BRAFLDRAFT_70263 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_70263
Entrez Gene ID	7224557
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002599746.1	XP_002599792.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_70263 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_70263 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb09115	XM_002599746.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_70263 ( XM_002599746.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002599792.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb09115
Clone ID Related Accession (Same CDS sequence)	XM_002599746.1
Accession Version	XM_002599746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101465). COMPLETENESS: incomplete on both ends.

ATGGGGGAAC CGTCGAGCTT TCTCCACATC GGCGACATCG TGTCCCTGTT CGCCGAGGGC 
TCTGTCAGCG GTTTCATATC AACTCTTGGT CTTGTTGATG ACCGATGCGT GGTGCAGCCA 
GACGCGGGCG ACCTGTCCAA CCCGCCCAAG AAGTTCCGAG ACTGCCTGTT CCGGATCTGT 
CCTATGAACC GGTATTCTGC CCAGAAGCAG TTCTGGAAGG CAGCCAAACC CAACCCCTCC 
AACCCTGCTG ATGCTGTCCT GCTCAAAAAG CTACAGCATG CAGCAGACCT GGAGAAGAAA 
CAAAATGAGG CTGAGAACAA GAAGCTACTT GGCAGTGTCA TTCAGTATGG AGGAGTCATA 
CAGAGCAACA AGTTCCTGAC AGTGAACAAG CGCCTCCCGG CACATCTGGA GAAAAATGCA 
ATGAGAGTCA CCCTTGATCC ATCCGGAAAT GAAGGGTCAT GGTTTTACAT CCAGCCCTTT 
TACAAGCTGA GATCACCAGG GGATAATGTT GTTGTTGGTG ACAAGGTGAC CCTGATGCCA 
GTCAATGCTG GACAGCCTCT CCATGCTAGT AACTATGAGC TGGTGGACAA CCCTGGTTGT 
AAAGAGGTGA ACTCTGTAAA CTCTAATACG AGTTGGAAGG TATCGCTCTT CATGATCTAC 
ACAGAGAACA TTGATGATGT GCTGAAGGGA GGGGACGTGG TACGGCTATT CCATGCTGAG 
CAGGAGAAGT TCCTGACAGG AGATGAGTAC AAGAGGAAGG AGTGTGTGTT CCTGAGGGTC 
ACCGCCAGAC AATCAGCAAC CTCAGCTACA AGTTCCAAAG CCCTGTGGGA AGTTGAGGTG 
GTTCATCACG ACCCCTGTAA GGGTGGCGCC GGTCACTGGA ACAGCCTCTT CAGGTTCAAA 
CACCTGGCCT CTGGGACCTA CCTGGCAGCA GAGATTGACC ATGACCCGAC CCCTGACACC 
CAGCGGCAGA AGCTGGCGGG GCCGCAGGGC GGGATGGTTT ACCAGCTGAT GCCCATACCC 
CACGGGCACG ACATCGCCTC CATCTTTGAA CTGGATCCCA CCACCATGCA GAGAGCAGAC 
TCAATGGTTC CGAGGAGCTC CTACGTGCGT CTGCGACACC TGTGCACGAA CACCTGGGTA 
CACAGCACAG CCATACCTAT AGACAAGGAG GAGGACAAAC CTGTCATGCT CAAGGTTGGT 
GCTGCTCCAA TCCGGGAGGA TAAGGAAGCC TTTGCCATTG TTCCTGTCCC TGCCAACGAG 
GTCAGAGACC TGGACTTTGC CAATGACGCC TGCAAGGTGA TGCAGGACAT AGCTAGCAAA 
CTAGAGAAAA GCAGCATCAC TCAGAACGAG AGAAGTTCTG TTTCCCAACT GCCAACTCGG 
CCGGCCATCA ACAATGTGCT GTCAAAGAGC GTCCGGAGGT CTGGCTGTTT TCTGGACTTG 
GTCCTGCAAC AACAGCCTCC CCTGCAGGGA GGACTGCTGA CGGACCTGGT GTATTTCCTG 
GCAGACAAGC CCAACAACGG AGGGGACCCC CTGGACGTGC AGATCACCAA CCCAAACAGG 
GAGAGGCAGA AGCTCATGAG AGATCAGAAC ATTCTTAAAC AGATCTTCAA AGTTCTCCAG 
GCGCCCTTTG AGGACAAGGG TGATGGTCCT ATGCTGCGGA TGGAGGAGCT GTCTGACCAG 
AGACACGCCC CCTTCAGGCA CATCTGCAGG CTGTGCTACA GGGTGCTCAG GCTGTCACAG 
CAGGACTACA GGAAGAACCA GGAGTACATA GCAAAGCAGT TTGGTTTTAT GCAAAAGCAG 
