Dana\GF22254 cDNA ORF clone, Drosophila ananassae

Gene
Clones
gRNAs

Summary

Gene Symbol	Dana\GF22254
Entrez Gene ID	6504918
Full Name	GF22254 gene product from transcript GF22254-RB
Synonyms	GF22254,dana_GLEANR_6229
Gene Type	protein-coding
Organism	Drosophila ananassae

mRNA and Protein(s)

mRNA	Protein	Name
XM_014904369.1	XP_014759855.1	uncharacterized protein, isoform B
XM_001966553.2	XP_001966589.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dana\GF22254 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dana\GF22254 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODh13535	XM_001966553.2 Latest version!	Drosophila ananassae uncharacterized protein, transcript variant A (Dana\GF22254), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODh15777	XM_014904369.1 Latest version!	Drosophila ananassae uncharacterized protein, transcript variant B (Dana\GF22254), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dana\GF22254 ( XM_001966553.2 ) cDNA ORF clone, Drosophila ananassae -> XP_001966589.1 Drosophila ananassae uncharacterized protein, transcript variant A (Dana\GF22254), mRNA.

Price & Availability↑

CloneID	ODh13535
Clone ID Related Accession (Same CDS sequence)	XM_001966553.2
Accession Version	XM_001966553.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450108800000
Organism	Drosophila ananassae
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001939307). On Dec 16, 2015 this sequence version replaced XM_001966553.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCGG GCGTTCTGGC CAACGATAGC CGGGCCAACT CCACCGATGA CATCCAGATC 
GCCCTCGCCC CCCACATCCA GACCTACCTG AGCCAAACGG GACGCCGGCA CTCCTGCTGC 
AGTGTCATGC TGCCGGTGGC GTTCGAGCGG GCGGCGGCCA AGTCCTGGCT GGATCCCAAG 
TTCGACTCGC CCGTGCTCGA GGAGCAGTAC CAGACCAGTG TCTATCCCCA TGTCCGAATG 
CGTTACAGAT TCACTTTGTC GTACATTTTG CTCTGCTCCC TGATGTGGTG TTTGTACTTC 
GTGGTGGACG GCGGATCGGA GGACTTCTGG CGGCCCATCT CCAGCTCCTT CTCGATGCTC 
TCCCTGGTGA CGATCATGGC GCTGTGCTTC ACCCACTGGG ACCTGTACAG GGAGCACCGG 
ACAGTGACCT CGGCGGTGAC GGCGCTGCTC CTCTGCGGCG CCTCCCTGGC CTTCCTGACG 
TACACGGGCC GGGCGTTCAG TCCCCTGGGC CACTTTGCCA TATGCCTGGA GATCGTGCTG 
CTGATCTACA CGGCCCTGCC GATGCCCCTG TGGCTGGGAG CCGGCATAGC CATCAGTTAC 
TCCATAGCCT TCGAGATGGT CTCCCACATG GTGATCGGAT GCAGTGCCAT CCATGGCAGT 
GGCTCGACCG AAGGTGTCAC CGGATCAGTG CCCGCCAACG GGGATCCCAG CAACAAGATC 
CTAATACTCC GAATCATGGC CCATTTGAGT GTCCACCTGG TGGGGGTCCA TATCCTGGTG 
ATGAATCTGG TCCGGATGCG AGGCACCTTC ATGAAGGTCG GCCAGAATCT GCTGGTGCGC 
CGCCAACTGG AGATGGAGAA GCAGCTGAAG GAGAAGATGA TACACTCGGT GATGCCCCCG 
AAAGTGGCGG ACATGCTGCT GAACGAGGGC GGCACCTCCG GCCTGGATGC CACCGGAATG 
CCGCCCGAAT CCCACTACAT GCGTCCCCGG GCCTCCAACG ATGTGAAGTC CCTGTTCCGG 
CCCTTCCACA TGCACAGCAT GGAGAATGTC AGCATCCTGT TTGCGGACAT CGTCGGCTTC 
ACTCGCATGT CCTCCACGAA GACCGCCGAG CAGCTGGTCG AGATCCTCAA CGATCTCTTC 
GAGCGCTTCG ACGACCTCTG CACGCTGAGT GGCTGCGAGA AGATCTCCAC CCTGGGCGAC 
TGCTACTACT GTGTCTCCGG ATGCCCGGAG CCCCGGGCGG ATCATGCCAT CTGCTGTGTG 
GAGATGGGTC TGGGCATGAT CGATGCGATG CGCTGTTTCG ACGCCCAGCG GCACGAGGGC 
GTCAAGATGC GGGTTGGCGT CCATACGGGC ACAGTCCTGT GCGGCATTGT GGGCACCCGG 
CGGGTCAAGT TCGATGTCTG GAGCAATGAC GTTAGTTTGG CCAACAAAAT GGAATCCTCT 
GGCAAACCGG AACAAGTCCA CATCTCCCAG GAGACGTCCA GCTTTCTGGG GGATGCCTAC 
TACCTGGAAG AGGGCGAGGA AGTTTTCGGA CACCGTACTT ACTTCGTGGT GGGTCGTCGT 
CGGGACTTCT CCCGCACCAA TAGCCTGAGT CCCAGCATGC CGCCGAACCC GGGCGGCAGC 
TCCCTGCTCC TGCCCGGCGT CCATGCCAGC TTGTCGCAGA GCGCCACCAA TGTCTCGGTG 
GTGCAGCCGC ACGTCCCGCC CGCCTCGCCG GTGGGCCAGC TCTCCAGCTC CCTGAATCCA 
TCCCCAGTGC TCTCCATGCG CCCCCGGCTC ACCTCCCTGA GCATGAAGCT GCGCAAGAAG 
TCGCAGAGTC GGGAGCGGGA GAGGGAGAGG GACGTGGAGC GGGGCATCAT CCATCCGGCG 
GCGGCGGGAG TTCCGCCAGT GATTGTGGTG CGGGAGCGAC CCAAGATCAT CATCACCACC 
AAGTCCCTGC CCGGCAGCCT GGACTCGGAC GAGCAGCCGC CCAGTAGTCC GCCCATCAAG 
GCGCCGCCCG CCGCCCCCCA GCCGCCGGCC GAGTCGCGTC CAAAGATCCG ATTGAAGATC 
TGGAACCGAG TGCCGAGATT CCTGAAGCGG CGCAACAGCT CCTACGACTC GGAGGAGAAC 
AGGGAGAAGG TCCACGGCCA GGATGTGGAG AAGGGCATCG ACCGGGAGAG GGAGAAGGAG 
CAGGAGCGCG AGGATCAGGC CCTGAATCCG GAGGAGACGC TGTCCTTCAT GGATAACCAG 
ATCCAGAACG GCAACGGATG CGGCTACCAG CAGCTGCCCG TCCTGGTGGA GGCCACCACG 
AATCGCGTCC AGACTCTGGA CATCCCGCCG GCCCGGCCAG TCCTCCATCA CGCCGCCACC 
TCGACGGCCC TTGCCAGCAG TGTCCTGCGA TCCCCGGAGC CTAGCACCGG CGGATGCTGC 
TCGCCGGGCC AGTACTCCAT GTACGACGAC ATCATCGACG TGAGGTCCTA CATCAGCCAG 
TCGAGGAGCG ACATCTCGCC GTTCGGCCGG TCTGGGAGCT ACCGATCCCA GTGCGGCAGG 
CAGTCCATGG GCGGAGGAGC ACCTCTAGTC GGAGCACCGG TGGCGGGAAC GTCGGGCGCA 
GCCCCGGGAG TATCCACGCA GGTGACACCA TCGTCCTGTG TGGAGCAGTC CCCGTTGCCA 
