LOC725895 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC725895
Entrez Gene ID	725895
Full Name	multiple EGF-like-domains 8
Synonyms	Megf8
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_026439720.1	XP_026295505.1	multiple epidermal growth factor-like domains protein 8 isoform X1
XM_026439723.1	XP_026295508.1	multiple epidermal growth factor-like domains protein 8 isoform X1
XM_026439724.1	XP_026295509.1	multiple epidermal growth factor-like domains protein 8 isoform X2
XM_026439722.1	XP_026295507.1	multiple epidermal growth factor-like domains protein 8 isoform X1
XM_026439721.1	XP_026295506.1	multiple epidermal growth factor-like domains protein 8 isoform X1
XM_016911066.1	XP_016766555.1	LOW QUALITY PROTEIN: multiple epidermal growth factor-like domains protein 8

Pathways from BioSystems
Gene Ontology

The following LOC725895 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC725895 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHd21065	XM_016911066.1 Latest version!	Apis mellifera multiple EGF-like-domains 8 (LOC725895), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHd29707	XM_026439720.1 Latest version!	Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHd29707	XM_026439723.1 Latest version!	Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHd29707	XM_026439721.1 Latest version!	Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHd29707	XM_026439722.1 Latest version!	Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHd29708	XM_026439724.1 Latest version!	Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC725895 ( XM_016911066.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016766555.1 Apis mellifera multiple EGF-like-domains 8 (LOC725895), mRNA.

Price & Availability↑

CloneID	OHd21065
Clone ID Related Accession (Same CDS sequence)	XM_016911066.1
Accession Version	XM_016911066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463932800000
Organism	Apis mellifera(honey bee)
Product	LOW QUALITY PROTEIN: multiple epidermal growth factor-like domains protein 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007072.3) and transcript sequence (GAZV01023320.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel assembly gap :: added 333 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGCA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATGCA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAATAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTCTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTATGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTACAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTTTAT 
CATCAAGCTA CTAATTCTTT GTTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGATTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCANCTGAAA TAGTTCATAG TAAACAACAA 
ATATATTTTT GGAAATTTAG TCTACAAAAT CAAAGATGGA TGCAAATCAT ACAAGATAAG 
TTTAAAAAAG AACCACCATC TTTAGCTGGA CATACATTAA CTCTTCGTAG AAATGGAGAA 
TCAGAAAGTC TAATATTAAT TGGAGGATTC AGTCCTAAGT ATGGTTATCT TGACACAGTA 
TGGGAATTCA ATTTAGAAAC AGAAGCTTGG GATACAATAA ATACAATTGG AAATGGACCA 