ATCGGCTATG ATGTGTTGGC TGAAGACACC ATCACAGCTC TTCTGCACAA TAACAGAAAA 
CTGCTGGAAA AGCATATCAC CGCTGCTGAG ATAGAGACCT TCGTTAGCCT TGTTAGGCAG 
AAGAAGGAAT GCAGATTTCT GGAATACCTG TCAGACCTGT GTGTGTCCAA CAACCAGGCC 
ATCCCTGTCA CCCAGGAGCT GATCTGTAAG TCTGTCCTCA AGCCAGCCAA CAGTGACATC 
CTCACGGAAA CTAGAATTGT GAAGACAGAA GTGGAGGAGG TGATAGAGGA TGAGGAGGGT 
GAGGATGGAG AGCCCATCAC CCAGATTGTG GAGGAGGAGG AGGTGGTGCT GTTCTGGGAG 
AAGGGACACA AACATAAGGC CATCCGGGAG CTGGCCGCTG ATGCCGAGGA GTCTAAGGAA 
GACAAGCAAG TTATGGACTA CTACAGATAC CAGCTGGACC TGTTCTCCCA GATGTGCCTG 
GACAGACAGT ACCTCGCCAT CAACATCCTC TCCACACAAC TGGACATCAA CCTTATATTG 
CAGTGCATGT CGGATGAGTC GTTGCCGTAC GACCTTCGCG CTTCGTTCTG CCGGCTGATG 
CTGCACATGC ATGTGGACAG GGACCCCCAG GAGCAGGTCA CCCCTGTCAA GTATGCACGC 
CTCTGGTCCG AGATCCCCTC CAAAATCACG ATAGAGGACT ATGACTCCAC CAGGGACAAG 
AGTAAGGCGG ACATGAGACC CAGGTTTGAG GACACCATGC GGTTTGTAGA GCGCTACCTC 
CGGCATAACC TTGCCGACAA TGCACGAGGA TGGCAGTTCG CTGATCGGAA CCAGAACAAA 
CTAACATTTG AGGTGGTGAA CCTGTCCCGA CACCTGGTGT ACTTCGGGTT CTACAGCTTC 
AGCGACCTGC TGAGACTGAC CCAGACCCTC CTGTCCATCC TGGACATCAC CAACTCCACC 
ACACCCGTCC TGGCGCTGGG CAAGATGGAC AAGGTGTCCG AGCTAGTGGG TAAGACGGGA 
GTGTTCAAGT CCCTGCACGA CGTGAGCGCT GTGATGACCA CCATGGTGCT GGGGGGAGGT 
GGCATGTCCG GGGGGAAGGC TCTCAAGGCT GGGGACGGAG AGATGGACAC CATCGTCATG 
GATACCAAAC TGAAGATCAT TGAAATTCTG CAGTTTATCC TGAACGTCCG TCTGGACTAC 
CGCATCTCCT GTCTCCTGTC CATCTTCAAG AACGACTTTG ATGAGAGTAA CAGCAGCCAG 
CGTGGTGATA CCCTCCCTGG GCTGGGGACT AATGCACAGG ACAACTCTGT GGTAGATTTA 
GAGAAGATTG CACAACAAGC AGAAAGCATC TTTGATGGCA GTGATGAGTC AGCAGATCTG 
GACCTGGATG GTGCGGGAGG GCGGACGTTC CTGAGAGTCC TGCTGCACCT GACCATGCAT 
GACTACCCAC CCCTGGTGTC AGGGGCGCTG CAGCTGCTCT TCAGACACTT CAGCCAAAGG 
CAGGAGGTGC TGCAGGCCTT CAAACAGGTA CAACTGCTGG TCTCCTCCAG CGATGTGGAG 
AACTACAAGC AGATCAAAGC TGACCTGGAC GAGCTCAGAC TGCTTGTGGA GAAGTCCGAA 
CTCTGGGTCT ACAAGAGCAA GAGTGAAGGG GGCAAAAAGG AGAAGAAGGA GTCCAGTACA 
GGGGATGGGG ACGGAGACAA GAAGAAGAAA TCTGAGGGTG GGGCTGACAA AAAGGAGGGG 
AAATCGTCCT TGACATCATC CCAAGGTCCA GAAGGTTCTG CTATTGACCT TGATATTGGA 
CCTTCCATGG ATCAAAATAG CGTCAAGAAT TACAAGACCA TCCAGCAGGT TCTGATTCGC 
CTGAGCCAGC TTTGCGTCCA CCAGTCCAGC GGGAAGAACC GTAAACACGA GCAGCGTCTG 
CTGAGGAACA TGGGAGCCCA CAGTGTTGTA CTGGAACTCC TCCAGATCCC CTTTGATAAG 
AAGGAAGACA CCAGGATGAA CGAGATCATG CGGTTAGCCC ATGAGTTCCT TCAGAAGTTC 
TGCCAAGGAA ATCCAGCCAA TCAGGCACTG CTACACAAAC ATGTGGACCT GTTCCTGACT 
CCTGGGCTCT TGGAGGCAGA AACTATGAGA CACATATTCA TGGACAACAG CATGCTGTGC 
AATGAAGTGA CAGAGAGAGT GGTGCAGCAC TTTGTTCACT GCATCGAGAC CCACGGTCGC 
CATGTGCAGT ATCTGAAGTT CCTACAGACC ATTGTGAAAG CAGAGGGACA ATACATCAGG 
AAATGTCAAG ACATGGTCAT GGCAGAGTTG GTGAATGTAG GAGAAGACGT CCTGCTGTTC 
TATAACGACA GGTCGTCCTT CAACACCCTG ATCGAGATGA TGCGGTCGGA GCGAGAGAAG 
AGACAGCTGA GCAGCCCTCT GATGTACCAC ATCAACCTGG TGCAGCTGCT GGGGCTGTGC 
