 
GCCAATGGCT ACAGTTTGCA AGAGGGTGAC ATTATCACCC GCATCCACAA CACCAATTGC 
GGGGATACGA TGAGTCTCAA GGAGGCGAAA AAGATCATCG ATGGTTGCAA GGAGCGCTTG 
AACCTAGTGG TTCTACGGGA CATCACCAAC CAAGCAGCTG TTAGCCAACT GAATCTGAAC 
AATTCAGCGA GCCACCAGGC TTCGGCAAAT ATATACGGCA CTCATCAGCC TCAGGTATCC 
GGATGTAGCA GCAGTAACAA CAATCTTGAA GATCCATATC TGCCGGGCGG AGCCAGTTAC 
TCCTCGCAGA ATCTGTACGT GCAACCACCC ACTCGCACCT CCAATGGTCC CAATCTCAAT 
GGCAATGGGC TGAACGACGA AAAGAGTAAT CTCACACCGC GAGGTCGCTC CCGGGGGCCC 
ATTATGGATG GGGTATCTCT GCAGCAGCTG GACAGGCCCG TAACACCGAC GCGTGGCAGG 
AGTGCGGCCA TCGACGAGCC GCCACGTCCA CCGCCGCCAC GAGGATCGAG CGGGGGAGCG 
GCCCAGGAGG ATTTCTACAG CTCTCGCAGG CAATTGTACG AGGAGCGCCA GAGTGCCGAG 
CCGAGATTCA TTTCCTTCCA GAAGGAGGGA AGCGTGGGCA TCCGCCTAAC CGGCGGCAAT 
GAAGCCGGTA TTTTTGTGAC TGCCGTACAA CCGGGATCTC CAGCCTCGCT GCAGGGTCTA 
ATGCCAGGCG ATAAGATCCT GAAGGTCAAC GACATGGACA TGAACGGAGT GACGCGCGAG 
GAGGCTGTTC TCTTTCTGTT GAGCCTGCAG GATCGCATCG ATCTGATTGT GCAGTACTGC 
AAGGAGGAGT ACGACGAGGT GGTGACCAAC CAACGTGGCG ACTCCTTCCA CATCAAGACA 
CATTTCCACT GCGATAATCC CTCCAAAGGC GAGATGGCCT TCAAGGCGGG CGATGTGTTC 
CGGGTGATCG ATACCCTGCA CAATGGTGTA GTCGGTTCCT GGCAGGTGCT GAAGATCGGA 
CGTGGTCACC AGGAGATGCA GCGCGGCGTT ATACCGAACA AGTCGCGGGC CGAAGAACTG 
GCCACTGCCC AGTTCAATGC CACCAAGAAG GAGATGAACG CCAACGAGTC GAGGGGCAAC 
TTCTTCCGGC GACGACGCAG CTCGACGCAC CGACGCTCGA AGAGTCTGTC CCGCGAGAAC 
TGGGACGACG TGGTCTTCTC CGACAGCATT TCCAAATTCC CCGCCTACGA GCGAGTCGTT 
CTCCGCCATC CTGGCTTTGT TCGACCCGTA GTGCTCTTCG GACCCGTATC CGATCTGGCC 
CGCGAGCGCT TGGCCAAGGA TTTCCCCGAC AAGTTTTCGA CTCCACTGCA GGATGATGAC 
AAGTCAGCTG CAACCAGTGG AAAGTGCCGC ATCGTAAGGC TCTCGAACAT ACGCGATGTG 
ATGGATCGTG GCAAGCACGC CCTGCTGGAC ATAACGCCCA ATGCAGTGGA TCGTCTCAAC 
TACGCCCAGT TCTATCCGGT GGTGATCTTC CTGAAGACAG ACAGCAAGCA CGTGATCAAG 
CAGCTGCGCC ACGGACTACC CAAGGCGGCC CACAAGAGCT CCAAGAAGCT GCTGGAGCAG 
ACCCAGAAAC TGGAGCGCGT CTGGTCCCAC ATCTTTAGCA CCCAGATCGC GTTGAGCGAC 
GAGGAGTCCT GGTACCGAAA GTTGCGCGAC TCGATCGATC TGCAGCAGAG CGGCGCCGTG 
TGGATGTCCG AGTCCAAGCC AGTAGAATCC CTCTCCGACG ATTTCCTATT CCCCATGACC 
ACATCCCGAC TCTCGTATGC ATCAAGTCCC GAATCCGATT TGGAACTGAG TCCCGGCCCA 
TCCGCATCAT TGTCGCTTGG CAATTTGCCG CAGTTGGTTA AAGCGAGCTC AGATCCATCG 
ATTGCCACTA ATCAGGATAA CTTGGATAGG GATAGAGACA TAATTGGTGA AGGACTACCA 
CCTCCATATA CGGTACCCTA CGACCATGGT GTCCCCGCGA ATCCCAATCG CCGCCAGACC 
ATGGACTCCA GCAAGTACAG CATCTACGGC ACCAACGTGC CGCCGCAGCC GCAGCAGCCC 
GGCGCAGGAG GAGATCCGGC TGCAGTGAGG CCACAGTCTC TGTACGGGAT TAATGCACCC 
GATCTGCCGC CCCGCATCGA TCGTCAGTCC AAGCCGGGTG ATATGCCACT GAACACCTCG 
GGCTCGTCGT CGCGAAATGG CACCCTGGGT CGCAGTGCCC AGGAGCGTCT GTTTGGCAAG 
GCTGTGGTGC AGGACGATGT CCAGGCGGAA TACATAACCC GAAATGCTCT GGTCGGCTCC 
GGGGCGGCTG AAACCCTGGA TCGCCAGCAG CAGCAGCAAA CCCACGCGTC CTTGGAGCGG 
CAGGCGCGTT TGAATGCGCA GCTGAAGGCC AACGGAGGAG GCGCCGGAGG AGGTGCATCC 
ACCTACGACA GTGTGTCCAG CTACGACTCG TACAACAACA CACAAATGGC CATGCAAAAC 
CTGGGCCGAC TGGGTCCCAA TGCACCCGAC GATCTCAAAT CAGTGCCAAA TGCAAGTGGT 
CGCCCCTTGC CTCCGACGGG TCAGTCGCTC GACTACGGAC GCACTCCGCA CGATCACCGC 
AGCTTCGGAG GAGCCAACGA TCTGAACCGA CAGAGCAGTC CGGGCAGACC GCACTACCAT 
GACATGAATG CGTCCCGTAA TATTGATCCA CGCAATGGCA CTCCACAGCG TCCTTCGAAT 
CTGGGCCTAG AAAGCAGTCC ACGCAAGCCT TTGGTGGAGA CGAAGACGGA TTACGGCAAA 
TACAGTCGAA ACAACTCCGT ATCGCAAGCG GATTACACCA AGCTACCGAA GACGGCGCCA 
CATGGAGTGG TACCGCCGCC GAATGTGAGC AACGGCCAAA GCCAGATGAA TGGCAGTGGA 
ACGCCCAGCA GCAATGGCAG TGGACCCTTC AAGCCGGTGC CGCCACCCAA ACCCAAGAAC 
TATAGGCCAC CGGTGCAAAG TGGTGGCAGC AGTGGCAGCG GTGGCACTAC TCCGTGGGAA 
AATGGGGACT CTGGCTCGCC ACGCTCTCCA AATGGCTTCT ATTATCCACC AACGCCTTCG 
CATCATCACT ACGGCCAGCA GGCGACTCCC GGTTCACCCA GCAACGGCCA CATGCAGCCA 
CCTCCGCCGC AGCAGCAGCA GCAGCAGCCC ACCTACGGCG GAAGTAATGG CAACTACGGA 
CAGGCGCCAC CACCACAACC CTATCCGCAG GCCAATGGCT ACAACGGGAA TGGCCATCAC 
TACAACGGAG GCAGCGGCAC GGGACCCTAC ATTGCACCAC ATCGCGGCAT GCCGCCGCCA 
ATAGGAAACC TGCCGCCCCA CACGCCCGAA CGTCATGCCC TGGACTTGGC CGGAAGTCGG 
GAGCAGCGTG GCTCGGCCTT CGAGCTGTAT CGTAAGCCGC AAATCGGAGC AACCGCTGGG 
CACCATCACA ACATGAGCGA AATGGAGCCG TACGACGAGC GATACGATGA TTACTATAAC 
ATGCCACCCC CTGCCGCCCA TCCATCCCAA GGGCACCATA TGCAGCGCTC CCGATCAGCT 
CCTCGTTATC CCCAGGAGCG ACCTCCTCAC GCACAAGATC CGAATTACTA CGGCCACTAT 
GGAACTTCTA GGGGACATAG TCAGCCGCGC CAGCAGTATC CTCAGCATCA CCAGCAACAG 
TATTACGATG AGCATGGCAT GGAGATGGGT CCACCGCCAC TGCCCCCACA TAAGAAGAAG 
AAATCGGTGC TGAAGAGCCC ACTGGTGGCC CTCAAGAATG CGCTGCTTAA GAGCACGCGG 
CCCCTGAGGA GAATGAACAG CATGGTGGAG CCGGAGAGGA AACCGAAGGG ACTCCGACGC 
CAGCAATCGA TGCTGGAGAG GGGTGTGCAA AGACCCTACT ATCCGGATGA GTATCCAACC 
TATCCAGCTG GCTTTGAGGA GCGTGCCCAT GGCGGACAAG GCATGATGCA GCCTCATCCG 
CAAGATGCCT ACTATCAAAG GGGTGGTCAC TACACGTCGC AGCAGCAGGA GATGATGAAC 
AGCACCTACC AGAATCTGGA GGGTGAGGAT ATCTATGGGA ACATCGGAAA CACAGTTCCT 
AGAATGCCGC ATCACCAGGA CAATGGTTAC GGATATGACC AATACGATCT CTATGCCAAT 
CGAGCGTGCA TCGATCTCGA AAGAAGGCAA GCAGAGGCAG CTGCAGCTTC CGGTGGCAGA 
AATGGGAGGA GAATTGTGAG GCGGCACAGC ACCACCACTG CGGATCGAGG AGGAAATCCC 
GGACCGCCCA GGAGGCCTAT GATCAGTCCT GGTTATGAGC AGGATCCGCA AGAGATCTAC 
CAGACCCGCA ACGGAGCCTA CATGCTGCCC GATCAGCGAA GGGCGCCCAG TTCGGAGGTG 
ATGACCCGCA GAAGATTCTA TCCGGGGGCT GCCAATGAGC AAACTGAGGA GGAGCCCCTC 
TACCAATCCC GTCGCGAAAT GCAGCGTGAG ATGCAGAGGA ATCATCTGTA CCAATCGAAA 
CGGGAAATGC AGGAGAGGAT TAGCCAGGGC AAGAGGGATA TGGAGCGAGA GTTTAGCCCC 
CAGGGCAGCA GCAGCCAGTC GGAGACCTCC AATCCGGAGG TAATATACCA GAGTCGCCGG 
GAGGCCAAGG AAAGTGCTCT GAAAACGAGG GCACAACTGA GGGATCAGAT CTATCAGACT 
AGACGGGAAG CCCTGGACAG CATGGCGGAA CCGATCTATG TGTCCAAAAG AGATATGGGC 
AGACCGGCTC CTATCTATGA GACTCGGGAA GAGAGTATCC TCCAGTCCCG AGAAAATGAA 
ACCGACGAAA AGAAGGAAAA GGAGTGCAAG ACCGAGCAGA TTCAGGTGGA GATTAATGCT 
CAACCGGAGG AACAGGTGGA AGATTCGACC TTAAGTCGAT CCGACTTGCA GAAATCTTCG 
GACACGGTGA TAGAAAATCC AGCGAGAGCT CCTCTTGTGC AGGATGAGGT AGATGACGAC 
GAAGAAGAGC AGGCTCAGGC TCGGGATGAT CCCGCTGAAA GCTCTGCGGA TCAGACAGAG 
CTGCCAACGG CCATTGAAAA CAATCTACAC AACGAGGGAA TGGTGGAGCA AACACAGAAT 
GAGTCGGATG ACGTATTCGA AGCAGCCGAT AAGGTTTCTC CTCTAGCCGC TCCTACAGGT 
ACTGTTCAAC CGCCGACCCC GCGATCCAGT AGAGCTCCCT TCCACATTTC GAATATCCTA 
AAGCGAACAG CACCTCCTCC GAGTTCCCCA ATGGGCGACA GCTGCACCTC CATCGAAACC 
CAGTACACAT CCCAGGCCAG CCTGCCAGTG GGCCCACCAA ATGCCACCAG TACGCCGTTC 
AGTAGCAGCA TGTCATTGCC CATCGCCGGA CCCGTAAACA ATCAACCGCC GGCCAGCGGA 
CCCTTTCCCA CTCTGCCCCG CGAACCCAGC ACCTCGCGCG GATTCTTTGA CTCCAATGGT 
GGCACTCTGG CAGATAAGCT GTGGCACGTC TCACTGCAAA TCCCGCCGGG AGCCATTCCA 
GCCGGAGTAC GGCAAGAGAT CTACTTCACC GTGAGCGATC CGCGAATGGG ACAAGCCGTC 
GGCGGTCCTC CGCTGGACAT GGAAAATGGT GAGACAATGC TGTCACCGCT GGTGATGTGC 
GGGCCGCAGG GCCTTGAGTT CCTGGTACCG GTTACACTCA ACATACCGCA CTGCGCCGGA 
CGAACTGCAT CACTGGGACT GGCCCTCAAG GCGACCGACA GCGAGAAGAA CCTGCACACC 
GAGTGGGACA ACATCGACCT GCCCAGCAAT GCCGCCGCCC ACACGGTCTC CGTCAAGGTG 
GATCACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_026981923.1 ) cDNA ORF clone, Drosophila erecta -> XP_026837724.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X6, mRNA.

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CloneID	ODi216955
Clone ID Related Accession (Same CDS sequence)	XM_026981923.1 , XM_026981920.1
Accession Version	XM_026981923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCAGCTCGA CGCACCGACG CTCGAAGAGT 
CTGTCCCGCG AGAACTGGGA CGACGTGGTC TTCTCCGACA GCATTTCCAA ATTCCCCGCC 
TACGAGCGAG TCGTTCTCCG CCATCCTGGC TTTGTTCGAC CCGTAGTGCT CTTCGGACCC 
GTATCCGATC TGGCCCGCGA GCGCTTGGCC AAGGATTTCC CCGACAAGTT TTCGACTCCA 
CTGCAGGATG ATGACAAGTC AGCTGCAACC AGTGGAAAGT GCCGCATCGT AAGGCTCTCG 
AACATACGCG ATGTGATGGA TCGTGGCAAG CACGCCCTGC TGGACATAAC GCCCAATGCA 
GTGGATCGTC TCAACTACGC CCAGTTCTAT CCGGTGGTGA TCTTCCTGAA GACAGACAGC 
AAGCACGTGA TCAAGCAGCT GCGCCACGGA CTACCCAAGG CGGCCCACAA GAGCTCCAAG 
AAGCTGCTGG AGCAGACCCA GAAACTGGAG CGCGTCTGGT CCCACATCTT TAGCACCCAG 
ATCGCGTTGA GCGACGAGGA GTCCTGGTAC CGAAAGTTGC GCGACTCGAT CGATCTGCAG 
CAGAGCGGCG CCGTGTGGAT GTCCGAGTCC AAGCCAGTAG AATCCCTCTC CGACGATTTC 
CTATTCCCCA TGACCACATC CCGACTCTCG TATGCATCAA GTCCCGAATC CGATTTGGAA 
CTGAGTCCCG GCCCATCCGC ATCATTGTCG CTTGGCAATT TGCCGCAGTT GGTTAAAGCG 
AGCTCAGATC CATCGATTGC CACTAATCAG GATAACTTGG ATAGGGATAG AGACATAATT 
GGTGAAGGAC TACCACCTCC ATATACGGTA CCCTACGACC ATGGTGTCCC CGCGAATCCC 
AATCGCCGCC AGACCATGGA CTCCAGCAAG TACAGCATCT ACGGCACCAA CGTGCCGCCG 
CAGCCGCAGC AGCCCGGCGC AGGAGGAGAT CCGGCTGCAG TGAGGCCACA GTCTCTGTAC 
GGGATTAATG CACCCGATCT GCCGCCCCGC ATCGATCGTC AGTCCAAGCC GGGTGATATG 
CCACTGAACA CCTCGGGCTC GTCGTCGCGA AATGGCACCC TGGGTCGCAG TGCCCAGGAG 
CGTCTGTTTG GCAAGGCTGT GGTGCAGGAC GATGTCCAGG CGGAATACAT AACCCGAAAT 
GCTCTGGTCG GCTCCGGGGC GGCTGAAACC CTGGATCGCC AGCAGCAGCA GCAAACCCAC 
GCGTCCTTGG AGCGGCAGGC GCGTTTGAAT GCGCAGCTGA AGGCCAACGG AGGAGGCGCC 
GGAGGAGGTG CATCCACCTA CGACAGTGTG TCCAGCTACG ACTCGTACAA CAACACACAA 
ATGGCCATGC AAAACCTGGG CCGACTGGGT CCCAATGCAC CCGACGATCT CAAATCAGTG 
CCAAATGCAA GTGGTCGCCC CTTGCCTCCG ACGGGTCAGT CGCTCGACTA CGGACGCACT 
CCGCACGATC ACCGCAGCTT CGGAGGAGCC AACGATCTGA ACCGACAGAG CAGTCCGGGC 
AGACCGCACT ACCATGACAT GAATGCGTCC CGTAATATTG ATCCACGCAA TGGCACTCCA 
CAGCGTCCTT CGAATCTGGG CCTAGAAAGC AGTCCACGCA AGCCTTTGGT GGAGACGAAG 
ACGGATTACG GCAAATACAG TCGAAACAAC TCCGTATCGC AAGCGGATTA CACCAAGCTA 
CCGAAGACGG CGCCACATGG AGTGGTACCG CCGCCGAATG TGAGCAACGG CCAAAGCCAG 
ATGAATGGCA GTGGAACGCC CAGCAGCAAT GGCAGTGGAC CCTTCAAGCC GGTGCCGCCA 
CCCAAACCCA AGAACTATAG GCCACCGGTG CAAAGTGGTG GCAGCAGTGG CAGCGGTGGC 
ACTACTCCGT GGGAAAATGG GGACTCTGGC TCGCCACGCT CTCCAAATGG CTTCTATTAT 
CCACCAACGC CTTCGCATCA TCACTACGGC CAGCAGGCGA CTCCCGGTTC ACCCAGCAAC 
GGCCACATGC AGCCACCTCC GCCGCAGCAG CAGCAGCAGC AGCCCACCTA CGGCGGAAGT 
AATGGCAACT ACGGACAGGC GCCACCACCA CAACCCTATC CGCAGGCCAA TGGCTACAAC 
GGGAATGGCC ATCACTACAA CGGAGGCAGC GGCACGGGAC CCTACATTGC ACCACATCGC 
GGCATGCCGC CGCCAATAGG AAACCTGCCG CCCCACACGC CCGAACGTCA TGCCCTGGAC 
TTGGCCGGAA GTCGGGAGCA GCGTGGCTCG GCCTTCGAGC TGTATCGTAA GCCGCAAATC 
GGAGCAACCG CTGGGCACCA TCACAACATG AGCGAAATGG AGCCGTACGA CGAGCGATAC 
GATGATTACT ATAACATGCC ACCCCCTGCC GCCCATCCAT CCCAAGGGCA CCATATGCAG 
CGCTCCCGAT CAGCTCCTCG TTATCCCCAG GAGCGACCTC CTCACGCACA AGATCCGAAT 
TACTACGGCC ACTATGGAAC TTCTAGGGGA CATAGTCAGC CGCGCCAGCA GTATCCTCAG 
CATCACCAGC AACAGTATTA CGATGAGCAT GGCATGGAGA TGGGTCCACC GCCACTGCCC 
CCACATAAGA AGAAGAAATC GGTGCTGAAG AGCCCACTGG TGGCCCTCAA GAATGCGCTG 
CTTAAGAGCA CGCGGCCCCT GAGGAGAATG AACAGCATGG TGGAGCCGGA GAGGAAACCG 
AAGGGACTCC GACGCCAGCA ATCGATGCTG GAGAGGGGTG TGCAAAGACC CTACTATCCG 
GATGAGTATC CAACCTATCC AGCTGGCTTT GAGGAGCGTG CCCATGGCGG ACAAGGCATG 
ATGCAGCCTC ATCCGCAAGA TGCCTACTAT CAAAGGGGTG GTCACTACAC GTCGCAGCAG 
CAGGAGATGA TGAACAGCAC CTACCAGAAT CTGGAGGGTG AGGATATCTA TGGGAACATC 
GGAAACACAG TTCCTAGAAT GCCGCATCAC CAGGACAATG GTTACGGATA TGACCAATAC 
GATCTCTATG CCAATCGAGC GTGCATCGAT CTCGAAAGAA GGCAAGCAGA GGCAGCTGCA 
GCTTCCGGTG GCAGAAATGG GAGGAGAATT GTGAGGCGGC ACAGCACCAC CACTGCGGAT 
CGAGGAGGAA ATCCCGGACC GCCCAGGAGG CCTATGATCA GTCCTGGTTA TGAGCAGGAT 
CCGCAAGAGA TCTACCAGAC CCGCAACGGA GCCTACATGC TGCCCGATCA GCGAAGGGCG 
CCCAGTTCGG AGGTGATGAC CCGCAGAAGA TTCTATCCGG GGGCTGCCAA TGAGCAAACT 
GAGGAGGAGC CCCTCTACCA ATCCCGTCGC GAAATGCAGC GTGAGATGCA GAGGAATCAT 
CTGTACCAAT CGAAACGGGA AATGCAGGAG AGGATTAGCC AGGGCAAGAG GGATATGGAG 
CGAGAGTTTA GCCCCCAGGG CAGCAGCAGC CAGTCGGAGA CCTCCAATCC GGAGGTAATA 
TACCAGAGTC GCCGGGAGGC CAAGGAAAGT GCTCTGAAAA CGAGGGCACA ACTGAGGGAT 
CAGATCTATC AGACTAGACG GGAAGCCCTG GACAGCATGG CGGAACCGAT CTATGTGTCC 
AAAAGAGATA TGGGCAGACC GGCTCCTATC TATGAGACTC GGGAAGAGAG TATCCTCCAG 
TCCCGAGAAA ATGAAACCGA CGAAAAGAAG GAAAAGGAGT GCAAGACCGA GCAGATTCAG 
GTGGAGATTA ATGCTCAACC GGAGGAACAG GTGGAAGATT CGACCTTAAG TCGATCCGAC 
TTGCAGAAAT CTTCGGACAC GGTGATAGAA AATCCAGCGA GAGCTCCTCT TGTGCAGGAT 
GAGGTAGATG ACGACGAAGA AGAGCAGGCT CAGGCTCGGG ATGATCCCGC TGAAAGCTCT 
GCGGATCAGA CAGAGCTGCC AACGGCCATT GAAAACAATC TACACAACGA GGGAATGGTG 
GAGCAAACAC AGAATGAGTC GGATGACGTA TTCGAAGCAG CCGATAAGGT TTCTCCTCTA 
GCCGCTCCTA CAGGTACTGT TCAACCGCCG ACCCCGCGAT CCAGTAGAGC TCCCTTCCAC 
ATTTCGAATA TCCTAAAGCG AACAGCACCT CCTCCGAGTT CCCCAATGGG CGACAGCTGC 
ACCTCCATCG AAACCCAGTA CACATCCCAG GCCAGCCTGC CAGTGGGCCC ACCAAATGCC 
ACCAGTACGC CGTTCAGTAG CAGCATGTCA TTGCCCATCG CCGGACCCGT AAACAATCAA 
CCGCCGGCCA GCGGACCCTT TCCCACTCTG CCCCGCGAAC CCAGCACCTC GCGCGGATTC 
TTTGACTCCA ATGGTGGCAC TCTGGCAGAT AAGCTGTGGC ACGTCTCACT GCAAATCCCG 
CCGGGAGCCA TTCCAGCCGG AGTACGGCAA GAGATCTACT TCACCGTGAG CGATCCGCGA 
ATGGGACAAG CCGTCGGCGG TCCTCCGCTG GACATGGAAA ATGGTGAGAC AATGCTGTCA 
CCGCTGGTGA TGTGCGGGCC GCAGGGCCTT GAGTTCCTGG TACCGGTTAC ACTCAACATA 
CCGCACTGCG CCGGACGAAC TGCATCACTG GGACTGGCCC TCAAGGCGAC CGACAGCGAG 
AAGAACCTGC ACACCGAGTG GGACAACATC GACCTGCCCA GCAATGCCGC CGCCCACACG 
GTCTCCGTCA AGGTGGATCA CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026837724.1
CDS	334..6840
Translation	MKFPDYVPNNMLVGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_026981923.1
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026981923.1

ORF Insert Sequence:

ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCAGCTCGA CGCACCGACG CTCGAAGAGT 
CTGTCCCGCG AGAACTGGGA CGACGTGGTC TTCTCCGACA GCATTTCCAA ATTCCCCGCC 
TACGAGCGAG TCGTTCTCCG CCATCCTGGC TTTGTTCGAC CCGTAGTGCT CTTCGGACCC 
GTATCCGATC TGGCCCGCGA GCGCTTGGCC AAGGATTTCC CCGACAAGTT TTCGACTCCA 
CTGCAGGATG ATGACAAGTC AGCTGCAACC AGTGGAAAGT GCCGCATCGT AAGGCTCTCG 
AACATACGCG ATGTGATGGA TCGTGGCAAG CACGCCCTGC TGGACATAAC GCCCAATGCA 
GTGGATCGTC TCAACTACGC CCAGTTCTAT CCGGTGGTGA TCTTCCTGAA GACAGACAGC 
AAGCACGTGA TCAAGCAGCT GCGCCACGGA CTACCCAAGG CGGCCCACAA GAGCTCCAAG 
AAGCTGCTGG AGCAGACCCA GAAACTGGAG CGCGTCTGGT CCCACATCTT TAGCACCCAG 
ATCGCGTTGA GCGACGAGGA GTCCTGGTAC CGAAAGTTGC GCGACTCGAT CGATCTGCAG 
CAGAGCGGCG CCGTGTGGAT GTCCGAGTCC AAGCCAGTAG AATCCCTCTC CGACGATTTC 
CTATTCCCCA TGACCACATC CCGACTCTCG TATGCATCAA GTCCCGAATC CGATTTGGAA 
CTGAGTCCCG GCCCATCCGC ATCATTGTCG CTTGGCAATT TGCCGCAGTT GGTTAAAGCG 
AGCTCAGATC CATCGATTGC CACTAATCAG GATAACTTGG ATAGGGATAG AGACATAATT 
GGTGAAGGAC TACCACCTCC ATATACGGTA CCCTACGACC ATGGTGTCCC CGCGAATCCC 
AATCGCCGCC AGACCATGGA CTCCAGCAAG TACAGCATCT ACGGCACCAA CGTGCCGCCG 
CAGCCGCAGC AGCCCGGCGC AGGAGGAGAT CCGGCTGCAG TGAGGCCACA GTCTCTGTAC 
GGGATTAATG CACCCGATCT GCCGCCCCGC ATCGATCGTC AGTCCAAGCC GGGTGATATG 
CCACTGAACA CCTCGGGCTC GTCGTCGCGA AATGGCACCC TGGGTCGCAG TGCCCAGGAG 
CGTCTGTTTG GCAAGGCTGT GGTGCAGGAC GATGTCCAGG CGGAATACAT AACCCGAAAT 
GCTCTGGTCG GCTCCGGGGC GGCTGAAACC CTGGATCGCC AGCAGCAGCA GCAAACCCAC 
GCGTCCTTGG AGCGGCAGGC GCGTTTGAAT GCGCAGCTGA AGGCCAACGG AGGAGGCGCC 
GGAGGAGGTG CATCCACCTA CGACAGTGTG TCCAGCTACG ACTCGTACAA CAACACACAA 
ATGGCCATGC AAAACCTGGG CCGACTGGGT CCCAATGCAC CCGACGATCT CAAATCAGTG 
CCAAATGCAA GTGGTCGCCC CTTGCCTCCG ACGGGTCAGT CGCTCGACTA CGGACGCACT 
CCGCACGATC ACCGCAGCTT CGGAGGAGCC AACGATCTGA ACCGACAGAG CAGTCCGGGC 
AGACCGCACT ACCATGACAT GAATGCGTCC CGTAATATTG ATCCACGCAA TGGCACTCCA 
CAGCGTCCTT CGAATCTGGG CCTAGAAAGC AGTCCACGCA AGCCTTTGGT GGAGACGAAG 
ACGGATTACG GCAAATACAG TCGAAACAAC TCCGTATCGC AAGCGGATTA CACCAAGCTA 
CCGAAGACGG CGCCACATGG AGTGGTACCG CCGCCGAATG TGAGCAACGG CCAAAGCCAG 
ATGAATGGCA GTGGAACGCC CAGCAGCAAT GGCAGTGGAC CCTTCAAGCC GGTGCCGCCA 
CCCAAACCCA AGAACTATAG GCCACCGGTG CAAAGTGGTG GCAGCAGTGG CAGCGGTGGC 
ACTACTCCGT GGGAAAATGG GGACTCTGGC TCGCCACGCT CTCCAAATGG CTTCTATTAT 
CCACCAACGC CTTCGCATCA TCACTACGGC CAGCAGGCGA CTCCCGGTTC ACCCAGCAAC 
GGCCACATGC AGCCACCTCC GCCGCAGCAG CAGCAGCAGC AGCCCACCTA CGGCGGAAGT 
AATGGCAACT ACGGACAGGC GCCACCACCA CAACCCTATC CGCAGGCCAA TGGCTACAAC 
GGGAATGGCC ATCACTACAA CGGAGGCAGC GGCACGGGAC CCTACATTGC ACCACATCGC 
GGCATGCCGC CGCCAATAGG AAACCTGCCG CCCCACACGC CCGAACGTCA TGCCCTGGAC 
TTGGCCGGAA GTCGGGAGCA GCGTGGCTCG GCCTTCGAGC TGTATCGTAA GCCGCAAATC 
GGAGCAACCG CTGGGCACCA TCACAACATG AGCGAAATGG AGCCGTACGA CGAGCGATAC 
GATGATTACT ATAACATGCC ACCCCCTGCC GCCCATCCAT CCCAAGGGCA CCATATGCAG 
CGCTCCCGAT CAGCTCCTCG TTATCCCCAG GAGCGACCTC CTCACGCACA AGATCCGAAT 
TACTACGGCC ACTATGGAAC TTCTAGGGGA CATAGTCAGC CGCGCCAGCA GTATCCTCAG 
CATCACCAGC AACAGTATTA CGATGAGCAT GGCATGGAGA TGGGTCCACC GCCACTGCCC 
CCACATAAGA AGAAGAAATC GGTGCTGAAG AGCCCACTGG TGGCCCTCAA GAATGCGCTG 
CTTAAGAGCA CGCGGCCCCT GAGGAGAATG AACAGCATGG TGGAGCCGGA GAGGAAACCG 
AAGGGACTCC GACGCCAGCA ATCGATGCTG GAGAGGGGTG TGCAAAGACC CTACTATCCG 
GATGAGTATC CAACCTATCC AGCTGGCTTT GAGGAGCGTG CCCATGGCGG ACAAGGCATG 
ATGCAGCCTC ATCCGCAAGA TGCCTACTAT CAAAGGGGTG GTCACTACAC GTCGCAGCAG 
CAGGAGATGA TGAACAGCAC CTACCAGAAT CTGGAGGGTG AGGATATCTA TGGGAACATC 
GGAAACACAG TTCCTAGAAT GCCGCATCAC CAGGACAATG GTTACGGATA TGACCAATAC 
GATCTCTATG CCAATCGAGC GTGCATCGAT CTCGAAAGAA GGCAAGCAGA GGCAGCTGCA 
GCTTCCGGTG GCAGAAATGG GAGGAGAATT GTGAGGCGGC ACAGCACCAC CACTGCGGAT 
CGAGGAGGAA ATCCCGGACC GCCCAGGAGG CCTATGATCA GTCCTGGTTA TGAGCAGGAT 
CCGCAAGAGA TCTACCAGAC CCGCAACGGA GCCTACATGC TGCCCGATCA GCGAAGGGCG 
CCCAGTTCGG AGGTGATGAC CCGCAGAAGA TTCTATCCGG GGGCTGCCAA TGAGCAAACT 
GAGGAGGAGC CCCTCTACCA ATCCCGTCGC GAAATGCAGC GTGAGATGCA GAGGAATCAT 
CTGTACCAAT CGAAACGGGA AATGCAGGAG AGGATTAGCC AGGGCAAGAG GGATATGGAG 
CGAGAGTTTA GCCCCCAGGG CAGCAGCAGC CAGTCGGAGA CCTCCAATCC GGAGGTAATA 
TACCAGAGTC GCCGGGAGGC CAAGGAAAGT GCTCTGAAAA CGAGGGCACA ACTGAGGGAT 
CAGATCTATC AGACTAGACG GGAAGCCCTG GACAGCATGG CGGAACCGAT CTATGTGTCC 
AAAAGAGATA TGGGCAGACC GGCTCCTATC TATGAGACTC GGGAAGAGAG TATCCTCCAG 
TCCCGAGAAA ATGAAACCGA CGAAAAGAAG GAAAAGGAGT GCAAGACCGA GCAGATTCAG 
GTGGAGATTA ATGCTCAACC GGAGGAACAG GTGGAAGATT CGACCTTAAG TCGATCCGAC 
TTGCAGAAAT CTTCGGACAC GGTGATAGAA AATCCAGCGA GAGCTCCTCT TGTGCAGGAT 
GAGGTAGATG ACGACGAAGA AGAGCAGGCT CAGGCTCGGG ATGATCCCGC TGAAAGCTCT 
GCGGATCAGA CAGAGCTGCC AACGGCCATT GAAAACAATC TACACAACGA GGGAATGGTG 
GAGCAAACAC AGAATGAGTC GGATGACGTA TTCGAAGCAG CCGATAAGGT TTCTCCTCTA 
GCCGCTCCTA CAGGTACTGT TCAACCGCCG ACCCCGCGAT CCAGTAGAGC TCCCTTCCAC 
ATTTCGAATA TCCTAAAGCG AACAGCACCT CCTCCGAGTT CCCCAATGGG CGACAGCTGC 
ACCTCCATCG AAACCCAGTA CACATCCCAG GCCAGCCTGC CAGTGGGCCC ACCAAATGCC 
ACCAGTACGC CGTTCAGTAG CAGCATGTCA TTGCCCATCG CCGGACCCGT AAACAATCAA 
CCGCCGGCCA GCGGACCCTT TCCCACTCTG CCCCGCGAAC CCAGCACCTC GCGCGGATTC 
TTTGACTCCA ATGGTGGCAC TCTGGCAGAT AAGCTGTGGC ACGTCTCACT GCAAATCCCG 
CCGGGAGCCA TTCCAGCCGG AGTACGGCAA GAGATCTACT TCACCGTGAG CGATCCGCGA 
ATGGGACAAG CCGTCGGCGG TCCTCCGCTG GACATGGAAA ATGGTGAGAC AATGCTGTCA 
CCGCTGGTGA TGTGCGGGCC GCAGGGCCTT GAGTTCCTGG TACCGGTTAC ACTCAACATA 
CCGCACTGCG CCGGACGAAC TGCATCACTG GGACTGGCCC TCAAGGCGAC CGACAGCGAG 
AAGAACCTGC ACACCGAGTG GGACAACATC GACCTGCCCA GCAATGCCGC CGCCCACACG 
GTCTCCGTCA AGGTGGATCA CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_026981920.1 ) cDNA ORF clone, Drosophila erecta -> XP_026837721.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X5, mRNA.

