MAPKKK2 cDNA ORF clone, Chlamydomonas reinhardtii

Gene
Clones
gRNAs

Summary

Gene Symbol	MAPKKK2
Entrez Gene ID	5720124
Full Name	mitogen activated protein kinase kinase kinase 2
Gene Type	protein-coding
Organism	Chlamydomonas reinhardtii

mRNA and Protein(s)

mRNA	Protein	Name
XM_001694541.1	XP_001694593.1	mitogen activated protein kinase kinase kinase 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Maréchal-Drouard L, Marshall WF, Qu LH, Nelson DR, Sanderfoot AA, Spalding MH, Kapitonov VV, Ren Q, Ferris P, Lindquist E, Shapiro H, Lucas SM, Grimwood J, Schmutz J, Cardol P, Cerutti H, Chanfreau G, Chen CL, Cognat V, Croft MT, Dent R, Dutcher S, Fernández E, Fukuzawa H, González-Ballester D, González-Halphen D, Hallmann A, Hanikenne M, Hippler M, Inwood W, Jabbari K, Kalanon M, Kuras R, Lefebvre PA, Lemaire SD, Lobanov AV, Lohr M, Manuell A, Meier I, Mets L, Mittag M, Mittelmeier T, Moroney JV, Moseley J, Napoli C, Nedelcu AM, Niyogi K, Novoselov SV, Paulsen IT, Pazour G, Purton S, Ral JP, Riaño-Pachón DM, Riekhof W, Rymarquis L, Schroda M, Stern D, Umen J, Willows R, Wilson N, Zimmer SL, Allmer J, Balk J, Bisova K, Chen CJ, Elias M, Gendler K, Hauser C, Lamb MR, Ledford H, Long JC, Minagawa J, Page MD, Pan J, Pootakham W, Roje S, Rose A, Stahlberg E, Terauchi AM, Yang P, Ball S, Bowler C, Dieckmann CL, Gladyshev VN, Green P, Jorgensen R, Mayfield S, Mueller-Roeber B, Rajamani S, Sayre RT, Brokstein P, Dubchak I, Goodstein D, Hornick L, Huang YW, Jhaveri J, Luo Y, Martínez D, Ngau WC, Otillar B, Poliakov A, Porter A, Szajkowski L, Werner G, Zhou K, Grigoriev IV, Rokhsar DS, Grossman AR
Science (New York, N.Y.)318(5848)245-50(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAPKKK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAPKKK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh03309	XM_001694541.1 Latest version!	Chlamydomonas reinhardtii mitogen activated protein kinase kinase kinase 2 (MAPKKK2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAPKKK2 ( XM_001694541.1 ) cDNA ORF clone, Chlamydomonas reinhardtii -> XP_001694593.1 Chlamydomonas reinhardtii mitogen activated protein kinase kinase kinase 2 (MAPKKK2), partial mRNA.

Price & Availability↑

CloneID	OCh03309
Clone ID Related Accession (Same CDS sequence)	XM_001694541.1
Accession Version	XM_001694541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1503849600000
Organism	Chlamydomonas reinhardtii
Product	mitogen activated protein kinase kinase kinase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843666). COMPLETENESS: incomplete on both ends.

