ILS1 cDNA ORF clone, Candida albicans SC5314

Gene
Clones
gRNAs

Summary

Gene Symbol	ILS1
Entrez Gene ID	3645956
Full Name	isoleucine--tRNA ligase
Synonyms	CaO19.2138
Gene Type	protein-coding
Organism	Candida albicans SC5314

mRNA and Protein(s)

mRNA	Protein	Name
XM_707320.2	XP_712413.1	isoleucine--tRNA ligase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly of a phased diploid Candida albicans genome facilitates allele-specific measurements and provides a simple model for repeat and indel structure.
Muzzey D, Schwartz K, Weissman JS, Sherlock G
Genome biology14(9)R97(2013)

Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes.
van het Hoog M, Rast TJ, Martchenko M, Grindle S, Dignard D, Hogues H, Cuomo C, Berriman M, Scherer S, Magee BB, Whiteway M, Chibana H, Nantel A, Magee PT
Genome biology8(4)R52(2007)

The diploid genome sequence of Candida albicans.
Jones T, Federspiel NA, Chibana H, Dungan J, Kalman S, Magee BB, Newport G, Thorstenson YR, Agabian N, Magee PT, Davis RW, Scherer S
Proceedings of the National Academy of Sciences of the United States of America101(19)7329-34(2004 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ILS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ILS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCg12110	XM_707320.2 Latest version!	Candida albicans SC5314 isoleucine--tRNA ligase (ILS1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ILS1 ( XM_707320.2 ) cDNA ORF clone, Candida albicans SC5314 -> XP_712413.1 Candida albicans SC5314 isoleucine--tRNA ligase (ILS1), partial mRNA.

