COL4A5 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL4A5
Entrez Gene ID	103232468
Full Name	collagen type IV alpha 5 chain
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007992528.1	XP_007990719.1	collagen alpha-5(IV) chain isoform X1
XM_007992531.1	XP_007990722.1	collagen alpha-5(IV) chain isoform X4
XM_007992532.1	XP_007990723.1	collagen alpha-5(IV) chain isoform X5
XM_007992527.1	XP_007990718.1	collagen alpha-5(IV) chain isoform X1
XM_007992529.1	XP_007990720.1	collagen alpha-5(IV) chain isoform X2
XM_007992530.1	XP_007990721.1	collagen alpha-5(IV) chain isoform X3

Pathways from BioSystems
Gene Ontology

The following COL4A5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCh126035	XM_007992528.1 Latest version!	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh126035	XM_007992527.1 Latest version!	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh126036	XM_007992529.1 Latest version!	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh126037	XM_007992530.1 Latest version!	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh126038	XM_007992531.1 Latest version!	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCh126039	XM_007992532.1 Latest version!	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL4A5 ( XM_007992528.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007990719.1 Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCh126035
Clone ID Related Accession (Same CDS sequence)	XM_007992528.1 , XM_007992527.1
Accession Version	XM_007992528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735280.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTACCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
GTACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGACCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAACGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACATTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGCTTGCC CGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGC 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTGTGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGGCTTC CGGGGCCTCC TGGCCCTCCT 
GGACCTCACA TCCCTCCTAG TGATGAGATA TGTGAAGCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC CCTTGGTTTC CCTGGACAGA AAGGTGAAAA AGGACAAGCT 
GGTGCAACTG GTTCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCTC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCTCCTG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCAGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGACCCTG GACTCCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCTGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAACG CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCAG GTCCTCCAGG ACGCACTGGT GTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCTGGAGCA CCTGGTTCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGCTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGC GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTCC TGGAGTTCCT GGACAGCCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCTGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA AACTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGAATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCATGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CCGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC CCCAGGTCTC 
CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAACCCAG GCCAACCTGG GCTACCTGGC 
TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGTCGGCCG 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG 
AAAGGACCCA GTGGAATACC TGGATCAGCT GGTCCTGAGG GGGAACCGGG ACTTACTGGT 
CCTCCAGGTC CTCCTGGATT ACCTGGTCCC TCAGGACAGA GTATCATAAT CAAAGGAGAT 
GCTGGTCCTC CAGGAATCCC TGGACAACCT GGGTTAAAAG GTCTACCAGG ACCCCAAGGA 
CCTCAGGGTT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGCACC 
CGTGGTTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA 
ACCTCCTCTA TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA 
CAATGCCCGC AGGGAACACT TCAGATCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCATGGCCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT 
ACCATGCCTT TTATGTTCTG CAACATCAAT AATGTTTGCA ACTTCGCTTC AAGAAATGAC 
TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAGTGA GCATGCAACC CCTAAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC 
GCAGTTCACA GTCAGACGAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTGTGG 
ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA 
GCCTCTCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG 
AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG 
TCAGACATGT TCAGTAAACC TCAGTCAGAA ACACTGAAAG CAGGAGACTT GAGGACACGA 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007990719.1
CDS	171..5246
Translation	MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGVQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVVGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPPGPHIPPSDEICEAGPPGPPGSPGDKGLQGEQGMKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGSKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGSPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGTLGPKGDRGFPGPPGPPGRTGVDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGSIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPPGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGETGPVGGGGRPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGIPGSAGPEGEPGLTGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTLQIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPVSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_007992528.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007992528.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTACCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
GTACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGACCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAACGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACATTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGCTTGCC CGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGC 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTGTGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGGCTTC CGGGGCCTCC TGGCCCTCCT 
GGACCTCACA TCCCTCCTAG TGATGAGATA TGTGAAGCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC CCTTGGTTTC CCTGGACAGA AAGGTGAAAA AGGACAAGCT 
GGTGCAACTG GTTCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCTC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCTCCTG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCAGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGACCCTG GACTCCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCTGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAACG CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCAG GTCCTCCAGG ACGCACTGGT GTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCTGGAGCA CCTGGTTCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGCTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGC GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTCC TGGAGTTCCT GGACAGCCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCTGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA AACTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGAATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCATGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CCGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC CCCAGGTCTC 
CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAACCCAG GCCAACCTGG GCTACCTGGC 
TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGTCGGCCG 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG 
AAAGGACCCA GTGGAATACC TGGATCAGCT GGTCCTGAGG GGGAACCGGG ACTTACTGGT 
CCTCCAGGTC CTCCTGGATT ACCTGGTCCC TCAGGACAGA GTATCATAAT CAAAGGAGAT 
GCTGGTCCTC CAGGAATCCC TGGACAACCT GGGTTAAAAG GTCTACCAGG ACCCCAAGGA 
CCTCAGGGTT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGCACC 
CGTGGTTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA 
ACCTCCTCTA TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA 
CAATGCCCGC AGGGAACACT TCAGATCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCATGGCCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT 
ACCATGCCTT TTATGTTCTG CAACATCAAT AATGTTTGCA ACTTCGCTTC AAGAAATGAC 
TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAGTGA GCATGCAACC CCTAAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC 
GCAGTTCACA GTCAGACGAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTGTGG 
ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA 
GCCTCTCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG 
AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG 
TCAGACATGT TCAGTAAACC TCAGTCAGAA ACACTGAAAG CAGGAGACTT GAGGACACGA 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_007992527.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007990718.1 Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X1, mRNA.

