ORF » Species Summary » Chlamydomonas reinhardtii » POLA1 cDNA ORF clone

POLA1 cDNA ORF clone, Chlamydomonas reinhardtii

The following POLA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCh05919 XM_001698656.1
Latest version!		 Chlamydomonas reinhardtii DNA polymerase alpha subunit one (POLA1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    POLA1 ( XM_001698656.1 ) cDNA ORF clone, Chlamydomonas reinhardtii -> XP_001698708.1 Chlamydomonas reinhardtii DNA polymerase alpha subunit one (POLA1), partial mRNA.
    Price & Availability↑
    CloneID OCh05919
    Clone ID Related Accession (Same CDS sequence) XM_001698656.1
    Accession Version XM_001698656.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1503849600000
    Organism Chlamydomonas reinhardtii
    Product DNA polymerase alpha subunit one
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843792). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGACCGTA GCAGGCGTCA GCCAGTTCAG AGCAGCGCAG CTGTGAAGGC CAAGGGCGCG 
    CTTGAGCAGC TGCGTGCGGC CCGCGACGGT GGCCTCAAGC GTGCACTTGC GTATGAGGTC 
    AAGGAGGAGG CAGCTGTCTA TGATGTGGTC GACGAGCAGC AATATGCTGA AATTGTGAAG 
    AAGCGCCGCG ACGCCGGGGA TTTCGTTGTG GATGACGATG GGGCTGGTTA CCATGACATC 
    GGCGAGGATG ACTACTTCCA GGAGCGCCCC GTCGAGGACG ACGGCGGCGA CGATGGGGAG 
    GACGACACTA AGCAGGCGGG CAAGAAGCGC AAGAAGGGCG TGCTGCCGTT CTATCTGCTT 
    GATGCATACG AGAACCCGGA CGCGCGTCCC GGCGAGGTGG TGCTGTTTGG CAAGATCGAG 
    GCGGAGGGCG GCCGCTGGCA GAGCTGCGCC GTGGCGGTGC GCGGGCTGCA GCGCACACTG 
    ATTGTGGTGC CGCGGCGGGA GGTGTTTGAG GACGGCGACG GCGCGATCGT GGCCATGCAC 
    GCCGCCGTCA AGGCCGACCC CGCCCGCAAG CCCGAGCTGA TGAAGATGCT GCAGATGTGG 
    TCGGCAGCTG GTGGAGTGTT TTTAGAGGGC ATCAAGTTCT ACGCCTTTGA GAACAGGGAC 
    GTGCCGCACG GAGAGCAGTG GGTCATCAAG GTGCGCTACC CCGGCCACCT GCCAGTGCTT 
    CAGCCGCCCG CCTCGGGCTG CTTCAGCGGC AAGACCTACT GCGCCATCTT CGGAGCCAAC 
    CAGAGCACCC TGGAGCGGCG CATCAAGGGC CCGGGCTGGA TGGGCATCTG CAGCCCCACC 
    CGCGTGGAGT ACGGCAACCA GACCACCTGG TGCAAGGTGG AGGTGGCGCA CGAGATCGTG 
    GCTGCCAGTG TGGTGCACCT GTCGGGCGTG TCGCCCGACG CGCCCCTGGC TGCCAAGGAC 
    TGGAGCAACC CGCAACGCCT GCGCAGCTTC TCCATAGTGC GACGGCTGGA TGGACAGGCC 
