CNA06050 cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

Gene
Clones
gRNAs

Summary

Gene Symbol	CNA06050
Entrez Gene ID	3253821
Full Name	ARF guanyl-nucleotide exchange factor
Gene Type	protein-coding
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

mRNA and Protein(s)

mRNA	Protein	Name
XM_566959.2	XP_566959.1	ARF guanyl-nucleotide exchange factor, putative
XM_566959.1	XP_566959.1	ARF guanyl-nucleotide exchange factor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
Loftus BJ, Fung E, Roncaglia P, Rowley D, Amedeo P, Bruno D, Vamathevan J, Miranda M, Anderson IJ, Fraser JA, Allen JE, Bosdet IE, Brent MR, Chiu R, Doering TL, Donlin MJ, D'Souza CA, Fox DS, Grinberg V, Fu J, Fukushima M, Haas BJ, Huang JC, Janbon G, Jones SJ, Koo HL, Krzywinski MI, Kwon-Chung JK, Lengeler KB, Maiti R, Marra MA, Marra RE, Mathewson CA, Mitchell TG, Pertea M, Riggs FR, Salzberg SL, Schein JE, Shvartsbeyn A, Shin H, Shumway M, Specht CA, Suh BB, Tenney A, Utterback TR, Wickes BL, Wortman JR, Wye NH, Kronstad JW, Lodge JK, Heitman J, Davis RW, Fraser CM, Hyman RW
Science (New York, N.Y.)307(5713)1321-4(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CNA06050 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNA06050 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCn00646	XM_566959.2 Latest version!	Cryptococcus neoformans var. neoformans JEC21 ARF guanyl-nucleotide exchange factor, putative (CNA06050), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCn00646	XM_566959.1	Cryptococcus neoformans var. neoformans JEC21 ARF guanyl-nucleotide exchange factor, partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNA06050 ( XM_566959.2 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_566959.1 Cryptococcus neoformans var. neoformans JEC21 ARF guanyl-nucleotide exchange factor, putative (CNA06050), mRNA.

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CloneID	OCn00646
Clone ID Related Accession (Same CDS sequence)	XM_566959.2 , XM_566959.1
Accession Version	XM_566959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523289600000
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Product	ARF guanyl-nucleotide exchange factor, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006670). On Apr 11, 2018 this sequence version replaced XM_566959.1.

