BDBG_01634 cDNA ORF clone, Blastomyces gilchristii SLH14081

Gene
Clones
gRNAs

Summary

Gene Symbol	BDBG_01634
Entrez Gene ID	8507531
Full Name	bifunctional pyrimidine biosynthesis protein pyrABCN
Gene Type	protein-coding
Organism	Blastomyces gilchristii SLH14081

mRNA and Protein(s)

mRNA	Protein	Name
XM_031720420.1	XP_031576597.1	protein pyrABCN
XM_002628680.1	XP_002628726.1	bifunctional pyrimidine biosynthesis protein pyrABCN

Pathways from BioSystems
Gene Ontology

The following BDBG_01634 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BDBG_01634 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl06335	XM_002628680.1 Latest version!	Ajellomyces dermatitidis SLH14081 bifunctional pyrimidine biosynthesis protein pyrABCN, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAl116695	XM_031720420.1 Latest version!	Blastomyces gilchristii SLH14081 protein pyrABCN (BDBG_01634), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BDBG_01634 ( XM_002628680.1 ) cDNA ORF clone, Blastomyces gilchristii SLH14081 -> XP_002628726.1 Ajellomyces dermatitidis SLH14081 bifunctional pyrimidine biosynthesis protein pyrABCN, mRNA.

Price & Availability↑

CloneID	OAl06335
Clone ID Related Accession (Same CDS sequence)	XM_002628680.1
Accession Version	XM_002628680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254931200000
Organism	Blastomyces gilchristii SLH14081
Product	bifunctional pyrimidine biosynthesis protein pyrABCN
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101681). COMPLETENESS: incomplete on the 5' end.