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CloneID	ODi216955
Clone ID Related Accession (Same CDS sequence)	XM_026981923.1 , XM_026981920.1
Accession Version	XM_026981920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCAGCTCGA CGCACCGACG CTCGAAGAGT 
CTGTCCCGCG AGAACTGGGA CGACGTGGTC TTCTCCGACA GCATTTCCAA ATTCCCCGCC 
TACGAGCGAG TCGTTCTCCG CCATCCTGGC TTTGTTCGAC CCGTAGTGCT CTTCGGACCC 
GTATCCGATC TGGCCCGCGA GCGCTTGGCC AAGGATTTCC CCGACAAGTT TTCGACTCCA 
CTGCAGGATG ATGACAAGTC AGCTGCAACC AGTGGAAAGT GCCGCATCGT AAGGCTCTCG 
AACATACGCG ATGTGATGGA TCGTGGCAAG CACGCCCTGC TGGACATAAC GCCCAATGCA 
GTGGATCGTC TCAACTACGC CCAGTTCTAT CCGGTGGTGA TCTTCCTGAA GACAGACAGC 
AAGCACGTGA TCAAGCAGCT GCGCCACGGA CTACCCAAGG CGGCCCACAA GAGCTCCAAG 
AAGCTGCTGG AGCAGACCCA GAAACTGGAG CGCGTCTGGT CCCACATCTT TAGCACCCAG 
ATCGCGTTGA GCGACGAGGA GTCCTGGTAC CGAAAGTTGC GCGACTCGAT CGATCTGCAG 
CAGAGCGGCG CCGTGTGGAT GTCCGAGTCC AAGCCAGTAG AATCCCTCTC CGACGATTTC 
CTATTCCCCA TGACCACATC CCGACTCTCG TATGCATCAA GTCCCGAATC CGATTTGGAA 
CTGAGTCCCG GCCCATCCGC ATCATTGTCG CTTGGCAATT TGCCGCAGTT GGTTAAAGCG 
AGCTCAGATC CATCGATTGC CACTAATCAG GATAACTTGG ATAGGGATAG AGACATAATT 
GGTGAAGGAC TACCACCTCC ATATACGGTA CCCTACGACC ATGGTGTCCC CGCGAATCCC 
AATCGCCGCC AGACCATGGA CTCCAGCAAG TACAGCATCT ACGGCACCAA CGTGCCGCCG 
CAGCCGCAGC AGCCCGGCGC AGGAGGAGAT CCGGCTGCAG TGAGGCCACA GTCTCTGTAC 
GGGATTAATG CACCCGATCT GCCGCCCCGC ATCGATCGTC AGTCCAAGCC GGGTGATATG 
CCACTGAACA CCTCGGGCTC GTCGTCGCGA AATGGCACCC TGGGTCGCAG TGCCCAGGAG 
CGTCTGTTTG GCAAGGCTGT GGTGCAGGAC GATGTCCAGG CGGAATACAT AACCCGAAAT 
GCTCTGGTCG GCTCCGGGGC GGCTGAAACC CTGGATCGCC AGCAGCAGCA GCAAACCCAC 
GCGTCCTTGG AGCGGCAGGC GCGTTTGAAT GCGCAGCTGA AGGCCAACGG AGGAGGCGCC 
GGAGGAGGTG CATCCACCTA CGACAGTGTG TCCAGCTACG ACTCGTACAA CAACACACAA 
ATGGCCATGC AAAACCTGGG CCGACTGGGT CCCAATGCAC CCGACGATCT CAAATCAGTG 
CCAAATGCAA GTGGTCGCCC CTTGCCTCCG ACGGGTCAGT CGCTCGACTA CGGACGCACT 
CCGCACGATC ACCGCAGCTT CGGAGGAGCC AACGATCTGA ACCGACAGAG CAGTCCGGGC 
AGACCGCACT ACCATGACAT GAATGCGTCC CGTAATATTG ATCCACGCAA TGGCACTCCA 
CAGCGTCCTT CGAATCTGGG CCTAGAAAGC AGTCCACGCA AGCCTTTGGT GGAGACGAAG 
ACGGATTACG GCAAATACAG TCGAAACAAC TCCGTATCGC AAGCGGATTA CACCAAGCTA 
CCGAAGACGG CGCCACATGG AGTGGTACCG CCGCCGAATG TGAGCAACGG CCAAAGCCAG 
ATGAATGGCA GTGGAACGCC CAGCAGCAAT GGCAGTGGAC CCTTCAAGCC GGTGCCGCCA 
CCCAAACCCA AGAACTATAG GCCACCGGTG CAAAGTGGTG GCAGCAGTGG CAGCGGTGGC 
ACTACTCCGT GGGAAAATGG GGACTCTGGC TCGCCACGCT CTCCAAATGG CTTCTATTAT 
CCACCAACGC CTTCGCATCA TCACTACGGC CAGCAGGCGA CTCCCGGTTC ACCCAGCAAC 
GGCCACATGC AGCCACCTCC GCCGCAGCAG CAGCAGCAGC AGCCCACCTA CGGCGGAAGT 
AATGGCAACT ACGGACAGGC GCCACCACCA CAACCCTATC CGCAGGCCAA TGGCTACAAC 
GGGAATGGCC ATCACTACAA CGGAGGCAGC GGCACGGGAC CCTACATTGC ACCACATCGC 
GGCATGCCGC CGCCAATAGG AAACCTGCCG CCCCACACGC CCGAACGTCA TGCCCTGGAC 
TTGGCCGGAA GTCGGGAGCA GCGTGGCTCG GCCTTCGAGC TGTATCGTAA GCCGCAAATC 
GGAGCAACCG CTGGGCACCA TCACAACATG AGCGAAATGG AGCCGTACGA CGAGCGATAC 
GATGATTACT ATAACATGCC ACCCCCTGCC GCCCATCCAT CCCAAGGGCA CCATATGCAG 
CGCTCCCGAT CAGCTCCTCG TTATCCCCAG GAGCGACCTC CTCACGCACA AGATCCGAAT 
TACTACGGCC ACTATGGAAC TTCTAGGGGA CATAGTCAGC CGCGCCAGCA GTATCCTCAG 
CATCACCAGC AACAGTATTA CGATGAGCAT GGCATGGAGA TGGGTCCACC GCCACTGCCC 
CCACATAAGA AGAAGAAATC GGTGCTGAAG AGCCCACTGG TGGCCCTCAA GAATGCGCTG 
CTTAAGAGCA CGCGGCCCCT GAGGAGAATG AACAGCATGG TGGAGCCGGA GAGGAAACCG 
AAGGGACTCC GACGCCAGCA ATCGATGCTG GAGAGGGGTG TGCAAAGACC CTACTATCCG 
GATGAGTATC CAACCTATCC AGCTGGCTTT GAGGAGCGTG CCCATGGCGG ACAAGGCATG 
ATGCAGCCTC ATCCGCAAGA TGCCTACTAT CAAAGGGGTG GTCACTACAC GTCGCAGCAG 
CAGGAGATGA TGAACAGCAC CTACCAGAAT CTGGAGGGTG AGGATATCTA TGGGAACATC 
GGAAACACAG TTCCTAGAAT GCCGCATCAC CAGGACAATG GTTACGGATA TGACCAATAC 
GATCTCTATG CCAATCGAGC GTGCATCGAT CTCGAAAGAA GGCAAGCAGA GGCAGCTGCA 
GCTTCCGGTG GCAGAAATGG GAGGAGAATT GTGAGGCGGC ACAGCACCAC CACTGCGGAT 
CGAGGAGGAA ATCCCGGACC GCCCAGGAGG CCTATGATCA GTCCTGGTTA TGAGCAGGAT 
CCGCAAGAGA TCTACCAGAC CCGCAACGGA GCCTACATGC TGCCCGATCA GCGAAGGGCG 
CCCAGTTCGG AGGTGATGAC CCGCAGAAGA TTCTATCCGG GGGCTGCCAA TGAGCAAACT 
GAGGAGGAGC CCCTCTACCA ATCCCGTCGC GAAATGCAGC GTGAGATGCA GAGGAATCAT 
CTGTACCAAT CGAAACGGGA AATGCAGGAG AGGATTAGCC AGGGCAAGAG GGATATGGAG 
CGAGAGTTTA GCCCCCAGGG CAGCAGCAGC CAGTCGGAGA CCTCCAATCC GGAGGTAATA 
TACCAGAGTC GCCGGGAGGC CAAGGAAAGT GCTCTGAAAA CGAGGGCACA ACTGAGGGAT 
CAGATCTATC AGACTAGACG GGAAGCCCTG GACAGCATGG CGGAACCGAT CTATGTGTCC 
AAAAGAGATA TGGGCAGACC GGCTCCTATC TATGAGACTC GGGAAGAGAG TATCCTCCAG 
TCCCGAGAAA ATGAAACCGA CGAAAAGAAG GAAAAGGAGT GCAAGACCGA GCAGATTCAG 
GTGGAGATTA ATGCTCAACC GGAGGAACAG GTGGAAGATT CGACCTTAAG TCGATCCGAC 
TTGCAGAAAT CTTCGGACAC GGTGATAGAA AATCCAGCGA GAGCTCCTCT TGTGCAGGAT 
GAGGTAGATG ACGACGAAGA AGAGCAGGCT CAGGCTCGGG ATGATCCCGC TGAAAGCTCT 
GCGGATCAGA CAGAGCTGCC AACGGCCATT GAAAACAATC TACACAACGA GGGAATGGTG 
GAGCAAACAC AGAATGAGTC GGATGACGTA TTCGAAGCAG CCGATAAGGT TTCTCCTCTA 
GCCGCTCCTA CAGGTACTGT TCAACCGCCG ACCCCGCGAT CCAGTAGAGC TCCCTTCCAC 
ATTTCGAATA TCCTAAAGCG AACAGCACCT CCTCCGAGTT CCCCAATGGG CGACAGCTGC 
ACCTCCATCG AAACCCAGTA CACATCCCAG GCCAGCCTGC CAGTGGGCCC ACCAAATGCC 
ACCAGTACGC CGTTCAGTAG CAGCATGTCA TTGCCCATCG CCGGACCCGT AAACAATCAA 
CCGCCGGCCA GCGGACCCTT TCCCACTCTG CCCCGCGAAC CCAGCACCTC GCGCGGATTC 
TTTGACTCCA ATGGTGGCAC TCTGGCAGAT AAGCTGTGGC ACGTCTCACT GCAAATCCCG 
CCGGGAGCCA TTCCAGCCGG AGTACGGCAA GAGATCTACT TCACCGTGAG CGATCCGCGA 
ATGGGACAAG CCGTCGGCGG TCCTCCGCTG GACATGGAAA ATGGTGAGAC AATGCTGTCA 
CCGCTGGTGA TGTGCGGGCC GCAGGGCCTT GAGTTCCTGG TACCGGTTAC ACTCAACATA 
CCGCACTGCG CCGGACGAAC TGCATCACTG GGACTGGCCC TCAAGGCGAC CGACAGCGAG 
AAGAACCTGC ACACCGAGTG GGACAACATC GACCTGCCCA GCAATGCCGC CGCCCACACG 
GTCTCCGTCA AGGTGGATCA CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026837721.1
CDS	480..6986
Translation	MKFPDYVPNNMLVGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_026981920.1
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026981920.1

ORF Insert Sequence:

ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCAGCTCGA CGCACCGACG CTCGAAGAGT 
CTGTCCCGCG AGAACTGGGA CGACGTGGTC TTCTCCGACA GCATTTCCAA ATTCCCCGCC 
TACGAGCGAG TCGTTCTCCG CCATCCTGGC TTTGTTCGAC CCGTAGTGCT CTTCGGACCC 
GTATCCGATC TGGCCCGCGA GCGCTTGGCC AAGGATTTCC CCGACAAGTT TTCGACTCCA 
CTGCAGGATG ATGACAAGTC AGCTGCAACC AGTGGAAAGT GCCGCATCGT AAGGCTCTCG 
AACATACGCG ATGTGATGGA TCGTGGCAAG CACGCCCTGC TGGACATAAC GCCCAATGCA 
GTGGATCGTC TCAACTACGC CCAGTTCTAT CCGGTGGTGA TCTTCCTGAA GACAGACAGC 
AAGCACGTGA TCAAGCAGCT GCGCCACGGA CTACCCAAGG CGGCCCACAA GAGCTCCAAG 
AAGCTGCTGG AGCAGACCCA GAAACTGGAG CGCGTCTGGT CCCACATCTT TAGCACCCAG 
ATCGCGTTGA GCGACGAGGA GTCCTGGTAC CGAAAGTTGC GCGACTCGAT CGATCTGCAG 
CAGAGCGGCG CCGTGTGGAT GTCCGAGTCC AAGCCAGTAG AATCCCTCTC CGACGATTTC 
CTATTCCCCA TGACCACATC CCGACTCTCG TATGCATCAA GTCCCGAATC CGATTTGGAA 
CTGAGTCCCG GCCCATCCGC ATCATTGTCG CTTGGCAATT TGCCGCAGTT GGTTAAAGCG 
AGCTCAGATC CATCGATTGC CACTAATCAG GATAACTTGG ATAGGGATAG AGACATAATT 
GGTGAAGGAC TACCACCTCC ATATACGGTA CCCTACGACC ATGGTGTCCC CGCGAATCCC 
AATCGCCGCC AGACCATGGA CTCCAGCAAG TACAGCATCT ACGGCACCAA CGTGCCGCCG 
CAGCCGCAGC AGCCCGGCGC AGGAGGAGAT CCGGCTGCAG TGAGGCCACA GTCTCTGTAC 
GGGATTAATG CACCCGATCT GCCGCCCCGC ATCGATCGTC AGTCCAAGCC GGGTGATATG 
CCACTGAACA CCTCGGGCTC GTCGTCGCGA AATGGCACCC TGGGTCGCAG TGCCCAGGAG 
CGTCTGTTTG GCAAGGCTGT GGTGCAGGAC GATGTCCAGG CGGAATACAT AACCCGAAAT 
GCTCTGGTCG GCTCCGGGGC GGCTGAAACC CTGGATCGCC AGCAGCAGCA GCAAACCCAC 
GCGTCCTTGG AGCGGCAGGC GCGTTTGAAT GCGCAGCTGA AGGCCAACGG AGGAGGCGCC 
GGAGGAGGTG CATCCACCTA CGACAGTGTG TCCAGCTACG ACTCGTACAA CAACACACAA 
ATGGCCATGC AAAACCTGGG CCGACTGGGT CCCAATGCAC CCGACGATCT CAAATCAGTG 
CCAAATGCAA GTGGTCGCCC CTTGCCTCCG ACGGGTCAGT CGCTCGACTA CGGACGCACT 
CCGCACGATC ACCGCAGCTT CGGAGGAGCC AACGATCTGA ACCGACAGAG CAGTCCGGGC 
AGACCGCACT ACCATGACAT GAATGCGTCC CGTAATATTG ATCCACGCAA TGGCACTCCA 
CAGCGTCCTT CGAATCTGGG CCTAGAAAGC AGTCCACGCA AGCCTTTGGT GGAGACGAAG 
ACGGATTACG GCAAATACAG TCGAAACAAC TCCGTATCGC AAGCGGATTA CACCAAGCTA 
CCGAAGACGG CGCCACATGG AGTGGTACCG CCGCCGAATG TGAGCAACGG CCAAAGCCAG 
ATGAATGGCA GTGGAACGCC CAGCAGCAAT GGCAGTGGAC CCTTCAAGCC GGTGCCGCCA 
CCCAAACCCA AGAACTATAG GCCACCGGTG CAAAGTGGTG GCAGCAGTGG CAGCGGTGGC 
ACTACTCCGT GGGAAAATGG GGACTCTGGC TCGCCACGCT CTCCAAATGG CTTCTATTAT 
CCACCAACGC CTTCGCATCA TCACTACGGC CAGCAGGCGA CTCCCGGTTC ACCCAGCAAC 
GGCCACATGC AGCCACCTCC GCCGCAGCAG CAGCAGCAGC AGCCCACCTA CGGCGGAAGT 
AATGGCAACT ACGGACAGGC GCCACCACCA CAACCCTATC CGCAGGCCAA TGGCTACAAC 
GGGAATGGCC ATCACTACAA CGGAGGCAGC GGCACGGGAC CCTACATTGC ACCACATCGC 
GGCATGCCGC CGCCAATAGG AAACCTGCCG CCCCACACGC CCGAACGTCA TGCCCTGGAC 
TTGGCCGGAA GTCGGGAGCA GCGTGGCTCG GCCTTCGAGC TGTATCGTAA GCCGCAAATC 
GGAGCAACCG CTGGGCACCA TCACAACATG AGCGAAATGG AGCCGTACGA CGAGCGATAC 
GATGATTACT ATAACATGCC ACCCCCTGCC GCCCATCCAT CCCAAGGGCA CCATATGCAG 
CGCTCCCGAT CAGCTCCTCG TTATCCCCAG GAGCGACCTC CTCACGCACA AGATCCGAAT 
TACTACGGCC ACTATGGAAC TTCTAGGGGA CATAGTCAGC CGCGCCAGCA GTATCCTCAG 
CATCACCAGC AACAGTATTA CGATGAGCAT GGCATGGAGA TGGGTCCACC GCCACTGCCC 
CCACATAAGA AGAAGAAATC GGTGCTGAAG AGCCCACTGG TGGCCCTCAA GAATGCGCTG 
CTTAAGAGCA CGCGGCCCCT GAGGAGAATG AACAGCATGG TGGAGCCGGA GAGGAAACCG 
AAGGGACTCC GACGCCAGCA ATCGATGCTG GAGAGGGGTG TGCAAAGACC CTACTATCCG 
GATGAGTATC CAACCTATCC AGCTGGCTTT GAGGAGCGTG CCCATGGCGG ACAAGGCATG 
ATGCAGCCTC ATCCGCAAGA TGCCTACTAT CAAAGGGGTG GTCACTACAC GTCGCAGCAG 
CAGGAGATGA TGAACAGCAC CTACCAGAAT CTGGAGGGTG AGGATATCTA TGGGAACATC 
GGAAACACAG TTCCTAGAAT GCCGCATCAC CAGGACAATG GTTACGGATA TGACCAATAC 
GATCTCTATG CCAATCGAGC GTGCATCGAT CTCGAAAGAA GGCAAGCAGA GGCAGCTGCA 
GCTTCCGGTG GCAGAAATGG GAGGAGAATT GTGAGGCGGC ACAGCACCAC CACTGCGGAT 
CGAGGAGGAA ATCCCGGACC GCCCAGGAGG CCTATGATCA GTCCTGGTTA TGAGCAGGAT 
CCGCAAGAGA TCTACCAGAC CCGCAACGGA GCCTACATGC TGCCCGATCA GCGAAGGGCG 
CCCAGTTCGG AGGTGATGAC CCGCAGAAGA TTCTATCCGG GGGCTGCCAA TGAGCAAACT 
GAGGAGGAGC CCCTCTACCA ATCCCGTCGC GAAATGCAGC GTGAGATGCA GAGGAATCAT 
CTGTACCAAT CGAAACGGGA AATGCAGGAG AGGATTAGCC AGGGCAAGAG GGATATGGAG 
CGAGAGTTTA GCCCCCAGGG CAGCAGCAGC CAGTCGGAGA CCTCCAATCC GGAGGTAATA 
TACCAGAGTC GCCGGGAGGC CAAGGAAAGT GCTCTGAAAA CGAGGGCACA ACTGAGGGAT 
CAGATCTATC AGACTAGACG GGAAGCCCTG GACAGCATGG CGGAACCGAT CTATGTGTCC 
AAAAGAGATA TGGGCAGACC GGCTCCTATC TATGAGACTC GGGAAGAGAG TATCCTCCAG 
TCCCGAGAAA ATGAAACCGA CGAAAAGAAG GAAAAGGAGT GCAAGACCGA GCAGATTCAG 
GTGGAGATTA ATGCTCAACC GGAGGAACAG GTGGAAGATT CGACCTTAAG TCGATCCGAC 
TTGCAGAAAT CTTCGGACAC GGTGATAGAA AATCCAGCGA GAGCTCCTCT TGTGCAGGAT 
GAGGTAGATG ACGACGAAGA AGAGCAGGCT CAGGCTCGGG ATGATCCCGC TGAAAGCTCT 
GCGGATCAGA CAGAGCTGCC AACGGCCATT GAAAACAATC TACACAACGA GGGAATGGTG 
GAGCAAACAC AGAATGAGTC GGATGACGTA TTCGAAGCAG CCGATAAGGT TTCTCCTCTA 
GCCGCTCCTA CAGGTACTGT TCAACCGCCG ACCCCGCGAT CCAGTAGAGC TCCCTTCCAC 
ATTTCGAATA TCCTAAAGCG AACAGCACCT CCTCCGAGTT CCCCAATGGG CGACAGCTGC 
ACCTCCATCG AAACCCAGTA CACATCCCAG GCCAGCCTGC CAGTGGGCCC ACCAAATGCC 
ACCAGTACGC CGTTCAGTAG CAGCATGTCA TTGCCCATCG CCGGACCCGT AAACAATCAA 
CCGCCGGCCA GCGGACCCTT TCCCACTCTG CCCCGCGAAC CCAGCACCTC GCGCGGATTC 
TTTGACTCCA ATGGTGGCAC TCTGGCAGAT AAGCTGTGGC ACGTCTCACT GCAAATCCCG 
CCGGGAGCCA TTCCAGCCGG AGTACGGCAA GAGATCTACT TCACCGTGAG CGATCCGCGA 
ATGGGACAAG CCGTCGGCGG TCCTCCGCTG GACATGGAAA ATGGTGAGAC AATGCTGTCA 
CCGCTGGTGA TGTGCGGGCC GCAGGGCCTT GAGTTCCTGG TACCGGTTAC ACTCAACATA 
CCGCACTGCG CCGGACGAAC TGCATCACTG GGACTGGCCC TCAAGGCGAC CGACAGCGAG 
AAGAACCTGC ACACCGAGTG GGACAACATC GACCTGCCCA GCAATGCCGC CGCCCACACG 
GTCTCCGTCA AGGTGGATCA CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_001980861.2 ) cDNA ORF clone, Drosophila erecta -> XP_001980897.2 Drosophila erecta uncharacterized protein, transcript variant F (Dere\GG17413), mRNA.

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CloneID	ODi33444
Clone ID Related Accession (Same CDS sequence)	XM_001980861.2 , XM_026981926.1
Accession Version	XM_001980861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956552). On Dec 21, 2015 this sequence version replaced XM_001980861.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCTCGACGC ACCGACGCTC GAAGAGTCTG 
TCCCGCGAGA ACTGGGACGA CGTGGTCTTC TCCGACAGCA TTTCCAAATT CCCCGCCTAC 
GAGCGAGTCG TTCTCCGCCA TCCTGGCTTT GTTCGACCCG TAGTGCTCTT CGGACCCGTA 
TCCGATCTGG CCCGCGAGCG CTTGGCCAAG GATTTCCCCG ACAAGTTTTC GACTCCACTG 
CAGGATGATG ACAAGTCAGC TGCAACCAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAC 
ATACGCGATG TGATGGATCG TGGCAAGCAC GCCCTGCTGG ACATAACGCC CAATGCAGTG 
GATCGTCTCA ACTACGCCCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG 
CACGTGATCA AGCAGCTGCG CCACGGACTA CCCAAGGCGG CCCACAAGAG CTCCAAGAAG 
CTGCTGGAGC AGACCCAGAA ACTGGAGCGC GTCTGGTCCC ACATCTTTAG CACCCAGATC 
GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ACTCGATCGA TCTGCAGCAG 
AGCGGCGCCG TGTGGATGTC CGAGTCCAAG CCAGTAGAAT CCCTCTCCGA CGATTTCCTA 
TTCCCCATGA CCACATCCCG ACTCTCGTAT GCATCAAGTC CCGAATCCGA TTTGGAACTG 
AGTCCCGGCC CATCCGCATC ATTGTCGCTT GGCAATTTGC CGCAGTTGGT TAAAGCGAGC 
TCAGATCCAT CGATTGCCAC TAATCAGGAT AACTTGGATA GGGATAGAGA CATAATTGGT 
GAAGGACTAC CACCTCCATA TACGGTACCC TACGACCATG GTGTCCCCGC GAATCCCAAT 
CGCCGCCAGA CCATGGACTC CAGCAAGTAC AGCATCTACG GCACCAACGT GCCGCCGCAG 
CCGCAGCAGC CCGGCGCAGG AGGAGATCCG GCTGCAGTGA GGCCACAGTC TCTGTACGGG 
ATTAATGCAC CCGATCTGCC GCCCCGCATC GATCGTCAGT CCAAGCCGGG TGATATGCCA 
CTGAACACCT CGGGCTCGTC GTCGCGAAAT GGCACCCTGG GTCGCAGTGC CCAGGAGCGT 
CTGTTTGGCA AGGCTGTGGT GCAGGACGAT GTCCAGGCGG AATACATAAC CCGAAATGCT 
CTGGTCGGCT CCGGGGCGGC TGAAACCCTG GATCGCCAGC AGCAGCAGCA AACCCACGCG 
TCCTTGGAGC GGCAGGCGCG TTTGAATGCG CAGCTGAAGG CCAACGGAGG AGGCGCCGGA 
GGAGGTGCAT CCACCTACGA CAGTGTGTCC AGCTACGACT CGTACAACAA CACACAAATG 
GCCATGCAAA ACCTGGGCCG ACTGGGTCCC AATGCACCCG ACGATCTCAA ATCAGTGCCA 
AATGCAAGTG GTCGCCCCTT GCCTCCGACG GGTCAGTCGC TCGACTACGG ACGCACTCCG 
CACGATCACC GCAGCTTCGG AGGAGCCAAC GATCTGAACC GACAGAGCAG TCCGGGCAGA 
CCGCACTACC ATGACATGAA TGCGTCCCGT AATATTGATC CACGCAATGG CACTCCACAG 
CGTCCTTCGA ATCTGGGCCT AGAAAGCAGT CCACGCAAGC CTTTGGTGGA GACGAAGACG 
GATTACGGCA AATACAGTCG AAACAACTCC GTATCGCAAG CGGATTACAC CAAGCTACCG 
AAGACGGCGC CACATGGAGT GGTACCGCCG CCGAATGTGA GCAACGGCCA AAGCCAGATG 
AATGGCAGTG GAACGCCCAG CAGCAATGGC AGTGGACCCT TCAAGCCGGT GCCGCCACCC 
AAACCCAAGA ACTATAGGCC ACCGGTGCAA AGTGGTGGCA GCAGTGGCAG CGGTGGCACT 
ACTCCGTGGG AAAATGGGGA CTCTGGCTCG CCACGCTCTC CAAATGGCTT CTATTATCCA 
CCAACGCCTT CGCATCATCA CTACGGCCAG CAGGCGACTC CCGGTTCACC CAGCAACGGC 
CACATGCAGC CACCTCCGCC GCAGCAGCAG CAGCAGCAGC CCACCTACGG CGGAAGTAAT 
GGCAACTACG GACAGGCGCC ACCACCACAA CCCTATCCGC AGGCCAATGG CTACAACGGG 
AATGGCCATC ACTACAACGG AGGCAGCGGC ACGGGACCCT ACATTGCACC ACATCGCGGC 
ATGCCGCCGC CAATAGGAAA CCTGCCGCCC CACACGCCCG AACGTCATGC CCTGGACTTG 
GCCGGAAGTC GGGAGCAGCG TGGCTCGGCC TTCGAGCTGT ATCGTAAGCC GCAAATCGGA 
GCAACCGCTG GGCACCATCA CAACATGAGC GAAATGGAGC CGTACGACGA GCGATACGAT 
GATTACTATA ACATGCCACC CCCTGCCGCC CATCCATCCC AAGGGCACCA TATGCAGCGC 
TCCCGATCAG CTCCTCGTTA TCCCCAGGAG CGACCTCCTC ACGCACAAGA TCCGAATTAC 
TACGGCCACT ATGGAACTTC TAGGGGACAT AGTCAGCCGC GCCAGCAGTA TCCTCAGCAT 
CACCAGCAAC AGTATTACGA TGAGCATGGC ATGGAGATGG GTCCACCGCC ACTGCCCCCA 
CATAAGAAGA AGAAATCGGT GCTGAAGAGC CCACTGGTGG CCCTCAAGAA TGCGCTGCTT 
AAGAGCACGC GGCCCCTGAG GAGAATGAAC AGCATGGTGG AGCCGGAGAG GAAACCGAAG 
GGACTCCGAC GCCAGCAATC GATGCTGGAG AGGGGTGTGC AAAGACCCTA CTATCCGGAT 
GAGTATCCAA CCTATCCAGC TGGCTTTGAG GAGCGTGCCC ATGGCGGACA AGGCATGATG 
CAGCCTCATC CGCAAGATGC CTACTATCAA AGGGGTGGTC ACTACACGTC GCAGCAGCAG 
GAGATGATGA ACAGCACCTA CCAGAATCTG GAGGGTGAGG ATATCTATGG GAACATCGGA 
AACACAGTTC CTAGAATGCC GCATCACCAG GACAATGGTT ACGGATATGA CCAATACGAT 
CTCTATGCCA ATCGAGCGTG CATCGATCTC GAAAGAAGGC AAGCAGAGGC AGCTGCAGCT 
TCCGGTGGCA GAAATGGGAG GAGAATTGTG AGGCGGCACA GCACCACCAC TGCGGATCGA 
GGAGGAAATC CCGGACCGCC CAGGAGGCCT ATGATCAGTC CTGGTTATGA GCAGGATCCG 
CAAGAGATCT ACCAGACCCG CAACGGAGCC TACATGCTGC CCGATCAGCG AAGGGCGCCC 
AGTTCGGAGG TGATGACCCG CAGAAGATTC TATCCGGGGG CTGCCAATGA GCAAACTGAG 
GAGGAGCCCC TCTACCAATC CCGTCGCGAA ATGCAGCGTG AGATGCAGAG GAATCATCTG 
TACCAATCGA AACGGGAAAT GCAGGAGAGG ATTAGCCAGG GCAAGAGGGA TATGGAGCGA 
GAGTTTAGCC CCCAGGGCAG CAGCAGCCAG TCGGAGACCT CCAATCCGGA GGTAATATAC 
CAGAGTCGCC GGGAGGCCAA GGAAAGTGCT CTGAAAACGA GGGCACAACT GAGGGATCAG 
ATCTATCAGA CTAGACGGGA AGCCCTGGAC AGCATGGCGG AACCGATCTA TGTGTCCAAA 
AGAGATATGG GCAGACCGGC TCCTATCTAT GAGACTCGGG AAGAGAGTAT CCTCCAGTCC 
CGAGAAAATG AAACCGACGA AAAGAAGGAA AAGGAGTGCA AGACCGAGCA GATTCAGGTG 
GAGATTAATG CTCAACCGGA GGAACAGGTG GAAGATTCGA CCTTAAGTCG ATCCGACTTG 
CAGAAATCTT CGGACACGGT GATAGAAAAT CCAGCGAGAG CTCCTCTTGT GCAGGATGAG 
GTAGATGACG ACGAAGAAGA GCAGGCTCAG GCTCGGGATG ATCCCGCTGA AAGCTCTGCG 
GATCAGACAG AGCTGCCAAC GGCCATTGAA AACAATCTAC ACAACGAGGG AATGGTGGAG 
CAAACACAGA ATGAGTCGGA TGACGTATTC GAAGCAGCCG ATAAGGTTTC TCCTCTAGCC 
GCTCCTACAG GTACTGTTCA ACCGCCGACC CCGCGATCCA GTAGAGCTCC CTTCCACATT 
TCGAATATCC TAAAGCGAAC AGCACCTCCT CCGAGTTCCC CAATGGGCGA CAGCTGCACC 
TCCATCGAAA CCCAGTACAC ATCCCAGGCC AGCCTGCCAG TGGGCCCACC AAATGCCACC 
AGTACGCCGT TCAGTAGCAG CATGTCATTG CCCATCGCCG GACCCGTAAA CAATCAACCG 
CCGGCCAGCG GACCCTTTCC CACTCTGCCC CGCGAACCCA GCACCTCGCG CGGATTCTTT 
GACTCCAATG GTGGCACTCT GGCAGATAAG CTGTGGCACG TCTCACTGCA AATCCCGCCG 
GGAGCCATTC CAGCCGGAGT ACGGCAAGAG ATCTACTTCA CCGTGAGCGA TCCGCGAATG 
GGACAAGCCG TCGGCGGTCC TCCGCTGGAC ATGGAAAATG GTGAGACAAT GCTGTCACCG 
CTGGTGATGT GCGGGCCGCA GGGCCTTGAG TTCCTGGTAC CGGTTACACT CAACATACCG 
CACTGCGCCG GACGAACTGC ATCACTGGGA CTGGCCCTCA AGGCGACCGA CAGCGAGAAG 
AACCTGCACA CCGAGTGGGA CAACATCGAC CTGCCCAGCA ATGCCGCCGC CCACACGGTC 
TCCGTCAAGG TGGATCACTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001980897.2
CDS	297..6800
Translation	MKFPDYVPNNMLVGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_001980861.2
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant F (Dere\GG17413), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001980861.2

ORF Insert Sequence:

ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCTCGACGC ACCGACGCTC GAAGAGTCTG 
TCCCGCGAGA ACTGGGACGA CGTGGTCTTC TCCGACAGCA TTTCCAAATT CCCCGCCTAC 
GAGCGAGTCG TTCTCCGCCA TCCTGGCTTT GTTCGACCCG TAGTGCTCTT CGGACCCGTA 
TCCGATCTGG CCCGCGAGCG CTTGGCCAAG GATTTCCCCG ACAAGTTTTC GACTCCACTG 
CAGGATGATG ACAAGTCAGC TGCAACCAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAC 
ATACGCGATG TGATGGATCG TGGCAAGCAC GCCCTGCTGG ACATAACGCC CAATGCAGTG 
GATCGTCTCA ACTACGCCCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG 
CACGTGATCA AGCAGCTGCG CCACGGACTA CCCAAGGCGG CCCACAAGAG CTCCAAGAAG 
CTGCTGGAGC AGACCCAGAA ACTGGAGCGC GTCTGGTCCC ACATCTTTAG CACCCAGATC 
GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ACTCGATCGA TCTGCAGCAG 
AGCGGCGCCG TGTGGATGTC CGAGTCCAAG CCAGTAGAAT CCCTCTCCGA CGATTTCCTA 
TTCCCCATGA CCACATCCCG ACTCTCGTAT GCATCAAGTC CCGAATCCGA TTTGGAACTG 
AGTCCCGGCC CATCCGCATC ATTGTCGCTT GGCAATTTGC CGCAGTTGGT TAAAGCGAGC 
TCAGATCCAT CGATTGCCAC TAATCAGGAT AACTTGGATA GGGATAGAGA CATAATTGGT 
GAAGGACTAC CACCTCCATA TACGGTACCC TACGACCATG GTGTCCCCGC GAATCCCAAT 
CGCCGCCAGA CCATGGACTC CAGCAAGTAC AGCATCTACG GCACCAACGT GCCGCCGCAG 
CCGCAGCAGC CCGGCGCAGG AGGAGATCCG GCTGCAGTGA GGCCACAGTC TCTGTACGGG 
ATTAATGCAC CCGATCTGCC GCCCCGCATC GATCGTCAGT CCAAGCCGGG TGATATGCCA 
CTGAACACCT CGGGCTCGTC GTCGCGAAAT GGCACCCTGG GTCGCAGTGC CCAGGAGCGT 
CTGTTTGGCA AGGCTGTGGT GCAGGACGAT GTCCAGGCGG AATACATAAC CCGAAATGCT 
CTGGTCGGCT CCGGGGCGGC TGAAACCCTG GATCGCCAGC AGCAGCAGCA AACCCACGCG 
TCCTTGGAGC GGCAGGCGCG TTTGAATGCG CAGCTGAAGG CCAACGGAGG AGGCGCCGGA 
GGAGGTGCAT CCACCTACGA CAGTGTGTCC AGCTACGACT CGTACAACAA CACACAAATG 
GCCATGCAAA ACCTGGGCCG ACTGGGTCCC AATGCACCCG ACGATCTCAA ATCAGTGCCA 
AATGCAAGTG GTCGCCCCTT GCCTCCGACG GGTCAGTCGC TCGACTACGG ACGCACTCCG 
CACGATCACC GCAGCTTCGG AGGAGCCAAC GATCTGAACC GACAGAGCAG TCCGGGCAGA 
CCGCACTACC ATGACATGAA TGCGTCCCGT AATATTGATC CACGCAATGG CACTCCACAG 
CGTCCTTCGA ATCTGGGCCT AGAAAGCAGT CCACGCAAGC CTTTGGTGGA GACGAAGACG 
GATTACGGCA AATACAGTCG AAACAACTCC GTATCGCAAG CGGATTACAC CAAGCTACCG 
AAGACGGCGC CACATGGAGT GGTACCGCCG CCGAATGTGA GCAACGGCCA AAGCCAGATG 
AATGGCAGTG GAACGCCCAG CAGCAATGGC AGTGGACCCT TCAAGCCGGT GCCGCCACCC 
AAACCCAAGA ACTATAGGCC ACCGGTGCAA AGTGGTGGCA GCAGTGGCAG CGGTGGCACT 
ACTCCGTGGG AAAATGGGGA CTCTGGCTCG CCACGCTCTC CAAATGGCTT CTATTATCCA 
CCAACGCCTT CGCATCATCA CTACGGCCAG CAGGCGACTC CCGGTTCACC CAGCAACGGC 
CACATGCAGC CACCTCCGCC GCAGCAGCAG CAGCAGCAGC CCACCTACGG CGGAAGTAAT 
GGCAACTACG GACAGGCGCC ACCACCACAA CCCTATCCGC AGGCCAATGG CTACAACGGG 
AATGGCCATC ACTACAACGG AGGCAGCGGC ACGGGACCCT ACATTGCACC ACATCGCGGC 
ATGCCGCCGC CAATAGGAAA CCTGCCGCCC CACACGCCCG AACGTCATGC CCTGGACTTG 
GCCGGAAGTC GGGAGCAGCG TGGCTCGGCC TTCGAGCTGT ATCGTAAGCC GCAAATCGGA 
GCAACCGCTG GGCACCATCA CAACATGAGC GAAATGGAGC CGTACGACGA GCGATACGAT 
GATTACTATA ACATGCCACC CCCTGCCGCC CATCCATCCC AAGGGCACCA TATGCAGCGC 
TCCCGATCAG CTCCTCGTTA TCCCCAGGAG CGACCTCCTC ACGCACAAGA TCCGAATTAC 
TACGGCCACT ATGGAACTTC TAGGGGACAT AGTCAGCCGC GCCAGCAGTA TCCTCAGCAT 
CACCAGCAAC AGTATTACGA TGAGCATGGC ATGGAGATGG GTCCACCGCC ACTGCCCCCA 
CATAAGAAGA AGAAATCGGT GCTGAAGAGC CCACTGGTGG CCCTCAAGAA TGCGCTGCTT 
AAGAGCACGC GGCCCCTGAG GAGAATGAAC AGCATGGTGG AGCCGGAGAG GAAACCGAAG 
GGACTCCGAC GCCAGCAATC GATGCTGGAG AGGGGTGTGC AAAGACCCTA CTATCCGGAT 
GAGTATCCAA CCTATCCAGC TGGCTTTGAG GAGCGTGCCC ATGGCGGACA AGGCATGATG 
CAGCCTCATC CGCAAGATGC CTACTATCAA AGGGGTGGTC ACTACACGTC GCAGCAGCAG 
GAGATGATGA ACAGCACCTA CCAGAATCTG GAGGGTGAGG ATATCTATGG GAACATCGGA 
AACACAGTTC CTAGAATGCC GCATCACCAG GACAATGGTT ACGGATATGA CCAATACGAT 
CTCTATGCCA ATCGAGCGTG CATCGATCTC GAAAGAAGGC AAGCAGAGGC AGCTGCAGCT 
TCCGGTGGCA GAAATGGGAG GAGAATTGTG AGGCGGCACA GCACCACCAC TGCGGATCGA 
GGAGGAAATC CCGGACCGCC CAGGAGGCCT ATGATCAGTC CTGGTTATGA GCAGGATCCG 
CAAGAGATCT ACCAGACCCG CAACGGAGCC TACATGCTGC CCGATCAGCG AAGGGCGCCC 
AGTTCGGAGG TGATGACCCG CAGAAGATTC TATCCGGGGG CTGCCAATGA GCAAACTGAG 
GAGGAGCCCC TCTACCAATC CCGTCGCGAA ATGCAGCGTG AGATGCAGAG GAATCATCTG 
TACCAATCGA AACGGGAAAT GCAGGAGAGG ATTAGCCAGG GCAAGAGGGA TATGGAGCGA 
GAGTTTAGCC CCCAGGGCAG CAGCAGCCAG TCGGAGACCT CCAATCCGGA GGTAATATAC 
CAGAGTCGCC GGGAGGCCAA GGAAAGTGCT CTGAAAACGA GGGCACAACT GAGGGATCAG 
ATCTATCAGA CTAGACGGGA AGCCCTGGAC AGCATGGCGG AACCGATCTA TGTGTCCAAA 
AGAGATATGG GCAGACCGGC TCCTATCTAT GAGACTCGGG AAGAGAGTAT CCTCCAGTCC 
CGAGAAAATG AAACCGACGA AAAGAAGGAA AAGGAGTGCA AGACCGAGCA GATTCAGGTG 
GAGATTAATG CTCAACCGGA GGAACAGGTG GAAGATTCGA CCTTAAGTCG ATCCGACTTG 
CAGAAATCTT CGGACACGGT GATAGAAAAT CCAGCGAGAG CTCCTCTTGT GCAGGATGAG 
GTAGATGACG ACGAAGAAGA GCAGGCTCAG GCTCGGGATG ATCCCGCTGA AAGCTCTGCG 
GATCAGACAG AGCTGCCAAC GGCCATTGAA AACAATCTAC ACAACGAGGG AATGGTGGAG 
CAAACACAGA ATGAGTCGGA TGACGTATTC GAAGCAGCCG ATAAGGTTTC TCCTCTAGCC 
GCTCCTACAG GTACTGTTCA ACCGCCGACC CCGCGATCCA GTAGAGCTCC CTTCCACATT 
TCGAATATCC TAAAGCGAAC AGCACCTCCT CCGAGTTCCC CAATGGGCGA CAGCTGCACC 
TCCATCGAAA CCCAGTACAC ATCCCAGGCC AGCCTGCCAG TGGGCCCACC AAATGCCACC 
AGTACGCCGT TCAGTAGCAG CATGTCATTG CCCATCGCCG GACCCGTAAA CAATCAACCG 
CCGGCCAGCG GACCCTTTCC CACTCTGCCC CGCGAACCCA GCACCTCGCG CGGATTCTTT 
GACTCCAATG GTGGCACTCT GGCAGATAAG CTGTGGCACG TCTCACTGCA AATCCCGCCG 
GGAGCCATTC CAGCCGGAGT ACGGCAAGAG ATCTACTTCA CCGTGAGCGA TCCGCGAATG 
GGACAAGCCG TCGGCGGTCC TCCGCTGGAC ATGGAAAATG GTGAGACAAT GCTGTCACCG 
CTGGTGATGT GCGGGCCGCA GGGCCTTGAG TTCCTGGTAC CGGTTACACT CAACATACCG 
CACTGCGCCG GACGAACTGC ATCACTGGGA CTGGCCCTCA AGGCGACCGA CAGCGAGAAG 
AACCTGCACA CCGAGTGGGA CAACATCGAC CTGCCCAGCA ATGCCGCCGC CCACACGGTC 
TCCGTCAAGG TGGATCACTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_026981926.1 ) cDNA ORF clone, Drosophila erecta -> XP_026837727.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X7, mRNA.

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CloneID	ODi33444
Clone ID Related Accession (Same CDS sequence)	XM_001980861.2 , XM_026981926.1
Accession Version	XM_026981926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCTCGACGC ACCGACGCTC GAAGAGTCTG 
TCCCGCGAGA ACTGGGACGA CGTGGTCTTC TCCGACAGCA TTTCCAAATT CCCCGCCTAC 
GAGCGAGTCG TTCTCCGCCA TCCTGGCTTT GTTCGACCCG TAGTGCTCTT CGGACCCGTA 
TCCGATCTGG CCCGCGAGCG CTTGGCCAAG GATTTCCCCG ACAAGTTTTC GACTCCACTG 
CAGGATGATG ACAAGTCAGC TGCAACCAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAC 
ATACGCGATG TGATGGATCG TGGCAAGCAC GCCCTGCTGG ACATAACGCC CAATGCAGTG 
GATCGTCTCA ACTACGCCCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG 
CACGTGATCA AGCAGCTGCG CCACGGACTA CCCAAGGCGG CCCACAAGAG CTCCAAGAAG 
CTGCTGGAGC AGACCCAGAA ACTGGAGCGC GTCTGGTCCC ACATCTTTAG CACCCAGATC 
GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ACTCGATCGA TCTGCAGCAG 
AGCGGCGCCG TGTGGATGTC CGAGTCCAAG CCAGTAGAAT CCCTCTCCGA CGATTTCCTA 
TTCCCCATGA CCACATCCCG ACTCTCGTAT GCATCAAGTC CCGAATCCGA TTTGGAACTG 
AGTCCCGGCC CATCCGCATC ATTGTCGCTT GGCAATTTGC CGCAGTTGGT TAAAGCGAGC 
TCAGATCCAT CGATTGCCAC TAATCAGGAT AACTTGGATA GGGATAGAGA CATAATTGGT 
GAAGGACTAC CACCTCCATA TACGGTACCC TACGACCATG GTGTCCCCGC GAATCCCAAT 
CGCCGCCAGA CCATGGACTC CAGCAAGTAC AGCATCTACG GCACCAACGT GCCGCCGCAG 
CCGCAGCAGC CCGGCGCAGG AGGAGATCCG GCTGCAGTGA GGCCACAGTC TCTGTACGGG 
ATTAATGCAC CCGATCTGCC GCCCCGCATC GATCGTCAGT CCAAGCCGGG TGATATGCCA 
CTGAACACCT CGGGCTCGTC GTCGCGAAAT GGCACCCTGG GTCGCAGTGC CCAGGAGCGT 
CTGTTTGGCA AGGCTGTGGT GCAGGACGAT GTCCAGGCGG AATACATAAC CCGAAATGCT 
CTGGTCGGCT CCGGGGCGGC TGAAACCCTG GATCGCCAGC AGCAGCAGCA AACCCACGCG 
TCCTTGGAGC GGCAGGCGCG TTTGAATGCG CAGCTGAAGG CCAACGGAGG AGGCGCCGGA 
GGAGGTGCAT CCACCTACGA CAGTGTGTCC AGCTACGACT CGTACAACAA CACACAAATG 
GCCATGCAAA ACCTGGGCCG ACTGGGTCCC AATGCACCCG ACGATCTCAA ATCAGTGCCA 
AATGCAAGTG GTCGCCCCTT GCCTCCGACG GGTCAGTCGC TCGACTACGG ACGCACTCCG 
CACGATCACC GCAGCTTCGG AGGAGCCAAC GATCTGAACC GACAGAGCAG TCCGGGCAGA 
CCGCACTACC ATGACATGAA TGCGTCCCGT AATATTGATC CACGCAATGG CACTCCACAG 
CGTCCTTCGA ATCTGGGCCT AGAAAGCAGT CCACGCAAGC CTTTGGTGGA GACGAAGACG 
GATTACGGCA AATACAGTCG AAACAACTCC GTATCGCAAG CGGATTACAC CAAGCTACCG 
AAGACGGCGC CACATGGAGT GGTACCGCCG CCGAATGTGA GCAACGGCCA AAGCCAGATG 
AATGGCAGTG GAACGCCCAG CAGCAATGGC AGTGGACCCT TCAAGCCGGT GCCGCCACCC 
AAACCCAAGA ACTATAGGCC ACCGGTGCAA AGTGGTGGCA GCAGTGGCAG CGGTGGCACT 
ACTCCGTGGG AAAATGGGGA CTCTGGCTCG CCACGCTCTC CAAATGGCTT CTATTATCCA 
CCAACGCCTT CGCATCATCA CTACGGCCAG CAGGCGACTC CCGGTTCACC CAGCAACGGC 
CACATGCAGC CACCTCCGCC GCAGCAGCAG CAGCAGCAGC CCACCTACGG CGGAAGTAAT 
GGCAACTACG GACAGGCGCC ACCACCACAA CCCTATCCGC AGGCCAATGG CTACAACGGG 
AATGGCCATC ACTACAACGG AGGCAGCGGC ACGGGACCCT ACATTGCACC ACATCGCGGC 
ATGCCGCCGC CAATAGGAAA CCTGCCGCCC CACACGCCCG AACGTCATGC CCTGGACTTG 
GCCGGAAGTC GGGAGCAGCG TGGCTCGGCC TTCGAGCTGT ATCGTAAGCC GCAAATCGGA 
GCAACCGCTG GGCACCATCA CAACATGAGC GAAATGGAGC CGTACGACGA GCGATACGAT 
GATTACTATA ACATGCCACC CCCTGCCGCC CATCCATCCC AAGGGCACCA TATGCAGCGC 
TCCCGATCAG CTCCTCGTTA TCCCCAGGAG CGACCTCCTC ACGCACAAGA TCCGAATTAC 
TACGGCCACT ATGGAACTTC TAGGGGACAT AGTCAGCCGC GCCAGCAGTA TCCTCAGCAT 
CACCAGCAAC AGTATTACGA TGAGCATGGC ATGGAGATGG GTCCACCGCC ACTGCCCCCA 
CATAAGAAGA AGAAATCGGT GCTGAAGAGC CCACTGGTGG CCCTCAAGAA TGCGCTGCTT 
AAGAGCACGC GGCCCCTGAG GAGAATGAAC AGCATGGTGG AGCCGGAGAG GAAACCGAAG 
GGACTCCGAC GCCAGCAATC GATGCTGGAG AGGGGTGTGC AAAGACCCTA CTATCCGGAT 
GAGTATCCAA CCTATCCAGC TGGCTTTGAG GAGCGTGCCC ATGGCGGACA AGGCATGATG 
CAGCCTCATC CGCAAGATGC CTACTATCAA AGGGGTGGTC ACTACACGTC GCAGCAGCAG 
GAGATGATGA ACAGCACCTA CCAGAATCTG GAGGGTGAGG ATATCTATGG GAACATCGGA 
AACACAGTTC CTAGAATGCC GCATCACCAG GACAATGGTT ACGGATATGA CCAATACGAT 
CTCTATGCCA ATCGAGCGTG CATCGATCTC GAAAGAAGGC AAGCAGAGGC AGCTGCAGCT 
TCCGGTGGCA GAAATGGGAG GAGAATTGTG AGGCGGCACA GCACCACCAC TGCGGATCGA 
GGAGGAAATC CCGGACCGCC CAGGAGGCCT ATGATCAGTC CTGGTTATGA GCAGGATCCG 
CAAGAGATCT ACCAGACCCG CAACGGAGCC TACATGCTGC CCGATCAGCG AAGGGCGCCC 
AGTTCGGAGG TGATGACCCG CAGAAGATTC TATCCGGGGG CTGCCAATGA GCAAACTGAG 
GAGGAGCCCC TCTACCAATC CCGTCGCGAA ATGCAGCGTG AGATGCAGAG GAATCATCTG 
TACCAATCGA AACGGGAAAT GCAGGAGAGG ATTAGCCAGG GCAAGAGGGA TATGGAGCGA 
GAGTTTAGCC CCCAGGGCAG CAGCAGCCAG TCGGAGACCT CCAATCCGGA GGTAATATAC 
CAGAGTCGCC GGGAGGCCAA GGAAAGTGCT CTGAAAACGA GGGCACAACT GAGGGATCAG 
ATCTATCAGA CTAGACGGGA AGCCCTGGAC AGCATGGCGG AACCGATCTA TGTGTCCAAA 
AGAGATATGG GCAGACCGGC TCCTATCTAT GAGACTCGGG AAGAGAGTAT CCTCCAGTCC 
CGAGAAAATG AAACCGACGA AAAGAAGGAA AAGGAGTGCA AGACCGAGCA GATTCAGGTG 
GAGATTAATG CTCAACCGGA GGAACAGGTG GAAGATTCGA CCTTAAGTCG ATCCGACTTG 
CAGAAATCTT CGGACACGGT GATAGAAAAT CCAGCGAGAG CTCCTCTTGT GCAGGATGAG 
GTAGATGACG ACGAAGAAGA GCAGGCTCAG GCTCGGGATG ATCCCGCTGA AAGCTCTGCG 
GATCAGACAG AGCTGCCAAC GGCCATTGAA AACAATCTAC ACAACGAGGG AATGGTGGAG 
CAAACACAGA ATGAGTCGGA TGACGTATTC GAAGCAGCCG ATAAGGTTTC TCCTCTAGCC 
GCTCCTACAG GTACTGTTCA ACCGCCGACC CCGCGATCCA GTAGAGCTCC CTTCCACATT 
TCGAATATCC TAAAGCGAAC AGCACCTCCT CCGAGTTCCC CAATGGGCGA CAGCTGCACC 
TCCATCGAAA CCCAGTACAC ATCCCAGGCC AGCCTGCCAG TGGGCCCACC AAATGCCACC 
AGTACGCCGT TCAGTAGCAG CATGTCATTG CCCATCGCCG GACCCGTAAA CAATCAACCG 
CCGGCCAGCG GACCCTTTCC CACTCTGCCC CGCGAACCCA GCACCTCGCG CGGATTCTTT 
GACTCCAATG GTGGCACTCT GGCAGATAAG CTGTGGCACG TCTCACTGCA AATCCCGCCG 
GGAGCCATTC CAGCCGGAGT ACGGCAAGAG ATCTACTTCA CCGTGAGCGA TCCGCGAATG 
GGACAAGCCG TCGGCGGTCC TCCGCTGGAC ATGGAAAATG GTGAGACAAT GCTGTCACCG 
CTGGTGATGT GCGGGCCGCA GGGCCTTGAG TTCCTGGTAC CGGTTACACT CAACATACCG 
CACTGCGCCG GACGAACTGC ATCACTGGGA CTGGCCCTCA AGGCGACCGA CAGCGAGAAG 
AACCTGCACA CCGAGTGGGA CAACATCGAC CTGCCCAGCA ATGCCGCCGC CCACACGGTC 
TCCGTCAAGG TGGATCACTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026837727.1
CDS	480..6983
Translation	MKFPDYVPNNMLVGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_026981926.1
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026981926.1

ORF Insert Sequence:

ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCTCGACGC ACCGACGCTC GAAGAGTCTG 
TCCCGCGAGA ACTGGGACGA CGTGGTCTTC TCCGACAGCA TTTCCAAATT CCCCGCCTAC 
GAGCGAGTCG TTCTCCGCCA TCCTGGCTTT GTTCGACCCG TAGTGCTCTT CGGACCCGTA 
TCCGATCTGG CCCGCGAGCG CTTGGCCAAG GATTTCCCCG ACAAGTTTTC GACTCCACTG 
CAGGATGATG ACAAGTCAGC TGCAACCAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAC 
ATACGCGATG TGATGGATCG TGGCAAGCAC GCCCTGCTGG ACATAACGCC CAATGCAGTG 
GATCGTCTCA ACTACGCCCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG 
CACGTGATCA AGCAGCTGCG CCACGGACTA CCCAAGGCGG CCCACAAGAG CTCCAAGAAG 
CTGCTGGAGC AGACCCAGAA ACTGGAGCGC GTCTGGTCCC ACATCTTTAG CACCCAGATC 
GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ACTCGATCGA TCTGCAGCAG 
AGCGGCGCCG TGTGGATGTC CGAGTCCAAG CCAGTAGAAT CCCTCTCCGA CGATTTCCTA 
TTCCCCATGA CCACATCCCG ACTCTCGTAT GCATCAAGTC CCGAATCCGA TTTGGAACTG 
AGTCCCGGCC CATCCGCATC ATTGTCGCTT GGCAATTTGC CGCAGTTGGT TAAAGCGAGC 
TCAGATCCAT CGATTGCCAC TAATCAGGAT AACTTGGATA GGGATAGAGA CATAATTGGT 
GAAGGACTAC CACCTCCATA TACGGTACCC TACGACCATG GTGTCCCCGC GAATCCCAAT 
CGCCGCCAGA CCATGGACTC CAGCAAGTAC AGCATCTACG GCACCAACGT GCCGCCGCAG 
CCGCAGCAGC CCGGCGCAGG AGGAGATCCG GCTGCAGTGA GGCCACAGTC TCTGTACGGG 
ATTAATGCAC CCGATCTGCC GCCCCGCATC GATCGTCAGT CCAAGCCGGG TGATATGCCA 
CTGAACACCT CGGGCTCGTC GTCGCGAAAT GGCACCCTGG GTCGCAGTGC CCAGGAGCGT 
CTGTTTGGCA AGGCTGTGGT GCAGGACGAT GTCCAGGCGG AATACATAAC CCGAAATGCT 
CTGGTCGGCT CCGGGGCGGC TGAAACCCTG GATCGCCAGC AGCAGCAGCA AACCCACGCG 
TCCTTGGAGC GGCAGGCGCG TTTGAATGCG CAGCTGAAGG CCAACGGAGG AGGCGCCGGA 
GGAGGTGCAT CCACCTACGA CAGTGTGTCC AGCTACGACT CGTACAACAA CACACAAATG 
GCCATGCAAA ACCTGGGCCG ACTGGGTCCC AATGCACCCG ACGATCTCAA ATCAGTGCCA 
AATGCAAGTG GTCGCCCCTT GCCTCCGACG GGTCAGTCGC TCGACTACGG ACGCACTCCG 
CACGATCACC GCAGCTTCGG AGGAGCCAAC GATCTGAACC GACAGAGCAG TCCGGGCAGA 
CCGCACTACC ATGACATGAA TGCGTCCCGT AATATTGATC CACGCAATGG CACTCCACAG 
CGTCCTTCGA ATCTGGGCCT AGAAAGCAGT CCACGCAAGC CTTTGGTGGA GACGAAGACG 
GATTACGGCA AATACAGTCG AAACAACTCC GTATCGCAAG CGGATTACAC CAAGCTACCG 
AAGACGGCGC CACATGGAGT GGTACCGCCG CCGAATGTGA GCAACGGCCA AAGCCAGATG 
AATGGCAGTG GAACGCCCAG CAGCAATGGC AGTGGACCCT TCAAGCCGGT GCCGCCACCC 
AAACCCAAGA ACTATAGGCC ACCGGTGCAA AGTGGTGGCA GCAGTGGCAG CGGTGGCACT 
ACTCCGTGGG AAAATGGGGA CTCTGGCTCG CCACGCTCTC CAAATGGCTT CTATTATCCA 
CCAACGCCTT CGCATCATCA CTACGGCCAG CAGGCGACTC CCGGTTCACC CAGCAACGGC 
CACATGCAGC CACCTCCGCC GCAGCAGCAG CAGCAGCAGC CCACCTACGG CGGAAGTAAT 
GGCAACTACG GACAGGCGCC ACCACCACAA CCCTATCCGC AGGCCAATGG CTACAACGGG 
AATGGCCATC ACTACAACGG AGGCAGCGGC ACGGGACCCT ACATTGCACC ACATCGCGGC 
ATGCCGCCGC CAATAGGAAA CCTGCCGCCC CACACGCCCG AACGTCATGC CCTGGACTTG 
GCCGGAAGTC GGGAGCAGCG TGGCTCGGCC TTCGAGCTGT ATCGTAAGCC GCAAATCGGA 
GCAACCGCTG GGCACCATCA CAACATGAGC GAAATGGAGC CGTACGACGA GCGATACGAT 
GATTACTATA ACATGCCACC CCCTGCCGCC CATCCATCCC AAGGGCACCA TATGCAGCGC 
TCCCGATCAG CTCCTCGTTA TCCCCAGGAG CGACCTCCTC ACGCACAAGA TCCGAATTAC 
TACGGCCACT ATGGAACTTC TAGGGGACAT AGTCAGCCGC GCCAGCAGTA TCCTCAGCAT 
CACCAGCAAC AGTATTACGA TGAGCATGGC ATGGAGATGG GTCCACCGCC ACTGCCCCCA 
CATAAGAAGA AGAAATCGGT GCTGAAGAGC CCACTGGTGG CCCTCAAGAA TGCGCTGCTT 
AAGAGCACGC GGCCCCTGAG GAGAATGAAC AGCATGGTGG AGCCGGAGAG GAAACCGAAG 
GGACTCCGAC GCCAGCAATC GATGCTGGAG AGGGGTGTGC AAAGACCCTA CTATCCGGAT 
GAGTATCCAA CCTATCCAGC TGGCTTTGAG GAGCGTGCCC ATGGCGGACA AGGCATGATG 
CAGCCTCATC CGCAAGATGC CTACTATCAA AGGGGTGGTC ACTACACGTC GCAGCAGCAG 
GAGATGATGA ACAGCACCTA CCAGAATCTG GAGGGTGAGG ATATCTATGG GAACATCGGA 
AACACAGTTC CTAGAATGCC GCATCACCAG GACAATGGTT ACGGATATGA CCAATACGAT 
CTCTATGCCA ATCGAGCGTG CATCGATCTC GAAAGAAGGC AAGCAGAGGC AGCTGCAGCT 
TCCGGTGGCA GAAATGGGAG GAGAATTGTG AGGCGGCACA GCACCACCAC TGCGGATCGA 
GGAGGAAATC CCGGACCGCC CAGGAGGCCT ATGATCAGTC CTGGTTATGA GCAGGATCCG 
CAAGAGATCT ACCAGACCCG CAACGGAGCC TACATGCTGC CCGATCAGCG AAGGGCGCCC 
AGTTCGGAGG TGATGACCCG CAGAAGATTC TATCCGGGGG CTGCCAATGA GCAAACTGAG 
GAGGAGCCCC TCTACCAATC CCGTCGCGAA ATGCAGCGTG AGATGCAGAG GAATCATCTG 
TACCAATCGA AACGGGAAAT GCAGGAGAGG ATTAGCCAGG GCAAGAGGGA TATGGAGCGA 
GAGTTTAGCC CCCAGGGCAG CAGCAGCCAG TCGGAGACCT CCAATCCGGA GGTAATATAC 
CAGAGTCGCC GGGAGGCCAA GGAAAGTGCT CTGAAAACGA GGGCACAACT GAGGGATCAG 
ATCTATCAGA CTAGACGGGA AGCCCTGGAC AGCATGGCGG AACCGATCTA TGTGTCCAAA 
AGAGATATGG GCAGACCGGC TCCTATCTAT GAGACTCGGG AAGAGAGTAT CCTCCAGTCC 
CGAGAAAATG AAACCGACGA AAAGAAGGAA AAGGAGTGCA AGACCGAGCA GATTCAGGTG 
GAGATTAATG CTCAACCGGA GGAACAGGTG GAAGATTCGA CCTTAAGTCG ATCCGACTTG 
CAGAAATCTT CGGACACGGT GATAGAAAAT CCAGCGAGAG CTCCTCTTGT GCAGGATGAG 
GTAGATGACG ACGAAGAAGA GCAGGCTCAG GCTCGGGATG ATCCCGCTGA AAGCTCTGCG 
GATCAGACAG AGCTGCCAAC GGCCATTGAA AACAATCTAC ACAACGAGGG AATGGTGGAG 
CAAACACAGA ATGAGTCGGA TGACGTATTC GAAGCAGCCG ATAAGGTTTC TCCTCTAGCC 
GCTCCTACAG GTACTGTTCA ACCGCCGACC CCGCGATCCA GTAGAGCTCC CTTCCACATT 
TCGAATATCC TAAAGCGAAC AGCACCTCCT CCGAGTTCCC CAATGGGCGA CAGCTGCACC 
TCCATCGAAA CCCAGTACAC ATCCCAGGCC AGCCTGCCAG TGGGCCCACC AAATGCCACC 
AGTACGCCGT TCAGTAGCAG CATGTCATTG CCCATCGCCG GACCCGTAAA CAATCAACCG 
CCGGCCAGCG GACCCTTTCC CACTCTGCCC CGCGAACCCA GCACCTCGCG CGGATTCTTT 
GACTCCAATG GTGGCACTCT GGCAGATAAG CTGTGGCACG TCTCACTGCA AATCCCGCCG 
GGAGCCATTC CAGCCGGAGT ACGGCAAGAG ATCTACTTCA CCGTGAGCGA TCCGCGAATG 
GGACAAGCCG TCGGCGGTCC TCCGCTGGAC ATGGAAAATG GTGAGACAAT GCTGTCACCG 
CTGGTGATGT GCGGGCCGCA GGGCCTTGAG TTCCTGGTAC CGGTTACACT CAACATACCG 
CACTGCGCCG GACGAACTGC ATCACTGGGA CTGGCCCTCA AGGCGACCGA CAGCGAGAAG 
AACCTGCACA CCGAGTGGGA CAACATCGAC CTGCCCAGCA ATGCCGCCGC CCACACGGTC 
TCCGTCAAGG TGGATCACTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154540.2 ) cDNA ORF clone, Drosophila erecta -> XP_015010026.1 Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X2, mRNA.