ATGAATTCTG GCACCACCGT TGGCGGGAGC AGCGTCAAGC CTGTCAACTC GATAAGGACC 
GCTGCGCGGC CCGGGTCGAG GCGCTTGCTA CTGCTGTTGT TTCTGATTGC ATCCAACGGG 
AAGCAATCTG AGCTGGTGCT CGCTGCAGAC GTGCAGGATA TCTGGTCCGG ACTTTCAGGC 
ACGGAGCCAT CGTCCCAAGG AGAGCGCGGG GCCGCGCCGT CCCGTGGCGG GCTGTGGACG 
TGGGATGAAT CTCGGCTGTG CCTTGCGGCG CTGCTGACCC AGCAACCAAC GGCTGCGGCT 
GCGGCGGGGG CAGCTGTGGG GGACGCGGCC ACCCCGGCAC CTGTGCTCGT GAGCCTGGAG 
GGGCGGCCGT GCACCCAGCG CCTGTCGTAC GCGTGCGTGC CAGATGCCGA CGTTCGAGCG 
GCGGGCCTGA CGCCCTCCTC AGAGTGGTTC CGTGCATATG TCCGGAATGG CTCCACGGCT 
GGTGCGGCTG CGGGTGCTAC AGCCGCTACC GCTGCGCTGG TCGTCGGCAG CAGCAGCCCC 
CATCTGTTGG GGCCTGAGCG CAGTGGGCTG GTGCTGCTGC GCTCGGGTTT CGCCTTGGGA 
GGCACGCACG TGCCGCTGGG GCTCAAAGCG GAGGAGTGGG GTCCGCAGCT TATGAACAAC 
TCTATGCAGG GGGCATTTGG AACGGAACCT TACTACAGTA TTGAAGCTCC AGCGCAGCTG 
TACACATTGG CGTTCGATGA CTCGGATCGG TTTTGGAGCC GCGGTCCCGT GGTCTCAGCC 
TTTACAAGGT CGTATAATGG GAGGCTGTTG GCGTTGCGGC CGGTGCGAGA GGCCGAGGTT 
GATGCAGCCA TTTCTACTGA CACGTCGGCA GCAGCTGCTG GGAACACCAG CAGTGACAGT 
GACAGCCTCC TCCGAGAAGG CGTGTTCGGC GCAAGCCGGC CTGATCTGGA TGGCATCAGC 
AGTGCGCCGT ACGAGACGCA TCACGTGGGG TCGTACAATG ACTACGTGGT GACTGTGCAG 
GGCTGCTTTC GCACGCTGGC CGTGGAGGTG CTTCTGCTGG TCACTCGCAG CGGGCGGCGC 
TACCGGCTGG GCCGCGGCAG CTGCACCGTG TGGTTCAGGG AGGACGCGCC GCCCGGTGGC 
TACCTGGCTG GCTTTGCGGG CCTTCAGCTG GACGTGGGAT ATTGGAGCAG CCAGCCCCCG 
ATGCGTTGGG CCGAAGGCCA GGTGCCCAAC GCACCGTGGC TCATGCAGCT GGAGCTGGTG 
TGGGCCGCGC CTGCCGGATC GCAACCTCCC GCATACACAC AGCGGCTGCC GGCCCTGCTG 
CGGCACTCCA CGGCGCAGAT TGCGCCGCCG TCCTCCGCCT GCCCGGGCGC GTACCCGCTT 
GCGAGCCTGG ACCACCAGCA GATGCACTAC TGCACCTGGC CGTGGAGGGA GCTCCCAACC 
CCCTACACAT GGGAACCCAT CACCGGTGTG GGGTGCCCCT CCAACATGTG CTGCGGCACG 
CCCGTGTACG CATTAGGCGT GTACGTCCCC TACAACTACT TCCTGCCAAT CTGTTCCGCA 
GACGTCACCG GTTGCATTCG GCGCTGCCAA GTGCAGGCGG GTCTGTGCGG TGTGGTGCCT 
GATCGGCCCG TGCTGCACTT TGCGGTGTCA TCGTCCGCGG CTGCGGGCAG CAATAATGCC 
ACAACGCCGG GTGTGGCGGC AGCACCCGTG TCGGCGCCGG TGCCACCTCA GCAGTCGTAC 
ATTGTGTATC CGGGCACGCA CGTGCCGTAT GACGCCGCGG CCGAGATGTG CCGCAGCAGC 
AACTACATGG GACTGGCCTG GAGACTGGTG GCGGCCAGGG AGGTCTTGAC TTGGACAACT 
GCTCTGGACT TGCGCAACCC AGCTTACGGA GCACTGGTGG AGCTGGGTTA CTTCTGGCTG 
