LOC100178675 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100178675
Entrez Gene ID	100178675
Full Name	phosphatidylinositol 4-kinase alpha-like
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018812646.1	XP_018668191.1	phosphatidylinositol 4-kinase alpha-like
XM_018812646.2	XP_018668191.1	phosphatidylinositol 4-kinase alpha

Pathways from BioSystems
Gene Ontology

The following LOC100178675 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100178675 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCi73997	XM_018812646.2 Latest version!	Ciona intestinalis phosphatidylinositol 4-kinase alpha (LOC100178675), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCi73997	XM_018812646.1	Ciona intestinalis phosphatidylinositol 4-kinase alpha-like (LOC100178675), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100178675 ( XM_018812646.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018668191.1 Ciona intestinalis phosphatidylinositol 4-kinase alpha-like (LOC100178675), mRNA.

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CloneID	OCi73997
Clone ID Related Accession (Same CDS sequence)	XM_018812646.1 , XM_018812646.2
Accession Version	XM_018812646.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476892800000
Organism	Ciona intestinalis(vase tunicate)
Product	phosphatidylinositol 4-kinase alpha-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020172.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAAT TCTATTTTAA GTCTGTCGTT GGTTTGGCGA AGAAATTGGC CAAGAAAAAG 
AATGCAAGAT TGGACCAGGT GAAGTTGCTG AAAGAGTTTT GTCCCAAGAT CATAAACGGG 
TCGTACCAAC TTGACAATCG TAACTACTTT GGAATTATTG CTCTAAGCGT TTATATTGTT 
GAATCTAAAC TTGAGCACCA TGAAACATTG GTTCCTTACT TGGTTGATTT ATTGCGATAT 
CTACCTTCAG CTGCATGGAG GAAATGTAAT GTGAATAAAC AAGAGCAACT CCCACTCCCA 
GAAAGATTTG CATTTTGTTT AACAACCGTC CTTAGTGATG TCATAATCAT CTCGTTGTCG 
GGTGAATACA AAGTCAGTTA CCATGACGAT ATAACAACAG CGTTTGTTGA TACTATTACA 
TCACTATGCA AAGTGTCAAT CTCGTCAAAT ATCACTCAAG CTACTTTATA CAGCATCGTA 
CTCCCAACAT TACTTGGTTT GTTACGTGGT TTCACCCGCA CACCCAACCA CCCCATGTTT 
ACCTCTGTGT TTGGTGTGGA TGGTAGTGAA TCAGGGGAAA CACCACATAG TATGATGGGG 
AAGCCCCCAC ATAGAGATGA TTACAGAACA AAACGTTCCA GTAACTTAAT GTCGACCCCT 
GGAAAGCATC AACATAGAAA CAACATTTCA TTCATGGATG GCGACAATGG ATTCAATTGG 
TTCACTGTCA AACAAGCGGG GCAAATTATT GACGTTCTGA AAAATTTGTT ACAACCATCT 
GTGTTAAACA ATCTTGATGT GACTCTCTCA TCGATGGGGA ATGACCCACA GGGGAAATAC 
CCCCCACTAC ACTATTCATC TATATCCGAA GTGTTGGTTT TGTGCGCTGC AAAGTTCCTT 
AGAGATGTAA TTAACGTTAA TGAACTCGAT GGTGTAAAAT GCAAGGAATA CAGCAGTGTT 
GCCGAATCTA GTTTTAGCCA TGGATATTCA AAACTAAGCG TAATGTTACA GAATAAAGAG 
GATCTATTAC AACATAAACT ACAAAGGTTT CTTATGCAGT GCAGATCATG CGCTGCATGC 
CTTGATGCGA TACTTGTCGT TCACACAGAA GATAATGGGG GTTCGCAAAG TTTGTTTGAG 
AAGATGACGT CAAGGTTATT ATCATCCGAG TCAAGTCATG TCATATTGGC GCATTTGCCT 
TTGCTGCATG CGTGTTTAAA GGGCTTAGGG GTTCTAGCGA GATCTTCCCC AAGCCAAGTT 
TCCCAAATAC TTTCAGTGTT GCGCGAGTTC TTACTCAAAC CAGCACCTGC GTTGGTTAAA 
CTCAAGAAAC TTGAGAAATC GGCACCACTG GGACTTAATA ACACGGAGAC ATTAGGGAGA 
AAGGCATCCC ACACCTCTCT GATGTCATCG TTTGAAGACC AAGAGAAAGC TTCGTATGCT 
TATATTAAAA CTGTTGCCAT TCAAAGCATT TGTAGATCCT TGGAAGTTGG TTGCGAAAGT 
GACCACGATT GCGTTCCAGC TTTTCTATCC TCGCTTTCAA ACGATTTGTT CTCACCCGTC 
AAGCACGAGA GCACCAAACA CTTGGTGAAG ACACACTGCA TTGATCTAAT GTCACGTATT 
GGTGTCGCCC ATGGTTTGTC CAATCATCTC ATTATACAAC AGGTTGTCAG TACATTACAG 
CAAAGGTTGA ACTCCCCATC GTCACCTTTG GATAATCTTA TCATTGATCA ACTTGCTTCA 
ATAGCTTTGC TTGGTCCGGA CAAACTATAC CAGGAGATAT TAAACATGTT GTTGCAACTT 
TGTGTGAAAG CAAGCTCAGC TAAATATGGA GTTAAGAACG ATAACGATGC TGCTAGCTTC 
AGGCATTGTT CACTTGCGAC AATCAACGCA CTAGCCAAGA TAGCTTTGAA CTTAGACAAG 
GGAAATCCCC CAGTGGTGCA AACCCAGGGA ACCCCAATTC GTTCTCCTGC AATATTTGCT 
GACTCGCCAT TAAAAAGTCC CGAGTCACCT TCAAAAGCTC CCGAGTCACC CCCAAAGTCA 
CGACTCGAAG CACTCTTGAT GAAACTTCTT GAACTTTTCG TCCAACTTTG TCTCGAAGGA 
AAGCGCTCTG TTGACCGTGT TGGGACCACT AATGGAGGAC AGGTGTCGGG TATATTGAAG 
GCTTCTAACA GCGCTGGGAA CTTGGGTGTT CTTATCCCCA TTATAGCAGT GCTTCTCCGT 
CGTCTACCCC CCATCTACTC ACCAACACCA AGGCTTCATA AACTCTTCAG GGATTTCTGG 
CTTTACTGCG TCGTGTTTGG ATTTGCTACT GAACCAGATG GTTACACCCA AAGCTTCTGG 
CCACCTGAAT GGCACAACGC TGCTTGTGAG ATATCGGTAA AGTCACCCCT TCTAATATTC 
CCTGCTGGTG ATCCATTGAG GAATGGATTG CGTTACAACT CAGCATTGAA GAATGACACA 
GTAACACCGG GCGAACTAAA CGAACTACGA ACCTTAATCT CGAACCTTCT TGGCAACTCT 
GCTGAAATAT CGGGTCTGAT AAACTCGATG GACTTTGCTT TATGTACATA TCTACTGTCT 
GTATATAGAT TAGAGAGATT GAGAGTGCTA CACAGTGTTG AGTATTCCCC ACACGTATTA 
TTCACGTACC TAGAAGACCA AACCATTCAA AAAGATAAAT CACAAATGTG GAAGTGCATC 
AAATCTGTGG CGGATTGTGT GTTTGCTGTT TTCCTGGATA TAATTGCTTC CAAGGTTAAA 
