Agrn gene cDNA ORF clone, Cavia porcellus(domestic guinea pig)

mRNA	Protein	Name
XM_023565389.1	XP_023421157.1	agrin isoform X1
XM_023565393.1	XP_023421161.1	agrin isoform X5
XM_013153374.2	XP_013008828.1	agrin isoform X7
XM_013153376.2	XP_013008830.1	agrin isoform X8
XM_013153379.1	XP_013008833.1	agrin isoform X9
XM_013153362.1	XP_013008816.1	agrin isoform X2
XM_004999693.2	XP_004999750.2	agrin isoform X8
XM_013153365.1	XP_013008819.1	agrin isoform X3
XM_013153371.2	XP_013008825.1	agrin isoform X6
XM_013153374.1	XP_013008828.1	agrin isoform X5
XM_013153376.1	XP_013008830.1	agrin isoform X6
XM_023565392.1	XP_023421160.1	agrin isoform X4
XM_013153359.2	XP_013008813.1	agrin isoform X2
XM_013153359.1	XP_013008813.1	agrin isoform X1
XM_013153362.2	XP_013008816.1	agrin isoform X3
XM_013153371.1	XP_013008825.1	agrin isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODo01579	XM_013153359.2 Latest version!	Cavia porcellus agrin (Agrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo01580	XM_013153362.2 Latest version!	Cavia porcellus agrin (Agrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo01582	XM_013153365.1 Latest version!	Cavia porcellus agrin (Agrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo01584	XM_013153371.2 Latest version!	Cavia porcellus agrin (Agrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo01585	XM_013153374.2 Latest version!	Cavia porcellus agrin (Agrn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo01586	XM_013153376.2 Latest version!	Cavia porcellus agrin (Agrn), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo28105	XM_013153379.1 Latest version!	Cavia porcellus agrin (Agrn), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODo28106	XM_004999693.2 Latest version!	Cavia porcellus agrin (Agrn), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo41202	XM_023565389.1 Latest version!	Cavia porcellus agrin (Agrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo41203	XM_023565392.1 Latest version!	Cavia porcellus agrin (Agrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo41204	XM_023565393.1 Latest version!	Cavia porcellus agrin (Agrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODo01585	XM_013153374.1	Cavia porcellus agrin (Agrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo01586	XM_013153376.1	Cavia porcellus agrin (Agrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo01579	XM_013153359.1	Cavia porcellus agrin (Agrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo01580	XM_013153362.1	Cavia porcellus agrin (Agrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODo01584	XM_013153371.1	Cavia porcellus agrin (Agrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

Agrn ( XM_013153359.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008813.1 Cavia porcellus agrin (Agrn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODo01579
Clone ID Related Accession (Same CDS sequence)	XM_013153359.2 , XM_013153359.1
Accession Version	XM_013153359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_013153359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
AAACTGGCCA ATGAGATCCC ATTCCCGGAA GCTCCCGATT CCGGGTCCTT TCACAGCGAG 
AAGGCGCTGC AGAGCAACCA CTTCGAGCTG AGCCTGCGCA CCGAGGCCAC TCAGGGGCTG 
GTGCTGTGGA GCGGCAAGGC CACAGAGCGT GCAGACTATG TGGCTCTGGC CATCGTGGAT 
GGGTACCTGC AGCTGAGCTA CGACCTGGGC TCCCAGCCCG TGGTGCTGCG CTCCACTGTG 
AAGGTCAACA CCAATCACTG GCTGCGGGTT AAGGCTCACA GGGAGCAGAG GGAAGGGTCC 
CTTCAGGTGG GCAATGAAGC CCCTGTGACT GGCTCGTCTC CACTGGGCTC CACACAGCTG 
GACACAGATG GAGCCCTGTG GCTCGGGGGC CTTCAGAAGC TGCCTGTGGG CCAGGCCCTG 
CCCAAGGCCT ACAGTACAGG CTTCGTGGGC TGCTTACGGG ATGTAGTGGT GAGCGGGCGC 
CCACTGCACC TGCTGGAAGA TGCAGTCACC AGGCCAGAGC TGCGGCCCTG CCCTGCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008813.1
CDS	187..6069
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESKLANEIPFPEAPDSGSFHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153359.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153359.2

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
AAACTGGCCA ATGAGATCCC ATTCCCGGAA GCTCCCGATT CCGGGTCCTT TCACAGCGAG 
AAGGCGCTGC AGAGCAACCA CTTCGAGCTG AGCCTGCGCA CCGAGGCCAC TCAGGGGCTG 
GTGCTGTGGA GCGGCAAGGC CACAGAGCGT GCAGACTATG TGGCTCTGGC CATCGTGGAT 
GGGTACCTGC AGCTGAGCTA CGACCTGGGC TCCCAGCCCG TGGTGCTGCG CTCCACTGTG 
AAGGTCAACA CCAATCACTG GCTGCGGGTT AAGGCTCACA GGGAGCAGAG GGAAGGGTCC 
CTTCAGGTGG GCAATGAAGC CCCTGTGACT GGCTCGTCTC CACTGGGCTC CACACAGCTG 
GACACAGATG GAGCCCTGTG GCTCGGGGGC CTTCAGAAGC TGCCTGTGGG CCAGGCCCTG 
CCCAAGGCCT ACAGTACAGG CTTCGTGGGC TGCTTACGGG ATGTAGTGGT GAGCGGGCGC 
CCACTGCACC TGCTGGAAGA TGCAGTCACC AGGCCAGAGC TGCGGCCCTG CCCTGCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_013153359.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008813.1 Cavia porcellus agrin (Agrn), transcript variant X1, mRNA.

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CloneID	ODo01579
Clone ID Related Accession (Same CDS sequence)	XM_013153359.2 , XM_013153359.1
Accession Version	XM_013153359.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
AAACTGGCCA ATGAGATCCC ATTCCCGGAA GCTCCCGATT CCGGGTCCTT TCACAGCGAG 
AAGGCGCTGC AGAGCAACCA CTTCGAGCTG AGCCTGCGCA CCGAGGCCAC TCAGGGGCTG 
GTGCTGTGGA GCGGCAAGGC CACAGAGCGT GCAGACTATG TGGCTCTGGC CATCGTGGAT 
GGGTACCTGC AGCTGAGCTA CGACCTGGGC TCCCAGCCCG TGGTGCTGCG CTCCACTGTG 
AAGGTCAACA CCAATCACTG GCTGCGGGTT AAGGCTCACA GGGAGCAGAG GGAAGGGTCC 
CTTCAGGTGG GCAATGAAGC CCCTGTGACT GGCTCGTCTC CACTGGGCTC CACACAGCTG 
GACACAGATG GAGCCCTGTG GCTCGGGGGC CTTCAGAAGC TGCCTGTGGG CCAGGCCCTG 
CCCAAGGCCT ACAGTACAGG CTTCGTGGGC TGCTTACGGG ATGTAGTGGT GAGCGGGCGC 
CCACTGCACC TGCTGGAAGA TGCAGTCACC AGGCCAGAGC TGCGGCCCTG CCCTGCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008813.1
CDS	208..6090
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESKLANEIPFPEAPDSGSFHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153359.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153359.1

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
AAACTGGCCA ATGAGATCCC ATTCCCGGAA GCTCCCGATT CCGGGTCCTT TCACAGCGAG 
AAGGCGCTGC AGAGCAACCA CTTCGAGCTG AGCCTGCGCA CCGAGGCCAC TCAGGGGCTG 
GTGCTGTGGA GCGGCAAGGC CACAGAGCGT GCAGACTATG TGGCTCTGGC CATCGTGGAT 
GGGTACCTGC AGCTGAGCTA CGACCTGGGC TCCCAGCCCG TGGTGCTGCG CTCCACTGTG 
AAGGTCAACA CCAATCACTG GCTGCGGGTT AAGGCTCACA GGGAGCAGAG GGAAGGGTCC 
CTTCAGGTGG GCAATGAAGC CCCTGTGACT GGCTCGTCTC CACTGGGCTC CACACAGCTG 
GACACAGATG GAGCCCTGTG GCTCGGGGGC CTTCAGAAGC TGCCTGTGGG CCAGGCCCTG 
CCCAAGGCCT ACAGTACAGG CTTCGTGGGC TGCTTACGGG ATGTAGTGGT GAGCGGGCGC 
CCACTGCACC TGCTGGAAGA TGCAGTCACC AGGCCAGAGC TGCGGCCCTG CCCTGCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_013153362.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008816.1 Cavia porcellus agrin (Agrn), transcript variant X2, mRNA.

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CloneID	ODo01580
Clone ID Related Accession (Same CDS sequence)	XM_013153362.1 , XM_013153362.2
Accession Version	XM_013153362.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGCCTT CTTCACAGGA GCTGCTACAG GAGCCACGGC TGCAGTGGCC 
ACAGCCAGAG CCACCACCAC AGGCCAGCTG CCCTCTGCTG TAACCCCTCG GGCCTACCCC 
AGTCACACCA GCCGACCTAC TGGCAAGACC ACAGCACCCC TCACCACACG CCGGCCTCTC 
ACTACGCTCC CCAGCCGCAT GGCAGGACAC CAACCCATTC CCCCAGACCC TCAGCAGCCA 
CTGAGGCCCT GTGCCTCCCA GCCCTGCCTC TATGGGGGCA CGTGCTGGGA CCAGGACTCT 
GGCCAAGGCT TCACTTGCAG CTGCCCAGCG GGCAGGGGCG GCATCATCTG CGAGGAGGTA 
TTGCGCCCCT CTCTGCCTGC TTTTGGAGGC CACTCCTTCC TGGCCTTCCC CACCCTACGC 
GCCTACCACA CACTGCGCCT GGCACTAGAA TTCCGAACAC TGGAGCTGCA GGGGCTGCTG 
CTCTACAATG GGAACAACCG TGGCAAAGAC TTCTTGGCGC TGGCTCTACA GGGTGGGCGG 
GTGCAGCTCA GGTTTGACAC AGGTTCTGGG CCAGCAGTGT TGACCAGTTC CGTGCCTGTG 
AAACTGGGCC GGTGGCACCG CCTAGAACTG TCAAGGCATT GGCGCCAGGG CACACTGTCT 
GTGGATGGCG AGAGCCCTGT TGTGGGCGAG AGCCCCAGTG GCACGGATGG CCTTAACCTG 
GACACGGACC TTTTTGTGGG TGGCGTCCCA GAGGACCAGG CTGCTGTGGT GCTTGAGCGG 
ACCTTCGTTG CAGTTGGCCT GAAAGGCTGC ATCCGGTTGC TGGACATCAA CAACCAGCGG 
CTGGAGCTGG GCAGCTGGCA AGGAGCTTCG ACCCAGAGCT CTGGCGTGGG CGAGTGTGGG 
GAAAACCCCT GTGTGCCCAA CCCCTGCAGC AGTGGGGCGC CATGCCAGGC ACTTGACGAT 
GGCATGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGTC TGACCTGTGC CAGTGAGAAG 
AACCCCTGCC AGCCAAACCC TTGCCATGGA GCAGCCCCTT GCCGTGTACT ACCCGAGGGA 
GGGCCGAAGT GCGAGTGCCC CCTTGGGCGT GGGGGCGCCC TCTGCCAGAC AGACTTGGAG 
AAGGACCATG GCTCCCAGCC CTTCATGGCG GATTTCAGCG GCTTCTCCTA TCTGGAGCTG 
AAAGGCCTGT ACACCTTTGA AAGAGACCTG GGAGAAAAGA TGGCGCTGGA GGTTGTGTTC 
CTGGCCCGTG GCCCCAGTGG CTTGCTCCTT TACAATGGGC AGAAGACAGA TGGCAGAGGA 
GACTTTGTGT CGCTGGCCCT GCGTGACTGG CATCTGGAAT TCCGTTATGA CCTGGGCAAG 
GGCCCTGCAG TCATCAGGAG CAAGGAGCCA GTCGCCGTGG ACACCTGGAC AAGGGTCTCA 
CTGGAGCGCA ATGGCCGCAA GGGTGCCATG CGTGTGGGCG ATGGGCCCCG AGTGCTGGGG 
GAGTCCCCGA AATCCCGCAA GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
AAACTGGCCA ATGAGATCCC ATTCCCGGAA GCTCCCGATT CCGGGTCCTT TCACAGCGAG 
AAGGCGCTGC AGAGCAACCA CTTCGAGCTG AGCCTGCGCA CCGAGGCCAC TCAGGGGCTG 
GTGCTGTGGA GCGGCAAGGC CACAGAGCGT GCAGACTATG TGGCTCTGGC CATCGTGGAT 
GGGTACCTGC AGCTGAGCTA CGACCTGGGC TCCCAGCCCG TGGTGCTGCG CTCCACTGTG 
AAGGTCAACA CCAATCACTG GCTGCGGGTT AAGGCTCACA GGGAGCAGAG GGAAGGGTCC 
CTTCAGGTGG GCAATGAAGC CCCTGTGACT GGCTCGTCTC CACTGGGCTC CACACAGCTG 
GACACAGATG GAGCCCTGTG GCTCGGGGGC CTTCAGAAGC TGCCTGTGGG CCAGGCCCTG 
CCCAAGGCCT ACAGTACAGG CTTCGTGGGC TGCTTACGGG ATGTAGTGGT GAGCGGGCGC 
CCACTGCACC TGCTGGAAGA TGCAGTCACC AGGCCAGAGC TGCGGCCCTG CCCTGCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008816.1
CDS	211..6093
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESKLANEIPFPEAPDSGSFHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153362.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153362.1

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGCCTT CTTCACAGGA GCTGCTACAG GAGCCACGGC TGCAGTGGCC 
ACAGCCAGAG CCACCACCAC AGGCCAGCTG CCCTCTGCTG TAACCCCTCG GGCCTACCCC 
AGTCACACCA GCCGACCTAC TGGCAAGACC ACAGCACCCC TCACCACACG CCGGCCTCTC 
ACTACGCTCC CCAGCCGCAT GGCAGGACAC CAACCCATTC CCCCAGACCC TCAGCAGCCA 
CTGAGGCCCT GTGCCTCCCA GCCCTGCCTC TATGGGGGCA CGTGCTGGGA CCAGGACTCT 
GGCCAAGGCT TCACTTGCAG CTGCCCAGCG GGCAGGGGCG GCATCATCTG CGAGGAGGTA 
TTGCGCCCCT CTCTGCCTGC TTTTGGAGGC CACTCCTTCC TGGCCTTCCC CACCCTACGC 
GCCTACCACA CACTGCGCCT GGCACTAGAA TTCCGAACAC TGGAGCTGCA GGGGCTGCTG 
CTCTACAATG GGAACAACCG TGGCAAAGAC TTCTTGGCGC TGGCTCTACA GGGTGGGCGG 
GTGCAGCTCA GGTTTGACAC AGGTTCTGGG CCAGCAGTGT TGACCAGTTC CGTGCCTGTG 
AAACTGGGCC GGTGGCACCG CCTAGAACTG TCAAGGCATT GGCGCCAGGG CACACTGTCT 
GTGGATGGCG AGAGCCCTGT TGTGGGCGAG AGCCCCAGTG GCACGGATGG CCTTAACCTG 
GACACGGACC TTTTTGTGGG TGGCGTCCCA GAGGACCAGG CTGCTGTGGT GCTTGAGCGG 
ACCTTCGTTG CAGTTGGCCT GAAAGGCTGC ATCCGGTTGC TGGACATCAA CAACCAGCGG 
CTGGAGCTGG GCAGCTGGCA AGGAGCTTCG ACCCAGAGCT CTGGCGTGGG CGAGTGTGGG 
GAAAACCCCT GTGTGCCCAA CCCCTGCAGC AGTGGGGCGC CATGCCAGGC ACTTGACGAT 
GGCATGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGTC TGACCTGTGC CAGTGAGAAG 
AACCCCTGCC AGCCAAACCC TTGCCATGGA GCAGCCCCTT GCCGTGTACT ACCCGAGGGA 
GGGCCGAAGT GCGAGTGCCC CCTTGGGCGT GGGGGCGCCC TCTGCCAGAC AGACTTGGAG 
AAGGACCATG GCTCCCAGCC CTTCATGGCG GATTTCAGCG GCTTCTCCTA TCTGGAGCTG 
AAAGGCCTGT ACACCTTTGA AAGAGACCTG GGAGAAAAGA TGGCGCTGGA GGTTGTGTTC 
CTGGCCCGTG GCCCCAGTGG CTTGCTCCTT TACAATGGGC AGAAGACAGA TGGCAGAGGA 
GACTTTGTGT CGCTGGCCCT GCGTGACTGG CATCTGGAAT TCCGTTATGA CCTGGGCAAG 
GGCCCTGCAG TCATCAGGAG CAAGGAGCCA GTCGCCGTGG ACACCTGGAC AAGGGTCTCA 
CTGGAGCGCA ATGGCCGCAA GGGTGCCATG CGTGTGGGCG ATGGGCCCCG AGTGCTGGGG 
GAGTCCCCGA AATCCCGCAA GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
AAACTGGCCA ATGAGATCCC ATTCCCGGAA GCTCCCGATT CCGGGTCCTT TCACAGCGAG 
AAGGCGCTGC AGAGCAACCA CTTCGAGCTG AGCCTGCGCA CCGAGGCCAC TCAGGGGCTG 
GTGCTGTGGA GCGGCAAGGC CACAGAGCGT GCAGACTATG TGGCTCTGGC CATCGTGGAT 
GGGTACCTGC AGCTGAGCTA CGACCTGGGC TCCCAGCCCG TGGTGCTGCG CTCCACTGTG 
AAGGTCAACA CCAATCACTG GCTGCGGGTT AAGGCTCACA GGGAGCAGAG GGAAGGGTCC 
CTTCAGGTGG GCAATGAAGC CCCTGTGACT GGCTCGTCTC CACTGGGCTC CACACAGCTG 
GACACAGATG GAGCCCTGTG GCTCGGGGGC CTTCAGAAGC TGCCTGTGGG CCAGGCCCTG 
CCCAAGGCCT ACAGTACAGG CTTCGTGGGC TGCTTACGGG ATGTAGTGGT GAGCGGGCGC 
CCACTGCACC TGCTGGAAGA TGCAGTCACC AGGCCAGAGC TGCGGCCCTG CCCTGCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_013153362.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008816.1 Cavia porcellus agrin (Agrn), transcript variant X3, mRNA.

