bub1 gene cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

mRNA	Protein	Name
XM_015608507.1	XP_015463993.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
XM_015608507.2	XP_015463993.2	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
XM_015608508.2	XP_015463994.2	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X4
XM_007259885.3	XP_007259947.2	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
XM_007259886.3	XP_007259948.2	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
XM_015608508.1	XP_015463994.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X4
XM_007259886.2	XP_007259948.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
XM_007259887.2	XP_007259949.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X5
XM_007259885.2	XP_007259947.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
XM_007259887.3	XP_007259949.2	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAs123416	XM_007259885.3 Latest version!	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs123417	XM_015608507.2 Latest version!	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs123418	XM_007259886.3 Latest version!	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs123419	XM_015608508.2 Latest version!	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs123420	XM_007259887.3 Latest version!	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs41539	XM_007259885.2 Latest version!	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs41540	XM_015608507.1 Latest version!	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs41541	XM_007259886.2 Latest version!	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs41542	XM_015608508.1 Latest version!	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAs41543	XM_007259887.2 Latest version!	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

bub1 ( XM_007259885.3 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007259947.2 Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAs123416
Clone ID Related Accession (Same CDS sequence)	XM_007259885.3
Accession Version	XM_007259885.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_007259885.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CAACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCCTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGCGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACACATCTC ACGGAGGCTT TAATCATTCT 
CATGTCACTC CTAACACATC TCTGGGTCTG ATTTCGGCGA CTCCTTCACG TGCACTCCCC 
TCTCCCACTG TCAACACTCG GGAGGCTCTG GACGTGATCA TGGACATGTT CCAGGCTCCA 
ACGCTGCTGC AGGACTCCGT CTTCAATACT ACCAGCCAGG TGGCTGATGA CAGCTTTGAG 
AAAAACTGCA GAGTATACGG TTTTGCCCCA TCTGTAGCTA ATCCACCCTC CTCAATGTCG 
TTCAGGATAT ACCAGGATGA AAATGTGAAG ATTGGAAGTG AGGAACAACA GGTGGAGAAG 
AGCAAACCAG CAGCTCGAGC CCTGATGGAG ATTCCGGTCT CCAAACCCAA TGTCACTCCT 
GTAGGTCCGG AGTCTCTGAC AGACGAGAGT GCGATGTGGG GTCCTCAATA TGCCTCCATA 
ACTGCCTGTC CAAATCATAC TCGTGATTTT GCCATGGCTG CCCACCTGGT ATCCACCCCC 
CTCCACCCCA CTGCCCCGTA CACCTGGGAC ACAGAGCTCA ATCAGGTAGA AAACACTGCC 
AGAGGATTTA GTGGGACAGA GGAAAACCTG TATCAGCGCC AGCCAGCCAA ACTGAGTCCA 
ATCCTGGAGC AGAGTCCTTC TGATGAGAAG CCGTCTGAAG TTCCTGAGTG CAGTCTGAGG 
GCTCAGGGCA CCATCGTAGG AGAGGGAGTG AGTCTTCCTC AGCAGAACCA GACGCTGACC 
CACGACCAGA CCAGCTGCAG CATCGGCAGG CAGACGCTGG TCCCCCTGGC TTTACATGAT 
CACACTGCCG AGGCCAGCAG AGCCCTCAAC TCCAGCACTA AACCTAGCTG GAGTGTCTAC 
CAGAGCCCAG AGAGAACCAG CCAGACAGGT TCTAACACAG AACCTGTGTC CAACAGCATG 
CTGTCTGTGA AACAGCAAAT AGCTCACCAG CTCAATTCAT CTAATCTACA GCTTAATTCC 
AGCTCTAGCA TCAAACCCAG CTGGAGCATC TACCAGAGCC CAGAGAAAAC CAGCCATACG 
GGCTCGAACA GAGGGCTCAA CTCAAGCAGC ATGCTGTCTG TAAGAGAGCC TCAACCGATC 
CAATCCAATG CAAATGGCCT ACAGATGAAC TCTCTATCTT GCTCCCTTGC TGAGGCCAGC 
AATGCCCTCA ACTCCAGTGC CAAACCTAAC TGGAGCATCT ACCAGAGCCC AGAGAAAACC 
AGCCAGACAG ATTGTAACAC AGAACCGACC TCTAACAGCA TACTGTCGGT AAAGCAGTAC 
CAAGCAGACC AGTTCAATTT ACCTAGCATG CAGGCTAACT CCAGCTCCAG CACTAAACCC 
AGCTGGACCA TCTACCAAAG CCCAGAGAAA TCCCACCAGG CTAGTTTTTG TGCGGAACCC 
AAACAACACT CAAACAGCAT GCACTTGGCA GTGGGAGAAC CCAAACAGGA CCAGTTCAGC 
TCAACTGGCT TGCAGGTTAA TCCAGACAGA ACCATGAACT CCACCACCCA AAGCCCAGAG 
AGAACCCAAA AGACTCAATT TAGTGGGGAC CTCCACTCAA CCAGCATGCA TTTAGTGGCA 
GAACCCAAAC AAGACCAGCT TAACTCATCT GCCATTCAGC TGAACTCCAG CTGCAGCACC 
AAACCCAGCT GGAGTATTTA CCAGAGCCCA GAGAGAACCA GTCAAATGGG TCACAGCAGG 
GAGTTCAAGG CAGACCAGCA GGTTAACTCA AACCAAGGGT TCAACTCTTC TAGCCTGCAT 
TTAATGGGAG AGCTAAAACC AGACCAACTC AATTCCTCCA TCAATATGTT TTCTGGTCAG 
GGTGAGGCTG ATATTCTCTT GCCCAAGAAG TCTTTTCACC GGCGAAAATC CGAAAAACAG 
CTGAACTTCG AGAGCCTTCA GGTGAGCCGA CCTGTATCAC ACCAGTCCTG CACGCTGCCA 
GAGAGCCCAA AACCACAGCC CAGAATAATC CAGGATGTTC CCATGAGTCC AGACCCAATC 
CCAGGGTCCG ACTGGCTTCA GATGGACAGT CCAGTCCCGC TCTCTGAACC CAACCTGGAT 
GTGATGGTCA GGTCAATGAA CAGATCCCAG ACAATTCACA GAGAAACCCC CGGAGCTCCT 
GTTTCCAGGT CCAAACTCAG CTGGAGCGTG AACCAGACCC CAGGGAGGCC ATCTGAACTA 
CAGTACTTCC TGCAGAGTCC TGAGCCCATG GAGGAATCAG ATCCGCATCA GTTATCAAAG 
CTGGAAGAGT CTGGACTTCT GCTCTCCAAG AGATCCTTTA ACAGGCGCAA ATCGGATAAG 
AGGGTATTGG AGAAATCCCT CTTGTCGAGT CAGAGGCAGT CCTCCAACCA AAACCAGGAT 
GTTCCAATGA GTCCGGAACC AATTTCGGGG CTGAACTGGC TCTGTCCAGA GAGTCCAGCC 
CGTGTGACTG AGTCTGATCT GGACGTCATG ATGACACCTC AGCAAAAATC TAAATCAGTG 
GATGCTTCTG CCCAGCTGCT GCAGTGTGAT GTTCCAATGA GCCCCCTGCA GGCTTCCATA 
CCTGATTACA CTCCCTCAGT GTTGGTGTCT GACCCGTGGG ATGAGGACCT GATAGATTCT 
TTACTCTCCA GCCTGAAGAC GCCCCTCACC TCATACCCGA ACCTCAGCCT CTGCACTCGA 
GGCCTGCCCA CACTCACCCC CAAGATGTCC GTACAGATGG GTGAGGACTC TCTGCGGGTG 
GACTTTGTGT TGGGACAGGG TGCTTTCGCC ACCGTTTATC AGGCTACCAA CCTTACAACC 
TCACAAAAAT TCATCCTAAA GGTGCAGAAG CCGGCGAACC CATGGGAGTT TTACATCAAC 
AGCCAGCTAA ATGCTCGAGT GCTGCGGAAA GAGCGTCACC TGTTCAACCA GCTCCACTCC 
GCTCACCTGT TCACCAATGG CAGTGTGCTT GTGGGGGAAC TGCACAACTG CGGGACTCTG 
CTGAGTGTGG TGAATCTGTA TAAGAGCCGC AGTGAGAAGG TGATGCCCCA GCCGCTGGTG 
CTGTACTTCA GCATGTGTAT CCTGCAGATG GTGGAGCAGC TGCATCAAGC CAAAATCATC 
CACGCCGACA TCAAACCTGA CAACTTCCTG CTTGGAGAAA GGTTTCTGGA GAACGAGGAT 
TTGGATGCTG AGAGTCTGGA TCACGGCTTG GTTCTGATAG ATTTTGGTCA AAGTATTGAT 
ATGACCCTGT TCCCTGAGGG CACGGCCTTC ACTGCCCGCT GCATGACTTC TGGCTTCCAG 
TGCACAGAGA TGCTGAGTGG CCGGCCGTGG AACTACCAGA CTGATTACTT TGGCATTGCC 
GGCACCGTGC ACTGCATGAT CTTCGGGTCA TACATGCAAG TGAGAAATGA GGGTGGAGTT 
TGGAAAACGA ATGGCATTTT TAAAAGGAAT CCTCACAGTG ATCTATGGCA GGAGTTTTTC 
CATGTGCTGC TGAATGTTCC GGACTGTAAC TCTGCCTTAT GTTTGCAGAG TTTGCGTGAG 
CGTCTGTCTG TGGTGCTGCA GGAAAACTAC AGCAACAAAC TGCGCGGCCT TAAAAACCGA 
CTGGTGGTGC AGATCCTGGA GAGCCGCGGG GCTCGCAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007259947.2
CDS	505..5466
Translation	MDINSYLQQFEATVSSYVGEDPLDPWDKFVEFLECRLAAEERSNLCVVLERLVQTFLQDKRYRNDPRYINHCIKCAGYYGEPIKLYSYVHSLGVGARAAGLYVAWAQQFESKGLFTQAEQVYQRAIENQAEPMDTIQEQYRLFQSRMGKSQAASDHVRNPLQNSQLMNQNPRHRETLQPHCKEPDNSPLPADRTIRIISRSENNPMPRSSSSVESVPMYCVKELECEGSELSFEELRARRYFSKRQQLELLRKQEEVIWKHEEEEEEVIRMKKLLEELNSKLSTEQQETKHTFLQQELNESCSPLDRSVRQPLTPAMAEAAAPTSVTKEQAACSDIHRLQHAPERPLLNGRKSESHLLWQSTRVPASRLSLSLSAASQLAHSSALSAAVNRPLDLDTQAPVPLQLQDQRTASVCESQAERSLLESCSQRLAVFERDRSEKTEMDGGDANADTSHGGFNHSHVTPNTSLGLISATPSRALPSPTVNTREALDVIMDMFQAPTLLQDSVFNTTSQVADDSFEKNCRVYGFAPSVANPPSSMSFRIYQDENVKIGSEEQQVEKSKPAARALMEIPVSKPNVTPVGPESLTDESAMWGPQYASITACPNHTRDFAMAAHLVSTPLHPTAPYTWDTELNQVENTARGFSGTEENLYQRQPAKLSPILEQSPSDEKPSEVPECSLRAQGTIVGEGVSLPQQNQTLTHDQTSCSIGRQTLVPLALHDHTAEASRALNSSTKPSWSVYQSPERTSQTGSNTEPVSNSMLSVKQQIAHQLNSSNLQLNSSSSIKPSWSIYQSPEKTSHTGSNRGLNSSSMLSVREPQPIQSNANGLQMNSLSCSLAEASNALNSSAKPNWSIYQSPEKTSQTDCNTEPTSNSILSVKQYQADQFNLPSMQANSSSSTKPSWTIYQSPEKSHQASFCAEPKQHSNSMHLAVGEPKQDQFSSTGLQVNPDRTMNSTTQSPERTQKTQFSGDLHSTSMHLVAEPKQDQLNSSAIQLNSSCSTKPSWSIYQSPERTSQMGHSREFKADQQVNSNQGFNSSSLHLMGELKPDQLNSSINMFSGQGEADILLPKKSFHRRKSEKQLNFESLQVSRPVSHQSCTLPESPKPQPRIIQDVPMSPDPIPGSDWLQMDSPVPLSEPNLDVMVRSMNRSQTIHRETPGAPVSRSKLSWSVNQTPGRPSELQYFLQSPEPMEESDPHQLSKLEESGLLLSKRSFNRRKSDKRVLEKSLLSSQRQSSNQNQDVPMSPEPISGLNWLCPESPARVTESDLDVMMTPQQKSKSVDASAQLLQCDVPMSPLQASIPDYTPSVLVSDPWDEDLIDSLLSSLKTPLTSYPNLSLCTRGLPTLTPKMSVQMGEDSLRVDFVLGQGAFATVYQATNLTTSQKFILKVQKPANPWEFYINSQLNARVLRKERHLFNQLHSAHLFTNGSVLVGELHNCGTLLSVVNLYKSRSEKVMPQPLVLYFSMCILQMVEQLHQAKIIHADIKPDNFLLGERFLENEDLDAESLDHGLVLIDFGQSIDMTLFPEGTAFTARCMTSGFQCTEMLSGRPWNYQTDYFGIAGTVHCMIFGSYMQVRNEGGVWKTNGIFKRNPHSDLWQEFFHVLLNVPDCNSALCLQSLRERLSVVLQENYSNKLRGLKNRLVVQILESRGARR

Target ORF information:

RefSeq Version	XM_007259885.3
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007259885.3

ORF Insert Sequence:

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CAACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCCTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGCGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACACATCTC ACGGAGGCTT TAATCATTCT 
CATGTCACTC CTAACACATC TCTGGGTCTG ATTTCGGCGA CTCCTTCACG TGCACTCCCC 
TCTCCCACTG TCAACACTCG GGAGGCTCTG GACGTGATCA TGGACATGTT CCAGGCTCCA 
ACGCTGCTGC AGGACTCCGT CTTCAATACT ACCAGCCAGG TGGCTGATGA CAGCTTTGAG 
AAAAACTGCA GAGTATACGG TTTTGCCCCA TCTGTAGCTA ATCCACCCTC CTCAATGTCG 
TTCAGGATAT ACCAGGATGA AAATGTGAAG ATTGGAAGTG AGGAACAACA GGTGGAGAAG 
AGCAAACCAG CAGCTCGAGC CCTGATGGAG ATTCCGGTCT CCAAACCCAA TGTCACTCCT 
GTAGGTCCGG AGTCTCTGAC AGACGAGAGT GCGATGTGGG GTCCTCAATA TGCCTCCATA 
ACTGCCTGTC CAAATCATAC TCGTGATTTT GCCATGGCTG CCCACCTGGT ATCCACCCCC 
CTCCACCCCA CTGCCCCGTA CACCTGGGAC ACAGAGCTCA ATCAGGTAGA AAACACTGCC 
AGAGGATTTA GTGGGACAGA GGAAAACCTG TATCAGCGCC AGCCAGCCAA ACTGAGTCCA 
ATCCTGGAGC AGAGTCCTTC TGATGAGAAG CCGTCTGAAG TTCCTGAGTG CAGTCTGAGG 
GCTCAGGGCA CCATCGTAGG AGAGGGAGTG AGTCTTCCTC AGCAGAACCA GACGCTGACC 
CACGACCAGA CCAGCTGCAG CATCGGCAGG CAGACGCTGG TCCCCCTGGC TTTACATGAT 
CACACTGCCG AGGCCAGCAG AGCCCTCAAC TCCAGCACTA AACCTAGCTG GAGTGTCTAC 
CAGAGCCCAG AGAGAACCAG CCAGACAGGT TCTAACACAG AACCTGTGTC CAACAGCATG 
CTGTCTGTGA AACAGCAAAT AGCTCACCAG CTCAATTCAT CTAATCTACA GCTTAATTCC 
AGCTCTAGCA TCAAACCCAG CTGGAGCATC TACCAGAGCC CAGAGAAAAC CAGCCATACG 
GGCTCGAACA GAGGGCTCAA CTCAAGCAGC ATGCTGTCTG TAAGAGAGCC TCAACCGATC 
CAATCCAATG CAAATGGCCT ACAGATGAAC TCTCTATCTT GCTCCCTTGC TGAGGCCAGC 
AATGCCCTCA ACTCCAGTGC CAAACCTAAC TGGAGCATCT ACCAGAGCCC AGAGAAAACC 
AGCCAGACAG ATTGTAACAC AGAACCGACC TCTAACAGCA TACTGTCGGT AAAGCAGTAC 
CAAGCAGACC AGTTCAATTT ACCTAGCATG CAGGCTAACT CCAGCTCCAG CACTAAACCC 
AGCTGGACCA TCTACCAAAG CCCAGAGAAA TCCCACCAGG CTAGTTTTTG TGCGGAACCC 
AAACAACACT CAAACAGCAT GCACTTGGCA GTGGGAGAAC CCAAACAGGA CCAGTTCAGC 
TCAACTGGCT TGCAGGTTAA TCCAGACAGA ACCATGAACT CCACCACCCA AAGCCCAGAG 
AGAACCCAAA AGACTCAATT TAGTGGGGAC CTCCACTCAA CCAGCATGCA TTTAGTGGCA 
GAACCCAAAC AAGACCAGCT TAACTCATCT GCCATTCAGC TGAACTCCAG CTGCAGCACC 
AAACCCAGCT GGAGTATTTA CCAGAGCCCA GAGAGAACCA GTCAAATGGG TCACAGCAGG 
GAGTTCAAGG CAGACCAGCA GGTTAACTCA AACCAAGGGT TCAACTCTTC TAGCCTGCAT 
TTAATGGGAG AGCTAAAACC AGACCAACTC AATTCCTCCA TCAATATGTT TTCTGGTCAG 
GGTGAGGCTG ATATTCTCTT GCCCAAGAAG TCTTTTCACC GGCGAAAATC CGAAAAACAG 
CTGAACTTCG AGAGCCTTCA GGTGAGCCGA CCTGTATCAC ACCAGTCCTG CACGCTGCCA 
GAGAGCCCAA AACCACAGCC CAGAATAATC CAGGATGTTC CCATGAGTCC AGACCCAATC 
CCAGGGTCCG ACTGGCTTCA GATGGACAGT CCAGTCCCGC TCTCTGAACC CAACCTGGAT 
GTGATGGTCA GGTCAATGAA CAGATCCCAG ACAATTCACA GAGAAACCCC CGGAGCTCCT 
GTTTCCAGGT CCAAACTCAG CTGGAGCGTG AACCAGACCC CAGGGAGGCC ATCTGAACTA 
CAGTACTTCC TGCAGAGTCC TGAGCCCATG GAGGAATCAG ATCCGCATCA GTTATCAAAG 
CTGGAAGAGT CTGGACTTCT GCTCTCCAAG AGATCCTTTA ACAGGCGCAA ATCGGATAAG 
AGGGTATTGG AGAAATCCCT CTTGTCGAGT CAGAGGCAGT CCTCCAACCA AAACCAGGAT 
GTTCCAATGA GTCCGGAACC AATTTCGGGG CTGAACTGGC TCTGTCCAGA GAGTCCAGCC 
CGTGTGACTG AGTCTGATCT GGACGTCATG ATGACACCTC AGCAAAAATC TAAATCAGTG 
GATGCTTCTG CCCAGCTGCT GCAGTGTGAT GTTCCAATGA GCCCCCTGCA GGCTTCCATA 
CCTGATTACA CTCCCTCAGT GTTGGTGTCT GACCCGTGGG ATGAGGACCT GATAGATTCT 
TTACTCTCCA GCCTGAAGAC GCCCCTCACC TCATACCCGA ACCTCAGCCT CTGCACTCGA 
GGCCTGCCCA CACTCACCCC CAAGATGTCC GTACAGATGG GTGAGGACTC TCTGCGGGTG 
GACTTTGTGT TGGGACAGGG TGCTTTCGCC ACCGTTTATC AGGCTACCAA CCTTACAACC 
TCACAAAAAT TCATCCTAAA GGTGCAGAAG CCGGCGAACC CATGGGAGTT TTACATCAAC 
AGCCAGCTAA ATGCTCGAGT GCTGCGGAAA GAGCGTCACC TGTTCAACCA GCTCCACTCC 
GCTCACCTGT TCACCAATGG CAGTGTGCTT GTGGGGGAAC TGCACAACTG CGGGACTCTG 
CTGAGTGTGG TGAATCTGTA TAAGAGCCGC AGTGAGAAGG TGATGCCCCA GCCGCTGGTG 
CTGTACTTCA GCATGTGTAT CCTGCAGATG GTGGAGCAGC TGCATCAAGC CAAAATCATC 
CACGCCGACA TCAAACCTGA CAACTTCCTG CTTGGAGAAA GGTTTCTGGA GAACGAGGAT 
TTGGATGCTG AGAGTCTGGA TCACGGCTTG GTTCTGATAG ATTTTGGTCA AAGTATTGAT 
ATGACCCTGT TCCCTGAGGG CACGGCCTTC ACTGCCCGCT GCATGACTTC TGGCTTCCAG 
TGCACAGAGA TGCTGAGTGG CCGGCCGTGG AACTACCAGA CTGATTACTT TGGCATTGCC 
GGCACCGTGC ACTGCATGAT CTTCGGGTCA TACATGCAAG TGAGAAATGA GGGTGGAGTT 
TGGAAAACGA ATGGCATTTT TAAAAGGAAT CCTCACAGTG ATCTATGGCA GGAGTTTTTC 
CATGTGCTGC TGAATGTTCC GGACTGTAAC TCTGCCTTAT GTTTGCAGAG TTTGCGTGAG 
CGTCTGTCTG TGGTGCTGCA GGAAAACTAC AGCAACAAAC TGCGCGGCCT TAAAAACCGA 
CTGGTGGTGC AGATCCTGGA GAGCCGCGGG GCTCGCAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_015608507.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015463993.2 Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAs123417
Clone ID Related Accession (Same CDS sequence)	XM_015608507.2
Accession Version	XM_015608507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_015608507.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CAACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCCTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGCGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACACATCTC ACGGAGGCTT TAATCATTCT 
CATGTCACTC CTAACACATC TCTGGGTCTG ATTTCGGCGA CTCCTTCACG TGCACTCCCC 
TCTCCCACTG TCAACACTCG GGAGGCTCTG GACGTGATCA TGGACATGTT CCAGGCTCCA 
ACGCTGCTGC AGGACTCCGT CTTCAATACT ACCAGCCAGG TGGCTGATGA CAGCTTTGAG 
AAAAACTGCA GAGTATACGG TTTTGCCCCA TCTGTAGCTA ATCCACCCTC CTCAATGTCG 
TTCAGGATAT ACCAGGATGA AAATGTGAAG ATTGGAAGTG AGGAACAACA GGTGGAGAAG 
AGCAAACCAG CAGCTCGAGC CCTGATGGAG ATTCCGGTCT CCAAACCCAA TGTCACTCCT 
GTAGGTCCGG AGTCTCTGAC AGACGAGAGT GCGATGTGGG GTCCTCAATA TGCCTCCATA 
ACTGCCTGTC CAAATCATAC TCGTGATTTT GCCATGGCTG CCCACCTGGT ATCCACCCCC 
CTCCACCCCA CTGCCCCGTA CACCTGGGAC ACAGAGCTCA ATCAGGAAAA CACTGCCAGA 
GGATTTAGTG GGACAGAGGA AAACCTGTAT CAGCGCCAGC CAGCCAAACT GAGTCCAATC 
CTGGAGCAGA GTCCTTCTGA TGAGAAGCCG TCTGAAGTTC CTGAGTGCAG TCTGAGGGCT 
CAGGGCACCA TCGTAGGAGA GGGAGTGAGT CTTCCTCAGC AGAACCAGAC GCTGACCCAC 
GACCAGACCA GCTGCAGCAT CGGCAGGCAG ACGCTGGTCC CCCTGGCTTT ACATGATCAC 
ACTGCCGAGG CCAGCAGAGC CCTCAACTCC AGCACTAAAC CTAGCTGGAG TGTCTACCAG 
AGCCCAGAGA GAACCAGCCA GACAGGTTCT AACACAGAAC CTGTGTCCAA CAGCATGCTG 
TCTGTGAAAC AGCAAATAGC TCACCAGCTC AATTCATCTA ATCTACAGCT TAATTCCAGC 
TCTAGCATCA AACCCAGCTG GAGCATCTAC CAGAGCCCAG AGAAAACCAG CCATACGGGC 
TCGAACAGAG GGCTCAACTC AAGCAGCATG CTGTCTGTAA GAGAGCCTCA ACCGATCCAA 
TCCAATGCAA ATGGCCTACA GATGAACTCT CTATCTTGCT CCCTTGCTGA GGCCAGCAAT 
GCCCTCAACT CCAGTGCCAA ACCTAACTGG AGCATCTACC AGAGCCCAGA GAAAACCAGC 
CAGACAGATT GTAACACAGA ACCGACCTCT AACAGCATAC TGTCGGTAAA GCAGTACCAA 
GCAGACCAGT TCAATTTACC TAGCATGCAG GCTAACTCCA GCTCCAGCAC TAAACCCAGC 
TGGACCATCT ACCAAAGCCC AGAGAAATCC CACCAGGCTA GTTTTTGTGC GGAACCCAAA 
CAACACTCAA ACAGCATGCA CTTGGCAGTG GGAGAACCCA AACAGGACCA GTTCAGCTCA 
ACTGGCTTGC AGGTTAATCC AGACAGAACC ATGAACTCCA CCACCCAAAG CCCAGAGAGA 
ACCCAAAAGA CTCAATTTAG TGGGGACCTC CACTCAACCA GCATGCATTT AGTGGCAGAA 
CCCAAACAAG ACCAGCTTAA CTCATCTGCC ATTCAGCTGA ACTCCAGCTG CAGCACCAAA 
CCCAGCTGGA GTATTTACCA GAGCCCAGAG AGAACCAGTC AAATGGGTCA CAGCAGGGAG 
TTCAAGGCAG ACCAGCAGGT TAACTCAAAC CAAGGGTTCA ACTCTTCTAG CCTGCATTTA 
ATGGGAGAGC TAAAACCAGA CCAACTCAAT TCCTCCATCA ATATGTTTTC TGGTCAGGGT 
GAGGCTGATA TTCTCTTGCC CAAGAAGTCT TTTCACCGGC GAAAATCCGA AAAACAGCTG 
AACTTCGAGA GCCTTCAGGT GAGCCGACCT GTATCACACC AGTCCTGCAC GCTGCCAGAG 
AGCCCAAAAC CACAGCCCAG AATAATCCAG GATGTTCCCA TGAGTCCAGA CCCAATCCCA 
GGGTCCGACT GGCTTCAGAT GGACAGTCCA GTCCCGCTCT CTGAACCCAA CCTGGATGTG 
ATGGTCAGGT CAATGAACAG ATCCCAGACA ATTCACAGAG AAACCCCCGG AGCTCCTGTT 
TCCAGGTCCA AACTCAGCTG GAGCGTGAAC CAGACCCCAG GGAGGCCATC TGAACTACAG 
TACTTCCTGC AGAGTCCTGA GCCCATGGAG GAATCAGATC CGCATCAGTT ATCAAAGCTG 
GAAGAGTCTG GACTTCTGCT CTCCAAGAGA TCCTTTAACA GGCGCAAATC GGATAAGAGG 
GTATTGGAGA AATCCCTCTT GTCGAGTCAG AGGCAGTCCT CCAACCAAAA CCAGGATGTT 
CCAATGAGTC CGGAACCAAT TTCGGGGCTG AACTGGCTCT GTCCAGAGAG TCCAGCCCGT 
GTGACTGAGT CTGATCTGGA CGTCATGATG ACACCTCAGC AAAAATCTAA ATCAGTGGAT 
GCTTCTGCCC AGCTGCTGCA GTGTGATGTT CCAATGAGCC CCCTGCAGGC TTCCATACCT 
GATTACACTC CCTCAGTGTT GGTGTCTGAC CCGTGGGATG AGGACCTGAT AGATTCTTTA 
CTCTCCAGCC TGAAGACGCC CCTCACCTCA TACCCGAACC TCAGCCTCTG CACTCGAGGC 
CTGCCCACAC TCACCCCCAA GATGTCCGTA CAGATGGGTG AGGACTCTCT GCGGGTGGAC 
TTTGTGTTGG GACAGGGTGC TTTCGCCACC GTTTATCAGG CTACCAACCT TACAACCTCA 
CAAAAATTCA TCCTAAAGGT GCAGAAGCCG GCGAACCCAT GGGAGTTTTA CATCAACAGC 
CAGCTAAATG CTCGAGTGCT GCGGAAAGAG CGTCACCTGT TCAACCAGCT CCACTCCGCT 
CACCTGTTCA CCAATGGCAG TGTGCTTGTG GGGGAACTGC ACAACTGCGG GACTCTGCTG 
AGTGTGGTGA ATCTGTATAA GAGCCGCAGT GAGAAGGTGA TGCCCCAGCC GCTGGTGCTG 
TACTTCAGCA TGTGTATCCT GCAGATGGTG GAGCAGCTGC ATCAAGCCAA AATCATCCAC 
GCCGACATCA AACCTGACAA CTTCCTGCTT GGAGAAAGGT TTCTGGAGAA CGAGGATTTG 
GATGCTGAGA GTCTGGATCA CGGCTTGGTT CTGATAGATT TTGGTCAAAG TATTGATATG 
ACCCTGTTCC CTGAGGGCAC GGCCTTCACT GCCCGCTGCA TGACTTCTGG CTTCCAGTGC 
ACAGAGATGC TGAGTGGCCG GCCGTGGAAC TACCAGACTG ATTACTTTGG CATTGCCGGC 
ACCGTGCACT GCATGATCTT CGGGTCATAC ATGCAAGTGA GAAATGAGGG TGGAGTTTGG 
AAAACGAATG GCATTTTTAA AAGGAATCCT CACAGTGATC TATGGCAGGA GTTTTTCCAT 
GTGCTGCTGA ATGTTCCGGA CTGTAACTCT GCCTTATGTT TGCAGAGTTT GCGTGAGCGT 
CTGTCTGTGG TGCTGCAGGA AAACTACAGC AACAAACTGC GCGGCCTTAA AAACCGACTG 
GTGGTGCAGA TCCTGGAGAG CCGCGGGGCT CGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015463993.2
CDS	506..5464
Translation	MDINSYLQQFEATVSSYVGEDPLDPWDKFVEFLECRLAAEERSNLCVVLERLVQTFLQDKRYRNDPRYINHCIKCAGYYGEPIKLYSYVHSLGVGARAAGLYVAWAQQFESKGLFTQAEQVYQRAIENQAEPMDTIQEQYRLFQSRMGKSQAASDHVRNPLQNSQLMNQNPRHRETLQPHCKEPDNSPLPADRTIRIISRSENNPMPRSSSSVESVPMYCVKELECEGSELSFEELRARRYFSKRQQLELLRKQEEVIWKHEEEEEEVIRMKKLLEELNSKLSTEQQETKHTFLQQELNESCSPLDRSVRQPLTPAMAEAAAPTSVTKEQAACSDIHRLQHAPERPLLNGRKSESHLLWQSTRVPASRLSLSLSAASQLAHSSALSAAVNRPLDLDTQAPVPLQLQDQRTASVCESQAERSLLESCSQRLAVFERDRSEKTEMDGGDANADTSHGGFNHSHVTPNTSLGLISATPSRALPSPTVNTREALDVIMDMFQAPTLLQDSVFNTTSQVADDSFEKNCRVYGFAPSVANPPSSMSFRIYQDENVKIGSEEQQVEKSKPAARALMEIPVSKPNVTPVGPESLTDESAMWGPQYASITACPNHTRDFAMAAHLVSTPLHPTAPYTWDTELNQENTARGFSGTEENLYQRQPAKLSPILEQSPSDEKPSEVPECSLRAQGTIVGEGVSLPQQNQTLTHDQTSCSIGRQTLVPLALHDHTAEASRALNSSTKPSWSVYQSPERTSQTGSNTEPVSNSMLSVKQQIAHQLNSSNLQLNSSSSIKPSWSIYQSPEKTSHTGSNRGLNSSSMLSVREPQPIQSNANGLQMNSLSCSLAEASNALNSSAKPNWSIYQSPEKTSQTDCNTEPTSNSILSVKQYQADQFNLPSMQANSSSSTKPSWTIYQSPEKSHQASFCAEPKQHSNSMHLAVGEPKQDQFSSTGLQVNPDRTMNSTTQSPERTQKTQFSGDLHSTSMHLVAEPKQDQLNSSAIQLNSSCSTKPSWSIYQSPERTSQMGHSREFKADQQVNSNQGFNSSSLHLMGELKPDQLNSSINMFSGQGEADILLPKKSFHRRKSEKQLNFESLQVSRPVSHQSCTLPESPKPQPRIIQDVPMSPDPIPGSDWLQMDSPVPLSEPNLDVMVRSMNRSQTIHRETPGAPVSRSKLSWSVNQTPGRPSELQYFLQSPEPMEESDPHQLSKLEESGLLLSKRSFNRRKSDKRVLEKSLLSSQRQSSNQNQDVPMSPEPISGLNWLCPESPARVTESDLDVMMTPQQKSKSVDASAQLLQCDVPMSPLQASIPDYTPSVLVSDPWDEDLIDSLLSSLKTPLTSYPNLSLCTRGLPTLTPKMSVQMGEDSLRVDFVLGQGAFATVYQATNLTTSQKFILKVQKPANPWEFYINSQLNARVLRKERHLFNQLHSAHLFTNGSVLVGELHNCGTLLSVVNLYKSRSEKVMPQPLVLYFSMCILQMVEQLHQAKIIHADIKPDNFLLGERFLENEDLDAESLDHGLVLIDFGQSIDMTLFPEGTAFTARCMTSGFQCTEMLSGRPWNYQTDYFGIAGTVHCMIFGSYMQVRNEGGVWKTNGIFKRNPHSDLWQEFFHVLLNVPDCNSALCLQSLRERLSVVLQENYSNKLRGLKNRLVVQILESRGARR

