SFI1 cDNA ORF clone, Chlamydomonas reinhardtii

Gene
Clones
gRNAs

Summary

Gene Symbol	SFI1
Entrez Gene ID	5720767
Full Name	spindle pole body protein
Gene Type	protein-coding
Organism	Chlamydomonas reinhardtii

mRNA and Protein(s)

mRNA	Protein	Name
XM_001695192.1	XP_001695244.1	spindle pole body protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Maréchal-Drouard L, Marshall WF, Qu LH, Nelson DR, Sanderfoot AA, Spalding MH, Kapitonov VV, Ren Q, Ferris P, Lindquist E, Shapiro H, Lucas SM, Grimwood J, Schmutz J, Cardol P, Cerutti H, Chanfreau G, Chen CL, Cognat V, Croft MT, Dent R, Dutcher S, Fernández E, Fukuzawa H, González-Ballester D, González-Halphen D, Hallmann A, Hanikenne M, Hippler M, Inwood W, Jabbari K, Kalanon M, Kuras R, Lefebvre PA, Lemaire SD, Lobanov AV, Lohr M, Manuell A, Meier I, Mets L, Mittag M, Mittelmeier T, Moroney JV, Moseley J, Napoli C, Nedelcu AM, Niyogi K, Novoselov SV, Paulsen IT, Pazour G, Purton S, Ral JP, Riaño-Pachón DM, Riekhof W, Rymarquis L, Schroda M, Stern D, Umen J, Willows R, Wilson N, Zimmer SL, Allmer J, Balk J, Bisova K, Chen CJ, Elias M, Gendler K, Hauser C, Lamb MR, Ledford H, Long JC, Minagawa J, Page MD, Pan J, Pootakham W, Roje S, Rose A, Stahlberg E, Terauchi AM, Yang P, Ball S, Bowler C, Dieckmann CL, Gladyshev VN, Green P, Jorgensen R, Mayfield S, Mueller-Roeber B, Rajamani S, Sayre RT, Brokstein P, Dubchak I, Goodstein D, Hornick L, Huang YW, Jhaveri J, Luo Y, Martínez D, Ngau WC, Otillar B, Poliakov A, Porter A, Szajkowski L, Werner G, Zhou K, Grigoriev IV, Rokhsar DS, Grossman AR
Science (New York, N.Y.)318(5848)245-50(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SFI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SFI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh03715	XM_001695192.1 Latest version!	Chlamydomonas reinhardtii spindle pole body protein (SFI1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SFI1 ( XM_001695192.1 ) cDNA ORF clone, Chlamydomonas reinhardtii -> XP_001695244.1 Chlamydomonas reinhardtii spindle pole body protein (SFI1), partial mRNA.

Price & Availability↑

CloneID	OCh03715
Clone ID Related Accession (Same CDS sequence)	XM_001695192.1
Accession Version	XM_001695192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1503849600000
Organism	Chlamydomonas reinhardtii
Product	spindle pole body protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843697). COMPLETENESS: incomplete on both ends.

ATGACGATCA ACAATCCCCT TAGACTGAGC TCGGAGGTGT CGCCGCTGCG ACATGTGCTG 
GATATCACAG CACGGTCCCA GGGGAAGGAT TTGACTGCAT ATGAGACCTA TCGGGCTCTC 
CCCAGCCCGA CGCGGCCATA CAGCGGCTAC GACTACGCCG TGGTCGCCGA AATCATCCGG 
GCTGCGGAGG TCACGGTCGA CAAGGCGCAC AAGAAGGGGC TCGGCTCAGG CATCGTCACG 
CTGCAGTCAG TGCTGCAGGC ATACGAGCAC GTGCTGCCGC GCCATGGTGT GAAGGCGGAT 
GAGGACACGT ACTACTACCG CCTGCTGCTC AAGCTCAGCC TAGACCCAGC GCTGGACTGG 
TGGATCAAGC TAAACCGGGA GACTGGGACT ACGGGCGGTC GCGCCGCGTT CTTCAGCAAT 
GCCGGCAGCG ACGTGACGTC CATGGGGCGG TCGCACAGCT TCTACCGCGG CGACACCTCG 
CCCACACGGG TCCAGCCCCG CACCTCGGGT GCCGGCGCTA GTGACTATTC GGCCAGCCAC 
AGCGCTGCGC CCACCTTCCG CAGCAGCATA CCGGGCAACG GCGCAGCGGA CGGGGGAAGT 
CGGGTGCCCG GCCGCAACTC CCCCTACCGG TCCACGGCCT CTCTCTCCAG CATGCCCGGC 
TACCAGCAGC AGCAAGAGAC CGCCGCACAG GCAGCTGCGC GGGAGGCGGC GGCGCGCGCG 
AATGCTGCGG CGCAAGCCGC ACGGGATGCG ATTGAAGCGG CGCGGCAGGC GGCCGCCACG 
GCGCACCTAC CATCTTCGCG CTTCATGTTC AACACACCCC CTATGACGCC GGCTATGGGA 