ATGCCGCTTC TCAATGCCGC TGGGGATGCC AAAAGCTCTC CTGGCCAGTC TCATTCCGTC 
AGGAGCAGAC GACTAACCGA TACTACTGTT ACTCCGTCCA GCCCTGCTTC TGGTGGCGTC 
GTCGCATACA CCACCCAGAA TAAAGACCTG CCTTCCCTCG CTCCCCAAAC GGCATATTCC 
CTCAATGGCG GTGCTATCCC CCCCGCCTCT GCCCCAATGG CTGGCGAAGG GGGAGATGGG 
AGCAAGTGGT ATGCCCTAGA GCTGGAGGAT GGCACCGTCT ACCAGGGTTA CAGTTTTGGT 
GCCGAAGCGA GCATTGCTGG CGAGTTGGTT TTTCAAACTG GCATGGTGGG CTACCCAGAA 
TCAATCACAG ATCCTTCCTA CCGTGGCCAG ATCTTGATCA TCACCTTCCC GCTGGTAGGC 
AACTATGGCG TCCCTTCGCG AGAAACGGTT GATGAGCTCC TCGGGCAAGA TATGCCCAAA 
TATTTCGAGT CCAGTGAGAT CCATGTTGCT GGTCTTGTAG TGGCCAATTA CTGCGGAGAG 
GACTACTCGC ACTTCTTGGC CGAGTCGTCT CTCGGGAAGT GGTTGAAGGA ACAAGGAGTT 
CCTGCCATGT ATGGTGTTGA TACCCGCGCC CTCACAAAGA GAATTCGAGA GAAGGGAAGC 
ATGCTTGGCC GCATGCTTTT GCAGAAACCC GGTGTGAGTG TTGAAACCTT GAATAGCGAC 
GCGCCGAAAA ACGGCTCGAC TACTGCTGTT GTTGATGACG AAGAGAAATC CGATCTTTGG 
AGAGCATCCT TTGAACAAAT TGAATGGATG GACCCTGATA AGAGGAACCT GGTCAACGAT 
GTCTCTATTA GGAAACCCCA GCTGTTCTCC CCACCAAAGG ATTCGGTCCT TTCTCACCCC 
TGTGGTCGCC CGATCAGAGT TGTGTGCGTT GACGTTGGTA TGAAATATAA CCAACTACGG 
TGCCTTCTCG CTCGCGGCGT CGAGCTCCTT GTTGTACCAT GGGATTATGA TTTCCCCAGC 
TTGGCAGGAA AGGAATATGA CGGCCTTTTC CTTTCCAACG GCCCGGGCAA CCCCGCCACC 
CTGTCCAAAA CAGTGGAGAA TATTAGAACC GCCATCAAGG ACTCGCGCAC TCCAATCTTT 
GGTATTTGTC TTGGACACCA GTTACTTGCC CGGGCTGCGG GTGCGTCTAC CATTAAGATG 
AAGTTCGGTA ACCGAGGACA AAATATCCCC TGTACCAGCC TTATCTCCGG TCGCTGTTAC 
ATTACTTCGC AAAACCACGG ATATGCAGTT GATTCTATGA CATTGCCAGA GGGATGGGAG 
GAGCTCTTTG TCAACGCTAA TGATGGAAGC AACGAAGGTA TCAGACACGT TAGCCGCCCA 
TTTTTCAGTG TCCAATTCCA CCCAGAGAGC GCCCCAGGAC CCCAGGACAC AGAGTTCCTA 
TTCGATGTCT TCATCGATGC TATTAAAACG AACGTGGCAT TCCCTAGTGC CTTAGAGAAG 
CCCGTTAGCT TCCCTGGCGC CACCGCTAAA GAGAACGAGC TTGCAAACCC GAGAGTGAGC 
GCTAAGAAAG CCCTTATCCT AGGTAGCGGT GGTCTCAGTA TCGGACAAGC TGGAGAGTTT 
GACTATTCTG GAAGTCAAGC CATAAAGGCT TTGAAAGAAG AAGGAATTTA CACCGTCCTT 
ATTAACCCCA ATATCGCGAC GATTCAGACT TCCAAGGGCC TTGCAGATAA GGTCTATTTC 
TTACCAGTCA ACGCAGACTT TGTCCGCAAG GTTATCAAAC ACGAACGGCC AGATGCCATC 
TATGTAACAT TTGGCGGCCA AACTGCGCTC CAGGTTGGTA TTCAGCTCAA AGATGAGTTC 
GAGGAACTTG GCGTTCGAGT TCTTGGTACT CCGATAGACA CCATCATTAC GACAGAAGAC 
CGCGAGTTAT TCGCGAGAAG CATGGAAGCC CTTGGTGAGA AGTGCGCCAA ATCCGCCTCT 
GCTTCCACGC TCGAGGAAGC CATGGAAGTT GTCAAGGATA TTGGATTTCC CGTCATTGTC 
CGTGCTGCGT ACGCTCTTGG TGGCCTTGGA AGCGGTTTCG CCGAGGATCC TGAGCAGCTG 
CGGGAGCTGT GCACAAAAGC CTTCGCTGCA AGCCCACAGG TTCTTATTGA GAGGAGTATG 
AAAGGGTGGA AGGAGATTGA ATATGAAGTG GTCCGCGATG CTCGTGATAA CTGCATCACT 
GTGTGTAACA TGGAGAATTT TGATCCGCTT GGTATCCACA CTGGCGATTC TATTGTCGTC 
GCCCCGTCGC AAACCCTTTC TGATGAGGAC TACAATATGC TTCGAACGAC TGCTATCAAT 
GTCATCCGCC ATCTGGGTGT GGTGGGTGAA TGTAACATTC AATATGCATT GAACCCATTC 
TCAAAAGAGT ATTGCATTAT TGAGGTCAAT GCTCGCCTGT CAAGATCATC TGCACTCGCA 
TCTAAAGCAA CAGGCTATCC CCTCGCTTTC GTCGCGGCTA AACTTGGCCT CGGAATCCCG 
CTGAATGAGA TTTCAAACTC AGTCACCAAA GTCACATGTG CCTGCTTTGA ACCTTCTCTT 
GATTATGTCG TCGTTAAGAT TCCCCGCTGG GATCTCAAGA AGTTTACCCG TGTCTCCACG 
CAGCTCGGGT CTTCAATGAA GAGTGTTGGG GAGGTTATGA GCATTGGACG GACTTTTGAG 
GAGGCCATTC AGAAAGCCAT ACGTTCTGTC GACTTTCACA ATCTTGGGTT CAACGAGACC 
AACGCACTCA TGTCGATTAA GGCTGAGCTT CAAACACCTT CCGATCAGCG ACTGTTCGCT 
ATCGCCAACG CCATGCATTC GGGCTACACT GTCGACGATA TCTGGGAATT GACCAAAATC 
GACAAGTGGT TCCTCCGAAA ATTGAAAGGC CTCAGCGATT TTGGAAAACT AATGTCTGGT 
TACAACGCCA CTACAGCACC CATTTCTCTC ATTCGACAGG CGAAGCAGCT CGGTTTCTCG 
GACCGGCAAC TGGCGAAGTT TTTGAGCTCC AATGAGTTGG CTATCAGGCG ACTGAGAGTG 
GAAGCAGGCA TTGTGCCCGT GGTCAAACAA ATTGATACCG TCGCTGCTGA ATTCCCGGCA 
TTCACGAACT ACTTGTATCT GACATACAAC GGCTCCGAAA ACGATGTGGA CTTTAACGAC 
CAAGGTGTTA TGGTTCTTGG CTCTGGTGTC TACAGAATTG GATCCTCTGT CGAGTTTGAT 
TGGTGTTCTG TGCGAGCCAT TCGAACTCTT CGAGAGCAGG GTTTTAAGAC GGTGATGGTG 
AATGTTCACA ATCTCCTAGA TTTCACCATC TCCTATATTT CTCAAAAGCT CTGCAAGATT 
TCCCAAAAGC TCCCCAAGGT CCTCTGCTGC TCTGCCTCCA CCAGTTTGTT TCCTGGTTGC 
TTGCTCCTGA GACGATTCGA TTTCTATGGC TTCTCCTGTG TTCTCTGCTC CGACGGTATT 
CAAGCTCATC AGCTTGTTGC TTGGAAATGC CTTTGTGATC CCCGTCCACG CTTCAAACAT 
CCTCTATTTG CAACTTCTAT AGCCAGGAAG AGCTGGAGGC AATTGCTGAG GGGATCTCTC 
TCTCTCTCCT TTGTCTCCGG CATTTTTGAC CTGCTGAAAG CCCCAAACCC CGCCCTTCGA 
GGCCCGCAAC GCCAACTGGG ACGCCCACAT ACTTTGGGCC GCCTGATGTT TCTAATTCTC 
GAAGCCACCC TTGCACATAT TCTGGGCGAT GAGTGCGAAA ATGGAAACTA CAGGATGGGC 
CATATCTGTT TTTGGGATCG CGAAAAACTC GAATATGACG GCGTCTGCAG TCATTGTGCT 
TTGGACGAAG GCATTCGCGG CGAGTTCGAC CTGTCTCCTG AGGAATATGA AATATATGCA 
AAAGAACGCG AAATCAAGCA TCGTGAAATC CACCGGGACG GCAATTCAAG CTGGCATTAT 
AAGCAGATGG CAGGGAACTA TCCGGATTAT ATTGAGGCAG CCCATGAGCG ATCCAACCCC 
GAGACTCTCA GCACAGATTA CGACGAAGCA GACCGACTGT ATTTTGAAAA TATCGATTTG 
GAGTCCGTCC TTGATGTCTA CCAACTTGAG TCTGCAAGCG GGGTTATTGT ATCTATGGGA 
GGACAGGCAC CGAGCAACAT CGCTCTTCCC TTACACAGAC TCCATGTTAA TGTCCTTGGT 
ACATCTCCGG AGATGATTGA TACAGCTGAG AATCGGTACA AATTTTCGCG ACTCCTTGAC 
CATATTGAGG TCGATCAGCC AGCTTGGAAG GAATTGACCA GTATCGAGGA GGCCACCGAA 
TTCTGTGCGA AGGTTGGATA CCCAGTACTG GTGCGGCCTT CGTACGTGCT CTCTGGTGCT 
GCCATGAACA CGGTTTATTC TTTGGATGAT CTTGCAAGCT ACCTCAACCA GGCTGTTGAG 
GTCTCCCGTG ATCACCCTGT GGTGATTACC AAATACATAG AGAACGCCAA GGAAATTGAA 
ATGGACGCAG TTGCCAAGAA TGGTGTTATG GTCGGCCATT TTATTTCAGA ACATGTTGAG 
AATGCGGGTG TCCATTCTGG CGATGCTACT TTGATTCTTC CGCCCCAGGA CTTGGACCCG 
GAAACCGTCC GGCGAATCGA GGAAGCGACC CGCAAGATCG GCAATGCCCT GAACGTCACT 
GGACCTTTCA ATATCCAGTT CATCGCCAAA GATAACGACA TTAAAGTTAT CGAGTGCAAT 
GTCAGAGCAT CTCGATCCTT CCCGTTCGTT TCCAAAGTCA TGGGAGTTGA CTTGATCGAA 
ATGGCAACGA TGGCAATGAT TGGCAAGCCG TTCACTGAGT ACCCGCCGGT AACCATTCCC 
AAGAACTACG TCGGTGTCAA GGTTCCGCAA TTCTCATTCT CCCGCCTCTC TGGTGCTGAC 
CCCGTGCTCG GAGTGGAGAT GGCTTCTACC GGTGAAGTTG CTTGCTTCGG TCGTGACAGG 
TATGAGGCAT ATCTCAAAGC TCTGATTTCG ACTGGCTTCA AGCTTCCGAA AAAGAACGTC 
