Alpk1 cDNA ORF clone, Cavia porcellus(domestic guinea pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alpk1
Entrez Gene ID	100716718
Full Name	alpha kinase 1
Gene Type	protein-coding
Organism	Cavia porcellus(domestic guinea pig)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003468058.2	XP_003468106.1	alpha-protein kinase 1 isoform X1
XM_023564345.1	XP_023420113.1	alpha-protein kinase 1
XM_013150619.1	XP_013006073.1	alpha-protein kinase 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following Alpk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alpk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODo10168	XM_023564345.1 Latest version!	Cavia porcellus alpha kinase 1 (Alpk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODo10168	XM_003468058.2 Latest version!	Cavia porcellus alpha-kinase 1 (Alpk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODo28351	XM_013150619.1 Latest version!	Cavia porcellus alpha-kinase 1 (Alpk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alpk1 ( XM_003468058.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_003468106.1 Cavia porcellus alpha-kinase 1 (Alpk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODo10168
Clone ID Related Accession (Same CDS sequence)	XM_003468058.2 , XM_023564345.1
Accession Version	XM_003468058.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	alpha-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176376.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 14, 2015 this sequence version replaced XM_003468058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC ATAAAGCTGT TGCTGAGCTC CTGCAAGAGT GCGAGCAAGC ACTGGACCAG 
CTCTTGGTGG CGCCACCAGA TGTGTCCGAG GAGGACAAGA GGGAGGACCA GCGATGCCGA 
GCTTCACTCT CCAGAGAGTT AAGAACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCCTGAGCC CAGAGGACAA AACCAACCTG 
CAGGATGTGA TTGGCGCTGC GCTGCACCAG TTGCTGGTGG CCCTGCGTGC CTCCATCCGG 
GCCCAGGACT GCACAGTGGC CACAGCCGTT ATCTTCCTGC TGGACCGTGT GCTCTACAGG 
CTGGACGGCA CAGGCAGCCT CCTGCGGGTG GCACGGCGCC TGCACCGCCT GCAGCCCAGC 
ACACCCATCG CACCACAGCT GCTTATCCGA CAGGCGCGTG TCTCGGTTAA CGCAGGGAAG 
CTCCTGAAAG CAGAGTACAT CCTCAGCAGC CTCATCAGCA ACCACGGAGC CACAGGTACC 
TGGCTCTACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGTGT ACAGATCCGA 
GGGCAAATTC TGCAAAAGCT TGGCATGTGG TATGAAGCCG CCGAGTTAAC AAGTGCCTCC 
ATCGTTGGAT ATTTGACGCT CCCTCTGCCG GATAAAAAGG GCCTCTCTGC GTCACTTGGG 
ATTCTGGCAG ACATCTTTGT TTCCATGAGT GAGAACGACT ATGAGAGATT TAAGAACAAC 
CCCCAGGTTA ACTTGGCCCT GCTCCAGGAG TATGAACACC GGCTACTGGC AGCAGCAGAA 
GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TACACACCCC TGTTTGTGCT CACAGCCATG 
AACATCCGCG GCATGTGCTT GCTGTCCTAC AGCAGTTCCA GTGCCTGCCC TGTCAACCAG 
ACCAGCTCCT ACCTGTGTGC GGCTAAAGAG GCCTTCGAGA TCGGCCTGCT GACCAAGACT 
GACAACACGC CGGCCGCCGG GAAGCAGGAG CTCCACAGCC TCATCAAGGC AGCCTTCGGC 
CTGGCCACTG TGCACCGCCG ACTCCTCGGG AACACGGCCG CGGTGCGTGT GGCCCGCTGG 
CTGTGTGTGG AGGCCCTGGG GACACTGGCC AGGCTGGGCT CCTGCTCTGC AAGCCAGGAC 
CCAGAGGCAG TGTCAGGAGA GATCCTGTCT GCAATCAGCC AAGTGAGGCG GTGCCTCGGT 
GTCCAGAGCT TTGTAAACCC AGACGCCAGA GCTTGCATTC CGGAGAGTTT CCAGGGTGGC 
TTGGATGAGC CTCTTCTGCG GGGGAGCGTG GGTTTCCAGG AGATCCTCAC CGGCTACTCG 
CGGCACCACA GCTCAGTATG TGAGGTTTTT GAAAGCACAT GTGGACACAG CAAGATCCAC 
GAGAAACACG CAAAAACAGA CCTCTGCATC ACGGCCTTAG AAACAGAAAC CAAATCTCCA 
GACACACACA CCCTTGTGGA CCGGCCACGC TCTCCTGCAG GAAGGGGGAC ACTGTGCTAC 