ATGGACAGTA ACGCAGAGGC AGGTCCCTCC ACACTGCGTC CACATCGATC ATCGCCGCCC 
TCGACACCGA CACCTTTAAC GCCTACGTCT GTTAGAGCGA AACGACGTTC ATGGTTCGGC 
TTGGCTTCTC CAGTGATCCC ACTTTCGAAA GAAAAGGCAA AAGATAAGCG CCGTGGAAGT 
TCTAACGGCA CTGAGGAATT AGAGCTCGGC CCAGTATCGA ACGGGCGGGA CAATGAGACC 
GTGAAAGGAG GTAGACGAGA GGATGCGCAC GAGGAAGAGC TGAGAGAGAG AACAGGGCAC 
GAAGGCGACG ATTTGCTAAC GATTGATGGG GATCTGGAAA AGACTGTTAA AAAGAAAAAG 
CGAAAGTCTG GTGGTGCCGA CGAGCCCCTG GTACTGAGGA TGGAAGATTT GGGTCATAAA 
ACACCTGCGA GAACGATGTC TGAAAAGACG TTAACGGTAA ACGATGTGAT GAAAACACCA 
CATGCGTTAA CTACATGGAC TTTCTCTCGC ACGGCGAGCG ACCGCGATCC TTTCAGCACC 
CCTGATCTAC CGTCAAATCC AGGACCCGAC AGCTCACTTT TCAACAAACT TGAGGAGGAG 
CTTCTCCTCA ACCCAGCACC AAGATCCTCA TCCCTAAATT CGGTACCATC TCCAATTACC 
TCTTCCCATA GAGATAAAAC TCAACCCCCT TTACCTGTCG GCATGGACAG ACCGTTGCCG 
CCGTTGCCGC CCCCAACACC TGATAGTAAG GGATTGAAAG AGCCTAAAGC TCCTTCCGAA 
ATTTTTATCA CCCCCAGCAG TCCACAAGAA CCCAAGGCTA CCAGAAGAGC ACATGAAGAG 
CGGCGTAGCA GGTCTATTTC GGGACGGCCC AGAAGCCATT CACGAGGCAG AGCTTCCAGC 
GCAGAGCCCT CAAAACCTCT TGGTTTGGGA TTACCTTCAG TCATCTCGGG AAGATCTCGG 
TCTGTATCGC GCTCACGGCC ATCTTCCAGA TCACCTTCTG TATCTGTTCA AAGCCCGTCC 
CCTCCGCCCG TTCCCTTAAA ACCGCAATCA GTATCGTCGC CATTTCCATC TCGTCCGGGG 
ACTCCCAATC ATAAAATTAC TTTTGCAGAG CCCGAAGTCA CAAAACCACA AGGTTTGCAA 
AAGGAAGATG AATCTGACAA AAAGACAAGA ATCAAAAGAG CGAGAAGTTT ATCGGGATTG 
TTTGGCAAAT CACCGCCAGT GGTATTATCC GCCAAAATGG GGGATCCCGT CCAAGATGAT 
GACCAGAAGA ATGGAGAAGA GGAGACAAAC GGTGATCAAG GAGGCGGCGG AAAGCTTGGG 
GTGCTAGAAT GGTTAGGAAT GAAAAAGACT GTGAGGCGAA AAGCTTCAGA GACGAGGCTC 
AGGGAACCGG CAAGTGAGCT GGATGACGTC CAGAACGTTG CTGGTGATAC ATCTAGATTC 
CCAGAAGATC AGGCTCCTAA CAAGGGAAAT ATAGACCTAG TCAGTGAGGG TGATCAGTAC 
AACGGACAAA CCGACCTTCC ACCCCCTCCT ACCACTAACG CACCACATGG TACTCCCAAG 
AGATTGAATA GCTTATTCGC TCGCCGATCA TCTGCGAAAA ACGAGGAAGA AGCAGAGCCG 
CTGTCAATTA TGGTTGTTCC TAGGGCTATT CATAGCTCTG AATGTTCGAC ACGCCCGCTG 
ACGAACGCTT CCCAATCTTC TCTGCAAGTT CCTGCCGTCG ATCCATTCTC ACCTGATGGA 
AGCAACACGT GGACATCCAG TCCAGGCATT GAGATTGAAG AGATGGTCTT CAGCCCTGGT 
AGTAGTGCTC ATTGGGGACC TGGGATACGC CCTTGGATGG AAACAAGGCA ACATAACGTC 
ACTTCGAGGT CGTCGTTATC TTGCGCTCTG GATACGCCAG TGCAAAAGTC CGAATCTTCC 
AAAGCGCCTG CTAAAGCACA GGGAGGGCGA GTCAGGTCAT TTTCTGATGG GCCTTTGCCA 
CAACAACCAC CCGATCACTC TCCAGCTCCC TCTCAACCTG CCTCAGATAG TCCTTCTCCC 
TCTCCCTCAA CGGGCCTCAA AACACCTGTA CGACCCAGAA TGGACAGCAG GACAGGCTCC 
AGCAACTCAG CGATCGTAGG GAGAATGAAA AGTGTGTTCT CCAAGTCCTC AAACCGGAGC 
AGGAGTAACA GTCTTTTACG CCAGGATGCC AGCGATGTTG ACGAGTTTGG GGGAGTGGCC 
AGCCAACGTA TGCGACTCTC GGCGTCGGCC TCTTCTATGA GGCCTTATGA TACCGCGGGA 
GGTCACGTCA TAATGGAGGA CAGCGTCCAT CTGGAATCAC AGCAGGAAAA AAATCACACA 
GTTGGAAGAG TGTCAAGTTC GTCAATGACA CCTTCTCTTT CATCCAATGC GTCTTCTACG 
AAACATTCAG TCCTCGGAGA TCCTCTTCAT ACCCACAAAA TAGCATCACG TCGAAGCAGA 
GCTCGAGCGT CAACTGTATC TCTTGCGCCA ACTTCGTACC ATTTTGCGCC GCCTTCGCCA 
AGCACTTTTC CCATCTCTGC AACGCCACCT CGCCGCCAAA GCACAATACG TCGTCTTTCT 
AATGGACTAT TTGGATCAGC ATCCTCGTCG CCCCAACATT CTGCTCTATT TCCTCTTCCG 
CCTAGATCCA ACGGATCTAC TTCATCAGTA GCTACAGGTG CGCAGATATC TGGTCAAGGC 
TGGGAGGATG GGTCCACTGG AGTGTTAAGT CCCGGTGCTA GCCCAAGGCC CAGCTTAGGT 
AGTCTAGCCG TTAAGACCAA TGCTATGAAA CAAGCTGATG TACCGGAAGG AGAAGAATCG 
CCCAGGGAAT GGTTGGAGAG GGCTCTCAGT GCTGTTGGGA GGTGCGAAAT AGCCAATGTG 
CTGGCGTCTA GCGGCGATGC TCTTCACGTG GAAGCATTAC AGATGTTCAT GTCAACGTTC 
AACTTCACTC ATAACGCTCT GGACGTCGCT TTGAGGAAAC TGCTTATGCA AATCTCTCTT 
CCGAAAGAGA CACAGCAGAT CGATCGTGTA ATTGAAGCGT TCGCAAAACA ATATGAAAGG 
TGTGAGCCCG GTCTCTTCGG CGAAAAAGAC AACGCATATG TCCTTGCATT CTCGATGATG 
ATGCTTCACA CCGATGCCTT CAATAAGCAT AACAAGAATA AGATGTCCAA GGCAGATTAT 
GTGCGCAATT CACGCATGGA AGGCGTGCCA TCTTTCGTGC TAGAAGCTTT CTATGACAAT 
ATCACGTTTA CTCCGTTTGT CTTCATTGAG GACGACTCTG ATCTGAAGCG CACATCTGGG 
CGTACAACTC CGTTGAATTT TGGGCCGTCT ACTCCGACGT TCTCTGCCTT CTTGAACGGC 
TCTAATCCTC CCAGCAAGTC CTCCAACAAA ATTGACGTGT ACGATCTTAT CGTGCGAGAT 
ATGCTTGATC AATTGAGAGT TGATGTGGAG AAGGAAATAC CGGCTGAAAA TCCATTTTCT 
TGTCTTGGAA CTAGACCAAT CCTTGATTTC GAAGGGTTGA GCAAGGCTTT TGCTTCTGCG 