CTGTTCTCCA TCGGTTCCTT CAAGGAGAAG GTAGAGATGT TGCCGTCTAT CAGAAAGCTG 
CACGCGCTTG GCTACAACCT TTTTGCCACG GCGGGCACTG CGGACTATCT CAAAGAGCAC 
GGACTTCCGG TCAAATACCT CGAAGTTCTT GCGGGAGAGG AGGAGGACCT TAAGTCTGAA 
TATTCTCTTA CGCAGCATTT GGCCAACAAT CTGATTGATC TTTACATCAA TCTTCCCTCC 
AGCAATAGGT TCCGGCGGCC TGCAAACTAC ATGAGCAAGG GCTATCGAAC ACGGCGAATG 
GCAGTCGACT ACCAGACCCC GCTAGTCACC AACGTTAAGA ACGCGAAAAT CCTGATCGAA 
GCGATTGCAC GCCATTACAC TATGAACGTG ATGAGCATTG ATTGCCAAAC GAGCTATCGC 
GCTGTTACTA TCCCCGGCCT AATCAACGTC GCAGCGTTCG TGCCAGGACT CGCTAACCCA 
GATTCTCCAG ATTTCCAGGT GGTGACTAAG GCTTCCATTG CGAACGGTTT TAGCATGGTC 
CGCATCATGC CCGTGGGCGT GGATAGCTTC GTTACTGACT CTCAGGCGTT GAGAACGGCC 
CAACATAATT CGTCAAAGGG ATCGTACTGC GATTTCAACC TCTCTGTCAC GGCAACATCT 
AATAATTCAG ATAAGCTCGG CCTAGTCACC AGTGAAGTCG GTTCGTTATT TATCCCATTC 
AACCACCTAT CAGGCAACAT CAACAAGGTG GCAACGGTTA CTTCGCACTT TGCATCATGG 
CCGACCAATA AACCTATAAT CACCGATGCC AAAGCTACGG ACCTGGCTTC CATTTTGCTG 
CTTGCCAGTC TTCACAATCG CAATATCCAC GTCATGAGCG TTACTAGCAA AGAGGACATG 
GGCCTGATTG CGCTTAGCAA GGAAAAGGGT CTGAAAGTGA CTTGCGATGT TGCAATCTAC 
TCCCTTTTCC TGTCTACCGA TGATTACCCT GGTTGCGCAT GCCTTCCTTC GAAGAAAGAC 
CAAGAAGTGC TCTGGGAGCA CTTGGATACG ATTGATATAT TTTCTATCGG TAGTCTTCCA 
TACCAGGTTG CGGGTAGCGA TGCTTCTCCC GCTGTTGGGA TCCAGGATGC GCTTCCTCTG 
CTCTTCACAG CAGTTTCTGA CAGGCGACTA ACTATCGAGG ATGTTACCGC CAGACTCCAC 
GATAACCCGA AGAGGATCTT TGAACTTCAT GACCAGCCAG ATGCCTCCGT GGAGATCGAG 
TTTGACAGGC CATATGTATT CCAGAGCCAT GATGTCTGGT CACCATTTGC CGGTAAGACT 
CTAAAGGCAT CCGTCCGACG AGTTACATTC CAGGGGAAGA CCTCTTGCTT GGACGGTGAA 
ATGATGCTGG ATGCACCGAA GGGGGCCGAC ATGTCCTCTC ATCGGTTAGC GCCACCGGCT 
CCAGCATCGC CGGCAGTCAA AGACATTCTT CCCACCTCTC CTCGATCGGA AAGTGCCTTT 
GGGAGACGTT TGTCTTTCTC GGCCACACCG CTTCAGCGCC CGTCGAGATT CAAACCTGCT 
GCTCCTGATG GATATGCTCC ATCTGTCTCA GGAGACGACT TTGTGCCTGC CGCTCCGATT 
TACCCATATC CGTCCCGGAT TTCACCCTCA TTGCAACAGC TGCTTTCACA ATCGTCTTTC 
AAGCAGAAGC ATATCCTATC TGTCAGCCAG TTCACCCGCC CAGATCTTCA CCTTCTATTC 
ACGGTTGCGC AAGAGATGAG GCTGGGTGTC CAACGCCATG GTGTCCTTGA CATCCTGAAG 
GGCCGCGTCC TCACAACCCT CTTCTTCGAG CCCTCCACTC GTACGTCTGC TTCGTTCAAT 
GCCGCTATGC AACGACTGGG TGGCCGCACC ATCCCCGTCG CGACTGAGCA TTCGTCCACT 
CAAAAGGGCG AAACCCTTCA GGACACTGTA CGCACCCTGG GCTGCTACAG CGACGCGATC 
GTCCTCCGTC ATCCGGATGA CAGCAGCGCT GCCACCGCCG CCAAATTCTC TCCAGTTCCC 
ATCATCAACG GCGGAAACGG AGCCATGGAA CACCCCACCC AAGCCTTCCT CGACCTCTTC 
ACCATCCGCG AGGAACTCGG CACCGTCAAC GACATCACAA TTACCTTTAC CGGCGATTTA 
AAATACGGCC GCACCGTGCA CTCCCTCGTC AAACTCCTCC AATTCTACGA AGTTCGCGTC 
CAGCTCGTCT CGCCCAAGGC GCTCGCTGTC CCCGAGGAGG TCCGTCAACA GCTCGTCTCG 
TCCGGCCAGC TGATCCTGGA GTCGACGGAG CTTACTCCGC AGATCATCGC ACGGTCGGAT 
GTGCTGTACT GCACCCGGGT GCAGAAGGAG CGGTTCGAGG ACTTGGGCGA GTATGAGCGG 
TTGAAGAATG CATTCGTCAT CGATAACACA GTCCTTAAGC ATGCGAAGAG CCAGATGATC 
GTTATGCATC CTCTGCCGCG CAACCAGGAG GTCTGCGAGG AGGTCGATTT TGACCAGCGC 
GCGGCTTATT TCCGACAGAT GAAATATGGG CTCTACTGTC GCATGGCATT ACTGGCATTG 
GTCATGGCAT CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002628726.1
CDS	1..7635
Translation	MPLLNAAGDAKSSPGQSHSVRSRRLTDTTVTPSSPASGGVVAYTTQNKDLPSLAPQTAYSLNGGAIPPASAPMAGEGGDGSKWYALELEDGTVYQGYSFGAEASIAGELVFQTGMVGYPESITDPSYRGQILIITFPLVGNYGVPSRETVDELLGQDMPKYFESSEIHVAGLVVANYCGEDYSHFLAESSLGKWLKEQGVPAMYGVDTRALTKRIREKGSMLGRMLLQKPGVSVETLNSDAPKNGSTTAVVDDEEKSDLWRASFEQIEWMDPDKRNLVNDVSIRKPQLFSPPKDSVLSHPCGRPIRVVCVDVGMKYNQLRCLLARGVELLVVPWDYDFPSLAGKEYDGLFLSNGPGNPATLSKTVENIRTAIKDSRTPIFGICLGHQLLARAAGASTIKMKFGNRGQNIPCTSLISGRCYITSQNHGYAVDSMTLPEGWEELFVNANDGSNEGIRHVSRPFFSVQFHPESAPGPQDTEFLFDVFIDAIKTNVAFPSALEKPVSFPGATAKENELANPRVSAKKALILGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTVLINPNIATIQTSKGLADKVYFLPVNADFVRKVIKHERPDAIYVTFGGQTALQVGIQLKDEFEELGVRVLGTPIDTIITTEDRELFARSMEALGEKCAKSASASTLEEAMEVVKDIGFPVIVRAAYALGGLGSGFAEDPEQLRELCTKAFAASPQVLIERSMKGWKEIEYEVVRDARDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAINVIRHLGVVGECNIQYALNPFSKEYCIIEVNARLSRSSALASKATGYPLAFVAAKLGLGIPLNEISNSVTKVTCACFEPSLDYVVVKIPRWDLKKFTRVSTQLGSSMKSVGEVMSIGRTFEEAIQKAIRSVDFHNLGFNETNALMSIKAELQTPSDQRLFAIANAMHSGYTVDDIWELTKIDKWFLRKLKGLSDFGKLMSGYNATTAPISLIRQAKQLGFSDRQLAKFLSSNELAIRRLRVEAGIVPVVKQIDTVAAEFPAFTNYLYLTYNGSENDVDFNDQGVMVLGSGVYRIGSSVEFDWCSVRAIRTLREQGFKTVMVNVHNLLDFTISYISQKLCKISQKLPKVLCCSASTSLFPGCLLLRRFDFYGFSCVLCSDGIQAHQLVAWKCLCDPRPRFKHPLFATSIARKSWRQLLRGSLSLSFVSGIFDLLKAPNPALRGPQRQLGRPHTLGRLMFLILEATLAHILGDECENGNYRMGHICFWDREKLEYDGVCSHCALDEGIRGEFDLSPEEYEIYAKEREIKHREIHRDGNSSWHYKQMAGNYPDYIEAAHERSNPETLSTDYDEADRLYFENIDLESVLDVYQLESASGVIVSMGGQAPSNIALPLHRLHVNVLGTSPEMIDTAENRYKFSRLLDHIEVDQPAWKELTSIEEATEFCAKVGYPVLVRPSYVLSGAAMNTVYSLDDLASYLNQAVEVSRDHPVVITKYIENAKEIEMDAVAKNGVMVGHFISEHVENAGVHSGDATLILPPQDLDPETVRRIEEATRKIGNALNVTGPFNIQFIAKDNDIKVIECNVRASRSFPFVSKVMGVDLIEMATMAMIGKPFTEYPPVTIPKNYVGVKVPQFSFSRLSGADPVLGVEMASTGEVACFGRDRYEAYLKALISTGFKLPKKNVLFSIGSFKEKVEMLPSIRKLHALGYNLFATAGTADYLKEHGLPVKYLEVLAGEEEDLKSEYSLTQHLANNLIDLYINLPSSNRFRRPANYMSKGYRTRRMAVDYQTPLVTNVKNAKILIEAIARHYTMNVMSIDCQTSYRAVTIPGLINVAAFVPGLANPDSPDFQVVTKASIANGFSMVRIMPVGVDSFVTDSQALRTAQHNSSKGSYCDFNLSVTATSNNSDKLGLVTSEVGSLFIPFNHLSGNINKVATVTSHFASWPTNKPIITDAKATDLASILLLASLHNRNIHVMSVTSKEDMGLIALSKEKGLKVTCDVAIYSLFLSTDDYPGCACLPSKKDQEVLWEHLDTIDIFSIGSLPYQVAGSDASPAVGIQDALPLLFTAVSDRRLTIEDVTARLHDNPKRIFELHDQPDASVEIEFDRPYVFQSHDVWSPFAGKTLKASVRRVTFQGKTSCLDGEMMLDAPKGADMSSHRLAPPAPASPAVKDILPTSPRSESAFGRRLSFSATPLQRPSRFKPAAPDGYAPSVSGDDFVPAAPIYPYPSRISPSLQQLLSQSSFKQKHILSVSQFTRPDLHLLFTVAQEMRLGVQRHGVLDILKGRVLTTLFFEPSTRTSASFNAAMQRLGGRTIPVATEHSSTQKGETLQDTVRTLGCYSDAIVLRHPDDSSAATAAKFSPVPIINGGNGAMEHPTQAFLDLFTIREELGTVNDITITFTGDLKYGRTVHSLVKLLQFYEVRVQLVSPKALAVPEEVRQQLVSSGQLILESTELTPQIIARSDVLYCTRVQKERFEDLGEYERLKNAFVIDNTVLKHAKSQMIVMHPLPRNQEVCEEVDFDQRAAYFRQMKYGLYCRMALLALVMAS

