BDBG_03360 cDNA ORF clone, Blastomyces gilchristii SLH14081

Gene
Clones
gRNAs

Summary

Gene Symbol	BDBG_03360
Entrez Gene ID	8505584
Full Name	HEAT repeat protein
Gene Type	protein-coding
Organism	Blastomyces gilchristii SLH14081

mRNA and Protein(s)

mRNA	Protein	Name
XM_002626150.1	XP_002626196.1	HEAT repeat protein
XM_031721215.1	XP_031577687.1	HEAT repeat protein

Pathways from BioSystems
Gene Ontology

The following BDBG_03360 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BDBG_03360 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl04509	XM_002626150.1 Latest version!	Ajellomyces dermatitidis SLH14081 HEAT repeat protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAl115268	XM_031721215.1 Latest version!	Blastomyces gilchristii SLH14081 HEAT repeat protein (BDBG_03360), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BDBG_03360 ( XM_002626150.1 ) cDNA ORF clone, Blastomyces gilchristii SLH14081 -> XP_002626196.1 Ajellomyces dermatitidis SLH14081 HEAT repeat protein, mRNA.

Price & Availability↑

CloneID	OAl04509
Clone ID Related Accession (Same CDS sequence)	XM_002626150.1
Accession Version	XM_002626150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254931200000
Organism	Blastomyces gilchristii SLH14081
Product	HEAT repeat protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101678). COMPLETENESS: incomplete on both ends.