CGTCCCCGGG CCTCGACACT GGCCACTGGA CGGCCCGGAG CCAGCCTGGA GCCCGGAACA 
TCACAGAATC CCAGTCCCAG TTGCCTGCCA GCGCCGGGCG GACCCGGAGT ACCCGGTCTG 
TCCGGCTTCG GGGGCATGTT TCCGCCCACC CACTCGCGGC AGAGCAGCAT CTGCCCGTCG 
GCCACCTCGC GGAAGGACTC GGGCATCAAG AGCAACTCCC GCCGGTCCTC CATCCAGCAG 
CAGATCTACG TCCTGAACCA GTCGGCCATT AGCCAGCACA GGGTGTCCGG ATACTTCACC 
AGTTCCACCT CCAGCATCTC GAATTTGGGC GAGGTCCAGG GCATGGCTCC GGGGGCCATG 
GCGGGAGTGG CCGCCCCACC GCCACCCCTC CTGATGAACG CCTGCTCCTC GCAGATAGCC 
GATCCGCTGG CGGCCTGCCT CCAGCAGCTG AGGAAGCAAT CGGACCTGCA GCTCATCCGA 
TGCGTCCGGG ACAATGCCAG GTCGCAGAGG AGCTACCTGG TGAAGCCGCC GCTCCGGGGC 
TTCAGCCTGT ACTTCAAGTC CCGGCAGCTG GAGCGGGACT TCCGGTCGAA GGCCCACCGC 
TTCGGGGCCG AGAACGAAAC GGAAGGACCT CCCACCCTGG CCACGCCCCG CTACAACACC 
TACATAGACA TCTTCGTGGG CATCGCCGTC TACCTGTGCA TCTCGATATC CCTCTTCCTG 
ATGACCCAGA ACACGGTGAG CCCCAGCTTC CGGCTGTGGG TGACACTGTT CACCTGCTTC 
ACGGGCATCC AGGTGTTCGC TCTGTTCCTC TTCACCCGCC AGATGTGCCG GCGGCAGAGT 
GGGTCGAGTG CCGGCAGTGC CAACCACCGA TTGCGCTCCA AGTCCACCAC CAGCGATGAT 
GTGGAGGCCA GGCGCCAGAC CGAGGATAGG GGCGAGACAC CAGCTCCCCA GCAGTTCGAG 
TCCTGTGCCG ATCGCATCTT TGAGGCCATC TCCAGCTGGT ATCCGTGGCA CATCTGCCTG 
GCCATCCTGA TGGCGATGCC CGTCCTGCTG ATTATCGCCA ACTTCCTGCT GCTGGACCTG 
GAGCAGCTGG AGGCCTTCGA ATACCACTAC GGCTTCCTGA TCTTCGTCTG CATCGTCCAC 
TTCTGCAACT TCACCCAGCT GAACTGCTGG GTGCGGAATG TCCTGGCCTT CCTGGCGGCC 
CTCTGCTTCA TCGCCATCGC CATGTCCCAG CTGATGGTGT ACGACAGTCG GGTGGAGGAC 
CAGCAGGACG ACAGGGGCGC CAACTTCATT GAGGAGATCA AGTGGTTCCA GGACTACCAC 
GTCGAGATCT ACTTGGACCT GTTGCTCATC CTGGTGCTGG TGTGGTTCCT CAATCGCGAA 
TTCGAGATTG GGTATCGGCT GACCTTCTAC GGCAATGCCG TCGCCAACCA GGACAAGGTC 
CGCGTCCAAA ACATGAAGAA CCAGGCGGAC ATGCTGCTCC ACAACATCAT ACCCAAGCAC 
GTGGCGGAGC ACCTGAAGAA CACGGCCAAG TACTCCGAGA ACCACCACAA CATAGCCATT 
ATATTCGCAT CGATAGTCAA TTTCAACGAG ATGTACGACG AGAGCTATCT GGGGGGGAAG 
GAGTTCCTCC GGGTCCTCAA CGAGCTGATC GGGGACTTTG ATGAGCTGCT CTCCCGGCCG 
GAGTTCCGGG CGGTGGAGAA GATCAAGACG ATCGGCTCCA CCTTCATGGC GGCCAGTGGC 
CTGGATCCTT CCCATCGGGG CACTGGCGAC GAGCACATCC ACACCCTGAT GGAGTTCTCC 
ATCGCCATGC AGGAGGTGGT GGACGCCTTC AACAAGGACC TCCTCGAGTT CAACCTAATC 
CTGAGGATCG GCATGAATAT CGGAGATGTT ACAGCCGGAG TGATCGGCAC CAGTAAGCTC 
TACTACGACA TCTGGGGGGA TGCGGTGAAT GTGGCCTCCC GGATGGACTC CACGGGCCTG 
CCCAATCGCA TCCAGGTGGG CAAGGACTGC CTGCCCTTCC TGCAGAGCCG CTACGAGTTC 
GAGCCCAGGG GCAGTGTGTA TGTGAAGGGC AAGGATCACA TGGAGGTGTT CCTCTACACA 
GGACGAATGG AGAAGCTGAC CGAAGAGGCG GAAGTTCCAG AGAAGCCAGA GAAGCGCGAA 
TCAGTCGTAT GGATGGAGGA TCTAACTAAC CCGGAAGACA ACGAGGACGA CGAGGAGGAG 
GAGGAGGAGG ATGATCTGCA CTCCAGTGAG ACCACCACCC TCTTCAAATC CCAGGAGTCA 
CTTCACGCCA ATGGTGTCTC CAATTCCCAT CCAAGTCCAA CTCTGAATCA GAATCAGAAT 
TCGAATCCGA ATCCAGCCAT GCCAGTGGAG ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001966589.1
CDS	141..5156
Translation	MPPGVLANDSRANSTDDIQIALAPHIQTYLSQTGRRHSCCSVMLPVAFERAAAKSWLDPKFDSPVLEEQYQTSVYPHVRMRYRFTLSYILLCSLMWCLYFVVDGGSEDFWRPISSSFSMLSLVTIMALCFTHWDLYREHRTVTSAVTALLLCGASLAFLTYTGRAFSPLGHFAICLEIVLLIYTALPMPLWLGAGIAISYSIAFEMVSHMVIGCSAIHGSGSTEGVTGSVPANGDPSNKILILRIMAHLSVHLVGVHILVMNLVRMRGTFMKVGQNLLVRRQLEMEKQLKEKMIHSVMPPKVADMLLNEGGTSGLDATGMPPESHYMRPRASNDVKSLFRPFHMHSMENVSILFADIVGFTRMSSTKTAEQLVEILNDLFERFDDLCTLSGCEKISTLGDCYYCVSGCPEPRADHAICCVEMGLGMIDAMRCFDAQRHEGVKMRVGVHTGTVLCGIVGTRRVKFDVWSNDVSLANKMESSGKPEQVHISQETSSFLGDAYYLEEGEEVFGHRTYFVVGRRRDFSRTNSLSPSMPPNPGGSSLLLPGVHASLSQSATNVSVVQPHVPPASPVGQLSSSLNPSPVLSMRPRLTSLSMKLRKKSQSRERERERDVERGIIHPAAAGVPPVIVVRERPKIIITTKSLPGSLDSDEQPPSSPPIKAPPAAPQPPAESRPKIRLKIWNRVPRFLKRRNSSYDSEENREKVHGQDVEKGIDREREKEQEREDQALNPEETLSFMDNQIQNGNGCGYQQLPVLVEATTNRVQTLDIPPARPVLHHAATSTALASSVLRSPEPSTGGCCSPGQYSMYDDIIDVRSYISQSRSDISPFGRSGSYRSQCGRQSMGGGAPLVGAPVAGTSGAAPGVSTQVTPSSCVEQSPLPRPRASTLATGRPGASLEPGTSQNPSPSCLPAPGGPGVPGLSGFGGMFPPTHSRQSSICPSATSRKDSGIKSNSRRSSIQQQIYVLNQSAISQHRVSGYFTSSTSSISNLGEVQGMAPGAMAGVAAPPPPLLMNACSSQIADPLAACLQQLRKQSDLQLIRCVRDNARSQRSYLVKPPLRGFSLYFKSRQLERDFRSKAHRFGAENETEGPPTLATPRYNTYIDIFVGIAVYLCISISLFLMTQNTVSPSFRLWVTLFTCFTGIQVFALFLFTRQMCRRQSGSSAGSANHRLRSKSTTSDDVEARRQTEDRGETPAPQQFESCADRIFEAISSWYPWHICLAILMAMPVLLIIANFLLLDLEQLEAFEYHYGFLIFVCIVHFCNFTQLNCWVRNVLAFLAALCFIAIAMSQLMVYDSRVEDQQDDRGANFIEEIKWFQDYHVEIYLDLLLILVLVWFLNREFEIGYRLTFYGNAVANQDKVRVQNMKNQADMLLHNIIPKHVAEHLKNTAKYSENHHNIAIIFASIVNFNEMYDESYLGGKEFLRVLNELIGDFDELLSRPEFRAVEKIKTIGSTFMAASGLDPSHRGTGDEHIHTLMEFSIAMQEVVDAFNKDLLEFNLILRIGMNIGDVTAGVIGTSKLYYDIWGDAVNVASRMDSTGLPNRIQVGKDCLPFLQSRYEFEPRGSVYVKGKDHMEVFLYTGRMEKLTEEAEVPEKPEKRESVVWMEDLTNPEDNEDDEEEEEEDDLHSSETTTLFKSQESLHANGVSNSHPSPTLNQNQNSNPNPAMPVET