    TGGCCCGCGG GCATGGAGGC GGCCGCCGCC GCCGAGAACG CCACGCCGCG CGGCCGCGCC 
    AACAGCGGCG CCGGCAACGC CAACGGGCCG CTGGTGGCGC TGCAGGGCTC GGAGCGCTCG 
    CTGCTCACAT GCCTGCTGGC CAGGCTGCAG TCGCTGGACG CGGACGTGCT GGTGGGACAC 
    AACGTGGCGG CCTTTGACCT CACCACCCTG CTGTCGCGCA TGCAGCACCA CAAGGTGCCG 
    CTGTGGAGCC GCATCGGCCG CGTGAAGAAG ACGGAGTTCC CGCGCCTCAC CGGGGGCGGC 
    CACACCTTTG GCGGCGGCGC GGGTGCCGGC GTCATGGCCA CTGTGGCGGG CCGGCTGATG 
    TGCGACACCT ACCTGTCGGC TCGTGAGCTG GTCAAGAGCG TGGACTTCAC ACTGGGAACG 
    CTGGCGCAGT CGCTGCTGGG TCAGCAGCGC TCCGACCCGC TGGCCGGCTC CAACGCGGGC 
    AACAACAGCG GGCTGGGCGC GTGCTTTGGC AGTGCGGAGG GCGTGCTGCG CCTGCTGCGC 
    CACGGCGAGA GCGACGCATG GCTGGCGCTG GGCATCATGT TCCACCTGAG CGTGCTGCCG 
    CTCACCAAGC AGCTGTCTGC GCTGTCGGGC CAGCTGTGGA GCCGCACGCT GGCGGGGCAG 
    CGCGCCGGCC GCATCGAGAT GCTGCTGCTG CACGAGTTCC ACAGCCGCAA GTTCATCGTG 
    CCGGACAAGC AGAGCTACAA GTCCAAGGCC GCCCCCTCCG GCAACGGCCC CAAGTACGCG 
    GGCGGTCTGG TGCTCGAGCC CAAGAAGGGG CTGTACGACA AGATTGTCAT CATCCTGGAC 
    TTCAACTCCC TGTACCCCTC CATCATCCAG GAGTACAACA TCTGCTTCAC CACGGCCATC 
    AAGCTGACCG CCAACTCCAT GTACGGCTGC CTGGGCTTTG GCGCATCGCG CTTCTACGCA 
    CAGCCGCTGG CCGAGCTCAT CACCGCGCAG GGCCGCTCCA TCCTGAGCAG CACGGTGGAC 
    CTGGAGGCGC GGGCGCTGGG CGCCCGCATC AAGCGCGAGG TCAACAAGCG CTACAAGCTG 
    CTGGAGATCG AGCTGGACGG CGTGTTTAAA TCCATCTTGC TGCTCAAGAA GAAAAAGTAC 
    GCGGCCGTGA AGCTGGAGCC GGACGGCCAG GGCAGGCTGG CGGAGGTGAT GGAGCAGAAG 
    GGCCTGGACA TTGTGCGGCG CGACTGGTGC CCGCTGTCCA AGGACGTCGG CAACTTCGCG 
    CTGGCAGCCG TGCTGAGCGG CCGCGGGCGG GAGGAGGTGG TGGGCGAGAT CCACGCACAC 
    CTGCGCCAGG TGGCGGAGCG GGTGCGCGCA GGACAGATCC CCATGGGCAA GTTCATCATC 
    ACCAAGCAGC TCACCAAGCG GCCCGAGGAC TACCCAGACG CCAAGAACCA GCCGCACGTG 
    CAGGTGGCCA TGAGGCGGCG GGCGGCGGGC AAGCGCGACG GCGTGCTGCC TATCCACCCT 
    GTGGTGTCGC GCCTAGTTAG CCCTATCGAG GGCACCGACG CCGCCCACAT CGCCGACTGC 
    CTGGGTCTGG ACCCCGCCCG CGGCGGCACC CTCATGGACG AGGACGAGAA CTTCAGGTCG 
    TGTGGCCCGC TGCTGCTGAC CAGCCCCTCC GGCGCCTGCT TCCCGCTGCG GGGTGTGGAC 
    GACGTGCTCA AGGAAGCAAT CATGCCAGAG CAGCTGCTGA CGCCGCCGGA CGCGCTGGGC 
    GACCCCACCG CCACTGTCAG CCCTGCGGCA CTCATCAACC AGGTGCTGCT GCGCGCGCGG 