TGGACAGACC AAATCGAGAG GGCAGGCATT GAGGCCTGGG CCCCACATCG GGACACGGAA 
CCTGGGGCAG AGCCACCCGG GCACCACAGC AGCAAGCTCC AGGAGAGGTC CCCGGGGGGC 
AGCTGGGGTT CTGGGAGCTG GAGCAGGCTG TCGGGGACCA GTTGTTCTGT GAGCTGGGAG 
GAGGTGAGCG CTGCGGGGGT GCCCAGCTCA GCTGGGGGCA CAGAGCACCT CGTGGACAGC 
CGGTGCCACA CTGCCTCATG TGAGGAGCTT GAGCCAGACA TCGAGGGCAG AGCCGGGCAC 
TTGGTGCCTT GGGATCTGCG GGGTCTGTCT CTCCGGGTGC CCAAGGATGA CTTTACAGAG 
ACCTCCCCAA GTCCTCTACA CGGAGCCCCG TCCTCACCAA ACCTCGCACC CCACAGCTCT 
GGCGACACCC AGGCAGTCCC TGTGGCCGAG CTGGCGGGGG CTCCAGGGGA CACCTGCGAA 
GTCAGAGGGA CTCTCTCGGG ACTCCCCCGC GCTTCTCCGG CTCCTGGTTT CGTCGGGCAC 
AGGGACACGG CCGCGTGGCA TTCCGTGCAG AACAACAACT TTGAAATTCT TGATTTCGCA 
GACACCAGCT GTTATGTCCC AGGCGGGCGT GGGGTAGCCA GGAAAACAGA AGCCCATGAT 
CCAGACCCAG GCCGCACGTG GGTGGACCCC GATGCAGAGA CTGCAGAAGG CACCCAGGCA 
GAGCCAGGTG GTTCGCGGGG CGCCAGTGCC AGGCCGGGGA ACAGCTCTCT CCCTCCCTGT 
CGCCCCACCT GCCCTGAGAC CAGGGGCTCC GCGGAGGAGC CAGGAGTCGA CCCCGAGGGC 
CCCACGGAGG TCGAGGGCGC CCCCTCTGCG GCTGGACCCC CCGGGCTCCC TTCGGGTGGC 
GGTAGCACCT TCCCTGTCCC CAGCGAGAGC TGTTCCTCCA CAGAGGAGCC GGGCCTGGGC 
GCCTTCTCCA GCGGCTCCCC GGGCTGCTCC AGCCCCTGGT CCCTGCTGGA CTCCTGCGGC 
GGCTCCGCGG CAGCCCGGCC AGGGCCGGAG CAGGAGGCGC TAGAGGCGCG CACGCTGCGG 
CCCGCGGACC TGCGGGAGCT GCTGGCCGGC GTGGGCCATG GCTGGCTGCG GCGGAGGCTG 
GAGGGCACCG GCGTGTTCAA GCCCGGCCGG CCGCGCGGGG CGCACGATGG TCTTCTGTTA 
AAATATTCCA AAAAGTCTGA ACTGTGGACA GCCCAGGAAA CGGCGGTGTA TCTGGGGGAC 
TACCTGGATG TGAAGAAGAA AGGCAAACAG AGGAACGCCT TCTGGGTTCA CCATCTTCAC 
CAAGAAGAAA CCCTGGGGAG GTACGTTGGG AAGGAATACA AAGAACAGAA GGGGCTCTGG 
CATCACTTCG CTGATGTGGA GAGGCAGATG ACCGCACAGC ACTACGTGAC AGAGTTCAAC 
AAGAAACTCT ATGAGCAAAA TATCCCAACA CAGATATTCT ACATCCCGTC CACAGTGCTG 
CTGATTTTAG AAGGCAACAC AATAACGGGA TGTATCAGTG TGGAGCCTTA CATACCAGGA 
GAGTTTGTAA AATTATCAAA CAACACAAAA GTGGTGAAAA CAGAGTACAA AGCCACAGAA 
TACGGCTTGG CTTATGGCCA TTTTTCTTAT GAGTTTTCTA ATCATAGAGA TGTTGTGGTT 
GACTTACAAG GCTGGGTGAC TGGTAATGGC AAAGGGTTAA TTTACCTCAC AGATCCCCAG 
ATCCACTCTG TTGATCAGAA AGACGTCACT ACCAATTTTG GAAAGAGAGG AATCTTTTAC 
TTCTTTAATA ACCAGCATGT GACATGTAAT GACATCTGCC ATCGTCTTTG TCTGACCCGA 
CCTTCAGAAG GAAACCTGAA CGAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003468106.1
CDS	766..4215
Translation	MNNHKAVAELLQECEQALDQLLVAPPDVSEEDKREDQRCRASLSRELRTLIQEAKEMKWPFVPEKWQYKQALSPEDKTNLQDVIGAALHQLLVALRASIRAQDCTVATAVIFLLDRVLYRLDGTGSLLRVARRLHRLQPSTPIAPQLLIRQARVSVNAGKLLKAEYILSSLISNHGATGTWLYRNESDKVLVQSVCVQIRGQILQKLGMWYEAAELTSASIVGYLTLPLPDKKGLSASLGILADIFVSMSENDYERFKNNPQVNLALLQEYEHRLLAAAEACKLAAAFSAYTPLFVLTAMNIRGMCLLSYSSSSACPVNQTSSYLCAAKEAFEIGLLTKTDNTPAAGKQELHSLIKAAFGLATVHRRLLGNTAAVRVARWLCVEALGTLARLGSCSASQDPEAVSGEILSAISQVRRCLGVQSFVNPDARACIPESFQGGLDEPLLRGSVGFQEILTGYSRHHSSVCEVFESTCGHSKIHEKHAKTDLCITALETETKSPDTHTLVDRPRSPAGRGTLCYWTDQIERAGIEAWAPHRDTEPGAEPPGHHSSKLQERSPGGSWGSGSWSRLSGTSCSVSWEEVSAAGVPSSAGGTEHLVDSRCHTASCEELEPDIEGRAGHLVPWDLRGLSLRVPKDDFTETSPSPLHGAPSSPNLAPHSSGDTQAVPVAELAGAPGDTCEVRGTLSGLPRASPAPGFVGHRDTAAWHSVQNNNFEILDFADTSCYVPGGRGVARKTEAHDPDPGRTWVDPDAETAEGTQAEPGGSRGASARPGNSSLPPCRPTCPETRGSAEEPGVDPEGPTEVEGAPSAAGPPGLPSGGGSTFPVPSESCSSTEEPGLGAFSSGSPGCSSPWSLLDSCGGSAAARPGPEQEALEARTLRPADLRELLAGVGHGWLRRRLEGTGVFKPGRPRGAHDGLLLKYSKKSELWTAQETAVYLGDYLDVKKKGKQRNAFWVHHLHQEETLGRYVGKEYKEQKGLWHHFADVERQMTAQHYVTEFNKKLYEQNIPTQIFYIPSTVLLILEGNTITGCISVEPYIPGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRDVVVDLQGWVTGNGKGLIYLTDPQIHSVDQKDVTTNFGKRGIFYFFNNQHVTCNDICHRLCLTRPSEGNLNEP

