BRAFLDRAFT_65941 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_65941
Entrez Gene ID	7214160
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002610666.1	XP_002610712.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_65941 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_65941 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb03973	XM_002610666.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_65941 ( XM_002610666.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002610712.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb03973
Clone ID Related Accession (Same CDS sequence)	XM_002610666.1
Accession Version	XM_002610666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101546). COMPLETENESS: incomplete on both ends.

ATGCTGAAGA CGTTTGATCA CTACTGCGAG TACCGGAGGA CCCCCCACGG ACTGGTGCTG 
TCTCCCATCC AGCTGGGCAA GTGGCTGGTG GTCTTCTGTG ATGAGATCAA CCTGCCAGAC 
ATGGACAAGT ATGGCACGCA GCGTGTCATC TCCTTCCTGC GCCAGATGGT GGAGCACGGA 
GGCTTCTTCC GCTCAGCGGA CCAGCAGTGG GTCAAGTTCG AGAGGATCCA GTTTGTGGGA 
GCGTGCAATC CCCCAACGGA CCCAGGCCGC AAGCCTTTGT CTCACAGGTT CCTACGTCAC 
GTCCCTGTGG TGTATGTGGA CTACCCTGCA GCCCCCTCCC TCATGCAGAT CTACGGCACC 
TTCAACCGTG CCATGCTGCG CCTCATCCCC ACCCTGCGTA CCTACGCAGA GCCTCTCACT 
ATGGCAATGG TGGAGTTCTT CACTATGTCA CAGGAGAGGT TTACTCAGGA CACCCAGCCA 
CACTACATCT ACAGCCCACG TGAAATGACC CGCTGGGTGC GAGGCATCCT GGAGGCGCTG 
CGGCCCCTGG AGTCCCTCCC CGTGGAGGGG CTGGTGCGCA TCTGGGCCCA CGAGGGCCTC 
AGGCTCTTCC AGGACAGGCT GGTGGAAGAG GAGGAGCGTC AGTGGACAGA TGAGAACATC 
AACATGGTGG CGCTCAAACA CTTCCCCAAC ATCGACCAGC AGAAGGCTCT GGGTCGCCCC 
ATCCTATACA GCAAGTGGCT GTCAAAGGAC TACGTACCTG TGGACAGGGA GGAGCTGCGA 
GAGTACACCA AGGCTCGCCT CAAGGTGTTC TATGAAGAGG AGCTTGACGT GCCGCTGGTC 
CTGTTTAACG AGGTTCTGGA CAACGTCCTG CGCATCGACC GGATCTTCCA GCAGCCACAA 
GGTCACCTGC TGCTGATCGG AATGAGCGGG GCTGGCAAGA CGACCCTGTC GCGCTTCGTC 
GCCTGGATGA ACGGACTGAC TGTGTATCAG ATCAAGGTCC ACAGGAAGTA CATGGCAGCA 
GACTTTGATG AGGACCTGAG GAATGTGCTG AGACGGTCGG GCTGTAAGGA CGAGAAGATC 
GCCTTCATCA TGGATGAGTC CAACGTGCTG GACTCGGGCT TCCTGGAGAG GATCAACACT 
CTTCTGGCAA ACGGAGAGGT GCCAGGACTG TTTGAAGGAG ACGAGTACAC CACCCTGATG 