Target ORF information:

RefSeq Version	XM_002628680.1
Organism	Blastomyces gilchristii SLH14081
Definition	Ajellomyces dermatitidis SLH14081 bifunctional pyrimidine biosynthesis protein pyrABCN, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002628680.1

ORF Insert Sequence:

ATGCCGCTTC TCAATGCCGC TGGGGATGCC AAAAGCTCTC CTGGCCAGTC TCATTCCGTC 
AGGAGCAGAC GACTAACCGA TACTACTGTT ACTCCGTCCA GCCCTGCTTC TGGTGGCGTC 
GTCGCATACA CCACCCAGAA TAAAGACCTG CCTTCCCTCG CTCCCCAAAC GGCATATTCC 
CTCAATGGCG GTGCTATCCC CCCCGCCTCT GCCCCAATGG CTGGCGAAGG GGGAGATGGG 
AGCAAGTGGT ATGCCCTAGA GCTGGAGGAT GGCACCGTCT ACCAGGGTTA CAGTTTTGGT 
GCCGAAGCGA GCATTGCTGG CGAGTTGGTT TTTCAAACTG GCATGGTGGG CTACCCAGAA 
TCAATCACAG ATCCTTCCTA CCGTGGCCAG ATCTTGATCA TCACCTTCCC GCTGGTAGGC 
AACTATGGCG TCCCTTCGCG AGAAACGGTT GATGAGCTCC TCGGGCAAGA TATGCCCAAA 
TATTTCGAGT CCAGTGAGAT CCATGTTGCT GGTCTTGTAG TGGCCAATTA CTGCGGAGAG 
GACTACTCGC ACTTCTTGGC CGAGTCGTCT CTCGGGAAGT GGTTGAAGGA ACAAGGAGTT 
CCTGCCATGT ATGGTGTTGA TACCCGCGCC CTCACAAAGA GAATTCGAGA GAAGGGAAGC 
ATGCTTGGCC GCATGCTTTT GCAGAAACCC GGTGTGAGTG TTGAAACCTT GAATAGCGAC 
GCGCCGAAAA ACGGCTCGAC TACTGCTGTT GTTGATGACG AAGAGAAATC CGATCTTTGG 
AGAGCATCCT TTGAACAAAT TGAATGGATG GACCCTGATA AGAGGAACCT GGTCAACGAT 
GTCTCTATTA GGAAACCCCA GCTGTTCTCC CCACCAAAGG ATTCGGTCCT TTCTCACCCC 
TGTGGTCGCC CGATCAGAGT TGTGTGCGTT GACGTTGGTA TGAAATATAA CCAACTACGG 
TGCCTTCTCG CTCGCGGCGT CGAGCTCCTT GTTGTACCAT GGGATTATGA TTTCCCCAGC 
TTGGCAGGAA AGGAATATGA CGGCCTTTTC CTTTCCAACG GCCCGGGCAA CCCCGCCACC 
CTGTCCAAAA CAGTGGAGAA TATTAGAACC GCCATCAAGG ACTCGCGCAC TCCAATCTTT 
GGTATTTGTC TTGGACACCA GTTACTTGCC CGGGCTGCGG GTGCGTCTAC CATTAAGATG 
AAGTTCGGTA ACCGAGGACA AAATATCCCC TGTACCAGCC TTATCTCCGG TCGCTGTTAC 
ATTACTTCGC AAAACCACGG ATATGCAGTT GATTCTATGA CATTGCCAGA GGGATGGGAG 
GAGCTCTTTG TCAACGCTAA TGATGGAAGC AACGAAGGTA TCAGACACGT TAGCCGCCCA 
TTTTTCAGTG TCCAATTCCA CCCAGAGAGC GCCCCAGGAC CCCAGGACAC AGAGTTCCTA 
TTCGATGTCT TCATCGATGC TATTAAAACG AACGTGGCAT TCCCTAGTGC CTTAGAGAAG 
CCCGTTAGCT TCCCTGGCGC CACCGCTAAA GAGAACGAGC TTGCAAACCC GAGAGTGAGC 
GCTAAGAAAG CCCTTATCCT AGGTAGCGGT GGTCTCAGTA TCGGACAAGC TGGAGAGTTT 
GACTATTCTG GAAGTCAAGC CATAAAGGCT TTGAAAGAAG AAGGAATTTA CACCGTCCTT 
ATTAACCCCA ATATCGCGAC GATTCAGACT TCCAAGGGCC TTGCAGATAA GGTCTATTTC 
TTACCAGTCA ACGCAGACTT TGTCCGCAAG GTTATCAAAC ACGAACGGCC AGATGCCATC 
TATGTAACAT TTGGCGGCCA AACTGCGCTC CAGGTTGGTA TTCAGCTCAA AGATGAGTTC 
GAGGAACTTG GCGTTCGAGT TCTTGGTACT CCGATAGACA CCATCATTAC GACAGAAGAC 
CGCGAGTTAT TCGCGAGAAG CATGGAAGCC CTTGGTGAGA AGTGCGCCAA ATCCGCCTCT 
GCTTCCACGC TCGAGGAAGC CATGGAAGTT GTCAAGGATA TTGGATTTCC CGTCATTGTC 
CGTGCTGCGT ACGCTCTTGG TGGCCTTGGA AGCGGTTTCG CCGAGGATCC TGAGCAGCTG 
CGGGAGCTGT GCACAAAAGC CTTCGCTGCA AGCCCACAGG TTCTTATTGA GAGGAGTATG 
AAAGGGTGGA AGGAGATTGA ATATGAAGTG GTCCGCGATG CTCGTGATAA CTGCATCACT 
GTGTGTAACA TGGAGAATTT TGATCCGCTT GGTATCCACA CTGGCGATTC TATTGTCGTC 
GCCCCGTCGC AAACCCTTTC TGATGAGGAC TACAATATGC TTCGAACGAC TGCTATCAAT 
GTCATCCGCC ATCTGGGTGT GGTGGGTGAA TGTAACATTC AATATGCATT GAACCCATTC 
TCAAAAGAGT ATTGCATTAT TGAGGTCAAT GCTCGCCTGT CAAGATCATC TGCACTCGCA 
TCTAAAGCAA CAGGCTATCC CCTCGCTTTC GTCGCGGCTA AACTTGGCCT CGGAATCCCG 
CTGAATGAGA TTTCAAACTC AGTCACCAAA GTCACATGTG CCTGCTTTGA ACCTTCTCTT 
GATTATGTCG TCGTTAAGAT TCCCCGCTGG GATCTCAAGA AGTTTACCCG TGTCTCCACG 
CAGCTCGGGT CTTCAATGAA GAGTGTTGGG GAGGTTATGA GCATTGGACG GACTTTTGAG 
GAGGCCATTC AGAAAGCCAT ACGTTCTGTC GACTTTCACA ATCTTGGGTT CAACGAGACC 
AACGCACTCA TGTCGATTAA GGCTGAGCTT CAAACACCTT CCGATCAGCG ACTGTTCGCT 
ATCGCCAACG CCATGCATTC GGGCTACACT GTCGACGATA TCTGGGAATT GACCAAAATC 
GACAAGTGGT TCCTCCGAAA ATTGAAAGGC CTCAGCGATT TTGGAAAACT AATGTCTGGT 
TACAACGCCA CTACAGCACC CATTTCTCTC ATTCGACAGG CGAAGCAGCT CGGTTTCTCG 
GACCGGCAAC TGGCGAAGTT TTTGAGCTCC AATGAGTTGG CTATCAGGCG ACTGAGAGTG 
GAAGCAGGCA TTGTGCCCGT GGTCAAACAA ATTGATACCG TCGCTGCTGA ATTCCCGGCA 
TTCACGAACT ACTTGTATCT GACATACAAC GGCTCCGAAA ACGATGTGGA CTTTAACGAC 
CAAGGTGTTA TGGTTCTTGG CTCTGGTGTC TACAGAATTG GATCCTCTGT CGAGTTTGAT 
TGGTGTTCTG TGCGAGCCAT TCGAACTCTT CGAGAGCAGG GTTTTAAGAC GGTGATGGTG 
AATGTTCACA ATCTCCTAGA TTTCACCATC TCCTATATTT CTCAAAAGCT CTGCAAGATT 
TCCCAAAAGC TCCCCAAGGT CCTCTGCTGC TCTGCCTCCA CCAGTTTGTT TCCTGGTTGC 
TTGCTCCTGA GACGATTCGA TTTCTATGGC TTCTCCTGTG TTCTCTGCTC CGACGGTATT 
CAAGCTCATC AGCTTGTTGC TTGGAAATGC CTTTGTGATC CCCGTCCACG CTTCAAACAT 
CCTCTATTTG CAACTTCTAT AGCCAGGAAG AGCTGGAGGC AATTGCTGAG GGGATCTCTC 
TCTCTCTCCT TTGTCTCCGG CATTTTTGAC CTGCTGAAAG CCCCAAACCC CGCCCTTCGA 
GGCCCGCAAC GCCAACTGGG ACGCCCACAT ACTTTGGGCC GCCTGATGTT TCTAATTCTC 
GAAGCCACCC TTGCACATAT TCTGGGCGAT GAGTGCGAAA ATGGAAACTA CAGGATGGGC 