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CloneID	ODo01580
Clone ID Related Accession (Same CDS sequence)	XM_013153362.1 , XM_013153362.2
Accession Version	XM_013153362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_013153362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGCCTT CTTCACAGGA GCTGCTACAG GAGCCACGGC TGCAGTGGCC 
ACAGCCAGAG CCACCACCAC AGGCCAGCTG CCCTCTGCTG TAACCCCTCG GGCCTACCCC 
AGTCACACCA GCCGACCTAC TGGCAAGACC ACAGCACCCC TCACCACACG CCGGCCTCTC 
ACTACGCTCC CCAGCCGCAT GGCAGGACAC CAACCCATTC CCCCAGACCC TCAGCAGCCA 
CTGAGGCCCT GTGCCTCCCA GCCCTGCCTC TATGGGGGCA CGTGCTGGGA CCAGGACTCT 
GGCCAAGGCT TCACTTGCAG CTGCCCAGCG GGCAGGGGCG GCATCATCTG CGAGGAGGTA 
TTGCGCCCCT CTCTGCCTGC TTTTGGAGGC CACTCCTTCC TGGCCTTCCC CACCCTACGC 
GCCTACCACA CACTGCGCCT GGCACTAGAA TTCCGAACAC TGGAGCTGCA GGGGCTGCTG 
CTCTACAATG GGAACAACCG TGGCAAAGAC TTCTTGGCGC TGGCTCTACA GGGTGGGCGG 
GTGCAGCTCA GGTTTGACAC AGGTTCTGGG CCAGCAGTGT TGACCAGTTC CGTGCCTGTG 
AAACTGGGCC GGTGGCACCG CCTAGAACTG TCAAGGCATT GGCGCCAGGG CACACTGTCT 
GTGGATGGCG AGAGCCCTGT TGTGGGCGAG AGCCCCAGTG GCACGGATGG CCTTAACCTG 
GACACGGACC TTTTTGTGGG TGGCGTCCCA GAGGACCAGG CTGCTGTGGT GCTTGAGCGG 
ACCTTCGTTG CAGTTGGCCT GAAAGGCTGC ATCCGGTTGC TGGACATCAA CAACCAGCGG 
CTGGAGCTGG GCAGCTGGCA AGGAGCTTCG ACCCAGAGCT CTGGCGTGGG CGAGTGTGGG 
GAAAACCCCT GTGTGCCCAA CCCCTGCAGC AGTGGGGCGC CATGCCAGGC ACTTGACGAT 
GGCATGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGTC TGACCTGTGC CAGTGAGAAG 
AACCCCTGCC AGCCAAACCC TTGCCATGGA GCAGCCCCTT GCCGTGTACT ACCCGAGGGA 
GGGCCGAAGT GCGAGTGCCC CCTTGGGCGT GGGGGCGCCC TCTGCCAGAC AGACTTGGAG 
AAGGACCATG GCTCCCAGCC CTTCATGGCG GATTTCAGCG GCTTCTCCTA TCTGGAGCTG 
AAAGGCCTGT ACACCTTTGA AAGAGACCTG GGAGAAAAGA TGGCGCTGGA GGTTGTGTTC 
CTGGCCCGTG GCCCCAGTGG CTTGCTCCTT TACAATGGGC AGAAGACAGA TGGCAGAGGA 
GACTTTGTGT CGCTGGCCCT GCGTGACTGG CATCTGGAAT TCCGTTATGA CCTGGGCAAG 
GGCCCTGCAG TCATCAGGAG CAAGGAGCCA GTCGCCGTGG ACACCTGGAC AAGGGTCTCA 
CTGGAGCGCA ATGGCCGCAA GGGTGCCATG CGTGTGGGCG ATGGGCCCCG AGTGCTGGGG 
GAGTCCCCGA AATCCCGCAA GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
AAACTGGCCA ATGAGATCCC ATTCCCGGAA GCTCCCGATT CCGGGTCCTT TCACAGCGAG 
AAGGCGCTGC AGAGCAACCA CTTCGAGCTG AGCCTGCGCA CCGAGGCCAC TCAGGGGCTG 
GTGCTGTGGA GCGGCAAGGC CACAGAGCGT GCAGACTATG TGGCTCTGGC CATCGTGGAT 
GGGTACCTGC AGCTGAGCTA CGACCTGGGC TCCCAGCCCG TGGTGCTGCG CTCCACTGTG 
AAGGTCAACA CCAATCACTG GCTGCGGGTT AAGGCTCACA GGGAGCAGAG GGAAGGGTCC 
CTTCAGGTGG GCAATGAAGC CCCTGTGACT GGCTCGTCTC CACTGGGCTC CACACAGCTG 
GACACAGATG GAGCCCTGTG GCTCGGGGGC CTTCAGAAGC TGCCTGTGGG CCAGGCCCTG 
CCCAAGGCCT ACAGTACAGG CTTCGTGGGC TGCTTACGGG ATGTAGTGGT GAGCGGGCGC 
CCACTGCACC TGCTGGAAGA TGCAGTCACC AGGCCAGAGC TGCGGCCCTG CCCTGCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008816.1
CDS	189..6071
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESKLANEIPFPEAPDSGSFHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153362.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153362.2

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGCCTT CTTCACAGGA GCTGCTACAG GAGCCACGGC TGCAGTGGCC 
ACAGCCAGAG CCACCACCAC AGGCCAGCTG CCCTCTGCTG TAACCCCTCG GGCCTACCCC 
AGTCACACCA GCCGACCTAC TGGCAAGACC ACAGCACCCC TCACCACACG CCGGCCTCTC 
ACTACGCTCC CCAGCCGCAT GGCAGGACAC CAACCCATTC CCCCAGACCC TCAGCAGCCA 
CTGAGGCCCT GTGCCTCCCA GCCCTGCCTC TATGGGGGCA CGTGCTGGGA CCAGGACTCT 
GGCCAAGGCT TCACTTGCAG CTGCCCAGCG GGCAGGGGCG GCATCATCTG CGAGGAGGTA 
TTGCGCCCCT CTCTGCCTGC TTTTGGAGGC CACTCCTTCC TGGCCTTCCC CACCCTACGC 
GCCTACCACA CACTGCGCCT GGCACTAGAA TTCCGAACAC TGGAGCTGCA GGGGCTGCTG 
CTCTACAATG GGAACAACCG TGGCAAAGAC TTCTTGGCGC TGGCTCTACA GGGTGGGCGG 
GTGCAGCTCA GGTTTGACAC AGGTTCTGGG CCAGCAGTGT TGACCAGTTC CGTGCCTGTG 
AAACTGGGCC GGTGGCACCG CCTAGAACTG TCAAGGCATT GGCGCCAGGG CACACTGTCT 
GTGGATGGCG AGAGCCCTGT TGTGGGCGAG AGCCCCAGTG GCACGGATGG CCTTAACCTG 
GACACGGACC TTTTTGTGGG TGGCGTCCCA GAGGACCAGG CTGCTGTGGT GCTTGAGCGG 
ACCTTCGTTG CAGTTGGCCT GAAAGGCTGC ATCCGGTTGC TGGACATCAA CAACCAGCGG 
CTGGAGCTGG GCAGCTGGCA AGGAGCTTCG ACCCAGAGCT CTGGCGTGGG CGAGTGTGGG 
GAAAACCCCT GTGTGCCCAA CCCCTGCAGC AGTGGGGCGC CATGCCAGGC ACTTGACGAT 
GGCATGTTCC ACTGTCAGTG CCCACCTGGC CGCTTTGGTC TGACCTGTGC CAGTGAGAAG 
AACCCCTGCC AGCCAAACCC TTGCCATGGA GCAGCCCCTT GCCGTGTACT ACCCGAGGGA 
GGGCCGAAGT GCGAGTGCCC CCTTGGGCGT GGGGGCGCCC TCTGCCAGAC AGACTTGGAG 
AAGGACCATG GCTCCCAGCC CTTCATGGCG GATTTCAGCG GCTTCTCCTA TCTGGAGCTG 
AAAGGCCTGT ACACCTTTGA AAGAGACCTG GGAGAAAAGA TGGCGCTGGA GGTTGTGTTC 
CTGGCCCGTG GCCCCAGTGG CTTGCTCCTT TACAATGGGC AGAAGACAGA TGGCAGAGGA 
GACTTTGTGT CGCTGGCCCT GCGTGACTGG CATCTGGAAT TCCGTTATGA CCTGGGCAAG 
GGCCCTGCAG TCATCAGGAG CAAGGAGCCA GTCGCCGTGG ACACCTGGAC AAGGGTCTCA 
CTGGAGCGCA ATGGCCGCAA GGGTGCCATG CGTGTGGGCG ATGGGCCCCG AGTGCTGGGG 
GAGTCCCCGA AATCCCGCAA GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
AAACTGGCCA ATGAGATCCC ATTCCCGGAA GCTCCCGATT CCGGGTCCTT TCACAGCGAG 
AAGGCGCTGC AGAGCAACCA CTTCGAGCTG AGCCTGCGCA CCGAGGCCAC TCAGGGGCTG 
GTGCTGTGGA GCGGCAAGGC CACAGAGCGT GCAGACTATG TGGCTCTGGC CATCGTGGAT 
GGGTACCTGC AGCTGAGCTA CGACCTGGGC TCCCAGCCCG TGGTGCTGCG CTCCACTGTG 
AAGGTCAACA CCAATCACTG GCTGCGGGTT AAGGCTCACA GGGAGCAGAG GGAAGGGTCC 
CTTCAGGTGG GCAATGAAGC CCCTGTGACT GGCTCGTCTC CACTGGGCTC CACACAGCTG 
GACACAGATG GAGCCCTGTG GCTCGGGGGC CTTCAGAAGC TGCCTGTGGG CCAGGCCCTG 
CCCAAGGCCT ACAGTACAGG CTTCGTGGGC TGCTTACGGG ATGTAGTGGT GAGCGGGCGC 
CCACTGCACC TGCTGGAAGA TGCAGTCACC AGGCCAGAGC TGCGGCCCTG CCCTGCACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_013153365.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008819.1 Cavia porcellus agrin (Agrn), transcript variant X3, mRNA.

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CloneID	ODo01582
Clone ID Related Accession (Same CDS sequence)	XM_013153365.1
Accession Version	XM_013153365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGGAGC TGCTACAGGA GCCACGGCTG CAGTGGCCAC AGCCAGAGCC 
ACCACCACAG GCCAGCTGCC CTCTGCTGTA ACCCCTCGGG CCTACCCCAG TCACACCAGC 
CGACCTACTG GCAAGACCAC AGCACCCCTC ACCACACGCC GGCCTCTCAC TACGCTCCCC 
AGCCGCATGG CAGGACACCA ACCCATTCCC CCAGACCCTC AGCAGCCACT GAGGCCCTGT 
GCCTCCCAGC CCTGCCTCTA TGGGGGCACG TGCTGGGACC AGGACTCTGG CCAAGGCTTC 
ACTTGCAGCT GCCCAGCGGG CAGGGGCGGC ATCATCTGCG AGGAGGTATT GCGCCCCTCT 
CTGCCTGCTT TTGGAGGCCA CTCCTTCCTG GCCTTCCCCA CCCTACGCGC CTACCACACA 
CTGCGCCTGG CACTAGAATT CCGAACACTG GAGCTGCAGG GGCTGCTGCT CTACAATGGG 
AACAACCGTG GCAAAGACTT CTTGGCGCTG GCTCTACAGG GTGGGCGGGT GCAGCTCAGG 
TTTGACACAG GTTCTGGGCC AGCAGTGTTG ACCAGTTCCG TGCCTGTGAA ACTGGGCCGG 
TGGCACCGCC TAGAACTGTC AAGGCATTGG CGCCAGGGCA CACTGTCTGT GGATGGCGAG 
AGCCCTGTTG TGGGCGAGAG CCCCAGTGGC ACGGATGGCC TTAACCTGGA CACGGACCTT 
TTTGTGGGTG GCGTCCCAGA GGACCAGGCT GCTGTGGTGC TTGAGCGGAC CTTCGTTGCA 
GTTGGCCTGA AAGGCTGCAT CCGGTTGCTG GACATCAACA ACCAGCGGCT GGAGCTGGGC 
AGCTGGCAAG GAGCTTCGAC CCAGAGCTCT GGCGTGGGCG AGTGTGGGGA AAACCCCTGT 
GTGCCCAACC CCTGCAGCAG TGGGGCGCCA TGCCAGGCAC TTGACGATGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCTG ACCTGTGCCA GTGAGAAGAA CCCCTGCCAG 
CCAAACCCTT GCCATGGAGC AGCCCCTTGC CGTGTACTAC CCGAGGGAGG GCCGAAGTGC 
GAGTGCCCCC TTGGGCGTGG GGGCGCCCTC TGCCAGACAG ACTTGGAGAA GGACCATGGC 
TCCCAGCCCT TCATGGCGGA TTTCAGCGGC TTCTCCTATC TGGAGCTGAA AGGCCTGTAC 
ACCTTTGAAA GAGACCTGGG AGAAAAGATG GCGCTGGAGG TTGTGTTCCT GGCCCGTGGC 
CCCAGTGGCT TGCTCCTTTA CAATGGGCAG AAGACAGATG GCAGAGGAGA CTTTGTGTCG 
CTGGCCCTGC GTGACTGGCA TCTGGAATTC CGTTATGACC TGGGCAAGGG CCCTGCAGTC 
ATCAGGAGCA AGGAGCCAGT CGCCGTGGAC ACCTGGACAA GGGTCTCACT GGAGCGCAAT 
GGCCGCAAGG GTGCCATGCG TGTGGGCGAT GGGCCCCGAG TGCTGGGGGA GTCCCCGAAA 
TCCCGCAAGG TGCCTCACAC TGTGCTCAAC CTGAAGGAGC CACTCTATGT GGGGGGCGCC 
CCTGATTTCA GTAGGTTGGC CCGGGCAGCT GCGGTGTCCT CTGGCTTTGA TGGTGCCATC 
CAGCTGGTTT CCCTGGGAGG CCGCCAGTTG CTGACCCAGG AACATGTGCT GCAGGCAGTG 
GGCATCTCCT CCTTTGCAGA CCACCCTTGC ACCCAAGCCT CGGGCCACCC CTGTCTTAAT 
GGAGCCTCGT GTCTCCCCCA GGAAGCCACC TATGAGTGCC TGTGTCCCTC AGGCTTCTCA 
GGGCTGCACT GTGAGAAGGG ACTGGTTGAA AAGTCGGCGG GGGACCTGGA CACATTGGCC 
TTTGATGGGC AGACCTACAT CGAGTACCTC AATGCTGTGA GGGAGAGCAA ACTGGCCAAT 
GAGATCCCAT TCCCGGAAGC TCCCGATTCC GGGTCCTTTC ACAGCGAGAA GGCGCTGCAG 
AGCAACCACT TCGAGCTGAG CCTGCGCACC GAGGCCACTC AGGGGCTGGT GCTGTGGAGC 
GGCAAGGCCA CAGAGCGTGC AGACTATGTG GCTCTGGCCA TCGTGGATGG GTACCTGCAG 
CTGAGCTACG ACCTGGGCTC CCAGCCCGTG GTGCTGCGCT CCACTGTGAA GGTCAACACC 
AATCACTGGC TGCGGGTTAA GGCTCACAGG GAGCAGAGGG AAGGGTCCCT TCAGGTGGGC 
AATGAAGCCC CTGTGACTGG CTCGTCTCCA CTGGGCTCCA CACAGCTGGA CACAGATGGA 
GCCCTGTGGC TCGGGGGCCT TCAGAAGCTG CCTGTGGGCC AGGCCCTGCC CAAGGCCTAC 
AGTACAGGCT TCGTGGGCTG CTTACGGGAT GTAGTGGTGA GCGGGCGCCC ACTGCACCTG 
CTGGAAGATG CAGTCACCAG GCCAGAGCTG CGGCCCTGCC CTGCACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008819.1
CDS	211..6081
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESKLANEIPFPEAPDSGSFHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153365.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153365.1