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CloneID	OCh126035
Clone ID Related Accession (Same CDS sequence)	XM_007992528.1 , XM_007992527.1
Accession Version	XM_007992527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735280.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTACCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
GTACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGACCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAACGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACATTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGCTTGCC CGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGC 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTGTGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGGCTTC CGGGGCCTCC TGGCCCTCCT 
GGACCTCACA TCCCTCCTAG TGATGAGATA TGTGAAGCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC CCTTGGTTTC CCTGGACAGA AAGGTGAAAA AGGACAAGCT 
GGTGCAACTG GTTCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCTC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCTCCTG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCAGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGACCCTG GACTCCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCTGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAACG CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCAG GTCCTCCAGG ACGCACTGGT GTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCTGGAGCA CCTGGTTCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGCTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGC GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTCC TGGAGTTCCT GGACAGCCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCTGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA AACTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGAATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCATGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CCGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC CCCAGGTCTC 
CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAACCCAG GCCAACCTGG GCTACCTGGC 
TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGTCGGCCG 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG 
AAAGGACCCA GTGGAATACC TGGATCAGCT GGTCCTGAGG GGGAACCGGG ACTTACTGGT 
CCTCCAGGTC CTCCTGGATT ACCTGGTCCC TCAGGACAGA GTATCATAAT CAAAGGAGAT 
GCTGGTCCTC CAGGAATCCC TGGACAACCT GGGTTAAAAG GTCTACCAGG ACCCCAAGGA 
CCTCAGGGTT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGCACC 
CGTGGTTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA 
ACCTCCTCTA TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA 
CAATGCCCGC AGGGAACACT TCAGATCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCATGGCCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT 
ACCATGCCTT TTATGTTCTG CAACATCAAT AATGTTTGCA ACTTCGCTTC AAGAAATGAC 
TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAGTGA GCATGCAACC CCTAAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC 
GCAGTTCACA GTCAGACGAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTGTGG 
ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA 
GCCTCTCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG 
AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG 
TCAGACATGT TCAGTAAACC TCAGTCAGAA ACACTGAAAG CAGGAGACTT GAGGACACGA 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007990718.1
CDS	192..5267
Translation	MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGVQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVVGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPPGPHIPPSDEICEAGPPGPPGSPGDKGLQGEQGMKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGSKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGSPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGTLGPKGDRGFPGPPGPPGRTGVDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGSIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPPGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGETGPVGGGGRPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGIPGSAGPEGEPGLTGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTLQIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPVSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_007992527.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007992527.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTACCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
GTACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGACCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAACGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACATTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGCTTGCC CGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGC 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTGTGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGGCTTC CGGGGCCTCC TGGCCCTCCT 
GGACCTCACA TCCCTCCTAG TGATGAGATA TGTGAAGCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC CCTTGGTTTC CCTGGACAGA AAGGTGAAAA AGGACAAGCT 
GGTGCAACTG GTTCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCTC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCTCCTG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCAGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGACCCTG GACTCCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCTGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAACG CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCAG GTCCTCCAGG ACGCACTGGT GTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCTGGAGCA CCTGGTTCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGCTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGC GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTCC TGGAGTTCCT GGACAGCCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCTGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA AACTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGAATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCATGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CCGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC CCCAGGTCTC 
CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAACCCAG GCCAACCTGG GCTACCTGGC 
TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGTCGGCCG 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG 
AAAGGACCCA GTGGAATACC TGGATCAGCT GGTCCTGAGG GGGAACCGGG ACTTACTGGT 
CCTCCAGGTC CTCCTGGATT ACCTGGTCCC TCAGGACAGA GTATCATAAT CAAAGGAGAT 
GCTGGTCCTC CAGGAATCCC TGGACAACCT GGGTTAAAAG GTCTACCAGG ACCCCAAGGA 
CCTCAGGGTT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGCACC 
CGTGGTTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA 
ACCTCCTCTA TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA 
CAATGCCCGC AGGGAACACT TCAGATCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCATGGCCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT 
ACCATGCCTT TTATGTTCTG CAACATCAAT AATGTTTGCA ACTTCGCTTC AAGAAATGAC 
TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAGTGA GCATGCAACC CCTAAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC 
GCAGTTCACA GTCAGACGAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTGTGG 
ATTGGTTATT CCTTCATGAT GCATACAAGT GCAGGGGCAG AAGGCTCAGG TCAAGCCCTA 
GCCTCTCCTG GTTCCTGCTT GGAAGAGTTT CGTTCAGCTC CCTTCATCGA ATGTCATGGG 
AGGGGTACCT GTAACTACTA TGCCAACTCC TACAGCTTTT GGCTGGCAAC TGTAGATGTG 
TCAGACATGT TCAGTAAACC TCAGTCAGAA ACACTGAAAG CAGGAGACTT GAGGACACGA 
ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_007992529.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007990720.1 Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X3, mRNA.

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CloneID	OCh126036
Clone ID Related Accession (Same CDS sequence)	XM_007992529.1