CATATCTGTT TTTGGGATCG CGAAAAACTC GAATATGACG GCGTCTGCAG TCATTGTGCT 
TTGGACGAAG GCATTCGCGG CGAGTTCGAC CTGTCTCCTG AGGAATATGA AATATATGCA 
AAAGAACGCG AAATCAAGCA TCGTGAAATC CACCGGGACG GCAATTCAAG CTGGCATTAT 
AAGCAGATGG CAGGGAACTA TCCGGATTAT ATTGAGGCAG CCCATGAGCG ATCCAACCCC 
GAGACTCTCA GCACAGATTA CGACGAAGCA GACCGACTGT ATTTTGAAAA TATCGATTTG 
GAGTCCGTCC TTGATGTCTA CCAACTTGAG TCTGCAAGCG GGGTTATTGT ATCTATGGGA 
GGACAGGCAC CGAGCAACAT CGCTCTTCCC TTACACAGAC TCCATGTTAA TGTCCTTGGT 
ACATCTCCGG AGATGATTGA TACAGCTGAG AATCGGTACA AATTTTCGCG ACTCCTTGAC 
CATATTGAGG TCGATCAGCC AGCTTGGAAG GAATTGACCA GTATCGAGGA GGCCACCGAA 
TTCTGTGCGA AGGTTGGATA CCCAGTACTG GTGCGGCCTT CGTACGTGCT CTCTGGTGCT 
GCCATGAACA CGGTTTATTC TTTGGATGAT CTTGCAAGCT ACCTCAACCA GGCTGTTGAG 
GTCTCCCGTG ATCACCCTGT GGTGATTACC AAATACATAG AGAACGCCAA GGAAATTGAA 
ATGGACGCAG TTGCCAAGAA TGGTGTTATG GTCGGCCATT TTATTTCAGA ACATGTTGAG 
AATGCGGGTG TCCATTCTGG CGATGCTACT TTGATTCTTC CGCCCCAGGA CTTGGACCCG 
GAAACCGTCC GGCGAATCGA GGAAGCGACC CGCAAGATCG GCAATGCCCT GAACGTCACT 
GGACCTTTCA ATATCCAGTT CATCGCCAAA GATAACGACA TTAAAGTTAT CGAGTGCAAT 
GTCAGAGCAT CTCGATCCTT CCCGTTCGTT TCCAAAGTCA TGGGAGTTGA CTTGATCGAA 
ATGGCAACGA TGGCAATGAT TGGCAAGCCG TTCACTGAGT ACCCGCCGGT AACCATTCCC 
AAGAACTACG TCGGTGTCAA GGTTCCGCAA TTCTCATTCT CCCGCCTCTC TGGTGCTGAC 
CCCGTGCTCG GAGTGGAGAT GGCTTCTACC GGTGAAGTTG CTTGCTTCGG TCGTGACAGG 
TATGAGGCAT ATCTCAAAGC TCTGATTTCG ACTGGCTTCA AGCTTCCGAA AAAGAACGTC 
CTGTTCTCCA TCGGTTCCTT CAAGGAGAAG GTAGAGATGT TGCCGTCTAT CAGAAAGCTG 
CACGCGCTTG GCTACAACCT TTTTGCCACG GCGGGCACTG CGGACTATCT CAAAGAGCAC 
GGACTTCCGG TCAAATACCT CGAAGTTCTT GCGGGAGAGG AGGAGGACCT TAAGTCTGAA 
TATTCTCTTA CGCAGCATTT GGCCAACAAT CTGATTGATC TTTACATCAA TCTTCCCTCC 
AGCAATAGGT TCCGGCGGCC TGCAAACTAC ATGAGCAAGG GCTATCGAAC ACGGCGAATG 
GCAGTCGACT ACCAGACCCC GCTAGTCACC AACGTTAAGA ACGCGAAAAT CCTGATCGAA 
GCGATTGCAC GCCATTACAC TATGAACGTG ATGAGCATTG ATTGCCAAAC GAGCTATCGC 
GCTGTTACTA TCCCCGGCCT AATCAACGTC GCAGCGTTCG TGCCAGGACT CGCTAACCCA 
GATTCTCCAG ATTTCCAGGT GGTGACTAAG GCTTCCATTG CGAACGGTTT TAGCATGGTC 
CGCATCATGC CCGTGGGCGT GGATAGCTTC GTTACTGACT CTCAGGCGTT GAGAACGGCC 
CAACATAATT CGTCAAAGGG ATCGTACTGC GATTTCAACC TCTCTGTCAC GGCAACATCT 
AATAATTCAG ATAAGCTCGG CCTAGTCACC AGTGAAGTCG GTTCGTTATT TATCCCATTC 
AACCACCTAT CAGGCAACAT CAACAAGGTG GCAACGGTTA CTTCGCACTT TGCATCATGG 
CCGACCAATA AACCTATAAT CACCGATGCC AAAGCTACGG ACCTGGCTTC CATTTTGCTG 
CTTGCCAGTC TTCACAATCG CAATATCCAC GTCATGAGCG TTACTAGCAA AGAGGACATG 
GGCCTGATTG CGCTTAGCAA GGAAAAGGGT CTGAAAGTGA CTTGCGATGT TGCAATCTAC 
TCCCTTTTCC TGTCTACCGA TGATTACCCT GGTTGCGCAT GCCTTCCTTC GAAGAAAGAC 
CAAGAAGTGC TCTGGGAGCA CTTGGATACG ATTGATATAT TTTCTATCGG TAGTCTTCCA 
TACCAGGTTG CGGGTAGCGA TGCTTCTCCC GCTGTTGGGA TCCAGGATGC GCTTCCTCTG 
CTCTTCACAG CAGTTTCTGA CAGGCGACTA ACTATCGAGG ATGTTACCGC CAGACTCCAC 
GATAACCCGA AGAGGATCTT TGAACTTCAT GACCAGCCAG ATGCCTCCGT GGAGATCGAG 
TTTGACAGGC CATATGTATT CCAGAGCCAT GATGTCTGGT CACCATTTGC CGGTAAGACT 
CTAAAGGCAT CCGTCCGACG AGTTACATTC CAGGGGAAGA CCTCTTGCTT GGACGGTGAA 
ATGATGCTGG ATGCACCGAA GGGGGCCGAC ATGTCCTCTC ATCGGTTAGC GCCACCGGCT 
CCAGCATCGC CGGCAGTCAA AGACATTCTT CCCACCTCTC CTCGATCGGA AAGTGCCTTT 
GGGAGACGTT TGTCTTTCTC GGCCACACCG CTTCAGCGCC CGTCGAGATT CAAACCTGCT 
GCTCCTGATG GATATGCTCC ATCTGTCTCA GGAGACGACT TTGTGCCTGC CGCTCCGATT 
TACCCATATC CGTCCCGGAT TTCACCCTCA TTGCAACAGC TGCTTTCACA ATCGTCTTTC 
AAGCAGAAGC ATATCCTATC TGTCAGCCAG TTCACCCGCC CAGATCTTCA CCTTCTATTC 
ACGGTTGCGC AAGAGATGAG GCTGGGTGTC CAACGCCATG GTGTCCTTGA CATCCTGAAG 
GGCCGCGTCC TCACAACCCT CTTCTTCGAG CCCTCCACTC GTACGTCTGC TTCGTTCAAT 
GCCGCTATGC AACGACTGGG TGGCCGCACC ATCCCCGTCG CGACTGAGCA TTCGTCCACT 
CAAAAGGGCG AAACCCTTCA GGACACTGTA CGCACCCTGG GCTGCTACAG CGACGCGATC 
GTCCTCCGTC ATCCGGATGA CAGCAGCGCT GCCACCGCCG CCAAATTCTC TCCAGTTCCC 
ATCATCAACG GCGGAAACGG AGCCATGGAA CACCCCACCC AAGCCTTCCT CGACCTCTTC 
ACCATCCGCG AGGAACTCGG CACCGTCAAC GACATCACAA TTACCTTTAC CGGCGATTTA 
AAATACGGCC GCACCGTGCA CTCCCTCGTC AAACTCCTCC AATTCTACGA AGTTCGCGTC 
CAGCTCGTCT CGCCCAAGGC GCTCGCTGTC CCCGAGGAGG TCCGTCAACA GCTCGTCTCG 
TCCGGCCAGC TGATCCTGGA GTCGACGGAG CTTACTCCGC AGATCATCGC ACGGTCGGAT 
GTGCTGTACT GCACCCGGGT GCAGAAGGAG CGGTTCGAGG ACTTGGGCGA GTATGAGCGG 
TTGAAGAATG CATTCGTCAT CGATAACACA GTCCTTAAGC ATGCGAAGAG CCAGATGATC 
GTTATGCATC CTCTGCCGCG CAACCAGGAG GTCTGCGAGG AGGTCGATTT TGACCAGCGC 
GCGGCTTATT TCCGACAGAT GAAATATGGG CTCTACTGTC GCATGGCATT ACTGGCATTG 
GTCATGGCAT CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BDBG_01634 ( XM_031720420.1 ) cDNA ORF clone, Blastomyces gilchristii SLH14081 -> XP_031576597.1 Blastomyces gilchristii SLH14081 protein pyrABCN (BDBG_01634), mRNA.