Target ORF information:

RefSeq Version	XM_003468058.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus alpha-kinase 1 (Alpk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003468058.2

ORF Insert Sequence:

ATGAATAATC ATAAAGCTGT TGCTGAGCTC CTGCAAGAGT GCGAGCAAGC ACTGGACCAG 
CTCTTGGTGG CGCCACCAGA TGTGTCCGAG GAGGACAAGA GGGAGGACCA GCGATGCCGA 
GCTTCACTCT CCAGAGAGTT AAGAACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCCTGAGCC CAGAGGACAA AACCAACCTG 
CAGGATGTGA TTGGCGCTGC GCTGCACCAG TTGCTGGTGG CCCTGCGTGC CTCCATCCGG 
GCCCAGGACT GCACAGTGGC CACAGCCGTT ATCTTCCTGC TGGACCGTGT GCTCTACAGG 
CTGGACGGCA CAGGCAGCCT CCTGCGGGTG GCACGGCGCC TGCACCGCCT GCAGCCCAGC 
ACACCCATCG CACCACAGCT GCTTATCCGA CAGGCGCGTG TCTCGGTTAA CGCAGGGAAG 
CTCCTGAAAG CAGAGTACAT CCTCAGCAGC CTCATCAGCA ACCACGGAGC CACAGGTACC 
TGGCTCTACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGTGT ACAGATCCGA 
GGGCAAATTC TGCAAAAGCT TGGCATGTGG TATGAAGCCG CCGAGTTAAC AAGTGCCTCC 
ATCGTTGGAT ATTTGACGCT CCCTCTGCCG GATAAAAAGG GCCTCTCTGC GTCACTTGGG 
ATTCTGGCAG ACATCTTTGT TTCCATGAGT GAGAACGACT ATGAGAGATT TAAGAACAAC 
CCCCAGGTTA ACTTGGCCCT GCTCCAGGAG TATGAACACC GGCTACTGGC AGCAGCAGAA 
GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TACACACCCC TGTTTGTGCT CACAGCCATG 
AACATCCGCG GCATGTGCTT GCTGTCCTAC AGCAGTTCCA GTGCCTGCCC TGTCAACCAG 
ACCAGCTCCT ACCTGTGTGC GGCTAAAGAG GCCTTCGAGA TCGGCCTGCT GACCAAGACT 
GACAACACGC CGGCCGCCGG GAAGCAGGAG CTCCACAGCC TCATCAAGGC AGCCTTCGGC 
CTGGCCACTG TGCACCGCCG ACTCCTCGGG AACACGGCCG CGGTGCGTGT GGCCCGCTGG 
CTGTGTGTGG AGGCCCTGGG GACACTGGCC AGGCTGGGCT CCTGCTCTGC AAGCCAGGAC 
CCAGAGGCAG TGTCAGGAGA GATCCTGTCT GCAATCAGCC AAGTGAGGCG GTGCCTCGGT 
GTCCAGAGCT TTGTAAACCC AGACGCCAGA GCTTGCATTC CGGAGAGTTT CCAGGGTGGC 
TTGGATGAGC CTCTTCTGCG GGGGAGCGTG GGTTTCCAGG AGATCCTCAC CGGCTACTCG 
CGGCACCACA GCTCAGTATG TGAGGTTTTT GAAAGCACAT GTGGACACAG CAAGATCCAC 
GAGAAACACG CAAAAACAGA CCTCTGCATC ACGGCCTTAG AAACAGAAAC CAAATCTCCA 
GACACACACA CCCTTGTGGA CCGGCCACGC TCTCCTGCAG GAAGGGGGAC ACTGTGCTAC 
TGGACAGACC AAATCGAGAG GGCAGGCATT GAGGCCTGGG CCCCACATCG GGACACGGAA 
CCTGGGGCAG AGCCACCCGG GCACCACAGC AGCAAGCTCC AGGAGAGGTC CCCGGGGGGC 
AGCTGGGGTT CTGGGAGCTG GAGCAGGCTG TCGGGGACCA GTTGTTCTGT GAGCTGGGAG 
GAGGTGAGCG CTGCGGGGGT GCCCAGCTCA GCTGGGGGCA CAGAGCACCT CGTGGACAGC 
CGGTGCCACA CTGCCTCATG TGAGGAGCTT GAGCCAGACA TCGAGGGCAG AGCCGGGCAC 
TTGGTGCCTT GGGATCTGCG GGGTCTGTCT CTCCGGGTGC CCAAGGATGA CTTTACAGAG 
ACCTCCCCAA GTCCTCTACA CGGAGCCCCG TCCTCACCAA ACCTCGCACC CCACAGCTCT 
GGCGACACCC AGGCAGTCCC TGTGGCCGAG CTGGCGGGGG CTCCAGGGGA CACCTGCGAA 
GTCAGAGGGA CTCTCTCGGG ACTCCCCCGC GCTTCTCCGG CTCCTGGTTT CGTCGGGCAC 
AGGGACACGG CCGCGTGGCA TTCCGTGCAG AACAACAACT TTGAAATTCT TGATTTCGCA 
GACACCAGCT GTTATGTCCC AGGCGGGCGT GGGGTAGCCA GGAAAACAGA AGCCCATGAT 
CCAGACCCAG GCCGCACGTG GGTGGACCCC GATGCAGAGA CTGCAGAAGG CACCCAGGCA 
GAGCCAGGTG GTTCGCGGGG CGCCAGTGCC AGGCCGGGGA ACAGCTCTCT CCCTCCCTGT 
CGCCCCACCT GCCCTGAGAC CAGGGGCTCC GCGGAGGAGC CAGGAGTCGA CCCCGAGGGC 
CCCACGGAGG TCGAGGGCGC CCCCTCTGCG GCTGGACCCC CCGGGCTCCC TTCGGGTGGC 
GGTAGCACCT TCCCTGTCCC CAGCGAGAGC TGTTCCTCCA CAGAGGAGCC GGGCCTGGGC 
GCCTTCTCCA GCGGCTCCCC GGGCTGCTCC AGCCCCTGGT CCCTGCTGGA CTCCTGCGGC 
GGCTCCGCGG CAGCCCGGCC AGGGCCGGAG CAGGAGGCGC TAGAGGCGCG CACGCTGCGG 
CCCGCGGACC TGCGGGAGCT GCTGGCCGGC GTGGGCCATG GCTGGCTGCG GCGGAGGCTG 
GAGGGCACCG GCGTGTTCAA GCCCGGCCGG CCGCGCGGGG CGCACGATGG TCTTCTGTTA 
AAATATTCCA AAAAGTCTGA ACTGTGGACA GCCCAGGAAA CGGCGGTGTA TCTGGGGGAC 
TACCTGGATG TGAAGAAGAA AGGCAAACAG AGGAACGCCT TCTGGGTTCA CCATCTTCAC 
CAAGAAGAAA CCCTGGGGAG GTACGTTGGG AAGGAATACA AAGAACAGAA GGGGCTCTGG 
CATCACTTCG CTGATGTGGA GAGGCAGATG ACCGCACAGC ACTACGTGAC AGAGTTCAAC 
AAGAAACTCT ATGAGCAAAA TATCCCAACA CAGATATTCT ACATCCCGTC CACAGTGCTG 
CTGATTTTAG AAGGCAACAC AATAACGGGA TGTATCAGTG TGGAGCCTTA CATACCAGGA 
GAGTTTGTAA AATTATCAAA CAACACAAAA GTGGTGAAAA CAGAGTACAA AGCCACAGAA 
TACGGCTTGG CTTATGGCCA TTTTTCTTAT GAGTTTTCTA ATCATAGAGA TGTTGTGGTT 
GACTTACAAG GCTGGGTGAC TGGTAATGGC AAAGGGTTAA TTTACCTCAC AGATCCCCAG 
ATCCACTCTG TTGATCAGAA AGACGTCACT ACCAATTTTG GAAAGAGAGG AATCTTTTAC 
TTCTTTAATA ACCAGCATGT GACATGTAAT GACATCTGCC ATCGTCTTTG TCTGACCCGA 
CCTTCAGAAG GAAACCTGAA CGAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpk1 ( XM_023564345.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_023420113.1 Cavia porcellus alpha kinase 1 (Alpk1), mRNA.