CCTGTACCGC CACCGTCAGC TCGGACCGAA GGTGGCGCCA GCGGCTGGGG GGATGGCAGT 
ATGGGGGGCG CGGGCAGCAT CGTGCCGTAC AGCGTGTCGG AGGGCGGCGC CACGGGCCCG 
GGAGGGGCTT CCCAATATGG ACCGGGAAGC AGATACGGGC CAGGGAGCCC CCATGGCACG 
ACTGCGCCTA GCAGCGTGCG GGGCACCTAC GGCATCAGCG AGCACGACCC CCTCAGTCCT 
GATGCGTCGG CGTATGGGGC TGGATCTCGG GGCACCCGGA GTGGCGGCGG AGCCGATGGT 
GGCAGTATGG GTGGCGCAGT AGGGCCGGGC GCCACCCGTG CCAGTAGCGG AGGCGGCGGT 
GGTCCCGGCC GGGTCAGCGC CGCCCAGCTG GCGGAGCTGG ACGAGCCGGT GCTGAGCGAG 
GAGGCGGAGG CTTACCTGGA CTACAATCGC CTGGCGCGCG CCTTCAGGAT GTGGCGCAAG 
AACACCTTTG CCCGCAAGGC GTCCAGGTTT GAGCGCGACC ATGCGCTCAA AAATTGGGCT 
GTGGCGACCT CGTTCTGGGC GGTGCACCTG CTGCGCCGCT GCTTCGCGCG CTGGCGTGCA 
GAAGGGCCGA GCAAGACGGC GCTGGCGCTG CAGCTTTGGG CGGGCCACAG CCTGTCAGCC 
TGCTTCTGGC GGTGGCTGAG CCTCACGCGC TACCTCCGGG GAAAAGGGAC CGAATGCGAT 
GGGCACTGGC GCGTGCGGAC GCTGCGCCGC TGCCTGCGCA TGTGGCGTCT CTACACTCGG 
TCCCAGCAGC TCAAGGCCGA CAACGTGGAG AAGGCACTGG GCTTTTGGGT TGGCCGTTCC 
CTGCACATCT GCTTCCGCGT GTGGCGCCAG TTTGCGGCAA ACCAGCTGCG CAAGAATGAG 
GCGGTGCAGA AGGCGGTCGG GCACTGGGCG GGCAAGTCGC TGCGGGCCTG CTTTGAGGTC 
TGGCGCGAGT TCACAATGCT TCAGCAGCAC AAGTCGGTGG CGACCGCCCG GGCGCTGCGC 
CACTGGACCG GCACCACGCT GCGCAGCTGC TTCGCGCTGT GGCGGCTGCT TGTCGAGGAC 
GCGCACAGCA ACTGGGACCA GGCCACACAG CACTTGATGC GACGGATGCT GCTCAAGTGG 
CGCATTGCGG CCAAGCAGCT AACCCGCATG AGCGCTGCCT ACCACGCCAT CGCGGACGCT 
ACTGCGCGTC GGGTCATGTC GCAGGCCATG CGGACGCTGC TGTCGGCGCA CGCCTTCCGG 
CACGAGACCG CTAACCGGCT CCGTGCGGCG GTGGCGCGCA TGCGCAACTC GCTTCTGTCC 
GCCGCCTTCA ACAAGTGGAG CGAGGCGCGC CAAGCCGGCC AGATGAAGCG GGAGCGGATG 
GCGTTGGCGC TCAAGTGGTG GTCGTCCAGA GGCCTGGCTC GCAGCTGGAA CACCTGGCGC 
GAGGCGGTGG CTGCAAGCCA GCGCGCGACG AAGGCGGCGC GGGCGAATGC GCAGCGCATC 
CTGCGGCCGC TGCTACGTGA CACCTTCTAC GCCTGGCTGG ACTGGCACGA CGTCAGGTTG 
CAGAAAGAGG TCCGCGCGGT AGCGGCGCGG CGAACCATGC AGCTGCGGCA GATGCGGCTG 
GTGGTGCTGG GCTGGCGGGG GCTGGTGCTG GAGCGGCGGC ACAGCCTGGT GTCGTTCCAG 
CGCATCATGC GGAATGAGGT GCAGCGGATT ATGAGCACAG CCTTCCAGCG ATGGTACCGC 
GAGCATGACA ACCTGCGACG TGCGGCAGAG GCGCGGCTGA CGGTGAAGCG AAACGCGACC 
AAACGCTGCC TGCGCCACTG GCGGGCGCTA GCGCAGGCAC AGCGCGCGCA CAGGGAGTGG 
CGGCTGGAGC GGCTGCGGCT AGCGAGCGAG CAGCGGCAAC GCCGGCTGCT GGTGGCGTGG 
CAGGTGGTCG CGGGGCTGCT GGCGGAGCAC AACCGGCTAG TCAAGTCCAG TTTGTTCAAG 
CTGCACCGGC GGCAGGCGCA CTCTGTGCTG GCGGCCTGGC GCGACCGCGT GCTGCACTCG 
GTGGCCAAGC GCATGAAGCT GCTGGCGGGG CTGCTGTACT GGGAGCAGCA GGTGAAGGGC 
CGCGCCTGGC TGGCCTGGCG GCAGCGCCAG CACGGCTGGC GCATCAAGGC CGCCAAGGGG 
GAGCTGGCGG GCCGGCACTG GCGCACGCGG CGCCTGGCGC TATGCTTCGC GGTGTTTTTG 
GCGCTGTGGC AAGCGTACCA GGGTCGCCTG ACGCGCGCGC TGCTGTTCCG GTCCCGGCGC 
AACGACATCA TCAAGGCGGA TGTGCTGGAC GAATGGCGGC GCCTGGTGGG CTGGCTGCAG 
TGGAAGAACG ACAAGATCCG CAGGGCGCTG GCACACCATC GCTCCAAGCT CGGGGGAGGC 
GCCTTCTACG GCTGGTACGC AGTCACCCGT CACTCCATCG CCACTAAGCG GGCCGTGGCC 
GCGGCTGTGC TCCACTGGGT TCGCCGCCGC CGCGCCGCCG CCTTCGTGTG GTGGCGCAAT 
TGGACCGCCT ACCGCCGCGA CCTGCGCGTG CGCGGTGCGG GCTACGCGCT TGTGCGGGCG 
GCGCGGGCCA AGGAGAAGGT CGTGCTGGCG TTCAAGGCCA ACGTGATCCG CAAGGCTCGC 
ATCGCCGGCG CGGTGGCTCA CCGGCAGCGC TACATGACGC GCCTAGCGCT GTCGGGCTGG 
CTGCTGCGGA CGCTGCTGGC AGCGGATTTC GTGCGGAGGC TGAAGGCTGT GGGCGCGCAG 
CTCAATGTCC AGCTGGTGGT GGACGGGCTG GAGGCCTGGC GCGAGTACAC CATCTACCGC 
CGCACCAAGA AGGGCATGCT GCACGCAGCT CTGCGCTACT GGCGCCTCAG TCGGCAGCGC 
GCGGCCATGG ACGCCATGCG CTGGTACACC ACCCGGCGAC AGCTCAAGCG CGCGGTGCTG 
CTGCGCGGCC GCACTGGCGC CGCGGCAAGG GCGCTGCGGG CTTGGCGCGA CGAGGCGGAG 
TACCGCCGCC GCCTGCGCGA CCGCTACGCG ATGCTGGCCT CCGGCAGCCG GCACGCGGCG 