Target ORF information:

RefSeq Version	XM_015608507.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015608507.2

ORF Insert Sequence:

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CAACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCCTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGCGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACACATCTC ACGGAGGCTT TAATCATTCT 
CATGTCACTC CTAACACATC TCTGGGTCTG ATTTCGGCGA CTCCTTCACG TGCACTCCCC 
TCTCCCACTG TCAACACTCG GGAGGCTCTG GACGTGATCA TGGACATGTT CCAGGCTCCA 
ACGCTGCTGC AGGACTCCGT CTTCAATACT ACCAGCCAGG TGGCTGATGA CAGCTTTGAG 
AAAAACTGCA GAGTATACGG TTTTGCCCCA TCTGTAGCTA ATCCACCCTC CTCAATGTCG 
TTCAGGATAT ACCAGGATGA AAATGTGAAG ATTGGAAGTG AGGAACAACA GGTGGAGAAG 
AGCAAACCAG CAGCTCGAGC CCTGATGGAG ATTCCGGTCT CCAAACCCAA TGTCACTCCT 
GTAGGTCCGG AGTCTCTGAC AGACGAGAGT GCGATGTGGG GTCCTCAATA TGCCTCCATA 
ACTGCCTGTC CAAATCATAC TCGTGATTTT GCCATGGCTG CCCACCTGGT ATCCACCCCC 
CTCCACCCCA CTGCCCCGTA CACCTGGGAC ACAGAGCTCA ATCAGGAAAA CACTGCCAGA 
GGATTTAGTG GGACAGAGGA AAACCTGTAT CAGCGCCAGC CAGCCAAACT GAGTCCAATC 
CTGGAGCAGA GTCCTTCTGA TGAGAAGCCG TCTGAAGTTC CTGAGTGCAG TCTGAGGGCT 
CAGGGCACCA TCGTAGGAGA GGGAGTGAGT CTTCCTCAGC AGAACCAGAC GCTGACCCAC 
GACCAGACCA GCTGCAGCAT CGGCAGGCAG ACGCTGGTCC CCCTGGCTTT ACATGATCAC 
ACTGCCGAGG CCAGCAGAGC CCTCAACTCC AGCACTAAAC CTAGCTGGAG TGTCTACCAG 
AGCCCAGAGA GAACCAGCCA GACAGGTTCT AACACAGAAC CTGTGTCCAA CAGCATGCTG 
TCTGTGAAAC AGCAAATAGC TCACCAGCTC AATTCATCTA ATCTACAGCT TAATTCCAGC 
TCTAGCATCA AACCCAGCTG GAGCATCTAC CAGAGCCCAG AGAAAACCAG CCATACGGGC 
TCGAACAGAG GGCTCAACTC AAGCAGCATG CTGTCTGTAA GAGAGCCTCA ACCGATCCAA 
TCCAATGCAA ATGGCCTACA GATGAACTCT CTATCTTGCT CCCTTGCTGA GGCCAGCAAT 
GCCCTCAACT CCAGTGCCAA ACCTAACTGG AGCATCTACC AGAGCCCAGA GAAAACCAGC 
CAGACAGATT GTAACACAGA ACCGACCTCT AACAGCATAC TGTCGGTAAA GCAGTACCAA 
GCAGACCAGT TCAATTTACC TAGCATGCAG GCTAACTCCA GCTCCAGCAC TAAACCCAGC 
TGGACCATCT ACCAAAGCCC AGAGAAATCC CACCAGGCTA GTTTTTGTGC GGAACCCAAA 
CAACACTCAA ACAGCATGCA CTTGGCAGTG GGAGAACCCA AACAGGACCA GTTCAGCTCA 
ACTGGCTTGC AGGTTAATCC AGACAGAACC ATGAACTCCA CCACCCAAAG CCCAGAGAGA 
ACCCAAAAGA CTCAATTTAG TGGGGACCTC CACTCAACCA GCATGCATTT AGTGGCAGAA 
CCCAAACAAG ACCAGCTTAA CTCATCTGCC ATTCAGCTGA ACTCCAGCTG CAGCACCAAA 
CCCAGCTGGA GTATTTACCA GAGCCCAGAG AGAACCAGTC AAATGGGTCA CAGCAGGGAG 
TTCAAGGCAG ACCAGCAGGT TAACTCAAAC CAAGGGTTCA ACTCTTCTAG CCTGCATTTA 
ATGGGAGAGC TAAAACCAGA CCAACTCAAT TCCTCCATCA ATATGTTTTC TGGTCAGGGT 
GAGGCTGATA TTCTCTTGCC CAAGAAGTCT TTTCACCGGC GAAAATCCGA AAAACAGCTG 
AACTTCGAGA GCCTTCAGGT GAGCCGACCT GTATCACACC AGTCCTGCAC GCTGCCAGAG 
AGCCCAAAAC CACAGCCCAG AATAATCCAG GATGTTCCCA TGAGTCCAGA CCCAATCCCA 
GGGTCCGACT GGCTTCAGAT GGACAGTCCA GTCCCGCTCT CTGAACCCAA CCTGGATGTG 
ATGGTCAGGT CAATGAACAG ATCCCAGACA ATTCACAGAG AAACCCCCGG AGCTCCTGTT 
TCCAGGTCCA AACTCAGCTG GAGCGTGAAC CAGACCCCAG GGAGGCCATC TGAACTACAG 
TACTTCCTGC AGAGTCCTGA GCCCATGGAG GAATCAGATC CGCATCAGTT ATCAAAGCTG 
GAAGAGTCTG GACTTCTGCT CTCCAAGAGA TCCTTTAACA GGCGCAAATC GGATAAGAGG 
GTATTGGAGA AATCCCTCTT GTCGAGTCAG AGGCAGTCCT CCAACCAAAA CCAGGATGTT 
CCAATGAGTC CGGAACCAAT TTCGGGGCTG AACTGGCTCT GTCCAGAGAG TCCAGCCCGT 
GTGACTGAGT CTGATCTGGA CGTCATGATG ACACCTCAGC AAAAATCTAA ATCAGTGGAT 
GCTTCTGCCC AGCTGCTGCA GTGTGATGTT CCAATGAGCC CCCTGCAGGC TTCCATACCT 
GATTACACTC CCTCAGTGTT GGTGTCTGAC CCGTGGGATG AGGACCTGAT AGATTCTTTA 
CTCTCCAGCC TGAAGACGCC CCTCACCTCA TACCCGAACC TCAGCCTCTG CACTCGAGGC 
CTGCCCACAC TCACCCCCAA GATGTCCGTA CAGATGGGTG AGGACTCTCT GCGGGTGGAC 
TTTGTGTTGG GACAGGGTGC TTTCGCCACC GTTTATCAGG CTACCAACCT TACAACCTCA 
CAAAAATTCA TCCTAAAGGT GCAGAAGCCG GCGAACCCAT GGGAGTTTTA CATCAACAGC 
CAGCTAAATG CTCGAGTGCT GCGGAAAGAG CGTCACCTGT TCAACCAGCT CCACTCCGCT 
CACCTGTTCA CCAATGGCAG TGTGCTTGTG GGGGAACTGC ACAACTGCGG GACTCTGCTG 
AGTGTGGTGA ATCTGTATAA GAGCCGCAGT GAGAAGGTGA TGCCCCAGCC GCTGGTGCTG 
TACTTCAGCA TGTGTATCCT GCAGATGGTG GAGCAGCTGC ATCAAGCCAA AATCATCCAC 
GCCGACATCA AACCTGACAA CTTCCTGCTT GGAGAAAGGT TTCTGGAGAA CGAGGATTTG 
GATGCTGAGA GTCTGGATCA CGGCTTGGTT CTGATAGATT TTGGTCAAAG TATTGATATG 
ACCCTGTTCC CTGAGGGCAC GGCCTTCACT GCCCGCTGCA TGACTTCTGG CTTCCAGTGC 
ACAGAGATGC TGAGTGGCCG GCCGTGGAAC TACCAGACTG ATTACTTTGG CATTGCCGGC 
ACCGTGCACT GCATGATCTT CGGGTCATAC ATGCAAGTGA GAAATGAGGG TGGAGTTTGG 
AAAACGAATG GCATTTTTAA AAGGAATCCT CACAGTGATC TATGGCAGGA GTTTTTCCAT 
GTGCTGCTGA ATGTTCCGGA CTGTAACTCT GCCTTATGTT TGCAGAGTTT GCGTGAGCGT 
CTGTCTGTGG TGCTGCAGGA AAACTACAGC AACAAACTGC GCGGCCTTAA AAACCGACTG 
GTGGTGCAGA TCCTGGAGAG CCGCGGGGCT CGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_007259886.3 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007259948.2 Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.

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CloneID	OAs123418
Clone ID Related Accession (Same CDS sequence)	XM_007259886.3
Accession Version	XM_007259886.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_007259886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CAACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCCTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGCGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACAACGTGA TCATGGACAT GTTCCAGGCT 
CCAACGCTGC TGCAGGACTC CGTCTTCAAT ACTACCAGCC AGGTGGCTGA TGACAGCTTT 
GAGAAAAACT GCAGAGTATA CGGTTTTGCC CCATCTGTAG CTAATCCACC CTCCTCAATG 
TCGTTCAGGA TATACCAGGA TGAAAATGTG AAGATTGGAA GTGAGGAACA ACAGGTGGAG 
AAGAGCAAAC CAGCAGCTCG AGCCCTGATG GAGATTCCGG TCTCCAAACC CAATGTCACT 
CCTGTAGGTC CGGAGTCTCT GACAGACGAG AGTGCGATGT GGGGTCCTCA ATATGCCTCC 
ATAACTGCCT GTCCAAATCA TACTCGTGAT TTTGCCATGG CTGCCCACCT GGTATCCACC 
CCCCTCCACC CCACTGCCCC GTACACCTGG GACACAGAGC TCAATCAGGT AGAAAACACT 
GCCAGAGGAT TTAGTGGGAC AGAGGAAAAC CTGTATCAGC GCCAGCCAGC CAAACTGAGT 
CCAATCCTGG AGCAGAGTCC TTCTGATGAG AAGCCGTCTG AAGTTCCTGA GTGCAGTCTG 
AGGGCTCAGG GCACCATCGT AGGAGAGGGA GTGAGTCTTC CTCAGCAGAA CCAGACGCTG 
ACCCACGACC AGACCAGCTG CAGCATCGGC AGGCAGACGC TGGTCCCCCT GGCTTTACAT 
GATCACACTG CCGAGGCCAG CAGAGCCCTC AACTCCAGCA CTAAACCTAG CTGGAGTGTC 
TACCAGAGCC CAGAGAGAAC CAGCCAGACA GGTTCTAACA CAGAACCTGT GTCCAACAGC 
ATGCTGTCTG TGAAACAGCA AATAGCTCAC CAGCTCAATT CATCTAATCT ACAGCTTAAT 
TCCAGCTCTA GCATCAAACC CAGCTGGAGC ATCTACCAGA GCCCAGAGAA AACCAGCCAT 
ACGGGCTCGA ACAGAGGGCT CAACTCAAGC AGCATGCTGT CTGTAAGAGA GCCTCAACCG 
ATCCAATCCA ATGCAAATGG CCTACAGATG AACTCTCTAT CTTGCTCCCT TGCTGAGGCC 
AGCAATGCCC TCAACTCCAG TGCCAAACCT AACTGGAGCA TCTACCAGAG CCCAGAGAAA 
ACCAGCCAGA CAGATTGTAA CACAGAACCG ACCTCTAACA GCATACTGTC GGTAAAGCAG 
TACCAAGCAG ACCAGTTCAA TTTACCTAGC ATGCAGGCTA ACTCCAGCTC CAGCACTAAA 
CCCAGCTGGA CCATCTACCA AAGCCCAGAG AAATCCCACC AGGCTAGTTT TTGTGCGGAA 
CCCAAACAAC ACTCAAACAG CATGCACTTG GCAGTGGGAG AACCCAAACA GGACCAGTTC 
AGCTCAACTG GCTTGCAGGT TAATCCAGAC AGAACCATGA ACTCCACCAC CCAAAGCCCA 
GAGAGAACCC AAAAGACTCA ATTTAGTGGG GACCTCCACT CAACCAGCAT GCATTTAGTG 
GCAGAACCCA AACAAGACCA GCTTAACTCA TCTGCCATTC AGCTGAACTC CAGCTGCAGC 
ACCAAACCCA GCTGGAGTAT TTACCAGAGC CCAGAGAGAA CCAGTCAAAT GGGTCACAGC 
AGGGAGTTCA AGGCAGACCA GCAGGTTAAC TCAAACCAAG GGTTCAACTC TTCTAGCCTG 
CATTTAATGG GAGAGCTAAA ACCAGACCAA CTCAATTCCT CCATCAATAT GTTTTCTGGT 
CAGGGTGAGG CTGATATTCT CTTGCCCAAG AAGTCTTTTC ACCGGCGAAA ATCCGAAAAA 
CAGCTGAACT TCGAGAGCCT TCAGGTGAGC CGACCTGTAT CACACCAGTC CTGCACGCTG 
CCAGAGAGCC CAAAACCACA GCCCAGAATA ATCCAGGATG TTCCCATGAG TCCAGACCCA 
ATCCCAGGGT CCGACTGGCT TCAGATGGAC AGTCCAGTCC CGCTCTCTGA ACCCAACCTG 
GATGTGATGG TCAGGTCAAT GAACAGATCC CAGACAATTC ACAGAGAAAC CCCCGGAGCT 
CCTGTTTCCA GGTCCAAACT CAGCTGGAGC GTGAACCAGA CCCCAGGGAG GCCATCTGAA 
CTACAGTACT TCCTGCAGAG TCCTGAGCCC ATGGAGGAAT CAGATCCGCA TCAGTTATCA 
AAGCTGGAAG AGTCTGGACT TCTGCTCTCC AAGAGATCCT TTAACAGGCG CAAATCGGAT 
AAGAGGGTAT TGGAGAAATC CCTCTTGTCG AGTCAGAGGC AGTCCTCCAA CCAAAACCAG 
GATGTTCCAA TGAGTCCGGA ACCAATTTCG GGGCTGAACT GGCTCTGTCC AGAGAGTCCA 
GCCCGTGTGA CTGAGTCTGA TCTGGACGTC ATGATGACAC CTCAGCAAAA ATCTAAATCA 
GTGGATGCTT CTGCCCAGCT GCTGCAGTGT GATGTTCCAA TGAGCCCCCT GCAGGCTTCC 
ATACCTGATT ACACTCCCTC AGTGTTGGTG TCTGACCCGT GGGATGAGGA CCTGATAGAT 
TCTTTACTCT CCAGCCTGAA GACGCCCCTC ACCTCATACC CGAACCTCAG CCTCTGCACT 
CGAGGCCTGC CCACACTCAC CCCCAAGATG TCCGTACAGA TGGGTGAGGA CTCTCTGCGG 
GTGGACTTTG TGTTGGGACA GGGTGCTTTC GCCACCGTTT ATCAGGCTAC CAACCTTACA 
ACCTCACAAA AATTCATCCT AAAGGTGCAG AAGCCGGCGA ACCCATGGGA GTTTTACATC 
AACAGCCAGC TAAATGCTCG AGTGCTGCGG AAAGAGCGTC ACCTGTTCAA CCAGCTCCAC 
TCCGCTCACC TGTTCACCAA TGGCAGTGTG CTTGTGGGGG AACTGCACAA CTGCGGGACT 
CTGCTGAGTG TGGTGAATCT GTATAAGAGC CGCAGTGAGA AGGTGATGCC CCAGCCGCTG 
GTGCTGTACT TCAGCATGTG TATCCTGCAG ATGGTGGAGC AGCTGCATCA AGCCAAAATC 
ATCCACGCCG ACATCAAACC TGACAACTTC CTGCTTGGAG AAAGGTTTCT GGAGAACGAG 
GATTTGGATG CTGAGAGTCT GGATCACGGC TTGGTTCTGA TAGATTTTGG TCAAAGTATT 
GATATGACCC TGTTCCCTGA GGGCACGGCC TTCACTGCCC GCTGCATGAC TTCTGGCTTC 
CAGTGCACAG AGATGCTGAG TGGCCGGCCG TGGAACTACC AGACTGATTA CTTTGGCATT 
GCCGGCACCG TGCACTGCAT GATCTTCGGG TCATACATGC AAGTGAGAAA TGAGGGTGGA 
GTTTGGAAAA CGAATGGCAT TTTTAAAAGG AATCCTCACA GTGATCTATG GCAGGAGTTT 
TTCCATGTGC TGCTGAATGT TCCGGACTGT AACTCTGCCT TATGTTTGCA GAGTTTGCGT 
GAGCGTCTGT CTGTGGTGCT GCAGGAAAAC TACAGCAACA AACTGCGCGG CCTTAAAAAC 
CGACTGGTGG TGCAGATCCT GGAGAGCCGC GGGGCTCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007259948.2
CDS	505..5349
Translation	MDINSYLQQFEATVSSYVGEDPLDPWDKFVEFLECRLAAEERSNLCVVLERLVQTFLQDKRYRNDPRYINHCIKCAGYYGEPIKLYSYVHSLGVGARAAGLYVAWAQQFESKGLFTQAEQVYQRAIENQAEPMDTIQEQYRLFQSRMGKSQAASDHVRNPLQNSQLMNQNPRHRETLQPHCKEPDNSPLPADRTIRIISRSENNPMPRSSSSVESVPMYCVKELECEGSELSFEELRARRYFSKRQQLELLRKQEEVIWKHEEEEEEVIRMKKLLEELNSKLSTEQQETKHTFLQQELNESCSPLDRSVRQPLTPAMAEAAAPTSVTKEQAACSDIHRLQHAPERPLLNGRKSESHLLWQSTRVPASRLSLSLSAASQLAHSSALSAAVNRPLDLDTQAPVPLQLQDQRTASVCESQAERSLLESCSQRLAVFERDRSEKTEMDGGDANADNVIMDMFQAPTLLQDSVFNTTSQVADDSFEKNCRVYGFAPSVANPPSSMSFRIYQDENVKIGSEEQQVEKSKPAARALMEIPVSKPNVTPVGPESLTDESAMWGPQYASITACPNHTRDFAMAAHLVSTPLHPTAPYTWDTELNQVENTARGFSGTEENLYQRQPAKLSPILEQSPSDEKPSEVPECSLRAQGTIVGEGVSLPQQNQTLTHDQTSCSIGRQTLVPLALHDHTAEASRALNSSTKPSWSVYQSPERTSQTGSNTEPVSNSMLSVKQQIAHQLNSSNLQLNSSSSIKPSWSIYQSPEKTSHTGSNRGLNSSSMLSVREPQPIQSNANGLQMNSLSCSLAEASNALNSSAKPNWSIYQSPEKTSQTDCNTEPTSNSILSVKQYQADQFNLPSMQANSSSSTKPSWTIYQSPEKSHQASFCAEPKQHSNSMHLAVGEPKQDQFSSTGLQVNPDRTMNSTTQSPERTQKTQFSGDLHSTSMHLVAEPKQDQLNSSAIQLNSSCSTKPSWSIYQSPERTSQMGHSREFKADQQVNSNQGFNSSSLHLMGELKPDQLNSSINMFSGQGEADILLPKKSFHRRKSEKQLNFESLQVSRPVSHQSCTLPESPKPQPRIIQDVPMSPDPIPGSDWLQMDSPVPLSEPNLDVMVRSMNRSQTIHRETPGAPVSRSKLSWSVNQTPGRPSELQYFLQSPEPMEESDPHQLSKLEESGLLLSKRSFNRRKSDKRVLEKSLLSSQRQSSNQNQDVPMSPEPISGLNWLCPESPARVTESDLDVMMTPQQKSKSVDASAQLLQCDVPMSPLQASIPDYTPSVLVSDPWDEDLIDSLLSSLKTPLTSYPNLSLCTRGLPTLTPKMSVQMGEDSLRVDFVLGQGAFATVYQATNLTTSQKFILKVQKPANPWEFYINSQLNARVLRKERHLFNQLHSAHLFTNGSVLVGELHNCGTLLSVVNLYKSRSEKVMPQPLVLYFSMCILQMVEQLHQAKIIHADIKPDNFLLGERFLENEDLDAESLDHGLVLIDFGQSIDMTLFPEGTAFTARCMTSGFQCTEMLSGRPWNYQTDYFGIAGTVHCMIFGSYMQVRNEGGVWKTNGIFKRNPHSDLWQEFFHVLLNVPDCNSALCLQSLRERLSVVLQENYSNKLRGLKNRLVVQILESRGARR