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGGAGC TGCTACAGGA GCCACGGCTG CAGTGGCCAC AGCCAGAGCC 
ACCACCACAG GCCAGCTGCC CTCTGCTGTA ACCCCTCGGG CCTACCCCAG TCACACCAGC 
CGACCTACTG GCAAGACCAC AGCACCCCTC ACCACACGCC GGCCTCTCAC TACGCTCCCC 
AGCCGCATGG CAGGACACCA ACCCATTCCC CCAGACCCTC AGCAGCCACT GAGGCCCTGT 
GCCTCCCAGC CCTGCCTCTA TGGGGGCACG TGCTGGGACC AGGACTCTGG CCAAGGCTTC 
ACTTGCAGCT GCCCAGCGGG CAGGGGCGGC ATCATCTGCG AGGAGGTATT GCGCCCCTCT 
CTGCCTGCTT TTGGAGGCCA CTCCTTCCTG GCCTTCCCCA CCCTACGCGC CTACCACACA 
CTGCGCCTGG CACTAGAATT CCGAACACTG GAGCTGCAGG GGCTGCTGCT CTACAATGGG 
AACAACCGTG GCAAAGACTT CTTGGCGCTG GCTCTACAGG GTGGGCGGGT GCAGCTCAGG 
TTTGACACAG GTTCTGGGCC AGCAGTGTTG ACCAGTTCCG TGCCTGTGAA ACTGGGCCGG 
TGGCACCGCC TAGAACTGTC AAGGCATTGG CGCCAGGGCA CACTGTCTGT GGATGGCGAG 
AGCCCTGTTG TGGGCGAGAG CCCCAGTGGC ACGGATGGCC TTAACCTGGA CACGGACCTT 
TTTGTGGGTG GCGTCCCAGA GGACCAGGCT GCTGTGGTGC TTGAGCGGAC CTTCGTTGCA 
GTTGGCCTGA AAGGCTGCAT CCGGTTGCTG GACATCAACA ACCAGCGGCT GGAGCTGGGC 
AGCTGGCAAG GAGCTTCGAC CCAGAGCTCT GGCGTGGGCG AGTGTGGGGA AAACCCCTGT 
GTGCCCAACC CCTGCAGCAG TGGGGCGCCA TGCCAGGCAC TTGACGATGG CATGTTCCAC 
TGTCAGTGCC CACCTGGCCG CTTTGGTCTG ACCTGTGCCA GTGAGAAGAA CCCCTGCCAG 
CCAAACCCTT GCCATGGAGC AGCCCCTTGC CGTGTACTAC CCGAGGGAGG GCCGAAGTGC 
GAGTGCCCCC TTGGGCGTGG GGGCGCCCTC TGCCAGACAG ACTTGGAGAA GGACCATGGC 
TCCCAGCCCT TCATGGCGGA TTTCAGCGGC TTCTCCTATC TGGAGCTGAA AGGCCTGTAC 
ACCTTTGAAA GAGACCTGGG AGAAAAGATG GCGCTGGAGG TTGTGTTCCT GGCCCGTGGC 
CCCAGTGGCT TGCTCCTTTA CAATGGGCAG AAGACAGATG GCAGAGGAGA CTTTGTGTCG 
CTGGCCCTGC GTGACTGGCA TCTGGAATTC CGTTATGACC TGGGCAAGGG CCCTGCAGTC 
ATCAGGAGCA AGGAGCCAGT CGCCGTGGAC ACCTGGACAA GGGTCTCACT GGAGCGCAAT 
GGCCGCAAGG GTGCCATGCG TGTGGGCGAT GGGCCCCGAG TGCTGGGGGA GTCCCCGAAA 
TCCCGCAAGG TGCCTCACAC TGTGCTCAAC CTGAAGGAGC CACTCTATGT GGGGGGCGCC 
CCTGATTTCA GTAGGTTGGC CCGGGCAGCT GCGGTGTCCT CTGGCTTTGA TGGTGCCATC 
CAGCTGGTTT CCCTGGGAGG CCGCCAGTTG CTGACCCAGG AACATGTGCT GCAGGCAGTG 
GGCATCTCCT CCTTTGCAGA CCACCCTTGC ACCCAAGCCT CGGGCCACCC CTGTCTTAAT 
GGAGCCTCGT GTCTCCCCCA GGAAGCCACC TATGAGTGCC TGTGTCCCTC AGGCTTCTCA 
GGGCTGCACT GTGAGAAGGG ACTGGTTGAA AAGTCGGCGG GGGACCTGGA CACATTGGCC 
TTTGATGGGC AGACCTACAT CGAGTACCTC AATGCTGTGA GGGAGAGCAA ACTGGCCAAT 
GAGATCCCAT TCCCGGAAGC TCCCGATTCC GGGTCCTTTC ACAGCGAGAA GGCGCTGCAG 
AGCAACCACT TCGAGCTGAG CCTGCGCACC GAGGCCACTC AGGGGCTGGT GCTGTGGAGC 
GGCAAGGCCA CAGAGCGTGC AGACTATGTG GCTCTGGCCA TCGTGGATGG GTACCTGCAG 
CTGAGCTACG ACCTGGGCTC CCAGCCCGTG GTGCTGCGCT CCACTGTGAA GGTCAACACC 
AATCACTGGC TGCGGGTTAA GGCTCACAGG GAGCAGAGGG AAGGGTCCCT TCAGGTGGGC 
AATGAAGCCC CTGTGACTGG CTCGTCTCCA CTGGGCTCCA CACAGCTGGA CACAGATGGA 
GCCCTGTGGC TCGGGGGCCT TCAGAAGCTG CCTGTGGGCC AGGCCCTGCC CAAGGCCTAC 
AGTACAGGCT TCGTGGGCTG CTTACGGGAT GTAGTGGTGA GCGGGCGCCC ACTGCACCTG 
CTGGAAGATG CAGTCACCAG GCCAGAGCTG CGGCCCTGCC CTGCACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_013153371.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008825.1 Cavia porcellus agrin (Agrn), transcript variant X6, mRNA.

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CloneID	ODo01584
Clone ID Related Accession (Same CDS sequence)	XM_013153371.2 , XM_013153371.1
Accession Version	XM_013153371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_013153371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCGAGAAGGC GCTGCAGAGC AACCACTTCG AGCTGAGCCT GCGCACCGAG 
GCCACTCAGG GGCTGGTGCT GTGGAGCGGC AAGGCCACAG AGCGTGCAGA CTATGTGGCT 
CTGGCCATCG TGGATGGGTA CCTGCAGCTG AGCTACGACC TGGGCTCCCA GCCCGTGGTG 
CTGCGCTCCA CTGTGAAGGT CAACACCAAT CACTGGCTGC GGGTTAAGGC TCACAGGGAG 
CAGAGGGAAG GGTCCCTTCA GGTGGGCAAT GAAGCCCCTG TGACTGGCTC GTCTCCACTG 
GGCTCCACAC AGCTGGACAC AGATGGAGCC CTGTGGCTCG GGGGCCTTCA GAAGCTGCCT 
GTGGGCCAGG CCCTGCCCAA GGCCTACAGT ACAGGCTTCG TGGGCTGCTT ACGGGATGTA 
GTGGTGAGCG GGCGCCCACT GCACCTGCTG GAAGATGCAG TCACCAGGCC AGAGCTGCGG 
CCCTGCCCTG CACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008825.1
CDS	186..6023
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153371.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153371.2

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCGAGAAGGC GCTGCAGAGC AACCACTTCG AGCTGAGCCT GCGCACCGAG 
GCCACTCAGG GGCTGGTGCT GTGGAGCGGC AAGGCCACAG AGCGTGCAGA CTATGTGGCT 
CTGGCCATCG TGGATGGGTA CCTGCAGCTG AGCTACGACC TGGGCTCCCA GCCCGTGGTG 
CTGCGCTCCA CTGTGAAGGT CAACACCAAT CACTGGCTGC GGGTTAAGGC TCACAGGGAG 
CAGAGGGAAG GGTCCCTTCA GGTGGGCAAT GAAGCCCCTG TGACTGGCTC GTCTCCACTG 
GGCTCCACAC AGCTGGACAC AGATGGAGCC CTGTGGCTCG GGGGCCTTCA GAAGCTGCCT 
GTGGGCCAGG CCCTGCCCAA GGCCTACAGT ACAGGCTTCG TGGGCTGCTT ACGGGATGTA 
GTGGTGAGCG GGCGCCCACT GCACCTGCTG GAAGATGCAG TCACCAGGCC AGAGCTGCGG 
CCCTGCCCTG CACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_013153371.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008825.1 Cavia porcellus agrin (Agrn), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODo01584
Clone ID Related Accession (Same CDS sequence)	XM_013153371.2 , XM_013153371.1
Accession Version	XM_013153371.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCGAGAAGGC GCTGCAGAGC AACCACTTCG AGCTGAGCCT GCGCACCGAG 
GCCACTCAGG GGCTGGTGCT GTGGAGCGGC AAGGCCACAG AGCGTGCAGA CTATGTGGCT 
CTGGCCATCG TGGATGGGTA CCTGCAGCTG AGCTACGACC TGGGCTCCCA GCCCGTGGTG 
CTGCGCTCCA CTGTGAAGGT CAACACCAAT CACTGGCTGC GGGTTAAGGC TCACAGGGAG 
CAGAGGGAAG GGTCCCTTCA GGTGGGCAAT GAAGCCCCTG TGACTGGCTC GTCTCCACTG 
GGCTCCACAC AGCTGGACAC AGATGGAGCC CTGTGGCTCG GGGGCCTTCA GAAGCTGCCT 
GTGGGCCAGG CCCTGCCCAA GGCCTACAGT ACAGGCTTCG TGGGCTGCTT ACGGGATGTA 
GTGGTGAGCG GGCGCCCACT GCACCTGCTG GAAGATGCAG TCACCAGGCC AGAGCTGCGG 
CCCTGCCCTG CACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008825.1
CDS	198..6035
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153371.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153371.1

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCGAGAAGGC GCTGCAGAGC AACCACTTCG AGCTGAGCCT GCGCACCGAG 
GCCACTCAGG GGCTGGTGCT GTGGAGCGGC AAGGCCACAG AGCGTGCAGA CTATGTGGCT 
CTGGCCATCG TGGATGGGTA CCTGCAGCTG AGCTACGACC TGGGCTCCCA GCCCGTGGTG 
CTGCGCTCCA CTGTGAAGGT CAACACCAAT CACTGGCTGC GGGTTAAGGC TCACAGGGAG 
CAGAGGGAAG GGTCCCTTCA GGTGGGCAAT GAAGCCCCTG TGACTGGCTC GTCTCCACTG 
GGCTCCACAC AGCTGGACAC AGATGGAGCC CTGTGGCTCG GGGGCCTTCA GAAGCTGCCT 
GTGGGCCAGG CCCTGCCCAA GGCCTACAGT ACAGGCTTCG TGGGCTGCTT ACGGGATGTA 
GTGGTGAGCG GGCGCCCACT GCACCTGCTG GAAGATGCAG TCACCAGGCC AGAGCTGCGG 
CCCTGCCCTG CACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_013153374.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008828.1 Cavia porcellus agrin (Agrn), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODo01585
Clone ID Related Accession (Same CDS sequence)	XM_013153374.2 , XM_013153374.1
Accession Version	XM_013153374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_013153374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
GAGAAGGCGC TGCAGAGCAA CCACTTCGAG CTGAGCCTGC GCACCGAGGC CACTCAGGGG 
CTGGTGCTGT GGAGCGGCAA GGCCACAGAG CGTGCAGACT ATGTGGCTCT GGCCATCGTG 
GATGGGTACC TGCAGCTGAG CTACGACCTG GGCTCCCAGC CCGTGGTGCT GCGCTCCACT 
GTGAAGGTCA ACACCAATCA CTGGCTGCGG GTTAAGGCTC ACAGGGAGCA GAGGGAAGGG 
TCCCTTCAGG TGGGCAATGA AGCCCCTGTG ACTGGCTCGT CTCCACTGGG CTCCACACAG 
CTGGACACAG ATGGAGCCCT GTGGCTCGGG GGCCTTCAGA AGCTGCCTGT GGGCCAGGCC 
CTGCCCAAGG CCTACAGTAC AGGCTTCGTG GGCTGCTTAC GGGATGTAGT GGTGAGCGGG 
CGCCCACTGC ACCTGCTGGA AGATGCAGTC ACCAGGCCAG AGCTGCGGCC CTGCCCTGCA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008828.1
CDS	186..6011
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153374.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153374.2

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
GAGAAGGCGC TGCAGAGCAA CCACTTCGAG CTGAGCCTGC GCACCGAGGC CACTCAGGGG 
CTGGTGCTGT GGAGCGGCAA GGCCACAGAG CGTGCAGACT ATGTGGCTCT GGCCATCGTG 
GATGGGTACC TGCAGCTGAG CTACGACCTG GGCTCCCAGC CCGTGGTGCT GCGCTCCACT 
GTGAAGGTCA ACACCAATCA CTGGCTGCGG GTTAAGGCTC ACAGGGAGCA GAGGGAAGGG 
TCCCTTCAGG TGGGCAATGA AGCCCCTGTG ACTGGCTCGT CTCCACTGGG CTCCACACAG 
CTGGACACAG ATGGAGCCCT GTGGCTCGGG GGCCTTCAGA AGCTGCCTGT GGGCCAGGCC 
CTGCCCAAGG CCTACAGTAC AGGCTTCGTG GGCTGCTTAC GGGATGTAGT GGTGAGCGGG 
CGCCCACTGC ACCTGCTGGA AGATGCAGTC ACCAGGCCAG AGCTGCGGCC CTGCCCTGCA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_013153374.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008828.1 Cavia porcellus agrin (Agrn), transcript variant X5, mRNA.

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CloneID	ODo01585
Clone ID Related Accession (Same CDS sequence)	XM_013153374.2 , XM_013153374.1
Accession Version	XM_013153374.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
GAGAAGGCGC TGCAGAGCAA CCACTTCGAG CTGAGCCTGC GCACCGAGGC CACTCAGGGG 
CTGGTGCTGT GGAGCGGCAA GGCCACAGAG CGTGCAGACT ATGTGGCTCT GGCCATCGTG 
GATGGGTACC TGCAGCTGAG CTACGACCTG GGCTCCCAGC CCGTGGTGCT GCGCTCCACT 
GTGAAGGTCA ACACCAATCA CTGGCTGCGG GTTAAGGCTC ACAGGGAGCA GAGGGAAGGG 
TCCCTTCAGG TGGGCAATGA AGCCCCTGTG ACTGGCTCGT CTCCACTGGG CTCCACACAG 
CTGGACACAG ATGGAGCCCT GTGGCTCGGG GGCCTTCAGA AGCTGCCTGT GGGCCAGGCC 
CTGCCCAAGG CCTACAGTAC AGGCTTCGTG GGCTGCTTAC GGGATGTAGT GGTGAGCGGG 
CGCCCACTGC ACCTGCTGGA AGATGCAGTC ACCAGGCCAG AGCTGCGGCC CTGCCCTGCA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008828.1
CDS	196..6021
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153374.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153374.1

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GGTGCCTCAC ACTGTGCTCA ACCTGAAGGA GCCACTCTAT 
GTGGGGGGCG CCCCTGATTT CAGTAGGTTG GCCCGGGCAG CTGCGGTGTC CTCTGGCTTT 
GATGGTGCCA TCCAGCTGGT TTCCCTGGGA GGCCGCCAGT TGCTGACCCA GGAACATGTG 
CTGCAGGCAG TGGGCATCTC CTCCTTTGCA GACCACCCTT GCACCCAAGC CTCGGGCCAC 
CCCTGTCTTA ATGGAGCCTC GTGTCTCCCC CAGGAAGCCA CCTATGAGTG CCTGTGTCCC 
TCAGGCTTCT CAGGGCTGCA CTGTGAGAAG GGACTGGTTG AAAAGTCGGC GGGGGACCTG 
GACACATTGG CCTTTGATGG GCAGACCTAC ATCGAGTACC TCAATGCTGT GAGGGAGAGC 
GAGAAGGCGC TGCAGAGCAA CCACTTCGAG CTGAGCCTGC GCACCGAGGC CACTCAGGGG 
CTGGTGCTGT GGAGCGGCAA GGCCACAGAG CGTGCAGACT ATGTGGCTCT GGCCATCGTG 
GATGGGTACC TGCAGCTGAG CTACGACCTG GGCTCCCAGC CCGTGGTGCT GCGCTCCACT 
GTGAAGGTCA ACACCAATCA CTGGCTGCGG GTTAAGGCTC ACAGGGAGCA GAGGGAAGGG 
TCCCTTCAGG TGGGCAATGA AGCCCCTGTG ACTGGCTCGT CTCCACTGGG CTCCACACAG 
CTGGACACAG ATGGAGCCCT GTGGCTCGGG GGCCTTCAGA AGCTGCCTGT GGGCCAGGCC 
CTGCCCAAGG CCTACAGTAC AGGCTTCGTG GGCTGCTTAC GGGATGTAGT GGTGAGCGGG 
CGCCCACTGC ACCTGCTGGA AGATGCAGTC ACCAGGCCAG AGCTGCGGCC CTGCCCTGCA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_013153376.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008830.1 Cavia porcellus agrin (Agrn), transcript variant X8, mRNA.