ACCTCCAGCA CAGAAACACT ATTGGTTGAA CAAGCCCAGT TTCTCCTTAT AAAGTTCAAC 
AGCGCACAGA ATAGAATACG ACAAGTGGCT GACAGCTACT TATCCCGATT AGTGGATCGC 
TTCCCCCATG TTTTATGGAA CCAAAGATTT CTATTTACGA TGCTAAACCT TCTGCACCAG 
CTCTCGTCCA CTCTGGTTCC CCACCGTTTC TCCGAGATCC CCCCCATCCT AATCAACGGC 
ACCCACCACC ACCTCCATTT GCAAGACTCT CGTAAGATGC GAGAGAAACT CGTCGAGGAT 
TTCACCAAAC ATTGTCGTCA CATCCTCAAG GAGGCGCTAA AGTGGGCGCC TGCTACTACA 
GCGAGTCATC TGCAGAACTA CCTACTACAA GATGAAACAA CTACAACACA CAACAAGCAC 
GTTGGCGTGT CTGTGGCAGC GGAAGCTTTA TACAGCAACA AACAAACGGA AAAACCAAAT 
ATTTCGGTCG GCCACCGGAG GAATAAATAT CTTGGGGAGA TTTCTGGTAT GATTAAACTG 
GGTAAATCAT CACAATTGAA CCTTTGTGAT GTCATAATAA AAGAATACAA GAGTGCATTG 
AAAGAAAACA ATGAAACTGA AATTATGAAT TCGATATACC GAGCAACAGC ACTGCTAGTG 
GCGAATAAAG AAGAAAGCAA GCGCGAGATT CTGCATTTAA TCGCATGGTG TTGTGTAGAT 
AGACTAACAG TTCCCATCAT CGAATGCGCC ATCGAGTGTT GGCAATGGCT GTTATCCTCA 
CGTCCTAACC TCAACGTATC ATGTATGAGT GAAATAACCT CAGCATGGCA GGCAACCATT 
GACCATAGAA TGGGTGTATT CGCCCCAGAT AAGGACGAAG GGGACCCACT AGCGGTGTCT 
GAGGACCACA TACCCCACCC CGCACCTCCG TGTTGTAAAC CACATTCACT GTGGATTAAG 
TTTCTCCAGC ATAGATTAGA CGTGGTAAAA TACAGCAGTG TTGAAGAAAC CATGATCATA 
GTGGGTTTAG TGCATCGTAC GTTACCCATA CTACAACCTG GTTGTGGAAA CTATCTGTCA 
AGACACATAT CATCAGTTGG GGCGCGATGT GACCTTCTTA GCCTATCCTT CACCATGCTA 
CACAGTGTGG ATGTGAATGC GTCAAAACTG CAGAAGAATT TCTTAAGGGA AAGGATCTAT 
CTTTCAATGT TCGACTATTT CTGCTCTCCG CGTAAATTTC CGGCCCAAAA ATCCGACGAG 
CTTCGTAACG ACATCAACTC ATGTTTGCGG TTGTGGCATT TCGTCCACAC CGATCGTAAG 
CACCTCGTGC CCGATCGTGG GTCCCCTGGT ATCACCCCGA TGAGTGGGAC CCTTAGAAAG 
GTACCCAGTC ATACAGCGCT GTCGGGGTTA TCCGGGGGCA GTGGGGGTAA AGGGACAGTC 
CCAGCGGGGA TGAATCTGTT ACATGGAACG CCCTCAATGA AAAGTTTCCA AGTTGACACC 
TTGAGCCCGG ACCTCGCATC CTTGTCGAGC GCGACTATGG CCACCGGAGG CAGTGGTTGG 
ATGAATACGG TTCCCATGGC AACCAGTAGA AGGTCCACGT ATGCTCCGAG TAAGAGGAGT 
TCGAAGAAAG GGAAGGGTGA AACGATAGCC GCCCGTGAAA TGATGAAGAA ACGCACCCTT 
CTAATGTATT TAGTGACAGC TGAGTTGGAT CACCTTATAA CATGGTATAA TCCAACATCT 
GGTAGTGAGA GGGTGGTGGA AGCTGAAGCT ACTGTGGCTG CATGGAGGTC GCAGGAGATC 
ACTGACCGTC AGATGAAAGA AATGGTTCGT TTATCATGGG AGGTATCACC CATGCTGTGC 
GTGTTTCTCA CCGATAGGTT GCGGAACTCA GAATCGCTCG TGCGTGAAGT ATCAAGGTTG 
GTCAGGAGCA ACCCACAGGC TGCCCAACAT TGTCCCGAGG CTATCAAATA CCTTGTGACA 
TCACAGACGG TTGATTCTGA TTCTCCAGAG CTCCACCATG TGGTAACATG GGCGTTGGGT 
TCGCCAAGTA CCGCACTGTC CTATTTTTCA AGGTTATTCA GTCCCCACCC ACTAACAGCT 
CAATACTCGG TGAAAGTGAT GCGAAAGTTT CCACCCGAAG CGATTTTGTT CTACATTCCC 
CAACTTGTAC AATCCATAAG ATACGACACG ATGGGTTACG TACAAGATTA CCTTATTTGG 
GCATCAGGTA AATCACAATT GTTGGCACAT CAAATTATTT GGAACATGAA GACAAACATA 
TATGTGGATG AAGATGCTCA TCAGAAGGAC CCAGAGATAG GCTCCACACT TGAGCAAATT 
ATTGAAACAA TTATCTCCAA ACTATCGGGG CCCTCGCAAG ACTTTTATCG TCGTGAATTC 
AAGTTTTTCT CAGATGTTAC AGCGGTTTCT GGTACGATTA AACCTTTCCC TAAAGGCCCA 
GAGCGAACGG CTGCGTGTTT GGAAGCGCTA GCTGGTATTG AGGTGTTAGA GGGTTGCTAC 
CTCCCCAGTT CCCCCGAGTC CCTGGTGCTC GACATCGACC GTAAGAGTGG AACCCCGTTA 
CAAAGCGGGG CTAAAGCTCC TTATTTGGCA AAGTTCCGGG TCGTCGAGTG CGGGATCAGT 
GAAGTGGAAC GACTCGGTTC AATGACAACC CCATATGAAT TGCCGAGCGC CGAGAACTTA 
TCGTGGCAAG CCGTGATATT CAAAGTAGGC GACGACTGTA GGCAAGACAT GCTCGCGTTA 
CAAGTTATTC AGCTGTTTCA GAATATCTTT AAACAGGTAG GGTTGGATTT ATATTTATTC 
CCGTATCGAG TGGTGGCCAC CGCACCCGGT TGTGGCGTGA TTGAGTGCAT CCCTGATTCA 
AAGTCAAGAG ACCAACTTGG AAGGCAAACC GCTATCGGGA TGTACGAATA CTTCCGCAAC 
AAATACGGCG ACGAGAATTC TCCCAAGTTC CAAACAGCTC GTCGTAACTT TGTGAAGAGC 
ATGGCAGCTT ATAGCTTGGT GTTGTTTATA CTTCAGATTA AGGACCGTCA CAATGGCAAC 
ATAATGTTGG ACCAAGAAGG CCACCTCCTC CACATAGACT TCGGGTTCAT GTTCGAAAGT 
AGTCCCGGGG GCAACCTTGG ATGGGAACCC GATATTAAAC TTACTGAGGA GATGGTGATG 
ATAATGGGGG GGAATATGGA TGCACCCTCG TTCCAGTGGT TCATGGAGTT GTGCGTCCAA 
GCGTATCTCG CTGTACGACC GTACCGTGAA GACATTGTGT CGTTGGTGTC CCTCATGCTG 
GACACGGGAC TCCCATGTTT CCGTGGCAAC ACCATTAAGT TGTTAAGGAC GAGGTTTCAA 
CCATCGGCTT CGCCCAAGGA GGCCGCGCAG TTCATGGTTA AGGTTATCCG TGATTGCTTT 
TTAAGCAAAT GGAGCCGAAC GTACGATATG ATCCAGTATT ACCAAAACCA GATTCCTTAT 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018668191.1
CDS	22..6267