    GAGGCGGTGG CGCGCTACTA CGACGGCGTG CTCCGACCCG ACGACGAGAC GGCGGACGCG 
    GGGCCAGGCG GCTGCCGCGC TGCACTGTCG CACTACTACC GGCTACTGCA CGTGGAGGGC 
    GCGGCGCGGC GCCACCACTG CCGCCTGCGC GCCGCCGACC CCAAGAGCGG CGTCAGCCTA 
    GACGACGTGC GGGCCAAGGT CCCGGAGTCG CTGGCGCGCA CGCTGGGCGC CGCCGCCGAG 
    GCGCTGGACG GCCTGCGCCG CCGCAGCCAT TGGCACTGGC GGATGCTGTA TGTGGTTGCT 
    GCACATTTGC GCCAGGGTTG GGGAGTGCTG CGCGCACTTC AGTTGGAAGG GACTCGCGCC 
    TGGATGCGCT GCGCCTGCTG CGTCATCTGC ATCCTGTACT CCTGCGACGG CACTACTGAC 
    GTCGGCGACC TGCGGCGGCG GCCGCCATGC CAGCCCTGGG GCCGGCAGCA GCGGCGCTGC 
    CTGCAGTGCC GCCGCGTGTT CGCCGCCCCG GCCACAGCTA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001698708.1
    CDS1..3345
    Translation

    Target ORF information:

    RefSeq Version XM_001698656.1
    Organism Chlamydomonas reinhardtii
    Definition Chlamydomonas reinhardtii DNA polymerase alpha subunit one (POLA1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001698656.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGACCGTA GCAGGCGTCA GCCAGTTCAG AGCAGCGCAG CTGTGAAGGC CAAGGGCGCG 
    CTTGAGCAGC TGCGTGCGGC CCGCGACGGT GGCCTCAAGC GTGCACTTGC GTATGAGGTC 
    AAGGAGGAGG CAGCTGTCTA TGATGTGGTC GACGAGCAGC AATATGCTGA AATTGTGAAG 
    AAGCGCCGCG ACGCCGGGGA TTTCGTTGTG GATGACGATG GGGCTGGTTA CCATGACATC 
    GGCGAGGATG ACTACTTCCA GGAGCGCCCC GTCGAGGACG ACGGCGGCGA CGATGGGGAG 
    GACGACACTA AGCAGGCGGG CAAGAAGCGC AAGAAGGGCG TGCTGCCGTT CTATCTGCTT 
    GATGCATACG AGAACCCGGA CGCGCGTCCC GGCGAGGTGG TGCTGTTTGG CAAGATCGAG 
    GCGGAGGGCG GCCGCTGGCA GAGCTGCGCC GTGGCGGTGC GCGGGCTGCA GCGCACACTG 
    ATTGTGGTGC CGCGGCGGGA GGTGTTTGAG GACGGCGACG GCGCGATCGT GGCCATGCAC 
    GCCGCCGTCA AGGCCGACCC CGCCCGCAAG CCCGAGCTGA TGAAGATGCT GCAGATGTGG 
    TCGGCAGCTG GTGGAGTGTT TTTAGAGGGC ATCAAGTTCT ACGCCTTTGA GAACAGGGAC 
    GTGCCGCACG GAGAGCAGTG GGTCATCAAG GTGCGCTACC CCGGCCACCT GCCAGTGCTT 
    CAGCCGCCCG CCTCGGGCTG CTTCAGCGGC AAGACCTACT GCGCCATCTT CGGAGCCAAC 
    CAGAGCACCC TGGAGCGGCG CATCAAGGGC CCGGGCTGGA TGGGCATCTG CAGCCCCACC 
    CGCGTGGAGT ACGGCAACCA GACCACCTGG TGCAAGGTGG AGGTGGCGCA CGAGATCGTG 
    GCTGCCAGTG TGGTGCACCT GTCGGGCGTG TCGCCCGACG CGCCCCTGGC TGCCAAGGAC 