ACCCAGTGTA AGGAGGGTGC CCAGCGGAAC GGGCTGATGT TAGACACCGG CGAGGAGCTG 
TACAAGTGGT TCACCCAGCA GGTCATGAGG AACCTACACG TGGTCTTCAC CATGAACCCA 
TCCTCCCAGG GTCTGAAGGA CCGGGCTGCC ACGTCACCCG CCCTCTTCAA CAGATGTGTG 
CTGAACTGGT TCGGAGACTG GTCAGAGGGA GCCCTGTACC ACGTCGGCCT GGAGTTCACC 
AGCAAGTTGG ACCTGGAGCG GCCGGACTGG AAGCTGCCCG ACTACATCCC CCAGGCGTTC 
GAGGGGCTGC CTGACATGCC GACCCACAGG GACATCATCG CCAACGCCTT CGTCTACGTG 
CACCAGACGC TGCACCAGGC CAACCAGAGG CTGAGCAAAC GTGGCGCCCG CATCATGGCC 
ATCACACCTC GCCATTACCT GGACTTCATC AACCACTATG TGAAACTGTA CAACGAGAAG 
CGGTCTGACC TGGAGGAGCA GCAGCTCCAC CTGAACGTGG GCCTGTCCAA GATCCGGGAG 
ACTGTGGAGC AGGTGGAGGA GATGAGGAAG AGCCTGTCAG TCAAGAAGCA GGAGCTGGAG 
GCCAAGAACA ACCTGGCCAA CGCCAAGCTG AAGGAGATGG TGAAGGACCA GCAGGAGGCT 
GAGAGGAAGA AGGTGATGTC CATAGAAATC CAAGAGAAGC TGAAGACGCA GCTGGAACAG 
ATCAAGGTGA AGCGTGCGTA TGTGATGGAG GACCTGGCCA AGGTCGAGCC CGCTGTGATC 
GAGGCCCAGC AAGCTGTCAA GGGCATCAAG AAACAGCATC TGGTGGAGGT ACGTTCCATG 
GTGAACCCCC CTGCTGCAGT CAAGATGGCC CTGGAGTCCA TCTGCCTTCT AATGGGAGAA 
CCCACATCGG ACTGGAAGGC CATCCGTGGA ATTGTCATCA GGGACAACTT CATCAGCACC 
ATCGTCAACT TCCAGACTGA TGACATCTCA GACAACATTC GGGAGACCAT GGTGAGCAAG 
TACCTGTCCG AACCCAAGTA CAACTTTGAG ACTGTGGACA GGGCCAGCAA GGCCTGTGGG 
CCCATGGTCA AGTGGGCAAT TGCACAGGTA AACTATGCAG ACATGCTGCA GCGCGTGGAC 
CCGCTGCGAC AGGAGCTGCG CTCGCTGGAA GAGGAGGCCG ACAGGAACAA GATGGAGGGA 
GAGGAGATGA ACAGGCTGGT CCAGGAGCTC GAGAAGAGCA TCGCCAGGTA CAAGGAGGAG 
TATGCCGTGC TGATCAGCCA GGCGGAGGCC ATCAAGGCCA GCCTGCTGGC TGTGGAAGAG 
AAGGTGACCC GTGCCACGGA TCTCCTGAGG AGCCTGGGTA TGGAGAGGGA GCGATGGGAG 
GCCGGCAGCG TGTCCTTCAA GAACCAGATG GCCACCATCG TCGGAGACTG CCTGCTCTCC 
GCCGCCTTCC TGGCCTACGC GGGTTACTTC GACCAGCACA TGAGGCAGAG TCTGTTCAAC 
TCCTGGTCGC ACCACCTGCA GCAGGCCAAC GTGAAGTTCC GCCCGGACAT TGCCCGCGTG 
GAGTACCTCT CCAACGCCGA CGAGCGGCTG CGCTGGCAGG CCAACGCCCT CCCGGCCGAC 
GACCTCTGCA CAGAGAACGC CATCATGCTG AAGCGCTTCA ACCGCTACCC GCTGATCATC 
GACCCGTCCG GGCAGGCCAC GGCGTTCGTC ATGAACGAGT ACCGGGAGAG GACCATCACC 
AAGACCAGCT TCCTGGACGA CGCCTTCCGC AAGAACCTGG AGAGCGCGCT GCGCTTCGGG 
AACCCCCTGC TGGTGCAGGA CGTGGAGAGC TACGACCCCA TCCTCAATCC CGTGCTGAAC 