Translation	MDQFYFKSVVGLAKKLAKKKNARLDQVKLLKEFCPKIINGSYQLDNRNYFGIIALSVYIVESKLEHHETLVPYLVDLLRYLPSAAWRKCNVNKQEQLPLPERFAFCLTTVLSDVIIISLSGEYKVSYHDDITTAFVDTITSLCKVSISSNITQATLYSIVLPTLLGLLRGFTRTPNHPMFTSVFGVDGSESGETPHSMMGKPPHRDDYRTKRSSNLMSTPGKHQHRNNISFMDGDNGFNWFTVKQAGQIIDVLKNLLQPSVLNNLDVTLSSMGNDPQGKYPPLHYSSISEVLVLCAAKFLRDVINVNELDGVKCKEYSSVAESSFSHGYSKLSVMLQNKEDLLQHKLQRFLMQCRSCAACLDAILVVHTEDNGGSQSLFEKMTSRLLSSESSHVILAHLPLLHACLKGLGVLARSSPSQVSQILSVLREFLLKPAPALVKLKKLEKSAPLGLNNTETLGRKASHTSLMSSFEDQEKASYAYIKTVAIQSICRSLEVGCESDHDCVPAFLSSLSNDLFSPVKHESTKHLVKTHCIDLMSRIGVAHGLSNHLIIQQVVSTLQQRLNSPSSPLDNLIIDQLASIALLGPDKLYQEILNMLLQLCVKASSAKYGVKNDNDAASFRHCSLATINALAKIALNLDKGNPPVVQTQGTPIRSPAIFADSPLKSPESPSKAPESPPKSRLEALLMKLLELFVQLCLEGKRSVDRVGTTNGGQVSGILKASNSAGNLGVLIPIIAVLLRRLPPIYSPTPRLHKLFRDFWLYCVVFGFATEPDGYTQSFWPPEWHNAACEISVKSPLLIFPAGDPLRNGLRYNSALKNDTVTPGELNELRTLISNLLGNSAEISGLINSMDFALCTYLLSVYRLERLRVLHSVEYSPHVLFTYLEDQTIQKDKSQMWKCIKSVADCVFAVFLDIIASKVKTSSTETLLVEQAQFLLIKFNSAQNRIRQVADSYLSRLVDRFPHVLWNQRFLFTMLNLLHQLSSTLVPHRFSEIPPILINGTHHHLHLQDSRKMREKLVEDFTKHCRHILKEALKWAPATTASHLQNYLLQDETTTTHNKHVGVSVAAEALYSNKQTEKPNISVGHRRNKYLGEISGMIKLGKSSQLNLCDVIIKEYKSALKENNETEIMNSIYRATALLVANKEESKREILHLIAWCCVDRLTVPIIECAIECWQWLLSSRPNLNVSCMSEITSAWQATIDHRMGVFAPDKDEGDPLAVSEDHIPHPAPPCCKPHSLWIKFLQHRLDVVKYSSVEETMIIVGLVHRTLPILQPGCGNYLSRHISSVGARCDLLSLSFTMLHSVDVNASKLQKNFLRERIYLSMFDYFCSPRKFPAQKSDELRNDINSCLRLWHFVHTDRKHLVPDRGSPGITPMSGTLRKVPSHTALSGLSGGSGGKGTVPAGMNLLHGTPSMKSFQVDTLSPDLASLSSATMATGGSGWMNTVPMATSRRSTYAPSKRSSKKGKGETIAAREMMKKRTLLMYLVTAELDHLITWYNPTSGSERVVEAEATVAAWRSQEITDRQMKEMVRLSWEVSPMLCVFLTDRLRNSESLVREVSRLVRSNPQAAQHCPEAIKYLVTSQTVDSDSPELHHVVTWALGSPSTALSYFSRLFSPHPLTAQYSVKVMRKFPPEAILFYIPQLVQSIRYDTMGYVQDYLIWASGKSQLLAHQIIWNMKTNIYVDEDAHQKDPEIGSTLEQIIETIISKLSGPSQDFYRREFKFFSDVTAVSGTIKPFPKGPERTAACLEALAGIEVLEGCYLPSSPESLVLDIDRKSGTPLQSGAKAPYLAKFRVVECGISEVERLGSMTTPYELPSAENLSWQAVIFKVGDDCRQDMLALQVIQLFQNIFKQVGLDLYLFPYRVVATAPGCGVIECIPDSKSRDQLGRQTAIGMYEYFRNKYGDENSPKFQTARRNFVKSMAAYSLVLFILQIKDRHNGNIMLDQEGHLLHIDFGFMFESSPGGNLGWEPDIKLTEEMVMIMGGNMDAPSFQWFMELCVQAYLAVRPYREDIVSLVSLMLDTGLPCFRGNTIKLLRTRFQPSASPKEAAQFMVKVIRDCFLSKWSRTYDMIQYYQNQIPYY

Target ORF information:

RefSeq Version	XM_018812646.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis phosphatidylinositol 4-kinase alpha-like (LOC100178675), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018812646.1

ORF Insert Sequence:

ATGGATCAAT TCTATTTTAA GTCTGTCGTT GGTTTGGCGA AGAAATTGGC CAAGAAAAAG 
AATGCAAGAT TGGACCAGGT GAAGTTGCTG AAAGAGTTTT GTCCCAAGAT CATAAACGGG 
TCGTACCAAC TTGACAATCG TAACTACTTT GGAATTATTG CTCTAAGCGT TTATATTGTT 
GAATCTAAAC TTGAGCACCA TGAAACATTG GTTCCTTACT TGGTTGATTT ATTGCGATAT 
CTACCTTCAG CTGCATGGAG GAAATGTAAT GTGAATAAAC AAGAGCAACT CCCACTCCCA 
GAAAGATTTG CATTTTGTTT AACAACCGTC CTTAGTGATG TCATAATCAT CTCGTTGTCG 
GGTGAATACA AAGTCAGTTA CCATGACGAT ATAACAACAG CGTTTGTTGA TACTATTACA 
TCACTATGCA AAGTGTCAAT CTCGTCAAAT ATCACTCAAG CTACTTTATA CAGCATCGTA 
CTCCCAACAT TACTTGGTTT GTTACGTGGT TTCACCCGCA CACCCAACCA CCCCATGTTT 
ACCTCTGTGT TTGGTGTGGA TGGTAGTGAA TCAGGGGAAA CACCACATAG TATGATGGGG 
AAGCCCCCAC ATAGAGATGA TTACAGAACA AAACGTTCCA GTAACTTAAT GTCGACCCCT 
GGAAAGCATC AACATAGAAA CAACATTTCA TTCATGGATG GCGACAATGG ATTCAATTGG 
TTCACTGTCA AACAAGCGGG GCAAATTATT GACGTTCTGA AAAATTTGTT ACAACCATCT 
GTGTTAAACA ATCTTGATGT GACTCTCTCA TCGATGGGGA ATGACCCACA GGGGAAATAC 
CCCCCACTAC ACTATTCATC TATATCCGAA GTGTTGGTTT TGTGCGCTGC AAAGTTCCTT 
AGAGATGTAA TTAACGTTAA TGAACTCGAT GGTGTAAAAT GCAAGGAATA CAGCAGTGTT 
GCCGAATCTA GTTTTAGCCA TGGATATTCA AAACTAAGCG TAATGTTACA GAATAAAGAG 
GATCTATTAC AACATAAACT ACAAAGGTTT CTTATGCAGT GCAGATCATG CGCTGCATGC 
CTTGATGCGA TACTTGTCGT TCACACAGAA GATAATGGGG GTTCGCAAAG TTTGTTTGAG 
AAGATGACGT CAAGGTTATT ATCATCCGAG TCAAGTCATG TCATATTGGC GCATTTGCCT 
TTGCTGCATG CGTGTTTAAA GGGCTTAGGG GTTCTAGCGA GATCTTCCCC AAGCCAAGTT 
TCCCAAATAC TTTCAGTGTT GCGCGAGTTC TTACTCAAAC CAGCACCTGC GTTGGTTAAA 
CTCAAGAAAC TTGAGAAATC GGCACCACTG GGACTTAATA ACACGGAGAC ATTAGGGAGA 
AAGGCATCCC ACACCTCTCT GATGTCATCG TTTGAAGACC AAGAGAAAGC TTCGTATGCT 
TATATTAAAA CTGTTGCCAT TCAAAGCATT TGTAGATCCT TGGAAGTTGG TTGCGAAAGT 
GACCACGATT GCGTTCCAGC TTTTCTATCC TCGCTTTCAA ACGATTTGTT CTCACCCGTC 
AAGCACGAGA GCACCAAACA CTTGGTGAAG ACACACTGCA TTGATCTAAT GTCACGTATT 
GGTGTCGCCC ATGGTTTGTC CAATCATCTC ATTATACAAC AGGTTGTCAG TACATTACAG 
CAAAGGTTGA ACTCCCCATC GTCACCTTTG GATAATCTTA TCATTGATCA ACTTGCTTCA 
ATAGCTTTGC TTGGTCCGGA CAAACTATAC CAGGAGATAT TAAACATGTT GTTGCAACTT 
TGTGTGAAAG CAAGCTCAGC TAAATATGGA GTTAAGAACG ATAACGATGC TGCTAGCTTC 
AGGCATTGTT CACTTGCGAC AATCAACGCA CTAGCCAAGA TAGCTTTGAA CTTAGACAAG 
GGAAATCCCC CAGTGGTGCA AACCCAGGGA ACCCCAATTC GTTCTCCTGC AATATTTGCT 
GACTCGCCAT TAAAAAGTCC CGAGTCACCT TCAAAAGCTC CCGAGTCACC CCCAAAGTCA 
CGACTCGAAG CACTCTTGAT GAAACTTCTT GAACTTTTCG TCCAACTTTG TCTCGAAGGA 
AAGCGCTCTG TTGACCGTGT TGGGACCACT AATGGAGGAC AGGTGTCGGG TATATTGAAG 
GCTTCTAACA GCGCTGGGAA CTTGGGTGTT CTTATCCCCA TTATAGCAGT GCTTCTCCGT 
CGTCTACCCC CCATCTACTC ACCAACACCA AGGCTTCATA AACTCTTCAG GGATTTCTGG 
CTTTACTGCG TCGTGTTTGG ATTTGCTACT GAACCAGATG GTTACACCCA AAGCTTCTGG 
CCACCTGAAT GGCACAACGC TGCTTGTGAG ATATCGGTAA AGTCACCCCT TCTAATATTC 