Accession Version	XM_007992529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735280.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTACCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
GTACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGACCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAACGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACATTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGCTTGCC CGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGC 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTGTGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGGCTTC CGGGGCCTCC TGGCCCTCCT 
GGACCTCACA TCCCTCCTAG TGATGAGATA TGTGAAGCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC CCTTGGTTTC CCTGGACAGA AAGGTGAAAA AGGACAAGCT 
GGTGCAACTG GTTCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCTC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCTCCTG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCAGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGACCCTG GACTCCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCTGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAACG CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCAG GTCCTCCAGG ACGCACTGGT GTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCTGGAGCA CCTGGTTCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGCTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGC GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTCC TGGAGTTCCT GGACAGCCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCTGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA AACTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGAATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCATGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CCGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTTTTCAAGG TCTACCAGGT CCAGAAGGTC CCCCAGGTCT CCCTGGAAAT 
GGAGGTATTA AAGGAGAGAA GGGAAACCCA GGCCAACCTG GGCTACCTGG CTTGCCTGGT 
TTGAAAGGAG ATCAAGGACC ACCAGGACTC CAGGGTAATC CTGGTCGGCC GGGTCTCAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGCAT GAAAGGACCC 
AGTGGAATAC CTGGATCAGC TGGTCCTGAG GGGGAACCGG GACTTACTGG TCCTCCAGGT 
CCTCCTGGAT TACCTGGTCC CTCAGGACAG AGTATCATAA TCAAAGGAGA TGCTGGTCCT 
CCAGGAATCC CTGGACAACC TGGGTTAAAA GGTCTACCAG GACCCCAAGG ACCTCAGGGT 
TTACCAGGTC CAACTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGT CTGCCAGGAC AGCCAGGCAC CCGTGGTTTG 
GATGGTCCCC CCGGGCCAGA TGGATTGCAA GGTCCCCCAG GTCCCCCTGG AACCTCCTCT 
ATTGCACATG GATTTCTTAT TACACGCCAC AGCCAGACAA CGGATGCACC ACAATGCCCG 
CAGGGAACAC TTCAGATCTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCCCATGGCC AAGACTTGGG GACGGCTGGC AGCTGCCTTC GTCGCTTTAG TACCATGCCT 
TTTATGTTCT GCAACATCAA TAATGTTTGC AACTTCGCTT CAAGAAATGA CTATTCTTAC 
TGGCTCTCTA CCCCAGAGCC CATGCCAGTG AGCATGCAAC CCCTAAAGGG CCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCAG CTGTGGTGAT CGCAGTTCAC 
AGTCAGACGA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTGTG GATTGGTTAT 
TCCTTCATGA TGCATACAAG TGCAGGGGCA GAAGGCTCAG GTCAAGCCCT AGCCTCTCCT 
GGTTCCTGCT TGGAAGAGTT TCGTTCAGCT CCCTTCATCG AATGTCATGG GAGGGGTACC 
TGTAACTACT ATGCCAACTC CTACAGCTTT TGGCTGGCAA CTGTAGATGT GTCAGACATG 
TTCAGTAAAC CTCAGTCAGA AACACTGAAA GCAGGAGACT TGAGGACACG AATTAGCCGA 
TGTCAAGTGT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007990720.1
CDS	192..5258
Translation	MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGVQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVVGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPPGPHIPPSDEICEAGPPGPPGSPGDKGLQGEQGMKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGSKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGSPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGTLGPKGDRGFPGPPGPPGRTGVDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGSIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPPGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGETGPVGGGGRPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGIPGSAGPEGEPGLTGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTLQIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPVSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_007992529.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007992529.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTACCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
GTACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGACCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAACGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACATTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGCTTGCC CGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGC 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTGTGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGGCTTC CGGGGCCTCC TGGCCCTCCT 
GGACCTCACA TCCCTCCTAG TGATGAGATA TGTGAAGCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC CCTTGGTTTC CCTGGACAGA AAGGTGAAAA AGGACAAGCT 
GGTGCAACTG GTTCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCTC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCTCCTG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCAGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGACCCTG GACTCCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCTGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAACG CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCAG GTCCTCCAGG ACGCACTGGT GTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCTGGAGCA CCTGGTTCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGCTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGC GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTCC TGGAGTTCCT GGACAGCCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCTGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA AACTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGAATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCATGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CCGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTTTTCAAGG TCTACCAGGT CCAGAAGGTC CCCCAGGTCT CCCTGGAAAT 
GGAGGTATTA AAGGAGAGAA GGGAAACCCA GGCCAACCTG GGCTACCTGG CTTGCCTGGT 
TTGAAAGGAG ATCAAGGACC ACCAGGACTC CAGGGTAATC CTGGTCGGCC GGGTCTCAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGCAT GAAAGGACCC 
AGTGGAATAC CTGGATCAGC TGGTCCTGAG GGGGAACCGG GACTTACTGG TCCTCCAGGT 
CCTCCTGGAT TACCTGGTCC CTCAGGACAG AGTATCATAA TCAAAGGAGA TGCTGGTCCT 
CCAGGAATCC CTGGACAACC TGGGTTAAAA GGTCTACCAG GACCCCAAGG ACCTCAGGGT 
TTACCAGGTC CAACTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGT CTGCCAGGAC AGCCAGGCAC CCGTGGTTTG 
GATGGTCCCC CCGGGCCAGA TGGATTGCAA GGTCCCCCAG GTCCCCCTGG AACCTCCTCT 
ATTGCACATG GATTTCTTAT TACACGCCAC AGCCAGACAA CGGATGCACC ACAATGCCCG 
CAGGGAACAC TTCAGATCTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA 
GCCCATGGCC AAGACTTGGG GACGGCTGGC AGCTGCCTTC GTCGCTTTAG TACCATGCCT 
TTTATGTTCT GCAACATCAA TAATGTTTGC AACTTCGCTT CAAGAAATGA CTATTCTTAC 
TGGCTCTCTA CCCCAGAGCC CATGCCAGTG AGCATGCAAC CCCTAAAGGG CCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCAG CTGTGGTGAT CGCAGTTCAC 
AGTCAGACGA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTGTG GATTGGTTAT 
TCCTTCATGA TGCATACAAG TGCAGGGGCA GAAGGCTCAG GTCAAGCCCT AGCCTCTCCT 
GGTTCCTGCT TGGAAGAGTT TCGTTCAGCT CCCTTCATCG AATGTCATGG GAGGGGTACC 
TGTAACTACT ATGCCAACTC CTACAGCTTT TGGCTGGCAA CTGTAGATGT GTCAGACATG 
TTCAGTAAAC CTCAGTCAGA AACACTGAAA GCAGGAGACT TGAGGACACG AATTAGCCGA 
TGTCAAGTGT GCATGAAGAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_007992530.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007990721.1 Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X4, mRNA.

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CloneID	OCh126037