ACCGAGGGCA AGAACGTGTA CACAGAGATC AAGTGCCACT CCCTGCTCCC TCTGGATGAT 
ATCAGCAGGG TGGTGGTGCA TCCGGACTGT CTACCTGAGG TTAAAGATGC TTACATCAAC 
TTCCTGAACC ACTGCTATGT GGACACTGAG GTGGAGATGA AGGAGATCTA TACCAGCAAC 
CACATGTGGA CTTTGTTTGA GAACTTCCTT GTCGACATGG CCACGGTTTG CAAAGCCACA 
GATCGTAACC ACGCCGACCC AGTTCTAGAG CATTACGTCA CCAACACCAT CATGAACATC 
ATCACCATGT TCTTCAGCTC CCCCTTCTCA GACCAGAGCA CCACAGTTCA GACCCGACAG 
CCAATCTTCG TCCAACTGCT GTCTGGAGCG TATCGTGTGT CGCAGTGTAC ATGGCTGAAT 
ACACAGCAGC GCTGCATGGT GGAGACATGC ATCAAAACCC TGACAGAAAT AGCCAAGAAC 
CGTGGCATAG CCATCCCTGT GGACTTGGAC AGCCAGGTGA ACACACTGTT CTCCAAGTCC 
CAACTCCTCA TGGCCACATC CCGTAAGTGG CTGAGTACGC GTGGCTCGTC CAGACGAGAC 
TCCATCATGG CCATTTCACG GGACACCAGG AGCATTATTG AAGGGTTGCA GGTCAGTGTT 
TTTTCTCATC TTTCCTTTCC TCTCACCTCC CTGAGTCAGC AGTGGCAGGA CTTGGTGCCT 
CCTCCCATGG CTGCCAACAG GCGGATTGTG GAGCTAACAA CCAAAAACCT GCCACAGGAC 
ATTGTGTCCT TGCTGGAGGA CCAGCTGCGG CCGCTGGTCC AGGCGGAGCT GTCCGTGCTG 
GTGGACGTGC TGCACAGACC CGAGCTGCTG TTCCCACCGG GATCGGAGGC CAGACAGAAC 
TGTGAATCTG GCGGATTCAT CTCCAAACTT ATCAAACACA CGGAGAGGCT GCTAGAAGAG 
AAGGAGGAGA AACTGTGCAT CAAGGTGCTG CAAACTCTCA AGGAAATGAT GACCACAGAC 
ATTGACTATG GAGAAAAGGG AGACAAGTTA CGCAGCAGCT TGTTGACACG GTACTACGGA 
AAGCAGCACC CGCGAGCAGA CCGCAACCGC AAGGAGAGCG GATCTCCGAA TGCGCAGCCG 
CCCCAGCGCC CGTCTGTGGC CAACCCCGCC CCTGGGGCAC AGATGGTGGG GAGGGCAGAG 
ATGTCCCTGG CGGAGGTGCA GTGCCACTTG GACAGAGAGG GGGCATCGCT TCTGGTCACA 
GACTTGGTCA TCAATAGCAC CAGCAACAGG GTGTTTGAGG AAGCAGTGGA GCTTGGGATA 
GCATTACTGG AGGGTGGCAA CAGCACCATA CAGAAATCTT TCCTGAACCA CCTGCGGGAT 
GAGGGGAAGG CAGAGAAGTT CTTTAAGGTT TTCTATGACC GGATGAGGGA GTCTCAGCAG 
GAGATCAAGG CCACAGTCAC TGTTAGCACC GGCGACAGTC TGTCAGGCAA CAAGGACAAC 
AAAGATGTGG CAGTGAAGGA CACACCAGCA AAAATGAAGA AAGGTGCAGG TGGAGGCAGC 
CCAGGGACGG CCATGTCAGA CGATGTGAGG GACCAGCTAG CTGATGCAGC GCTGCAGACC 
AGCAAAGCCT TCGGAGCCAT GAGGCAGGGA CCACAGGGGG AGGACGCTGC CAACCCTCTC 
TCAGGATTTG ACAAGGAGAC AGAGAAGAAG AACGAGGAGC GAGGCATGAG CCCTGAGATC 
GCCATCATGA AGCCCATCCT GAGGTTCCTG CAGCTGCTGT GTGAGAACCA CAACAGGGAC 
CTGCAGAATT ACCTGCGGAA CCAGAACAAC AAGACCAACT ACAACCTTGT GTGTGAGACT 
CTGCAGTTCC TGGACTGCAT CTGTGGCAGC ACCACCGGTG GGCTGGGCCT GCTGGGGCTG 
TACATCAACG AGAAGAACGT CGATCTCATC AACCAGACAC TGGCCAGTCT GACCGAGTAC 
TGCCAGGGAC CCTGCCACGA GAATCAGAAC TCTATTGCCT GCCATGAGTG TAATGGAATT 
GACATCATCA CAGCTTTGAT TCTTAATGAC ATCAACCCTC TGGGTACACA CCGTATGGAC 
TTGGTGTTAG AGCTCAAGAA CAATGCCAGT AAGCTGCTAC TTGCTATCAT GGAAAGCCGA 
CATGACAGTG AGAATGCTGA AAGGATCTTG TACAACGTCA GTCCAAAGCA GTTGGTTGAT 
GTGATTAAGA GGGCGTACAT GCAGGAGGAG ATGAAGGAGG ATGAGGAGGA TGAGGAAAGT 
GAAGAGGATG AGGAAGAGGA CAAAGTCTCA CCGAAGGAAG TTGGACACAA CATCTATATC 
TTGGCACATC AGCTCTCGCA GCACAACAAG GAGCTGGCAG CCCTGCTGAA ACCCAGTAGT 
GCTGCAGACT ACGGCAACAC AGCCCTGGAG TACTATGCCA AACACACTGC ACAAATTGAG 