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CloneID	ODi33927
Clone ID Related Accession (Same CDS sequence)	XM_015154540.2 , XM_015154540.1
Accession Version	XM_015154540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015154540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGCCAGTAGA ATCCCTCTCC 
GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC 
GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG 
GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA 
GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGGTGTCCCC 
GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAC 
GTGCCGCCGC AGCCGCAGCA GCCCGGCGCA GGAGGAGATC CGGCTGCAGT GAGGCCACAG 
TCTCTGTACG GGATTAATGC ACCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG 
GGTGATATGC CACTGAACAC CTCGGGCTCG TCGTCGCGAA ATGGCACCCT GGGTCGCAGT 
GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAATACATA 
ACCCGAAATG CTCTGGTCGG CTCCGGGGCG GCTGAAACCC TGGATCGCCA GCAGCAGCAG 
CAAACCCACG CGTCCTTGGA GCGGCAGGCG CGTTTGAATG CGCAGCTGAA GGCCAACGGA 
GGAGGCGCCG GAGGAGGTGC ATCCACCTAC GACAGTGTGT CCAGCTACGA CTCGTACAAC 
AACACACAAA TGGCCATGCA AAACCTGGGC CGACTGGGTC CCAATGCACC CGACGATCTC 
AAATCAGTGC CAAATGCAAG TGGTCGCCCC TTGCCTCCGA CGGGTCAGTC GCTCGACTAC 
GGACGCACTC CGCACGATCA CCGCAGCTTC GGAGGAGCCA ACGATCTGAA CCGACAGAGC 
AGTCCGGGCA GACCGCACTA CCATGACATG AATGCGTCCC GTAATATTGA TCCACGCAAT 
GGCACTCCAC AGCGTCCTTC GAATCTGGGC CTAGAAAGCA GTCCACGCAA GCCTTTGGTG 
GAGACGAAGA CGGATTACGG CAAATACAGT CGAAACAACT CCGTATCGCA AGCGGATTAC 
ACCAAGCTAC CGAAGACGGC GCCACATGGA GTGGTACCGC CGCCGAATGT GAGCAACGGC 
CAAAGCCAGA TGAATGGCAG TGGAACGCCC AGCAGCAATG GCAGTGGACC CTTCAAGCCG 
GTGCCGCCAC CCAAACCCAA GAACTATAGG CCACCGGTGC AAAGTGGTGG CAGCAGTGGC 
AGCGGTGGCA CTACTCCGTG GGAAAATGGG GACTCTGGCT CGCCACGCTC TCCAAATGGC 
TTCTATTATC CACCAACGCC TTCGCATCAT CACTACGGCC AGCAGGCGAC TCCCGGTTCA 
CCCAGCAACG GCCACATGCA GCCACCTCCG CCGCAGCAGC AGCAGCAGCA GCCCACCTAC 
GGCGGAAGTA ATGGCAACTA CGGACAGGCG CCACCACCAC AACCCTATCC GCAGGCCAAT 
GGCTACAACG GGAATGGCCA TCACTACAAC GGAGGCAGCG GCACGGGACC CTACATTGCA 
CCACATCGCG GCATGCCGCC GCCAATAGGA AACCTGCCGC CCCACACGCC CGAACGTCAT 
GCCCTGGACT TGGCCGGAAG TCGGGAGCAG CGTGGCTCGG CCTTCGAGCT GTATCGTAAG 
CCGCAAATCG GAGCAACCGC TGGGCACCAT CACAACATGA GCGAAATGGA GCCGTACGAC 
GAGCGATACG ATGATTACTA TAACATGCCA CCCCCTGCCG CCCATCCATC CCAAGGGCAC 
CATATGCAGC GCTCCCGATC AGCTCCTCGT TATCCCCAGG AGCGACCTCC TCACGCACAA 
GATCCGAATT ACTACGGCCA CTATGGAACT TCTAGGGGAC ATAGTCAGCC GCGCCAGCAG 
TATCCTCAGC ATCACCAGCA ACAGTATTAC GATGAGCATG GCATGGAGAT GGGTCCACCG 
CCACTGCCCC CACATAAGAA GAAGAAATCG GTGCTGAAGA GCCCACTGGT GGCCCTCAAG 
AATGCGCTGC TTAAGAGCAC GCGGCCCCTG AGGAGAATGA ACAGCATGGT GGAGCCGGAG 
AGGAAACCGA AGGGACTCCG ACGCCAGCAA TCGATGCTGG AGAGGGGTGT GCAAAGACCC 
TACTATCCGG ATGAGTATCC AACCTATCCA GCTGGCTTTG AGGAGCGTGC CCATGGCGGA 
CAAGGCATGA TGCAGCCTCA TCCGCAAGAT GCCTACTATC AAAGGGGTGG TCACTACACG 
TCGCAGCAGC AGGAGATGAT GAACAGCACC TACCAGAATC TGGAGGGTGA GGATATCTAT 
GGGAACATCG GAAACACAGT TCCTAGAATG CCGCATCACC AGGACAATGG TTACGGATAT 
GACCAATACG ATCTCTATGC CAATCGAGCG TGCATCGATC TCGAAAGAAG GCAAGCAGAG 
GCAGCTGCAG CTTCCGGTGG CAGAAATGGG AGGAGAATTG TGAGGCGGCA CAGCACCACC 
ACTGCGGATC GAGGAGGAAA TCCCGGACCG CCCAGGAGGC CTATGATCAG TCCTGGTTAT 
GAGCAGGATC CGCAAGAGAT CTACCAGACC CGCAACGGAG CCTACATGCT GCCCGATCAG 
CGAAGGGCGC CCAGTTCGGA GGTGATGACC CGCAGAAGAT TCTATCCGGG GGCTGCCAAT 
GAGCAAACTG AGGAGGAGCC CCTCTACCAA TCCCGTCGCG AAATGCAGCG TGAGATGCAG 
AGGAATCATC TGTACCAATC GAAACGGGAA ATGCAGGAGA GGATTAGCCA GGGCAAGAGG 
GATATGGAGC GAGAGTTTAG CCCCCAGGGC AGCAGCAGCC AGTCGGAGAC CTCCAATCCG 
GAGGTAATAT ACCAGAGTCG CCGGGAGGCC AAGGAAAGTG CTCTGAAAAC GAGGGCACAA 
CTGAGGGATC AGATCTATCA GACTAGACGG GAAGCCCTGG ACAGCATGGC GGAACCGATC 
TATGTGTCCA AAAGAGATAT GGGCAGACCG GCTCCTATCT ATGAGACTCG GGAAGAGAGT 
ATCCTCCAGT CCCGAGAAAA TGAAACCGAC GAAAAGAAGG AAAAGGAGTG CAAGACCGAG 
CAGATTCAGG TGGAGATTAA TGCTCAACCG GAGGAACAGG TGGAAGATTC GACCTTAAGT 
CGATCCGACT TGCAGAAATC TTCGGACACG GTGATAGAAA ATCCAGCGAG AGCTCCTCTT 
GTGCAGGATG AGGTAGATGA CGACGAAGAA GAGCAGGCTC AGGCTCGGGA TGATCCCGCT 
GAAAGCTCTG CGGATCAGAC AGAGCTGCCA ACGGCCATTG AAAACAATCT ACACAACGAG 
GGAATGGTGG AGCAAACACA GAATGAGTCG GATGACGTAT TCGAAGCAGC CGATAAGGTT 
TCTCCTCTAG CCGCTCCTAC AGGTACTGTT CAACCGCCGA CCCCGCGATC CAGTAGAGCT 
CCCTTCCACA TTTCGAATAT CCTAAAGCGA ACAGCACCTC CTCCGAGTTC CCCAATGGGC 
GACAGCTGCA CCTCCATCGA AACCCAGTAC ACATCCCAGG CCAGCCTGCC AGTGGGCCCA 
CCAAATGCCA CCAGTACGCC GTTCAGTAGC AGCATGTCAT TGCCCATCGC CGGACCCGTA 
AACAATCAAC CGCCGGCCAG CGGACCCTTT CCCACTCTGC CCCGCGAACC CAGCACCTCG 
CGCGGATTCT TTGACTCCAA TGGTGGCACT CTGGCAGATA AGCTGTGGCA CGTCTCACTG 
CAAATCCCGC CGGGAGCCAT TCCAGCCGGA GTACGGCAAG AGATCTACTT CACCGTGAGC 
GATCCGCGAA TGGGACAAGC CGTCGGCGGT CCTCCGCTGG ACATGGAAAA TGGTGAGACA 
ATGCTGTCAC CGCTGGTGAT GTGCGGGCCG CAGGGCCTTG AGTTCCTGGT ACCGGTTACA 
CTCAACATAC CGCACTGCGC CGGACGAACT GCATCACTGG GACTGGCCCT CAAGGCGACC 
GACAGCGAGA AGAACCTGCA CACCGAGTGG GACAACATCG ACCTGCCCAG CAATGCCGCC 
GCCCACACGG TCTCCGTCAA GGTGGATCAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010026.1
CDS	652..8247
Translation	MTREEEEEVRSLLSRHQERIMLDLGSTDLLAVLVKNSVLSQSEEDLLLKPYNAVTPTPSAAASATVEPPGSAPSLTKRKLSAVASSGSTASGGSAGSGGSGSSRCASVNGDANCDSRSLTPSGGGPCSVLNDAEILRVQCSNLIEIIAKNGFEKFKQFCYAIECECPQLIEDLINDRLKTDAANLEDSRDGEKMQKEIETIDYIDKEKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_015154540.2
Organism	Drosophila erecta
Definition	Drosophila erecta tight junction protein ZO-1 (LOC6552588), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154540.2

ORF Insert Sequence:

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGCCAGTAGA ATCCCTCTCC 
GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC 
GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG 
GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA 
GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGGTGTCCCC 
GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAC 
GTGCCGCCGC AGCCGCAGCA GCCCGGCGCA GGAGGAGATC CGGCTGCAGT GAGGCCACAG 
TCTCTGTACG GGATTAATGC ACCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG 
GGTGATATGC CACTGAACAC CTCGGGCTCG TCGTCGCGAA ATGGCACCCT GGGTCGCAGT 
GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAATACATA 
ACCCGAAATG CTCTGGTCGG CTCCGGGGCG GCTGAAACCC TGGATCGCCA GCAGCAGCAG 
CAAACCCACG CGTCCTTGGA GCGGCAGGCG CGTTTGAATG CGCAGCTGAA GGCCAACGGA 
GGAGGCGCCG GAGGAGGTGC ATCCACCTAC GACAGTGTGT CCAGCTACGA CTCGTACAAC 
AACACACAAA TGGCCATGCA AAACCTGGGC CGACTGGGTC CCAATGCACC CGACGATCTC 
AAATCAGTGC CAAATGCAAG TGGTCGCCCC TTGCCTCCGA CGGGTCAGTC GCTCGACTAC 
GGACGCACTC CGCACGATCA CCGCAGCTTC GGAGGAGCCA ACGATCTGAA CCGACAGAGC 
AGTCCGGGCA GACCGCACTA CCATGACATG AATGCGTCCC GTAATATTGA TCCACGCAAT 
GGCACTCCAC AGCGTCCTTC GAATCTGGGC CTAGAAAGCA GTCCACGCAA GCCTTTGGTG 
GAGACGAAGA CGGATTACGG CAAATACAGT CGAAACAACT CCGTATCGCA AGCGGATTAC 
ACCAAGCTAC CGAAGACGGC GCCACATGGA GTGGTACCGC CGCCGAATGT GAGCAACGGC 
CAAAGCCAGA TGAATGGCAG TGGAACGCCC AGCAGCAATG GCAGTGGACC CTTCAAGCCG 
GTGCCGCCAC CCAAACCCAA GAACTATAGG CCACCGGTGC AAAGTGGTGG CAGCAGTGGC 
AGCGGTGGCA CTACTCCGTG GGAAAATGGG GACTCTGGCT CGCCACGCTC TCCAAATGGC 
TTCTATTATC CACCAACGCC TTCGCATCAT CACTACGGCC AGCAGGCGAC TCCCGGTTCA 
CCCAGCAACG GCCACATGCA GCCACCTCCG CCGCAGCAGC AGCAGCAGCA GCCCACCTAC 
GGCGGAAGTA ATGGCAACTA CGGACAGGCG CCACCACCAC AACCCTATCC GCAGGCCAAT 
GGCTACAACG GGAATGGCCA TCACTACAAC GGAGGCAGCG GCACGGGACC CTACATTGCA 
CCACATCGCG GCATGCCGCC GCCAATAGGA AACCTGCCGC CCCACACGCC CGAACGTCAT 
GCCCTGGACT TGGCCGGAAG TCGGGAGCAG CGTGGCTCGG CCTTCGAGCT GTATCGTAAG 
CCGCAAATCG GAGCAACCGC TGGGCACCAT CACAACATGA GCGAAATGGA GCCGTACGAC 
GAGCGATACG ATGATTACTA TAACATGCCA CCCCCTGCCG CCCATCCATC CCAAGGGCAC 
CATATGCAGC GCTCCCGATC AGCTCCTCGT TATCCCCAGG AGCGACCTCC TCACGCACAA 
GATCCGAATT ACTACGGCCA CTATGGAACT TCTAGGGGAC ATAGTCAGCC GCGCCAGCAG 
TATCCTCAGC ATCACCAGCA ACAGTATTAC GATGAGCATG GCATGGAGAT GGGTCCACCG 
CCACTGCCCC CACATAAGAA GAAGAAATCG GTGCTGAAGA GCCCACTGGT GGCCCTCAAG 
AATGCGCTGC TTAAGAGCAC GCGGCCCCTG AGGAGAATGA ACAGCATGGT GGAGCCGGAG 
AGGAAACCGA AGGGACTCCG ACGCCAGCAA TCGATGCTGG AGAGGGGTGT GCAAAGACCC 
TACTATCCGG ATGAGTATCC AACCTATCCA GCTGGCTTTG AGGAGCGTGC CCATGGCGGA 
CAAGGCATGA TGCAGCCTCA TCCGCAAGAT GCCTACTATC AAAGGGGTGG TCACTACACG 
TCGCAGCAGC AGGAGATGAT GAACAGCACC TACCAGAATC TGGAGGGTGA GGATATCTAT 
GGGAACATCG GAAACACAGT TCCTAGAATG CCGCATCACC AGGACAATGG TTACGGATAT 
GACCAATACG ATCTCTATGC CAATCGAGCG TGCATCGATC TCGAAAGAAG GCAAGCAGAG 
GCAGCTGCAG CTTCCGGTGG CAGAAATGGG AGGAGAATTG TGAGGCGGCA CAGCACCACC 
ACTGCGGATC GAGGAGGAAA TCCCGGACCG CCCAGGAGGC CTATGATCAG TCCTGGTTAT 
GAGCAGGATC CGCAAGAGAT CTACCAGACC CGCAACGGAG CCTACATGCT GCCCGATCAG 
CGAAGGGCGC CCAGTTCGGA GGTGATGACC CGCAGAAGAT TCTATCCGGG GGCTGCCAAT 
GAGCAAACTG AGGAGGAGCC CCTCTACCAA TCCCGTCGCG AAATGCAGCG TGAGATGCAG 
AGGAATCATC TGTACCAATC GAAACGGGAA ATGCAGGAGA GGATTAGCCA GGGCAAGAGG 
GATATGGAGC GAGAGTTTAG CCCCCAGGGC AGCAGCAGCC AGTCGGAGAC CTCCAATCCG 
GAGGTAATAT ACCAGAGTCG CCGGGAGGCC AAGGAAAGTG CTCTGAAAAC GAGGGCACAA 
CTGAGGGATC AGATCTATCA GACTAGACGG GAAGCCCTGG ACAGCATGGC GGAACCGATC 
TATGTGTCCA AAAGAGATAT GGGCAGACCG GCTCCTATCT ATGAGACTCG GGAAGAGAGT 
ATCCTCCAGT CCCGAGAAAA TGAAACCGAC GAAAAGAAGG AAAAGGAGTG CAAGACCGAG 
CAGATTCAGG TGGAGATTAA TGCTCAACCG GAGGAACAGG TGGAAGATTC GACCTTAAGT 
CGATCCGACT TGCAGAAATC TTCGGACACG GTGATAGAAA ATCCAGCGAG AGCTCCTCTT 
GTGCAGGATG AGGTAGATGA CGACGAAGAA GAGCAGGCTC AGGCTCGGGA TGATCCCGCT 
GAAAGCTCTG CGGATCAGAC AGAGCTGCCA ACGGCCATTG AAAACAATCT ACACAACGAG 
GGAATGGTGG AGCAAACACA GAATGAGTCG GATGACGTAT TCGAAGCAGC CGATAAGGTT 
TCTCCTCTAG CCGCTCCTAC AGGTACTGTT CAACCGCCGA CCCCGCGATC CAGTAGAGCT 
CCCTTCCACA TTTCGAATAT CCTAAAGCGA ACAGCACCTC CTCCGAGTTC CCCAATGGGC 
GACAGCTGCA CCTCCATCGA AACCCAGTAC ACATCCCAGG CCAGCCTGCC AGTGGGCCCA 
CCAAATGCCA CCAGTACGCC GTTCAGTAGC AGCATGTCAT TGCCCATCGC CGGACCCGTA 
AACAATCAAC CGCCGGCCAG CGGACCCTTT CCCACTCTGC CCCGCGAACC CAGCACCTCG 
CGCGGATTCT TTGACTCCAA TGGTGGCACT CTGGCAGATA AGCTGTGGCA CGTCTCACTG 
CAAATCCCGC CGGGAGCCAT TCCAGCCGGA GTACGGCAAG AGATCTACTT CACCGTGAGC 
GATCCGCGAA TGGGACAAGC CGTCGGCGGT CCTCCGCTGG ACATGGAAAA TGGTGAGACA 
ATGCTGTCAC CGCTGGTGAT GTGCGGGCCG CAGGGCCTTG AGTTCCTGGT ACCGGTTACA 
CTCAACATAC CGCACTGCGC CGGACGAACT GCATCACTGG GACTGGCCCT CAAGGCGACC 
GACAGCGAGA AGAACCTGCA CACCGAGTGG GACAACATCG ACCTGCCCAG CAATGCCGCC 
GCCCACACGG TCTCCGTCAA GGTGGATCAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154540.1 ) cDNA ORF clone, Drosophila erecta -> XP_015010026.1 Drosophila erecta uncharacterized protein, transcript variant B (Dere\GG17413), mRNA.

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CloneID	ODi33927
Clone ID Related Accession (Same CDS sequence)	XM_015154540.2 , XM_015154540.1
Accession Version	XM_015154540.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956552). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGCCAGTAGA ATCCCTCTCC 
GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC 
GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG 
GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA 
GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGGTGTCCCC 
GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAC 
GTGCCGCCGC AGCCGCAGCA GCCCGGCGCA GGAGGAGATC CGGCTGCAGT GAGGCCACAG 
TCTCTGTACG GGATTAATGC ACCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG 
GGTGATATGC CACTGAACAC CTCGGGCTCG TCGTCGCGAA ATGGCACCCT GGGTCGCAGT 
GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAATACATA 
ACCCGAAATG CTCTGGTCGG CTCCGGGGCG GCTGAAACCC TGGATCGCCA GCAGCAGCAG 
CAAACCCACG CGTCCTTGGA GCGGCAGGCG CGTTTGAATG CGCAGCTGAA GGCCAACGGA 
GGAGGCGCCG GAGGAGGTGC ATCCACCTAC GACAGTGTGT CCAGCTACGA CTCGTACAAC 
AACACACAAA TGGCCATGCA AAACCTGGGC CGACTGGGTC CCAATGCACC CGACGATCTC 
AAATCAGTGC CAAATGCAAG TGGTCGCCCC TTGCCTCCGA CGGGTCAGTC GCTCGACTAC 
GGACGCACTC CGCACGATCA CCGCAGCTTC GGAGGAGCCA ACGATCTGAA CCGACAGAGC 
AGTCCGGGCA GACCGCACTA CCATGACATG AATGCGTCCC GTAATATTGA TCCACGCAAT 
GGCACTCCAC AGCGTCCTTC GAATCTGGGC CTAGAAAGCA GTCCACGCAA GCCTTTGGTG 
GAGACGAAGA CGGATTACGG CAAATACAGT CGAAACAACT CCGTATCGCA AGCGGATTAC 
ACCAAGCTAC CGAAGACGGC GCCACATGGA GTGGTACCGC CGCCGAATGT GAGCAACGGC 
CAAAGCCAGA TGAATGGCAG TGGAACGCCC AGCAGCAATG GCAGTGGACC CTTCAAGCCG 
GTGCCGCCAC CCAAACCCAA GAACTATAGG CCACCGGTGC AAAGTGGTGG CAGCAGTGGC 
AGCGGTGGCA CTACTCCGTG GGAAAATGGG GACTCTGGCT CGCCACGCTC TCCAAATGGC 
TTCTATTATC CACCAACGCC TTCGCATCAT CACTACGGCC AGCAGGCGAC TCCCGGTTCA 
CCCAGCAACG GCCACATGCA GCCACCTCCG CCGCAGCAGC AGCAGCAGCA GCCCACCTAC 
GGCGGAAGTA ATGGCAACTA CGGACAGGCG CCACCACCAC AACCCTATCC GCAGGCCAAT 
GGCTACAACG GGAATGGCCA TCACTACAAC GGAGGCAGCG GCACGGGACC CTACATTGCA 
CCACATCGCG GCATGCCGCC GCCAATAGGA AACCTGCCGC CCCACACGCC CGAACGTCAT 
GCCCTGGACT TGGCCGGAAG TCGGGAGCAG CGTGGCTCGG CCTTCGAGCT GTATCGTAAG 
CCGCAAATCG GAGCAACCGC TGGGCACCAT CACAACATGA GCGAAATGGA GCCGTACGAC 
GAGCGATACG ATGATTACTA TAACATGCCA CCCCCTGCCG CCCATCCATC CCAAGGGCAC 
CATATGCAGC GCTCCCGATC AGCTCCTCGT TATCCCCAGG AGCGACCTCC TCACGCACAA 
GATCCGAATT ACTACGGCCA CTATGGAACT TCTAGGGGAC ATAGTCAGCC GCGCCAGCAG 
TATCCTCAGC ATCACCAGCA ACAGTATTAC GATGAGCATG GCATGGAGAT GGGTCCACCG 
CCACTGCCCC CACATAAGAA GAAGAAATCG GTGCTGAAGA GCCCACTGGT GGCCCTCAAG 
AATGCGCTGC TTAAGAGCAC GCGGCCCCTG AGGAGAATGA ACAGCATGGT GGAGCCGGAG 
AGGAAACCGA AGGGACTCCG ACGCCAGCAA TCGATGCTGG AGAGGGGTGT GCAAAGACCC 
TACTATCCGG ATGAGTATCC AACCTATCCA GCTGGCTTTG AGGAGCGTGC CCATGGCGGA 
CAAGGCATGA TGCAGCCTCA TCCGCAAGAT GCCTACTATC AAAGGGGTGG TCACTACACG 
TCGCAGCAGC AGGAGATGAT GAACAGCACC TACCAGAATC TGGAGGGTGA GGATATCTAT 
GGGAACATCG GAAACACAGT TCCTAGAATG CCGCATCACC AGGACAATGG TTACGGATAT 
GACCAATACG ATCTCTATGC CAATCGAGCG TGCATCGATC TCGAAAGAAG GCAAGCAGAG 
GCAGCTGCAG CTTCCGGTGG CAGAAATGGG AGGAGAATTG TGAGGCGGCA CAGCACCACC 
ACTGCGGATC GAGGAGGAAA TCCCGGACCG CCCAGGAGGC CTATGATCAG TCCTGGTTAT 
GAGCAGGATC CGCAAGAGAT CTACCAGACC CGCAACGGAG CCTACATGCT GCCCGATCAG 
CGAAGGGCGC CCAGTTCGGA GGTGATGACC CGCAGAAGAT TCTATCCGGG GGCTGCCAAT 
GAGCAAACTG AGGAGGAGCC CCTCTACCAA TCCCGTCGCG AAATGCAGCG TGAGATGCAG 
AGGAATCATC TGTACCAATC GAAACGGGAA ATGCAGGAGA GGATTAGCCA GGGCAAGAGG 
GATATGGAGC GAGAGTTTAG CCCCCAGGGC AGCAGCAGCC AGTCGGAGAC CTCCAATCCG 
GAGGTAATAT ACCAGAGTCG CCGGGAGGCC AAGGAAAGTG CTCTGAAAAC GAGGGCACAA 
CTGAGGGATC AGATCTATCA GACTAGACGG GAAGCCCTGG ACAGCATGGC GGAACCGATC 
TATGTGTCCA AAAGAGATAT GGGCAGACCG GCTCCTATCT ATGAGACTCG GGAAGAGAGT 
ATCCTCCAGT CCCGAGAAAA TGAAACCGAC GAAAAGAAGG AAAAGGAGTG CAAGACCGAG 
CAGATTCAGG TGGAGATTAA TGCTCAACCG GAGGAACAGG TGGAAGATTC GACCTTAAGT 
CGATCCGACT TGCAGAAATC TTCGGACACG GTGATAGAAA ATCCAGCGAG AGCTCCTCTT 
GTGCAGGATG AGGTAGATGA CGACGAAGAA GAGCAGGCTC AGGCTCGGGA TGATCCCGCT 
GAAAGCTCTG CGGATCAGAC AGAGCTGCCA ACGGCCATTG AAAACAATCT ACACAACGAG 
GGAATGGTGG AGCAAACACA GAATGAGTCG GATGACGTAT TCGAAGCAGC CGATAAGGTT 
TCTCCTCTAG CCGCTCCTAC AGGTACTGTT CAACCGCCGA CCCCGCGATC CAGTAGAGCT 
CCCTTCCACA TTTCGAATAT CCTAAAGCGA ACAGCACCTC CTCCGAGTTC CCCAATGGGC 
GACAGCTGCA CCTCCATCGA AACCCAGTAC ACATCCCAGG CCAGCCTGCC AGTGGGCCCA 
CCAAATGCCA CCAGTACGCC GTTCAGTAGC AGCATGTCAT TGCCCATCGC CGGACCCGTA 
AACAATCAAC CGCCGGCCAG CGGACCCTTT CCCACTCTGC CCCGCGAACC CAGCACCTCG 
CGCGGATTCT TTGACTCCAA TGGTGGCACT CTGGCAGATA AGCTGTGGCA CGTCTCACTG 
CAAATCCCGC CGGGAGCCAT TCCAGCCGGA GTACGGCAAG AGATCTACTT CACCGTGAGC 
GATCCGCGAA TGGGACAAGC CGTCGGCGGT CCTCCGCTGG ACATGGAAAA TGGTGAGACA 
ATGCTGTCAC CGCTGGTGAT GTGCGGGCCG CAGGGCCTTG AGTTCCTGGT ACCGGTTACA 
CTCAACATAC CGCACTGCGC CGGACGAACT GCATCACTGG GACTGGCCCT CAAGGCGACC 
GACAGCGAGA AGAACCTGCA CACCGAGTGG GACAACATCG ACCTGCCCAG CAATGCCGCC 
GCCCACACGG TCTCCGTCAA GGTGGATCAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010026.1
CDS	142..7737
Translation	MTREEEEEVRSLLSRHQERIMLDLGSTDLLAVLVKNSVLSQSEEDLLLKPYNAVTPTPSAAASATVEPPGSAPSLTKRKLSAVASSGSTASGGSAGSGGSGSSRCASVNGDANCDSRSLTPSGGGPCSVLNDAEILRVQCSNLIEIIAKNGFEKFKQFCYAIECECPQLIEDLINDRLKTDAANLEDSRDGEKMQKEIETIDYIDKEKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_015154540.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant B (Dere\GG17413), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154540.1

ORF Insert Sequence:

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGCCAGTAGA ATCCCTCTCC 
GACGATTTCC TATTCCCCAT GACCACATCC CGACTCTCGT ATGCATCAAG TCCCGAATCC 
GATTTGGAAC TGAGTCCCGG CCCATCCGCA TCATTGTCGC TTGGCAATTT GCCGCAGTTG 
GTTAAAGCGA GCTCAGATCC ATCGATTGCC ACTAATCAGG ATAACTTGGA TAGGGATAGA 
GACATAATTG GTGAAGGACT ACCACCTCCA TATACGGTAC CCTACGACCA TGGTGTCCCC 
GCGAATCCCA ATCGCCGCCA GACCATGGAC TCCAGCAAGT ACAGCATCTA CGGCACCAAC 
GTGCCGCCGC AGCCGCAGCA GCCCGGCGCA GGAGGAGATC CGGCTGCAGT GAGGCCACAG 
TCTCTGTACG GGATTAATGC ACCCGATCTG CCGCCCCGCA TCGATCGTCA GTCCAAGCCG 
GGTGATATGC CACTGAACAC CTCGGGCTCG TCGTCGCGAA ATGGCACCCT GGGTCGCAGT 
GCCCAGGAGC GTCTGTTTGG CAAGGCTGTG GTGCAGGACG ATGTCCAGGC GGAATACATA 
ACCCGAAATG CTCTGGTCGG CTCCGGGGCG GCTGAAACCC TGGATCGCCA GCAGCAGCAG 
CAAACCCACG CGTCCTTGGA GCGGCAGGCG CGTTTGAATG CGCAGCTGAA GGCCAACGGA 
GGAGGCGCCG GAGGAGGTGC ATCCACCTAC GACAGTGTGT CCAGCTACGA CTCGTACAAC 
AACACACAAA TGGCCATGCA AAACCTGGGC CGACTGGGTC CCAATGCACC CGACGATCTC 
AAATCAGTGC CAAATGCAAG TGGTCGCCCC TTGCCTCCGA CGGGTCAGTC GCTCGACTAC 
GGACGCACTC CGCACGATCA CCGCAGCTTC GGAGGAGCCA ACGATCTGAA CCGACAGAGC 
AGTCCGGGCA GACCGCACTA CCATGACATG AATGCGTCCC GTAATATTGA TCCACGCAAT 
GGCACTCCAC AGCGTCCTTC GAATCTGGGC CTAGAAAGCA GTCCACGCAA GCCTTTGGTG 
GAGACGAAGA CGGATTACGG CAAATACAGT CGAAACAACT CCGTATCGCA AGCGGATTAC 
ACCAAGCTAC CGAAGACGGC GCCACATGGA GTGGTACCGC CGCCGAATGT GAGCAACGGC 
CAAAGCCAGA TGAATGGCAG TGGAACGCCC AGCAGCAATG GCAGTGGACC CTTCAAGCCG 
GTGCCGCCAC CCAAACCCAA GAACTATAGG CCACCGGTGC AAAGTGGTGG CAGCAGTGGC 
AGCGGTGGCA CTACTCCGTG GGAAAATGGG GACTCTGGCT CGCCACGCTC TCCAAATGGC 
TTCTATTATC CACCAACGCC TTCGCATCAT CACTACGGCC AGCAGGCGAC TCCCGGTTCA 
CCCAGCAACG GCCACATGCA GCCACCTCCG CCGCAGCAGC AGCAGCAGCA GCCCACCTAC 
GGCGGAAGTA ATGGCAACTA CGGACAGGCG CCACCACCAC AACCCTATCC GCAGGCCAAT 
GGCTACAACG GGAATGGCCA TCACTACAAC GGAGGCAGCG GCACGGGACC CTACATTGCA 
CCACATCGCG GCATGCCGCC GCCAATAGGA AACCTGCCGC CCCACACGCC CGAACGTCAT 
GCCCTGGACT TGGCCGGAAG TCGGGAGCAG CGTGGCTCGG CCTTCGAGCT GTATCGTAAG 
CCGCAAATCG GAGCAACCGC TGGGCACCAT CACAACATGA GCGAAATGGA GCCGTACGAC 
GAGCGATACG ATGATTACTA TAACATGCCA CCCCCTGCCG CCCATCCATC CCAAGGGCAC 
CATATGCAGC GCTCCCGATC AGCTCCTCGT TATCCCCAGG AGCGACCTCC TCACGCACAA 
GATCCGAATT ACTACGGCCA CTATGGAACT TCTAGGGGAC ATAGTCAGCC GCGCCAGCAG 
TATCCTCAGC ATCACCAGCA ACAGTATTAC GATGAGCATG GCATGGAGAT GGGTCCACCG 
CCACTGCCCC CACATAAGAA GAAGAAATCG GTGCTGAAGA GCCCACTGGT GGCCCTCAAG 
AATGCGCTGC TTAAGAGCAC GCGGCCCCTG AGGAGAATGA ACAGCATGGT GGAGCCGGAG 
AGGAAACCGA AGGGACTCCG ACGCCAGCAA TCGATGCTGG AGAGGGGTGT GCAAAGACCC 
TACTATCCGG ATGAGTATCC AACCTATCCA GCTGGCTTTG AGGAGCGTGC CCATGGCGGA 
CAAGGCATGA TGCAGCCTCA TCCGCAAGAT GCCTACTATC AAAGGGGTGG TCACTACACG 
TCGCAGCAGC AGGAGATGAT GAACAGCACC TACCAGAATC TGGAGGGTGA GGATATCTAT 
GGGAACATCG GAAACACAGT TCCTAGAATG CCGCATCACC AGGACAATGG TTACGGATAT 
GACCAATACG ATCTCTATGC CAATCGAGCG TGCATCGATC TCGAAAGAAG GCAAGCAGAG 
GCAGCTGCAG CTTCCGGTGG CAGAAATGGG AGGAGAATTG TGAGGCGGCA CAGCACCACC 
ACTGCGGATC GAGGAGGAAA TCCCGGACCG CCCAGGAGGC CTATGATCAG TCCTGGTTAT 
GAGCAGGATC CGCAAGAGAT CTACCAGACC CGCAACGGAG CCTACATGCT GCCCGATCAG 
CGAAGGGCGC CCAGTTCGGA GGTGATGACC CGCAGAAGAT TCTATCCGGG GGCTGCCAAT 
GAGCAAACTG AGGAGGAGCC CCTCTACCAA TCCCGTCGCG AAATGCAGCG TGAGATGCAG 
AGGAATCATC TGTACCAATC GAAACGGGAA ATGCAGGAGA GGATTAGCCA GGGCAAGAGG 
GATATGGAGC GAGAGTTTAG CCCCCAGGGC AGCAGCAGCC AGTCGGAGAC CTCCAATCCG 
GAGGTAATAT ACCAGAGTCG CCGGGAGGCC AAGGAAAGTG CTCTGAAAAC GAGGGCACAA 
CTGAGGGATC AGATCTATCA GACTAGACGG GAAGCCCTGG ACAGCATGGC GGAACCGATC 
TATGTGTCCA AAAGAGATAT GGGCAGACCG GCTCCTATCT ATGAGACTCG GGAAGAGAGT 
ATCCTCCAGT CCCGAGAAAA TGAAACCGAC GAAAAGAAGG AAAAGGAGTG CAAGACCGAG 
CAGATTCAGG TGGAGATTAA TGCTCAACCG GAGGAACAGG TGGAAGATTC GACCTTAAGT 
CGATCCGACT TGCAGAAATC TTCGGACACG GTGATAGAAA ATCCAGCGAG AGCTCCTCTT 
GTGCAGGATG AGGTAGATGA CGACGAAGAA GAGCAGGCTC AGGCTCGGGA TGATCCCGCT 
GAAAGCTCTG CGGATCAGAC AGAGCTGCCA ACGGCCATTG AAAACAATCT ACACAACGAG 
GGAATGGTGG AGCAAACACA GAATGAGTCG GATGACGTAT TCGAAGCAGC CGATAAGGTT 
TCTCCTCTAG CCGCTCCTAC AGGTACTGTT CAACCGCCGA CCCCGCGATC CAGTAGAGCT 
CCCTTCCACA TTTCGAATAT CCTAAAGCGA ACAGCACCTC CTCCGAGTTC CCCAATGGGC 
GACAGCTGCA CCTCCATCGA AACCCAGTAC ACATCCCAGG CCAGCCTGCC AGTGGGCCCA 
CCAAATGCCA CCAGTACGCC GTTCAGTAGC AGCATGTCAT TGCCCATCGC CGGACCCGTA 
AACAATCAAC CGCCGGCCAG CGGACCCTTT CCCACTCTGC CCCGCGAACC CAGCACCTCG 
CGCGGATTCT TTGACTCCAA TGGTGGCACT CTGGCAGATA AGCTGTGGCA CGTCTCACTG 
CAAATCCCGC CGGGAGCCAT TCCAGCCGGA GTACGGCAAG AGATCTACTT CACCGTGAGC 
GATCCGCGAA TGGGACAAGC CGTCGGCGGT CCTCCGCTGG ACATGGAAAA TGGTGAGACA 
ATGCTGTCAC CGCTGGTGAT GTGCGGGCCG CAGGGCCTTG AGTTCCTGGT ACCGGTTACA 
CTCAACATAC CGCACTGCGC CGGACGAACT GCATCACTGG GACTGGCCCT CAAGGCGACC 
GACAGCGAGA AGAACCTGCA CACCGAGTGG GACAACATCG ACCTGCCCAG CAATGCCGCC 
GCCCACACGG TCTCCGTCAA GGTGGATCAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154541.1 ) cDNA ORF clone, Drosophila erecta -> XP_015010027.1 Drosophila erecta uncharacterized protein, transcript variant C (Dere\GG17413), mRNA.