Target ORF information:

RefSeq Version	XM_001966553.2
Organism	Drosophila ananassae
Definition	Drosophila ananassae uncharacterized protein, transcript variant A (Dana\GF22254), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001966553.2

ORF Insert Sequence:

ATGCCGCCGG GCGTTCTGGC CAACGATAGC CGGGCCAACT CCACCGATGA CATCCAGATC 
GCCCTCGCCC CCCACATCCA GACCTACCTG AGCCAAACGG GACGCCGGCA CTCCTGCTGC 
AGTGTCATGC TGCCGGTGGC GTTCGAGCGG GCGGCGGCCA AGTCCTGGCT GGATCCCAAG 
TTCGACTCGC CCGTGCTCGA GGAGCAGTAC CAGACCAGTG TCTATCCCCA TGTCCGAATG 
CGTTACAGAT TCACTTTGTC GTACATTTTG CTCTGCTCCC TGATGTGGTG TTTGTACTTC 
GTGGTGGACG GCGGATCGGA GGACTTCTGG CGGCCCATCT CCAGCTCCTT CTCGATGCTC 
TCCCTGGTGA CGATCATGGC GCTGTGCTTC ACCCACTGGG ACCTGTACAG GGAGCACCGG 
ACAGTGACCT CGGCGGTGAC GGCGCTGCTC CTCTGCGGCG CCTCCCTGGC CTTCCTGACG 
TACACGGGCC GGGCGTTCAG TCCCCTGGGC CACTTTGCCA TATGCCTGGA GATCGTGCTG 
CTGATCTACA CGGCCCTGCC GATGCCCCTG TGGCTGGGAG CCGGCATAGC CATCAGTTAC 
TCCATAGCCT TCGAGATGGT CTCCCACATG GTGATCGGAT GCAGTGCCAT CCATGGCAGT 
GGCTCGACCG AAGGTGTCAC CGGATCAGTG CCCGCCAACG GGGATCCCAG CAACAAGATC 
CTAATACTCC GAATCATGGC CCATTTGAGT GTCCACCTGG TGGGGGTCCA TATCCTGGTG 
ATGAATCTGG TCCGGATGCG AGGCACCTTC ATGAAGGTCG GCCAGAATCT GCTGGTGCGC 
CGCCAACTGG AGATGGAGAA GCAGCTGAAG GAGAAGATGA TACACTCGGT GATGCCCCCG 
AAAGTGGCGG ACATGCTGCT GAACGAGGGC GGCACCTCCG GCCTGGATGC CACCGGAATG 
CCGCCCGAAT CCCACTACAT GCGTCCCCGG GCCTCCAACG ATGTGAAGTC CCTGTTCCGG 
CCCTTCCACA TGCACAGCAT GGAGAATGTC AGCATCCTGT TTGCGGACAT CGTCGGCTTC 
ACTCGCATGT CCTCCACGAA GACCGCCGAG CAGCTGGTCG AGATCCTCAA CGATCTCTTC 
GAGCGCTTCG ACGACCTCTG CACGCTGAGT GGCTGCGAGA AGATCTCCAC CCTGGGCGAC 
TGCTACTACT GTGTCTCCGG ATGCCCGGAG CCCCGGGCGG ATCATGCCAT CTGCTGTGTG 
GAGATGGGTC TGGGCATGAT CGATGCGATG CGCTGTTTCG ACGCCCAGCG GCACGAGGGC 
GTCAAGATGC GGGTTGGCGT CCATACGGGC ACAGTCCTGT GCGGCATTGT GGGCACCCGG 
CGGGTCAAGT TCGATGTCTG GAGCAATGAC GTTAGTTTGG CCAACAAAAT GGAATCCTCT 
GGCAAACCGG AACAAGTCCA CATCTCCCAG GAGACGTCCA GCTTTCTGGG GGATGCCTAC 
TACCTGGAAG AGGGCGAGGA AGTTTTCGGA CACCGTACTT ACTTCGTGGT GGGTCGTCGT 
CGGGACTTCT CCCGCACCAA TAGCCTGAGT CCCAGCATGC CGCCGAACCC GGGCGGCAGC 
TCCCTGCTCC TGCCCGGCGT CCATGCCAGC TTGTCGCAGA GCGCCACCAA TGTCTCGGTG 
GTGCAGCCGC ACGTCCCGCC CGCCTCGCCG GTGGGCCAGC TCTCCAGCTC CCTGAATCCA 
TCCCCAGTGC TCTCCATGCG CCCCCGGCTC ACCTCCCTGA GCATGAAGCT GCGCAAGAAG 
TCGCAGAGTC GGGAGCGGGA GAGGGAGAGG GACGTGGAGC GGGGCATCAT CCATCCGGCG 
GCGGCGGGAG TTCCGCCAGT GATTGTGGTG CGGGAGCGAC CCAAGATCAT CATCACCACC 
AAGTCCCTGC CCGGCAGCCT GGACTCGGAC GAGCAGCCGC CCAGTAGTCC GCCCATCAAG 
GCGCCGCCCG CCGCCCCCCA GCCGCCGGCC GAGTCGCGTC CAAAGATCCG ATTGAAGATC 
TGGAACCGAG TGCCGAGATT CCTGAAGCGG CGCAACAGCT CCTACGACTC GGAGGAGAAC 
AGGGAGAAGG TCCACGGCCA GGATGTGGAG AAGGGCATCG ACCGGGAGAG GGAGAAGGAG 
CAGGAGCGCG AGGATCAGGC CCTGAATCCG GAGGAGACGC TGTCCTTCAT GGATAACCAG 
ATCCAGAACG GCAACGGATG CGGCTACCAG CAGCTGCCCG TCCTGGTGGA GGCCACCACG 
AATCGCGTCC AGACTCTGGA CATCCCGCCG GCCCGGCCAG TCCTCCATCA CGCCGCCACC 
TCGACGGCCC TTGCCAGCAG TGTCCTGCGA TCCCCGGAGC CTAGCACCGG CGGATGCTGC 
TCGCCGGGCC AGTACTCCAT GTACGACGAC ATCATCGACG TGAGGTCCTA CATCAGCCAG 