TTAGGCGTAT ATGGCCATTC AACTGTATAT CATAGCAAGT CTGATAGTTT TTATATTTTC 
GGTGGATACA CTTATGCAAT AAATCGAACA TTTATTTCTA ATAAATTATA CGCGTTAAAT 
TATAAAACTC GTATGTGGTC CGTACTTCCT CCATTTGATG ATGATCTCAC TGATGGAAGT 
AGTTTGCCAC AAGCTAGATT TCTTCATTCT GCTGTTACCA CTGATGAATA TATGGTGATA 
TTTGGTGGCC GTCAAAATCC ACATAATACT TCAGACTCAT TGATTGCTTA TAAATATTCA 
TGTAATTTAT GGATTCGTTT AATAACAAAA GATATGGAAG TTATTGGATA TCCACCACCT 
CCAGCATATG CTCATGCAAT GACTCATGCT GATCCAGAAT CTAATGCTGT ATATGTAGTT 
GGTGGTTTTG ATGGTGGAAT TAAAAGTCAC GTCACTCTTA TAAGTATACC AGAAGATTTG 
TGTAATCTTT GGACAGATAA AATAACTTGC AGAAAATATT TTGGATGTTC TTTTTGTTCT 
GTTATTACAA TTAGTGGAAA TAATGCTTCA TTTTGTTTTT CTAATGAAAT ATCAATTAAT 
AAGGATGATA GATGTGATAT TAATGTTACT CAAGCTCAAC GATCAAATGG AATCTTTTGT 
AATTTAGAAT GGATGATGAG TAGAAAATGT CAAAATTTTA AAACTTGTAC AGAATGTTTA 
GCAGAATGGC CATATTATAA AAGTGAAGAA CCAGTGTGCA AATGGTGTAC AAATTGTTCA 
AATGGAAAGT GTATACCATC TGAGAAAGAT TGCGATGAAT TAAATAAATG CAGTGTTAGA 
CAAAAATCAG TAACAGATGT AAATCAATGT GGAGAACGTC AATGTCCAGC AAGTGATTGC 
GAGAAATGTA ACAATCTTGA AGGTTGTGTA TGGACAAGGC AGGTTTTGAA AACTTCTGAA 
TTGGGACTTG CAGTAACAGG AGAACCTGTC TATGATTGGA ATTGTGTACC AGAACATATT 
TTTGAAAGAT CAAGTATTAA AATGGGAAGT ACGCAATGTG AAAAACGATG TGGTGATCAT 
AAGGACTGTA AAAACTGTTT AAAAGGTACA GGAGCTGAAG GTGGTTGGCG AGAATGTAGA 
TGGTCAACGC AACTTAATGA ATGTATTTCT CCATCATATC AACCTCTGTA CTGTGCTGGC 
GGTGTATGTG GTTTGGTATT ACGTAGTTCT GATATGGATC ATTGTCCTGA ACCATGTTCA 
GTTTTTAAAC AATGCAGTAC ATGTTTGAAA CATTCACACT GTGGATGGTG TTCTTTAGAT 
TCAGCTAATA TAACTGGTCA AGGAATATGT ACAGAAGGTT CATTAGAAGC ACCAGCAGAT 
CATCCAGCTG GTGGAACATG TGAAATGTTA TATTATCAAC AGTTACCCAC AGTTGAACTA 
CTAAAGTTTC TGGAAATCTC AGACAATGTT ACAATGTTAA TTCCAAATGT GATGTCAAAA 
CCAATATTTT CTTGGCATTA TGTGCGATGC CCGCCAGAAA ATGAATGTAT GAATGGACAT 
CACACATGTT CCACAAAAAG TGAAAAATGT TTTGATTTAG AAGAAGGATT TGAATGCATG 
TGTGGCGACG GATATAAAAC TGAAACTCCA TCATCATTTA ATGAATTTGG AAGAAAAATT 
TGTGTTCCAA TGTGTACACA AGGTTGTGTT AGAGGAACAT GCATAGAACC AAATATTTGT 
CGTTGTGACT TCGGTTATGT AGGTGCCAAT TGTTCCATTC AGTGCCAATG TAATGGTCAT 
AGTAATTGTG CAGGCCCAGA TAAATTAGAC GTTTGTTTAG AATGTCATAA TAATACAATG 
GGACCACAAT GTGACAAATG TAGACCTTTA TTTGTTGGAA ATCCAGCAGA TAATGGTCAA 
TGTGTACCAT GTCTTGAATA TTGCAATGGA CATACTCGAA TATGTATTAA TGAAAGCATG 
ACTGTACCGG ATCCAAATTC AATTGAAAAA ATGTCTATAG AATTACTAGA AAAACAATTA 
GTCGAAGGTC CTGTAGCTAA AGCTAAATGC GTAAATTGTG GAAATAATAC AAGAGGTGAC 
AAATGTGGTG AATGTATGAT TGGTTATTTC AGAGGAACAG AAGATCTTCG TGATATATGC 
CGATCTTGTC AATGTCATGG ACATGGATTT ACTTGTGATC CAGTAACAGG AGAAAAATGC 
AATTGTGGTA ATAATACAGA AAGTGATCCA ACTTGTACTA GTGGACCTAT GAAAAGTACA 
AATACTGGTA GTTCCCCATG TTGGATGGTA CAATGTAGTA AATGTAAAGA AAATTATGCT 
GGGACACCAA CAATGGGTCA TCAATGTTAT AAAACTGTAA CTGTTGACAA CAAAATGTGC 
TTTGACTCTA AATTAATAGG AGGCTCTTAT GATGAGTGTA AAATGAAACC AAAACCATTG 
AATCCCGGTC AAACGGTATT TTATATGGTA CAGCCTCGTT TTATGAATGT TGATATTAGA 
GTTATGGTGG ATGTTACTCA AGGGGCTTTA GATCTATTTC TTAGCCCTCG AGATGATTCT 
TTCGTTGTTA CATTAAATTC AACAACAGGA TATCAAGATG TTGAACTGGA CAGTAGATTT 
ATGTGGCGAA AAAATTCACA TAATATGTGG TTAGATGAAC AAACTCGTAG TCAAATGAGA 
