BRAFLDRAFT_122749 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_122749
Entrez Gene ID	7226370
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002599435.1	XP_002599481.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_122749 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_122749 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb09746	XM_002599435.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_122749 ( XM_002599435.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002599481.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb09746
Clone ID Related Accession (Same CDS sequence)	XM_002599435.1
Accession Version	XM_002599435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101462). COMPLETENESS: incomplete on the 5' end.

ATGGACATTT TCGGCATGCA CATCACCTCC CAATGTTTGC AGGAAGAAGG AAATGGGGTG 
CCATCAACTG TACAGGTCCG GAACAGACGA AACATCAGAC AAGCCAAGAA GAGGAAAGTT 
GAGGAAACTC CTGTTTACAT TGAGGAAGGT GTATCAGGTG ATGGTCATGT GACCAATGGT 
TGCCATGGTG ATGATGATGT CACAGGAAAT GCCGACCCCT CGGCAGAAGA TGGCAGGCTC 
CTGCACGTGC TTGGGGAGGC CTCGCTTGGA CTGTTTGAGC AAAGTGGAGC AAAAGACATT 
CAGGAATCTA CAACAGTTCT TAATTCTGGA GGAAAAGTTC CAACAATAGA AGAACATAAA 
GAAGACCAGG TTGGTGAGAA GCCTCTACTG ACAAGCCATG ATCACAATGA TGGGAGTCAT 
GCTGACCAGG AACCCCATGG AGATGACCAG AAGTCGGACA CACTCGGCCC CACCATCAGC 
GAGGCAGAAC CATTGGACAT TAGCAAGGAC CAGGAACAGA TGGCCGTCGA CCTTCTGACA 
ACAGAAGTTG AAGATGAAGT TCCCGCAGAC ATAGGGAAGG AGGTAAAGGC ACAGGAGAAG 
GTAGAGTTCT GGCTGAAACA CCACAACACA ACTGCCACAG AACCAGGGGG CACAGAGGAC 
CTGGATCCAT ATGAGTTCAT AGGCAGCCAG CAGGATCATG CAAAAGGGAA GAAGAAGTCT 
AGGAAAAGGG GGAAGAAAGG AAGAAAGCCT CCTGCAGCTG ACAACAGGAA CAAGAAGTCT 
CCTAAGTCTT CTTCTGGTCC TTCTAAAGGC AAACCTACGA AGACATCAGC TAAGAAGGTT 
GAAGAAGTGG AAGATGTAGA GGTAGACTGT GAAGACTTGG ATCAGGAAGA AGGGGAGGCC 
TCAGCTGCTA GCATGACTGA CTGTTCGGAG GCATCATGGA AGCCTGGCCA GTCTGACCCG 
GATTGGGGGA AGGGAAGAAG CTCGCGACGG AAGTCTGCAG AGACAAACGC TGACAAAGAA 
TCTGTATCCA GCAAAGGTCG TAAGAGGAAG CAAAGAAATC CTGCCGACAG GGAGGACCAG 
ATGACAGTGT CTTCAGGAAG CAGCAGTCAG CTGCGCATCG ACCGATGGGT GCAGAGAAAA 
GAGAAACTGT TTGTCAAGAG CTACAGCAAA CCTGCTTCGT CCCGGCGCGA GAAAACTTTA 
CTCCATAAGA AGAAGGCTGC AGGTCTTGAG GAACAGTGGC GGAGAGCCAA GGACATGGCC 
GGAGACTTCT CCGCCAAAGT CTTGCACCCT ATCGCCGAAG AACTCCCCAG CAAGACTAAG 
AAGAAATCTA AAGACAACCA CACAACATCA AAAGCTTCTA CAAAGGAAAC CTGTAGTGCA 
ACAGGTCTAC ATCCTTTGCA GGATTTGCCA AGTCTAGGAG AGAGAGAAGA AGTACTCAAT 
AGTGAAGATG ACATGTGTGT TTACCTCACA AGCAATGGAG AACTCCTCAA CAGCCCTGAA 
AAGAGCGACA ACCCAAGTGA TGATGATCAA GATCAGGAAA AACAGCCAAC CAAGGAGAAG 
TCTGTAGCAT TGACGGAACA AGAACAGAAG ATCGCAGCAG ATCTCAATGA GCTCGATGAA 
CAGACACTCA GTCAGCTAGG CATGTTCTCA CAGCAGTACC TTCAGAAGAG GAAGAGAAGG 