ATGGCGACAG CACATTCTGG GCGCCAGGGC AAGCCTGCCA AGCTCTTAAA GAAGGGCACA 
GCTACGACAA AATCCCACCG ATTCGAGTCT TTCTCCCAGC GCGTCGCCAA ATTAAAAATC 
GATCCTATCC ATCGTGTGCG ACGGGCCAGC GTTGGCGATG ACAGCGACTC TGAGTCCGAT 
TCGTACTTCA GGGCCTCCCT GGAGCACTGG GTGGAACTTA ACCTGTCAGA AAACTTCACT 
CAGTTCTCCC AGCGAGTTAA TCGGCTTTGC GAAAGCTTAC CGCAGATTCT GCATCATGAA 
GATCGAATAA TGGGATTATT GGCCGAGTAT ATCGGGAAAA GAGATGAGTT GAGCATGGAA 
CCGCTGCTGA GTCTTGTAGC TCAGTTTGCA CGAGACTTGG GCCAGCGATT CGAAAGACAC 
TTTGCGTCGA CCGTCACGTT GGTGGCCTCC GTCGCAGCCA CACATCCAAA TATCGAGGTT 
ATAGAATGGA GTTTTACATG TCTGGCATGG ATTTTCAAGT TCCTGTCCCG CCTCCTTGTT 
CCGGACTTAC GCCAACTACT CGGGATAATG TCCCCGTACC TTGGGAAGGA AAAGCAAAAA 
CACTTTGTGA CAAGATTTGC TGCGGAGTCG ATGTCCTTCC TGGTTCGGAA GGCCGCGCTT 
GTCTACTACA AAAATAAGGC GCCACTCGAC AGAGCCGTTA CGTTTCTTTT TGAGGATCTC 
GCCAGCACTG ATGGGGAACG TCAGATTTCA ATGTACCAAC AAGGCCTTAT GTCAATGTTT 
TCTGATGCGA TTAAAGGAGT GAATGGTGGT ATCCATTCGA ATGGAGCTGA CATCATACGT 
TGCCTAATCA GTGCCGCCGC TGTGAAAGAT GAAGCCCAAA GCGCCCTCGC AGAGCAGGTT 
CTTAGTGGGG TTGTAATTAA TTTGGTTCAT CACACATCCT CAAACACCTT TGGTTCGGTG 
CTTGATGTGA TCTGTGATCA CATTGGATCC GCTAAACCGA ATGGTAACAT TGCAGCGACG 
AAGATTGACA TCCGCTTGAT TTTTGTTGCC GTAACCGCGC GCAAGGGTTC TAGAGTTAGC 
AACTGGAAAA GAGTACATCA AACTCTCATG ACCCTTCTTG GTCGCATCGC TAAATCGCCC 
AATATCCCGG CGGATTCAGT TCAACTCCTT TTAGCTTCCG TGGTGACTGC CGTGCATACG 
TCCCCGATGG ACGAATTGCT GTCGTTTATG CGTCCTATTA TGGAGTTAAT TAGCGGCGAC 
CGATTGCGGG ACTACTTCCT CCCGTTTTGC TCGTTATTCT CTACTTTCGG GACCGATCGA 
TTTCAAAGCG TTGTACTCCC ATACTTTCAG CGGCCCGGAT CTTTAAATTG CCCTGGTGGC 
TGGAAGGAGG CTATCGTGGA AAGATTCAGC AAACTCGATC CCACAGCCGA GGATATCGCT 
TTCCTACATT CCTATACCAA ACTCCATGAG GCTATATCAC TCACTTCCGA GCCCTCGATT 
CTTCCACAGC TAACCCACTG CCTCCACGAT CGGTTAAAGA TTGCTATGGC ACTCTCCCCC 
AACGTTGGAG AGGGCCAGGT CACATTTGCC CGAGGCCAAG GGTTTTTGGC TTACGTCCGC 
CTTGCTACGG AACTAGGCTC ACTTGATTTA GGTCTTTGGA GTATGATAGT GTCCAACGGC 
CCTGAGTGCT CAAATTGTCC GGTTTTCCTT GAAGCAACCT TGGCTTATTT GTCTGCTTGT 
ACCAAATACG AGCAGGTCCC GGACGCAGAC CTTGAACCAT TTGCTACAAC TCTGATTAAT 
AACCTTTCTG GCCCATCGCA CGACATCAGA CAATTGTCAC TCAAGATTCT CCAAGTTTTA 
TTGTCCAGGG TGAACGATAG CGACAGTACT TGCATAGAAA TAGCGATCGA AATCGAACAG 
AGCGAGCTTA CTCTGCATGC ATCAAGGGCT CTATCCATGC AGGTACGCAA ACTTGCGATT 
GCATACCCAA CGGTGTGTTC CAAAAAGTGG TTGGATCGAA TTATTCCAAA TTTCTGTTTT 
GGTTTGCTAT CTAAACGGCT CGCGTCGCTG TGGGATGACG CATGTGAGGC AATCAAGGCA 
ATTTGCGAGG TTCCAAATGG AGAAAGCATT GTTACGGAGC TCGCCATGCG CGGGCTACAA 
GAGCCTAGTC CGCTTGGAAC CAATGCAAAT ACCCCCGAAG AAGAATCCGC TGAATCGCAT 
ATTTCGTCGG AATTCGAATG CTTTAACGTT TCAAATGTCG AGAAAACTAT GTGTGCGACT 
TTCGCTGACT CCCGCAACTC TAATTCGTTG CTCGCAGAAG ATTTCAAGCG TGCTCATGTT 
ACGGAACCTC AACGTTCTAC TTCTAGTCGA ACACAGGCCC TCAAGGTCCT GGGATCTGTA 
CCTCAAGTGG CGGAGAAACG ATCTCGGCAA TTTGTTCCTC TATTCCTGAT ATGGGCAGCT 
CATGATGATG ACATCCCTAC CGCCAACGAA TCCAAGCCTT CTGGTACAAG TGGCACAGAT 
GATAGTTCTG TTTCTTCCTG GAATCTGCGT GACCGGAAAG CTATGCTGGA CATCTTCGGA 
AAATTCGTCA ACCCCAAAGT TTTATACAAA GCAGCAGAGG TCCATGAAGC TTTGCTTGGC 
CTTCTCGGTA ATGGAGATAC TGAAATTCAA AAATCTGCAC TGAAGGCTAT TTTCACATGG 
AAGTCACCTG CAATTCAACC TTACGAGACG AACATTTTGA ATATTGTGGA TGATGCGAGA 
TTCCGAGATG AACTATCAGT GTTCTTAAAT GCTGGCCAGG AGGCTAGTAC GATTAAGGAA 
AATCATCGTG CTGAGCTATT CCCTGTTCTG CTGAGACTTC TATATGGCAA GATTGTCGCC 
CGTGGGGGCT CTAGGGGTGG ACAGGGGACC CAAGAGGCAC GCAGAAAGAC CATTTTAAGA 
ACAATATCCC AACTCTCTGA AAGCGACTTT GGTCACTTCG TTCAAATAGC GTTTGGTCCC 
CTGAGCGATA TTCGCGTCCT CGAAACCGAC CCTGGACATG GCCAGTGCCT CAATCAGGAG 
GTGATGGGTG TTAGAAAGCA ATATGGCCTT CTGAAGATGG TGGAAACTAT GTTCGAAACC 
CTGAAAACCA AGATGTCACC TTTCGTTGAC CGCTCCATGA ACGTAGTTCT ATACTGTCTT 
GTCAGAGCTT GCAGGCAGTT GAACGATGCC AGTTCAGAAG AGAATCATTC GTCTCTCATC 
CGCAATACTC GGCAGACAGG TGTCCATTGT CTCGATCTGA TATTTTCTGT TTCGCCCGAT 
TATGATTGGA CTCCATATAT ACCCCTGATT TTAAACGAGA TCATCAGCCC AAGATTAGGA 
AATTTCGCCG TGGAAACAGC GCAAAGTGTG TCCGGCCTTC TTCATCTGTT CCACACCTGG 
GCGTCCAGCC CCAAGTCAGC TCTCTACTTC CTGCATGCTG ACCAACAGGT CATTCAACGA 
ATTGTTGACT GCCTTGGCGT TGAATCTGCT CGCGAAGACG TCAAGATATT TGTTCTGAAT 
GAAATTCTTA ACAACCTCGT CAGTCGCGCG AATGGCGAGA CAGACGACCC AGAGAATTTG 
CCAGATGCCG CAACAACAGA TCTAATCCGG TCTAAAGTGC TGGCACCCAA TGTTGAATAC 
ATATTGACAC ATGTCGAATC CCTGCTCAGG AATCAATCTA CGCGGCAACT TACGATGGCT 
GCAATTGAGA CCCTATCTAA ACTCGCGCCG TTTGTCCAGT CATCGCAAGA AACCACGAAA 
CTAATTAGTA CGGCTACATT TCTCCTTAAC GAACCTGCAG ATCGAGTACC CCCCAAAGCC 
AAAAGCGGAC TTCTGCGTGT TATGCATCAT TTCCTCCCCC TATATGATCC ACGCCAAAAT 
GTGGACCTCA ACCAGCGAAT CTTTGAAGTA ATTTCCTCCC TATTCGATTA CTTCAAAGAT 
AACCCGAACC GTGCTATGCT CTCATCCGTG TTGTCTGCAT TTGCAAACCA CGAAGTGGAC 
CTAAAAGGTG TTGCCGAATT ATGTGCAGAC CTCAACGCTA TATCAGAGAA AAATCTTGAT 
GAATTAGATT TCGACCGACG GCTTAGGGCA TTTAACACAA TAAACGAACA GAAGTACAAG 
GAGTTCACAG CTAGGCAATG GCGCCCGCTG TTATTCAATC TTCTATACCA TGTTAAAGAC 
GAGGAGGAAC TTGCAATTCG ATCAAGTTCA TCATTTGGAC TGAAAAGGTT TATCGAGAGC 
GTGGGTTCAA AACCAGAGGG TGATAGCGAA GACTACTCAT TTTTGCTCGA GAAAGCTTTG 
TTGCCTGCGT TGAGGAACGG AGCAAAACAC CCTGCCGAGA CAGTTCGGGC CGAGTTCGTC 
TCTGTCTTGG GTTATTTCAT AACGCATAAC CCGAACCATC GGGCCGTGAC CGACATGGTG 
GACTTACTAG CAGGTGGGGA TGAAGAGGCA TCGTTTTTCA ATAATATTCT CCATATTCAA 
CAACATCGTC GTCTTCGTGC CTTGCGGCGA CTGGCTACTG AGGCTAGACA AGGAAAGTTT 
GAGTCATCAA ATATTAGCTC CTTTTTCTTA CCTCTTATTG AACATTATGT GTTTGATCAG 
GCAGAAGACG ATAGTGCCCA TAACCTGGCC GCGGAGACAG TTACAGCTAT TGGGGTGCTG 
TCTGAAGGGC TGGAGTGGAG CCAGTTTAGA GCGATATTCC GCCGATACAA GGGATATGTA 
CGAAGTCGCC CTGGGATGGA AAAGAACGTT ATACGTCTTC TAGGACAGAT GACCGATGGC 
TTGTCTCGAG CAATGAGTAT TGATAAAGAA GAACAGCCGG TTGCTGGGGA TGGAGAAATG 
GAAGGAATTG AGAAAGAGTC TACGATTAAA AGCACGCTCT CGAAGACTTT GCCTGCGGAG 
GCTCGCATCG GGACAGAACT GAAAACCAAC TTCATCCCTT TCTTATCCGA GTTTGTACAC 
CAGAAGGATG AATCCGAAGT TCATCTCCGT GTCCCAGTCG CAGTCATAAC TGTTAAGTTA 
CTCAAACTTC TTCCTAAGGA TGAAATGGCA CTTCTTCTCC CATCGGTACT GCTAGACCTA 
TCGAACGTTC TTAGAAGTCG GTCTCAGGAT GCTAGGGATA TGGCCAGAAA AACACTCGCT 
GATGTCGCGC TTATTCTTGG ACCTTCTTAC TTTGGATATA TTCTGAGAGA ATTGCGGAGC 
ACGTTAACAA GAGGATACCA ACTCCATGTG CTGTCCTTTA CAGTCCATTC GATTCTTGTA 
GCCACCAGCG ACCATTTCGA GCCTGGTGAC CTCGATCACA GTCTCGACGC ACTAGCAGCC 
GTGGTGATGG ATGATATTTT CGGCACCGTC GGCCAAGAGA AGGACGCCGA GGAGTACGTG 
AGCAAAATGA AGGAGGTAAA GAGTAACAAA AGCTATGATT CAATGGAGCT ACTCGCGAAA 
ATATCGACAA TCCGCCATCT CTCGGCTCTT ATTAACCCAG TGCAAGCGCT TTTGCGAGAG 
AAACTTACGG CTACCATCAT CAGAAAAGTC GACGAACTGC TTAGACGGAT CGGTGTCGGC 
CTGCTGCGGA ATCCTGGAGC TGAAAGCCGT GATCTTTTGG TTTTCTGCTA CGAGGTGATA 
AAGGAGTCAT ACAAAGCAGA CACACCACAA ATTGAAACAC AGGAGCAAGG TAACAGGGCC 
CGTTTCATCG TCAATTTGCT TAACCCGAGG AACCGTATGA ATCGAGGAAG CACATCTTCC 
TATTCCTACA AGCTTTCTCG CTTCGGACTT GATGTTCTCC GCTCCGTGCT AAATAAGTAC 
CAATCTCTCA TGACAGCCGC CAACGTTGCA GGGTTCCTTC CCATTATTGG AGATGCATTG 
CTACAGAGCT ATGAGGAGGT CAAACTGTCT GCCATTAGAC TCATATCCAC GATCATCAAA 
GTCCCACTTT CCGAGCTCGA TAGAAACTCT GATGTATATC TAATGGAAGC TATTAAGTTG 
GTCAAAGAAG CTCCGAACAC AAATACCGAA GCTGCCCAAG CTGCCTTGAA GCTCATCGCT 
TCCATCCTAC GCGAGCGTAA GAGTACAAAG CTCAAGGACT CCCACCTGGG CTATTTGTTG 
AAACGAATAG CTGCTGACAT CGAGGAGCCT GACCGTCAAG GAGTGGCTTT CAATTTCATT 
CGGGCAGTGA TGTCTCGCAA ATTTATTGTT CCCGAGATGT ACGAGCTTGT CGATAATGTT 
GCTGCGATGA TGGTAACAAA CCACACAAGA AGTGCTCGCG ATCTCGCAAG AGGTGTCTTT 
ATACACTTTA TGATTGAATA CCCTCAAGCT AAAAGCAGGT GGACGAAGCA ACTTGGATTC 
CTCGCCAAAA ACCTTGACTA TCAACATAAA GAAGGCCGTC AGTCCGTTAT GGAAGCAGTT 
CATCTTCTTC TCTCCAAGAC CAATGGCGAC CCTGCTCAGG ATATTGTGGG TAACTTTTTT 
GTGCCAGTGG TAATGGTAAT GTCTAATGAT GAGGCAGTTG AATGTCGAGA GATGGCTGGT 
GTTCTTCTTA ATGATCTCTT CAGCCGTGCT GATTCAGAGC AGCTAAAGTC TATGCTCTCT 
CCTCTTCGCT CCTGGTTGGA GCAATCCCAA AACACACTGC TCACTGCCAC TGGGTTACAA 
GCGATCAGGA TATTCTTTGA GTCGCATAGG AACAATAAAG AAGGCGAAAT GCGTTTTGTT 
ACGGATTTAT TGCCTCGAAT CCTTGGCCCC GCAGTGGAGA ACTGCGAAGG GGAAGAGTGG 
GAAGTCCTCT ACCATTCGCT ACAGCTCTTT ACAAAACTAT GCCAGCTCTA TCCAGCGGTT 
GCCATGTCCG AGGATTGCGA GAACATCTGG CGCTATGTTT GCGAAAGCCT CTTCTATCCA 
CACGCGTGGA TCAAGTCATG CGCAGCAAAT TTAATTGGAA TATGGCTTGC AGACGAAGCG 
AAAGGAAATG CCGTTACTGG TTATGGCTCT GTCCCACTCA TCGGCGCATC TGGTCTTAAG 
CTCGAAGGCT GCATGATGTT AGATATTACG CGAGCCAGTC TGCTATGTCT GAAATCAACC 
ACTATTTCCG AAGATCTCGC AACTCAGACA ATCCGGAATC TGGTGTTCTT AGGACGCTGC 
TTCAGCCAGA ATAACCTTGC GTTTTCGAAG AAGGCTGAGG AGCTGGCTGG TGATTTCAGC 
GACGACAGCG ACGATGAAGA CAACATTGAC CCAGCTACGG TGAATTCCGC AAAGCAAAAC 
AAGCCAGCAA TTGAATATAT TTTCGAACGA GCTGCTGCCA TCCTTCGACG AGAGCCTCGA 
ACCACCCGTG CAGACTCTTT AATCAATAAA ACGGCTTGCA TGAAACTTCT CGCAGCTCTC 
TGCACTCATC TTGACGTCGC TCAAATTACT CCGTCCCTCC AGACAATCCT TCTTCCGTTA 
ATACATCTAA CCGACCCATC GATCCCTGCG CCTCGCTCCC TCGATGAGCA GTTCCAGGCT 
ACTTACAAAG CCCTTGTTAG CAGCAGCCAG GAGATCCTCG ATTTGTTACA GAAGAAGTTA 
GGTACGACTG ATTTCGTGAC GCAAATGGCG GCGGTTCGTG AGAGTGTCAA GTCAAGACGG 
GAGGGTAGAA GAGTTAAGAG GCGCATCGAA GCTGTTGCTG ATCCTGAGAA GTTCGGCAGA 
GAAAAGAAGA GGAAGAATGA CCGGAAGCGA GAGAAGAGGA AGGAAAAGGG CGCAGAATAT 
CAAGATCGGA GACGTGGCTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002626196.1
CDS	1..7764
Translation	MATAHSGRQGKPAKLLKKGTATTKSHRFESFSQRVAKLKIDPIHRVRRASVGDDSDSESDSYFRASLEHWVELNLSENFTQFSQRVNRLCESLPQILHHEDRIMGLLAEYIGKRDELSMEPLLSLVAQFARDLGQRFERHFASTVTLVASVAATHPNIEVIEWSFTCLAWIFKFLSRLLVPDLRQLLGIMSPYLGKEKQKHFVTRFAAESMSFLVRKAALVYYKNKAPLDRAVTFLFEDLASTDGERQISMYQQGLMSMFSDAIKGVNGGIHSNGADIIRCLISAAAVKDEAQSALAEQVLSGVVINLVHHTSSNTFGSVLDVICDHIGSAKPNGNIAATKIDIRLIFVAVTARKGSRVSNWKRVHQTLMTLLGRIAKSPNIPADSVQLLLASVVTAVHTSPMDELLSFMRPIMELISGDRLRDYFLPFCSLFSTFGTDRFQSVVLPYFQRPGSLNCPGGWKEAIVERFSKLDPTAEDIAFLHSYTKLHEAISLTSEPSILPQLTHCLHDRLKIAMALSPNVGEGQVTFARGQGFLAYVRLATELGSLDLGLWSMIVSNGPECSNCPVFLEATLAYLSACTKYEQVPDADLEPFATTLINNLSGPSHDIRQLSLKILQVLLSRVNDSDSTCIEIAIEIEQSELTLHASRALSMQVRKLAIAYPTVCSKKWLDRIIPNFCFGLLSKRLASLWDDACEAIKAICEVPNGESIVTELAMRGLQEPSPLGTNANTPEEESAESHISSEFECFNVSNVEKTMCATFADSRNSNSLLAEDFKRAHVTEPQRSTSSRTQALKVLGSVPQVAEKRSRQFVPLFLIWAAHDDDIPTANESKPSGTSGTDDSSVSSWNLRDRKAMLDIFGKFVNPKVLYKAAEVHEALLGLLGNGDTEIQKSALKAIFTWKSPAIQPYETNILNIVDDARFRDELSVFLNAGQEASTIKENHRAELFPVLLRLLYGKIVARGGSRGGQGTQEARRKTILRTISQLSESDFGHFVQIAFGPLSDIRVLETDPGHGQCLNQEVMGVRKQYGLLKMVETMFETLKTKMSPFVDRSMNVVLYCLVRACRQLNDASSEENHSSLIRNTRQTGVHCLDLIFSVSPDYDWTPYIPLILNEIISPRLGNFAVETAQSVSGLLHLFHTWASSPKSALYFLHADQQVIQRIVDCLGVESAREDVKIFVLNEILNNLVSRANGETDDPENLPDAATTDLIRSKVLAPNVEYILTHVESLLRNQSTRQLTMAAIETLSKLAPFVQSSQETTKLISTATFLLNEPADRVPPKAKSGLLRVMHHFLPLYDPRQNVDLNQRIFEVISSLFDYFKDNPNRAMLSSVLSAFANHEVDLKGVAELCADLNAISEKNLDELDFDRRLRAFNTINEQKYKEFTARQWRPLLFNLLYHVKDEEELAIRSSSSFGLKRFIESVGSKPEGDSEDYSFLLEKALLPALRNGAKHPAETVRAEFVSVLGYFITHNPNHRAVTDMVDLLAGGDEEASFFNNILHIQQHRRLRALRRLATEARQGKFESSNISSFFLPLIEHYVFDQAEDDSAHNLAAETVTAIGVLSEGLEWSQFRAIFRRYKGYVRSRPGMEKNVIRLLGQMTDGLSRAMSIDKEEQPVAGDGEMEGIEKESTIKSTLSKTLPAEARIGTELKTNFIPFLSEFVHQKDESEVHLRVPVAVITVKLLKLLPKDEMALLLPSVLLDLSNVLRSRSQDARDMARKTLADVALILGPSYFGYILRELRSTLTRGYQLHVLSFTVHSILVATSDHFEPGDLDHSLDALAAVVMDDIFGTVGQEKDAEEYVSKMKEVKSNKSYDSMELLAKISTIRHLSALINPVQALLREKLTATIIRKVDELLRRIGVGLLRNPGAESRDLLVFCYEVIKESYKADTPQIETQEQGNRARFIVNLLNPRNRMNRGSTSSYSYKLSRFGLDVLRSVLNKYQSLMTAANVAGFLPIIGDALLQSYEEVKLSAIRLISTIIKVPLSELDRNSDVYLMEAIKLVKEAPNTNTEAAQAALKLIASILRERKSTKLKDSHLGYLLKRIAADIEEPDRQGVAFNFIRAVMSRKFIVPEMYELVDNVAAMMVTNHTRSARDLARGVFIHFMIEYPQAKSRWTKQLGFLAKNLDYQHKEGRQSVMEAVHLLLSKTNGDPAQDIVGNFFVPVVMVMSNDEAVECREMAGVLLNDLFSRADSEQLKSMLSPLRSWLEQSQNTLLTATGLQAIRIFFESHRNNKEGEMRFVTDLLPRILGPAVENCEGEEWEVLYHSLQLFTKLCQLYPAVAMSEDCENIWRYVCESLFYPHAWIKSCAANLIGIWLADEAKGNAVTGYGSVPLIGASGLKLEGCMMLDITRASLLCLKSTTISEDLATQTIRNLVFLGRCFSQNNLAFSKKAEELAGDFSDDSDDEDNIDPATVNSAKQNKPAIEYIFERAAAILRREPRTTRADSLINKTACMKLLAALCTHLDVAQITPSLQTILLPLIHLTDPSIPAPRSLDEQFQATYKALVSSSQEILDLLQKKLGTTDFVTQMAAVRESVKSRREGRRVKRRIEAVADPEKFGREKKRKNDRKREKRKEKGAEYQDRRRGW