TTAGTTGAAT TCCAACCTCA TAATACTTTT TCATTTGCTT TAAATGGCAC TACAACTGAA 
CCGACTTGGA ATACTGGCCC TCAGTATATT GTAATGGAAA GATATGCAGA AGGTTTAGCT 
ACATTTTTAA CAATCGAGAA TAGAAATACA TTCTTGGTAG TTAGAAATCT TACAAATCGG 
TTAGTGTTGA CATTACCACA AGACAAACAT GAACTTCATG AAACAAAATT TCATATCGCA 
TTAAGAGCGA TTGATCCTGT GCATCCAGAA ATTAGTGGTC GAGCTGCTTA CGGGATGATT 
TTCTTTCGAC AGGATCAACT TCATATTGAT TTGTTTGTCT TTTTCTCTGT GTTCTTCTCT 
TGTTTCTTTT TATTTTTAGC CGGTTGTGTA GTTGCATGGA AAACGAAACA AGCAGCGGAT 
GTTCGAAGAG CACGGAGAAG ACATGTTGTC GAAATGTTGC ATATGGCTAA AAGACCTTTC 
GCATCAGCTA CCATCATTTA CGATCGAGAT GGTAATGACT GTAATCCAAG CTCTCCTCAG 
CGAAAAAGCA GACGTAATAA ATTAGTCAGC TTTCATAGTG ATGTTAGACC TGTAGCAGTT 
GAACCTACTG ATGATGGAGT TGCGGCTGTA GCCACTGTTT TCATTAGATT GCCTGGAGGT 
CGACAAGCTC CAGTTAAATT AGCTCTTGGT AGCTCATTAA TACTATTAAC TAGAGTTTAT 
CCAGTTAATA GTAGAGTGTT CCTAAGACGT AGAAACAGTC ACGCGTTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016766555.1
CDS	442..9495
Translation	MGWVLSTIIWITIWSCLLRGFELAPQISPKQIPCDKTRKVFTDSWGIISDGPLGSNYTQDSHCEWLIKANNSRQFITLSFRTMGTECSYDYVFVYDGDSFRSPLLGSFSGKTEPQQVTSSSGYMLILLYSDTNYVLDGFHAEFSVTNCSNNCTNHGKCIDNTCFCEDDWGGRDCSRALCPNNCSYSGKCDLKRCHCHNGYSGQSCSLHKMHPESNKWHWLSHSEGGLRPRAAHTAVYIKETDSLYVFGGYDLNYILDNLEVYRFNISQWEDEYGNELEGISSAEHLDPILLALELKGTNADEKELYGLRTSSFIWKLLSGIKDNNNTFNSRNLANVEKRYGWSEFRIGDREKKIHETKDNETSRSTVRIERNDDLKRKHSRHPRPRYAQTARHRRNLENLDFYKKFHTSNIKSDILNWEEEIAEDSIGDNIYVQEPKIRDELFKDESIKSSIDKLPKPSPRYGHAACRYDGGFVIYGGKVQDGSLSNELWYYNVNTRSWTLRAKNSPFYPPQLTRHTLTLANNYIYLFGGSTVDGEFSSNLYKIKLNLSDPTATTEKWKEVHPRGGKELDVRVVAHSTVYHQATNSLLIYGGVVASVARFSKLSDRMFVFQLDKKVWSEIHYPRAHLRDTYVPRERAFHTCNIIGNYLVVFGGYSHRHNKEEICYDNQMYLYHLGCHAWVSHDVLGLNDKDSRYPKQQGVFAHAADVRNGNTLLLVGGYHGNVNADLLAYTLPPMLAPGDEDNIEPEQICSRHKSLMECTANPECGWCSADEICYGRTIGSNCTTNLQTTRCLGVCPALGDCHSCLIHGQPGGGWGTTARGKKSVSNKLNRGTCTWCVQNARCHHKDDNYGVCGLRDDTLSQIPGWWGSKGTEIIKVEECREMDKRPGLTFLKYKPPVNFTQPDSVTIVNATTVDFSVPSMQGAKTESALGGEMIARLIGFLRPPQDFWDSTLEHLKICVSYNSATLHVSRSDDPQELELVANLTADTSQCIPTKWPDGYQMMLLPGRYLLDFESKRMVTASYAYASKMEIIHNKKTENPKVFTFEYLEPFQNGSCQQYKNCLHCLTDSSCGWCDIINKCLSRSINETESCIADVEWDEDGNPIREWHYLTITPSACANCSNYISCESCVNSNLCEWWTEEARCARIGRLPNAVVSFDQCPIPCRQRSNCTQCLDERGRCVWCEATQECFSFSVYTSEYQFGLCREWMDQAGLMGVTSRSGSSLTGNDQCKSCTRHTNCSSCLHSLSCGWCYSLENPIIGACVQGDFNQPHVNCSLIINEYQNTTLEIDESGWAYAQCPDVDECDLGLHDCHLNAVCTNTHGSFSCQCKRGFNGDGKENCTKTCYEKCVNGYCSEAPDYKCECNLGWTGPDCRTNCGCYNHSTCIQGPGICDKCQDWTTGTYCEECKAGSYGNATTPLGCKKCNCNEHGDKDLGICDRQTGICFCRDNTQGDMCQRCKKGYYGDPRDGGMCYYSCMSRGMLGGEGNGKQGLGSRHSQLSLWESHFGDSPTRECLWIVSPKTDLSSDNMLSGIQNVIQFTIHDDINVSCQENSVYVYDGLPEFVSSVTSHQSQLLGVYCTESTDYPVTVEAKSGFLTVHYKQLDEVEGFNASYLIMTCNNCPGNRECRNGNCLCKAGFVGINCDIEICPNNCTSTKKQGICDKGYGRCVCIPGYGGRDCSIKIKEHQLIFTELFNSEYLADHLDHLRKTLPRFGHTLVADRRGSLWMFGGYSLSHGPLNDIRLFDTKNNTWMPITVESTSKASMPQGRYFHAnullEIVHSKQQIYFWKFSLQNQRWMQIIQDKFKKEPPSLAGHTLTLRRNGESESLILIGGFSPKYGYLDTVWEFNLETEAWDTINTIGNGPLGVYGHSTVYHSKSDSFYIFGGYTYAINRTFISNKLYALNYKTRMWSVLPPFDDDLTDGSSLPQARFLHSAVTTDEYMVIFGGRQNPHNTSDSLIAYKYSCNLWIRLITKDMEVIGYPPPPAYAHAMTHADPESNAVYVVGGFDGGIKSHVTLISIPEDLCNLWTDKITCRKYFGCSFCSVITISGNNASFCFSNEISINKDDRCDINVTQAQRSNGIFCNLEWMMSRKCQNFKTCTECLAEWPYYKSEEPVCKWCTNCSNGKCIPSEKDCDELNKCSVRQKSVTDVNQCGERQCPASDCEKCNNLEGCVWTRQVLKTSELGLAVTGEPVYDWNCVPEHIFERSSIKMGSTQCEKRCGDHKDCKNCLKGTGAEGGWRECRWSTQLNECISPSYQPLYCAGGVCGLVLRSSDMDHCPEPCSVFKQCSTCLKHSHCGWCSLDSANITGQGICTEGSLEAPADHPAGGTCEMLYYQQLPTVELLKFLEISDNVTMLIPNVMSKPIFSWHYVRCPPENECMNGHHTCSTKSEKCFDLEEGFECMCGDGYKTETPSSFNEFGRKICVPMCTQGCVRGTCIEPNICRCDFGYVGANCSIQCQCNGHSNCAGPDKLDVCLECHNNTMGPQCDKCRPLFVGNPADNGQCVPCLEYCNGHTRICINESMTVPDPNSIEKMSIELLEKQLVEGPVAKAKCVNCGNNTRGDKCGECMIGYFRGTEDLRDICRSCQCHGHGFTCDPVTGEKCNCGNNTESDPTCTSGPMKSTNTGSSPCWMVQCSKCKENYAGTPTMGHQCYKTVTVDNKMCFDSKLIGGSYDECKMKPKPLNPGQTVFYMVQPRFMNVDIRVMVDVTQGALDLFLSPRDDSFVVTLNSTTGYQDVELDSRFMWRKNSHNMWLDEQTRSQMRLVEFQPHNTFSFALNGTTTEPTWNTGPQYIVMERYAEGLATFLTIENRNTFLVVRNLTNRLVLTLPQDKHELHETKFHIALRAIDPVHPEISGRAAYGMIFFRQDQLHIDLFVFFSVFFSCFFLFLAGCVVAWKTKQAADVRRARRRHVVEMLHMAKRPFASATIIYDRDGNDCNPSSPQRKSRRNKLVSFHSDVRPVAVEPTDDGVAAVATVFIRLPGGRQAPVKLALGSSLILLTRVYPVNSRVFLRRRNSHALN

Target ORF information:

RefSeq Version	XM_016911066.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera multiple EGF-like-domains 8 (LOC725895), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016911066.1

ORF Insert Sequence:

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGCA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATGCA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAATAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTCTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTATGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTACAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTTTAT 
CATCAAGCTA CTAATTCTTT GTTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGATTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCANCTGAAA TAGTTCATAG TAAACAACAA 
ATATATTTTT GGAAATTTAG TCTACAAAAT CAAAGATGGA TGCAAATCAT ACAAGATAAG 
TTTAAAAAAG AACCACCATC TTTAGCTGGA CATACATTAA CTCTTCGTAG AAATGGAGAA 
TCAGAAAGTC TAATATTAAT TGGAGGATTC AGTCCTAAGT ATGGTTATCT TGACACAGTA 
TGGGAATTCA ATTTAGAAAC AGAAGCTTGG GATACAATAA ATACAATTGG AAATGGACCA 
TTAGGCGTAT ATGGCCATTC AACTGTATAT CATAGCAAGT CTGATAGTTT TTATATTTTC 
GGTGGATACA CTTATGCAAT AAATCGAACA TTTATTTCTA ATAAATTATA CGCGTTAAAT 
TATAAAACTC GTATGTGGTC CGTACTTCCT CCATTTGATG ATGATCTCAC TGATGGAAGT 
AGTTTGCCAC AAGCTAGATT TCTTCATTCT GCTGTTACCA CTGATGAATA TATGGTGATA 
TTTGGTGGCC GTCAAAATCC ACATAATACT TCAGACTCAT TGATTGCTTA TAAATATTCA 
TGTAATTTAT GGATTCGTTT AATAACAAAA GATATGGAAG TTATTGGATA TCCACCACCT 
CCAGCATATG CTCATGCAAT GACTCATGCT GATCCAGAAT CTAATGCTGT ATATGTAGTT 
GGTGGTTTTG ATGGTGGAAT TAAAAGTCAC GTCACTCTTA TAAGTATACC AGAAGATTTG 
TGTAATCTTT GGACAGATAA AATAACTTGC AGAAAATATT TTGGATGTTC TTTTTGTTCT 
GTTATTACAA TTAGTGGAAA TAATGCTTCA TTTTGTTTTT CTAATGAAAT ATCAATTAAT 
AAGGATGATA GATGTGATAT TAATGTTACT CAAGCTCAAC GATCAAATGG AATCTTTTGT 
AATTTAGAAT GGATGATGAG TAGAAAATGT CAAAATTTTA AAACTTGTAC AGAATGTTTA 
GCAGAATGGC CATATTATAA AAGTGAAGAA CCAGTGTGCA AATGGTGTAC AAATTGTTCA 
AATGGAAAGT GTATACCATC TGAGAAAGAT TGCGATGAAT TAAATAAATG CAGTGTTAGA 
CAAAAATCAG TAACAGATGT AAATCAATGT GGAGAACGTC AATGTCCAGC AAGTGATTGC 