TCGAGGAGCG ACATCTCGCC GTTCGGCCGG TCTGGGAGCT ACCGATCCCA GTGCGGCAGG 
CAGTCCATGG GCGGAGGAGC ACCTCTAGTC GGAGCACCGG TGGCGGGAAC GTCGGGCGCA 
GCCCCGGGAG TATCCACGCA GGTGACACCA TCGTCCTGTG TGGAGCAGTC CCCGTTGCCA 
CGTCCCCGGG CCTCGACACT GGCCACTGGA CGGCCCGGAG CCAGCCTGGA GCCCGGAACA 
TCACAGAATC CCAGTCCCAG TTGCCTGCCA GCGCCGGGCG GACCCGGAGT ACCCGGTCTG 
TCCGGCTTCG GGGGCATGTT TCCGCCCACC CACTCGCGGC AGAGCAGCAT CTGCCCGTCG 
GCCACCTCGC GGAAGGACTC GGGCATCAAG AGCAACTCCC GCCGGTCCTC CATCCAGCAG 
CAGATCTACG TCCTGAACCA GTCGGCCATT AGCCAGCACA GGGTGTCCGG ATACTTCACC 
AGTTCCACCT CCAGCATCTC GAATTTGGGC GAGGTCCAGG GCATGGCTCC GGGGGCCATG 
GCGGGAGTGG CCGCCCCACC GCCACCCCTC CTGATGAACG CCTGCTCCTC GCAGATAGCC 
GATCCGCTGG CGGCCTGCCT CCAGCAGCTG AGGAAGCAAT CGGACCTGCA GCTCATCCGA 
TGCGTCCGGG ACAATGCCAG GTCGCAGAGG AGCTACCTGG TGAAGCCGCC GCTCCGGGGC 
TTCAGCCTGT ACTTCAAGTC CCGGCAGCTG GAGCGGGACT TCCGGTCGAA GGCCCACCGC 
TTCGGGGCCG AGAACGAAAC GGAAGGACCT CCCACCCTGG CCACGCCCCG CTACAACACC 
TACATAGACA TCTTCGTGGG CATCGCCGTC TACCTGTGCA TCTCGATATC CCTCTTCCTG 
ATGACCCAGA ACACGGTGAG CCCCAGCTTC CGGCTGTGGG TGACACTGTT CACCTGCTTC 
ACGGGCATCC AGGTGTTCGC TCTGTTCCTC TTCACCCGCC AGATGTGCCG GCGGCAGAGT 
GGGTCGAGTG CCGGCAGTGC CAACCACCGA TTGCGCTCCA AGTCCACCAC CAGCGATGAT 
GTGGAGGCCA GGCGCCAGAC CGAGGATAGG GGCGAGACAC CAGCTCCCCA GCAGTTCGAG 
TCCTGTGCCG ATCGCATCTT TGAGGCCATC TCCAGCTGGT ATCCGTGGCA CATCTGCCTG 
GCCATCCTGA TGGCGATGCC CGTCCTGCTG ATTATCGCCA ACTTCCTGCT GCTGGACCTG 
GAGCAGCTGG AGGCCTTCGA ATACCACTAC GGCTTCCTGA TCTTCGTCTG CATCGTCCAC 
TTCTGCAACT TCACCCAGCT GAACTGCTGG GTGCGGAATG TCCTGGCCTT CCTGGCGGCC 
CTCTGCTTCA TCGCCATCGC CATGTCCCAG CTGATGGTGT ACGACAGTCG GGTGGAGGAC 
CAGCAGGACG ACAGGGGCGC CAACTTCATT GAGGAGATCA AGTGGTTCCA GGACTACCAC 
GTCGAGATCT ACTTGGACCT GTTGCTCATC CTGGTGCTGG TGTGGTTCCT CAATCGCGAA 
TTCGAGATTG GGTATCGGCT GACCTTCTAC GGCAATGCCG TCGCCAACCA GGACAAGGTC 
CGCGTCCAAA ACATGAAGAA CCAGGCGGAC ATGCTGCTCC ACAACATCAT ACCCAAGCAC 
GTGGCGGAGC ACCTGAAGAA CACGGCCAAG TACTCCGAGA ACCACCACAA CATAGCCATT 
ATATTCGCAT CGATAGTCAA TTTCAACGAG ATGTACGACG AGAGCTATCT GGGGGGGAAG 
GAGTTCCTCC GGGTCCTCAA CGAGCTGATC GGGGACTTTG ATGAGCTGCT CTCCCGGCCG 
GAGTTCCGGG CGGTGGAGAA GATCAAGACG ATCGGCTCCA CCTTCATGGC GGCCAGTGGC 
CTGGATCCTT CCCATCGGGG CACTGGCGAC GAGCACATCC ACACCCTGAT GGAGTTCTCC 
ATCGCCATGC AGGAGGTGGT GGACGCCTTC AACAAGGACC TCCTCGAGTT CAACCTAATC 
CTGAGGATCG GCATGAATAT CGGAGATGTT ACAGCCGGAG TGATCGGCAC CAGTAAGCTC 
TACTACGACA TCTGGGGGGA TGCGGTGAAT GTGGCCTCCC GGATGGACTC CACGGGCCTG 
CCCAATCGCA TCCAGGTGGG CAAGGACTGC CTGCCCTTCC TGCAGAGCCG CTACGAGTTC 
GAGCCCAGGG GCAGTGTGTA TGTGAAGGGC AAGGATCACA TGGAGGTGTT CCTCTACACA 
GGACGAATGG AGAAGCTGAC CGAAGAGGCG GAAGTTCCAG AGAAGCCAGA GAAGCGCGAA 
TCAGTCGTAT GGATGGAGGA TCTAACTAAC CCGGAAGACA ACGAGGACGA CGAGGAGGAG 
GAGGAGGAGG ATGATCTGCA CTCCAGTGAG ACCACCACCC TCTTCAAATC CCAGGAGTCA 
CTTCACGCCA ATGGTGTCTC CAATTCCCAT CCAAGTCCAA CTCTGAATCA GAATCAGAAT 
TCGAATCCGA ATCCAGCCAT GCCAGTGGAG ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dana\GF22254 ( XM_014904369.1 ) cDNA ORF clone, Drosophila ananassae -> XP_014759855.1 Drosophila ananassae uncharacterized protein, transcript variant B (Dana\GF22254), mRNA.