CTGCGGCGGG GGCTGGACAT GTGGCGCGAC TACATCGCGA ACCGCGCCTC AAAGAAGGGG 
CTGCGGGAGC AGGCGCTGCG GCACTGGCTG CGAGGCACGG CTGGGCGGGT GTTCCGCAGC 
TGGCGGGACT ACCAGCGCCA CCTGCGGCAC GCCGAGGAGC GCGCTGGCGA CATCGTGCAG 
CGTTGGCGGA AGCGGGATGC GGCTGAGGCG CTGGCGGCGT TCGCCGAGTA TGCCGCGCAC 
CGCCGCCGCA AGCGCACCGC GGACGCGATG TCACGTCGCA GCCGCGCCCG CACCGCGCTG 
CAGGCGTGGC GCGAGTACAT GAAGCTGCGC ACGGCGGAAG GCTTCTGGAG CCGCAGCCGC 
CTGAACGATG CCATCGGGGC ATGGCGGCAC TACGCCAACC ACCGCCGTCG GCTGAAAGAG 
CTGTGGGGCC GCACCCGCCG TGGCCGGCTG GCCTCCGCGC TGTCCAGCTG GCGCGACTAC 
GCCACCTACT CGGCCCGCCT CAAGACCGTG TCCAACGCGG CGAAGCAGAT GGCGGCGCTC 
AAGGCGCTGC TCGAGCGGGT GCTGGTGCGC ACCGCGGCGC TGGCCTTCTA CGGCTGGAGG 
GAGGTGGTCG CCGACGCCAA GCGCTGGCGG GCGGTGCAGG CGGTTACGCA GGAGCGCATG 
CAGCAGCGGC CTGAGCTGGC CGAGGTGGCC ATGTACGCAG CCACCCGCAT GCGCAACTGG 
CAGCTGGCTC TGGCGTTCTA CACCTGGTAC GACAACGCTC GGGAGTCGCG CTACCTCAAG 
AACCGGGCCA GCCAGGCCAT CCTGCTATAC GCCAACCGGC TGCTGTACAA TGCCTGGTCT 
GTATGGCTGG CGCACACGCT GCGTAACCGG CACCTGCGCA AGAAGCTTGA GCGATGCCTG 
AACTCAACGC GGGCAAAGAC CTCGCGCGGC GTCTTTGATG CCTGGCTGGC CGCCGCCAAG 
TACTTGGGCC GGCTGCGCAA GGTGTCTGAC CTGGTCAAGG ACAAGCTGCT GCGCGGCACC 
CTGGTGGGCG CCTTCACCTC CTGGCGCCGC ACGGCGATAC AGTTCCGCCG CCTGCGCGGC 
ATCCTGACCC GCGTCATGTC GCGGGCGCTG TCCACCGCCT GGGAGGCCTG GCGCGATGCC 
GTGGCCGAGC GCCACGAGCG CATGCAGTCC CTGGCCGTCT TTGTAGGGCA CTGGCGCAAC 
CTGCACCTGT CCGCTGCCTT CAACGCGTGG GTGGCACACG TGCACAAGCG CGCCGCCGCC 
CGGCGCCTTT GCCTGCGCGT GCTGGGCCGG CTGCTGGAAT GGGCTTGGTA CGGCTGGCGG 
GAGGCGGTGT TTGAGGCGGG CGTCAGCCGG GGCGCCGACG TGCACGAGGC GAGGCTGCTG 
GCTAGGTCTT GGCGGGGCTG GCGTTGGGCG ACGTCGGAGG GCCGCAAGGC GGCGGCGCTG 
ACCGCGGCGC TGAACCAGGC GGCGGCGCTG ACAGCAATGG GCGTGTGGCG GCGCCAGTGG 
CTGGCGCGTG CGGCGTACCG GCGACTGTAC TGCCGGCGTG CGCTGCTGGG CTGGCACTCG 
CGCACGCAGG AGCTGCGCGC AATGAGGGAG AGGCTGTGGT ACGCGGCGCG CTTCCTCCTG 
AACGGCTGCC TGCTGCGCAG CTTCAGTGCG TGGTGGCAGT ACACGCAGGC CATGTCCATC 
AAGCGAGCCG TGTGGGTGCG CAAGCAGCGC GCCCTGGCGG AGGCCCTGCG GCGCGGCGGC 
GAGCTGGTGG CGGCGCGCAA CGCCGAACTG ATGGAGATTG CGTTCCGCGG CTGGCGCATG 
CAGACGGGGC TGCTGCGCGA GGTGACGCGG CGGCTGCGGT CCATCCAGGG CCGCACCCTG 
GCCACTGCCT TCGGCCAGTG GCGCGAGTAC GCGGCAGTGA AGCGCGCGCG GCTGGAGAAG 
CAGGCGGCTG TGATTCGCTC CCGGTTGTTG GCCCGGCCCT TCCGCACCTG GCGCGCCGCT 
GCCCGCGGCG CAGCCTCCGA GCTGTCTGCC AAGTCCGCGG CGGCGCTCAT GCAGCGGCGC 
GGTGTGCTGC TAGCGGCCAC CTTCCGCGTC TGGCGTGAGC TGCACGGCGT TGTGGGCAGC 
CGCCGGGAGG CTCTGCGCCG CGCACTGGGC GTGGTGCTAC GGGCGGAAGC CGGGGTTCTG 
AAGCAGGCGG TGTGGGGCGC CTGGCGGTGG GCGGTGGCCC GGCGGGGCGA CCTGCTGGAC 
CTGGTGTCGG CGGTGTCGGC GGGGCGTCGG CGGCGTGTGC TGGGCGAGGT GTTCGACGTG 
TGGCTGCAGT ACACGCAAGC CATGCGGCGG GGCGGCATCG ACCCCGGCTC GCCGTACATG 
TCGCCGCGGG AGCGCGGCAC GGAGCGGCGG CTCATCACCC GCATGGCTGC ACTGGCGGGC 
AACGACCAGG CACTGGCGCC GGCGGCTCAC GCCGGCGGCG CCTCCGTCGC CGCAACCGAT 
GCACTGCTGG ACGGGCTATA TCCCTCCACC GGTATGCAGC ACGCTTCGGA ACGGCGGCAG 
GAGCTGTCTG CGTTCCTGGA CTTTGCACGC CGCGCCACCT CGACTGGACG CGCAGGAAGA 
AACGGCCGCA GTGCTGCGCA GCAGGCAGCG CCGCCGGTGT ACGAGCGGGC GGAGAGCATC 
AGCAGCGACC TCAGCCGCGC CAGCACGGGG CTGTCCTCGG GGGCAGGCGG CGCGGACCTG 
ACGGAACGGG CCATGTTCTC CGTGCGGGCC ATGCCGGGTA TGATCTCGCC GGGTAAGGGC 
CGCGGCCCCG CCTCGGGCCT CACGCCCGCC CATATCCAGC TCGCCACCGC GGGTCGTGCG 
GCCCCCGCCC CGCTGTCTGC GCGGCGGCCC AGCGGTCCGC AGGACTTCGC GGGTCTCAGC 
AGTCCCTTCG ATGACCGGCT GGATCTTCGG GAGCTGTACG GCGCTGCGGA CCAGGCGGCG 
GCGGCGGCCC CGGCGCCCTT CGGAGGCTTC CACACCACAG CGGGATACGG TGCACGTGGG 
GCGCAGCTGG CCAGCGGCGC CGCCAGTCCG ATGCCACTCA TGCCGCCGCC TGCGGCCCGT 