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CloneID	OCg12110
Clone ID Related Accession (Same CDS sequence)	XM_707320.2
Accession Version	XM_707320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1522684800000
Organism	Candida albicans SC5314
Product	isoleucine--tRNA ligase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_032094). On Dec 8, 2016 this sequence version replaced XM_707320.1. ##Genome-Annotation-Data-START## Annotation Provider :: CGD Annotation Status :: Full annotation Annotation Version :: A22-s07-m01-r01 URL :: http://www.candidagenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCGTTAC AAGAAAGCAA CAACAATATC CCTCAAGGTG CTTTTAGTTT TCCTAAGGAA 
GAAGAAGCAG TTATCAAACA TTGGGATGAT GTCAATGCTT TTCAAAGAAC TTTAGAGTTG 
ACTGAAGATT TACCGCCATT TGCGTTTTTT GACGGACCAC CATTTGCCAC TGGTACTCCT 
CATTACGGGC ACATTTTGGC CTCTACAGTC AAAGATATTA TCCCACGTTA TGCCACCATG 
AACGGGTATC ATGTGGAGAG AAGATTCGGT TGGGATACCC ACGGTTTGCC AGTAGAACAT 
GAAATTGACA AAAAGTTGAA CATTACCTCG AAAGAAGATG TTTATGCCAT GGGTATTGAC 
AAGTACAATG CTGAATGTCG TGCAATTGTG ATGAGATACG CTGATGAATG GCGTAGAACA 
ATCAAGAGAT TGGGGAGATG GATTGATATG GACAACGATT ACAAAACCTT GTACCCTGAA 
TTTATGGAAT CTGTGTGGTG GGCTTTCAAG GAGTTGTTTA ACAAGGATGC CGTTTATAGA 
GGTTTGAGGG TCATGCCTTA TTCCACTGCT TGTACCACAC CATTGTCGAA CTTTGAAGCC 
CAACAAAACT ATAAAGAAGT TAACGACCCA GCACTTACTA TTTCGTTCCC ATTGCTTGAT 
AACGAAGACA CTTGTTTGGT TGCTTGGACT ACCACGCCAT GGACCTTACC TGCAAATCTT 
GCGTTAGCAG TTAATCCAAA GTTTGAGTAT GTAAAGATTT TTGATGAGGA AAAAAAGAAA 
AACTTTATTC TTTTGGAAAG TTTGATCAGT ACTTTGTACA AGAAACCTAA ATCGGCCAAG 
TTCAAGGTTG TTGAGAAAAT TTTGGGTAAA GATTTAGTTG GACTCAAATA CAAGCCATTG 
TTCAATTACT TTTACGAAGA TTTCAAGGAT ACTGGGTTCA GAGTTATTCC AGCCGACTAT 
GTTACCAACG ATTCTGGTAC TGGTATTGTC CATCAAGCCC CATCCTATGG TGAAGAGGAT 
TTCAACAGTA CCAAAGCCGC AGGAGTCATC AACGAAAAGA AGTTGCCACC AAGCATTGTT 
GATGATTCAG GGAGAATGGA ATCCAATGTT CCTGAAATTG CCGGAATGTA CTTTAAGGAT 
GCCGACAAGG TTATTATCAA GAAATTGCTG GAAGAAGGTA GACTCTTGGT CAACACCCAA 
GTAAAGCACT CGTACCCATT CTGTTGGAGA TCAGATACTC CATTGATGTA CAGAACCGTC 
CCTGCATGGT TTGTTAGAAT TGGCGAAGTC ATTCCTGAAA TGTTGGATAA TGTTGAAAAG 
ACAAACTGGG TTCCTTCCAA CATTAAAGAT AAGAGATTTT CCAACTGGAT TGCCAATGCC 
AGAGACTGGA ACATTTCCAG AAATAGATAC TGGGGTACAC CAATTCCATT ATGGGTTTCT 
GACGATTTTG AAGAAATGGT GTGTGTTGGT TCTATCCAAG AATTAAGGGA GTTGTCTGGT 
CGTGATGATA TTACTGATAT TCACCGTGAG AGCATCGATT CTATTACCAT CCCATCCAAA 
AAGGGTAAGG GCCAATTGAA GAGAATTGAA GAAGTTTTTG ATTGTTGGTT TGAATCTGGT 
TCTATGCCAT ATGCATCCAA ACATTATCCA TTTGAAAATG AAAAGAAGTT TTTGGATGCC 
TTTCCGGCAA ATTTCATTTC CGAAGGTTTA GATCAAACTA GAGGTTGGTT CTACACATTG 
ACTGTATTGG GTACCCATTT GTTCAAAACC GCACCATATC AGAATGTTAT TGTCACTGGT 
ATTGTGTTGG CTGCTGATGG TAAAAAGATG TCGAAACGTT TGAAGAACTA CCCAGACCCA 
