BDBG_02382 cDNA ORF clone, Blastomyces gilchristii SLH14081

Gene
Clones
gRNAs

Summary

Gene Symbol	BDBG_02382
Entrez Gene ID	8510124
Full Name	serine/threonine protein kinase
Gene Type	protein-coding
Organism	Blastomyces gilchristii SLH14081

mRNA and Protein(s)

mRNA	Protein	Name
XM_031720762.1	XP_031577038.1	PLK protein kinase
XM_002627665.1	XP_002627711.1	serine/threonine protein kinase
XM_031720763.1	XP_031577039.1	PLK protein kinase, variant

Pathways from BioSystems
Gene Ontology

The following BDBG_02382 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BDBG_02382 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAl08774	XM_002627665.1 Latest version!	Ajellomyces dermatitidis SLH14081 serine/threonine protein kinase, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAl116117	XM_031720763.1 Latest version!	Blastomyces gilchristii SLH14081 PLK protein kinase, variant (BDBG_02382), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAl116117	XM_031720762.1 Latest version!	Blastomyces gilchristii SLH14081 PLK protein kinase (BDBG_02382), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BDBG_02382 ( XM_002627665.1 ) cDNA ORF clone, Blastomyces gilchristii SLH14081 -> XP_002627711.1 Ajellomyces dermatitidis SLH14081 serine/threonine protein kinase, mRNA.

Price & Availability↑

CloneID	OAl08774
Clone ID Related Accession (Same CDS sequence)	XM_002627665.1
Accession Version	XM_002627665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254931200000
Organism	Blastomyces gilchristii SLH14081
Product	serine/threonine protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101680). COMPLETENESS: incomplete on both ends.

ATGGAGGCAT TGTCACCTCG CTCAACCAAC ATTCTCTCCA AGCCCAAGAC CGACCCTATA 
ATGAAGGCGA GCGCCAAAGA TGCACCGCCT CAAAAGGCGC GGTCCGCCAA AAACCACGCC 
CCCCCACCTC CTGCTACGGT TCGTGAGCCT GAAGATGGGG GGGAGGAATA CATCACTGGC 
AAATTTTTAG GAAAAGGGGG ATTTGCCGTA TGTTATGAAG GGAGGCTTGC GCGAAATAAC 
AGGGTATATG CGATGAAAGT GGTAAAATCG GAAATGAACC AGAGGAAAAT GGAGGAGAAG 
TTCCGGACCG AGCTCCAGAT ACACTCAAAA ATGCGCCATC CCAACATTGT TGGTTTCTAC 
AGGGCATTTG CCTTCGAACA AAACACTTAT GTCATTTTGG AATTGTGCCC CAATGGCTCG 
GTGTCGGATA TGGTGAGGAA GCGGAAATAC CTGAGCTTGC CCGAAGTCAG GAGATTTATG 
ATTCAGCTTT GCGGGGCGGT TAAATATATG CATAAGAGAA ACGTGGCCCA TCGAGACCTG 
AAAATGGGGA ATCTGTTTCT CGACCGGAAT ATGGACATCA AAGTTGGAGA CTTTGGCCTA 
GCTGCTATTA TCCTGTCGGA GAAGGACGAA AAGCGACGGA GAACACTATG TGGAACTCCA 
AATTATATTG CGCCGGAAGT TTTGGATAAG AGCAAGGGAG GCCATAATCA AAAGGTTGAC 
ATATGGTCGC TTGGCGTAAT TTTCTCCTGC CTGAATTTGA ACGAGGATGA ACGACCGGAG 
CCCGACCAGA TTGTTGATCA CGCATTTTTC AATATGTACA ATGGCTGTAT ACCACGTCAA 
CTGGACCCAG CCACACGCCT CGCTCCACCT ACCTGGCTGA CAACCCAAGA CCCTCGAGGA 
GACCGTATGA GTCACGGATA TAGTCTGGAC CATGACTCGA AGTATTTGGG GAAAATAGCC 
CATGCGAAAA ACTCCAATGA GCGATATCTT ATTTGCAAAA ACGAGTTTTA CACAGAATGT 
GGGGTTGGTA GGACTTCTAC TGGATCAATT AGGAGGCCAG CAGGGAAACG GTGTTCCAAA 
TCTGCCTTCG CTGAATGTGC TGCTGAAGAA GAAAGGGGGA TGCAGCCCAT CGTCCCCATA 
CCAGAAGATC TAGTCTACAG CTATTACACC GGATCCAATG GAGATTGGAG CCATACAACG 
GAAGAGGATG ATGCTCGGCC CGATCCTGAT AGTCCCATTG AGGGTGAAGT GCTTCCCAAA 
ACGCGTAACG CATCGTATAA ACAGCAAGCC GCCACAGTTG CTCGCATAAC ATCGGCTTTG 
GCTGCACAGA TTCGCCGCAA AGAAGCTCAA ACCCAAAGCC ATGCAGCTCT CCTACGGCAG 
CAAGCTGTGC CACAACGACA GCCAACAATG AACCCCTCGT CTTCCCAACA CCCTCAAGGT 
GATATTAAAG ACACCGCAGA AAATGGCATT CAGACCGAGA CCATTCCTCC CCAGCGGCTG 