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CloneID	ODh15777
Clone ID Related Accession (Same CDS sequence)	XM_014904369.1
Accession Version	XM_014904369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450108800000
Organism	Drosophila ananassae
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001939307). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGAGT CGCGTCGTCA GTCGGTGAGC TTAACGGCTA TGCCGCCGGG CGTTCTGGCC 
AACGATAGCC GGGCCAACTC CACCGATGAC ATCCAGATCG CCCTCGCCCC CCACATCCAG 
ACCTACCTGA GCCAAACGGG ACGCCGGCAC TCCTGCTGCA GTGTCATGCT GCCGGTGGCG 
TTCGAGCGGG CGGCGGCCAA GTCCTGGCTG GATCCCAAGT TCGACTCGCC CGTGCTCGAG 
GAGCAGTACC AGACCAGTGT CTATCCCCAT GTCCGAATGC GTTACAGATT CACTTTGTCG 
TACATTTTGC TCTGCTCCCT GATGTGGTGT TTGTACTTCG TGGTGGACGG CGGATCGGAG 
GACTTCTGGC GGCCCATCTC CAGCTCCTTC TCGATGCTCT CCCTGGTGAC GATCATGGCG 
CTGTGCTTCA CCCACTGGGA CCTGTACAGG GAGCACCGGA CAGTGACCTC GGCGGTGACG 
GCGCTGCTCC TCTGCGGCGC CTCCCTGGCC TTCCTGACGT ACACGGGCCG GGCGTTCAGT 
CCCCTGGGCC ACTTTGCCAT ATGCCTGGAG ATCGTGCTGC TGATCTACAC GGCCCTGCCG 
ATGCCCCTGT GGCTGGGAGC CGGCATAGCC ATCAGTTACT CCATAGCCTT CGAGATGGTC 
TCCCACATGG TGATCGGATG CAGTGCCATC CATGGCAGTG GCTCGACCGA AGGTGTCACC 
GGATCAGTGC CCGCCAACGG GGATCCCAGC AACAAGATCC TAATACTCCG AATCATGGCC 
CATTTGAGTG TCCACCTGGT GGGGGTCCAT ATCCTGGTGA TGAATCTGGT CCGGATGCGA 
GGCACCTTCA TGAAGGTCGG CCAGAATCTG CTGGTGCGCC GCCAACTGGA GATGGAGAAG 
CAGCTGAAGG AGAAGATGAT ACACTCGGTG ATGCCCCCGA AAGTGGCGGA CATGCTGCTG 
AACGAGGGCG GCACCTCCGG CCTGGATGCC ACCGGAATGC CGCCCGAATC CCACTACATG 
CGTCCCCGGG CCTCCAACGA TGTGAAGTCC CTGTTCCGGC CCTTCCACAT GCACAGCATG 
GAGAATGTCA GCATCCTGTT TGCGGACATC GTCGGCTTCA CTCGCATGTC CTCCACGAAG 
ACCGCCGAGC AGCTGGTCGA GATCCTCAAC GATCTCTTCG AGCGCTTCGA CGACCTCTGC 
ACGCTGAGTG GCTGCGAGAA GATCTCCACC CTGGGCGACT GCTACTACTG TGTCTCCGGA 
TGCCCGGAGC CCCGGGCGGA TCATGCCATC TGCTGTGTGG AGATGGGTCT GGGCATGATC 
GATGCGATGC GCTGTTTCGA CGCCCAGCGG CACGAGGGCG TCAAGATGCG GGTTGGCGTC 
CATACGGGCA CAGTCCTGTG CGGCATTGTG GGCACCCGGC GGGTCAAGTT CGATGTCTGG 
AGCAATGACG TTAGTTTGGC CAACAAAATG GAATCCTCTG GCAAACCGGA ACAAGTCCAC 
ATCTCCCAGG AGACGTCCAG CTTTCTGGGG GATGCCTACT ACCTGGAAGA GGGCGAGGAA 
GTTTTCGGAC ACCGTACTTA CTTCGTGGTG GGTCGTCGTC GGGACTTCTC CCGCACCAAT 
AGCCTGAGTC CCAGCATGCC GCCGAACCCG GGCGGCAGCT CCCTGCTCCT GCCCGGCGTC 
CATGCCAGCT TGTCGCAGAG CGCCACCAAT GTCTCGGTGG TGCAGCCGCA CGTCCCGCCC 
GCCTCGCCGG TGGGCCAGCT CTCCAGCTCC CTGAATCCAT CCCCAGTGCT CTCCATGCGC 
CCCCGGCTCA CCTCCCTGAG CATGAAGCTG CGCAAGAAGT CGCAGAGTCG GGAGCGGGAG 
AGGGAGAGGG ACGTGGAGCG GGGCATCATC CATCCGGCGG CGGCGGGAGT TCCGCCAGTG 
ATTGTGGTGC GGGAGCGACC CAAGATCATC ATCACCACCA AGTCCCTGCC CGGCAGCCTG 
GACTCGGACG AGCAGCCGCC CAGTAGTCCG CCCATCAAGG CGCCGCCCGC CGCCCCCCAG 
CCGCCGGCCG AGTCGCGTCC AAAGATCCGA TTGAAGATCT GGAACCGAGT GCCGAGATTC 
CTGAAGCGGC GCAACAGCTC CTACGACTCG GAGGAGAACA GGGAGAAGGT CCACGGCCAG 
GATGTGGAGA AGGGCATCGA CCGGGAGAGG GAGAAGGAGC AGGAGCGCGA GGATCAGGCC 
CTGAATCCGG AGGAGACGCT GTCCTTCATG GATAACCAGA TCCAGAACGG CAACGGATGC 
GGCTACCAGC AGCTGCCCGT CCTGGTGGAG GCCACCACGA ATCGCGTCCA GACTCTGGAC 
ATCCCGCCGG CCCGGCCAGT CCTCCATCAC GCCGCCACCT CGACGGCCCT TGCCAGCAGT 
GTCCTGCGAT CCCCGGAGCC TAGCACCGGC GGATGCTGCT CGCCGGGCCA GTACTCCATG 
TACGACGACA TCATCGACGT GAGGTCCTAC ATCAGCCAGT CGAGGAGCGA CATCTCGCCG 
TTCGGCCGGT CTGGGAGCTA CCGATCCCAG TGCGGCAGGC AGTCCATGGG CGGAGGAGCA 
CCTCTAGTCG GAGCACCGGT GGCGGGAACG TCGGGCGCAG CCCCGGGAGT ATCCACGCAG 
GTGACACCAT CGTCCTGTGT GGAGCAGTCC CCGTTGCCAC GTCCCCGGGC CTCGACACTG 
GCCACTGGAC GGCCCGGAGC CAGCCTGGAG CCCGGAACAT CACAGAATCC CAGTCCCAGT 
TGCCTGCCAG CGCCGGGCGG ACCCGGAGTA CCCGGTCTGT CCGGCTTCGG GGGCATGTTT 
CCGCCCACCC ACTCGCGGCA GAGCAGCATC TGCCCGTCGG CCACCTCGCG GAAGGACTCG 
GGCATCAAGA GCAACTCCCG CCGGTCCTCC ATCCAGCAGC AGATCTACGT CCTGAACCAG 
TCGGCCATTA GCCAGCACAG GGTGTCCGGA TACTTCACCA GTTCCACCTC CAGCATCTCG 
AATTTGGGCG AGGTCCAGGG CATGGCTCCG GGGGCCATGG CGGGAGTGGC CGCCCCACCG 
CCACCCCTCC TGATGAACGC CTGCTCCTCG CAGATAGCCG ATCCGCTGGC GGCCTGCCTC 
CAGCAGCTGA GGAAGCAATC GGACCTGCAG CTCATCCGAT GCGTCCGGGA CAATGCCAGG 