Target ORF information:

RefSeq Version	XM_002626150.1
Organism	Blastomyces gilchristii SLH14081
Definition	Ajellomyces dermatitidis SLH14081 HEAT repeat protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002626150.1

ORF Insert Sequence:

ATGGCGACAG CACATTCTGG GCGCCAGGGC AAGCCTGCCA AGCTCTTAAA GAAGGGCACA 
GCTACGACAA AATCCCACCG ATTCGAGTCT TTCTCCCAGC GCGTCGCCAA ATTAAAAATC 
GATCCTATCC ATCGTGTGCG ACGGGCCAGC GTTGGCGATG ACAGCGACTC TGAGTCCGAT 
TCGTACTTCA GGGCCTCCCT GGAGCACTGG GTGGAACTTA ACCTGTCAGA AAACTTCACT 
CAGTTCTCCC AGCGAGTTAA TCGGCTTTGC GAAAGCTTAC CGCAGATTCT GCATCATGAA 
GATCGAATAA TGGGATTATT GGCCGAGTAT ATCGGGAAAA GAGATGAGTT GAGCATGGAA 
CCGCTGCTGA GTCTTGTAGC TCAGTTTGCA CGAGACTTGG GCCAGCGATT CGAAAGACAC 
TTTGCGTCGA CCGTCACGTT GGTGGCCTCC GTCGCAGCCA CACATCCAAA TATCGAGGTT 
ATAGAATGGA GTTTTACATG TCTGGCATGG ATTTTCAAGT TCCTGTCCCG CCTCCTTGTT 
CCGGACTTAC GCCAACTACT CGGGATAATG TCCCCGTACC TTGGGAAGGA AAAGCAAAAA 
CACTTTGTGA CAAGATTTGC TGCGGAGTCG ATGTCCTTCC TGGTTCGGAA GGCCGCGCTT 
GTCTACTACA AAAATAAGGC GCCACTCGAC AGAGCCGTTA CGTTTCTTTT TGAGGATCTC 
GCCAGCACTG ATGGGGAACG TCAGATTTCA ATGTACCAAC AAGGCCTTAT GTCAATGTTT 
TCTGATGCGA TTAAAGGAGT GAATGGTGGT ATCCATTCGA ATGGAGCTGA CATCATACGT 
TGCCTAATCA GTGCCGCCGC TGTGAAAGAT GAAGCCCAAA GCGCCCTCGC AGAGCAGGTT 
CTTAGTGGGG TTGTAATTAA TTTGGTTCAT CACACATCCT CAAACACCTT TGGTTCGGTG 
CTTGATGTGA TCTGTGATCA CATTGGATCC GCTAAACCGA ATGGTAACAT TGCAGCGACG 
AAGATTGACA TCCGCTTGAT TTTTGTTGCC GTAACCGCGC GCAAGGGTTC TAGAGTTAGC 
AACTGGAAAA GAGTACATCA AACTCTCATG ACCCTTCTTG GTCGCATCGC TAAATCGCCC 
AATATCCCGG CGGATTCAGT TCAACTCCTT TTAGCTTCCG TGGTGACTGC CGTGCATACG 
TCCCCGATGG ACGAATTGCT GTCGTTTATG CGTCCTATTA TGGAGTTAAT TAGCGGCGAC 
CGATTGCGGG ACTACTTCCT CCCGTTTTGC TCGTTATTCT CTACTTTCGG GACCGATCGA 
TTTCAAAGCG TTGTACTCCC ATACTTTCAG CGGCCCGGAT CTTTAAATTG CCCTGGTGGC 
TGGAAGGAGG CTATCGTGGA AAGATTCAGC AAACTCGATC CCACAGCCGA GGATATCGCT 
TTCCTACATT CCTATACCAA ACTCCATGAG GCTATATCAC TCACTTCCGA GCCCTCGATT 
CTTCCACAGC TAACCCACTG CCTCCACGAT CGGTTAAAGA TTGCTATGGC ACTCTCCCCC 
AACGTTGGAG AGGGCCAGGT CACATTTGCC CGAGGCCAAG GGTTTTTGGC TTACGTCCGC 
CTTGCTACGG AACTAGGCTC ACTTGATTTA GGTCTTTGGA GTATGATAGT GTCCAACGGC 
CCTGAGTGCT CAAATTGTCC GGTTTTCCTT GAAGCAACCT TGGCTTATTT GTCTGCTTGT 
ACCAAATACG AGCAGGTCCC GGACGCAGAC CTTGAACCAT TTGCTACAAC TCTGATTAAT 
AACCTTTCTG GCCCATCGCA CGACATCAGA CAATTGTCAC TCAAGATTCT CCAAGTTTTA 
TTGTCCAGGG TGAACGATAG CGACAGTACT TGCATAGAAA TAGCGATCGA AATCGAACAG 
AGCGAGCTTA CTCTGCATGC ATCAAGGGCT CTATCCATGC AGGTACGCAA ACTTGCGATT 
GCATACCCAA CGGTGTGTTC CAAAAAGTGG TTGGATCGAA TTATTCCAAA TTTCTGTTTT 
GGTTTGCTAT CTAAACGGCT CGCGTCGCTG TGGGATGACG CATGTGAGGC AATCAAGGCA 
ATTTGCGAGG TTCCAAATGG AGAAAGCATT GTTACGGAGC TCGCCATGCG CGGGCTACAA 
GAGCCTAGTC CGCTTGGAAC CAATGCAAAT ACCCCCGAAG AAGAATCCGC TGAATCGCAT 
ATTTCGTCGG AATTCGAATG CTTTAACGTT TCAAATGTCG AGAAAACTAT GTGTGCGACT 
TTCGCTGACT CCCGCAACTC TAATTCGTTG CTCGCAGAAG ATTTCAAGCG TGCTCATGTT 
ACGGAACCTC AACGTTCTAC TTCTAGTCGA ACACAGGCCC TCAAGGTCCT GGGATCTGTA 
CCTCAAGTGG CGGAGAAACG ATCTCGGCAA TTTGTTCCTC TATTCCTGAT ATGGGCAGCT 
CATGATGATG ACATCCCTAC CGCCAACGAA TCCAAGCCTT CTGGTACAAG TGGCACAGAT 
GATAGTTCTG TTTCTTCCTG GAATCTGCGT GACCGGAAAG CTATGCTGGA CATCTTCGGA 
AAATTCGTCA ACCCCAAAGT TTTATACAAA GCAGCAGAGG TCCATGAAGC TTTGCTTGGC 
CTTCTCGGTA ATGGAGATAC TGAAATTCAA AAATCTGCAC TGAAGGCTAT TTTCACATGG 
AAGTCACCTG CAATTCAACC TTACGAGACG AACATTTTGA ATATTGTGGA TGATGCGAGA 
TTCCGAGATG AACTATCAGT GTTCTTAAAT GCTGGCCAGG AGGCTAGTAC GATTAAGGAA 
AATCATCGTG CTGAGCTATT CCCTGTTCTG CTGAGACTTC TATATGGCAA GATTGTCGCC 
CGTGGGGGCT CTAGGGGTGG ACAGGGGACC CAAGAGGCAC GCAGAAAGAC CATTTTAAGA 
ACAATATCCC AACTCTCTGA AAGCGACTTT GGTCACTTCG TTCAAATAGC GTTTGGTCCC 
CTGAGCGATA TTCGCGTCCT CGAAACCGAC CCTGGACATG GCCAGTGCCT CAATCAGGAG 
GTGATGGGTG TTAGAAAGCA ATATGGCCTT CTGAAGATGG TGGAAACTAT GTTCGAAACC 
CTGAAAACCA AGATGTCACC TTTCGTTGAC CGCTCCATGA ACGTAGTTCT ATACTGTCTT 
GTCAGAGCTT GCAGGCAGTT GAACGATGCC AGTTCAGAAG AGAATCATTC GTCTCTCATC 
CGCAATACTC GGCAGACAGG TGTCCATTGT CTCGATCTGA TATTTTCTGT TTCGCCCGAT 
TATGATTGGA CTCCATATAT ACCCCTGATT TTAAACGAGA TCATCAGCCC AAGATTAGGA 
AATTTCGCCG TGGAAACAGC GCAAAGTGTG TCCGGCCTTC TTCATCTGTT CCACACCTGG 
GCGTCCAGCC CCAAGTCAGC TCTCTACTTC CTGCATGCTG ACCAACAGGT CATTCAACGA 
ATTGTTGACT GCCTTGGCGT TGAATCTGCT CGCGAAGACG TCAAGATATT TGTTCTGAAT 
GAAATTCTTA ACAACCTCGT CAGTCGCGCG AATGGCGAGA CAGACGACCC AGAGAATTTG 
CCAGATGCCG CAACAACAGA TCTAATCCGG TCTAAAGTGC TGGCACCCAA TGTTGAATAC 
ATATTGACAC ATGTCGAATC CCTGCTCAGG AATCAATCTA CGCGGCAACT TACGATGGCT 
GCAATTGAGA CCCTATCTAA ACTCGCGCCG TTTGTCCAGT CATCGCAAGA AACCACGAAA 
CTAATTAGTA CGGCTACATT TCTCCTTAAC GAACCTGCAG ATCGAGTACC CCCCAAAGCC 
AAAAGCGGAC TTCTGCGTGT TATGCATCAT TTCCTCCCCC TATATGATCC ACGCCAAAAT 
GTGGACCTCA ACCAGCGAAT CTTTGAAGTA ATTTCCTCCC TATTCGATTA CTTCAAAGAT 
AACCCGAACC GTGCTATGCT CTCATCCGTG TTGTCTGCAT TTGCAAACCA CGAAGTGGAC 
CTAAAAGGTG TTGCCGAATT ATGTGCAGAC CTCAACGCTA TATCAGAGAA AAATCTTGAT 
GAATTAGATT TCGACCGACG GCTTAGGGCA TTTAACACAA TAAACGAACA GAAGTACAAG 
GAGTTCACAG CTAGGCAATG GCGCCCGCTG TTATTCAATC TTCTATACCA TGTTAAAGAC 
GAGGAGGAAC TTGCAATTCG ATCAAGTTCA TCATTTGGAC TGAAAAGGTT TATCGAGAGC 
GTGGGTTCAA AACCAGAGGG TGATAGCGAA GACTACTCAT TTTTGCTCGA GAAAGCTTTG 
TTGCCTGCGT TGAGGAACGG AGCAAAACAC CCTGCCGAGA CAGTTCGGGC CGAGTTCGTC 
TCTGTCTTGG GTTATTTCAT AACGCATAAC CCGAACCATC GGGCCGTGAC CGACATGGTG 
GACTTACTAG CAGGTGGGGA TGAAGAGGCA TCGTTTTTCA ATAATATTCT CCATATTCAA 
CAACATCGTC GTCTTCGTGC CTTGCGGCGA CTGGCTACTG AGGCTAGACA AGGAAAGTTT 
GAGTCATCAA ATATTAGCTC CTTTTTCTTA CCTCTTATTG AACATTATGT GTTTGATCAG 
GCAGAAGACG ATAGTGCCCA TAACCTGGCC GCGGAGACAG TTACAGCTAT TGGGGTGCTG 
TCTGAAGGGC TGGAGTGGAG CCAGTTTAGA GCGATATTCC GCCGATACAA GGGATATGTA 
CGAAGTCGCC CTGGGATGGA AAAGAACGTT ATACGTCTTC TAGGACAGAT GACCGATGGC 
TTGTCTCGAG CAATGAGTAT TGATAAAGAA GAACAGCCGG TTGCTGGGGA TGGAGAAATG 
GAAGGAATTG AGAAAGAGTC TACGATTAAA AGCACGCTCT CGAAGACTTT GCCTGCGGAG 
GCTCGCATCG GGACAGAACT GAAAACCAAC TTCATCCCTT TCTTATCCGA GTTTGTACAC 
CAGAAGGATG AATCCGAAGT TCATCTCCGT GTCCCAGTCG CAGTCATAAC TGTTAAGTTA 
CTCAAACTTC TTCCTAAGGA TGAAATGGCA CTTCTTCTCC CATCGGTACT GCTAGACCTA 
TCGAACGTTC TTAGAAGTCG GTCTCAGGAT GCTAGGGATA TGGCCAGAAA AACACTCGCT 
GATGTCGCGC TTATTCTTGG ACCTTCTTAC TTTGGATATA TTCTGAGAGA ATTGCGGAGC 
ACGTTAACAA GAGGATACCA ACTCCATGTG CTGTCCTTTA CAGTCCATTC GATTCTTGTA 
GCCACCAGCG ACCATTTCGA GCCTGGTGAC CTCGATCACA GTCTCGACGC ACTAGCAGCC 
GTGGTGATGG ATGATATTTT CGGCACCGTC GGCCAAGAGA AGGACGCCGA GGAGTACGTG 
AGCAAAATGA AGGAGGTAAA GAGTAACAAA AGCTATGATT CAATGGAGCT ACTCGCGAAA 
ATATCGACAA TCCGCCATCT CTCGGCTCTT ATTAACCCAG TGCAAGCGCT TTTGCGAGAG 
AAACTTACGG CTACCATCAT CAGAAAAGTC GACGAACTGC TTAGACGGAT CGGTGTCGGC 
CTGCTGCGGA ATCCTGGAGC TGAAAGCCGT GATCTTTTGG TTTTCTGCTA CGAGGTGATA 
AAGGAGTCAT ACAAAGCAGA CACACCACAA ATTGAAACAC AGGAGCAAGG TAACAGGGCC 
CGTTTCATCG TCAATTTGCT TAACCCGAGG AACCGTATGA ATCGAGGAAG CACATCTTCC 
TATTCCTACA AGCTTTCTCG CTTCGGACTT GATGTTCTCC GCTCCGTGCT AAATAAGTAC 
CAATCTCTCA TGACAGCCGC CAACGTTGCA GGGTTCCTTC CCATTATTGG AGATGCATTG 
CTACAGAGCT ATGAGGAGGT CAAACTGTCT GCCATTAGAC TCATATCCAC GATCATCAAA 
GTCCCACTTT CCGAGCTCGA TAGAAACTCT GATGTATATC TAATGGAAGC TATTAAGTTG 
GTCAAAGAAG CTCCGAACAC AAATACCGAA GCTGCCCAAG CTGCCTTGAA GCTCATCGCT 
TCCATCCTAC GCGAGCGTAA GAGTACAAAG CTCAAGGACT CCCACCTGGG CTATTTGTTG 
AAACGAATAG CTGCTGACAT CGAGGAGCCT GACCGTCAAG GAGTGGCTTT CAATTTCATT 
CGGGCAGTGA TGTCTCGCAA ATTTATTGTT CCCGAGATGT ACGAGCTTGT CGATAATGTT 
GCTGCGATGA TGGTAACAAA CCACACAAGA AGTGCTCGCG ATCTCGCAAG AGGTGTCTTT 
ATACACTTTA TGATTGAATA CCCTCAAGCT AAAAGCAGGT GGACGAAGCA ACTTGGATTC 
CTCGCCAAAA ACCTTGACTA TCAACATAAA GAAGGCCGTC AGTCCGTTAT GGAAGCAGTT 
CATCTTCTTC TCTCCAAGAC CAATGGCGAC CCTGCTCAGG ATATTGTGGG TAACTTTTTT 
GTGCCAGTGG TAATGGTAAT GTCTAATGAT GAGGCAGTTG AATGTCGAGA GATGGCTGGT 
GTTCTTCTTA ATGATCTCTT CAGCCGTGCT GATTCAGAGC AGCTAAAGTC TATGCTCTCT 
CCTCTTCGCT CCTGGTTGGA GCAATCCCAA AACACACTGC TCACTGCCAC TGGGTTACAA 
GCGATCAGGA TATTCTTTGA GTCGCATAGG AACAATAAAG AAGGCGAAAT GCGTTTTGTT 
ACGGATTTAT TGCCTCGAAT CCTTGGCCCC GCAGTGGAGA ACTGCGAAGG GGAAGAGTGG 
GAAGTCCTCT ACCATTCGCT ACAGCTCTTT ACAAAACTAT GCCAGCTCTA TCCAGCGGTT 
GCCATGTCCG AGGATTGCGA GAACATCTGG CGCTATGTTT GCGAAAGCCT CTTCTATCCA 
CACGCGTGGA TCAAGTCATG CGCAGCAAAT TTAATTGGAA TATGGCTTGC AGACGAAGCG 
AAAGGAAATG CCGTTACTGG TTATGGCTCT GTCCCACTCA TCGGCGCATC TGGTCTTAAG 
CTCGAAGGCT GCATGATGTT AGATATTACG CGAGCCAGTC TGCTATGTCT GAAATCAACC 
ACTATTTCCG AAGATCTCGC AACTCAGACA ATCCGGAATC TGGTGTTCTT AGGACGCTGC 
TTCAGCCAGA ATAACCTTGC GTTTTCGAAG AAGGCTGAGG AGCTGGCTGG TGATTTCAGC 
GACGACAGCG ACGATGAAGA CAACATTGAC CCAGCTACGG TGAATTCCGC AAAGCAAAAC 
AAGCCAGCAA TTGAATATAT TTTCGAACGA GCTGCTGCCA TCCTTCGACG AGAGCCTCGA 
ACCACCCGTG CAGACTCTTT AATCAATAAA ACGGCTTGCA TGAAACTTCT CGCAGCTCTC 
TGCACTCATC TTGACGTCGC TCAAATTACT CCGTCCCTCC AGACAATCCT TCTTCCGTTA 
ATACATCTAA CCGACCCATC GATCCCTGCG CCTCGCTCCC TCGATGAGCA GTTCCAGGCT 
ACTTACAAAG CCCTTGTTAG CAGCAGCCAG GAGATCCTCG ATTTGTTACA GAAGAAGTTA 
GGTACGACTG ATTTCGTGAC GCAAATGGCG GCGGTTCGTG AGAGTGTCAA GTCAAGACGG 
GAGGGTAGAA GAGTTAAGAG GCGCATCGAA GCTGTTGCTG ATCCTGAGAA GTTCGGCAGA 
GAAAAGAAGA GGAAGAATGA CCGGAAGCGA GAGAAGAGGA AGGAAAAGGG CGCAGAATAT 
CAAGATCGGA GACGTGGCTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BDBG_03360 ( XM_031721215.1 ) cDNA ORF clone, Blastomyces gilchristii SLH14081 -> XP_031577687.1 Blastomyces gilchristii SLH14081 HEAT repeat protein (BDBG_03360), mRNA.