Price & Availability↑

CloneID	ODo10168
Clone ID Related Accession (Same CDS sequence)	XM_003468058.2 , XM_023564345.1
Accession Version	XM_023564345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	alpha-protein kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176376.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC ATAAAGCTGT TGCTGAGCTC CTGCAAGAGT GCGAGCAAGC ACTGGACCAG 
CTCTTGGTGG CGCCACCAGA TGTGTCCGAG GAGGACAAGA GGGAGGACCA GCGATGCCGA 
GCTTCACTCT CCAGAGAGTT AAGAACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCCTGAGCC CAGAGGACAA AACCAACCTG 
CAGGATGTGA TTGGCGCTGC GCTGCACCAG TTGCTGGTGG CCCTGCGTGC CTCCATCCGG 
GCCCAGGACT GCACAGTGGC CACAGCCGTT ATCTTCCTGC TGGACCGTGT GCTCTACAGG 
CTGGACGGCA CAGGCAGCCT CCTGCGGGTG GCACGGCGCC TGCACCGCCT GCAGCCCAGC 
ACACCCATCG CACCACAGCT GCTTATCCGA CAGGCGCGTG TCTCGGTTAA CGCAGGGAAG 
CTCCTGAAAG CAGAGTACAT CCTCAGCAGC CTCATCAGCA ACCACGGAGC CACAGGTACC 
TGGCTCTACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGTGT ACAGATCCGA 
GGGCAAATTC TGCAAAAGCT TGGCATGTGG TATGAAGCCG CCGAGTTAAC AAGTGCCTCC 
ATCGTTGGAT ATTTGACGCT CCCTCTGCCG GATAAAAAGG GCCTCTCTGC GTCACTTGGG 
ATTCTGGCAG ACATCTTTGT TTCCATGAGT GAGAACGACT ATGAGAGATT TAAGAACAAC 
CCCCAGGTTA ACTTGGCCCT GCTCCAGGAG TATGAACACC GGCTACTGGC AGCAGCAGAA 
GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TACACACCCC TGTTTGTGCT CACAGCCATG 
AACATCCGCG GCATGTGCTT GCTGTCCTAC AGCAGTTCCA GTGCCTGCCC TGTCAACCAG 
ACCAGCTCCT ACCTGTGTGC GGCTAAAGAG GCCTTCGAGA TCGGCCTGCT GACCAAGACT 
GACAACACGC CGGCCGCCGG GAAGCAGGAG CTCCACAGCC TCATCAAGGC AGCCTTCGGC 
CTGGCCACTG TGCACCGCCG ACTCCTCGGG AACACGGCCG CGGTGCGTGT GGCCCGCTGG 
CTGTGTGTGG AGGCCCTGGG GACACTGGCC AGGCTGGGCT CCTGCTCTGC AAGCCAGGAC 
CCAGAGGCAG TGTCAGGAGA GATCCTGTCT GCAATCAGCC AAGTGAGGCG GTGCCTCGGT 
GTCCAGAGCT TTGTAAACCC AGACGCCAGA GCTTGCATTC CGGAGAGTTT CCAGGGTGGC 
TTGGATGAGC CTCTTCTGCG GGGGAGCGTG GGTTTCCAGG AGATCCTCAC CGGCTACTCG 
CGGCACCACA GCTCAGTATG TGAGGTTTTT GAAAGCACAT GTGGACACAG CAAGATCCAC 
GAGAAACACG CAAAAACAGA CCTCTGCATC ACGGCCTTAG AAACAGAAAC CAAATCTCCA 
GACACACACA CCCTTGTGGA CCGGCCACGC TCTCCTGCAG GAAGGGGGAC ACTGTGCTAC 
TGGACAGACC AAATCGAGAG GGCAGGCATT GAGGCCTGGG CCCCACATCG GGACACGGAA 
CCTGGGGCAG AGCCACCCGG GCACCACAGC AGCAAGCTCC AGGAGAGGTC CCCGGGGGGC 
AGCTGGGGTT CTGGGAGCTG GAGCAGGCTG TCGGGGACCA GTTGTTCTGT GAGCTGGGAG 
GAGGTGAGCG CTGCGGGGGT GCCCAGCTCA GCTGGGGGCA CAGAGCACCT CGTGGACAGC 
CGGTGCCACA CTGCCTCATG TGAGGAGCTT GAGCCAGACA TCGAGGGCAG AGCCGGGCAC 
TTGGTGCCTT GGGATCTGCG GGGTCTGTCT CTCCGGGTGC CCAAGGATGA CTTTACAGAG 
ACCTCCCCAA GTCCTCTACA CGGAGCCCCG TCCTCACCAA ACCTCGCACC CCACAGCTCT 
GGCGACACCC AGGCAGTCCC TGTGGCCGAG CTGGCGGGGG CTCCAGGGGA CACCTGCGAA 
GTCAGAGGGA CTCTCTCGGG ACTCCCCCGC GCTTCTCCGG CTCCTGGTTT CGTCGGGCAC 
AGGGACACGG CCGCGTGGCA TTCCGTGCAG AACAACAACT TTGAAATTCT TGATTTCGCA 
