Amy cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	Amy
Entrez Gene ID	100379324
Full Name	alpha-amylase
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001173153.1	NP_001166624.1	alpha-amylase precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
Suetsugu Y, Futahashi R, Kanamori H, Kadono-Okuda K, Sasanuma S, Narukawa J, Ajimura M, Jouraku A, Namiki N, Shimomura M, Sezutsu H, Osanai-Futahashi M, Suzuki MG, Daimon T, Shinoda T, Taniai K, Asaoka K, Niwa R, Kawaoka S, Katsuma S, Tamura T, Noda H, Kasahara M, Sugano S, Suzuki Y, Fujiwara H, Kataoka H, Arunkumar KP, Tomar A, Nagaraju J, Goldsmith MR, Feng Q, Xia Q, Yamamoto K, Shimada T, Mita K
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)

Cloning and expression analysis of the Bombyx mori α-amylase gene (Amy) from the indigenous Thai silkworm strain, Nanglai.
Ngernyuang N, Kobayashi I, Promboon A, Ratanapo S, Tamura T, Ngernsiri L
Journal of insect science (Online)1138(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Amy gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Amy cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBg01418	NM_001173153.1 Latest version!	Bombyx mori alpha-amylase (Amy), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Amy ( NM_001173153.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001166624.1 Bombyx mori alpha-amylase (Amy), mRNA.

Price & Availability↑

CloneID	OBg01418
Clone ID Related Accession (Same CDS sequence)	NM_001173153.1
Accession Version	NM_001173153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556812800000
Organism	Bombyx mori(domestic silkworm)
Product	alpha-amylase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ274006.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03699706 [ECO:0000348] ##Evidence-Data-END##

ATGTTTCGGT ACATCCTTCT ACTTTCGGCC GTGACTCTGG CGTTGGCCTA TAAAAACCCT 
CACTATGCAT CGGGTCGCAC CACCATGGTA CATCTCTTCG AATGGAAGTG GGATGACATC 
GCTGCTGAAT GCGAAAGGTT CCTTGGACCC CGAGGATTCG GTGGTATTCA GGTTTCGCCA 
CCAAACGAGA ATTTGGTAAT CTGGTCCCGC AACCGTCCTT GGTGGGAGCG CTATCAACCA 
ATCTCCTACC GTCTAGTAAC AAGATCTGGA AATGAAAATC AATTTTCGAA TATGGTGCGT 
CGCTGCAACA ATGTTGGCGT CAGGATTTAT GTGGACGCCA TCATCAACCA CATGACTGGA 
ACTTGGAATG AGAATGTTGG TACAGGTGGA AGCACGGCCA ATTTCGAAAA CTGGCACTAT 
CCTGCTGTTC CTTACGGCAG GAATGACTTC AACTGGCCTC ATTGTGTCAT TACTGGCAGC 
GACTACAACT GCTGCCCTGA CAGAGTGCGC AACTGTGAAT TGTCTGGTCT GAAAGACTTG 
AACCAAGGCT CTGATTATGT TAGACAGCAG ATTCTAAATT ATATGAACCG TCTTATTGAC 
ATGGGTGTTG CTGGTTTCAG AATTGATGCT GCAAAGCACA TGTGGCCTCA CGATTTGCGA 
GTGATCTACG ATCGTCTCCG CAATTTAAAT ACCGCCCACG GTTTCCCATC CGGTGCTCGT 
CCCTACATCT ACCAAGAAGT CATCGACCTC GGTGGTGAAG CTATCAGTCG CAACGAATAC 
ACTCCACTTG CTGCCGTTAC TGAGTTCAGA TTTGGACTGG AACTCAGTCA AGCTTTCCAA 
AGAAGAAACC AGCTTAGATG GTTGGTTAAC TGGGGTCCAC AGTGGGGCCT TCTCGCTTCT 
GGAGACGCTT TGACTTTCAT TGACAACCAC GACAACCAGA GAGGTCATGG CGCTGGTGGA 
AACATCTTGA CATACAAGCA ATCCCGACAA TACAAGGGCG CTATCGCTTT CATGTTGGCA 
CATCCTTATG GCTACCCTCA ATTGATGAGT AGTTTCGCCT TCACGGACAC CGAAGCTGGA 
CCTCCAATGA ACAGCAGGGG TGATATCACC TCTCCTACTA TCAACGCTGA TAATTCTTGC 
GGCAATGGTT GGATATGCGA GCACCGTTGG CGTCAGATCC ACAACATGGT TGTCTTCAGG 
AACACCGCTG GTAACGGTGC CCTTACCAAC TGGTGGGACA ACGGTAGCAA CCAGATTGCT 
TTCTGCCGCG GCAACCAGGC CTTCATTGCT TTCAACAACG ATGCATGGGA CATGGACCAG 
ACTCTTCAGA CTTGTCTCCC CGCCGGAACA TACTGCGATA TTATTTCCGG CGCCAGGTCT 
GGTAACCGCT GCACTGGAAA ATCTATTGTA GTAGGCAGCG ATGGTCGCGC TCTTATCATC 
CACCGCAGCA ACGAGTATGA CATGATGGTT GCTATCCATA GGGGTGCTGA CTCGAGATTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166624.1
CDS	55..1557
Translation	MFRYILLLSAVTLALAYKNPHYASGRTTMVHLFEWKWDDIAAECERFLGPRGFGGIQVSPPNENLVIWSRNRPWWERYQPISYRLVTRSGNENQFSNMVRRCNNVGVRIYVDAIINHMTGTWNENVGTGGSTANFENWHYPAVPYGRNDFNWPHCVITGSDYNCCPDRVRNCELSGLKDLNQGSDYVRQQILNYMNRLIDMGVAGFRIDAAKHMWPHDLRVIYDRLRNLNTAHGFPSGARPYIYQEVIDLGGEAISRNEYTPLAAVTEFRFGLELSQAFQRRNQLRWLVNWGPQWGLLASGDALTFIDNHDNQRGHGAGGNILTYKQSRQYKGAIAFMLAHPYGYPQLMSSFAFTDTEAGPPMNSRGDITSPTINADNSCGNGWICEHRWRQIHNMVVFRNTAGNGALTNWWDNGSNQIAFCRGNQAFIAFNNDAWDMDQTLQTCLPAGTYCDIISGARSGNRCTGKSIVVGSDGRALIIHRSNEYDMMVAIHRGADSRL