CTGAATGAGC GACCAATCCG GGCACGGAGG GTAGTGTCTG GTTATTCTGC ATCTGTCCGC 
GACAGGAACA TATCGTTGAC TTCCGCCTTG CCTAAATCCA ATTCGGAACC AATTAATTTG 
ACCATCGGAA AAACGAGATC ACAGTCGAGG CTACAAATGG CAGCGTTGAC GCGAGAGAGA 
AACGAATTAG CACTGGAAGC CAATGAAGTG AACCCGCTAG ATATCAGAAA TAAAGACATG 
TTTGCTTCGG TTCGCGGGAT GCCTGCGAGA ACAGTTAGAG TCGACCCGCG CGAGACAGAA 
AGACAGCGAC CGTGGGAACA GCCAGTGAAC TCACAGCAAC AACAATCTCG TGATGTGAAA 
CTGGATGAGA GGTCCAGGAG CGCTAACAGT AGTGGAAGTA ATGGCAGCAA ATCCACCAGC 
TCTTCTAACA AATCACGATC CACACTTGGG CTTAGTCCTC TAATACATCC CGACGAACAT 
GCAGAACTTA TGCCGGGGAC GACCGCCAGT GAAGTTTTGA CTGATTTGAG AACATACCTT 
AGAAGCTTTG GGCATCTGGA ATCATTGACA GCATCGACTT CAAGGACACG GCAACGGCAA 
CAATTTTCAT CACTCTCCGA TCCACATCCC TATGTCGTCA AGTGGGTGGA CTATACGAAT 
AGGTACGGTA TCGGGTACGT TTTGGACGAC GGAAGCGTGG GCTGCGTTTT CAAAGCAGAG 
CACGGCCGCC CAGCCAGCTG CGTTGTGGTC CGGAATGGAG AACGGCATAT CCGTCGGAAA 
GCCTATGCGA AAGAAAATCC TCGTAGTGCA TATGCGGATG CCGATCAACT CGTGCCAAGG 
AATGGTCAAC CTGTGGAATT TTATGAAAAT GTTGATGTAC AAGACAATTC ATACTCTGGC 
GGTGGAACGA GAAGGGTGCT TGTTCAGCCG GATGCATTTG AAGTGAGGAA TCAAAATGGC 
CCCGGTGGGC CCGGCATAAA AGTTCGGATG AATTCTGGAG TTGACTGTGC AAGGTGTGAT 
GCGGAGAAAG TCAAACGAGT CAAGCTTGTC GACCAGTTCG GCAAATATAT GATTGGAACA 
TTGGGAAGGA GTGTGGATGA AAACTGCCCT CCCGAGGATG AGAGAAACTA TAGATTTTCC 
GGCAAAATCG GTCAATATGT CAAATTCTAT CAGCGACTAG GGAATGTTGG CATATGGGGT 
TTTGGGGATG GAGCGTTCCA GTTCAATTTT CCGGATCATA CTAAACTTGT TATTTCACTG 
CCACGACATG GATCGCAAGG CTCGTCGGAG CCACAGCCTT GCCAGGCAGA TTTTTATCAT 
CTTTCTCCAT CTGCGGCACG CTACCTATCA ACCAGGGGCA GAATGCATCC AGCAGGTTTT 
GATACCAGAG CCGTTATATC TGAAAACCTG AACACCTATC TCGCCGCACT CTCAGGTCAT 
CCTATCCACC CATCCGGAAT TAGCAGCAGT CGGTTTTGCG ATATATTAGA GGCGAATGCG 
TTCCGCGACA AATTAGACTT CGTTCAACAG GTTCTGCGGA GCTGGTTTGA GAGCAGGAGG 
TTAGGTAACA GAGTACTGCT ACGGAATACA TCATCGACGA CCATTAATAC TGGTCCAGAT 
AAAACCATCC CTGACAACTT AACATCCAAA CGAGGCTACC GCGGCGAAAT ATTTTGGGAC 
GGTGCTCAGG AGAAATCCTG GTTGACGCCG TCTGGGGGTA AATTTGTGTG GGTTACTGTT 
GGCGCCCATG GCGGCGACGG CGAGTATTTT GCCGTACAGC TCAAGGGAAA TCCTGAAGGC 
GGAACAGTCG AGTGCGTTGG CGCTGAACAG ATACAAGATC TGCAAGATCA ATTGATGGCG 
TTAACTGTGC GGTCGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002627711.1
CDS	1..3381
Translation	MEALSPRSTNILSKPKTDPIMKASAKDAPPQKARSAKNHAPPPPATVREPEDGGEEYITGKFLGKGGFAVCYEGRLARNNRVYAMKVVKSEMNQRKMEEKFRTELQIHSKMRHPNIVGFYRAFAFEQNTYVILELCPNGSVSDMVRKRKYLSLPEVRRFMIQLCGAVKYMHKRNVAHRDLKMGNLFLDRNMDIKVGDFGLAAIILSEKDEKRRRTLCGTPNYIAPEVLDKSKGGHNQKVDIWSLGVIFSCLNLNEDERPEPDQIVDHAFFNMYNGCIPRQLDPATRLAPPTWLTTQDPRGDRMSHGYSLDHDSKYLGKIAHAKNSNERYLICKNEFYTECGVGRTSTGSIRRPAGKRCSKSAFAECAAEEERGMQPIVPIPEDLVYSYYTGSNGDWSHTTEEDDARPDPDSPIEGEVLPKTRNASYKQQAATVARITSALAAQIRRKEAQTQSHAALLRQQAVPQRQPTMNPSSSQHPQGDIKDTAENGIQTETIPPQRLLNERPIRARRVVSGYSASVRDRNISLTSALPKSNSEPINLTIGKTRSQSRLQMAALTRERNELALEANEVNPLDIRNKDMFASVRGMPARTVRVDPRETERQRPWEQPVNSQQQQSRDVKLDERSRSANSSGSNGSKSTSSSNKSRSTLGLSPLIHPDEHAELMPGTTASEVLTDLRTYLRSFGHLESLTASTSRTRQRQQFSSLSDPHPYVVKWVDYTNRYGIGYVLDDGSVGCVFKAEHGRPASCVVVRNGERHIRRKAYAKENPRSAYADADQLVPRNGQPVEFYENVDVQDNSYSGGGTRRVLVQPDAFEVRNQNGPGGPGIKVRMNSGVDCARCDAEKVKRVKLVDQFGKYMIGTLGRSVDENCPPEDERNYRFSGKIGQYVKFYQRLGNVGIWGFGDGAFQFNFPDHTKLVISLPRHGSQGSSEPQPCQADFYHLSPSAARYLSTRGRMHPAGFDTRAVISENLNTYLAALSGHPIHPSGISSSRFCDILEANAFRDKLDFVQQVLRSWFESRRLGNRVLLRNTSSTTINTGPDKTIPDNLTSKRGYRGEIFWDGAQEKSWLTPSGGKFVWVTVGAHGGDGEYFAVQLKGNPEGGTVECVGAEQIQDLQDQLMALTVRSG