Target ORF information:

RefSeq Version	XM_007259886.3
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007259886.3

ORF Insert Sequence:

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CAACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCCTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGCGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACAACGTGA TCATGGACAT GTTCCAGGCT 
CCAACGCTGC TGCAGGACTC CGTCTTCAAT ACTACCAGCC AGGTGGCTGA TGACAGCTTT 
GAGAAAAACT GCAGAGTATA CGGTTTTGCC CCATCTGTAG CTAATCCACC CTCCTCAATG 
TCGTTCAGGA TATACCAGGA TGAAAATGTG AAGATTGGAA GTGAGGAACA ACAGGTGGAG 
AAGAGCAAAC CAGCAGCTCG AGCCCTGATG GAGATTCCGG TCTCCAAACC CAATGTCACT 
CCTGTAGGTC CGGAGTCTCT GACAGACGAG AGTGCGATGT GGGGTCCTCA ATATGCCTCC 
ATAACTGCCT GTCCAAATCA TACTCGTGAT TTTGCCATGG CTGCCCACCT GGTATCCACC 
CCCCTCCACC CCACTGCCCC GTACACCTGG GACACAGAGC TCAATCAGGT AGAAAACACT 
GCCAGAGGAT TTAGTGGGAC AGAGGAAAAC CTGTATCAGC GCCAGCCAGC CAAACTGAGT 
CCAATCCTGG AGCAGAGTCC TTCTGATGAG AAGCCGTCTG AAGTTCCTGA GTGCAGTCTG 
AGGGCTCAGG GCACCATCGT AGGAGAGGGA GTGAGTCTTC CTCAGCAGAA CCAGACGCTG 
ACCCACGACC AGACCAGCTG CAGCATCGGC AGGCAGACGC TGGTCCCCCT GGCTTTACAT 
GATCACACTG CCGAGGCCAG CAGAGCCCTC AACTCCAGCA CTAAACCTAG CTGGAGTGTC 
TACCAGAGCC CAGAGAGAAC CAGCCAGACA GGTTCTAACA CAGAACCTGT GTCCAACAGC 
ATGCTGTCTG TGAAACAGCA AATAGCTCAC CAGCTCAATT CATCTAATCT ACAGCTTAAT 
TCCAGCTCTA GCATCAAACC CAGCTGGAGC ATCTACCAGA GCCCAGAGAA AACCAGCCAT 
ACGGGCTCGA ACAGAGGGCT CAACTCAAGC AGCATGCTGT CTGTAAGAGA GCCTCAACCG 
ATCCAATCCA ATGCAAATGG CCTACAGATG AACTCTCTAT CTTGCTCCCT TGCTGAGGCC 
AGCAATGCCC TCAACTCCAG TGCCAAACCT AACTGGAGCA TCTACCAGAG CCCAGAGAAA 
ACCAGCCAGA CAGATTGTAA CACAGAACCG ACCTCTAACA GCATACTGTC GGTAAAGCAG 
TACCAAGCAG ACCAGTTCAA TTTACCTAGC ATGCAGGCTA ACTCCAGCTC CAGCACTAAA 
CCCAGCTGGA CCATCTACCA AAGCCCAGAG AAATCCCACC AGGCTAGTTT TTGTGCGGAA 
CCCAAACAAC ACTCAAACAG CATGCACTTG GCAGTGGGAG AACCCAAACA GGACCAGTTC 
AGCTCAACTG GCTTGCAGGT TAATCCAGAC AGAACCATGA ACTCCACCAC CCAAAGCCCA 
GAGAGAACCC AAAAGACTCA ATTTAGTGGG GACCTCCACT CAACCAGCAT GCATTTAGTG 
GCAGAACCCA AACAAGACCA GCTTAACTCA TCTGCCATTC AGCTGAACTC CAGCTGCAGC 
ACCAAACCCA GCTGGAGTAT TTACCAGAGC CCAGAGAGAA CCAGTCAAAT GGGTCACAGC 
AGGGAGTTCA AGGCAGACCA GCAGGTTAAC TCAAACCAAG GGTTCAACTC TTCTAGCCTG 
CATTTAATGG GAGAGCTAAA ACCAGACCAA CTCAATTCCT CCATCAATAT GTTTTCTGGT 
CAGGGTGAGG CTGATATTCT CTTGCCCAAG AAGTCTTTTC ACCGGCGAAA ATCCGAAAAA 
CAGCTGAACT TCGAGAGCCT TCAGGTGAGC CGACCTGTAT CACACCAGTC CTGCACGCTG 
CCAGAGAGCC CAAAACCACA GCCCAGAATA ATCCAGGATG TTCCCATGAG TCCAGACCCA 
ATCCCAGGGT CCGACTGGCT TCAGATGGAC AGTCCAGTCC CGCTCTCTGA ACCCAACCTG 
GATGTGATGG TCAGGTCAAT GAACAGATCC CAGACAATTC ACAGAGAAAC CCCCGGAGCT 
CCTGTTTCCA GGTCCAAACT CAGCTGGAGC GTGAACCAGA CCCCAGGGAG GCCATCTGAA 
CTACAGTACT TCCTGCAGAG TCCTGAGCCC ATGGAGGAAT CAGATCCGCA TCAGTTATCA 
AAGCTGGAAG AGTCTGGACT TCTGCTCTCC AAGAGATCCT TTAACAGGCG CAAATCGGAT 
AAGAGGGTAT TGGAGAAATC CCTCTTGTCG AGTCAGAGGC AGTCCTCCAA CCAAAACCAG 
GATGTTCCAA TGAGTCCGGA ACCAATTTCG GGGCTGAACT GGCTCTGTCC AGAGAGTCCA 
GCCCGTGTGA CTGAGTCTGA TCTGGACGTC ATGATGACAC CTCAGCAAAA ATCTAAATCA 
GTGGATGCTT CTGCCCAGCT GCTGCAGTGT GATGTTCCAA TGAGCCCCCT GCAGGCTTCC 
ATACCTGATT ACACTCCCTC AGTGTTGGTG TCTGACCCGT GGGATGAGGA CCTGATAGAT 
TCTTTACTCT CCAGCCTGAA GACGCCCCTC ACCTCATACC CGAACCTCAG CCTCTGCACT 
CGAGGCCTGC CCACACTCAC CCCCAAGATG TCCGTACAGA TGGGTGAGGA CTCTCTGCGG 
GTGGACTTTG TGTTGGGACA GGGTGCTTTC GCCACCGTTT ATCAGGCTAC CAACCTTACA 
ACCTCACAAA AATTCATCCT AAAGGTGCAG AAGCCGGCGA ACCCATGGGA GTTTTACATC 
AACAGCCAGC TAAATGCTCG AGTGCTGCGG AAAGAGCGTC ACCTGTTCAA CCAGCTCCAC 
TCCGCTCACC TGTTCACCAA TGGCAGTGTG CTTGTGGGGG AACTGCACAA CTGCGGGACT 
CTGCTGAGTG TGGTGAATCT GTATAAGAGC CGCAGTGAGA AGGTGATGCC CCAGCCGCTG 
GTGCTGTACT TCAGCATGTG TATCCTGCAG ATGGTGGAGC AGCTGCATCA AGCCAAAATC 
ATCCACGCCG ACATCAAACC TGACAACTTC CTGCTTGGAG AAAGGTTTCT GGAGAACGAG 
GATTTGGATG CTGAGAGTCT GGATCACGGC TTGGTTCTGA TAGATTTTGG TCAAAGTATT 
GATATGACCC TGTTCCCTGA GGGCACGGCC TTCACTGCCC GCTGCATGAC TTCTGGCTTC 
CAGTGCACAG AGATGCTGAG TGGCCGGCCG TGGAACTACC AGACTGATTA CTTTGGCATT 
GCCGGCACCG TGCACTGCAT GATCTTCGGG TCATACATGC AAGTGAGAAA TGAGGGTGGA 
GTTTGGAAAA CGAATGGCAT TTTTAAAAGG AATCCTCACA GTGATCTATG GCAGGAGTTT 
TTCCATGTGC TGCTGAATGT TCCGGACTGT AACTCTGCCT TATGTTTGCA GAGTTTGCGT 
GAGCGTCTGT CTGTGGTGCT GCAGGAAAAC TACAGCAACA AACTGCGCGG CCTTAAAAAC 
CGACTGGTGG TGCAGATCCT GGAGAGCCGC GGGGCTCGCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_015608508.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015463994.2 Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.

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CloneID	OAs123419
Clone ID Related Accession (Same CDS sequence)	XM_015608508.2
Accession Version	XM_015608508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_015608508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGT TCCAGCTTCC 
AGACTGAGCC TGTCTCTGAG CGCAGCTTCA CAACTTGCAC ACAGCAGTGC ACTCAGTGCA 
GCTGTGAATA GGCCATTGGA CCTGGACACA CAGGCTCCAG TTCCACTACA GCTGCAAGAC 
CAGAGAACCG CAAGCGTGTG TGAGAGCCAG GCTGAGCGTT CGCTTCTAGA AAGTTGCTCT 
CAGAGGCTGG CAGTGTTTGA AAGAGACAGA TCTGAAAAAA CAGAGATGGA TGGAGGAGAT 
GCTAATGCAG ACACATCTCA CGGAGGCTTT AATCATTCTC ATGTCACTCC TAACACATCT 
CTGGGTCTGA TTTCGGCGAC TCCTTCACGT GCACTCCCCT CTCCCACTGT CAACACTCGG 
GAGGCTCTGG ACGTGATCAT GGACATGTTC CAGGCTCCAA CGCTGCTGCA GGACTCCGTC 
TTCAATACTA CCAGCCAGGT GGCTGATGAC AGCTTTGAGA AAAACTGCAG AGTATACGGT 
TTTGCCCCAT CTGTAGCTAA TCCACCCTCC TCAATGTCGT TCAGGATATA CCAGGATGAA 
AATGTGAAGA TTGGAAGTGA GGAACAACAG GTGGAGAAGA GCAAACCAGC AGCTCGAGCC 
CTGATGGAGA TTCCGGTCTC CAAACCCAAT GTCACTCCTG TAGGTCCGGA GTCTCTGACA 
GACGAGAGTG CGATGTGGGG TCCTCAATAT GCCTCCATAA CTGCCTGTCC AAATCATACT 
CGTGATTTTG CCATGGCTGC CCACCTGGTA TCCACCCCCC TCCACCCCAC TGCCCCGTAC 
ACCTGGGACA CAGAGCTCAA TCAGGTAGAA AACACTGCCA GAGGATTTAG TGGGACAGAG 
GAAAACCTGT ATCAGCGCCA GCCAGCCAAA CTGAGTCCAA TCCTGGAGCA GAGTCCTTCT 
GATGAGAAGC CGTCTGAAGT TCCTGAGTGC AGTCTGAGGG CTCAGGGCAC CATCGTAGGA 
GAGGGAGTGA GTCTTCCTCA GCAGAACCAG ACGCTGACCC ACGACCAGAC CAGCTGCAGC 
ATCGGCAGGC AGACGCTGGT CCCCCTGGCT TTACATGATC ACACTGCCGA GGCCAGCAGA 
GCCCTCAACT CCAGCACTAA ACCTAGCTGG AGTGTCTACC AGAGCCCAGA GAGAACCAGC 
CAGACAGGTT CTAACACAGA ACCTGTGTCC AACAGCATGC TGTCTGTGAA ACAGCAAATA 
GCTCACCAGC TCAATTCATC TAATCTACAG CTTAATTCCA GCTCTAGCAT CAAACCCAGC 
TGGAGCATCT ACCAGAGCCC AGAGAAAACC AGCCATACGG GCTCGAACAG AGGGCTCAAC 
TCAAGCAGCA TGCTGTCTGT AAGAGAGCCT CAACCGATCC AATCCAATGC AAATGGCCTA 
CAGATGAACT CTCTATCTTG CTCCCTTGCT GAGGCCAGCA ATGCCCTCAA CTCCAGTGCC 
AAACCTAACT GGAGCATCTA CCAGAGCCCA GAGAAAACCA GCCAGACAGA TTGTAACACA 
GAACCGACCT CTAACAGCAT ACTGTCGGTA AAGCAGTACC AAGCAGACCA GTTCAATTTA 
CCTAGCATGC AGGCTAACTC CAGCTCCAGC ACTAAACCCA GCTGGACCAT CTACCAAAGC 
CCAGAGAAAT CCCACCAGGC TAGTTTTTGT GCGGAACCCA AACAACACTC AAACAGCATG 
CACTTGGCAG TGGGAGAACC CAAACAGGAC CAGTTCAGCT CAACTGGCTT GCAGGTTAAT 
CCAGACAGAA CCATGAACTC CACCACCCAA AGCCCAGAGA GAACCCAAAA GACTCAATTT 
AGTGGGGACC TCCACTCAAC CAGCATGCAT TTAGTGGCAG AACCCAAACA AGACCAGCTT 
AACTCATCTG CCATTCAGCT GAACTCCAGC TGCAGCACCA AACCCAGCTG GAGTATTTAC 
CAGAGCCCAG AGAGAACCAG TCAAATGGGT CACAGCAGGG AGTTCAAGGC AGACCAGCAG 
GTTAACTCAA ACCAAGGGTT CAACTCTTCT AGCCTGCATT TAATGGGAGA GCTAAAACCA 
GACCAACTCA ATTCCTCCAT CAATATGTTT TCTGGTCAGG GTGAGGCTGA TATTCTCTTG 
CCCAAGAAGT CTTTTCACCG GCGAAAATCC GAAAAACAGC TGAACTTCGA GAGCCTTCAG 
GTGAGCCGAC CTGTATCACA CCAGTCCTGC ACGCTGCCAG AGAGCCCAAA ACCACAGCCC 
AGAATAATCC AGGATGTTCC CATGAGTCCA GACCCAATCC CAGGGTCCGA CTGGCTTCAG 
ATGGACAGTC CAGTCCCGCT CTCTGAACCC AACCTGGATG TGATGGTCAG GTCAATGAAC 
AGATCCCAGA CAATTCACAG AGAAACCCCC GGAGCTCCTG TTTCCAGGTC CAAACTCAGC 
TGGAGCGTGA ACCAGACCCC AGGGAGGCCA TCTGAACTAC AGTACTTCCT GCAGAGTCCT 
GAGCCCATGG AGGAATCAGA TCCGCATCAG TTATCAAAGC TGGAAGAGTC TGGACTTCTG 
CTCTCCAAGA GATCCTTTAA CAGGCGCAAA TCGGATAAGA GGGTATTGGA GAAATCCCTC 
TTGTCGAGTC AGAGGCAGTC CTCCAACCAA AACCAGGATG TTCCAATGAG TCCGGAACCA 
ATTTCGGGGC TGAACTGGCT CTGTCCAGAG AGTCCAGCCC GTGTGACTGA GTCTGATCTG 
GACGTCATGA TGACACCTCA GCAAAAATCT AAATCAGTGG ATGCTTCTGC CCAGCTGCTG 
CAGTGTGATG TTCCAATGAG CCCCCTGCAG GCTTCCATAC CTGATTACAC TCCCTCAGTG 
TTGGTGTCTG ACCCGTGGGA TGAGGACCTG ATAGATTCTT TACTCTCCAG CCTGAAGACG 
CCCCTCACCT CATACCCGAA CCTCAGCCTC TGCACTCGAG GCCTGCCCAC ACTCACCCCC 
AAGATGTCCG TACAGATGGG TGAGGACTCT CTGCGGGTGG ACTTTGTGTT GGGACAGGGT 
GCTTTCGCCA CCGTTTATCA GGCTACCAAC CTTACAACCT CACAAAAATT CATCCTAAAG 
GTGCAGAAGC CGGCGAACCC ATGGGAGTTT TACATCAACA GCCAGCTAAA TGCTCGAGTG 
CTGCGGAAAG AGCGTCACCT GTTCAACCAG CTCCACTCCG CTCACCTGTT CACCAATGGC 
AGTGTGCTTG TGGGGGAACT GCACAACTGC GGGACTCTGC TGAGTGTGGT GAATCTGTAT 
AAGAGCCGCA GTGAGAAGGT GATGCCCCAG CCGCTGGTGC TGTACTTCAG CATGTGTATC 
CTGCAGATGG TGGAGCAGCT GCATCAAGCC AAAATCATCC ACGCCGACAT CAAACCTGAC 
AACTTCCTGC TTGGAGAAAG GTTTCTGGAG AACGAGGATT TGGATGCTGA GAGTCTGGAT 
CACGGCTTGG TTCTGATAGA TTTTGGTCAA AGTATTGATA TGACCCTGTT CCCTGAGGGC 
ACGGCCTTCA CTGCCCGCTG CATGACTTCT GGCTTCCAGT GCACAGAGAT GCTGAGTGGC 
CGGCCGTGGA ACTACCAGAC TGATTACTTT GGCATTGCCG GCACCGTGCA CTGCATGATC 
TTCGGGTCAT ACATGCAAGT GAGAAATGAG GGTGGAGTTT GGAAAACGAA TGGCATTTTT 
AAAAGGAATC CTCACAGTGA TCTATGGCAG GAGTTTTTCC ATGTGCTGCT GAATGTTCCG 
GACTGTAACT CTGCCTTATG TTTGCAGAGT TTGCGTGAGC GTCTGTCTGT GGTGCTGCAG 
GAAAACTACA GCAACAAACT GCGCGGCCTT AAAAACCGAC TGGTGGTGCA GATCCTGGAG 
AGCCGCGGGG CTCGCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015463994.2
CDS	505..5265
Translation	MDINSYLQQFEATVSSYVGEDPLDPWDKFVEFLECRLAAEERSNLCVVLERLVQTFLQDKRYRNDPRYINHCIKCAGYYGEPIKLYSYVHSLGVGARAAGLYVAWAQQFESKGLFTQAEQVYQRAIENQAEPMDTIQEQYRLFQSRMGKSQAASDHVRNPLQNSQLMNQNPRHRETLQPHCKEPDNSPLPADRTIRIISRSENNPMPRSSSSVESVPMYCVKELECEGSELSFEELRARRYFSKRQQLELLRKQEEVIWKHEEEEEEVIRMKKLLEELNSKLSTEQQETKHTFLQQVPASRLSLSLSAASQLAHSSALSAAVNRPLDLDTQAPVPLQLQDQRTASVCESQAERSLLESCSQRLAVFERDRSEKTEMDGGDANADTSHGGFNHSHVTPNTSLGLISATPSRALPSPTVNTREALDVIMDMFQAPTLLQDSVFNTTSQVADDSFEKNCRVYGFAPSVANPPSSMSFRIYQDENVKIGSEEQQVEKSKPAARALMEIPVSKPNVTPVGPESLTDESAMWGPQYASITACPNHTRDFAMAAHLVSTPLHPTAPYTWDTELNQVENTARGFSGTEENLYQRQPAKLSPILEQSPSDEKPSEVPECSLRAQGTIVGEGVSLPQQNQTLTHDQTSCSIGRQTLVPLALHDHTAEASRALNSSTKPSWSVYQSPERTSQTGSNTEPVSNSMLSVKQQIAHQLNSSNLQLNSSSSIKPSWSIYQSPEKTSHTGSNRGLNSSSMLSVREPQPIQSNANGLQMNSLSCSLAEASNALNSSAKPNWSIYQSPEKTSQTDCNTEPTSNSILSVKQYQADQFNLPSMQANSSSSTKPSWTIYQSPEKSHQASFCAEPKQHSNSMHLAVGEPKQDQFSSTGLQVNPDRTMNSTTQSPERTQKTQFSGDLHSTSMHLVAEPKQDQLNSSAIQLNSSCSTKPSWSIYQSPERTSQMGHSREFKADQQVNSNQGFNSSSLHLMGELKPDQLNSSINMFSGQGEADILLPKKSFHRRKSEKQLNFESLQVSRPVSHQSCTLPESPKPQPRIIQDVPMSPDPIPGSDWLQMDSPVPLSEPNLDVMVRSMNRSQTIHRETPGAPVSRSKLSWSVNQTPGRPSELQYFLQSPEPMEESDPHQLSKLEESGLLLSKRSFNRRKSDKRVLEKSLLSSQRQSSNQNQDVPMSPEPISGLNWLCPESPARVTESDLDVMMTPQQKSKSVDASAQLLQCDVPMSPLQASIPDYTPSVLVSDPWDEDLIDSLLSSLKTPLTSYPNLSLCTRGLPTLTPKMSVQMGEDSLRVDFVLGQGAFATVYQATNLTTSQKFILKVQKPANPWEFYINSQLNARVLRKERHLFNQLHSAHLFTNGSVLVGELHNCGTLLSVVNLYKSRSEKVMPQPLVLYFSMCILQMVEQLHQAKIIHADIKPDNFLLGERFLENEDLDAESLDHGLVLIDFGQSIDMTLFPEGTAFTARCMTSGFQCTEMLSGRPWNYQTDYFGIAGTVHCMIFGSYMQVRNEGGVWKTNGIFKRNPHSDLWQEFFHVLLNVPDCNSALCLQSLRERLSVVLQENYSNKLRGLKNRLVVQILESRGARR

Target ORF information:

RefSeq Version	XM_015608508.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015608508.2

ORF Insert Sequence:

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGT TCCAGCTTCC 
AGACTGAGCC TGTCTCTGAG CGCAGCTTCA CAACTTGCAC ACAGCAGTGC ACTCAGTGCA 
GCTGTGAATA GGCCATTGGA CCTGGACACA CAGGCTCCAG TTCCACTACA GCTGCAAGAC 
CAGAGAACCG CAAGCGTGTG TGAGAGCCAG GCTGAGCGTT CGCTTCTAGA AAGTTGCTCT 
CAGAGGCTGG CAGTGTTTGA AAGAGACAGA TCTGAAAAAA CAGAGATGGA TGGAGGAGAT 
GCTAATGCAG ACACATCTCA CGGAGGCTTT AATCATTCTC ATGTCACTCC TAACACATCT 
CTGGGTCTGA TTTCGGCGAC TCCTTCACGT GCACTCCCCT CTCCCACTGT CAACACTCGG 
GAGGCTCTGG ACGTGATCAT GGACATGTTC CAGGCTCCAA CGCTGCTGCA GGACTCCGTC 
TTCAATACTA CCAGCCAGGT GGCTGATGAC AGCTTTGAGA AAAACTGCAG AGTATACGGT 
TTTGCCCCAT CTGTAGCTAA TCCACCCTCC TCAATGTCGT TCAGGATATA CCAGGATGAA 
AATGTGAAGA TTGGAAGTGA GGAACAACAG GTGGAGAAGA GCAAACCAGC AGCTCGAGCC 
CTGATGGAGA TTCCGGTCTC CAAACCCAAT GTCACTCCTG TAGGTCCGGA GTCTCTGACA 
GACGAGAGTG CGATGTGGGG TCCTCAATAT GCCTCCATAA CTGCCTGTCC AAATCATACT 
CGTGATTTTG CCATGGCTGC CCACCTGGTA TCCACCCCCC TCCACCCCAC TGCCCCGTAC 
ACCTGGGACA CAGAGCTCAA TCAGGTAGAA AACACTGCCA GAGGATTTAG TGGGACAGAG 
GAAAACCTGT ATCAGCGCCA GCCAGCCAAA CTGAGTCCAA TCCTGGAGCA GAGTCCTTCT 
GATGAGAAGC CGTCTGAAGT TCCTGAGTGC AGTCTGAGGG CTCAGGGCAC CATCGTAGGA 
GAGGGAGTGA GTCTTCCTCA GCAGAACCAG ACGCTGACCC ACGACCAGAC CAGCTGCAGC 
ATCGGCAGGC AGACGCTGGT CCCCCTGGCT TTACATGATC ACACTGCCGA GGCCAGCAGA 
GCCCTCAACT CCAGCACTAA ACCTAGCTGG AGTGTCTACC AGAGCCCAGA GAGAACCAGC 
CAGACAGGTT CTAACACAGA ACCTGTGTCC AACAGCATGC TGTCTGTGAA ACAGCAAATA 
GCTCACCAGC TCAATTCATC TAATCTACAG CTTAATTCCA GCTCTAGCAT CAAACCCAGC 
TGGAGCATCT ACCAGAGCCC AGAGAAAACC AGCCATACGG GCTCGAACAG AGGGCTCAAC 
TCAAGCAGCA TGCTGTCTGT AAGAGAGCCT CAACCGATCC AATCCAATGC AAATGGCCTA 
CAGATGAACT CTCTATCTTG CTCCCTTGCT GAGGCCAGCA ATGCCCTCAA CTCCAGTGCC 
AAACCTAACT GGAGCATCTA CCAGAGCCCA GAGAAAACCA GCCAGACAGA TTGTAACACA 
GAACCGACCT CTAACAGCAT ACTGTCGGTA AAGCAGTACC AAGCAGACCA GTTCAATTTA 
CCTAGCATGC AGGCTAACTC CAGCTCCAGC ACTAAACCCA GCTGGACCAT CTACCAAAGC 
CCAGAGAAAT CCCACCAGGC TAGTTTTTGT GCGGAACCCA AACAACACTC AAACAGCATG 
CACTTGGCAG TGGGAGAACC CAAACAGGAC CAGTTCAGCT CAACTGGCTT GCAGGTTAAT 
CCAGACAGAA CCATGAACTC CACCACCCAA AGCCCAGAGA GAACCCAAAA GACTCAATTT 
AGTGGGGACC TCCACTCAAC CAGCATGCAT TTAGTGGCAG AACCCAAACA AGACCAGCTT 
AACTCATCTG CCATTCAGCT GAACTCCAGC TGCAGCACCA AACCCAGCTG GAGTATTTAC 
CAGAGCCCAG AGAGAACCAG TCAAATGGGT CACAGCAGGG AGTTCAAGGC AGACCAGCAG 
GTTAACTCAA ACCAAGGGTT CAACTCTTCT AGCCTGCATT TAATGGGAGA GCTAAAACCA 
GACCAACTCA ATTCCTCCAT CAATATGTTT TCTGGTCAGG GTGAGGCTGA TATTCTCTTG 
CCCAAGAAGT CTTTTCACCG GCGAAAATCC GAAAAACAGC TGAACTTCGA GAGCCTTCAG 
GTGAGCCGAC CTGTATCACA CCAGTCCTGC ACGCTGCCAG AGAGCCCAAA ACCACAGCCC 
AGAATAATCC AGGATGTTCC CATGAGTCCA GACCCAATCC CAGGGTCCGA CTGGCTTCAG 
ATGGACAGTC CAGTCCCGCT CTCTGAACCC AACCTGGATG TGATGGTCAG GTCAATGAAC 
AGATCCCAGA CAATTCACAG AGAAACCCCC GGAGCTCCTG TTTCCAGGTC CAAACTCAGC 
TGGAGCGTGA ACCAGACCCC AGGGAGGCCA TCTGAACTAC AGTACTTCCT GCAGAGTCCT 
GAGCCCATGG AGGAATCAGA TCCGCATCAG TTATCAAAGC TGGAAGAGTC TGGACTTCTG 
CTCTCCAAGA GATCCTTTAA CAGGCGCAAA TCGGATAAGA GGGTATTGGA GAAATCCCTC 
TTGTCGAGTC AGAGGCAGTC CTCCAACCAA AACCAGGATG TTCCAATGAG TCCGGAACCA 
ATTTCGGGGC TGAACTGGCT CTGTCCAGAG AGTCCAGCCC GTGTGACTGA GTCTGATCTG 
GACGTCATGA TGACACCTCA GCAAAAATCT AAATCAGTGG ATGCTTCTGC CCAGCTGCTG 
CAGTGTGATG TTCCAATGAG CCCCCTGCAG GCTTCCATAC CTGATTACAC TCCCTCAGTG 
TTGGTGTCTG ACCCGTGGGA TGAGGACCTG ATAGATTCTT TACTCTCCAG CCTGAAGACG 
CCCCTCACCT CATACCCGAA CCTCAGCCTC TGCACTCGAG GCCTGCCCAC ACTCACCCCC 
AAGATGTCCG TACAGATGGG TGAGGACTCT CTGCGGGTGG ACTTTGTGTT GGGACAGGGT 
GCTTTCGCCA CCGTTTATCA GGCTACCAAC CTTACAACCT CACAAAAATT CATCCTAAAG 
GTGCAGAAGC CGGCGAACCC ATGGGAGTTT TACATCAACA GCCAGCTAAA TGCTCGAGTG 
CTGCGGAAAG AGCGTCACCT GTTCAACCAG CTCCACTCCG CTCACCTGTT CACCAATGGC 
AGTGTGCTTG TGGGGGAACT GCACAACTGC GGGACTCTGC TGAGTGTGGT GAATCTGTAT 
AAGAGCCGCA GTGAGAAGGT GATGCCCCAG CCGCTGGTGC TGTACTTCAG CATGTGTATC 
CTGCAGATGG TGGAGCAGCT GCATCAAGCC AAAATCATCC ACGCCGACAT CAAACCTGAC 
AACTTCCTGC TTGGAGAAAG GTTTCTGGAG AACGAGGATT TGGATGCTGA GAGTCTGGAT 
CACGGCTTGG TTCTGATAGA TTTTGGTCAA AGTATTGATA TGACCCTGTT CCCTGAGGGC 
ACGGCCTTCA CTGCCCGCTG CATGACTTCT GGCTTCCAGT GCACAGAGAT GCTGAGTGGC 
CGGCCGTGGA ACTACCAGAC TGATTACTTT GGCATTGCCG GCACCGTGCA CTGCATGATC 
TTCGGGTCAT ACATGCAAGT GAGAAATGAG GGTGGAGTTT GGAAAACGAA TGGCATTTTT 
AAAAGGAATC CTCACAGTGA TCTATGGCAG GAGTTTTTCC ATGTGCTGCT GAATGTTCCG 
GACTGTAACT CTGCCTTATG TTTGCAGAGT TTGCGTGAGC GTCTGTCTGT GGTGCTGCAG 
GAAAACTACA GCAACAAACT GCGCGGCCTT AAAAACCGAC TGGTGGTGCA GATCCTGGAG 
AGCCGCGGGG CTCGCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_007259887.3 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007259949.2 Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X5, mRNA.