CGCGAGGTCC GACGCACCGG GGGCCGCGTG CTCATCACGC TGGGAGACCA GGACATTGAT 
CTGTCGCCTT CCTTCACCAT CTTCCTGTCC ACCCGGGATC CCACCGTGGA GTTCCCCCCG 
GACATCTGCT CCAGGGTGAC CTTCGTGAAC TTCACGGTGA CCCGCAGCAG TCTGCAGAGC 
CAGTGTCTGA ACGAGGTCCT GAAGGCAGAG CGGCCGGACG TCGACGAGAA GAGATCCGAC 
CTGCTGAAGC TGCAAGGTGA GTTCCACCTG CGTCTGCGCC AGCTGGAGAA GTCCCTGCTC 
CAGGCCCTGA ACGACGTGAA GGGCAAGATC CTGGACGACG ATCGCATCAT CACCCACCTG 
GAGACTCTGA AGAAGGAGGC CGCGGAGATC ACACGCAAGG TTGAGGAGAC AGATGCGGTG 
ATGGCTGAGG TGGATCGCGT GTCCCAGCAG TACCATCCCC TGGCAGCAGC CTGCAGCAGC 
ATCTACTTCA CCATGGACTC CACTCACCAG ATCCACTTCC TGTACCAGTA CTCCCTGCAG 
TTCTTCCTGG ATATCTACCA CGCAGTCCTG TATGAGAACT CCCACCTTCA GGGGGTGACA 
GACTACATGG CTCGCCTCAA CGTCATCATC AAGGACCTCT TCCAGGAGTC GTTCAAGCGC 
GTTGCCCGAG GCATGCTGTT CAAGGACAGG GTCACCTTTG CTGTGCTGCT GGTGCGCATC 
TTCCTGAAGG GAGTTGTGGA CGAGCCGACG GAGTACGATG CAGAGTTCCA GCACCTGCTG 
CGCGGGAAGG AGACGGTGGT GAAGAGCCAT GAGCTGCCAA TCATCGAGGG ACTGGCCACA 
GAACAGCTGG AGAGCATGAT GCGCCTGACC CACCTGCCCT CCTTCAGTAA CCTGGCCGTC 
CGTGTCCAGG CCGAGGACGC CTTCTCCTCC TGGGTGGAGT CGGTCACTCC GGAGGTGGAC 
GTTCCTGTGC TGTGGGCTGA TGACGACACG CTCACTAAGA CTGGGCGCGA CATGCAGCGA 
CTGCTGGTGA TCCAGGCCTT CCGTCCGGAC CGCCTGCGGG CCATCACCCA CAGGTTCGTG 
CAGACCGTGA TGGGAGACTC TTTCATGCAC TGTGCCGAGC AGGAGCTGGA CCTGGCTGCG 
ACTGTGGAGA ATGAGATCAA GTCCAACACC CCCCTCCTGC TGTGCTCTGT ACCCGGCTAC 
GATGCCAGCG GACATGTGGA GGATGTGGCA GCTGAGCTGG GCAAGGGTCT GACTCCCATT 
GCCATCGGGT CTGCTGAAGG ATTCAGCCTG GCCGACAAAG CCATCAACAC TGCCACCAAG 
TCTGGAAGGT GGGTGCTGTT GAAGAACGTC CATCTGGCTC CCCAGTGGCT GGTGCAGCTG 
GAGAAGAAGC TCCACTCACT CACACCGCAC CGCGACTTCC GCCTCTTCCT CACCATGGAG 
ATCAACCCCA AGGTGCCGGT GAACCTGCTG CGTGCCGGCC GTGTGTTTGT GTTTGAACCT 
CCTCCTGGTG TGAAGGCCAA CCTGCTGGGG ACCTTCAGCA CCATCCCTGC TGCACGCATG 
TGCAAGGCGC CTGGCGAGCG AGCACGGCTG TACTTCCTGC TGGCCTGGTT CCACGCTGTG 
GTGCAGGAGC GGCTCCGCTA CGCTCCCCTG GGATGGTCTA AGTGCTACGA GTTCAACGAG 
TCGGACCTCC GCTGTTCCTG CGATACGCTG GACACCTGGA TCGAAATGGC TGCAAAGGGT 
CGAGCTAACC TCCCACCAGA CAAGGTGCCG TGGGACGCCC TGCGTATACT GCTGTCCCAG 
GCCATCTATG GAGGGCGTGT TGACAATGAC TTTGATCAGC GCCTGTTGAA TGCCTTCCTG 