AAGGATAGGA AGAAGAGACT TGAAGAGGTG CAAAATGAGG ATGAGGACAA CATCTTCGCA 
GCAATCCGGG CTTTAGAAAG TGAGAGCAAT CTTTGCAACA AGGAACATCA AACCCAAGAT 
GAAACATCAA GAAAAGAAGA TAACTGCACA GAAGTAGTGG ATGCAGGAGA TGTAATTTCA 
GCACCAGTGG ATGAAGAAGT GAAGAAGGAT AACAATTCCA GAAAGTCCCG ACAGGCAAGG 
TCTTTGTCCC GAGATCGCAG AGGTCAAGGT GATGTCACAG AGGAAAAAGA AGCAAGGCCA 
AGGCGGACAA GATCTTCTTC AAGGAAGAGT GCAGAAGATG TCCTTGTAGA TGTTGGGAAT 
GCTGTGTCCG ACAGAAGCAG TAGACAAGCA GGATCTTCAT CTAAGACTAA GACTAATGTA 
AGGAAGAATC AGAATGACAA GGAAGATGGT GAAACAAATG GTGAAGAACA GTCAAGACCA 
GATTGCATGA AGGATGAGTG TGAACATATC CCCCAAGATG CAGGAACATC TGACAAAGAT 
AAGCTTGCTA AGAAGCAGAA GGAAGAAACA GAAAGACAGC AAACTGCAAA AGAAGAAAAG 
ACATCTGAAA AGGAATTGGA AACAAGGCAA GAAGGTTCTG TCAACATTGA AGAAACTGCC 
ATGAAGGAAA ATGCAACGTC ACCAAAGGCT TTGCAGATGA GAAAACGTCA CGCTGCAGAA 
AAACAAACTA ACAAGAAAGC TCTTGGCAAC ATGGGAGAAT CGCTTGGCGT ACGACAGACA 
AGACGCAACA AGAAGATGCC TGAGACCGTG AAGGAAGCTG AACCTCCGAA GCATCTCCCT 
CTTGTGACAA CAAAAGACAC AGTTCCTCAG GATCCTTACC AGTTTCAGTA CAGCCAGGAA 
AGCAACAGAA AGAAAGCAGG AAAGAAGAAG GGAAGGAGAC AACGGGGTAA AGTGAAGAAG 
CTGAAAAGGA CAAAAGCAAG GAAAAGGGAG TTGCTGTTGA TCCAAACAGG ATGGATAGAA 
AGAGAAGAGG CTGGAGAAGA TGAAGATAGT GAAGAGGAAA ATGGTCAGGA GAATGTGGAA 
GACCAAACGG TTGACGAGTT AAATGTGGAT GAATCTAGGG ATGCCCAAGC ACACTTAGAC 
AAGCAAGAAG AAAGGGACCA GGATACAAAG GTCCTTGGTG AAGAGAAGGA TAATACAGAA 
GTATCAGTTA TTCCAGAGTC AAACCCCAAC ACAGAGCCCA TCTCAAGCAG TGTGAAGAAC 
AAAAAGCATG ATGTTGACCT TACTGCCTGT CAACCAAGTT GTTCATTGGA GGAAACTCCA 
GGCATCTACT CCACTGACAT TATCCCTCCA TCTGTAGAGG GCACTCAGAT AATGTCTCCT 
CAAGCTGGCA AAAAGAAGAG GAAGACCTCC TTGAATAAGA AGAAGACAGA GGAAGACACA 
AGTCAGAGGG AGACAGGGGA AGACACAAAA CTGTCCAAGT CTGGAAAAGT TTCATCGCCA 
GAGACGGAAA ACTCTGAGGA CATCATTCAA CCATCCTGGC AAGATTTACT GAAGCAGACT 
AAAGACAAAA TCTCCAACAA ACCAGAAAAT ATGAAGGAGT CTGAAAGAGC ACAAGATGAA 
AACATAAGGC GGAGTCCAAG ACGCTGTTCC AAGAATGCCT CAGCTAACAG AAAGGGCAAA 
GATCAAGACA TAAAACAGCC CAGAATGAAC AGCCCAGAGA AAGATAAGGA TGGACCACAA 
GATGTATTAA GTAGCCCAAG ACGTAGACCA AAGAAACCTT TGGAAGAACA AGACATAAGG 
AGGAGTCCAC GACACAGCAC ACATAAACCC CTAGCTGGAG CTGTAAATGC AGAACAAGAT 
GCAATAGTGA ATTCAAGGCA TCACCCAGAG CAAGATGAAA AACAAAATAC AAGCAGAACT 
ACAGAGCACA GCCCAGAGAA AGCTTTGGAT AAAGAAGACA AAGAGATGAT AAGAAGTCCA 
AGGCGCAGGA CTGAGAAAGT TATGAATGAA GACAAGAACA AAACAGGAAA GGGAAATCTT 
CATCCTGGCA ACAAAGAAAA GAGAAACATA CTACAAGACT TTGGCAATGT GGTCAGGAAT 
ATCCCTGATA CAATAACATT GGCTAGCCCC TCTCCTCCAA GGAGAAACTT CGCAGGTGTG 
GCGAGCAACA CAAGATCACA CAAGAGGGGA GAAGGGTGCA TCCTTGACTC AAGAAACGAT 
GAGGAAATCT TATCTTTGCC CGCAAGCCAA GACAGAAGTT TGGAGAACAG TATGGAAGAC 
TGTGGTGTGA TACTAGAGTC AGAGACTACT ACAGAGTTTG ACGGGAAGTT AGTGGAAGTG 
GTAGCTGATG TTCACAGGCT GGCAGAGTTC TCAGAGGGGA GCCAACAGTC TCAGAGACAG 