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CloneID	ODi33928
Clone ID Related Accession (Same CDS sequence)	XM_015154541.1
Accession Version	XM_015154541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956552). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATAG TCAGCGGATC GGCATCGGGC AACCAGCTCG TCCTGCCAAA CAATCATTTT 
CGCCATGAAT CATTTCAGCA ACAGGGTGAT CGAACCACTT GGGAGTACCA CACGGTGGCA 
GTGACTCGGG TGCCGGGCTA TGGATTCGGC ATCGCCGTTT CCGGTGGACG TGATAATCCG 
CACTTCGCCA ACGGCGATCC CTCGATAGCA GTTAGCGATG TGCTGAAAGG TGGCCCCGCC 
GAGGATCGAT TGCAAGTCAA CGATCGGATA ATCTCGGTGA ACGGCGTCTC CCTGGAGAAC 
GTGGAATATG CCACCGCGGT GCAGGTGTTG CGCGATAGTG GCAACACGGT CCAATTGGTG 
GTCAAGCGTC GCGTGCCCCT CAATCCCATC AATAATGGAG GTGCAGTGCA GCATCAGCAC 
TCGCACAGTC TCAGCTCGGT TGGTCTATTG GCCAATGGAA GTGGAGGAGT TGCACCAACG 
CCCTTGACGA GCCTGAGTCA ACCCAACTCC CTGAACTCTT CGCTGGTGCA GAATGCCAGC 
AGTGGTCAGC CCATCAAGGT GACCCTCACG AAAGGCGGCA AGAAAGATGA CTATGGCGTG 
GTCCTTGGCT GCCGGCTGTT TGTTAAGGAG ATCTCCTCCA AAGCTCGCGA GCAACTTAAT 
GCCAATGGCT ACAGTTTGCA AGAGGGTGAC ATTATCACCC GCATCCACAA CACCAATTGC 
GGGGATACGA TGAGTCTCAA GGAGGCGAAA AAGATCATCG ATGGTTGCAA GGAGCGCTTG 
AACCTAGTGG TTCTACGGGA CATCACCAAC CAAGCAGCTG TTAGCCAACT GAATCTGAAC 
AATTCAGCGA GCCACCAGGC TTCGGCAAAT ATATACGGCA CTCATCAGCC TCAGGTATCC 
GGATGTAGCA GCAGTAACAA CAATCTTGAA GATCCATATC TGCCGGGCGG AGCCAGTTAC 
TCCTCGCAGA ATCTGTACGT GCAACCACCC ACTCGCACCT CCAATGGTCC CAATCTCAAT 
GGCAATGGGC TGAACGACGA AAAGAGTAAT CTCACACCGC GAGGTCGCTC CCGGGGGCCC 
ATTATGGATG GGGTATCTCT GCAGCAGCTG GACAGGCCCG TAACACCGAC GCGTGGCAGG 
AGTGCGGCCA TCGACGAGCC GCCACGTCCA CCGCCGCCAC GAGGATCGAG CGGGGGAGCG 
GCCCAGGAGG ATTTCTACAG CTCTCGCAGG CAATTGTACG AGGAGCGCCA GAGTGCCGAG 
CCGAGATTCA TTTCCTTCCA GAAGGAGGGA AGCGTGGGCA TCCGCCTAAC CGGCGGCAAT 
GAAGCCGGTA TTTTTGTGAC TGCCGTACAA CCGGGATCTC CAGCCTCGCT GCAGGGTCTA 
ATGCCAGGCG ATAAGATCCT GAAGGTCAAC GACATGGACA TGAACGGAGT GACGCGCGAG 
GAGGCTGTTC TCTTTCTGTT GAGCCTGCAG GATCGCATCG ATCTGATTGT GCAGTACTGC 
AAGGAGGAGT ACGACGAGGT GGTGACCAAC CAACGTGGCG ACTCCTTCCA CATCAAGACA 
CATTTCCACT GCGATAATCC CTCCAAAGGC GAGATGGCCT TCAAGGCGGG CGATGTGTTC 
CGGGTGATCG ATACCCTGCA CAATGGTGTA GTCGGTTCCT GGCAGGTGCT GAAGATCGGA 
CGTGGTCACC AGGAGATGCA GCGCGGCGTT ATACCGAACA AGTCGCGGGC CGAAGAACTG 
GCCACTGCCC AGTTCAATGC CACCAAGAAG GAGATGAACG CCAACGAGTC GAGGGGCAAC 
TTCTTCCGGC GACGACGCTC GACGCACCGA CGCTCGAAGA GTCTGTCCCG CGAGAACTGG 
GACGACGTGG TCTTCTCCGA CAGCATTTCC AAATTCCCCG CCTACGAGCG AGTCGTTCTC 
CGCCATCCTG GCTTTGTTCG ACCCGTAGTG CTCTTCGGAC CCGTATCCGA TCTGGCCCGC 
GAGCGCTTGG CCAAGGATTT CCCCGACAAG TTTTCGACTC CACTGCAGGA TGATGACAAG 
TCAGCTGCAA CCAGTGGAAA GTGCCGCATC GTAAGGCTCT CGAACATACG CGATGTGATG 
GATCGTGGCA AGCACGCCCT GCTGGACATA ACGCCCAATG CAGTGGATCG TCTCAACTAC 
GCCCAGTTCT ATCCGGTGGT GATCTTCCTG AAGACAGACA GCAAGCACGT GATCAAGCAG 
CTGCGCCACG GACTACCCAA GGCGGCCCAC AAGAGCTCCA AGAAGCTGCT GGAGCAGACC 
CAGAAACTGG AGCGCGTCTG GTCCCACATC TTTAGCACCC AGATCGCGTT GAGCGACGAG 
GAGTCCTGGT ACCGAAAGTT GCGCGACTCG ATCGATCTGC AGCAGAGCGG CGCCGTGTGG 
ATGTCCGAGT CCAAGCCAGT AGAATCCCTC TCCGACGATT TCCTATTCCC CATGACCACA 
TCCCGACTCT CGTATGCATC AAGTCCCGAA TCCGATTTGG AACTGAGTCC CGGCCCATCC 
GCATCATTGT CGCTTGGCAA TTTGCCGCAG TTGGTTAAAG CGAGCTCAGA TCCATCGATT 
GCCACTAATC AGGATAACTT GGATAGGGAT AGAGACATAA TTGGTGAAGG ACTACCACCT 
CCATATACGG TACCCTACGA CCATGGTGTC CCCGCGAATC CCAATCGCCG CCAGACCATG 
GACTCCAGCA AGTACAGCAT CTACGGCACC AACGTGCCGC CGCAGCCGCA GCAGCCCGGC 
GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA CAGTCTCTGT ACGGGATTAA TGCACCCGAT 
CTGCCGCCCC GCATCGATCG TCAGTCCAAG CCGGGTGATA TGCCACTGAA CACCTCGGGC 
TCGTCGTCGC GAAATGGCAC CCTGGGTCGC AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT 
GTGGTGCAGG ACGATGTCCA GGCGGAATAC ATAACCCGAA ATGCTCTGGT CGGCTCCGGG 
GCGGCTGAAA CCCTGGATCG CCAGCAGCAG CAGCAAACCC ACGCGTCCTT GGAGCGGCAG 
GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC GGAGGAGGCG CCGGAGGAGG TGCATCCACC 
TACGACAGTG TGTCCAGCTA CGACTCGTAC AACAACACAC AAATGGCCAT GCAAAACCTG 
GGCCGACTGG GTCCCAATGC ACCCGACGAT CTCAAATCAG TGCCAAATGC AAGTGGTCGC 
CCCTTGCCTC CGACGGGTCA GTCGCTCGAC TACGGACGCA CTCCGCACGA TCACCGCAGC 
TTCGGAGGAG CCAACGATCT GAACCGACAG AGCAGTCCGG GCAGACCGCA CTACCATGAC 
ATGAATGCGT CCCGTAATAT TGATCCACGC AATGGCACTC CACAGCGTCC TTCGAATCTG 
GGCCTAGAAA GCAGTCCACG CAAGCCTTTG GTGGAGACGA AGACGGATTA CGGCAAATAC 
AGTCGAAACA ACTCCGTATC GCAAGCGGAT TACACCAAGC TACCGAAGAC GGCGCCACAT 
GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC GGCCAAAGCC AGATGAATGG CAGTGGAACG 
CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG CCGGTGCCGC CACCCAAACC CAAGAACTAT 
AGGCCACCGG TGCAAAGTGG TGGCAGCAGT GGCAGCGGTG GCACTACTCC GTGGGAAAAT 
GGGGACTCTG GCTCGCCACG CTCTCCAAAT GGCTTCTATT ATCCACCAAC GCCTTCGCAT 
CATCACTACG GCCAGCAGGC GACTCCCGGT TCACCCAGCA ACGGCCACAT GCAGCCACCT 
CCGCCGCAGC AGCAGCAGCA GCAGCCCACC TACGGCGGAA GTAATGGCAA CTACGGACAG 
GCGCCACCAC CACAACCCTA TCCGCAGGCC AATGGCTACA ACGGGAATGG CCATCACTAC 
AACGGAGGCA GCGGCACGGG ACCCTACATT GCACCACATC GCGGCATGCC GCCGCCAATA 
GGAAACCTGC CGCCCCACAC GCCCGAACGT CATGCCCTGG ACTTGGCCGG AAGTCGGGAG 
CAGCGTGGCT CGGCCTTCGA GCTGTATCGT AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC 
CATCACAACA TGAGCGAAAT GGAGCCGTAC GACGAGCGAT ACGATGATTA CTATAACATG 
CCACCCCCTG CCGCCCATCC ATCCCAAGGG CACCATATGC AGCGCTCCCG ATCAGCTCCT 
CGTTATCCCC AGGAGCGACC TCCTCACGCA CAAGATCCGA ATTACTACGG CCACTATGGA 
ACTTCTAGGG GACATAGTCA GCCGCGCCAG CAGTATCCTC AGCATCACCA GCAACAGTAT 
TACGATGAGC ATGGCATGGA GATGGGTCCA CCGCCACTGC CCCCACATAA GAAGAAGAAA 
TCGGTGCTGA AGAGCCCACT GGTGGCCCTC AAGAATGCGC TGCTTAAGAG CACGCGGCCC 
CTGAGGAGAA TGAACAGCAT GGTGGAGCCG GAGAGGAAAC CGAAGGGACT CCGACGCCAG 
CAATCGATGC TGGAGAGGGG TGTGCAAAGA CCCTACTATC CGGATGAGTA TCCAACCTAT 
CCAGCTGGCT TTGAGGAGCG TGCCCATGGC GGACAAGGCA TGATGCAGCC TCATCCGCAA 
GATGCCTACT ATCAAAGGGG TGGTCACTAC ACGTCGCAGC AGCAGGAGAT GATGAACAGC 
ACCTACCAGA ATCTGGAGGG TGAGGATATC TATGGGAACA TCGGAAACAC AGTTCCTAGA 
ATGCCGCATC ACCAGGACAA TGGTTACGGA TATGACCAAT ACGATCTCTA TGCCAATCGA 
GCGTGCATCG ATCTCGAAAG AAGGCAAGCA GAGGCAGCTG CAGCTTCCGG TGGCAGAAAT 
GGGAGGAGAA TTGTGAGGCG GCACAGCACC ACCACTGCGG ATCGAGGAGG AAATCCCGGA 
CCGCCCAGGA GGCCTATGAT CAGTCCTGGT TATGAGCAGG ATCCGCAAGA GATCTACCAG 
ACCCGCAACG GAGCCTACAT GCTGCCCGAT CAGCGAAGGG CGCCCAGTTC GGAGGTGATG 
ACCCGCAGAA GATTCTATCC GGGGGCTGCC AATGAGCAAA CTGAGGAGGA GCCCCTCTAC 
CAATCCCGTC GCGAAATGCA GCGTGAGATG CAGAGGAATC ATCTGTACCA ATCGAAACGG 
GAAATGCAGG AGAGGATTAG CCAGGGCAAG AGGGATATGG AGCGAGAGTT TAGCCCCCAG 
GGCAGCAGCA GCCAGTCGGA GACCTCCAAT CCGGAGGTAA TATACCAGAG TCGCCGGGAG 
GCCAAGGAAA GTGCTCTGAA AACGAGGGCA CAACTGAGGG ATCAGATCTA TCAGACTAGA 
CGGGAAGCCC TGGACAGCAT GGCGGAACCG ATCTATGTGT CCAAAAGAGA TATGGGCAGA 
CCGGCTCCTA TCTATGAGAC TCGGGAAGAG AGTATCCTCC AGTCCCGAGA AAATGAAACC 
GACGAAAAGA AGGAAAAGGA GTGCAAGACC GAGCAGATTC AGGTGGAGAT TAATGCTCAA 
CCGGAGGAAC AGGTGGAAGA TTCGACCTTA AGTCGATCCG ACTTGCAGAA ATCTTCGGAC 
ACGGTGATAG AAAATCCAGC GAGAGCTCCT CTTGTGCAGG ATGAGGTAGA TGACGACGAA 
GAAGAGCAGG CTCAGGCTCG GGATGATCCC GCTGAAAGCT CTGCGGATCA GACAGAGCTG 
CCAACGGCCA TTGAAAACAA TCTACACAAC GAGGGAATGG TGGAGCAAAC ACAGAATGAG 
TCGGATGACG TATTCGAAGC AGCCGATAAG GTTTCTCCTC TAGCCGCTCC TACAGGTACT 
GTTCAACCGC CGACCCCGCG ATCCAGTAGA GCTCCCTTCC ACATTTCGAA TATCCTAAAG 
CGAACAGCAC CTCCTCCGAG TTCCCCAATG GGCGACAGCT GCACCTCCAT CGAAACCCAG 
TACACATCCC AGGCCAGCCT GCCAGTGGGC CCACCAAATG CCACCAGTAC GCCGTTCAGT 
AGCAGCATGT CATTGCCCAT CGCCGGACCC GTAAACAATC AACCGCCGGC CAGCGGACCC 
TTTCCCACTC TGCCCCGCGA ACCCAGCACC TCGCGCGGAT TCTTTGACTC CAATGGTGGC 
ACTCTGGCAG ATAAGCTGTG GCACGTCTCA CTGCAAATCC CGCCGGGAGC CATTCCAGCC 
GGAGTACGGC AAGAGATCTA CTTCACCGTG AGCGATCCGC GAATGGGACA AGCCGTCGGC 
GGTCCTCCGC TGGACATGGA AAATGGTGAG ACAATGCTGT CACCGCTGGT GATGTGCGGG 
CCGCAGGGCC TTGAGTTCCT GGTACCGGTT ACACTCAACA TACCGCACTG CGCCGGACGA 
ACTGCATCAC TGGGACTGGC CCTCAAGGCG ACCGACAGCG AGAAGAACCT GCACACCGAG 
TGGGACAACA TCGACCTGCC CAGCAATGCC GCCGCCCACA CGGTCTCCGT CAAGGTGGAT 
CACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010027.1
CDS	407..6955
Translation	MDIVSGSASGNQLVLPNNHFRHESFQQQGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_015154541.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant C (Dere\GG17413), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154541.1

ORF Insert Sequence:

ATGGATATAG TCAGCGGATC GGCATCGGGC AACCAGCTCG TCCTGCCAAA CAATCATTTT 
CGCCATGAAT CATTTCAGCA ACAGGGTGAT CGAACCACTT GGGAGTACCA CACGGTGGCA 
GTGACTCGGG TGCCGGGCTA TGGATTCGGC ATCGCCGTTT CCGGTGGACG TGATAATCCG 
CACTTCGCCA ACGGCGATCC CTCGATAGCA GTTAGCGATG TGCTGAAAGG TGGCCCCGCC 
GAGGATCGAT TGCAAGTCAA CGATCGGATA ATCTCGGTGA ACGGCGTCTC CCTGGAGAAC 
GTGGAATATG CCACCGCGGT GCAGGTGTTG CGCGATAGTG GCAACACGGT CCAATTGGTG 
GTCAAGCGTC GCGTGCCCCT CAATCCCATC AATAATGGAG GTGCAGTGCA GCATCAGCAC 
TCGCACAGTC TCAGCTCGGT TGGTCTATTG GCCAATGGAA GTGGAGGAGT TGCACCAACG 
CCCTTGACGA GCCTGAGTCA ACCCAACTCC CTGAACTCTT CGCTGGTGCA GAATGCCAGC 
AGTGGTCAGC CCATCAAGGT GACCCTCACG AAAGGCGGCA AGAAAGATGA CTATGGCGTG 
GTCCTTGGCT GCCGGCTGTT TGTTAAGGAG ATCTCCTCCA AAGCTCGCGA GCAACTTAAT 
GCCAATGGCT ACAGTTTGCA AGAGGGTGAC ATTATCACCC GCATCCACAA CACCAATTGC 
GGGGATACGA TGAGTCTCAA GGAGGCGAAA AAGATCATCG ATGGTTGCAA GGAGCGCTTG 
AACCTAGTGG TTCTACGGGA CATCACCAAC CAAGCAGCTG TTAGCCAACT GAATCTGAAC 
AATTCAGCGA GCCACCAGGC TTCGGCAAAT ATATACGGCA CTCATCAGCC TCAGGTATCC 
GGATGTAGCA GCAGTAACAA CAATCTTGAA GATCCATATC TGCCGGGCGG AGCCAGTTAC 
TCCTCGCAGA ATCTGTACGT GCAACCACCC ACTCGCACCT CCAATGGTCC CAATCTCAAT 
GGCAATGGGC TGAACGACGA AAAGAGTAAT CTCACACCGC GAGGTCGCTC CCGGGGGCCC 
ATTATGGATG GGGTATCTCT GCAGCAGCTG GACAGGCCCG TAACACCGAC GCGTGGCAGG 
AGTGCGGCCA TCGACGAGCC GCCACGTCCA CCGCCGCCAC GAGGATCGAG CGGGGGAGCG 
GCCCAGGAGG ATTTCTACAG CTCTCGCAGG CAATTGTACG AGGAGCGCCA GAGTGCCGAG 
CCGAGATTCA TTTCCTTCCA GAAGGAGGGA AGCGTGGGCA TCCGCCTAAC CGGCGGCAAT 
GAAGCCGGTA TTTTTGTGAC TGCCGTACAA CCGGGATCTC CAGCCTCGCT GCAGGGTCTA 
ATGCCAGGCG ATAAGATCCT GAAGGTCAAC GACATGGACA TGAACGGAGT GACGCGCGAG 
GAGGCTGTTC TCTTTCTGTT GAGCCTGCAG GATCGCATCG ATCTGATTGT GCAGTACTGC 
AAGGAGGAGT ACGACGAGGT GGTGACCAAC CAACGTGGCG ACTCCTTCCA CATCAAGACA 
CATTTCCACT GCGATAATCC CTCCAAAGGC GAGATGGCCT TCAAGGCGGG CGATGTGTTC 
CGGGTGATCG ATACCCTGCA CAATGGTGTA GTCGGTTCCT GGCAGGTGCT GAAGATCGGA 
CGTGGTCACC AGGAGATGCA GCGCGGCGTT ATACCGAACA AGTCGCGGGC CGAAGAACTG 
GCCACTGCCC AGTTCAATGC CACCAAGAAG GAGATGAACG CCAACGAGTC GAGGGGCAAC 
TTCTTCCGGC GACGACGCTC GACGCACCGA CGCTCGAAGA GTCTGTCCCG CGAGAACTGG 
GACGACGTGG TCTTCTCCGA CAGCATTTCC AAATTCCCCG CCTACGAGCG AGTCGTTCTC 
CGCCATCCTG GCTTTGTTCG ACCCGTAGTG CTCTTCGGAC CCGTATCCGA TCTGGCCCGC 
GAGCGCTTGG CCAAGGATTT CCCCGACAAG TTTTCGACTC CACTGCAGGA TGATGACAAG 
TCAGCTGCAA CCAGTGGAAA GTGCCGCATC GTAAGGCTCT CGAACATACG CGATGTGATG 
GATCGTGGCA AGCACGCCCT GCTGGACATA ACGCCCAATG CAGTGGATCG TCTCAACTAC 
GCCCAGTTCT ATCCGGTGGT GATCTTCCTG AAGACAGACA GCAAGCACGT GATCAAGCAG 
CTGCGCCACG GACTACCCAA GGCGGCCCAC AAGAGCTCCA AGAAGCTGCT GGAGCAGACC 
CAGAAACTGG AGCGCGTCTG GTCCCACATC TTTAGCACCC AGATCGCGTT GAGCGACGAG 
GAGTCCTGGT ACCGAAAGTT GCGCGACTCG ATCGATCTGC AGCAGAGCGG CGCCGTGTGG 
ATGTCCGAGT CCAAGCCAGT AGAATCCCTC TCCGACGATT TCCTATTCCC CATGACCACA 
TCCCGACTCT CGTATGCATC AAGTCCCGAA TCCGATTTGG AACTGAGTCC CGGCCCATCC 
GCATCATTGT CGCTTGGCAA TTTGCCGCAG TTGGTTAAAG CGAGCTCAGA TCCATCGATT 
GCCACTAATC AGGATAACTT GGATAGGGAT AGAGACATAA TTGGTGAAGG ACTACCACCT 
CCATATACGG TACCCTACGA CCATGGTGTC CCCGCGAATC CCAATCGCCG CCAGACCATG 
GACTCCAGCA AGTACAGCAT CTACGGCACC AACGTGCCGC CGCAGCCGCA GCAGCCCGGC 
GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA CAGTCTCTGT ACGGGATTAA TGCACCCGAT 
CTGCCGCCCC GCATCGATCG TCAGTCCAAG CCGGGTGATA TGCCACTGAA CACCTCGGGC 
TCGTCGTCGC GAAATGGCAC CCTGGGTCGC AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT 
GTGGTGCAGG ACGATGTCCA GGCGGAATAC ATAACCCGAA ATGCTCTGGT CGGCTCCGGG 
GCGGCTGAAA CCCTGGATCG CCAGCAGCAG CAGCAAACCC ACGCGTCCTT GGAGCGGCAG 
GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC GGAGGAGGCG CCGGAGGAGG TGCATCCACC 
TACGACAGTG TGTCCAGCTA CGACTCGTAC AACAACACAC AAATGGCCAT GCAAAACCTG 
GGCCGACTGG GTCCCAATGC ACCCGACGAT CTCAAATCAG TGCCAAATGC AAGTGGTCGC 
CCCTTGCCTC CGACGGGTCA GTCGCTCGAC TACGGACGCA CTCCGCACGA TCACCGCAGC 
TTCGGAGGAG CCAACGATCT GAACCGACAG AGCAGTCCGG GCAGACCGCA CTACCATGAC 
ATGAATGCGT CCCGTAATAT TGATCCACGC AATGGCACTC CACAGCGTCC TTCGAATCTG 
GGCCTAGAAA GCAGTCCACG CAAGCCTTTG GTGGAGACGA AGACGGATTA CGGCAAATAC 
AGTCGAAACA ACTCCGTATC GCAAGCGGAT TACACCAAGC TACCGAAGAC GGCGCCACAT 
GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC GGCCAAAGCC AGATGAATGG CAGTGGAACG 
CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG CCGGTGCCGC CACCCAAACC CAAGAACTAT 
AGGCCACCGG TGCAAAGTGG TGGCAGCAGT GGCAGCGGTG GCACTACTCC GTGGGAAAAT 
GGGGACTCTG GCTCGCCACG CTCTCCAAAT GGCTTCTATT ATCCACCAAC GCCTTCGCAT 
CATCACTACG GCCAGCAGGC GACTCCCGGT TCACCCAGCA ACGGCCACAT GCAGCCACCT 
CCGCCGCAGC AGCAGCAGCA GCAGCCCACC TACGGCGGAA GTAATGGCAA CTACGGACAG 
GCGCCACCAC CACAACCCTA TCCGCAGGCC AATGGCTACA ACGGGAATGG CCATCACTAC 
AACGGAGGCA GCGGCACGGG ACCCTACATT GCACCACATC GCGGCATGCC GCCGCCAATA 
GGAAACCTGC CGCCCCACAC GCCCGAACGT CATGCCCTGG ACTTGGCCGG AAGTCGGGAG 
CAGCGTGGCT CGGCCTTCGA GCTGTATCGT AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC 
CATCACAACA TGAGCGAAAT GGAGCCGTAC GACGAGCGAT ACGATGATTA CTATAACATG 
CCACCCCCTG CCGCCCATCC ATCCCAAGGG CACCATATGC AGCGCTCCCG ATCAGCTCCT 
CGTTATCCCC AGGAGCGACC TCCTCACGCA CAAGATCCGA ATTACTACGG CCACTATGGA 
ACTTCTAGGG GACATAGTCA GCCGCGCCAG CAGTATCCTC AGCATCACCA GCAACAGTAT 
TACGATGAGC ATGGCATGGA GATGGGTCCA CCGCCACTGC CCCCACATAA GAAGAAGAAA 
TCGGTGCTGA AGAGCCCACT GGTGGCCCTC AAGAATGCGC TGCTTAAGAG CACGCGGCCC 
CTGAGGAGAA TGAACAGCAT GGTGGAGCCG GAGAGGAAAC CGAAGGGACT CCGACGCCAG 
CAATCGATGC TGGAGAGGGG TGTGCAAAGA CCCTACTATC CGGATGAGTA TCCAACCTAT 
CCAGCTGGCT TTGAGGAGCG TGCCCATGGC GGACAAGGCA TGATGCAGCC TCATCCGCAA 
GATGCCTACT ATCAAAGGGG TGGTCACTAC ACGTCGCAGC AGCAGGAGAT GATGAACAGC 
ACCTACCAGA ATCTGGAGGG TGAGGATATC TATGGGAACA TCGGAAACAC AGTTCCTAGA 
ATGCCGCATC ACCAGGACAA TGGTTACGGA TATGACCAAT ACGATCTCTA TGCCAATCGA 
GCGTGCATCG ATCTCGAAAG AAGGCAAGCA GAGGCAGCTG CAGCTTCCGG TGGCAGAAAT 
GGGAGGAGAA TTGTGAGGCG GCACAGCACC ACCACTGCGG ATCGAGGAGG AAATCCCGGA 
CCGCCCAGGA GGCCTATGAT CAGTCCTGGT TATGAGCAGG ATCCGCAAGA GATCTACCAG 
ACCCGCAACG GAGCCTACAT GCTGCCCGAT CAGCGAAGGG CGCCCAGTTC GGAGGTGATG 
ACCCGCAGAA GATTCTATCC GGGGGCTGCC AATGAGCAAA CTGAGGAGGA GCCCCTCTAC 
CAATCCCGTC GCGAAATGCA GCGTGAGATG CAGAGGAATC ATCTGTACCA ATCGAAACGG 
GAAATGCAGG AGAGGATTAG CCAGGGCAAG AGGGATATGG AGCGAGAGTT TAGCCCCCAG 
GGCAGCAGCA GCCAGTCGGA GACCTCCAAT CCGGAGGTAA TATACCAGAG TCGCCGGGAG 
GCCAAGGAAA GTGCTCTGAA AACGAGGGCA CAACTGAGGG ATCAGATCTA TCAGACTAGA 
CGGGAAGCCC TGGACAGCAT GGCGGAACCG ATCTATGTGT CCAAAAGAGA TATGGGCAGA 
CCGGCTCCTA TCTATGAGAC TCGGGAAGAG AGTATCCTCC AGTCCCGAGA AAATGAAACC 
GACGAAAAGA AGGAAAAGGA GTGCAAGACC GAGCAGATTC AGGTGGAGAT TAATGCTCAA 
CCGGAGGAAC AGGTGGAAGA TTCGACCTTA AGTCGATCCG ACTTGCAGAA ATCTTCGGAC 
ACGGTGATAG AAAATCCAGC GAGAGCTCCT CTTGTGCAGG ATGAGGTAGA TGACGACGAA 
GAAGAGCAGG CTCAGGCTCG GGATGATCCC GCTGAAAGCT CTGCGGATCA GACAGAGCTG 
CCAACGGCCA TTGAAAACAA TCTACACAAC GAGGGAATGG TGGAGCAAAC ACAGAATGAG 
TCGGATGACG TATTCGAAGC AGCCGATAAG GTTTCTCCTC TAGCCGCTCC TACAGGTACT 
GTTCAACCGC CGACCCCGCG ATCCAGTAGA GCTCCCTTCC ACATTTCGAA TATCCTAAAG 
CGAACAGCAC CTCCTCCGAG TTCCCCAATG GGCGACAGCT GCACCTCCAT CGAAACCCAG 
TACACATCCC AGGCCAGCCT GCCAGTGGGC CCACCAAATG CCACCAGTAC GCCGTTCAGT 
AGCAGCATGT CATTGCCCAT CGCCGGACCC GTAAACAATC AACCGCCGGC CAGCGGACCC 
TTTCCCACTC TGCCCCGCGA ACCCAGCACC TCGCGCGGAT TCTTTGACTC CAATGGTGGC 
ACTCTGGCAG ATAAGCTGTG GCACGTCTCA CTGCAAATCC CGCCGGGAGC CATTCCAGCC 
GGAGTACGGC AAGAGATCTA CTTCACCGTG AGCGATCCGC GAATGGGACA AGCCGTCGGC 
GGTCCTCCGC TGGACATGGA AAATGGTGAG ACAATGCTGT CACCGCTGGT GATGTGCGGG 
CCGCAGGGCC TTGAGTTCCT GGTACCGGTT ACACTCAACA TACCGCACTG CGCCGGACGA 
ACTGCATCAC TGGGACTGGC CCTCAAGGCG ACCGACAGCG AGAAGAACCT GCACACCGAG 
TGGGACAACA TCGACCTGCC CAGCAATGCC GCCGCCCACA CGGTCTCCGT CAAGGTGGAT 
CACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154542.1 ) cDNA ORF clone, Drosophila erecta -> XP_015010028.1 Drosophila erecta uncharacterized protein, transcript variant D (Dere\GG17413), mRNA.