ATTGTGCGTC AGGACAGGAC CATGGAGAGG ATTGTGTTCC CTGTGCCCAG TATCTGTGAG 
TACCTGACGG AGGAGACAAA GTCACGTGTG TACCACACCG CCGAGAGGGA TGAGCAGGGC 
AGCAAGGTGT CCGACTTCTT CGAGAGATGT GACGACATGT TCAATGAGAT GAAGTGGCAG 
AAAAAACTCA GAGCCAACCC CACCCTGTAC TGGATCAGTG CTCACATGTC CCTGTGGAGC 
GCCATCTCCT TCAACCTGGC TGTGCTCATC AACCTCCTCA TCGCCTTCTT CTTCCCCTTC 
TCAGGAGGAA CTGGAGAACT TGACCCTCGG CTGTCTGGTC TGGTGTGGAC GGCCATGCTG 
GTTTCCCTGG CAACAGTCAT CACCCTACCC AAAGCCTCCG GGATACGAAC ACTGGTGGCC 
TCCACTATAC TCAGGCTGAT ATTCAGTGTG GGGCTGGAGC CAACCCTGTG GTTGATCGGC 
TGTGCAAATG TGATAAACAA GGCAGTGTTC CTGGTGAGCC TGCTGGGGAA CAGAGGGACC 
TTCACCAAGA ACCTGCAGGC CATCATGGGG GATGTGGAGC TGTGGTACTA CGTGGCGTAC 
CTCATCCTCT GTGTGCTGGG CCTCACCATG CACGAGTTCT TCTACTCTGT CCTCCTACTG 
GATGTGGTGT ACAGAGAAGA AACCCTGCTA AACGTTATCA GATCAGTCAC AAGGAACGGG 
CGGTCCATTG TCCTCACAGC AGTGCTGGCT GTCATCCTCA TCTACCTGTT CTCCATCATC 
GGATACCTGT TTTTTGAGGA TGACTTCCTG GTGGATGTCG ATCCAGCACC TCACCTGATG 
CCAGCTCTTG CTGGAGGAGG CATGATGTGT ACACAGGAGG GTGGTTGTGC AGGTGCAGAC 
TCCCCCATGG CAGCTATGTT TGGTTCCAAT AACACAGAGA ATGCGACAGA TGTGGTGCTG 
CCTGTGGTGG AGGAGGGTGG AGATGATCAG AAGGAGCGAG CCTGTGACAC CCTCATCATG 
TGTATCATCA CCACCCTGAA CCAGGGCCTG AGGAACGGGG GAGGCATCGG GGACGTCCTC 
AGGAACCCCT CCAGCAAGGA GTCATTGTTT GTGTTCCGAG TGATGTACGA CCTGCTCTTC 
TTCTTCGTCA TCATCATCAT CCTGCTGAAC CTCATCTTTG GTATCATCAT CGACACCTTC 
GCCGACCTGA GGAGTGAGAA GCAGCAGAAG GAAGAGATCC TGAAGAACAC TTGTTTCATC 
TGTGATGACA GAGCAGAGGA AACAGAAGCA GAGAATCGGT CTACTGGCTC CACCTGGCCT 
CCCACAGATC CCACCACAGT TCCAACCACA ACAACAACAG GTCCCACCAC CTGTAGCTGT 
CAACCCCTAA CCCTACAACT ATCAAGAACC AACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002599792.1
CDS	1..8139
Translation	MGEPSSFLHIGDIVSLFAEGSVSGFISTLGLVDDRCVVQPDAGDLSNPPKKFRDCLFRICPMNRYSAQKQFWKAAKPNPSNPADAVLLKKLQHAADLEKKQNEAENKKLLGSVIQYGGVIQSNKFLTVNKRLPAHLEKNAMRVTLDPSGNEGSWFYIQPFYKLRSPGDNVVVGDKVTLMPVNAGQPLHASNYELVDNPGCKEVNSVNSNTSWKVSLFMIYTENIDDVLKGGDVVRLFHAEQEKFLTGDEYKRKECVFLRVTARQSATSATSSKALWEVEVVHHDPCKGGAGHWNSLFRFKHLASGTYLAAEIDHDPTPDTQRQKLAGPQGGMVYQLMPIPHGHDIASIFELDPTTMQRADSMVPRSSYVRLRHLCTNTWVHSTAIPIDKEEDKPVMLKVGAAPIREDKEAFAIVPVPANEVRDLDFANDACKVMQDIASKLEKSSITQNERSSVSQLPTRPAINNVLSKSVRRSGCFLDLVLQQQPPLQGGLLTDLVYFLADKPNNGGDPLDVQITNPNRERQKLMRDQNILKQIFKVLQAPFEDKGDGPMLRMEELSDQRHAPFRHICRLCYRVLRLSQQDYRKNQEYIAKQFGFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIETFVSLVRQKKECRFLEYLSDLCVSNNQAIPVTQELICKSVLKPANSDILTETRIVKTEVEEVIEDEEGEDGEPITQIVEEEEVVLFWEKGHKHKAIRELAADAEESKEDKQVMDYYRYQLDLFSQMCLDRQYLAINILSTQLDINLILQCMSDESLPYDLRASFCRLMLHMHVDRDPQEQVTPVKYARLWSEIPSKITIEDYDSTRDKSKADMRPRFEDTMRFVERYLRHNLADNARGWQFADRNQNKLTFEVVNLSRHLVYFGFYSFSDLLRLTQTLLSILDITNSTTPVLALGKMDKVSELVGKTGVFKSLHDVSAVMTTMVLGGGGMSGGKALKAGDGEMDTIVMDTKLKIIEILQFILNVRLDYRISCLLSIFKNDFDESNSSQRGDTLPGLGTNAQDNSVVDLEKIAQQAESIFDGSDESADLDLDGAGGRTFLRVLLHLTMHDYPPLVSGALQLLFRHFSQRQEVLQAFKQVQLLVSSSDVENYKQIKADLDELRLLVEKSELWVYKSKSEGGKKEKKESSTGDGDGDKKKKSEGGADKKEGKSSLTSSQGPEGSAIDLDIGPSMDQNSVKNYKTIQQVLIRLSQLCVHQSSGKNRKHEQRLLRNMGAHSVVLELLQIPFDKKEDTRMNEIMRLAHEFLQKFCQGNPANQALLHKHVDLFLTPGLLEAETMRHIFMDNSMLCNEVTERVVQHFVHCIETHGRHVQYLKFLQTIVKAEGQYIRKCQDMVMAELVNVGEDVLLFYNDRSSFNTLIEMMRSEREKRQLSSPLMYHINLVQLLGLCTEGKNVYTEIKCHSLLPLDDISRVVVHPDCLPEVKDAYINFLNHCYVDTEVEMKEIYTSNHMWTLFENFLVDMATVCKATDRNHADPVLEHYVTNTIMNIITMFFSSPFSDQSTTVQTRQPIFVQLLSGAYRVSQCTWLNTQQRCMVETCIKTLTEIAKNRGIAIPVDLDSQVNTLFSKSQLLMATSRKWLSTRGSSRRDSIMAISRDTRSIIEGLQVSVFSHLSFPLTSLSQQWQDLVPPPMAANRRIVELTTKNLPQDIVSLLEDQLRPLVQAELSVLVDVLHRPELLFPPGSEARQNCESGGFISKLIKHTERLLEEKEEKLCIKVLQTLKEMMTTDIDYGEKGDKLRSSLLTRYYGKQHPRADRNRKESGSPNAQPPQRPSVANPAPGAQMVGRAEMSLAEVQCHLDREGASLLVTDLVINSTSNRVFEEAVELGIALLEGGNSTIQKSFLNHLRDEGKAEKFFKVFYDRMRESQQEIKATVTVSTGDSLSGNKDNKDVAVKDTPAKMKKGAGGGSPGTAMSDDVRDQLADAALQTSKAFGAMRQGPQGEDAANPLSGFDKETEKKNEERGMSPEIAIMKPILRFLQLLCENHNRDLQNYLRNQNNKTNYNLVCETLQFLDCICGSTTGGLGLLGLYINEKNVDLINQTLASLTEYCQGPCHENQNSIACHECNGIDIITALILNDINPLGTHRMDLVLELKNNASKLLLAIMESRHDSENAERILYNVSPKQLVDVIKRAYMQEEMKEDEEDEESEEDEEEDKVSPKEVGHNIYILAHQLSQHNKELAALLKPSSAADYGNTALEYYAKHTAQIEIVRQDRTMERIVFPVPSICEYLTEETKSRVYHTAERDEQGSKVSDFFERCDDMFNEMKWQKKLRANPTLYWISAHMSLWSAISFNLAVLINLLIAFFFPFSGGTGELDPRLSGLVWTAMLVSLATVITLPKASGIRTLVASTILRLIFSVGLEPTLWLIGCANVINKAVFLVSLLGNRGTFTKNLQAIMGDVELWYYVAYLILCVLGLTMHEFFYSVLLLDVVYREETLLNVIRSVTRNGRSIVLTAVLAVILIYLFSIIGYLFFEDDFLVDVDPAPHLMPALAGGGMMCTQEGGCAGADSPMAAMFGSNNTENATDVVLPVVEEGGDDQKERACDTLIMCIITTLNQGLRNGGGIGDVLRNPSSKESLFVFRVMYDLLFFFVIIIILLNLIFGIIIDTFADLRSEKQQKEEILKNTCFICDDRAEETEAENRSTGSTWPPTDPTTVPTTTTTGPTTCSCQPLTLQLSRTNY

Target ORF information:

RefSeq Version	XM_002599746.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002599746.1

ORF Insert Sequence:

ATGGGGGAAC CGTCGAGCTT TCTCCACATC GGCGACATCG TGTCCCTGTT CGCCGAGGGC 
TCTGTCAGCG GTTTCATATC AACTCTTGGT CTTGTTGATG ACCGATGCGT GGTGCAGCCA 
GACGCGGGCG ACCTGTCCAA CCCGCCCAAG AAGTTCCGAG ACTGCCTGTT CCGGATCTGT 
CCTATGAACC GGTATTCTGC CCAGAAGCAG TTCTGGAAGG CAGCCAAACC CAACCCCTCC 
AACCCTGCTG ATGCTGTCCT GCTCAAAAAG CTACAGCATG CAGCAGACCT GGAGAAGAAA 
CAAAATGAGG CTGAGAACAA GAAGCTACTT GGCAGTGTCA TTCAGTATGG AGGAGTCATA 
CAGAGCAACA AGTTCCTGAC AGTGAACAAG CGCCTCCCGG CACATCTGGA GAAAAATGCA 
ATGAGAGTCA CCCTTGATCC ATCCGGAAAT GAAGGGTCAT GGTTTTACAT CCAGCCCTTT 
TACAAGCTGA GATCACCAGG GGATAATGTT GTTGTTGGTG ACAAGGTGAC CCTGATGCCA 
GTCAATGCTG GACAGCCTCT CCATGCTAGT AACTATGAGC TGGTGGACAA CCCTGGTTGT 
AAAGAGGTGA ACTCTGTAAA CTCTAATACG AGTTGGAAGG TATCGCTCTT CATGATCTAC 
ACAGAGAACA TTGATGATGT GCTGAAGGGA GGGGACGTGG TACGGCTATT CCATGCTGAG 
CAGGAGAAGT TCCTGACAGG AGATGAGTAC AAGAGGAAGG AGTGTGTGTT CCTGAGGGTC 
ACCGCCAGAC AATCAGCAAC CTCAGCTACA AGTTCCAAAG CCCTGTGGGA AGTTGAGGTG 
GTTCATCACG ACCCCTGTAA GGGTGGCGCC GGTCACTGGA ACAGCCTCTT CAGGTTCAAA 