CATAGCCTTT CAATTCCTGT ACCCCAGCAG AAGACAGCAA GGAGGAACAC ACTCATAACA 
TCAGGAAAGA AGCAAGCGGC GAAACAAGAG AAGGAGTTGG ACATGGCTCT GCGAGTAACA 
AAAGTCGGGC TAATATCTCG AAAAGATGAA GCTTTTAGCG AAACGGGCAA GAAAAATCGC 
AAATGGAAAT CTTGGAGCGT TATCCTCACT CAATCACAGC TACTTTTTTT TAAAGACCCT 
ATGTGGGCTT TGACACTGCT TGAGCAAGCT AGGGCGACAT CGGAAAATGA CAAGGATGGT 
CAACTGCTCC TCCCACGAAT GGCTTCTTTC AAACCAGATG AAGTATTCCC CGTCAAGGAC 
TGCATTGCAG TTTTCGACAA GAGTTTCACT GCTTATCCAA ACACCTTTCG TTTCGTTATC 
TCTCAGCACC AACAATATCT TATGCAAGCT CGGGACGAAC TTGAGATGAA TGAGTGGATC 
ACCCTCATCA ATTACGCCTC AGCTTTTAAA AGTGCCGGTA TCAAGATGCG ACGTGGAACA 
ATGCGCAAGG ACCAAGCGGT ATTGGCGGGC GCTGCTGCTG CTGCGTCTCA TAGAAGGGAC 
ATGCGTGAGG ACCGTTATGA CTCTTCGGAC GGAGCATCTA CCCCAGGACG AGTTGCTGTA 
TTTAGGGGCG TGGACCAAAC GAGTCAAGAA AAAACACCGG CCCACCTAAA AGGTGATGGT 
GTCAGAGGCG TAGATATTGA CGGTGCCAAC GACAGTTTGC AGGAAGGTGA GCAACTGGAA 
GAGGTATTCG GCGTCGTCAA AGCGGAGCTG GCGGCTGGTA GAGGAGGCGC AAAGACTGTG 
GGCACGGCTA GGCAGCCAGA TAGGTCTTAC AGCCATGCTT CCCGTTTAGC CATTATCCAG 
AACCATCTTC GAATTCTTCG TGAGAAGGTG GTACCGATTG AATCCTCCAT TCAATCCTCT 
CTCTTGCTTG CTCGCAACGT CAACATCCTA ACTCCTTTTC AAAAGTCTAC TCGTGACCGT 
CTTGCTTCCA CTATTCCTTC ATTGGCTCGG CGCACCAGGA CTGAACGCAT CTTCCTCTCA 
AAGGTGCAAT TTTGGATTAA TGTCCTCGAG TATGAAGCGG ATCGCGCTGA ACGAGAGTGG 
AAAGAAGTGC GACACCTGGC ATTACAGGCA GCAGCAAGAA GTATGCGTGA AGATGGAGTT 
AGTGGCGTTG TTGAGGACGT CGCAAGAGAT GAGGAGGGCG GAGATCGCGG CGTACCAGTC 
CTGTCTCTCC CTAACAGCGG GGATGAAGAT GTGGAAAACG AGGCAACGCA TTTGAGCATG 
AGCCCAGGAG AACTGCCAAT CATGTTTCGA AGACCAAGCA ATGGACCCGA TAATGCTAGG 
TATCCTACTT CTCGTGATAC AAGCTCTAGT CGCGTTGCCA GCCTCCTTGA AGAAAGGGGA 
GGGGATAGAC GTCAAACTGT TTCATCCGAC TACTGGCTCA GTCCACATGA GACTGAACGG 
CTGTCGAGTG ATGTGGAAAG TGCCCATTCA AGAACCGGTA CTCCAGACAT GGGTCTTAAC 
GGATATGACG GCTCAAGGAG GGAGCGAAAA GATAGCGAAG CAGGGCGATC GAGTGGTCGA 
AACACGCCAA TGATGTTTGT CATGGAAAGC CCGATGGAAA TTGGATACGA AGAGATAAAC 
CATGAAAGGT TTTTCGAGTA TACACCTGTC AACCCCCAGA ATGGAAAAGA GACTGGGAAG 
AAGAATGAAT CGAGAGACAG TTGTGAGGAA GGAAAGAAAG AAGAGGCGGA GGATTGGCAA 
GATACGAAAG CTGCGAAAAA AGTGTCGCTA GTGCGGCCAG GCGAGATGAG TGATTGGGAG 
AGCATGCGCA AAGTCAGCCC TGGAAACAAA GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_566959.1
CDS	88..5523
Translation	MDSNAEAGPSTLRPHRSSPPSTPTPLTPTSVRAKRRSWFGLASPVIPLSKEKAKDKRRGSSNGTEELELGPVSNGRDNETVKGGRREDAHEEELRERTGHEGDDLLTIDGDLEKTVKKKKRKSGGADEPLVLRMEDLGHKTPARTMSEKTLTVNDVMKTPHALTTWTFSRTASDRDPFSTPDLPSNPGPDSSLFNKLEEELLLNPAPRSSSLNSVPSPITSSHRDKTQPPLPVGMDRPLPPLPPPTPDSKGLKEPKAPSEIFITPSSPQEPKATRRAHEERRSRSISGRPRSHSRGRASSAEPSKPLGLGLPSVISGRSRSVSRSRPSSRSPSVSVQSPSPPPVPLKPQSVSSPFPSRPGTPNHKITFAEPEVTKPQGLQKEDESDKKTRIKRARSLSGLFGKSPPVVLSAKMGDPVQDDDQKNGEEETNGDQGGGGKLGVLEWLGMKKTVRRKASETRLREPASELDDVQNVAGDTSRFPEDQAPNKGNIDLVSEGDQYNGQTDLPPPPTTNAPHGTPKRLNSLFARRSSAKNEEEAEPLSIMVVPRAIHSSECSTRPLTNASQSSLQVPAVDPFSPDGSNTWTSSPGIEIEEMVFSPGSSAHWGPGIRPWMETRQHNVTSRSSLSCALDTPVQKSESSKAPAKAQGGRVRSFSDGPLPQQPPDHSPAPSQPASDSPSPSPSTGLKTPVRPRMDSRTGSSNSAIVGRMKSVFSKSSNRSRSNSLLRQDASDVDEFGGVASQRMRLSASASSMRPYDTAGGHVIMEDSVHLESQQEKNHTVGRVSSSSMTPSLSSNASSTKHSVLGDPLHTHKIASRRSRARASTVSLAPTSYHFAPPSPSTFPISATPPRRQSTIRRLSNGLFGSASSSPQHSALFPLPPRSNGSTSSVATGAQISGQGWEDGSTGVLSPGASPRPSLGSLAVKTNAMKQADVPEGEESPREWLERALSAVGRCEIANVLASSGDALHVEALQMFMSTFNFTHNALDVALRKLLMQISLPKETQQIDRVIEAFAKQYERCEPGLFGEKDNAYVLAFSMMMLHTDAFNKHNKNKMSKADYVRNSRMEGVPSFVLEAFYDNITFTPFVFIEDDSDLKRTSGRTTPLNFGPSTPTFSAFLNGSNPPSKSSNKIDVYDLIVRDMLDQLRVDVEKEIPAENPFSCLGTRPILDFEGLSKAFASAHSLSIPVPQQKTARRNTLITSGKKQAAKQEKELDMALRVTKVGLISRKDEAFSETGKKNRKWKSWSVILTQSQLLFFKDPMWALTLLEQARATSENDKDGQLLLPRMASFKPDEVFPVKDCIAVFDKSFTAYPNTFRFVISQHQQYLMQARDELEMNEWITLINYASAFKSAGIKMRRGTMRKDQAVLAGAAAAASHRRDMREDRYDSSDGASTPGRVAVFRGVDQTSQEKTPAHLKGDGVRGVDIDGANDSLQEGEQLEEVFGVVKAELAAGRGGAKTVGTARQPDRSYSHASRLAIIQNHLRILREKVVPIESSIQSSLLLARNVNILTPFQKSTRDRLASTIPSLARRTRTERIFLSKVQFWINVLEYEADRAEREWKEVRHLALQAAARSMREDGVSGVVEDVARDEEGGDRGVPVLSLPNSGDEDVENEATHLSMSPGELPIMFRRPSNGPDNARYPTSRDTSSSRVASLLEERGGDRRQTVSSDYWLSPHETERLSSDVESAHSRTGTPDMGLNGYDGSRRERKDSEAGRSSGRNTPMMFVMESPMEIGYEEINHERFFEYTPVNPQNGKETGKKNESRDSCEEGKKEEAEDWQDTKAAKKVSLVRPGEMSDWESMRKVSPGNKG