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CloneID	OAl115268
Clone ID Related Accession (Same CDS sequence)	XM_031721215.1
Accession Version	XM_031721215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574697600000
Organism	Blastomyces gilchristii SLH14081
Product	HEAT repeat protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101678).

ATGGCGACAG CACATTCTGG GCGCCAGGGC AAGCCTGCCA AGCTCTTAAA GAAGGGCACA 
GCTACGACAA AATCCCACCG ATTCGAGTCT TTCTCCCAGC GCGTCGCCAA ATTAAAAATC 
GATCCTATCC ATCGTGTGCG ACGGGCCAGC GTTGGCGATG ACAGCGACTC TGAGTCCGAT 
TCGTACTTCA GGGCCTCCCT GGAGCACTGG GTGGAACTTA ACCTGTCAGA AAACTTCACT 
CAGTTCTCCC AGCGAGTTAA TCGGCTTTGC GAAAGCTTAC CGCAGATTCT GCATCATGAA 
GATCGAATAA TGGGATTATT GGCCGAGTAT ATCGGGAAAA GAGATGAGTT GAGCATGGAA 
CCGCTGCTGA GTCTTGTAGC TCAGTTTGCA CGAGACTTGG GCCAGCGATT CGAAAGACAC 
TTTGCGTCGA CCGTCACGTT GGTGGCCTCC GTCGCAGCCA CACATCCAAA TATCGAGGTT 
ATAGAATGGA GTTTTACATG TCTGGCATGG ATTTTCAAGT TCCTGTCCCG CCTCCTTGTT 
CCGGACTTAC GCCAACTACT CGGGATAATG TCCCCGTACC TTGGGAAGGA AAAGCAAAAA 
CACTTTGTGA CAAGATTTGC TGCGGAGTCG ATGTCCTTCC TGGTTCGGAA GGCCGCGCTT 
GTCTACTACA AAAATAAGGC GCCACTCGAC AGAGCCGTTA CGTTTCTTTT TGAGGATCTC 
GCCAGCACTG ATGGGGAACG TCAGATTTCA ATGTACCAAC AAGGCCTTAT GTCAATGTTT 
TCTGATGCGA TTAAAGGAGT GAATGGTGGT ATCCATTCGA ATGGAGCTGA CATCATACGT 
TGCCTAATCA GTGCCGCCGC TGTGAAAGAT GAAGCCCAAA GCGCCCTCGC AGAGCAGGTT 
CTTAGTGGGG TTGTAATTAA TTTGGTTCAT CACACATCCT CAAACACCTT TGGTTCGGTG 
CTTGATGTGA TCTGTGATCA CATTGGATCC GCTAAACCGA ATGGTAACAT TGCAGCGACG 
AAGATTGACA TCCGCTTGAT TTTTGTTGCC GTAACCGCGC GCAAGGGTTC TAGAGTTAGC 
AACTGGAAAA GAGTACATCA AACTCTCATG ACCCTTCTTG GTCGCATCGC TAAATCGCCC 
AATATCCCGG CGGATTCAGT TCAACTCCTT TTAGCTTCCG TGGTGACTGC CGTGCATACG 
TCCCCGATGG ACGAATTGCT GTCGTTTATG CGTCCTATTA TGGAGTTAAT TAGCGGCGAC 
CGATTGCGGG ACTACTTCCT CCCGTTTTGC TCGTTATTCT CTACTTTCGG GACCGATCGA 
TTTCAAAGCG TTGTACTCCC ATACTTTCAG CGGTTCATTA CTTCGTTCTG GAAGGATTAC 
GAATCTGGAC TTTGCCAGAT ATTACCCAAA CTTCATGCCG CCCATTTCGT CACGTCTCAA 
CCTAACAGGC CCGGATCTTT AAATTGCCCT GGTGGCTGGA AGGAGGCTAT CGTGGAAAGA 
TTCAGCAAAC TCGATCCCAC AGCCGAGGAT ATCGCTTTCC TACATTCCTA TACCAAACTC 
CATGAGGCTA TATCACTCAC TTCCGAGCCC TCGATTCTTC CACAGCTAAC CCACTGCCTC 
CACGATCGGT TAAAGATTGC TATGGCACTC TCCCCCAACG TTGGAGAGGG CCAGGTCACA 
TTTGCCCGAG GCCAAGGGTT TTTGGCTTAC GTCCGCCTTG CTACGGAACT AGGCTCACTT 
GATTTAGGTC TTTGGAGTAT GATAGTGTCC AACGGCCCTG AGTGCTCAAA TTGTCCGGTT 
TTCCTTGAAG CAACCTTGGC TTATTTGTCT GCTTGTACCA AATACGAGCA GGTCCCGGAC 
GCAGACCTTG AACCATTTGC TACAACTCTG ATTAATAACC TTTCTGGCCC ATCGCACGAC 
ATCAGACAAT TGTCACTCAA GATTCTCCAA GTTTTATTGT CCAGGGTGAA CGATAGCGAC 
AGTACTTGCA TAGAAATAGC GATCGAAATC GAACAGAGCG AGCTTACTCT GCATGCATCA 
AGGGCTCTAT CCATGCAGGT ACGCAAACTT GCGATTGCAT ACCCAACGGT GTGTTCCAAA 
AAGTGGTTGG ATCGAATTAT TCCAAATTTC TGTTTTGGTT TGCTATCTAA ACGGCTCGCG 
TCGCTGTGGG ATGACGCATG TGAGGCAATC AAGGCAATTT GCGAGGTTCC AAATGGAGAA 
AGCATTGTTA CGGAGCTCGC CATGCGCGGG CTACAAGAGC CTAGTCCGCT TGGAACCAAT 
GCAAATACCC CCGAAGAAGA ATCCGCTGAA TCGCATATTT CGTCGGAATT CGAATGCTTT 
AACGTTTCAA ATGTCGAGAA AACTATGTGT GCGACTTTCG CTGACTCCCG CAACTCTAAT 
TCGTTGCTCG CAGAAGATTT CAAGCGTGCT CATGTTACGG AACCTCAACG TTCTACTTCT 
AGTCGAACAC AGGCCCTCAA GGTCCTGGGA TCTGTACCTC AAGTGGCGGA GAAACGATCT 
CGGCAATTTG TTCCTCTATT CCTGATATGG GCAGCTCATG ATGATGACAT CCCTACCGCC 
AACGAATCCA AGCCTTCTGG TACAAGTGGC ACAGATGATA GTTCTGTTTC TTCCTGGAAT 
CTGCGTGACC GGAAAGCTAT GCTGGACATC TTCGGAAAAT TCGTCAACCC CAAAGTTTTA 
TACAAAGCAG CAGAGGTCCA TGAAGCTTTG CTTGGCCTTC TCGGTAATGG AGATACTGAA 
ATTCAAAAAT CTGCACTGAA GGCTATTTTC ACATGGAAGT CACCTGCAAT TCAACCTTAC 
GAGACGAACA TTTTGAATAT TGTGGATGAT GCGAGATTCC GAGATGAACT ATCAGTGTTC 
TTAAATGCTG GCCAGGAGGC TAGTACGATT AAGGAAAATC ATCGTGCTGA GCTATTCCCT 
GTTCTGCTGA GACTTCTATA TGGCAAGATT GTCGCCCGTG GGGGCTCTAG GGGTGGACAG 
GGGACCCAAG AGGCACGCAG AAAGACCATT TTAAGAACAA TATCCCAACT CTCTGAAAGC 
GACTTTGGTC ACTTCGTTCA AATAGCGTTT GGTCCCCTGA GCGATATTCG CGTCCTCGAA 
ACCGACCCTG GACATGGCCA GTGCCTCAAT CAGGAGGTGA TGGGTGTTAG AAAGCAATAT 
GGCCTTCTGA AGATGGTGGA AACTATGTTC GAAACCCTGA AAACCAAGAT GTCACCTTTC 
GTTGACCGCT CCATGAACGT AGTTCTATAC TGTCTTGTCA GAGCTTGCAG GCAGTTGAAC 
GATGCCAGTT CAGAAGAGAA TCATTCGTCT CTCATCCGCA ATACTCGGCA GACAGGTGTC 
CATTGTCTCG ATCTGATATT TTCTGTTTCG CCCGATTATG ATTGGACTCC ATATATACCC 
CTGATTTTAA ACGAGATCAT CAGCCCAAGA TTAGGAAATT TCGCCGTGGA AACAGCGCAA 
AGTGTGTCCG GCCTTCTTCA TCTGTTCCAC ACCTGGGCGT CCAGCCCCAA GTCAGCTCTC 
TACTTCCTGC ATGCTGACCA ACAGGTCATT CAACGAATTG TTGACTGCCT TGGCGTTGAA 
TCTGCTCGCG AAGACGTCAA GATATTTGTT CTGAATGAAA TTCTTAACAA CCTCGTCAGT 
CGCGCGAATG GCGAGACAGA CGACCCAGAG AATTTGCCAG ATGCCGCAAC AACAGATCTA 
ATCCGGTCTA AAGTGCTGGC ACCCAATGTT GAATACATAT TGACACATGT CGAATCCCTG 
CTCAGGAATC AATCTACGCG GCAACTTACG ATGGCTGCAA TTGAGACCCT ATCTAAACTC 
GCGCCGTTTG TCCAGTCATC GCAAGAAACC ACGAAACTAA TTAGTACGGC TACATTTCTC 
CTTAACGAAC CTGCAGATCG AGTACCCCCC AAAGCCAAAA GCGGACTTCT GCGTGTTATG 
CATCATTTCC TCCCCCTATA TGATCCACGC CAAAATGTGG ACCTCAACCA GCGAATCTTT 
GAAGTAATTT CCTCCCTATT CGATTACTTC AAAGATAACC CGAACCGTGC TATGCTCTCA 
TCCGTGTTGT CTGCATTTGC AAACCACGAA GTGGACCTAA AAGGTGTTGC CGAATTATGT 
GCAGACCTCA ACGCTATATC AGAGAAAAAT CTTGATGAAT TAGATTTCGA CCGACGGCTT 
AGGGCATTTA ACACAATAAA CGAACAGAAG TACAAGGAGT TCACAGCTAG GCAATGGCGC 
CCGCTGTTAT TCAATCTTCT ATACCATGTT AAAGACGAGG AGGAACTTGC AATTCGATCA 
AGTTCATCAT TTGGACTGAA AAGGTTTATC GAGAGCGTGG GTTCAAAACC AGAGGGTGAT 
AGCGAAGACT ACTCATTTTT GCTCGAGAAA GCTTTGTTGC CTGCGTTGAG GAACGGAGCA 
AAACACCCTG CCGAGACAGT TCGGGCCGAG TTCGTCTCTG TCTTGGGTTA TTTCATAACG 
CATAACCCGA ACCATCGGGC CGTGACCGAC ATGGTGGACT TACTAGCAGG TGGGGATGAA 
GAGGCATCGT TTTTCAATAA TATTCTCCAT ATTCAACAAC ATCGTCGTCT TCGTGCCTTG 
CGGCGACTGG CTACTGAGGC TAGACAAGGA AAGTTTGAGT CATCAAATAT TAGCTCCTTT 
TTCTTACCTC TTATTGAACA TTATGTGTTT GATCAGGCAG AAGACGATAG TGCCCATAAC 
CTGGCCGCGG AGACAGTTAC AGCTATTGGG GTGCTGTCTG AAGGGCTGGA GTGGAGCCAG 
TTTAGAGCGA TATTCCGCCG ATACAAGGGA TATGTACGAA GTCGCCCTGG GATGGAAAAG 
AACGTTATAC GTCTTCTAGG ACAGATGACC GATGGCTTGT CTCGAGCAAT GAGTATTGAT 
AAAGAAGAAC AGCCGGTTGC TGGGGATGGA GAAATGGAAG GAATTGAGAA AGAGTCTACG 