CCTGCTGGTG ATCCATTGAG GAATGGATTG CGTTACAACT CAGCATTGAA GAATGACACA 
GTAACACCGG GCGAACTAAA CGAACTACGA ACCTTAATCT CGAACCTTCT TGGCAACTCT 
GCTGAAATAT CGGGTCTGAT AAACTCGATG GACTTTGCTT TATGTACATA TCTACTGTCT 
GTATATAGAT TAGAGAGATT GAGAGTGCTA CACAGTGTTG AGTATTCCCC ACACGTATTA 
TTCACGTACC TAGAAGACCA AACCATTCAA AAAGATAAAT CACAAATGTG GAAGTGCATC 
AAATCTGTGG CGGATTGTGT GTTTGCTGTT TTCCTGGATA TAATTGCTTC CAAGGTTAAA 
ACCTCCAGCA CAGAAACACT ATTGGTTGAA CAAGCCCAGT TTCTCCTTAT AAAGTTCAAC 
AGCGCACAGA ATAGAATACG ACAAGTGGCT GACAGCTACT TATCCCGATT AGTGGATCGC 
TTCCCCCATG TTTTATGGAA CCAAAGATTT CTATTTACGA TGCTAAACCT TCTGCACCAG 
CTCTCGTCCA CTCTGGTTCC CCACCGTTTC TCCGAGATCC CCCCCATCCT AATCAACGGC 
ACCCACCACC ACCTCCATTT GCAAGACTCT CGTAAGATGC GAGAGAAACT CGTCGAGGAT 
TTCACCAAAC ATTGTCGTCA CATCCTCAAG GAGGCGCTAA AGTGGGCGCC TGCTACTACA 
GCGAGTCATC TGCAGAACTA CCTACTACAA GATGAAACAA CTACAACACA CAACAAGCAC 
GTTGGCGTGT CTGTGGCAGC GGAAGCTTTA TACAGCAACA AACAAACGGA AAAACCAAAT 
ATTTCGGTCG GCCACCGGAG GAATAAATAT CTTGGGGAGA TTTCTGGTAT GATTAAACTG 
GGTAAATCAT CACAATTGAA CCTTTGTGAT GTCATAATAA AAGAATACAA GAGTGCATTG 
AAAGAAAACA ATGAAACTGA AATTATGAAT TCGATATACC GAGCAACAGC ACTGCTAGTG 
GCGAATAAAG AAGAAAGCAA GCGCGAGATT CTGCATTTAA TCGCATGGTG TTGTGTAGAT 
AGACTAACAG TTCCCATCAT CGAATGCGCC ATCGAGTGTT GGCAATGGCT GTTATCCTCA 
CGTCCTAACC TCAACGTATC ATGTATGAGT GAAATAACCT CAGCATGGCA GGCAACCATT 
GACCATAGAA TGGGTGTATT CGCCCCAGAT AAGGACGAAG GGGACCCACT AGCGGTGTCT 
GAGGACCACA TACCCCACCC CGCACCTCCG TGTTGTAAAC CACATTCACT GTGGATTAAG 
TTTCTCCAGC ATAGATTAGA CGTGGTAAAA TACAGCAGTG TTGAAGAAAC CATGATCATA 
GTGGGTTTAG TGCATCGTAC GTTACCCATA CTACAACCTG GTTGTGGAAA CTATCTGTCA 
AGACACATAT CATCAGTTGG GGCGCGATGT GACCTTCTTA GCCTATCCTT CACCATGCTA 
CACAGTGTGG ATGTGAATGC GTCAAAACTG CAGAAGAATT TCTTAAGGGA AAGGATCTAT 
CTTTCAATGT TCGACTATTT CTGCTCTCCG CGTAAATTTC CGGCCCAAAA ATCCGACGAG 
CTTCGTAACG ACATCAACTC ATGTTTGCGG TTGTGGCATT TCGTCCACAC CGATCGTAAG 
CACCTCGTGC CCGATCGTGG GTCCCCTGGT ATCACCCCGA TGAGTGGGAC CCTTAGAAAG 
GTACCCAGTC ATACAGCGCT GTCGGGGTTA TCCGGGGGCA GTGGGGGTAA AGGGACAGTC 
CCAGCGGGGA TGAATCTGTT ACATGGAACG CCCTCAATGA AAAGTTTCCA AGTTGACACC 
TTGAGCCCGG ACCTCGCATC CTTGTCGAGC GCGACTATGG CCACCGGAGG CAGTGGTTGG 
ATGAATACGG TTCCCATGGC AACCAGTAGA AGGTCCACGT ATGCTCCGAG TAAGAGGAGT 
TCGAAGAAAG GGAAGGGTGA AACGATAGCC GCCCGTGAAA TGATGAAGAA ACGCACCCTT 
CTAATGTATT TAGTGACAGC TGAGTTGGAT CACCTTATAA CATGGTATAA TCCAACATCT 
GGTAGTGAGA GGGTGGTGGA AGCTGAAGCT ACTGTGGCTG CATGGAGGTC GCAGGAGATC 
ACTGACCGTC AGATGAAAGA AATGGTTCGT TTATCATGGG AGGTATCACC CATGCTGTGC 
GTGTTTCTCA CCGATAGGTT GCGGAACTCA GAATCGCTCG TGCGTGAAGT ATCAAGGTTG 
GTCAGGAGCA ACCCACAGGC TGCCCAACAT TGTCCCGAGG CTATCAAATA CCTTGTGACA 
TCACAGACGG TTGATTCTGA TTCTCCAGAG CTCCACCATG TGGTAACATG GGCGTTGGGT 
TCGCCAAGTA CCGCACTGTC CTATTTTTCA AGGTTATTCA GTCCCCACCC ACTAACAGCT 
CAATACTCGG TGAAAGTGAT GCGAAAGTTT CCACCCGAAG CGATTTTGTT CTACATTCCC 
CAACTTGTAC AATCCATAAG ATACGACACG ATGGGTTACG TACAAGATTA CCTTATTTGG 
GCATCAGGTA AATCACAATT GTTGGCACAT CAAATTATTT GGAACATGAA GACAAACATA 
TATGTGGATG AAGATGCTCA TCAGAAGGAC CCAGAGATAG GCTCCACACT TGAGCAAATT 
ATTGAAACAA TTATCTCCAA ACTATCGGGG CCCTCGCAAG ACTTTTATCG TCGTGAATTC 
AAGTTTTTCT CAGATGTTAC AGCGGTTTCT GGTACGATTA AACCTTTCCC TAAAGGCCCA 
GAGCGAACGG CTGCGTGTTT GGAAGCGCTA GCTGGTATTG AGGTGTTAGA GGGTTGCTAC 
CTCCCCAGTT CCCCCGAGTC CCTGGTGCTC GACATCGACC GTAAGAGTGG AACCCCGTTA 
CAAAGCGGGG CTAAAGCTCC TTATTTGGCA AAGTTCCGGG TCGTCGAGTG CGGGATCAGT 
GAAGTGGAAC GACTCGGTTC AATGACAACC CCATATGAAT TGCCGAGCGC CGAGAACTTA 
TCGTGGCAAG CCGTGATATT CAAAGTAGGC GACGACTGTA GGCAAGACAT GCTCGCGTTA 
CAAGTTATTC AGCTGTTTCA GAATATCTTT AAACAGGTAG GGTTGGATTT ATATTTATTC 
CCGTATCGAG TGGTGGCCAC CGCACCCGGT TGTGGCGTGA TTGAGTGCAT CCCTGATTCA 
AAGTCAAGAG ACCAACTTGG AAGGCAAACC GCTATCGGGA TGTACGAATA CTTCCGCAAC 
AAATACGGCG ACGAGAATTC TCCCAAGTTC CAAACAGCTC GTCGTAACTT TGTGAAGAGC 
ATGGCAGCTT ATAGCTTGGT GTTGTTTATA CTTCAGATTA AGGACCGTCA CAATGGCAAC 
ATAATGTTGG ACCAAGAAGG CCACCTCCTC CACATAGACT TCGGGTTCAT GTTCGAAAGT 
AGTCCCGGGG GCAACCTTGG ATGGGAACCC GATATTAAAC TTACTGAGGA GATGGTGATG 
ATAATGGGGG GGAATATGGA TGCACCCTCG TTCCAGTGGT TCATGGAGTT GTGCGTCCAA 
GCGTATCTCG CTGTACGACC GTACCGTGAA GACATTGTGT CGTTGGTGTC CCTCATGCTG 
GACACGGGAC TCCCATGTTT CCGTGGCAAC ACCATTAAGT TGTTAAGGAC GAGGTTTCAA 
CCATCGGCTT CGCCCAAGGA GGCCGCGCAG TTCATGGTTA AGGTTATCCG TGATTGCTTT 
TTAAGCAAAT GGAGCCGAAC GTACGATATG ATCCAGTATT ACCAAAACCA GATTCCTTAT 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100178675 ( XM_018812646.2 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018668191.1 Ciona intestinalis phosphatidylinositol 4-kinase alpha (LOC100178675), mRNA.