Target ORF information:

RefSeq Version	XM_566959.2
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Definition	Cryptococcus neoformans var. neoformans JEC21 ARF guanyl-nucleotide exchange factor, putative (CNA06050), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_566959.2

ORF Insert Sequence:

ATGGACAGTA ACGCAGAGGC AGGTCCCTCC ACACTGCGTC CACATCGATC ATCGCCGCCC 
TCGACACCGA CACCTTTAAC GCCTACGTCT GTTAGAGCGA AACGACGTTC ATGGTTCGGC 
TTGGCTTCTC CAGTGATCCC ACTTTCGAAA GAAAAGGCAA AAGATAAGCG CCGTGGAAGT 
TCTAACGGCA CTGAGGAATT AGAGCTCGGC CCAGTATCGA ACGGGCGGGA CAATGAGACC 
GTGAAAGGAG GTAGACGAGA GGATGCGCAC GAGGAAGAGC TGAGAGAGAG AACAGGGCAC 
GAAGGCGACG ATTTGCTAAC GATTGATGGG GATCTGGAAA AGACTGTTAA AAAGAAAAAG 
CGAAAGTCTG GTGGTGCCGA CGAGCCCCTG GTACTGAGGA TGGAAGATTT GGGTCATAAA 
ACACCTGCGA GAACGATGTC TGAAAAGACG TTAACGGTAA ACGATGTGAT GAAAACACCA 
CATGCGTTAA CTACATGGAC TTTCTCTCGC ACGGCGAGCG ACCGCGATCC TTTCAGCACC 
CCTGATCTAC CGTCAAATCC AGGACCCGAC AGCTCACTTT TCAACAAACT TGAGGAGGAG 
CTTCTCCTCA ACCCAGCACC AAGATCCTCA TCCCTAAATT CGGTACCATC TCCAATTACC 
TCTTCCCATA GAGATAAAAC TCAACCCCCT TTACCTGTCG GCATGGACAG ACCGTTGCCG 
CCGTTGCCGC CCCCAACACC TGATAGTAAG GGATTGAAAG AGCCTAAAGC TCCTTCCGAA 
ATTTTTATCA CCCCCAGCAG TCCACAAGAA CCCAAGGCTA CCAGAAGAGC ACATGAAGAG 
CGGCGTAGCA GGTCTATTTC GGGACGGCCC AGAAGCCATT CACGAGGCAG AGCTTCCAGC 
GCAGAGCCCT CAAAACCTCT TGGTTTGGGA TTACCTTCAG TCATCTCGGG AAGATCTCGG 
TCTGTATCGC GCTCACGGCC ATCTTCCAGA TCACCTTCTG TATCTGTTCA AAGCCCGTCC 
CCTCCGCCCG TTCCCTTAAA ACCGCAATCA GTATCGTCGC CATTTCCATC TCGTCCGGGG 
ACTCCCAATC ATAAAATTAC TTTTGCAGAG CCCGAAGTCA CAAAACCACA AGGTTTGCAA 
AAGGAAGATG AATCTGACAA AAAGACAAGA ATCAAAAGAG CGAGAAGTTT ATCGGGATTG 
TTTGGCAAAT CACCGCCAGT GGTATTATCC GCCAAAATGG GGGATCCCGT CCAAGATGAT 
GACCAGAAGA ATGGAGAAGA GGAGACAAAC GGTGATCAAG GAGGCGGCGG AAAGCTTGGG 
GTGCTAGAAT GGTTAGGAAT GAAAAAGACT GTGAGGCGAA AAGCTTCAGA GACGAGGCTC 
AGGGAACCGG CAAGTGAGCT GGATGACGTC CAGAACGTTG CTGGTGATAC ATCTAGATTC 
CCAGAAGATC AGGCTCCTAA CAAGGGAAAT ATAGACCTAG TCAGTGAGGG TGATCAGTAC 
AACGGACAAA CCGACCTTCC ACCCCCTCCT ACCACTAACG CACCACATGG TACTCCCAAG 
AGATTGAATA GCTTATTCGC TCGCCGATCA TCTGCGAAAA ACGAGGAAGA AGCAGAGCCG 
CTGTCAATTA TGGTTGTTCC TAGGGCTATT CATAGCTCTG AATGTTCGAC ACGCCCGCTG 
ACGAACGCTT CCCAATCTTC TCTGCAAGTT CCTGCCGTCG ATCCATTCTC ACCTGATGGA 
AGCAACACGT GGACATCCAG TCCAGGCATT GAGATTGAAG AGATGGTCTT CAGCCCTGGT 
AGTAGTGCTC ATTGGGGACC TGGGATACGC CCTTGGATGG AAACAAGGCA ACATAACGTC 
ACTTCGAGGT CGTCGTTATC TTGCGCTCTG GATACGCCAG TGCAAAAGTC CGAATCTTCC 
AAAGCGCCTG CTAAAGCACA GGGAGGGCGA GTCAGGTCAT TTTCTGATGG GCCTTTGCCA 
CAACAACCAC CCGATCACTC TCCAGCTCCC TCTCAACCTG CCTCAGATAG TCCTTCTCCC 
TCTCCCTCAA CGGGCCTCAA AACACCTGTA CGACCCAGAA TGGACAGCAG GACAGGCTCC 
AGCAACTCAG CGATCGTAGG GAGAATGAAA AGTGTGTTCT CCAAGTCCTC AAACCGGAGC 
AGGAGTAACA GTCTTTTACG CCAGGATGCC AGCGATGTTG ACGAGTTTGG GGGAGTGGCC 
AGCCAACGTA TGCGACTCTC GGCGTCGGCC TCTTCTATGA GGCCTTATGA TACCGCGGGA 
GGTCACGTCA TAATGGAGGA CAGCGTCCAT CTGGAATCAC AGCAGGAAAA AAATCACACA 
GTTGGAAGAG TGTCAAGTTC GTCAATGACA CCTTCTCTTT CATCCAATGC GTCTTCTACG 
AAACATTCAG TCCTCGGAGA TCCTCTTCAT ACCCACAAAA TAGCATCACG TCGAAGCAGA 
GCTCGAGCGT CAACTGTATC TCTTGCGCCA ACTTCGTACC ATTTTGCGCC GCCTTCGCCA 
AGCACTTTTC CCATCTCTGC AACGCCACCT CGCCGCCAAA GCACAATACG TCGTCTTTCT 
AATGGACTAT TTGGATCAGC ATCCTCGTCG CCCCAACATT CTGCTCTATT TCCTCTTCCG 
CCTAGATCCA ACGGATCTAC TTCATCAGTA GCTACAGGTG CGCAGATATC TGGTCAAGGC 
TGGGAGGATG GGTCCACTGG AGTGTTAAGT CCCGGTGCTA GCCCAAGGCC CAGCTTAGGT 
AGTCTAGCCG TTAAGACCAA TGCTATGAAA CAAGCTGATG TACCGGAAGG AGAAGAATCG 
CCCAGGGAAT GGTTGGAGAG GGCTCTCAGT GCTGTTGGGA GGTGCGAAAT AGCCAATGTG 
CTGGCGTCTA GCGGCGATGC TCTTCACGTG GAAGCATTAC AGATGTTCAT GTCAACGTTC 
AACTTCACTC ATAACGCTCT GGACGTCGCT TTGAGGAAAC TGCTTATGCA AATCTCTCTT 
CCGAAAGAGA CACAGCAGAT CGATCGTGTA ATTGAAGCGT TCGCAAAACA ATATGAAAGG 
TGTGAGCCCG GTCTCTTCGG CGAAAAAGAC AACGCATATG TCCTTGCATT CTCGATGATG 
ATGCTTCACA CCGATGCCTT CAATAAGCAT AACAAGAATA AGATGTCCAA GGCAGATTAT 
GTGCGCAATT CACGCATGGA AGGCGTGCCA TCTTTCGTGC TAGAAGCTTT CTATGACAAT 
ATCACGTTTA CTCCGTTTGT CTTCATTGAG GACGACTCTG ATCTGAAGCG CACATCTGGG 
CGTACAACTC CGTTGAATTT TGGGCCGTCT ACTCCGACGT TCTCTGCCTT CTTGAACGGC 
TCTAATCCTC CCAGCAAGTC CTCCAACAAA ATTGACGTGT ACGATCTTAT CGTGCGAGAT 
ATGCTTGATC AATTGAGAGT TGATGTGGAG AAGGAAATAC CGGCTGAAAA TCCATTTTCT 
TGTCTTGGAA CTAGACCAAT CCTTGATTTC GAAGGGTTGA GCAAGGCTTT TGCTTCTGCG 
CATAGCCTTT CAATTCCTGT ACCCCAGCAG AAGACAGCAA GGAGGAACAC ACTCATAACA 
TCAGGAAAGA AGCAAGCGGC GAAACAAGAG AAGGAGTTGG ACATGGCTCT GCGAGTAACA 
AAAGTCGGGC TAATATCTCG AAAAGATGAA GCTTTTAGCG AAACGGGCAA GAAAAATCGC 
AAATGGAAAT CTTGGAGCGT TATCCTCACT CAATCACAGC TACTTTTTTT TAAAGACCCT 
ATGTGGGCTT TGACACTGCT TGAGCAAGCT AGGGCGACAT CGGAAAATGA CAAGGATGGT 
CAACTGCTCC TCCCACGAAT GGCTTCTTTC AAACCAGATG AAGTATTCCC CGTCAAGGAC 
TGCATTGCAG TTTTCGACAA GAGTTTCACT GCTTATCCAA ACACCTTTCG TTTCGTTATC 
TCTCAGCACC AACAATATCT TATGCAAGCT CGGGACGAAC TTGAGATGAA TGAGTGGATC 
ACCCTCATCA ATTACGCCTC AGCTTTTAAA AGTGCCGGTA TCAAGATGCG ACGTGGAACA 
ATGCGCAAGG ACCAAGCGGT ATTGGCGGGC GCTGCTGCTG CTGCGTCTCA TAGAAGGGAC 
ATGCGTGAGG ACCGTTATGA CTCTTCGGAC GGAGCATCTA CCCCAGGACG AGTTGCTGTA 
TTTAGGGGCG TGGACCAAAC GAGTCAAGAA AAAACACCGG CCCACCTAAA AGGTGATGGT 
GTCAGAGGCG TAGATATTGA CGGTGCCAAC GACAGTTTGC AGGAAGGTGA GCAACTGGAA 
GAGGTATTCG GCGTCGTCAA AGCGGAGCTG GCGGCTGGTA GAGGAGGCGC AAAGACTGTG 
GGCACGGCTA GGCAGCCAGA TAGGTCTTAC AGCCATGCTT CCCGTTTAGC CATTATCCAG 
AACCATCTTC GAATTCTTCG TGAGAAGGTG GTACCGATTG AATCCTCCAT TCAATCCTCT 
CTCTTGCTTG CTCGCAACGT CAACATCCTA ACTCCTTTTC AAAAGTCTAC TCGTGACCGT 
CTTGCTTCCA CTATTCCTTC ATTGGCTCGG CGCACCAGGA CTGAACGCAT CTTCCTCTCA 
AAGGTGCAAT TTTGGATTAA TGTCCTCGAG TATGAAGCGG ATCGCGCTGA ACGAGAGTGG 
AAAGAAGTGC GACACCTGGC ATTACAGGCA GCAGCAAGAA GTATGCGTGA AGATGGAGTT 
AGTGGCGTTG TTGAGGACGT CGCAAGAGAT GAGGAGGGCG GAGATCGCGG CGTACCAGTC 
CTGTCTCTCC CTAACAGCGG GGATGAAGAT GTGGAAAACG AGGCAACGCA TTTGAGCATG 
AGCCCAGGAG AACTGCCAAT CATGTTTCGA AGACCAAGCA ATGGACCCGA TAATGCTAGG 
TATCCTACTT CTCGTGATAC AAGCTCTAGT CGCGTTGCCA GCCTCCTTGA AGAAAGGGGA 
GGGGATAGAC GTCAAACTGT TTCATCCGAC TACTGGCTCA GTCCACATGA GACTGAACGG 
CTGTCGAGTG ATGTGGAAAG TGCCCATTCA AGAACCGGTA CTCCAGACAT GGGTCTTAAC 
GGATATGACG GCTCAAGGAG GGAGCGAAAA GATAGCGAAG CAGGGCGATC GAGTGGTCGA 
AACACGCCAA TGATGTTTGT CATGGAAAGC CCGATGGAAA TTGGATACGA AGAGATAAAC 
CATGAAAGGT TTTTCGAGTA TACACCTGTC AACCCCCAGA ATGGAAAAGA GACTGGGAAG 
AAGAATGAAT CGAGAGACAG TTGTGAGGAA GGAAAGAAAG AAGAGGCGGA GGATTGGCAA 
GATACGAAAG CTGCGAAAAA AGTGTCGCTA GTGCGGCCAG GCGAGATGAG TGATTGGGAG 
AGCATGCGCA AAGTCAGCCC TGGAAACAAA GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNA06050 ( XM_566959.1 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_566959.1 Cryptococcus neoformans var. neoformans JEC21 ARF guanyl-nucleotide exchange factor, partial mRNA.