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CloneID	ODi33929
Clone ID Related Accession (Same CDS sequence)	XM_015154542.1
Accession Version	XM_015154542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956552). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCCCA GCAGCACGCG ACGTCGCAGT TTCAAGACGA AACCCCCCAA ATCGAATGTT 
TATAACCCGC TGGTTCGCTA TCGCCCTACG GCCTATGTCC GGAACGGAAT TATTGGCGGC 
GGCGAGGGTC CCGGCTCGAG TGGTGTGTAC CAAATCAACG ATAGGGTGTA TACCACCAGC 
AAGATGTTTC TGCGCGACGG CTATATGTAC CCGCGTCAAC CGGGTCAAAC CATGGATCCC 
CGTTTCCCCC TCGAGTTGCA CGAGTTCCCT CAGCAATTCG GCGCCACTGG CCTGCAGTTG 
GTTAGTGCAC CCAAGGTTCA TATAGCCGGC GAATATCTGG CCAATGAACA GCAGCAGCAA 
CAACAGCAGC AGCAGCATCA ACAGGTGCAG GAGCAACAAC AATTGGGCAG AAGAAAGGCT 
GCAGCAGCCG CGGAAATAAA TTTGGCAAAT CAGGTTTACT GGCTGGGCAA GCAAAAGACC 
AGATCCCGAT CCCGGAGCAA TAGTGTGGGC AATCGGAGCA TAACCAGTTG CATGGGCGGC 
GGTGGAGGGG GTGGCGATAA GGAAAGGGAA CGCGTCGGTG ATCGGGATCG TCCCTATATA 
CGGAACGTTG ACGATCTTCT CCAGGCACTG GGCAAAAGGG ATGGGGATAA CACCAGCAAC 
CTGCTCGAAA CTGCTTCGAC GCACTCTTCA TCGCAGCGAA GGCGCTTCAA GAAGGGTGAT 
CGAACCACTT GGGAGTACCA CACGGTGGCA GTGACTCGGG TGCCGGGCTA TGGATTCGGC 
ATCGCCGTTT CCGGTGGACG TGATAATCCG CACTTCGCCA ACGGCGATCC CTCGATAGCA 
GTTAGCGATG TGCTGAAAGG TGGCCCCGCC GAGGATCGAT TGCAAGTCAA CGATCGGATA 
ATCTCGGTGA ACGGCGTCTC CCTGGAGAAC GTGGAATATG CCACCGCGGT GCAGGTGTTG 
CGCGATAGTG GCAACACGGT CCAATTGGTG GTCAAGCGTC GCGTGCCCCT CAATCCCATC 
AATAATGGAG GTGCAGTGCA GCATCAGCAC TCGCACAGTC TCAGCTCGGT TGGTCTATTG 
GCCAATGGAA GTGGAGGAGT TGCACCAACG CCCTTGACGA GCCTGAGTCA ACCCAACTCC 
CTGAACTCTT CGCTGGTGCA GAATGCCAGC AGTGGTCAGC CCATCAAGGT GACCCTCACG 
AAAGGCGGCA AGAAAGATGA CTATGGCGTG GTCCTTGGCT GCCGGCTGTT TGTTAAGGAG 
ATCTCCTCCA AAGCTCGCGA GCAACTTAAT GCCAATGGCT ACAGTTTGCA AGAGGGTGAC 
ATTATCACCC GCATCCACAA CACCAATTGC GGGGATACGA TGAGTCTCAA GGAGGCGAAA 
AAGATCATCG ATGGTTGCAA GGAGCGCTTG AACCTAGTGG TTCTACGGGA CATCACCAAC 
CAAGCAGCTG TTAGCCAACT GAATCTGAAC AATTCAGCGA GCCACCAGGC TTCGGCAAAT 
ATATACGGCA CTCATCAGCC TCAGGTATCC GGATGTAGCA GCAGTAACAA CAATCTTGAA 
GATCCATATC TGCCGGGCGG AGCCAGTTAC TCCTCGCAGA ATCTGTACGT GCAACCACCC 
ACTCGCACCT CCAATGGTCC CAATCTCAAT GGCAATGGGC TGAACGACGA AAAGAGTAAT 
CTCACACCGC GAGGTCGCTC CCGGGGGCCC ATTATGGATG GGGTATCTCT GCAGCAGCTG 
GACAGGCCCG TAACACCGAC GCGTGGCAGG AGTGCGGCCA TCGACGAGCC GCCACGTCCA 
CCGCCGCCAC GAGGATCGAG CGGGGGAGCG GCCCAGGAGG ATTTCTACAG CTCTCGCAGG 
CAATTGTACG AGGAGCGCCA GAGTGCCGAG CCGAGATTCA TTTCCTTCCA GAAGGAGGGA 
AGCGTGGGCA TCCGCCTAAC CGGCGGCAAT GAAGCCGGTA TTTTTGTGAC TGCCGTACAA 
CCGGGATCTC CAGCCTCGCT GCAGGGTCTA ATGCCAGGCG ATAAGATCCT GAAGGTCAAC 
GACATGGACA TGAACGGAGT GACGCGCGAG GAGGCTGTTC TCTTTCTGTT GAGCCTGCAG 
GATCGCATCG ATCTGATTGT GCAGTACTGC AAGGAGGAGT ACGACGAGGT GGTGACCAAC 
CAACGTGGCG ACTCCTTCCA CATCAAGACA CATTTCCACT GCGATAATCC CTCCAAAGGC 
GAGATGGCCT TCAAGGCGGG CGATGTGTTC CGGGTGATCG ATACCCTGCA CAATGGTGTA 
GTCGGTTCCT GGCAGGTGCT GAAGATCGGA CGTGGTCACC AGGAGATGCA GCGCGGCGTT 
ATACCGAACA AGTCGCGGGC CGAAGAACTG GCCACTGCCC AGTTCAATGC CACCAAGAAG 
GAGATGAACG CCAACGAGTC GAGGGGCAAC TTCTTCCGGC GACGACGCTC GACGCACCGA 
CGCTCGAAGA GTCTGTCCCG CGAGAACTGG GACGACGTGG TCTTCTCCGA CAGCATTTCC 
AAATTCCCCG CCTACGAGCG AGTCGTTCTC CGCCATCCTG GCTTTGTTCG ACCCGTAGTG 
CTCTTCGGAC CCGTATCCGA TCTGGCCCGC GAGCGCTTGG CCAAGGATTT CCCCGACAAG 
TTTTCGACTC CACTGCAGGA TGATGACAAG TCAGCTGCAA CCAGTGGAAA GTGCCGCATC 
GTAAGGCTCT CGAACATACG CGATGTGATG GATCGTGGCA AGCACGCCCT GCTGGACATA 
ACGCCCAATG CAGTGGATCG TCTCAACTAC GCCCAGTTCT ATCCGGTGGT GATCTTCCTG 
AAGACAGACA GCAAGCACGT GATCAAGCAG CTGCGCCACG GACTACCCAA GGCGGCCCAC 
AAGAGCTCCA AGAAGCTGCT GGAGCAGACC CAGAAACTGG AGCGCGTCTG GTCCCACATC 
TTTAGCACCC AGATCGCGTT GAGCGACGAG GAGTCCTGGT ACCGAAAGTT GCGCGACTCG 
ATCGATCTGC AGCAGAGCGG CGCCGTGTGG ATGTCCGAGT CCAAGCCAGT AGAATCCCTC 
TCCGACGATT TCCTATTCCC CATGACCACA TCCCGACTCT CGTATGCATC AAGTCCCGAA 
TCCGATTTGG AACTGAGTCC CGGCCCATCC GCATCATTGT CGCTTGGCAA TTTGCCGCAG 
TTGGTTAAAG CGAGCTCAGA TCCATCGATT GCCACTAATC AGGATAACTT GGATAGGGAT 
AGAGACATAA TTGGTGAAGG ACTACCACCT CCATATACGG TACCCTACGA CCATGGTGTC 
CCCGCGAATC CCAATCGCCG CCAGACCATG GACTCCAGCA AGTACAGCAT CTACGGCACC 
AACGTGCCGC CGCAGCCGCA GCAGCCCGGC GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA 
CAGTCTCTGT ACGGGATTAA TGCACCCGAT CTGCCGCCCC GCATCGATCG TCAGTCCAAG 
CCGGGTGATA TGCCACTGAA CACCTCGGGC TCGTCGTCGC GAAATGGCAC CCTGGGTCGC 
AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT GTGGTGCAGG ACGATGTCCA GGCGGAATAC 
ATAACCCGAA ATGCTCTGGT CGGCTCCGGG GCGGCTGAAA CCCTGGATCG CCAGCAGCAG 
CAGCAAACCC ACGCGTCCTT GGAGCGGCAG GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC 
GGAGGAGGCG CCGGAGGAGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC 
AACAACACAC AAATGGCCAT GCAAAACCTG GGCCGACTGG GTCCCAATGC ACCCGACGAT 
CTCAAATCAG TGCCAAATGC AAGTGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCTCGAC 
TACGGACGCA CTCCGCACGA TCACCGCAGC TTCGGAGGAG CCAACGATCT GAACCGACAG 
AGCAGTCCGG GCAGACCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT TGATCCACGC 
AATGGCACTC CACAGCGTCC TTCGAATCTG GGCCTAGAAA GCAGTCCACG CAAGCCTTTG 
GTGGAGACGA AGACGGATTA CGGCAAATAC AGTCGAAACA ACTCCGTATC GCAAGCGGAT 
TACACCAAGC TACCGAAGAC GGCGCCACAT GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC 
GGCCAAAGCC AGATGAATGG CAGTGGAACG CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG 
CCGGTGCCGC CACCCAAACC CAAGAACTAT AGGCCACCGG TGCAAAGTGG TGGCAGCAGT 
GGCAGCGGTG GCACTACTCC GTGGGAAAAT GGGGACTCTG GCTCGCCACG CTCTCCAAAT 
GGCTTCTATT ATCCACCAAC GCCTTCGCAT CATCACTACG GCCAGCAGGC GACTCCCGGT 
TCACCCAGCA ACGGCCACAT GCAGCCACCT CCGCCGCAGC AGCAGCAGCA GCAGCCCACC 
TACGGCGGAA GTAATGGCAA CTACGGACAG GCGCCACCAC CACAACCCTA TCCGCAGGCC 
AATGGCTACA ACGGGAATGG CCATCACTAC AACGGAGGCA GCGGCACGGG ACCCTACATT 
GCACCACATC GCGGCATGCC GCCGCCAATA GGAAACCTGC CGCCCCACAC GCCCGAACGT 
CATGCCCTGG ACTTGGCCGG AAGTCGGGAG CAGCGTGGCT CGGCCTTCGA GCTGTATCGT 
AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC CATCACAACA TGAGCGAAAT GGAGCCGTAC 
GACGAGCGAT ACGATGATTA CTATAACATG CCACCCCCTG CCGCCCATCC ATCCCAAGGG 
CACCATATGC AGCGCTCCCG ATCAGCTCCT CGTTATCCCC AGGAGCGACC TCCTCACGCA 
CAAGATCCGA ATTACTACGG CCACTATGGA ACTTCTAGGG GACATAGTCA GCCGCGCCAG 
CAGTATCCTC AGCATCACCA GCAACAGTAT TACGATGAGC ATGGCATGGA GATGGGTCCA 
CCGCCACTGC CCCCACATAA GAAGAAGAAA TCGGTGCTGA AGAGCCCACT GGTGGCCCTC 
AAGAATGCGC TGCTTAAGAG CACGCGGCCC CTGAGGAGAA TGAACAGCAT GGTGGAGCCG 
GAGAGGAAAC CGAAGGGACT CCGACGCCAG CAATCGATGC TGGAGAGGGG TGTGCAAAGA 
CCCTACTATC CGGATGAGTA TCCAACCTAT CCAGCTGGCT TTGAGGAGCG TGCCCATGGC 
GGACAAGGCA TGATGCAGCC TCATCCGCAA GATGCCTACT ATCAAAGGGG TGGTCACTAC 
ACGTCGCAGC AGCAGGAGAT GATGAACAGC ACCTACCAGA ATCTGGAGGG TGAGGATATC 
TATGGGAACA TCGGAAACAC AGTTCCTAGA ATGCCGCATC ACCAGGACAA TGGTTACGGA 
TATGACCAAT ACGATCTCTA TGCCAATCGA GCGTGCATCG ATCTCGAAAG AAGGCAAGCA 
GAGGCAGCTG CAGCTTCCGG TGGCAGAAAT GGGAGGAGAA TTGTGAGGCG GCACAGCACC 
ACCACTGCGG ATCGAGGAGG AAATCCCGGA CCGCCCAGGA GGCCTATGAT CAGTCCTGGT 
TATGAGCAGG ATCCGCAAGA GATCTACCAG ACCCGCAACG GAGCCTACAT GCTGCCCGAT 
CAGCGAAGGG CGCCCAGTTC GGAGGTGATG ACCCGCAGAA GATTCTATCC GGGGGCTGCC 
AATGAGCAAA CTGAGGAGGA GCCCCTCTAC CAATCCCGTC GCGAAATGCA GCGTGAGATG 
CAGAGGAATC ATCTGTACCA ATCGAAACGG GAAATGCAGG AGAGGATTAG CCAGGGCAAG 
AGGGATATGG AGCGAGAGTT TAGCCCCCAG GGCAGCAGCA GCCAGTCGGA GACCTCCAAT 
CCGGAGGTAA TATACCAGAG TCGCCGGGAG GCCAAGGAAA GTGCTCTGAA AACGAGGGCA 
CAACTGAGGG ATCAGATCTA TCAGACTAGA CGGGAAGCCC TGGACAGCAT GGCGGAACCG 
ATCTATGTGT CCAAAAGAGA TATGGGCAGA CCGGCTCCTA TCTATGAGAC TCGGGAAGAG 
AGTATCCTCC AGTCCCGAGA AAATGAAACC GACGAAAAGA AGGAAAAGGA GTGCAAGACC 
GAGCAGATTC AGGTGGAGAT TAATGCTCAA CCGGAGGAAC AGGTGGAAGA TTCGACCTTA 
AGTCGATCCG ACTTGCAGAA ATCTTCGGAC ACGGTGATAG AAAATCCAGC GAGAGCTCCT 
CTTGTGCAGG ATGAGGTAGA TGACGACGAA GAAGAGCAGG CTCAGGCTCG GGATGATCCC 
GCTGAAAGCT CTGCGGATCA GACAGAGCTG CCAACGGCCA TTGAAAACAA TCTACACAAC 
GAGGGAATGG TGGAGCAAAC ACAGAATGAG TCGGATGACG TATTCGAAGC AGCCGATAAG 
GTTTCTCCTC TAGCCGCTCC TACAGGTACT GTTCAACCGC CGACCCCGCG ATCCAGTAGA 
GCTCCCTTCC ACATTTCGAA TATCCTAAAG CGAACAGCAC CTCCTCCGAG TTCCCCAATG 
GGCGACAGCT GCACCTCCAT CGAAACCCAG TACACATCCC AGGCCAGCCT GCCAGTGGGC 
CCACCAAATG CCACCAGTAC GCCGTTCAGT AGCAGCATGT CATTGCCCAT CGCCGGACCC 
GTAAACAATC AACCGCCGGC CAGCGGACCC TTTCCCACTC TGCCCCGCGA ACCCAGCACC 
TCGCGCGGAT TCTTTGACTC CAATGGTGGC ACTCTGGCAG ATAAGCTGTG GCACGTCTCA 
CTGCAAATCC CGCCGGGAGC CATTCCAGCC GGAGTACGGC AAGAGATCTA CTTCACCGTG 
AGCGATCCGC GAATGGGACA AGCCGTCGGC GGTCCTCCGC TGGACATGGA AAATGGTGAG 
ACAATGCTGT CACCGCTGGT GATGTGCGGG CCGCAGGGCC TTGAGTTCCT GGTACCGGTT 
ACACTCAACA TACCGCACTG CGCCGGACGA ACTGCATCAC TGGGACTGGC CCTCAAGGCG 
ACCGACAGCG AGAAGAACCT GCACACCGAG TGGGACAACA TCGACCTGCC CAGCAATGCC 
GCCGCCCACA CGGTCTCCGT CAAGGTGGAT CACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010028.1
CDS	231..7409
Translation	MKPSSTRRRSFKTKPPKSNVYNPLVRYRPTAYVRNGIIGGGEGPGSSGVYQINDRVYTTSKMFLRDGYMYPRQPGQTMDPRFPLELHEFPQQFGATGLQLVSAPKVHIAGEYLANEQQQQQQQQQHQQVQEQQQLGRRKAAAAAEINLANQVYWLGKQKTRSRSRSNSVGNRSITSCMGGGGGGGDKERERVGDRDRPYIRNVDDLLQALGKRDGDNTSNLLETASTHSSSQRRRFKKGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_015154542.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant D (Dere\GG17413), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154542.1

ORF Insert Sequence:

ATGAAGCCCA GCAGCACGCG ACGTCGCAGT TTCAAGACGA AACCCCCCAA ATCGAATGTT 
TATAACCCGC TGGTTCGCTA TCGCCCTACG GCCTATGTCC GGAACGGAAT TATTGGCGGC 
GGCGAGGGTC CCGGCTCGAG TGGTGTGTAC CAAATCAACG ATAGGGTGTA TACCACCAGC 
AAGATGTTTC TGCGCGACGG CTATATGTAC CCGCGTCAAC CGGGTCAAAC CATGGATCCC 
CGTTTCCCCC TCGAGTTGCA CGAGTTCCCT CAGCAATTCG GCGCCACTGG CCTGCAGTTG 
GTTAGTGCAC CCAAGGTTCA TATAGCCGGC GAATATCTGG CCAATGAACA GCAGCAGCAA 
CAACAGCAGC AGCAGCATCA ACAGGTGCAG GAGCAACAAC AATTGGGCAG AAGAAAGGCT 
GCAGCAGCCG CGGAAATAAA TTTGGCAAAT CAGGTTTACT GGCTGGGCAA GCAAAAGACC 
AGATCCCGAT CCCGGAGCAA TAGTGTGGGC AATCGGAGCA TAACCAGTTG CATGGGCGGC 
GGTGGAGGGG GTGGCGATAA GGAAAGGGAA CGCGTCGGTG ATCGGGATCG TCCCTATATA 
CGGAACGTTG ACGATCTTCT CCAGGCACTG GGCAAAAGGG ATGGGGATAA CACCAGCAAC 
CTGCTCGAAA CTGCTTCGAC GCACTCTTCA TCGCAGCGAA GGCGCTTCAA GAAGGGTGAT 
CGAACCACTT GGGAGTACCA CACGGTGGCA GTGACTCGGG TGCCGGGCTA TGGATTCGGC 
ATCGCCGTTT CCGGTGGACG TGATAATCCG CACTTCGCCA ACGGCGATCC CTCGATAGCA 
GTTAGCGATG TGCTGAAAGG TGGCCCCGCC GAGGATCGAT TGCAAGTCAA CGATCGGATA 
ATCTCGGTGA ACGGCGTCTC CCTGGAGAAC GTGGAATATG CCACCGCGGT GCAGGTGTTG 
CGCGATAGTG GCAACACGGT CCAATTGGTG GTCAAGCGTC GCGTGCCCCT CAATCCCATC 
AATAATGGAG GTGCAGTGCA GCATCAGCAC TCGCACAGTC TCAGCTCGGT TGGTCTATTG 
GCCAATGGAA GTGGAGGAGT TGCACCAACG CCCTTGACGA GCCTGAGTCA ACCCAACTCC 
CTGAACTCTT CGCTGGTGCA GAATGCCAGC AGTGGTCAGC CCATCAAGGT GACCCTCACG 
AAAGGCGGCA AGAAAGATGA CTATGGCGTG GTCCTTGGCT GCCGGCTGTT TGTTAAGGAG 
ATCTCCTCCA AAGCTCGCGA GCAACTTAAT GCCAATGGCT ACAGTTTGCA AGAGGGTGAC 
ATTATCACCC GCATCCACAA CACCAATTGC GGGGATACGA TGAGTCTCAA GGAGGCGAAA 
AAGATCATCG ATGGTTGCAA GGAGCGCTTG AACCTAGTGG TTCTACGGGA CATCACCAAC 
CAAGCAGCTG TTAGCCAACT GAATCTGAAC AATTCAGCGA GCCACCAGGC TTCGGCAAAT 
ATATACGGCA CTCATCAGCC TCAGGTATCC GGATGTAGCA GCAGTAACAA CAATCTTGAA 
GATCCATATC TGCCGGGCGG AGCCAGTTAC TCCTCGCAGA ATCTGTACGT GCAACCACCC 
ACTCGCACCT CCAATGGTCC CAATCTCAAT GGCAATGGGC TGAACGACGA AAAGAGTAAT 
CTCACACCGC GAGGTCGCTC CCGGGGGCCC ATTATGGATG GGGTATCTCT GCAGCAGCTG 
GACAGGCCCG TAACACCGAC GCGTGGCAGG AGTGCGGCCA TCGACGAGCC GCCACGTCCA 
CCGCCGCCAC GAGGATCGAG CGGGGGAGCG GCCCAGGAGG ATTTCTACAG CTCTCGCAGG 
CAATTGTACG AGGAGCGCCA GAGTGCCGAG CCGAGATTCA TTTCCTTCCA GAAGGAGGGA 
AGCGTGGGCA TCCGCCTAAC CGGCGGCAAT GAAGCCGGTA TTTTTGTGAC TGCCGTACAA 
CCGGGATCTC CAGCCTCGCT GCAGGGTCTA ATGCCAGGCG ATAAGATCCT GAAGGTCAAC 
GACATGGACA TGAACGGAGT GACGCGCGAG GAGGCTGTTC TCTTTCTGTT GAGCCTGCAG 
GATCGCATCG ATCTGATTGT GCAGTACTGC AAGGAGGAGT ACGACGAGGT GGTGACCAAC 
CAACGTGGCG ACTCCTTCCA CATCAAGACA CATTTCCACT GCGATAATCC CTCCAAAGGC 
GAGATGGCCT TCAAGGCGGG CGATGTGTTC CGGGTGATCG ATACCCTGCA CAATGGTGTA 
GTCGGTTCCT GGCAGGTGCT GAAGATCGGA CGTGGTCACC AGGAGATGCA GCGCGGCGTT 
ATACCGAACA AGTCGCGGGC CGAAGAACTG GCCACTGCCC AGTTCAATGC CACCAAGAAG 
GAGATGAACG CCAACGAGTC GAGGGGCAAC TTCTTCCGGC GACGACGCTC GACGCACCGA 
CGCTCGAAGA GTCTGTCCCG CGAGAACTGG GACGACGTGG TCTTCTCCGA CAGCATTTCC 
AAATTCCCCG CCTACGAGCG AGTCGTTCTC CGCCATCCTG GCTTTGTTCG ACCCGTAGTG 
CTCTTCGGAC CCGTATCCGA TCTGGCCCGC GAGCGCTTGG CCAAGGATTT CCCCGACAAG 
TTTTCGACTC CACTGCAGGA TGATGACAAG TCAGCTGCAA CCAGTGGAAA GTGCCGCATC 
GTAAGGCTCT CGAACATACG CGATGTGATG GATCGTGGCA AGCACGCCCT GCTGGACATA 
ACGCCCAATG CAGTGGATCG TCTCAACTAC GCCCAGTTCT ATCCGGTGGT GATCTTCCTG 
AAGACAGACA GCAAGCACGT GATCAAGCAG CTGCGCCACG GACTACCCAA GGCGGCCCAC 
AAGAGCTCCA AGAAGCTGCT GGAGCAGACC CAGAAACTGG AGCGCGTCTG GTCCCACATC 
TTTAGCACCC AGATCGCGTT GAGCGACGAG GAGTCCTGGT ACCGAAAGTT GCGCGACTCG 
ATCGATCTGC AGCAGAGCGG CGCCGTGTGG ATGTCCGAGT CCAAGCCAGT AGAATCCCTC 
TCCGACGATT TCCTATTCCC CATGACCACA TCCCGACTCT CGTATGCATC AAGTCCCGAA 
TCCGATTTGG AACTGAGTCC CGGCCCATCC GCATCATTGT CGCTTGGCAA TTTGCCGCAG 
TTGGTTAAAG CGAGCTCAGA TCCATCGATT GCCACTAATC AGGATAACTT GGATAGGGAT 
AGAGACATAA TTGGTGAAGG ACTACCACCT CCATATACGG TACCCTACGA CCATGGTGTC 
CCCGCGAATC CCAATCGCCG CCAGACCATG GACTCCAGCA AGTACAGCAT CTACGGCACC 
AACGTGCCGC CGCAGCCGCA GCAGCCCGGC GCAGGAGGAG ATCCGGCTGC AGTGAGGCCA 
CAGTCTCTGT ACGGGATTAA TGCACCCGAT CTGCCGCCCC GCATCGATCG TCAGTCCAAG 
CCGGGTGATA TGCCACTGAA CACCTCGGGC TCGTCGTCGC GAAATGGCAC CCTGGGTCGC 
AGTGCCCAGG AGCGTCTGTT TGGCAAGGCT GTGGTGCAGG ACGATGTCCA GGCGGAATAC 
ATAACCCGAA ATGCTCTGGT CGGCTCCGGG GCGGCTGAAA CCCTGGATCG CCAGCAGCAG 
CAGCAAACCC ACGCGTCCTT GGAGCGGCAG GCGCGTTTGA ATGCGCAGCT GAAGGCCAAC 
GGAGGAGGCG CCGGAGGAGG TGCATCCACC TACGACAGTG TGTCCAGCTA CGACTCGTAC 
AACAACACAC AAATGGCCAT GCAAAACCTG GGCCGACTGG GTCCCAATGC ACCCGACGAT 
CTCAAATCAG TGCCAAATGC AAGTGGTCGC CCCTTGCCTC CGACGGGTCA GTCGCTCGAC 
TACGGACGCA CTCCGCACGA TCACCGCAGC TTCGGAGGAG CCAACGATCT GAACCGACAG 
AGCAGTCCGG GCAGACCGCA CTACCATGAC ATGAATGCGT CCCGTAATAT TGATCCACGC 
AATGGCACTC CACAGCGTCC TTCGAATCTG GGCCTAGAAA GCAGTCCACG CAAGCCTTTG 
GTGGAGACGA AGACGGATTA CGGCAAATAC AGTCGAAACA ACTCCGTATC GCAAGCGGAT 
TACACCAAGC TACCGAAGAC GGCGCCACAT GGAGTGGTAC CGCCGCCGAA TGTGAGCAAC 
GGCCAAAGCC AGATGAATGG CAGTGGAACG CCCAGCAGCA ATGGCAGTGG ACCCTTCAAG 
CCGGTGCCGC CACCCAAACC CAAGAACTAT AGGCCACCGG TGCAAAGTGG TGGCAGCAGT 
GGCAGCGGTG GCACTACTCC GTGGGAAAAT GGGGACTCTG GCTCGCCACG CTCTCCAAAT 
GGCTTCTATT ATCCACCAAC GCCTTCGCAT CATCACTACG GCCAGCAGGC GACTCCCGGT 
TCACCCAGCA ACGGCCACAT GCAGCCACCT CCGCCGCAGC AGCAGCAGCA GCAGCCCACC 
TACGGCGGAA GTAATGGCAA CTACGGACAG GCGCCACCAC CACAACCCTA TCCGCAGGCC 
AATGGCTACA ACGGGAATGG CCATCACTAC AACGGAGGCA GCGGCACGGG ACCCTACATT 
GCACCACATC GCGGCATGCC GCCGCCAATA GGAAACCTGC CGCCCCACAC GCCCGAACGT 
CATGCCCTGG ACTTGGCCGG AAGTCGGGAG CAGCGTGGCT CGGCCTTCGA GCTGTATCGT 
AAGCCGCAAA TCGGAGCAAC CGCTGGGCAC CATCACAACA TGAGCGAAAT GGAGCCGTAC 
GACGAGCGAT ACGATGATTA CTATAACATG CCACCCCCTG CCGCCCATCC ATCCCAAGGG 
CACCATATGC AGCGCTCCCG ATCAGCTCCT CGTTATCCCC AGGAGCGACC TCCTCACGCA 
CAAGATCCGA ATTACTACGG CCACTATGGA ACTTCTAGGG GACATAGTCA GCCGCGCCAG 
CAGTATCCTC AGCATCACCA GCAACAGTAT TACGATGAGC ATGGCATGGA GATGGGTCCA 
CCGCCACTGC CCCCACATAA GAAGAAGAAA TCGGTGCTGA AGAGCCCACT GGTGGCCCTC 
AAGAATGCGC TGCTTAAGAG CACGCGGCCC CTGAGGAGAA TGAACAGCAT GGTGGAGCCG 
GAGAGGAAAC CGAAGGGACT CCGACGCCAG CAATCGATGC TGGAGAGGGG TGTGCAAAGA 
CCCTACTATC CGGATGAGTA TCCAACCTAT CCAGCTGGCT TTGAGGAGCG TGCCCATGGC 
GGACAAGGCA TGATGCAGCC TCATCCGCAA GATGCCTACT ATCAAAGGGG TGGTCACTAC 
ACGTCGCAGC AGCAGGAGAT GATGAACAGC ACCTACCAGA ATCTGGAGGG TGAGGATATC 
TATGGGAACA TCGGAAACAC AGTTCCTAGA ATGCCGCATC ACCAGGACAA TGGTTACGGA 
TATGACCAAT ACGATCTCTA TGCCAATCGA GCGTGCATCG ATCTCGAAAG AAGGCAAGCA 
GAGGCAGCTG CAGCTTCCGG TGGCAGAAAT GGGAGGAGAA TTGTGAGGCG GCACAGCACC 
ACCACTGCGG ATCGAGGAGG AAATCCCGGA CCGCCCAGGA GGCCTATGAT CAGTCCTGGT 
TATGAGCAGG ATCCGCAAGA GATCTACCAG ACCCGCAACG GAGCCTACAT GCTGCCCGAT 
CAGCGAAGGG CGCCCAGTTC GGAGGTGATG ACCCGCAGAA GATTCTATCC GGGGGCTGCC 
AATGAGCAAA CTGAGGAGGA GCCCCTCTAC CAATCCCGTC GCGAAATGCA GCGTGAGATG 
CAGAGGAATC ATCTGTACCA ATCGAAACGG GAAATGCAGG AGAGGATTAG CCAGGGCAAG 
AGGGATATGG AGCGAGAGTT TAGCCCCCAG GGCAGCAGCA GCCAGTCGGA GACCTCCAAT 
CCGGAGGTAA TATACCAGAG TCGCCGGGAG GCCAAGGAAA GTGCTCTGAA AACGAGGGCA 
CAACTGAGGG ATCAGATCTA TCAGACTAGA CGGGAAGCCC TGGACAGCAT GGCGGAACCG 
ATCTATGTGT CCAAAAGAGA TATGGGCAGA CCGGCTCCTA TCTATGAGAC TCGGGAAGAG 
AGTATCCTCC AGTCCCGAGA AAATGAAACC GACGAAAAGA AGGAAAAGGA GTGCAAGACC 
GAGCAGATTC AGGTGGAGAT TAATGCTCAA CCGGAGGAAC AGGTGGAAGA TTCGACCTTA 
AGTCGATCCG ACTTGCAGAA ATCTTCGGAC ACGGTGATAG AAAATCCAGC GAGAGCTCCT 
CTTGTGCAGG ATGAGGTAGA TGACGACGAA GAAGAGCAGG CTCAGGCTCG GGATGATCCC 
GCTGAAAGCT CTGCGGATCA GACAGAGCTG CCAACGGCCA TTGAAAACAA TCTACACAAC 
GAGGGAATGG TGGAGCAAAC ACAGAATGAG TCGGATGACG TATTCGAAGC AGCCGATAAG 
GTTTCTCCTC TAGCCGCTCC TACAGGTACT GTTCAACCGC CGACCCCGCG ATCCAGTAGA 
GCTCCCTTCC ACATTTCGAA TATCCTAAAG CGAACAGCAC CTCCTCCGAG TTCCCCAATG 
GGCGACAGCT GCACCTCCAT CGAAACCCAG TACACATCCC AGGCCAGCCT GCCAGTGGGC 
CCACCAAATG CCACCAGTAC GCCGTTCAGT AGCAGCATGT CATTGCCCAT CGCCGGACCC 
GTAAACAATC AACCGCCGGC CAGCGGACCC TTTCCCACTC TGCCCCGCGA ACCCAGCACC 
TCGCGCGGAT TCTTTGACTC CAATGGTGGC ACTCTGGCAG ATAAGCTGTG GCACGTCTCA 
CTGCAAATCC CGCCGGGAGC CATTCCAGCC GGAGTACGGC AAGAGATCTA CTTCACCGTG 
AGCGATCCGC GAATGGGACA AGCCGTCGGC GGTCCTCCGC TGGACATGGA AAATGGTGAG 
ACAATGCTGT CACCGCTGGT GATGTGCGGG CCGCAGGGCC TTGAGTTCCT GGTACCGGTT 
ACACTCAACA TACCGCACTG CGCCGGACGA ACTGCATCAC TGGGACTGGC CCTCAAGGCG 
ACCGACAGCG AGAAGAACCT GCACACCGAG TGGGACAACA TCGACCTGCC CAGCAATGCC 
GCCGCCCACA CGGTCTCCGT CAAGGTGGAT CACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154543.1 ) cDNA ORF clone, Drosophila erecta -> XP_015010029.1 Drosophila erecta uncharacterized protein, transcript variant E (Dere\GG17413), mRNA.

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CloneID	ODi33930
Clone ID Related Accession (Same CDS sequence)	XM_015154543.1
Accession Version	XM_015154543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956552). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCAGT TCGACTATAC CTGCTATACA GTGCCCGAGG GTTCCACGGA TGACGACTGC 
GATGAGCTGG GCAGCAATGC CACCCTGGAG AACAAGGATC ACTCGGATTG CACCCTGGAA 
TCGGATGCTA CGACCACCAC ATCTTCACTG GAGCGTCATA GATATCGGAA TCGGTATCGC 
TCCACCAGGA GTTTCGGTCC CCAGCGGGTT TCCATGCCCG TTCTCGGATC CAGCCGGTCC 
AGGGAGATGA CGCCCCAGTT GGGAGTGTAC CATGAGGGAC CGGATCGAGA TCCCCAATGG 
GGTTACTTTG TACCCCTGTC GCCCAACTGC TTCTTTAGTC CACCGATGCA GAGGAGGCGT 
TATAGGGAAA AGGAAAACGA TCGCAATCGT CGGGCTGTTA GTTATGGCGG TGACGTCACC 
TCCTCCCCCC ACTCGGCGAC CATACCCCGC GCCGCCCCCC GTCGTGTGGC CTTGATGAAG 
GATCCACCGC CACCGCCGCC ACCCAAGCCG CAATTGGGCT CCAAAGCGGA TTCCGTAGCG 
CATTTGGCCA GCAAATATCC CCAATCCGAG TACAATCTCA TACAGAAAAT CGACTCGAAC 
AGCACACTCA CTGCTCCGGC GCAATATCAA CCGCAGAATT TCTACGCGAA TACCGGAACC 
ATATCCTCCA CGAATGGCTA TGGATCGCTG TTGTGTCACG CCAGTTCGAC GACCAGTCCG 
CTGGCGGTTC GGAAGCGGGA CAAGCTGCTC CATCGGTTCA GCGATGCCGC CACCTTGGGC 
CGGAAGCTCA AGAAGAAGAA GAACACGAAT CGCACCTGCA GATCCATGAC GGAGGCTATT 
GAGATGCTGG CCGATCCCGT CATCGAGGAT GAATTCTTTG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCTCGACGC ACCGACGCTC GAAGAGTCTG 
TCCCGCGAGA ACTGGGACGA CGTGGTCTTC TCCGACAGCA TTTCCAAATT CCCCGCCTAC 
GAGCGAGTCG TTCTCCGCCA TCCTGGCTTT GTTCGACCCG TAGTGCTCTT CGGACCCGTA 
TCCGATCTGG CCCGCGAGCG CTTGGCCAAG GATTTCCCCG ACAAGTTTTC GACTCCACTG 
CAGGATGATG ACAAGTCAGC TGCAACCAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAC 
ATACGCGATG TGATGGATCG TGGCAAGCAC GCCCTGCTGG ACATAACGCC CAATGCAGTG 
GATCGTCTCA ACTACGCCCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG 
CACGTGATCA AGCAGCTGCG CCACGGACTA CCCAAGGCGG CCCACAAGAG CTCCAAGAAG 
CTGCTGGAGC AGACCCAGAA ACTGGAGCGC GTCTGGTCCC ACATCTTTAG CACCCAGATC 
GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ACTCGATCGA TCTGCAGCAG 
AGCGGCGCCG TGTGGATGTC CGAGTCCAAG CCAGTAGAAT CCCTCTCCGA CGATTTCCTA 
TTCCCCATGA CCACATCCCG ACTCTCGTAT GCATCAAGTC CCGAATCCGA TTTGGAACTG 
AGTCCCGGCC CATCCGCATC ATTGTCGCTT GGCAATTTGC CGCAGTTGGT TAAAGCGAGC 
TCAGATCCAT CGATTGCCAC TAATCAGGAT AACTTGGATA GGGATAGAGA CATAATTGGT 
GAAGGACTAC CACCTCCATA TACGGTACCC TACGACCATG GTGTCCCCGC GAATCCCAAT 
CGCCGCCAGA CCATGGACTC CAGCAAGTAC AGCATCTACG GCACCAACGT GCCGCCGCAG 
CCGCAGCAGC CCGGCGCAGG AGGAGATCCG GCTGCAGTGA GGCCACAGTC TCTGTACGGG 
ATTAATGCAC CCGATCTGCC GCCCCGCATC GATCGTCAGT CCAAGCCGGG TGATATGCCA 
CTGAACACCT CGGGCTCGTC GTCGCGAAAT GGCACCCTGG GTCGCAGTGC CCAGGAGCGT 
CTGTTTGGCA AGGCTGTGGT GCAGGACGAT GTCCAGGCGG AATACATAAC CCGAAATGCT 
CTGGTCGGCT CCGGGGCGGC TGAAACCCTG GATCGCCAGC AGCAGCAGCA AACCCACGCG 
TCCTTGGAGC GGCAGGCGCG TTTGAATGCG CAGCTGAAGG CCAACGGAGG AGGCGCCGGA 
GGAGGTGCAT CCACCTACGA CAGTGTGTCC AGCTACGACT CGTACAACAA CACACAAATG 
GCCATGCAAA ACCTGGGCCG ACTGGGTCCC AATGCACCCG ACGATCTCAA ATCAGTGCCA 
AATGCAAGTG GTCGCCCCTT GCCTCCGACG GGTCAGTCGC TCGACTACGG ACGCACTCCG 
CACGATCACC GCAGCTTCGG AGGAGCCAAC GATCTGAACC GACAGAGCAG TCCGGGCAGA 
CCGCACTACC ATGACATGAA TGCGTCCCGT AATATTGATC CACGCAATGG CACTCCACAG 
CGTCCTTCGA ATCTGGGCCT AGAAAGCAGT CCACGCAAGC CTTTGGTGGA GACGAAGACG 
GATTACGGCA AATACAGTCG AAACAACTCC GTATCGCAAG CGGATTACAC CAAGCTACCG 
AAGACGGCGC CACATGGAGT GGTACCGCCG CCGAATGTGA GCAACGGCCA AAGCCAGATG 
AATGGCAGTG GAACGCCCAG CAGCAATGGC AGTGGACCCT TCAAGCCGGT GCCGCCACCC 
AAACCCAAGA ACTATAGGCC ACCGGTGCAA AGTGGTGGCA GCAGTGGCAG CGGTGGCACT 
ACTCCGTGGG AAAATGGGGA CTCTGGCTCG CCACGCTCTC CAAATGGCTT CTATTATCCA 
CCAACGCCTT CGCATCATCA CTACGGCCAG CAGGCGACTC CCGGTTCACC CAGCAACGGC 
CACATGCAGC CACCTCCGCC GCAGCAGCAG CAGCAGCAGC CCACCTACGG CGGAAGTAAT 
GGCAACTACG GACAGGCGCC ACCACCACAA CCCTATCCGC AGGCCAATGG CTACAACGGG 
AATGGCCATC ACTACAACGG AGGCAGCGGC ACGGGACCCT ACATTGCACC ACATCGCGGC 
ATGCCGCCGC CAATAGGAAA CCTGCCGCCC CACACGCCCG AACGTCATGC CCTGGACTTG 
GCCGGAAGTC GGGAGCAGCG TGGCTCGGCC TTCGAGCTGT ATCGTAAGCC GCAAATCGGA 
GCAACCGCTG GGCACCATCA CAACATGAGC GAAATGGAGC CGTACGACGA GCGATACGAT 
GATTACTATA ACATGCCACC CCCTGCCGCC CATCCATCCC AAGGGCACCA TATGCAGCGC 
TCCCGATCAG CTCCTCGTTA TCCCCAGGAG CGACCTCCTC ACGCACAAGA TCCGAATTAC 
TACGGCCACT ATGGAACTTC TAGGGGACAT AGTCAGCCGC GCCAGCAGTA TCCTCAGCAT 
CACCAGCAAC AGTATTACGA TGAGCATGGC ATGGAGATGG GTCCACCGCC ACTGCCCCCA 
CATAAGAAGA AGAAATCGGT GCTGAAGAGC CCACTGGTGG CCCTCAAGAA TGCGCTGCTT 
AAGAGCACGC GGCCCCTGAG GAGAATGAAC AGCATGGTGG AGCCGGAGAG GAAACCGAAG 
GGACTCCGAC GCCAGCAATC GATGCTGGAG AGGGGTGTGC AAAGACCCTA CTATCCGGAT 
GAGTATCCAA CCTATCCAGC TGGCTTTGAG GAGCGTGCCC ATGGCGGACA AGGCATGATG 
CAGCCTCATC CGCAAGATGC CTACTATCAA AGGGGTGGTC ACTACACGTC GCAGCAGCAG 
GAGATGATGA ACAGCACCTA CCAGAATCTG GAGGGTGAGG ATATCTATGG GAACATCGGA 
AACACAGTTC CTAGAATGCC GCATCACCAG GACAATGGTT ACGGATATGA CCAATACGAT 
CTCTATGCCA ATCGAGCGTG CATCGATCTC GAAAGAAGGC AAGCAGAGGC AGCTGCAGCT 
TCCGGTGGCA GAAATGGGAG GAGAATTGTG AGGCGGCACA GCACCACCAC TGCGGATCGA 
GGAGGAAATC CCGGACCGCC CAGGAGGCCT ATGATCAGTC CTGGTTATGA GCAGGATCCG 
CAAGAGATCT ACCAGACCCG CAACGGAGCC TACATGCTGC CCGATCAGCG AAGGGCGCCC 
AGTTCGGAGG TGATGACCCG CAGAAGATTC TATCCGGGGG CTGCCAATGA GCAAACTGAG 
GAGGAGCCCC TCTACCAATC CCGTCGCGAA ATGCAGCGTG AGATGCAGAG GAATCATCTG 
TACCAATCGA AACGGGAAAT GCAGGAGAGG ATTAGCCAGG GCAAGAGGGA TATGGAGCGA 
GAGTTTAGCC CCCAGGGCAG CAGCAGCCAG TCGGAGACCT CCAATCCGGA GGTAATATAC 
CAGAGTCGCC GGGAGGCCAA GGAAAGTGCT CTGAAAACGA GGGCACAACT GAGGGATCAG 
ATCTATCAGA CTAGACGGGA AGCCCTGGAC AGCATGGCGG AACCGATCTA TGTGTCCAAA 
AGAGATATGG GCAGACCGGC TCCTATCTAT GAGACTCGGG AAGAGAGTAT CCTCCAGTCC 
CGAGAAAATG AAACCGACGA AAAGAAGGAA AAGGAGTGCA AGACCGAGCA GATTCAGGTG 
GAGATTAATG CTCAACCGGA GGAACAGGTG GAAGATTCGA CCTTAAGTCG ATCCGACTTG 
CAGAAATCTT CGGACACGGT GATAGAAAAT CCAGCGAGAG CTCCTCTTGT GCAGGATGAG 
GTAGATGACG ACGAAGAAGA GCAGGCTCAG GCTCGGGATG ATCCCGCTGA AAGCTCTGCG 
GATCAGACAG AGCTGCCAAC GGCCATTGAA AACAATCTAC ACAACGAGGG AATGGTGGAG 
CAAACACAGA ATGAGTCGGA TGACGTATTC GAAGCAGCCG ATAAGGTTTC TCCTCTAGCC 
GCTCCTACAG GTACTGTTCA ACCGCCGACC CCGCGATCCA GTAGAGCTCC CTTCCACATT 
TCGAATATCC TAAAGCGAAC AGCACCTCCT CCGAGTTCCC CAATGGGCGA CAGCTGCACC 
TCCATCGAAA CCCAGTACAC ATCCCAGGCC AGCCTGCCAG TGGGCCCACC AAATGCCACC 
AGTACGCCGT TCAGTAGCAG CATGTCATTG CCCATCGCCG GACCCGTAAA CAATCAACCG 
CCGGCCAGCG GACCCTTTCC CACTCTGCCC CGCGAACCCA GCACCTCGCG CGGATTCTTT 
GACTCCAATG GTGGCACTCT GGCAGATAAG CTGTGGCACG TCTCACTGCA AATCCCGCCG 
GGAGCCATTC CAGCCGGAGT ACGGCAAGAG ATCTACTTCA CCGTGAGCGA TCCGCGAATG 
GGACAAGCCG TCGGCGGTCC TCCGCTGGAC ATGGAAAATG GTGAGACAAT GCTGTCACCG 
CTGGTGATGT GCGGGCCGCA GGGCCTTGAG TTCCTGGTAC CGGTTACACT CAACATACCG 
CACTGCGCCG GACGAACTGC ATCACTGGGA CTGGCCCTCA AGGCGACCGA CAGCGAGAAG 
AACCTGCACA CCGAGTGGGA CAACATCGAC CTGCCCAGCA ATGCCGCCGC CCACACGGTC 
TCCGTCAAGG TGGATCACTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010029.1
CDS	119..7462
Translation	MSQFDYTCYTVPEGSTDDDCDELGSNATLENKDHSDCTLESDATTTTSSLERHRYRNRYRSTRSFGPQRVSMPVLGSSRSREMTPQLGVYHEGPDRDPQWGYFVPLSPNCFFSPPMQRRRYREKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_015154543.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant E (Dere\GG17413), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154543.1