GAGAAATGTA ACAATCTTGA AGGTTGTGTA TGGACAAGGC AGGTTTTGAA AACTTCTGAA 
TTGGGACTTG CAGTAACAGG AGAACCTGTC TATGATTGGA ATTGTGTACC AGAACATATT 
TTTGAAAGAT CAAGTATTAA AATGGGAAGT ACGCAATGTG AAAAACGATG TGGTGATCAT 
AAGGACTGTA AAAACTGTTT AAAAGGTACA GGAGCTGAAG GTGGTTGGCG AGAATGTAGA 
TGGTCAACGC AACTTAATGA ATGTATTTCT CCATCATATC AACCTCTGTA CTGTGCTGGC 
GGTGTATGTG GTTTGGTATT ACGTAGTTCT GATATGGATC ATTGTCCTGA ACCATGTTCA 
GTTTTTAAAC AATGCAGTAC ATGTTTGAAA CATTCACACT GTGGATGGTG TTCTTTAGAT 
TCAGCTAATA TAACTGGTCA AGGAATATGT ACAGAAGGTT CATTAGAAGC ACCAGCAGAT 
CATCCAGCTG GTGGAACATG TGAAATGTTA TATTATCAAC AGTTACCCAC AGTTGAACTA 
CTAAAGTTTC TGGAAATCTC AGACAATGTT ACAATGTTAA TTCCAAATGT GATGTCAAAA 
CCAATATTTT CTTGGCATTA TGTGCGATGC CCGCCAGAAA ATGAATGTAT GAATGGACAT 
CACACATGTT CCACAAAAAG TGAAAAATGT TTTGATTTAG AAGAAGGATT TGAATGCATG 
TGTGGCGACG GATATAAAAC TGAAACTCCA TCATCATTTA ATGAATTTGG AAGAAAAATT 
TGTGTTCCAA TGTGTACACA AGGTTGTGTT AGAGGAACAT GCATAGAACC AAATATTTGT 
CGTTGTGACT TCGGTTATGT AGGTGCCAAT TGTTCCATTC AGTGCCAATG TAATGGTCAT 
AGTAATTGTG CAGGCCCAGA TAAATTAGAC GTTTGTTTAG AATGTCATAA TAATACAATG 
GGACCACAAT GTGACAAATG TAGACCTTTA TTTGTTGGAA ATCCAGCAGA TAATGGTCAA 
TGTGTACCAT GTCTTGAATA TTGCAATGGA CATACTCGAA TATGTATTAA TGAAAGCATG 
ACTGTACCGG ATCCAAATTC AATTGAAAAA ATGTCTATAG AATTACTAGA AAAACAATTA 
GTCGAAGGTC CTGTAGCTAA AGCTAAATGC GTAAATTGTG GAAATAATAC AAGAGGTGAC 
AAATGTGGTG AATGTATGAT TGGTTATTTC AGAGGAACAG AAGATCTTCG TGATATATGC 
CGATCTTGTC AATGTCATGG ACATGGATTT ACTTGTGATC CAGTAACAGG AGAAAAATGC 
AATTGTGGTA ATAATACAGA AAGTGATCCA ACTTGTACTA GTGGACCTAT GAAAAGTACA 
AATACTGGTA GTTCCCCATG TTGGATGGTA CAATGTAGTA AATGTAAAGA AAATTATGCT 
GGGACACCAA CAATGGGTCA TCAATGTTAT AAAACTGTAA CTGTTGACAA CAAAATGTGC 
TTTGACTCTA AATTAATAGG AGGCTCTTAT GATGAGTGTA AAATGAAACC AAAACCATTG 
AATCCCGGTC AAACGGTATT TTATATGGTA CAGCCTCGTT TTATGAATGT TGATATTAGA 
GTTATGGTGG ATGTTACTCA AGGGGCTTTA GATCTATTTC TTAGCCCTCG AGATGATTCT 
TTCGTTGTTA CATTAAATTC AACAACAGGA TATCAAGATG TTGAACTGGA CAGTAGATTT 
ATGTGGCGAA AAAATTCACA TAATATGTGG TTAGATGAAC AAACTCGTAG TCAAATGAGA 
TTAGTTGAAT TCCAACCTCA TAATACTTTT TCATTTGCTT TAAATGGCAC TACAACTGAA 
CCGACTTGGA ATACTGGCCC TCAGTATATT GTAATGGAAA GATATGCAGA AGGTTTAGCT 
ACATTTTTAA CAATCGAGAA TAGAAATACA TTCTTGGTAG TTAGAAATCT TACAAATCGG 
TTAGTGTTGA CATTACCACA AGACAAACAT GAACTTCATG AAACAAAATT TCATATCGCA 
TTAAGAGCGA TTGATCCTGT GCATCCAGAA ATTAGTGGTC GAGCTGCTTA CGGGATGATT 
TTCTTTCGAC AGGATCAACT TCATATTGAT TTGTTTGTCT TTTTCTCTGT GTTCTTCTCT 
TGTTTCTTTT TATTTTTAGC CGGTTGTGTA GTTGCATGGA AAACGAAACA AGCAGCGGAT 
GTTCGAAGAG CACGGAGAAG ACATGTTGTC GAAATGTTGC ATATGGCTAA AAGACCTTTC 
GCATCAGCTA CCATCATTTA CGATCGAGAT GGTAATGACT GTAATCCAAG CTCTCCTCAG 
CGAAAAAGCA GACGTAATAA ATTAGTCAGC TTTCATAGTG ATGTTAGACC TGTAGCAGTT 
GAACCTACTG ATGATGGAGT TGCGGCTGTA GCCACTGTTT TCATTAGATT GCCTGGAGGT 
CGACAAGCTC CAGTTAAATT AGCTCTTGGT AGCTCATTAA TACTATTAAC TAGAGTTTAT 
CCAGTTAATA GTAGAGTGTT CCTAAGACGT AGAAACAGTC ACGCGTTGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC725895 ( XM_026439720.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026295505.1 Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X1, mRNA.