ACCTTGGTGT TGGAGAAATA TGGTGCCGAT GCGTTGAGAT TGTACTTGAT CAATTCCCCA 
GTGTTGAGAG CCGAAACATT AAAGTTTAAG GAAGAAGGGG TCAAGGAAAT TGTTTCCAGT 
GTGTTGTTGC CATGGTACAA CTCCTACAAG TTTTTAAAGG ATGCTGCTGA CCTTTTCAAG 
AAGGATAATG GCAAAGACTT TGTTTACGAC AACAGTTTAC ATTCAACCAA CGTTATGGAC 
AGATGGTTAT TAGCATCAAT CCAATCTTTG ATCAAGTTTA TTCACGAAGA AATGACTGGG 
TACAGATTAT ATACTGTTGT TCCTAGATTG TTGCATTTCA TTGACGATTT GACCAACTGG 
TACATTAGAT TCAACCGTCG TAGAATCAAG GGATATGCTT CCGAAGATGT TGAAGACACC 
CAAAAGGGTC TCAATACATT GGTCGAAGCG TTGTTGACAT TGTCTAGAGC AATGGCTCCT 
TTCACTCCAT ACTTGGCTGA TGGAATTTAC CAAAGAATCA AGGTATACTT TAAGCAAGAA 
GATTTGGAAA AGATTGCTAT TAACCCTAAG AATGTTGACT TGAGATCAGT GCATTTCTTG 
AGCTACCCAT CAGTGAGACA AGAGTTGTTT GATGAAAAGA TTGAGGTTGC TGTTGCAAGA 
ATGCAAAAGG TTATTGACAT GGCCAGAAAC ATTAGAGAAA AGAAGATGAT TTCATTAAAG 
ACTCCATTGA ATGAGTTGGT GGTTTTGAGT GCAGATGCTG ATTTGTTGAA GGACATTGAT 
TCTTTGAAAG GATACATTAG TGATGAGTTG AATGTGAGAA ATGTTGTCAT CACATCAGAC 
GAAGCCAAGT ACTGTGTTGA GTACTCGTGT GTTGCTGATT GGCCAGTGTT GGGTAAAAAG 
TTAAAGTCAG ACGCCAAAAA AGTCAAGGCA GCATTGCCTA AAGTTTCCTC TGAAGAAGTT 
CAAAGATTTG CTGAGTGTGG TAAAATCACT GTTGATGGTA TTGACTTGGT GACTGAAGAT 
TTGCAAGTGC AAAGAGGCTT GCCAGCTTCT AAAGCCGAAG AGGGCCAAGA GTTTAGATCT 
CACCAAGATG TTTTGATTAT TTTGGATGTC AATTTGCACC CTGAATTGGA AAGTGAAGGT 
TTGGCAAGAG AGTTGATTAA CCGTATCCAA AGATTGCGTA AAAAGGCTGG TTTGAATACC 
ACTGACGATG TTCAAGTTCA ATACCGTGTC GTCAAAGATA CTATTGATTT GCCAAAGATT 
ATTAAAGACA ATGAAGAGTT GTTGTTGAAA TCAACCAAAT ATCCAATTGA GGAATTGAAG 
GAAGCACAAG ATCTGGCTAA TGTCATTACC GATGAAGAGC AAACAATTAA CGATACAGTG 
TTTAATTTGC GTTTATTAAA AATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_712413.1
CDS	1..3267
Translation	MSLQESNNNIPQGAFSFPKEEEAVIKHWDDVNAFQRTLELTEDLPPFAFFDGPPFATGTPHYGHILASTVKDIIPRYATMNGYHVERRFGWDTHGLPVEHEIDKKLNITSKEDVYAMGIDKYNAECRAIVMRYADEWRRTIKRLGRWIDMDNDYKTLYPEFMESVWWAFKELFNKDAVYRGLRVMPYSTACTTPLSNFEAQQNYKEVNDPALTISFPLLDNEDTCLVAWTTTPWTLPANLALAVNPKFEYVKIFDEEKKKNFILLESLISTLYKKPKSAKFKVVEKILGKDLVGLKYKPLFNYFYEDFKDTGFRVIPADYVTNDSGTGIVHQAPSYGEEDFNSTKAAGVINEKKLPPSIVDDSGRMESNVPEIAGMYFKDADKVIIKKLLEEGRLLVNTQVKHSYPFCWRSDTPLMYRTVPAWFVRIGEVIPEMLDNVEKTNWVPSNIKDKRFSNWIANARDWNISRNRYWGTPIPLWVSDDFEEMVCVGSIQELRELSGRDDITDIHRESIDSITIPSKKGKGQLKRIEEVFDCWFESGSMPYASKHYPFENEKKFLDAFPANFISEGLDQTRGWFYTLTVLGTHLFKTAPYQNVIVTGIVLAADGKKMSKRLKNYPDPTLVLEKYGADALRLYLINSPVLRAETLKFKEEGVKEIVSSVLLPWYNSYKFLKDAADLFKKDNGKDFVYDNSLHSTNVMDRWLLASIQSLIKFIHEEMTGYRLYTVVPRLLHFIDDLTNWYIRFNRRRIKGYASEDVEDTQKGLNTLVEALLTLSRAMAPFTPYLADGIYQRIKVYFKQEDLEKIAINPKNVDLRSVHFLSYPSVRQELFDEKIEVAVARMQKVIDMARNIREKKMISLKTPLNELVVLSADADLLKDIDSLKGYISDELNVRNVVITSDEAKYCVEYSCVADWPVLGKKLKSDAKKVKAALPKVSSEEVQRFAECGKITVDGIDLVTEDLQVQRGLPASKAEEGQEFRSHQDVLIILDVNLHPELESEGLARELINRIQRLRKKAGLNTTDDVQVQYRVVKDTIDLPKIIKDNEELLLKSTKYPIEELKEAQDLANVITDEEQTINDTVFNLRLLKI