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CloneID	OAs123420
Clone ID Related Accession (Same CDS sequence)	XM_007259887.3
Accession Version	XM_007259887.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_007259887.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGT TCCAGCTTCC 
AGACTGAGCC TGTCTCTGAG CGCAGCTTCA CAACTTGCAC ACAGCAGTGC ACTCAGTGCA 
GCTGTGAATA GGCCATTGGA CCTGGACACA CAGGCTCCAG TTCCACTACA GCTGCAAGAC 
CAGAGAACCG CAAGCGTGTG TGAGAGCCAG GCTGAGCGTT CGCTTCTAGA AAGTTGCTCT 
CAGAGGCTGG CAGTGTTTGA AAGAGACAGA TCTGAAAAAA CAGAGATGGA TGGAGGAGAT 
GCTAATGCAG ACAACGTGAT CATGGACATG TTCCAGGCTC CAACGCTGCT GCAGGACTCC 
GTCTTCAATA CTACCAGCCA GGTGGCTGAT GACAGCTTTG AGAAAAACTG CAGAGTATAC 
GGTTTTGCCC CATCTGTAGC TAATCCACCC TCCTCAATGT CGTTCAGGAT ATACCAGGAT 
GAAAATGTGA AGATTGGAAG TGAGGAACAA CAGGTGGAGA AGAGCAAACC AGCAGCTCGA 
GCCCTGATGG AGATTCCGGT CTCCAAACCC AATGTCACTC CTGTAGGTCC GGAGTCTCTG 
ACAGACGAGA GTGCGATGTG GGGTCCTCAA TATGCCTCCA TAACTGCCTG TCCAAATCAT 
ACTCGTGATT TTGCCATGGC TGCCCACCTG GTATCCACCC CCCTCCACCC CACTGCCCCG 
TACACCTGGG ACACAGAGCT CAATCAGGTA GAAAACACTG CCAGAGGATT TAGTGGGACA 
GAGGAAAACC TGTATCAGCG CCAGCCAGCC AAACTGAGTC CAATCCTGGA GCAGAGTCCT 
TCTGATGAGA AGCCGTCTGA AGTTCCTGAG TGCAGTCTGA GGGCTCAGGG CACCATCGTA 
GGAGAGGGAG TGAGTCTTCC TCAGCAGAAC CAGACGCTGA CCCACGACCA GACCAGCTGC 
AGCATCGGCA GGCAGACGCT GGTCCCCCTG GCTTTACATG ATCACACTGC CGAGGCCAGC 
AGAGCCCTCA ACTCCAGCAC TAAACCTAGC TGGAGTGTCT ACCAGAGCCC AGAGAGAACC 
AGCCAGACAG GTTCTAACAC AGAACCTGTG TCCAACAGCA TGCTGTCTGT GAAACAGCAA 
ATAGCTCACC AGCTCAATTC ATCTAATCTA CAGCTTAATT CCAGCTCTAG CATCAAACCC 
AGCTGGAGCA TCTACCAGAG CCCAGAGAAA ACCAGCCATA CGGGCTCGAA CAGAGGGCTC 
AACTCAAGCA GCATGCTGTC TGTAAGAGAG CCTCAACCGA TCCAATCCAA TGCAAATGGC 
CTACAGATGA ACTCTCTATC TTGCTCCCTT GCTGAGGCCA GCAATGCCCT CAACTCCAGT 
GCCAAACCTA ACTGGAGCAT CTACCAGAGC CCAGAGAAAA CCAGCCAGAC AGATTGTAAC 
ACAGAACCGA CCTCTAACAG CATACTGTCG GTAAAGCAGT ACCAAGCAGA CCAGTTCAAT 
TTACCTAGCA TGCAGGCTAA CTCCAGCTCC AGCACTAAAC CCAGCTGGAC CATCTACCAA 
AGCCCAGAGA AATCCCACCA GGCTAGTTTT TGTGCGGAAC CCAAACAACA CTCAAACAGC 
ATGCACTTGG CAGTGGGAGA ACCCAAACAG GACCAGTTCA GCTCAACTGG CTTGCAGGTT 
AATCCAGACA GAACCATGAA CTCCACCACC CAAAGCCCAG AGAGAACCCA AAAGACTCAA 
TTTAGTGGGG ACCTCCACTC AACCAGCATG CATTTAGTGG CAGAACCCAA ACAAGACCAG 
CTTAACTCAT CTGCCATTCA GCTGAACTCC AGCTGCAGCA CCAAACCCAG CTGGAGTATT 
TACCAGAGCC CAGAGAGAAC CAGTCAAATG GGTCACAGCA GGGAGTTCAA GGCAGACCAG 
CAGGTTAACT CAAACCAAGG GTTCAACTCT TCTAGCCTGC ATTTAATGGG AGAGCTAAAA 
CCAGACCAAC TCAATTCCTC CATCAATATG TTTTCTGGTC AGGGTGAGGC TGATATTCTC 
TTGCCCAAGA AGTCTTTTCA CCGGCGAAAA TCCGAAAAAC AGCTGAACTT CGAGAGCCTT 
CAGGTGAGCC GACCTGTATC ACACCAGTCC TGCACGCTGC CAGAGAGCCC AAAACCACAG 
CCCAGAATAA TCCAGGATGT TCCCATGAGT CCAGACCCAA TCCCAGGGTC CGACTGGCTT 
CAGATGGACA GTCCAGTCCC GCTCTCTGAA CCCAACCTGG ATGTGATGGT CAGGTCAATG 
AACAGATCCC AGACAATTCA CAGAGAAACC CCCGGAGCTC CTGTTTCCAG GTCCAAACTC 
AGCTGGAGCG TGAACCAGAC CCCAGGGAGG CCATCTGAAC TACAGTACTT CCTGCAGAGT 
CCTGAGCCCA TGGAGGAATC AGATCCGCAT CAGTTATCAA AGCTGGAAGA GTCTGGACTT 
CTGCTCTCCA AGAGATCCTT TAACAGGCGC AAATCGGATA AGAGGGTATT GGAGAAATCC 
CTCTTGTCGA GTCAGAGGCA GTCCTCCAAC CAAAACCAGG ATGTTCCAAT GAGTCCGGAA 
CCAATTTCGG GGCTGAACTG GCTCTGTCCA GAGAGTCCAG CCCGTGTGAC TGAGTCTGAT 
CTGGACGTCA TGATGACACC TCAGCAAAAA TCTAAATCAG TGGATGCTTC TGCCCAGCTG 
CTGCAGTGTG ATGTTCCAAT GAGCCCCCTG CAGGCTTCCA TACCTGATTA CACTCCCTCA 
GTGTTGGTGT CTGACCCGTG GGATGAGGAC CTGATAGATT CTTTACTCTC CAGCCTGAAG 
ACGCCCCTCA CCTCATACCC GAACCTCAGC CTCTGCACTC GAGGCCTGCC CACACTCACC 
CCCAAGATGT CCGTACAGAT GGGTGAGGAC TCTCTGCGGG TGGACTTTGT GTTGGGACAG 
GGTGCTTTCG CCACCGTTTA TCAGGCTACC AACCTTACAA CCTCACAAAA ATTCATCCTA 
AAGGTGCAGA AGCCGGCGAA CCCATGGGAG TTTTACATCA ACAGCCAGCT AAATGCTCGA 
GTGCTGCGGA AAGAGCGTCA CCTGTTCAAC CAGCTCCACT CCGCTCACCT GTTCACCAAT 
GGCAGTGTGC TTGTGGGGGA ACTGCACAAC TGCGGGACTC TGCTGAGTGT GGTGAATCTG 
TATAAGAGCC GCAGTGAGAA GGTGATGCCC CAGCCGCTGG TGCTGTACTT CAGCATGTGT 
ATCCTGCAGA TGGTGGAGCA GCTGCATCAA GCCAAAATCA TCCACGCCGA CATCAAACCT 
GACAACTTCC TGCTTGGAGA AAGGTTTCTG GAGAACGAGG ATTTGGATGC TGAGAGTCTG 
GATCACGGCT TGGTTCTGAT AGATTTTGGT CAAAGTATTG ATATGACCCT GTTCCCTGAG 
GGCACGGCCT TCACTGCCCG CTGCATGACT TCTGGCTTCC AGTGCACAGA GATGCTGAGT 
GGCCGGCCGT GGAACTACCA GACTGATTAC TTTGGCATTG CCGGCACCGT GCACTGCATG 
ATCTTCGGGT CATACATGCA AGTGAGAAAT GAGGGTGGAG TTTGGAAAAC GAATGGCATT 
TTTAAAAGGA ATCCTCACAG TGATCTATGG CAGGAGTTTT TCCATGTGCT GCTGAATGTT 
CCGGACTGTA ACTCTGCCTT ATGTTTGCAG AGTTTGCGTG AGCGTCTGTC TGTGGTGCTG 
CAGGAAAACT ACAGCAACAA ACTGCGCGGC CTTAAAAACC GACTGGTGGT GCAGATCCTG 
GAGAGCCGCG GGGCTCGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007259949.2
CDS	505..5148
Translation	MDINSYLQQFEATVSSYVGEDPLDPWDKFVEFLECRLAAEERSNLCVVLERLVQTFLQDKRYRNDPRYINHCIKCAGYYGEPIKLYSYVHSLGVGARAAGLYVAWAQQFESKGLFTQAEQVYQRAIENQAEPMDTIQEQYRLFQSRMGKSQAASDHVRNPLQNSQLMNQNPRHRETLQPHCKEPDNSPLPADRTIRIISRSENNPMPRSSSSVESVPMYCVKELECEGSELSFEELRARRYFSKRQQLELLRKQEEVIWKHEEEEEEVIRMKKLLEELNSKLSTEQQETKHTFLQQVPASRLSLSLSAASQLAHSSALSAAVNRPLDLDTQAPVPLQLQDQRTASVCESQAERSLLESCSQRLAVFERDRSEKTEMDGGDANADNVIMDMFQAPTLLQDSVFNTTSQVADDSFEKNCRVYGFAPSVANPPSSMSFRIYQDENVKIGSEEQQVEKSKPAARALMEIPVSKPNVTPVGPESLTDESAMWGPQYASITACPNHTRDFAMAAHLVSTPLHPTAPYTWDTELNQVENTARGFSGTEENLYQRQPAKLSPILEQSPSDEKPSEVPECSLRAQGTIVGEGVSLPQQNQTLTHDQTSCSIGRQTLVPLALHDHTAEASRALNSSTKPSWSVYQSPERTSQTGSNTEPVSNSMLSVKQQIAHQLNSSNLQLNSSSSIKPSWSIYQSPEKTSHTGSNRGLNSSSMLSVREPQPIQSNANGLQMNSLSCSLAEASNALNSSAKPNWSIYQSPEKTSQTDCNTEPTSNSILSVKQYQADQFNLPSMQANSSSSTKPSWTIYQSPEKSHQASFCAEPKQHSNSMHLAVGEPKQDQFSSTGLQVNPDRTMNSTTQSPERTQKTQFSGDLHSTSMHLVAEPKQDQLNSSAIQLNSSCSTKPSWSIYQSPERTSQMGHSREFKADQQVNSNQGFNSSSLHLMGELKPDQLNSSINMFSGQGEADILLPKKSFHRRKSEKQLNFESLQVSRPVSHQSCTLPESPKPQPRIIQDVPMSPDPIPGSDWLQMDSPVPLSEPNLDVMVRSMNRSQTIHRETPGAPVSRSKLSWSVNQTPGRPSELQYFLQSPEPMEESDPHQLSKLEESGLLLSKRSFNRRKSDKRVLEKSLLSSQRQSSNQNQDVPMSPEPISGLNWLCPESPARVTESDLDVMMTPQQKSKSVDASAQLLQCDVPMSPLQASIPDYTPSVLVSDPWDEDLIDSLLSSLKTPLTSYPNLSLCTRGLPTLTPKMSVQMGEDSLRVDFVLGQGAFATVYQATNLTTSQKFILKVQKPANPWEFYINSQLNARVLRKERHLFNQLHSAHLFTNGSVLVGELHNCGTLLSVVNLYKSRSEKVMPQPLVLYFSMCILQMVEQLHQAKIIHADIKPDNFLLGERFLENEDLDAESLDHGLVLIDFGQSIDMTLFPEGTAFTARCMTSGFQCTEMLSGRPWNYQTDYFGIAGTVHCMIFGSYMQVRNEGGVWKTNGIFKRNPHSDLWQEFFHVLLNVPDCNSALCLQSLRERLSVVLQENYSNKLRGLKNRLVVQILESRGARR

Target ORF information:

RefSeq Version	XM_007259887.3
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007259887.3

ORF Insert Sequence:

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGT TCCAGCTTCC 
AGACTGAGCC TGTCTCTGAG CGCAGCTTCA CAACTTGCAC ACAGCAGTGC ACTCAGTGCA 
GCTGTGAATA GGCCATTGGA CCTGGACACA CAGGCTCCAG TTCCACTACA GCTGCAAGAC 
CAGAGAACCG CAAGCGTGTG TGAGAGCCAG GCTGAGCGTT CGCTTCTAGA AAGTTGCTCT 
CAGAGGCTGG CAGTGTTTGA AAGAGACAGA TCTGAAAAAA CAGAGATGGA TGGAGGAGAT 
GCTAATGCAG ACAACGTGAT CATGGACATG TTCCAGGCTC CAACGCTGCT GCAGGACTCC 
GTCTTCAATA CTACCAGCCA GGTGGCTGAT GACAGCTTTG AGAAAAACTG CAGAGTATAC 
GGTTTTGCCC CATCTGTAGC TAATCCACCC TCCTCAATGT CGTTCAGGAT ATACCAGGAT 
GAAAATGTGA AGATTGGAAG TGAGGAACAA CAGGTGGAGA AGAGCAAACC AGCAGCTCGA 
GCCCTGATGG AGATTCCGGT CTCCAAACCC AATGTCACTC CTGTAGGTCC GGAGTCTCTG 
ACAGACGAGA GTGCGATGTG GGGTCCTCAA TATGCCTCCA TAACTGCCTG TCCAAATCAT 
ACTCGTGATT TTGCCATGGC TGCCCACCTG GTATCCACCC CCCTCCACCC CACTGCCCCG 
TACACCTGGG ACACAGAGCT CAATCAGGTA GAAAACACTG CCAGAGGATT TAGTGGGACA 
GAGGAAAACC TGTATCAGCG CCAGCCAGCC AAACTGAGTC CAATCCTGGA GCAGAGTCCT 
TCTGATGAGA AGCCGTCTGA AGTTCCTGAG TGCAGTCTGA GGGCTCAGGG CACCATCGTA 
GGAGAGGGAG TGAGTCTTCC TCAGCAGAAC CAGACGCTGA CCCACGACCA GACCAGCTGC 
AGCATCGGCA GGCAGACGCT GGTCCCCCTG GCTTTACATG ATCACACTGC CGAGGCCAGC 
AGAGCCCTCA ACTCCAGCAC TAAACCTAGC TGGAGTGTCT ACCAGAGCCC AGAGAGAACC 
AGCCAGACAG GTTCTAACAC AGAACCTGTG TCCAACAGCA TGCTGTCTGT GAAACAGCAA 
ATAGCTCACC AGCTCAATTC ATCTAATCTA CAGCTTAATT CCAGCTCTAG CATCAAACCC 
AGCTGGAGCA TCTACCAGAG CCCAGAGAAA ACCAGCCATA CGGGCTCGAA CAGAGGGCTC 
AACTCAAGCA GCATGCTGTC TGTAAGAGAG CCTCAACCGA TCCAATCCAA TGCAAATGGC 
CTACAGATGA ACTCTCTATC TTGCTCCCTT GCTGAGGCCA GCAATGCCCT CAACTCCAGT 
GCCAAACCTA ACTGGAGCAT CTACCAGAGC CCAGAGAAAA CCAGCCAGAC AGATTGTAAC 
ACAGAACCGA CCTCTAACAG CATACTGTCG GTAAAGCAGT ACCAAGCAGA CCAGTTCAAT 
TTACCTAGCA TGCAGGCTAA CTCCAGCTCC AGCACTAAAC CCAGCTGGAC CATCTACCAA 
AGCCCAGAGA AATCCCACCA GGCTAGTTTT TGTGCGGAAC CCAAACAACA CTCAAACAGC 
ATGCACTTGG CAGTGGGAGA ACCCAAACAG GACCAGTTCA GCTCAACTGG CTTGCAGGTT 
AATCCAGACA GAACCATGAA CTCCACCACC CAAAGCCCAG AGAGAACCCA AAAGACTCAA 
TTTAGTGGGG ACCTCCACTC AACCAGCATG CATTTAGTGG CAGAACCCAA ACAAGACCAG 
CTTAACTCAT CTGCCATTCA GCTGAACTCC AGCTGCAGCA CCAAACCCAG CTGGAGTATT 
TACCAGAGCC CAGAGAGAAC CAGTCAAATG GGTCACAGCA GGGAGTTCAA GGCAGACCAG 
CAGGTTAACT CAAACCAAGG GTTCAACTCT TCTAGCCTGC ATTTAATGGG AGAGCTAAAA 
CCAGACCAAC TCAATTCCTC CATCAATATG TTTTCTGGTC AGGGTGAGGC TGATATTCTC 
TTGCCCAAGA AGTCTTTTCA CCGGCGAAAA TCCGAAAAAC AGCTGAACTT CGAGAGCCTT 
CAGGTGAGCC GACCTGTATC ACACCAGTCC TGCACGCTGC CAGAGAGCCC AAAACCACAG 
CCCAGAATAA TCCAGGATGT TCCCATGAGT CCAGACCCAA TCCCAGGGTC CGACTGGCTT 
CAGATGGACA GTCCAGTCCC GCTCTCTGAA CCCAACCTGG ATGTGATGGT CAGGTCAATG 
AACAGATCCC AGACAATTCA CAGAGAAACC CCCGGAGCTC CTGTTTCCAG GTCCAAACTC 
AGCTGGAGCG TGAACCAGAC CCCAGGGAGG CCATCTGAAC TACAGTACTT CCTGCAGAGT 
CCTGAGCCCA TGGAGGAATC AGATCCGCAT CAGTTATCAA AGCTGGAAGA GTCTGGACTT 
CTGCTCTCCA AGAGATCCTT TAACAGGCGC AAATCGGATA AGAGGGTATT GGAGAAATCC 
CTCTTGTCGA GTCAGAGGCA GTCCTCCAAC CAAAACCAGG ATGTTCCAAT GAGTCCGGAA 
CCAATTTCGG GGCTGAACTG GCTCTGTCCA GAGAGTCCAG CCCGTGTGAC TGAGTCTGAT 
CTGGACGTCA TGATGACACC TCAGCAAAAA TCTAAATCAG TGGATGCTTC TGCCCAGCTG 
CTGCAGTGTG ATGTTCCAAT GAGCCCCCTG CAGGCTTCCA TACCTGATTA CACTCCCTCA 
GTGTTGGTGT CTGACCCGTG GGATGAGGAC CTGATAGATT CTTTACTCTC CAGCCTGAAG 
ACGCCCCTCA CCTCATACCC GAACCTCAGC CTCTGCACTC GAGGCCTGCC CACACTCACC 
CCCAAGATGT CCGTACAGAT GGGTGAGGAC TCTCTGCGGG TGGACTTTGT GTTGGGACAG 
GGTGCTTTCG CCACCGTTTA TCAGGCTACC AACCTTACAA CCTCACAAAA ATTCATCCTA 
AAGGTGCAGA AGCCGGCGAA CCCATGGGAG TTTTACATCA ACAGCCAGCT AAATGCTCGA 
GTGCTGCGGA AAGAGCGTCA CCTGTTCAAC CAGCTCCACT CCGCTCACCT GTTCACCAAT 
GGCAGTGTGC TTGTGGGGGA ACTGCACAAC TGCGGGACTC TGCTGAGTGT GGTGAATCTG 
TATAAGAGCC GCAGTGAGAA GGTGATGCCC CAGCCGCTGG TGCTGTACTT CAGCATGTGT 
ATCCTGCAGA TGGTGGAGCA GCTGCATCAA GCCAAAATCA TCCACGCCGA CATCAAACCT 
GACAACTTCC TGCTTGGAGA AAGGTTTCTG GAGAACGAGG ATTTGGATGC TGAGAGTCTG 
GATCACGGCT TGGTTCTGAT AGATTTTGGT CAAAGTATTG ATATGACCCT GTTCCCTGAG 
GGCACGGCCT TCACTGCCCG CTGCATGACT TCTGGCTTCC AGTGCACAGA GATGCTGAGT 
GGCCGGCCGT GGAACTACCA GACTGATTAC TTTGGCATTG CCGGCACCGT GCACTGCATG 
ATCTTCGGGT CATACATGCA AGTGAGAAAT GAGGGTGGAG TTTGGAAAAC GAATGGCATT 
TTTAAAAGGA ATCCTCACAG TGATCTATGG CAGGAGTTTT TCCATGTGCT GCTGAATGTT 
CCGGACTGTA ACTCTGCCTT ATGTTTGCAG AGTTTGCGTG AGCGTCTGTC TGTGGTGCTG 
CAGGAAAACT ACAGCAACAA ACTGCGCGGC CTTAAAAACC GACTGGTGGT GCAGATCCTG 
GAGAGCCGCG GGGCTCGCAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_007259885.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007259947.1 Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