GGCGGGGTCG CCGAGGCCAC AGACAGCAGC GGCGGGTCAG CCTGCGCGAC AGCAGTGTAC 
GGCAGTGCAG GGGTGGGCGA CAGCGACGGC AGCGCCACTC CCGACTACGA CCGGGTGACG 
TACGACAGCT CAGTGCCGTG CGACGGCGTG GGGGCGGTGG TGTGCGCGCT TGCGGCGGCG 
CCGGCTGACC CTGAGGGCGG CGGCAACTAC GGCAACATTT CGTGTAGCGC CACGCTGCTT 
GAGACCGCCA ACAGCAGCAC CAGCTCCCAG CAGCAGGAAC AGCCCGCTGC GCTTAACACC 
AATACGACTA CTGTGGTCCT GGCGGCGGCG CCACGTGCAT TCCTCGTCAG CACCGCCGTG 
CAAGTGTCAG AGATGGGCAA CTCTGTCAGT GCTGTGTCAG CCCTGGGCGT GGAGCTAGGC 
GGAGGCGGCT CCGCTGCCAC CGATGCCAAC ATGACTGCTG GCAACCCCAG CACACGGGGC 
TGGCACCGGC TAGAGCTGCA GCCTGGGGAG GGGGTGGTGG CGGCGAGCGG CTGTGCGGGA 
GGTCACCTGG AGGTGCTCCT GCTGCACACC AGCTCGGGCC GCGTGTTGAT GCCGCCGCTG 
ACGGCGGGGC CGGAGGATGC CGCCACCGGC TCGGCGGCGT GCTCTTCCTC GTTTGTGGAG 
ACGGCCCCGG CCGGCGCCTA CTTGGTCGGC TTGCAGGCGA AGCAGTCGAC TGAAGGCAAG 
CTGCATAGTG ATGGCAGCGT GGGACCCGCA GCCACCGGGA CGCCCAAGGG TGGCGGCGGT 
GGTGGCGGCT ACAGCGATCC TGCTGGAGCC GCTTCTCCCC TGTCTTCAGA TGGGTGTGCC 
ATGGCCAGCG GCAGTGCCAG CAGCATTGCC AACAACAGCG CCAGCGGCAG TACCCTCAAC 
GCGCATGCCC GCACCGCCTC ACGTCGCCGG TGCAACCAGC CACCCCTGGG GCCGCAGGGG 
CTGCCAGCGA TAGCGGAAGA GCTGGACTTC CACAAGCGCA TGTGTGCGTA CGTCAGCTCG 
TGCCATGTGT TGGCAAGCGG GGCGGGCAAG TGCTCTTCGG TGGTTGATGA TGCGGGTGCG 
CCTGTTGGGG CGGCTCAGCA GCAGGAGGCG GAAGGAGGCG GTGCGCTGGG CGCTCAGCCG 
GCACAAGAGC CGCCAGCCAC TGCTGGCGCC GCCCTTGGCG ATGCTGGCAA CGGCGGGCCT 
CAGGGCATTG AGCAGTCGCT GCTGCATGCC CAGGCTCAGC TGGCGGGGCG CGCACAGGAG 
CAGCAAGGCG CGCAGCGGGG GCAGCGTCGT GACCTGGTCC TGACGTCGGT CCTGGGTAGC 
GGCAGCTTTG GTGTGGTGTT CAACGGCACC TGGCGCGGGC TGCGGGTGGC AGTCAAGACG 
CTGGTGGTGC ATGACTCGCT GCTGGGGACA GAGGCCCGGC GGCGCCACCG CGCCGTCTTG 
GAAGCTGCCA TCTCCAAGTC TCTCAACCAC CCGAACGTGG TCGCCACCTA TGAGACAGAG 
GTCATTCCTC TGTCCGTGCC CGGCACTGCG AGGAACCTGT CGGCACTCCC GGACCCGTTA 
GCAGCCGGTG GAGACCCTGC AGGCAATGCC TCGGAAGCGG TCGTGCTGGA TGCGACAACG 
GACACATACA AACTCCTACT CGTTATGGAG TATTGCGACG CAGGCTCGCT GACGCGCGCA 
CTGGAGATGG GCGCAGTGGG CAGCGTCGCG GCAGGCGGCG CCGCCGGGCT GTGTGCGCTG 
ACTCTGGCCC TGGATGTGGC GTGCGGCATG CGTCACATAC ACGCCCGCAA CATCGTGCAT 
GGCGACCTGT CGGCAGGGAA CATCCTCCTC TGCAGCACCG CGGACGCCCA GTGCCGCGGC 
GGTTGTGACC TGGCCGATGC ACCGCTGCGC TCGCCGCTGG CTGGGCTGTG GGCGCCGCCC 