ORF Insert Sequence:

ATGTCGCAGT TCGACTATAC CTGCTATACA GTGCCCGAGG GTTCCACGGA TGACGACTGC 
GATGAGCTGG GCAGCAATGC CACCCTGGAG AACAAGGATC ACTCGGATTG CACCCTGGAA 
TCGGATGCTA CGACCACCAC ATCTTCACTG GAGCGTCATA GATATCGGAA TCGGTATCGC 
TCCACCAGGA GTTTCGGTCC CCAGCGGGTT TCCATGCCCG TTCTCGGATC CAGCCGGTCC 
AGGGAGATGA CGCCCCAGTT GGGAGTGTAC CATGAGGGAC CGGATCGAGA TCCCCAATGG 
GGTTACTTTG TACCCCTGTC GCCCAACTGC TTCTTTAGTC CACCGATGCA GAGGAGGCGT 
TATAGGGAAA AGGAAAACGA TCGCAATCGT CGGGCTGTTA GTTATGGCGG TGACGTCACC 
TCCTCCCCCC ACTCGGCGAC CATACCCCGC GCCGCCCCCC GTCGTGTGGC CTTGATGAAG 
GATCCACCGC CACCGCCGCC ACCCAAGCCG CAATTGGGCT CCAAAGCGGA TTCCGTAGCG 
CATTTGGCCA GCAAATATCC CCAATCCGAG TACAATCTCA TACAGAAAAT CGACTCGAAC 
AGCACACTCA CTGCTCCGGC GCAATATCAA CCGCAGAATT TCTACGCGAA TACCGGAACC 
ATATCCTCCA CGAATGGCTA TGGATCGCTG TTGTGTCACG CCAGTTCGAC GACCAGTCCG 
CTGGCGGTTC GGAAGCGGGA CAAGCTGCTC CATCGGTTCA GCGATGCCGC CACCTTGGGC 
CGGAAGCTCA AGAAGAAGAA GAACACGAAT CGCACCTGCA GATCCATGAC GGAGGCTATT 
GAGATGCTGG CCGATCCCGT CATCGAGGAT GAATTCTTTG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCTCGACGC ACCGACGCTC GAAGAGTCTG 
TCCCGCGAGA ACTGGGACGA CGTGGTCTTC TCCGACAGCA TTTCCAAATT CCCCGCCTAC 
GAGCGAGTCG TTCTCCGCCA TCCTGGCTTT GTTCGACCCG TAGTGCTCTT CGGACCCGTA 
TCCGATCTGG CCCGCGAGCG CTTGGCCAAG GATTTCCCCG ACAAGTTTTC GACTCCACTG 
CAGGATGATG ACAAGTCAGC TGCAACCAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAC 
ATACGCGATG TGATGGATCG TGGCAAGCAC GCCCTGCTGG ACATAACGCC CAATGCAGTG 
GATCGTCTCA ACTACGCCCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG 
CACGTGATCA AGCAGCTGCG CCACGGACTA CCCAAGGCGG CCCACAAGAG CTCCAAGAAG 
CTGCTGGAGC AGACCCAGAA ACTGGAGCGC GTCTGGTCCC ACATCTTTAG CACCCAGATC 
GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ACTCGATCGA TCTGCAGCAG 
AGCGGCGCCG TGTGGATGTC CGAGTCCAAG CCAGTAGAAT CCCTCTCCGA CGATTTCCTA 
TTCCCCATGA CCACATCCCG ACTCTCGTAT GCATCAAGTC CCGAATCCGA TTTGGAACTG 
AGTCCCGGCC CATCCGCATC ATTGTCGCTT GGCAATTTGC CGCAGTTGGT TAAAGCGAGC 
TCAGATCCAT CGATTGCCAC TAATCAGGAT AACTTGGATA GGGATAGAGA CATAATTGGT 
GAAGGACTAC CACCTCCATA TACGGTACCC TACGACCATG GTGTCCCCGC GAATCCCAAT 
CGCCGCCAGA CCATGGACTC CAGCAAGTAC AGCATCTACG GCACCAACGT GCCGCCGCAG 
CCGCAGCAGC CCGGCGCAGG AGGAGATCCG GCTGCAGTGA GGCCACAGTC TCTGTACGGG 
ATTAATGCAC CCGATCTGCC GCCCCGCATC GATCGTCAGT CCAAGCCGGG TGATATGCCA 
CTGAACACCT CGGGCTCGTC GTCGCGAAAT GGCACCCTGG GTCGCAGTGC CCAGGAGCGT 
CTGTTTGGCA AGGCTGTGGT GCAGGACGAT GTCCAGGCGG AATACATAAC CCGAAATGCT 
CTGGTCGGCT CCGGGGCGGC TGAAACCCTG GATCGCCAGC AGCAGCAGCA AACCCACGCG 
TCCTTGGAGC GGCAGGCGCG TTTGAATGCG CAGCTGAAGG CCAACGGAGG AGGCGCCGGA 
GGAGGTGCAT CCACCTACGA CAGTGTGTCC AGCTACGACT CGTACAACAA CACACAAATG 
GCCATGCAAA ACCTGGGCCG ACTGGGTCCC AATGCACCCG ACGATCTCAA ATCAGTGCCA 
AATGCAAGTG GTCGCCCCTT GCCTCCGACG GGTCAGTCGC TCGACTACGG ACGCACTCCG 
CACGATCACC GCAGCTTCGG AGGAGCCAAC GATCTGAACC GACAGAGCAG TCCGGGCAGA 
CCGCACTACC ATGACATGAA TGCGTCCCGT AATATTGATC CACGCAATGG CACTCCACAG 
CGTCCTTCGA ATCTGGGCCT AGAAAGCAGT CCACGCAAGC CTTTGGTGGA GACGAAGACG 
GATTACGGCA AATACAGTCG AAACAACTCC GTATCGCAAG CGGATTACAC CAAGCTACCG 
AAGACGGCGC CACATGGAGT GGTACCGCCG CCGAATGTGA GCAACGGCCA AAGCCAGATG 
AATGGCAGTG GAACGCCCAG CAGCAATGGC AGTGGACCCT TCAAGCCGGT GCCGCCACCC 
AAACCCAAGA ACTATAGGCC ACCGGTGCAA AGTGGTGGCA GCAGTGGCAG CGGTGGCACT 
ACTCCGTGGG AAAATGGGGA CTCTGGCTCG CCACGCTCTC CAAATGGCTT CTATTATCCA 
CCAACGCCTT CGCATCATCA CTACGGCCAG CAGGCGACTC CCGGTTCACC CAGCAACGGC 
CACATGCAGC CACCTCCGCC GCAGCAGCAG CAGCAGCAGC CCACCTACGG CGGAAGTAAT 
GGCAACTACG GACAGGCGCC ACCACCACAA CCCTATCCGC AGGCCAATGG CTACAACGGG 
AATGGCCATC ACTACAACGG AGGCAGCGGC ACGGGACCCT ACATTGCACC ACATCGCGGC 
ATGCCGCCGC CAATAGGAAA CCTGCCGCCC CACACGCCCG AACGTCATGC CCTGGACTTG 
GCCGGAAGTC GGGAGCAGCG TGGCTCGGCC TTCGAGCTGT ATCGTAAGCC GCAAATCGGA 
GCAACCGCTG GGCACCATCA CAACATGAGC GAAATGGAGC CGTACGACGA GCGATACGAT 
GATTACTATA ACATGCCACC CCCTGCCGCC CATCCATCCC AAGGGCACCA TATGCAGCGC 
TCCCGATCAG CTCCTCGTTA TCCCCAGGAG CGACCTCCTC ACGCACAAGA TCCGAATTAC 
TACGGCCACT ATGGAACTTC TAGGGGACAT AGTCAGCCGC GCCAGCAGTA TCCTCAGCAT 
CACCAGCAAC AGTATTACGA TGAGCATGGC ATGGAGATGG GTCCACCGCC ACTGCCCCCA 
CATAAGAAGA AGAAATCGGT GCTGAAGAGC CCACTGGTGG CCCTCAAGAA TGCGCTGCTT 
AAGAGCACGC GGCCCCTGAG GAGAATGAAC AGCATGGTGG AGCCGGAGAG GAAACCGAAG 
GGACTCCGAC GCCAGCAATC GATGCTGGAG AGGGGTGTGC AAAGACCCTA CTATCCGGAT 
GAGTATCCAA CCTATCCAGC TGGCTTTGAG GAGCGTGCCC ATGGCGGACA AGGCATGATG 
CAGCCTCATC CGCAAGATGC CTACTATCAA AGGGGTGGTC ACTACACGTC GCAGCAGCAG 
GAGATGATGA ACAGCACCTA CCAGAATCTG GAGGGTGAGG ATATCTATGG GAACATCGGA 
AACACAGTTC CTAGAATGCC GCATCACCAG GACAATGGTT ACGGATATGA CCAATACGAT 
CTCTATGCCA ATCGAGCGTG CATCGATCTC GAAAGAAGGC AAGCAGAGGC AGCTGCAGCT 
TCCGGTGGCA GAAATGGGAG GAGAATTGTG AGGCGGCACA GCACCACCAC TGCGGATCGA 
GGAGGAAATC CCGGACCGCC CAGGAGGCCT ATGATCAGTC CTGGTTATGA GCAGGATCCG 
CAAGAGATCT ACCAGACCCG CAACGGAGCC TACATGCTGC CCGATCAGCG AAGGGCGCCC 
AGTTCGGAGG TGATGACCCG CAGAAGATTC TATCCGGGGG CTGCCAATGA GCAAACTGAG 
GAGGAGCCCC TCTACCAATC CCGTCGCGAA ATGCAGCGTG AGATGCAGAG GAATCATCTG 
TACCAATCGA AACGGGAAAT GCAGGAGAGG ATTAGCCAGG GCAAGAGGGA TATGGAGCGA 
GAGTTTAGCC CCCAGGGCAG CAGCAGCCAG TCGGAGACCT CCAATCCGGA GGTAATATAC 
CAGAGTCGCC GGGAGGCCAA GGAAAGTGCT CTGAAAACGA GGGCACAACT GAGGGATCAG 
ATCTATCAGA CTAGACGGGA AGCCCTGGAC AGCATGGCGG AACCGATCTA TGTGTCCAAA 
AGAGATATGG GCAGACCGGC TCCTATCTAT GAGACTCGGG AAGAGAGTAT CCTCCAGTCC 
CGAGAAAATG AAACCGACGA AAAGAAGGAA AAGGAGTGCA AGACCGAGCA GATTCAGGTG 
GAGATTAATG CTCAACCGGA GGAACAGGTG GAAGATTCGA CCTTAAGTCG ATCCGACTTG 
CAGAAATCTT CGGACACGGT GATAGAAAAT CCAGCGAGAG CTCCTCTTGT GCAGGATGAG 
GTAGATGACG ACGAAGAAGA GCAGGCTCAG GCTCGGGATG ATCCCGCTGA AAGCTCTGCG 
GATCAGACAG AGCTGCCAAC GGCCATTGAA AACAATCTAC ACAACGAGGG AATGGTGGAG 
CAAACACAGA ATGAGTCGGA TGACGTATTC GAAGCAGCCG ATAAGGTTTC TCCTCTAGCC 
GCTCCTACAG GTACTGTTCA ACCGCCGACC CCGCGATCCA GTAGAGCTCC CTTCCACATT 
TCGAATATCC TAAAGCGAAC AGCACCTCCT CCGAGTTCCC CAATGGGCGA CAGCTGCACC 
TCCATCGAAA CCCAGTACAC ATCCCAGGCC AGCCTGCCAG TGGGCCCACC AAATGCCACC 
AGTACGCCGT TCAGTAGCAG CATGTCATTG CCCATCGCCG GACCCGTAAA CAATCAACCG 
CCGGCCAGCG GACCCTTTCC CACTCTGCCC CGCGAACCCA GCACCTCGCG CGGATTCTTT 
GACTCCAATG GTGGCACTCT GGCAGATAAG CTGTGGCACG TCTCACTGCA AATCCCGCCG 
GGAGCCATTC CAGCCGGAGT ACGGCAAGAG ATCTACTTCA CCGTGAGCGA TCCGCGAATG 
GGACAAGCCG TCGGCGGTCC TCCGCTGGAC ATGGAAAATG GTGAGACAAT GCTGTCACCG 
CTGGTGATGT GCGGGCCGCA GGGCCTTGAG TTCCTGGTAC CGGTTACACT CAACATACCG 
CACTGCGCCG GACGAACTGC ATCACTGGGA CTGGCCCTCA AGGCGACCGA CAGCGAGAAG 
AACCTGCACA CCGAGTGGGA CAACATCGAC CTGCCCAGCA ATGCCGCCGC CCACACGGTC 
TCCGTCAAGG TGGATCACTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154544.1 ) cDNA ORF clone, Drosophila erecta -> XP_015010030.1 Drosophila erecta uncharacterized protein, transcript variant G (Dere\GG17413), mRNA.

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CloneID	ODi33931
Clone ID Related Accession (Same CDS sequence)	XM_015154544.1
Accession Version	XM_015154544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956552). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCTCGACGC ACCGACGCTC GAAGAGTCTG 
TCCCGCGAGA ACTGGGACGA CGTGGTCTTC TCCGACAGCA TTTCCAAATT CCCCGCCTAC 
GAGCGAGTCG TTCTCCGCCA TCCTGGCTTT GTTCGACCCG TAGTGCTCTT CGGACCCGTA 
TCCGATCTGG CCCGCGAGCG CTTGGCCAAG GATTTCCCCG ACAAGTTTTC GACTCCACTG 
CAGGATGATG ACAAGTCAGC TGCAACCAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAC 
ATACGCGATG TGATGGATCG TGGCAAGCAC GCCCTGCTGG ACATAACGCC CAATGCAGTG 
GATCGTCTCA ACTACGCCCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG 
CACGTGATCA AGCAGCTGCG CCACGGACTA CCCAAGGCGG CCCACAAGAG CTCCAAGAAG 
CTGCTGGAGC AGACCCAGAA ACTGGAGCGC GTCTGGTCCC ACATCTTTAG CACCCAGATC 
GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ACTCGATCGA TCTGCAGCAG 
AGCGGCGCCG TGTGGATGTC CGAGTCCAAG GTACCCTACG ACCATGGTGT CCCCGCGAAT 
CCCAATCGCC GCCAGACCAT GGACTCCAGC AAGTACAGCA TCTACGGCAC CAACGTGCCG 
CCGCAGCCGC AGCAGCCCGG CGCAGGAGGA GATCCGGCTG CAGTGAGGCC ACAGTCTCTG 
TACGGGATTA ATGCACCCGA TCTGCCGCCC CGCATCGATC GTCAGTCCAA GCCGGGTGAT 
ATGCCACTGA ACACCTCGGG CTCGTCGTCG CGAAATGGCA CCCTGGGTCG CAGTGCCCAG 
GAGCGTCTGT TTGGCAAGGC TGTGGTGCAG GACGATGTCC AGGCGGAATA CATAACCCGA 
AATGCTCTGG TCGGCTCCGG GGCGGCTGAA ACCCTGGATC GCCAGCAGCA GCAGCAAACC 
CACGCGTCCT TGGAGCGGCA GGCGCGTTTG AATGCGCAGC TGAAGGCCAA CGGAGGAGGC 
GCCGGAGGAG GTGCATCCAC CTACGACAGT GTGTCCAGCT ACGACTCGTA CAACAACACA 
CAAATGGCCA TGCAAAACCT GGGCCGACTG GGTCCCAATG CACCCGACGA TCTCAAATCA 
GTGCCAAATG CAAGTGGTCG CCCCTTGCCT CCGACGGGTC AGTCGCTCGA CTACGGACGC 
ACTCCGCACG ATCACCGCAG CTTCGGAGGA GCCAACGATC TGAACCGACA GAGCAGTCCG 
GGCAGACCGC ACTACCATGA CATGAATGCG TCCCGTAATA TTGATCCACG CAATGGCACT 
CCACAGCGTC CTTCGAATCT GGGCCTAGAA AGCAGTCCAC GCAAGCCTTT GGTGGAGACG 
AAGACGGATT ACGGCAAATA CAGTCGAAAC AACTCCGTAT CGCAAGCGGA TTACACCAAG 
CTACCGAAGA CGGCGCCACA TGGAGTGGTA CCGCCGCCGA ATGTGAGCAA CGGCCAAAGC 
CAGATGAATG GCAGTGGAAC GCCCAGCAGC AATGGCAGTG GACCCTTCAA GCCGGTGCCG 
CCACCCAAAC CCAAGAACTA TAGGCCACCG GTGCAAAGTG GTGGCAGCAG TGGCAGCGGT 
GGCACTACTC CGTGGGAAAA TGGGGACTCT GGCTCGCCAC GCTCTCCAAA TGGCTTCTAT 
TATCCACCAA CGCCTTCGCA TCATCACTAC GGCCAGCAGG CGACTCCCGG TTCACCCAGC 
AACGGCCACA TGCAGCCACC TCCGCCGCAG CAGCAGCAGC AGCAGCCCAC CTACGGCGGA 
AGTAATGGCA ACTACGGACA GGCGCCACCA CCACAACCCT ATCCGCAGGC CAATGGCTAC 
AACGGGAATG GCCATCACTA CAACGGAGGC AGCGGCACGG GACCCTACAT TGCACCACAT 
CGCGGCATGC CGCCGCCAAT AGGAAACCTG CCGCCCCACA CGCCCGAACG TCATGCCCTG 
GACTTGGCCG GAAGTCGGGA GCAGCGTGGC TCGGCCTTCG AGCTGTATCG TAAGCCGCAA 
ATCGGAGCAA CCGCTGGGCA CCATCACAAC ATGAGCGAAA TGGAGCCGTA CGACGAGCGA 
TACGATGATT ACTATAACAT GCCACCCCCT GCCGCCCATC CATCCCAAGG GCACCATATG 
CAGCGCTCCC GATCAGCTCC TCGTTATCCC CAGGAGCGAC CTCCTCACGC ACAAGATCCG 
AATTACTACG GCCACTATGG AACTTCTAGG GGACATAGTC AGCCGCGCCA GCAGTATCCT 
CAGCATCACC AGCAACAGTA TTACGATGAG CATGGCATGG AGATGGGTCC ACCGCCACTG 
CCCCCACATA AGAAGAAGAA ATCGGTGCTG AAGAGCCCAC TGGTGGCCCT CAAGAATGCG 
CTGCTTAAGA GCACGCGGCC CCTGAGGAGA ATGAACAGCA TGGTGGAGCC GGAGAGGAAA 
CCGAAGGGAC TCCGACGCCA GCAATCGATG CTGGAGAGGG GTGTGCAAAG ACCCTACTAT 
CCGGATGAGT ATCCAACCTA TCCAGCTGGC TTTGAGGAGC GTGCCCATGG CGGACAAGGC 
ATGATGCAGC CTCATCCGCA AGATGCCTAC TATCAAAGGG GTGGTCACTA CACGTCGCAG 
CAGCAGGAGA TGATGAACAG CACCTACCAG AATCTGGAGG GTGAGGATAT CTATGGGAAC 
ATCGGAAACA CAGTTCCTAG AATGCCGCAT CACCAGGACA ATGGTTACGG ATATGACCAA 
TACGATCTCT ATGCCAATCG AGCGTGCATC GATCTCGAAA GAAGGCAAGC AGAGGCAGCT 
GCAGCTTCCG GTGGCAGAAA TGGGAGGAGA ATTGTGAGGC GGCACAGCAC CACCACTGCG 
GATCGAGGAG GAAATCCCGG ACCGCCCAGG AGGCCTATGA TCAGTCCTGG TTATGAGCAG 
GATCCGCAAG AGATCTACCA GACCCGCAAC GGAGCCTACA TGCTGCCCGA TCAGCGAAGG 
GCGCCCAGTT CGGAGGTGAT GACCCGCAGA AGATTCTATC CGGGGGCTGC CAATGAGCAA 
ACTGAGGAGG AGCCCCTCTA CCAATCCCGT CGCGAAATGC AGCGTGAGAT GCAGAGGAAT 
CATCTGTACC AATCGAAACG GGAAATGCAG GAGAGGATTA GCCAGGGCAA GAGGGATATG 
GAGCGAGAGT TTAGCCCCCA GGGCAGCAGC AGCCAGTCGG AGACCTCCAA TCCGGAGGTA 
ATATACCAGA GTCGCCGGGA GGCCAAGGAA AGTGCTCTGA AAACGAGGGC ACAACTGAGG 
GATCAGATCT ATCAGACTAG ACGGGAAGCC CTGGACAGCA TGGCGGAACC GATCTATGTG 
TCCAAAAGAG ATATGGGCAG ACCGGCTCCT ATCTATGAGA CTCGGGAAGA GAGTATCCTC 
CAGTCCCGAG AAAATGAAAC CGACGAAAAG AAGGAAAAGG AGTGCAAGAC CGAGCAGATT 
CAGGTGGAGA TTAATGCTCA ACCGGAGGAA CAGGTGGAAG ATTCGACCTT AAGTCGATCC 
GACTTGCAGA AATCTTCGGA CACGGTGATA GAAAATCCAG CGAGAGCTCC TCTTGTGCAG 
GATGAGGTAG ATGACGACGA AGAAGAGCAG GCTCAGGCTC GGGATGATCC CGCTGAAAGC 
TCTGCGGATC AGACAGAGCT GCCAACGGCC ATTGAAAACA ATCTACACAA CGAGGGAATG 
GTGGAGCAAA CACAGAATGA GTCGGATGAC GTATTCGAAG CAGCCGATAA GGTTTCTCCT 
CTAGCCGCTC CTACAGGTAC TGTTCAACCG CCGACCCCGC GATCCAGTAG AGCTCCCTTC 
CACATTTCGA ATATCCTAAA GCGAACAGCA CCTCCTCCGA GTTCCCCAAT GGGCGACAGC 
TGCACCTCCA TCGAAACCCA GTACACATCC CAGGCCAGCC TGCCAGTGGG CCCACCAAAT 
GCCACCAGTA CGCCGTTCAG TAGCAGCATG TCATTGCCCA TCGCCGGACC CGTAAACAAT 
CAACCGCCGG CCAGCGGACC CTTTCCCACT CTGCCCCGCG AACCCAGCAC CTCGCGCGGA 
TTCTTTGACT CCAATGGTGG CACTCTGGCA GATAAGCTGT GGCACGTCTC ACTGCAAATC 
CCGCCGGGAG CCATTCCAGC CGGAGTACGG CAAGAGATCT ACTTCACCGT GAGCGATCCG 
CGAATGGGAC AAGCCGTCGG CGGTCCTCCG CTGGACATGG AAAATGGTGA GACAATGCTG 
TCACCGCTGG TGATGTGCGG GCCGCAGGGC CTTGAGTTCC TGGTACCGGT TACACTCAAC 
ATACCGCACT GCGCCGGACG AACTGCATCA CTGGGACTGG CCCTCAAGGC GACCGACAGC 
GAGAAGAACC TGCACACCGA GTGGGACAAC ATCGACCTGC CCAGCAATGC CGCCGCCCAC 
ACGGTCTCCG TCAAGGTGGA TCACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010030.1
CDS	298..6567
Translation	MKFPDYVPNNMLVGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_015154544.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant G (Dere\GG17413), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154544.1

ORF Insert Sequence:

ATGAAGTTTC CGGATTATGT TCCAAACAAC ATGTTGGTGG GTGATCGAAC CACTTGGGAG 
TACCACACGG TGGCAGTGAC TCGGGTGCCG GGCTATGGAT TCGGCATCGC CGTTTCCGGT 
GGACGTGATA ATCCGCACTT CGCCAACGGC GATCCCTCGA TAGCAGTTAG CGATGTGCTG 
AAAGGTGGCC CCGCCGAGGA TCGATTGCAA GTCAACGATC GGATAATCTC GGTGAACGGC 
GTCTCCCTGG AGAACGTGGA ATATGCCACC GCGGTGCAGG TGTTGCGCGA TAGTGGCAAC 
ACGGTCCAAT TGGTGGTCAA GCGTCGCGTG CCCCTCAATC CCATCAATAA TGGAGGTGCA 
GTGCAGCATC AGCACTCGCA CAGTCTCAGC TCGGTTGGTC TATTGGCCAA TGGAAGTGGA 
GGAGTTGCAC CAACGCCCTT GACGAGCCTG AGTCAACCCA ACTCCCTGAA CTCTTCGCTG 
GTGCAGAATG CCAGCAGTGG TCAGCCCATC AAGGTGACCC TCACGAAAGG CGGCAAGAAA 
GATGACTATG GCGTGGTCCT TGGCTGCCGG CTGTTTGTTA AGGAGATCTC CTCCAAAGCT 
CGCGAGCAAC TTAATGCCAA TGGCTACAGT TTGCAAGAGG GTGACATTAT CACCCGCATC 
CACAACACCA ATTGCGGGGA TACGATGAGT CTCAAGGAGG CGAAAAAGAT CATCGATGGT 
TGCAAGGAGC GCTTGAACCT AGTGGTTCTA CGGGACATCA CCAACCAAGC AGCTGTTAGC 
CAACTGAATC TGAACAATTC AGCGAGCCAC CAGGCTTCGG CAAATATATA CGGCACTCAT 
CAGCCTCAGG TATCCGGATG TAGCAGCAGT AACAACAATC TTGAAGATCC ATATCTGCCG 
GGCGGAGCCA GTTACTCCTC GCAGAATCTG TACGTGCAAC CACCCACTCG CACCTCCAAT 
GGTCCCAATC TCAATGGCAA TGGGCTGAAC GACGAAAAGA GTAATCTCAC ACCGCGAGGT 
CGCTCCCGGG GGCCCATTAT GGATGGGGTA TCTCTGCAGC AGCTGGACAG GCCCGTAACA 
CCGACGCGTG GCAGGAGTGC GGCCATCGAC GAGCCGCCAC GTCCACCGCC GCCACGAGGA 
TCGAGCGGGG GAGCGGCCCA GGAGGATTTC TACAGCTCTC GCAGGCAATT GTACGAGGAG 
CGCCAGAGTG CCGAGCCGAG ATTCATTTCC TTCCAGAAGG AGGGAAGCGT GGGCATCCGC 
CTAACCGGCG GCAATGAAGC CGGTATTTTT GTGACTGCCG TACAACCGGG ATCTCCAGCC 
TCGCTGCAGG GTCTAATGCC AGGCGATAAG ATCCTGAAGG TCAACGACAT GGACATGAAC 
GGAGTGACGC GCGAGGAGGC TGTTCTCTTT CTGTTGAGCC TGCAGGATCG CATCGATCTG 
ATTGTGCAGT ACTGCAAGGA GGAGTACGAC GAGGTGGTGA CCAACCAACG TGGCGACTCC 
TTCCACATCA AGACACATTT CCACTGCGAT AATCCCTCCA AAGGCGAGAT GGCCTTCAAG 
GCGGGCGATG TGTTCCGGGT GATCGATACC CTGCACAATG GTGTAGTCGG TTCCTGGCAG 
GTGCTGAAGA TCGGACGTGG TCACCAGGAG ATGCAGCGCG GCGTTATACC GAACAAGTCG 
CGGGCCGAAG AACTGGCCAC TGCCCAGTTC AATGCCACCA AGAAGGAGAT GAACGCCAAC 
GAGTCGAGGG GCAACTTCTT CCGGCGACGA CGCTCGACGC ACCGACGCTC GAAGAGTCTG 
TCCCGCGAGA ACTGGGACGA CGTGGTCTTC TCCGACAGCA TTTCCAAATT CCCCGCCTAC 
GAGCGAGTCG TTCTCCGCCA TCCTGGCTTT GTTCGACCCG TAGTGCTCTT CGGACCCGTA 
TCCGATCTGG CCCGCGAGCG CTTGGCCAAG GATTTCCCCG ACAAGTTTTC GACTCCACTG 
CAGGATGATG ACAAGTCAGC TGCAACCAGT GGAAAGTGCC GCATCGTAAG GCTCTCGAAC 
ATACGCGATG TGATGGATCG TGGCAAGCAC GCCCTGCTGG ACATAACGCC CAATGCAGTG 
GATCGTCTCA ACTACGCCCA GTTCTATCCG GTGGTGATCT TCCTGAAGAC AGACAGCAAG 
CACGTGATCA AGCAGCTGCG CCACGGACTA CCCAAGGCGG CCCACAAGAG CTCCAAGAAG 
CTGCTGGAGC AGACCCAGAA ACTGGAGCGC GTCTGGTCCC ACATCTTTAG CACCCAGATC 
GCGTTGAGCG ACGAGGAGTC CTGGTACCGA AAGTTGCGCG ACTCGATCGA TCTGCAGCAG 
AGCGGCGCCG TGTGGATGTC CGAGTCCAAG GTACCCTACG ACCATGGTGT CCCCGCGAAT 
CCCAATCGCC GCCAGACCAT GGACTCCAGC AAGTACAGCA TCTACGGCAC CAACGTGCCG 
CCGCAGCCGC AGCAGCCCGG CGCAGGAGGA GATCCGGCTG CAGTGAGGCC ACAGTCTCTG 
TACGGGATTA ATGCACCCGA TCTGCCGCCC CGCATCGATC GTCAGTCCAA GCCGGGTGAT 
ATGCCACTGA ACACCTCGGG CTCGTCGTCG CGAAATGGCA CCCTGGGTCG CAGTGCCCAG 
GAGCGTCTGT TTGGCAAGGC TGTGGTGCAG GACGATGTCC AGGCGGAATA CATAACCCGA 
AATGCTCTGG TCGGCTCCGG GGCGGCTGAA ACCCTGGATC GCCAGCAGCA GCAGCAAACC 
CACGCGTCCT TGGAGCGGCA GGCGCGTTTG AATGCGCAGC TGAAGGCCAA CGGAGGAGGC 
GCCGGAGGAG GTGCATCCAC CTACGACAGT GTGTCCAGCT ACGACTCGTA CAACAACACA 
CAAATGGCCA TGCAAAACCT GGGCCGACTG GGTCCCAATG CACCCGACGA TCTCAAATCA 
GTGCCAAATG CAAGTGGTCG CCCCTTGCCT CCGACGGGTC AGTCGCTCGA CTACGGACGC 
ACTCCGCACG ATCACCGCAG CTTCGGAGGA GCCAACGATC TGAACCGACA GAGCAGTCCG 
GGCAGACCGC ACTACCATGA CATGAATGCG TCCCGTAATA TTGATCCACG CAATGGCACT 
CCACAGCGTC CTTCGAATCT GGGCCTAGAA AGCAGTCCAC GCAAGCCTTT GGTGGAGACG 
AAGACGGATT ACGGCAAATA CAGTCGAAAC AACTCCGTAT CGCAAGCGGA TTACACCAAG 
CTACCGAAGA CGGCGCCACA TGGAGTGGTA CCGCCGCCGA ATGTGAGCAA CGGCCAAAGC 
CAGATGAATG GCAGTGGAAC GCCCAGCAGC AATGGCAGTG GACCCTTCAA GCCGGTGCCG 
CCACCCAAAC CCAAGAACTA TAGGCCACCG GTGCAAAGTG GTGGCAGCAG TGGCAGCGGT 
GGCACTACTC CGTGGGAAAA TGGGGACTCT GGCTCGCCAC GCTCTCCAAA TGGCTTCTAT 
TATCCACCAA CGCCTTCGCA TCATCACTAC GGCCAGCAGG CGACTCCCGG TTCACCCAGC 
AACGGCCACA TGCAGCCACC TCCGCCGCAG CAGCAGCAGC AGCAGCCCAC CTACGGCGGA 
AGTAATGGCA ACTACGGACA GGCGCCACCA CCACAACCCT ATCCGCAGGC CAATGGCTAC 
AACGGGAATG GCCATCACTA CAACGGAGGC AGCGGCACGG GACCCTACAT TGCACCACAT 
CGCGGCATGC CGCCGCCAAT AGGAAACCTG CCGCCCCACA CGCCCGAACG TCATGCCCTG 
GACTTGGCCG GAAGTCGGGA GCAGCGTGGC TCGGCCTTCG AGCTGTATCG TAAGCCGCAA 
ATCGGAGCAA CCGCTGGGCA CCATCACAAC ATGAGCGAAA TGGAGCCGTA CGACGAGCGA 
TACGATGATT ACTATAACAT GCCACCCCCT GCCGCCCATC CATCCCAAGG GCACCATATG 
CAGCGCTCCC GATCAGCTCC TCGTTATCCC CAGGAGCGAC CTCCTCACGC ACAAGATCCG 
AATTACTACG GCCACTATGG AACTTCTAGG GGACATAGTC AGCCGCGCCA GCAGTATCCT 
CAGCATCACC AGCAACAGTA TTACGATGAG CATGGCATGG AGATGGGTCC ACCGCCACTG 
CCCCCACATA AGAAGAAGAA ATCGGTGCTG AAGAGCCCAC TGGTGGCCCT CAAGAATGCG 
CTGCTTAAGA GCACGCGGCC CCTGAGGAGA ATGAACAGCA TGGTGGAGCC GGAGAGGAAA 
CCGAAGGGAC TCCGACGCCA GCAATCGATG CTGGAGAGGG GTGTGCAAAG ACCCTACTAT 
CCGGATGAGT ATCCAACCTA TCCAGCTGGC TTTGAGGAGC GTGCCCATGG CGGACAAGGC 
ATGATGCAGC CTCATCCGCA AGATGCCTAC TATCAAAGGG GTGGTCACTA CACGTCGCAG 
CAGCAGGAGA TGATGAACAG CACCTACCAG AATCTGGAGG GTGAGGATAT CTATGGGAAC 
ATCGGAAACA CAGTTCCTAG AATGCCGCAT CACCAGGACA ATGGTTACGG ATATGACCAA 
TACGATCTCT ATGCCAATCG AGCGTGCATC GATCTCGAAA GAAGGCAAGC AGAGGCAGCT 
GCAGCTTCCG GTGGCAGAAA TGGGAGGAGA ATTGTGAGGC GGCACAGCAC CACCACTGCG 
GATCGAGGAG GAAATCCCGG ACCGCCCAGG AGGCCTATGA TCAGTCCTGG TTATGAGCAG 
GATCCGCAAG AGATCTACCA GACCCGCAAC GGAGCCTACA TGCTGCCCGA TCAGCGAAGG 
GCGCCCAGTT CGGAGGTGAT GACCCGCAGA AGATTCTATC CGGGGGCTGC CAATGAGCAA 
ACTGAGGAGG AGCCCCTCTA CCAATCCCGT CGCGAAATGC AGCGTGAGAT GCAGAGGAAT 
CATCTGTACC AATCGAAACG GGAAATGCAG GAGAGGATTA GCCAGGGCAA GAGGGATATG 
GAGCGAGAGT TTAGCCCCCA GGGCAGCAGC AGCCAGTCGG AGACCTCCAA TCCGGAGGTA 
ATATACCAGA GTCGCCGGGA GGCCAAGGAA AGTGCTCTGA AAACGAGGGC ACAACTGAGG 
GATCAGATCT ATCAGACTAG ACGGGAAGCC CTGGACAGCA TGGCGGAACC GATCTATGTG 
TCCAAAAGAG ATATGGGCAG ACCGGCTCCT ATCTATGAGA CTCGGGAAGA GAGTATCCTC 
CAGTCCCGAG AAAATGAAAC CGACGAAAAG AAGGAAAAGG AGTGCAAGAC CGAGCAGATT 
CAGGTGGAGA TTAATGCTCA ACCGGAGGAA CAGGTGGAAG ATTCGACCTT AAGTCGATCC 
GACTTGCAGA AATCTTCGGA CACGGTGATA GAAAATCCAG CGAGAGCTCC TCTTGTGCAG 
GATGAGGTAG ATGACGACGA AGAAGAGCAG GCTCAGGCTC GGGATGATCC CGCTGAAAGC 
TCTGCGGATC AGACAGAGCT GCCAACGGCC ATTGAAAACA ATCTACACAA CGAGGGAATG 
GTGGAGCAAA CACAGAATGA GTCGGATGAC GTATTCGAAG CAGCCGATAA GGTTTCTCCT 
CTAGCCGCTC CTACAGGTAC TGTTCAACCG CCGACCCCGC GATCCAGTAG AGCTCCCTTC 
CACATTTCGA ATATCCTAAA GCGAACAGCA CCTCCTCCGA GTTCCCCAAT GGGCGACAGC 
TGCACCTCCA TCGAAACCCA GTACACATCC CAGGCCAGCC TGCCAGTGGG CCCACCAAAT 
GCCACCAGTA CGCCGTTCAG TAGCAGCATG TCATTGCCCA TCGCCGGACC CGTAAACAAT 
CAACCGCCGG CCAGCGGACC CTTTCCCACT CTGCCCCGCG AACCCAGCAC CTCGCGCGGA 
TTCTTTGACT CCAATGGTGG CACTCTGGCA GATAAGCTGT GGCACGTCTC ACTGCAAATC 
CCGCCGGGAG CCATTCCAGC CGGAGTACGG CAAGAGATCT ACTTCACCGT GAGCGATCCG 
CGAATGGGAC AAGCCGTCGG CGGTCCTCCG CTGGACATGG AAAATGGTGA GACAATGCTG 
TCACCGCTGG TGATGTGCGG GCCGCAGGGC CTTGAGTTCC TGGTACCGGT TACACTCAAC 
ATACCGCACT GCGCCGGACG AACTGCATCA CTGGGACTGG CCCTCAAGGC GACCGACAGC 
GAGAAGAACC TGCACACCGA GTGGGACAAC ATCGACCTGC CCAGCAATGC CGCCGCCCAC 
ACGGTCTCCG TCAAGGTGGA TCACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6552588 ( XM_015154545.1 ) cDNA ORF clone, Drosophila erecta -> XP_015010031.1 Drosophila erecta uncharacterized protein, transcript variant H (Dere\GG17413), mRNA.