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CloneID	OAl116695
Clone ID Related Accession (Same CDS sequence)	XM_031720420.1
Accession Version	XM_031720420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574697600000
Organism	Blastomyces gilchristii SLH14081
Product	protein pyrABCN
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101681).

ATGCCGCTTC TCAATGCCGC TGGGGATGCC AAAAGCTCTC CTGGCCAGTC TCATTCCGTC 
AGGAGCAGAC GACTAACCGA TACTACTGTT ACTCCGTCCA GCCCTGCTTC TGGTGGCGTC 
GTCGCATACA CCACCCAGAA TAAAGACCTG CCTTCCCTCG CTCCCCAAAC GGCATATTCC 
CTCAATGGCG GTGCTATCCC CCCCGCCTCT GCCCCAATGG CTGGCGAAGG GGGAGATGGG 
AGCAAGTGGT ATGCCCTAGA GCTGGAGGAT GGCACCGTCT ACCAGGGTTA CAGTTTTGGT 
GCCGAAGCGA GCATTGCTGG CGAGTTGGTT TTTCAAACTG GCATGGTGGG CTACCCAGAA 
TCAATCACAG ATCCTTCCTA CCGTGGCCAG ATCTTGATCA TCACCTTCCC GCTGGTAGGC 
AACTATGGCG TCCCTTCGCG AGAAACGGTT GATGAGCTCC TCGGGCAAGA TATGCCCAAA 
TATTTCGAGT CCAGTGAGAT CCATGTTGCT GGTCTTGTAG TGGCCAATTA CTGCGGAGAG 
GACTACTCGC ACTTCTTGGC CGAGTCGTCT CTCGGGAAGT GGTTGAAGGA ACAAGGAGTT 
CCTGCCATGT ATGGTGTTGA TACCCGCGCC CTCACAAAGA GAATTCGAGA GAAGGGAAGC 
ATGCTTGGCC GCATGCTTTT GCAGAAACCC GGTGTGAGTG TTGAAACCTT GAATAGCGAC 
GCGCCGAAAA ACGGCTCGAC TACTGCTGTT GTTGATGACG AAGAGAAATC CGATCTTTGG 
AGAGCATCCT TTGAACAAAT TGAATGGATG GACCCTGATA AGAGGAACCT GGTCAACGAT 
GTCTCTATTA GGAAACCCCA GCTGTTCTCC CCACCAAAGG ATTCGGTCCT TTCTCACCCC 
TGTGGTCGCC CGATCAGAGT TGTGTGCGTT GACGTTGGTA TGAAATATAA CCAACTACGG 
TGCCTTCTCG CTCGCGGCGT CGAGCTCCTT GTTGTACCAT GGGATTATGA TTTCCCCAGC 
TTGGCAGGAA AGGAATATGA CGGCCTTTTC CTTTCCAACG GCCCGGGCAA CCCCGCCACC 
CTGTCCAAAA CAGTGGAGAA TATTAGAACC GCCATCAAGG ACTCGCGCAC TCCAATCTTT 
GGTATTTGTC TTGGACACCA GTTACTTGCC CGGGCTGCGG GTGCGTCTAC CATTAAGATG 
AAGTTCGGTA ACCGAGGACA AAATATCCCC TGTACCAGCC TTATCTCCGG TCGCTGTTAC 
ATTACTTCGC AAAACCACGG ATATGCAGTT GATTCTATGA CATTGCCAGA GGGATGGGAG 
GAGCTCTTTG TCAACGCTAA TGATGGAAGC AACGAAGGTA TCAGACACGT TAGCCGCCCA 
TTTTTCAGTG TCCAATTCCA CCCAGAGAGC GCCCCAGGAC CCCAGGACAC AGAGTTCCTA 
TTCGATGTCT TCATCGATGC TATTAAAACG AACGTGGCAT TCCCTAGTGC CTTAGAGAAG 
CCCGTTAGCT TCCCTGGCGC CACCGCTAAA GAGAACGAGC TTGCAAACCC GAGAGTGAGC 
GCTAAGAAAG CCCTTATCCT AGGTAGCGGT GGTCTCAGTA TCGGACAAGC TGGAGAGTTT 
GACTATTCTG GAAGTCAAGC CATAAAGGCT TTGAAAGAAG AAGGAATTTA CACCGTCCTT 
ATTAACCCCA ATATCGCGAC GATTCAGACT TCCAAGGGCC TTGCAGATAA GGTCTATTTC 
TTACCAGTCA ACGCAGACTT TGTCCGCAAG GTTATCAAAC ACGAACGGCC AGATGCCATC 
TATGTAACAT TTGGCGGCCA AACTGCGCTC CAGGTTGGTA TTCAGCTCAA AGATGAGTTC 
GAGGAACTTG GCGTTCGAGT TCTTGGTACT CCGATAGACA CCATCATTAC GACAGAAGAC 
CGCGAGTTAT TCGCGAGAAG CATGGAAGCC CTTGGTGAGA AGTGCGCCAA ATCCGCCTCT 
GCTTCCACGC TCGAGGAAGC CATGGAAGTT GTCAAGGATA TTGGATTTCC CGTCATTGTC 
CGTGCTGCGT ACGCTCTTGG TGGCCTTGGA AGCGGTTTCG CCGAGGATCC TGAGCAGCTG 
CGGGAGCTGT GCACAAAAGC CTTCGCTGCA AGCCCACAGG TTCTTATTGA GAGGAGTATG 
AAAGGGTGGA AGGAGATTGA ATATGAAGTG GTCCGCGATG CTCGTGATAA CTGCATCACT 
GTGTGTAACA TGGAGAATTT TGATCCGCTT GGTATCCACA CTGGCGATTC TATTGTCGTC 
GCCCCGTCGC AAACCCTTTC TGATGAGGAC TACAATATGC TTCGAACGAC TGCTATCAAT 
GTCATCCGCC ATCTGGGTGT GGTGGGTGAA TGTAACATTC AATATGCATT GAACCCATTC 
TCAAAAGAGT ATTGCATTAT TGAGGTCAAT GCTCGCCTGT CAAGATCATC TGCACTCGCA 
TCTAAAGCAA CAGGCTATCC CCTCGCTTTC GTCGCGGCTA AACTTGGCCT CGGAATCCCG 
CTGAATGAGA TTTCAAACTC AGTCACCAAA GTCACATGTG CCTGCTTTGA ACCTTCTCTT 
GATTATGTCG TCGTTAAGAT TCCCCGCTGG GATCTCAAGA AGTTTACCCG TGTCTCCACG 
CAGCTCGGGT CTTCAATGAA GAGTGTTGGG GAGGTTATGA GCATTGGACG GACTTTTGAG 
GAGGCCATTC AGAAAGCCAT ACGTTCTGTC GACTTTCACA ATCTTGGGTT CAACGAGACC 
AACGCACTCA TGTCGATTAA GGCTGAGCTT CAAACACCTT CCGATCAGCG ACTGTTCGCT 
ATCGCCAACG CCATGCATTC GGGCTACACT GTCGACGATA TCTGGGAATT GACCAAAATC 
GACAAGTGGT TCCTCCGAAA ATTGAAAGGC CTCAGCGATT TTGGAAAACT AATGTCTGGT 
TACAACGCCA CTACAGCACC CATTTCTCTC ATTCGACAGG CGAAGCAGCT CGGTTTCTCG 
GACCGGCAAC TGGCGAAGTT TTTGAGCTCC AATGAGTTGG CTATCAGGCG ACTGAGAGTG 
GAAGCAGGCA TTGTGCCCGT GGTCAAACAA ATTGATACCG TCGCTGCTGA ATTCCCGGCA 
TTCACGAACT ACTTGTATCT GACATACAAC GGCTCCGAAA ACGATGTGGA CTTTAACGAC 
CAAGGTGTTA TGGTTCTTGG CTCTGGTGTC TACAGAATTG GATCCTCTGT CGAGTTTGAT 
TGGTGTTCTG TGCGAGCCAT TCGAACTCTT CGAGAGCAGG GTTTTAAGAC GGTGATGGTG 
AATTCCAACC CCGAGACTCT CAGCACAGAT TACGACGAAG CAGACCGACT GTATTTTGAA 
AATATCGATT TGGAGTCCGT CCTTGATGTC TACCAACTTG AGTCTGCAAG CGGGGTTATT 
GTATCTATGG GAGGACAGGC ACCGAGCAAC ATCGCTCTTC CCTTACACAG ACTCCATGTT 
AATGTCCTTG GTACATCTCC GGAGATGATT GATACAGCTG AGAATCGGTA CAAATTTTCG 
CGACTCCTTG ACCATATTGA GGTCGATCAG CCAGCTTGGA AGGAATTGAC CAGTATCGAG 
GAGGCCACCG AATTCTGTGC GAAGGTTGGA TACCCAGTAC TGGTGCGGCC TTCGTACGTG 
CTCTCTGGTG CTGCCATGAA CACGGTTTAT TCTTTGGATG ATCTTGCAAG CTACCTCAAC 
CAGGCTGTTG AGGTCTCCCG TGATCACCCT GTGGTGATTA CCAAATACAT AGAGAACGCC 
AAGGAAATTG AAATGGACGC AGTTGCCAAG AATGGTGTTA TGGTCGGCCA TTTTATTTCA 
GAACATGTTG AGAATGCGGG TGTCCATTCT GGCGATGCTA CTTTGATTCT TCCGCCCCAG 
GACTTGGACC CGGAAACCGT CCGGCGAATC GAGGAAGCGA CCCGCAAGAT CGGCAATGCC 
CTGAACGTCA CTGGACCTTT CAATATCCAG TTCATCGCCA AAGATAACGA CATTAAAGTT 
ATCGAGTGCA ATGTCAGAGC ATCTCGATCC TTCCCGTTCG TTTCCAAAGT CATGGGAGTT 
GACTTGATCG AAATGGCAAC GATGGCAATG ATTGGCAAGC CGTTCACTGA GTACCCGCCG 
GTAACCATTC CCAAGAACTA CGTCGGTGTC AAGGTTCCGC AATTCTCATT CTCCCGCCTC 
TCTGGTGCTG ACCCCGTGCT CGGAGTGGAG ATGGCTTCTA CCGGTGAAGT TGCTTGCTTC 
GGTCGTGACA GGTATGAGGC ATATCTCAAA GCTCTGATTT CGACTGGCTT CAAGCTTCCG 
AAAAAGAACG TCCTGTTCTC CATCGGTTCC TTCAAGGAGA AGGTAGAGAT GTTGCCGTCT 