CTGGTGGCCA AGGTGGCGGA CTTCGGGCTG AGCGTGCACA TGGGCGAGCA CCAGACACAC 
GCATCCAACA AATTCCAGGG CACGCCCGCC TACACCGCGC CTGAGGTCCT GTCCCGCGGC 
CGCCTGTCCT CCGCCGCTGA CGTCTGGTCC TTTGGCTGCA TCCTGCTTGA GCTCACTCAC 
GGAGAGCGCA TCAGCCGCAT CCGGGCACGG GCGGCCACCG CCGCAGCTGC GGCTCCTGCA 
GCTGCGGCCC CCGCACCAGC AGCAGCCGGT CCAGGCGATC TGCATGGCAC CAGTGGGAAG 
CCGGTGTGGG TGCCATGGAC TGCGCCGCCG TCCGGCAGCT CGCCACGTCT GGCGCAGCTG 
CTGGCCGCCT GCCTGGCCAT GGAACCCAGT GCCCGGCCTT CGTTTGACCA GGTGGCAGAG 
GCGCTGGTCG CGATCCTGGT GGAGTCCCAC CGCAATGCCC AGCACAATGT GCTGACTACC 
ATGAGAGTGA GCAGTACACC ACCGCCGCAA AGCCCTGTGA GCGAATCTGC TGGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001694593.1
CDS	1..4380
Translation	MNSGTTVGGSSVKPVNSIRTAARPGSRRLLLLLFLIASNGKQSELVLAADVQDIWSGLSGTEPSSQGERGAAPSRGGLWTWDESRLCLAALLTQQPTAAAAAGAAVGDAATPAPVLVSLEGRPCTQRLSYACVPDADVRAAGLTPSSEWFRAYVRNGSTAGAAAGATAATAALVVGSSSPHLLGPERSGLVLLRSGFALGGTHVPLGLKAEEWGPQLMNNSMQGAFGTEPYYSIEAPAQLYTLAFDDSDRFWSRGPVVSAFTRSYNGRLLALRPVREAEVDAAISTDTSAAAAGNTSSDSDSLLREGVFGASRPDLDGISSAPYETHHVGSYNDYVVTVQGCFRTLAVEVLLLVTRSGRRYRLGRGSCTVWFREDAPPGGYLAGFAGLQLDVGYWSSQPPMRWAEGQVPNAPWLMQLELVWAAPAGSQPPAYTQRLPALLRHSTAQIAPPSSACPGAYPLASLDHQQMHYCTWPWRELPTPYTWEPITGVGCPSNMCCGTPVYALGVYVPYNYFLPICSADVTGCIRRCQVQAGLCGVVPDRPVLHFAVSSSAAAGSNNATTPGVAAAPVSAPVPPQQSYIVYPGTHVPYDAAAEMCRSSNYMGLAWRLVAAREVLTWTTALDLRNPAYGALVELGYFWLGGVAEATDSSGGSACATAVYGSAGVGDSDGSATPDYDRVTYDSSVPCDGVGAVVCALAAAPADPEGGGNYGNISCSATLLETANSSTSSQQQEQPAALNTNTTTVVLAAAPRAFLVSTAVQVSEMGNSVSAVSALGVELGGGGSAATDANMTAGNPSTRGWHRLELQPGEGVVAASGCAGGHLEVLLLHTSSGRVLMPPLTAGPEDAATGSAACSSSFVETAPAGAYLVGLQAKQSTEGKLHSDGSVGPAATGTPKGGGGGGGYSDPAGAASPLSSDGCAMASGSASSIANNSASGSTLNAHARTASRRRCNQPPLGPQGLPAIAEELDFHKRMCAYVSSCHVLASGAGKCSSVVDDAGAPVGAAQQQEAEGGGALGAQPAQEPPATAGAALGDAGNGGPQGIEQSLLHAQAQLAGRAQEQQGAQRGQRRDLVLTSVLGSGSFGVVFNGTWRGLRVAVKTLVVHDSLLGTEARRRHRAVLEAAISKSLNHPNVVATYETEVIPLSVPGTARNLSALPDPLAAGGDPAGNASEAVVLDATTDTYKLLLVMEYCDAGSLTRALEMGAVGSVAAGGAAGLCALTLALDVACGMRHIHARNIVHGDLSAGNILLCSTADAQCRGGCDLADAPLRSPLAGLWAPPLVAKVADFGLSVHMGEHQTHASNKFQGTPAYTAPEVLSRGRLSSAADVWSFGCILLELTHGERISRIRARAATAAAAAPAAAAPAPAAAGPGDLHGTSGKPVWVPWTAPPSGSSPRLAQLLAACLAMEPSARPSFDQVAEALVAILVESHRNAQHNVLTTMRVSSTPPPQSPVSESAGQ