AACAAGTTAA GAAAAGCAGC ATCTGACATA CAGTCTCCTG GGCGGCATGC CAGTGGGAGG 
ACCCCAGCAT CACTCCGCAG CAGTGGGAGG GACAGCACCC CAGGGGAGGT GCCCCCCACC 
CCTCCCCCAC CCTCCACACC TCCTGTCTTA CCAGCTACTA TCAGCACCAG CGGTCAAAAA 
GCAGCAAAAG ACAGGAACAG GCTGAGCATA CAAAGGCGAA GTTCGCAACT TAGCTCGCCA 
GAGAATACGC CATCTATCTG TTCCAACTCT CAAGAGAACA CACCTTCTAC TCCAGGTGTC 
CAGAAGTCAC TACCAACTAG CACCAAGTCT CAGCCAAAGT CTAGTGCTAC GCCTCCTGAT 
GCAGTAGACA ATGAAAAGGA CACACCCAAG TCATGTCCTC CAGAGAGTGA AGTATCCACA 
GTGTGTAGTC AGGTATCCAC ACCAGAAGTT GACACAGACT CAGATGTGGT CCCAGACACT 
GAAGAAAATC CCACTAAGAA AGGAAAAAGG AAATCCTCAA ATTTGCAAAA GGCTTCAGAT 
TCAGAAGCTA CCCCCAGAAC GAAAGTGACA TCCCCAAGTA TGATGTTTCA GCAGTCTTTT 
CCAGATTCTG GAAATACAGA AGCTAGCGTC TCTCTCCTTG AGAATGTGAA ACCAAGGGGT 
GCAGAAACAG TTTCACAACA GAAGAGAAAA AGTCCTCGAC TCAGTAGAGG GGTATCGGAC 
AGTGGCAAAC AGGAGAGGAA ATGCAGCGAC GAACCGAAAG AGACAGTTAC ACAGAAACTG 
GAGCAAAGCG AAGCTGAACA GACCAACTCA AAACGTCATC TTAAAGAACA AAGAGGAAAG 
GAAGAAGTTA AAACTGACAA CTTGAAGCCT ACTGAGAAAG TGGTAGGAAA ACAAGCTAGC 
TTTGAAGGAC TAATTCAGCA ATCCAATGAA ATTGAACACT ACGATGTTAC AGATGATGAT 
AAGGAAGACC TTCAGATGGA AGACATTGAA GAGGAAGATG GACAAGAGGA AGAGGATGCT 
GTAGAAGATG AAGATGACAG TGAGGAGGAT GATCTTGTCC CTCCAACTCC TCCAGACACC 
TCCTTCACAC AACTGACCCA GAGAACCAAG ACTGTGAAGT CTAAAACTTG GACACCAGAA 
AGGGTTACAA GGGAAGACCT GAAAGAAAAC AACTTTCAAC CTGTCATATT AGAAGAAGAG 
GAAGATCGTG AGTGTAACTG GTCGGATGAT GACAATGATG AACTGCCTGA CCTTGACGAG 
GAACCAGAGC ATCAGGTGTC ATCCACAGGT CATTCCAACA CAGCAACTCC ACAGAATGGC 
GGAAGAAACA AGGCAGAGGA ACAACCAGAT GCGAAAGAAG GCTTCAAGGA AAACCAAAAA 
GACACTGACA TTAATACTCG TTCTCAGAGT AAGAGAAGGA AAGCAGCAAG TGGAGAAATT 
GAAGCGAAAG ACGAAGCAAC TGACGAATCA ATGCCCAAAA GGAGAAAGTC AGATATTGAC 
TCAGAGTGGA AAGAGGAAAG GATCCCTAAA GTTGATGTTG TCAACGTCAC AGCCGGCTTT 
GAGAATAATG AAGAGTCTGA CCTGAGCCAG GACAGCCACG TCCATGACCT GACCAATGCT 
TCATCCTTGA GTGAGCTGTA TGATACACAG AACCAAGACA AGTTAAAGAA CGATGTGCAG 
AACCTGGAGG GACAGATGGC CATGATACAG ATGCTCCTGG CCAAGGGGAA GGAGAGAGCG 
AGAGAGACTG GAAAAGAAGA GAACACCAAC AAAGATCCAA CCTCCATCGC TAAGCAGCAT 
CTCACAACAG TGTATGAAGA AAACATGCAG ATGTCAGAGG GTGATCATGA GGAAATTCTA 
AGTGGGAAGG ACTTGTATGG CACCCCTTCC TCACAAGCAT CTGCAAAGTG CACATACTCC 
ACAGCACAAG AGGAGACTCC TAGAAAACGG AGGCCCAGTA GCACCAGCAC ACCTCAATCT 
AATCACTCAC CTACCAAGGA GTACAACTTC CGCAAGAGAA TGTTGTCCCC TCAAAGATCG 
CAGGAGGCGT CTGCAAAGTC ACGAAAATCT CGGCAAAGTT CGCCAAGGTC GTCCCAACCT 
TGCTTGTCGC CACGCTCGCC CTCCCCGCCT CCGCCGCTTT CTCCGGAGAA GACGAGAACT 
CCGAGAGTGC TCCCGCCTGC AGTTCAGGCC GCAGCAAACA TTCTTGAGAA GTTCAGGAGC 
CCAGACCTTG CAACCAAAGG AAAAAGAAGG AATAGCGGTA ACAGTAGCCC ACTGGGAAAA 
GAAGACAGTA CAGCAAGAAG GCTAAGTCCT AGAACAAAGA AAGAAAGTCA AAGTCCTCCT 
GATAGGAAGA ACACTACCCC AAGAAGCAGC AGTTTTAAGA CGTCTGCTTC AAAGAGCCCT 