ATTAAAAGCA CGCTCTCGAA GACTTTGCCT GCGGAGGCTC GCATCGGGAC AGAACTGAAA 
ACCAACTTCA TCCCTTTCTT ATCCGAGTTT GTACACCAGA AGGATGAATC CGAAGTTCAT 
CTCCGTGTCC CAGTCGCAGT CATAACTGTT AAGTTACTCA AACTTCTTCC TAAGGATGAA 
ATGGCACTTC TTCTCCCATC GGTACTGCTA GACCTATCGA ACGTTCTTAG AAGTCGGTCT 
CAGGATGCTA GGGATATGGC CAGAAAAACA CTCGCTGATG TCGCGCTTAT TCTTGGACCT 
TCTTACTTTG GATATATTCT GAGAGAATTG CGGAGCACGT TAACAAGAGG ATACCAACTC 
CATGTGCTGT CCTTTACAGT CCATTCGATT CTTGTAGCCA CCAGCGACCA TTTCGAGCCT 
GGTGACCTCG ATCACAGTCT CGACGCACTA GCAGCCGTGG TGATGGATGA TATTTTCGGC 
ACCGTCGGCC AAGAGAAGGA CGCCGAGGAG TACGTGAGCA AAATGAAGGA GGTAAAGAGT 
AACAAAAGCT ATGATTCAAT GGAGCTACTC GCGAAAATAT CGACAATCCG CCATCTCTCG 
GCTCTTATTA ACCCAGTGCA AGCGCTTTTG CGAGAGAAAC TTACGGCTAC CATCATCAGA 
AAAGTCGACG AACTGCTTAG ACGGATCGGT GTCGGCCTGC TGCGGAATCC TGGAGCTGAA 
AGCCGTGATC TTTTGGTTTT CTGCTACGAG GTGATAAAGG AGTCATACAA AGCAGACACA 
CCACAAATTG AAACACAGGA GCAAGGTAAC AGGGCCCGTT TCATCGTCAA TTTGCTTAAC 
CCGAGGAACC GTATGAATCG AGGAAGCACA TCTTCCTATT CCTACAAGCT TTCTCGCTTC 
GGACTTGATG TTCTCCGCTC CGTGCTAAAT AAGTACCAAT CTCTCATGAC AGCCGCCAAC 
GTTGCAGGGT TCCTTCCCAT TATTGGAGAT GCATTGCTAC AGAGCTATGA GGAGGTCAAA 
CTGTCTGCCA TTAGACTCAT ATCCACGATC ATCAAAGTCC CACTTTCCGA GCTCGATAGA 
AACTCTGATG TATATCTAAT GGAAGCTATT AAGTTGGTCA AAGAAGCTCC GAACACAAAT 
ACCGAAGCTG CCCAAGCTGC CTTGAAGCTC ATCGCTTCCA TCCTACGCGA GCGTAAGAGT 
ACAAAGCTCA AGGACTCCCA CCTGGGCTAT TTGTTGAAAC GAATAGCTGC TGACATCGAG 
GAGCCTGACC GTCAAGGAGT GGCTTTCAAT TTCATTCGGG CAGTGATGTC TCGCAAATTT 
ATTGTTCCCG AGATGTACGA GCTTGTCGAT AATGTTGCTG CGATGATGGT AACAAACCAC 
ACAAGAAGTG CTCGCGATCT CGCAAGAGGT GTCTTTATAC ACTTTATGAT TGAATACCCT 
CAAGCTAAAA GCAGGTGGAC GAAGCAACTT GGATTCCTCG CCAAAAACCT TGACTATCAA 
CATAAAGAAG GCCGTCAGTC CGTTATGGAA GCAGTTCATC TTCTTCTCTC CAAGACCAAT 
GGCGACCCTG CTCAGGATAT TGTGGGTAAC TTTTTTGTGC CAGTGGTAAT GGTAATGTCT 
AATGATGAGG CAGTTGAATG TCGAGAGATG GCTGGTGTTC TTCTTAATGA TCTCTTCAGC 
CGTGCTGATT CAGAGCAGCT AAAGTCTATG CTCTCTCCTC TTCGCTCCTG GTTGGAGCAA 
TCCCAAAACA CACTGCTCAC TGCCACTGGG TTACAAGCGA TCAGGATATT CTTTGAGTCG 
CATAGGAACA ATAAAGAAGG CGAAATGCGT TTTGTTACGG ATTTATTGCC TCGAATCCTT 
GGCCCCGCAG TGGAGAACTG CGAAGGGGAA GAGTGGGAAG TCCTCTACCA TTCGCTACAG 
CTCTTTACAA AACTATGCCA GCTCTATCCA GCGGTTGCCA TGTCCGAGGA TTGCGAGAAC 
ATCTGGCGCT ATGTTTGCGA AAGCCTCTTC TATCCACACG CGTGGATCAA GTCATGCGCA 
GCAAATTTAA TTGGAATATG GCTTGCAGAC GAAGCGAAAG GAAATGCCGT TACTGGTTAT 
GGCTCTGTCC CACTCATCGG CGCATCTGGT CTTAAGCTCG AAGGCTGCAT GATGTTAGAT 
ATTACGCGAG CCAGTCTGCT ATGTCTGAAA TCAACCACTA TTTCCGAAGA TCTCGCAACT 
CAGACAATCC GGAATCTGGT GTTCTTAGGA CGCTGCTTCA GCCAGAATAA CCTTGCGTTT 
TCGAAGAAGG CTGAGGAGCT GGCTGGTGAT TTCAGCGACG ACAGCGACGA TGAAGACAAC 
ATTGACCCAG CTACGGTGAA TTCCGCAAAG CAAAACAAGC CAGCAATTGA ATATATTTTC 
GAACGAGCTG CTGCCATCCT TCGACGAGAG CCTCGAACCA CCCGTGCAGA CTCTTTAATC 
AATAAAACGG CTTGCATGAA ACTTCTCGCA GCTCTCTGCA CTCATCTTGA CGTCGCTCAA 
ATTACTCCGT CCCTCCAGAC AATCCTTCTT CCGTTAATAC ATCTAACCGA CCCATCGATC 
CCTGCGCCTC GCTCCCTCGA TGAGCAGTTC CAGGCTACTT ACAAAGCCCT TGTTAGCAGC 
AGCCAGGAGA TCCTCGATTT GTTACAGAAG AAGTTAGGTA CGACTGATTT CGTGACGCAA 
ATGGCGGCGG TTCGTGAGAG TGTCAAGTCA AGACGGGAGG GTAGAAGAGT TAAGAGGCGC 
ATCGAAGCTG TTGCTGATCC TGAGAAGTTC GGCAGAGAAA AGAAGAGGAA GAATGACCGG 
AAGCGAGAGA AGAGGAAGGA AAAGGGCGCA GAATATCAAG ATCGGAGACG TGGCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031577687.1
CDS	99..7958
Translation	MATAHSGRQGKPAKLLKKGTATTKSHRFESFSQRVAKLKIDPIHRVRRASVGDDSDSESDSYFRASLEHWVELNLSENFTQFSQRVNRLCESLPQILHHEDRIMGLLAEYIGKRDELSMEPLLSLVAQFARDLGQRFERHFASTVTLVASVAATHPNIEVIEWSFTCLAWIFKFLSRLLVPDLRQLLGIMSPYLGKEKQKHFVTRFAAESMSFLVRKAALVYYKNKAPLDRAVTFLFEDLASTDGERQISMYQQGLMSMFSDAIKGVNGGIHSNGADIIRCLISAAAVKDEAQSALAEQVLSGVVINLVHHTSSNTFGSVLDVICDHIGSAKPNGNIAATKIDIRLIFVAVTARKGSRVSNWKRVHQTLMTLLGRIAKSPNIPADSVQLLLASVVTAVHTSPMDELLSFMRPIMELISGDRLRDYFLPFCSLFSTFGTDRFQSVVLPYFQRFITSFWKDYESGLCQILPKLHAAHFVTSQPNRPGSLNCPGGWKEAIVERFSKLDPTAEDIAFLHSYTKLHEAISLTSEPSILPQLTHCLHDRLKIAMALSPNVGEGQVTFARGQGFLAYVRLATELGSLDLGLWSMIVSNGPECSNCPVFLEATLAYLSACTKYEQVPDADLEPFATTLINNLSGPSHDIRQLSLKILQVLLSRVNDSDSTCIEIAIEIEQSELTLHASRALSMQVRKLAIAYPTVCSKKWLDRIIPNFCFGLLSKRLASLWDDACEAIKAICEVPNGESIVTELAMRGLQEPSPLGTNANTPEEESAESHISSEFECFNVSNVEKTMCATFADSRNSNSLLAEDFKRAHVTEPQRSTSSRTQALKVLGSVPQVAEKRSRQFVPLFLIWAAHDDDIPTANESKPSGTSGTDDSSVSSWNLRDRKAMLDIFGKFVNPKVLYKAAEVHEALLGLLGNGDTEIQKSALKAIFTWKSPAIQPYETNILNIVDDARFRDELSVFLNAGQEASTIKENHRAELFPVLLRLLYGKIVARGGSRGGQGTQEARRKTILRTISQLSESDFGHFVQIAFGPLSDIRVLETDPGHGQCLNQEVMGVRKQYGLLKMVETMFETLKTKMSPFVDRSMNVVLYCLVRACRQLNDASSEENHSSLIRNTRQTGVHCLDLIFSVSPDYDWTPYIPLILNEIISPRLGNFAVETAQSVSGLLHLFHTWASSPKSALYFLHADQQVIQRIVDCLGVESAREDVKIFVLNEILNNLVSRANGETDDPENLPDAATTDLIRSKVLAPNVEYILTHVESLLRNQSTRQLTMAAIETLSKLAPFVQSSQETTKLISTATFLLNEPADRVPPKAKSGLLRVMHHFLPLYDPRQNVDLNQRIFEVISSLFDYFKDNPNRAMLSSVLSAFANHEVDLKGVAELCADLNAISEKNLDELDFDRRLRAFNTINEQKYKEFTARQWRPLLFNLLYHVKDEEELAIRSSSSFGLKRFIESVGSKPEGDSEDYSFLLEKALLPALRNGAKHPAETVRAEFVSVLGYFITHNPNHRAVTDMVDLLAGGDEEASFFNNILHIQQHRRLRALRRLATEARQGKFESSNISSFFLPLIEHYVFDQAEDDSAHNLAAETVTAIGVLSEGLEWSQFRAIFRRYKGYVRSRPGMEKNVIRLLGQMTDGLSRAMSIDKEEQPVAGDGEMEGIEKESTIKSTLSKTLPAEARIGTELKTNFIPFLSEFVHQKDESEVHLRVPVAVITVKLLKLLPKDEMALLLPSVLLDLSNVLRSRSQDARDMARKTLADVALILGPSYFGYILRELRSTLTRGYQLHVLSFTVHSILVATSDHFEPGDLDHSLDALAAVVMDDIFGTVGQEKDAEEYVSKMKEVKSNKSYDSMELLAKISTIRHLSALINPVQALLREKLTATIIRKVDELLRRIGVGLLRNPGAESRDLLVFCYEVIKESYKADTPQIETQEQGNRARFIVNLLNPRNRMNRGSTSSYSYKLSRFGLDVLRSVLNKYQSLMTAANVAGFLPIIGDALLQSYEEVKLSAIRLISTIIKVPLSELDRNSDVYLMEAIKLVKEAPNTNTEAAQAALKLIASILRERKSTKLKDSHLGYLLKRIAADIEEPDRQGVAFNFIRAVMSRKFIVPEMYELVDNVAAMMVTNHTRSARDLARGVFIHFMIEYPQAKSRWTKQLGFLAKNLDYQHKEGRQSVMEAVHLLLSKTNGDPAQDIVGNFFVPVVMVMSNDEAVECREMAGVLLNDLFSRADSEQLKSMLSPLRSWLEQSQNTLLTATGLQAIRIFFESHRNNKEGEMRFVTDLLPRILGPAVENCEGEEWEVLYHSLQLFTKLCQLYPAVAMSEDCENIWRYVCESLFYPHAWIKSCAANLIGIWLADEAKGNAVTGYGSVPLIGASGLKLEGCMMLDITRASLLCLKSTTISEDLATQTIRNLVFLGRCFSQNNLAFSKKAEELAGDFSDDSDDEDNIDPATVNSAKQNKPAIEYIFERAAAILRREPRTTRADSLINKTACMKLLAALCTHLDVAQITPSLQTILLPLIHLTDPSIPAPRSLDEQFQATYKALVSSSQEILDLLQKKLGTTDFVTQMAAVRESVKSRREGRRVKRRIEAVADPEKFGREKKRKNDRKREKRKEKGAEYQDRRRGW