Target ORF information:

RefSeq Version	XM_002627665.1
Organism	Blastomyces gilchristii SLH14081
Definition	Ajellomyces dermatitidis SLH14081 serine/threonine protein kinase, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002627665.1

ORF Insert Sequence:

ATGGAGGCAT TGTCACCTCG CTCAACCAAC ATTCTCTCCA AGCCCAAGAC CGACCCTATA 
ATGAAGGCGA GCGCCAAAGA TGCACCGCCT CAAAAGGCGC GGTCCGCCAA AAACCACGCC 
CCCCCACCTC CTGCTACGGT TCGTGAGCCT GAAGATGGGG GGGAGGAATA CATCACTGGC 
AAATTTTTAG GAAAAGGGGG ATTTGCCGTA TGTTATGAAG GGAGGCTTGC GCGAAATAAC 
AGGGTATATG CGATGAAAGT GGTAAAATCG GAAATGAACC AGAGGAAAAT GGAGGAGAAG 
TTCCGGACCG AGCTCCAGAT ACACTCAAAA ATGCGCCATC CCAACATTGT TGGTTTCTAC 
AGGGCATTTG CCTTCGAACA AAACACTTAT GTCATTTTGG AATTGTGCCC CAATGGCTCG 
GTGTCGGATA TGGTGAGGAA GCGGAAATAC CTGAGCTTGC CCGAAGTCAG GAGATTTATG 
ATTCAGCTTT GCGGGGCGGT TAAATATATG CATAAGAGAA ACGTGGCCCA TCGAGACCTG 
AAAATGGGGA ATCTGTTTCT CGACCGGAAT ATGGACATCA AAGTTGGAGA CTTTGGCCTA 
GCTGCTATTA TCCTGTCGGA GAAGGACGAA AAGCGACGGA GAACACTATG TGGAACTCCA 
AATTATATTG CGCCGGAAGT TTTGGATAAG AGCAAGGGAG GCCATAATCA AAAGGTTGAC 
ATATGGTCGC TTGGCGTAAT TTTCTCCTGC CTGAATTTGA ACGAGGATGA ACGACCGGAG 
CCCGACCAGA TTGTTGATCA CGCATTTTTC AATATGTACA ATGGCTGTAT ACCACGTCAA 
CTGGACCCAG CCACACGCCT CGCTCCACCT ACCTGGCTGA CAACCCAAGA CCCTCGAGGA 
GACCGTATGA GTCACGGATA TAGTCTGGAC CATGACTCGA AGTATTTGGG GAAAATAGCC 
CATGCGAAAA ACTCCAATGA GCGATATCTT ATTTGCAAAA ACGAGTTTTA CACAGAATGT 
GGGGTTGGTA GGACTTCTAC TGGATCAATT AGGAGGCCAG CAGGGAAACG GTGTTCCAAA 
TCTGCCTTCG CTGAATGTGC TGCTGAAGAA GAAAGGGGGA TGCAGCCCAT CGTCCCCATA 
CCAGAAGATC TAGTCTACAG CTATTACACC GGATCCAATG GAGATTGGAG CCATACAACG 
GAAGAGGATG ATGCTCGGCC CGATCCTGAT AGTCCCATTG AGGGTGAAGT GCTTCCCAAA 
ACGCGTAACG CATCGTATAA ACAGCAAGCC GCCACAGTTG CTCGCATAAC ATCGGCTTTG 
GCTGCACAGA TTCGCCGCAA AGAAGCTCAA ACCCAAAGCC ATGCAGCTCT CCTACGGCAG 
CAAGCTGTGC CACAACGACA GCCAACAATG AACCCCTCGT CTTCCCAACA CCCTCAAGGT 
GATATTAAAG ACACCGCAGA AAATGGCATT CAGACCGAGA CCATTCCTCC CCAGCGGCTG 
CTGAATGAGC GACCAATCCG GGCACGGAGG GTAGTGTCTG GTTATTCTGC ATCTGTCCGC 
GACAGGAACA TATCGTTGAC TTCCGCCTTG CCTAAATCCA ATTCGGAACC AATTAATTTG 
ACCATCGGAA AAACGAGATC ACAGTCGAGG CTACAAATGG CAGCGTTGAC GCGAGAGAGA 
AACGAATTAG CACTGGAAGC CAATGAAGTG AACCCGCTAG ATATCAGAAA TAAAGACATG 
TTTGCTTCGG TTCGCGGGAT GCCTGCGAGA ACAGTTAGAG TCGACCCGCG CGAGACAGAA 
AGACAGCGAC CGTGGGAACA GCCAGTGAAC TCACAGCAAC AACAATCTCG TGATGTGAAA 
CTGGATGAGA GGTCCAGGAG CGCTAACAGT AGTGGAAGTA ATGGCAGCAA ATCCACCAGC 
TCTTCTAACA AATCACGATC CACACTTGGG CTTAGTCCTC TAATACATCC CGACGAACAT 
GCAGAACTTA TGCCGGGGAC GACCGCCAGT GAAGTTTTGA CTGATTTGAG AACATACCTT 
AGAAGCTTTG GGCATCTGGA ATCATTGACA GCATCGACTT CAAGGACACG GCAACGGCAA 
CAATTTTCAT CACTCTCCGA TCCACATCCC TATGTCGTCA AGTGGGTGGA CTATACGAAT 
AGGTACGGTA TCGGGTACGT TTTGGACGAC GGAAGCGTGG GCTGCGTTTT CAAAGCAGAG 
CACGGCCGCC CAGCCAGCTG CGTTGTGGTC CGGAATGGAG AACGGCATAT CCGTCGGAAA 
GCCTATGCGA AAGAAAATCC TCGTAGTGCA TATGCGGATG CCGATCAACT CGTGCCAAGG 
AATGGTCAAC CTGTGGAATT TTATGAAAAT GTTGATGTAC AAGACAATTC ATACTCTGGC 
GGTGGAACGA GAAGGGTGCT TGTTCAGCCG GATGCATTTG AAGTGAGGAA TCAAAATGGC 
CCCGGTGGGC CCGGCATAAA AGTTCGGATG AATTCTGGAG TTGACTGTGC AAGGTGTGAT 
GCGGAGAAAG TCAAACGAGT CAAGCTTGTC GACCAGTTCG GCAAATATAT GATTGGAACA 
TTGGGAAGGA GTGTGGATGA AAACTGCCCT CCCGAGGATG AGAGAAACTA TAGATTTTCC 
GGCAAAATCG GTCAATATGT CAAATTCTAT CAGCGACTAG GGAATGTTGG CATATGGGGT 
TTTGGGGATG GAGCGTTCCA GTTCAATTTT CCGGATCATA CTAAACTTGT TATTTCACTG 
CCACGACATG GATCGCAAGG CTCGTCGGAG CCACAGCCTT GCCAGGCAGA TTTTTATCAT 
CTTTCTCCAT CTGCGGCACG CTACCTATCA ACCAGGGGCA GAATGCATCC AGCAGGTTTT 
GATACCAGAG CCGTTATATC TGAAAACCTG AACACCTATC TCGCCGCACT CTCAGGTCAT 
CCTATCCACC CATCCGGAAT TAGCAGCAGT CGGTTTTGCG ATATATTAGA GGCGAATGCG 
TTCCGCGACA AATTAGACTT CGTTCAACAG GTTCTGCGGA GCTGGTTTGA GAGCAGGAGG 
TTAGGTAACA GAGTACTGCT ACGGAATACA TCATCGACGA CCATTAATAC TGGTCCAGAT 
AAAACCATCC CTGACAACTT AACATCCAAA CGAGGCTACC GCGGCGAAAT ATTTTGGGAC 
GGTGCTCAGG AGAAATCCTG GTTGACGCCG TCTGGGGGTA AATTTGTGTG GGTTACTGTT 
GGCGCCCATG GCGGCGACGG CGAGTATTTT GCCGTACAGC TCAAGGGAAA TCCTGAAGGC 
GGAACAGTCG AGTGCGTTGG CGCTGAACAG ATACAAGATC TGCAAGATCA ATTGATGGCG 
TTAACTGTGC GGTCGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BDBG_02382 ( XM_031720762.1 ) cDNA ORF clone, Blastomyces gilchristii SLH14081 -> XP_031577038.1 Blastomyces gilchristii SLH14081 PLK protein kinase (BDBG_02382), mRNA.

Price & Availability↑

CloneID	OAl116117
Clone ID Related Accession (Same CDS sequence)	XM_031720762.1 , XM_031720763.1
Accession Version	XM_031720762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574697600000
Organism	Blastomyces gilchristii SLH14081
Product	PLK protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101680).