Target ORF information:

RefSeq Version	XM_001694541.1
Organism	Chlamydomonas reinhardtii
Definition	Chlamydomonas reinhardtii mitogen activated protein kinase kinase kinase 2 (MAPKKK2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001694541.1

ORF Insert Sequence:

ATGAATTCTG GCACCACCGT TGGCGGGAGC AGCGTCAAGC CTGTCAACTC GATAAGGACC 
GCTGCGCGGC CCGGGTCGAG GCGCTTGCTA CTGCTGTTGT TTCTGATTGC ATCCAACGGG 
AAGCAATCTG AGCTGGTGCT CGCTGCAGAC GTGCAGGATA TCTGGTCCGG ACTTTCAGGC 
ACGGAGCCAT CGTCCCAAGG AGAGCGCGGG GCCGCGCCGT CCCGTGGCGG GCTGTGGACG 
TGGGATGAAT CTCGGCTGTG CCTTGCGGCG CTGCTGACCC AGCAACCAAC GGCTGCGGCT 
GCGGCGGGGG CAGCTGTGGG GGACGCGGCC ACCCCGGCAC CTGTGCTCGT GAGCCTGGAG 
GGGCGGCCGT GCACCCAGCG CCTGTCGTAC GCGTGCGTGC CAGATGCCGA CGTTCGAGCG 
GCGGGCCTGA CGCCCTCCTC AGAGTGGTTC CGTGCATATG TCCGGAATGG CTCCACGGCT 
GGTGCGGCTG CGGGTGCTAC AGCCGCTACC GCTGCGCTGG TCGTCGGCAG CAGCAGCCCC 
CATCTGTTGG GGCCTGAGCG CAGTGGGCTG GTGCTGCTGC GCTCGGGTTT CGCCTTGGGA 
GGCACGCACG TGCCGCTGGG GCTCAAAGCG GAGGAGTGGG GTCCGCAGCT TATGAACAAC 
TCTATGCAGG GGGCATTTGG AACGGAACCT TACTACAGTA TTGAAGCTCC AGCGCAGCTG 
TACACATTGG CGTTCGATGA CTCGGATCGG TTTTGGAGCC GCGGTCCCGT GGTCTCAGCC 
TTTACAAGGT CGTATAATGG GAGGCTGTTG GCGTTGCGGC CGGTGCGAGA GGCCGAGGTT 
GATGCAGCCA TTTCTACTGA CACGTCGGCA GCAGCTGCTG GGAACACCAG CAGTGACAGT 
GACAGCCTCC TCCGAGAAGG CGTGTTCGGC GCAAGCCGGC CTGATCTGGA TGGCATCAGC 
AGTGCGCCGT ACGAGACGCA TCACGTGGGG TCGTACAATG ACTACGTGGT GACTGTGCAG 
GGCTGCTTTC GCACGCTGGC CGTGGAGGTG CTTCTGCTGG TCACTCGCAG CGGGCGGCGC 
TACCGGCTGG GCCGCGGCAG CTGCACCGTG TGGTTCAGGG AGGACGCGCC GCCCGGTGGC 
TACCTGGCTG GCTTTGCGGG CCTTCAGCTG GACGTGGGAT ATTGGAGCAG CCAGCCCCCG 
ATGCGTTGGG CCGAAGGCCA GGTGCCCAAC GCACCGTGGC TCATGCAGCT GGAGCTGGTG 
TGGGCCGCGC CTGCCGGATC GCAACCTCCC GCATACACAC AGCGGCTGCC GGCCCTGCTG 
CGGCACTCCA CGGCGCAGAT TGCGCCGCCG TCCTCCGCCT GCCCGGGCGC GTACCCGCTT 
GCGAGCCTGG ACCACCAGCA GATGCACTAC TGCACCTGGC CGTGGAGGGA GCTCCCAACC 
CCCTACACAT GGGAACCCAT CACCGGTGTG GGGTGCCCCT CCAACATGTG CTGCGGCACG 
CCCGTGTACG CATTAGGCGT GTACGTCCCC TACAACTACT TCCTGCCAAT CTGTTCCGCA 
GACGTCACCG GTTGCATTCG GCGCTGCCAA GTGCAGGCGG GTCTGTGCGG TGTGGTGCCT 
GATCGGCCCG TGCTGCACTT TGCGGTGTCA TCGTCCGCGG CTGCGGGCAG CAATAATGCC 
ACAACGCCGG GTGTGGCGGC AGCACCCGTG TCGGCGCCGG TGCCACCTCA GCAGTCGTAC 