GACACCAGCT GTTATGTCCC AGGCGGGCGT GGGGTAGCCA GGAAAACAGA AGCCCATGAT 
CCAGACCCAG GCCGCACGTG GGTGGACCCC GATGCAGAGA CTGCAGAAGG CACCCAGGCA 
GAGCCAGGTG GTTCGCGGGG CGCCAGTGCC AGGCCGGGGA ACAGCTCTCT CCCTCCCTGT 
CGCCCCACCT GCCCTGAGAC CAGGGGCTCC GCGGAGGAGC CAGGAGTCGA CCCCGAGGGC 
CCCACGGAGG TCGAGGGCGC CCCCTCTGCG GCTGGACCCC CCGGGCTCCC TTCGGGTGGC 
GGTAGCACCT TCCCTGTCCC CAGCGAGAGC TGTTCCTCCA CAGAGGAGCC GGGCCTGGGC 
GCCTTCTCCA GCGGCTCCCC GGGCTGCTCC AGCCCCTGGT CCCTGCTGGA CTCCTGCGGC 
GGCTCCGCGG CAGCCCGGCC AGGGCCGGAG CAGGAGGCGC TAGAGGCGCG CACGCTGCGG 
CCCGCGGACC TGCGGGAGCT GCTGGCCGGC GTGGGCCATG GCTGGCTGCG GCGGAGGCTG 
GAGGGCACCG GCGTGTTCAA GCCCGGCCGG CCGCGCGGGG CGCACGATGG TCTTCTGTTA 
AAATATTCCA AAAAGTCTGA ACTGTGGACA GCCCAGGAAA CGGCGGTGTA TCTGGGGGAC 
TACCTGGATG TGAAGAAGAA AGGCAAACAG AGGAACGCCT TCTGGGTTCA CCATCTTCAC 
CAAGAAGAAA CCCTGGGGAG GTACGTTGGG AAGGAATACA AAGAACAGAA GGGGCTCTGG 
CATCACTTCG CTGATGTGGA GAGGCAGATG ACCGCACAGC ACTACGTGAC AGAGTTCAAC 
AAGAAACTCT ATGAGCAAAA TATCCCAACA CAGATATTCT ACATCCCGTC CACAGTGCTG 
CTGATTTTAG AAGGCAACAC AATAACGGGA TGTATCAGTG TGGAGCCTTA CATACCAGGA 
GAGTTTGTAA AATTATCAAA CAACACAAAA GTGGTGAAAA CAGAGTACAA AGCCACAGAA 
TACGGCTTGG CTTATGGCCA TTTTTCTTAT GAGTTTTCTA ATCATAGAGA TGTTGTGGTT 
GACTTACAAG GCTGGGTGAC TGGTAATGGC AAAGGGTTAA TTTACCTCAC AGATCCCCAG 
ATCCACTCTG TTGATCAGAA AGACGTCACT ACCAATTTTG GAAAGAGAGG AATCTTTTAC 
TTCTTTAATA ACCAGCATGT GACATGTAAT GACATCTGCC ATCGTCTTTG TCTGACCCGA 
CCTTCAGAAG GAAACCTGAA CGAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023420113.1
CDS	669..4118
Translation	MNNHKAVAELLQECEQALDQLLVAPPDVSEEDKREDQRCRASLSRELRTLIQEAKEMKWPFVPEKWQYKQALSPEDKTNLQDVIGAALHQLLVALRASIRAQDCTVATAVIFLLDRVLYRLDGTGSLLRVARRLHRLQPSTPIAPQLLIRQARVSVNAGKLLKAEYILSSLISNHGATGTWLYRNESDKVLVQSVCVQIRGQILQKLGMWYEAAELTSASIVGYLTLPLPDKKGLSASLGILADIFVSMSENDYERFKNNPQVNLALLQEYEHRLLAAAEACKLAAAFSAYTPLFVLTAMNIRGMCLLSYSSSSACPVNQTSSYLCAAKEAFEIGLLTKTDNTPAAGKQELHSLIKAAFGLATVHRRLLGNTAAVRVARWLCVEALGTLARLGSCSASQDPEAVSGEILSAISQVRRCLGVQSFVNPDARACIPESFQGGLDEPLLRGSVGFQEILTGYSRHHSSVCEVFESTCGHSKIHEKHAKTDLCITALETETKSPDTHTLVDRPRSPAGRGTLCYWTDQIERAGIEAWAPHRDTEPGAEPPGHHSSKLQERSPGGSWGSGSWSRLSGTSCSVSWEEVSAAGVPSSAGGTEHLVDSRCHTASCEELEPDIEGRAGHLVPWDLRGLSLRVPKDDFTETSPSPLHGAPSSPNLAPHSSGDTQAVPVAELAGAPGDTCEVRGTLSGLPRASPAPGFVGHRDTAAWHSVQNNNFEILDFADTSCYVPGGRGVARKTEAHDPDPGRTWVDPDAETAEGTQAEPGGSRGASARPGNSSLPPCRPTCPETRGSAEEPGVDPEGPTEVEGAPSAAGPPGLPSGGGSTFPVPSESCSSTEEPGLGAFSSGSPGCSSPWSLLDSCGGSAAARPGPEQEALEARTLRPADLRELLAGVGHGWLRRRLEGTGVFKPGRPRGAHDGLLLKYSKKSELWTAQETAVYLGDYLDVKKKGKQRNAFWVHHLHQEETLGRYVGKEYKEQKGLWHHFADVERQMTAQHYVTEFNKKLYEQNIPTQIFYIPSTVLLILEGNTITGCISVEPYIPGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRDVVVDLQGWVTGNGKGLIYLTDPQIHSVDQKDVTTNFGKRGIFYFFNNQHVTCNDICHRLCLTRPSEGNLNEP