TCGCAGAGGA GCTACCTGGT GAAGCCGCCG CTCCGGGGCT TCAGCCTGTA CTTCAAGTCC 
CGGCAGCTGG AGCGGGACTT CCGGTCGAAG GCCCACCGCT TCGGGGCCGA GAACGAAACG 
GAAGGACCTC CCACCCTGGC CACGCCCCGC TACAACACCT ACATAGACAT CTTCGTGGGC 
ATCGCCGTCT ACCTGTGCAT CTCGATATCC CTCTTCCTGA TGACCCAGAA CACGGTGAGC 
CCCAGCTTCC GGCTGTGGGT GACACTGTTC ACCTGCTTCA CGGGCATCCA GGTGTTCGCT 
CTGTTCCTCT TCACCCGCCA GATGTGCCGG CGGCAGAGTG GGTCGAGTGC CGGCAGTGCC 
AACCACCGAT TGCGCTCCAA GTCCACCACC AGCGATGATG TGGAGGCCAG GCGCCAGACC 
GAGGATAGGG GCGAGACACC AGCTCCCCAG CAGTTCGAGT CCTGTGCCGA TCGCATCTTT 
GAGGCCATCT CCAGCTGGTA TCCGTGGCAC ATCTGCCTGG CCATCCTGAT GGCGATGCCC 
GTCCTGCTGA TTATCGCCAA CTTCCTGCTG CTGGACCTGG AGCAGCTGGA GGCCTTCGAA 
TACCACTACG GCTTCCTGAT CTTCGTCTGC ATCGTCCACT TCTGCAACTT CACCCAGCTG 
AACTGCTGGG TGCGGAATGT CCTGGCCTTC CTGGCGGCCC TCTGCTTCAT CGCCATCGCC 
ATGTCCCAGC TGATGGTGTA CGACAGTCGG GTGGAGGACC AGCAGGACGA CAGGGGCGCC 
AACTTCATTG AGGAGATCAA GTGGTTCCAG GACTACCACG TCGAGATCTA CTTGGACCTG 
TTGCTCATCC TGGTGCTGGT GTGGTTCCTC AATCGCGAAT TCGAGATTGG GTATCGGCTG 
ACCTTCTACG GCAATGCCGT CGCCAACCAG GACAAGGTCC GCGTCCAAAA CATGAAGAAC 
CAGGCGGACA TGCTGCTCCA CAACATCATA CCCAAGCACG TGGCGGAGCA CCTGAAGAAC 
ACGGCCAAGT ACTCCGAGAA CCACCACAAC ATAGCCATTA TATTCGCATC GATAGTCAAT 
TTCAACGAGA TGTACGACGA GAGCTATCTG GGGGGGAAGG AGTTCCTCCG GGTCCTCAAC 
GAGCTGATCG GGGACTTTGA TGAGCTGCTC TCCCGGCCGG AGTTCCGGGC GGTGGAGAAG 
ATCAAGACGA TCGGCTCCAC CTTCATGGCG GCCAGTGGCC TGGATCCTTC CCATCGGGGC 
ACTGGCGACG AGCACATCCA CACCCTGATG GAGTTCTCCA TCGCCATGCA GGAGGTGGTG 
GACGCCTTCA ACAAGGACCT CCTCGAGTTC AACCTAATCC TGAGGATCGG CATGAATATC 
GGAGATGTTA CAGCCGGAGT GATCGGCACC AGTAAGCTCT ACTACGACAT CTGGGGGGAT 
GCGGTGAATG TGGCCTCCCG GATGGACTCC ACGGGCCTGC CCAATCGCAT CCAGGTGGGC 
AAGGACTGCC TGCCCTTCCT GCAGAGCCGC TACGAGTTCG AGCCCAGGGG CAGTGTGTAT 
GTGAAGGGCA AGGATCACAT GGAGGTGTTC CTCTACACAG GACGAATGGA GAAGCTGACC 
GAAGAGGCGG AAGTTCCAGA GAAGCCAGAG AAGCGCGAAT CAGTCGTATG GATGGAGGAT 
CTAACTAACC CGGAAGACAA CGAGGACGAC GAGGAGGAGG AGGAGGAGGA TGATCTGCAC 
TCCAGTGAGA CCACCACCCT CTTCAAATCC CAGGAGTCAC TTCACGCCAA TGGTGTCTCC 
AATTCCCATC CAAGTCCAAC TCTGAATCAG AATCAGAATT CGAATCCGAA TCCAGCCATG 
CCAGTGGAGA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014759855.1
CDS	99..5153
Translation	MSESRRQSVSLTAMPPGVLANDSRANSTDDIQIALAPHIQTYLSQTGRRHSCCSVMLPVAFERAAAKSWLDPKFDSPVLEEQYQTSVYPHVRMRYRFTLSYILLCSLMWCLYFVVDGGSEDFWRPISSSFSMLSLVTIMALCFTHWDLYREHRTVTSAVTALLLCGASLAFLTYTGRAFSPLGHFAICLEIVLLIYTALPMPLWLGAGIAISYSIAFEMVSHMVIGCSAIHGSGSTEGVTGSVPANGDPSNKILILRIMAHLSVHLVGVHILVMNLVRMRGTFMKVGQNLLVRRQLEMEKQLKEKMIHSVMPPKVADMLLNEGGTSGLDATGMPPESHYMRPRASNDVKSLFRPFHMHSMENVSILFADIVGFTRMSSTKTAEQLVEILNDLFERFDDLCTLSGCEKISTLGDCYYCVSGCPEPRADHAICCVEMGLGMIDAMRCFDAQRHEGVKMRVGVHTGTVLCGIVGTRRVKFDVWSNDVSLANKMESSGKPEQVHISQETSSFLGDAYYLEEGEEVFGHRTYFVVGRRRDFSRTNSLSPSMPPNPGGSSLLLPGVHASLSQSATNVSVVQPHVPPASPVGQLSSSLNPSPVLSMRPRLTSLSMKLRKKSQSRERERERDVERGIIHPAAAGVPPVIVVRERPKIIITTKSLPGSLDSDEQPPSSPPIKAPPAAPQPPAESRPKIRLKIWNRVPRFLKRRNSSYDSEENREKVHGQDVEKGIDREREKEQEREDQALNPEETLSFMDNQIQNGNGCGYQQLPVLVEATTNRVQTLDIPPARPVLHHAATSTALASSVLRSPEPSTGGCCSPGQYSMYDDIIDVRSYISQSRSDISPFGRSGSYRSQCGRQSMGGGAPLVGAPVAGTSGAAPGVSTQVTPSSCVEQSPLPRPRASTLATGRPGASLEPGTSQNPSPSCLPAPGGPGVPGLSGFGGMFPPTHSRQSSICPSATSRKDSGIKSNSRRSSIQQQIYVLNQSAISQHRVSGYFTSSTSSISNLGEVQGMAPGAMAGVAAPPPPLLMNACSSQIADPLAACLQQLRKQSDLQLIRCVRDNARSQRSYLVKPPLRGFSLYFKSRQLERDFRSKAHRFGAENETEGPPTLATPRYNTYIDIFVGIAVYLCISISLFLMTQNTVSPSFRLWVTLFTCFTGIQVFALFLFTRQMCRRQSGSSAGSANHRLRSKSTTSDDVEARRQTEDRGETPAPQQFESCADRIFEAISSWYPWHICLAILMAMPVLLIIANFLLLDLEQLEAFEYHYGFLIFVCIVHFCNFTQLNCWVRNVLAFLAALCFIAIAMSQLMVYDSRVEDQQDDRGANFIEEIKWFQDYHVEIYLDLLLILVLVWFLNREFEIGYRLTFYGNAVANQDKVRVQNMKNQADMLLHNIIPKHVAEHLKNTAKYSENHHNIAIIFASIVNFNEMYDESYLGGKEFLRVLNELIGDFDELLSRPEFRAVEKIKTIGSTFMAASGLDPSHRGTGDEHIHTLMEFSIAMQEVVDAFNKDLLEFNLILRIGMNIGDVTAGVIGTSKLYYDIWGDAVNVASRMDSTGLPNRIQVGKDCLPFLQSRYEFEPRGSVYVKGKDHMEVFLYTGRMEKLTEEAEVPEKPEKRESVVWMEDLTNPEDNEDDEEEEEEDDLHSSETTTLFKSQESLHANGVSNSHPSPTLNQNQNSNPNPAMPVET