    TGGAGCAACC CGCAACGCCT GCGCAGCTTC TCCATAGTGC GACGGCTGGA TGGACAGGCC 
    TGGCCCGCGG GCATGGAGGC GGCCGCCGCC GCCGAGAACG CCACGCCGCG CGGCCGCGCC 
    AACAGCGGCG CCGGCAACGC CAACGGGCCG CTGGTGGCGC TGCAGGGCTC GGAGCGCTCG 
    CTGCTCACAT GCCTGCTGGC CAGGCTGCAG TCGCTGGACG CGGACGTGCT GGTGGGACAC 
    AACGTGGCGG CCTTTGACCT CACCACCCTG CTGTCGCGCA TGCAGCACCA CAAGGTGCCG 
    CTGTGGAGCC GCATCGGCCG CGTGAAGAAG ACGGAGTTCC CGCGCCTCAC CGGGGGCGGC 
    CACACCTTTG GCGGCGGCGC GGGTGCCGGC GTCATGGCCA CTGTGGCGGG CCGGCTGATG 
    TGCGACACCT ACCTGTCGGC TCGTGAGCTG GTCAAGAGCG TGGACTTCAC ACTGGGAACG 
    CTGGCGCAGT CGCTGCTGGG TCAGCAGCGC TCCGACCCGC TGGCCGGCTC CAACGCGGGC 
    AACAACAGCG GGCTGGGCGC GTGCTTTGGC AGTGCGGAGG GCGTGCTGCG CCTGCTGCGC 
    CACGGCGAGA GCGACGCATG GCTGGCGCTG GGCATCATGT TCCACCTGAG CGTGCTGCCG 
    CTCACCAAGC AGCTGTCTGC GCTGTCGGGC CAGCTGTGGA GCCGCACGCT GGCGGGGCAG 
    CGCGCCGGCC GCATCGAGAT GCTGCTGCTG CACGAGTTCC ACAGCCGCAA GTTCATCGTG 
    CCGGACAAGC AGAGCTACAA GTCCAAGGCC GCCCCCTCCG GCAACGGCCC CAAGTACGCG 
    GGCGGTCTGG TGCTCGAGCC CAAGAAGGGG CTGTACGACA AGATTGTCAT CATCCTGGAC 
    TTCAACTCCC TGTACCCCTC CATCATCCAG GAGTACAACA TCTGCTTCAC CACGGCCATC 
    AAGCTGACCG CCAACTCCAT GTACGGCTGC CTGGGCTTTG GCGCATCGCG CTTCTACGCA 
    CAGCCGCTGG CCGAGCTCAT CACCGCGCAG GGCCGCTCCA TCCTGAGCAG CACGGTGGAC 
    CTGGAGGCGC GGGCGCTGGG CGCCCGCATC AAGCGCGAGG TCAACAAGCG CTACAAGCTG 
    CTGGAGATCG AGCTGGACGG CGTGTTTAAA TCCATCTTGC TGCTCAAGAA GAAAAAGTAC 
    GCGGCCGTGA AGCTGGAGCC GGACGGCCAG GGCAGGCTGG CGGAGGTGAT GGAGCAGAAG 
    GGCCTGGACA TTGTGCGGCG CGACTGGTGC CCGCTGTCCA AGGACGTCGG CAACTTCGCG 
    CTGGCAGCCG TGCTGAGCGG CCGCGGGCGG GAGGAGGTGG TGGGCGAGAT CCACGCACAC 
    CTGCGCCAGG TGGCGGAGCG GGTGCGCGCA GGACAGATCC CCATGGGCAA GTTCATCATC 
    ACCAAGCAGC TCACCAAGCG GCCCGAGGAC TACCCAGACG CCAAGAACCA GCCGCACGTG 
    CAGGTGGCCA TGAGGCGGCG GGCGGCGGGC AAGCGCGACG GCGTGCTGCC TATCCACCCT 
    GTGGTGTCGC GCCTAGTTAG CCCTATCGAG GGCACCGACG CCGCCCACAT CGCCGACTGC 
    CTGGGTCTGG ACCCCGCCCG CGGCGGCACC CTCATGGACG AGGACGAGAA CTTCAGGTCG 
    TGTGGCCCGC TGCTGCTGAC CAGCCCCTCC GGCGCCTGCT TCCCGCTGCG GGGTGTGGAC 
    GACGTGCTCA AGGAAGCAAT CATGCCAGAG CAGCTGCTGA CGCCGCCGGA CGCGCTGGGC 