GGCGGCTTGG CAATGGCAAC ACCGCTGACC GCGTCTGTGC CGATTGCACC CCGCCGCATT 
GACTTCACCG CTTCCCGCCC TGGATCCGGT GCGGGTGCGG GGCCAGCGAC GGGTGGTGCC 
TACGGCTATG GCGGTCCTGC GGGCCGGGGC CCGGGCACGC CCAGATCCCA GCAATTCGCG 
CAGCCCGCGG AGCCAGCCTA TCCGGACGAC AGCGATGGCG CCAGCACCAC CAGCAGCGAC 
TTCCTGCCTC CGTCTGAGGC GCGGGCCAGG GCGCAGCAGG CGCGGGCGGC CGCAGTAGCA 
ACGCCGCGCA TTGCTTTGGG TGTTGCAGCC ATTCACGGCA CCTTTATTCA GGCCAATCGA 
CCAGAGCACT TCCATGCCGT GCACCTACCC ATTGCTGCGG GCGGGCAGTG GCTGGTCATC 
GGCGGCTGGG GGTGGAGCGA CCACTGGGGG GAGACCGATG TGCTTGGGCT GCTTGGCGCC 
CTGCAGCGCC ACTGCATCCA ACGGCGCTGG CCGGCGCCCG CCTACGTGCT GCTGGCGCCC 
CCCACCACTG TCGAGCATCA GCAGGGCTTG GGTTTCCCAT TCGGCATTCC GGTGCCGTTT 
GACCTGGTAC AGACCGGCGT CGGCGTGCAT CACGCATGCA GCCTCTACAC CAACCTGGCC 
ACCGCAGAGC ACCTCACCGC CGTGTTCCGC GAGATGCGGC CCCTGCCCCA GCGACGCCTA 
CCCCAGCTCG TGGCGCCCGG CGTGCGGCTG CCTGTTGCCG CTCAAGTCGA GCCGCTGCCG 
CACGTCCCGG TCCATGCACC GGGTCAGCGG CTTGCAGCCC TCCCCGCCGG CGCCACCTGC 
ATCCCCGCGG ACGCCAATGC GCCGGCCCAA CCGCTATCGC CGCGTGACTG GGAAGAGCTC 
CTGGGGCACG TGCCGGGAGC ATCCTCCGGC CTGCCGCCCA GCGCAGCGCT CGCCGCGCTG 
CGCTCAGCCC CGCCGCCGCT GGCCCTCAGC GCCGTCCTAG CAGCCGCCGT CGTCTTGCAG 
CGCCACTACC TCACGCCTCA CGACACACAC ACGCCGCCGC CCCAAGTGCT GGCACCTGCA 
GACGCAGGAC CGGTACTGCC GCTGGGGGGG AGTGACGACG CAGAGGACAC GGAGCTGGCG 
CGAATGCTGA TACAGCGGCA GCCCGGCAGC GACCTGCTGG TATTTGCCAC CATGACGTGG 
GGCCCGGAGC AGCAGCTGGC GCTCGACACA GTTAAGGCGG AGTTTGCGCG CGAGGGCCTG 
ATCCTGCGCG CTTACGACCC CTCTCGGCCG CTCATCCTGC ACACGGACTG GTGTGAGGAC 
GGCGTCAGTG GCGTGCTAGG GCAGCTGGAT GACGACGCGC GGGAGTACAT GGTCGCCTGT 
GTTAGCCGCA CCTGCAACGT GCATGAGCGG CGCTACGGCA GCTACAAGGG CGAGCTGCTG 
GCGGCGGTTT GGGCCATTCA GACCTTGCGC CCCTACCTGC ACGCCACGCC GTTCACGCTG 
GTGACAGACC ATGCGCCCCT GGAGTGGCTG ATGTCACAGC CGGAGCTAAC CGGACAGGCT 
GCGCGCTGGG CGATGATACT GCAGCAGTAC AGCTTCTCGC GCCACCACAA CGCCTGCCGT 
GCTGCTGCTG CACCCGGCGC AGCCCTGGGC GCCTGCGTCA CGCCTGCAGT TCGCGTGTTT 
GCAGGCCACG CTCACGCCGG CAGGCGACAC CACGGTACCC AGCCCCGTCC TGCGCCGCGC 
CTGCGGCTGC GTACATCAGC GCTGCACGGC AGCGTGGTGG GAGTCAGCAC CTTCCTGCGG 
CAACATGCGC TCATCTTTGC CGCGGAAACA GATGCAATCT CGGTGCTGGA GGTCCCGGGA 
GGGTTGGCAC CTGGCCTGCA GGCGTGCCTG CGGCTAGGCT GGCGCATCCG GCGCTACTGC 
AACCTCGACA GCAGTGGAAC GGTAACTGAC AGCGCTTACA ATCGACAAGC GGCACCCGCC 
AGCGTTATCC AGCACCCGTG CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001695244.1
CDS	1..8967
Translation	MTINNPLRLSSEVSPLRHVLDITARSQGKDLTAYETYRALPSPTRPYSGYDYAVVAEIIRAAEVTVDKAHKKGLGSGIVTLQSVLQAYEHVLPRHGVKADEDTYYYRLLLKLSLDPALDWWIKLNRETGTTGGRAAFFSNAGSDVTSMGRSHSFYRGDTSPTRVQPRTSGAGASDYSASHSAAPTFRSSIPGNGAADGGSRVPGRNSPYRSTASLSSMPGYQQQQETAAQAAAREAAARANAAAQAARDAIEAARQAAATAHLPSSRFMFNTPPMTPAMGPVPPPSARTEGGASGWGDGSMGGAGSIVPYSVSEGGATGPGGASQYGPGSRYGPGSPHGTTAPSSVRGTYGISEHDPLSPDASAYGAGSRGTRSGGGADGGSMGGAVGPGATRASSGGGGGPGRVSAAQLAELDEPVLSEEAEAYLDYNRLARAFRMWRKNTFARKASRFERDHALKNWAVATSFWAVHLLRRCFARWRAEGPSKTALALQLWAGHSLSACFWRWLSLTRYLRGKGTECDGHWRVRTLRRCLRMWRLYTRSQQLKADNVEKALGFWVGRSLHICFRVWRQFAANQLRKNEAVQKAVGHWAGKSLRACFEVWREFTMLQQHKSVATARALRHWTGTTLRSCFALWRLLVEDAHSNWDQATQHLMRRMLLKWRIAAKQLTRMSAAYHAIADATARRVMSQAMRTLLSAHAFRHETANRLRAAVARMRNSLLSAAFNKWSEARQAGQMKRERMALALKWWSSRGLARSWNTWREAVAASQRATKAARANAQRILRPLLRDTFYAWLDWHDVRLQKEVRAVAARRTMQLRQMRLVVLGWRGLVLERRHSLVSFQRIMRNEVQRIMSTAFQRWYREHDNLRRAAEARLTVKRNATKRCLRHWRALAQAQRAHREWRLERLRLASEQRQRRLLVAWQVVAGLLAEHNRLVKSSLFKLHRRQAHSVLAAWRDRVLHSVAKRMKLLAGLLYWEQQVKGRAWLAWRQRQHGWRIKAAKGELAGRHWRTRRLALCFAVFLALWQAYQGRLTRALLFRSRRNDIIKADVLDEWRRLVGWLQWKNDKIRRALAHHRSKLGGGAFYGWYAVTRHSIATKRAVAAAVLHWVRRRRAAAFVWWRNWTAYRRDLRVRGAGYALVRAARAKEKVVLAFKANVIRKARIAGAVAHRQRYMTRLALSGWLLRTLLAADFVRRLKAVGAQLNVQLVVDGLEAWREYTIYRRTKKGMLHAALRYWRLSRQRAAMDAMRWYTTRRQLKRAVLLRGRTGAAARALRAWRDEAEYRRRLRDRYAMLASGSRHAALRRGLDMWRDYIANRASKKGLREQALRHWLRGTAGRVFRSWRDYQRHLRHAEERAGDIVQRWRKRDAAEALAAFAEYAAHRRRKRTADAMSRRSRARTALQAWREYMKLRTAEGFWSRSRLNDAIGAWRHYANHRRRLKELWGRTRRGRLASALSSWRDYATYSARLKTVSNAAKQMAALKALLERVLVRTAALAFYGWREVVADAKRWRAVQAVTQERMQQRPELAEVAMYAATRMRNWQLALAFYTWYDNARESRYLKNRASQAILLYANRLLYNAWSVWLAHTLRNRHLRKKLERCLNSTRAKTSRGVFDAWLAAAKYLGRLRKVSDLVKDKLLRGTLVGAFTSWRRTAIQFRRLRGILTRVMSRALSTAWEAWRDAVAERHERMQSLAVFVGHWRNLHLSAAFNAWVAHVHKRAAARRLCLRVLGRLLEWAWYGWREAVFEAGVSRGADVHEARLLARSWRGWRWATSEGRKAAALTAALNQAAALTAMGVWRRQWLARAAYRRLYCRRALLGWHSRTQELRAMRERLWYAARFLLNGCLLRSFSAWWQYTQAMSIKRAVWVRKQRALAEALRRGGELVAARNAELMEIAFRGWRMQTGLLREVTRRLRSIQGRTLATAFGQWREYAAVKRARLEKQAAVIRSRLLARPFRTWRAAARGAASELSAKSAAALMQRRGVLLAATFRVWRELHGVVGSRREALRRALGVVLRAEAGVLKQAVWGAWRWAVARRGDLLDLVSAVSAGRRRRVLGEVFDVWLQYTQAMRRGGIDPGSPYMSPRERGTERRLITRMAALAGNDQALAPAAHAGGASVAATDALLDGLYPSTGMQHASERRQELSAFLDFARRATSTGRAGRNGRSAAQQAAPPVYERAESISSDLSRASTGLSSGAGGADLTERAMFSVRAMPGMISPGKGRGPASGLTPAHIQLATAGRAAPAPLSARRPSGPQDFAGLSSPFDDRLDLRELYGAADQAAAAAPAPFGGFHTTAGYGARGAQLASGAASPMPLMPPPAARGGLAMATPLTASVPIAPRRIDFTASRPGSGAGAGPATGGAYGYGGPAGRGPGTPRSQQFAQPAEPAYPDDSDGASTTSSDFLPPSEARARAQQARAAAVATPRIALGVAAIHGTFIQANRPEHFHAVHLPIAAGGQWLVIGGWGWSDHWGETDVLGLLGALQRHCIQRRWPAPAYVLLAPPTTVEHQQGLGFPFGIPVPFDLVQTGVGVHHACSLYTNLATAEHLTAVFREMRPLPQRRLPQLVAPGVRLPVAAQVEPLPHVPVHAPGQRLAALPAGATCIPADANAPAQPLSPRDWEELLGHVPGASSGLPPSAALAALRSAPPPLALSAVLAAAVVLQRHYLTPHDTHTPPPQVLAPADAGPVLPLGGSDDAEDTELARMLIQRQPGSDLLVFATMTWGPEQQLALDTVKAEFAREGLILRAYDPSRPLILHTDWCEDGVSGVLGQLDDDAREYMVACVSRTCNVHERRYGSYKGELLAAVWAIQTLRPYLHATPFTLVTDHAPLEWLMSQPELTGQAARWAMILQQYSFSRHHNACRAAAAPGAALGACVTPAVRVFAGHAHAGRRHHGTQPRPAPRLRLRTSALHGSVVGVSTFLRQHALIFAAETDAISVLEVPGGLAPGLQACLRLGWRIRRYCNLDSSGTVTDSAYNRQAAPASVIQHPCL

Target ORF information:

RefSeq Version	XM_001695192.1
Organism	Chlamydomonas reinhardtii
Definition	Chlamydomonas reinhardtii spindle pole body protein (SFI1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001695192.1

ORF Insert Sequence:

ATGACGATCA ACAATCCCCT TAGACTGAGC TCGGAGGTGT CGCCGCTGCG ACATGTGCTG 
GATATCACAG CACGGTCCCA GGGGAAGGAT TTGACTGCAT ATGAGACCTA TCGGGCTCTC 
CCCAGCCCGA CGCGGCCATA CAGCGGCTAC GACTACGCCG TGGTCGCCGA AATCATCCGG 
GCTGCGGAGG TCACGGTCGA CAAGGCGCAC AAGAAGGGGC TCGGCTCAGG CATCGTCACG 
CTGCAGTCAG TGCTGCAGGC ATACGAGCAC GTGCTGCCGC GCCATGGTGT GAAGGCGGAT 
GAGGACACGT ACTACTACCG CCTGCTGCTC AAGCTCAGCC TAGACCCAGC GCTGGACTGG 
TGGATCAAGC TAAACCGGGA GACTGGGACT ACGGGCGGTC GCGCCGCGTT CTTCAGCAAT 
GCCGGCAGCG ACGTGACGTC CATGGGGCGG TCGCACAGCT TCTACCGCGG CGACACCTCG 
CCCACACGGG TCCAGCCCCG CACCTCGGGT GCCGGCGCTA GTGACTATTC GGCCAGCCAC 
AGCGCTGCGC CCACCTTCCG CAGCAGCATA CCGGGCAACG GCGCAGCGGA CGGGGGAAGT 
CGGGTGCCCG GCCGCAACTC CCCCTACCGG TCCACGGCCT CTCTCTCCAG CATGCCCGGC 
TACCAGCAGC AGCAAGAGAC CGCCGCACAG GCAGCTGCGC GGGAGGCGGC GGCGCGCGCG 
AATGCTGCGG CGCAAGCCGC ACGGGATGCG ATTGAAGCGG CGCGGCAGGC GGCCGCCACG 
GCGCACCTAC CATCTTCGCG CTTCATGTTC AACACACCCC CTATGACGCC GGCTATGGGA 
CCTGTACCGC CACCGTCAGC TCGGACCGAA GGTGGCGCCA GCGGCTGGGG GGATGGCAGT 
ATGGGGGGCG CGGGCAGCAT CGTGCCGTAC AGCGTGTCGG AGGGCGGCGC CACGGGCCCG 
GGAGGGGCTT CCCAATATGG ACCGGGAAGC AGATACGGGC CAGGGAGCCC CCATGGCACG 
ACTGCGCCTA GCAGCGTGCG GGGCACCTAC GGCATCAGCG AGCACGACCC CCTCAGTCCT 
GATGCGTCGG CGTATGGGGC TGGATCTCGG GGCACCCGGA GTGGCGGCGG AGCCGATGGT 