ATGGAGGCAT TGTCACCTCG CTCAACCAAC ATTCTCTCCA AGCCCAAGAC CGACCCTATA 
ATGAAGGCGA GCGCCAAAGA TGCACCGCCT CAAAAGGCGC GGTCCGCCAA AAACCACGCC 
CCCCCACCTC CTGCTACGGT TCGTGAGCCT GAAGATGGGG GGGAGGAATA CATCACTGGC 
AAATTTTTAG GAAAAGGGGG ATTTGCCGTA TGTTATGAAG GGAGGCTTGC GCGAAATAAC 
AGGGTATATG CGATGAAAGT GGTAAAATCG GAAATGAACC AGAGGAAAAT GGAGGAGAAG 
TTCCGGACCG AGCTCCAGAT ACACTCAAAA ATGCGCCATC CCAACATTGT TGGTTTCTAC 
AGGGCATTTG CCTTCGAACA AAACACTTAT GTCATTTTGG AATTGTGCCC CAATGGCTCG 
GTGTCGGATA TGGTGAGGAA GCGGAAATAC CTGAGCTTGC CCGAAGTCAG GAGATTTATG 
ATTCAGCTTT GCGGGGCGGT TAAATATATG CATAAGAGAA ACGTGGCCCA TCGAGACCTG 
AAAATGGGGA ATCTGTTTCT CGACCGGAAT ATGGACATCA AAGTTGGAGA CTTTGGCCTA 
GCTGCTATTA TCCTGTCGGA GAAGGACGAA AAGCGACGGA GAACACTATG TGGAACTCCA 
AATTATATTG CGCCGGAAGT TTTGGATAAG AGCAAGGGAG GCCATAATCA AAAGGTTGAC 
ATATGGTCGC TTGGCGTAAT TTTTTTTGCC ATGCTCACGG GATACCCCCC ATTTCAATCG 
AAGACGCAGG ACGAAATCTA CAAGAAAGTC AAAAATCTTA CATATGTCTG GCCCAAGGAG 
TCCGAGGGTG CTAATTACAT TCCAGTGGAG GCAAAGCACT TGGTCAGCTC CTGCCTGAAT 
TTGAACGAGG ATGAACGACC GGAGCCCGAC CAGATTGTTG ATCACGCATT TTTCAATATG 
TACAATGGCT GTATACCACG TCAACTGGAC CCAGCCACAC GCCTCGCTCC ACCTACCTGG 
CTGACAACCC AAGACCCTCG AGGAGACCGT ATGAGTCACG GATATAGTCT GGACCATGAC 
TCGAAGTATT TGGGGAAAAT AGCCCATGCG AAAAACTCCA ATGAGCGATA TCTTATTTGC 
AAAAACGAGT TTTACACAGA ATGTGGGGTT GGTAGGACTT CTACTGGATC AATTAGGAGG 
CCAGCAGGGA AACGGTGTTC CAAATCTGCC TTCGCTGAAT GTGCTGCTGA AGAAGAAAGG 
GGGATGCAGC CCATCGTCCC CATACCAGAA GATCTAGTCT ACAGCTATTA CACCGGATCC 
AATGGAGATT GGAGCCATAC AACGGAAGAG GATGATGCTC GGCCCGATCC TGATAGTCCC 
ATTGAGGGTG AAGTGCTTCC CAAAACGCGT AACGCATCGT ATAAACAGCA AGCCGCCACA 
GTTGCTCGCA TAACATCGGC TTTGGCTGCA CAGATTCGCC GCAAAGAAGC TCAAACCCAA 
AGCCATGCAG CTCTCCTACG GCAGCAAGCT GTGCCACAAC GACAGCCAAC AATGAACCCC 
TCGTCTTCCC AACACCCTCA AGGTGATATT AAAGACACCG CAGAAAATGG CATTCAGACC 
GAGACCATTC CTCCCCAGCG GCTGCTGAAT GAGCGACCAA TCCGGGCACG GAGGGTAGTG 
TCTGGTTATT CTGCATCTGT CCGCGACAGG AACATATCGT TGACTTCCGC CTTGCCTAAA 
TCCAATTCGG AACCAATTAA TTTGACCATC GGAAAAACGA GATCACAGTC GAGGCTACAA 
ATGGCAGCGT TGACGCGAGA GAGAAACGAA TTAGCACTGG AAGCCAATGA AGTGAACCCG 
CTAGATATCA GAAATAAAGA CATGTTTGCT TCGGTTCGCG GGATGCCTGC GAGAACAGTT 
AGAGTCGACC CGCGCGAGAC AGAAAGACAG CGACCGTGGG AACAGCCAGT GAACTCACAG 
CAACAACAAT CTCGTGATGT GAAACTGGAT GAGAGGTCCA GGAGCGCTAA CAGTAGTGGA 
AGTAATGGCA GCAAATCCAC CAGCTCTTCT AACAAATCAC GATCCACACT TGGGCTTAGT 
CCTCTAATAC ATCCCGACGA ACATGCAGAA CTTATGCCGG GGACGACCGC CAGTGAAGTT 
TTGACTGATT TGAGAACATA CCTTAGAAGC TTTGGGCATC TGGAATCATT GACAGCATCG 
ACTTCAAGGA CACGGCAACG GCAACAATTT TCATCACTCT CCGATCCACA TCCCTATGTC 
GTCAAGTGGG TGGACTATAC GAATAGGTAC GGTATCGGGT ACGTTTTGGA CGACGGAAGC 
GTGGGCTGCG TTTTCAAAGC AGAGCACGGC CGCCCAGCCA GCTGCGTTGT GGTCCGGAAT 
GGAGAACGGC ATATCCGTCG GAAAGCCTAT GCGAAAGAAA ATCCTCGTAG TGCATATGCG 
GATGCCGATC AACTCGTGCC AAGGAATGGT CAACCTGTGG AATTTTATGA AAATGTTGAT 
GTACAAGACA ATTCATACTC TGGCGGTGGA ACGAGAAGGG TGCTTGTTCA GCCGGATGCA 
TTTGAAGTGA GGAATCAAAA TGGCCCCGGT GGGCCCGGCA TAAAAGTTCG GATGAATTCT 
GGAGTTGACT GTGCAAGGTG TGATGCGGAG AAAGTCAAAC GAGTCAAGCT TGTCGACCAG 
TTCGGCAAAT ATATGATTGG AACATTGGGA AGGAGTGTGG ATGAAAACTG CCCTCCCGAG 
GATGAGAGAA ACTATAGATT TTCCGGCAAA ATCGGTCAAT ATGTCAAATT CTATCAGCGA 
CTAGGGAATG TTGGCATATG GGGTTTTGGG GATGGAGCGT TCCAGTTCAA TTTTCCGGAT 
CATACTAAAC TTGTTATTTC ACTGCCACGA CATGGATCGC AAGGCTCGTC GGAGCCACAG 
CCTTGCCAGG CAGATTTTTA TCATCTTTCT CCATCTGCGG CACGCTACCT ATCAACCAGG 
GGCAGAATGC ATCCAGCAGG TTTTGATACC AGAGCCGTTA TATCTGAAAA CCTGAACACC 
TATCTCGCCG CACTCTCAGG TCATCCTATC CACCCATCCG GAATTAGCAG CAGTCGGTTT 
TGCGATATAT TAGAGGCGAA TGCGTTCCGC GACAAATTAG ACTTCGTTCA ACAGGTTCTG 
CGGAGCTGGT TTGAGAGCAG GAGGTTAGGT AACAGAGTAC TGCTACGGAA TACATCATCG 
ACGACCATTA ATACTGGTCC AGATAAAACC ATCCCTGACA ACTTAACATC CAAACGAGGC 
TACCGCGGCG AAATATTTTG GGACGGTGCT CAGGAGAAAT CCTGGTTGAC GCCGTCTGGG 
GGTAAATTTG TGTGGGTTAC TGTTGGCGCC CATGGCGGCG ACGGCGAGTA TTTTGCCGTA 
CAGCTCAAGG GAAATCCTGA AGGCGGAACA GTCGAGTGCG TTGGCGCTGA ACAGATACAA 
GATCTGCAAG ATCAATTGAT GGCGTTAACT GTGCGGTCGG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031577038.1
CDS	290..3814
Translation	MEALSPRSTNILSKPKTDPIMKASAKDAPPQKARSAKNHAPPPPATVREPEDGGEEYITGKFLGKGGFAVCYEGRLARNNRVYAMKVVKSEMNQRKMEEKFRTELQIHSKMRHPNIVGFYRAFAFEQNTYVILELCPNGSVSDMVRKRKYLSLPEVRRFMIQLCGAVKYMHKRNVAHRDLKMGNLFLDRNMDIKVGDFGLAAIILSEKDEKRRRTLCGTPNYIAPEVLDKSKGGHNQKVDIWSLGVIFFAMLTGYPPFQSKTQDEIYKKVKNLTYVWPKESEGANYIPVEAKHLVSSCLNLNEDERPEPDQIVDHAFFNMYNGCIPRQLDPATRLAPPTWLTTQDPRGDRMSHGYSLDHDSKYLGKIAHAKNSNERYLICKNEFYTECGVGRTSTGSIRRPAGKRCSKSAFAECAAEEERGMQPIVPIPEDLVYSYYTGSNGDWSHTTEEDDARPDPDSPIEGEVLPKTRNASYKQQAATVARITSALAAQIRRKEAQTQSHAALLRQQAVPQRQPTMNPSSSQHPQGDIKDTAENGIQTETIPPQRLLNERPIRARRVVSGYSASVRDRNISLTSALPKSNSEPINLTIGKTRSQSRLQMAALTRERNELALEANEVNPLDIRNKDMFASVRGMPARTVRVDPRETERQRPWEQPVNSQQQQSRDVKLDERSRSANSSGSNGSKSTSSSNKSRSTLGLSPLIHPDEHAELMPGTTASEVLTDLRTYLRSFGHLESLTASTSRTRQRQQFSSLSDPHPYVVKWVDYTNRYGIGYVLDDGSVGCVFKAEHGRPASCVVVRNGERHIRRKAYAKENPRSAYADADQLVPRNGQPVEFYENVDVQDNSYSGGGTRRVLVQPDAFEVRNQNGPGGPGIKVRMNSGVDCARCDAEKVKRVKLVDQFGKYMIGTLGRSVDENCPPEDERNYRFSGKIGQYVKFYQRLGNVGIWGFGDGAFQFNFPDHTKLVISLPRHGSQGSSEPQPCQADFYHLSPSAARYLSTRGRMHPAGFDTRAVISENLNTYLAALSGHPIHPSGISSSRFCDILEANAFRDKLDFVQQVLRSWFESRRLGNRVLLRNTSSTTINTGPDKTIPDNLTSKRGYRGEIFWDGAQEKSWLTPSGGKFVWVTVGAHGGDGEYFAVQLKGNPEGGTVECVGAEQIQDLQDQLMALTVRSG