CACCTGGCCT CTGGGACCTA CCTGGCAGCA GAGATTGACC ATGACCCGAC CCCTGACACC 
CAGCGGCAGA AGCTGGCGGG GCCGCAGGGC GGGATGGTTT ACCAGCTGAT GCCCATACCC 
CACGGGCACG ACATCGCCTC CATCTTTGAA CTGGATCCCA CCACCATGCA GAGAGCAGAC 
TCAATGGTTC CGAGGAGCTC CTACGTGCGT CTGCGACACC TGTGCACGAA CACCTGGGTA 
CACAGCACAG CCATACCTAT AGACAAGGAG GAGGACAAAC CTGTCATGCT CAAGGTTGGT 
GCTGCTCCAA TCCGGGAGGA TAAGGAAGCC TTTGCCATTG TTCCTGTCCC TGCCAACGAG 
GTCAGAGACC TGGACTTTGC CAATGACGCC TGCAAGGTGA TGCAGGACAT AGCTAGCAAA 
CTAGAGAAAA GCAGCATCAC TCAGAACGAG AGAAGTTCTG TTTCCCAACT GCCAACTCGG 
CCGGCCATCA ACAATGTGCT GTCAAAGAGC GTCCGGAGGT CTGGCTGTTT TCTGGACTTG 
GTCCTGCAAC AACAGCCTCC CCTGCAGGGA GGACTGCTGA CGGACCTGGT GTATTTCCTG 
GCAGACAAGC CCAACAACGG AGGGGACCCC CTGGACGTGC AGATCACCAA CCCAAACAGG 
GAGAGGCAGA AGCTCATGAG AGATCAGAAC ATTCTTAAAC AGATCTTCAA AGTTCTCCAG 
GCGCCCTTTG AGGACAAGGG TGATGGTCCT ATGCTGCGGA TGGAGGAGCT GTCTGACCAG 
AGACACGCCC CCTTCAGGCA CATCTGCAGG CTGTGCTACA GGGTGCTCAG GCTGTCACAG 
CAGGACTACA GGAAGAACCA GGAGTACATA GCAAAGCAGT TTGGTTTTAT GCAAAAGCAG 
ATCGGCTATG ATGTGTTGGC TGAAGACACC ATCACAGCTC TTCTGCACAA TAACAGAAAA 
CTGCTGGAAA AGCATATCAC CGCTGCTGAG ATAGAGACCT TCGTTAGCCT TGTTAGGCAG 
AAGAAGGAAT GCAGATTTCT GGAATACCTG TCAGACCTGT GTGTGTCCAA CAACCAGGCC 
ATCCCTGTCA CCCAGGAGCT GATCTGTAAG TCTGTCCTCA AGCCAGCCAA CAGTGACATC 
CTCACGGAAA CTAGAATTGT GAAGACAGAA GTGGAGGAGG TGATAGAGGA TGAGGAGGGT 
GAGGATGGAG AGCCCATCAC CCAGATTGTG GAGGAGGAGG AGGTGGTGCT GTTCTGGGAG 
AAGGGACACA AACATAAGGC CATCCGGGAG CTGGCCGCTG ATGCCGAGGA GTCTAAGGAA 
GACAAGCAAG TTATGGACTA CTACAGATAC CAGCTGGACC TGTTCTCCCA GATGTGCCTG 
GACAGACAGT ACCTCGCCAT CAACATCCTC TCCACACAAC TGGACATCAA CCTTATATTG 
CAGTGCATGT CGGATGAGTC GTTGCCGTAC GACCTTCGCG CTTCGTTCTG CCGGCTGATG 
CTGCACATGC ATGTGGACAG GGACCCCCAG GAGCAGGTCA CCCCTGTCAA GTATGCACGC 
CTCTGGTCCG AGATCCCCTC CAAAATCACG ATAGAGGACT ATGACTCCAC CAGGGACAAG 
AGTAAGGCGG ACATGAGACC CAGGTTTGAG GACACCATGC GGTTTGTAGA GCGCTACCTC 
CGGCATAACC TTGCCGACAA TGCACGAGGA TGGCAGTTCG CTGATCGGAA CCAGAACAAA 
CTAACATTTG AGGTGGTGAA CCTGTCCCGA CACCTGGTGT ACTTCGGGTT CTACAGCTTC 
AGCGACCTGC TGAGACTGAC CCAGACCCTC CTGTCCATCC TGGACATCAC CAACTCCACC 
ACACCCGTCC TGGCGCTGGG CAAGATGGAC AAGGTGTCCG AGCTAGTGGG TAAGACGGGA 
GTGTTCAAGT CCCTGCACGA CGTGAGCGCT GTGATGACCA CCATGGTGCT GGGGGGAGGT 
GGCATGTCCG GGGGGAAGGC TCTCAAGGCT GGGGACGGAG AGATGGACAC CATCGTCATG 
GATACCAAAC TGAAGATCAT TGAAATTCTG CAGTTTATCC TGAACGTCCG TCTGGACTAC 
CGCATCTCCT GTCTCCTGTC CATCTTCAAG AACGACTTTG ATGAGAGTAA CAGCAGCCAG 
CGTGGTGATA CCCTCCCTGG GCTGGGGACT AATGCACAGG ACAACTCTGT GGTAGATTTA 
GAGAAGATTG CACAACAAGC AGAAAGCATC TTTGATGGCA GTGATGAGTC AGCAGATCTG 
GACCTGGATG GTGCGGGAGG GCGGACGTTC CTGAGAGTCC TGCTGCACCT GACCATGCAT 
GACTACCCAC CCCTGGTGTC AGGGGCGCTG CAGCTGCTCT TCAGACACTT CAGCCAAAGG 
CAGGAGGTGC TGCAGGCCTT CAAACAGGTA CAACTGCTGG TCTCCTCCAG CGATGTGGAG 