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CloneID	ODi33932
Clone ID Related Accession (Same CDS sequence)	XM_015154545.1
Accession Version	XM_015154545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform H
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956552). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGGTACCCTA CGACCATGGT 
GTCCCCGCGA ATCCCAATCG CCGCCAGACC ATGGACTCCA GCAAGTACAG CATCTACGGC 
ACCAACGTGC CGCCGCAGCC GCAGCAGCCC GGCGCAGGAG GAGATCCGGC TGCAGTGAGG 
CCACAGTCTC TGTACGGGAT TAATGCACCC GATCTGCCGC CCCGCATCGA TCGTCAGTCC 
AAGCCGGGTG ATATGCCACT GAACACCTCG GGCTCGTCGT CGCGAAATGG CACCCTGGGT 
CGCAGTGCCC AGGAGCGTCT GTTTGGCAAG GCTGTGGTGC AGGACGATGT CCAGGCGGAA 
TACATAACCC GAAATGCTCT GGTCGGCTCC GGGGCGGCTG AAACCCTGGA TCGCCAGCAG 
CAGCAGCAAA CCCACGCGTC CTTGGAGCGG CAGGCGCGTT TGAATGCGCA GCTGAAGGCC 
AACGGAGGAG GCGCCGGAGG AGGTGCATCC ACCTACGACA GTGTGTCCAG CTACGACTCG 
TACAACAACA CACAAATGGC CATGCAAAAC CTGGGCCGAC TGGGTCCCAA TGCACCCGAC 
GATCTCAAAT CAGTGCCAAA TGCAAGTGGT CGCCCCTTGC CTCCGACGGG TCAGTCGCTC 
GACTACGGAC GCACTCCGCA CGATCACCGC AGCTTCGGAG GAGCCAACGA TCTGAACCGA 
CAGAGCAGTC CGGGCAGACC GCACTACCAT GACATGAATG CGTCCCGTAA TATTGATCCA 
CGCAATGGCA CTCCACAGCG TCCTTCGAAT CTGGGCCTAG AAAGCAGTCC ACGCAAGCCT 
TTGGTGGAGA CGAAGACGGA TTACGGCAAA TACAGTCGAA ACAACTCCGT ATCGCAAGCG 
GATTACACCA AGCTACCGAA GACGGCGCCA CATGGAGTGG TACCGCCGCC GAATGTGAGC 
AACGGCCAAA GCCAGATGAA TGGCAGTGGA ACGCCCAGCA GCAATGGCAG TGGACCCTTC 
AAGCCGGTGC CGCCACCCAA ACCCAAGAAC TATAGGCCAC CGGTGCAAAG TGGTGGCAGC 
AGTGGCAGCG GTGGCACTAC TCCGTGGGAA AATGGGGACT CTGGCTCGCC ACGCTCTCCA 
AATGGCTTCT ATTATCCACC AACGCCTTCG CATCATCACT ACGGCCAGCA GGCGACTCCC 
GGTTCACCCA GCAACGGCCA CATGCAGCCA CCTCCGCCGC AGCAGCAGCA GCAGCAGCCC 
ACCTACGGCG GAAGTAATGG CAACTACGGA CAGGCGCCAC CACCACAACC CTATCCGCAG 
GCCAATGGCT ACAACGGGAA TGGCCATCAC TACAACGGAG GCAGCGGCAC GGGACCCTAC 
ATTGCACCAC ATCGCGGCAT GCCGCCGCCA ATAGGAAACC TGCCGCCCCA CACGCCCGAA 
CGTCATGCCC TGGACTTGGC CGGAAGTCGG GAGCAGCGTG GCTCGGCCTT CGAGCTGTAT 
CGTAAGCCGC AAATCGGAGC AACCGCTGGG CACCATCACA ACATGAGCGA AATGGAGCCG 
TACGACGAGC GATACGATGA TTACTATAAC ATGCCACCCC CTGCCGCCCA TCCATCCCAA 
GGGCACCATA TGCAGCGCTC CCGATCAGCT CCTCGTTATC CCCAGGAGCG ACCTCCTCAC 
GCACAAGATC CGAATTACTA CGGCCACTAT GGAACTTCTA GGGGACATAG TCAGCCGCGC 
CAGCAGTATC CTCAGCATCA CCAGCAACAG TATTACGATG AGCATGGCAT GGAGATGGGT 
CCACCGCCAC TGCCCCCACA TAAGAAGAAG AAATCGGTGC TGAAGAGCCC ACTGGTGGCC 
CTCAAGAATG CGCTGCTTAA GAGCACGCGG CCCCTGAGGA GAATGAACAG CATGGTGGAG 
CCGGAGAGGA AACCGAAGGG ACTCCGACGC CAGCAATCGA TGCTGGAGAG GGGTGTGCAA 
AGACCCTACT ATCCGGATGA GTATCCAACC TATCCAGCTG GCTTTGAGGA GCGTGCCCAT 
GGCGGACAAG GCATGATGCA GCCTCATCCG CAAGATGCCT ACTATCAAAG GGGTGGTCAC 
TACACGTCGC AGCAGCAGGA GATGATGAAC AGCACCTACC AGAATCTGGA GGGTGAGGAT 
ATCTATGGGA ACATCGGAAA CACAGTTCCT AGAATGCCGC ATCACCAGGA CAATGGTTAC 
GGATATGACC AATACGATCT CTATGCCAAT CGAGCGTGCA TCGATCTCGA AAGAAGGCAA 
GCAGAGGCAG CTGCAGCTTC CGGTGGCAGA AATGGGAGGA GAATTGTGAG GCGGCACAGC 
ACCACCACTG CGGATCGAGG AGGAAATCCC GGACCGCCCA GGAGGCCTAT GATCAGTCCT 
GGTTATGAGC AGGATCCGCA AGAGATCTAC CAGACCCGCA ACGGAGCCTA CATGCTGCCC 
GATCAGCGAA GGGCGCCCAG TTCGGAGGTG ATGACCCGCA GAAGATTCTA TCCGGGGGCT 
GCCAATGAGC AAACTGAGGA GGAGCCCCTC TACCAATCCC GTCGCGAAAT GCAGCGTGAG 
ATGCAGAGGA ATCATCTGTA CCAATCGAAA CGGGAAATGC AGGAGAGGAT TAGCCAGGGC 
AAGAGGGATA TGGAGCGAGA GTTTAGCCCC CAGGGCAGCA GCAGCCAGTC GGAGACCTCC 
AATCCGGAGG TAATATACCA GAGTCGCCGG GAGGCCAAGG AAAGTGCTCT GAAAACGAGG 
GCACAACTGA GGGATCAGAT CTATCAGACT AGACGGGAAG CCCTGGACAG CATGGCGGAA 
CCGATCTATG TGTCCAAAAG AGATATGGGC AGACCGGCTC CTATCTATGA GACTCGGGAA 
GAGAGTATCC TCCAGTCCCG AGAAAATGAA ACCGACGAAA AGAAGGAAAA GGAGTGCAAG 
ACCGAGCAGA TTCAGGTGGA GATTAATGCT CAACCGGAGG AACAGGTGGA AGATTCGACC 
TTAAGTCGAT CCGACTTGCA GAAATCTTCG GACACGGTGA TAGAAAATCC AGCGAGAGCT 
CCTCTTGTGC AGGATGAGGT AGATGACGAC GAAGAAGAGC AGGCTCAGGC TCGGGATGAT 
CCCGCTGAAA GCTCTGCGGA TCAGACAGAG CTGCCAACGG CCATTGAAAA CAATCTACAC 
AACGAGGGAA TGGTGGAGCA AACACAGAAT GAGTCGGATG ACGTATTCGA AGCAGCCGAT 
AAGGTTTCTC CTCTAGCCGC TCCTACAGGT ACTGTTCAAC CGCCGACCCC GCGATCCAGT 
AGAGCTCCCT TCCACATTTC GAATATCCTA AAGCGAACAG CACCTCCTCC GAGTTCCCCA 
ATGGGCGACA GCTGCACCTC CATCGAAACC CAGTACACAT CCCAGGCCAG CCTGCCAGTG 
GGCCCACCAA ATGCCACCAG TACGCCGTTC AGTAGCAGCA TGTCATTGCC CATCGCCGGA 
CCCGTAAACA ATCAACCGCC GGCCAGCGGA CCCTTTCCCA CTCTGCCCCG CGAACCCAGC 
ACCTCGCGCG GATTCTTTGA CTCCAATGGT GGCACTCTGG CAGATAAGCT GTGGCACGTC 
TCACTGCAAA TCCCGCCGGG AGCCATTCCA GCCGGAGTAC GGCAAGAGAT CTACTTCACC 
GTGAGCGATC CGCGAATGGG ACAAGCCGTC GGCGGTCCTC CGCTGGACAT GGAAAATGGT 
GAGACAATGC TGTCACCGCT GGTGATGTGC GGGCCGCAGG GCCTTGAGTT CCTGGTACCG 
GTTACACTCA ACATACCGCA CTGCGCCGGA CGAACTGCAT CACTGGGACT GGCCCTCAAG 
GCGACCGACA GCGAGAAGAA CCTGCACACC GAGTGGGACA ACATCGACCT GCCCAGCAAT 
GCCGCCGCCC ACACGGTCTC CGTCAAGGTG GATCACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015010031.1
CDS	142..7503
Translation	MTREEEEEVRSLLSRHQERIMLDLGSTDLLAVLVKNSVLSQSEEDLLLKPYNAVTPTPSAAASATVEPPGSAPSLTKRKLSAVASSGSTASGGSAGSGGSGSSRCASVNGDANCDSRSLTPSGGGPCSVLNDAEILRVQCSNLIEIIAKNGFEKFKQFCYAIECECPQLIEDLINDRLKTDAANLEDSRDGEKMQKEIETIDYIDKEKENDRNRRAVSYGGDVTSSPHSATIPRAAPRRVALMKDPPPPPPPKPQLGSKADSVAHLASKYPQSEYNLIQKIDSNSTLTAPAQYQPQNFYANTGTISSTNGYGSLLCHASSTTSPLAVRKRDKLLHRFSDAATLGRKLKKKKNTNRTCRSMTEAIEMLADPVIEDEFFGDRTTWEYHTVAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEYATAVQVLRDSGNTVQLVVKRRVPLNPINNGGAVQHQHSHSLSSVGLLANGSGGVAPTPLTSLSQPNSLNSSLVQNASSGQPIKVTLTKGGKKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQAAVSQLNLNNSASHQASANIYGTHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNLNGNGLNDEKSNLTPRGRSRGPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQFYPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQTQKLERVWSHIFSTQIALSDEESWYRKLRDSIDLQQSGAVWMSESKVPYDHGVPANPNRRQTMDSSKYSIYGTNVPPQPQQPGAGGDPAAVRPQSLYGINAPDLPPRIDRQSKPGDMPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQTHASLERQARLNAQLKANGGGAGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNASGRPLPPTGQSLDYGRTPHDHRSFGGANDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLVETKTDYGKYSRNNSVSQADYTKLPKTAPHGVVPPPNVSNGQSQMNGSGTPSSNGSGPFKPVPPPKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSHHHYGQQATPGSPSNGHMQPPPPQQQQQQPTYGGSNGNYGQAPPPQPYPQANGYNGNGHHYNGGSGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEMEPYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPQERPPHAQDPNYYGHYGTSRGHSQPRQQYPQHHQQQYYDEHGMEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLRRQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHPQDAYYQRGGHYTSQQQEMMNSTYQNLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRHSTTTADRGGNPGPPRRPMISPGYEQDPQEIYQTRNGAYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQSKREMQERISQGKRDMEREFSPQGSSSQSETSNPEVIYQSRREAKESALKTRAQLRDQIYQTRREALDSMAEPIYVSKRDMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQVEDSTLSRSDLQKSSDTVIENPARAPLVQDEVDDDEEEQAQARDDPAESSADQTELPTAIENNLHNEGMVEQTQNESDDVFEAADKVSPLAAPTGTVQPPTPRSSRAPFHISNILKRTAPPPSSPMGDSCTSIETQYTSQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPPASGPFPTLPREPSTSRGFFDSNGGTLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLEFLVPVTLNIPHCAGRTASLGLALKATDSEKNLHTEWDNIDLPSNAAAHTVSVKVDHF

Target ORF information:

RefSeq Version	XM_015154545.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant H (Dere\GG17413), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015154545.1

ORF Insert Sequence:

ATGACGCGCG AGGAAGAGGA AGAGGTGCGC TCCCTGCTCT CCCGTCACCA GGAGCGTATT 
ATGCTGGACC TGGGGAGTAC CGATCTATTG GCGGTTCTGG TCAAGAACTC GGTGCTCAGT 
CAATCGGAAG AGGATCTCCT ATTGAAGCCC TACAACGCAG TAACGCCCAC GCCCTCTGCT 
GCCGCCTCTG CCACAGTGGA GCCTCCCGGC AGTGCACCCT CCCTGACCAA GAGGAAACTA 
AGTGCCGTCG CTAGTAGTGG GAGTACTGCT AGCGGAGGGA GTGCCGGCAG CGGAGGCAGT 
GGATCCTCCA GGTGCGCCAG CGTAAACGGA GACGCCAACT GCGATAGTCG CTCACTGACG 
CCCTCTGGCG GGGGGCCATG CTCGGTTCTA AACGACGCCG AAATCCTGCG AGTCCAGTGC 
TCCAATCTCA TCGAAATCAT TGCCAAGAAC GGATTCGAGA AGTTCAAGCA GTTCTGTTAC 
GCCATCGAGT GCGAGTGTCC GCAGCTCATC GAGGATCTAA TTAACGATCG TCTCAAGACC 
GATGCCGCCA ATTTGGAGGA CAGCCGCGAT GGGGAAAAAA TGCAAAAGGA AATCGAAACG 
ATCGATTACA TTGACAAGGA AAAGGAAAAC GATCGCAATC GTCGGGCTGT TAGTTATGGC 
GGTGACGTCA CCTCCTCCCC CCACTCGGCG ACCATACCCC GCGCCGCCCC CCGTCGTGTG 
GCCTTGATGA AGGATCCACC GCCACCGCCG CCACCCAAGC CGCAATTGGG CTCCAAAGCG 
GATTCCGTAG CGCATTTGGC CAGCAAATAT CCCCAATCCG AGTACAATCT CATACAGAAA 
ATCGACTCGA ACAGCACACT CACTGCTCCG GCGCAATATC AACCGCAGAA TTTCTACGCG 
AATACCGGAA CCATATCCTC CACGAATGGC TATGGATCGC TGTTGTGTCA CGCCAGTTCG 
ACGACCAGTC CGCTGGCGGT TCGGAAGCGG GACAAGCTGC TCCATCGGTT CAGCGATGCC 
GCCACCTTGG GCCGGAAGCT CAAGAAGAAG AAGAACACGA ATCGCACCTG CAGATCCATG 
ACGGAGGCTA TTGAGATGCT GGCCGATCCC GTCATCGAGG ATGAATTCTT TGGTGATCGA 
ACCACTTGGG AGTACCACAC GGTGGCAGTG ACTCGGGTGC CGGGCTATGG ATTCGGCATC 
GCCGTTTCCG GTGGACGTGA TAATCCGCAC TTCGCCAACG GCGATCCCTC GATAGCAGTT 
AGCGATGTGC TGAAAGGTGG CCCCGCCGAG GATCGATTGC AAGTCAACGA TCGGATAATC 
TCGGTGAACG GCGTCTCCCT GGAGAACGTG GAATATGCCA CCGCGGTGCA GGTGTTGCGC 
GATAGTGGCA ACACGGTCCA ATTGGTGGTC AAGCGTCGCG TGCCCCTCAA TCCCATCAAT 
AATGGAGGTG CAGTGCAGCA TCAGCACTCG CACAGTCTCA GCTCGGTTGG TCTATTGGCC 
AATGGAAGTG GAGGAGTTGC ACCAACGCCC TTGACGAGCC TGAGTCAACC CAACTCCCTG 
AACTCTTCGC TGGTGCAGAA TGCCAGCAGT GGTCAGCCCA TCAAGGTGAC CCTCACGAAA 
GGCGGCAAGA AAGATGACTA TGGCGTGGTC CTTGGCTGCC GGCTGTTTGT TAAGGAGATC 
TCCTCCAAAG CTCGCGAGCA ACTTAATGCC AATGGCTACA GTTTGCAAGA GGGTGACATT 
ATCACCCGCA TCCACAACAC CAATTGCGGG GATACGATGA GTCTCAAGGA GGCGAAAAAG 
ATCATCGATG GTTGCAAGGA GCGCTTGAAC CTAGTGGTTC TACGGGACAT CACCAACCAA 
GCAGCTGTTA GCCAACTGAA TCTGAACAAT TCAGCGAGCC ACCAGGCTTC GGCAAATATA 
TACGGCACTC ATCAGCCTCA GGTATCCGGA TGTAGCAGCA GTAACAACAA TCTTGAAGAT 
CCATATCTGC CGGGCGGAGC CAGTTACTCC TCGCAGAATC TGTACGTGCA ACCACCCACT 
CGCACCTCCA ATGGTCCCAA TCTCAATGGC AATGGGCTGA ACGACGAAAA GAGTAATCTC 
ACACCGCGAG GTCGCTCCCG GGGGCCCATT ATGGATGGGG TATCTCTGCA GCAGCTGGAC 
AGGCCCGTAA CACCGACGCG TGGCAGGAGT GCGGCCATCG ACGAGCCGCC ACGTCCACCG 
CCGCCACGAG GATCGAGCGG GGGAGCGGCC CAGGAGGATT TCTACAGCTC TCGCAGGCAA 
TTGTACGAGG AGCGCCAGAG TGCCGAGCCG AGATTCATTT CCTTCCAGAA GGAGGGAAGC 
GTGGGCATCC GCCTAACCGG CGGCAATGAA GCCGGTATTT TTGTGACTGC CGTACAACCG 
GGATCTCCAG CCTCGCTGCA GGGTCTAATG CCAGGCGATA AGATCCTGAA GGTCAACGAC 
ATGGACATGA ACGGAGTGAC GCGCGAGGAG GCTGTTCTCT TTCTGTTGAG CCTGCAGGAT 
CGCATCGATC TGATTGTGCA GTACTGCAAG GAGGAGTACG ACGAGGTGGT GACCAACCAA 
CGTGGCGACT CCTTCCACAT CAAGACACAT TTCCACTGCG ATAATCCCTC CAAAGGCGAG 
ATGGCCTTCA AGGCGGGCGA TGTGTTCCGG GTGATCGATA CCCTGCACAA TGGTGTAGTC 
GGTTCCTGGC AGGTGCTGAA GATCGGACGT GGTCACCAGG AGATGCAGCG CGGCGTTATA 
CCGAACAAGT CGCGGGCCGA AGAACTGGCC ACTGCCCAGT TCAATGCCAC CAAGAAGGAG 
ATGAACGCCA ACGAGTCGAG GGGCAACTTC TTCCGGCGAC GACGCTCGAC GCACCGACGC 
TCGAAGAGTC TGTCCCGCGA GAACTGGGAC GACGTGGTCT TCTCCGACAG CATTTCCAAA 
TTCCCCGCCT ACGAGCGAGT CGTTCTCCGC CATCCTGGCT TTGTTCGACC CGTAGTGCTC 
TTCGGACCCG TATCCGATCT GGCCCGCGAG CGCTTGGCCA AGGATTTCCC CGACAAGTTT 
TCGACTCCAC TGCAGGATGA TGACAAGTCA GCTGCAACCA GTGGAAAGTG CCGCATCGTA 
AGGCTCTCGA ACATACGCGA TGTGATGGAT CGTGGCAAGC ACGCCCTGCT GGACATAACG 
CCCAATGCAG TGGATCGTCT CAACTACGCC CAGTTCTATC CGGTGGTGAT CTTCCTGAAG 
ACAGACAGCA AGCACGTGAT CAAGCAGCTG CGCCACGGAC TACCCAAGGC GGCCCACAAG 
AGCTCCAAGA AGCTGCTGGA GCAGACCCAG AAACTGGAGC GCGTCTGGTC CCACATCTTT 
AGCACCCAGA TCGCGTTGAG CGACGAGGAG TCCTGGTACC GAAAGTTGCG CGACTCGATC 
GATCTGCAGC AGAGCGGCGC CGTGTGGATG TCCGAGTCCA AGGTACCCTA CGACCATGGT 
GTCCCCGCGA ATCCCAATCG CCGCCAGACC ATGGACTCCA GCAAGTACAG CATCTACGGC 
ACCAACGTGC CGCCGCAGCC GCAGCAGCCC GGCGCAGGAG GAGATCCGGC TGCAGTGAGG 
CCACAGTCTC TGTACGGGAT TAATGCACCC GATCTGCCGC CCCGCATCGA TCGTCAGTCC 
AAGCCGGGTG ATATGCCACT GAACACCTCG GGCTCGTCGT CGCGAAATGG CACCCTGGGT 
CGCAGTGCCC AGGAGCGTCT GTTTGGCAAG GCTGTGGTGC AGGACGATGT CCAGGCGGAA 
TACATAACCC GAAATGCTCT GGTCGGCTCC GGGGCGGCTG AAACCCTGGA TCGCCAGCAG 
CAGCAGCAAA CCCACGCGTC CTTGGAGCGG CAGGCGCGTT TGAATGCGCA GCTGAAGGCC 
AACGGAGGAG GCGCCGGAGG AGGTGCATCC ACCTACGACA GTGTGTCCAG CTACGACTCG 
TACAACAACA CACAAATGGC CATGCAAAAC CTGGGCCGAC TGGGTCCCAA TGCACCCGAC 
GATCTCAAAT CAGTGCCAAA TGCAAGTGGT CGCCCCTTGC CTCCGACGGG TCAGTCGCTC 
GACTACGGAC GCACTCCGCA CGATCACCGC AGCTTCGGAG GAGCCAACGA TCTGAACCGA 
CAGAGCAGTC CGGGCAGACC GCACTACCAT GACATGAATG CGTCCCGTAA TATTGATCCA 
CGCAATGGCA CTCCACAGCG TCCTTCGAAT CTGGGCCTAG AAAGCAGTCC ACGCAAGCCT 
TTGGTGGAGA CGAAGACGGA TTACGGCAAA TACAGTCGAA ACAACTCCGT ATCGCAAGCG 
GATTACACCA AGCTACCGAA GACGGCGCCA CATGGAGTGG TACCGCCGCC GAATGTGAGC 
AACGGCCAAA GCCAGATGAA TGGCAGTGGA ACGCCCAGCA GCAATGGCAG TGGACCCTTC 
AAGCCGGTGC CGCCACCCAA ACCCAAGAAC TATAGGCCAC CGGTGCAAAG TGGTGGCAGC 
AGTGGCAGCG GTGGCACTAC TCCGTGGGAA AATGGGGACT CTGGCTCGCC ACGCTCTCCA 
AATGGCTTCT ATTATCCACC AACGCCTTCG CATCATCACT ACGGCCAGCA GGCGACTCCC 
GGTTCACCCA GCAACGGCCA CATGCAGCCA CCTCCGCCGC AGCAGCAGCA GCAGCAGCCC 
ACCTACGGCG GAAGTAATGG CAACTACGGA CAGGCGCCAC CACCACAACC CTATCCGCAG 
GCCAATGGCT ACAACGGGAA TGGCCATCAC TACAACGGAG GCAGCGGCAC GGGACCCTAC 
ATTGCACCAC ATCGCGGCAT GCCGCCGCCA ATAGGAAACC TGCCGCCCCA CACGCCCGAA 
CGTCATGCCC TGGACTTGGC CGGAAGTCGG GAGCAGCGTG GCTCGGCCTT CGAGCTGTAT 
CGTAAGCCGC AAATCGGAGC AACCGCTGGG CACCATCACA ACATGAGCGA AATGGAGCCG 
TACGACGAGC GATACGATGA TTACTATAAC ATGCCACCCC CTGCCGCCCA TCCATCCCAA 
GGGCACCATA TGCAGCGCTC CCGATCAGCT CCTCGTTATC CCCAGGAGCG ACCTCCTCAC 
GCACAAGATC CGAATTACTA CGGCCACTAT GGAACTTCTA GGGGACATAG TCAGCCGCGC 
CAGCAGTATC CTCAGCATCA CCAGCAACAG TATTACGATG AGCATGGCAT GGAGATGGGT 
CCACCGCCAC TGCCCCCACA TAAGAAGAAG AAATCGGTGC TGAAGAGCCC ACTGGTGGCC 
CTCAAGAATG CGCTGCTTAA GAGCACGCGG CCCCTGAGGA GAATGAACAG CATGGTGGAG 
CCGGAGAGGA AACCGAAGGG ACTCCGACGC CAGCAATCGA TGCTGGAGAG GGGTGTGCAA 
AGACCCTACT ATCCGGATGA GTATCCAACC TATCCAGCTG GCTTTGAGGA GCGTGCCCAT 
GGCGGACAAG GCATGATGCA GCCTCATCCG CAAGATGCCT ACTATCAAAG GGGTGGTCAC 
TACACGTCGC AGCAGCAGGA GATGATGAAC AGCACCTACC AGAATCTGGA GGGTGAGGAT 
ATCTATGGGA ACATCGGAAA CACAGTTCCT AGAATGCCGC ATCACCAGGA CAATGGTTAC 
GGATATGACC AATACGATCT CTATGCCAAT CGAGCGTGCA TCGATCTCGA AAGAAGGCAA 
GCAGAGGCAG CTGCAGCTTC CGGTGGCAGA AATGGGAGGA GAATTGTGAG GCGGCACAGC 
ACCACCACTG CGGATCGAGG AGGAAATCCC GGACCGCCCA GGAGGCCTAT GATCAGTCCT 
GGTTATGAGC AGGATCCGCA AGAGATCTAC CAGACCCGCA ACGGAGCCTA CATGCTGCCC 
GATCAGCGAA GGGCGCCCAG TTCGGAGGTG ATGACCCGCA GAAGATTCTA TCCGGGGGCT 
GCCAATGAGC AAACTGAGGA GGAGCCCCTC TACCAATCCC GTCGCGAAAT GCAGCGTGAG 
ATGCAGAGGA ATCATCTGTA CCAATCGAAA CGGGAAATGC AGGAGAGGAT TAGCCAGGGC 
AAGAGGGATA TGGAGCGAGA GTTTAGCCCC CAGGGCAGCA GCAGCCAGTC GGAGACCTCC 
AATCCGGAGG TAATATACCA GAGTCGCCGG GAGGCCAAGG AAAGTGCTCT GAAAACGAGG 
GCACAACTGA GGGATCAGAT CTATCAGACT AGACGGGAAG CCCTGGACAG CATGGCGGAA 
CCGATCTATG TGTCCAAAAG AGATATGGGC AGACCGGCTC CTATCTATGA GACTCGGGAA 
GAGAGTATCC TCCAGTCCCG AGAAAATGAA ACCGACGAAA AGAAGGAAAA GGAGTGCAAG 
ACCGAGCAGA TTCAGGTGGA GATTAATGCT CAACCGGAGG AACAGGTGGA AGATTCGACC 
TTAAGTCGAT CCGACTTGCA GAAATCTTCG GACACGGTGA TAGAAAATCC AGCGAGAGCT 
CCTCTTGTGC AGGATGAGGT AGATGACGAC GAAGAAGAGC AGGCTCAGGC TCGGGATGAT 
CCCGCTGAAA GCTCTGCGGA TCAGACAGAG CTGCCAACGG CCATTGAAAA CAATCTACAC 
AACGAGGGAA TGGTGGAGCA AACACAGAAT GAGTCGGATG ACGTATTCGA AGCAGCCGAT 
AAGGTTTCTC CTCTAGCCGC TCCTACAGGT ACTGTTCAAC CGCCGACCCC GCGATCCAGT 
AGAGCTCCCT TCCACATTTC GAATATCCTA AAGCGAACAG CACCTCCTCC GAGTTCCCCA 
ATGGGCGACA GCTGCACCTC CATCGAAACC CAGTACACAT CCCAGGCCAG CCTGCCAGTG 
GGCCCACCAA ATGCCACCAG TACGCCGTTC AGTAGCAGCA TGTCATTGCC CATCGCCGGA 
CCCGTAAACA ATCAACCGCC GGCCAGCGGA CCCTTTCCCA CTCTGCCCCG CGAACCCAGC 
ACCTCGCGCG GATTCTTTGA CTCCAATGGT GGCACTCTGG CAGATAAGCT GTGGCACGTC 
TCACTGCAAA TCCCGCCGGG AGCCATTCCA GCCGGAGTAC GGCAAGAGAT CTACTTCACC 
GTGAGCGATC CGCGAATGGG ACAAGCCGTC GGCGGTCCTC CGCTGGACAT GGAAAATGGT 
GAGACAATGC TGTCACCGCT GGTGATGTGC GGGCCGCAGG GCCTTGAGTT CCTGGTACCG 
GTTACACTCA ACATACCGCA CTGCGCCGGA CGAACTGCAT CACTGGGACT GGCCCTCAAG 
GCGACCGACA GCGAGAAGAA CCTGCACACC GAGTGGGACA ACATCGACCT GCCCAGCAAT 
GCCGCCGCCC ACACGGTCTC CGTCAAGGTG GATCACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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gene@genscript.com

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1-877-436-7274

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ONLINE FORM

Online Quote Submission

LOC6552588 cDNA ORF clone, Drosophila erecta

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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