Target ORF information:

RefSeq Version	XM_031721215.1
Organism	Blastomyces gilchristii SLH14081
Definition	Blastomyces gilchristii SLH14081 HEAT repeat protein (BDBG_03360), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031721215.1

ORF Insert Sequence:

ATGGCGACAG CACATTCTGG GCGCCAGGGC AAGCCTGCCA AGCTCTTAAA GAAGGGCACA 
GCTACGACAA AATCCCACCG ATTCGAGTCT TTCTCCCAGC GCGTCGCCAA ATTAAAAATC 
GATCCTATCC ATCGTGTGCG ACGGGCCAGC GTTGGCGATG ACAGCGACTC TGAGTCCGAT 
TCGTACTTCA GGGCCTCCCT GGAGCACTGG GTGGAACTTA ACCTGTCAGA AAACTTCACT 
CAGTTCTCCC AGCGAGTTAA TCGGCTTTGC GAAAGCTTAC CGCAGATTCT GCATCATGAA 
GATCGAATAA TGGGATTATT GGCCGAGTAT ATCGGGAAAA GAGATGAGTT GAGCATGGAA 
CCGCTGCTGA GTCTTGTAGC TCAGTTTGCA CGAGACTTGG GCCAGCGATT CGAAAGACAC 
TTTGCGTCGA CCGTCACGTT GGTGGCCTCC GTCGCAGCCA CACATCCAAA TATCGAGGTT 
ATAGAATGGA GTTTTACATG TCTGGCATGG ATTTTCAAGT TCCTGTCCCG CCTCCTTGTT 
CCGGACTTAC GCCAACTACT CGGGATAATG TCCCCGTACC TTGGGAAGGA AAAGCAAAAA 
CACTTTGTGA CAAGATTTGC TGCGGAGTCG ATGTCCTTCC TGGTTCGGAA GGCCGCGCTT 
GTCTACTACA AAAATAAGGC GCCACTCGAC AGAGCCGTTA CGTTTCTTTT TGAGGATCTC 
GCCAGCACTG ATGGGGAACG TCAGATTTCA ATGTACCAAC AAGGCCTTAT GTCAATGTTT 
TCTGATGCGA TTAAAGGAGT GAATGGTGGT ATCCATTCGA ATGGAGCTGA CATCATACGT 
TGCCTAATCA GTGCCGCCGC TGTGAAAGAT GAAGCCCAAA GCGCCCTCGC AGAGCAGGTT 
CTTAGTGGGG TTGTAATTAA TTTGGTTCAT CACACATCCT CAAACACCTT TGGTTCGGTG 
CTTGATGTGA TCTGTGATCA CATTGGATCC GCTAAACCGA ATGGTAACAT TGCAGCGACG 
AAGATTGACA TCCGCTTGAT TTTTGTTGCC GTAACCGCGC GCAAGGGTTC TAGAGTTAGC 
AACTGGAAAA GAGTACATCA AACTCTCATG ACCCTTCTTG GTCGCATCGC TAAATCGCCC 
AATATCCCGG CGGATTCAGT TCAACTCCTT TTAGCTTCCG TGGTGACTGC CGTGCATACG 
TCCCCGATGG ACGAATTGCT GTCGTTTATG CGTCCTATTA TGGAGTTAAT TAGCGGCGAC 
CGATTGCGGG ACTACTTCCT CCCGTTTTGC TCGTTATTCT CTACTTTCGG GACCGATCGA 
TTTCAAAGCG TTGTACTCCC ATACTTTCAG CGGTTCATTA CTTCGTTCTG GAAGGATTAC 
GAATCTGGAC TTTGCCAGAT ATTACCCAAA CTTCATGCCG CCCATTTCGT CACGTCTCAA 
CCTAACAGGC CCGGATCTTT AAATTGCCCT GGTGGCTGGA AGGAGGCTAT CGTGGAAAGA 
TTCAGCAAAC TCGATCCCAC AGCCGAGGAT ATCGCTTTCC TACATTCCTA TACCAAACTC 
CATGAGGCTA TATCACTCAC TTCCGAGCCC TCGATTCTTC CACAGCTAAC CCACTGCCTC 
CACGATCGGT TAAAGATTGC TATGGCACTC TCCCCCAACG TTGGAGAGGG CCAGGTCACA 
TTTGCCCGAG GCCAAGGGTT TTTGGCTTAC GTCCGCCTTG CTACGGAACT AGGCTCACTT 
GATTTAGGTC TTTGGAGTAT GATAGTGTCC AACGGCCCTG AGTGCTCAAA TTGTCCGGTT 
TTCCTTGAAG CAACCTTGGC TTATTTGTCT GCTTGTACCA AATACGAGCA GGTCCCGGAC 
GCAGACCTTG AACCATTTGC TACAACTCTG ATTAATAACC TTTCTGGCCC ATCGCACGAC 
ATCAGACAAT TGTCACTCAA GATTCTCCAA GTTTTATTGT CCAGGGTGAA CGATAGCGAC 
AGTACTTGCA TAGAAATAGC GATCGAAATC GAACAGAGCG AGCTTACTCT GCATGCATCA 
AGGGCTCTAT CCATGCAGGT ACGCAAACTT GCGATTGCAT ACCCAACGGT GTGTTCCAAA 
AAGTGGTTGG ATCGAATTAT TCCAAATTTC TGTTTTGGTT TGCTATCTAA ACGGCTCGCG 
TCGCTGTGGG ATGACGCATG TGAGGCAATC AAGGCAATTT GCGAGGTTCC AAATGGAGAA 
AGCATTGTTA CGGAGCTCGC CATGCGCGGG CTACAAGAGC CTAGTCCGCT TGGAACCAAT 
GCAAATACCC CCGAAGAAGA ATCCGCTGAA TCGCATATTT CGTCGGAATT CGAATGCTTT 
AACGTTTCAA ATGTCGAGAA AACTATGTGT GCGACTTTCG CTGACTCCCG CAACTCTAAT 
TCGTTGCTCG CAGAAGATTT CAAGCGTGCT CATGTTACGG AACCTCAACG TTCTACTTCT 
AGTCGAACAC AGGCCCTCAA GGTCCTGGGA TCTGTACCTC AAGTGGCGGA GAAACGATCT 
CGGCAATTTG TTCCTCTATT CCTGATATGG GCAGCTCATG ATGATGACAT CCCTACCGCC 
AACGAATCCA AGCCTTCTGG TACAAGTGGC ACAGATGATA GTTCTGTTTC TTCCTGGAAT 
CTGCGTGACC GGAAAGCTAT GCTGGACATC TTCGGAAAAT TCGTCAACCC CAAAGTTTTA 
TACAAAGCAG CAGAGGTCCA TGAAGCTTTG CTTGGCCTTC TCGGTAATGG AGATACTGAA 
ATTCAAAAAT CTGCACTGAA GGCTATTTTC ACATGGAAGT CACCTGCAAT TCAACCTTAC 
GAGACGAACA TTTTGAATAT TGTGGATGAT GCGAGATTCC GAGATGAACT ATCAGTGTTC 
TTAAATGCTG GCCAGGAGGC TAGTACGATT AAGGAAAATC ATCGTGCTGA GCTATTCCCT 
GTTCTGCTGA GACTTCTATA TGGCAAGATT GTCGCCCGTG GGGGCTCTAG GGGTGGACAG 
GGGACCCAAG AGGCACGCAG AAAGACCATT TTAAGAACAA TATCCCAACT CTCTGAAAGC 
GACTTTGGTC ACTTCGTTCA AATAGCGTTT GGTCCCCTGA GCGATATTCG CGTCCTCGAA 
ACCGACCCTG GACATGGCCA GTGCCTCAAT CAGGAGGTGA TGGGTGTTAG AAAGCAATAT 
GGCCTTCTGA AGATGGTGGA AACTATGTTC GAAACCCTGA AAACCAAGAT GTCACCTTTC 
GTTGACCGCT CCATGAACGT AGTTCTATAC TGTCTTGTCA GAGCTTGCAG GCAGTTGAAC 
GATGCCAGTT CAGAAGAGAA TCATTCGTCT CTCATCCGCA ATACTCGGCA GACAGGTGTC 
CATTGTCTCG ATCTGATATT TTCTGTTTCG CCCGATTATG ATTGGACTCC ATATATACCC 
CTGATTTTAA ACGAGATCAT CAGCCCAAGA TTAGGAAATT TCGCCGTGGA AACAGCGCAA 
AGTGTGTCCG GCCTTCTTCA TCTGTTCCAC ACCTGGGCGT CCAGCCCCAA GTCAGCTCTC 
TACTTCCTGC ATGCTGACCA ACAGGTCATT CAACGAATTG TTGACTGCCT TGGCGTTGAA 
TCTGCTCGCG AAGACGTCAA GATATTTGTT CTGAATGAAA TTCTTAACAA CCTCGTCAGT 
CGCGCGAATG GCGAGACAGA CGACCCAGAG AATTTGCCAG ATGCCGCAAC AACAGATCTA 
ATCCGGTCTA AAGTGCTGGC ACCCAATGTT GAATACATAT TGACACATGT CGAATCCCTG 
CTCAGGAATC AATCTACGCG GCAACTTACG ATGGCTGCAA TTGAGACCCT ATCTAAACTC 
GCGCCGTTTG TCCAGTCATC GCAAGAAACC ACGAAACTAA TTAGTACGGC TACATTTCTC 
CTTAACGAAC CTGCAGATCG AGTACCCCCC AAAGCCAAAA GCGGACTTCT GCGTGTTATG 