GACAAGCTGT TCACAGCCAG CAGCTTTGAG AGAGACTTCC AGCTGGTCAG CAAGGTGGAT 
GGGAACAAGG ACATCATCAT GCCTGAAGGC ATCCATCGGG ACCAGTTCCT GCAGTGGTCG 
GAGGGGCTGC CCGACATGCA GACTCCGTCC TGGCTAGGAC TGCCCAACAA TGCTGAGAAA 
GTGCTGCTCA CAACACAAGG GGCGGACATG ATCATCAACC TGCTGCGGAT GCAGATGCTG 
GAGGATGATG AGGATGTGAT GGAGGAGGGC CGACCCCGGG CCGGCAGCAG GAAGCGCGCC 
ACCAGCAGCG GGGACGGCCG CCCCGCCTGG ATGCGCACCC TGCTCATGTC TGCCTCCAAC 
TGGCTCTCAC AGATTCCTAA GAATTTGACA GTCCTTCGTC GAACAGCAGA AAACATCAAG 
GACCCTCTGT TCCGATTCTT CGAACGTGAG GTGAATGCTG GGTCTCGGCT GTTGAAGGAG 
GTCCGCACCA ACCTGGAGAA CGTGGTTCTG GTGTGTGAAG GCAAGAAGAA GCAGACCAAT 
GAAGACAGGG AGCTCATCAG TGTTCTGGCC AAAGGCATGC TGCCGCAGTC GTGGCGTCGG 
TACAAGGTTC CTGATGGCAT GACAGTTATC CAGTGGGTTA TAGACTTCGG CCAGCGTATC 
AAGCAGCTGC ACAAGATCTC CCAGGCCACA GCTCAGGGTG GGGCCAAGGA ACTTAAGAAC 
CAGCAGATCT GGCTGGGCGG TCTGTTTGTC CCCGAGGCCT TCATCACGGC CACCCGCCAG 
TACGTGTCGC AGGCCAACAG CTGGTCCCTG GAGGAGCTCG CAATGGAGGT GCACATCTCT 
GACGGCAGCG GCAAGGACAT CCCCAGGGAC GGCGGCACCT TCATGGTCAC AGGTTTGAAA 
CTCCAGGGAG CTGTCTGTAA GCAGAACACC CTGCAGCTGA GTAAGACCAT CTCTACTGAG 
CTGCCCATCA CCTGCCTCCG CTGGATCAGG TACAGACATT TATTCATTTT TTTTATTTCA 
GTGACCCTCC CGGTGTACCT GAACCAGACC CGTGCTGACC TGCTGTTCAC GGTGGACCTG 
CAGACCGCAG GCAGCCGTTC TGGCGCCAAC CATGGCTTCT ACGAGCATGG TGTGGCCCTG 
CTGGCAGCCG AGTGGATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002610712.1
CDS	1..6081
Translation	MLKTFDHYCEYRRTPHGLVLSPIQLGKWLVVFCDEINLPDMDKYGTQRVISFLRQMVEHGGFFRSADQQWVKFERIQFVGACNPPTDPGRKPLSHRFLRHVPVVYVDYPAAPSLMQIYGTFNRAMLRLIPTLRTYAEPLTMAMVEFFTMSQERFTQDTQPHYIYSPREMTRWVRGILEALRPLESLPVEGLVRIWAHEGLRLFQDRLVEEEERQWTDENINMVALKHFPNIDQQKALGRPILYSKWLSKDYVPVDREELREYTKARLKVFYEEELDVPLVLFNEVLDNVLRIDRIFQQPQGHLLLIGMSGAGKTTLSRFVAWMNGLTVYQIKVHRKYMAADFDEDLRNVLRRSGCKDEKIAFIMDESNVLDSGFLERINTLLANGEVPGLFEGDEYTTLMTQCKEGAQRNGLMLDTGEELYKWFTQQVMRNLHVVFTMNPSSQGLKDRAATSPALFNRCVLNWFGDWSEGALYHVGLEFTSKLDLERPDWKLPDYIPQAFEGLPDMPTHRDIIANAFVYVHQTLHQANQRLSKRGARIMAITPRHYLDFINHYVKLYNEKRSDLEEQQLHLNVGLSKIRETVEQVEEMRKSLSVKKQELEAKNNLANAKLKEMVKDQQEAERKKVMSIEIQEKLKTQLEQIKVKRAYVMEDLAKVEPAVIEAQQAVKGIKKQHLVEVRSMVNPPAAVKMALESICLLMGEPTSDWKAIRGIVIRDNFISTIVNFQTDDISDNIRETMVSKYLSEPKYNFETVDRASKACGPMVKWAIAQVNYADMLQRVDPLRQELRSLEEEADRNKMEGEEMNRLVQELEKSIARYKEEYAVLISQAEAIKASLLAVEEKVTRATDLLRSLGMERERWEAGSVSFKNQMATIVGDCLLSAAFLAYAGYFDQHMRQSLFNSWSHHLQQANVKFRPDIARVEYLSNADERLRWQANALPADDLCTENAIMLKRFNRYPLIIDPSGQATAFVMNEYRERTITKTSFLDDAFRKNLESALRFGNPLLVQDVESYDPILNPVLNREVRRTGGRVLITLGDQDIDLSPSFTIFLSTRDPTVEFPPDICSRVTFVNFTVTRSSLQSQCLNEVLKAERPDVDEKRSDLLKLQGEFHLRLRQLEKSLLQALNDVKGKILDDDRIITHLETLKKEAAEITRKVEETDAVMAEVDRVSQQYHPLAAACSSIYFTMDSTHQIHFLYQYSLQFFLDIYHAVLYENSHLQGVTDYMARLNVIIKDLFQESFKRVARGMLFKDRVTFAVLLVRIFLKGVVDEPTEYDAEFQHLLRGKETVVKSHELPIIEGLATEQLESMMRLTHLPSFSNLAVRVQAEDAFSSWVESVTPEVDVPVLWADDDTLTKTGRDMQRLLVIQAFRPDRLRAITHRFVQTVMGDSFMHCAEQELDLAATVENEIKSNTPLLLCSVPGYDASGHVEDVAAELGKGLTPIAIGSAEGFSLADKAINTATKSGRWVLLKNVHLAPQWLVQLEKKLHSLTPHRDFRLFLTMEINPKVPVNLLRAGRVFVFEPPPGVKANLLGTFSTIPAARMCKAPGERARLYFLLAWFHAVVQERLRYAPLGWSKCYEFNESDLRCSCDTLDTWIEMAAKGRANLPPDKVPWDALRILLSQAIYGGRVDNDFDQRLLNAFLDKLFTASSFERDFQLVSKVDGNKDIIMPEGIHRDQFLQWSEGLPDMQTPSWLGLPNNAEKVLLTTQGADMIINLLRMQMLEDDEDVMEEGRPRAGSRKRATSSGDGRPAWMRTLLMSASNWLSQIPKNLTVLRRTAENIKDPLFRFFEREVNAGSRLLKEVRTNLENVVLVCEGKKKQTNEDRELISVLAKGMLPQSWRRYKVPDGMTVIQWVIDFGQRIKQLHKISQATAQGGAKELKNQQIWLGGLFVPEAFITATRQYVSQANSWSLEELAMEVHISDGSGKDIPRDGGTFMVTGLKLQGAVCKQNTLQLSKTISTELPITCLRWIRYRHLFIFFISVTLPVYLNQTRADLLFTVDLQTAGSRSGANHGFYEHGVALLAAEWI

Target ORF information:

RefSeq Version	XM_002610666.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002610666.1

ORF Insert Sequence:

ATGCTGAAGA CGTTTGATCA CTACTGCGAG TACCGGAGGA CCCCCCACGG ACTGGTGCTG 
TCTCCCATCC AGCTGGGCAA GTGGCTGGTG GTCTTCTGTG ATGAGATCAA CCTGCCAGAC 
ATGGACAAGT ATGGCACGCA GCGTGTCATC TCCTTCCTGC GCCAGATGGT GGAGCACGGA 
GGCTTCTTCC GCTCAGCGGA CCAGCAGTGG GTCAAGTTCG AGAGGATCCA GTTTGTGGGA 
GCGTGCAATC CCCCAACGGA CCCAGGCCGC AAGCCTTTGT CTCACAGGTT CCTACGTCAC 
GTCCCTGTGG TGTATGTGGA CTACCCTGCA GCCCCCTCCC TCATGCAGAT CTACGGCACC 
TTCAACCGTG CCATGCTGCG CCTCATCCCC ACCCTGCGTA CCTACGCAGA GCCTCTCACT 
ATGGCAATGG TGGAGTTCTT CACTATGTCA CAGGAGAGGT TTACTCAGGA CACCCAGCCA 
CACTACATCT ACAGCCCACG TGAAATGACC CGCTGGGTGC GAGGCATCCT GGAGGCGCTG 
CGGCCCCTGG AGTCCCTCCC CGTGGAGGGG CTGGTGCGCA TCTGGGCCCA CGAGGGCCTC 