Clone ID Related Accession (Same CDS sequence)	XM_007992530.1
Accession Version	XM_007992530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	collagen alpha-5(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735280.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTACCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
GTACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGACCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAACGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACATTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGCTTGCC CGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGC 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTGTGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGGCTTC CGGGGCCTCC TGGCCCTCCT 
GGACCTCACA TCCCTCCTAG TGATGAGATA TGTGAAGCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC CCTTGGTTTC CCTGGACAGA AAGGTGAAAA AGGACAAGCT 
GGTGCAACTG GTTCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCTC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCTCCTG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCAGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGACCCTG GACTCCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCTGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAACG CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCAG GTCCTCCAGG ACGCACTGGT GTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCTGGAGCA CCTGGTTCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGCTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGC GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTCC TGGAGTTCCT GGACAGCCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCTGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA AACTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGAATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCATGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CCGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCCCCAGGTC TCCCTGGAAA TGGAGGTATT 
AAAGGAGAGA AGGGAAACCC AGGCCAACCT GGGCTACCTG GCTTGCCTGG TTTGAAAGGA 
GATCAAGGAC CACCAGGACT CCAGGGTAAT CCTGGTCGGC CGGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGCA TGAAAGGACC CAGTGGAATA 
CCTGGATCAG CTGGTCCTGA GGGGGAACCG GGACTTACTG GTCCTCCAGG TCCTCCTGGA 
TTACCTGGTC CCTCAGGACA GAGTATCATA ATCAAAGGAG ATGCTGGTCC TCCAGGAATC 
CCTGGACAAC CTGGGTTAAA AGGTCTACCA GGACCCCAAG GACCTCAGGG TTTACCAGGT 
CCAACTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG TCTGCCAGGA CAGCCAGGCA CCCGTGGTTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA AGGTCCCCCA GGTCCCCCTG GAACCTCCTC TATTGCACAT 
GGATTTCTTA TTACACGCCA CAGCCAGACA ACGGATGCAC CACAATGCCC GCAGGGAACA 
CTTCAGATCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCATGGC 
CAAGACTTGG GGACGGCTGG CAGCTGCCTT CGTCGCTTTA GTACCATGCC TTTTATGTTC 
TGCAACATCA ATAATGTTTG CAACTTCGCT TCAAGAAATG ACTATTCTTA CTGGCTCTCT 
ACCCCAGAGC CCATGCCAGT GAGCATGCAA CCCCTAAAGG GCCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCA GCTGTGGTGA TCGCAGTTCA CAGTCAGACG 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTGT GGATTGGTTA TTCCTTCATG 
ATGCATACAA GTGCAGGGGC AGAAGGCTCA GGTCAAGCCC TAGCCTCTCC TGGTTCCTGC 
TTGGAAGAGT TTCGTTCAGC TCCCTTCATC GAATGTCATG GGAGGGGTAC CTGTAACTAC 
TATGCCAACT CCTACAGCTT TTGGCTGGCA ACTGTAGATG TGTCAGACAT GTTCAGTAAA 
CCTCAGTCAG AAACACTGAA AGCAGGAGAC TTGAGGACAC GAATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007990721.1
CDS	192..5249
Translation	MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGVQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVVGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPPGPHIPPSDEICEAGPPGPPGSPGDKGLQGEQGMKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGSKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGSPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGTLGPKGDRGFPGPPGPPGRTGVDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGSIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPPGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGETGPVGGGGRPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGIPGSAGPEGEPGLTGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTLQIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPVSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_007992530.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007992530.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTACCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
GTACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGACCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAACGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACATTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGCTTGCC CGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGC 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTGTGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGGCTTC CGGGGCCTCC TGGCCCTCCT 
GGACCTCACA TCCCTCCTAG TGATGAGATA TGTGAAGCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC CCTTGGTTTC CCTGGACAGA AAGGTGAAAA AGGACAAGCT 
GGTGCAACTG GTTCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCTC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCTCCTG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCAGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGACCCTG GACTCCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCTGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAACG CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCAG GTCCTCCAGG ACGCACTGGT GTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCTGGAGCA CCTGGTTCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGCTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGC GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTCC TGGAGTTCCT GGACAGCCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCTGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA AACTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGAATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCATGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CCGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCTACCAGG TCCAGAAGGT CCCCCAGGTC TCCCTGGAAA TGGAGGTATT 
AAAGGAGAGA AGGGAAACCC AGGCCAACCT GGGCTACCTG GCTTGCCTGG TTTGAAAGGA 
GATCAAGGAC CACCAGGACT CCAGGGTAAT CCTGGTCGGC CGGGTCTCAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGCA TGAAAGGACC CAGTGGAATA 
CCTGGATCAG CTGGTCCTGA GGGGGAACCG GGACTTACTG GTCCTCCAGG TCCTCCTGGA 
TTACCTGGTC CCTCAGGACA GAGTATCATA ATCAAAGGAG ATGCTGGTCC TCCAGGAATC 
CCTGGACAAC CTGGGTTAAA AGGTCTACCA GGACCCCAAG GACCTCAGGG TTTACCAGGT 
CCAACTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG TCTGCCAGGA CAGCCAGGCA CCCGTGGTTT GGATGGTCCC 
CCCGGGCCAG ATGGATTGCA AGGTCCCCCA GGTCCCCCTG GAACCTCCTC TATTGCACAT 
GGATTTCTTA TTACACGCCA CAGCCAGACA ACGGATGCAC CACAATGCCC GCAGGGAACA 
CTTCAGATCT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCCCATGGC 
CAAGACTTGG GGACGGCTGG CAGCTGCCTT CGTCGCTTTA GTACCATGCC TTTTATGTTC 
TGCAACATCA ATAATGTTTG CAACTTCGCT TCAAGAAATG ACTATTCTTA CTGGCTCTCT 
ACCCCAGAGC CCATGCCAGT GAGCATGCAA CCCCTAAAGG GCCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAAGCTCCA GCTGTGGTGA TCGCAGTTCA CAGTCAGACG 
ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTGT GGATTGGTTA TTCCTTCATG 
ATGCATACAA GTGCAGGGGC AGAAGGCTCA GGTCAAGCCC TAGCCTCTCC TGGTTCCTGC 
TTGGAAGAGT TTCGTTCAGC TCCCTTCATC GAATGTCATG GGAGGGGTAC CTGTAACTAC 
TATGCCAACT CCTACAGCTT TTGGCTGGCA ACTGTAGATG TGTCAGACAT GTTCAGTAAA 
CCTCAGTCAG AAACACTGAA AGCAGGAGAC TTGAGGACAC GAATTAGCCG ATGTCAAGTG 
TGCATGAAGA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_007992531.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007990722.1 Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X5, mRNA.