ATCAGAAAGC TGCACGCGCT TGGCTACAAC CTTTTTGCCA CGGCGGGCAC TGCGGACTAT 
CTCAAAGAGC ACGGACTTCC GGTCAAATAC CTCGAAGTTC TTGCGGGAGA GGAGGAGGAC 
CTTAAGTCTG AATATTCTCT TACGCAGCAT TTGGCCAACA ATCTGATTGA TCTTTACATC 
AATCTTCCCT CCAGCAATAG GTTCCGGCGG CCTGCAAACT ACATGAGCAA GGGCTATCGA 
ACACGGCGAA TGGCAGTCGA CTACCAGACC CCGCTAGTCA CCAACGTTAA GAACGCGAAA 
ATCCTGATCG AAGCGATTGC ACGCCATTAC ACTATGAACG TGATGAGCAT TGATTGCCAA 
ACGAGCTATC GCGCTGTTAC TATCCCCGGC CTAATCAACG TCGCAGCGTT CGTGCCAGGA 
CTCGCTAACC CAGATTCTCC AGATTTCCAG GTGGTGACTA AGGCTTCCAT TGCGAACGGT 
TTTAGCATGG TCCGCATCAT GCCCGTGGGC GTGGATAGCT TCGTTACTGA CTCTCAGGCG 
TTGAGAACGG CCCAACATAA TTCGTCAAAG GGATCGTACT GCGATTTCAA CCTCTCTGTC 
ACGGCAACAT CTAATAATTC AGATAAGCTC GGCCTAGTCA CCAGTGAAGT CGGTTCGTTA 
TTTATCCCAT TCAACCACCT ATCAGGCAAC ATCAACAAGG TGGCAACGGT TACTTCGCAC 
TTTGCATCAT GGCCGACCAA TAAACCTATA ATCACCGATG CCAAAGCTAC GGACCTGGCT 
TCCATTTTGC TGCTTGCCAG TCTTCACAAT CGCAATATCC ACGTCATGAG CGTTACTAGC 
AAAGAGGACA TGGGCCTGAT TGCGCTTAGC AAGGAAAAGG GTCTGAAAGT GACTTGCGAT 
GTTGCAATCT ACTCCCTTTT CCTGTCTACC GATGATTACC CTGGTTGCGC ATGCCTTCCT 
TCGAAGAAAG ACCAAGAAGT GCTCTGGGAG CACTTGGATA CGATTGATAT ATTTTCTATC 
GGTAGTCTTC CATACCAGGT TGCGGGTAGC GATGCTTCTC CCGCTGTTGG GATCCAGGAT 
GCGCTTCCTC TGCTCTTCAC AGCAGTTTCT GACAGGCGAC TAACTATCGA GGATGTTACC 
GCCAGACTCC ACGATAACCC GAAGAGGATC TTTGAACTTC ATGACCAGCC AGATGCCTCC 
GTGGAGATCG AGTTTGACAG GCCATATGTA TTCCAGAGCC ATGATGTCTG GTCACCATTT 
GCCGGTAAGA CTCTAAAGGC ATCCGTCCGA CGAGTTACAT TCCAGGGGAA GACCTCTTGC 
TTGGACGGTG AAATGATGCT GGATGCACCG AAGGGGGCCG ACATGTCCTC TCATCGGTTA 
GCGCCACCGG CTCCAGCATC GCCGGCAGTC AAAGACATTC TTCCCACCTC TCCTCGATCG 
GAAAGTGCCT TTGGGAGACG TTTGTCTTTC TCGGCCACAC CGCTTCAGCG CCCGTCGAGA 
TTCAAACCTG CTGCTCCTGA TGGATATGCT CCATCTGTCT CAGGAGACGA CTTTGTGCCT 
GCCGCTCCGA TTTACCCATA TCCGTCCCGG ATTTCACCCT CATTGCAACA GCTGCTTTCA 
CAATCGTCTT TCAAGCAGAA GCATATCCTA TCTGTCAGCC AGTTCACCCG CCCAGATCTT 
CACCTTCTAT TCACGGTTGC GCAAGAGATG AGGCTGGGTG TCCAACGCCA TGGTGTCCTT 
GACATCCTGA AGGGCCGCGT CCTCACAACC CTCTTCTTCG AGCCCTCCAC TCGTACGTCT 
GCTTCGTTCA ATGCCGCTAT GCAACGACTG GGTGGCCGCA CCATCCCCGT CGCGACTGAG 
CATTCGTCCA CTCAAAAGGG CGAAACCCTT CAGGACACTG TACGCACCCT GGGCTGCTAC 
AGCGACGCGA TCGTCCTCCG TCATCCGGAT GACAGCAGCG CTGCCACCGC CGCCAAATTC 
TCTCCAGTTC CCATCATCAA CGGCGGAAAC GGAGCCATGG AACACCCCAC CCAAGCCTTC 
CTCGACCTCT TCACCATCCG CGAGGAACTC GGCACCGTCA ACGACATCAC AATTACCTTT 
ACCGGCGATT TAAAATACGG CCGCACCGTG CACTCCCTCG TCAAACTCCT CCAATTCTAC 
GAAGTTCGCG TCCAGCTCGT CTCGCCCAAG GCGCTCGCTG TCCCCGAGGA GGTCCGTCAA 
CAGCTCGTCT CGTCCGGCCA GCTGATCCTG GAGTCGACGG AGCTTACTCC GCAGATCATC 
GCACGGTCGG ATGTGCTGTA CTGCACCCGG GTGCAGAAGG AGCGGTTCGA GGACTTGGGC 
GAGTATGAGC GGTTGAAGAA TGCATTCGTC ATCGATAACA CAGTCCTTAA GCATGCGAAG 
AGCCAGATGA TCGTTATGCA TCCTCTGCCG CGCAACCAGG AGGTCTGCGA GGAGGTCGAT 
TTTGACCAGC GCGCGGCTTA TTTCCGACAG ATGAAATATG GGCTCTACTG TCGCATGGCA 
TTACTGGCAT TGGTCATGGC ATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031576597.1
CDS	193..7119
Translation	MPLLNAAGDAKSSPGQSHSVRSRRLTDTTVTPSSPASGGVVAYTTQNKDLPSLAPQTAYSLNGGAIPPASAPMAGEGGDGSKWYALELEDGTVYQGYSFGAEASIAGELVFQTGMVGYPESITDPSYRGQILIITFPLVGNYGVPSRETVDELLGQDMPKYFESSEIHVAGLVVANYCGEDYSHFLAESSLGKWLKEQGVPAMYGVDTRALTKRIREKGSMLGRMLLQKPGVSVETLNSDAPKNGSTTAVVDDEEKSDLWRASFEQIEWMDPDKRNLVNDVSIRKPQLFSPPKDSVLSHPCGRPIRVVCVDVGMKYNQLRCLLARGVELLVVPWDYDFPSLAGKEYDGLFLSNGPGNPATLSKTVENIRTAIKDSRTPIFGICLGHQLLARAAGASTIKMKFGNRGQNIPCTSLISGRCYITSQNHGYAVDSMTLPEGWEELFVNANDGSNEGIRHVSRPFFSVQFHPESAPGPQDTEFLFDVFIDAIKTNVAFPSALEKPVSFPGATAKENELANPRVSAKKALILGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTVLINPNIATIQTSKGLADKVYFLPVNADFVRKVIKHERPDAIYVTFGGQTALQVGIQLKDEFEELGVRVLGTPIDTIITTEDRELFARSMEALGEKCAKSASASTLEEAMEVVKDIGFPVIVRAAYALGGLGSGFAEDPEQLRELCTKAFAASPQVLIERSMKGWKEIEYEVVRDARDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAINVIRHLGVVGECNIQYALNPFSKEYCIIEVNARLSRSSALASKATGYPLAFVAAKLGLGIPLNEISNSVTKVTCACFEPSLDYVVVKIPRWDLKKFTRVSTQLGSSMKSVGEVMSIGRTFEEAIQKAIRSVDFHNLGFNETNALMSIKAELQTPSDQRLFAIANAMHSGYTVDDIWELTKIDKWFLRKLKGLSDFGKLMSGYNATTAPISLIRQAKQLGFSDRQLAKFLSSNELAIRRLRVEAGIVPVVKQIDTVAAEFPAFTNYLYLTYNGSENDVDFNDQGVMVLGSGVYRIGSSVEFDWCSVRAIRTLREQGFKTVMVNSNPETLSTDYDEADRLYFENIDLESVLDVYQLESASGVIVSMGGQAPSNIALPLHRLHVNVLGTSPEMIDTAENRYKFSRLLDHIEVDQPAWKELTSIEEATEFCAKVGYPVLVRPSYVLSGAAMNTVYSLDDLASYLNQAVEVSRDHPVVITKYIENAKEIEMDAVAKNGVMVGHFISEHVENAGVHSGDATLILPPQDLDPETVRRIEEATRKIGNALNVTGPFNIQFIAKDNDIKVIECNVRASRSFPFVSKVMGVDLIEMATMAMIGKPFTEYPPVTIPKNYVGVKVPQFSFSRLSGADPVLGVEMASTGEVACFGRDRYEAYLKALISTGFKLPKKNVLFSIGSFKEKVEMLPSIRKLHALGYNLFATAGTADYLKEHGLPVKYLEVLAGEEEDLKSEYSLTQHLANNLIDLYINLPSSNRFRRPANYMSKGYRTRRMAVDYQTPLVTNVKNAKILIEAIARHYTMNVMSIDCQTSYRAVTIPGLINVAAFVPGLANPDSPDFQVVTKASIANGFSMVRIMPVGVDSFVTDSQALRTAQHNSSKGSYCDFNLSVTATSNNSDKLGLVTSEVGSLFIPFNHLSGNINKVATVTSHFASWPTNKPIITDAKATDLASILLLASLHNRNIHVMSVTSKEDMGLIALSKEKGLKVTCDVAIYSLFLSTDDYPGCACLPSKKDQEVLWEHLDTIDIFSIGSLPYQVAGSDASPAVGIQDALPLLFTAVSDRRLTIEDVTARLHDNPKRIFELHDQPDASVEIEFDRPYVFQSHDVWSPFAGKTLKASVRRVTFQGKTSCLDGEMMLDAPKGADMSSHRLAPPAPASPAVKDILPTSPRSESAFGRRLSFSATPLQRPSRFKPAAPDGYAPSVSGDDFVPAAPIYPYPSRISPSLQQLLSQSSFKQKHILSVSQFTRPDLHLLFTVAQEMRLGVQRHGVLDILKGRVLTTLFFEPSTRTSASFNAAMQRLGGRTIPVATEHSSTQKGETLQDTVRTLGCYSDAIVLRHPDDSSAATAAKFSPVPIINGGNGAMEHPTQAFLDLFTIREELGTVNDITITFTGDLKYGRTVHSLVKLLQFYEVRVQLVSPKALAVPEEVRQQLVSSGQLILESTELTPQIIARSDVLYCTRVQKERFEDLGEYERLKNAFVIDNTVLKHAKSQMIVMHPLPRNQEVCEEVDFDQRAAYFRQMKYGLYCRMALLALVMAS