Target ORF information:

RefSeq Version	XM_023564345.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus alpha kinase 1 (Alpk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023564345.1

ORF Insert Sequence:

ATGAATAATC ATAAAGCTGT TGCTGAGCTC CTGCAAGAGT GCGAGCAAGC ACTGGACCAG 
CTCTTGGTGG CGCCACCAGA TGTGTCCGAG GAGGACAAGA GGGAGGACCA GCGATGCCGA 
GCTTCACTCT CCAGAGAGTT AAGAACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCCTGAGCC CAGAGGACAA AACCAACCTG 
CAGGATGTGA TTGGCGCTGC GCTGCACCAG TTGCTGGTGG CCCTGCGTGC CTCCATCCGG 
GCCCAGGACT GCACAGTGGC CACAGCCGTT ATCTTCCTGC TGGACCGTGT GCTCTACAGG 
CTGGACGGCA CAGGCAGCCT CCTGCGGGTG GCACGGCGCC TGCACCGCCT GCAGCCCAGC 
ACACCCATCG CACCACAGCT GCTTATCCGA CAGGCGCGTG TCTCGGTTAA CGCAGGGAAG 
CTCCTGAAAG CAGAGTACAT CCTCAGCAGC CTCATCAGCA ACCACGGAGC CACAGGTACC 
TGGCTCTACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGTGT ACAGATCCGA 
GGGCAAATTC TGCAAAAGCT TGGCATGTGG TATGAAGCCG CCGAGTTAAC AAGTGCCTCC 
ATCGTTGGAT ATTTGACGCT CCCTCTGCCG GATAAAAAGG GCCTCTCTGC GTCACTTGGG 
ATTCTGGCAG ACATCTTTGT TTCCATGAGT GAGAACGACT ATGAGAGATT TAAGAACAAC 
CCCCAGGTTA ACTTGGCCCT GCTCCAGGAG TATGAACACC GGCTACTGGC AGCAGCAGAA 
GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TACACACCCC TGTTTGTGCT CACAGCCATG 
AACATCCGCG GCATGTGCTT GCTGTCCTAC AGCAGTTCCA GTGCCTGCCC TGTCAACCAG 
ACCAGCTCCT ACCTGTGTGC GGCTAAAGAG GCCTTCGAGA TCGGCCTGCT GACCAAGACT 
GACAACACGC CGGCCGCCGG GAAGCAGGAG CTCCACAGCC TCATCAAGGC AGCCTTCGGC 
CTGGCCACTG TGCACCGCCG ACTCCTCGGG AACACGGCCG CGGTGCGTGT GGCCCGCTGG 
CTGTGTGTGG AGGCCCTGGG GACACTGGCC AGGCTGGGCT CCTGCTCTGC AAGCCAGGAC 
CCAGAGGCAG TGTCAGGAGA GATCCTGTCT GCAATCAGCC AAGTGAGGCG GTGCCTCGGT 
GTCCAGAGCT TTGTAAACCC AGACGCCAGA GCTTGCATTC CGGAGAGTTT CCAGGGTGGC 
TTGGATGAGC CTCTTCTGCG GGGGAGCGTG GGTTTCCAGG AGATCCTCAC CGGCTACTCG 
CGGCACCACA GCTCAGTATG TGAGGTTTTT GAAAGCACAT GTGGACACAG CAAGATCCAC 
GAGAAACACG CAAAAACAGA CCTCTGCATC ACGGCCTTAG AAACAGAAAC CAAATCTCCA 
GACACACACA CCCTTGTGGA CCGGCCACGC TCTCCTGCAG GAAGGGGGAC ACTGTGCTAC 
TGGACAGACC AAATCGAGAG GGCAGGCATT GAGGCCTGGG CCCCACATCG GGACACGGAA 
CCTGGGGCAG AGCCACCCGG GCACCACAGC AGCAAGCTCC AGGAGAGGTC CCCGGGGGGC 
AGCTGGGGTT CTGGGAGCTG GAGCAGGCTG TCGGGGACCA GTTGTTCTGT GAGCTGGGAG 
GAGGTGAGCG CTGCGGGGGT GCCCAGCTCA GCTGGGGGCA CAGAGCACCT CGTGGACAGC 
CGGTGCCACA CTGCCTCATG TGAGGAGCTT GAGCCAGACA TCGAGGGCAG AGCCGGGCAC 
TTGGTGCCTT GGGATCTGCG GGGTCTGTCT CTCCGGGTGC CCAAGGATGA CTTTACAGAG 
ACCTCCCCAA GTCCTCTACA CGGAGCCCCG TCCTCACCAA ACCTCGCACC CCACAGCTCT 
GGCGACACCC AGGCAGTCCC TGTGGCCGAG CTGGCGGGGG CTCCAGGGGA CACCTGCGAA 
GTCAGAGGGA CTCTCTCGGG ACTCCCCCGC GCTTCTCCGG CTCCTGGTTT CGTCGGGCAC 
AGGGACACGG CCGCGTGGCA TTCCGTGCAG AACAACAACT TTGAAATTCT TGATTTCGCA 
GACACCAGCT GTTATGTCCC AGGCGGGCGT GGGGTAGCCA GGAAAACAGA AGCCCATGAT 
CCAGACCCAG GCCGCACGTG GGTGGACCCC GATGCAGAGA CTGCAGAAGG CACCCAGGCA 
GAGCCAGGTG GTTCGCGGGG CGCCAGTGCC AGGCCGGGGA ACAGCTCTCT CCCTCCCTGT 
CGCCCCACCT GCCCTGAGAC CAGGGGCTCC GCGGAGGAGC CAGGAGTCGA CCCCGAGGGC 
CCCACGGAGG TCGAGGGCGC CCCCTCTGCG GCTGGACCCC CCGGGCTCCC TTCGGGTGGC 
GGTAGCACCT TCCCTGTCCC CAGCGAGAGC TGTTCCTCCA CAGAGGAGCC GGGCCTGGGC 
GCCTTCTCCA GCGGCTCCCC GGGCTGCTCC AGCCCCTGGT CCCTGCTGGA CTCCTGCGGC 
GGCTCCGCGG CAGCCCGGCC AGGGCCGGAG CAGGAGGCGC TAGAGGCGCG CACGCTGCGG 
CCCGCGGACC TGCGGGAGCT GCTGGCCGGC GTGGGCCATG GCTGGCTGCG GCGGAGGCTG 
GAGGGCACCG GCGTGTTCAA GCCCGGCCGG CCGCGCGGGG CGCACGATGG TCTTCTGTTA 
AAATATTCCA AAAAGTCTGA ACTGTGGACA GCCCAGGAAA CGGCGGTGTA TCTGGGGGAC 
TACCTGGATG TGAAGAAGAA AGGCAAACAG AGGAACGCCT TCTGGGTTCA CCATCTTCAC 
CAAGAAGAAA CCCTGGGGAG GTACGTTGGG AAGGAATACA AAGAACAGAA GGGGCTCTGG 
CATCACTTCG CTGATGTGGA GAGGCAGATG ACCGCACAGC ACTACGTGAC AGAGTTCAAC 
AAGAAACTCT ATGAGCAAAA TATCCCAACA CAGATATTCT ACATCCCGTC CACAGTGCTG 
CTGATTTTAG AAGGCAACAC AATAACGGGA TGTATCAGTG TGGAGCCTTA CATACCAGGA 
GAGTTTGTAA AATTATCAAA CAACACAAAA GTGGTGAAAA CAGAGTACAA AGCCACAGAA 
TACGGCTTGG CTTATGGCCA TTTTTCTTAT GAGTTTTCTA ATCATAGAGA TGTTGTGGTT 
GACTTACAAG GCTGGGTGAC TGGTAATGGC AAAGGGTTAA TTTACCTCAC AGATCCCCAG 
ATCCACTCTG TTGATCAGAA AGACGTCACT ACCAATTTTG GAAAGAGAGG AATCTTTTAC 
TTCTTTAATA ACCAGCATGT GACATGTAAT GACATCTGCC ATCGTCTTTG TCTGACCCGA 
CCTTCAGAAG GAAACCTGAA CGAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpk1 ( XM_013150619.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013006073.1 Cavia porcellus alpha-kinase 1 (Alpk1), transcript variant X2, mRNA.