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CloneID	OCn00646
Clone ID Related Accession (Same CDS sequence)	XM_566959.2 , XM_566959.1
Accession Version	XM_566959.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1345046400000
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Product	ARF guanyl-nucleotide exchange factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006670). The reference sequence was derived from mrna.CNA06050.1. This record represents the complete annotated sequence of the C. neoformans JEC21 genome. A small number of gaps exist which are represented by NNNNs within the final sequence. The genome has been annotated by TIGR and the annotation data is available for viewing and download from the TIGR database (http://www.tigr.org/tdb/e2k1/cna1/).

ATGGACAGTA ACGCAGAGGC AGGTCCCTCC ACACTGCGTC CACATCGATC ATCGCCGCCC 
TCGACACCGA CACCTTTAAC GCCTACGTCT GTTAGAGCGA AACGACGTTC ATGGTTCGGC 
TTGGCTTCTC CAGTGATCCC ACTTTCGAAA GAAAAGGCAA AAGATAAGCG CCGTGGAAGT 
TCTAACGGCA CTGAGGAATT AGAGCTCGGC CCAGTATCGA ACGGGCGGGA CAATGAGACC 
GTGAAAGGAG GTAGACGAGA GGATGCGCAC GAGGAAGAGC TGAGAGAGAG AACAGGGCAC 
GAAGGCGACG ATTTGCTAAC GATTGATGGG GATCTGGAAA AGACTGTTAA AAAGAAAAAG 
CGAAAGTCTG GTGGTGCCGA CGAGCCCCTG GTACTGAGGA TGGAAGATTT GGGTCATAAA 
ACACCTGCGA GAACGATGTC TGAAAAGACG TTAACGGTAA ACGATGTGAT GAAAACACCA 
CATGCGTTAA CTACATGGAC TTTCTCTCGC ACGGCGAGCG ACCGCGATCC TTTCAGCACC 
CCTGATCTAC CGTCAAATCC AGGACCCGAC AGCTCACTTT TCAACAAACT TGAGGAGGAG 
CTTCTCCTCA ACCCAGCACC AAGATCCTCA TCCCTAAATT CGGTACCATC TCCAATTACC 
TCTTCCCATA GAGATAAAAC TCAACCCCCT TTACCTGTCG GCATGGACAG ACCGTTGCCG 
CCGTTGCCGC CCCCAACACC TGATAGTAAG GGATTGAAAG AGCCTAAAGC TCCTTCCGAA 
ATTTTTATCA CCCCCAGCAG TCCACAAGAA CCCAAGGCTA CCAGAAGAGC ACATGAAGAG 
CGGCGTAGCA GGTCTATTTC GGGACGGCCC AGAAGCCATT CACGAGGCAG AGCTTCCAGC 
GCAGAGCCCT CAAAACCTCT TGGTTTGGGA TTACCTTCAG TCATCTCGGG AAGATCTCGG 
TCTGTATCGC GCTCACGGCC ATCTTCCAGA TCACCTTCTG TATCTGTTCA AAGCCCGTCC 
CCTCCGCCCG TTCCCTTAAA ACCGCAATCA GTATCGTCGC CATTTCCATC TCGTCCGGGG 
ACTCCCAATC ATAAAATTAC TTTTGCAGAG CCCGAAGTCA CAAAACCACA AGGTTTGCAA 
AAGGAAGATG AATCTGACAA AAAGACAAGA ATCAAAAGAG CGAGAAGTTT ATCGGGATTG 
TTTGGCAAAT CACCGCCAGT GGTATTATCC GCCAAAATGG GGGATCCCGT CCAAGATGAT 
GACCAGAAGA ATGGAGAAGA GGAGACAAAC GGTGATCAAG GAGGCGGCGG AAAGCTTGGG 
GTGCTAGAAT GGTTAGGAAT GAAAAAGACT GTGAGGCGAA AAGCTTCAGA GACGAGGCTC 
AGGGAACCGG CAAGTGAGCT GGATGACGTC CAGAACGTTG CTGGTGATAC ATCTAGATTC 
CCAGAAGATC AGGCTCCTAA CAAGGGAAAT ATAGACCTAG TCAGTGAGGG TGATCAGTAC 
AACGGACAAA CCGACCTTCC ACCCCCTCCT ACCACTAACG CACCACATGG TACTCCCAAG 
AGATTGAATA GCTTATTCGC TCGCCGATCA TCTGCGAAAA ACGAGGAAGA AGCAGAGCCG 
CTGTCAATTA TGGTTGTTCC TAGGGCTATT CATAGCTCTG AATGTTCGAC ACGCCCGCTG 
ACGAACGCTT CCCAATCTTC TCTGCAAGTT CCTGCCGTCG ATCCATTCTC ACCTGATGGA 
AGCAACACGT GGACATCCAG TCCAGGCATT GAGATTGAAG AGATGGTCTT CAGCCCTGGT 
AGTAGTGCTC ATTGGGGACC TGGGATACGC CCTTGGATGG AAACAAGGCA ACATAACGTC 
ACTTCGAGGT CGTCGTTATC TTGCGCTCTG GATACGCCAG TGCAAAAGTC CGAATCTTCC 
AAAGCGCCTG CTAAAGCACA GGGAGGGCGA GTCAGGTCAT TTTCTGATGG GCCTTTGCCA 
CAACAACCAC CCGATCACTC TCCAGCTCCC TCTCAACCTG CCTCAGATAG TCCTTCTCCC 
TCTCCCTCAA CGGGCCTCAA AACACCTGTA CGACCCAGAA TGGACAGCAG GACAGGCTCC 
AGCAACTCAG CGATCGTAGG GAGAATGAAA AGTGTGTTCT CCAAGTCCTC AAACCGGAGC 
AGGAGTAACA GTCTTTTACG CCAGGATGCC AGCGATGTTG ACGAGTTTGG GGGAGTGGCC 
AGCCAACGTA TGCGACTCTC GGCGTCGGCC TCTTCTATGA GGCCTTATGA TACCGCGGGA 
GGTCACGTCA TAATGGAGGA CAGCGTCCAT CTGGAATCAC AGCAGGAAAA AAATCACACA 
GTTGGAAGAG TGTCAAGTTC GTCAATGACA CCTTCTCTTT CATCCAATGC GTCTTCTACG 
AAACATTCAG TCCTCGGAGA TCCTCTTCAT ACCCACAAAA TAGCATCACG TCGAAGCAGA 
GCTCGAGCGT CAACTGTATC TCTTGCGCCA ACTTCGTACC ATTTTGCGCC GCCTTCGCCA 
AGCACTTTTC CCATCTCTGC AACGCCACCT CGCCGCCAAA GCACAATACG TCGTCTTTCT 
AATGGACTAT TTGGATCAGC ATCCTCGTCG CCCCAACATT CTGCTCTATT TCCTCTTCCG 
CCTAGATCCA ACGGATCTAC TTCATCAGTA GCTACAGGTG CGCAGATATC TGGTCAAGGC 
TGGGAGGATG GGTCCACTGG AGTGTTAAGT CCCGGTGCTA GCCCAAGGCC CAGCTTAGGT 
AGTCTAGCCG TTAAGACCAA TGCTATGAAA CAAGCTGATG TACCGGAAGG AGAAGAATCG 
CCCAGGGAAT GGTTGGAGAG GGCTCTCAGT GCTGTTGGGA GGTGCGAAAT AGCCAATGTG 
CTGGCGTCTA GCGGCGATGC TCTTCACGTG GAAGCATTAC AGATGTTCAT GTCAACGTTC 
AACTTCACTC ATAACGCTCT GGACGTCGCT TTGAGGAAAC TGCTTATGCA AATCTCTCTT 
CCGAAAGAGA CACAGCAGAT CGATCGTGTA ATTGAAGCGT TCGCAAAACA ATATGAAAGG 
TGTGAGCCCG GTCTCTTCGG CGAAAAAGAC AACGCATATG TCCTTGCATT CTCGATGATG 
ATGCTTCACA CCGATGCCTT CAATAAGCAT AACAAGAATA AGATGTCCAA GGCAGATTAT 
GTGCGCAATT CACGCATGGA AGGCGTGCCA TCTTTCGTGC TAGAAGCTTT CTATGACAAT 