GGCAGTATGG GTGGCGCAGT AGGGCCGGGC GCCACCCGTG CCAGTAGCGG AGGCGGCGGT 
GGTCCCGGCC GGGTCAGCGC CGCCCAGCTG GCGGAGCTGG ACGAGCCGGT GCTGAGCGAG 
GAGGCGGAGG CTTACCTGGA CTACAATCGC CTGGCGCGCG CCTTCAGGAT GTGGCGCAAG 
AACACCTTTG CCCGCAAGGC GTCCAGGTTT GAGCGCGACC ATGCGCTCAA AAATTGGGCT 
GTGGCGACCT CGTTCTGGGC GGTGCACCTG CTGCGCCGCT GCTTCGCGCG CTGGCGTGCA 
GAAGGGCCGA GCAAGACGGC GCTGGCGCTG CAGCTTTGGG CGGGCCACAG CCTGTCAGCC 
TGCTTCTGGC GGTGGCTGAG CCTCACGCGC TACCTCCGGG GAAAAGGGAC CGAATGCGAT 
GGGCACTGGC GCGTGCGGAC GCTGCGCCGC TGCCTGCGCA TGTGGCGTCT CTACACTCGG 
TCCCAGCAGC TCAAGGCCGA CAACGTGGAG AAGGCACTGG GCTTTTGGGT TGGCCGTTCC 
CTGCACATCT GCTTCCGCGT GTGGCGCCAG TTTGCGGCAA ACCAGCTGCG CAAGAATGAG 
GCGGTGCAGA AGGCGGTCGG GCACTGGGCG GGCAAGTCGC TGCGGGCCTG CTTTGAGGTC 
TGGCGCGAGT TCACAATGCT TCAGCAGCAC AAGTCGGTGG CGACCGCCCG GGCGCTGCGC 
CACTGGACCG GCACCACGCT GCGCAGCTGC TTCGCGCTGT GGCGGCTGCT TGTCGAGGAC 
GCGCACAGCA ACTGGGACCA GGCCACACAG CACTTGATGC GACGGATGCT GCTCAAGTGG 
CGCATTGCGG CCAAGCAGCT AACCCGCATG AGCGCTGCCT ACCACGCCAT CGCGGACGCT 
ACTGCGCGTC GGGTCATGTC GCAGGCCATG CGGACGCTGC TGTCGGCGCA CGCCTTCCGG 
CACGAGACCG CTAACCGGCT CCGTGCGGCG GTGGCGCGCA TGCGCAACTC GCTTCTGTCC 
GCCGCCTTCA ACAAGTGGAG CGAGGCGCGC CAAGCCGGCC AGATGAAGCG GGAGCGGATG 
GCGTTGGCGC TCAAGTGGTG GTCGTCCAGA GGCCTGGCTC GCAGCTGGAA CACCTGGCGC 
GAGGCGGTGG CTGCAAGCCA GCGCGCGACG AAGGCGGCGC GGGCGAATGC GCAGCGCATC 
CTGCGGCCGC TGCTACGTGA CACCTTCTAC GCCTGGCTGG ACTGGCACGA CGTCAGGTTG 
CAGAAAGAGG TCCGCGCGGT AGCGGCGCGG CGAACCATGC AGCTGCGGCA GATGCGGCTG 
GTGGTGCTGG GCTGGCGGGG GCTGGTGCTG GAGCGGCGGC ACAGCCTGGT GTCGTTCCAG 
CGCATCATGC GGAATGAGGT GCAGCGGATT ATGAGCACAG CCTTCCAGCG ATGGTACCGC 
GAGCATGACA ACCTGCGACG TGCGGCAGAG GCGCGGCTGA CGGTGAAGCG AAACGCGACC 
AAACGCTGCC TGCGCCACTG GCGGGCGCTA GCGCAGGCAC AGCGCGCGCA CAGGGAGTGG 
CGGCTGGAGC GGCTGCGGCT AGCGAGCGAG CAGCGGCAAC GCCGGCTGCT GGTGGCGTGG 
CAGGTGGTCG CGGGGCTGCT GGCGGAGCAC AACCGGCTAG TCAAGTCCAG TTTGTTCAAG 
CTGCACCGGC GGCAGGCGCA CTCTGTGCTG GCGGCCTGGC GCGACCGCGT GCTGCACTCG 
GTGGCCAAGC GCATGAAGCT GCTGGCGGGG CTGCTGTACT GGGAGCAGCA GGTGAAGGGC 
CGCGCCTGGC TGGCCTGGCG GCAGCGCCAG CACGGCTGGC GCATCAAGGC CGCCAAGGGG 
GAGCTGGCGG GCCGGCACTG GCGCACGCGG CGCCTGGCGC TATGCTTCGC GGTGTTTTTG 
GCGCTGTGGC AAGCGTACCA GGGTCGCCTG ACGCGCGCGC TGCTGTTCCG GTCCCGGCGC 
AACGACATCA TCAAGGCGGA TGTGCTGGAC GAATGGCGGC GCCTGGTGGG CTGGCTGCAG 
TGGAAGAACG ACAAGATCCG CAGGGCGCTG GCACACCATC GCTCCAAGCT CGGGGGAGGC 
GCCTTCTACG GCTGGTACGC AGTCACCCGT CACTCCATCG CCACTAAGCG GGCCGTGGCC 
GCGGCTGTGC TCCACTGGGT TCGCCGCCGC CGCGCCGCCG CCTTCGTGTG GTGGCGCAAT 
TGGACCGCCT ACCGCCGCGA CCTGCGCGTG CGCGGTGCGG GCTACGCGCT TGTGCGGGCG 
GCGCGGGCCA AGGAGAAGGT CGTGCTGGCG TTCAAGGCCA ACGTGATCCG CAAGGCTCGC 
ATCGCCGGCG CGGTGGCTCA CCGGCAGCGC TACATGACGC GCCTAGCGCT GTCGGGCTGG 
CTGCTGCGGA CGCTGCTGGC AGCGGATTTC GTGCGGAGGC TGAAGGCTGT GGGCGCGCAG 
CTCAATGTCC AGCTGGTGGT GGACGGGCTG GAGGCCTGGC GCGAGTACAC CATCTACCGC 
CGCACCAAGA AGGGCATGCT GCACGCAGCT CTGCGCTACT GGCGCCTCAG TCGGCAGCGC 
GCGGCCATGG ACGCCATGCG CTGGTACACC ACCCGGCGAC AGCTCAAGCG CGCGGTGCTG 
CTGCGCGGCC GCACTGGCGC CGCGGCAAGG GCGCTGCGGG CTTGGCGCGA CGAGGCGGAG 
TACCGCCGCC GCCTGCGCGA CCGCTACGCG ATGCTGGCCT CCGGCAGCCG GCACGCGGCG 
CTGCGGCGGG GGCTGGACAT GTGGCGCGAC TACATCGCGA ACCGCGCCTC AAAGAAGGGG 
CTGCGGGAGC AGGCGCTGCG GCACTGGCTG CGAGGCACGG CTGGGCGGGT GTTCCGCAGC 
TGGCGGGACT ACCAGCGCCA CCTGCGGCAC GCCGAGGAGC GCGCTGGCGA CATCGTGCAG 
CGTTGGCGGA AGCGGGATGC GGCTGAGGCG CTGGCGGCGT TCGCCGAGTA TGCCGCGCAC 
CGCCGCCGCA AGCGCACCGC GGACGCGATG TCACGTCGCA GCCGCGCCCG CACCGCGCTG 