Target ORF information:

RefSeq Version	XM_707320.2
Organism	Candida albicans SC5314
Definition	Candida albicans SC5314 isoleucine--tRNA ligase (ILS1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_707320.2

ORF Insert Sequence:

ATGTCGTTAC AAGAAAGCAA CAACAATATC CCTCAAGGTG CTTTTAGTTT TCCTAAGGAA 
GAAGAAGCAG TTATCAAACA TTGGGATGAT GTCAATGCTT TTCAAAGAAC TTTAGAGTTG 
ACTGAAGATT TACCGCCATT TGCGTTTTTT GACGGACCAC CATTTGCCAC TGGTACTCCT 
CATTACGGGC ACATTTTGGC CTCTACAGTC AAAGATATTA TCCCACGTTA TGCCACCATG 
AACGGGTATC ATGTGGAGAG AAGATTCGGT TGGGATACCC ACGGTTTGCC AGTAGAACAT 
GAAATTGACA AAAAGTTGAA CATTACCTCG AAAGAAGATG TTTATGCCAT GGGTATTGAC 
AAGTACAATG CTGAATGTCG TGCAATTGTG ATGAGATACG CTGATGAATG GCGTAGAACA 
ATCAAGAGAT TGGGGAGATG GATTGATATG GACAACGATT ACAAAACCTT GTACCCTGAA 
TTTATGGAAT CTGTGTGGTG GGCTTTCAAG GAGTTGTTTA ACAAGGATGC CGTTTATAGA 
GGTTTGAGGG TCATGCCTTA TTCCACTGCT TGTACCACAC CATTGTCGAA CTTTGAAGCC 
CAACAAAACT ATAAAGAAGT TAACGACCCA GCACTTACTA TTTCGTTCCC ATTGCTTGAT 
AACGAAGACA CTTGTTTGGT TGCTTGGACT ACCACGCCAT GGACCTTACC TGCAAATCTT 
GCGTTAGCAG TTAATCCAAA GTTTGAGTAT GTAAAGATTT TTGATGAGGA AAAAAAGAAA 
AACTTTATTC TTTTGGAAAG TTTGATCAGT ACTTTGTACA AGAAACCTAA ATCGGCCAAG 
TTCAAGGTTG TTGAGAAAAT TTTGGGTAAA GATTTAGTTG GACTCAAATA CAAGCCATTG 
TTCAATTACT TTTACGAAGA TTTCAAGGAT ACTGGGTTCA GAGTTATTCC AGCCGACTAT 
GTTACCAACG ATTCTGGTAC TGGTATTGTC CATCAAGCCC CATCCTATGG TGAAGAGGAT 
TTCAACAGTA CCAAAGCCGC AGGAGTCATC AACGAAAAGA AGTTGCCACC AAGCATTGTT 
GATGATTCAG GGAGAATGGA ATCCAATGTT CCTGAAATTG CCGGAATGTA CTTTAAGGAT 
GCCGACAAGG TTATTATCAA GAAATTGCTG GAAGAAGGTA GACTCTTGGT CAACACCCAA 
GTAAAGCACT CGTACCCATT CTGTTGGAGA TCAGATACTC CATTGATGTA CAGAACCGTC 
CCTGCATGGT TTGTTAGAAT TGGCGAAGTC ATTCCTGAAA TGTTGGATAA TGTTGAAAAG 
ACAAACTGGG TTCCTTCCAA CATTAAAGAT AAGAGATTTT CCAACTGGAT TGCCAATGCC 
AGAGACTGGA ACATTTCCAG AAATAGATAC TGGGGTACAC CAATTCCATT ATGGGTTTCT 
GACGATTTTG AAGAAATGGT GTGTGTTGGT TCTATCCAAG AATTAAGGGA GTTGTCTGGT 
CGTGATGATA TTACTGATAT TCACCGTGAG AGCATCGATT CTATTACCAT CCCATCCAAA 
AAGGGTAAGG GCCAATTGAA GAGAATTGAA GAAGTTTTTG ATTGTTGGTT TGAATCTGGT 
TCTATGCCAT ATGCATCCAA ACATTATCCA TTTGAAAATG AAAAGAAGTT TTTGGATGCC 
TTTCCGGCAA ATTTCATTTC CGAAGGTTTA GATCAAACTA GAGGTTGGTT CTACACATTG 
ACTGTATTGG GTACCCATTT GTTCAAAACC GCACCATATC AGAATGTTAT TGTCACTGGT 
ATTGTGTTGG CTGCTGATGG TAAAAAGATG TCGAAACGTT TGAAGAACTA CCCAGACCCA 
ACCTTGGTGT TGGAGAAATA TGGTGCCGAT GCGTTGAGAT TGTACTTGAT CAATTCCCCA 
GTGTTGAGAG CCGAAACATT AAAGTTTAAG GAAGAAGGGG TCAAGGAAAT TGTTTCCAGT 