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CloneID	OCi73997
Clone ID Related Accession (Same CDS sequence)	XM_018812646.1 , XM_018812646.2
Accession Version	XM_018812646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1540051200000
Organism	Ciona intestinalis(vase tunicate)
Product	phosphatidylinositol 4-kinase alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020172.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 22, 2018 this sequence version replaced XM_018812646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAAT TCTATTTTAA GTCTGTCGTT GGTTTGGCGA AGAAATTGGC CAAGAAAAAG 
AATGCAAGAT TGGACCAGGT GAAGTTGCTG AAAGAGTTTT GTCCCAAGAT CATAAACGGG 
TCGTACCAAC TTGACAATCG TAACTACTTT GGAATTATTG CTCTAAGCGT TTATATTGTT 
GAATCTAAAC TTGAGCACCA TGAAACATTG GTTCCTTACT TGGTTGATTT ATTGCGATAT 
CTACCTTCAG CTGCATGGAG GAAATGTAAT GTGAATAAAC AAGAGCAACT CCCACTCCCA 
GAAAGATTTG CATTTTGTTT AACAACCGTC CTTAGTGATG TCATAATCAT CTCGTTGTCG 
GGTGAATACA AAGTCAGTTA CCATGACGAT ATAACAACAG CGTTTGTTGA TACTATTACA 
TCACTATGCA AAGTGTCAAT CTCGTCAAAT ATCACTCAAG CTACTTTATA CAGCATCGTA 
CTCCCAACAT TACTTGGTTT GTTACGTGGT TTCACCCGCA CACCCAACCA CCCCATGTTT 
ACCTCTGTGT TTGGTGTGGA TGGTAGTGAA TCAGGGGAAA CACCACATAG TATGATGGGG 
AAGCCCCCAC ATAGAGATGA TTACAGAACA AAACGTTCCA GTAACTTAAT GTCGACCCCT 
GGAAAGCATC AACATAGAAA CAACATTTCA TTCATGGATG GCGACAATGG ATTCAATTGG 
TTCACTGTCA AACAAGCGGG GCAAATTATT GACGTTCTGA AAAATTTGTT ACAACCATCT 
GTGTTAAACA ATCTTGATGT GACTCTCTCA TCGATGGGGA ATGACCCACA GGGGAAATAC 
CCCCCACTAC ACTATTCATC TATATCCGAA GTGTTGGTTT TGTGCGCTGC AAAGTTCCTT 
AGAGATGTAA TTAACGTTAA TGAACTCGAT GGTGTAAAAT GCAAGGAATA CAGCAGTGTT 
GCCGAATCTA GTTTTAGCCA TGGATATTCA AAACTAAGCG TAATGTTACA GAATAAAGAG 
GATCTATTAC AACATAAACT ACAAAGGTTT CTTATGCAGT GCAGATCATG CGCTGCATGC 
CTTGATGCGA TACTTGTCGT TCACACAGAA GATAATGGGG GTTCGCAAAG TTTGTTTGAG 
AAGATGACGT CAAGGTTATT ATCATCCGAG TCAAGTCATG TCATATTGGC GCATTTGCCT 
TTGCTGCATG CGTGTTTAAA GGGCTTAGGG GTTCTAGCGA GATCTTCCCC AAGCCAAGTT 
TCCCAAATAC TTTCAGTGTT GCGCGAGTTC TTACTCAAAC CAGCACCTGC GTTGGTTAAA 
CTCAAGAAAC TTGAGAAATC GGCACCACTG GGACTTAATA ACACGGAGAC ATTAGGGAGA 
AAGGCATCCC ACACCTCTCT GATGTCATCG TTTGAAGACC AAGAGAAAGC TTCGTATGCT 
TATATTAAAA CTGTTGCCAT TCAAAGCATT TGTAGATCCT TGGAAGTTGG TTGCGAAAGT 
GACCACGATT GCGTTCCAGC TTTTCTATCC TCGCTTTCAA ACGATTTGTT CTCACCCGTC 
AAGCACGAGA GCACCAAACA CTTGGTGAAG ACACACTGCA TTGATCTAAT GTCACGTATT 
GGTGTCGCCC ATGGTTTGTC CAATCATCTC ATTATACAAC AGGTTGTCAG TACATTACAG 
CAAAGGTTGA ACTCCCCATC GTCACCTTTG GATAATCTTA TCATTGATCA ACTTGCTTCA 
ATAGCTTTGC TTGGTCCGGA CAAACTATAC CAGGAGATAT TAAACATGTT GTTGCAACTT 
TGTGTGAAAG CAAGCTCAGC TAAATATGGA GTTAAGAACG ATAACGATGC TGCTAGCTTC 
AGGCATTGTT CACTTGCGAC AATCAACGCA CTAGCCAAGA TAGCTTTGAA CTTAGACAAG 
GGAAATCCCC CAGTGGTGCA AACCCAGGGA ACCCCAATTC GTTCTCCTGC AATATTTGCT 
GACTCGCCAT TAAAAAGTCC CGAGTCACCT TCAAAAGCTC CCGAGTCACC CCCAAAGTCA 
CGACTCGAAG CACTCTTGAT GAAACTTCTT GAACTTTTCG TCCAACTTTG TCTCGAAGGA 
AAGCGCTCTG TTGACCGTGT TGGGACCACT AATGGAGGAC AGGTGTCGGG TATATTGAAG 
GCTTCTAACA GCGCTGGGAA CTTGGGTGTT CTTATCCCCA TTATAGCAGT GCTTCTCCGT 
CGTCTACCCC CCATCTACTC ACCAACACCA AGGCTTCATA AACTCTTCAG GGATTTCTGG 
CTTTACTGCG TCGTGTTTGG ATTTGCTACT GAACCAGATG GTTACACCCA AAGCTTCTGG 
CCACCTGAAT GGCACAACGC TGCTTGTGAG ATATCGGTAA AGTCACCCCT TCTAATATTC 
CCTGCTGGTG ATCCATTGAG GAATGGATTG CGTTACAACT CAGCATTGAA GAATGACACA 
GTAACACCGG GCGAACTAAA CGAACTACGA ACCTTAATCT CGAACCTTCT TGGCAACTCT 
GCTGAAATAT CGGGTCTGAT AAACTCGATG GACTTTGCTT TATGTACATA TCTACTGTCT 
GTATATAGAT TAGAGAGATT GAGAGTGCTA CACAGTGTTG AGTATTCCCC ACACGTATTA 
TTCACGTACC TAGAAGACCA AACCATTCAA AAAGATAAAT CACAAATGTG GAAGTGCATC 
AAATCTGTGG CGGATTGTGT GTTTGCTGTT TTCCTGGATA TAATTGCTTC CAAGGTTAAA 
ACCTCCAGCA CAGAAACACT ATTGGTTGAA CAAGCCCAGT TTCTCCTTAT AAAGTTCAAC 
AGCGCACAGA ATAGAATACG ACAAGTGGCT GACAGCTACT TATCCCGATT AGTGGATCGC 
TTCCCCCATG TTTTATGGAA CCAAAGATTT CTATTTACGA TGCTAAACCT TCTGCACCAG 
CTCTCGTCCA CTCTGGTTCC CCACCGTTTC TCCGAGATCC CCCCCATCCT AATCAACGGC 
ACCCACCACC ACCTCCATTT GCAAGACTCT CGTAAGATGC GAGAGAAACT CGTCGAGGAT 
TTCACCAAAC ATTGTCGTCA CATCCTCAAG GAGGCGCTAA AGTGGGCGCC TGCTACTACA 
GCGAGTCATC TGCAGAACTA CCTACTACAA GATGAAACAA CTACAACACA CAACAAGCAC 
GTTGGCGTGT CTGTGGCAGC GGAAGCTTTA TACAGCAACA AACAAACGGA AAAACCAAAT 
ATTTCGGTCG GCCACCGGAG GAATAAATAT CTTGGGGAGA TTTCTGGTAT GATTAAACTG 
GGTAAATCAT CACAATTGAA CCTTTGTGAT GTCATAATAA AAGAATACAA GAGTGCATTG 