CAGGCGTGGC GCGAGTACAT GAAGCTGCGC ACGGCGGAAG GCTTCTGGAG CCGCAGCCGC 
CTGAACGATG CCATCGGGGC ATGGCGGCAC TACGCCAACC ACCGCCGTCG GCTGAAAGAG 
CTGTGGGGCC GCACCCGCCG TGGCCGGCTG GCCTCCGCGC TGTCCAGCTG GCGCGACTAC 
GCCACCTACT CGGCCCGCCT CAAGACCGTG TCCAACGCGG CGAAGCAGAT GGCGGCGCTC 
AAGGCGCTGC TCGAGCGGGT GCTGGTGCGC ACCGCGGCGC TGGCCTTCTA CGGCTGGAGG 
GAGGTGGTCG CCGACGCCAA GCGCTGGCGG GCGGTGCAGG CGGTTACGCA GGAGCGCATG 
CAGCAGCGGC CTGAGCTGGC CGAGGTGGCC ATGTACGCAG CCACCCGCAT GCGCAACTGG 
CAGCTGGCTC TGGCGTTCTA CACCTGGTAC GACAACGCTC GGGAGTCGCG CTACCTCAAG 
AACCGGGCCA GCCAGGCCAT CCTGCTATAC GCCAACCGGC TGCTGTACAA TGCCTGGTCT 
GTATGGCTGG CGCACACGCT GCGTAACCGG CACCTGCGCA AGAAGCTTGA GCGATGCCTG 
AACTCAACGC GGGCAAAGAC CTCGCGCGGC GTCTTTGATG CCTGGCTGGC CGCCGCCAAG 
TACTTGGGCC GGCTGCGCAA GGTGTCTGAC CTGGTCAAGG ACAAGCTGCT GCGCGGCACC 
CTGGTGGGCG CCTTCACCTC CTGGCGCCGC ACGGCGATAC AGTTCCGCCG CCTGCGCGGC 
ATCCTGACCC GCGTCATGTC GCGGGCGCTG TCCACCGCCT GGGAGGCCTG GCGCGATGCC 
GTGGCCGAGC GCCACGAGCG CATGCAGTCC CTGGCCGTCT TTGTAGGGCA CTGGCGCAAC 
CTGCACCTGT CCGCTGCCTT CAACGCGTGG GTGGCACACG TGCACAAGCG CGCCGCCGCC 
CGGCGCCTTT GCCTGCGCGT GCTGGGCCGG CTGCTGGAAT GGGCTTGGTA CGGCTGGCGG 
GAGGCGGTGT TTGAGGCGGG CGTCAGCCGG GGCGCCGACG TGCACGAGGC GAGGCTGCTG 
GCTAGGTCTT GGCGGGGCTG GCGTTGGGCG ACGTCGGAGG GCCGCAAGGC GGCGGCGCTG 
ACCGCGGCGC TGAACCAGGC GGCGGCGCTG ACAGCAATGG GCGTGTGGCG GCGCCAGTGG 
CTGGCGCGTG CGGCGTACCG GCGACTGTAC TGCCGGCGTG CGCTGCTGGG CTGGCACTCG 
CGCACGCAGG AGCTGCGCGC AATGAGGGAG AGGCTGTGGT ACGCGGCGCG CTTCCTCCTG 
AACGGCTGCC TGCTGCGCAG CTTCAGTGCG TGGTGGCAGT ACACGCAGGC CATGTCCATC 
AAGCGAGCCG TGTGGGTGCG CAAGCAGCGC GCCCTGGCGG AGGCCCTGCG GCGCGGCGGC 
GAGCTGGTGG CGGCGCGCAA CGCCGAACTG ATGGAGATTG CGTTCCGCGG CTGGCGCATG 
CAGACGGGGC TGCTGCGCGA GGTGACGCGG CGGCTGCGGT CCATCCAGGG CCGCACCCTG 
GCCACTGCCT TCGGCCAGTG GCGCGAGTAC GCGGCAGTGA AGCGCGCGCG GCTGGAGAAG 
CAGGCGGCTG TGATTCGCTC CCGGTTGTTG GCCCGGCCCT TCCGCACCTG GCGCGCCGCT 
GCCCGCGGCG CAGCCTCCGA GCTGTCTGCC AAGTCCGCGG CGGCGCTCAT GCAGCGGCGC 
GGTGTGCTGC TAGCGGCCAC CTTCCGCGTC TGGCGTGAGC TGCACGGCGT TGTGGGCAGC 
CGCCGGGAGG CTCTGCGCCG CGCACTGGGC GTGGTGCTAC GGGCGGAAGC CGGGGTTCTG 
AAGCAGGCGG TGTGGGGCGC CTGGCGGTGG GCGGTGGCCC GGCGGGGCGA CCTGCTGGAC 
CTGGTGTCGG CGGTGTCGGC GGGGCGTCGG CGGCGTGTGC TGGGCGAGGT GTTCGACGTG 
TGGCTGCAGT ACACGCAAGC CATGCGGCGG GGCGGCATCG ACCCCGGCTC GCCGTACATG 
TCGCCGCGGG AGCGCGGCAC GGAGCGGCGG CTCATCACCC GCATGGCTGC ACTGGCGGGC 
AACGACCAGG CACTGGCGCC GGCGGCTCAC GCCGGCGGCG CCTCCGTCGC CGCAACCGAT 
GCACTGCTGG ACGGGCTATA TCCCTCCACC GGTATGCAGC ACGCTTCGGA ACGGCGGCAG 
GAGCTGTCTG CGTTCCTGGA CTTTGCACGC CGCGCCACCT CGACTGGACG CGCAGGAAGA 
AACGGCCGCA GTGCTGCGCA GCAGGCAGCG CCGCCGGTGT ACGAGCGGGC GGAGAGCATC 
AGCAGCGACC TCAGCCGCGC CAGCACGGGG CTGTCCTCGG GGGCAGGCGG CGCGGACCTG 
ACGGAACGGG CCATGTTCTC CGTGCGGGCC ATGCCGGGTA TGATCTCGCC GGGTAAGGGC 
CGCGGCCCCG CCTCGGGCCT CACGCCCGCC CATATCCAGC TCGCCACCGC GGGTCGTGCG 
GCCCCCGCCC CGCTGTCTGC GCGGCGGCCC AGCGGTCCGC AGGACTTCGC GGGTCTCAGC 
AGTCCCTTCG ATGACCGGCT GGATCTTCGG GAGCTGTACG GCGCTGCGGA CCAGGCGGCG 
GCGGCGGCCC CGGCGCCCTT CGGAGGCTTC CACACCACAG CGGGATACGG TGCACGTGGG 
GCGCAGCTGG CCAGCGGCGC CGCCAGTCCG ATGCCACTCA TGCCGCCGCC TGCGGCCCGT 
GGCGGCTTGG CAATGGCAAC ACCGCTGACC GCGTCTGTGC CGATTGCACC CCGCCGCATT 
GACTTCACCG CTTCCCGCCC TGGATCCGGT GCGGGTGCGG GGCCAGCGAC GGGTGGTGCC 
TACGGCTATG GCGGTCCTGC GGGCCGGGGC CCGGGCACGC CCAGATCCCA GCAATTCGCG 
CAGCCCGCGG AGCCAGCCTA TCCGGACGAC AGCGATGGCG CCAGCACCAC CAGCAGCGAC 
TTCCTGCCTC CGTCTGAGGC GCGGGCCAGG GCGCAGCAGG CGCGGGCGGC CGCAGTAGCA 
