RPA12 cDNA ORF clone, Chlamydomonas reinhardtii

The following RPA12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPA12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCh00192 XM_001689881.1
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Chlamydomonas reinhardtii DNA-directed RNA polymerase I subunit (RPA12), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCh00192
    Clone ID Related Accession (Same CDS sequence) XM_001689881.1
    Accession Version XM_001689881.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1503849600000
    Organism Chlamydomonas reinhardtii
    Product DNA-directed RNA polymerase I subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843471).

    1
    61
    121
    181
    241
    301
    361
    ATGTCGTCGG ACAAGGACTG GATGTTCTGC CCCACGTCGG GGTACCTGCT GCACCTCGAC 
    CCCAAGCGGC AAGTGGCCGC TTGCCCCGTG TCGGGCTACG AGCGCAGCCT GGCGGAGTTA
    TCATCCGTGA AAGTGGTTCT ACGCACGGAC ATGGAGGACT ACCGGCGCCG TTTTGCGCTT
    GAGCCGCTGG TGAAGAGCGT GGAGGATGAG GAGCTGCTGA AGGGCCGCAA GCGCGCCACG
    GTGGACGAGC CCTGCCCCAA GTGCAACCAC CGTCAGCTGG AGTACTACAC CATGCAGCTG
    CGCTCGGCGG ATGAGGGCCA GACCGTCTTC TACGAGTGCA AGAACTGCGG GCATAAGTAT
    TCTCAGAACA CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001689933.1
    CDS266..640
    Translation

    Target ORF information:

    RefSeq Version XM_001689881.1
    Organism Chlamydomonas reinhardtii
    Definition Chlamydomonas reinhardtii DNA-directed RNA polymerase I subunit (RPA12), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001689881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGTCGTCGG ACAAGGACTG GATGTTCTGC CCCACGTCGG GGTACCTGCT GCACCTCGAC 
    CCCAAGCGGC AAGTGGCCGC TTGCCCCGTG TCGGGCTACG AGCGCAGCCT GGCGGAGTTA
    TCATCCGTGA AAGTGGTTCT ACGCACGGAC ATGGAGGACT ACCGGCGCCG TTTTGCGCTT
    GAGCCGCTGG TGAAGAGCGT GGAGGATGAG GAGCTGCTGA AGGGCCGCAA GCGCGCCACG
    GTGGACGAGC CCTGCCCCAA GTGCAACCAC CGTCAGCTGG AGTACTACAC CATGCAGCTG
    CGCTCGGCGG ATGAGGGCCA GACCGTCTTC TACGAGTGCA AGAACTGCGG GCATAAGTAT
    TCTCAGAACA CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Chlamydomonas genome reveals the evolution of key animal and plant functions.
    Science (New York, N.Y.)318(5848)245-50(2007 Oct)
    Merchant SS,Prochnik SE,Vallon O,Harris EH,Karpowicz SJ,Witman GB,Terry A,Salamov A,Fritz-Laylin LK,Maréchal-Drouard L,Marshall WF,Qu LH,Nelson DR,Sanderfoot AA,Spalding MH,Kapitonov VV,Ren Q,Ferris P,Lindquist E,Shapiro H,Lucas SM,Grimwood J,Schmutz J,Cardol P,Cerutti H,Chanfreau G,Chen CL,Cognat V,Croft MT,Dent R,Dutcher S,Fernández E,Fukuzawa H,González-Ballester D,González-Halphen D,Hallmann A,Hanikenne M,Hippler M,Inwood W,Jabbari K,Kalanon M,Kuras R,Lefebvre PA,Lemaire SD,Lobanov AV,Lohr M,Manuell A,Meier I,Mets L,Mittag M,Mittelmeier T,Moroney JV,Moseley J,Napoli C,Nedelcu AM,Niyogi K,Novoselov SV,Paulsen IT,Pazour G,Purton S,Ral JP,Riaño-Pachón DM,Riekhof W,Rymarquis L,Schroda M,Stern D,Umen J,Willows R,Wilson N,Zimmer SL,Allmer J,Balk J,Bisova K,Chen CJ,Elias M,Gendler K,Hauser C,Lamb MR,Ledford H,Long JC,Minagawa J,Page MD,Pan J,Pootakham W,Roje S,Rose A,Stahlberg E,Terauchi AM,Yang P,Ball S,Bowler C,Dieckmann CL,Gladyshev VN,Green P,Jorgensen R,Mayfield S,Mueller-Roeber B,Rajamani S,Sayre RT,Brokstein P,Dubchak I,Goodstein D,Hornick L,Huang YW,Jhaveri J,Luo Y,Martínez D,Ngau WC,Otillar B,Poliakov A,Porter A,Szajkowski L,Werner G,Zhou K,Grigoriev IV,Rokhsar DS,Grossman AR