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CloneID	OHd29707
Clone ID Related Accession (Same CDS sequence)	XM_026439720.1 , XM_026439723.1 , XM_026439722.1 , XM_026439721.1
Accession Version	XM_026439720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	multiple epidermal growth factor-like domains protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037640.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGTA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATACA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAACAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTTTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTACGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTATAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTATAT 
CATCAAGCTA CTAATTCTTT ATTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGACTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCAACTGAAA TAGTTCATAG TAAACAACAA 
ATATATATTT ATGGTGGCTT GACAATGAAA CAAGAAGATA TTCAAGGTTT ATCAAATAAC 
ACTCTAAACG ATTTTTGGAA ATTTAGTCTA CAAAATCAAA GATGGATGCA AATCATACAA 
GATAAGTTTA AAAAAGAACC ACCATCTTTA GCTGGACATA CATTAACTCT TCGTAGAAAT 
GGAGAATCAG AAAGTCTAAT ATTAATTGGA GGATTCAGTC CTAAGTATGG TTATCTTGAC 
ACAGTATGGG AATTCAATTT AGAAACAGAA GCTTGGGATA CAATAAATAC AATTGGAAAT 
GGACCATTAG GCGTATATGG CCATTCAACT GTATATCATA GCAAGTCTGA TAGTTTTTAT 
ATTTTCGGTG GATACACTTA TGCGATAAAT CGAACATTTA TTTCTAATAA ATTATACGCA 
TTGAATTATA AAACTCGTAT GTGGTCCGTA CTTCCTCCAT TTGATGATGA TCTCACTGAT 
GGAAGTAGTT TGCCACAAGC TAGATTTCTT CATTCTGCTG TTACCACTGA TGAATATATG 
GTGATATTTG GTGGCCGTCA AAATCCACAT AATACTTCAG ACTCATTGAT TGCTTATAAA 
TATTCATGTA ATTTATGGAT TCGTTTAATA ACAAAAGATA TGGAAGTTAT TGGATATCCA 
CCACCTCCAG CATATGCTCA TGCAATGACT CATGCTGATC CAGAATCTAA TGCTGTATAT 
GTAGTTGGTG GTTTTGATGG TGGAATTAAA AGTCACGTCA CTCTTATAAG TATACCAGAA 
GATTTGTGTA ATCTTTGGAC AGATAAAATA ACTTGCAGAA AATATTTTGG ATGTTCTTTT 
TGTTCTGTTA TTACAATTAG TGGAAATAAT GCTTCATTTT GTTTTTCTAA TGAAATATCA 
ATTAATAAGG ATGATAGATG TGATATTAAT GTTACTCAAG CTCAACGATC AAATGGAATC 
TTTTGTAATT TAGAATGGAT GATGAGTAGA AAATGTCAAA ATTTTAAAAC TTGTACAGAA 
TGTTTAGCAG AATGGCCATA TTATAAAAGT GAAGAACCAG TGTGCAAATG GTGTACAAAT 
TGTTCAAATG GAAAGTGTAT ACCATCTGAG AAAGATTGCG ATGAATTAAA TAAATGCAGT 
GTTAGACAAA AATCAGTAAC AGATGTAAAT CAATGTGGAG AACGTCAATG TCCAGCAAGT 
GATTGCGAGA AATGTAACAA TCTTGAAGGT TGTGTATGGA CAAGGCAGGT TTTGAAAACT 
TCTGAATTGG GACTTGCAGT AACAGGAGAA CCTGTCTATG ATTGGAATTG TGTACCAGAA 
CATATTTTTG AAAGATCAAG TATTAAAATG GGAAGTACGC AATGTGAAAA ACGATGTGGT 
GATCATAAGG ACTGTAAAAA CTGTTTAAAA GGTACAGGAG CTGAAGGTGG TTGGCGAGAA 
TGTAGATGGT CAACGCAACT TAATGAATGT ATTTCTCCAT CATATCAACC TCTGTACTGT 
GCTGGCGGTG TATGTGGTTT GGTATTACGT AGTTCTGATA TGGATCATTG TCCTGAACCA 
TGTTCAGTTT TTAAACAATG CAGTACATGT TTGAAACATT CACACTGTGG ATGGTGTTCT 
TTAGATTCAG CTAATATAAC TGGTCAAGGA ATATGTACAG AAGGTTCATT AGAAGCACCA 
GCAGATCATC CAGCTGGTGG AACATGTGAA ATGTTATATT ATCAACAGTT ACCCACAGTT 