AGGCTCTTCC AGGACAGGCT GGTGGAAGAG GAGGAGCGTC AGTGGACAGA TGAGAACATC 
AACATGGTGG CGCTCAAACA CTTCCCCAAC ATCGACCAGC AGAAGGCTCT GGGTCGCCCC 
ATCCTATACA GCAAGTGGCT GTCAAAGGAC TACGTACCTG TGGACAGGGA GGAGCTGCGA 
GAGTACACCA AGGCTCGCCT CAAGGTGTTC TATGAAGAGG AGCTTGACGT GCCGCTGGTC 
CTGTTTAACG AGGTTCTGGA CAACGTCCTG CGCATCGACC GGATCTTCCA GCAGCCACAA 
GGTCACCTGC TGCTGATCGG AATGAGCGGG GCTGGCAAGA CGACCCTGTC GCGCTTCGTC 
GCCTGGATGA ACGGACTGAC TGTGTATCAG ATCAAGGTCC ACAGGAAGTA CATGGCAGCA 
GACTTTGATG AGGACCTGAG GAATGTGCTG AGACGGTCGG GCTGTAAGGA CGAGAAGATC 
GCCTTCATCA TGGATGAGTC CAACGTGCTG GACTCGGGCT TCCTGGAGAG GATCAACACT 
CTTCTGGCAA ACGGAGAGGT GCCAGGACTG TTTGAAGGAG ACGAGTACAC CACCCTGATG 
ACCCAGTGTA AGGAGGGTGC CCAGCGGAAC GGGCTGATGT TAGACACCGG CGAGGAGCTG 
TACAAGTGGT TCACCCAGCA GGTCATGAGG AACCTACACG TGGTCTTCAC CATGAACCCA 
TCCTCCCAGG GTCTGAAGGA CCGGGCTGCC ACGTCACCCG CCCTCTTCAA CAGATGTGTG 
CTGAACTGGT TCGGAGACTG GTCAGAGGGA GCCCTGTACC ACGTCGGCCT GGAGTTCACC 
AGCAAGTTGG ACCTGGAGCG GCCGGACTGG AAGCTGCCCG ACTACATCCC CCAGGCGTTC 
GAGGGGCTGC CTGACATGCC GACCCACAGG GACATCATCG CCAACGCCTT CGTCTACGTG 
CACCAGACGC TGCACCAGGC CAACCAGAGG CTGAGCAAAC GTGGCGCCCG CATCATGGCC 
ATCACACCTC GCCATTACCT GGACTTCATC AACCACTATG TGAAACTGTA CAACGAGAAG 
CGGTCTGACC TGGAGGAGCA GCAGCTCCAC CTGAACGTGG GCCTGTCCAA GATCCGGGAG 
ACTGTGGAGC AGGTGGAGGA GATGAGGAAG AGCCTGTCAG TCAAGAAGCA GGAGCTGGAG 
GCCAAGAACA ACCTGGCCAA CGCCAAGCTG AAGGAGATGG TGAAGGACCA GCAGGAGGCT 
GAGAGGAAGA AGGTGATGTC CATAGAAATC CAAGAGAAGC TGAAGACGCA GCTGGAACAG 
ATCAAGGTGA AGCGTGCGTA TGTGATGGAG GACCTGGCCA AGGTCGAGCC CGCTGTGATC 
GAGGCCCAGC AAGCTGTCAA GGGCATCAAG AAACAGCATC TGGTGGAGGT ACGTTCCATG 
GTGAACCCCC CTGCTGCAGT CAAGATGGCC CTGGAGTCCA TCTGCCTTCT AATGGGAGAA 
CCCACATCGG ACTGGAAGGC CATCCGTGGA ATTGTCATCA GGGACAACTT CATCAGCACC 
ATCGTCAACT TCCAGACTGA TGACATCTCA GACAACATTC GGGAGACCAT GGTGAGCAAG 
TACCTGTCCG AACCCAAGTA CAACTTTGAG ACTGTGGACA GGGCCAGCAA GGCCTGTGGG 
CCCATGGTCA AGTGGGCAAT TGCACAGGTA AACTATGCAG ACATGCTGCA GCGCGTGGAC 
CCGCTGCGAC AGGAGCTGCG CTCGCTGGAA GAGGAGGCCG ACAGGAACAA GATGGAGGGA 
GAGGAGATGA ACAGGCTGGT CCAGGAGCTC GAGAAGAGCA TCGCCAGGTA CAAGGAGGAG 