TCTAGTCCAA GGAGTAAGAA ATATGACATT GAGAAAAGAA TGAATCTTGA AGATGAAGTG 
GAAGACAGCA TGCCGGTTGA GAACTTGGTT TCGACTGCTG CCAGGAACAG GAGAAGTCTT 
AACAAGAGAA AGAGGAAGTC TTTACAGGAC ACGTTTGCAG ATGAAGAGGA TGAAACTATG 
AGGTGTGATG ATGCACCATC TCCTGGTGAC CACAAGATAC CAACTTCTGA AGAACCAGAG 
GAGATGTCAA CCAACTGGAA AGCATTTCAG CAAATGAAAT GTGATATTCT GGCCCAGAAA 
GGCACGTCTA CAAGTAATGG GCAAAACAAG GAGGCTCCCA AAAGACTGTC TCTGCCTTCA 
ACAATGAAAT CAGACAGATG TACTTCAGAC AAACGAAAAT CAGAGCCAGT CGTTTCTTCC 
TCTCAGTTTG ATAGAAACAG CATCACAAAC AACCCTTCTA CTCCACAACC GAGGAAGAAC 
CCACTAACGT TTAGCCAGCA GCCCTCACCA AGTGTGGGAA ACCTGTCTCA AACTGTTTCT 
AGAAGGAAGA AAGAGAGTCC ACGCCTGATT GGCAGTAGCA AATCACCCAT TGATCGCTTA 
CCCCATGGGA ACACTGAGAA GTTGACTTCC ACATCAAGTT GTAGTGCTGT GAAGAGTGTA 
GCAGGACCTC CCACAGTGGT GAAGACTGAG TGGCAGAATG ACAACCGTGC TCCCATGACA 
TTTGTGACAA CAGGACTGAG TAGAGAAGAG ACACGAGCAG TGCATGAACT GGCCAAAAAG 
ACAGGGTCTC AAGTCAAGCA GTCATTTGAC AACAGCACCA CTCATGTCAT CACCAGAACA 
GATGTGGACC TGTTCTGTTC CCGTACCCTG AAGTTCTTCT TGGGCATTGC AGGCAGAAAA 
TGGATTGTCT CCTTTCAGTG GATTGCGGCC TGCTTAGAGG AAGGAAAACA TGTGCCAGAG 
GATCCGTATG AAGTCCGAGG AGACCTGGTC CATGGTTGCC ATGGCGGCCC TCGACAAGCT 
CGCAGCAATA AGGGCAAGCT TCTCTTGTGT GATTATGAGA TCTGCATCTA TGGGAAGTTT 
ACTGCACTCA CCAGAGATGA CCTGCAGTTC ATGGTTGAGC TGTGTGGGGG CACTGTTGTT 
AAGGAACCTC ATATGTTTCC TCATGATAAG AAACCCTTAA TCGTGAGCCA GATAGATGCC 
AACCAGCTCA CAGAGGAAGA CTACAATGTG TTTTACCGAC GTTACGGAGT CTTGTCCGTG 
ACAAGGGACT GGATTCTGGA CAGCGTATCA AGTTACCAGA TCCAACCAAT CGAACGCTAC 
CTGATGTGTT CAACAAAGAA CCAGAACATC ATCGTCATCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002599481.1
CDS	1..7845
Translation	MDIFGMHITSQCLQEEGNGVPSTVQVRNRRNIRQAKKRKVEETPVYIEEGVSGDGHVTNGCHGDDDVTGNADPSAEDGRLLHVLGEASLGLFEQSGAKDIQESTTVLNSGGKVPTIEEHKEDQVGEKPLLTSHDHNDGSHADQEPHGDDQKSDTLGPTISEAEPLDISKDQEQMAVDLLTTEVEDEVPADIGKEVKAQEKVEFWLKHHNTTATEPGGTEDLDPYEFIGSQQDHAKGKKKSRKRGKKGRKPPAADNRNKKSPKSSSGPSKGKPTKTSAKKVEEVEDVEVDCEDLDQEEGEASAASMTDCSEASWKPGQSDPDWGKGRSSRRKSAETNADKESVSSKGRKRKQRNPADREDQMTVSSGSSSQLRIDRWVQRKEKLFVKSYSKPASSRREKTLLHKKKAAGLEEQWRRAKDMAGDFSAKVLHPIAEELPSKTKKKSKDNHTTSKASTKETCSATGLHPLQDLPSLGEREEVLNSEDDMCVYLTSNGELLNSPEKSDNPSDDDQDQEKQPTKEKSVALTEQEQKIAADLNELDEQTLSQLGMFSQQYLQKRKRRKDRKKRLEEVQNEDEDNIFAAIRALESESNLCNKEHQTQDETSRKEDNCTEVVDAGDVISAPVDEEVKKDNNSRKSRQARSLSRDRRGQGDVTEEKEARPRRTRSSSRKSAEDVLVDVGNAVSDRSSRQAGSSSKTKTNVRKNQNDKEDGETNGEEQSRPDCMKDECEHIPQDAGTSDKDKLAKKQKEETERQQTAKEEKTSEKELETRQEGSVNIEETAMKENATSPKALQMRKRHAAEKQTNKKALGNMGESLGVRQTRRNKKMPETVKEAEPPKHLPLVTTKDTVPQDPYQFQYSQESNRKKAGKKKGRRQRGKVKKLKRTKARKRELLLIQTGWIEREEAGEDEDSEEENGQENVEDQTVDELNVDESRDAQAHLDKQEERDQDTKVLGEEKDNTEVSVIPESNPNTEPISSSVKNKKHDVDLTACQPSCSLEETPGIYSTDIIPPSVEGTQIMSPQAGKKKRKTSLNKKKTEEDTSQRETGEDTKLSKSGKVSSPETENSEDIIQPSWQDLLKQTKDKISNKPENMKESERAQDENIRRSPRRCSKNASANRKGKDQDIKQPRMNSPEKDKDGPQDVLSSPRRRPKKPLEEQDIRRSPRHSTHKPLAGAVNAEQDAIVNSRHHPEQDEKQNTSRTTEHSPEKALDKEDKEMIRSPRRRTEKVMNEDKNKTGKGNLHPGNKEKRNILQDFGNVVRNIPDTITLASPSPPRRNFAGVASNTRSHKRGEGCILDSRNDEEILSLPASQDRSLENSMEDCGVILESETTTEFDGKLVEVVADVHRLAEFSEGSQQSQRQNKLRKAASDIQSPGRHASGRTPASLRSSGRDSTPGEVPPTPPPPSTPPVLPATISTSGQKAAKDRNRLSIQRRSSQLSSPENTPSICSNSQENTPSTPGVQKSLPTSTKSQPKSSATPPDAVDNEKDTPKSCPPESEVSTVCSQVSTPEVDTDSDVVPDTEENPTKKGKRKSSNLQKASDSEATPRTKVTSPSMMFQQSFPDSGNTEASVSLLENVKPRGAETVSQQKRKSPRLSRGVSDSGKQERKCSDEPKETVTQKLEQSEAEQTNSKRHLKEQRGKEEVKTDNLKPTEKVVGKQASFEGLIQQSNEIEHYDVTDDDKEDLQMEDIEEEDGQEEEDAVEDEDDSEEDDLVPPTPPDTSFTQLTQRTKTVKSKTWTPERVTREDLKENNFQPVILEEEEDRECNWSDDDNDELPDLDEEPEHQVSSTGHSNTATPQNGGRNKAEEQPDAKEGFKENQKDTDINTRSQSKRRKAASGEIEAKDEATDESMPKRRKSDIDSEWKEERIPKVDVVNVTAGFENNEESDLSQDSHVHDLTNASSLSELYDTQNQDKLKNDVQNLEGQMAMIQMLLAKGKERARETGKEENTNKDPTSIAKQHLTTVYEENMQMSEGDHEEILSGKDLYGTPSSQASAKCTYSTAQEETPRKRRPSSTSTPQSNHSPTKEYNFRKRMLSPQRSQEASAKSRKSRQSSPRSSQPCLSPRSPSPPPPLSPEKTRTPRVLPPAVQAAANILEKFRSPDLATKGKRRNSGNSSPLGKEDSTARRLSPRTKKESQSPPDRKNTTPRSSSFKTSASKSPSSPRSKKYDIEKRMNLEDEVEDSMPVENLVSTAARNRRSLNKRKRKSLQDTFADEEDETMRCDDAPSPGDHKIPTSEEPEEMSTNWKAFQQMKCDILAQKGTSTSNGQNKEAPKRLSLPSTMKSDRCTSDKRKSEPVVSSSQFDRNSITNNPSTPQPRKNPLTFSQQPSPSVGNLSQTVSRRKKESPRLIGSSKSPIDRLPHGNTEKLTSTSSCSAVKSVAGPPTVVKTEWQNDNRAPMTFVTTGLSREETRAVHELAKKTGSQVKQSFDNSTTHVITRTDVDLFCSRTLKFFLGIAGRKWIVSFQWIAACLEEGKHVPEDPYEVRGDLVHGCHGGPRQARSNKGKLLLCDYEICIYGKFTALTRDDLQFMVELCGGTVVKEPHMFPHDKKPLIVSQIDANQLTEEDYNVFYRRYGVLSVTRDWILDSVSSYQIQPIERYLMCSTKNQNIIVID

Target ORF information:

RefSeq Version	XM_002599435.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002599435.1

ORF Insert Sequence:

ATGGACATTT TCGGCATGCA CATCACCTCC CAATGTTTGC AGGAAGAAGG AAATGGGGTG 
CCATCAACTG TACAGGTCCG GAACAGACGA AACATCAGAC AAGCCAAGAA GAGGAAAGTT 
GAGGAAACTC CTGTTTACAT TGAGGAAGGT GTATCAGGTG ATGGTCATGT GACCAATGGT 
TGCCATGGTG ATGATGATGT CACAGGAAAT GCCGACCCCT CGGCAGAAGA TGGCAGGCTC 
CTGCACGTGC TTGGGGAGGC CTCGCTTGGA CTGTTTGAGC AAAGTGGAGC AAAAGACATT 
CAGGAATCTA CAACAGTTCT TAATTCTGGA GGAAAAGTTC CAACAATAGA AGAACATAAA 
GAAGACCAGG TTGGTGAGAA GCCTCTACTG ACAAGCCATG ATCACAATGA TGGGAGTCAT 
GCTGACCAGG AACCCCATGG AGATGACCAG AAGTCGGACA CACTCGGCCC CACCATCAGC 
GAGGCAGAAC CATTGGACAT TAGCAAGGAC CAGGAACAGA TGGCCGTCGA CCTTCTGACA 
ACAGAAGTTG AAGATGAAGT TCCCGCAGAC ATAGGGAAGG AGGTAAAGGC ACAGGAGAAG 
GTAGAGTTCT GGCTGAAACA CCACAACACA ACTGCCACAG AACCAGGGGG CACAGAGGAC 
CTGGATCCAT ATGAGTTCAT AGGCAGCCAG CAGGATCATG CAAAAGGGAA GAAGAAGTCT 
AGGAAAAGGG GGAAGAAAGG AAGAAAGCCT CCTGCAGCTG ACAACAGGAA CAAGAAGTCT 
CCTAAGTCTT CTTCTGGTCC TTCTAAAGGC AAACCTACGA AGACATCAGC TAAGAAGGTT 