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CloneID	OCh126038
Clone ID Related Accession (Same CDS sequence)	XM_007992531.1
Accession Version	XM_007992531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	collagen alpha-5(IV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735280.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTACCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
GTACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGACCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAACGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACATTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGCTTGCC CGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGC 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTGTGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGGCTTC CGGGGCCTCC TGGCCCTCCT 
GGACCTCACA TCCCTCCTAG TGATGAGATA TGTGAAGCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC CCTTGGTTTC CCTGGACAGA AAGGTGAAAA AGGACAAGCT 
GGTGCAACTG GTTCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCTC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCTCCTG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCAGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGACCCTG GACTCCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCTGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAACG CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCAG GTCCTCCAGG ACGCACTGGT GTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCTGGAGCA CCTGGTTCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGCTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGC GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTCC TGGAGTTCCT GGACAGCCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCTGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA AACTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGAATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCATGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CCGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC CCCAGGTCTC 
CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAACCCAG GCCAACCTGG GCTACCTGGC 
TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGTCGGCCG 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG 
AAAGGACCCA GTGGAATACC TGGATCAGCT GGTCCTGAGG GGGAACCGGG ACTTACTGGT 
CCTCCAGGTC CTCCTGGATT ACCTGGTCCC TCAGGACAGA GTATCATAAT CAAAGGAGAT 
GCTGGTCCTC CAGGAATCCC TGGACAACCT GGGTTAAAAG GTCTACCAGG ACCCCAAGGA 
CCTCAGGGTT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGCACC 
CGTGGTTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA 
ACCTCCTCTA TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA 
CAATGCCCGC AGGGAACACT TCAGATCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCATGGCCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT 
ACCATGCCTT TTATGTTCTG CAACATCAAT AATGTTTGCA ACTTCGCTTC AAGAAATGAC 
TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAGTGA GCATGCAACC CCTAAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC 
GCAGTTCACA GTCAGACGAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTGTGG 
ATTGGTTATT CCTTCATGAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007990722.1
CDS	192..5015
Translation	MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGVQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVVGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPPGPHIPPSDEICEAGPPGPPGSPGDKGLQGEQGMKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGSKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGSPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGTLGPKGDRGFPGPPGPPGRTGVDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGSIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPPGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGETGPVGGGGRPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGIPGSAGPEGEPGLTGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTLQIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPVSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMM

Target ORF information:

RefSeq Version	XM_007992531.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007992531.1

ORF Insert Sequence:

ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTTCT TACTGGCCCT GAGTCTTTGG 
GGGCAGCCTG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGC 
AGTGGCATAA AAGGGGAAAA GGGAGAGAGA GGGTTTCCAG GTTTGGAAGG ACACCCAGGA 
TTACCTGGAT TTCCAGGTCC AGAAGGGCCT CCAGGGCCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT 
ATTCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT 
GGTTTACAGG GTCCTCCAGG ACCCCCTGGG ATCCCAGGTA TGAAGGGTGA ACCAGGTAGT 
ATAATTATGT CATCACTGCC AGGACCAAAG GGTAATCCAG GATATCCAGG TCCTCCTGGA 
GTACAAGGCC TACCTGGTCC CACTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA 
GGTTTGATGG GCCCTCCTGG TCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG TGAAAAGGGT GAGCAAGGTC TTCAGGGCCC ACCTGGGCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGATGTAG AGTTTCAGAA AGGAGACCAG 
GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCTCCAGGGA TACGTGGTCC TCCAGGTCCC 
CCAGGTGGTG AGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAAAACGG CCAACCAGGA ATTCCTGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CCGGAAGGGA TGGTGAAAAG GGCCAAAAAG GTGACATTGG CCCACCTGGA 
CCTCCTGGAC TTGTAATTCC TAGACCTGGG ACTGGTATAA CTATAGGAGA AAAAGGAAAC 
ATTGGGTTGC CTGGCTTGCC CGGAGAAAAA GGAGAGCGAG GATTTCCTGG AATACAGGGC 
CCACCTGGCC TTCCTGGACC TCCAGGGGCT GCAGTTGTGG GTCCTCCTGG CCCTCCTGGA 
TTTCCTGGAG AAAGGGGTCA GAAAGGTGAT GAAGGACCAC CTGGAATTTC CATTCCTGGA 
CCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGGCTTC CGGGGCCTCC TGGCCCTCCT 
GGACCTCACA TCCCTCCTAG TGATGAGATA TGTGAAGCAG GCCCTCCAGG CCCCCCAGGA 
TCTCCAGGTG ATAAAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACT 
TGCTTCAACT GCATTGGAAC TGGTATTTCA GGGCCTCCAG GTCAACCTGG TTTGCCAGGT 
CTCCCAGGTC CTCCAGGATC CCTTGGTTTC CCTGGACAGA AAGGTGAAAA AGGACAAGCT 
GGTGCAACTG GTTCCAAAGG ATTACCAGGC ATTCCAGGAG CTCCAGGTGC TCCAGGCTTT 
CCTGGATCTA AAGGTGAACC TGGTGATATC CTCACTTTTC CAGGAATGAA GGGTGACAAA 
GGAGAGTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCTC TCCTGGACAG 
GATGGATTGC CAGGGCTTCC TGGCCCGAAA GGAGAGCCTG GTGGAATTAC TTTTAAGGGT 
GAAAGAGGTC CCCCTGGGAA CCCAGGTTTA CCAGGCCTCC CAGGGAATAT AGGGCCTATG 
GGTCCTCCTG GTTTTGGCCC TCCAGGCCCA GTAGGTGAAA AAGGCATACA AGGTGTGGCA 
GGAAATCCAG GCCAGCCAGG AATACCAGGT CCTAAAGGGG ATCCAGGTCA GACTATAACC 
CAGCCAGGGA AGCCTGGCTT GCCTGGTAAC CCAGGCAGAG ATGGTGAAGT AGGTCTTCCA 
GGTGACCCTG GACTCCCAGG GCAACCAGGC TTGCCAGGGA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGAATTGG GCTTCCTGGA CCACCTGGTC CCAAAGGCTT TCCTGGAATT 
CCAGGACCTC CAGGAGCACC TGGGACACCT GGAAGAATTG GTCTAGAAGG CCCTCCTGGG 
CCACCTGGCT TTCCAGGACC AAAGGGTGAA CCAGGATTTG CATTACCTGG GCCACCTGGG 
CCACCAGGAC TTCCAGGTTT CAAAGGAACG CTTGGTCCAA AAGGTGATCG TGGTTTCCCA 
GGACCTCCAG GTCCTCCAGG ACGCACTGGT GTAGATGGGC TCCCTGGACC AAAAGGTGAT 
GTTGGACCAA ATGGACAACC TGGACCAATG GGACCTCCTG GGCTGCCAGG AATAGGTGTT 
CAGGGACCAC CAGGACCACC AGGGATTCCT GGGCCAATAG GTCAACCTGG TTTACATGGA 
ATACCAGGAG AGAAGGGGGA TCCAGGACCT CCTGGACTTG ATGTTCCAGG ACCCCCAGGT 
GAAAGAGGCA GTCCAGGGAT CCCTGGAGCA CCTGGTTCTA TAGGACCTCC AGGATCACCA 
GGGCTTCCAG GAAAAGCAGG TGCCTCTGGA TTTCCAGGTA CCAAAGGTGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTACCTGGT 
CTTAAAGGTG ATGATGGCTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAAAAA 
GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CCTCCTGGAC CAATGGATCC AAATCTTCTG 
GGCTCAAAAG GAGAGAAGGG GGAACCTGGC TTACCAGGTA TACCTGGAGT TTCAGGGCCA 
AAAGGTTATC AGGGCTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA 
TTACCAGGAC CACCAGGTCC CAAAGGTAAC CCTGGTCTCC CTGGACAGCC AGGTCTTATA 
GGACCTCCTG GACTTAAAGG AACCATCGGC GATATGGGTT TTCCAGGGCC TCAGGGTGTG 
GAAGGGCCTC CTGGACCTCC TGGAGTTCCT GGACAGCCTG GCTCCCCAGG ATTACCTGGA 
CAGAAAGGAG ACAAAGGTGA TCCTGGTATT TCAAGCATTG GTCTTCCAGG TCTTCCTGGT 
CCAAAGGGTG AGCCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTATCAA AGGTTCTGTG 
GGAGATCCTG GTTTGCCTGG ATTACCAGGA ACCCCTGGAG CAAAAGGACA ACCAGGCCTT 
CCTGGATTCC CAGGAACCCC AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG 
AACCCTGGCC TTCCAGGAGA AACTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCAACCA 
GGGCCTCCAG GCGAAAAAGG CAAACCCGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG 
AAGGGTGAAC CAGGTCAACC AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT 
TCTGGCCAAA AGGGTGATGG AGGATTACCT GGAATTCCAG GAAATCCTGG CCTTCCAGGT 
CCAAAGGGCG AACCAGGCTT TCATGGTTTC CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT 
GGTTCTCCGG GTCCAGCTCT GGAAGGACCT AAAGGCAACC CCGGGCCCCA AGGTCCTCCT 
GGGAGACCAG GTCCTACAGG TTTTCAAGGT CTACCAGGTC CAGAAGGTCC CCCAGGTCTC 
CCTGGAAATG GAGGTATTAA AGGAGAGAAG GGAAACCCAG GCCAACCTGG GCTACCTGGC 
TTGCCTGGTT TGAAAGGAGA TCAAGGACCA CCAGGACTCC AGGGTAATCC TGGTCGGCCG 
GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGCATG 
AAAGGACCCA GTGGAATACC TGGATCAGCT GGTCCTGAGG GGGAACCGGG ACTTACTGGT 
CCTCCAGGTC CTCCTGGATT ACCTGGTCCC TCAGGACAGA GTATCATAAT CAAAGGAGAT 
GCTGGTCCTC CAGGAATCCC TGGACAACCT GGGTTAAAAG GTCTACCAGG ACCCCAAGGA 
CCTCAGGGTT TACCAGGTCC AACTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGTC TGCCAGGACA GCCAGGCACC 
CGTGGTTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAAG GTCCCCCAGG TCCCCCTGGA 
ACCTCCTCTA TTGCACATGG ATTTCTTATT ACACGCCACA GCCAGACAAC GGATGCACCA 
CAATGCCCGC AGGGAACACT TCAGATCTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA 
AATAAAAGAG CCCATGGCCA AGACTTGGGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT 
ACCATGCCTT TTATGTTCTG CAACATCAAT AATGTTTGCA ACTTCGCTTC AAGAAATGAC 
TATTCTTACT GGCTCTCTAC CCCAGAGCCC ATGCCAGTGA GCATGCAACC CCTAAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCAGC TGTGGTGATC 
GCAGTTCACA GTCAGACGAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTGTGG 
ATTGGTTATT CCTTCATGAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A5 ( XM_007992532.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007990723.1 Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X6, mRNA.