Target ORF information:

RefSeq Version	XM_031720762.1
Organism	Blastomyces gilchristii SLH14081
Definition	Blastomyces gilchristii SLH14081 PLK protein kinase (BDBG_02382), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031720762.1

ORF Insert Sequence:

ATGGAGGCAT TGTCACCTCG CTCAACCAAC ATTCTCTCCA AGCCCAAGAC CGACCCTATA 
ATGAAGGCGA GCGCCAAAGA TGCACCGCCT CAAAAGGCGC GGTCCGCCAA AAACCACGCC 
CCCCCACCTC CTGCTACGGT TCGTGAGCCT GAAGATGGGG GGGAGGAATA CATCACTGGC 
AAATTTTTAG GAAAAGGGGG ATTTGCCGTA TGTTATGAAG GGAGGCTTGC GCGAAATAAC 
AGGGTATATG CGATGAAAGT GGTAAAATCG GAAATGAACC AGAGGAAAAT GGAGGAGAAG 
TTCCGGACCG AGCTCCAGAT ACACTCAAAA ATGCGCCATC CCAACATTGT TGGTTTCTAC 
AGGGCATTTG CCTTCGAACA AAACACTTAT GTCATTTTGG AATTGTGCCC CAATGGCTCG 
GTGTCGGATA TGGTGAGGAA GCGGAAATAC CTGAGCTTGC CCGAAGTCAG GAGATTTATG 
ATTCAGCTTT GCGGGGCGGT TAAATATATG CATAAGAGAA ACGTGGCCCA TCGAGACCTG 
AAAATGGGGA ATCTGTTTCT CGACCGGAAT ATGGACATCA AAGTTGGAGA CTTTGGCCTA 
GCTGCTATTA TCCTGTCGGA GAAGGACGAA AAGCGACGGA GAACACTATG TGGAACTCCA 
AATTATATTG CGCCGGAAGT TTTGGATAAG AGCAAGGGAG GCCATAATCA AAAGGTTGAC 
ATATGGTCGC TTGGCGTAAT TTTTTTTGCC ATGCTCACGG GATACCCCCC ATTTCAATCG 
AAGACGCAGG ACGAAATCTA CAAGAAAGTC AAAAATCTTA CATATGTCTG GCCCAAGGAG 
TCCGAGGGTG CTAATTACAT TCCAGTGGAG GCAAAGCACT TGGTCAGCTC CTGCCTGAAT 
TTGAACGAGG ATGAACGACC GGAGCCCGAC CAGATTGTTG ATCACGCATT TTTCAATATG 
TACAATGGCT GTATACCACG TCAACTGGAC CCAGCCACAC GCCTCGCTCC ACCTACCTGG 
CTGACAACCC AAGACCCTCG AGGAGACCGT ATGAGTCACG GATATAGTCT GGACCATGAC 
TCGAAGTATT TGGGGAAAAT AGCCCATGCG AAAAACTCCA ATGAGCGATA TCTTATTTGC 
AAAAACGAGT TTTACACAGA ATGTGGGGTT GGTAGGACTT CTACTGGATC AATTAGGAGG 
CCAGCAGGGA AACGGTGTTC CAAATCTGCC TTCGCTGAAT GTGCTGCTGA AGAAGAAAGG 
GGGATGCAGC CCATCGTCCC CATACCAGAA GATCTAGTCT ACAGCTATTA CACCGGATCC 
AATGGAGATT GGAGCCATAC AACGGAAGAG GATGATGCTC GGCCCGATCC TGATAGTCCC 
ATTGAGGGTG AAGTGCTTCC CAAAACGCGT AACGCATCGT ATAAACAGCA AGCCGCCACA 
GTTGCTCGCA TAACATCGGC TTTGGCTGCA CAGATTCGCC GCAAAGAAGC TCAAACCCAA 
AGCCATGCAG CTCTCCTACG GCAGCAAGCT GTGCCACAAC GACAGCCAAC AATGAACCCC 
TCGTCTTCCC AACACCCTCA AGGTGATATT AAAGACACCG CAGAAAATGG CATTCAGACC 
GAGACCATTC CTCCCCAGCG GCTGCTGAAT GAGCGACCAA TCCGGGCACG GAGGGTAGTG 
TCTGGTTATT CTGCATCTGT CCGCGACAGG AACATATCGT TGACTTCCGC CTTGCCTAAA 
TCCAATTCGG AACCAATTAA TTTGACCATC GGAAAAACGA GATCACAGTC GAGGCTACAA 
ATGGCAGCGT TGACGCGAGA GAGAAACGAA TTAGCACTGG AAGCCAATGA AGTGAACCCG 
CTAGATATCA GAAATAAAGA CATGTTTGCT TCGGTTCGCG GGATGCCTGC GAGAACAGTT 
AGAGTCGACC CGCGCGAGAC AGAAAGACAG CGACCGTGGG AACAGCCAGT GAACTCACAG 
CAACAACAAT CTCGTGATGT GAAACTGGAT GAGAGGTCCA GGAGCGCTAA CAGTAGTGGA 
AGTAATGGCA GCAAATCCAC CAGCTCTTCT AACAAATCAC GATCCACACT TGGGCTTAGT 
CCTCTAATAC ATCCCGACGA ACATGCAGAA CTTATGCCGG GGACGACCGC CAGTGAAGTT 
TTGACTGATT TGAGAACATA CCTTAGAAGC TTTGGGCATC TGGAATCATT GACAGCATCG 
ACTTCAAGGA CACGGCAACG GCAACAATTT TCATCACTCT CCGATCCACA TCCCTATGTC 
GTCAAGTGGG TGGACTATAC GAATAGGTAC GGTATCGGGT ACGTTTTGGA CGACGGAAGC 
GTGGGCTGCG TTTTCAAAGC AGAGCACGGC CGCCCAGCCA GCTGCGTTGT GGTCCGGAAT 
GGAGAACGGC ATATCCGTCG GAAAGCCTAT GCGAAAGAAA ATCCTCGTAG TGCATATGCG 
GATGCCGATC AACTCGTGCC AAGGAATGGT CAACCTGTGG AATTTTATGA AAATGTTGAT 
GTACAAGACA ATTCATACTC TGGCGGTGGA ACGAGAAGGG TGCTTGTTCA GCCGGATGCA 
TTTGAAGTGA GGAATCAAAA TGGCCCCGGT GGGCCCGGCA TAAAAGTTCG GATGAATTCT 
GGAGTTGACT GTGCAAGGTG TGATGCGGAG AAAGTCAAAC GAGTCAAGCT TGTCGACCAG 
TTCGGCAAAT ATATGATTGG AACATTGGGA AGGAGTGTGG ATGAAAACTG CCCTCCCGAG 
GATGAGAGAA ACTATAGATT TTCCGGCAAA ATCGGTCAAT ATGTCAAATT CTATCAGCGA 
CTAGGGAATG TTGGCATATG GGGTTTTGGG GATGGAGCGT TCCAGTTCAA TTTTCCGGAT 
CATACTAAAC TTGTTATTTC ACTGCCACGA CATGGATCGC AAGGCTCGTC GGAGCCACAG 
CCTTGCCAGG CAGATTTTTA TCATCTTTCT CCATCTGCGG CACGCTACCT ATCAACCAGG 
GGCAGAATGC ATCCAGCAGG TTTTGATACC AGAGCCGTTA TATCTGAAAA CCTGAACACC 
TATCTCGCCG CACTCTCAGG TCATCCTATC CACCCATCCG GAATTAGCAG CAGTCGGTTT 
TGCGATATAT TAGAGGCGAA TGCGTTCCGC GACAAATTAG ACTTCGTTCA ACAGGTTCTG 
CGGAGCTGGT TTGAGAGCAG GAGGTTAGGT AACAGAGTAC TGCTACGGAA TACATCATCG 
ACGACCATTA ATACTGGTCC AGATAAAACC ATCCCTGACA ACTTAACATC CAAACGAGGC 
TACCGCGGCG AAATATTTTG GGACGGTGCT CAGGAGAAAT CCTGGTTGAC GCCGTCTGGG 
GGTAAATTTG TGTGGGTTAC TGTTGGCGCC CATGGCGGCG ACGGCGAGTA TTTTGCCGTA 
CAGCTCAAGG GAAATCCTGA AGGCGGAACA GTCGAGTGCG TTGGCGCTGA ACAGATACAA 
GATCTGCAAG ATCAATTGAT GGCGTTAACT GTGCGGTCGG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BDBG_02382 ( XM_031720763.1 ) cDNA ORF clone, Blastomyces gilchristii SLH14081 -> XP_031577039.1 Blastomyces gilchristii SLH14081 PLK protein kinase, variant (BDBG_02382), mRNA.