TATGCCGTGC TGATCAGCCA GGCGGAGGCC ATCAAGGCCA GCCTGCTGGC TGTGGAAGAG 
AAGGTGACCC GTGCCACGGA TCTCCTGAGG AGCCTGGGTA TGGAGAGGGA GCGATGGGAG 
GCCGGCAGCG TGTCCTTCAA GAACCAGATG GCCACCATCG TCGGAGACTG CCTGCTCTCC 
GCCGCCTTCC TGGCCTACGC GGGTTACTTC GACCAGCACA TGAGGCAGAG TCTGTTCAAC 
TCCTGGTCGC ACCACCTGCA GCAGGCCAAC GTGAAGTTCC GCCCGGACAT TGCCCGCGTG 
GAGTACCTCT CCAACGCCGA CGAGCGGCTG CGCTGGCAGG CCAACGCCCT CCCGGCCGAC 
GACCTCTGCA CAGAGAACGC CATCATGCTG AAGCGCTTCA ACCGCTACCC GCTGATCATC 
GACCCGTCCG GGCAGGCCAC GGCGTTCGTC ATGAACGAGT ACCGGGAGAG GACCATCACC 
AAGACCAGCT TCCTGGACGA CGCCTTCCGC AAGAACCTGG AGAGCGCGCT GCGCTTCGGG 
AACCCCCTGC TGGTGCAGGA CGTGGAGAGC TACGACCCCA TCCTCAATCC CGTGCTGAAC 
CGCGAGGTCC GACGCACCGG GGGCCGCGTG CTCATCACGC TGGGAGACCA GGACATTGAT 
CTGTCGCCTT CCTTCACCAT CTTCCTGTCC ACCCGGGATC CCACCGTGGA GTTCCCCCCG 
GACATCTGCT CCAGGGTGAC CTTCGTGAAC TTCACGGTGA CCCGCAGCAG TCTGCAGAGC 
CAGTGTCTGA ACGAGGTCCT GAAGGCAGAG CGGCCGGACG TCGACGAGAA GAGATCCGAC 
CTGCTGAAGC TGCAAGGTGA GTTCCACCTG CGTCTGCGCC AGCTGGAGAA GTCCCTGCTC 
CAGGCCCTGA ACGACGTGAA GGGCAAGATC CTGGACGACG ATCGCATCAT CACCCACCTG 
GAGACTCTGA AGAAGGAGGC CGCGGAGATC ACACGCAAGG TTGAGGAGAC AGATGCGGTG 
ATGGCTGAGG TGGATCGCGT GTCCCAGCAG TACCATCCCC TGGCAGCAGC CTGCAGCAGC 
ATCTACTTCA CCATGGACTC CACTCACCAG ATCCACTTCC TGTACCAGTA CTCCCTGCAG 
TTCTTCCTGG ATATCTACCA CGCAGTCCTG TATGAGAACT CCCACCTTCA GGGGGTGACA 
GACTACATGG CTCGCCTCAA CGTCATCATC AAGGACCTCT TCCAGGAGTC GTTCAAGCGC 
GTTGCCCGAG GCATGCTGTT CAAGGACAGG GTCACCTTTG CTGTGCTGCT GGTGCGCATC 
TTCCTGAAGG GAGTTGTGGA CGAGCCGACG GAGTACGATG CAGAGTTCCA GCACCTGCTG 
CGCGGGAAGG AGACGGTGGT GAAGAGCCAT GAGCTGCCAA TCATCGAGGG ACTGGCCACA 
GAACAGCTGG AGAGCATGAT GCGCCTGACC CACCTGCCCT CCTTCAGTAA CCTGGCCGTC 
CGTGTCCAGG CCGAGGACGC CTTCTCCTCC TGGGTGGAGT CGGTCACTCC GGAGGTGGAC 
GTTCCTGTGC TGTGGGCTGA TGACGACACG CTCACTAAGA CTGGGCGCGA CATGCAGCGA 
CTGCTGGTGA TCCAGGCCTT CCGTCCGGAC CGCCTGCGGG CCATCACCCA CAGGTTCGTG 
CAGACCGTGA TGGGAGACTC TTTCATGCAC TGTGCCGAGC AGGAGCTGGA CCTGGCTGCG 
ACTGTGGAGA ATGAGATCAA GTCCAACACC CCCCTCCTGC TGTGCTCTGT ACCCGGCTAC 
GATGCCAGCG GACATGTGGA GGATGTGGCA GCTGAGCTGG GCAAGGGTCT GACTCCCATT 