GAAGAAGTGG AAGATGTAGA GGTAGACTGT GAAGACTTGG ATCAGGAAGA AGGGGAGGCC 
TCAGCTGCTA GCATGACTGA CTGTTCGGAG GCATCATGGA AGCCTGGCCA GTCTGACCCG 
GATTGGGGGA AGGGAAGAAG CTCGCGACGG AAGTCTGCAG AGACAAACGC TGACAAAGAA 
TCTGTATCCA GCAAAGGTCG TAAGAGGAAG CAAAGAAATC CTGCCGACAG GGAGGACCAG 
ATGACAGTGT CTTCAGGAAG CAGCAGTCAG CTGCGCATCG ACCGATGGGT GCAGAGAAAA 
GAGAAACTGT TTGTCAAGAG CTACAGCAAA CCTGCTTCGT CCCGGCGCGA GAAAACTTTA 
CTCCATAAGA AGAAGGCTGC AGGTCTTGAG GAACAGTGGC GGAGAGCCAA GGACATGGCC 
GGAGACTTCT CCGCCAAAGT CTTGCACCCT ATCGCCGAAG AACTCCCCAG CAAGACTAAG 
AAGAAATCTA AAGACAACCA CACAACATCA AAAGCTTCTA CAAAGGAAAC CTGTAGTGCA 
ACAGGTCTAC ATCCTTTGCA GGATTTGCCA AGTCTAGGAG AGAGAGAAGA AGTACTCAAT 
AGTGAAGATG ACATGTGTGT TTACCTCACA AGCAATGGAG AACTCCTCAA CAGCCCTGAA 
AAGAGCGACA ACCCAAGTGA TGATGATCAA GATCAGGAAA AACAGCCAAC CAAGGAGAAG 
TCTGTAGCAT TGACGGAACA AGAACAGAAG ATCGCAGCAG ATCTCAATGA GCTCGATGAA 
CAGACACTCA GTCAGCTAGG CATGTTCTCA CAGCAGTACC TTCAGAAGAG GAAGAGAAGG 
AAGGATAGGA AGAAGAGACT TGAAGAGGTG CAAAATGAGG ATGAGGACAA CATCTTCGCA 
GCAATCCGGG CTTTAGAAAG TGAGAGCAAT CTTTGCAACA AGGAACATCA AACCCAAGAT 
GAAACATCAA GAAAAGAAGA TAACTGCACA GAAGTAGTGG ATGCAGGAGA TGTAATTTCA 
GCACCAGTGG ATGAAGAAGT GAAGAAGGAT AACAATTCCA GAAAGTCCCG ACAGGCAAGG 
TCTTTGTCCC GAGATCGCAG AGGTCAAGGT GATGTCACAG AGGAAAAAGA AGCAAGGCCA 
AGGCGGACAA GATCTTCTTC AAGGAAGAGT GCAGAAGATG TCCTTGTAGA TGTTGGGAAT 
GCTGTGTCCG ACAGAAGCAG TAGACAAGCA GGATCTTCAT CTAAGACTAA GACTAATGTA 
AGGAAGAATC AGAATGACAA GGAAGATGGT GAAACAAATG GTGAAGAACA GTCAAGACCA 
GATTGCATGA AGGATGAGTG TGAACATATC CCCCAAGATG CAGGAACATC TGACAAAGAT 
AAGCTTGCTA AGAAGCAGAA GGAAGAAACA GAAAGACAGC AAACTGCAAA AGAAGAAAAG 
ACATCTGAAA AGGAATTGGA AACAAGGCAA GAAGGTTCTG TCAACATTGA AGAAACTGCC 
ATGAAGGAAA ATGCAACGTC ACCAAAGGCT TTGCAGATGA GAAAACGTCA CGCTGCAGAA 
AAACAAACTA ACAAGAAAGC TCTTGGCAAC ATGGGAGAAT CGCTTGGCGT ACGACAGACA 
AGACGCAACA AGAAGATGCC TGAGACCGTG AAGGAAGCTG AACCTCCGAA GCATCTCCCT 
CTTGTGACAA CAAAAGACAC AGTTCCTCAG GATCCTTACC AGTTTCAGTA CAGCCAGGAA 
AGCAACAGAA AGAAAGCAGG AAAGAAGAAG GGAAGGAGAC AACGGGGTAA AGTGAAGAAG 
CTGAAAAGGA CAAAAGCAAG GAAAAGGGAG TTGCTGTTGA TCCAAACAGG ATGGATAGAA 
AGAGAAGAGG CTGGAGAAGA TGAAGATAGT GAAGAGGAAA ATGGTCAGGA GAATGTGGAA 
GACCAAACGG TTGACGAGTT AAATGTGGAT GAATCTAGGG ATGCCCAAGC ACACTTAGAC 
AAGCAAGAAG AAAGGGACCA GGATACAAAG GTCCTTGGTG AAGAGAAGGA TAATACAGAA 
GTATCAGTTA TTCCAGAGTC AAACCCCAAC ACAGAGCCCA TCTCAAGCAG TGTGAAGAAC 
AAAAAGCATG ATGTTGACCT TACTGCCTGT CAACCAAGTT GTTCATTGGA GGAAACTCCA 
GGCATCTACT CCACTGACAT TATCCCTCCA TCTGTAGAGG GCACTCAGAT AATGTCTCCT 
CAAGCTGGCA AAAAGAAGAG GAAGACCTCC TTGAATAAGA AGAAGACAGA GGAAGACACA 
AGTCAGAGGG AGACAGGGGA AGACACAAAA CTGTCCAAGT CTGGAAAAGT TTCATCGCCA 
GAGACGGAAA ACTCTGAGGA CATCATTCAA CCATCCTGGC AAGATTTACT GAAGCAGACT 