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CloneID	OAl116117
Clone ID Related Accession (Same CDS sequence)	XM_031720762.1 , XM_031720763.1
Accession Version	XM_031720763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574697600000
Organism	Blastomyces gilchristii SLH14081
Product	PLK protein kinase, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101680).

ATGGAGGCAT TGTCACCTCG CTCAACCAAC ATTCTCTCCA AGCCCAAGAC CGACCCTATA 
ATGAAGGCGA GCGCCAAAGA TGCACCGCCT CAAAAGGCGC GGTCCGCCAA AAACCACGCC 
CCCCCACCTC CTGCTACGGT TCGTGAGCCT GAAGATGGGG GGGAGGAATA CATCACTGGC 
AAATTTTTAG GAAAAGGGGG ATTTGCCGTA TGTTATGAAG GGAGGCTTGC GCGAAATAAC 
AGGGTATATG CGATGAAAGT GGTAAAATCG GAAATGAACC AGAGGAAAAT GGAGGAGAAG 
TTCCGGACCG AGCTCCAGAT ACACTCAAAA ATGCGCCATC CCAACATTGT TGGTTTCTAC 
AGGGCATTTG CCTTCGAACA AAACACTTAT GTCATTTTGG AATTGTGCCC CAATGGCTCG 
GTGTCGGATA TGGTGAGGAA GCGGAAATAC CTGAGCTTGC CCGAAGTCAG GAGATTTATG 
ATTCAGCTTT GCGGGGCGGT TAAATATATG CATAAGAGAA ACGTGGCCCA TCGAGACCTG 
AAAATGGGGA ATCTGTTTCT CGACCGGAAT ATGGACATCA AAGTTGGAGA CTTTGGCCTA 
GCTGCTATTA TCCTGTCGGA GAAGGACGAA AAGCGACGGA GAACACTATG TGGAACTCCA 
AATTATATTG CGCCGGAAGT TTTGGATAAG AGCAAGGGAG GCCATAATCA AAAGGTTGAC 
ATATGGTCGC TTGGCGTAAT TTTTTTTGCC ATGCTCACGG GATACCCCCC ATTTCAATCG 
AAGACGCAGG ACGAAATCTA CAAGAAAGTC AAAAATCTTA CATATGTCTG GCCCAAGGAG 
TCCGAGGGTG CTAATTACAT TCCAGTGGAG GCAAAGCACT TGGTCAGCTC CTGCCTGAAT 
TTGAACGAGG ATGAACGACC GGAGCCCGAC CAGATTGTTG ATCACGCATT TTTCAATATG 
TACAATGGCT GTATACCACG TCAACTGGAC CCAGCCACAC GCCTCGCTCC ACCTACCTGG 
CTGACAACCC AAGACCCTCG AGGAGACCGT ATGAGTCACG GATATAGTCT GGACCATGAC 
TCGAAGTATT TGGGGAAAAT AGCCCATGCG AAAAACTCCA ATGAGCGATA TCTTATTTGC 
AAAAACGAGT TTTACACAGA ATGTGGGGTT GGTAGGACTT CTACTGGATC AATTAGGAGG 
CCAGCAGGGA AACGGTGTTC CAAATCTGCC TTCGCTGAAT GTGCTGCTGA AGAAGAAAGG 
GGGATGCAGC CCATCGTCCC CATACCAGAA GATCTAGTCT ACAGCTATTA CACCGGATCC 
AATGGAGATT GGAGCCATAC AACGGAAGAG GATGATGCTC GGCCCGATCC TGATAGTCCC 
ATTGAGGGTG AAGTGCTTCC CAAAACGCGT AACGCATCGT ATAAACAGCA AGCCGCCACA 
GTTGCTCGCA TAACATCGGC TTTGGCTGCA CAGATTCGCC GCAAAGAAGC TCAAACCCAA 
AGCCATGCAG CTCTCCTACG GCAGCAAGCT GTGCCACAAC GACAGCCAAC AATGAACCCC 
TCGTCTTCCC AACACCCTCA AGGTGATATT AAAGACACCG CAGAAAATGG CATTCAGACC 
GAGACCATTC CTCCCCAGCG GCTGCTGAAT GAGCGACCAA TCCGGGCACG GAGGGTAGTG 
TCTGGTTATT CTGCATCTGT CCGCGACAGG AACATATCGT TGACTTCCGC CTTGCCTAAA 
TCCAATTCGG AACCAATTAA TTTGACCATC GGAAAAACGA GATCACAGTC GAGGCTACAA 
ATGGCAGCGT TGACGCGAGA GAGAAACGAA TTAGCACTGG AAGCCAATGA AGTGAACCCG 
CTAGATATCA GAAATAAAGA CATGTTTGCT TCGGTTCGCG GGATGCCTGC GAGAACAGTT 
AGAGTCGACC CGCGCGAGAC AGAAAGACAG CGACCGTGGG AACAGCCAGT GAACTCACAG 
CAACAACAAT CTCGTGATGT GAAACTGGAT GAGAGGTCCA GGAGCGCTAA CAGTAGTGGA 
AGTAATGGCA GCAAATCCAC CAGCTCTTCT AACAAATCAC GATCCACACT TGGGCTTAGT 
CCTCTAATAC ATCCCGACGA ACATGCAGAA CTTATGCCGG GGACGACCGC CAGTGAAGTT 
TTGACTGATT TGAGAACATA CCTTAGAAGC TTTGGGCATC TGGAATCATT GACAGCATCG 
ACTTCAAGGA CACGGCAACG GCAACAATTT TCATCACTCT CCGATCCACA TCCCTATGTC 