    GACCCCACCG CCACTGTCAG CCCTGCGGCA CTCATCAACC AGGTGCTGCT GCGCGCGCGG 
    GAGGCGGTGG CGCGCTACTA CGACGGCGTG CTCCGACCCG ACGACGAGAC GGCGGACGCG 
    GGGCCAGGCG GCTGCCGCGC TGCACTGTCG CACTACTACC GGCTACTGCA CGTGGAGGGC 
    GCGGCGCGGC GCCACCACTG CCGCCTGCGC GCCGCCGACC CCAAGAGCGG CGTCAGCCTA 
    GACGACGTGC GGGCCAAGGT CCCGGAGTCG CTGGCGCGCA CGCTGGGCGC CGCCGCCGAG 
    GCGCTGGACG GCCTGCGCCG CCGCAGCCAT TGGCACTGGC GGATGCTGTA TGTGGTTGCT 
    GCACATTTGC GCCAGGGTTG GGGAGTGCTG CGCGCACTTC AGTTGGAAGG GACTCGCGCC 
    TGGATGCGCT GCGCCTGCTG CGTCATCTGC ATCCTGTACT CCTGCGACGG CACTACTGAC 
    GTCGGCGACC TGCGGCGGCG GCCGCCATGC CAGCCCTGGG GCCGGCAGCA GCGGCGCTGC 
    CTGCAGTGCC GCCGCGTGTT CGCCGCCCCG GCCACAGCTA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Chlamydomonas genome reveals the evolution of key animal and plant functions.
    Science (New York, N.Y.)318(5848)245-50(2007 Oct)
    Merchant SS,Prochnik SE,Vallon O,Harris EH,Karpowicz SJ,Witman GB,Terry A,Salamov A,Fritz-Laylin LK,Maréchal-Drouard L,Marshall WF,Qu LH,Nelson DR,Sanderfoot AA,Spalding MH,Kapitonov VV,Ren Q,Ferris P,Lindquist E,Shapiro H,Lucas SM,Grimwood J,Schmutz J,Cardol P,Cerutti H,Chanfreau G,Chen CL,Cognat V,Croft MT,Dent R,Dutcher S,Fernández E,Fukuzawa H,González-Ballester D,González-Halphen D,Hallmann A,Hanikenne M,Hippler M,Inwood W,Jabbari K,Kalanon M,Kuras R,Lefebvre PA,Lemaire SD,Lobanov AV,Lohr M,Manuell A,Meier I,Mets L,Mittag M,Mittelmeier T,Moroney JV,Moseley J,Napoli C,Nedelcu AM,Niyogi K,Novoselov SV,Paulsen IT,Pazour G,Purton S,Ral JP,Riaño-Pachón DM,Riekhof W,Rymarquis L,Schroda M,Stern D,Umen J,Willows R,Wilson N,Zimmer SL,Allmer J,Balk J,Bisova K,Chen CJ,Elias M,Gendler K,Hauser C,Lamb MR,Ledford H,Long JC,Minagawa J,Page MD,Pan J,Pootakham W,Roje S,Rose A,Stahlberg E,Terauchi AM,Yang P,Ball S,Bowler C,Dieckmann CL,Gladyshev VN,Green P,Jorgensen R,Mayfield S,Mueller-Roeber B,Rajamani S,Sayre RT,Brokstein P,Dubchak I,Goodstein D,Hornick L,Huang YW,Jhaveri J,Luo Y,Martínez D,Ngau WC,Otillar B,Poliakov A,Porter A,Szajkowski L,Werner G,Zhou K,Grigoriev IV,Rokhsar DS,Grossman AR


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