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CloneID	ODo01586
Clone ID Related Accession (Same CDS sequence)	XM_013153376.2 , XM_013153376.1
Accession Version	XM_013153376.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_013153376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGA CCAGTGTGGA 
CAGTGTCGTT TTGGAGCCCT GTGTGAGGCC GAAACCGGGC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGT TCTGATGGGC ACACATATGC CAGTGAATGT 
GAGCTTCATG TCCACGCCTG TACACAGCAG GTTGATCTGC ACGTGATCTC AGCTGGACCC 
TGCCAGACCT GTAAGGATAC AGTGTGTGCC TTTGGGGCAG TGTGCTCAGC TGGGCAGTGC 
GTGTGTCCTC GGTGTGAGCA CCCCCCACCA GGCCCCGTGT GCGGCAGTGA TGGTATCACC 
TACCCCAGTG CATGTGAGCT GCGGGAAGCT GCCTGCCGGC AGCAGGTACA AATTGAAGAA 
GCCCGGGCAG GACCCTGTGA GCAAGCTGAA TGTGGCTCAG GAGGCTCTGG CTCTGGAGAG 
GAAGGCGAGT GTGAGCGGGA ACTGTGCCAG CAGCGTGGTG GCATCTGGGA TGAGGACTCA 
GAGGATGGGC CATGTGTCTG TGACTTCAGC TGTCAGAGTG TCCTGAGGAG CCCGGTATGC 
GGCTCAGATG GGGTTACCTA TGGCACTGAG TGTGACCTGA AGAAGGCCAG GTGTGAATCA 
CAACGAGAAC TGTACGTCGC GGCTCAGGGG CCCTGCCACA GTCCTACATT GGCACCACTG 
CCACCTGTGT TTCTTTCACA CTGTGCCCAG ACCCCATATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCA CCTGCCAGTG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGCGTG 
GGAGGCCTTA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ATTTCCGTGG CATTGTCACT 
GACAACCTGA GTGGCTGTAC TCCCTGCAGC TGTGACCCCC AGGGTGCTGT GCGGGACGAC 
TGTGAGCAGA TGACTGGACT GTGCTCCTGC AAGCCAGGAG TGGCTGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCAAAC CCTGGGCCCT ACTGGTTGTG AAGCAGGCTC CTTGATGCCC 
AAGACCTGTG TGGAGATGCA CTGTGAGTTT GGAGCTTCCT GTGTGGAAGA AGCTGGTTCT 
GCCCGCTGTG CCTGCCCAGC CCTCACGTGC CCAGAGGCCA ATGCCACTAA GGTCTGTGGG 
TCAGATGGTA TCACATACGG CAATGAGTGC CAGCTGAAGA CGATTGCCTG CCGCCAGGGC 
CTGGACATCT TCACCCAGAG TGTTGGCCCA TGCCAGGATG CTGTTGCTCT TGGCGTATAC 
CCCACATCTG TATCCATGAC CACCCTGGAG CATGTCCTGA ATGAGGCGCT GCCGTCCCCC 
TCCAGTGCCC TTTCCCTGGC ACCCAGCAGT ATTCCCCGCA GCCAGGCTAC TCCTCTACCC 
ACATCACGAC CCTGGACCAC ATCCAGCATC CCCAGGCCCA CGGCAAGACC TGGGCCTGTG 
CTAACCATGC CCCCCACAAC CACCTCCACC ACTGCCAGCC TTCCCGCATC AGCCTTCGGT 
GAATCTGGCA GCACCAATGG GAGTGGTGAT GAGGAGCTCA GCGGAGACCA GGAGGCCAGT 
GGGGGAGGCT CTGGGGGACT TGAGCTCCCC ATGGGCAACA ACATTGTGAC TGCTGGGCCA 
CCCATCGAGA GGGCTTCCTG CTACAACTCT CCTTTGGGCT GCTGTTCAGA TGGCAAGACA 
CCCTCACTGG ATGCAGAGAG CTCCAACTGC CCTGCCACCA AGGCGTTCCA GGGCGTACTG 
GAGCTGGAGG GGGTCGAGGG GCAGGAGCTG TTCTACACAC CTGAGATGGC TGACCCCAAG 
TCAGAACTGT TTGGGGAAAC TGCCAGGAGC ATTGAGAACT CGCTGGATGA CCTGTTCCGA 
AACTCAGATG TCAAGAAGGA CTTCCGGAGT GTCCGCCTGC GGGACCTGGG GCCTGGCAGA 
TCGGTCCGAG CTATTGTGGA TGTGCACTTT GACCCCACTA CGGCCTTCAG GGCTCCCGAT 
GTGGGCCAGG CCCTCCTCCG GCAGATCCAG GCATCCAGAC GCCGGACCTT ACTGGTGAGG 
AGGCCTCTTC ATGAACATGT GCGATTCATG GACTTTGACT GGTTTCCAGC CTTCTTCACA 
GGAGCTGCTA CAGGAGCCAC GGCTGCAGTG GCCACAGCCA GAGCCACCAC CACAGGCCAG 
CTGCCCTCTG CTGTAACCCC TCGGGCCTAC CCCAGTCACA CCAGCCGACC TACTGGCAAG 
ACCACAGCAC CCCTCACCAC ACGCCGGCCT CTCACTACGC TCCCCAGCCG CATGGCAGGA 
CACCAACCCA TTCCCCCAGA CCCTCAGCAG CCACTGAGGC CCTGTGCCTC CCAGCCCTGC 
CTCTATGGGG GCACGTGCTG GGACCAGGAC TCTGGCCAAG GCTTCACTTG CAGCTGCCCA 
GCGGGCAGGG GCGGCATCAT CTGCGAGGAG GTATTGCGCC CCTCTCTGCC TGCTTTTGGA 
GGCCACTCCT TCCTGGCCTT CCCCACCCTA CGCGCCTACC ACACACTGCG CCTGGCACTA 
GAATTCCGAA CACTGGAGCT GCAGGGGCTG CTGCTCTACA ATGGGAACAA CCGTGGCAAA 
GACTTCTTGG CGCTGGCTCT ACAGGGTGGG CGGGTGCAGC TCAGGTTTGA CACAGGTTCT 
GGGCCAGCAG TGTTGACCAG TTCCGTGCCT GTGAAACTGG GCCGGTGGCA CCGCCTAGAA 
CTGTCAAGGC ATTGGCGCCA GGGCACACTG TCTGTGGATG GCGAGAGCCC TGTTGTGGGC 
GAGAGCCCCA GTGGCACGGA TGGCCTTAAC CTGGACACGG ACCTTTTTGT GGGTGGCGTC 
CCAGAGGACC AGGCTGCTGT GGTGCTTGAG CGGACCTTCG TTGCAGTTGG CCTGAAAGGC 
TGCATCCGGT TGCTGGACAT CAACAACCAG CGGCTGGAGC TGGGCAGCTG GCAAGGAGCT 
TCGACCCAGA GCTCTGGCGT GGGCGAGTGT GGGGAAAACC CCTGTGTGCC CAACCCCTGC 
AGCAGTGGGG CGCCATGCCA GGCACTTGAC GATGGCATGT TCCACTGTCA GTGCCCACCT 
GGCCGCTTTG GTCTGACCTG TGCCAGTGAG AAGAACCCCT GCCAGCCAAA CCCTTGCCAT 
GGAGCAGCCC CTTGCCGTGT ACTACCCGAG GGAGGGCCGA AGTGCGAGTG CCCCCTTGGG 
CGTGGGGGCG CCCTCTGCCA GACAGACTTG GAGAAGGACC ATGGCTCCCA GCCCTTCATG 
GCGGATTTCA GCGGCTTCTC CTATCTGGAG CTGAAAGGCC TGTACACCTT TGAAAGAGAC 
CTGGGAGAAA AGATGGCGCT GGAGGTTGTG TTCCTGGCCC GTGGCCCCAG TGGCTTGCTC 
CTTTACAATG GGCAGAAGAC AGATGGCAGA GGAGACTTTG TGTCGCTGGC CCTGCGTGAC 
TGGCATCTGG AATTCCGTTA TGACCTGGGC AAGGGCCCTG CAGTCATCAG GAGCAAGGAG 
CCAGTCGCCG TGGACACCTG GACAAGGGTC TCACTGGAGC GCAATGGCCG CAAGGGTGCC 
ATGCGTGTGG GCGATGGGCC CCGAGTGCTG GGGGAGTCCC CGAAATCCCG CAAGGTGCCT 
CACACTGTGC TCAACCTGAA GGAGCCACTC TATGTGGGGG GCGCCCCTGA TTTCAGTAGG 
TTGGCCCGGG CAGCTGCGGT GTCCTCTGGC TTTGATGGTG CCATCCAGCT GGTTTCCCTG 
GGAGGCCGCC AGTTGCTGAC CCAGGAACAT GTGCTGCAGG CAGTGGGCAT CTCCTCCTTT 
GCAGACCACC CTTGCACCCA AGCCTCGGGC CACCCCTGTC TTAATGGAGC CTCGTGTCTC 
CCCCAGGAAG CCACCTATGA GTGCCTGTGT CCCTCAGGCT TCTCAGGGCT GCACTGTGAG 
AAGGGACTGG TTGAAAAGTC GGCGGGGGAC CTGGACACAT TGGCCTTTGA TGGGCAGACC 
TACATCGAGT ACCTCAATGC TGTGAGGGAG AGCAAACTGG CCAATGAGAT CCCATTCCCG 
GAAGCTCCCG ATTCCGGGTC CTTTCACAGC GAGAAGGCGC TGCAGAGCAA CCACTTCGAG 
CTGAGCCTGC GCACCGAGGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACAGAG 
CGTGCAGACT ATGTGGCTCT GGCCATCGTG GATGGGTACC TGCAGCTGAG CTACGACCTG 
GGCTCCCAGC CCGTGGTGCT GCGCTCCACT GTGAAGGTCA ACACCAATCA CTGGCTGCGG 
GTTAAGGCTC ACAGGGAGCA GAGGGAAGGG TCCCTTCAGG TGGGCAATGA AGCCCCTGTG 
ACTGGCTCGT CTCCACTGGG CTCCACACAG CTGGACACAG ATGGAGCCCT GTGGCTCGGG 
GGCCTTCAGA AGCTGCCTGT GGGCCAGGCC CTGCCCAAGG CCTACAGTAC AGGCTTCGTG 
GGCTGCTTAC GGGATGTAGT GGTGAGCGGG CGCCCACTGC ACCTGCTGGA AGATGCAGTC 
ACCAGGCCAG AGCTGCGGCC CTGCCCTGCA CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008830.1
CDS	188..5863
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESKLANEIPFPEAPDSGSFHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153376.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153376.2

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGA CCAGTGTGGA 
CAGTGTCGTT TTGGAGCCCT GTGTGAGGCC GAAACCGGGC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGT TCTGATGGGC ACACATATGC CAGTGAATGT 
GAGCTTCATG TCCACGCCTG TACACAGCAG GTTGATCTGC ACGTGATCTC AGCTGGACCC 
TGCCAGACCT GTAAGGATAC AGTGTGTGCC TTTGGGGCAG TGTGCTCAGC TGGGCAGTGC 
GTGTGTCCTC GGTGTGAGCA CCCCCCACCA GGCCCCGTGT GCGGCAGTGA TGGTATCACC 
TACCCCAGTG CATGTGAGCT GCGGGAAGCT GCCTGCCGGC AGCAGGTACA AATTGAAGAA 
GCCCGGGCAG GACCCTGTGA GCAAGCTGAA TGTGGCTCAG GAGGCTCTGG CTCTGGAGAG 
GAAGGCGAGT GTGAGCGGGA ACTGTGCCAG CAGCGTGGTG GCATCTGGGA TGAGGACTCA 
GAGGATGGGC CATGTGTCTG TGACTTCAGC TGTCAGAGTG TCCTGAGGAG CCCGGTATGC 
GGCTCAGATG GGGTTACCTA TGGCACTGAG TGTGACCTGA AGAAGGCCAG GTGTGAATCA 
CAACGAGAAC TGTACGTCGC GGCTCAGGGG CCCTGCCACA GTCCTACATT GGCACCACTG 
CCACCTGTGT TTCTTTCACA CTGTGCCCAG ACCCCATATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCA CCTGCCAGTG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGCGTG 
GGAGGCCTTA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ATTTCCGTGG CATTGTCACT 
GACAACCTGA GTGGCTGTAC TCCCTGCAGC TGTGACCCCC AGGGTGCTGT GCGGGACGAC 
TGTGAGCAGA TGACTGGACT GTGCTCCTGC AAGCCAGGAG TGGCTGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCAAAC CCTGGGCCCT ACTGGTTGTG AAGCAGGCTC CTTGATGCCC 
AAGACCTGTG TGGAGATGCA CTGTGAGTTT GGAGCTTCCT GTGTGGAAGA AGCTGGTTCT 
GCCCGCTGTG CCTGCCCAGC CCTCACGTGC CCAGAGGCCA ATGCCACTAA GGTCTGTGGG 
TCAGATGGTA TCACATACGG CAATGAGTGC CAGCTGAAGA CGATTGCCTG CCGCCAGGGC 
CTGGACATCT TCACCCAGAG TGTTGGCCCA TGCCAGGATG CTGTTGCTCT TGGCGTATAC 
CCCACATCTG TATCCATGAC CACCCTGGAG CATGTCCTGA ATGAGGCGCT GCCGTCCCCC 
TCCAGTGCCC TTTCCCTGGC ACCCAGCAGT ATTCCCCGCA GCCAGGCTAC TCCTCTACCC 
ACATCACGAC CCTGGACCAC ATCCAGCATC CCCAGGCCCA CGGCAAGACC TGGGCCTGTG 
CTAACCATGC CCCCCACAAC CACCTCCACC ACTGCCAGCC TTCCCGCATC AGCCTTCGGT 
GAATCTGGCA GCACCAATGG GAGTGGTGAT GAGGAGCTCA GCGGAGACCA GGAGGCCAGT 
GGGGGAGGCT CTGGGGGACT TGAGCTCCCC ATGGGCAACA ACATTGTGAC TGCTGGGCCA 
CCCATCGAGA GGGCTTCCTG CTACAACTCT CCTTTGGGCT GCTGTTCAGA TGGCAAGACA 
CCCTCACTGG ATGCAGAGAG CTCCAACTGC CCTGCCACCA AGGCGTTCCA GGGCGTACTG 
GAGCTGGAGG GGGTCGAGGG GCAGGAGCTG TTCTACACAC CTGAGATGGC TGACCCCAAG 
TCAGAACTGT TTGGGGAAAC TGCCAGGAGC ATTGAGAACT CGCTGGATGA CCTGTTCCGA 
AACTCAGATG TCAAGAAGGA CTTCCGGAGT GTCCGCCTGC GGGACCTGGG GCCTGGCAGA 
TCGGTCCGAG CTATTGTGGA TGTGCACTTT GACCCCACTA CGGCCTTCAG GGCTCCCGAT 
GTGGGCCAGG CCCTCCTCCG GCAGATCCAG GCATCCAGAC GCCGGACCTT ACTGGTGAGG 
AGGCCTCTTC ATGAACATGT GCGATTCATG GACTTTGACT GGTTTCCAGC CTTCTTCACA 
GGAGCTGCTA CAGGAGCCAC GGCTGCAGTG GCCACAGCCA GAGCCACCAC CACAGGCCAG 
CTGCCCTCTG CTGTAACCCC TCGGGCCTAC CCCAGTCACA CCAGCCGACC TACTGGCAAG 
ACCACAGCAC CCCTCACCAC ACGCCGGCCT CTCACTACGC TCCCCAGCCG CATGGCAGGA 
CACCAACCCA TTCCCCCAGA CCCTCAGCAG CCACTGAGGC CCTGTGCCTC CCAGCCCTGC 
CTCTATGGGG GCACGTGCTG GGACCAGGAC TCTGGCCAAG GCTTCACTTG CAGCTGCCCA 
GCGGGCAGGG GCGGCATCAT CTGCGAGGAG GTATTGCGCC CCTCTCTGCC TGCTTTTGGA 
GGCCACTCCT TCCTGGCCTT CCCCACCCTA CGCGCCTACC ACACACTGCG CCTGGCACTA 
GAATTCCGAA CACTGGAGCT GCAGGGGCTG CTGCTCTACA ATGGGAACAA CCGTGGCAAA 
GACTTCTTGG CGCTGGCTCT ACAGGGTGGG CGGGTGCAGC TCAGGTTTGA CACAGGTTCT 
GGGCCAGCAG TGTTGACCAG TTCCGTGCCT GTGAAACTGG GCCGGTGGCA CCGCCTAGAA 
CTGTCAAGGC ATTGGCGCCA GGGCACACTG TCTGTGGATG GCGAGAGCCC TGTTGTGGGC 
GAGAGCCCCA GTGGCACGGA TGGCCTTAAC CTGGACACGG ACCTTTTTGT GGGTGGCGTC 
CCAGAGGACC AGGCTGCTGT GGTGCTTGAG CGGACCTTCG TTGCAGTTGG CCTGAAAGGC 
TGCATCCGGT TGCTGGACAT CAACAACCAG CGGCTGGAGC TGGGCAGCTG GCAAGGAGCT 
TCGACCCAGA GCTCTGGCGT GGGCGAGTGT GGGGAAAACC CCTGTGTGCC CAACCCCTGC 
AGCAGTGGGG CGCCATGCCA GGCACTTGAC GATGGCATGT TCCACTGTCA GTGCCCACCT 
GGCCGCTTTG GTCTGACCTG TGCCAGTGAG AAGAACCCCT GCCAGCCAAA CCCTTGCCAT 
GGAGCAGCCC CTTGCCGTGT ACTACCCGAG GGAGGGCCGA AGTGCGAGTG CCCCCTTGGG 
CGTGGGGGCG CCCTCTGCCA GACAGACTTG GAGAAGGACC ATGGCTCCCA GCCCTTCATG 
GCGGATTTCA GCGGCTTCTC CTATCTGGAG CTGAAAGGCC TGTACACCTT TGAAAGAGAC 
CTGGGAGAAA AGATGGCGCT GGAGGTTGTG TTCCTGGCCC GTGGCCCCAG TGGCTTGCTC 
CTTTACAATG GGCAGAAGAC AGATGGCAGA GGAGACTTTG TGTCGCTGGC CCTGCGTGAC 
TGGCATCTGG AATTCCGTTA TGACCTGGGC AAGGGCCCTG CAGTCATCAG GAGCAAGGAG 
CCAGTCGCCG TGGACACCTG GACAAGGGTC TCACTGGAGC GCAATGGCCG CAAGGGTGCC 
ATGCGTGTGG GCGATGGGCC CCGAGTGCTG GGGGAGTCCC CGAAATCCCG CAAGGTGCCT 
CACACTGTGC TCAACCTGAA GGAGCCACTC TATGTGGGGG GCGCCCCTGA TTTCAGTAGG 
TTGGCCCGGG CAGCTGCGGT GTCCTCTGGC TTTGATGGTG CCATCCAGCT GGTTTCCCTG 
GGAGGCCGCC AGTTGCTGAC CCAGGAACAT GTGCTGCAGG CAGTGGGCAT CTCCTCCTTT 
GCAGACCACC CTTGCACCCA AGCCTCGGGC CACCCCTGTC TTAATGGAGC CTCGTGTCTC 
CCCCAGGAAG CCACCTATGA GTGCCTGTGT CCCTCAGGCT TCTCAGGGCT GCACTGTGAG 
AAGGGACTGG TTGAAAAGTC GGCGGGGGAC CTGGACACAT TGGCCTTTGA TGGGCAGACC 
TACATCGAGT ACCTCAATGC TGTGAGGGAG AGCAAACTGG CCAATGAGAT CCCATTCCCG 
GAAGCTCCCG ATTCCGGGTC CTTTCACAGC GAGAAGGCGC TGCAGAGCAA CCACTTCGAG 
CTGAGCCTGC GCACCGAGGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACAGAG 
CGTGCAGACT ATGTGGCTCT GGCCATCGTG GATGGGTACC TGCAGCTGAG CTACGACCTG 
GGCTCCCAGC CCGTGGTGCT GCGCTCCACT GTGAAGGTCA ACACCAATCA CTGGCTGCGG 
GTTAAGGCTC ACAGGGAGCA GAGGGAAGGG TCCCTTCAGG TGGGCAATGA AGCCCCTGTG 
ACTGGCTCGT CTCCACTGGG CTCCACACAG CTGGACACAG ATGGAGCCCT GTGGCTCGGG 
GGCCTTCAGA AGCTGCCTGT GGGCCAGGCC CTGCCCAAGG CCTACAGTAC AGGCTTCGTG 
GGCTGCTTAC GGGATGTAGT GGTGAGCGGG CGCCCACTGC ACCTGCTGGA AGATGCAGTC 
ACCAGGCCAG AGCTGCGGCC CTGCCCTGCA CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_013153376.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008830.1 Cavia porcellus agrin (Agrn), transcript variant X6, mRNA.