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CloneID	OCh126039
Clone ID Related Accession (Same CDS sequence)	XM_007992532.1
Accession Version	XM_007992532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	collagen alpha-5(IV) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735280.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCC ATGATGGGGC CCCAGGACCT CAAGGTATTC CTGGATGCAA TGGAACCAAG 
GGAGAACGTG GATTTCCAGG CAGTCCCGGT TTTCCTGGTT TACAGGGTCC TCCAGGACCC 
CCTGGGATCC CAGGTATGAA GGGTGAACCA GGTAGTATAA TTATGTCATC ACTGCCAGGA 
CCAAAGGGTA ATCCAGGATA TCCAGGTCCT CCTGGAGTAC AAGGCCTACC TGGTCCCACT 
GGTATACCAG GGCCAATTGG TCCCCCAGGA CCACCAGGTT TGATGGGCCC TCCTGGTCCA 
CCAGGACTTC CAGGACCAAA GGGGAATATG GGCTTAAATT TCCAGGGACC CAAAGGTGAA 
AAGGGTGAGC AAGGTCTTCA GGGCCCACCT GGGCCACCTG GGCAGATCAG TGAACAGAAA 
AGACCAATTG ATGTAGAGTT TCAGAAAGGA GACCAGGGAC TTCCTGGTGA CCGAGGGCCT 
CCTGGACCTC CAGGGATACG TGGTCCTCCA GGTCCCCCAG GTGGTGAGAA AGGTGAGAAG 
GGTGAGCAAG GAGAGCCAGG CAAAAGAGGT AAACCAGGCA AAGATGGAGA AAACGGCCAA 
CCAGGAATTC CTGGTTTGCC TGGTGATCCT GGTTACCCTG GTGAACCCGG AAGGGATGGT 
GAAAAGGGCC AAAAAGGTGA CATTGGCCCA CCTGGACCTC CTGGACTTGT AATTCCTAGA 
CCTGGGACTG GTATAACTAT AGGAGAAAAA GGAAACATTG GGTTGCCTGG CTTGCCCGGA 
GAAAAAGGAG AGCGAGGATT TCCTGGAATA CAGGGCCCAC CTGGCCTTCC TGGACCTCCA 
GGGGCTGCAG TTGTGGGTCC TCCTGGCCCT CCTGGATTTC CTGGAGAAAG GGGTCAGAAA 
GGTGATGAAG GACCACCTGG AATTTCCATT CCTGGACCTC CTGGACTTGA TGGACAGCCT 
GGGGCTCCTG GGCTTCCGGG GCCTCCTGGC CCTCCTGGAC CTCACATCCC TCCTAGTGAT 
GAGATATGTG AAGCAGGCCC TCCAGGCCCC CCAGGATCTC CAGGTGATAA AGGACTCCAA 
GGAGAACAAG GAATGAAAGG TGACAAAGGT GACACTTGCT TCAACTGCAT TGGAACTGGT 
ATTTCAGGGC CTCCAGGTCA ACCTGGTTTG CCAGGTCTCC CAGGTCCTCC AGGATCCCTT 
GGTTTCCCTG GACAGAAAGG TGAAAAAGGA CAAGCTGGTG CAACTGGTTC CAAAGGATTA 
CCAGGCATTC CAGGAGCTCC AGGTGCTCCA GGCTTTCCTG GATCTAAAGG TGAACCTGGT 
GATATCCTCA CTTTTCCAGG AATGAAGGGT GACAAAGGAG AGTTGGGTTC CCCTGGAGCT 
CCAGGGCTTC CTGGTTTACC TGGCTCTCCT GGACAGGATG GATTGCCAGG GCTTCCTGGC 
CCGAAAGGAG AGCCTGGTGG AATTACTTTT AAGGGTGAAA GAGGTCCCCC TGGGAACCCA 
GGTTTACCAG GCCTCCCAGG GAATATAGGG CCTATGGGTC CTCCTGGTTT TGGCCCTCCA 
GGCCCAGTAG GTGAAAAAGG CATACAAGGT GTGGCAGGAA ATCCAGGCCA GCCAGGAATA 
CCAGGTCCTA AAGGGGATCC AGGTCAGACT ATAACCCAGC CAGGGAAGCC TGGCTTGCCT 
GGTAACCCAG GCAGAGATGG TGAAGTAGGT CTTCCAGGTG ACCCTGGACT CCCAGGGCAA 
CCAGGCTTGC CAGGGATACC TGGTAGCAAA GGAGAACCAG GTATCCCTGG AATTGGGCTT 
CCTGGACCAC CTGGTCCCAA AGGCTTTCCT GGAATTCCAG GACCTCCAGG AGCACCTGGG 
ACACCTGGAA GAATTGGTCT AGAAGGCCCT CCTGGGCCAC CTGGCTTTCC AGGACCAAAG 
GGTGAACCAG GATTTGCATT ACCTGGGCCA CCTGGGCCAC CAGGACTTCC AGGTTTCAAA 
GGAACGCTTG GTCCAAAAGG TGATCGTGGT TTCCCAGGAC CTCCAGGTCC TCCAGGACGC 
ACTGGTGTAG ATGGGCTCCC TGGACCAAAA GGTGATGTTG GACCAAATGG ACAACCTGGA 
CCAATGGGAC CTCCTGGGCT GCCAGGAATA GGTGTTCAGG GACCACCAGG ACCACCAGGG 
ATTCCTGGGC CAATAGGTCA ACCTGGTTTA CATGGAATAC CAGGAGAGAA GGGGGATCCA 
GGACCTCCTG GACTTGATGT TCCAGGACCC CCAGGTGAAA GAGGCAGTCC AGGGATCCCT 
GGAGCACCTG GTTCTATAGG ACCTCCAGGA TCACCAGGGC TTCCAGGAAA AGCAGGTGCC 
TCTGGATTTC CAGGTACCAA AGGTGAAATG GGTATGATGG GACCTCCAGG CCCACCAGGA 
CCTTTGGGAA TTCCTGGCAG GAGTGGTGTA CCTGGTCTTA AAGGTGATGA TGGCTTGCAG 
GGTCAGCCAG GACTTCCTGG CCCTGCAGGA GAAAAAGGTA GTAAAGGAGA GCCTGGCCTT 
CCAGGCCCTC CTGGACCAAT GGATCCAAAT CTTCTGGGCT CAAAAGGAGA GAAGGGGGAA 
CCTGGCTTAC CAGGTATACC TGGAGTTTCA GGGCCAAAAG GTTATCAGGG CTTGCCTGGA 
GACCCAGGGC AACCTGGACT GAGTGGACAA CCTGGATTAC CAGGACCACC AGGTCCCAAA 
GGTAACCCTG GTCTCCCTGG ACAGCCAGGT CTTATAGGAC CTCCTGGACT TAAAGGAACC 
ATCGGCGATA TGGGTTTTCC AGGGCCTCAG GGTGTGGAAG GGCCTCCTGG ACCTCCTGGA 
GTTCCTGGAC AGCCTGGCTC CCCAGGATTA CCTGGACAGA AAGGAGACAA AGGTGATCCT 
GGTATTTCAA GCATTGGTCT TCCAGGTCTT CCTGGTCCAA AGGGTGAGCC TGGTCTGCCT 
GGATACCCAG GGAACCCTGG TATCAAAGGT TCTGTGGGAG ATCCTGGTTT GCCTGGATTA 
CCAGGAACCC CTGGAGCAAA AGGACAACCA GGCCTTCCTG GATTCCCAGG AACCCCAGGC 
CCTCCTGGAC CAAAAGGTAT TAGTGGCCCT CCTGGGAACC CTGGCCTTCC AGGAGAAACT 
GGTCCTGTAG GTGGTGGAGG TCGTCCTGGG CAACCAGGGC CTCCAGGCGA AAAAGGCAAA 
CCCGGTCAAG ATGGTATTCC TGGACCAGCT GGACAGAAGG GTGAACCAGG TCAACCAGGC 
TTTGGAAACC CAGGACCCCC TGGACTTCCA GGACTTTCTG GCCAAAAGGG TGATGGAGGA 
TTACCTGGAA TTCCAGGAAA TCCTGGCCTT CCAGGTCCAA AGGGCGAACC AGGCTTTCAT 