ATCACGTTTA CTCCGTTTGT CTTCATTGAG GACGACTCTG ATCTGAAGCG CACATCTGGG 
CGTACAACTC CGTTGAATTT TGGGCCGTCT ACTCCGACGT TCTCTGCCTT CTTGAACGGC 
TCTAATCCTC CCAGCAAGTC CTCCAACAAA ATTGACGTGT ACGATCTTAT CGTGCGAGAT 
ATGCTTGATC AATTGAGAGT TGATGTGGAG AAGGAAATAC CGGCTGAAAA TCCATTTTCT 
TGTCTTGGAA CTAGACCAAT CCTTGATTTC GAAGGGTTGA GCAAGGCTTT TGCTTCTGCG 
CATAGCCTTT CAATTCCTGT ACCCCAGCAG AAGACAGCAA GGAGGAACAC ACTCATAACA 
TCAGGAAAGA AGCAAGCGGC GAAACAAGAG AAGGAGTTGG ACATGGCTCT GCGAGTAACA 
AAAGTCGGGC TAATATCTCG AAAAGATGAA GCTTTTAGCG AAACGGGCAA GAAAAATCGC 
AAATGGAAAT CTTGGAGCGT TATCCTCACT CAATCACAGC TACTTTTTTT TAAAGACCCT 
ATGTGGGCTT TGACACTGCT TGAGCAAGCT AGGGCGACAT CGGAAAATGA CAAGGATGGT 
CAACTGCTCC TCCCACGAAT GGCTTCTTTC AAACCAGATG AAGTATTCCC CGTCAAGGAC 
TGCATTGCAG TTTTCGACAA GAGTTTCACT GCTTATCCAA ACACCTTTCG TTTCGTTATC 
TCTCAGCACC AACAATATCT TATGCAAGCT CGGGACGAAC TTGAGATGAA TGAGTGGATC 
ACCCTCATCA ATTACGCCTC AGCTTTTAAA AGTGCCGGTA TCAAGATGCG ACGTGGAACA 
ATGCGCAAGG ACCAAGCGGT ATTGGCGGGC GCTGCTGCTG CTGCGTCTCA TAGAAGGGAC 
ATGCGTGAGG ACCGTTATGA CTCTTCGGAC GGAGCATCTA CCCCAGGACG AGTTGCTGTA 
TTTAGGGGCG TGGACCAAAC GAGTCAAGAA AAAACACCGG CCCACCTAAA AGGTGATGGT 
GTCAGAGGCG TAGATATTGA CGGTGCCAAC GACAGTTTGC AGGAAGGTGA GCAACTGGAA 
GAGGTATTCG GCGTCGTCAA AGCGGAGCTG GCGGCTGGTA GAGGAGGCGC AAAGACTGTG 
GGCACGGCTA GGCAGCCAGA TAGGTCTTAC AGCCATGCTT CCCGTTTAGC CATTATCCAG 
AACCATCTTC GAATTCTTCG TGAGAAGGTG GTACCGATTG AATCCTCCAT TCAATCCTCT 
CTCTTGCTTG CTCGCAACGT CAACATCCTA ACTCCTTTTC AAAAGTCTAC TCGTGACCGT 
CTTGCTTCCA CTATTCCTTC ATTGGCTCGG CGCACCAGGA CTGAACGCAT CTTCCTCTCA 
AAGGTGCAAT TTTGGATTAA TGTCCTCGAG TATGAAGCGG ATCGCGCTGA ACGAGAGTGG 
AAAGAAGTGC GACACCTGGC ATTACAGGCA GCAGCAAGAA GTATGCGTGA AGATGGAGTT 
AGTGGCGTTG TTGAGGACGT CGCAAGAGAT GAGGAGGGCG GAGATCGCGG CGTACCAGTC 
CTGTCTCTCC CTAACAGCGG GGATGAAGAT GTGGAAAACG AGGCAACGCA TTTGAGCATG 
AGCCCAGGAG AACTGCCAAT CATGTTTCGA AGACCAAGCA ATGGACCCGA TAATGCTAGG 
TATCCTACTT CTCGTGATAC AAGCTCTAGT CGCGTTGCCA GCCTCCTTGA AGAAAGGGGA 
GGGGATAGAC GTCAAACTGT TTCATCCGAC TACTGGCTCA GTCCACATGA GACTGAACGG 
CTGTCGAGTG ATGTGGAAAG TGCCCATTCA AGAACCGGTA CTCCAGACAT GGGTCTTAAC 
GGATATGACG GCTCAAGGAG GGAGCGAAAA GATAGCGAAG CAGGGCGATC GAGTGGTCGA 
AACACGCCAA TGATGTTTGT CATGGAAAGC CCGATGGAAA TTGGATACGA AGAGATAAAC 
CATGAAAGGT TTTTCGAGTA TACACCTGTC AACCCCCAGA ATGGAAAAGA GACTGGGAAG 
AAGAATGAAT CGAGAGACAG TTGTGAGGAA GGAAAGAAAG AAGAGGCGGA GGATTGGCAA 
GATACGAAAG CTGCGAAAAA AGTGTCGCTA GTGCGGCCAG GCGAGATGAG TGATTGGGAG 
AGCATGCGCA AAGTCAGCCC TGGAAACAAA GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_566959.1
CDS	78..5513
Translation	MDSNAEAGPSTLRPHRSSPPSTPTPLTPTSVRAKRRSWFGLASPVIPLSKEKAKDKRRGSSNGTEELELGPVSNGRDNETVKGGRREDAHEEELRERTGHEGDDLLTIDGDLEKTVKKKKRKSGGADEPLVLRMEDLGHKTPARTMSEKTLTVNDVMKTPHALTTWTFSRTASDRDPFSTPDLPSNPGPDSSLFNKLEEELLLNPAPRSSSLNSVPSPITSSHRDKTQPPLPVGMDRPLPPLPPPTPDSKGLKEPKAPSEIFITPSSPQEPKATRRAHEERRSRSISGRPRSHSRGRASSAEPSKPLGLGLPSVISGRSRSVSRSRPSSRSPSVSVQSPSPPPVPLKPQSVSSPFPSRPGTPNHKITFAEPEVTKPQGLQKEDESDKKTRIKRARSLSGLFGKSPPVVLSAKMGDPVQDDDQKNGEEETNGDQGGGGKLGVLEWLGMKKTVRRKASETRLREPASELDDVQNVAGDTSRFPEDQAPNKGNIDLVSEGDQYNGQTDLPPPPTTNAPHGTPKRLNSLFARRSSAKNEEEAEPLSIMVVPRAIHSSECSTRPLTNASQSSLQVPAVDPFSPDGSNTWTSSPGIEIEEMVFSPGSSAHWGPGIRPWMETRQHNVTSRSSLSCALDTPVQKSESSKAPAKAQGGRVRSFSDGPLPQQPPDHSPAPSQPASDSPSPSPSTGLKTPVRPRMDSRTGSSNSAIVGRMKSVFSKSSNRSRSNSLLRQDASDVDEFGGVASQRMRLSASASSMRPYDTAGGHVIMEDSVHLESQQEKNHTVGRVSSSSMTPSLSSNASSTKHSVLGDPLHTHKIASRRSRARASTVSLAPTSYHFAPPSPSTFPISATPPRRQSTIRRLSNGLFGSASSSPQHSALFPLPPRSNGSTSSVATGAQISGQGWEDGSTGVLSPGASPRPSLGSLAVKTNAMKQADVPEGEESPREWLERALSAVGRCEIANVLASSGDALHVEALQMFMSTFNFTHNALDVALRKLLMQISLPKETQQIDRVIEAFAKQYERCEPGLFGEKDNAYVLAFSMMMLHTDAFNKHNKNKMSKADYVRNSRMEGVPSFVLEAFYDNITFTPFVFIEDDSDLKRTSGRTTPLNFGPSTPTFSAFLNGSNPPSKSSNKIDVYDLIVRDMLDQLRVDVEKEIPAENPFSCLGTRPILDFEGLSKAFASAHSLSIPVPQQKTARRNTLITSGKKQAAKQEKELDMALRVTKVGLISRKDEAFSETGKKNRKWKSWSVILTQSQLLFFKDPMWALTLLEQARATSENDKDGQLLLPRMASFKPDEVFPVKDCIAVFDKSFTAYPNTFRFVISQHQQYLMQARDELEMNEWITLINYASAFKSAGIKMRRGTMRKDQAVLAGAAAAASHRRDMREDRYDSSDGASTPGRVAVFRGVDQTSQEKTPAHLKGDGVRGVDIDGANDSLQEGEQLEEVFGVVKAELAAGRGGAKTVGTARQPDRSYSHASRLAIIQNHLRILREKVVPIESSIQSSLLLARNVNILTPFQKSTRDRLASTIPSLARRTRTERIFLSKVQFWINVLEYEADRAEREWKEVRHLALQAAARSMREDGVSGVVEDVARDEEGGDRGVPVLSLPNSGDEDVENEATHLSMSPGELPIMFRRPSNGPDNARYPTSRDTSSSRVASLLEERGGDRRQTVSSDYWLSPHETERLSSDVESAHSRTGTPDMGLNGYDGSRRERKDSEAGRSSGRNTPMMFVMESPMEIGYEEINHERFFEYTPVNPQNGKETGKKNESRDSCEEGKKEEAEDWQDTKAAKKVSLVRPGEMSDWESMRKVSPGNKG