AAAGAAAACA ATGAAACTGA AATTATGAAT TCGATATACC GAGCAACAGC ACTGCTAGTG 
GCGAATAAAG AAGAAAGCAA GCGCGAGATT CTGCATTTAA TCGCATGGTG TTGTGTAGAT 
AGACTAACAG TTCCCATCAT CGAATGCGCC ATCGAGTGTT GGCAATGGCT GTTATCCTCA 
CGTCCTAACC TCAACGTATC ATGTATGAGT GAAATAACCT CAGCATGGCA GGCAACCATT 
GACCATAGAA TGGGTGTATT CGCCCCAGAT AAGGACGAAG GGGACCCACT AGCGGTGTCT 
GAGGACCACA TACCCCACCC CGCACCTCCG TGTTGTAAAC CACATTCACT GTGGATTAAG 
TTTCTCCAGC ATAGATTAGA CGTGGTAAAA TACAGCAGTG TTGAAGAAAC CATGATCATA 
GTGGGTTTAG TGCATCGTAC GTTACCCATA CTACAACCTG GTTGTGGAAA CTATCTGTCA 
AGACACATAT CATCAGTTGG GGCGCGATGT GACCTTCTTA GCCTATCCTT CACCATGCTA 
CACAGTGTGG ATGTGAATGC GTCAAAACTG CAGAAGAATT TCTTAAGGGA AAGGATCTAT 
CTTTCAATGT TCGACTATTT CTGCTCTCCG CGTAAATTTC CGGCCCAAAA ATCCGACGAG 
CTTCGTAACG ACATCAACTC ATGTTTGCGG TTGTGGCATT TCGTCCACAC CGATCGTAAG 
CACCTCGTGC CCGATCGTGG GTCCCCTGGT ATCACCCCGA TGAGTGGGAC CCTTAGAAAG 
GTACCCAGTC ATACAGCGCT GTCGGGGTTA TCCGGGGGCA GTGGGGGTAA AGGGACAGTC 
CCAGCGGGGA TGAATCTGTT ACATGGAACG CCCTCAATGA AAAGTTTCCA AGTTGACACC 
TTGAGCCCGG ACCTCGCATC CTTGTCGAGC GCGACTATGG CCACCGGAGG CAGTGGTTGG 
ATGAATACGG TTCCCATGGC AACCAGTAGA AGGTCCACGT ATGCTCCGAG TAAGAGGAGT 
TCGAAGAAAG GGAAGGGTGA AACGATAGCC GCCCGTGAAA TGATGAAGAA ACGCACCCTT 
CTAATGTATT TAGTGACAGC TGAGTTGGAT CACCTTATAA CATGGTATAA TCCAACATCT 
GGTAGTGAGA GGGTGGTGGA AGCTGAAGCT ACTGTGGCTG CATGGAGGTC GCAGGAGATC 
ACTGACCGTC AGATGAAAGA AATGGTTCGT TTATCATGGG AGGTATCACC CATGCTGTGC 
GTGTTTCTCA CCGATAGGTT GCGGAACTCA GAATCGCTCG TGCGTGAAGT ATCAAGGTTG 
GTCAGGAGCA ACCCACAGGC TGCCCAACAT TGTCCCGAGG CTATCAAATA CCTTGTGACA 
TCACAGACGG TTGATTCTGA TTCTCCAGAG CTCCACCATG TGGTAACATG GGCGTTGGGT 
TCGCCAAGTA CCGCACTGTC CTATTTTTCA AGGTTATTCA GTCCCCACCC ACTAACAGCT 
CAATACTCGG TGAAAGTGAT GCGAAAGTTT CCACCCGAAG CGATTTTGTT CTACATTCCC 
CAACTTGTAC AATCCATAAG ATACGACACG ATGGGTTACG TACAAGATTA CCTTATTTGG 
GCATCAGGTA AATCACAATT GTTGGCACAT CAAATTATTT GGAACATGAA GACAAACATA 
TATGTGGATG AAGATGCTCA TCAGAAGGAC CCAGAGATAG GCTCCACACT TGAGCAAATT 
ATTGAAACAA TTATCTCCAA ACTATCGGGG CCCTCGCAAG ACTTTTATCG TCGTGAATTC 
AAGTTTTTCT CAGATGTTAC AGCGGTTTCT GGTACGATTA AACCTTTCCC TAAAGGCCCA 
GAGCGAACGG CTGCGTGTTT GGAAGCGCTA GCTGGTATTG AGGTGTTAGA GGGTTGCTAC 
CTCCCCAGTT CCCCCGAGTC CCTGGTGCTC GACATCGACC GTAAGAGTGG AACCCCGTTA 
CAAAGCGGGG CTAAAGCTCC TTATTTGGCA AAGTTCCGGG TCGTCGAGTG CGGGATCAGT 
GAAGTGGAAC GACTCGGTTC AATGACAACC CCATATGAAT TGCCGAGCGC CGAGAACTTA 
TCGTGGCAAG CCGTGATATT CAAAGTAGGC GACGACTGTA GGCAAGACAT GCTCGCGTTA 
CAAGTTATTC AGCTGTTTCA GAATATCTTT AAACAGGTAG GGTTGGATTT ATATTTATTC 
CCGTATCGAG TGGTGGCCAC CGCACCCGGT TGTGGCGTGA TTGAGTGCAT CCCTGATTCA 
AAGTCAAGAG ACCAACTTGG AAGGCAAACC GCTATCGGGA TGTACGAATA CTTCCGCAAC 
AAATACGGCG ACGAGAATTC TCCCAAGTTC CAAACAGCTC GTCGTAACTT TGTGAAGAGC 
ATGGCAGCTT ATAGCTTGGT GTTGTTTATA CTTCAGATTA AGGACCGTCA CAATGGCAAC 
ATAATGTTGG ACCAAGAAGG CCACCTCCTC CACATAGACT TCGGGTTCAT GTTCGAAAGT 
AGTCCCGGGG GCAACCTTGG ATGGGAACCC GATATTAAAC TTACTGAGGA GATGGTGATG 
ATAATGGGGG GGAATATGGA TGCACCCTCG TTCCAGTGGT TCATGGAGTT GTGCGTCCAA 
GCGTATCTCG CTGTACGACC GTACCGTGAA GACATTGTGT CGTTGGTGTC CCTCATGCTG 
GACACGGGAC TCCCATGTTT CCGTGGCAAC ACCATTAAGT TGTTAAGGAC GAGGTTTCAA 
CCATCGGCTT CGCCCAAGGA GGCCGCGCAG TTCATGGTTA AGGTTATCCG TGATTGCTTT 
TTAAGCAAAT GGAGCCGAAC GTACGATATG ATCCAGTATT ACCAAAACCA GATTCCTTAT 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018668191.1
CDS	85..6330
Translation	MDQFYFKSVVGLAKKLAKKKNARLDQVKLLKEFCPKIINGSYQLDNRNYFGIIALSVYIVESKLEHHETLVPYLVDLLRYLPSAAWRKCNVNKQEQLPLPERFAFCLTTVLSDVIIISLSGEYKVSYHDDITTAFVDTITSLCKVSISSNITQATLYSIVLPTLLGLLRGFTRTPNHPMFTSVFGVDGSESGETPHSMMGKPPHRDDYRTKRSSNLMSTPGKHQHRNNISFMDGDNGFNWFTVKQAGQIIDVLKNLLQPSVLNNLDVTLSSMGNDPQGKYPPLHYSSISEVLVLCAAKFLRDVINVNELDGVKCKEYSSVAESSFSHGYSKLSVMLQNKEDLLQHKLQRFLMQCRSCAACLDAILVVHTEDNGGSQSLFEKMTSRLLSSESSHVILAHLPLLHACLKGLGVLARSSPSQVSQILSVLREFLLKPAPALVKLKKLEKSAPLGLNNTETLGRKASHTSLMSSFEDQEKASYAYIKTVAIQSICRSLEVGCESDHDCVPAFLSSLSNDLFSPVKHESTKHLVKTHCIDLMSRIGVAHGLSNHLIIQQVVSTLQQRLNSPSSPLDNLIIDQLASIALLGPDKLYQEILNMLLQLCVKASSAKYGVKNDNDAASFRHCSLATINALAKIALNLDKGNPPVVQTQGTPIRSPAIFADSPLKSPESPSKAPESPPKSRLEALLMKLLELFVQLCLEGKRSVDRVGTTNGGQVSGILKASNSAGNLGVLIPIIAVLLRRLPPIYSPTPRLHKLFRDFWLYCVVFGFATEPDGYTQSFWPPEWHNAACEISVKSPLLIFPAGDPLRNGLRYNSALKNDTVTPGELNELRTLISNLLGNSAEISGLINSMDFALCTYLLSVYRLERLRVLHSVEYSPHVLFTYLEDQTIQKDKSQMWKCIKSVADCVFAVFLDIIASKVKTSSTETLLVEQAQFLLIKFNSAQNRIRQVADSYLSRLVDRFPHVLWNQRFLFTMLNLLHQLSSTLVPHRFSEIPPILINGTHHHLHLQDSRKMREKLVEDFTKHCRHILKEALKWAPATTASHLQNYLLQDETTTTHNKHVGVSVAAEALYSNKQTEKPNISVGHRRNKYLGEISGMIKLGKSSQLNLCDVIIKEYKSALKENNETEIMNSIYRATALLVANKEESKREILHLIAWCCVDRLTVPIIECAIECWQWLLSSRPNLNVSCMSEITSAWQATIDHRMGVFAPDKDEGDPLAVSEDHIPHPAPPCCKPHSLWIKFLQHRLDVVKYSSVEETMIIVGLVHRTLPILQPGCGNYLSRHISSVGARCDLLSLSFTMLHSVDVNASKLQKNFLRERIYLSMFDYFCSPRKFPAQKSDELRNDINSCLRLWHFVHTDRKHLVPDRGSPGITPMSGTLRKVPSHTALSGLSGGSGGKGTVPAGMNLLHGTPSMKSFQVDTLSPDLASLSSATMATGGSGWMNTVPMATSRRSTYAPSKRSSKKGKGETIAAREMMKKRTLLMYLVTAELDHLITWYNPTSGSERVVEAEATVAAWRSQEITDRQMKEMVRLSWEVSPMLCVFLTDRLRNSESLVREVSRLVRSNPQAAQHCPEAIKYLVTSQTVDSDSPELHHVVTWALGSPSTALSYFSRLFSPHPLTAQYSVKVMRKFPPEAILFYIPQLVQSIRYDTMGYVQDYLIWASGKSQLLAHQIIWNMKTNIYVDEDAHQKDPEIGSTLEQIIETIISKLSGPSQDFYRREFKFFSDVTAVSGTIKPFPKGPERTAACLEALAGIEVLEGCYLPSSPESLVLDIDRKSGTPLQSGAKAPYLAKFRVVECGISEVERLGSMTTPYELPSAENLSWQAVIFKVGDDCRQDMLALQVIQLFQNIFKQVGLDLYLFPYRVVATAPGCGVIECIPDSKSRDQLGRQTAIGMYEYFRNKYGDENSPKFQTARRNFVKSMAAYSLVLFILQIKDRHNGNIMLDQEGHLLHIDFGFMFESSPGGNLGWEPDIKLTEEMVMIMGGNMDAPSFQWFMELCVQAYLAVRPYREDIVSLVSLMLDTGLPCFRGNTIKLLRTRFQPSASPKEAAQFMVKVIRDCFLSKWSRTYDMIQYYQNQIPYY

Target ORF information:

RefSeq Version	XM_018812646.2
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis phosphatidylinositol 4-kinase alpha (LOC100178675), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018812646.2

ORF Insert Sequence:

ATGGATCAAT TCTATTTTAA GTCTGTCGTT GGTTTGGCGA AGAAATTGGC CAAGAAAAAG 
AATGCAAGAT TGGACCAGGT GAAGTTGCTG AAAGAGTTTT GTCCCAAGAT CATAAACGGG 
TCGTACCAAC TTGACAATCG TAACTACTTT GGAATTATTG CTCTAAGCGT TTATATTGTT 
GAATCTAAAC TTGAGCACCA TGAAACATTG GTTCCTTACT TGGTTGATTT ATTGCGATAT 
CTACCTTCAG CTGCATGGAG GAAATGTAAT GTGAATAAAC AAGAGCAACT CCCACTCCCA 
GAAAGATTTG CATTTTGTTT AACAACCGTC CTTAGTGATG TCATAATCAT CTCGTTGTCG 
GGTGAATACA AAGTCAGTTA CCATGACGAT ATAACAACAG CGTTTGTTGA TACTATTACA 
TCACTATGCA AAGTGTCAAT CTCGTCAAAT ATCACTCAAG CTACTTTATA CAGCATCGTA 
CTCCCAACAT TACTTGGTTT GTTACGTGGT TTCACCCGCA CACCCAACCA CCCCATGTTT 
ACCTCTGTGT TTGGTGTGGA TGGTAGTGAA TCAGGGGAAA CACCACATAG TATGATGGGG 
AAGCCCCCAC ATAGAGATGA TTACAGAACA AAACGTTCCA GTAACTTAAT GTCGACCCCT 
GGAAAGCATC AACATAGAAA CAACATTTCA TTCATGGATG GCGACAATGG ATTCAATTGG 
TTCACTGTCA AACAAGCGGG GCAAATTATT GACGTTCTGA AAAATTTGTT ACAACCATCT 
GTGTTAAACA ATCTTGATGT GACTCTCTCA TCGATGGGGA ATGACCCACA GGGGAAATAC 
CCCCCACTAC ACTATTCATC TATATCCGAA GTGTTGGTTT TGTGCGCTGC AAAGTTCCTT 
AGAGATGTAA TTAACGTTAA TGAACTCGAT GGTGTAAAAT GCAAGGAATA CAGCAGTGTT 
GCCGAATCTA GTTTTAGCCA TGGATATTCA AAACTAAGCG TAATGTTACA GAATAAAGAG 
GATCTATTAC AACATAAACT ACAAAGGTTT CTTATGCAGT GCAGATCATG CGCTGCATGC 
CTTGATGCGA TACTTGTCGT TCACACAGAA GATAATGGGG GTTCGCAAAG TTTGTTTGAG 
AAGATGACGT CAAGGTTATT ATCATCCGAG TCAAGTCATG TCATATTGGC GCATTTGCCT 
TTGCTGCATG CGTGTTTAAA GGGCTTAGGG GTTCTAGCGA GATCTTCCCC AAGCCAAGTT 
TCCCAAATAC TTTCAGTGTT GCGCGAGTTC TTACTCAAAC CAGCACCTGC GTTGGTTAAA 
CTCAAGAAAC TTGAGAAATC GGCACCACTG GGACTTAATA ACACGGAGAC ATTAGGGAGA 
AAGGCATCCC ACACCTCTCT GATGTCATCG TTTGAAGACC AAGAGAAAGC TTCGTATGCT 
TATATTAAAA CTGTTGCCAT TCAAAGCATT TGTAGATCCT TGGAAGTTGG TTGCGAAAGT 
GACCACGATT GCGTTCCAGC TTTTCTATCC TCGCTTTCAA ACGATTTGTT CTCACCCGTC 
AAGCACGAGA GCACCAAACA CTTGGTGAAG ACACACTGCA TTGATCTAAT GTCACGTATT 
GGTGTCGCCC ATGGTTTGTC CAATCATCTC ATTATACAAC AGGTTGTCAG TACATTACAG 
CAAAGGTTGA ACTCCCCATC GTCACCTTTG GATAATCTTA TCATTGATCA ACTTGCTTCA 
ATAGCTTTGC TTGGTCCGGA CAAACTATAC CAGGAGATAT TAAACATGTT GTTGCAACTT 
TGTGTGAAAG CAAGCTCAGC TAAATATGGA GTTAAGAACG ATAACGATGC TGCTAGCTTC 
AGGCATTGTT CACTTGCGAC AATCAACGCA CTAGCCAAGA TAGCTTTGAA CTTAGACAAG 
GGAAATCCCC CAGTGGTGCA AACCCAGGGA ACCCCAATTC GTTCTCCTGC AATATTTGCT 
GACTCGCCAT TAAAAAGTCC CGAGTCACCT TCAAAAGCTC CCGAGTCACC CCCAAAGTCA 
CGACTCGAAG CACTCTTGAT GAAACTTCTT GAACTTTTCG TCCAACTTTG TCTCGAAGGA 
AAGCGCTCTG TTGACCGTGT TGGGACCACT AATGGAGGAC AGGTGTCGGG TATATTGAAG 
GCTTCTAACA GCGCTGGGAA CTTGGGTGTT CTTATCCCCA TTATAGCAGT GCTTCTCCGT 
CGTCTACCCC CCATCTACTC ACCAACACCA AGGCTTCATA AACTCTTCAG GGATTTCTGG 
CTTTACTGCG TCGTGTTTGG ATTTGCTACT GAACCAGATG GTTACACCCA AAGCTTCTGG 
CCACCTGAAT GGCACAACGC TGCTTGTGAG ATATCGGTAA AGTCACCCCT TCTAATATTC 
CCTGCTGGTG ATCCATTGAG GAATGGATTG CGTTACAACT CAGCATTGAA GAATGACACA 
GTAACACCGG GCGAACTAAA CGAACTACGA ACCTTAATCT CGAACCTTCT TGGCAACTCT 
GCTGAAATAT CGGGTCTGAT AAACTCGATG GACTTTGCTT TATGTACATA TCTACTGTCT 