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CloneID	ODo01586
Clone ID Related Accession (Same CDS sequence)	XM_013153376.2 , XM_013153376.1
Accession Version	XM_013153376.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGA CCAGTGTGGA 
CAGTGTCGTT TTGGAGCCCT GTGTGAGGCC GAAACCGGGC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGT TCTGATGGGC ACACATATGC CAGTGAATGT 
GAGCTTCATG TCCACGCCTG TACACAGCAG GTTGATCTGC ACGTGATCTC AGCTGGACCC 
TGCCAGACCT GTAAGGATAC AGTGTGTGCC TTTGGGGCAG TGTGCTCAGC TGGGCAGTGC 
GTGTGTCCTC GGTGTGAGCA CCCCCCACCA GGCCCCGTGT GCGGCAGTGA TGGTATCACC 
TACCCCAGTG CATGTGAGCT GCGGGAAGCT GCCTGCCGGC AGCAGGTACA AATTGAAGAA 
GCCCGGGCAG GACCCTGTGA GCAAGCTGAA TGTGGCTCAG GAGGCTCTGG CTCTGGAGAG 
GAAGGCGAGT GTGAGCGGGA ACTGTGCCAG CAGCGTGGTG GCATCTGGGA TGAGGACTCA 
GAGGATGGGC CATGTGTCTG TGACTTCAGC TGTCAGAGTG TCCTGAGGAG CCCGGTATGC 
GGCTCAGATG GGGTTACCTA TGGCACTGAG TGTGACCTGA AGAAGGCCAG GTGTGAATCA 
CAACGAGAAC TGTACGTCGC GGCTCAGGGG CCCTGCCACA GTCCTACATT GGCACCACTG 
CCACCTGTGT TTCTTTCACA CTGTGCCCAG ACCCCATATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCA CCTGCCAGTG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGCGTG 
GGAGGCCTTA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ATTTCCGTGG CATTGTCACT 
GACAACCTGA GTGGCTGTAC TCCCTGCAGC TGTGACCCCC AGGGTGCTGT GCGGGACGAC 
TGTGAGCAGA TGACTGGACT GTGCTCCTGC AAGCCAGGAG TGGCTGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCAAAC CCTGGGCCCT ACTGGTTGTG AAGCAGGCTC CTTGATGCCC 
AAGACCTGTG TGGAGATGCA CTGTGAGTTT GGAGCTTCCT GTGTGGAAGA AGCTGGTTCT 
GCCCGCTGTG CCTGCCCAGC CCTCACGTGC CCAGAGGCCA ATGCCACTAA GGTCTGTGGG 
TCAGATGGTA TCACATACGG CAATGAGTGC CAGCTGAAGA CGATTGCCTG CCGCCAGGGC 
CTGGACATCT TCACCCAGAG TGTTGGCCCA TGCCAGGATG CTGTTGCTCT TGGCGTATAC 
CCCACATCTG TATCCATGAC CACCCTGGAG CATGTCCTGA ATGAGGCGCT GCCGTCCCCC 
TCCAGTGCCC TTTCCCTGGC ACCCAGCAGT ATTCCCCGCA GCCAGGCTAC TCCTCTACCC 
ACATCACGAC CCTGGACCAC ATCCAGCATC CCCAGGCCCA CGGCAAGACC TGGGCCTGTG 
CTAACCATGC CCCCCACAAC CACCTCCACC ACTGCCAGCC TTCCCGCATC AGCCTTCGGT 
GAATCTGGCA GCACCAATGG GAGTGGTGAT GAGGAGCTCA GCGGAGACCA GGAGGCCAGT 
GGGGGAGGCT CTGGGGGACT TGAGCTCCCC ATGGGCAACA ACATTGTGAC TGCTGGGCCA 
CCCATCGAGA GGGCTTCCTG CTACAACTCT CCTTTGGGCT GCTGTTCAGA TGGCAAGACA 
CCCTCACTGG ATGCAGAGAG CTCCAACTGC CCTGCCACCA AGGCGTTCCA GGGCGTACTG 
GAGCTGGAGG GGGTCGAGGG GCAGGAGCTG TTCTACACAC CTGAGATGGC TGACCCCAAG 
TCAGAACTGT TTGGGGAAAC TGCCAGGAGC ATTGAGAACT CGCTGGATGA CCTGTTCCGA 
AACTCAGATG TCAAGAAGGA CTTCCGGAGT GTCCGCCTGC GGGACCTGGG GCCTGGCAGA 
TCGGTCCGAG CTATTGTGGA TGTGCACTTT GACCCCACTA CGGCCTTCAG GGCTCCCGAT 
GTGGGCCAGG CCCTCCTCCG GCAGATCCAG GCATCCAGAC GCCGGACCTT ACTGGTGAGG 
AGGCCTCTTC ATGAACATGT GCGATTCATG GACTTTGACT GGTTTCCAGC CTTCTTCACA 
GGAGCTGCTA CAGGAGCCAC GGCTGCAGTG GCCACAGCCA GAGCCACCAC CACAGGCCAG 
CTGCCCTCTG CTGTAACCCC TCGGGCCTAC CCCAGTCACA CCAGCCGACC TACTGGCAAG 
ACCACAGCAC CCCTCACCAC ACGCCGGCCT CTCACTACGC TCCCCAGCCG CATGGCAGGA 
CACCAACCCA TTCCCCCAGA CCCTCAGCAG CCACTGAGGC CCTGTGCCTC CCAGCCCTGC 
CTCTATGGGG GCACGTGCTG GGACCAGGAC TCTGGCCAAG GCTTCACTTG CAGCTGCCCA 
GCGGGCAGGG GCGGCATCAT CTGCGAGGAG GTATTGCGCC CCTCTCTGCC TGCTTTTGGA 
GGCCACTCCT TCCTGGCCTT CCCCACCCTA CGCGCCTACC ACACACTGCG CCTGGCACTA 
GAATTCCGAA CACTGGAGCT GCAGGGGCTG CTGCTCTACA ATGGGAACAA CCGTGGCAAA 
GACTTCTTGG CGCTGGCTCT ACAGGGTGGG CGGGTGCAGC TCAGGTTTGA CACAGGTTCT 
GGGCCAGCAG TGTTGACCAG TTCCGTGCCT GTGAAACTGG GCCGGTGGCA CCGCCTAGAA 
CTGTCAAGGC ATTGGCGCCA GGGCACACTG TCTGTGGATG GCGAGAGCCC TGTTGTGGGC 
GAGAGCCCCA GTGGCACGGA TGGCCTTAAC CTGGACACGG ACCTTTTTGT GGGTGGCGTC 
CCAGAGGACC AGGCTGCTGT GGTGCTTGAG CGGACCTTCG TTGCAGTTGG CCTGAAAGGC 
TGCATCCGGT TGCTGGACAT CAACAACCAG CGGCTGGAGC TGGGCAGCTG GCAAGGAGCT 
TCGACCCAGA GCTCTGGCGT GGGCGAGTGT GGGGAAAACC CCTGTGTGCC CAACCCCTGC 
AGCAGTGGGG CGCCATGCCA GGCACTTGAC GATGGCATGT TCCACTGTCA GTGCCCACCT 
GGCCGCTTTG GTCTGACCTG TGCCAGTGAG AAGAACCCCT GCCAGCCAAA CCCTTGCCAT 
GGAGCAGCCC CTTGCCGTGT ACTACCCGAG GGAGGGCCGA AGTGCGAGTG CCCCCTTGGG 
CGTGGGGGCG CCCTCTGCCA GACAGACTTG GAGAAGGACC ATGGCTCCCA GCCCTTCATG 
GCGGATTTCA GCGGCTTCTC CTATCTGGAG CTGAAAGGCC TGTACACCTT TGAAAGAGAC 
CTGGGAGAAA AGATGGCGCT GGAGGTTGTG TTCCTGGCCC GTGGCCCCAG TGGCTTGCTC 
CTTTACAATG GGCAGAAGAC AGATGGCAGA GGAGACTTTG TGTCGCTGGC CCTGCGTGAC 
TGGCATCTGG AATTCCGTTA TGACCTGGGC AAGGGCCCTG CAGTCATCAG GAGCAAGGAG 
CCAGTCGCCG TGGACACCTG GACAAGGGTC TCACTGGAGC GCAATGGCCG CAAGGGTGCC 
ATGCGTGTGG GCGATGGGCC CCGAGTGCTG GGGGAGTCCC CGAAATCCCG CAAGGTGCCT 
CACACTGTGC TCAACCTGAA GGAGCCACTC TATGTGGGGG GCGCCCCTGA TTTCAGTAGG 
TTGGCCCGGG CAGCTGCGGT GTCCTCTGGC TTTGATGGTG CCATCCAGCT GGTTTCCCTG 
GGAGGCCGCC AGTTGCTGAC CCAGGAACAT GTGCTGCAGG CAGTGGGCAT CTCCTCCTTT 
GCAGACCACC CTTGCACCCA AGCCTCGGGC CACCCCTGTC TTAATGGAGC CTCGTGTCTC 
CCCCAGGAAG CCACCTATGA GTGCCTGTGT CCCTCAGGCT TCTCAGGGCT GCACTGTGAG 
AAGGGACTGG TTGAAAAGTC GGCGGGGGAC CTGGACACAT TGGCCTTTGA TGGGCAGACC 
TACATCGAGT ACCTCAATGC TGTGAGGGAG AGCAAACTGG CCAATGAGAT CCCATTCCCG 
GAAGCTCCCG ATTCCGGGTC CTTTCACAGC GAGAAGGCGC TGCAGAGCAA CCACTTCGAG 
CTGAGCCTGC GCACCGAGGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACAGAG 
CGTGCAGACT ATGTGGCTCT GGCCATCGTG GATGGGTACC TGCAGCTGAG CTACGACCTG 
GGCTCCCAGC CCGTGGTGCT GCGCTCCACT GTGAAGGTCA ACACCAATCA CTGGCTGCGG 
GTTAAGGCTC ACAGGGAGCA GAGGGAAGGG TCCCTTCAGG TGGGCAATGA AGCCCCTGTG 
ACTGGCTCGT CTCCACTGGG CTCCACACAG CTGGACACAG ATGGAGCCCT GTGGCTCGGG 
GGCCTTCAGA AGCTGCCTGT GGGCCAGGCC CTGCCCAAGG CCTACAGTAC AGGCTTCGTG 
GGCTGCTTAC GGGATGTAGT GGTGAGCGGG CGCCCACTGC ACCTGCTGGA AGATGCAGTC 
ACCAGGCCAG AGCTGCGGCC CTGCCCTGCA CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008830.1
CDS	209..5884
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESKLANEIPFPEAPDSGSFHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153376.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153376.1

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGA CCAGTGTGGA 
CAGTGTCGTT TTGGAGCCCT GTGTGAGGCC GAAACCGGGC GCTGTGTGTG CCCCTCTGAG 
TGTGTGGCCT CAGCCCAGCC TGTGTGTGGT TCTGATGGGC ACACATATGC CAGTGAATGT 
GAGCTTCATG TCCACGCCTG TACACAGCAG GTTGATCTGC ACGTGATCTC AGCTGGACCC 
TGCCAGACCT GTAAGGATAC AGTGTGTGCC TTTGGGGCAG TGTGCTCAGC TGGGCAGTGC 
GTGTGTCCTC GGTGTGAGCA CCCCCCACCA GGCCCCGTGT GCGGCAGTGA TGGTATCACC 
TACCCCAGTG CATGTGAGCT GCGGGAAGCT GCCTGCCGGC AGCAGGTACA AATTGAAGAA 
GCCCGGGCAG GACCCTGTGA GCAAGCTGAA TGTGGCTCAG GAGGCTCTGG CTCTGGAGAG 
GAAGGCGAGT GTGAGCGGGA ACTGTGCCAG CAGCGTGGTG GCATCTGGGA TGAGGACTCA 
GAGGATGGGC CATGTGTCTG TGACTTCAGC TGTCAGAGTG TCCTGAGGAG CCCGGTATGC 
GGCTCAGATG GGGTTACCTA TGGCACTGAG TGTGACCTGA AGAAGGCCAG GTGTGAATCA 
CAACGAGAAC TGTACGTCGC GGCTCAGGGG CCCTGCCACA GTCCTACATT GGCACCACTG 
CCACCTGTGT TTCTTTCACA CTGTGCCCAG ACCCCATATG GCTGCTGCCA GGACAATGTC 
ACTGCTGCCA GGGGTGTGGG CCTGGCTGGC TGCCCCAGCA CCTGCCAGTG CAACCCACAT 
GGCTCCTACA GTGGCACCTG TGACCCAGCC ACAGGGCAGT GCTCCTGCCG ACCAGGCGTG 
GGAGGCCTTA AGTGTGACCG CTGTGAGCCT GGCTTCTGGA ATTTCCGTGG CATTGTCACT 
GACAACCTGA GTGGCTGTAC TCCCTGCAGC TGTGACCCCC AGGGTGCTGT GCGGGACGAC 
TGTGAGCAGA TGACTGGACT GTGCTCCTGC AAGCCAGGAG TGGCTGGGCC CAAGTGTGGG 
CAGTGTCCAG ATGGCCAAAC CCTGGGCCCT ACTGGTTGTG AAGCAGGCTC CTTGATGCCC 
AAGACCTGTG TGGAGATGCA CTGTGAGTTT GGAGCTTCCT GTGTGGAAGA AGCTGGTTCT 
GCCCGCTGTG CCTGCCCAGC CCTCACGTGC CCAGAGGCCA ATGCCACTAA GGTCTGTGGG 
TCAGATGGTA TCACATACGG CAATGAGTGC CAGCTGAAGA CGATTGCCTG CCGCCAGGGC 
CTGGACATCT TCACCCAGAG TGTTGGCCCA TGCCAGGATG CTGTTGCTCT TGGCGTATAC 
CCCACATCTG TATCCATGAC CACCCTGGAG CATGTCCTGA ATGAGGCGCT GCCGTCCCCC 
TCCAGTGCCC TTTCCCTGGC ACCCAGCAGT ATTCCCCGCA GCCAGGCTAC TCCTCTACCC 
ACATCACGAC CCTGGACCAC ATCCAGCATC CCCAGGCCCA CGGCAAGACC TGGGCCTGTG 
CTAACCATGC CCCCCACAAC CACCTCCACC ACTGCCAGCC TTCCCGCATC AGCCTTCGGT 
GAATCTGGCA GCACCAATGG GAGTGGTGAT GAGGAGCTCA GCGGAGACCA GGAGGCCAGT 
GGGGGAGGCT CTGGGGGACT TGAGCTCCCC ATGGGCAACA ACATTGTGAC TGCTGGGCCA 
CCCATCGAGA GGGCTTCCTG CTACAACTCT CCTTTGGGCT GCTGTTCAGA TGGCAAGACA 
CCCTCACTGG ATGCAGAGAG CTCCAACTGC CCTGCCACCA AGGCGTTCCA GGGCGTACTG 
GAGCTGGAGG GGGTCGAGGG GCAGGAGCTG TTCTACACAC CTGAGATGGC TGACCCCAAG 
TCAGAACTGT TTGGGGAAAC TGCCAGGAGC ATTGAGAACT CGCTGGATGA CCTGTTCCGA 
AACTCAGATG TCAAGAAGGA CTTCCGGAGT GTCCGCCTGC GGGACCTGGG GCCTGGCAGA 
TCGGTCCGAG CTATTGTGGA TGTGCACTTT GACCCCACTA CGGCCTTCAG GGCTCCCGAT 
GTGGGCCAGG CCCTCCTCCG GCAGATCCAG GCATCCAGAC GCCGGACCTT ACTGGTGAGG 
AGGCCTCTTC ATGAACATGT GCGATTCATG GACTTTGACT GGTTTCCAGC CTTCTTCACA 
GGAGCTGCTA CAGGAGCCAC GGCTGCAGTG GCCACAGCCA GAGCCACCAC CACAGGCCAG 
CTGCCCTCTG CTGTAACCCC TCGGGCCTAC CCCAGTCACA CCAGCCGACC TACTGGCAAG 
ACCACAGCAC CCCTCACCAC ACGCCGGCCT CTCACTACGC TCCCCAGCCG CATGGCAGGA 
CACCAACCCA TTCCCCCAGA CCCTCAGCAG CCACTGAGGC CCTGTGCCTC CCAGCCCTGC 
CTCTATGGGG GCACGTGCTG GGACCAGGAC TCTGGCCAAG GCTTCACTTG CAGCTGCCCA 
GCGGGCAGGG GCGGCATCAT CTGCGAGGAG GTATTGCGCC CCTCTCTGCC TGCTTTTGGA 
GGCCACTCCT TCCTGGCCTT CCCCACCCTA CGCGCCTACC ACACACTGCG CCTGGCACTA 
GAATTCCGAA CACTGGAGCT GCAGGGGCTG CTGCTCTACA ATGGGAACAA CCGTGGCAAA 
GACTTCTTGG CGCTGGCTCT ACAGGGTGGG CGGGTGCAGC TCAGGTTTGA CACAGGTTCT 
GGGCCAGCAG TGTTGACCAG TTCCGTGCCT GTGAAACTGG GCCGGTGGCA CCGCCTAGAA 
CTGTCAAGGC ATTGGCGCCA GGGCACACTG TCTGTGGATG GCGAGAGCCC TGTTGTGGGC 
GAGAGCCCCA GTGGCACGGA TGGCCTTAAC CTGGACACGG ACCTTTTTGT GGGTGGCGTC 
CCAGAGGACC AGGCTGCTGT GGTGCTTGAG CGGACCTTCG TTGCAGTTGG CCTGAAAGGC 
TGCATCCGGT TGCTGGACAT CAACAACCAG CGGCTGGAGC TGGGCAGCTG GCAAGGAGCT 
TCGACCCAGA GCTCTGGCGT GGGCGAGTGT GGGGAAAACC CCTGTGTGCC CAACCCCTGC 
AGCAGTGGGG CGCCATGCCA GGCACTTGAC GATGGCATGT TCCACTGTCA GTGCCCACCT 
GGCCGCTTTG GTCTGACCTG TGCCAGTGAG AAGAACCCCT GCCAGCCAAA CCCTTGCCAT 
GGAGCAGCCC CTTGCCGTGT ACTACCCGAG GGAGGGCCGA AGTGCGAGTG CCCCCTTGGG 
CGTGGGGGCG CCCTCTGCCA GACAGACTTG GAGAAGGACC ATGGCTCCCA GCCCTTCATG 
GCGGATTTCA GCGGCTTCTC CTATCTGGAG CTGAAAGGCC TGTACACCTT TGAAAGAGAC 
CTGGGAGAAA AGATGGCGCT GGAGGTTGTG TTCCTGGCCC GTGGCCCCAG TGGCTTGCTC 
CTTTACAATG GGCAGAAGAC AGATGGCAGA GGAGACTTTG TGTCGCTGGC CCTGCGTGAC 
TGGCATCTGG AATTCCGTTA TGACCTGGGC AAGGGCCCTG CAGTCATCAG GAGCAAGGAG 
CCAGTCGCCG TGGACACCTG GACAAGGGTC TCACTGGAGC GCAATGGCCG CAAGGGTGCC 
ATGCGTGTGG GCGATGGGCC CCGAGTGCTG GGGGAGTCCC CGAAATCCCG CAAGGTGCCT 
CACACTGTGC TCAACCTGAA GGAGCCACTC TATGTGGGGG GCGCCCCTGA TTTCAGTAGG 
TTGGCCCGGG CAGCTGCGGT GTCCTCTGGC TTTGATGGTG CCATCCAGCT GGTTTCCCTG 
GGAGGCCGCC AGTTGCTGAC CCAGGAACAT GTGCTGCAGG CAGTGGGCAT CTCCTCCTTT 
GCAGACCACC CTTGCACCCA AGCCTCGGGC CACCCCTGTC TTAATGGAGC CTCGTGTCTC 
CCCCAGGAAG CCACCTATGA GTGCCTGTGT CCCTCAGGCT TCTCAGGGCT GCACTGTGAG 
AAGGGACTGG TTGAAAAGTC GGCGGGGGAC CTGGACACAT TGGCCTTTGA TGGGCAGACC 
TACATCGAGT ACCTCAATGC TGTGAGGGAG AGCAAACTGG CCAATGAGAT CCCATTCCCG 
GAAGCTCCCG ATTCCGGGTC CTTTCACAGC GAGAAGGCGC TGCAGAGCAA CCACTTCGAG 
CTGAGCCTGC GCACCGAGGC CACTCAGGGG CTGGTGCTGT GGAGCGGCAA GGCCACAGAG 
CGTGCAGACT ATGTGGCTCT GGCCATCGTG GATGGGTACC TGCAGCTGAG CTACGACCTG 
GGCTCCCAGC CCGTGGTGCT GCGCTCCACT GTGAAGGTCA ACACCAATCA CTGGCTGCGG 
GTTAAGGCTC ACAGGGAGCA GAGGGAAGGG TCCCTTCAGG TGGGCAATGA AGCCCCTGTG 
ACTGGCTCGT CTCCACTGGG CTCCACACAG CTGGACACAG ATGGAGCCCT GTGGCTCGGG 
GGCCTTCAGA AGCTGCCTGT GGGCCAGGCC CTGCCCAAGG CCTACAGTAC AGGCTTCGTG 
GGCTGCTTAC GGGATGTAGT GGTGAGCGGG CGCCCACTGC ACCTGCTGGA AGATGCAGTC 
ACCAGGCCAG AGCTGCGGCC CTGCCCTGCA CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_013153379.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_013008833.1 Cavia porcellus agrin (Agrn), transcript variant X9, mRNA.