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CloneID	ODo28351
Clone ID Related Accession (Same CDS sequence)	XM_013150619.1
Accession Version	XM_013150619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	alpha-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176376.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCCGA GGAGGACAAG AGGGAGGACC AGCGATGCCG AGGTGGCCCT GCGTGCCTCC 
ATCCGGGCCC AGGACTGCAC AGTGGCCACA GCCGTTATCT TCCTGCTGGA CCGTGTGCTC 
TACAGGCTGG ACGGCACAGG CAGCCTCCTG CGGGTGGCAC GGCGCCTGCA CCGCCTGCAG 
CCCAGCACAC CCATCGCACC ACAGCTGCTT ATCCGACAGG CGCGTGTCTC GGTTAACGCA 
GGGAAGCTCC TGAAAGCAGA GTACATCCTC AGCAGCCTCA TCAGCAACCA CGGAGCCACA 
GGTACCTGGC TCTACAGAAA TGAAAGCGAC AAGGTCCTGG TGCAGTCAGT CTGTGTACAG 
ATCCGAGGGC AAATTCTGCA AAAGCTTGGC ATGTGGTATG AAGCCGCCGA GTTAACAAGT 
GCCTCCATCG TTGGATATTT GACGCTCCCT CTGCCGGATA AAAAGGGCCT CTCTGCGTCA 
CTTGGGATTC TGGCAGACAT CTTTGTTTCC ATGAGTGAGA ACGACTATGA GAGATTTAAG 
AACAACCCCC AGGTTAACTT GGCCCTGCTC CAGGAGTATG AACACCGGCT ACTGGCAGCA 
GCAGAAGCCT GCAAGCTGGC AGCTGCCTTC AGTGCCTACA CACCCCTGTT TGTGCTCACA 
GCCATGAACA TCCGCGGCAT GTGCTTGCTG TCCTACAGCA GTTCCAGTGC CTGCCCTGTC 
AACCAGACCA GCTCCTACCT GTGTGCGGCT AAAGAGGCCT TCGAGATCGG CCTGCTGACC 
AAGACTGACA ACACGCCGGC CGCCGGGAAG CAGGAGCTCC ACAGCCTCAT CAAGGCAGCC 
TTCGGCCTGG CCACTGTGCA CCGCCGACTC CTCGGGAACA CGGCCGCGGT GCGTGTGGCC 
CGCTGGCTGT GTGTGGAGGC CCTGGGGACA CTGGCCAGGC TGGGCTCCTG CTCTGCAAGC 
CAGGACCCAG AGGCAGTGTC AGGAGAGATC CTGTCTGCAA TCAGCCAAGT GAGGCGGTGC 
CTCGGTGTCC AGAGCTTTGT AAACCCAGAC GCCAGAGCTT GCATTCCGGA GAGTTTCCAG 
GGTGGCTTGG ATGAGCCTCT TCTGCGGGGG AGCGTGGGTT TCCAGGAGAT CCTCACCGGC 
TACTCGCGGC ACCACAGCTC AGTATGTGAG GTTTTTGAAA GCACATGTGG ACACAGCAAG 
ATCCACGAGA AACACGCAAA AACAGACCTC TGCATCACGG CCTTAGAAAC AGAAACCAAA 
TCTCCAGACA CACACACCCT TGTGGACCGG CCACGCTCTC CTGCAGGAAG GGGGACACTG 
TGCTACTGGA CAGACCAAAT CGAGAGGGCA GGCATTGAGG CCTGGGCCCC ACATCGGGAC 
ACGGAACCTG GGGCAGAGCC ACCCGGGCAC CACAGCAGCA AGCTCCAGGA GAGGTCCCCG 
GGGGGCAGCT GGGGTTCTGG GAGCTGGAGC AGGCTGTCGG GGACCAGTTG TTCTGTGAGC 
TGGGAGGAGG TGAGCGCTGC GGGGGTGCCC AGCTCAGCTG GGGGCACAGA GCACCTCGTG 
GACAGCCGGT GCCACACTGC CTCATGTGAG GAGCTTGAGC CAGACATCGA GGGCAGAGCC 
GGGCACTTGG TGCCTTGGGA TCTGCGGGGT CTGTCTCTCC GGGTGCCCAA GGATGACTTT 
ACAGAGACCT CCCCAAGTCC TCTACACGGA GCCCCGTCCT CACCAAACCT CGCACCCCAC 
AGCTCTGGCG ACACCCAGGC AGTCCCTGTG GCCGAGCTGG CGGGGGCTCC AGGGGACACC 
TGCGAAGTCA GAGGGACTCT CTCGGGACTC CCCCGCGCTT CTCCGGCTCC TGGTTTCGTC 
GGGCACAGGG ACACGGCCGC GTGGCATTCC GTGCAGAACA ACAACTTTGA AATTCTTGAT 
TTCGCAGACA CCAGCTGTTA TGTCCCAGGC GGGCGTGGGG TAGCCAGGAA AACAGAAGCC 
CATGATCCAG ACCCAGGCCG CACGTGGGTG GACCCCGATG CAGAGACTGC AGAAGGCACC 
CAGGCAGAGC CAGGTGGTTC GCGGGGCGCC AGTGCCAGGC CGGGGAACAG CTCTCTCCCT 
CCCTGTCGCC CCACCTGCCC TGAGACCAGG GGCTCCGCGG AGGAGCCAGG AGTCGACCCC 
GAGGGCCCCA CGGAGGTCGA GGGCGCCCCC TCTGCGGCTG GACCCCCCGG GCTCCCTTCG 
GGTGGCGGTA GCACCTTCCC TGTCCCCAGC GAGAGCTGTT CCTCCACAGA GGAGCCGGGC 
CTGGGCGCCT TCTCCAGCGG CTCCCCGGGC TGCTCCAGCC CCTGGTCCCT GCTGGACTCC 
TGCGGCGGCT CCGCGGCAGC CCGGCCAGGG CCGGAGCAGG AGGCGCTAGA GGCGCGCACG 
CTGCGGCCCG CGGACCTGCG GGAGCTGCTG GCCGGCGTGG GCCATGGCTG GCTGCGGCGG 
AGGCTGGAGG GCACCGGCGT GTTCAAGCCC GGCCGGCCGC GCGGGGCGCA CGATGGTCTT 
CTGTTAAAAT ATTCCAAAAA GTCTGAACTG TGGACAGCCC AGGAAACGGC GGTGTATCTG 
GGGGACTACC TGGATGTGAA GAAGAAAGGC AAACAGAGGA ACGCCTTCTG GGTTCACCAT 
CTTCACCAAG AAGAAACCCT GGGGAGGTAC GTTGGGAAGG AATACAAAGA ACAGAAGGGG 
CTCTGGCATC ACTTCGCTGA TGTGGAGAGG CAGATGACCG CACAGCACTA CGTGACAGAG 
TTCAACAAGA AACTCTATGA GCAAAATATC CCAACACAGA TATTCTACAT CCCGTCCACA 
GTGCTGCTGA TTTTAGAAGG CAACACAATA ACGGGATGTA TCAGTGTGGA GCCTTACATA 
CCAGGAGAGT TTGTAAAATT ATCAAACAAC ACAAAAGTGG TGAAAACAGA GTACAAAGCC 
ACAGAATACG GCTTGGCTTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT 
GTGGTTGACT TACAAGGCTG GGTGACTGGT AATGGCAAAG GGTTAATTTA CCTCACAGAT 
CCCCAGATCC ACTCTGTTGA TCAGAAAGAC GTCACTACCA ATTTTGGAAA GAGAGGAATC 
TTTTACTTCT TTAATAACCA GCATGTGACA TGTAATGACA TCTGCCATCG TCTTTGTCTG 
ACCCGACCTT CAGAAGGAAA CCTGAACGAG CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013006073.1
CDS	80..3295
Translation	MCPRRTRGRTSDAEVALRASIRAQDCTVATAVIFLLDRVLYRLDGTGSLLRVARRLHRLQPSTPIAPQLLIRQARVSVNAGKLLKAEYILSSLISNHGATGTWLYRNESDKVLVQSVCVQIRGQILQKLGMWYEAAELTSASIVGYLTLPLPDKKGLSASLGILADIFVSMSENDYERFKNNPQVNLALLQEYEHRLLAAAEACKLAAAFSAYTPLFVLTAMNIRGMCLLSYSSSSACPVNQTSSYLCAAKEAFEIGLLTKTDNTPAAGKQELHSLIKAAFGLATVHRRLLGNTAAVRVARWLCVEALGTLARLGSCSASQDPEAVSGEILSAISQVRRCLGVQSFVNPDARACIPESFQGGLDEPLLRGSVGFQEILTGYSRHHSSVCEVFESTCGHSKIHEKHAKTDLCITALETETKSPDTHTLVDRPRSPAGRGTLCYWTDQIERAGIEAWAPHRDTEPGAEPPGHHSSKLQERSPGGSWGSGSWSRLSGTSCSVSWEEVSAAGVPSSAGGTEHLVDSRCHTASCEELEPDIEGRAGHLVPWDLRGLSLRVPKDDFTETSPSPLHGAPSSPNLAPHSSGDTQAVPVAELAGAPGDTCEVRGTLSGLPRASPAPGFVGHRDTAAWHSVQNNNFEILDFADTSCYVPGGRGVARKTEAHDPDPGRTWVDPDAETAEGTQAEPGGSRGASARPGNSSLPPCRPTCPETRGSAEEPGVDPEGPTEVEGAPSAAGPPGLPSGGGSTFPVPSESCSSTEEPGLGAFSSGSPGCSSPWSLLDSCGGSAAARPGPEQEALEARTLRPADLRELLAGVGHGWLRRRLEGTGVFKPGRPRGAHDGLLLKYSKKSELWTAQETAVYLGDYLDVKKKGKQRNAFWVHHLHQEETLGRYVGKEYKEQKGLWHHFADVERQMTAQHYVTEFNKKLYEQNIPTQIFYIPSTVLLILEGNTITGCISVEPYIPGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRDVVVDLQGWVTGNGKGLIYLTDPQIHSVDQKDVTTNFGKRGIFYFFNNQHVTCNDICHRLCLTRPSEGNLNEP