ATTGTGTATC CGGGCACGCA CGTGCCGTAT GACGCCGCGG CCGAGATGTG CCGCAGCAGC 
AACTACATGG GACTGGCCTG GAGACTGGTG GCGGCCAGGG AGGTCTTGAC TTGGACAACT 
GCTCTGGACT TGCGCAACCC AGCTTACGGA GCACTGGTGG AGCTGGGTTA CTTCTGGCTG 
GGCGGGGTCG CCGAGGCCAC AGACAGCAGC GGCGGGTCAG CCTGCGCGAC AGCAGTGTAC 
GGCAGTGCAG GGGTGGGCGA CAGCGACGGC AGCGCCACTC CCGACTACGA CCGGGTGACG 
TACGACAGCT CAGTGCCGTG CGACGGCGTG GGGGCGGTGG TGTGCGCGCT TGCGGCGGCG 
CCGGCTGACC CTGAGGGCGG CGGCAACTAC GGCAACATTT CGTGTAGCGC CACGCTGCTT 
GAGACCGCCA ACAGCAGCAC CAGCTCCCAG CAGCAGGAAC AGCCCGCTGC GCTTAACACC 
AATACGACTA CTGTGGTCCT GGCGGCGGCG CCACGTGCAT TCCTCGTCAG CACCGCCGTG 
CAAGTGTCAG AGATGGGCAA CTCTGTCAGT GCTGTGTCAG CCCTGGGCGT GGAGCTAGGC 
GGAGGCGGCT CCGCTGCCAC CGATGCCAAC ATGACTGCTG GCAACCCCAG CACACGGGGC 
TGGCACCGGC TAGAGCTGCA GCCTGGGGAG GGGGTGGTGG CGGCGAGCGG CTGTGCGGGA 
GGTCACCTGG AGGTGCTCCT GCTGCACACC AGCTCGGGCC GCGTGTTGAT GCCGCCGCTG 
ACGGCGGGGC CGGAGGATGC CGCCACCGGC TCGGCGGCGT GCTCTTCCTC GTTTGTGGAG 
ACGGCCCCGG CCGGCGCCTA CTTGGTCGGC TTGCAGGCGA AGCAGTCGAC TGAAGGCAAG 
CTGCATAGTG ATGGCAGCGT GGGACCCGCA GCCACCGGGA CGCCCAAGGG TGGCGGCGGT 
GGTGGCGGCT ACAGCGATCC TGCTGGAGCC GCTTCTCCCC TGTCTTCAGA TGGGTGTGCC 
ATGGCCAGCG GCAGTGCCAG CAGCATTGCC AACAACAGCG CCAGCGGCAG TACCCTCAAC 
GCGCATGCCC GCACCGCCTC ACGTCGCCGG TGCAACCAGC CACCCCTGGG GCCGCAGGGG 
CTGCCAGCGA TAGCGGAAGA GCTGGACTTC CACAAGCGCA TGTGTGCGTA CGTCAGCTCG 
TGCCATGTGT TGGCAAGCGG GGCGGGCAAG TGCTCTTCGG TGGTTGATGA TGCGGGTGCG 
CCTGTTGGGG CGGCTCAGCA GCAGGAGGCG GAAGGAGGCG GTGCGCTGGG CGCTCAGCCG 
GCACAAGAGC CGCCAGCCAC TGCTGGCGCC GCCCTTGGCG ATGCTGGCAA CGGCGGGCCT 
CAGGGCATTG AGCAGTCGCT GCTGCATGCC CAGGCTCAGC TGGCGGGGCG CGCACAGGAG 
CAGCAAGGCG CGCAGCGGGG GCAGCGTCGT GACCTGGTCC TGACGTCGGT CCTGGGTAGC 
GGCAGCTTTG GTGTGGTGTT CAACGGCACC TGGCGCGGGC TGCGGGTGGC AGTCAAGACG 
CTGGTGGTGC ATGACTCGCT GCTGGGGACA GAGGCCCGGC GGCGCCACCG CGCCGTCTTG 
GAAGCTGCCA TCTCCAAGTC TCTCAACCAC CCGAACGTGG TCGCCACCTA TGAGACAGAG 
GTCATTCCTC TGTCCGTGCC CGGCACTGCG AGGAACCTGT CGGCACTCCC GGACCCGTTA 
GCAGCCGGTG GAGACCCTGC AGGCAATGCC TCGGAAGCGG TCGTGCTGGA TGCGACAACG 
GACACATACA AACTCCTACT CGTTATGGAG TATTGCGACG CAGGCTCGCT GACGCGCGCA 
CTGGAGATGG GCGCAGTGGG CAGCGTCGCG GCAGGCGGCG CCGCCGGGCT GTGTGCGCTG 
ACTCTGGCCC TGGATGTGGC GTGCGGCATG CGTCACATAC ACGCCCGCAA CATCGTGCAT 