GCCATCGGGT CTGCTGAAGG ATTCAGCCTG GCCGACAAAG CCATCAACAC TGCCACCAAG 
TCTGGAAGGT GGGTGCTGTT GAAGAACGTC CATCTGGCTC CCCAGTGGCT GGTGCAGCTG 
GAGAAGAAGC TCCACTCACT CACACCGCAC CGCGACTTCC GCCTCTTCCT CACCATGGAG 
ATCAACCCCA AGGTGCCGGT GAACCTGCTG CGTGCCGGCC GTGTGTTTGT GTTTGAACCT 
CCTCCTGGTG TGAAGGCCAA CCTGCTGGGG ACCTTCAGCA CCATCCCTGC TGCACGCATG 
TGCAAGGCGC CTGGCGAGCG AGCACGGCTG TACTTCCTGC TGGCCTGGTT CCACGCTGTG 
GTGCAGGAGC GGCTCCGCTA CGCTCCCCTG GGATGGTCTA AGTGCTACGA GTTCAACGAG 
TCGGACCTCC GCTGTTCCTG CGATACGCTG GACACCTGGA TCGAAATGGC TGCAAAGGGT 
CGAGCTAACC TCCCACCAGA CAAGGTGCCG TGGGACGCCC TGCGTATACT GCTGTCCCAG 
GCCATCTATG GAGGGCGTGT TGACAATGAC TTTGATCAGC GCCTGTTGAA TGCCTTCCTG 
GACAAGCTGT TCACAGCCAG CAGCTTTGAG AGAGACTTCC AGCTGGTCAG CAAGGTGGAT 
GGGAACAAGG ACATCATCAT GCCTGAAGGC ATCCATCGGG ACCAGTTCCT GCAGTGGTCG 
GAGGGGCTGC CCGACATGCA GACTCCGTCC TGGCTAGGAC TGCCCAACAA TGCTGAGAAA 
GTGCTGCTCA CAACACAAGG GGCGGACATG ATCATCAACC TGCTGCGGAT GCAGATGCTG 
GAGGATGATG AGGATGTGAT GGAGGAGGGC CGACCCCGGG CCGGCAGCAG GAAGCGCGCC 
ACCAGCAGCG GGGACGGCCG CCCCGCCTGG ATGCGCACCC TGCTCATGTC TGCCTCCAAC 
TGGCTCTCAC AGATTCCTAA GAATTTGACA GTCCTTCGTC GAACAGCAGA AAACATCAAG 
GACCCTCTGT TCCGATTCTT CGAACGTGAG GTGAATGCTG GGTCTCGGCT GTTGAAGGAG 
GTCCGCACCA ACCTGGAGAA CGTGGTTCTG GTGTGTGAAG GCAAGAAGAA GCAGACCAAT 
GAAGACAGGG AGCTCATCAG TGTTCTGGCC AAAGGCATGC TGCCGCAGTC GTGGCGTCGG 
TACAAGGTTC CTGATGGCAT GACAGTTATC CAGTGGGTTA TAGACTTCGG CCAGCGTATC 
AAGCAGCTGC ACAAGATCTC CCAGGCCACA GCTCAGGGTG GGGCCAAGGA ACTTAAGAAC 
CAGCAGATCT GGCTGGGCGG TCTGTTTGTC CCCGAGGCCT TCATCACGGC CACCCGCCAG 
TACGTGTCGC AGGCCAACAG CTGGTCCCTG GAGGAGCTCG CAATGGAGGT GCACATCTCT 
GACGGCAGCG GCAAGGACAT CCCCAGGGAC GGCGGCACCT TCATGGTCAC AGGTTTGAAA 
CTCCAGGGAG CTGTCTGTAA GCAGAACACC CTGCAGCTGA GTAAGACCAT CTCTACTGAG 
CTGCCCATCA CCTGCCTCCG CTGGATCAGG TACAGACATT TATTCATTTT TTTTATTTCA 
GTGACCCTCC CGGTGTACCT GAACCAGACC CGTGCTGACC TGCTGTTCAC GGTGGACCTG 
CAGACCGCAG GCAGCCGTTC TGGCGCCAAC CATGGCTTCT ACGAGCATGG TGTGGCCCTG 
CTGGCAGCCG AGTGGATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_65941 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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