Target ORF information:

RefSeq Version	XM_014904369.1
Organism	Drosophila ananassae
Definition	Drosophila ananassae uncharacterized protein, transcript variant B (Dana\GF22254), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014904369.1

ORF Insert Sequence:

ATGTCCGAGT CGCGTCGTCA GTCGGTGAGC TTAACGGCTA TGCCGCCGGG CGTTCTGGCC 
AACGATAGCC GGGCCAACTC CACCGATGAC ATCCAGATCG CCCTCGCCCC CCACATCCAG 
ACCTACCTGA GCCAAACGGG ACGCCGGCAC TCCTGCTGCA GTGTCATGCT GCCGGTGGCG 
TTCGAGCGGG CGGCGGCCAA GTCCTGGCTG GATCCCAAGT TCGACTCGCC CGTGCTCGAG 
GAGCAGTACC AGACCAGTGT CTATCCCCAT GTCCGAATGC GTTACAGATT CACTTTGTCG 
TACATTTTGC TCTGCTCCCT GATGTGGTGT TTGTACTTCG TGGTGGACGG CGGATCGGAG 
GACTTCTGGC GGCCCATCTC CAGCTCCTTC TCGATGCTCT CCCTGGTGAC GATCATGGCG 
CTGTGCTTCA CCCACTGGGA CCTGTACAGG GAGCACCGGA CAGTGACCTC GGCGGTGACG 
GCGCTGCTCC TCTGCGGCGC CTCCCTGGCC TTCCTGACGT ACACGGGCCG GGCGTTCAGT 
CCCCTGGGCC ACTTTGCCAT ATGCCTGGAG ATCGTGCTGC TGATCTACAC GGCCCTGCCG 
ATGCCCCTGT GGCTGGGAGC CGGCATAGCC ATCAGTTACT CCATAGCCTT CGAGATGGTC 
TCCCACATGG TGATCGGATG CAGTGCCATC CATGGCAGTG GCTCGACCGA AGGTGTCACC 
GGATCAGTGC CCGCCAACGG GGATCCCAGC AACAAGATCC TAATACTCCG AATCATGGCC 
CATTTGAGTG TCCACCTGGT GGGGGTCCAT ATCCTGGTGA TGAATCTGGT CCGGATGCGA 
GGCACCTTCA TGAAGGTCGG CCAGAATCTG CTGGTGCGCC GCCAACTGGA GATGGAGAAG 
CAGCTGAAGG AGAAGATGAT ACACTCGGTG ATGCCCCCGA AAGTGGCGGA CATGCTGCTG 
AACGAGGGCG GCACCTCCGG CCTGGATGCC ACCGGAATGC CGCCCGAATC CCACTACATG 
CGTCCCCGGG CCTCCAACGA TGTGAAGTCC CTGTTCCGGC CCTTCCACAT GCACAGCATG 
GAGAATGTCA GCATCCTGTT TGCGGACATC GTCGGCTTCA CTCGCATGTC CTCCACGAAG 
ACCGCCGAGC AGCTGGTCGA GATCCTCAAC GATCTCTTCG AGCGCTTCGA CGACCTCTGC 
ACGCTGAGTG GCTGCGAGAA GATCTCCACC CTGGGCGACT GCTACTACTG TGTCTCCGGA 
TGCCCGGAGC CCCGGGCGGA TCATGCCATC TGCTGTGTGG AGATGGGTCT GGGCATGATC 
GATGCGATGC GCTGTTTCGA CGCCCAGCGG CACGAGGGCG TCAAGATGCG GGTTGGCGTC 
CATACGGGCA CAGTCCTGTG CGGCATTGTG GGCACCCGGC GGGTCAAGTT CGATGTCTGG 
AGCAATGACG TTAGTTTGGC CAACAAAATG GAATCCTCTG GCAAACCGGA ACAAGTCCAC 
ATCTCCCAGG AGACGTCCAG CTTTCTGGGG GATGCCTACT ACCTGGAAGA GGGCGAGGAA 
GTTTTCGGAC ACCGTACTTA CTTCGTGGTG GGTCGTCGTC GGGACTTCTC CCGCACCAAT 
AGCCTGAGTC CCAGCATGCC GCCGAACCCG GGCGGCAGCT CCCTGCTCCT GCCCGGCGTC 
CATGCCAGCT TGTCGCAGAG CGCCACCAAT GTCTCGGTGG TGCAGCCGCA CGTCCCGCCC 
GCCTCGCCGG TGGGCCAGCT CTCCAGCTCC CTGAATCCAT CCCCAGTGCT CTCCATGCGC 
CCCCGGCTCA CCTCCCTGAG CATGAAGCTG CGCAAGAAGT CGCAGAGTCG GGAGCGGGAG 
AGGGAGAGGG ACGTGGAGCG GGGCATCATC CATCCGGCGG CGGCGGGAGT TCCGCCAGTG 
ATTGTGGTGC GGGAGCGACC CAAGATCATC ATCACCACCA AGTCCCTGCC CGGCAGCCTG 
GACTCGGACG AGCAGCCGCC CAGTAGTCCG CCCATCAAGG CGCCGCCCGC CGCCCCCCAG 
CCGCCGGCCG AGTCGCGTCC AAAGATCCGA TTGAAGATCT GGAACCGAGT GCCGAGATTC 
CTGAAGCGGC GCAACAGCTC CTACGACTCG GAGGAGAACA GGGAGAAGGT CCACGGCCAG 
GATGTGGAGA AGGGCATCGA CCGGGAGAGG GAGAAGGAGC AGGAGCGCGA GGATCAGGCC 
CTGAATCCGG AGGAGACGCT GTCCTTCATG GATAACCAGA TCCAGAACGG CAACGGATGC 
GGCTACCAGC AGCTGCCCGT CCTGGTGGAG GCCACCACGA ATCGCGTCCA GACTCTGGAC 
ATCCCGCCGG CCCGGCCAGT CCTCCATCAC GCCGCCACCT CGACGGCCCT TGCCAGCAGT 
GTCCTGCGAT CCCCGGAGCC TAGCACCGGC GGATGCTGCT CGCCGGGCCA GTACTCCATG 