Target ORF information:

RefSeq Version	XM_031720420.1
Organism	Blastomyces gilchristii SLH14081
Definition	Blastomyces gilchristii SLH14081 protein pyrABCN (BDBG_01634), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031720420.1

ORF Insert Sequence:

ATGCCGCTTC TCAATGCCGC TGGGGATGCC AAAAGCTCTC CTGGCCAGTC TCATTCCGTC 
AGGAGCAGAC GACTAACCGA TACTACTGTT ACTCCGTCCA GCCCTGCTTC TGGTGGCGTC 
GTCGCATACA CCACCCAGAA TAAAGACCTG CCTTCCCTCG CTCCCCAAAC GGCATATTCC 
CTCAATGGCG GTGCTATCCC CCCCGCCTCT GCCCCAATGG CTGGCGAAGG GGGAGATGGG 
AGCAAGTGGT ATGCCCTAGA GCTGGAGGAT GGCACCGTCT ACCAGGGTTA CAGTTTTGGT 
GCCGAAGCGA GCATTGCTGG CGAGTTGGTT TTTCAAACTG GCATGGTGGG CTACCCAGAA 
TCAATCACAG ATCCTTCCTA CCGTGGCCAG ATCTTGATCA TCACCTTCCC GCTGGTAGGC 
AACTATGGCG TCCCTTCGCG AGAAACGGTT GATGAGCTCC TCGGGCAAGA TATGCCCAAA 
TATTTCGAGT CCAGTGAGAT CCATGTTGCT GGTCTTGTAG TGGCCAATTA CTGCGGAGAG 
GACTACTCGC ACTTCTTGGC CGAGTCGTCT CTCGGGAAGT GGTTGAAGGA ACAAGGAGTT 
CCTGCCATGT ATGGTGTTGA TACCCGCGCC CTCACAAAGA GAATTCGAGA GAAGGGAAGC 
ATGCTTGGCC GCATGCTTTT GCAGAAACCC GGTGTGAGTG TTGAAACCTT GAATAGCGAC 
GCGCCGAAAA ACGGCTCGAC TACTGCTGTT GTTGATGACG AAGAGAAATC CGATCTTTGG 
AGAGCATCCT TTGAACAAAT TGAATGGATG GACCCTGATA AGAGGAACCT GGTCAACGAT 
GTCTCTATTA GGAAACCCCA GCTGTTCTCC CCACCAAAGG ATTCGGTCCT TTCTCACCCC 
TGTGGTCGCC CGATCAGAGT TGTGTGCGTT GACGTTGGTA TGAAATATAA CCAACTACGG 
TGCCTTCTCG CTCGCGGCGT CGAGCTCCTT GTTGTACCAT GGGATTATGA TTTCCCCAGC 
TTGGCAGGAA AGGAATATGA CGGCCTTTTC CTTTCCAACG GCCCGGGCAA CCCCGCCACC 
CTGTCCAAAA CAGTGGAGAA TATTAGAACC GCCATCAAGG ACTCGCGCAC TCCAATCTTT 
GGTATTTGTC TTGGACACCA GTTACTTGCC CGGGCTGCGG GTGCGTCTAC CATTAAGATG 
AAGTTCGGTA ACCGAGGACA AAATATCCCC TGTACCAGCC TTATCTCCGG TCGCTGTTAC 
ATTACTTCGC AAAACCACGG ATATGCAGTT GATTCTATGA CATTGCCAGA GGGATGGGAG 
GAGCTCTTTG TCAACGCTAA TGATGGAAGC AACGAAGGTA TCAGACACGT TAGCCGCCCA 
TTTTTCAGTG TCCAATTCCA CCCAGAGAGC GCCCCAGGAC CCCAGGACAC AGAGTTCCTA 
TTCGATGTCT TCATCGATGC TATTAAAACG AACGTGGCAT TCCCTAGTGC CTTAGAGAAG 
CCCGTTAGCT TCCCTGGCGC CACCGCTAAA GAGAACGAGC TTGCAAACCC GAGAGTGAGC 
GCTAAGAAAG CCCTTATCCT AGGTAGCGGT GGTCTCAGTA TCGGACAAGC TGGAGAGTTT 
GACTATTCTG GAAGTCAAGC CATAAAGGCT TTGAAAGAAG AAGGAATTTA CACCGTCCTT 
ATTAACCCCA ATATCGCGAC GATTCAGACT TCCAAGGGCC TTGCAGATAA GGTCTATTTC 
TTACCAGTCA ACGCAGACTT TGTCCGCAAG GTTATCAAAC ACGAACGGCC AGATGCCATC 
TATGTAACAT TTGGCGGCCA AACTGCGCTC CAGGTTGGTA TTCAGCTCAA AGATGAGTTC 
GAGGAACTTG GCGTTCGAGT TCTTGGTACT CCGATAGACA CCATCATTAC GACAGAAGAC 
CGCGAGTTAT TCGCGAGAAG CATGGAAGCC CTTGGTGAGA AGTGCGCCAA ATCCGCCTCT 
GCTTCCACGC TCGAGGAAGC CATGGAAGTT GTCAAGGATA TTGGATTTCC CGTCATTGTC 
CGTGCTGCGT ACGCTCTTGG TGGCCTTGGA AGCGGTTTCG CCGAGGATCC TGAGCAGCTG 
CGGGAGCTGT GCACAAAAGC CTTCGCTGCA AGCCCACAGG TTCTTATTGA GAGGAGTATG 
AAAGGGTGGA AGGAGATTGA ATATGAAGTG GTCCGCGATG CTCGTGATAA CTGCATCACT 
GTGTGTAACA TGGAGAATTT TGATCCGCTT GGTATCCACA CTGGCGATTC TATTGTCGTC 
GCCCCGTCGC AAACCCTTTC TGATGAGGAC TACAATATGC TTCGAACGAC TGCTATCAAT 
GTCATCCGCC ATCTGGGTGT GGTGGGTGAA TGTAACATTC AATATGCATT GAACCCATTC 
TCAAAAGAGT ATTGCATTAT TGAGGTCAAT GCTCGCCTGT CAAGATCATC TGCACTCGCA 
TCTAAAGCAA CAGGCTATCC CCTCGCTTTC GTCGCGGCTA AACTTGGCCT CGGAATCCCG 
CTGAATGAGA TTTCAAACTC AGTCACCAAA GTCACATGTG CCTGCTTTGA ACCTTCTCTT 
GATTATGTCG TCGTTAAGAT TCCCCGCTGG GATCTCAAGA AGTTTACCCG TGTCTCCACG 
CAGCTCGGGT CTTCAATGAA GAGTGTTGGG GAGGTTATGA GCATTGGACG GACTTTTGAG 
GAGGCCATTC AGAAAGCCAT ACGTTCTGTC GACTTTCACA ATCTTGGGTT CAACGAGACC 
AACGCACTCA TGTCGATTAA GGCTGAGCTT CAAACACCTT CCGATCAGCG ACTGTTCGCT 
ATCGCCAACG CCATGCATTC GGGCTACACT GTCGACGATA TCTGGGAATT GACCAAAATC 
GACAAGTGGT TCCTCCGAAA ATTGAAAGGC CTCAGCGATT TTGGAAAACT AATGTCTGGT 
TACAACGCCA CTACAGCACC CATTTCTCTC ATTCGACAGG CGAAGCAGCT CGGTTTCTCG 
GACCGGCAAC TGGCGAAGTT TTTGAGCTCC AATGAGTTGG CTATCAGGCG ACTGAGAGTG 
GAAGCAGGCA TTGTGCCCGT GGTCAAACAA ATTGATACCG TCGCTGCTGA ATTCCCGGCA 
TTCACGAACT ACTTGTATCT GACATACAAC GGCTCCGAAA ACGATGTGGA CTTTAACGAC 
CAAGGTGTTA TGGTTCTTGG CTCTGGTGTC TACAGAATTG GATCCTCTGT CGAGTTTGAT 
TGGTGTTCTG TGCGAGCCAT TCGAACTCTT CGAGAGCAGG GTTTTAAGAC GGTGATGGTG 
AATTCCAACC CCGAGACTCT CAGCACAGAT TACGACGAAG CAGACCGACT GTATTTTGAA 
AATATCGATT TGGAGTCCGT CCTTGATGTC TACCAACTTG AGTCTGCAAG CGGGGTTATT 
GTATCTATGG GAGGACAGGC ACCGAGCAAC ATCGCTCTTC CCTTACACAG ACTCCATGTT 
AATGTCCTTG GTACATCTCC GGAGATGATT GATACAGCTG AGAATCGGTA CAAATTTTCG 
CGACTCCTTG ACCATATTGA GGTCGATCAG CCAGCTTGGA AGGAATTGAC CAGTATCGAG 
GAGGCCACCG AATTCTGTGC GAAGGTTGGA TACCCAGTAC TGGTGCGGCC TTCGTACGTG 
CTCTCTGGTG CTGCCATGAA CACGGTTTAT TCTTTGGATG ATCTTGCAAG CTACCTCAAC 
CAGGCTGTTG AGGTCTCCCG TGATCACCCT GTGGTGATTA CCAAATACAT AGAGAACGCC 
AAGGAAATTG AAATGGACGC AGTTGCCAAG AATGGTGTTA TGGTCGGCCA TTTTATTTCA 
GAACATGTTG AGAATGCGGG TGTCCATTCT GGCGATGCTA CTTTGATTCT TCCGCCCCAG 
GACTTGGACC CGGAAACCGT CCGGCGAATC GAGGAAGCGA CCCGCAAGAT CGGCAATGCC 
CTGAACGTCA CTGGACCTTT CAATATCCAG TTCATCGCCA AAGATAACGA CATTAAAGTT 
ATCGAGTGCA ATGTCAGAGC ATCTCGATCC TTCCCGTTCG TTTCCAAAGT CATGGGAGTT 
GACTTGATCG AAATGGCAAC GATGGCAATG ATTGGCAAGC CGTTCACTGA GTACCCGCCG 
GTAACCATTC CCAAGAACTA CGTCGGTGTC AAGGTTCCGC AATTCTCATT CTCCCGCCTC 
TCTGGTGCTG ACCCCGTGCT CGGAGTGGAG ATGGCTTCTA CCGGTGAAGT TGCTTGCTTC 
GGTCGTGACA GGTATGAGGC ATATCTCAAA GCTCTGATTT CGACTGGCTT CAAGCTTCCG 
AAAAAGAACG TCCTGTTCTC CATCGGTTCC TTCAAGGAGA AGGTAGAGAT GTTGCCGTCT 
ATCAGAAAGC TGCACGCGCT TGGCTACAAC CTTTTTGCCA CGGCGGGCAC TGCGGACTAT 
CTCAAAGAGC ACGGACTTCC GGTCAAATAC CTCGAAGTTC TTGCGGGAGA GGAGGAGGAC 
CTTAAGTCTG AATATTCTCT TACGCAGCAT TTGGCCAACA ATCTGATTGA TCTTTACATC 
AATCTTCCCT CCAGCAATAG GTTCCGGCGG CCTGCAAACT ACATGAGCAA GGGCTATCGA 
ACACGGCGAA TGGCAGTCGA CTACCAGACC CCGCTAGTCA CCAACGTTAA GAACGCGAAA 
ATCCTGATCG AAGCGATTGC ACGCCATTAC ACTATGAACG TGATGAGCAT TGATTGCCAA 
ACGAGCTATC GCGCTGTTAC TATCCCCGGC CTAATCAACG TCGCAGCGTT CGTGCCAGGA 
CTCGCTAACC CAGATTCTCC AGATTTCCAG GTGGTGACTA AGGCTTCCAT TGCGAACGGT 
TTTAGCATGG TCCGCATCAT GCCCGTGGGC GTGGATAGCT TCGTTACTGA CTCTCAGGCG 
TTGAGAACGG CCCAACATAA TTCGTCAAAG GGATCGTACT GCGATTTCAA CCTCTCTGTC 
ACGGCAACAT CTAATAATTC AGATAAGCTC GGCCTAGTCA CCAGTGAAGT CGGTTCGTTA 
TTTATCCCAT TCAACCACCT ATCAGGCAAC ATCAACAAGG TGGCAACGGT TACTTCGCAC 
TTTGCATCAT GGCCGACCAA TAAACCTATA ATCACCGATG CCAAAGCTAC GGACCTGGCT 
TCCATTTTGC TGCTTGCCAG TCTTCACAAT CGCAATATCC ACGTCATGAG CGTTACTAGC 
AAAGAGGACA TGGGCCTGAT TGCGCTTAGC AAGGAAAAGG GTCTGAAAGT GACTTGCGAT 
GTTGCAATCT ACTCCCTTTT CCTGTCTACC GATGATTACC CTGGTTGCGC ATGCCTTCCT 
TCGAAGAAAG ACCAAGAAGT GCTCTGGGAG CACTTGGATA CGATTGATAT ATTTTCTATC 
GGTAGTCTTC CATACCAGGT TGCGGGTAGC GATGCTTCTC CCGCTGTTGG GATCCAGGAT 
GCGCTTCCTC TGCTCTTCAC AGCAGTTTCT GACAGGCGAC TAACTATCGA GGATGTTACC 
GCCAGACTCC ACGATAACCC GAAGAGGATC TTTGAACTTC ATGACCAGCC AGATGCCTCC 
GTGGAGATCG AGTTTGACAG GCCATATGTA TTCCAGAGCC ATGATGTCTG GTCACCATTT 
GCCGGTAAGA CTCTAAAGGC ATCCGTCCGA CGAGTTACAT TCCAGGGGAA GACCTCTTGC 
TTGGACGGTG AAATGATGCT GGATGCACCG AAGGGGGCCG ACATGTCCTC TCATCGGTTA 
GCGCCACCGG CTCCAGCATC GCCGGCAGTC AAAGACATTC TTCCCACCTC TCCTCGATCG 
GAAAGTGCCT TTGGGAGACG TTTGTCTTTC TCGGCCACAC CGCTTCAGCG CCCGTCGAGA 
TTCAAACCTG CTGCTCCTGA TGGATATGCT CCATCTGTCT CAGGAGACGA CTTTGTGCCT 
GCCGCTCCGA TTTACCCATA TCCGTCCCGG ATTTCACCCT CATTGCAACA GCTGCTTTCA 
CAATCGTCTT TCAAGCAGAA GCATATCCTA TCTGTCAGCC AGTTCACCCG CCCAGATCTT 
CACCTTCTAT TCACGGTTGC GCAAGAGATG AGGCTGGGTG TCCAACGCCA TGGTGTCCTT 
GACATCCTGA AGGGCCGCGT CCTCACAACC CTCTTCTTCG AGCCCTCCAC TCGTACGTCT 
GCTTCGTTCA ATGCCGCTAT GCAACGACTG GGTGGCCGCA CCATCCCCGT CGCGACTGAG 
CATTCGTCCA CTCAAAAGGG CGAAACCCTT CAGGACACTG TACGCACCCT GGGCTGCTAC 
AGCGACGCGA TCGTCCTCCG TCATCCGGAT GACAGCAGCG CTGCCACCGC CGCCAAATTC 
TCTCCAGTTC CCATCATCAA CGGCGGAAAC GGAGCCATGG AACACCCCAC CCAAGCCTTC 
CTCGACCTCT TCACCATCCG CGAGGAACTC GGCACCGTCA ACGACATCAC AATTACCTTT 
ACCGGCGATT TAAAATACGG CCGCACCGTG CACTCCCTCG TCAAACTCCT CCAATTCTAC 
GAAGTTCGCG TCCAGCTCGT CTCGCCCAAG GCGCTCGCTG TCCCCGAGGA GGTCCGTCAA 
CAGCTCGTCT CGTCCGGCCA GCTGATCCTG GAGTCGACGG AGCTTACTCC GCAGATCATC 
GCACGGTCGG ATGTGCTGTA CTGCACCCGG GTGCAGAAGG AGCGGTTCGA GGACTTGGGC 
GAGTATGAGC GGTTGAAGAA TGCATTCGTC ATCGATAACA CAGTCCTTAA GCATGCGAAG 
AGCCAGATGA TCGTTATGCA TCCTCTGCCG CGCAACCAGG AGGTCTGCGA GGAGGTCGAT 
TTTGACCAGC GCGCGGCTTA TTTCCGACAG ATGAAATATG GGCTCTACTG TCGCATGGCA 
TTACTGGCAT TGGTCATGGC ATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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BDBG_01634 cDNA ORF clone, Blastomyces gilchristii SLH14081

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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