ACGCCGCGCA TTGCTTTGGG TGTTGCAGCC ATTCACGGCA CCTTTATTCA GGCCAATCGA 
CCAGAGCACT TCCATGCCGT GCACCTACCC ATTGCTGCGG GCGGGCAGTG GCTGGTCATC 
GGCGGCTGGG GGTGGAGCGA CCACTGGGGG GAGACCGATG TGCTTGGGCT GCTTGGCGCC 
CTGCAGCGCC ACTGCATCCA ACGGCGCTGG CCGGCGCCCG CCTACGTGCT GCTGGCGCCC 
CCCACCACTG TCGAGCATCA GCAGGGCTTG GGTTTCCCAT TCGGCATTCC GGTGCCGTTT 
GACCTGGTAC AGACCGGCGT CGGCGTGCAT CACGCATGCA GCCTCTACAC CAACCTGGCC 
ACCGCAGAGC ACCTCACCGC CGTGTTCCGC GAGATGCGGC CCCTGCCCCA GCGACGCCTA 
CCCCAGCTCG TGGCGCCCGG CGTGCGGCTG CCTGTTGCCG CTCAAGTCGA GCCGCTGCCG 
CACGTCCCGG TCCATGCACC GGGTCAGCGG CTTGCAGCCC TCCCCGCCGG CGCCACCTGC 
ATCCCCGCGG ACGCCAATGC GCCGGCCCAA CCGCTATCGC CGCGTGACTG GGAAGAGCTC 
CTGGGGCACG TGCCGGGAGC ATCCTCCGGC CTGCCGCCCA GCGCAGCGCT CGCCGCGCTG 
CGCTCAGCCC CGCCGCCGCT GGCCCTCAGC GCCGTCCTAG CAGCCGCCGT CGTCTTGCAG 
CGCCACTACC TCACGCCTCA CGACACACAC ACGCCGCCGC CCCAAGTGCT GGCACCTGCA 
GACGCAGGAC CGGTACTGCC GCTGGGGGGG AGTGACGACG CAGAGGACAC GGAGCTGGCG 
CGAATGCTGA TACAGCGGCA GCCCGGCAGC GACCTGCTGG TATTTGCCAC CATGACGTGG 
GGCCCGGAGC AGCAGCTGGC GCTCGACACA GTTAAGGCGG AGTTTGCGCG CGAGGGCCTG 
ATCCTGCGCG CTTACGACCC CTCTCGGCCG CTCATCCTGC ACACGGACTG GTGTGAGGAC 
GGCGTCAGTG GCGTGCTAGG GCAGCTGGAT GACGACGCGC GGGAGTACAT GGTCGCCTGT 
GTTAGCCGCA CCTGCAACGT GCATGAGCGG CGCTACGGCA GCTACAAGGG CGAGCTGCTG 
GCGGCGGTTT GGGCCATTCA GACCTTGCGC CCCTACCTGC ACGCCACGCC GTTCACGCTG 
GTGACAGACC ATGCGCCCCT GGAGTGGCTG ATGTCACAGC CGGAGCTAAC CGGACAGGCT 
GCGCGCTGGG CGATGATACT GCAGCAGTAC AGCTTCTCGC GCCACCACAA CGCCTGCCGT 
GCTGCTGCTG CACCCGGCGC AGCCCTGGGC GCCTGCGTCA CGCCTGCAGT TCGCGTGTTT 
GCAGGCCACG CTCACGCCGG CAGGCGACAC CACGGTACCC AGCCCCGTCC TGCGCCGCGC 
CTGCGGCTGC GTACATCAGC GCTGCACGGC AGCGTGGTGG GAGTCAGCAC CTTCCTGCGG 
CAACATGCGC TCATCTTTGC CGCGGAAACA GATGCAATCT CGGTGCTGGA GGTCCCGGGA 
GGGTTGGCAC CTGGCCTGCA GGCGTGCCTG CGGCTAGGCT GGCGCATCCG GCGCTACTGC 
AACCTCGACA GCAGTGGAAC GGTAACTGAC AGCGCTTACA ATCGACAAGC GGCACCCGCC 
AGCGTTATCC AGCACCCGTG CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Science (New York, N.Y.)318(5848)245-50(2007 Oct)
Merchant SS,Prochnik SE,Vallon O,Harris EH,Karpowicz SJ,Witman GB,Terry A,Salamov A,Fritz-Laylin LK,Maréchal-Drouard L,Marshall WF,Qu LH,Nelson DR,Sanderfoot AA,Spalding MH,Kapitonov VV,Ren Q,Ferris P,Lindquist E,Shapiro H,Lucas SM,Grimwood J,Schmutz J,Cardol P,Cerutti H,Chanfreau G,Chen CL,Cognat V,Croft MT,Dent R,Dutcher S,Fernández E,Fukuzawa H,González-Ballester D,González-Halphen D,Hallmann A,Hanikenne M,Hippler M,Inwood W,Jabbari K,Kalanon M,Kuras R,Lefebvre PA,Lemaire SD,Lobanov AV,Lohr M,Manuell A,Meier I,Mets L,Mittag M,Mittelmeier T,Moroney JV,Moseley J,Napoli C,Nedelcu AM,Niyogi K,Novoselov SV,Paulsen IT,Pazour G,Purton S,Ral JP,Riaño-Pachón DM,Riekhof W,Rymarquis L,Schroda M,Stern D,Umen J,Willows R,Wilson N,Zimmer SL,Allmer J,Balk J,Bisova K,Chen CJ,Elias M,Gendler K,Hauser C,Lamb MR,Ledford H,Long JC,Minagawa J,Page MD,Pan J,Pootakham W,Roje S,Rose A,Stahlberg E,Terauchi AM,Yang P,Ball S,Bowler C,Dieckmann CL,Gladyshev VN,Green P,Jorgensen R,Mayfield S,Mueller-Roeber B,Rajamani S,Sayre RT,Brokstein P,Dubchak I,Goodstein D,Hornick L,Huang YW,Jhaveri J,Luo Y,Martínez D,Ngau WC,Otillar B,Poliakov A,Porter A,Szajkowski L,Werner G,Zhou K,Grigoriev IV,Rokhsar DS,Grossman AR

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SFI1 cDNA ORF clone, Chlamydomonas reinhardtii

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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