GTGTTGTTGC CATGGTACAA CTCCTACAAG TTTTTAAAGG ATGCTGCTGA CCTTTTCAAG 
AAGGATAATG GCAAAGACTT TGTTTACGAC AACAGTTTAC ATTCAACCAA CGTTATGGAC 
AGATGGTTAT TAGCATCAAT CCAATCTTTG ATCAAGTTTA TTCACGAAGA AATGACTGGG 
TACAGATTAT ATACTGTTGT TCCTAGATTG TTGCATTTCA TTGACGATTT GACCAACTGG 
TACATTAGAT TCAACCGTCG TAGAATCAAG GGATATGCTT CCGAAGATGT TGAAGACACC 
CAAAAGGGTC TCAATACATT GGTCGAAGCG TTGTTGACAT TGTCTAGAGC AATGGCTCCT 
TTCACTCCAT ACTTGGCTGA TGGAATTTAC CAAAGAATCA AGGTATACTT TAAGCAAGAA 
GATTTGGAAA AGATTGCTAT TAACCCTAAG AATGTTGACT TGAGATCAGT GCATTTCTTG 
AGCTACCCAT CAGTGAGACA AGAGTTGTTT GATGAAAAGA TTGAGGTTGC TGTTGCAAGA 
ATGCAAAAGG TTATTGACAT GGCCAGAAAC ATTAGAGAAA AGAAGATGAT TTCATTAAAG 
ACTCCATTGA ATGAGTTGGT GGTTTTGAGT GCAGATGCTG ATTTGTTGAA GGACATTGAT 
TCTTTGAAAG GATACATTAG TGATGAGTTG AATGTGAGAA ATGTTGTCAT CACATCAGAC 
GAAGCCAAGT ACTGTGTTGA GTACTCGTGT GTTGCTGATT GGCCAGTGTT GGGTAAAAAG 
TTAAAGTCAG ACGCCAAAAA AGTCAAGGCA GCATTGCCTA AAGTTTCCTC TGAAGAAGTT 
CAAAGATTTG CTGAGTGTGG TAAAATCACT GTTGATGGTA TTGACTTGGT GACTGAAGAT 
TTGCAAGTGC AAAGAGGCTT GCCAGCTTCT AAAGCCGAAG AGGGCCAAGA GTTTAGATCT 
CACCAAGATG TTTTGATTAT TTTGGATGTC AATTTGCACC CTGAATTGGA AAGTGAAGGT 
TTGGCAAGAG AGTTGATTAA CCGTATCCAA AGATTGCGTA AAAAGGCTGG TTTGAATACC 
ACTGACGATG TTCAAGTTCA ATACCGTGTC GTCAAAGATA CTATTGATTT GCCAAAGATT 
ATTAAAGACA ATGAAGAGTT GTTGTTGAAA TCAACCAAAT ATCCAATTGA GGAATTGAAG 
GAAGCACAAG ATCTGGCTAA TGTCATTACC GATGAAGAGC AAACAATTAA CGATACAGTG 
TTTAATTTGC GTTTATTAAA AATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly of a phased diploid Candida albicans genome facilitates allele-specific measurements and provides a simple model for repeat and indel structure.
Genome biology14(9)R97(2013)
Muzzey D,Schwartz K,Weissman JS,Sherlock G

Assembly of the Candida albicans genome into sixteen supercontigs aligned on the eight chromosomes.
Genome biology8(4)R52(2007)
van het Hoog M,Rast TJ,Martchenko M,Grindle S,Dignard D,Hogues H,Cuomo C,Berriman M,Scherer S,Magee BB,Whiteway M,Chibana H,Nantel A,Magee PT

The diploid genome sequence of Candida albicans.
Proceedings of the National Academy of Sciences of the United States of America101(19)7329-34(2004 May)
Jones T,Federspiel NA,Chibana H,Dungan J,Kalman S,Magee BB,Newport G,Thorstenson YR,Agabian N,Magee PT,Davis RW,Scherer S

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ILS1 cDNA ORF clone, Candida albicans SC5314

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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