Target ORF information:

RefSeq Version	XM_013150619.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus alpha-kinase 1 (Alpk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013150619.1

ORF Insert Sequence:

ATGTGTCCGA GGAGGACAAG AGGGAGGACC AGCGATGCCG AGGTGGCCCT GCGTGCCTCC 
ATCCGGGCCC AGGACTGCAC AGTGGCCACA GCCGTTATCT TCCTGCTGGA CCGTGTGCTC 
TACAGGCTGG ACGGCACAGG CAGCCTCCTG CGGGTGGCAC GGCGCCTGCA CCGCCTGCAG 
CCCAGCACAC CCATCGCACC ACAGCTGCTT ATCCGACAGG CGCGTGTCTC GGTTAACGCA 
GGGAAGCTCC TGAAAGCAGA GTACATCCTC AGCAGCCTCA TCAGCAACCA CGGAGCCACA 
GGTACCTGGC TCTACAGAAA TGAAAGCGAC AAGGTCCTGG TGCAGTCAGT CTGTGTACAG 
ATCCGAGGGC AAATTCTGCA AAAGCTTGGC ATGTGGTATG AAGCCGCCGA GTTAACAAGT 
GCCTCCATCG TTGGATATTT GACGCTCCCT CTGCCGGATA AAAAGGGCCT CTCTGCGTCA 
CTTGGGATTC TGGCAGACAT CTTTGTTTCC ATGAGTGAGA ACGACTATGA GAGATTTAAG 
AACAACCCCC AGGTTAACTT GGCCCTGCTC CAGGAGTATG AACACCGGCT ACTGGCAGCA 
GCAGAAGCCT GCAAGCTGGC AGCTGCCTTC AGTGCCTACA CACCCCTGTT TGTGCTCACA 
GCCATGAACA TCCGCGGCAT GTGCTTGCTG TCCTACAGCA GTTCCAGTGC CTGCCCTGTC 
AACCAGACCA GCTCCTACCT GTGTGCGGCT AAAGAGGCCT TCGAGATCGG CCTGCTGACC 
AAGACTGACA ACACGCCGGC CGCCGGGAAG CAGGAGCTCC ACAGCCTCAT CAAGGCAGCC 
TTCGGCCTGG CCACTGTGCA CCGCCGACTC CTCGGGAACA CGGCCGCGGT GCGTGTGGCC 
CGCTGGCTGT GTGTGGAGGC CCTGGGGACA CTGGCCAGGC TGGGCTCCTG CTCTGCAAGC 
CAGGACCCAG AGGCAGTGTC AGGAGAGATC CTGTCTGCAA TCAGCCAAGT GAGGCGGTGC 
CTCGGTGTCC AGAGCTTTGT AAACCCAGAC GCCAGAGCTT GCATTCCGGA GAGTTTCCAG 
GGTGGCTTGG ATGAGCCTCT TCTGCGGGGG AGCGTGGGTT TCCAGGAGAT CCTCACCGGC 
TACTCGCGGC ACCACAGCTC AGTATGTGAG GTTTTTGAAA GCACATGTGG ACACAGCAAG 
ATCCACGAGA AACACGCAAA AACAGACCTC TGCATCACGG CCTTAGAAAC AGAAACCAAA 
TCTCCAGACA CACACACCCT TGTGGACCGG CCACGCTCTC CTGCAGGAAG GGGGACACTG 
TGCTACTGGA CAGACCAAAT CGAGAGGGCA GGCATTGAGG CCTGGGCCCC ACATCGGGAC 
ACGGAACCTG GGGCAGAGCC ACCCGGGCAC CACAGCAGCA AGCTCCAGGA GAGGTCCCCG 
GGGGGCAGCT GGGGTTCTGG GAGCTGGAGC AGGCTGTCGG GGACCAGTTG TTCTGTGAGC 
TGGGAGGAGG TGAGCGCTGC GGGGGTGCCC AGCTCAGCTG GGGGCACAGA GCACCTCGTG 
GACAGCCGGT GCCACACTGC CTCATGTGAG GAGCTTGAGC CAGACATCGA GGGCAGAGCC 
GGGCACTTGG TGCCTTGGGA TCTGCGGGGT CTGTCTCTCC GGGTGCCCAA GGATGACTTT 
ACAGAGACCT CCCCAAGTCC TCTACACGGA GCCCCGTCCT CACCAAACCT CGCACCCCAC 
AGCTCTGGCG ACACCCAGGC AGTCCCTGTG GCCGAGCTGG CGGGGGCTCC AGGGGACACC 
TGCGAAGTCA GAGGGACTCT CTCGGGACTC CCCCGCGCTT CTCCGGCTCC TGGTTTCGTC 
GGGCACAGGG ACACGGCCGC GTGGCATTCC GTGCAGAACA ACAACTTTGA AATTCTTGAT 
TTCGCAGACA CCAGCTGTTA TGTCCCAGGC GGGCGTGGGG TAGCCAGGAA AACAGAAGCC 
CATGATCCAG ACCCAGGCCG CACGTGGGTG GACCCCGATG CAGAGACTGC AGAAGGCACC 
CAGGCAGAGC CAGGTGGTTC GCGGGGCGCC AGTGCCAGGC CGGGGAACAG CTCTCTCCCT 
CCCTGTCGCC CCACCTGCCC TGAGACCAGG GGCTCCGCGG AGGAGCCAGG AGTCGACCCC 
GAGGGCCCCA CGGAGGTCGA GGGCGCCCCC TCTGCGGCTG GACCCCCCGG GCTCCCTTCG 
GGTGGCGGTA GCACCTTCCC TGTCCCCAGC GAGAGCTGTT CCTCCACAGA GGAGCCGGGC 
CTGGGCGCCT TCTCCAGCGG CTCCCCGGGC TGCTCCAGCC CCTGGTCCCT GCTGGACTCC 
TGCGGCGGCT CCGCGGCAGC CCGGCCAGGG CCGGAGCAGG AGGCGCTAGA GGCGCGCACG 
CTGCGGCCCG CGGACCTGCG GGAGCTGCTG GCCGGCGTGG GCCATGGCTG GCTGCGGCGG 
AGGCTGGAGG GCACCGGCGT GTTCAAGCCC GGCCGGCCGC GCGGGGCGCA CGATGGTCTT 
CTGTTAAAAT ATTCCAAAAA GTCTGAACTG TGGACAGCCC AGGAAACGGC GGTGTATCTG 
GGGGACTACC TGGATGTGAA GAAGAAAGGC AAACAGAGGA ACGCCTTCTG GGTTCACCAT 
CTTCACCAAG AAGAAACCCT GGGGAGGTAC GTTGGGAAGG AATACAAAGA ACAGAAGGGG 
CTCTGGCATC ACTTCGCTGA TGTGGAGAGG CAGATGACCG CACAGCACTA CGTGACAGAG 
TTCAACAAGA AACTCTATGA GCAAAATATC CCAACACAGA TATTCTACAT CCCGTCCACA 
GTGCTGCTGA TTTTAGAAGG CAACACAATA ACGGGATGTA TCAGTGTGGA GCCTTACATA 
CCAGGAGAGT TTGTAAAATT ATCAAACAAC ACAAAAGTGG TGAAAACAGA GTACAAAGCC 
ACAGAATACG GCTTGGCTTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT 
GTGGTTGACT TACAAGGCTG GGTGACTGGT AATGGCAAAG GGTTAATTTA CCTCACAGAT 
CCCCAGATCC ACTCTGTTGA TCAGAAAGAC GTCACTACCA ATTTTGGAAA GAGAGGAATC 
TTTTACTTCT TTAATAACCA GCATGTGACA TGTAATGACA TCTGCCATCG TCTTTGTCTG 
ACCCGACCTT CAGAAGGAAA CCTGAACGAG CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Alpk1 cDNA ORF clone, Cavia porcellus(domestic guinea pig)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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