GTCAAGTGGG TGGACTATAC GAATAGGTAC GGTATCGGGT ACGTTTTGGA CGACGGAAGC 
GTGGGCTGCG TTTTCAAAGC AGAGCACGGC CGCCCAGCCA GCTGCGTTGT GGTCCGGAAT 
GGAGAACGGC ATATCCGTCG GAAAGCCTAT GCGAAAGAAA ATCCTCGTAG TGCATATGCG 
GATGCCGATC AACTCGTGCC AAGGAATGGT CAACCTGTGG AATTTTATGA AAATGTTGAT 
GTACAAGACA ATTCATACTC TGGCGGTGGA ACGAGAAGGG TGCTTGTTCA GCCGGATGCA 
TTTGAAGTGA GGAATCAAAA TGGCCCCGGT GGGCCCGGCA TAAAAGTTCG GATGAATTCT 
GGAGTTGACT GTGCAAGGTG TGATGCGGAG AAAGTCAAAC GAGTCAAGCT TGTCGACCAG 
TTCGGCAAAT ATATGATTGG AACATTGGGA AGGAGTGTGG ATGAAAACTG CCCTCCCGAG 
GATGAGAGAA ACTATAGATT TTCCGGCAAA ATCGGTCAAT ATGTCAAATT CTATCAGCGA 
CTAGGGAATG TTGGCATATG GGGTTTTGGG GATGGAGCGT TCCAGTTCAA TTTTCCGGAT 
CATACTAAAC TTGTTATTTC ACTGCCACGA CATGGATCGC AAGGCTCGTC GGAGCCACAG 
CCTTGCCAGG CAGATTTTTA TCATCTTTCT CCATCTGCGG CACGCTACCT ATCAACCAGG 
GGCAGAATGC ATCCAGCAGG TTTTGATACC AGAGCCGTTA TATCTGAAAA CCTGAACACC 
TATCTCGCCG CACTCTCAGG TCATCCTATC CACCCATCCG GAATTAGCAG CAGTCGGTTT 
TGCGATATAT TAGAGGCGAA TGCGTTCCGC GACAAATTAG ACTTCGTTCA ACAGGTTCTG 
CGGAGCTGGT TTGAGAGCAG GAGGTTAGGT AACAGAGTAC TGCTACGGAA TACATCATCG 
ACGACCATTA ATACTGGTCC AGATAAAACC ATCCCTGACA ACTTAACATC CAAACGAGGC 
TACCGCGGCG AAATATTTTG GGACGGTGCT CAGGAGAAAT CCTGGTTGAC GCCGTCTGGG 
GGTAAATTTG TGTGGGTTAC TGTTGGCGCC CATGGCGGCG ACGGCGAGTA TTTTGCCGTA 
CAGCTCAAGG GAAATCCTGA AGGCGGAACA GTCGAGTGCG TTGGCGCTGA ACAGATACAA 
GATCTGCAAG ATCAATTGAT GGCGTTAACT GTGCGGTCGG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031577039.1
CDS	324..3848
Translation	MEALSPRSTNILSKPKTDPIMKASAKDAPPQKARSAKNHAPPPPATVREPEDGGEEYITGKFLGKGGFAVCYEGRLARNNRVYAMKVVKSEMNQRKMEEKFRTELQIHSKMRHPNIVGFYRAFAFEQNTYVILELCPNGSVSDMVRKRKYLSLPEVRRFMIQLCGAVKYMHKRNVAHRDLKMGNLFLDRNMDIKVGDFGLAAIILSEKDEKRRRTLCGTPNYIAPEVLDKSKGGHNQKVDIWSLGVIFFAMLTGYPPFQSKTQDEIYKKVKNLTYVWPKESEGANYIPVEAKHLVSSCLNLNEDERPEPDQIVDHAFFNMYNGCIPRQLDPATRLAPPTWLTTQDPRGDRMSHGYSLDHDSKYLGKIAHAKNSNERYLICKNEFYTECGVGRTSTGSIRRPAGKRCSKSAFAECAAEEERGMQPIVPIPEDLVYSYYTGSNGDWSHTTEEDDARPDPDSPIEGEVLPKTRNASYKQQAATVARITSALAAQIRRKEAQTQSHAALLRQQAVPQRQPTMNPSSSQHPQGDIKDTAENGIQTETIPPQRLLNERPIRARRVVSGYSASVRDRNISLTSALPKSNSEPINLTIGKTRSQSRLQMAALTRERNELALEANEVNPLDIRNKDMFASVRGMPARTVRVDPRETERQRPWEQPVNSQQQQSRDVKLDERSRSANSSGSNGSKSTSSSNKSRSTLGLSPLIHPDEHAELMPGTTASEVLTDLRTYLRSFGHLESLTASTSRTRQRQQFSSLSDPHPYVVKWVDYTNRYGIGYVLDDGSVGCVFKAEHGRPASCVVVRNGERHIRRKAYAKENPRSAYADADQLVPRNGQPVEFYENVDVQDNSYSGGGTRRVLVQPDAFEVRNQNGPGGPGIKVRMNSGVDCARCDAEKVKRVKLVDQFGKYMIGTLGRSVDENCPPEDERNYRFSGKIGQYVKFYQRLGNVGIWGFGDGAFQFNFPDHTKLVISLPRHGSQGSSEPQPCQADFYHLSPSAARYLSTRGRMHPAGFDTRAVISENLNTYLAALSGHPIHPSGISSSRFCDILEANAFRDKLDFVQQVLRSWFESRRLGNRVLLRNTSSTTINTGPDKTIPDNLTSKRGYRGEIFWDGAQEKSWLTPSGGKFVWVTVGAHGGDGEYFAVQLKGNPEGGTVECVGAEQIQDLQDQLMALTVRSG

Target ORF information:

RefSeq Version	XM_031720763.1
Organism	Blastomyces gilchristii SLH14081
Definition	Blastomyces gilchristii SLH14081 PLK protein kinase, variant (BDBG_02382), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031720763.1

ORF Insert Sequence:

ATGGAGGCAT TGTCACCTCG CTCAACCAAC ATTCTCTCCA AGCCCAAGAC CGACCCTATA 
ATGAAGGCGA GCGCCAAAGA TGCACCGCCT CAAAAGGCGC GGTCCGCCAA AAACCACGCC 
CCCCCACCTC CTGCTACGGT TCGTGAGCCT GAAGATGGGG GGGAGGAATA CATCACTGGC 
AAATTTTTAG GAAAAGGGGG ATTTGCCGTA TGTTATGAAG GGAGGCTTGC GCGAAATAAC 
AGGGTATATG CGATGAAAGT GGTAAAATCG GAAATGAACC AGAGGAAAAT GGAGGAGAAG 
TTCCGGACCG AGCTCCAGAT ACACTCAAAA ATGCGCCATC CCAACATTGT TGGTTTCTAC 
AGGGCATTTG CCTTCGAACA AAACACTTAT GTCATTTTGG AATTGTGCCC CAATGGCTCG 
GTGTCGGATA TGGTGAGGAA GCGGAAATAC CTGAGCTTGC CCGAAGTCAG GAGATTTATG 
ATTCAGCTTT GCGGGGCGGT TAAATATATG CATAAGAGAA ACGTGGCCCA TCGAGACCTG 
AAAATGGGGA ATCTGTTTCT CGACCGGAAT ATGGACATCA AAGTTGGAGA CTTTGGCCTA 
GCTGCTATTA TCCTGTCGGA GAAGGACGAA AAGCGACGGA GAACACTATG TGGAACTCCA 
AATTATATTG CGCCGGAAGT TTTGGATAAG AGCAAGGGAG GCCATAATCA AAAGGTTGAC 
ATATGGTCGC TTGGCGTAAT TTTTTTTGCC ATGCTCACGG GATACCCCCC ATTTCAATCG 
AAGACGCAGG ACGAAATCTA CAAGAAAGTC AAAAATCTTA CATATGTCTG GCCCAAGGAG 
TCCGAGGGTG CTAATTACAT TCCAGTGGAG GCAAAGCACT TGGTCAGCTC CTGCCTGAAT 
TTGAACGAGG ATGAACGACC GGAGCCCGAC CAGATTGTTG ATCACGCATT TTTCAATATG 
TACAATGGCT GTATACCACG TCAACTGGAC CCAGCCACAC GCCTCGCTCC ACCTACCTGG 
CTGACAACCC AAGACCCTCG AGGAGACCGT ATGAGTCACG GATATAGTCT GGACCATGAC 
TCGAAGTATT TGGGGAAAAT AGCCCATGCG AAAAACTCCA ATGAGCGATA TCTTATTTGC 
AAAAACGAGT TTTACACAGA ATGTGGGGTT GGTAGGACTT CTACTGGATC AATTAGGAGG 
CCAGCAGGGA AACGGTGTTC CAAATCTGCC TTCGCTGAAT GTGCTGCTGA AGAAGAAAGG 
GGGATGCAGC CCATCGTCCC CATACCAGAA GATCTAGTCT ACAGCTATTA CACCGGATCC 
AATGGAGATT GGAGCCATAC AACGGAAGAG GATGATGCTC GGCCCGATCC TGATAGTCCC 
ATTGAGGGTG AAGTGCTTCC CAAAACGCGT AACGCATCGT ATAAACAGCA AGCCGCCACA 
GTTGCTCGCA TAACATCGGC TTTGGCTGCA CAGATTCGCC GCAAAGAAGC TCAAACCCAA 
AGCCATGCAG CTCTCCTACG GCAGCAAGCT GTGCCACAAC GACAGCCAAC AATGAACCCC 
TCGTCTTCCC AACACCCTCA AGGTGATATT AAAGACACCG CAGAAAATGG CATTCAGACC 
GAGACCATTC CTCCCCAGCG GCTGCTGAAT GAGCGACCAA TCCGGGCACG GAGGGTAGTG 
TCTGGTTATT CTGCATCTGT CCGCGACAGG AACATATCGT TGACTTCCGC CTTGCCTAAA 
TCCAATTCGG AACCAATTAA TTTGACCATC GGAAAAACGA GATCACAGTC GAGGCTACAA 
ATGGCAGCGT TGACGCGAGA GAGAAACGAA TTAGCACTGG AAGCCAATGA AGTGAACCCG 
CTAGATATCA GAAATAAAGA CATGTTTGCT TCGGTTCGCG GGATGCCTGC GAGAACAGTT 
AGAGTCGACC CGCGCGAGAC AGAAAGACAG CGACCGTGGG AACAGCCAGT GAACTCACAG 
CAACAACAAT CTCGTGATGT GAAACTGGAT GAGAGGTCCA GGAGCGCTAA CAGTAGTGGA 
AGTAATGGCA GCAAATCCAC CAGCTCTTCT AACAAATCAC GATCCACACT TGGGCTTAGT 
CCTCTAATAC ATCCCGACGA ACATGCAGAA CTTATGCCGG GGACGACCGC CAGTGAAGTT 
TTGACTGATT TGAGAACATA CCTTAGAAGC TTTGGGCATC TGGAATCATT GACAGCATCG 
ACTTCAAGGA CACGGCAACG GCAACAATTT TCATCACTCT CCGATCCACA TCCCTATGTC 
GTCAAGTGGG TGGACTATAC GAATAGGTAC GGTATCGGGT ACGTTTTGGA CGACGGAAGC 
GTGGGCTGCG TTTTCAAAGC AGAGCACGGC CGCCCAGCCA GCTGCGTTGT GGTCCGGAAT 
GGAGAACGGC ATATCCGTCG GAAAGCCTAT GCGAAAGAAA ATCCTCGTAG TGCATATGCG 
GATGCCGATC AACTCGTGCC AAGGAATGGT CAACCTGTGG AATTTTATGA AAATGTTGAT 
GTACAAGACA ATTCATACTC TGGCGGTGGA ACGAGAAGGG TGCTTGTTCA GCCGGATGCA 
TTTGAAGTGA GGAATCAAAA TGGCCCCGGT GGGCCCGGCA TAAAAGTTCG GATGAATTCT 
GGAGTTGACT GTGCAAGGTG TGATGCGGAG AAAGTCAAAC GAGTCAAGCT TGTCGACCAG 
TTCGGCAAAT ATATGATTGG AACATTGGGA AGGAGTGTGG ATGAAAACTG CCCTCCCGAG 
GATGAGAGAA ACTATAGATT TTCCGGCAAA ATCGGTCAAT ATGTCAAATT CTATCAGCGA 
CTAGGGAATG TTGGCATATG GGGTTTTGGG GATGGAGCGT TCCAGTTCAA TTTTCCGGAT 
CATACTAAAC TTGTTATTTC ACTGCCACGA CATGGATCGC AAGGCTCGTC GGAGCCACAG 
CCTTGCCAGG CAGATTTTTA TCATCTTTCT CCATCTGCGG CACGCTACCT ATCAACCAGG 
GGCAGAATGC ATCCAGCAGG TTTTGATACC AGAGCCGTTA TATCTGAAAA CCTGAACACC 
TATCTCGCCG CACTCTCAGG TCATCCTATC CACCCATCCG GAATTAGCAG CAGTCGGTTT 
TGCGATATAT TAGAGGCGAA TGCGTTCCGC GACAAATTAG ACTTCGTTCA ACAGGTTCTG 
CGGAGCTGGT TTGAGAGCAG GAGGTTAGGT AACAGAGTAC TGCTACGGAA TACATCATCG 
ACGACCATTA ATACTGGTCC AGATAAAACC ATCCCTGACA ACTTAACATC CAAACGAGGC 
TACCGCGGCG AAATATTTTG GGACGGTGCT CAGGAGAAAT CCTGGTTGAC GCCGTCTGGG 
GGTAAATTTG TGTGGGTTAC TGTTGGCGCC CATGGCGGCG ACGGCGAGTA TTTTGCCGTA 
CAGCTCAAGG GAAATCCTGA AGGCGGAACA GTCGAGTGCG TTGGCGCTGA ACAGATACAA 
GATCTGCAAG ATCAATTGAT GGCGTTAACT GTGCGGTCGG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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BDBG_02382 cDNA ORF clone, Blastomyces gilchristii SLH14081

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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