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CloneID	OAs41539
Clone ID Related Accession (Same CDS sequence)	XM_007259885.2
Accession Version	XM_007259885.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749470.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007259885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CCACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCGTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGTGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACACATCTC ACGGAGGCTT TAATCATTCT 
CATGTCACTC CTAACACATC TCTGGGTCTG ATTTCGGCGA CTCCTTCACG TGCACTCCCC 
TCTCCCACCG TCAACACTCG GGAGGCTCTG GACGTGATCA TGGACATGTT CCAGGCTCCA 
ACGCTGCTGC AGGACTCCGT CTTCAATACT ACCAGCCAGG TGGCTGATGA CAGCTTTGAG 
AAAAACTGCA GAGTAAACGG TTTTGCCCCA TCTGTAGCGA ATCCACCTTC CTCAATGTCG 
TTCAGGATAT ACCAGGATGA AAATGTGAAG ATTGGAAGTG AGGAACAACA GGTGGAGAAG 
AGCAAACCAG CAGCTCGAGC CCTGATGGAG ATTCCGGTCT CCAAACCCAA TGTCACTCCT 
GTAGGTCCGG AGTCTCTGAC AGACGAGAGT GCGATGTGGG GTCCTCAATA TGCCTCCATA 
ACTGCCTGTC CAAATCATAC TCGTGATTTT GCCATGGCTG CCCACCTGGT ATCCACCCCC 
CTCCACCCCA CTGCCCCGTA CACCTGGGAC ACAGAGCTCA ATCAGGTAGA AAACACTGCC 
AGAGGATTTA GTGGGACAGA GGAAAACCTG TATCAGCGCC AGCCAGCCAA ACTGAGTCCA 
ATCCTGGAGC AGAGTCCTTC TGATGAGAAG CCGTCTGAAG TTCCTGAGTG CAGTCTGAGG 
GCTCAGGGCA CCATCGTAGG AGAGGGAGTG AGTCTTCCTC AGCAGAACCA GACGCTGACC 
CATGACCAGA CCAGCTGCAG CATCGGCAGG CAGACGCTGG TCCCCCTGGC TTTACATGAT 
CACACTGCCG AGGCCAGCAG AGCCCTCAAC TCCAGCACTA AACCTAGCTG GAGTGTCTAC 
CAGAGCCCAG AGAGAACCAG CCAGACAGGT TCTAACACAG AACCTGTGTC CAACAGCATG 
CTGTCTGTGA AACAGCAAAT ACCTCACCAG CTCAATTCAT CTAATCTACA GCTTAATTCC 
AGCTCTAGCA TCAAACCCAG CTGGAGCATC TACCAGAGCC CAGAGAAAAC CAGCCATACG 
GGCTCGAACA GAGGGCTCAA CTCAAGCAGC ATGCTGTCTG TAAGAGAGCC TCAACCGATC 
CAATCCAATG CAAATGGCCT ACAGATGAAC TCTCTATCTT GCTCCCTTGC TGAGGCCAGC 
AATGCCCTCA ACTCCAGTGC CAAACCTAAC TGGAGCATCT ACCAGAGCCC AGAGAAAACC 
AGCCAGACAG ATTGTAACAC AGAACCGACC TCTAACAGCA TACTGTCGGT AAAGCAGTAC 
CAAGCAGACC AGTTCAATTT ACCTAGCATG CAGGCTAACT CCAGCTCCAG CACTAAACCC 
AGCTGGACCA TCTACCAAAG CCCAGAGAAA TCCCACCAGG CTAGTTTTTG TGTGGAACCC 
AAACAACACT CAAACAGCAT GCACTTGGAA GCGGGAGAAC CCAAACAGGA CCAGTTCAAC 
TCAACTGGCT TGCAGGTTAA TCCAGACAGA ACCATGAACT CCACCACCCA AAGCCCAGAG 
AGAACCCAAA AGACTCAATT TAGTGGGGAC CTCCACTCAA CCAGCATGCA TTTAGTGGCA 
GAACCCAAAC AAGACCAGCT TAACTCAACA GCCATTCAGC TGAACTCCAG CTGCAGCACC 
AAACCCAGCT GGAGTATTTA CCAGAGCCCA GAGAGAACCA GTCAAATGGG TCACAGCAGG 
GAGTTCAAGG CAGACCAGCA GGTTAACTCA AACCAAGGGT TCAACTCTTC TAGCCTGCAT 
TTAATGGGAG AACTAAAACC AGACCAACTC AATTCCTCCA TCAATATGTG TTCTGGTCAG 
GGTGAGGCTG ATATTCTCTT GCCCAAGAAG TCTTTTCACC GGCGAAAATC CGAAAAACAG 
CTGAACTTCG AGAGCCTTCA GGTGAGCCGA CCTGTATCAC ACCAGTCCTG CACGCTGCCA 
GAGAGCCCAA AACCACAGCC CAGAATAATC CAGGATGTTC CCATGAGTCC AGACCCAATC 
CCAGGGTCCG ACTGGCTTCA GATGGACAGT CCAGTCCCGC TCTCTGAACC CGACCTGGAT 
GTGATGGTCA GGTCGATCAG ATCCCAGACA ATTCACAGAG AAACCCCCGG AGCTCCTGCT 
TCCAGGTCCA AACTCAGCTG GAGCGTGAAC CAGACCCCAG GGAGGCCATC TGAACTACAG 
TACGTCCCGC AGAGTCCTGA GCCCATGGAG GAATCAGATC CGCATCAGTT ATCAAAGCTG 
GAAGAGTCTG GACTTCTGCT CTCCAAGAGA TCCTTTAACA GGCGCAAATC GGATAAGAGG 
GTATTGGAGA AATCCCTCTT GTCGAGTCAG AGGCAGTCCT CCAACCAAAA CCAGGATGTT 
CCAATGAGTC CGGAACCAAT TTCGGGGCTG AACTGGCTCT GTCCAGAGAG TCCAGCCCGT 
GTGACTGAGT CTGATCTGGA CGTCATGATG ACACCTCAGC AAAAATCTAA ATCAGTGGAT 
GCTTCTGCCC AGCTGCTGCA GTGTGATGTT CCAATGAGCC CCCTGCAGGC TTCCATACCT 
GATTACACTC CCTCAGTGTT GGTGTCTGAC CCGTGGGATG AGGACCTGAT AGATTCTTTA 
CTCTCCAGCC TGAAGACGCC CCTCACCTCA TACCCGAACC TCAGCCTCTG CACTCGAGGC 
CTGCCCACAC TCACCCCCAA GATGTCCGTA CAGATGGGTG AGGACTCTCT GCGGGTGGAC 
TTTGTGTTGG GACAGGGTGC TTTCGCCACC GTTTATCAGG CTACCAACCT TACAACCTCA 
CAAAAATTCA TCCTAAAGGT GCAGAAGCCG GCGAACCCAT GGGAGTTTTA CATCAACAGC 
CAGCTAAATG CTCGAGTGCT GCGGAAAGAG CGTCACCTGT TCAACCAGCT CCACTCCGCT 
CACCTGTTCA CCAATGGCAG TGTGCTTGTG GGGGAACTGC ACAACTGCGG GACTCTGCTG 
AGTGTGGTGA ATCTGTATAA GAGCCGCAGT GAGAAGGTGA TGCCCCAGCC GCTGGTGCTG 
TACTTCAGCA TGTGTATCCT GCAGATGGTG GAGCAGCTGC ATCAAGCCAA AATCATCCAC 
GCCGACATCA AACCTGACAA CTTCCTGCTT GGAGAAAGGT TTCTGGAGAA CGAGGATTTG 
GATGCTGAGA GTCTGGATCA CGGCTTGGTT CTGATAGATT TTGGTCAAAG TATTGATATG 
ACCCTGTTCC CTGAGGGCAC GGCCTTCACT GCCCGCTGCA TGACTTCTGG CTTCCAGTGC 
ACAGAGATGC TGAGTGGCCG GCCGTGGAAC TACCAGACTG ATTACTTTGG CATTGCCGGC 
ACCGTGCACT GCATGATCTT CGGGTCATAC ATGCAAGTGA GAAATGAGGG TGGAGTTTGG 
AAAACGAATG GCATTTTTAA AAGGAATCCT CACAGTGATC TATGGCAGGA GTTTTTCCAT 
GTGCTGCTGA ATGTTCCGGA CTGTAACTCT GCCTTATGTT TGCGGAGTTT GCGTGAGCGT 
CTGTCTGTGG TGCTGCAGGA AAACTACAGC AACAAACTGC GCGGCCTTAA AAACCGACTG 
GTGGTGCAGA TCCTGGAGAG CCGCGGGGCT CGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007259947.1
CDS	152..5110
Translation	MDINSYLQQFEATVSSYVGEDPLDPWDKFVEFLECRLAAEERSNLCVVLERLVQTFLQDKRYRNDPRYINHCIKCAGYYGEPIKLYSYVHSLGVGARAAGLYVAWAQQFESKGLFTQAEQVYQRAIENQAEPMDTIQEQYRLFQSRMGKSQAASDHVRNPLQNSQLMNQNPRHRETLQPHCKEPDNSPLPADRTIRIISRSENNPMPRSSSSVESVPMYCVKELECEGSELSFEELRARRYFSKRQQLELLRKQEEVIWKHEEEEEEVIRMKKLLEELNSKLSTEQQETKHTFLQQELNESCSPLDRSVRPPLTPAMAEAAAPTSVTKEQAACSDIHRLQHAPECPLLNGRKSESHLLWQSTRVPASRLSLSLSAASQLAHSSALSAAVNRPLDLDTQAPVPLQLQDQRTASVCESQAERSLLESCSQRLAVFERDRSEKTEMDGGDANADTSHGGFNHSHVTPNTSLGLISATPSRALPSPTVNTREALDVIMDMFQAPTLLQDSVFNTTSQVADDSFEKNCRVNGFAPSVANPPSSMSFRIYQDENVKIGSEEQQVEKSKPAARALMEIPVSKPNVTPVGPESLTDESAMWGPQYASITACPNHTRDFAMAAHLVSTPLHPTAPYTWDTELNQVENTARGFSGTEENLYQRQPAKLSPILEQSPSDEKPSEVPECSLRAQGTIVGEGVSLPQQNQTLTHDQTSCSIGRQTLVPLALHDHTAEASRALNSSTKPSWSVYQSPERTSQTGSNTEPVSNSMLSVKQQIPHQLNSSNLQLNSSSSIKPSWSIYQSPEKTSHTGSNRGLNSSSMLSVREPQPIQSNANGLQMNSLSCSLAEASNALNSSAKPNWSIYQSPEKTSQTDCNTEPTSNSILSVKQYQADQFNLPSMQANSSSSTKPSWTIYQSPEKSHQASFCVEPKQHSNSMHLEAGEPKQDQFNSTGLQVNPDRTMNSTTQSPERTQKTQFSGDLHSTSMHLVAEPKQDQLNSTAIQLNSSCSTKPSWSIYQSPERTSQMGHSREFKADQQVNSNQGFNSSSLHLMGELKPDQLNSSINMCSGQGEADILLPKKSFHRRKSEKQLNFESLQVSRPVSHQSCTLPESPKPQPRIIQDVPMSPDPIPGSDWLQMDSPVPLSEPDLDVMVRSIRSQTIHRETPGAPASRSKLSWSVNQTPGRPSELQYVPQSPEPMEESDPHQLSKLEESGLLLSKRSFNRRKSDKRVLEKSLLSSQRQSSNQNQDVPMSPEPISGLNWLCPESPARVTESDLDVMMTPQQKSKSVDASAQLLQCDVPMSPLQASIPDYTPSVLVSDPWDEDLIDSLLSSLKTPLTSYPNLSLCTRGLPTLTPKMSVQMGEDSLRVDFVLGQGAFATVYQATNLTTSQKFILKVQKPANPWEFYINSQLNARVLRKERHLFNQLHSAHLFTNGSVLVGELHNCGTLLSVVNLYKSRSEKVMPQPLVLYFSMCILQMVEQLHQAKIIHADIKPDNFLLGERFLENEDLDAESLDHGLVLIDFGQSIDMTLFPEGTAFTARCMTSGFQCTEMLSGRPWNYQTDYFGIAGTVHCMIFGSYMQVRNEGGVWKTNGIFKRNPHSDLWQEFFHVLLNVPDCNSALCLRSLRERLSVVLQENYSNKLRGLKNRLVVQILESRGARR

Target ORF information:

RefSeq Version	XM_007259885.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007259885.2

ORF Insert Sequence:

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CCACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCGTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGTGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACACATCTC ACGGAGGCTT TAATCATTCT 
CATGTCACTC CTAACACATC TCTGGGTCTG ATTTCGGCGA CTCCTTCACG TGCACTCCCC 
TCTCCCACCG TCAACACTCG GGAGGCTCTG GACGTGATCA TGGACATGTT CCAGGCTCCA 
ACGCTGCTGC AGGACTCCGT CTTCAATACT ACCAGCCAGG TGGCTGATGA CAGCTTTGAG 
AAAAACTGCA GAGTAAACGG TTTTGCCCCA TCTGTAGCGA ATCCACCTTC CTCAATGTCG 
TTCAGGATAT ACCAGGATGA AAATGTGAAG ATTGGAAGTG AGGAACAACA GGTGGAGAAG 
AGCAAACCAG CAGCTCGAGC CCTGATGGAG ATTCCGGTCT CCAAACCCAA TGTCACTCCT 
GTAGGTCCGG AGTCTCTGAC AGACGAGAGT GCGATGTGGG GTCCTCAATA TGCCTCCATA 
ACTGCCTGTC CAAATCATAC TCGTGATTTT GCCATGGCTG CCCACCTGGT ATCCACCCCC 
CTCCACCCCA CTGCCCCGTA CACCTGGGAC ACAGAGCTCA ATCAGGTAGA AAACACTGCC 
AGAGGATTTA GTGGGACAGA GGAAAACCTG TATCAGCGCC AGCCAGCCAA ACTGAGTCCA 
ATCCTGGAGC AGAGTCCTTC TGATGAGAAG CCGTCTGAAG TTCCTGAGTG CAGTCTGAGG 
GCTCAGGGCA CCATCGTAGG AGAGGGAGTG AGTCTTCCTC AGCAGAACCA GACGCTGACC 
CATGACCAGA CCAGCTGCAG CATCGGCAGG CAGACGCTGG TCCCCCTGGC TTTACATGAT 
CACACTGCCG AGGCCAGCAG AGCCCTCAAC TCCAGCACTA AACCTAGCTG GAGTGTCTAC 
CAGAGCCCAG AGAGAACCAG CCAGACAGGT TCTAACACAG AACCTGTGTC CAACAGCATG 
CTGTCTGTGA AACAGCAAAT ACCTCACCAG CTCAATTCAT CTAATCTACA GCTTAATTCC 
AGCTCTAGCA TCAAACCCAG CTGGAGCATC TACCAGAGCC CAGAGAAAAC CAGCCATACG 
GGCTCGAACA GAGGGCTCAA CTCAAGCAGC ATGCTGTCTG TAAGAGAGCC TCAACCGATC 
CAATCCAATG CAAATGGCCT ACAGATGAAC TCTCTATCTT GCTCCCTTGC TGAGGCCAGC 
AATGCCCTCA ACTCCAGTGC CAAACCTAAC TGGAGCATCT ACCAGAGCCC AGAGAAAACC 
AGCCAGACAG ATTGTAACAC AGAACCGACC TCTAACAGCA TACTGTCGGT AAAGCAGTAC 
CAAGCAGACC AGTTCAATTT ACCTAGCATG CAGGCTAACT CCAGCTCCAG CACTAAACCC 
AGCTGGACCA TCTACCAAAG CCCAGAGAAA TCCCACCAGG CTAGTTTTTG TGTGGAACCC 
AAACAACACT CAAACAGCAT GCACTTGGAA GCGGGAGAAC CCAAACAGGA CCAGTTCAAC 
TCAACTGGCT TGCAGGTTAA TCCAGACAGA ACCATGAACT CCACCACCCA AAGCCCAGAG 
AGAACCCAAA AGACTCAATT TAGTGGGGAC CTCCACTCAA CCAGCATGCA TTTAGTGGCA 
GAACCCAAAC AAGACCAGCT TAACTCAACA GCCATTCAGC TGAACTCCAG CTGCAGCACC 
AAACCCAGCT GGAGTATTTA CCAGAGCCCA GAGAGAACCA GTCAAATGGG TCACAGCAGG 
GAGTTCAAGG CAGACCAGCA GGTTAACTCA AACCAAGGGT TCAACTCTTC TAGCCTGCAT 
TTAATGGGAG AACTAAAACC AGACCAACTC AATTCCTCCA TCAATATGTG TTCTGGTCAG 
GGTGAGGCTG ATATTCTCTT GCCCAAGAAG TCTTTTCACC GGCGAAAATC CGAAAAACAG 
CTGAACTTCG AGAGCCTTCA GGTGAGCCGA CCTGTATCAC ACCAGTCCTG CACGCTGCCA 
GAGAGCCCAA AACCACAGCC CAGAATAATC CAGGATGTTC CCATGAGTCC AGACCCAATC 
CCAGGGTCCG ACTGGCTTCA GATGGACAGT CCAGTCCCGC TCTCTGAACC CGACCTGGAT 
GTGATGGTCA GGTCGATCAG ATCCCAGACA ATTCACAGAG AAACCCCCGG AGCTCCTGCT 
TCCAGGTCCA AACTCAGCTG GAGCGTGAAC CAGACCCCAG GGAGGCCATC TGAACTACAG 
TACGTCCCGC AGAGTCCTGA GCCCATGGAG GAATCAGATC CGCATCAGTT ATCAAAGCTG 
GAAGAGTCTG GACTTCTGCT CTCCAAGAGA TCCTTTAACA GGCGCAAATC GGATAAGAGG 
GTATTGGAGA AATCCCTCTT GTCGAGTCAG AGGCAGTCCT CCAACCAAAA CCAGGATGTT 
CCAATGAGTC CGGAACCAAT TTCGGGGCTG AACTGGCTCT GTCCAGAGAG TCCAGCCCGT 
GTGACTGAGT CTGATCTGGA CGTCATGATG ACACCTCAGC AAAAATCTAA ATCAGTGGAT 
GCTTCTGCCC AGCTGCTGCA GTGTGATGTT CCAATGAGCC CCCTGCAGGC TTCCATACCT 
GATTACACTC CCTCAGTGTT GGTGTCTGAC CCGTGGGATG AGGACCTGAT AGATTCTTTA 
CTCTCCAGCC TGAAGACGCC CCTCACCTCA TACCCGAACC TCAGCCTCTG CACTCGAGGC 
CTGCCCACAC TCACCCCCAA GATGTCCGTA CAGATGGGTG AGGACTCTCT GCGGGTGGAC 
TTTGTGTTGG GACAGGGTGC TTTCGCCACC GTTTATCAGG CTACCAACCT TACAACCTCA 
CAAAAATTCA TCCTAAAGGT GCAGAAGCCG GCGAACCCAT GGGAGTTTTA CATCAACAGC 
CAGCTAAATG CTCGAGTGCT GCGGAAAGAG CGTCACCTGT TCAACCAGCT CCACTCCGCT 
CACCTGTTCA CCAATGGCAG TGTGCTTGTG GGGGAACTGC ACAACTGCGG GACTCTGCTG 
AGTGTGGTGA ATCTGTATAA GAGCCGCAGT GAGAAGGTGA TGCCCCAGCC GCTGGTGCTG 
TACTTCAGCA TGTGTATCCT GCAGATGGTG GAGCAGCTGC ATCAAGCCAA AATCATCCAC 
GCCGACATCA AACCTGACAA CTTCCTGCTT GGAGAAAGGT TTCTGGAGAA CGAGGATTTG 
GATGCTGAGA GTCTGGATCA CGGCTTGGTT CTGATAGATT TTGGTCAAAG TATTGATATG 
ACCCTGTTCC CTGAGGGCAC GGCCTTCACT GCCCGCTGCA TGACTTCTGG CTTCCAGTGC 
ACAGAGATGC TGAGTGGCCG GCCGTGGAAC TACCAGACTG ATTACTTTGG CATTGCCGGC 
ACCGTGCACT GCATGATCTT CGGGTCATAC ATGCAAGTGA GAAATGAGGG TGGAGTTTGG 
AAAACGAATG GCATTTTTAA AAGGAATCCT CACAGTGATC TATGGCAGGA GTTTTTCCAT 
GTGCTGCTGA ATGTTCCGGA CTGTAACTCT GCCTTATGTT TGCGGAGTTT GCGTGAGCGT 
CTGTCTGTGG TGCTGCAGGA AAACTACAGC AACAAACTGC GCGGCCTTAA AAACCGACTG 
GTGGTGCAGA TCCTGGAGAG CCGCGGGGCT CGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_015608507.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015463993.1 Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

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CloneID	OAs41540
Clone ID Related Accession (Same CDS sequence)	XM_015608507.1
Accession Version	XM_015608507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749470.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CCACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCGTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGTGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACACATCTC ACGGAGGCTT TAATCATTCT 
CATGTCACTC CTAACACATC TCTGGGTCTG ATTTCGGCGA CTCCTTCACG TGCACTCCCC 
TCTCCCACCG TCAACACTCG GGAGGCTCTG GACGTGATCA TGGACATGTT CCAGGCTCCA 
ACGCTGCTGC AGGACTCCGT CTTCAATACT ACCAGCCAGG TGGCTGATGA CAGCTTTGAG 
AAAAACTGCA GAGTAAACGG TTTTGCCCCA TCTGTAGCGA ATCCACCTTC CTCAATGTCG 
TTCAGGATAT ACCAGGATGA AAATGTGAAG ATTGGAAGTG AGGAACAACA GGTGGAGAAG 
AGCAAACCAG CAGCTCGAGC CCTGATGGAG ATTCCGGTCT CCAAACCCAA TGTCACTCCT 
GTAGGTCCGG AGTCTCTGAC AGACGAGAGT GCGATGTGGG GTCCTCAATA TGCCTCCATA 
ACTGCCTGTC CAAATCATAC TCGTGATTTT GCCATGGCTG CCCACCTGGT ATCCACCCCC 
CTCCACCCCA CTGCCCCGTA CACCTGGGAC ACAGAGCTCA ATCAGGAAAA CACTGCCAGA 
GGATTTAGTG GGACAGAGGA AAACCTGTAT CAGCGCCAGC CAGCCAAACT GAGTCCAATC 
CTGGAGCAGA GTCCTTCTGA TGAGAAGCCG TCTGAAGTTC CTGAGTGCAG TCTGAGGGCT 
CAGGGCACCA TCGTAGGAGA GGGAGTGAGT CTTCCTCAGC AGAACCAGAC GCTGACCCAT 
GACCAGACCA GCTGCAGCAT CGGCAGGCAG ACGCTGGTCC CCCTGGCTTT ACATGATCAC 
ACTGCCGAGG CCAGCAGAGC CCTCAACTCC AGCACTAAAC CTAGCTGGAG TGTCTACCAG 
AGCCCAGAGA GAACCAGCCA GACAGGTTCT AACACAGAAC CTGTGTCCAA CAGCATGCTG 
TCTGTGAAAC AGCAAATACC TCACCAGCTC AATTCATCTA ATCTACAGCT TAATTCCAGC 
TCTAGCATCA AACCCAGCTG GAGCATCTAC CAGAGCCCAG AGAAAACCAG CCATACGGGC 
TCGAACAGAG GGCTCAACTC AAGCAGCATG CTGTCTGTAA GAGAGCCTCA ACCGATCCAA 
TCCAATGCAA ATGGCCTACA GATGAACTCT CTATCTTGCT CCCTTGCTGA GGCCAGCAAT 
GCCCTCAACT CCAGTGCCAA ACCTAACTGG AGCATCTACC AGAGCCCAGA GAAAACCAGC 
CAGACAGATT GTAACACAGA ACCGACCTCT AACAGCATAC TGTCGGTAAA GCAGTACCAA 
GCAGACCAGT TCAATTTACC TAGCATGCAG GCTAACTCCA GCTCCAGCAC TAAACCCAGC 
TGGACCATCT ACCAAAGCCC AGAGAAATCC CACCAGGCTA GTTTTTGTGT GGAACCCAAA 
CAACACTCAA ACAGCATGCA CTTGGAAGCG GGAGAACCCA AACAGGACCA GTTCAACTCA 
ACTGGCTTGC AGGTTAATCC AGACAGAACC ATGAACTCCA CCACCCAAAG CCCAGAGAGA 
ACCCAAAAGA CTCAATTTAG TGGGGACCTC CACTCAACCA GCATGCATTT AGTGGCAGAA 
CCCAAACAAG ACCAGCTTAA CTCAACAGCC ATTCAGCTGA ACTCCAGCTG CAGCACCAAA 
CCCAGCTGGA GTATTTACCA GAGCCCAGAG AGAACCAGTC AAATGGGTCA CAGCAGGGAG 
TTCAAGGCAG ACCAGCAGGT TAACTCAAAC CAAGGGTTCA ACTCTTCTAG CCTGCATTTA 
ATGGGAGAAC TAAAACCAGA CCAACTCAAT TCCTCCATCA ATATGTGTTC TGGTCAGGGT 
GAGGCTGATA TTCTCTTGCC CAAGAAGTCT TTTCACCGGC GAAAATCCGA AAAACAGCTG 
AACTTCGAGA GCCTTCAGGT GAGCCGACCT GTATCACACC AGTCCTGCAC GCTGCCAGAG 
AGCCCAAAAC CACAGCCCAG AATAATCCAG GATGTTCCCA TGAGTCCAGA CCCAATCCCA 
GGGTCCGACT GGCTTCAGAT GGACAGTCCA GTCCCGCTCT CTGAACCCGA CCTGGATGTG 
ATGGTCAGGT CGATCAGATC CCAGACAATT CACAGAGAAA CCCCCGGAGC TCCTGCTTCC 
AGGTCCAAAC TCAGCTGGAG CGTGAACCAG ACCCCAGGGA GGCCATCTGA ACTACAGTAC 
GTCCCGCAGA GTCCTGAGCC CATGGAGGAA TCAGATCCGC ATCAGTTATC AAAGCTGGAA 
GAGTCTGGAC TTCTGCTCTC CAAGAGATCC TTTAACAGGC GCAAATCGGA TAAGAGGGTA 
TTGGAGAAAT CCCTCTTGTC GAGTCAGAGG CAGTCCTCCA ACCAAAACCA GGATGTTCCA 
ATGAGTCCGG AACCAATTTC GGGGCTGAAC TGGCTCTGTC CAGAGAGTCC AGCCCGTGTG 
ACTGAGTCTG ATCTGGACGT CATGATGACA CCTCAGCAAA AATCTAAATC AGTGGATGCT 
TCTGCCCAGC TGCTGCAGTG TGATGTTCCA ATGAGCCCCC TGCAGGCTTC CATACCTGAT 
TACACTCCCT CAGTGTTGGT GTCTGACCCG TGGGATGAGG ACCTGATAGA TTCTTTACTC 
TCCAGCCTGA AGACGCCCCT CACCTCATAC CCGAACCTCA GCCTCTGCAC TCGAGGCCTG 
CCCACACTCA CCCCCAAGAT GTCCGTACAG ATGGGTGAGG ACTCTCTGCG GGTGGACTTT 
GTGTTGGGAC AGGGTGCTTT CGCCACCGTT TATCAGGCTA CCAACCTTAC AACCTCACAA 
AAATTCATCC TAAAGGTGCA GAAGCCGGCG AACCCATGGG AGTTTTACAT CAACAGCCAG 
CTAAATGCTC GAGTGCTGCG GAAAGAGCGT CACCTGTTCA ACCAGCTCCA CTCCGCTCAC 
CTGTTCACCA ATGGCAGTGT GCTTGTGGGG GAACTGCACA ACTGCGGGAC TCTGCTGAGT 
GTGGTGAATC TGTATAAGAG CCGCAGTGAG AAGGTGATGC CCCAGCCGCT GGTGCTGTAC 
TTCAGCATGT GTATCCTGCA GATGGTGGAG CAGCTGCATC AAGCCAAAAT CATCCACGCC 
GACATCAAAC CTGACAACTT CCTGCTTGGA GAAAGGTTTC TGGAGAACGA GGATTTGGAT 
GCTGAGAGTC TGGATCACGG CTTGGTTCTG ATAGATTTTG GTCAAAGTAT TGATATGACC 
CTGTTCCCTG AGGGCACGGC CTTCACTGCC CGCTGCATGA CTTCTGGCTT CCAGTGCACA 
GAGATGCTGA GTGGCCGGCC GTGGAACTAC CAGACTGATT ACTTTGGCAT TGCCGGCACC 
GTGCACTGCA TGATCTTCGG GTCATACATG CAAGTGAGAA ATGAGGGTGG AGTTTGGAAA 
ACGAATGGCA TTTTTAAAAG GAATCCTCAC AGTGATCTAT GGCAGGAGTT TTTCCATGTG 
CTGCTGAATG TTCCGGACTG TAACTCTGCC TTATGTTTGC GGAGTTTGCG TGAGCGTCTG 
TCTGTGGTGC TGCAGGAAAA CTACAGCAAC AAACTGCGCG GCCTTAAAAA CCGACTGGTG 
GTGCAGATCC TGGAGAGCCG CGGGGCTCGC AGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015463993.1
CDS	152..5107
Translation	MDINSYLQQFEATVSSYVGEDPLDPWDKFVEFLECRLAAEERSNLCVVLERLVQTFLQDKRYRNDPRYINHCIKCAGYYGEPIKLYSYVHSLGVGARAAGLYVAWAQQFESKGLFTQAEQVYQRAIENQAEPMDTIQEQYRLFQSRMGKSQAASDHVRNPLQNSQLMNQNPRHRETLQPHCKEPDNSPLPADRTIRIISRSENNPMPRSSSSVESVPMYCVKELECEGSELSFEELRARRYFSKRQQLELLRKQEEVIWKHEEEEEEVIRMKKLLEELNSKLSTEQQETKHTFLQQELNESCSPLDRSVRPPLTPAMAEAAAPTSVTKEQAACSDIHRLQHAPECPLLNGRKSESHLLWQSTRVPASRLSLSLSAASQLAHSSALSAAVNRPLDLDTQAPVPLQLQDQRTASVCESQAERSLLESCSQRLAVFERDRSEKTEMDGGDANADTSHGGFNHSHVTPNTSLGLISATPSRALPSPTVNTREALDVIMDMFQAPTLLQDSVFNTTSQVADDSFEKNCRVNGFAPSVANPPSSMSFRIYQDENVKIGSEEQQVEKSKPAARALMEIPVSKPNVTPVGPESLTDESAMWGPQYASITACPNHTRDFAMAAHLVSTPLHPTAPYTWDTELNQENTARGFSGTEENLYQRQPAKLSPILEQSPSDEKPSEVPECSLRAQGTIVGEGVSLPQQNQTLTHDQTSCSIGRQTLVPLALHDHTAEASRALNSSTKPSWSVYQSPERTSQTGSNTEPVSNSMLSVKQQIPHQLNSSNLQLNSSSSIKPSWSIYQSPEKTSHTGSNRGLNSSSMLSVREPQPIQSNANGLQMNSLSCSLAEASNALNSSAKPNWSIYQSPEKTSQTDCNTEPTSNSILSVKQYQADQFNLPSMQANSSSSTKPSWTIYQSPEKSHQASFCVEPKQHSNSMHLEAGEPKQDQFNSTGLQVNPDRTMNSTTQSPERTQKTQFSGDLHSTSMHLVAEPKQDQLNSTAIQLNSSCSTKPSWSIYQSPERTSQMGHSREFKADQQVNSNQGFNSSSLHLMGELKPDQLNSSINMCSGQGEADILLPKKSFHRRKSEKQLNFESLQVSRPVSHQSCTLPESPKPQPRIIQDVPMSPDPIPGSDWLQMDSPVPLSEPDLDVMVRSIRSQTIHRETPGAPASRSKLSWSVNQTPGRPSELQYVPQSPEPMEESDPHQLSKLEESGLLLSKRSFNRRKSDKRVLEKSLLSSQRQSSNQNQDVPMSPEPISGLNWLCPESPARVTESDLDVMMTPQQKSKSVDASAQLLQCDVPMSPLQASIPDYTPSVLVSDPWDEDLIDSLLSSLKTPLTSYPNLSLCTRGLPTLTPKMSVQMGEDSLRVDFVLGQGAFATVYQATNLTTSQKFILKVQKPANPWEFYINSQLNARVLRKERHLFNQLHSAHLFTNGSVLVGELHNCGTLLSVVNLYKSRSEKVMPQPLVLYFSMCILQMVEQLHQAKIIHADIKPDNFLLGERFLENEDLDAESLDHGLVLIDFGQSIDMTLFPEGTAFTARCMTSGFQCTEMLSGRPWNYQTDYFGIAGTVHCMIFGSYMQVRNEGGVWKTNGIFKRNPHSDLWQEFFHVLLNVPDCNSALCLRSLRERLSVVLQENYSNKLRGLKNRLVVQILESRGARR