GGTTTCCCTG GTGTGCAGGG TCCCCCAGGC CCTCCTGGTT CTCCGGGTCC AGCTCTGGAA 
GGACCTAAAG GCAACCCCGG GCCCCAAGGT CCTCCTGGGA GACCAGGTCC TACAGGTTTT 
CAAGGTCTAC CAGGTCCAGA AGGTCCCCCA GGTCTCCCTG GAAATGGAGG TATTAAAGGA 
GAGAAGGGAA ACCCAGGCCA ACCTGGGCTA CCTGGCTTGC CTGGTTTGAA AGGAGATCAA 
GGACCACCAG GACTCCAGGG TAATCCTGGT CGGCCGGGTC TCAATGGAAT GAAAGGAGAT 
CCTGGTCTCC CTGGTGTTCC AGGATTCCCA GGCATGAAAG GACCCAGTGG AATACCTGGA 
TCAGCTGGTC CTGAGGGGGA ACCGGGACTT ACTGGTCCTC CAGGTCCTCC TGGATTACCT 
GGTCCCTCAG GACAGAGTAT CATAATCAAA GGAGATGCTG GTCCTCCAGG AATCCCTGGA 
CAACCTGGGT TAAAAGGTCT ACCAGGACCC CAAGGACCTC AGGGTTTACC AGGTCCAACT 
GGCCCTCCAG GAGATCCTGG ACGCAATGGA CTCCCTGGCT TTGATGGTGC AGGAGGGCGC 
AAAGGAGACC CAGGTCTGCC AGGACAGCCA GGCACCCGTG GTTTGGATGG TCCCCCCGGG 
CCAGATGGAT TGCAAGGTCC CCCAGGTCCC CCTGGAACCT CCTCTATTGC ACATGGATTT 
CTTATTACAC GCCACAGCCA GACAACGGAT GCACCACAAT GCCCGCAGGG AACACTTCAG 
ATCTATGAAG GCTTTTCTCT CCTGTATGTA CAAGGAAATA AAAGAGCCCA TGGCCAAGAC 
TTGGGGACGG CTGGCAGCTG CCTTCGTCGC TTTAGTACCA TGCCTTTTAT GTTCTGCAAC 
ATCAATAATG TTTGCAACTT CGCTTCAAGA AATGACTATT CTTACTGGCT CTCTACCCCA 
GAGCCCATGC CAGTGAGCAT GCAACCCCTA AAGGGCCAGA GCATCCAGCC ATTCATTAGT 
CGATGTGCAG TATGTGAAGC TCCAGCTGTG GTGATCGCAG TTCACAGTCA GACGATCCAG 
ATTCCCCGTT GTCCTCAGGG ATGGGATTCT CTGTGGATTG GTTATTCCTT CATGATGCAT 
ACAAGTGCAG GGGCAGAAGG CTCAGGTCAA GCCCTAGCCT CTCCTGGTTC CTGCTTGGAA 
GAGTTTCGTT CAGCTCCCTT CATCGAATGT CATGGGAGGG GTACCTGTAA CTACTATGCC 
AACTCCTACA GCTTTTGGCT GGCAACTGTA GATGTGTCAG ACATGTTCAG TAAACCTCAG 
TCAGAAACAC TGAAAGCAGG AGACTTGAGG ACACGAATTA GCCGATGTCA AGTGTGCATG 
AAGAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007990723.1
CDS	303..5054
Translation	MPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGVQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVVGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPPGPHIPPSDEICEAGPPGPPGSPGDKGLQGEQGMKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGSKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGSPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGTLGPKGDRGFPGPPGPPGRTGVDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGSIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPAGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPPGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGETGPVGGGGRPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGIPGSAGPEGEPGLTGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSIAHGFLITRHSQTTDAPQCPQGTLQIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPVSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPRCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_007992532.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus collagen, type IV, alpha 5 (COL4A5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007992532.1

ORF Insert Sequence:

ATGCCAGGCC ATGATGGGGC CCCAGGACCT CAAGGTATTC CTGGATGCAA TGGAACCAAG 
GGAGAACGTG GATTTCCAGG CAGTCCCGGT TTTCCTGGTT TACAGGGTCC TCCAGGACCC 
CCTGGGATCC CAGGTATGAA GGGTGAACCA GGTAGTATAA TTATGTCATC ACTGCCAGGA 
CCAAAGGGTA ATCCAGGATA TCCAGGTCCT CCTGGAGTAC AAGGCCTACC TGGTCCCACT 
GGTATACCAG GGCCAATTGG TCCCCCAGGA CCACCAGGTT TGATGGGCCC TCCTGGTCCA 
CCAGGACTTC CAGGACCAAA GGGGAATATG GGCTTAAATT TCCAGGGACC CAAAGGTGAA 
AAGGGTGAGC AAGGTCTTCA GGGCCCACCT GGGCCACCTG GGCAGATCAG TGAACAGAAA 
AGACCAATTG ATGTAGAGTT TCAGAAAGGA GACCAGGGAC TTCCTGGTGA CCGAGGGCCT 
CCTGGACCTC CAGGGATACG TGGTCCTCCA GGTCCCCCAG GTGGTGAGAA AGGTGAGAAG 
GGTGAGCAAG GAGAGCCAGG CAAAAGAGGT AAACCAGGCA AAGATGGAGA AAACGGCCAA 
CCAGGAATTC CTGGTTTGCC TGGTGATCCT GGTTACCCTG GTGAACCCGG AAGGGATGGT 
GAAAAGGGCC AAAAAGGTGA CATTGGCCCA CCTGGACCTC CTGGACTTGT AATTCCTAGA 
CCTGGGACTG GTATAACTAT AGGAGAAAAA GGAAACATTG GGTTGCCTGG CTTGCCCGGA 
GAAAAAGGAG AGCGAGGATT TCCTGGAATA CAGGGCCCAC CTGGCCTTCC TGGACCTCCA 
GGGGCTGCAG TTGTGGGTCC TCCTGGCCCT CCTGGATTTC CTGGAGAAAG GGGTCAGAAA 
GGTGATGAAG GACCACCTGG AATTTCCATT CCTGGACCTC CTGGACTTGA TGGACAGCCT 
GGGGCTCCTG GGCTTCCGGG GCCTCCTGGC CCTCCTGGAC CTCACATCCC TCCTAGTGAT 
GAGATATGTG AAGCAGGCCC TCCAGGCCCC CCAGGATCTC CAGGTGATAA AGGACTCCAA 
GGAGAACAAG GAATGAAAGG TGACAAAGGT GACACTTGCT TCAACTGCAT TGGAACTGGT 
ATTTCAGGGC CTCCAGGTCA ACCTGGTTTG CCAGGTCTCC CAGGTCCTCC AGGATCCCTT 
GGTTTCCCTG GACAGAAAGG TGAAAAAGGA CAAGCTGGTG CAACTGGTTC CAAAGGATTA 