TACGACGACA TCATCGACGT GAGGTCCTAC ATCAGCCAGT CGAGGAGCGA CATCTCGCCG 
TTCGGCCGGT CTGGGAGCTA CCGATCCCAG TGCGGCAGGC AGTCCATGGG CGGAGGAGCA 
CCTCTAGTCG GAGCACCGGT GGCGGGAACG TCGGGCGCAG CCCCGGGAGT ATCCACGCAG 
GTGACACCAT CGTCCTGTGT GGAGCAGTCC CCGTTGCCAC GTCCCCGGGC CTCGACACTG 
GCCACTGGAC GGCCCGGAGC CAGCCTGGAG CCCGGAACAT CACAGAATCC CAGTCCCAGT 
TGCCTGCCAG CGCCGGGCGG ACCCGGAGTA CCCGGTCTGT CCGGCTTCGG GGGCATGTTT 
CCGCCCACCC ACTCGCGGCA GAGCAGCATC TGCCCGTCGG CCACCTCGCG GAAGGACTCG 
GGCATCAAGA GCAACTCCCG CCGGTCCTCC ATCCAGCAGC AGATCTACGT CCTGAACCAG 
TCGGCCATTA GCCAGCACAG GGTGTCCGGA TACTTCACCA GTTCCACCTC CAGCATCTCG 
AATTTGGGCG AGGTCCAGGG CATGGCTCCG GGGGCCATGG CGGGAGTGGC CGCCCCACCG 
CCACCCCTCC TGATGAACGC CTGCTCCTCG CAGATAGCCG ATCCGCTGGC GGCCTGCCTC 
CAGCAGCTGA GGAAGCAATC GGACCTGCAG CTCATCCGAT GCGTCCGGGA CAATGCCAGG 
TCGCAGAGGA GCTACCTGGT GAAGCCGCCG CTCCGGGGCT TCAGCCTGTA CTTCAAGTCC 
CGGCAGCTGG AGCGGGACTT CCGGTCGAAG GCCCACCGCT TCGGGGCCGA GAACGAAACG 
GAAGGACCTC CCACCCTGGC CACGCCCCGC TACAACACCT ACATAGACAT CTTCGTGGGC 
ATCGCCGTCT ACCTGTGCAT CTCGATATCC CTCTTCCTGA TGACCCAGAA CACGGTGAGC 
CCCAGCTTCC GGCTGTGGGT GACACTGTTC ACCTGCTTCA CGGGCATCCA GGTGTTCGCT 
CTGTTCCTCT TCACCCGCCA GATGTGCCGG CGGCAGAGTG GGTCGAGTGC CGGCAGTGCC 
AACCACCGAT TGCGCTCCAA GTCCACCACC AGCGATGATG TGGAGGCCAG GCGCCAGACC 
GAGGATAGGG GCGAGACACC AGCTCCCCAG CAGTTCGAGT CCTGTGCCGA TCGCATCTTT 
GAGGCCATCT CCAGCTGGTA TCCGTGGCAC ATCTGCCTGG CCATCCTGAT GGCGATGCCC 
GTCCTGCTGA TTATCGCCAA CTTCCTGCTG CTGGACCTGG AGCAGCTGGA GGCCTTCGAA 
TACCACTACG GCTTCCTGAT CTTCGTCTGC ATCGTCCACT TCTGCAACTT CACCCAGCTG 
AACTGCTGGG TGCGGAATGT CCTGGCCTTC CTGGCGGCCC TCTGCTTCAT CGCCATCGCC 
ATGTCCCAGC TGATGGTGTA CGACAGTCGG GTGGAGGACC AGCAGGACGA CAGGGGCGCC 
AACTTCATTG AGGAGATCAA GTGGTTCCAG GACTACCACG TCGAGATCTA CTTGGACCTG 
TTGCTCATCC TGGTGCTGGT GTGGTTCCTC AATCGCGAAT TCGAGATTGG GTATCGGCTG 
ACCTTCTACG GCAATGCCGT CGCCAACCAG GACAAGGTCC GCGTCCAAAA CATGAAGAAC 
CAGGCGGACA TGCTGCTCCA CAACATCATA CCCAAGCACG TGGCGGAGCA CCTGAAGAAC 
ACGGCCAAGT ACTCCGAGAA CCACCACAAC ATAGCCATTA TATTCGCATC GATAGTCAAT 
TTCAACGAGA TGTACGACGA GAGCTATCTG GGGGGGAAGG AGTTCCTCCG GGTCCTCAAC 
GAGCTGATCG GGGACTTTGA TGAGCTGCTC TCCCGGCCGG AGTTCCGGGC GGTGGAGAAG 
ATCAAGACGA TCGGCTCCAC CTTCATGGCG GCCAGTGGCC TGGATCCTTC CCATCGGGGC 
ACTGGCGACG AGCACATCCA CACCCTGATG GAGTTCTCCA TCGCCATGCA GGAGGTGGTG 
GACGCCTTCA ACAAGGACCT CCTCGAGTTC AACCTAATCC TGAGGATCGG CATGAATATC 
GGAGATGTTA CAGCCGGAGT GATCGGCACC AGTAAGCTCT ACTACGACAT CTGGGGGGAT 
GCGGTGAATG TGGCCTCCCG GATGGACTCC ACGGGCCTGC CCAATCGCAT CCAGGTGGGC 
AAGGACTGCC TGCCCTTCCT GCAGAGCCGC TACGAGTTCG AGCCCAGGGG CAGTGTGTAT 
GTGAAGGGCA AGGATCACAT GGAGGTGTTC CTCTACACAG GACGAATGGA GAAGCTGACC 
GAAGAGGCGG AAGTTCCAGA GAAGCCAGAG AAGCGCGAAT CAGTCGTATG GATGGAGGAT 
CTAACTAACC CGGAAGACAA CGAGGACGAC GAGGAGGAGG AGGAGGAGGA TGATCTGCAC 
TCCAGTGAGA CCACCACCCT CTTCAAATCC CAGGAGTCAC TTCACGCCAA TGGTGTCTCC 
AATTCCCATC CAAGTCCAAC TCTGAATCAG AATCAGAATT CGAATCCGAA TCCAGCCATG 
CCAGTGGAGA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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