Target ORF information:

RefSeq Version	XM_015608507.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015608507.1

ORF Insert Sequence:

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CCACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCGTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGTGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACACATCTC ACGGAGGCTT TAATCATTCT 
CATGTCACTC CTAACACATC TCTGGGTCTG ATTTCGGCGA CTCCTTCACG TGCACTCCCC 
TCTCCCACCG TCAACACTCG GGAGGCTCTG GACGTGATCA TGGACATGTT CCAGGCTCCA 
ACGCTGCTGC AGGACTCCGT CTTCAATACT ACCAGCCAGG TGGCTGATGA CAGCTTTGAG 
AAAAACTGCA GAGTAAACGG TTTTGCCCCA TCTGTAGCGA ATCCACCTTC CTCAATGTCG 
TTCAGGATAT ACCAGGATGA AAATGTGAAG ATTGGAAGTG AGGAACAACA GGTGGAGAAG 
AGCAAACCAG CAGCTCGAGC CCTGATGGAG ATTCCGGTCT CCAAACCCAA TGTCACTCCT 
GTAGGTCCGG AGTCTCTGAC AGACGAGAGT GCGATGTGGG GTCCTCAATA TGCCTCCATA 
ACTGCCTGTC CAAATCATAC TCGTGATTTT GCCATGGCTG CCCACCTGGT ATCCACCCCC 
CTCCACCCCA CTGCCCCGTA CACCTGGGAC ACAGAGCTCA ATCAGGAAAA CACTGCCAGA 
GGATTTAGTG GGACAGAGGA AAACCTGTAT CAGCGCCAGC CAGCCAAACT GAGTCCAATC 
CTGGAGCAGA GTCCTTCTGA TGAGAAGCCG TCTGAAGTTC CTGAGTGCAG TCTGAGGGCT 
CAGGGCACCA TCGTAGGAGA GGGAGTGAGT CTTCCTCAGC AGAACCAGAC GCTGACCCAT 
GACCAGACCA GCTGCAGCAT CGGCAGGCAG ACGCTGGTCC CCCTGGCTTT ACATGATCAC 
ACTGCCGAGG CCAGCAGAGC CCTCAACTCC AGCACTAAAC CTAGCTGGAG TGTCTACCAG 
AGCCCAGAGA GAACCAGCCA GACAGGTTCT AACACAGAAC CTGTGTCCAA CAGCATGCTG 
TCTGTGAAAC AGCAAATACC TCACCAGCTC AATTCATCTA ATCTACAGCT TAATTCCAGC 
TCTAGCATCA AACCCAGCTG GAGCATCTAC CAGAGCCCAG AGAAAACCAG CCATACGGGC 
TCGAACAGAG GGCTCAACTC AAGCAGCATG CTGTCTGTAA GAGAGCCTCA ACCGATCCAA 
TCCAATGCAA ATGGCCTACA GATGAACTCT CTATCTTGCT CCCTTGCTGA GGCCAGCAAT 
GCCCTCAACT CCAGTGCCAA ACCTAACTGG AGCATCTACC AGAGCCCAGA GAAAACCAGC 
CAGACAGATT GTAACACAGA ACCGACCTCT AACAGCATAC TGTCGGTAAA GCAGTACCAA 
GCAGACCAGT TCAATTTACC TAGCATGCAG GCTAACTCCA GCTCCAGCAC TAAACCCAGC 
TGGACCATCT ACCAAAGCCC AGAGAAATCC CACCAGGCTA GTTTTTGTGT GGAACCCAAA 
CAACACTCAA ACAGCATGCA CTTGGAAGCG GGAGAACCCA AACAGGACCA GTTCAACTCA 
ACTGGCTTGC AGGTTAATCC AGACAGAACC ATGAACTCCA CCACCCAAAG CCCAGAGAGA 
ACCCAAAAGA CTCAATTTAG TGGGGACCTC CACTCAACCA GCATGCATTT AGTGGCAGAA 
CCCAAACAAG ACCAGCTTAA CTCAACAGCC ATTCAGCTGA ACTCCAGCTG CAGCACCAAA 
CCCAGCTGGA GTATTTACCA GAGCCCAGAG AGAACCAGTC AAATGGGTCA CAGCAGGGAG 
TTCAAGGCAG ACCAGCAGGT TAACTCAAAC CAAGGGTTCA ACTCTTCTAG CCTGCATTTA 
ATGGGAGAAC TAAAACCAGA CCAACTCAAT TCCTCCATCA ATATGTGTTC TGGTCAGGGT 
GAGGCTGATA TTCTCTTGCC CAAGAAGTCT TTTCACCGGC GAAAATCCGA AAAACAGCTG 
AACTTCGAGA GCCTTCAGGT GAGCCGACCT GTATCACACC AGTCCTGCAC GCTGCCAGAG 
AGCCCAAAAC CACAGCCCAG AATAATCCAG GATGTTCCCA TGAGTCCAGA CCCAATCCCA 
GGGTCCGACT GGCTTCAGAT GGACAGTCCA GTCCCGCTCT CTGAACCCGA CCTGGATGTG 
ATGGTCAGGT CGATCAGATC CCAGACAATT CACAGAGAAA CCCCCGGAGC TCCTGCTTCC 
AGGTCCAAAC TCAGCTGGAG CGTGAACCAG ACCCCAGGGA GGCCATCTGA ACTACAGTAC 
GTCCCGCAGA GTCCTGAGCC CATGGAGGAA TCAGATCCGC ATCAGTTATC AAAGCTGGAA 
GAGTCTGGAC TTCTGCTCTC CAAGAGATCC TTTAACAGGC GCAAATCGGA TAAGAGGGTA 
TTGGAGAAAT CCCTCTTGTC GAGTCAGAGG CAGTCCTCCA ACCAAAACCA GGATGTTCCA 
ATGAGTCCGG AACCAATTTC GGGGCTGAAC TGGCTCTGTC CAGAGAGTCC AGCCCGTGTG 
ACTGAGTCTG ATCTGGACGT CATGATGACA CCTCAGCAAA AATCTAAATC AGTGGATGCT 
TCTGCCCAGC TGCTGCAGTG TGATGTTCCA ATGAGCCCCC TGCAGGCTTC CATACCTGAT 
TACACTCCCT CAGTGTTGGT GTCTGACCCG TGGGATGAGG ACCTGATAGA TTCTTTACTC 
TCCAGCCTGA AGACGCCCCT CACCTCATAC CCGAACCTCA GCCTCTGCAC TCGAGGCCTG 
CCCACACTCA CCCCCAAGAT GTCCGTACAG ATGGGTGAGG ACTCTCTGCG GGTGGACTTT 
GTGTTGGGAC AGGGTGCTTT CGCCACCGTT TATCAGGCTA CCAACCTTAC AACCTCACAA 
AAATTCATCC TAAAGGTGCA GAAGCCGGCG AACCCATGGG AGTTTTACAT CAACAGCCAG 
CTAAATGCTC GAGTGCTGCG GAAAGAGCGT CACCTGTTCA ACCAGCTCCA CTCCGCTCAC 
CTGTTCACCA ATGGCAGTGT GCTTGTGGGG GAACTGCACA ACTGCGGGAC TCTGCTGAGT 
GTGGTGAATC TGTATAAGAG CCGCAGTGAG AAGGTGATGC CCCAGCCGCT GGTGCTGTAC 
TTCAGCATGT GTATCCTGCA GATGGTGGAG CAGCTGCATC AAGCCAAAAT CATCCACGCC 
GACATCAAAC CTGACAACTT CCTGCTTGGA GAAAGGTTTC TGGAGAACGA GGATTTGGAT 
GCTGAGAGTC TGGATCACGG CTTGGTTCTG ATAGATTTTG GTCAAAGTAT TGATATGACC 
CTGTTCCCTG AGGGCACGGC CTTCACTGCC CGCTGCATGA CTTCTGGCTT CCAGTGCACA 
GAGATGCTGA GTGGCCGGCC GTGGAACTAC CAGACTGATT ACTTTGGCAT TGCCGGCACC 
GTGCACTGCA TGATCTTCGG GTCATACATG CAAGTGAGAA ATGAGGGTGG AGTTTGGAAA 
ACGAATGGCA TTTTTAAAAG GAATCCTCAC AGTGATCTAT GGCAGGAGTT TTTCCATGTG 
CTGCTGAATG TTCCGGACTG TAACTCTGCC TTATGTTTGC GGAGTTTGCG TGAGCGTCTG 
TCTGTGGTGC TGCAGGAAAA CTACAGCAAC AAACTGCGCG GCCTTAAAAA CCGACTGGTG 
GTGCAGATCC TGGAGAGCCG CGGGGCTCGC AGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_007259886.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007259948.1 Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAs41541
Clone ID Related Accession (Same CDS sequence)	XM_007259886.2
Accession Version	XM_007259886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749470.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007259886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CCACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCGTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGTGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACAACGTGA TCATGGACAT GTTCCAGGCT 
CCAACGCTGC TGCAGGACTC CGTCTTCAAT ACTACCAGCC AGGTGGCTGA TGACAGCTTT 
GAGAAAAACT GCAGAGTAAA CGGTTTTGCC CCATCTGTAG CGAATCCACC TTCCTCAATG 
TCGTTCAGGA TATACCAGGA TGAAAATGTG AAGATTGGAA GTGAGGAACA ACAGGTGGAG 
AAGAGCAAAC CAGCAGCTCG AGCCCTGATG GAGATTCCGG TCTCCAAACC CAATGTCACT 
CCTGTAGGTC CGGAGTCTCT GACAGACGAG AGTGCGATGT GGGGTCCTCA ATATGCCTCC 
ATAACTGCCT GTCCAAATCA TACTCGTGAT TTTGCCATGG CTGCCCACCT GGTATCCACC 
CCCCTCCACC CCACTGCCCC GTACACCTGG GACACAGAGC TCAATCAGGT AGAAAACACT 
GCCAGAGGAT TTAGTGGGAC AGAGGAAAAC CTGTATCAGC GCCAGCCAGC CAAACTGAGT 
CCAATCCTGG AGCAGAGTCC TTCTGATGAG AAGCCGTCTG AAGTTCCTGA GTGCAGTCTG 
AGGGCTCAGG GCACCATCGT AGGAGAGGGA GTGAGTCTTC CTCAGCAGAA CCAGACGCTG 
ACCCATGACC AGACCAGCTG CAGCATCGGC AGGCAGACGC TGGTCCCCCT GGCTTTACAT 
GATCACACTG CCGAGGCCAG CAGAGCCCTC AACTCCAGCA CTAAACCTAG CTGGAGTGTC 
TACCAGAGCC CAGAGAGAAC CAGCCAGACA GGTTCTAACA CAGAACCTGT GTCCAACAGC 
ATGCTGTCTG TGAAACAGCA AATACCTCAC CAGCTCAATT CATCTAATCT ACAGCTTAAT 
TCCAGCTCTA GCATCAAACC CAGCTGGAGC ATCTACCAGA GCCCAGAGAA AACCAGCCAT 
ACGGGCTCGA ACAGAGGGCT CAACTCAAGC AGCATGCTGT CTGTAAGAGA GCCTCAACCG 
ATCCAATCCA ATGCAAATGG CCTACAGATG AACTCTCTAT CTTGCTCCCT TGCTGAGGCC 
AGCAATGCCC TCAACTCCAG TGCCAAACCT AACTGGAGCA TCTACCAGAG CCCAGAGAAA 
ACCAGCCAGA CAGATTGTAA CACAGAACCG ACCTCTAACA GCATACTGTC GGTAAAGCAG 
TACCAAGCAG ACCAGTTCAA TTTACCTAGC ATGCAGGCTA ACTCCAGCTC CAGCACTAAA 
CCCAGCTGGA CCATCTACCA AAGCCCAGAG AAATCCCACC AGGCTAGTTT TTGTGTGGAA 
CCCAAACAAC ACTCAAACAG CATGCACTTG GAAGCGGGAG AACCCAAACA GGACCAGTTC 
AACTCAACTG GCTTGCAGGT TAATCCAGAC AGAACCATGA ACTCCACCAC CCAAAGCCCA 
GAGAGAACCC AAAAGACTCA ATTTAGTGGG GACCTCCACT CAACCAGCAT GCATTTAGTG 
GCAGAACCCA AACAAGACCA GCTTAACTCA ACAGCCATTC AGCTGAACTC CAGCTGCAGC 
ACCAAACCCA GCTGGAGTAT TTACCAGAGC CCAGAGAGAA CCAGTCAAAT GGGTCACAGC 
AGGGAGTTCA AGGCAGACCA GCAGGTTAAC TCAAACCAAG GGTTCAACTC TTCTAGCCTG 
CATTTAATGG GAGAACTAAA ACCAGACCAA CTCAATTCCT CCATCAATAT GTGTTCTGGT 
CAGGGTGAGG CTGATATTCT CTTGCCCAAG AAGTCTTTTC ACCGGCGAAA ATCCGAAAAA 
CAGCTGAACT TCGAGAGCCT TCAGGTGAGC CGACCTGTAT CACACCAGTC CTGCACGCTG 
CCAGAGAGCC CAAAACCACA GCCCAGAATA ATCCAGGATG TTCCCATGAG TCCAGACCCA 
ATCCCAGGGT CCGACTGGCT TCAGATGGAC AGTCCAGTCC CGCTCTCTGA ACCCGACCTG 
GATGTGATGG TCAGGTCGAT CAGATCCCAG ACAATTCACA GAGAAACCCC CGGAGCTCCT 
GCTTCCAGGT CCAAACTCAG CTGGAGCGTG AACCAGACCC CAGGGAGGCC ATCTGAACTA 
CAGTACGTCC CGCAGAGTCC TGAGCCCATG GAGGAATCAG ATCCGCATCA GTTATCAAAG 
CTGGAAGAGT CTGGACTTCT GCTCTCCAAG AGATCCTTTA ACAGGCGCAA ATCGGATAAG 
AGGGTATTGG AGAAATCCCT CTTGTCGAGT CAGAGGCAGT CCTCCAACCA AAACCAGGAT 
GTTCCAATGA GTCCGGAACC AATTTCGGGG CTGAACTGGC TCTGTCCAGA GAGTCCAGCC 
CGTGTGACTG AGTCTGATCT GGACGTCATG ATGACACCTC AGCAAAAATC TAAATCAGTG 
GATGCTTCTG CCCAGCTGCT GCAGTGTGAT GTTCCAATGA GCCCCCTGCA GGCTTCCATA 
CCTGATTACA CTCCCTCAGT GTTGGTGTCT GACCCGTGGG ATGAGGACCT GATAGATTCT 
TTACTCTCCA GCCTGAAGAC GCCCCTCACC TCATACCCGA ACCTCAGCCT CTGCACTCGA 
GGCCTGCCCA CACTCACCCC CAAGATGTCC GTACAGATGG GTGAGGACTC TCTGCGGGTG 
GACTTTGTGT TGGGACAGGG TGCTTTCGCC ACCGTTTATC AGGCTACCAA CCTTACAACC 
TCACAAAAAT TCATCCTAAA GGTGCAGAAG CCGGCGAACC CATGGGAGTT TTACATCAAC 
AGCCAGCTAA ATGCTCGAGT GCTGCGGAAA GAGCGTCACC TGTTCAACCA GCTCCACTCC 
GCTCACCTGT TCACCAATGG CAGTGTGCTT GTGGGGGAAC TGCACAACTG CGGGACTCTG 
CTGAGTGTGG TGAATCTGTA TAAGAGCCGC AGTGAGAAGG TGATGCCCCA GCCGCTGGTG 
CTGTACTTCA GCATGTGTAT CCTGCAGATG GTGGAGCAGC TGCATCAAGC CAAAATCATC 
CACGCCGACA TCAAACCTGA CAACTTCCTG CTTGGAGAAA GGTTTCTGGA GAACGAGGAT 
TTGGATGCTG AGAGTCTGGA TCACGGCTTG GTTCTGATAG ATTTTGGTCA AAGTATTGAT 
ATGACCCTGT TCCCTGAGGG CACGGCCTTC ACTGCCCGCT GCATGACTTC TGGCTTCCAG 
TGCACAGAGA TGCTGAGTGG CCGGCCGTGG AACTACCAGA CTGATTACTT TGGCATTGCC 
GGCACCGTGC ACTGCATGAT CTTCGGGTCA TACATGCAAG TGAGAAATGA GGGTGGAGTT 
TGGAAAACGA ATGGCATTTT TAAAAGGAAT CCTCACAGTG ATCTATGGCA GGAGTTTTTC 
CATGTGCTGC TGAATGTTCC GGACTGTAAC TCTGCCTTAT GTTTGCGGAG TTTGCGTGAG 
CGTCTGTCTG TGGTGCTGCA GGAAAACTAC AGCAACAAAC TGCGCGGCCT TAAAAACCGA 
CTGGTGGTGC AGATCCTGGA GAGCCGCGGG GCTCGCAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007259948.1
CDS	152..4993
Translation	MDINSYLQQFEATVSSYVGEDPLDPWDKFVEFLECRLAAEERSNLCVVLERLVQTFLQDKRYRNDPRYINHCIKCAGYYGEPIKLYSYVHSLGVGARAAGLYVAWAQQFESKGLFTQAEQVYQRAIENQAEPMDTIQEQYRLFQSRMGKSQAASDHVRNPLQNSQLMNQNPRHRETLQPHCKEPDNSPLPADRTIRIISRSENNPMPRSSSSVESVPMYCVKELECEGSELSFEELRARRYFSKRQQLELLRKQEEVIWKHEEEEEEVIRMKKLLEELNSKLSTEQQETKHTFLQQELNESCSPLDRSVRPPLTPAMAEAAAPTSVTKEQAACSDIHRLQHAPECPLLNGRKSESHLLWQSTRVPASRLSLSLSAASQLAHSSALSAAVNRPLDLDTQAPVPLQLQDQRTASVCESQAERSLLESCSQRLAVFERDRSEKTEMDGGDANADNVIMDMFQAPTLLQDSVFNTTSQVADDSFEKNCRVNGFAPSVANPPSSMSFRIYQDENVKIGSEEQQVEKSKPAARALMEIPVSKPNVTPVGPESLTDESAMWGPQYASITACPNHTRDFAMAAHLVSTPLHPTAPYTWDTELNQVENTARGFSGTEENLYQRQPAKLSPILEQSPSDEKPSEVPECSLRAQGTIVGEGVSLPQQNQTLTHDQTSCSIGRQTLVPLALHDHTAEASRALNSSTKPSWSVYQSPERTSQTGSNTEPVSNSMLSVKQQIPHQLNSSNLQLNSSSSIKPSWSIYQSPEKTSHTGSNRGLNSSSMLSVREPQPIQSNANGLQMNSLSCSLAEASNALNSSAKPNWSIYQSPEKTSQTDCNTEPTSNSILSVKQYQADQFNLPSMQANSSSSTKPSWTIYQSPEKSHQASFCVEPKQHSNSMHLEAGEPKQDQFNSTGLQVNPDRTMNSTTQSPERTQKTQFSGDLHSTSMHLVAEPKQDQLNSTAIQLNSSCSTKPSWSIYQSPERTSQMGHSREFKADQQVNSNQGFNSSSLHLMGELKPDQLNSSINMCSGQGEADILLPKKSFHRRKSEKQLNFESLQVSRPVSHQSCTLPESPKPQPRIIQDVPMSPDPIPGSDWLQMDSPVPLSEPDLDVMVRSIRSQTIHRETPGAPASRSKLSWSVNQTPGRPSELQYVPQSPEPMEESDPHQLSKLEESGLLLSKRSFNRRKSDKRVLEKSLLSSQRQSSNQNQDVPMSPEPISGLNWLCPESPARVTESDLDVMMTPQQKSKSVDASAQLLQCDVPMSPLQASIPDYTPSVLVSDPWDEDLIDSLLSSLKTPLTSYPNLSLCTRGLPTLTPKMSVQMGEDSLRVDFVLGQGAFATVYQATNLTTSQKFILKVQKPANPWEFYINSQLNARVLRKERHLFNQLHSAHLFTNGSVLVGELHNCGTLLSVVNLYKSRSEKVMPQPLVLYFSMCILQMVEQLHQAKIIHADIKPDNFLLGERFLENEDLDAESLDHGLVLIDFGQSIDMTLFPEGTAFTARCMTSGFQCTEMLSGRPWNYQTDYFGIAGTVHCMIFGSYMQVRNEGGVWKTNGIFKRNPHSDLWQEFFHVLLNVPDCNSALCLRSLRERLSVVLQENYSNKLRGLKNRLVVQILESRGARR

Target ORF information:

RefSeq Version	XM_007259886.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007259886.2

ORF Insert Sequence:

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGA GCTGAATGAA 
TCGTGCAGCC CATTGGACCG GTCAGTAAGA CCACCTCTGA CCCCAGCCAT GGCTGAAGCT 
GCAGCTCCAA CATCAGTGAC AAAGGAACAG GCTGCGTGTT CAGACATACA TAGATTACAG 
CATGCACCAG AGTGCCCTCT GCTTAACGGG AGGAAGTCTG AGTCCCACTT GCTGTGGCAG 
AGCACAAGGG TTCCAGCTTC CAGACTGAGC CTGTCTCTGA GCGCAGCTTC ACAACTTGCA 
CACAGCAGTG CACTCAGTGC AGCTGTGAAT AGGCCATTGG ACCTGGACAC ACAGGCTCCA 
GTTCCACTAC AGCTGCAAGA CCAGAGAACC GCAAGCGTGT GTGAGAGCCA GGCTGAGCGT 
TCGCTTCTAG AAAGTTGCTC TCAGAGGCTG GCAGTGTTTG AAAGAGACAG ATCTGAAAAA 
ACAGAGATGG ATGGAGGAGA TGCTAATGCA GACAACGTGA TCATGGACAT GTTCCAGGCT 
CCAACGCTGC TGCAGGACTC CGTCTTCAAT ACTACCAGCC AGGTGGCTGA TGACAGCTTT 
GAGAAAAACT GCAGAGTAAA CGGTTTTGCC CCATCTGTAG CGAATCCACC TTCCTCAATG 
TCGTTCAGGA TATACCAGGA TGAAAATGTG AAGATTGGAA GTGAGGAACA ACAGGTGGAG 
AAGAGCAAAC CAGCAGCTCG AGCCCTGATG GAGATTCCGG TCTCCAAACC CAATGTCACT 
CCTGTAGGTC CGGAGTCTCT GACAGACGAG AGTGCGATGT GGGGTCCTCA ATATGCCTCC 
ATAACTGCCT GTCCAAATCA TACTCGTGAT TTTGCCATGG CTGCCCACCT GGTATCCACC 
CCCCTCCACC CCACTGCCCC GTACACCTGG GACACAGAGC TCAATCAGGT AGAAAACACT 
GCCAGAGGAT TTAGTGGGAC AGAGGAAAAC CTGTATCAGC GCCAGCCAGC CAAACTGAGT 
CCAATCCTGG AGCAGAGTCC TTCTGATGAG AAGCCGTCTG AAGTTCCTGA GTGCAGTCTG 
AGGGCTCAGG GCACCATCGT AGGAGAGGGA GTGAGTCTTC CTCAGCAGAA CCAGACGCTG 
ACCCATGACC AGACCAGCTG CAGCATCGGC AGGCAGACGC TGGTCCCCCT GGCTTTACAT 
GATCACACTG CCGAGGCCAG CAGAGCCCTC AACTCCAGCA CTAAACCTAG CTGGAGTGTC 
TACCAGAGCC CAGAGAGAAC CAGCCAGACA GGTTCTAACA CAGAACCTGT GTCCAACAGC 
ATGCTGTCTG TGAAACAGCA AATACCTCAC CAGCTCAATT CATCTAATCT ACAGCTTAAT 
TCCAGCTCTA GCATCAAACC CAGCTGGAGC ATCTACCAGA GCCCAGAGAA AACCAGCCAT 
ACGGGCTCGA ACAGAGGGCT CAACTCAAGC AGCATGCTGT CTGTAAGAGA GCCTCAACCG 
ATCCAATCCA ATGCAAATGG CCTACAGATG AACTCTCTAT CTTGCTCCCT TGCTGAGGCC 
AGCAATGCCC TCAACTCCAG TGCCAAACCT AACTGGAGCA TCTACCAGAG CCCAGAGAAA 
ACCAGCCAGA CAGATTGTAA CACAGAACCG ACCTCTAACA GCATACTGTC GGTAAAGCAG 
TACCAAGCAG ACCAGTTCAA TTTACCTAGC ATGCAGGCTA ACTCCAGCTC CAGCACTAAA 
CCCAGCTGGA CCATCTACCA AAGCCCAGAG AAATCCCACC AGGCTAGTTT TTGTGTGGAA 
CCCAAACAAC ACTCAAACAG CATGCACTTG GAAGCGGGAG AACCCAAACA GGACCAGTTC 
AACTCAACTG GCTTGCAGGT TAATCCAGAC AGAACCATGA ACTCCACCAC CCAAAGCCCA 
GAGAGAACCC AAAAGACTCA ATTTAGTGGG GACCTCCACT CAACCAGCAT GCATTTAGTG 
GCAGAACCCA AACAAGACCA GCTTAACTCA ACAGCCATTC AGCTGAACTC CAGCTGCAGC 
ACCAAACCCA GCTGGAGTAT TTACCAGAGC CCAGAGAGAA CCAGTCAAAT GGGTCACAGC 
AGGGAGTTCA AGGCAGACCA GCAGGTTAAC TCAAACCAAG GGTTCAACTC TTCTAGCCTG 
CATTTAATGG GAGAACTAAA ACCAGACCAA CTCAATTCCT CCATCAATAT GTGTTCTGGT 
CAGGGTGAGG CTGATATTCT CTTGCCCAAG AAGTCTTTTC ACCGGCGAAA ATCCGAAAAA 
CAGCTGAACT TCGAGAGCCT TCAGGTGAGC CGACCTGTAT CACACCAGTC CTGCACGCTG 
CCAGAGAGCC CAAAACCACA GCCCAGAATA ATCCAGGATG TTCCCATGAG TCCAGACCCA 
ATCCCAGGGT CCGACTGGCT TCAGATGGAC AGTCCAGTCC CGCTCTCTGA ACCCGACCTG 
GATGTGATGG TCAGGTCGAT CAGATCCCAG ACAATTCACA GAGAAACCCC CGGAGCTCCT 
GCTTCCAGGT CCAAACTCAG CTGGAGCGTG AACCAGACCC CAGGGAGGCC ATCTGAACTA 
CAGTACGTCC CGCAGAGTCC TGAGCCCATG GAGGAATCAG ATCCGCATCA GTTATCAAAG 
CTGGAAGAGT CTGGACTTCT GCTCTCCAAG AGATCCTTTA ACAGGCGCAA ATCGGATAAG 
AGGGTATTGG AGAAATCCCT CTTGTCGAGT CAGAGGCAGT CCTCCAACCA AAACCAGGAT 
GTTCCAATGA GTCCGGAACC AATTTCGGGG CTGAACTGGC TCTGTCCAGA GAGTCCAGCC 
CGTGTGACTG AGTCTGATCT GGACGTCATG ATGACACCTC AGCAAAAATC TAAATCAGTG 
GATGCTTCTG CCCAGCTGCT GCAGTGTGAT GTTCCAATGA GCCCCCTGCA GGCTTCCATA 
CCTGATTACA CTCCCTCAGT GTTGGTGTCT GACCCGTGGG ATGAGGACCT GATAGATTCT 
TTACTCTCCA GCCTGAAGAC GCCCCTCACC TCATACCCGA ACCTCAGCCT CTGCACTCGA 
GGCCTGCCCA CACTCACCCC CAAGATGTCC GTACAGATGG GTGAGGACTC TCTGCGGGTG 
GACTTTGTGT TGGGACAGGG TGCTTTCGCC ACCGTTTATC AGGCTACCAA CCTTACAACC 
TCACAAAAAT TCATCCTAAA GGTGCAGAAG CCGGCGAACC CATGGGAGTT TTACATCAAC 
AGCCAGCTAA ATGCTCGAGT GCTGCGGAAA GAGCGTCACC TGTTCAACCA GCTCCACTCC 
GCTCACCTGT TCACCAATGG CAGTGTGCTT GTGGGGGAAC TGCACAACTG CGGGACTCTG 
CTGAGTGTGG TGAATCTGTA TAAGAGCCGC AGTGAGAAGG TGATGCCCCA GCCGCTGGTG 
CTGTACTTCA GCATGTGTAT CCTGCAGATG GTGGAGCAGC TGCATCAAGC CAAAATCATC 
CACGCCGACA TCAAACCTGA CAACTTCCTG CTTGGAGAAA GGTTTCTGGA GAACGAGGAT 
TTGGATGCTG AGAGTCTGGA TCACGGCTTG GTTCTGATAG ATTTTGGTCA AAGTATTGAT 
ATGACCCTGT TCCCTGAGGG CACGGCCTTC ACTGCCCGCT GCATGACTTC TGGCTTCCAG 
TGCACAGAGA TGCTGAGTGG CCGGCCGTGG AACTACCAGA CTGATTACTT TGGCATTGCC 
GGCACCGTGC ACTGCATGAT CTTCGGGTCA TACATGCAAG TGAGAAATGA GGGTGGAGTT 
TGGAAAACGA ATGGCATTTT TAAAAGGAAT CCTCACAGTG ATCTATGGCA GGAGTTTTTC 
CATGTGCTGC TGAATGTTCC GGACTGTAAC TCTGCCTTAT GTTTGCGGAG TTTGCGTGAG 
CGTCTGTCTG TGGTGCTGCA GGAAAACTAC AGCAACAAAC TGCGCGGCCT TAAAAACCGA 
CTGGTGGTGC AGATCCTGGA GAGCCGCGGG GCTCGCAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_015608508.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015463994.1 Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.

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CloneID	OAs41542
Clone ID Related Accession (Same CDS sequence)	XM_015608508.1
Accession Version	XM_015608508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749470.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGT TCCAGCTTCC 
AGACTGAGCC TGTCTCTGAG CGCAGCTTCA CAACTTGCAC ACAGCAGTGC ACTCAGTGCA 
GCTGTGAATA GGCCATTGGA CCTGGACACA CAGGCTCCAG TTCCACTACA GCTGCAAGAC 
CAGAGAACCG CAAGCGTGTG TGAGAGCCAG GCTGAGCGTT CGCTTCTAGA AAGTTGCTCT 
CAGAGGCTGG CAGTGTTTGA AAGAGACAGA TCTGAAAAAA CAGAGATGGA TGGAGGAGAT 
GCTAATGCAG ACACATCTCA CGGAGGCTTT AATCATTCTC ATGTCACTCC TAACACATCT 
CTGGGTCTGA TTTCGGCGAC TCCTTCACGT GCACTCCCCT CTCCCACCGT CAACACTCGG 
GAGGCTCTGG ACGTGATCAT GGACATGTTC CAGGCTCCAA CGCTGCTGCA GGACTCCGTC 
TTCAATACTA CCAGCCAGGT GGCTGATGAC AGCTTTGAGA AAAACTGCAG AGTAAACGGT 
TTTGCCCCAT CTGTAGCGAA TCCACCTTCC TCAATGTCGT TCAGGATATA CCAGGATGAA 
AATGTGAAGA TTGGAAGTGA GGAACAACAG GTGGAGAAGA GCAAACCAGC AGCTCGAGCC 
CTGATGGAGA TTCCGGTCTC CAAACCCAAT GTCACTCCTG TAGGTCCGGA GTCTCTGACA 
GACGAGAGTG CGATGTGGGG TCCTCAATAT GCCTCCATAA CTGCCTGTCC AAATCATACT 
CGTGATTTTG CCATGGCTGC CCACCTGGTA TCCACCCCCC TCCACCCCAC TGCCCCGTAC 
ACCTGGGACA CAGAGCTCAA TCAGGTAGAA AACACTGCCA GAGGATTTAG TGGGACAGAG 
GAAAACCTGT ATCAGCGCCA GCCAGCCAAA CTGAGTCCAA TCCTGGAGCA GAGTCCTTCT 
GATGAGAAGC CGTCTGAAGT TCCTGAGTGC AGTCTGAGGG CTCAGGGCAC CATCGTAGGA 
GAGGGAGTGA GTCTTCCTCA GCAGAACCAG ACGCTGACCC ATGACCAGAC CAGCTGCAGC 
ATCGGCAGGC AGACGCTGGT CCCCCTGGCT TTACATGATC ACACTGCCGA GGCCAGCAGA 
GCCCTCAACT CCAGCACTAA ACCTAGCTGG AGTGTCTACC AGAGCCCAGA GAGAACCAGC 
CAGACAGGTT CTAACACAGA ACCTGTGTCC AACAGCATGC TGTCTGTGAA ACAGCAAATA 
CCTCACCAGC TCAATTCATC TAATCTACAG CTTAATTCCA GCTCTAGCAT CAAACCCAGC 
TGGAGCATCT ACCAGAGCCC AGAGAAAACC AGCCATACGG GCTCGAACAG AGGGCTCAAC 
TCAAGCAGCA TGCTGTCTGT AAGAGAGCCT CAACCGATCC AATCCAATGC AAATGGCCTA 
CAGATGAACT CTCTATCTTG CTCCCTTGCT GAGGCCAGCA ATGCCCTCAA CTCCAGTGCC 
AAACCTAACT GGAGCATCTA CCAGAGCCCA GAGAAAACCA GCCAGACAGA TTGTAACACA 
GAACCGACCT CTAACAGCAT ACTGTCGGTA AAGCAGTACC AAGCAGACCA GTTCAATTTA 
CCTAGCATGC AGGCTAACTC CAGCTCCAGC ACTAAACCCA GCTGGACCAT CTACCAAAGC 
CCAGAGAAAT CCCACCAGGC TAGTTTTTGT GTGGAACCCA AACAACACTC AAACAGCATG 
CACTTGGAAG CGGGAGAACC CAAACAGGAC CAGTTCAACT CAACTGGCTT GCAGGTTAAT 
CCAGACAGAA CCATGAACTC CACCACCCAA AGCCCAGAGA GAACCCAAAA GACTCAATTT 
AGTGGGGACC TCCACTCAAC CAGCATGCAT TTAGTGGCAG AACCCAAACA AGACCAGCTT 
AACTCAACAG CCATTCAGCT GAACTCCAGC TGCAGCACCA AACCCAGCTG GAGTATTTAC 
CAGAGCCCAG AGAGAACCAG TCAAATGGGT CACAGCAGGG AGTTCAAGGC AGACCAGCAG 
GTTAACTCAA ACCAAGGGTT CAACTCTTCT AGCCTGCATT TAATGGGAGA ACTAAAACCA 
GACCAACTCA ATTCCTCCAT CAATATGTGT TCTGGTCAGG GTGAGGCTGA TATTCTCTTG 
CCCAAGAAGT CTTTTCACCG GCGAAAATCC GAAAAACAGC TGAACTTCGA GAGCCTTCAG 
GTGAGCCGAC CTGTATCACA CCAGTCCTGC ACGCTGCCAG AGAGCCCAAA ACCACAGCCC 
AGAATAATCC AGGATGTTCC CATGAGTCCA GACCCAATCC CAGGGTCCGA CTGGCTTCAG 
ATGGACAGTC CAGTCCCGCT CTCTGAACCC GACCTGGATG TGATGGTCAG GTCGATCAGA 
TCCCAGACAA TTCACAGAGA AACCCCCGGA GCTCCTGCTT CCAGGTCCAA ACTCAGCTGG 
AGCGTGAACC AGACCCCAGG GAGGCCATCT GAACTACAGT ACGTCCCGCA GAGTCCTGAG 
CCCATGGAGG AATCAGATCC GCATCAGTTA TCAAAGCTGG AAGAGTCTGG ACTTCTGCTC 
TCCAAGAGAT CCTTTAACAG GCGCAAATCG GATAAGAGGG TATTGGAGAA ATCCCTCTTG 
TCGAGTCAGA GGCAGTCCTC CAACCAAAAC CAGGATGTTC CAATGAGTCC GGAACCAATT 
TCGGGGCTGA ACTGGCTCTG TCCAGAGAGT CCAGCCCGTG TGACTGAGTC TGATCTGGAC 
GTCATGATGA CACCTCAGCA AAAATCTAAA TCAGTGGATG CTTCTGCCCA GCTGCTGCAG 
TGTGATGTTC CAATGAGCCC CCTGCAGGCT TCCATACCTG ATTACACTCC CTCAGTGTTG 
GTGTCTGACC CGTGGGATGA GGACCTGATA GATTCTTTAC TCTCCAGCCT GAAGACGCCC 
CTCACCTCAT ACCCGAACCT CAGCCTCTGC ACTCGAGGCC TGCCCACACT CACCCCCAAG 
ATGTCCGTAC AGATGGGTGA GGACTCTCTG CGGGTGGACT TTGTGTTGGG ACAGGGTGCT 
TTCGCCACCG TTTATCAGGC TACCAACCTT ACAACCTCAC AAAAATTCAT CCTAAAGGTG 
CAGAAGCCGG CGAACCCATG GGAGTTTTAC ATCAACAGCC AGCTAAATGC TCGAGTGCTG 
CGGAAAGAGC GTCACCTGTT CAACCAGCTC CACTCCGCTC ACCTGTTCAC CAATGGCAGT 
GTGCTTGTGG GGGAACTGCA CAACTGCGGG ACTCTGCTGA GTGTGGTGAA TCTGTATAAG 
AGCCGCAGTG AGAAGGTGAT GCCCCAGCCG CTGGTGCTGT ACTTCAGCAT GTGTATCCTG 
CAGATGGTGG AGCAGCTGCA TCAAGCCAAA ATCATCCACG CCGACATCAA ACCTGACAAC 
TTCCTGCTTG GAGAAAGGTT TCTGGAGAAC GAGGATTTGG ATGCTGAGAG TCTGGATCAC 
GGCTTGGTTC TGATAGATTT TGGTCAAAGT ATTGATATGA CCCTGTTCCC TGAGGGCACG 
GCCTTCACTG CCCGCTGCAT GACTTCTGGC TTCCAGTGCA CAGAGATGCT GAGTGGCCGG 
CCGTGGAACT ACCAGACTGA TTACTTTGGC ATTGCCGGCA CCGTGCACTG CATGATCTTC 
GGGTCATACA TGCAAGTGAG AAATGAGGGT GGAGTTTGGA AAACGAATGG CATTTTTAAA 
AGGAATCCTC ACAGTGATCT ATGGCAGGAG TTTTTCCATG TGCTGCTGAA TGTTCCGGAC 
TGTAACTCTG CCTTATGTTT GCGGAGTTTG CGTGAGCGTC TGTCTGTGGT GCTGCAGGAA 
AACTACAGCA ACAAACTGCG CGGCCTTAAA AACCGACTGG TGGTGCAGAT CCTGGAGAGC 
CGCGGGGCTC GCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015463994.1
CDS	152..4909
Translation	MDINSYLQQFEATVSSYVGEDPLDPWDKFVEFLECRLAAEERSNLCVVLERLVQTFLQDKRYRNDPRYINHCIKCAGYYGEPIKLYSYVHSLGVGARAAGLYVAWAQQFESKGLFTQAEQVYQRAIENQAEPMDTIQEQYRLFQSRMGKSQAASDHVRNPLQNSQLMNQNPRHRETLQPHCKEPDNSPLPADRTIRIISRSENNPMPRSSSSVESVPMYCVKELECEGSELSFEELRARRYFSKRQQLELLRKQEEVIWKHEEEEEEVIRMKKLLEELNSKLSTEQQETKHTFLQQVPASRLSLSLSAASQLAHSSALSAAVNRPLDLDTQAPVPLQLQDQRTASVCESQAERSLLESCSQRLAVFERDRSEKTEMDGGDANADTSHGGFNHSHVTPNTSLGLISATPSRALPSPTVNTREALDVIMDMFQAPTLLQDSVFNTTSQVADDSFEKNCRVNGFAPSVANPPSSMSFRIYQDENVKIGSEEQQVEKSKPAARALMEIPVSKPNVTPVGPESLTDESAMWGPQYASITACPNHTRDFAMAAHLVSTPLHPTAPYTWDTELNQVENTARGFSGTEENLYQRQPAKLSPILEQSPSDEKPSEVPECSLRAQGTIVGEGVSLPQQNQTLTHDQTSCSIGRQTLVPLALHDHTAEASRALNSSTKPSWSVYQSPERTSQTGSNTEPVSNSMLSVKQQIPHQLNSSNLQLNSSSSIKPSWSIYQSPEKTSHTGSNRGLNSSSMLSVREPQPIQSNANGLQMNSLSCSLAEASNALNSSAKPNWSIYQSPEKTSQTDCNTEPTSNSILSVKQYQADQFNLPSMQANSSSSTKPSWTIYQSPEKSHQASFCVEPKQHSNSMHLEAGEPKQDQFNSTGLQVNPDRTMNSTTQSPERTQKTQFSGDLHSTSMHLVAEPKQDQLNSTAIQLNSSCSTKPSWSIYQSPERTSQMGHSREFKADQQVNSNQGFNSSSLHLMGELKPDQLNSSINMCSGQGEADILLPKKSFHRRKSEKQLNFESLQVSRPVSHQSCTLPESPKPQPRIIQDVPMSPDPIPGSDWLQMDSPVPLSEPDLDVMVRSIRSQTIHRETPGAPASRSKLSWSVNQTPGRPSELQYVPQSPEPMEESDPHQLSKLEESGLLLSKRSFNRRKSDKRVLEKSLLSSQRQSSNQNQDVPMSPEPISGLNWLCPESPARVTESDLDVMMTPQQKSKSVDASAQLLQCDVPMSPLQASIPDYTPSVLVSDPWDEDLIDSLLSSLKTPLTSYPNLSLCTRGLPTLTPKMSVQMGEDSLRVDFVLGQGAFATVYQATNLTTSQKFILKVQKPANPWEFYINSQLNARVLRKERHLFNQLHSAHLFTNGSVLVGELHNCGTLLSVVNLYKSRSEKVMPQPLVLYFSMCILQMVEQLHQAKIIHADIKPDNFLLGERFLENEDLDAESLDHGLVLIDFGQSIDMTLFPEGTAFTARCMTSGFQCTEMLSGRPWNYQTDYFGIAGTVHCMIFGSYMQVRNEGGVWKTNGIFKRNPHSDLWQEFFHVLLNVPDCNSALCLRSLRERLSVVLQENYSNKLRGLKNRLVVQILESRGARR

Target ORF information:

RefSeq Version	XM_015608508.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015608508.1

ORF Insert Sequence:

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGT TCCAGCTTCC 
AGACTGAGCC TGTCTCTGAG CGCAGCTTCA CAACTTGCAC ACAGCAGTGC ACTCAGTGCA 
GCTGTGAATA GGCCATTGGA CCTGGACACA CAGGCTCCAG TTCCACTACA GCTGCAAGAC 
CAGAGAACCG CAAGCGTGTG TGAGAGCCAG GCTGAGCGTT CGCTTCTAGA AAGTTGCTCT 
CAGAGGCTGG CAGTGTTTGA AAGAGACAGA TCTGAAAAAA CAGAGATGGA TGGAGGAGAT 
GCTAATGCAG ACACATCTCA CGGAGGCTTT AATCATTCTC ATGTCACTCC TAACACATCT 
CTGGGTCTGA TTTCGGCGAC TCCTTCACGT GCACTCCCCT CTCCCACCGT CAACACTCGG 
GAGGCTCTGG ACGTGATCAT GGACATGTTC CAGGCTCCAA CGCTGCTGCA GGACTCCGTC 
TTCAATACTA CCAGCCAGGT GGCTGATGAC AGCTTTGAGA AAAACTGCAG AGTAAACGGT 
TTTGCCCCAT CTGTAGCGAA TCCACCTTCC TCAATGTCGT TCAGGATATA CCAGGATGAA 
AATGTGAAGA TTGGAAGTGA GGAACAACAG GTGGAGAAGA GCAAACCAGC AGCTCGAGCC 
CTGATGGAGA TTCCGGTCTC CAAACCCAAT GTCACTCCTG TAGGTCCGGA GTCTCTGACA 
GACGAGAGTG CGATGTGGGG TCCTCAATAT GCCTCCATAA CTGCCTGTCC AAATCATACT 
CGTGATTTTG CCATGGCTGC CCACCTGGTA TCCACCCCCC TCCACCCCAC TGCCCCGTAC 
ACCTGGGACA CAGAGCTCAA TCAGGTAGAA AACACTGCCA GAGGATTTAG TGGGACAGAG 
GAAAACCTGT ATCAGCGCCA GCCAGCCAAA CTGAGTCCAA TCCTGGAGCA GAGTCCTTCT 
GATGAGAAGC CGTCTGAAGT TCCTGAGTGC AGTCTGAGGG CTCAGGGCAC CATCGTAGGA 
GAGGGAGTGA GTCTTCCTCA GCAGAACCAG ACGCTGACCC ATGACCAGAC CAGCTGCAGC 
ATCGGCAGGC AGACGCTGGT CCCCCTGGCT TTACATGATC ACACTGCCGA GGCCAGCAGA 
GCCCTCAACT CCAGCACTAA ACCTAGCTGG AGTGTCTACC AGAGCCCAGA GAGAACCAGC 
CAGACAGGTT CTAACACAGA ACCTGTGTCC AACAGCATGC TGTCTGTGAA ACAGCAAATA 
CCTCACCAGC TCAATTCATC TAATCTACAG CTTAATTCCA GCTCTAGCAT CAAACCCAGC 
TGGAGCATCT ACCAGAGCCC AGAGAAAACC AGCCATACGG GCTCGAACAG AGGGCTCAAC 
TCAAGCAGCA TGCTGTCTGT AAGAGAGCCT CAACCGATCC AATCCAATGC AAATGGCCTA 
CAGATGAACT CTCTATCTTG CTCCCTTGCT GAGGCCAGCA ATGCCCTCAA CTCCAGTGCC 
AAACCTAACT GGAGCATCTA CCAGAGCCCA GAGAAAACCA GCCAGACAGA TTGTAACACA 
GAACCGACCT CTAACAGCAT ACTGTCGGTA AAGCAGTACC AAGCAGACCA GTTCAATTTA 
CCTAGCATGC AGGCTAACTC CAGCTCCAGC ACTAAACCCA GCTGGACCAT CTACCAAAGC 
CCAGAGAAAT CCCACCAGGC TAGTTTTTGT GTGGAACCCA AACAACACTC AAACAGCATG 
CACTTGGAAG CGGGAGAACC CAAACAGGAC CAGTTCAACT CAACTGGCTT GCAGGTTAAT 
CCAGACAGAA CCATGAACTC CACCACCCAA AGCCCAGAGA GAACCCAAAA GACTCAATTT 
AGTGGGGACC TCCACTCAAC CAGCATGCAT TTAGTGGCAG AACCCAAACA AGACCAGCTT 
AACTCAACAG CCATTCAGCT GAACTCCAGC TGCAGCACCA AACCCAGCTG GAGTATTTAC 
CAGAGCCCAG AGAGAACCAG TCAAATGGGT CACAGCAGGG AGTTCAAGGC AGACCAGCAG 
GTTAACTCAA ACCAAGGGTT CAACTCTTCT AGCCTGCATT TAATGGGAGA ACTAAAACCA 
GACCAACTCA ATTCCTCCAT CAATATGTGT TCTGGTCAGG GTGAGGCTGA TATTCTCTTG 
CCCAAGAAGT CTTTTCACCG GCGAAAATCC GAAAAACAGC TGAACTTCGA GAGCCTTCAG 
GTGAGCCGAC CTGTATCACA CCAGTCCTGC ACGCTGCCAG AGAGCCCAAA ACCACAGCCC 
AGAATAATCC AGGATGTTCC CATGAGTCCA GACCCAATCC CAGGGTCCGA CTGGCTTCAG 
ATGGACAGTC CAGTCCCGCT CTCTGAACCC GACCTGGATG TGATGGTCAG GTCGATCAGA 
TCCCAGACAA TTCACAGAGA AACCCCCGGA GCTCCTGCTT CCAGGTCCAA ACTCAGCTGG 
AGCGTGAACC AGACCCCAGG GAGGCCATCT GAACTACAGT ACGTCCCGCA GAGTCCTGAG 
CCCATGGAGG AATCAGATCC GCATCAGTTA TCAAAGCTGG AAGAGTCTGG ACTTCTGCTC 
TCCAAGAGAT CCTTTAACAG GCGCAAATCG GATAAGAGGG TATTGGAGAA ATCCCTCTTG 
TCGAGTCAGA GGCAGTCCTC CAACCAAAAC CAGGATGTTC CAATGAGTCC GGAACCAATT 
TCGGGGCTGA ACTGGCTCTG TCCAGAGAGT CCAGCCCGTG TGACTGAGTC TGATCTGGAC 
GTCATGATGA CACCTCAGCA AAAATCTAAA TCAGTGGATG CTTCTGCCCA GCTGCTGCAG 
TGTGATGTTC CAATGAGCCC CCTGCAGGCT TCCATACCTG ATTACACTCC CTCAGTGTTG 
GTGTCTGACC CGTGGGATGA GGACCTGATA GATTCTTTAC TCTCCAGCCT GAAGACGCCC 
CTCACCTCAT ACCCGAACCT CAGCCTCTGC ACTCGAGGCC TGCCCACACT CACCCCCAAG 
ATGTCCGTAC AGATGGGTGA GGACTCTCTG CGGGTGGACT TTGTGTTGGG ACAGGGTGCT 
TTCGCCACCG TTTATCAGGC TACCAACCTT ACAACCTCAC AAAAATTCAT CCTAAAGGTG 
CAGAAGCCGG CGAACCCATG GGAGTTTTAC ATCAACAGCC AGCTAAATGC TCGAGTGCTG 
CGGAAAGAGC GTCACCTGTT CAACCAGCTC CACTCCGCTC ACCTGTTCAC CAATGGCAGT 
GTGCTTGTGG GGGAACTGCA CAACTGCGGG ACTCTGCTGA GTGTGGTGAA TCTGTATAAG 
AGCCGCAGTG AGAAGGTGAT GCCCCAGCCG CTGGTGCTGT ACTTCAGCAT GTGTATCCTG 
CAGATGGTGG AGCAGCTGCA TCAAGCCAAA ATCATCCACG CCGACATCAA ACCTGACAAC 
TTCCTGCTTG GAGAAAGGTT TCTGGAGAAC GAGGATTTGG ATGCTGAGAG TCTGGATCAC 
GGCTTGGTTC TGATAGATTT TGGTCAAAGT ATTGATATGA CCCTGTTCCC TGAGGGCACG 
GCCTTCACTG CCCGCTGCAT GACTTCTGGC TTCCAGTGCA CAGAGATGCT GAGTGGCCGG 
CCGTGGAACT ACCAGACTGA TTACTTTGGC ATTGCCGGCA CCGTGCACTG CATGATCTTC 
GGGTCATACA TGCAAGTGAG AAATGAGGGT GGAGTTTGGA AAACGAATGG CATTTTTAAA 
AGGAATCCTC ACAGTGATCT ATGGCAGGAG TTTTTCCATG TGCTGCTGAA TGTTCCGGAC 
TGTAACTCTG CCTTATGTTT GCGGAGTTTG CGTGAGCGTC TGTCTGTGGT GCTGCAGGAA 
AACTACAGCA ACAAACTGCG CGGCCTTAAA AACCGACTGG TGGTGCAGAT CCTGGAGAGC 
CGCGGGGCTC GCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bub1 ( XM_007259887.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007259949.1 Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X5, mRNA.