CCAGGCATTC CAGGAGCTCC AGGTGCTCCA GGCTTTCCTG GATCTAAAGG TGAACCTGGT 
GATATCCTCA CTTTTCCAGG AATGAAGGGT GACAAAGGAG AGTTGGGTTC CCCTGGAGCT 
CCAGGGCTTC CTGGTTTACC TGGCTCTCCT GGACAGGATG GATTGCCAGG GCTTCCTGGC 
CCGAAAGGAG AGCCTGGTGG AATTACTTTT AAGGGTGAAA GAGGTCCCCC TGGGAACCCA 
GGTTTACCAG GCCTCCCAGG GAATATAGGG CCTATGGGTC CTCCTGGTTT TGGCCCTCCA 
GGCCCAGTAG GTGAAAAAGG CATACAAGGT GTGGCAGGAA ATCCAGGCCA GCCAGGAATA 
CCAGGTCCTA AAGGGGATCC AGGTCAGACT ATAACCCAGC CAGGGAAGCC TGGCTTGCCT 
GGTAACCCAG GCAGAGATGG TGAAGTAGGT CTTCCAGGTG ACCCTGGACT CCCAGGGCAA 
CCAGGCTTGC CAGGGATACC TGGTAGCAAA GGAGAACCAG GTATCCCTGG AATTGGGCTT 
CCTGGACCAC CTGGTCCCAA AGGCTTTCCT GGAATTCCAG GACCTCCAGG AGCACCTGGG 
ACACCTGGAA GAATTGGTCT AGAAGGCCCT CCTGGGCCAC CTGGCTTTCC AGGACCAAAG 
GGTGAACCAG GATTTGCATT ACCTGGGCCA CCTGGGCCAC CAGGACTTCC AGGTTTCAAA 
GGAACGCTTG GTCCAAAAGG TGATCGTGGT TTCCCAGGAC CTCCAGGTCC TCCAGGACGC 
ACTGGTGTAG ATGGGCTCCC TGGACCAAAA GGTGATGTTG GACCAAATGG ACAACCTGGA 
CCAATGGGAC CTCCTGGGCT GCCAGGAATA GGTGTTCAGG GACCACCAGG ACCACCAGGG 
ATTCCTGGGC CAATAGGTCA ACCTGGTTTA CATGGAATAC CAGGAGAGAA GGGGGATCCA 
GGACCTCCTG GACTTGATGT TCCAGGACCC CCAGGTGAAA GAGGCAGTCC AGGGATCCCT 
GGAGCACCTG GTTCTATAGG ACCTCCAGGA TCACCAGGGC TTCCAGGAAA AGCAGGTGCC 
TCTGGATTTC CAGGTACCAA AGGTGAAATG GGTATGATGG GACCTCCAGG CCCACCAGGA 
CCTTTGGGAA TTCCTGGCAG GAGTGGTGTA CCTGGTCTTA AAGGTGATGA TGGCTTGCAG 
GGTCAGCCAG GACTTCCTGG CCCTGCAGGA GAAAAAGGTA GTAAAGGAGA GCCTGGCCTT 
CCAGGCCCTC CTGGACCAAT GGATCCAAAT CTTCTGGGCT CAAAAGGAGA GAAGGGGGAA 
CCTGGCTTAC CAGGTATACC TGGAGTTTCA GGGCCAAAAG GTTATCAGGG CTTGCCTGGA 
GACCCAGGGC AACCTGGACT GAGTGGACAA CCTGGATTAC CAGGACCACC AGGTCCCAAA 
GGTAACCCTG GTCTCCCTGG ACAGCCAGGT CTTATAGGAC CTCCTGGACT TAAAGGAACC 
ATCGGCGATA TGGGTTTTCC AGGGCCTCAG GGTGTGGAAG GGCCTCCTGG ACCTCCTGGA 
GTTCCTGGAC AGCCTGGCTC CCCAGGATTA CCTGGACAGA AAGGAGACAA AGGTGATCCT 
GGTATTTCAA GCATTGGTCT TCCAGGTCTT CCTGGTCCAA AGGGTGAGCC TGGTCTGCCT 
GGATACCCAG GGAACCCTGG TATCAAAGGT TCTGTGGGAG ATCCTGGTTT GCCTGGATTA 
CCAGGAACCC CTGGAGCAAA AGGACAACCA GGCCTTCCTG GATTCCCAGG AACCCCAGGC 
CCTCCTGGAC CAAAAGGTAT TAGTGGCCCT CCTGGGAACC CTGGCCTTCC AGGAGAAACT 
GGTCCTGTAG GTGGTGGAGG TCGTCCTGGG CAACCAGGGC CTCCAGGCGA AAAAGGCAAA 
CCCGGTCAAG ATGGTATTCC TGGACCAGCT GGACAGAAGG GTGAACCAGG TCAACCAGGC 
TTTGGAAACC CAGGACCCCC TGGACTTCCA GGACTTTCTG GCCAAAAGGG TGATGGAGGA 
TTACCTGGAA TTCCAGGAAA TCCTGGCCTT CCAGGTCCAA AGGGCGAACC AGGCTTTCAT 
GGTTTCCCTG GTGTGCAGGG TCCCCCAGGC CCTCCTGGTT CTCCGGGTCC AGCTCTGGAA 
GGACCTAAAG GCAACCCCGG GCCCCAAGGT CCTCCTGGGA GACCAGGTCC TACAGGTTTT 
CAAGGTCTAC CAGGTCCAGA AGGTCCCCCA GGTCTCCCTG GAAATGGAGG TATTAAAGGA 
GAGAAGGGAA ACCCAGGCCA ACCTGGGCTA CCTGGCTTGC CTGGTTTGAA AGGAGATCAA 
GGACCACCAG GACTCCAGGG TAATCCTGGT CGGCCGGGTC TCAATGGAAT GAAAGGAGAT 
CCTGGTCTCC CTGGTGTTCC AGGATTCCCA GGCATGAAAG GACCCAGTGG AATACCTGGA 
TCAGCTGGTC CTGAGGGGGA ACCGGGACTT ACTGGTCCTC CAGGTCCTCC TGGATTACCT 
GGTCCCTCAG GACAGAGTAT CATAATCAAA GGAGATGCTG GTCCTCCAGG AATCCCTGGA 
CAACCTGGGT TAAAAGGTCT ACCAGGACCC CAAGGACCTC AGGGTTTACC AGGTCCAACT 
GGCCCTCCAG GAGATCCTGG ACGCAATGGA CTCCCTGGCT TTGATGGTGC AGGAGGGCGC 
AAAGGAGACC CAGGTCTGCC AGGACAGCCA GGCACCCGTG GTTTGGATGG TCCCCCCGGG 
CCAGATGGAT TGCAAGGTCC CCCAGGTCCC CCTGGAACCT CCTCTATTGC ACATGGATTT 
CTTATTACAC GCCACAGCCA GACAACGGAT GCACCACAAT GCCCGCAGGG AACACTTCAG 
ATCTATGAAG GCTTTTCTCT CCTGTATGTA CAAGGAAATA AAAGAGCCCA TGGCCAAGAC 
TTGGGGACGG CTGGCAGCTG CCTTCGTCGC TTTAGTACCA TGCCTTTTAT GTTCTGCAAC 
ATCAATAATG TTTGCAACTT CGCTTCAAGA AATGACTATT CTTACTGGCT CTCTACCCCA 
GAGCCCATGC CAGTGAGCAT GCAACCCCTA AAGGGCCAGA GCATCCAGCC ATTCATTAGT 
CGATGTGCAG TATGTGAAGC TCCAGCTGTG GTGATCGCAG TTCACAGTCA GACGATCCAG 
ATTCCCCGTT GTCCTCAGGG ATGGGATTCT CTGTGGATTG GTTATTCCTT CATGATGCAT 
ACAAGTGCAG GGGCAGAAGG CTCAGGTCAA GCCCTAGCCT CTCCTGGTTC CTGCTTGGAA 
GAGTTTCGTT CAGCTCCCTT CATCGAATGT CATGGGAGGG GTACCTGTAA CTACTATGCC 
AACTCCTACA GCTTTTGGCT GGCAACTGTA GATGTGTCAG ACATGTTCAG TAAACCTCAG 
TCAGAAACAC TGAAAGCAGG AGACTTGAGG ACACGAATTA GCCGATGTCA AGTGTGCATG 
AAGAGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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COL4A5 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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