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CloneID	ODo28105
Clone ID Related Accession (Same CDS sequence)	XM_013153379.1
Accession Version	XM_013153379.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCGAGC TGGGGGCCAT GGCCTGTGCC CTGGGGCAGG AGATCCGGGT GACCCGCCGC 
GGACCCTGTG ACCAGTGTGG ACAGTGTCGT TTTGGAGCCC TGTGTGAGGC CGAAACCGGG 
CGCTGTGTGT GCCCCTCTGA GTGTGTGGCC TCAGCCCAGC CTGTGTGTGG TTCTGATGGG 
CACACATATG CCAGTGAATG TGAGCTTCAT GTCCACGCCT GTACACAGCA GGTTGATCTG 
CACGTGATCT CAGCTGGACC CTGCCAGACC TGTAAGGATA CAGTGTGTGC CTTTGGGGCA 
GTGTGCTCAG CTGGGCAGTG CGTGTGTCCT CGGTGTGAGC ACCCCCCACC AGGCCCCGTG 
TGCGGCAGTG ATGGTATCAC CTACCCCAGT GCATGTGAGC TGCGGGAAGC TGCCTGCCGG 
CAGCAGGTAC AAATTGAAGA AGCCCGGGCA GGACCCTGTG AGCAAGCTGA ATGTGGCTCA 
GGAGGCTCTG GCTCTGGAGA GGAAGGCGAG TGTGAGCGGG AACTGTGCCA GCAGCGTGGT 
GGCATCTGGG ATGAGGACTC AGAGGATGGG CCATGTGTCT GTGACTTCAG CTGTCAGAGT 
GTCCTGAGGA GCCCGGTATG CGGCTCAGAT GGGGTTACCT ATGGCACTGA GTGTGACCTG 
AAGAAGGCCA GGTGTGAATC ACAACGAGAA CTGTACGTCG CGGCTCAGGG GCCCTGCCAC 
AGTCCTACAT TGGCACCACT GCCACCTGTG TTTCTTTCAC ACTGTGCCCA GACCCCATAT 
GGCTGCTGCC AGGACAATGT CACTGCTGCC AGGGGTGTGG GCCTGGCTGG CTGCCCCAGC 
ACCTGCCAGT GCAACCCACA TGGCTCCTAC AGTGGCACCT GTGACCCAGC CACAGGGCAG 
TGCTCCTGCC GACCAGGCGT GGGAGGCCTT AAGTGTGACC GCTGTGAGCC TGGCTTCTGG 
AATTTCCGTG GCATTGTCAC TGACAACCTG AGTGGCTGTA CTCCCTGCAG CTGTGACCCC 
CAGGGTGCTG TGCGGGACGA CTGTGAGCAG ATGACTGGAC TGTGCTCCTG CAAGCCAGGA 
GTGGCTGGGC CCAAGTGTGG GCAGTGTCCA GATGGCCAAA CCCTGGGCCC TACTGGTTGT 
GAAGCAGGCT CCTTGATGCC CAAGACCTGT GTGGAGATGC ACTGTGAGTT TGGAGCTTCC 
TGTGTGGAAG AAGCTGGTTC TGCCCGCTGT GCCTGCCCAG CCCTCACGTG CCCAGAGGCC 
AATGCCACTA AGGTCTGTGG GTCAGATGGT ATCACATACG GCAATGAGTG CCAGCTGAAG 
ACGATTGCCT GCCGCCAGGG CCTGGACATC TTCACCCAGA GTGTTGGCCC ATGCCAGGAT 
GCTGTTGCTC TTGGCGTATA CCCCACATCT GTATCCATGA CCACCCTGGA GCATGTCCTG 
AATGAGGCGC TGCCGTCCCC CTCCAGTGCC CTTTCCCTGG CACCCAGCAG TATTCCCCGC 
AGCCAGGCTA CTCCTCTACC CACATCACGA CCCTGGACCA CATCCAGCAT CCCCAGGCCC 
ACGGCAAGAC CTGGGCCTGT GCTAACCATG CCCCCCACAA CCACCTCCAC CACTGCCAGC 
CTTCCCGCAT CAGCCTTCGG TGAATCTGGC AGCACCAATG GGAGTGGTGA TGAGGAGCTC 
AGCGGAGACC AGGAGGCCAG TGGGGGAGGC TCTGGGGGAC TTGAGCTCCC CATGGGCAAC 
AACATTGTGA CTGCTGGGCC ACCCATCGAG AGGGCTTCCT GCTACAACTC TCCTTTGGGC 
TGCTGTTCAG ATGGCAAGAC ACCCTCACTG GATGCAGAGA GCTCCAACTG CCCTGCCACC 
AAGGCGTTCC AGGGCGTACT GGAGCTGGAG GGGGTCGAGG GGCAGGAGCT GTTCTACACA 
CCTGAGATGG CTGACCCCAA GTCAGAACTG TTTGGGGAAA CTGCCAGGAG CATTGAGAAC 
TCGCTGGATG ACCTGTTCCG AAACTCAGAT GTCAAGAAGG ACTTCCGGAG TGTCCGCCTG 
CGGGACCTGG GGCCTGGCAG ATCGGTCCGA GCTATTGTGG ATGTGCACTT TGACCCCACT 
ACGGCCTTCA GGGCTCCCGA TGTGGGCCAG GCCCTCCTCC GGCAGATCCA GGCATCCAGA 
CGCCGGACCT TACTGGTGAG GAGGCCTCTT CATGAACATG TGCGATTCAT GGACTTTGAC 
TGGTTTCCAG CCTTCTTCAC AGGAGCTGCT ACAGGAGCCA CGGCTGCAGT GGCCACAGCC 
AGAGCCACCA CCACAGGCCA GCTGCCCTCT GCTGTAACCC CTCGGGCCTA CCCCAGTCAC 
ACCAGCCGAC CTACTGGCAA GACCACAGCA CCCCTCACCA CACGCCGGCC TCTCACTACG 
CTCCCCAGCC GCATGGCAGG ACACCAACCC ATTCCCCCAG ACCCTCAGCA GCCACTGAGG 
CCCTGTGCCT CCCAGCCCTG CCTCTATGGG GGCACGTGCT GGGACCAGGA CTCTGGCCAA 
GGCTTCACTT GCAGCTGCCC AGCGGGCAGG GGCGGCATCA TCTGCGAGGA GGTATTGCGC 
CCCTCTCTGC CTGCTTTTGG AGGCCACTCC TTCCTGGCCT TCCCCACCCT ACGCGCCTAC 
CACACACTGC GCCTGGCACT AGAATTCCGA ACACTGGAGC TGCAGGGGCT GCTGCTCTAC 
AATGGGAACA ACCGTGGCAA AGACTTCTTG GCGCTGGCTC TACAGGGTGG GCGGGTGCAG 
CTCAGGTTTG ACACAGGTTC TGGGCCAGCA GTGTTGACCA GTTCCGTGCC TGTGAAACTG 
GGCCGGTGGC ACCGCCTAGA ACTGTCAAGG CATTGGCGCC AGGGCACACT GTCTGTGGAT 
GGCGAGAGCC CTGTTGTGGG CGAGAGCCCC AGTGGCACGG ATGGCCTTAA CCTGGACACG 
GACCTTTTTG TGGGTGGCGT CCCAGAGGAC CAGGCTGCTG TGGTGCTTGA GCGGACCTTC 
GTTGCAGTTG GCCTGAAAGG CTGCATCCGG TTGCTGGACA TCAACAACCA GCGGCTGGAG 
CTGGGCAGCT GGCAAGGAGC TTCGACCCAG AGCTCTGGCG TGGGCGAGTG TGGGGAAAAC 
CCCTGTGTGC CCAACCCCTG CAGCAGTGGG GCGCCATGCC AGGCACTTGA CGATGGCATG 
TTCCACTGTC AGTGCCCACC TGGCCGCTTT GGTCTGACCT GTGCCAGTGA GAAGAACCCC 
TGCCAGCCAA ACCCTTGCCA TGGAGCAGCC CCTTGCCGTG TACTACCCGA GGGAGGGCCG 
AAGTGCGAGT GCCCCCTTGG GCGTGGGGGC GCCCTCTGCC AGACAGACTT GGAGAAGGAC 
CATGGCTCCC AGCCCTTCAT GGCGGATTTC AGCGGCTTCT CCTATCTGGA GCTGAAAGGC 
CTGTACACCT TTGAAAGAGA CCTGGGAGAA AAGATGGCGC TGGAGGTTGT GTTCCTGGCC 
CGTGGCCCCA GTGGCTTGCT CCTTTACAAT GGGCAGAAGA CAGATGGCAG AGGAGACTTT 
GTGTCGCTGG CCCTGCGTGA CTGGCATCTG GAATTCCGTT ATGACCTGGG CAAGGGCCCT 
GCAGTCATCA GGAGCAAGGA GCCAGTCGCC GTGGACACCT GGACAAGGGT CTCACTGGAG 
CGCAATGGCC GCAAGGGTGC CATGCGTGTG GGCGATGGGC CCCGAGTGCT GGGGGAGTCC 
CCGAAATCCC GCAAGGTGCC TCACACTGTG CTCAACCTGA AGGAGCCACT CTATGTGGGG 
GGCGCCCCTG ATTTCAGTAG GTTGGCCCGG GCAGCTGCGG TGTCCTCTGG CTTTGATGGT 
GCCATCCAGC TGGTTTCCCT GGGAGGCCGC CAGTTGCTGA CCCAGGAACA TGTGCTGCAG 
GCAGTGGGCA TCTCCTCCTT TGCAGACCAC CCTTGCACCC AAGCCTCGGG CCACCCCTGT 
CTTAATGGAG CCTCGTGTCT CCCCCAGGAA GCCACCTATG AGTGCCTGTG TCCCTCAGGC 
TTCTCAGGGC TGCACTGTGA GAAGGGACTG GTTGAAAAGT CGGCGGGGGA CCTGGACACA 
TTGGCCTTTG ATGGGCAGAC CTACATCGAG TACCTCAATG CTGTGAGGGA GAGCAAACTG 
GCCAATGAGA TCCCATTCCC GGAAGCTCCC GATTCCGGGT CCTTTCACAG CGAGAAGGCG 
CTGCAGAGCA ACCACTTCGA GCTGAGCCTG CGCACCGAGG CCACTCAGGG GCTGGTGCTG 
TGGAGCGGCA AGGCCACAGA GCGTGCAGAC TATGTGGCTC TGGCCATCGT GGATGGGTAC 
CTGCAGCTGA GCTACGACCT GGGCTCCCAG CCCGTGGTGC TGCGCTCCAC TGTGAAGGTC 
AACACCAATC ACTGGCTGCG GGTTAAGGCT CACAGGGAGC AGAGGGAAGG GTCCCTTCAG 
GTGGGCAATG AAGCCCCTGT GACTGGCTCG TCTCCACTGG GCTCCACACA GCTGGACACA 
GATGGAGCCC TGTGGCTCGG GGGCCTTCAG AAGCTGCCTG TGGGCCAGGC CCTGCCCAAG 
GCCTACAGTA CAGGCTTCGT GGGCTGCTTA CGGGATGTAG TGGTGAGCGG GCGCCCACTG 
CACCTGCTGG AAGATGCAGT CACCAGGCCA GAGCTGCGGC CCTGCCCTGC ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013008833.1
CDS	740..5416
Translation	MCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESKLANEIPFPEAPDSGSFHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_013153379.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013153379.1

ORF Insert Sequence:

ATGTGCGAGC TGGGGGCCAT GGCCTGTGCC CTGGGGCAGG AGATCCGGGT GACCCGCCGC 
GGACCCTGTG ACCAGTGTGG ACAGTGTCGT TTTGGAGCCC TGTGTGAGGC CGAAACCGGG 
CGCTGTGTGT GCCCCTCTGA GTGTGTGGCC TCAGCCCAGC CTGTGTGTGG TTCTGATGGG 
CACACATATG CCAGTGAATG TGAGCTTCAT GTCCACGCCT GTACACAGCA GGTTGATCTG 
CACGTGATCT CAGCTGGACC CTGCCAGACC TGTAAGGATA CAGTGTGTGC CTTTGGGGCA 
GTGTGCTCAG CTGGGCAGTG CGTGTGTCCT CGGTGTGAGC ACCCCCCACC AGGCCCCGTG 
TGCGGCAGTG ATGGTATCAC CTACCCCAGT GCATGTGAGC TGCGGGAAGC TGCCTGCCGG 
CAGCAGGTAC AAATTGAAGA AGCCCGGGCA GGACCCTGTG AGCAAGCTGA ATGTGGCTCA 
GGAGGCTCTG GCTCTGGAGA GGAAGGCGAG TGTGAGCGGG AACTGTGCCA GCAGCGTGGT 
GGCATCTGGG ATGAGGACTC AGAGGATGGG CCATGTGTCT GTGACTTCAG CTGTCAGAGT 
GTCCTGAGGA GCCCGGTATG CGGCTCAGAT GGGGTTACCT ATGGCACTGA GTGTGACCTG 
AAGAAGGCCA GGTGTGAATC ACAACGAGAA CTGTACGTCG CGGCTCAGGG GCCCTGCCAC 
AGTCCTACAT TGGCACCACT GCCACCTGTG TTTCTTTCAC ACTGTGCCCA GACCCCATAT 
GGCTGCTGCC AGGACAATGT CACTGCTGCC AGGGGTGTGG GCCTGGCTGG CTGCCCCAGC 
ACCTGCCAGT GCAACCCACA TGGCTCCTAC AGTGGCACCT GTGACCCAGC CACAGGGCAG 
TGCTCCTGCC GACCAGGCGT GGGAGGCCTT AAGTGTGACC GCTGTGAGCC TGGCTTCTGG 
AATTTCCGTG GCATTGTCAC TGACAACCTG AGTGGCTGTA CTCCCTGCAG CTGTGACCCC 
CAGGGTGCTG TGCGGGACGA CTGTGAGCAG ATGACTGGAC TGTGCTCCTG CAAGCCAGGA 
GTGGCTGGGC CCAAGTGTGG GCAGTGTCCA GATGGCCAAA CCCTGGGCCC TACTGGTTGT 
GAAGCAGGCT CCTTGATGCC CAAGACCTGT GTGGAGATGC ACTGTGAGTT TGGAGCTTCC 
TGTGTGGAAG AAGCTGGTTC TGCCCGCTGT GCCTGCCCAG CCCTCACGTG CCCAGAGGCC 
AATGCCACTA AGGTCTGTGG GTCAGATGGT ATCACATACG GCAATGAGTG CCAGCTGAAG 
ACGATTGCCT GCCGCCAGGG CCTGGACATC TTCACCCAGA GTGTTGGCCC ATGCCAGGAT 
GCTGTTGCTC TTGGCGTATA CCCCACATCT GTATCCATGA CCACCCTGGA GCATGTCCTG 
AATGAGGCGC TGCCGTCCCC CTCCAGTGCC CTTTCCCTGG CACCCAGCAG TATTCCCCGC 
AGCCAGGCTA CTCCTCTACC CACATCACGA CCCTGGACCA CATCCAGCAT CCCCAGGCCC 
ACGGCAAGAC CTGGGCCTGT GCTAACCATG CCCCCCACAA CCACCTCCAC CACTGCCAGC 
CTTCCCGCAT CAGCCTTCGG TGAATCTGGC AGCACCAATG GGAGTGGTGA TGAGGAGCTC 
AGCGGAGACC AGGAGGCCAG TGGGGGAGGC TCTGGGGGAC TTGAGCTCCC CATGGGCAAC 
AACATTGTGA CTGCTGGGCC ACCCATCGAG AGGGCTTCCT GCTACAACTC TCCTTTGGGC 
TGCTGTTCAG ATGGCAAGAC ACCCTCACTG GATGCAGAGA GCTCCAACTG CCCTGCCACC 
AAGGCGTTCC AGGGCGTACT GGAGCTGGAG GGGGTCGAGG GGCAGGAGCT GTTCTACACA 
CCTGAGATGG CTGACCCCAA GTCAGAACTG TTTGGGGAAA CTGCCAGGAG CATTGAGAAC 
TCGCTGGATG ACCTGTTCCG AAACTCAGAT GTCAAGAAGG ACTTCCGGAG TGTCCGCCTG 
CGGGACCTGG GGCCTGGCAG ATCGGTCCGA GCTATTGTGG ATGTGCACTT TGACCCCACT 
ACGGCCTTCA GGGCTCCCGA TGTGGGCCAG GCCCTCCTCC GGCAGATCCA GGCATCCAGA 
CGCCGGACCT TACTGGTGAG GAGGCCTCTT CATGAACATG TGCGATTCAT GGACTTTGAC 
TGGTTTCCAG CCTTCTTCAC AGGAGCTGCT ACAGGAGCCA CGGCTGCAGT GGCCACAGCC 
AGAGCCACCA CCACAGGCCA GCTGCCCTCT GCTGTAACCC CTCGGGCCTA CCCCAGTCAC 
ACCAGCCGAC CTACTGGCAA GACCACAGCA CCCCTCACCA CACGCCGGCC TCTCACTACG 
CTCCCCAGCC GCATGGCAGG ACACCAACCC ATTCCCCCAG ACCCTCAGCA GCCACTGAGG 
CCCTGTGCCT CCCAGCCCTG CCTCTATGGG GGCACGTGCT GGGACCAGGA CTCTGGCCAA 
GGCTTCACTT GCAGCTGCCC AGCGGGCAGG GGCGGCATCA TCTGCGAGGA GGTATTGCGC 
CCCTCTCTGC CTGCTTTTGG AGGCCACTCC TTCCTGGCCT TCCCCACCCT ACGCGCCTAC 
CACACACTGC GCCTGGCACT AGAATTCCGA ACACTGGAGC TGCAGGGGCT GCTGCTCTAC 
AATGGGAACA ACCGTGGCAA AGACTTCTTG GCGCTGGCTC TACAGGGTGG GCGGGTGCAG 
CTCAGGTTTG ACACAGGTTC TGGGCCAGCA GTGTTGACCA GTTCCGTGCC TGTGAAACTG 
GGCCGGTGGC ACCGCCTAGA ACTGTCAAGG CATTGGCGCC AGGGCACACT GTCTGTGGAT 
GGCGAGAGCC CTGTTGTGGG CGAGAGCCCC AGTGGCACGG ATGGCCTTAA CCTGGACACG 
GACCTTTTTG TGGGTGGCGT CCCAGAGGAC CAGGCTGCTG TGGTGCTTGA GCGGACCTTC 
GTTGCAGTTG GCCTGAAAGG CTGCATCCGG TTGCTGGACA TCAACAACCA GCGGCTGGAG 
CTGGGCAGCT GGCAAGGAGC TTCGACCCAG AGCTCTGGCG TGGGCGAGTG TGGGGAAAAC 
CCCTGTGTGC CCAACCCCTG CAGCAGTGGG GCGCCATGCC AGGCACTTGA CGATGGCATG 
TTCCACTGTC AGTGCCCACC TGGCCGCTTT GGTCTGACCT GTGCCAGTGA GAAGAACCCC 
TGCCAGCCAA ACCCTTGCCA TGGAGCAGCC CCTTGCCGTG TACTACCCGA GGGAGGGCCG 
AAGTGCGAGT GCCCCCTTGG GCGTGGGGGC GCCCTCTGCC AGACAGACTT GGAGAAGGAC 
CATGGCTCCC AGCCCTTCAT GGCGGATTTC AGCGGCTTCT CCTATCTGGA GCTGAAAGGC 
CTGTACACCT TTGAAAGAGA CCTGGGAGAA AAGATGGCGC TGGAGGTTGT GTTCCTGGCC 
CGTGGCCCCA GTGGCTTGCT CCTTTACAAT GGGCAGAAGA CAGATGGCAG AGGAGACTTT 
GTGTCGCTGG CCCTGCGTGA CTGGCATCTG GAATTCCGTT ATGACCTGGG CAAGGGCCCT 
GCAGTCATCA GGAGCAAGGA GCCAGTCGCC GTGGACACCT GGACAAGGGT CTCACTGGAG 
CGCAATGGCC GCAAGGGTGC CATGCGTGTG GGCGATGGGC CCCGAGTGCT GGGGGAGTCC 
CCGAAATCCC GCAAGGTGCC TCACACTGTG CTCAACCTGA AGGAGCCACT CTATGTGGGG 
GGCGCCCCTG ATTTCAGTAG GTTGGCCCGG GCAGCTGCGG TGTCCTCTGG CTTTGATGGT 
GCCATCCAGC TGGTTTCCCT GGGAGGCCGC CAGTTGCTGA CCCAGGAACA TGTGCTGCAG 
GCAGTGGGCA TCTCCTCCTT TGCAGACCAC CCTTGCACCC AAGCCTCGGG CCACCCCTGT 
CTTAATGGAG CCTCGTGTCT CCCCCAGGAA GCCACCTATG AGTGCCTGTG TCCCTCAGGC 
TTCTCAGGGC TGCACTGTGA GAAGGGACTG GTTGAAAAGT CGGCGGGGGA CCTGGACACA 
TTGGCCTTTG ATGGGCAGAC CTACATCGAG TACCTCAATG CTGTGAGGGA GAGCAAACTG 
GCCAATGAGA TCCCATTCCC GGAAGCTCCC GATTCCGGGT CCTTTCACAG CGAGAAGGCG 
CTGCAGAGCA ACCACTTCGA GCTGAGCCTG CGCACCGAGG CCACTCAGGG GCTGGTGCTG 
TGGAGCGGCA AGGCCACAGA GCGTGCAGAC TATGTGGCTC TGGCCATCGT GGATGGGTAC 
CTGCAGCTGA GCTACGACCT GGGCTCCCAG CCCGTGGTGC TGCGCTCCAC TGTGAAGGTC 
AACACCAATC ACTGGCTGCG GGTTAAGGCT CACAGGGAGC AGAGGGAAGG GTCCCTTCAG 
GTGGGCAATG AAGCCCCTGT GACTGGCTCG TCTCCACTGG GCTCCACACA GCTGGACACA 
GATGGAGCCC TGTGGCTCGG GGGCCTTCAG AAGCTGCCTG TGGGCCAGGC CCTGCCCAAG 
GCCTACAGTA CAGGCTTCGT GGGCTGCTTA CGGGATGTAG TGGTGAGCGG GCGCCCACTG 
CACCTGCTGG AAGATGCAGT CACCAGGCCA GAGCTGCGGC CCTGCCCTGC ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_004999693.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_004999750.2 Cavia porcellus agrin (Agrn), transcript variant X8, mRNA.

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CloneID	ODo28106
Clone ID Related Accession (Same CDS sequence)	XM_004999693.2
Accession Version	XM_004999693.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 14, 2015 this sequence version replaced XM_004999693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCTC TCCCGCTGGA GCGCCGTCCC GGGCTGCAGC CCGGTGTCTC CGTGCTGGTG 
CGGTACTTCA TGGTCCCCTG CAATGCTTGC CTCATCCTGC TGGCCACCTC CACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAGCAATTAC AAACTTGGGA CACACTTCAC GCCAGCCCCT 
CTGATGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCAAACTGGC CAATGAGATC CCATTCCCGG AAGCTCCCGA TTCCGGGTCC 
TTTCACAGCG AGAAGGCGCT GCAGAGCAAC CACTTCGAGC TGAGCCTGCG CACCGAGGCC 
ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACAGAGC GTGCAGACTA TGTGGCTCTG 
GCCATCGTGG ATGGGTACCT GCAGCTGAGC TACGACCTGG GCTCCCAGCC CGTGGTGCTG 
CGCTCCACTG TGAAGGTCAA CACCAATCAC TGGCTGCGGG TTAAGGCTCA CAGGGAGCAG 
AGGGAAGGGT CCCTTCAGGT GGGCAATGAA GCCCCTGTGA CTGGCTCGTC TCCACTGGGC 
TCCACACAGC TGGACACAGA TGGAGCCCTG TGGCTCGGGG GCCTTCAGAA GCTGCCTGTG 
GGCCAGGCCC TGCCCAAGGC CTACAGTACA GGCTTCGTGG GCTGCTTACG GGATGTAGTG 
GTGAGCGGGC GCCCACTGCA CCTGCTGGAA GATGCAGTCA CCAGGCCAGA GCTGCGGCCC 
TGCCCTGCAC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004999750.2
CDS	349..6243
Translation	MPPLPLERRPGLQPGVSVLVRYFMVPCNACLILLATSTLGFAVLLFLSNYKLGTHFTPAPLMPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESKLANEIPFPEAPDSGSFHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_004999693.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004999693.2

ORF Insert Sequence:

ATGCCGCCTC TCCCGCTGGA GCGCCGTCCC GGGCTGCAGC CCGGTGTCTC CGTGCTGGTG 
CGGTACTTCA TGGTCCCCTG CAATGCTTGC CTCATCCTGC TGGCCACCTC CACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAGCAATTAC AAACTTGGGA CACACTTCAC GCCAGCCCCT 
CTGATGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCAAACTGGC CAATGAGATC CCATTCCCGG AAGCTCCCGA TTCCGGGTCC 
TTTCACAGCG AGAAGGCGCT GCAGAGCAAC CACTTCGAGC TGAGCCTGCG CACCGAGGCC 
ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACAGAGC GTGCAGACTA TGTGGCTCTG 
GCCATCGTGG ATGGGTACCT GCAGCTGAGC TACGACCTGG GCTCCCAGCC CGTGGTGCTG 
CGCTCCACTG TGAAGGTCAA CACCAATCAC TGGCTGCGGG TTAAGGCTCA CAGGGAGCAG 
AGGGAAGGGT CCCTTCAGGT GGGCAATGAA GCCCCTGTGA CTGGCTCGTC TCCACTGGGC 
TCCACACAGC TGGACACAGA TGGAGCCCTG TGGCTCGGGG GCCTTCAGAA GCTGCCTGTG 
GGCCAGGCCC TGCCCAAGGC CTACAGTACA GGCTTCGTGG GCTGCTTACG GGATGTAGTG 
GTGAGCGGGC GCCCACTGCA CCTGCTGGAA GATGCAGTCA CCAGGCCAGA GCTGCGGCCC 
TGCCCTGCAC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_023565389.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_023421157.1 Cavia porcellus agrin (Agrn), transcript variant X1, mRNA.

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CloneID	ODo41202
Clone ID Related Accession (Same CDS sequence)	XM_023565389.1
Accession Version	XM_023565389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCAAACTGGC CAATGAGATC CCATTCCCGG AAGCTCCCGA TTCCGGGTCC 
TTTCACAGCG AGAAGGCGCT GCAGAGCAAC CACTTCGAGC TGAGCCTGCG CACCGAGGCC 
ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACAGAGC GTGCAGACTA TGTGGCTCTG 
GCCATCGTGG ATGGGTACCT GCAGCTGAGC TACGACCTGG GCTCCCAGCC CGTGGTGCTG 
CGCTCCACTG TGAAGGTCAA CACCAATCAC TGGCTGCGGG TTAAGGCTCA CAGGGAGCAG 
AGGGAAGGGT CCCTTCAGGT GGGCAATGAA GCCCCTGTGA CTGGCTCGTC TCCACTGGGC 
TCCACACAGC TGGACACAGA TGGAGCCCTG TGGCTCGGGG GCCTTCAGAA GCTGCCTGTG 
GGCCAGGCCC TGCCCAAGGC CTACAGTACA GGCTTCGTGG GCTGCTTACG GGATGTAGTG 
GTGAGCGGGC GCCCACTGCA CCTGCTGGAA GATGCAGTCA CCAGGCCAGA GCTGCGGCCC 
TGCCCTGCAC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023421157.1
CDS	188..6082
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESKLANEIPFPEAPDSGSFHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_023565389.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023565389.1

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCAAACTGGC CAATGAGATC CCATTCCCGG AAGCTCCCGA TTCCGGGTCC 
TTTCACAGCG AGAAGGCGCT GCAGAGCAAC CACTTCGAGC TGAGCCTGCG CACCGAGGCC 
ACTCAGGGGC TGGTGCTGTG GAGCGGCAAG GCCACAGAGC GTGCAGACTA TGTGGCTCTG 
GCCATCGTGG ATGGGTACCT GCAGCTGAGC TACGACCTGG GCTCCCAGCC CGTGGTGCTG 
CGCTCCACTG TGAAGGTCAA CACCAATCAC TGGCTGCGGG TTAAGGCTCA CAGGGAGCAG 
AGGGAAGGGT CCCTTCAGGT GGGCAATGAA GCCCCTGTGA CTGGCTCGTC TCCACTGGGC 
TCCACACAGC TGGACACAGA TGGAGCCCTG TGGCTCGGGG GCCTTCAGAA GCTGCCTGTG 
GGCCAGGCCC TGCCCAAGGC CTACAGTACA GGCTTCGTGG GCTGCTTACG GGATGTAGTG 
GTGAGCGGGC GCCCACTGCA CCTGCTGGAA GATGCAGTCA CCAGGCCAGA GCTGCGGCCC 
TGCCCTGCAC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_023565392.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_023421160.1 Cavia porcellus agrin (Agrn), transcript variant X4, mRNA.

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CloneID	ODo41203
Clone ID Related Accession (Same CDS sequence)	XM_023565392.1
Accession Version	XM_023565392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCCCGGAAGC TCCCGATTCC GGGTCCTTTC ACAGCGAGAA GGCGCTGCAG 
AGCAACCACT TCGAGCTGAG CCTGCGCACC GAGGCCACTC AGGGGCTGGT GCTGTGGAGC 
GGCAAGGCCA CAGAGCGTGC AGACTATGTG GCTCTGGCCA TCGTGGATGG GTACCTGCAG 
CTGAGCTACG ACCTGGGCTC CCAGCCCGTG GTGCTGCGCT CCACTGTGAA GGTCAACACC 
AATCACTGGC TGCGGGTTAA GGCTCACAGG GAGCAGAGGG AAGGGTCCCT TCAGGTGGGC 
AATGAAGCCC CTGTGACTGG CTCGTCTCCA CTGGGCTCCA CACAGCTGGA CACAGATGGA 
GCCCTGTGGC TCGGGGGCCT TCAGAAGCTG CCTGTGGGCC AGGCCCTGCC CAAGGCCTAC 
AGTACAGGCT TCGTGGGCTG CTTACGGGAT GTAGTGGTGA GCGGGCGCCC ACTGCACCTG 
CTGGAAGATG CAGTCACCAG GCCAGAGCTG CGGCCCTGCC CTGCACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023421160.1
CDS	188..6058
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESPEAPDSGSFHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_023565392.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023565392.1

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCCCGGAAGC TCCCGATTCC GGGTCCTTTC ACAGCGAGAA GGCGCTGCAG 
AGCAACCACT TCGAGCTGAG CCTGCGCACC GAGGCCACTC AGGGGCTGGT GCTGTGGAGC 
GGCAAGGCCA CAGAGCGTGC AGACTATGTG GCTCTGGCCA TCGTGGATGG GTACCTGCAG 
CTGAGCTACG ACCTGGGCTC CCAGCCCGTG GTGCTGCGCT CCACTGTGAA GGTCAACACC 
AATCACTGGC TGCGGGTTAA GGCTCACAGG GAGCAGAGGG AAGGGTCCCT TCAGGTGGGC 
AATGAAGCCC CTGTGACTGG CTCGTCTCCA CTGGGCTCCA CACAGCTGGA CACAGATGGA 
GCCCTGTGGC TCGGGGGCCT TCAGAAGCTG CCTGTGGGCC AGGCCCTGCC CAAGGCCTAC 
AGTACAGGCT TCGTGGGCTG CTTACGGGAT GTAGTGGTGA GCGGGCGCCC ACTGCACCTG 
CTGGAAGATG CAGTCACCAG GCCAGAGCTG CGGCCCTGCC CTGCACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_023565393.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_023421161.1 Cavia porcellus agrin (Agrn), transcript variant X5, mRNA.