AAAGACAAAA TCTCCAACAA ACCAGAAAAT ATGAAGGAGT CTGAAAGAGC ACAAGATGAA 
AACATAAGGC GGAGTCCAAG ACGCTGTTCC AAGAATGCCT CAGCTAACAG AAAGGGCAAA 
GATCAAGACA TAAAACAGCC CAGAATGAAC AGCCCAGAGA AAGATAAGGA TGGACCACAA 
GATGTATTAA GTAGCCCAAG ACGTAGACCA AAGAAACCTT TGGAAGAACA AGACATAAGG 
AGGAGTCCAC GACACAGCAC ACATAAACCC CTAGCTGGAG CTGTAAATGC AGAACAAGAT 
GCAATAGTGA ATTCAAGGCA TCACCCAGAG CAAGATGAAA AACAAAATAC AAGCAGAACT 
ACAGAGCACA GCCCAGAGAA AGCTTTGGAT AAAGAAGACA AAGAGATGAT AAGAAGTCCA 
AGGCGCAGGA CTGAGAAAGT TATGAATGAA GACAAGAACA AAACAGGAAA GGGAAATCTT 
CATCCTGGCA ACAAAGAAAA GAGAAACATA CTACAAGACT TTGGCAATGT GGTCAGGAAT 
ATCCCTGATA CAATAACATT GGCTAGCCCC TCTCCTCCAA GGAGAAACTT CGCAGGTGTG 
GCGAGCAACA CAAGATCACA CAAGAGGGGA GAAGGGTGCA TCCTTGACTC AAGAAACGAT 
GAGGAAATCT TATCTTTGCC CGCAAGCCAA GACAGAAGTT TGGAGAACAG TATGGAAGAC 
TGTGGTGTGA TACTAGAGTC AGAGACTACT ACAGAGTTTG ACGGGAAGTT AGTGGAAGTG 
GTAGCTGATG TTCACAGGCT GGCAGAGTTC TCAGAGGGGA GCCAACAGTC TCAGAGACAG 
AACAAGTTAA GAAAAGCAGC ATCTGACATA CAGTCTCCTG GGCGGCATGC CAGTGGGAGG 
ACCCCAGCAT CACTCCGCAG CAGTGGGAGG GACAGCACCC CAGGGGAGGT GCCCCCCACC 
CCTCCCCCAC CCTCCACACC TCCTGTCTTA CCAGCTACTA TCAGCACCAG CGGTCAAAAA 
GCAGCAAAAG ACAGGAACAG GCTGAGCATA CAAAGGCGAA GTTCGCAACT TAGCTCGCCA 
GAGAATACGC CATCTATCTG TTCCAACTCT CAAGAGAACA CACCTTCTAC TCCAGGTGTC 
CAGAAGTCAC TACCAACTAG CACCAAGTCT CAGCCAAAGT CTAGTGCTAC GCCTCCTGAT 
GCAGTAGACA ATGAAAAGGA CACACCCAAG TCATGTCCTC CAGAGAGTGA AGTATCCACA 
GTGTGTAGTC AGGTATCCAC ACCAGAAGTT GACACAGACT CAGATGTGGT CCCAGACACT 
GAAGAAAATC CCACTAAGAA AGGAAAAAGG AAATCCTCAA ATTTGCAAAA GGCTTCAGAT 
TCAGAAGCTA CCCCCAGAAC GAAAGTGACA TCCCCAAGTA TGATGTTTCA GCAGTCTTTT 
CCAGATTCTG GAAATACAGA AGCTAGCGTC TCTCTCCTTG AGAATGTGAA ACCAAGGGGT 
GCAGAAACAG TTTCACAACA GAAGAGAAAA AGTCCTCGAC TCAGTAGAGG GGTATCGGAC 
AGTGGCAAAC AGGAGAGGAA ATGCAGCGAC GAACCGAAAG AGACAGTTAC ACAGAAACTG 
GAGCAAAGCG AAGCTGAACA GACCAACTCA AAACGTCATC TTAAAGAACA AAGAGGAAAG 
GAAGAAGTTA AAACTGACAA CTTGAAGCCT ACTGAGAAAG TGGTAGGAAA ACAAGCTAGC 
TTTGAAGGAC TAATTCAGCA ATCCAATGAA ATTGAACACT ACGATGTTAC AGATGATGAT 
AAGGAAGACC TTCAGATGGA AGACATTGAA GAGGAAGATG GACAAGAGGA AGAGGATGCT 
GTAGAAGATG AAGATGACAG TGAGGAGGAT GATCTTGTCC CTCCAACTCC TCCAGACACC 
TCCTTCACAC AACTGACCCA GAGAACCAAG ACTGTGAAGT CTAAAACTTG GACACCAGAA 
AGGGTTACAA GGGAAGACCT GAAAGAAAAC AACTTTCAAC CTGTCATATT AGAAGAAGAG 
GAAGATCGTG AGTGTAACTG GTCGGATGAT GACAATGATG AACTGCCTGA CCTTGACGAG 
GAACCAGAGC ATCAGGTGTC ATCCACAGGT CATTCCAACA CAGCAACTCC ACAGAATGGC 
GGAAGAAACA AGGCAGAGGA ACAACCAGAT GCGAAAGAAG GCTTCAAGGA AAACCAAAAA 
GACACTGACA TTAATACTCG TTCTCAGAGT AAGAGAAGGA AAGCAGCAAG TGGAGAAATT 
GAAGCGAAAG ACGAAGCAAC TGACGAATCA ATGCCCAAAA GGAGAAAGTC AGATATTGAC 