CATCATTTCC TCCCCCTATA TGATCCACGC CAAAATGTGG ACCTCAACCA GCGAATCTTT 
GAAGTAATTT CCTCCCTATT CGATTACTTC AAAGATAACC CGAACCGTGC TATGCTCTCA 
TCCGTGTTGT CTGCATTTGC AAACCACGAA GTGGACCTAA AAGGTGTTGC CGAATTATGT 
GCAGACCTCA ACGCTATATC AGAGAAAAAT CTTGATGAAT TAGATTTCGA CCGACGGCTT 
AGGGCATTTA ACACAATAAA CGAACAGAAG TACAAGGAGT TCACAGCTAG GCAATGGCGC 
CCGCTGTTAT TCAATCTTCT ATACCATGTT AAAGACGAGG AGGAACTTGC AATTCGATCA 
AGTTCATCAT TTGGACTGAA AAGGTTTATC GAGAGCGTGG GTTCAAAACC AGAGGGTGAT 
AGCGAAGACT ACTCATTTTT GCTCGAGAAA GCTTTGTTGC CTGCGTTGAG GAACGGAGCA 
AAACACCCTG CCGAGACAGT TCGGGCCGAG TTCGTCTCTG TCTTGGGTTA TTTCATAACG 
CATAACCCGA ACCATCGGGC CGTGACCGAC ATGGTGGACT TACTAGCAGG TGGGGATGAA 
GAGGCATCGT TTTTCAATAA TATTCTCCAT ATTCAACAAC ATCGTCGTCT TCGTGCCTTG 
CGGCGACTGG CTACTGAGGC TAGACAAGGA AAGTTTGAGT CATCAAATAT TAGCTCCTTT 
TTCTTACCTC TTATTGAACA TTATGTGTTT GATCAGGCAG AAGACGATAG TGCCCATAAC 
CTGGCCGCGG AGACAGTTAC AGCTATTGGG GTGCTGTCTG AAGGGCTGGA GTGGAGCCAG 
TTTAGAGCGA TATTCCGCCG ATACAAGGGA TATGTACGAA GTCGCCCTGG GATGGAAAAG 
AACGTTATAC GTCTTCTAGG ACAGATGACC GATGGCTTGT CTCGAGCAAT GAGTATTGAT 
AAAGAAGAAC AGCCGGTTGC TGGGGATGGA GAAATGGAAG GAATTGAGAA AGAGTCTACG 
ATTAAAAGCA CGCTCTCGAA GACTTTGCCT GCGGAGGCTC GCATCGGGAC AGAACTGAAA 
ACCAACTTCA TCCCTTTCTT ATCCGAGTTT GTACACCAGA AGGATGAATC CGAAGTTCAT 
CTCCGTGTCC CAGTCGCAGT CATAACTGTT AAGTTACTCA AACTTCTTCC TAAGGATGAA 
ATGGCACTTC TTCTCCCATC GGTACTGCTA GACCTATCGA ACGTTCTTAG AAGTCGGTCT 
CAGGATGCTA GGGATATGGC CAGAAAAACA CTCGCTGATG TCGCGCTTAT TCTTGGACCT 
TCTTACTTTG GATATATTCT GAGAGAATTG CGGAGCACGT TAACAAGAGG ATACCAACTC 
CATGTGCTGT CCTTTACAGT CCATTCGATT CTTGTAGCCA CCAGCGACCA TTTCGAGCCT 
GGTGACCTCG ATCACAGTCT CGACGCACTA GCAGCCGTGG TGATGGATGA TATTTTCGGC 
ACCGTCGGCC AAGAGAAGGA CGCCGAGGAG TACGTGAGCA AAATGAAGGA GGTAAAGAGT 
AACAAAAGCT ATGATTCAAT GGAGCTACTC GCGAAAATAT CGACAATCCG CCATCTCTCG 
GCTCTTATTA ACCCAGTGCA AGCGCTTTTG CGAGAGAAAC TTACGGCTAC CATCATCAGA 
AAAGTCGACG AACTGCTTAG ACGGATCGGT GTCGGCCTGC TGCGGAATCC TGGAGCTGAA 
AGCCGTGATC TTTTGGTTTT CTGCTACGAG GTGATAAAGG AGTCATACAA AGCAGACACA 
CCACAAATTG AAACACAGGA GCAAGGTAAC AGGGCCCGTT TCATCGTCAA TTTGCTTAAC 
CCGAGGAACC GTATGAATCG AGGAAGCACA TCTTCCTATT CCTACAAGCT TTCTCGCTTC 
GGACTTGATG TTCTCCGCTC CGTGCTAAAT AAGTACCAAT CTCTCATGAC AGCCGCCAAC 
GTTGCAGGGT TCCTTCCCAT TATTGGAGAT GCATTGCTAC AGAGCTATGA GGAGGTCAAA 
CTGTCTGCCA TTAGACTCAT ATCCACGATC ATCAAAGTCC CACTTTCCGA GCTCGATAGA 
AACTCTGATG TATATCTAAT GGAAGCTATT AAGTTGGTCA AAGAAGCTCC GAACACAAAT 
ACCGAAGCTG CCCAAGCTGC CTTGAAGCTC ATCGCTTCCA TCCTACGCGA GCGTAAGAGT 
ACAAAGCTCA AGGACTCCCA CCTGGGCTAT TTGTTGAAAC GAATAGCTGC TGACATCGAG 
GAGCCTGACC GTCAAGGAGT GGCTTTCAAT TTCATTCGGG CAGTGATGTC TCGCAAATTT 
ATTGTTCCCG AGATGTACGA GCTTGTCGAT AATGTTGCTG CGATGATGGT AACAAACCAC 
ACAAGAAGTG CTCGCGATCT CGCAAGAGGT GTCTTTATAC ACTTTATGAT TGAATACCCT 
CAAGCTAAAA GCAGGTGGAC GAAGCAACTT GGATTCCTCG CCAAAAACCT TGACTATCAA 
CATAAAGAAG GCCGTCAGTC CGTTATGGAA GCAGTTCATC TTCTTCTCTC CAAGACCAAT 
GGCGACCCTG CTCAGGATAT TGTGGGTAAC TTTTTTGTGC CAGTGGTAAT GGTAATGTCT 
AATGATGAGG CAGTTGAATG TCGAGAGATG GCTGGTGTTC TTCTTAATGA TCTCTTCAGC 
CGTGCTGATT CAGAGCAGCT AAAGTCTATG CTCTCTCCTC TTCGCTCCTG GTTGGAGCAA 
TCCCAAAACA CACTGCTCAC TGCCACTGGG TTACAAGCGA TCAGGATATT CTTTGAGTCG 
CATAGGAACA ATAAAGAAGG CGAAATGCGT TTTGTTACGG ATTTATTGCC TCGAATCCTT 
GGCCCCGCAG TGGAGAACTG CGAAGGGGAA GAGTGGGAAG TCCTCTACCA TTCGCTACAG 
CTCTTTACAA AACTATGCCA GCTCTATCCA GCGGTTGCCA TGTCCGAGGA TTGCGAGAAC 
ATCTGGCGCT ATGTTTGCGA AAGCCTCTTC TATCCACACG CGTGGATCAA GTCATGCGCA 
GCAAATTTAA TTGGAATATG GCTTGCAGAC GAAGCGAAAG GAAATGCCGT TACTGGTTAT 
GGCTCTGTCC CACTCATCGG CGCATCTGGT CTTAAGCTCG AAGGCTGCAT GATGTTAGAT 
ATTACGCGAG CCAGTCTGCT ATGTCTGAAA TCAACCACTA TTTCCGAAGA TCTCGCAACT 
CAGACAATCC GGAATCTGGT GTTCTTAGGA CGCTGCTTCA GCCAGAATAA CCTTGCGTTT 
TCGAAGAAGG CTGAGGAGCT GGCTGGTGAT TTCAGCGACG ACAGCGACGA TGAAGACAAC 
ATTGACCCAG CTACGGTGAA TTCCGCAAAG CAAAACAAGC CAGCAATTGA ATATATTTTC 
GAACGAGCTG CTGCCATCCT TCGACGAGAG CCTCGAACCA CCCGTGCAGA CTCTTTAATC 
AATAAAACGG CTTGCATGAA ACTTCTCGCA GCTCTCTGCA CTCATCTTGA CGTCGCTCAA 
ATTACTCCGT CCCTCCAGAC AATCCTTCTT CCGTTAATAC ATCTAACCGA CCCATCGATC 
CCTGCGCCTC GCTCCCTCGA TGAGCAGTTC CAGGCTACTT ACAAAGCCCT TGTTAGCAGC 
AGCCAGGAGA TCCTCGATTT GTTACAGAAG AAGTTAGGTA CGACTGATTT CGTGACGCAA 
ATGGCGGCGG TTCGTGAGAG TGTCAAGTCA AGACGGGAGG GTAGAAGAGT TAAGAGGCGC 
ATCGAAGCTG TTGCTGATCC TGAGAAGTTC GGCAGAGAAA AGAAGAGGAA GAATGACCGG 
AAGCGAGAGA AGAGGAAGGA AAAGGGCGCA GAATATCAAG ATCGGAGACG TGGCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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BDBG_03360 cDNA ORF clone, Blastomyces gilchristii SLH14081

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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