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CloneID	ODo41204
Clone ID Related Accession (Same CDS sequence)	XM_023565393.1
Accession Version	XM_023565393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	agrin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176233.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCAAACTGGC CAATGAGATC CCATTCGAGA AGGCGCTGCA GAGCAACCAC 
TTCGAGCTGA GCCTGCGCAC CGAGGCCACT CAGGGGCTGG TGCTGTGGAG CGGCAAGGCC 
ACAGAGCGTG CAGACTATGT GGCTCTGGCC ATCGTGGATG GGTACCTGCA GCTGAGCTAC 
GACCTGGGCT CCCAGCCCGT GGTGCTGCGC TCCACTGTGA AGGTCAACAC CAATCACTGG 
CTGCGGGTTA AGGCTCACAG GGAGCAGAGG GAAGGGTCCC TTCAGGTGGG CAATGAAGCC 
CCTGTGACTG GCTCGTCTCC ACTGGGCTCC ACACAGCTGG ACACAGATGG AGCCCTGTGG 
CTCGGGGGCC TTCAGAAGCT GCCTGTGGGC CAGGCCCTGC CCAAGGCCTA CAGTACAGGC 
TTCGTGGGCT GCTTACGGGA TGTAGTGGTG AGCGGGCGCC CACTGCACCT GCTGGAAGAT 
GCAGTCACCA GGCCAGAGCT GCGGCCCTGC CCTGCACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023421161.1
CDS	189..6050
Translation	MWCQEDTDALCSIRARVCDESKSSWKEKSRSSEDPESMCASHSEVILVGFGNSHRPCSDRFWPTPVCRGMLCGFGAVCEPSIEDPGRASCVCKKNACPAVVAPVCGSDASTYSNECELQRAQCVLQRRIRLLRRGPCGFRDPCTNVTCSFGSTCARSADGQTATCLCPVTCHGAPEGTVCGSDGANYPSECQLLHHACTRQENISKKFDGPCDPCQEAFTDLSHICRVNPRTRRPEMLLRPDSCPPRHSPVCGDDGVTYENDCIMGRTGAVQGLLLQKVRSGQCQPRDQCPEACQFGAVCLSRRGRPRCSCDRVTCDGAYKPVCAQDGHTYDNDCLRQQAECRQQRAIPPKHQGLCAQAPSPCHEVQCAFGATCAVKNGKAVCECQQVCTGIYDPVCGSDGITYGSMCELGAMACALGQEIRVTRRGPCDQCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTQQVDLHVISAGPCQTCKDTVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGITYPSACELREAACRQQVQIEEARAGPCEQAECGSGGSGSGEEGECERELCQQRGGIWDEDSEDGPCVCDFSCQSVLRSPVCGSDGVTYGTECDLKKARCESQRELYVAAQGPCHSPTLAPLPPVFLSHCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYSGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDNLSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGQTLGPTGCEAGSLMPKTCVEMHCEFGASCVEEAGSARCACPALTCPEANATKVCGSDGITYGNECQLKTIACRQGLDIFTQSVGPCQDAVALGVYPTSVSMTTLEHVLNEALPSPSSALSLAPSSIPRSQATPLPTSRPWTTSSIPRPTARPGPVLTMPPTTTSTTASLPASAFGESGSTNGSGDEELSGDQEASGGGSGGLELPMGNNIVTAGPPIERASCYNSPLGCCSDGKTPSLDAESSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIENSLDDLFRNSDVKKDFRSVRLRDLGPGRSVRAIVDVHFDPTTAFRAPDVGQALLRQIQASRRRTLLVRRPLHEHVRFMDFDWFPAFFTGAATGATAAVATARATTTGQLPSAVTPRAYPSHTSRPTGKTTAPLTTRRPLTTLPSRMAGHQPIPPDPQQPLRPCASQPCLYGGTCWDQDSGQGFTCSCPAGRGGIICEEVLRPSLPAFGGHSFLAFPTLRAYHTLRLALEFRTLELQGLLLYNGNNRGKDFLALALQGGRVQLRFDTGSGPAVLTSSVPVKLGRWHRLELSRHWRQGTLSVDGESPVVGESPSGTDGLNLDTDLFVGGVPEDQAAVVLERTFVAVGLKGCIRLLDINNQRLELGSWQGASTQSSGVGECGENPCVPNPCSSGAPCQALDDGMFHCQCPPGRFGLTCASEKNPCQPNPCHGAAPCRVLPEGGPKCECPLGRGGALCQTDLEKDHGSQPFMADFSGFSYLELKGLYTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGRGDFVSLALRDWHLEFRYDLGKGPAVIRSKEPVAVDTWTRVSLERNGRKGAMRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSRLARAAAVSSGFDGAIQLVSLGGRQLLTQEHVLQAVGISSFADHPCTQASGHPCLNGASCLPQEATYECLCPSGFSGLHCEKGLVEKSAGDLDTLAFDGQTYIEYLNAVRESKLANEIPFEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGYLQLSYDLGSQPVVLRSTVKVNTNHWLRVKAHREQREGSLQVGNEAPVTGSSPLGSTQLDTDGALWLGGLQKLPVGQALPKAYSTGFVGCLRDVVVSGRPLHLLEDAVTRPELRPCPAP

Target ORF information:

RefSeq Version	XM_023565393.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus agrin (Agrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023565393.1

ORF Insert Sequence:

ATGTGGTGTC AGGAGGATAC AGATGCTCTC TGCAGCATTA GGGCACGGGT GTGTGATGAG 
AGCAAGTCCT CCTGGAAGGA AAAATCCCGT AGCTCTGAGG ACCCTGAAAG TATGTGTGCA 
AGCCATTCGG AGGTGATACT GGTGGGGTTT GGGAATTCAC ACAGGCCCTG CTCTGACCGC 
TTCTGGCCAA CTCCAGTGTG CAGGGGAATG CTGTGTGGTT TTGGTGCCGT GTGTGAGCCT 
AGCATAGAAG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAATGCCTG CCCAGCTGTG 
GTAGCACCTG TGTGCGGTTC AGATGCTTCT ACCTACAGCA ATGAGTGTGA GTTGCAACGT 
GCACAGTGTG TTCTACAACG GCGCATCCGC CTGCTCCGCC GAGGGCCCTG TGGGTTCAGG 
GACCCCTGCA CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCGCTC GGCTGATGGG 
CAGACAGCCA CATGCCTGTG CCCAGTTACC TGCCATGGGG CCCCAGAGGG CACCGTGTGT 
GGCAGTGATG GTGCCAATTA CCCTAGTGAA TGCCAGCTGC TGCACCATGC CTGCACACGC 
CAGGAAAACA TTTCCAAGAA GTTCGATGGC CCCTGTGACC CTTGCCAGGA AGCCTTTACC 
GACTTGAGCC ACATCTGTCG TGTGAACCCA CGCACACGGC GCCCAGAGAT GCTTCTGCGG 
CCAGACAGCT GCCCTCCCCG GCACTCGCCC GTGTGTGGAG ACGACGGGGT CACTTATGAA 
AATGACTGCA TCATGGGCCG CACTGGGGCA GTCCAGGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GTCAGCCTCG AGACCAGTGC CCTGAGGCTT GCCAGTTTGG TGCCGTGTGT 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGTGATCGTG TCACCTGTGA TGGGGCCTAC 
AAGCCTGTGT GCGCCCAGGA TGGACACACA TACGACAATG ACTGTTTGCG CCAGCAAGCC 
GAGTGTCGGC AGCAGCGTGC TATACCCCCC AAGCACCAGG GCTTGTGTGC CCAGGCCCCA 
TCCCCGTGCC ATGAGGTGCA GTGTGCATTC GGGGCGACGT GTGCTGTAAA GAATGGGAAG 
GCTGTATGTG AGTGCCAGCA GGTGTGCACA GGCATCTATG ACCCCGTGTG TGGCAGCGAT 
GGCATCACGT ATGGCAGCAT GTGCGAGCTG GGGGCCATGG CCTGTGCCCT GGGGCAGGAG 
ATCCGGGTGA CCCGCCGCGG ACCCTGTGAC CAGTGTGGAC AGTGTCGTTT TGGAGCCCTG 
TGTGAGGCCG AAACCGGGCG CTGTGTGTGC CCCTCTGAGT GTGTGGCCTC AGCCCAGCCT 
GTGTGTGGTT CTGATGGGCA CACATATGCC AGTGAATGTG AGCTTCATGT CCACGCCTGT 
ACACAGCAGG TTGATCTGCA CGTGATCTCA GCTGGACCCT GCCAGACCTG TAAGGATACA 
GTGTGTGCCT TTGGGGCAGT GTGCTCAGCT GGGCAGTGCG TGTGTCCTCG GTGTGAGCAC 
CCCCCACCAG GCCCCGTGTG CGGCAGTGAT GGTATCACCT ACCCCAGTGC ATGTGAGCTG 
CGGGAAGCTG CCTGCCGGCA GCAGGTACAA ATTGAAGAAG CCCGGGCAGG ACCCTGTGAG 
CAAGCTGAAT GTGGCTCAGG AGGCTCTGGC TCTGGAGAGG AAGGCGAGTG TGAGCGGGAA 
CTGTGCCAGC AGCGTGGTGG CATCTGGGAT GAGGACTCAG AGGATGGGCC ATGTGTCTGT 
GACTTCAGCT GTCAGAGTGT CCTGAGGAGC CCGGTATGCG GCTCAGATGG GGTTACCTAT 
GGCACTGAGT GTGACCTGAA GAAGGCCAGG TGTGAATCAC AACGAGAACT GTACGTCGCG 
GCTCAGGGGC CCTGCCACAG TCCTACATTG GCACCACTGC CACCTGTGTT TCTTTCACAC 
TGTGCCCAGA CCCCATATGG CTGCTGCCAG GACAATGTCA CTGCTGCCAG GGGTGTGGGC 
CTGGCTGGCT GCCCCAGCAC CTGCCAGTGC AACCCACATG GCTCCTACAG TGGCACCTGT 
GACCCAGCCA CAGGGCAGTG CTCCTGCCGA CCAGGCGTGG GAGGCCTTAA GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA TTTCCGTGGC ATTGTCACTG ACAACCTGAG TGGCTGTACT 
CCCTGCAGCT GTGACCCCCA GGGTGCTGTG CGGGACGACT GTGAGCAGAT GACTGGACTG 
TGCTCCTGCA AGCCAGGAGT GGCTGGGCCC AAGTGTGGGC AGTGTCCAGA TGGCCAAACC 
CTGGGCCCTA CTGGTTGTGA AGCAGGCTCC TTGATGCCCA AGACCTGTGT GGAGATGCAC 
TGTGAGTTTG GAGCTTCCTG TGTGGAAGAA GCTGGTTCTG CCCGCTGTGC CTGCCCAGCC 
CTCACGTGCC CAGAGGCCAA TGCCACTAAG GTCTGTGGGT CAGATGGTAT CACATACGGC 
AATGAGTGCC AGCTGAAGAC GATTGCCTGC CGCCAGGGCC TGGACATCTT CACCCAGAGT 
GTTGGCCCAT GCCAGGATGC TGTTGCTCTT GGCGTATACC CCACATCTGT ATCCATGACC 
ACCCTGGAGC ATGTCCTGAA TGAGGCGCTG CCGTCCCCCT CCAGTGCCCT TTCCCTGGCA 
CCCAGCAGTA TTCCCCGCAG CCAGGCTACT CCTCTACCCA CATCACGACC CTGGACCACA 
TCCAGCATCC CCAGGCCCAC GGCAAGACCT GGGCCTGTGC TAACCATGCC CCCCACAACC 
ACCTCCACCA CTGCCAGCCT TCCCGCATCA GCCTTCGGTG AATCTGGCAG CACCAATGGG 
AGTGGTGATG AGGAGCTCAG CGGAGACCAG GAGGCCAGTG GGGGAGGCTC TGGGGGACTT 
GAGCTCCCCA TGGGCAACAA CATTGTGACT GCTGGGCCAC CCATCGAGAG GGCTTCCTGC 
TACAACTCTC CTTTGGGCTG CTGTTCAGAT GGCAAGACAC CCTCACTGGA TGCAGAGAGC 
TCCAACTGCC CTGCCACCAA GGCGTTCCAG GGCGTACTGG AGCTGGAGGG GGTCGAGGGG 
CAGGAGCTGT TCTACACACC TGAGATGGCT GACCCCAAGT CAGAACTGTT TGGGGAAACT 
GCCAGGAGCA TTGAGAACTC GCTGGATGAC CTGTTCCGAA ACTCAGATGT CAAGAAGGAC 
TTCCGGAGTG TCCGCCTGCG GGACCTGGGG CCTGGCAGAT CGGTCCGAGC TATTGTGGAT 
GTGCACTTTG ACCCCACTAC GGCCTTCAGG GCTCCCGATG TGGGCCAGGC CCTCCTCCGG 
CAGATCCAGG CATCCAGACG CCGGACCTTA CTGGTGAGGA GGCCTCTTCA TGAACATGTG 
CGATTCATGG ACTTTGACTG GTTTCCAGCC TTCTTCACAG GAGCTGCTAC AGGAGCCACG 
GCTGCAGTGG CCACAGCCAG AGCCACCACC ACAGGCCAGC TGCCCTCTGC TGTAACCCCT 
CGGGCCTACC CCAGTCACAC CAGCCGACCT ACTGGCAAGA CCACAGCACC CCTCACCACA 
CGCCGGCCTC TCACTACGCT CCCCAGCCGC ATGGCAGGAC ACCAACCCAT TCCCCCAGAC 
CCTCAGCAGC CACTGAGGCC CTGTGCCTCC CAGCCCTGCC TCTATGGGGG CACGTGCTGG 
GACCAGGACT CTGGCCAAGG CTTCACTTGC AGCTGCCCAG CGGGCAGGGG CGGCATCATC 
TGCGAGGAGG TATTGCGCCC CTCTCTGCCT GCTTTTGGAG GCCACTCCTT CCTGGCCTTC 
CCCACCCTAC GCGCCTACCA CACACTGCGC CTGGCACTAG AATTCCGAAC ACTGGAGCTG 
CAGGGGCTGC TGCTCTACAA TGGGAACAAC CGTGGCAAAG ACTTCTTGGC GCTGGCTCTA 
CAGGGTGGGC GGGTGCAGCT CAGGTTTGAC ACAGGTTCTG GGCCAGCAGT GTTGACCAGT 
TCCGTGCCTG TGAAACTGGG CCGGTGGCAC CGCCTAGAAC TGTCAAGGCA TTGGCGCCAG 
GGCACACTGT CTGTGGATGG CGAGAGCCCT GTTGTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTTAACC TGGACACGGA CCTTTTTGTG GGTGGCGTCC CAGAGGACCA GGCTGCTGTG 
GTGCTTGAGC GGACCTTCGT TGCAGTTGGC CTGAAAGGCT GCATCCGGTT GCTGGACATC 
AACAACCAGC GGCTGGAGCT GGGCAGCTGG CAAGGAGCTT CGACCCAGAG CTCTGGCGTG 
GGCGAGTGTG GGGAAAACCC CTGTGTGCCC AACCCCTGCA GCAGTGGGGC GCCATGCCAG 
GCACTTGACG ATGGCATGTT CCACTGTCAG TGCCCACCTG GCCGCTTTGG TCTGACCTGT 
GCCAGTGAGA AGAACCCCTG CCAGCCAAAC CCTTGCCATG GAGCAGCCCC TTGCCGTGTA 
CTACCCGAGG GAGGGCCGAA GTGCGAGTGC CCCCTTGGGC GTGGGGGCGC CCTCTGCCAG 
ACAGACTTGG AGAAGGACCA TGGCTCCCAG CCCTTCATGG CGGATTTCAG CGGCTTCTCC 
TATCTGGAGC TGAAAGGCCT GTACACCTTT GAAAGAGACC TGGGAGAAAA GATGGCGCTG 
GAGGTTGTGT TCCTGGCCCG TGGCCCCAGT GGCTTGCTCC TTTACAATGG GCAGAAGACA 
GATGGCAGAG GAGACTTTGT GTCGCTGGCC CTGCGTGACT GGCATCTGGA ATTCCGTTAT 
GACCTGGGCA AGGGCCCTGC AGTCATCAGG AGCAAGGAGC CAGTCGCCGT GGACACCTGG 
ACAAGGGTCT CACTGGAGCG CAATGGCCGC AAGGGTGCCA TGCGTGTGGG CGATGGGCCC 
CGAGTGCTGG GGGAGTCCCC GAAATCCCGC AAGGTGCCTC ACACTGTGCT CAACCTGAAG 
GAGCCACTCT ATGTGGGGGG CGCCCCTGAT TTCAGTAGGT TGGCCCGGGC AGCTGCGGTG 
TCCTCTGGCT TTGATGGTGC CATCCAGCTG GTTTCCCTGG GAGGCCGCCA GTTGCTGACC 
CAGGAACATG TGCTGCAGGC AGTGGGCATC TCCTCCTTTG CAGACCACCC TTGCACCCAA 
GCCTCGGGCC ACCCCTGTCT TAATGGAGCC TCGTGTCTCC CCCAGGAAGC CACCTATGAG 
TGCCTGTGTC CCTCAGGCTT CTCAGGGCTG CACTGTGAGA AGGGACTGGT TGAAAAGTCG 
GCGGGGGACC TGGACACATT GGCCTTTGAT GGGCAGACCT ACATCGAGTA CCTCAATGCT 
GTGAGGGAGA GCAAACTGGC CAATGAGATC CCATTCGAGA AGGCGCTGCA GAGCAACCAC 
TTCGAGCTGA GCCTGCGCAC CGAGGCCACT CAGGGGCTGG TGCTGTGGAG CGGCAAGGCC 
ACAGAGCGTG CAGACTATGT GGCTCTGGCC ATCGTGGATG GGTACCTGCA GCTGAGCTAC 
GACCTGGGCT CCCAGCCCGT GGTGCTGCGC TCCACTGTGA AGGTCAACAC CAATCACTGG 
CTGCGGGTTA AGGCTCACAG GGAGCAGAGG GAAGGGTCCC TTCAGGTGGG CAATGAAGCC 
CCTGTGACTG GCTCGTCTCC ACTGGGCTCC ACACAGCTGG ACACAGATGG AGCCCTGTGG 
CTCGGGGGCC TTCAGAAGCT GCCTGTGGGC CAGGCCCTGC CCAAGGCCTA CAGTACAGGC 
TTCGTGGGCT GCTTACGGGA TGTAGTGGTG AGCGGGCGCC CACTGCACCT GCTGGAAGAT 
GCAGTCACCA GGCCAGAGCT GCGGCCCTGC CCTGCACCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Agrn
Entrez Gene ID	100716304
Full Name	agrin
Gene Type	protein-coding
Organism	Cavia porcellus(domestic guinea pig)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Agrn cDNA ORF clone, Cavia porcellus(domestic guinea pig)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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