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CloneID	OAs41543
Clone ID Related Accession (Same CDS sequence)	XM_007259887.2
Accession Version	XM_007259887.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749470.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007259887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGT TCCAGCTTCC 
AGACTGAGCC TGTCTCTGAG CGCAGCTTCA CAACTTGCAC ACAGCAGTGC ACTCAGTGCA 
GCTGTGAATA GGCCATTGGA CCTGGACACA CAGGCTCCAG TTCCACTACA GCTGCAAGAC 
CAGAGAACCG CAAGCGTGTG TGAGAGCCAG GCTGAGCGTT CGCTTCTAGA AAGTTGCTCT 
CAGAGGCTGG CAGTGTTTGA AAGAGACAGA TCTGAAAAAA CAGAGATGGA TGGAGGAGAT 
GCTAATGCAG ACAACGTGAT CATGGACATG TTCCAGGCTC CAACGCTGCT GCAGGACTCC 
GTCTTCAATA CTACCAGCCA GGTGGCTGAT GACAGCTTTG AGAAAAACTG CAGAGTAAAC 
GGTTTTGCCC CATCTGTAGC GAATCCACCT TCCTCAATGT CGTTCAGGAT ATACCAGGAT 
GAAAATGTGA AGATTGGAAG TGAGGAACAA CAGGTGGAGA AGAGCAAACC AGCAGCTCGA 
GCCCTGATGG AGATTCCGGT CTCCAAACCC AATGTCACTC CTGTAGGTCC GGAGTCTCTG 
ACAGACGAGA GTGCGATGTG GGGTCCTCAA TATGCCTCCA TAACTGCCTG TCCAAATCAT 
ACTCGTGATT TTGCCATGGC TGCCCACCTG GTATCCACCC CCCTCCACCC CACTGCCCCG 
TACACCTGGG ACACAGAGCT CAATCAGGTA GAAAACACTG CCAGAGGATT TAGTGGGACA 
GAGGAAAACC TGTATCAGCG CCAGCCAGCC AAACTGAGTC CAATCCTGGA GCAGAGTCCT 
TCTGATGAGA AGCCGTCTGA AGTTCCTGAG TGCAGTCTGA GGGCTCAGGG CACCATCGTA 
GGAGAGGGAG TGAGTCTTCC TCAGCAGAAC CAGACGCTGA CCCATGACCA GACCAGCTGC 
AGCATCGGCA GGCAGACGCT GGTCCCCCTG GCTTTACATG ATCACACTGC CGAGGCCAGC 
AGAGCCCTCA ACTCCAGCAC TAAACCTAGC TGGAGTGTCT ACCAGAGCCC AGAGAGAACC 
AGCCAGACAG GTTCTAACAC AGAACCTGTG TCCAACAGCA TGCTGTCTGT GAAACAGCAA 
ATACCTCACC AGCTCAATTC ATCTAATCTA CAGCTTAATT CCAGCTCTAG CATCAAACCC 
AGCTGGAGCA TCTACCAGAG CCCAGAGAAA ACCAGCCATA CGGGCTCGAA CAGAGGGCTC 
AACTCAAGCA GCATGCTGTC TGTAAGAGAG CCTCAACCGA TCCAATCCAA TGCAAATGGC 
CTACAGATGA ACTCTCTATC TTGCTCCCTT GCTGAGGCCA GCAATGCCCT CAACTCCAGT 
GCCAAACCTA ACTGGAGCAT CTACCAGAGC CCAGAGAAAA CCAGCCAGAC AGATTGTAAC 
ACAGAACCGA CCTCTAACAG CATACTGTCG GTAAAGCAGT ACCAAGCAGA CCAGTTCAAT 
TTACCTAGCA TGCAGGCTAA CTCCAGCTCC AGCACTAAAC CCAGCTGGAC CATCTACCAA 
AGCCCAGAGA AATCCCACCA GGCTAGTTTT TGTGTGGAAC CCAAACAACA CTCAAACAGC 
ATGCACTTGG AAGCGGGAGA ACCCAAACAG GACCAGTTCA ACTCAACTGG CTTGCAGGTT 
AATCCAGACA GAACCATGAA CTCCACCACC CAAAGCCCAG AGAGAACCCA AAAGACTCAA 
TTTAGTGGGG ACCTCCACTC AACCAGCATG CATTTAGTGG CAGAACCCAA ACAAGACCAG 
CTTAACTCAA CAGCCATTCA GCTGAACTCC AGCTGCAGCA CCAAACCCAG CTGGAGTATT 
TACCAGAGCC CAGAGAGAAC CAGTCAAATG GGTCACAGCA GGGAGTTCAA GGCAGACCAG 
CAGGTTAACT CAAACCAAGG GTTCAACTCT TCTAGCCTGC ATTTAATGGG AGAACTAAAA 
CCAGACCAAC TCAATTCCTC CATCAATATG TGTTCTGGTC AGGGTGAGGC TGATATTCTC 
TTGCCCAAGA AGTCTTTTCA CCGGCGAAAA TCCGAAAAAC AGCTGAACTT CGAGAGCCTT 
CAGGTGAGCC GACCTGTATC ACACCAGTCC TGCACGCTGC CAGAGAGCCC AAAACCACAG 
CCCAGAATAA TCCAGGATGT TCCCATGAGT CCAGACCCAA TCCCAGGGTC CGACTGGCTT 
CAGATGGACA GTCCAGTCCC GCTCTCTGAA CCCGACCTGG ATGTGATGGT CAGGTCGATC 
AGATCCCAGA CAATTCACAG AGAAACCCCC GGAGCTCCTG CTTCCAGGTC CAAACTCAGC 
TGGAGCGTGA ACCAGACCCC AGGGAGGCCA TCTGAACTAC AGTACGTCCC GCAGAGTCCT 
GAGCCCATGG AGGAATCAGA TCCGCATCAG TTATCAAAGC TGGAAGAGTC TGGACTTCTG 
CTCTCCAAGA GATCCTTTAA CAGGCGCAAA TCGGATAAGA GGGTATTGGA GAAATCCCTC 
TTGTCGAGTC AGAGGCAGTC CTCCAACCAA AACCAGGATG TTCCAATGAG TCCGGAACCA 
ATTTCGGGGC TGAACTGGCT CTGTCCAGAG AGTCCAGCCC GTGTGACTGA GTCTGATCTG 
GACGTCATGA TGACACCTCA GCAAAAATCT AAATCAGTGG ATGCTTCTGC CCAGCTGCTG 
CAGTGTGATG TTCCAATGAG CCCCCTGCAG GCTTCCATAC CTGATTACAC TCCCTCAGTG 
TTGGTGTCTG ACCCGTGGGA TGAGGACCTG ATAGATTCTT TACTCTCCAG CCTGAAGACG 
CCCCTCACCT CATACCCGAA CCTCAGCCTC TGCACTCGAG GCCTGCCCAC ACTCACCCCC 
AAGATGTCCG TACAGATGGG TGAGGACTCT CTGCGGGTGG ACTTTGTGTT GGGACAGGGT 
GCTTTCGCCA CCGTTTATCA GGCTACCAAC CTTACAACCT CACAAAAATT CATCCTAAAG 
GTGCAGAAGC CGGCGAACCC ATGGGAGTTT TACATCAACA GCCAGCTAAA TGCTCGAGTG 
CTGCGGAAAG AGCGTCACCT GTTCAACCAG CTCCACTCCG CTCACCTGTT CACCAATGGC 
AGTGTGCTTG TGGGGGAACT GCACAACTGC GGGACTCTGC TGAGTGTGGT GAATCTGTAT 
AAGAGCCGCA GTGAGAAGGT GATGCCCCAG CCGCTGGTGC TGTACTTCAG CATGTGTATC 
CTGCAGATGG TGGAGCAGCT GCATCAAGCC AAAATCATCC ACGCCGACAT CAAACCTGAC 
AACTTCCTGC TTGGAGAAAG GTTTCTGGAG AACGAGGATT TGGATGCTGA GAGTCTGGAT 
CACGGCTTGG TTCTGATAGA TTTTGGTCAA AGTATTGATA TGACCCTGTT CCCTGAGGGC 
ACGGCCTTCA CTGCCCGCTG CATGACTTCT GGCTTCCAGT GCACAGAGAT GCTGAGTGGC 
CGGCCGTGGA ACTACCAGAC TGATTACTTT GGCATTGCCG GCACCGTGCA CTGCATGATC 
TTCGGGTCAT ACATGCAAGT GAGAAATGAG GGTGGAGTTT GGAAAACGAA TGGCATTTTT 
AAAAGGAATC CTCACAGTGA TCTATGGCAG GAGTTTTTCC ATGTGCTGCT GAATGTTCCG 
GACTGTAACT CTGCCTTATG TTTGCGGAGT TTGCGTGAGC GTCTGTCTGT GGTGCTGCAG 
GAAAACTACA GCAACAAACT GCGCGGCCTT AAAAACCGAC TGGTGGTGCA GATCCTGGAG 
AGCCGCGGGG CTCGCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007259949.1
CDS	152..4792
Translation	MDINSYLQQFEATVSSYVGEDPLDPWDKFVEFLECRLAAEERSNLCVVLERLVQTFLQDKRYRNDPRYINHCIKCAGYYGEPIKLYSYVHSLGVGARAAGLYVAWAQQFESKGLFTQAEQVYQRAIENQAEPMDTIQEQYRLFQSRMGKSQAASDHVRNPLQNSQLMNQNPRHRETLQPHCKEPDNSPLPADRTIRIISRSENNPMPRSSSSVESVPMYCVKELECEGSELSFEELRARRYFSKRQQLELLRKQEEVIWKHEEEEEEVIRMKKLLEELNSKLSTEQQETKHTFLQQVPASRLSLSLSAASQLAHSSALSAAVNRPLDLDTQAPVPLQLQDQRTASVCESQAERSLLESCSQRLAVFERDRSEKTEMDGGDANADNVIMDMFQAPTLLQDSVFNTTSQVADDSFEKNCRVNGFAPSVANPPSSMSFRIYQDENVKIGSEEQQVEKSKPAARALMEIPVSKPNVTPVGPESLTDESAMWGPQYASITACPNHTRDFAMAAHLVSTPLHPTAPYTWDTELNQVENTARGFSGTEENLYQRQPAKLSPILEQSPSDEKPSEVPECSLRAQGTIVGEGVSLPQQNQTLTHDQTSCSIGRQTLVPLALHDHTAEASRALNSSTKPSWSVYQSPERTSQTGSNTEPVSNSMLSVKQQIPHQLNSSNLQLNSSSSIKPSWSIYQSPEKTSHTGSNRGLNSSSMLSVREPQPIQSNANGLQMNSLSCSLAEASNALNSSAKPNWSIYQSPEKTSQTDCNTEPTSNSILSVKQYQADQFNLPSMQANSSSSTKPSWTIYQSPEKSHQASFCVEPKQHSNSMHLEAGEPKQDQFNSTGLQVNPDRTMNSTTQSPERTQKTQFSGDLHSTSMHLVAEPKQDQLNSTAIQLNSSCSTKPSWSIYQSPERTSQMGHSREFKADQQVNSNQGFNSSSLHLMGELKPDQLNSSINMCSGQGEADILLPKKSFHRRKSEKQLNFESLQVSRPVSHQSCTLPESPKPQPRIIQDVPMSPDPIPGSDWLQMDSPVPLSEPDLDVMVRSIRSQTIHRETPGAPASRSKLSWSVNQTPGRPSELQYVPQSPEPMEESDPHQLSKLEESGLLLSKRSFNRRKSDKRVLEKSLLSSQRQSSNQNQDVPMSPEPISGLNWLCPESPARVTESDLDVMMTPQQKSKSVDASAQLLQCDVPMSPLQASIPDYTPSVLVSDPWDEDLIDSLLSSLKTPLTSYPNLSLCTRGLPTLTPKMSVQMGEDSLRVDFVLGQGAFATVYQATNLTTSQKFILKVQKPANPWEFYINSQLNARVLRKERHLFNQLHSAHLFTNGSVLVGELHNCGTLLSVVNLYKSRSEKVMPQPLVLYFSMCILQMVEQLHQAKIIHADIKPDNFLLGERFLENEDLDAESLDHGLVLIDFGQSIDMTLFPEGTAFTARCMTSGFQCTEMLSGRPWNYQTDYFGIAGTVHCMIFGSYMQVRNEGGVWKTNGIFKRNPHSDLWQEFFHVLLNVPDCNSALCLRSLRERLSVVLQENYSNKLRGLKNRLVVQILESRGARR

Target ORF information:

RefSeq Version	XM_007259887.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus BUB1 mitotic checkpoint serine/threonine kinase (bub1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007259887.2

ORF Insert Sequence:

ATGGATATTA ACAGTTATTT ACAGCAATTT GAAGCCACCG TGAGCAGTTA TGTTGGAGAA 
GATCCTCTTG ATCCATGGGA TAAGTTTGTG GAGTTTCTGG AGTGCAGGCT GGCAGCAGAG 
GAGAGGAGTA ATCTGTGTGT GGTGCTGGAG AGGTTGGTTC AGACGTTTCT GCAGGATAAA 
CGCTACCGCA ATGACCCCAG ATACATCAAC CACTGCATCA AATGTGCGGG GTATTACGGA 
GAGCCCATAA AGCTGTACAG TTATGTGCAC AGTCTGGGTG TGGGGGCTCG GGCAGCAGGG 
CTGTACGTGG CCTGGGCTCA GCAGTTCGAG AGTAAAGGTC TGTTCACTCA GGCTGAGCAG 
GTCTACCAGA GAGCCATCGA GAACCAGGCG GAACCCATGG ACACGATCCA GGAGCAGTAC 
AGGCTGTTCC AGAGTAGAAT GGGTAAAAGC CAAGCAGCCT CAGATCATGT GAGAAATCCT 
CTGCAAAATT CCCAGCTGAT GAATCAGAAT CCACGCCACA GGGAGACGCT CCAGCCACAC 
TGTAAGGAGC CTGACAACTC CCCACTCCCG GCAGACCGAA CTATTCGCAT AATCTCTCGT 
TCTGAGAATA ATCCAATGCC CCGGAGCAGC AGCAGTGTGG AGAGTGTGCC GATGTATTGC 
GTGAAGGAGC TGGAGTGTGA AGGTTCTGAA CTTTCCTTTG AGGAGCTCAG AGCGCGGCGG 
TACTTTAGCA AACGCCAACA GCTGGAGCTG CTGAGAAAAC AGGAGGAAGT GATATGGAAG 
CATGAGGAGG AAGAGGAGGA GGTGATAAGG ATGAAGAAGC TGCTGGAAGA GCTGAACAGC 
AAACTGAGCA CTGAACAACA GGAGACCAAA CACACATTCC TGCAGCAGGT TCCAGCTTCC 
AGACTGAGCC TGTCTCTGAG CGCAGCTTCA CAACTTGCAC ACAGCAGTGC ACTCAGTGCA 
GCTGTGAATA GGCCATTGGA CCTGGACACA CAGGCTCCAG TTCCACTACA GCTGCAAGAC 
CAGAGAACCG CAAGCGTGTG TGAGAGCCAG GCTGAGCGTT CGCTTCTAGA AAGTTGCTCT 
CAGAGGCTGG CAGTGTTTGA AAGAGACAGA TCTGAAAAAA CAGAGATGGA TGGAGGAGAT 
GCTAATGCAG ACAACGTGAT CATGGACATG TTCCAGGCTC CAACGCTGCT GCAGGACTCC 
GTCTTCAATA CTACCAGCCA GGTGGCTGAT GACAGCTTTG AGAAAAACTG CAGAGTAAAC 
GGTTTTGCCC CATCTGTAGC GAATCCACCT TCCTCAATGT CGTTCAGGAT ATACCAGGAT 
GAAAATGTGA AGATTGGAAG TGAGGAACAA CAGGTGGAGA AGAGCAAACC AGCAGCTCGA 
GCCCTGATGG AGATTCCGGT CTCCAAACCC AATGTCACTC CTGTAGGTCC GGAGTCTCTG 
ACAGACGAGA GTGCGATGTG GGGTCCTCAA TATGCCTCCA TAACTGCCTG TCCAAATCAT 
ACTCGTGATT TTGCCATGGC TGCCCACCTG GTATCCACCC CCCTCCACCC CACTGCCCCG 
TACACCTGGG ACACAGAGCT CAATCAGGTA GAAAACACTG CCAGAGGATT TAGTGGGACA 
GAGGAAAACC TGTATCAGCG CCAGCCAGCC AAACTGAGTC CAATCCTGGA GCAGAGTCCT 
TCTGATGAGA AGCCGTCTGA AGTTCCTGAG TGCAGTCTGA GGGCTCAGGG CACCATCGTA 
GGAGAGGGAG TGAGTCTTCC TCAGCAGAAC CAGACGCTGA CCCATGACCA GACCAGCTGC 
AGCATCGGCA GGCAGACGCT GGTCCCCCTG GCTTTACATG ATCACACTGC CGAGGCCAGC 
AGAGCCCTCA ACTCCAGCAC TAAACCTAGC TGGAGTGTCT ACCAGAGCCC AGAGAGAACC 
AGCCAGACAG GTTCTAACAC AGAACCTGTG TCCAACAGCA TGCTGTCTGT GAAACAGCAA 
ATACCTCACC AGCTCAATTC ATCTAATCTA CAGCTTAATT CCAGCTCTAG CATCAAACCC 
AGCTGGAGCA TCTACCAGAG CCCAGAGAAA ACCAGCCATA CGGGCTCGAA CAGAGGGCTC 
AACTCAAGCA GCATGCTGTC TGTAAGAGAG CCTCAACCGA TCCAATCCAA TGCAAATGGC 
CTACAGATGA ACTCTCTATC TTGCTCCCTT GCTGAGGCCA GCAATGCCCT CAACTCCAGT 
GCCAAACCTA ACTGGAGCAT CTACCAGAGC CCAGAGAAAA CCAGCCAGAC AGATTGTAAC 
ACAGAACCGA CCTCTAACAG CATACTGTCG GTAAAGCAGT ACCAAGCAGA CCAGTTCAAT 
TTACCTAGCA TGCAGGCTAA CTCCAGCTCC AGCACTAAAC CCAGCTGGAC CATCTACCAA 
AGCCCAGAGA AATCCCACCA GGCTAGTTTT TGTGTGGAAC CCAAACAACA CTCAAACAGC 
ATGCACTTGG AAGCGGGAGA ACCCAAACAG GACCAGTTCA ACTCAACTGG CTTGCAGGTT 
AATCCAGACA GAACCATGAA CTCCACCACC CAAAGCCCAG AGAGAACCCA AAAGACTCAA 
TTTAGTGGGG ACCTCCACTC AACCAGCATG CATTTAGTGG CAGAACCCAA ACAAGACCAG 
CTTAACTCAA CAGCCATTCA GCTGAACTCC AGCTGCAGCA CCAAACCCAG CTGGAGTATT 
TACCAGAGCC CAGAGAGAAC CAGTCAAATG GGTCACAGCA GGGAGTTCAA GGCAGACCAG 
CAGGTTAACT CAAACCAAGG GTTCAACTCT TCTAGCCTGC ATTTAATGGG AGAACTAAAA 
CCAGACCAAC TCAATTCCTC CATCAATATG TGTTCTGGTC AGGGTGAGGC TGATATTCTC 
TTGCCCAAGA AGTCTTTTCA CCGGCGAAAA TCCGAAAAAC AGCTGAACTT CGAGAGCCTT 
CAGGTGAGCC GACCTGTATC ACACCAGTCC TGCACGCTGC CAGAGAGCCC AAAACCACAG 
CCCAGAATAA TCCAGGATGT TCCCATGAGT CCAGACCCAA TCCCAGGGTC CGACTGGCTT 
CAGATGGACA GTCCAGTCCC GCTCTCTGAA CCCGACCTGG ATGTGATGGT CAGGTCGATC 
AGATCCCAGA CAATTCACAG AGAAACCCCC GGAGCTCCTG CTTCCAGGTC CAAACTCAGC 
TGGAGCGTGA ACCAGACCCC AGGGAGGCCA TCTGAACTAC AGTACGTCCC GCAGAGTCCT 
GAGCCCATGG AGGAATCAGA TCCGCATCAG TTATCAAAGC TGGAAGAGTC TGGACTTCTG 
CTCTCCAAGA GATCCTTTAA CAGGCGCAAA TCGGATAAGA GGGTATTGGA GAAATCCCTC 
TTGTCGAGTC AGAGGCAGTC CTCCAACCAA AACCAGGATG TTCCAATGAG TCCGGAACCA 
ATTTCGGGGC TGAACTGGCT CTGTCCAGAG AGTCCAGCCC GTGTGACTGA GTCTGATCTG 
GACGTCATGA TGACACCTCA GCAAAAATCT AAATCAGTGG ATGCTTCTGC CCAGCTGCTG 
CAGTGTGATG TTCCAATGAG CCCCCTGCAG GCTTCCATAC CTGATTACAC TCCCTCAGTG 
TTGGTGTCTG ACCCGTGGGA TGAGGACCTG ATAGATTCTT TACTCTCCAG CCTGAAGACG 
CCCCTCACCT CATACCCGAA CCTCAGCCTC TGCACTCGAG GCCTGCCCAC ACTCACCCCC 
AAGATGTCCG TACAGATGGG TGAGGACTCT CTGCGGGTGG ACTTTGTGTT GGGACAGGGT 
GCTTTCGCCA CCGTTTATCA GGCTACCAAC CTTACAACCT CACAAAAATT CATCCTAAAG 
GTGCAGAAGC CGGCGAACCC ATGGGAGTTT TACATCAACA GCCAGCTAAA TGCTCGAGTG 
CTGCGGAAAG AGCGTCACCT GTTCAACCAG CTCCACTCCG CTCACCTGTT CACCAATGGC 
AGTGTGCTTG TGGGGGAACT GCACAACTGC GGGACTCTGC TGAGTGTGGT GAATCTGTAT 
AAGAGCCGCA GTGAGAAGGT GATGCCCCAG CCGCTGGTGC TGTACTTCAG CATGTGTATC 
CTGCAGATGG TGGAGCAGCT GCATCAAGCC AAAATCATCC ACGCCGACAT CAAACCTGAC 
AACTTCCTGC TTGGAGAAAG GTTTCTGGAG AACGAGGATT TGGATGCTGA GAGTCTGGAT 
CACGGCTTGG TTCTGATAGA TTTTGGTCAA AGTATTGATA TGACCCTGTT CCCTGAGGGC 
ACGGCCTTCA CTGCCCGCTG CATGACTTCT GGCTTCCAGT GCACAGAGAT GCTGAGTGGC 
CGGCCGTGGA ACTACCAGAC TGATTACTTT GGCATTGCCG GCACCGTGCA CTGCATGATC 
TTCGGGTCAT ACATGCAAGT GAGAAATGAG GGTGGAGTTT GGAAAACGAA TGGCATTTTT 
AAAAGGAATC CTCACAGTGA TCTATGGCAG GAGTTTTTCC ATGTGCTGCT GAATGTTCCG 
GACTGTAACT CTGCCTTATG TTTGCGGAGT TTGCGTGAGC GTCTGTCTGT GGTGCTGCAG 
GAAAACTACA GCAACAAACT GCGCGGCCTT AAAAACCGAC TGGTGGTGCA GATCCTGGAG 
AGCCGCGGGG CTCGCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	bub1
Entrez Gene ID	103021907
Full Name	BUB1 mitotic checkpoint serine/threonine kinase
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

bub1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene

Clones

gRNAs

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