AACTACAAGC AGATCAAAGC TGACCTGGAC GAGCTCAGAC TGCTTGTGGA GAAGTCCGAA 
CTCTGGGTCT ACAAGAGCAA GAGTGAAGGG GGCAAAAAGG AGAAGAAGGA GTCCAGTACA 
GGGGATGGGG ACGGAGACAA GAAGAAGAAA TCTGAGGGTG GGGCTGACAA AAAGGAGGGG 
AAATCGTCCT TGACATCATC CCAAGGTCCA GAAGGTTCTG CTATTGACCT TGATATTGGA 
CCTTCCATGG ATCAAAATAG CGTCAAGAAT TACAAGACCA TCCAGCAGGT TCTGATTCGC 
CTGAGCCAGC TTTGCGTCCA CCAGTCCAGC GGGAAGAACC GTAAACACGA GCAGCGTCTG 
CTGAGGAACA TGGGAGCCCA CAGTGTTGTA CTGGAACTCC TCCAGATCCC CTTTGATAAG 
AAGGAAGACA CCAGGATGAA CGAGATCATG CGGTTAGCCC ATGAGTTCCT TCAGAAGTTC 
TGCCAAGGAA ATCCAGCCAA TCAGGCACTG CTACACAAAC ATGTGGACCT GTTCCTGACT 
CCTGGGCTCT TGGAGGCAGA AACTATGAGA CACATATTCA TGGACAACAG CATGCTGTGC 
AATGAAGTGA CAGAGAGAGT GGTGCAGCAC TTTGTTCACT GCATCGAGAC CCACGGTCGC 
CATGTGCAGT ATCTGAAGTT CCTACAGACC ATTGTGAAAG CAGAGGGACA ATACATCAGG 
AAATGTCAAG ACATGGTCAT GGCAGAGTTG GTGAATGTAG GAGAAGACGT CCTGCTGTTC 
TATAACGACA GGTCGTCCTT CAACACCCTG ATCGAGATGA TGCGGTCGGA GCGAGAGAAG 
AGACAGCTGA GCAGCCCTCT GATGTACCAC ATCAACCTGG TGCAGCTGCT GGGGCTGTGC 
ACCGAGGGCA AGAACGTGTA CACAGAGATC AAGTGCCACT CCCTGCTCCC TCTGGATGAT 
ATCAGCAGGG TGGTGGTGCA TCCGGACTGT CTACCTGAGG TTAAAGATGC TTACATCAAC 
TTCCTGAACC ACTGCTATGT GGACACTGAG GTGGAGATGA AGGAGATCTA TACCAGCAAC 
CACATGTGGA CTTTGTTTGA GAACTTCCTT GTCGACATGG CCACGGTTTG CAAAGCCACA 
GATCGTAACC ACGCCGACCC AGTTCTAGAG CATTACGTCA CCAACACCAT CATGAACATC 
ATCACCATGT TCTTCAGCTC CCCCTTCTCA GACCAGAGCA CCACAGTTCA GACCCGACAG 
CCAATCTTCG TCCAACTGCT GTCTGGAGCG TATCGTGTGT CGCAGTGTAC ATGGCTGAAT 
ACACAGCAGC GCTGCATGGT GGAGACATGC ATCAAAACCC TGACAGAAAT AGCCAAGAAC 
CGTGGCATAG CCATCCCTGT GGACTTGGAC AGCCAGGTGA ACACACTGTT CTCCAAGTCC 
CAACTCCTCA TGGCCACATC CCGTAAGTGG CTGAGTACGC GTGGCTCGTC CAGACGAGAC 
TCCATCATGG CCATTTCACG GGACACCAGG AGCATTATTG AAGGGTTGCA GGTCAGTGTT 
TTTTCTCATC TTTCCTTTCC TCTCACCTCC CTGAGTCAGC AGTGGCAGGA CTTGGTGCCT 
CCTCCCATGG CTGCCAACAG GCGGATTGTG GAGCTAACAA CCAAAAACCT GCCACAGGAC 
ATTGTGTCCT TGCTGGAGGA CCAGCTGCGG CCGCTGGTCC AGGCGGAGCT GTCCGTGCTG 
GTGGACGTGC TGCACAGACC CGAGCTGCTG TTCCCACCGG GATCGGAGGC CAGACAGAAC 
TGTGAATCTG GCGGATTCAT CTCCAAACTT ATCAAACACA CGGAGAGGCT GCTAGAAGAG 
AAGGAGGAGA AACTGTGCAT CAAGGTGCTG CAAACTCTCA AGGAAATGAT GACCACAGAC 
ATTGACTATG GAGAAAAGGG AGACAAGTTA CGCAGCAGCT TGTTGACACG GTACTACGGA 
AAGCAGCACC CGCGAGCAGA CCGCAACCGC AAGGAGAGCG GATCTCCGAA TGCGCAGCCG 
CCCCAGCGCC CGTCTGTGGC CAACCCCGCC CCTGGGGCAC AGATGGTGGG GAGGGCAGAG 
ATGTCCCTGG CGGAGGTGCA GTGCCACTTG GACAGAGAGG GGGCATCGCT TCTGGTCACA 
GACTTGGTCA TCAATAGCAC CAGCAACAGG GTGTTTGAGG AAGCAGTGGA GCTTGGGATA 
GCATTACTGG AGGGTGGCAA CAGCACCATA CAGAAATCTT TCCTGAACCA CCTGCGGGAT 
GAGGGGAAGG CAGAGAAGTT CTTTAAGGTT TTCTATGACC GGATGAGGGA GTCTCAGCAG 
GAGATCAAGG CCACAGTCAC TGTTAGCACC GGCGACAGTC TGTCAGGCAA CAAGGACAAC 
AAAGATGTGG CAGTGAAGGA CACACCAGCA AAAATGAAGA AAGGTGCAGG TGGAGGCAGC 