Target ORF information:

RefSeq Version	XM_566959.1
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Definition	Cryptococcus neoformans var. neoformans JEC21 ARF guanyl-nucleotide exchange factor, partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_566959.1

ORF Insert Sequence:

ATGGACAGTA ACGCAGAGGC AGGTCCCTCC ACACTGCGTC CACATCGATC ATCGCCGCCC 
TCGACACCGA CACCTTTAAC GCCTACGTCT GTTAGAGCGA AACGACGTTC ATGGTTCGGC 
TTGGCTTCTC CAGTGATCCC ACTTTCGAAA GAAAAGGCAA AAGATAAGCG CCGTGGAAGT 
TCTAACGGCA CTGAGGAATT AGAGCTCGGC CCAGTATCGA ACGGGCGGGA CAATGAGACC 
GTGAAAGGAG GTAGACGAGA GGATGCGCAC GAGGAAGAGC TGAGAGAGAG AACAGGGCAC 
GAAGGCGACG ATTTGCTAAC GATTGATGGG GATCTGGAAA AGACTGTTAA AAAGAAAAAG 
CGAAAGTCTG GTGGTGCCGA CGAGCCCCTG GTACTGAGGA TGGAAGATTT GGGTCATAAA 
ACACCTGCGA GAACGATGTC TGAAAAGACG TTAACGGTAA ACGATGTGAT GAAAACACCA 
CATGCGTTAA CTACATGGAC TTTCTCTCGC ACGGCGAGCG ACCGCGATCC TTTCAGCACC 
CCTGATCTAC CGTCAAATCC AGGACCCGAC AGCTCACTTT TCAACAAACT TGAGGAGGAG 
CTTCTCCTCA ACCCAGCACC AAGATCCTCA TCCCTAAATT CGGTACCATC TCCAATTACC 
TCTTCCCATA GAGATAAAAC TCAACCCCCT TTACCTGTCG GCATGGACAG ACCGTTGCCG 
CCGTTGCCGC CCCCAACACC TGATAGTAAG GGATTGAAAG AGCCTAAAGC TCCTTCCGAA 
ATTTTTATCA CCCCCAGCAG TCCACAAGAA CCCAAGGCTA CCAGAAGAGC ACATGAAGAG 
CGGCGTAGCA GGTCTATTTC GGGACGGCCC AGAAGCCATT CACGAGGCAG AGCTTCCAGC 
GCAGAGCCCT CAAAACCTCT TGGTTTGGGA TTACCTTCAG TCATCTCGGG AAGATCTCGG 
TCTGTATCGC GCTCACGGCC ATCTTCCAGA TCACCTTCTG TATCTGTTCA AAGCCCGTCC 
CCTCCGCCCG TTCCCTTAAA ACCGCAATCA GTATCGTCGC CATTTCCATC TCGTCCGGGG 
ACTCCCAATC ATAAAATTAC TTTTGCAGAG CCCGAAGTCA CAAAACCACA AGGTTTGCAA 
AAGGAAGATG AATCTGACAA AAAGACAAGA ATCAAAAGAG CGAGAAGTTT ATCGGGATTG 
TTTGGCAAAT CACCGCCAGT GGTATTATCC GCCAAAATGG GGGATCCCGT CCAAGATGAT 
GACCAGAAGA ATGGAGAAGA GGAGACAAAC GGTGATCAAG GAGGCGGCGG AAAGCTTGGG 
GTGCTAGAAT GGTTAGGAAT GAAAAAGACT GTGAGGCGAA AAGCTTCAGA GACGAGGCTC 
AGGGAACCGG CAAGTGAGCT GGATGACGTC CAGAACGTTG CTGGTGATAC ATCTAGATTC 
CCAGAAGATC AGGCTCCTAA CAAGGGAAAT ATAGACCTAG TCAGTGAGGG TGATCAGTAC 
AACGGACAAA CCGACCTTCC ACCCCCTCCT ACCACTAACG CACCACATGG TACTCCCAAG 
AGATTGAATA GCTTATTCGC TCGCCGATCA TCTGCGAAAA ACGAGGAAGA AGCAGAGCCG 
CTGTCAATTA TGGTTGTTCC TAGGGCTATT CATAGCTCTG AATGTTCGAC ACGCCCGCTG 
ACGAACGCTT CCCAATCTTC TCTGCAAGTT CCTGCCGTCG ATCCATTCTC ACCTGATGGA 
AGCAACACGT GGACATCCAG TCCAGGCATT GAGATTGAAG AGATGGTCTT CAGCCCTGGT 
AGTAGTGCTC ATTGGGGACC TGGGATACGC CCTTGGATGG AAACAAGGCA ACATAACGTC 
ACTTCGAGGT CGTCGTTATC TTGCGCTCTG GATACGCCAG TGCAAAAGTC CGAATCTTCC 
AAAGCGCCTG CTAAAGCACA GGGAGGGCGA GTCAGGTCAT TTTCTGATGG GCCTTTGCCA 
CAACAACCAC CCGATCACTC TCCAGCTCCC TCTCAACCTG CCTCAGATAG TCCTTCTCCC 
TCTCCCTCAA CGGGCCTCAA AACACCTGTA CGACCCAGAA TGGACAGCAG GACAGGCTCC 
AGCAACTCAG CGATCGTAGG GAGAATGAAA AGTGTGTTCT CCAAGTCCTC AAACCGGAGC 
AGGAGTAACA GTCTTTTACG CCAGGATGCC AGCGATGTTG ACGAGTTTGG GGGAGTGGCC 
AGCCAACGTA TGCGACTCTC GGCGTCGGCC TCTTCTATGA GGCCTTATGA TACCGCGGGA 
GGTCACGTCA TAATGGAGGA CAGCGTCCAT CTGGAATCAC AGCAGGAAAA AAATCACACA 
GTTGGAAGAG TGTCAAGTTC GTCAATGACA CCTTCTCTTT CATCCAATGC GTCTTCTACG 
AAACATTCAG TCCTCGGAGA TCCTCTTCAT ACCCACAAAA TAGCATCACG TCGAAGCAGA 
GCTCGAGCGT CAACTGTATC TCTTGCGCCA ACTTCGTACC ATTTTGCGCC GCCTTCGCCA 
AGCACTTTTC CCATCTCTGC AACGCCACCT CGCCGCCAAA GCACAATACG TCGTCTTTCT 
AATGGACTAT TTGGATCAGC ATCCTCGTCG CCCCAACATT CTGCTCTATT TCCTCTTCCG 
CCTAGATCCA ACGGATCTAC TTCATCAGTA GCTACAGGTG CGCAGATATC TGGTCAAGGC 
TGGGAGGATG GGTCCACTGG AGTGTTAAGT CCCGGTGCTA GCCCAAGGCC CAGCTTAGGT 
AGTCTAGCCG TTAAGACCAA TGCTATGAAA CAAGCTGATG TACCGGAAGG AGAAGAATCG 
CCCAGGGAAT GGTTGGAGAG GGCTCTCAGT GCTGTTGGGA GGTGCGAAAT AGCCAATGTG 
CTGGCGTCTA GCGGCGATGC TCTTCACGTG GAAGCATTAC AGATGTTCAT GTCAACGTTC 