GGCGACCTGT CGGCAGGGAA CATCCTCCTC TGCAGCACCG CGGACGCCCA GTGCCGCGGC 
GGTTGTGACC TGGCCGATGC ACCGCTGCGC TCGCCGCTGG CTGGGCTGTG GGCGCCGCCC 
CTGGTGGCCA AGGTGGCGGA CTTCGGGCTG AGCGTGCACA TGGGCGAGCA CCAGACACAC 
GCATCCAACA AATTCCAGGG CACGCCCGCC TACACCGCGC CTGAGGTCCT GTCCCGCGGC 
CGCCTGTCCT CCGCCGCTGA CGTCTGGTCC TTTGGCTGCA TCCTGCTTGA GCTCACTCAC 
GGAGAGCGCA TCAGCCGCAT CCGGGCACGG GCGGCCACCG CCGCAGCTGC GGCTCCTGCA 
GCTGCGGCCC CCGCACCAGC AGCAGCCGGT CCAGGCGATC TGCATGGCAC CAGTGGGAAG 
CCGGTGTGGG TGCCATGGAC TGCGCCGCCG TCCGGCAGCT CGCCACGTCT GGCGCAGCTG 
CTGGCCGCCT GCCTGGCCAT GGAACCCAGT GCCCGGCCTT CGTTTGACCA GGTGGCAGAG 
GCGCTGGTCG CGATCCTGGT GGAGTCCCAC CGCAATGCCC AGCACAATGT GCTGACTACC 
ATGAGAGTGA GCAGTACACC ACCGCCGCAA AGCCCTGTGA GCGAATCTGC TGGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Science (New York, N.Y.)318(5848)245-50(2007 Oct)
Merchant SS,Prochnik SE,Vallon O,Harris EH,Karpowicz SJ,Witman GB,Terry A,Salamov A,Fritz-Laylin LK,Maréchal-Drouard L,Marshall WF,Qu LH,Nelson DR,Sanderfoot AA,Spalding MH,Kapitonov VV,Ren Q,Ferris P,Lindquist E,Shapiro H,Lucas SM,Grimwood J,Schmutz J,Cardol P,Cerutti H,Chanfreau G,Chen CL,Cognat V,Croft MT,Dent R,Dutcher S,Fernández E,Fukuzawa H,González-Ballester D,González-Halphen D,Hallmann A,Hanikenne M,Hippler M,Inwood W,Jabbari K,Kalanon M,Kuras R,Lefebvre PA,Lemaire SD,Lobanov AV,Lohr M,Manuell A,Meier I,Mets L,Mittag M,Mittelmeier T,Moroney JV,Moseley J,Napoli C,Nedelcu AM,Niyogi K,Novoselov SV,Paulsen IT,Pazour G,Purton S,Ral JP,Riaño-Pachón DM,Riekhof W,Rymarquis L,Schroda M,Stern D,Umen J,Willows R,Wilson N,Zimmer SL,Allmer J,Balk J,Bisova K,Chen CJ,Elias M,Gendler K,Hauser C,Lamb MR,Ledford H,Long JC,Minagawa J,Page MD,Pan J,Pootakham W,Roje S,Rose A,Stahlberg E,Terauchi AM,Yang P,Ball S,Bowler C,Dieckmann CL,Gladyshev VN,Green P,Jorgensen R,Mayfield S,Mueller-Roeber B,Rajamani S,Sayre RT,Brokstein P,Dubchak I,Goodstein D,Hornick L,Huang YW,Jhaveri J,Luo Y,Martínez D,Ngau WC,Otillar B,Poliakov A,Porter A,Szajkowski L,Werner G,Zhou K,Grigoriev IV,Rokhsar DS,Grossman AR

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MAPKKK2 cDNA ORF clone, Chlamydomonas reinhardtii

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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