CCAGGGACGG CCATGTCAGA CGATGTGAGG GACCAGCTAG CTGATGCAGC GCTGCAGACC 
AGCAAAGCCT TCGGAGCCAT GAGGCAGGGA CCACAGGGGG AGGACGCTGC CAACCCTCTC 
TCAGGATTTG ACAAGGAGAC AGAGAAGAAG AACGAGGAGC GAGGCATGAG CCCTGAGATC 
GCCATCATGA AGCCCATCCT GAGGTTCCTG CAGCTGCTGT GTGAGAACCA CAACAGGGAC 
CTGCAGAATT ACCTGCGGAA CCAGAACAAC AAGACCAACT ACAACCTTGT GTGTGAGACT 
CTGCAGTTCC TGGACTGCAT CTGTGGCAGC ACCACCGGTG GGCTGGGCCT GCTGGGGCTG 
TACATCAACG AGAAGAACGT CGATCTCATC AACCAGACAC TGGCCAGTCT GACCGAGTAC 
TGCCAGGGAC CCTGCCACGA GAATCAGAAC TCTATTGCCT GCCATGAGTG TAATGGAATT 
GACATCATCA CAGCTTTGAT TCTTAATGAC ATCAACCCTC TGGGTACACA CCGTATGGAC 
TTGGTGTTAG AGCTCAAGAA CAATGCCAGT AAGCTGCTAC TTGCTATCAT GGAAAGCCGA 
CATGACAGTG AGAATGCTGA AAGGATCTTG TACAACGTCA GTCCAAAGCA GTTGGTTGAT 
GTGATTAAGA GGGCGTACAT GCAGGAGGAG ATGAAGGAGG ATGAGGAGGA TGAGGAAAGT 
GAAGAGGATG AGGAAGAGGA CAAAGTCTCA CCGAAGGAAG TTGGACACAA CATCTATATC 
TTGGCACATC AGCTCTCGCA GCACAACAAG GAGCTGGCAG CCCTGCTGAA ACCCAGTAGT 
GCTGCAGACT ACGGCAACAC AGCCCTGGAG TACTATGCCA AACACACTGC ACAAATTGAG 
ATTGTGCGTC AGGACAGGAC CATGGAGAGG ATTGTGTTCC CTGTGCCCAG TATCTGTGAG 
TACCTGACGG AGGAGACAAA GTCACGTGTG TACCACACCG CCGAGAGGGA TGAGCAGGGC 
AGCAAGGTGT CCGACTTCTT CGAGAGATGT GACGACATGT TCAATGAGAT GAAGTGGCAG 
AAAAAACTCA GAGCCAACCC CACCCTGTAC TGGATCAGTG CTCACATGTC CCTGTGGAGC 
GCCATCTCCT TCAACCTGGC TGTGCTCATC AACCTCCTCA TCGCCTTCTT CTTCCCCTTC 
TCAGGAGGAA CTGGAGAACT TGACCCTCGG CTGTCTGGTC TGGTGTGGAC GGCCATGCTG 
GTTTCCCTGG CAACAGTCAT CACCCTACCC AAAGCCTCCG GGATACGAAC ACTGGTGGCC 
TCCACTATAC TCAGGCTGAT ATTCAGTGTG GGGCTGGAGC CAACCCTGTG GTTGATCGGC 
TGTGCAAATG TGATAAACAA GGCAGTGTTC CTGGTGAGCC TGCTGGGGAA CAGAGGGACC 
TTCACCAAGA ACCTGCAGGC CATCATGGGG GATGTGGAGC TGTGGTACTA CGTGGCGTAC 
CTCATCCTCT GTGTGCTGGG CCTCACCATG CACGAGTTCT TCTACTCTGT CCTCCTACTG 
GATGTGGTGT ACAGAGAAGA AACCCTGCTA AACGTTATCA GATCAGTCAC AAGGAACGGG 
CGGTCCATTG TCCTCACAGC AGTGCTGGCT GTCATCCTCA TCTACCTGTT CTCCATCATC 
GGATACCTGT TTTTTGAGGA TGACTTCCTG GTGGATGTCG ATCCAGCACC TCACCTGATG 
CCAGCTCTTG CTGGAGGAGG CATGATGTGT ACACAGGAGG GTGGTTGTGC AGGTGCAGAC 
TCCCCCATGG CAGCTATGTT TGGTTCCAAT AACACAGAGA ATGCGACAGA TGTGGTGCTG 
CCTGTGGTGG AGGAGGGTGG AGATGATCAG AAGGAGCGAG CCTGTGACAC CCTCATCATG 
TGTATCATCA CCACCCTGAA CCAGGGCCTG AGGAACGGGG GAGGCATCGG GGACGTCCTC 
AGGAACCCCT CCAGCAAGGA GTCATTGTTT GTGTTCCGAG TGATGTACGA CCTGCTCTTC 
TTCTTCGTCA TCATCATCAT CCTGCTGAAC CTCATCTTTG GTATCATCAT CGACACCTTC 
GCCGACCTGA GGAGTGAGAA GCAGCAGAAG GAAGAGATCC TGAAGAACAC TTGTTTCATC 
TGTGATGACA GAGCAGAGGA AACAGAAGCA GAGAATCGGT CTACTGGCTC CACCTGGCCT 
CCCACAGATC CCACCACAGT TCCAACCACA ACAACAACAG GTCCCACCAC CTGTAGCTGT 
CAACCCCTAA CCCTACAACT ATCAAGAACC AACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_70263 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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