Target ORF information:

RefSeq Version	NM_001173153.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori alpha-amylase (Amy), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173153.1

ORF Insert Sequence:

ATGTTTCGGT ACATCCTTCT ACTTTCGGCC GTGACTCTGG CGTTGGCCTA TAAAAACCCT 
CACTATGCAT CGGGTCGCAC CACCATGGTA CATCTCTTCG AATGGAAGTG GGATGACATC 
GCTGCTGAAT GCGAAAGGTT CCTTGGACCC CGAGGATTCG GTGGTATTCA GGTTTCGCCA 
CCAAACGAGA ATTTGGTAAT CTGGTCCCGC AACCGTCCTT GGTGGGAGCG CTATCAACCA 
ATCTCCTACC GTCTAGTAAC AAGATCTGGA AATGAAAATC AATTTTCGAA TATGGTGCGT 
CGCTGCAACA ATGTTGGCGT CAGGATTTAT GTGGACGCCA TCATCAACCA CATGACTGGA 
ACTTGGAATG AGAATGTTGG TACAGGTGGA AGCACGGCCA ATTTCGAAAA CTGGCACTAT 
CCTGCTGTTC CTTACGGCAG GAATGACTTC AACTGGCCTC ATTGTGTCAT TACTGGCAGC 
GACTACAACT GCTGCCCTGA CAGAGTGCGC AACTGTGAAT TGTCTGGTCT GAAAGACTTG 
AACCAAGGCT CTGATTATGT TAGACAGCAG ATTCTAAATT ATATGAACCG TCTTATTGAC 
ATGGGTGTTG CTGGTTTCAG AATTGATGCT GCAAAGCACA TGTGGCCTCA CGATTTGCGA 
GTGATCTACG ATCGTCTCCG CAATTTAAAT ACCGCCCACG GTTTCCCATC CGGTGCTCGT 
CCCTACATCT ACCAAGAAGT CATCGACCTC GGTGGTGAAG CTATCAGTCG CAACGAATAC 
ACTCCACTTG CTGCCGTTAC TGAGTTCAGA TTTGGACTGG AACTCAGTCA AGCTTTCCAA 
AGAAGAAACC AGCTTAGATG GTTGGTTAAC TGGGGTCCAC AGTGGGGCCT TCTCGCTTCT 
GGAGACGCTT TGACTTTCAT TGACAACCAC GACAACCAGA GAGGTCATGG CGCTGGTGGA 
AACATCTTGA CATACAAGCA ATCCCGACAA TACAAGGGCG CTATCGCTTT CATGTTGGCA 
CATCCTTATG GCTACCCTCA ATTGATGAGT AGTTTCGCCT TCACGGACAC CGAAGCTGGA 
CCTCCAATGA ACAGCAGGGG TGATATCACC TCTCCTACTA TCAACGCTGA TAATTCTTGC 
GGCAATGGTT GGATATGCGA GCACCGTTGG CGTCAGATCC ACAACATGGT TGTCTTCAGG 
AACACCGCTG GTAACGGTGC CCTTACCAAC TGGTGGGACA ACGGTAGCAA CCAGATTGCT 
TTCTGCCGCG GCAACCAGGC CTTCATTGCT TTCAACAACG ATGCATGGGA CATGGACCAG 
ACTCTTCAGA CTTGTCTCCC CGCCGGAACA TACTGCGATA TTATTTCCGG CGCCAGGTCT 
GGTAACCGCT GCACTGGAAA ATCTATTGTA GTAGGCAGCG ATGGTCGCGC TCTTATCATC 
CACCGCAGCA ACGAGTATGA CATGATGGTT GCTATCCATA GGGGTGCTGA CTCGAGATTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
G3 (Bethesda, Md.)3(9)1481-92(2013 Sep)
Suetsugu Y,Futahashi R,Kanamori H,Kadono-Okuda K,Sasanuma S,Narukawa J,Ajimura M,Jouraku A,Namiki N,Shimomura M,Sezutsu H,Osanai-Futahashi M,Suzuki MG,Daimon T,Shinoda T,Taniai K,Asaoka K,Niwa R,Kawaoka S,Katsuma S,Tamura T,Noda H,Kasahara M,Sugano S,Suzuki Y,Fujiwara H,Kataoka H,Arunkumar KP,Tomar A,Nagaraju J,Goldsmith MR,Feng Q,Xia Q,Yamamoto K,Shimada T,Mita K

Cloning and expression analysis of the Bombyx mori α-amylase gene (Amy) from the indigenous Thai silkworm strain, Nanglai.
Journal of insect science (Online)1138(2011)
Ngernyuang N,Kobayashi I,Promboon A,Ratanapo S,Tamura T,Ngernsiri L

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Amy cDNA ORF clone, Bombyx mori(domestic silkworm)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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