GTATATAGAT TAGAGAGATT GAGAGTGCTA CACAGTGTTG AGTATTCCCC ACACGTATTA 
TTCACGTACC TAGAAGACCA AACCATTCAA AAAGATAAAT CACAAATGTG GAAGTGCATC 
AAATCTGTGG CGGATTGTGT GTTTGCTGTT TTCCTGGATA TAATTGCTTC CAAGGTTAAA 
ACCTCCAGCA CAGAAACACT ATTGGTTGAA CAAGCCCAGT TTCTCCTTAT AAAGTTCAAC 
AGCGCACAGA ATAGAATACG ACAAGTGGCT GACAGCTACT TATCCCGATT AGTGGATCGC 
TTCCCCCATG TTTTATGGAA CCAAAGATTT CTATTTACGA TGCTAAACCT TCTGCACCAG 
CTCTCGTCCA CTCTGGTTCC CCACCGTTTC TCCGAGATCC CCCCCATCCT AATCAACGGC 
ACCCACCACC ACCTCCATTT GCAAGACTCT CGTAAGATGC GAGAGAAACT CGTCGAGGAT 
TTCACCAAAC ATTGTCGTCA CATCCTCAAG GAGGCGCTAA AGTGGGCGCC TGCTACTACA 
GCGAGTCATC TGCAGAACTA CCTACTACAA GATGAAACAA CTACAACACA CAACAAGCAC 
GTTGGCGTGT CTGTGGCAGC GGAAGCTTTA TACAGCAACA AACAAACGGA AAAACCAAAT 
ATTTCGGTCG GCCACCGGAG GAATAAATAT CTTGGGGAGA TTTCTGGTAT GATTAAACTG 
GGTAAATCAT CACAATTGAA CCTTTGTGAT GTCATAATAA AAGAATACAA GAGTGCATTG 
AAAGAAAACA ATGAAACTGA AATTATGAAT TCGATATACC GAGCAACAGC ACTGCTAGTG 
GCGAATAAAG AAGAAAGCAA GCGCGAGATT CTGCATTTAA TCGCATGGTG TTGTGTAGAT 
AGACTAACAG TTCCCATCAT CGAATGCGCC ATCGAGTGTT GGCAATGGCT GTTATCCTCA 
CGTCCTAACC TCAACGTATC ATGTATGAGT GAAATAACCT CAGCATGGCA GGCAACCATT 
GACCATAGAA TGGGTGTATT CGCCCCAGAT AAGGACGAAG GGGACCCACT AGCGGTGTCT 
GAGGACCACA TACCCCACCC CGCACCTCCG TGTTGTAAAC CACATTCACT GTGGATTAAG 
TTTCTCCAGC ATAGATTAGA CGTGGTAAAA TACAGCAGTG TTGAAGAAAC CATGATCATA 
GTGGGTTTAG TGCATCGTAC GTTACCCATA CTACAACCTG GTTGTGGAAA CTATCTGTCA 
AGACACATAT CATCAGTTGG GGCGCGATGT GACCTTCTTA GCCTATCCTT CACCATGCTA 
CACAGTGTGG ATGTGAATGC GTCAAAACTG CAGAAGAATT TCTTAAGGGA AAGGATCTAT 
CTTTCAATGT TCGACTATTT CTGCTCTCCG CGTAAATTTC CGGCCCAAAA ATCCGACGAG 
CTTCGTAACG ACATCAACTC ATGTTTGCGG TTGTGGCATT TCGTCCACAC CGATCGTAAG 
CACCTCGTGC CCGATCGTGG GTCCCCTGGT ATCACCCCGA TGAGTGGGAC CCTTAGAAAG 
GTACCCAGTC ATACAGCGCT GTCGGGGTTA TCCGGGGGCA GTGGGGGTAA AGGGACAGTC 
CCAGCGGGGA TGAATCTGTT ACATGGAACG CCCTCAATGA AAAGTTTCCA AGTTGACACC 
TTGAGCCCGG ACCTCGCATC CTTGTCGAGC GCGACTATGG CCACCGGAGG CAGTGGTTGG 
ATGAATACGG TTCCCATGGC AACCAGTAGA AGGTCCACGT ATGCTCCGAG TAAGAGGAGT 
TCGAAGAAAG GGAAGGGTGA AACGATAGCC GCCCGTGAAA TGATGAAGAA ACGCACCCTT 
CTAATGTATT TAGTGACAGC TGAGTTGGAT CACCTTATAA CATGGTATAA TCCAACATCT 
GGTAGTGAGA GGGTGGTGGA AGCTGAAGCT ACTGTGGCTG CATGGAGGTC GCAGGAGATC 
ACTGACCGTC AGATGAAAGA AATGGTTCGT TTATCATGGG AGGTATCACC CATGCTGTGC 
GTGTTTCTCA CCGATAGGTT GCGGAACTCA GAATCGCTCG TGCGTGAAGT ATCAAGGTTG 
GTCAGGAGCA ACCCACAGGC TGCCCAACAT TGTCCCGAGG CTATCAAATA CCTTGTGACA 
TCACAGACGG TTGATTCTGA TTCTCCAGAG CTCCACCATG TGGTAACATG GGCGTTGGGT 
TCGCCAAGTA CCGCACTGTC CTATTTTTCA AGGTTATTCA GTCCCCACCC ACTAACAGCT 
CAATACTCGG TGAAAGTGAT GCGAAAGTTT CCACCCGAAG CGATTTTGTT CTACATTCCC 
CAACTTGTAC AATCCATAAG ATACGACACG ATGGGTTACG TACAAGATTA CCTTATTTGG 
GCATCAGGTA AATCACAATT GTTGGCACAT CAAATTATTT GGAACATGAA GACAAACATA 
TATGTGGATG AAGATGCTCA TCAGAAGGAC CCAGAGATAG GCTCCACACT TGAGCAAATT 
ATTGAAACAA TTATCTCCAA ACTATCGGGG CCCTCGCAAG ACTTTTATCG TCGTGAATTC 
AAGTTTTTCT CAGATGTTAC AGCGGTTTCT GGTACGATTA AACCTTTCCC TAAAGGCCCA 
GAGCGAACGG CTGCGTGTTT GGAAGCGCTA GCTGGTATTG AGGTGTTAGA GGGTTGCTAC 
CTCCCCAGTT CCCCCGAGTC CCTGGTGCTC GACATCGACC GTAAGAGTGG AACCCCGTTA 
CAAAGCGGGG CTAAAGCTCC TTATTTGGCA AAGTTCCGGG TCGTCGAGTG CGGGATCAGT 
GAAGTGGAAC GACTCGGTTC AATGACAACC CCATATGAAT TGCCGAGCGC CGAGAACTTA 
TCGTGGCAAG CCGTGATATT CAAAGTAGGC GACGACTGTA GGCAAGACAT GCTCGCGTTA 
CAAGTTATTC AGCTGTTTCA GAATATCTTT AAACAGGTAG GGTTGGATTT ATATTTATTC 
CCGTATCGAG TGGTGGCCAC CGCACCCGGT TGTGGCGTGA TTGAGTGCAT CCCTGATTCA 
AAGTCAAGAG ACCAACTTGG AAGGCAAACC GCTATCGGGA TGTACGAATA CTTCCGCAAC 
AAATACGGCG ACGAGAATTC TCCCAAGTTC CAAACAGCTC GTCGTAACTT TGTGAAGAGC 
ATGGCAGCTT ATAGCTTGGT GTTGTTTATA CTTCAGATTA AGGACCGTCA CAATGGCAAC 
ATAATGTTGG ACCAAGAAGG CCACCTCCTC CACATAGACT TCGGGTTCAT GTTCGAAAGT 
AGTCCCGGGG GCAACCTTGG ATGGGAACCC GATATTAAAC TTACTGAGGA GATGGTGATG 
ATAATGGGGG GGAATATGGA TGCACCCTCG TTCCAGTGGT TCATGGAGTT GTGCGTCCAA 
GCGTATCTCG CTGTACGACC GTACCGTGAA GACATTGTGT CGTTGGTGTC CCTCATGCTG 
GACACGGGAC TCCCATGTTT CCGTGGCAAC ACCATTAAGT TGTTAAGGAC GAGGTTTCAA 
CCATCGGCTT CGCCCAAGGA GGCCGCGCAG TTCATGGTTA AGGTTATCCG TGATTGCTTT 
TTAAGCAAAT GGAGCCGAAC GTACGATATG ATCCAGTATT ACCAAAACCA GATTCCTTAT 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100178675 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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