TCAGAGTGGA AAGAGGAAAG GATCCCTAAA GTTGATGTTG TCAACGTCAC AGCCGGCTTT 
GAGAATAATG AAGAGTCTGA CCTGAGCCAG GACAGCCACG TCCATGACCT GACCAATGCT 
TCATCCTTGA GTGAGCTGTA TGATACACAG AACCAAGACA AGTTAAAGAA CGATGTGCAG 
AACCTGGAGG GACAGATGGC CATGATACAG ATGCTCCTGG CCAAGGGGAA GGAGAGAGCG 
AGAGAGACTG GAAAAGAAGA GAACACCAAC AAAGATCCAA CCTCCATCGC TAAGCAGCAT 
CTCACAACAG TGTATGAAGA AAACATGCAG ATGTCAGAGG GTGATCATGA GGAAATTCTA 
AGTGGGAAGG ACTTGTATGG CACCCCTTCC TCACAAGCAT CTGCAAAGTG CACATACTCC 
ACAGCACAAG AGGAGACTCC TAGAAAACGG AGGCCCAGTA GCACCAGCAC ACCTCAATCT 
AATCACTCAC CTACCAAGGA GTACAACTTC CGCAAGAGAA TGTTGTCCCC TCAAAGATCG 
CAGGAGGCGT CTGCAAAGTC ACGAAAATCT CGGCAAAGTT CGCCAAGGTC GTCCCAACCT 
TGCTTGTCGC CACGCTCGCC CTCCCCGCCT CCGCCGCTTT CTCCGGAGAA GACGAGAACT 
CCGAGAGTGC TCCCGCCTGC AGTTCAGGCC GCAGCAAACA TTCTTGAGAA GTTCAGGAGC 
CCAGACCTTG CAACCAAAGG AAAAAGAAGG AATAGCGGTA ACAGTAGCCC ACTGGGAAAA 
GAAGACAGTA CAGCAAGAAG GCTAAGTCCT AGAACAAAGA AAGAAAGTCA AAGTCCTCCT 
GATAGGAAGA ACACTACCCC AAGAAGCAGC AGTTTTAAGA CGTCTGCTTC AAAGAGCCCT 
TCTAGTCCAA GGAGTAAGAA ATATGACATT GAGAAAAGAA TGAATCTTGA AGATGAAGTG 
GAAGACAGCA TGCCGGTTGA GAACTTGGTT TCGACTGCTG CCAGGAACAG GAGAAGTCTT 
AACAAGAGAA AGAGGAAGTC TTTACAGGAC ACGTTTGCAG ATGAAGAGGA TGAAACTATG 
AGGTGTGATG ATGCACCATC TCCTGGTGAC CACAAGATAC CAACTTCTGA AGAACCAGAG 
GAGATGTCAA CCAACTGGAA AGCATTTCAG CAAATGAAAT GTGATATTCT GGCCCAGAAA 
GGCACGTCTA CAAGTAATGG GCAAAACAAG GAGGCTCCCA AAAGACTGTC TCTGCCTTCA 
ACAATGAAAT CAGACAGATG TACTTCAGAC AAACGAAAAT CAGAGCCAGT CGTTTCTTCC 
TCTCAGTTTG ATAGAAACAG CATCACAAAC AACCCTTCTA CTCCACAACC GAGGAAGAAC 
CCACTAACGT TTAGCCAGCA GCCCTCACCA AGTGTGGGAA ACCTGTCTCA AACTGTTTCT 
AGAAGGAAGA AAGAGAGTCC ACGCCTGATT GGCAGTAGCA AATCACCCAT TGATCGCTTA 
CCCCATGGGA ACACTGAGAA GTTGACTTCC ACATCAAGTT GTAGTGCTGT GAAGAGTGTA 
GCAGGACCTC CCACAGTGGT GAAGACTGAG TGGCAGAATG ACAACCGTGC TCCCATGACA 
TTTGTGACAA CAGGACTGAG TAGAGAAGAG ACACGAGCAG TGCATGAACT GGCCAAAAAG 
ACAGGGTCTC AAGTCAAGCA GTCATTTGAC AACAGCACCA CTCATGTCAT CACCAGAACA 
GATGTGGACC TGTTCTGTTC CCGTACCCTG AAGTTCTTCT TGGGCATTGC AGGCAGAAAA 
TGGATTGTCT CCTTTCAGTG GATTGCGGCC TGCTTAGAGG AAGGAAAACA TGTGCCAGAG 
GATCCGTATG AAGTCCGAGG AGACCTGGTC CATGGTTGCC ATGGCGGCCC TCGACAAGCT 
CGCAGCAATA AGGGCAAGCT TCTCTTGTGT GATTATGAGA TCTGCATCTA TGGGAAGTTT 
ACTGCACTCA CCAGAGATGA CCTGCAGTTC ATGGTTGAGC TGTGTGGGGG CACTGTTGTT 
AAGGAACCTC ATATGTTTCC TCATGATAAG AAACCCTTAA TCGTGAGCCA GATAGATGCC 
AACCAGCTCA CAGAGGAAGA CTACAATGTG TTTTACCGAC GTTACGGAGT CTTGTCCGTG 
ACAAGGGACT GGATTCTGGA CAGCGTATCA AGTTACCAGA TCCAACCAAT CGAACGCTAC 
CTGATGTGTT CAACAAAGAA CCAGAACATC ATCGTCATCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_122749 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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