AACTTCACTC ATAACGCTCT GGACGTCGCT TTGAGGAAAC TGCTTATGCA AATCTCTCTT 
CCGAAAGAGA CACAGCAGAT CGATCGTGTA ATTGAAGCGT TCGCAAAACA ATATGAAAGG 
TGTGAGCCCG GTCTCTTCGG CGAAAAAGAC AACGCATATG TCCTTGCATT CTCGATGATG 
ATGCTTCACA CCGATGCCTT CAATAAGCAT AACAAGAATA AGATGTCCAA GGCAGATTAT 
GTGCGCAATT CACGCATGGA AGGCGTGCCA TCTTTCGTGC TAGAAGCTTT CTATGACAAT 
ATCACGTTTA CTCCGTTTGT CTTCATTGAG GACGACTCTG ATCTGAAGCG CACATCTGGG 
CGTACAACTC CGTTGAATTT TGGGCCGTCT ACTCCGACGT TCTCTGCCTT CTTGAACGGC 
TCTAATCCTC CCAGCAAGTC CTCCAACAAA ATTGACGTGT ACGATCTTAT CGTGCGAGAT 
ATGCTTGATC AATTGAGAGT TGATGTGGAG AAGGAAATAC CGGCTGAAAA TCCATTTTCT 
TGTCTTGGAA CTAGACCAAT CCTTGATTTC GAAGGGTTGA GCAAGGCTTT TGCTTCTGCG 
CATAGCCTTT CAATTCCTGT ACCCCAGCAG AAGACAGCAA GGAGGAACAC ACTCATAACA 
TCAGGAAAGA AGCAAGCGGC GAAACAAGAG AAGGAGTTGG ACATGGCTCT GCGAGTAACA 
AAAGTCGGGC TAATATCTCG AAAAGATGAA GCTTTTAGCG AAACGGGCAA GAAAAATCGC 
AAATGGAAAT CTTGGAGCGT TATCCTCACT CAATCACAGC TACTTTTTTT TAAAGACCCT 
ATGTGGGCTT TGACACTGCT TGAGCAAGCT AGGGCGACAT CGGAAAATGA CAAGGATGGT 
CAACTGCTCC TCCCACGAAT GGCTTCTTTC AAACCAGATG AAGTATTCCC CGTCAAGGAC 
TGCATTGCAG TTTTCGACAA GAGTTTCACT GCTTATCCAA ACACCTTTCG TTTCGTTATC 
TCTCAGCACC AACAATATCT TATGCAAGCT CGGGACGAAC TTGAGATGAA TGAGTGGATC 
ACCCTCATCA ATTACGCCTC AGCTTTTAAA AGTGCCGGTA TCAAGATGCG ACGTGGAACA 
ATGCGCAAGG ACCAAGCGGT ATTGGCGGGC GCTGCTGCTG CTGCGTCTCA TAGAAGGGAC 
ATGCGTGAGG ACCGTTATGA CTCTTCGGAC GGAGCATCTA CCCCAGGACG AGTTGCTGTA 
TTTAGGGGCG TGGACCAAAC GAGTCAAGAA AAAACACCGG CCCACCTAAA AGGTGATGGT 
GTCAGAGGCG TAGATATTGA CGGTGCCAAC GACAGTTTGC AGGAAGGTGA GCAACTGGAA 
GAGGTATTCG GCGTCGTCAA AGCGGAGCTG GCGGCTGGTA GAGGAGGCGC AAAGACTGTG 
GGCACGGCTA GGCAGCCAGA TAGGTCTTAC AGCCATGCTT CCCGTTTAGC CATTATCCAG 
AACCATCTTC GAATTCTTCG TGAGAAGGTG GTACCGATTG AATCCTCCAT TCAATCCTCT 
CTCTTGCTTG CTCGCAACGT CAACATCCTA ACTCCTTTTC AAAAGTCTAC TCGTGACCGT 
CTTGCTTCCA CTATTCCTTC ATTGGCTCGG CGCACCAGGA CTGAACGCAT CTTCCTCTCA 
AAGGTGCAAT TTTGGATTAA TGTCCTCGAG TATGAAGCGG ATCGCGCTGA ACGAGAGTGG 
AAAGAAGTGC GACACCTGGC ATTACAGGCA GCAGCAAGAA GTATGCGTGA AGATGGAGTT 
AGTGGCGTTG TTGAGGACGT CGCAAGAGAT GAGGAGGGCG GAGATCGCGG CGTACCAGTC 
CTGTCTCTCC CTAACAGCGG GGATGAAGAT GTGGAAAACG AGGCAACGCA TTTGAGCATG 
AGCCCAGGAG AACTGCCAAT CATGTTTCGA AGACCAAGCA ATGGACCCGA TAATGCTAGG 
TATCCTACTT CTCGTGATAC AAGCTCTAGT CGCGTTGCCA GCCTCCTTGA AGAAAGGGGA 
GGGGATAGAC GTCAAACTGT TTCATCCGAC TACTGGCTCA GTCCACATGA GACTGAACGG 
CTGTCGAGTG ATGTGGAAAG TGCCCATTCA AGAACCGGTA CTCCAGACAT GGGTCTTAAC 
GGATATGACG GCTCAAGGAG GGAGCGAAAA GATAGCGAAG CAGGGCGATC GAGTGGTCGA 
AACACGCCAA TGATGTTTGT CATGGAAAGC CCGATGGAAA TTGGATACGA AGAGATAAAC 
CATGAAAGGT TTTTCGAGTA TACACCTGTC AACCCCCAGA ATGGAAAAGA GACTGGGAAG 
AAGAATGAAT CGAGAGACAG TTGTGAGGAA GGAAAGAAAG AAGAGGCGGA GGATTGGCAA 
GATACGAAAG CTGCGAAAAA AGTGTCGCTA GTGCGGCCAG GCGAGATGAG TGATTGGGAG 
AGCATGCGCA AAGTCAGCCC TGGAAACAAA GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
Science (New York, N.Y.)307(5713)1321-4(2005 Feb)
Loftus BJ,Fung E,Roncaglia P,Rowley D,Amedeo P,Bruno D,Vamathevan J,Miranda M,Anderson IJ,Fraser JA,Allen JE,Bosdet IE,Brent MR,Chiu R,Doering TL,Donlin MJ,D'Souza CA,Fox DS,Grinberg V,Fu J,Fukushima M,Haas BJ,Huang JC,Janbon G,Jones SJ,Koo HL,Krzywinski MI,Kwon-Chung JK,Lengeler KB,Maiti R,Marra MA,Marra RE,Mathewson CA,Mitchell TG,Pertea M,Riggs FR,Salzberg SL,Schein JE,Shvartsbeyn A,Shin H,Shumway M,Specht CA,Suh BB,Tenney A,Utterback TR,Wickes BL,Wortman JR,Wye NH,Kronstad JW,Lodge JK,Heitman J,Davis RW,Fraser CM,Hyman RW

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CNA06050 cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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