GAACTACTAA AGTTTCTGGA AATCTCAGAC AATGTTACAA TGTTAATTCC AAACGTGATG 
TCAAAACCAA TATTTTCTTG GCATTATGTA CGATGCCCGC CAGAAAATGA ATGTATGAAT 
GGACACCACA CATGTTCCAC AAAAAGTGAA AAATGTTTTG ATTTAGAAGA AGGATTTGAA 
TGCATGTGTG GCGACGGATA TAAAACTGAA ACTCCATCAT CATTTAATGA ATTTGGAAGA 
AAAATTTGCG TTCCAATGTG TACACAAGGT TGTGTTAGAG GAACATGCAT AGAACCAAAT 
ATTTGTCGTT GTGACTTTGG TTATGTAGGT GCCAATTGTT CCATTCAGTG CCAATGTAAT 
GGTCATAGTA ATTGTGCAGG CCCAGATAAA TTAGACGTTT GTTTAGAATG TCATAATAAT 
ACAATGGGAC CACAATGTGA CAAATGTAGA CCTTTATTTG TTGGAAATCC AGCAGATAAT 
GGTCAATGTG TACCATGTCT TGAATATTGC AATGGACATA CTCGAATATG TATTAATGAA 
AGCATGACTG TACCGGATCC AAATTCAATT GAAAAAATGT CTATAGAATT ACTAGAAAAA 
CAATTAGTCG AAGGTCCTGT AGCTAAAGCT AAATGCGTAA ATTGTGGAAA TAATACAAGA 
GGTGACAAAT GTGGTGAATG TATGATTGGT TATTTCAGAG GAACAGAAGA TCTTCGTGAT 
ATATGCCGAT CTTGTCAATG TCATGGACAT GGATTTACTT GTGATCCAGT AACAGGAGAA 
AAATGCAATT GTGGTAATAA TACAGAAAGT GATCCAACTT GTACTAGTGG ACCTATGAAA 
AGTACAAATA CTGGTAGTTC CCCATGTTGG ATGGTACAAT GTAGTAAATG TAAAGAAAAT 
TATGCTGGGA CACCAACAAT GGGTCATCAA TGTTATAAAA CTGTAACTGT TGACAACAAA 
ATGTGCTTTG ACTCTAAATT AATAGGAGGC TCTTATGATG AGTGTAAAAT GAAACCAAAA 
CCATTGAATC CCGGTCAAAC GGTATTTTAT ATGGTACAGC CTCGTTTTAT GAATGTTGAT 
ATTAGAGTTA TGGTGGATGT TACTCAAGGG GCTTTAGATC TATTTCTTAG CCCTCGAGAT 
GATTCTTTCG TTGTTACATT AAATTCAACA ACAGGATATC AAGATGTTGA ACTGGACAGT 
AGATTTATGT GGCGAAAAAA TTCACATAAT ATGTGGTTAG ATGAACAAAC TCGTAGTCAA 
ATGAGATTAG TTGAATTCCA ACCTCATAAT ACTTTTTCAT TTGCTTTAAA TGGCACTACA 
ACTGAACCGA CTTGGAATAC TGGCCCTCAG TATATTGTAA TGGAAAGATA TGCAGAAGGT 
TTAGCTACAT TTTTAACAAT CGAGAATAGA AATACATTCT TGGTAGTTAG AAATCTTACA 
AATCGGTTAG TGTTGACATT ACCACAAGAC AAACATGAAC TTCATGAAAC AAAATTTCAT 
ATCGCATTAA GAGCGATTGA TCCTGTGCAT CCAGAAATTA GTGGTCGAGC TGCTTACGGG 
ATGATTTTCT TTCGACAGGA TCAACTTCAT ATTGATTTGT TTGTCTTTTT CTCTGTGTTC 
TTCTCTTGTT TCTTTTTATT TTTAGCCGGT TGTGTAGTTG CATGGAAAAC GAAACAAGCA 
GCGGATGTTC GAAGAGCACG GAGAAGACAT GTTGTCGAAA TGTTGCATAT GGCTAAAAGA 
CCTTTCGCAT CAGCTACCAT CATTTACGAT CGAGATGGTA ATGACTGTAA TCCAAGCTCT 
CCTCAGCGAA AAAGCAGACG TAATAAATTA GTCAGCTTTC ATAGTGATGT TAGACCTGTA 
GCAGTTGAAC CTACTGATGA TGGAGTTGCG GCTGTAGCCA CTGTTTTCAT TAGATTGCCT 
GGAGGTCGAC AAGCTCCAGT TAAATTAGCT CTTGGTAGCT CATTAATACT ATTAACTAGA 
GTTTATCCAG TTAATAGTAG AGTGTTCCTA AGACGTAGAA ACAGTCACGC GTTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026295505.1
CDS	434..9553
Translation	MGWVLSTIIWITIWSCLLRGFELAPQISPKQIPCDKTRKVFTDSWGIISDGPLGSNYTQDSHCEWLIKANNSRQFITLSFRTMGTECSYDYVFVYDGDSFRSPLLGSFSGKTEPQQVTSSSGYMLILLYSDTNYVLDGFHAEFSVTNCSNNCTNHGKCIDNTCFCEDDWGGRDCSRALCPNNCSYSGKCDLKRCHCHNGYSGQSCSLHKMHPESNKWHWLSHSEGGLRPRAAHTAVYIKETDSLYVFGGYDLNYILDNLEVYRFNISQWEDEYGNELEGISSAEHLDPILLALELKGTNTDEKELYGLRTSSFIWKLLSGIKDNNNTFNSRNLANVEKRYGWSEFRIGDREKKIHETKDNETSRSTVRIERNDDLKRKHSRHPRPRYAQTARHRRNLENLDFYKKFHTSNIKSDILNWEEEIAEDSIGDNIYVQEPKIRDELFKDESIKSSIDKLPKPSPRYGHAACRYDGGFVIYGGKVQDGSLSNELWYYNVNTRSWTLRAKNSPFYPPQLTRHTLTLANNYIYLFGGSTVDGEFSSNLYKIKLNLSDPTATTEKWKEVHPRGGKELDVRVVAHSTVYHQATNSLLIYGGVVASVARFSKLSDRMFVFQLDKKVWSEIHYPRAHLRDTYVPRERAFHTCNIIGNYLVVFGGYSHRHNKEEICYDNQMYLYHLGCHAWVSHDVLGLNDKDSRYPKQQGVFAHAADVRNGNTLLLVGGYHGNVNADLLAYTLPPMLAPGDEDNIEPEQICSRHKSLMECTANPECGWCSADEICYGRTIGSNCTTNLQTTRCLGVCPALGDCHSCLIHGQPGGGWGTTARGKKSVSNKLNRGTCTWCVQNARCHHKDDNYGVCGLRDDTLSQIPGWWGSKGTEIIKVEECREMDKRPGLTFLKYKPPVNFTQPDSVTIVNATTVDFSVPSMQGAKTESALGGEMIARLIGFLRPPQDFWDSTLEHLKICVSYNSATLHVSRSDDPQELELVANLTADTSQCIPTKWPDGYQMMLLPGRYLLDFESKRMVTASYAYASKMEIIHNKKTENPKVFTFEYLEPFQNGSCQQYKNCLHCLTDSSCGWCDIINKCLSRSINETESCIADVEWDEDGNPIREWHYLTITPSACANCSNYISCESCVNSNLCEWWTEEARCARIGRLPNAVVSFDQCPIPCRQRSNCTQCLDERGRCVWCEATQECFSFSVYTSEYQFGLCREWMDQAGLMGVTSRSGSSLTGNDQCKSCTRHTNCSSCLHSLSCGWCYSLENPIIGACVQGDFNQPHVNCSLIINEYQNTTLEIDESGWAYAQCPDVDECDLGLHDCHLNAVCTNTHGSFSCQCKRGFNGDGKENCTKTCYEKCVNGYCSEAPDYKCECNLGWTGPDCRTNCGCYNHSTCIQGPGICDKCQDWTTGTYCEECKAGSYGNATTPLGCKKCNCNEHGDKDLGICDRQTGICFCRDNTQGDMCQRCKKGYYGDPRDGGMCYYSCMSRGMLGGEGNGKQGLGSRHSQLSLWESHFGDSPTRECLWIVSPKTDLSSDNMLSGIQNVIQFTIHDDINVSCQENSVYVYDGLPEFVSSVTSHQSQLLGVYCTESTDYPVTVEAKSGFLTVHYKQLDEVEGFNASYLIMTCNNCPGNRECRNGNCLCKAGFVGINCDIEICPNNCTSTKKQGICDKGYGRCVCIPGYGGRDCSIKIKEHQLIFTELFNSEYLADHLDHLRKTLPRFGHTLVADRRGSLWMFGGYSLSHGPLNDIRLFDTKNNTWMPITVESTSKASMPQGRYFHATEIVHSKQQIYIYGGLTMKQEDIQGLSNNTLNDFWKFSLQNQRWMQIIQDKFKKEPPSLAGHTLTLRRNGESESLILIGGFSPKYGYLDTVWEFNLETEAWDTINTIGNGPLGVYGHSTVYHSKSDSFYIFGGYTYAINRTFISNKLYALNYKTRMWSVLPPFDDDLTDGSSLPQARFLHSAVTTDEYMVIFGGRQNPHNTSDSLIAYKYSCNLWIRLITKDMEVIGYPPPPAYAHAMTHADPESNAVYVVGGFDGGIKSHVTLISIPEDLCNLWTDKITCRKYFGCSFCSVITISGNNASFCFSNEISINKDDRCDINVTQAQRSNGIFCNLEWMMSRKCQNFKTCTECLAEWPYYKSEEPVCKWCTNCSNGKCIPSEKDCDELNKCSVRQKSVTDVNQCGERQCPASDCEKCNNLEGCVWTRQVLKTSELGLAVTGEPVYDWNCVPEHIFERSSIKMGSTQCEKRCGDHKDCKNCLKGTGAEGGWRECRWSTQLNECISPSYQPLYCAGGVCGLVLRSSDMDHCPEPCSVFKQCSTCLKHSHCGWCSLDSANITGQGICTEGSLEAPADHPAGGTCEMLYYQQLPTVELLKFLEISDNVTMLIPNVMSKPIFSWHYVRCPPENECMNGHHTCSTKSEKCFDLEEGFECMCGDGYKTETPSSFNEFGRKICVPMCTQGCVRGTCIEPNICRCDFGYVGANCSIQCQCNGHSNCAGPDKLDVCLECHNNTMGPQCDKCRPLFVGNPADNGQCVPCLEYCNGHTRICINESMTVPDPNSIEKMSIELLEKQLVEGPVAKAKCVNCGNNTRGDKCGECMIGYFRGTEDLRDICRSCQCHGHGFTCDPVTGEKCNCGNNTESDPTCTSGPMKSTNTGSSPCWMVQCSKCKENYAGTPTMGHQCYKTVTVDNKMCFDSKLIGGSYDECKMKPKPLNPGQTVFYMVQPRFMNVDIRVMVDVTQGALDLFLSPRDDSFVVTLNSTTGYQDVELDSRFMWRKNSHNMWLDEQTRSQMRLVEFQPHNTFSFALNGTTTEPTWNTGPQYIVMERYAEGLATFLTIENRNTFLVVRNLTNRLVLTLPQDKHELHETKFHIALRAIDPVHPEISGRAAYGMIFFRQDQLHIDLFVFFSVFFSCFFLFLAGCVVAWKTKQAADVRRARRRHVVEMLHMAKRPFASATIIYDRDGNDCNPSSPQRKSRRNKLVSFHSDVRPVAVEPTDDGVAAVATVFIRLPGGRQAPVKLALGSSLILLTRVYPVNSRVFLRRRNSHALN

Target ORF information:

RefSeq Version	XM_026439720.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026439720.1

ORF Insert Sequence:

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGTA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATACA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAACAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTTTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTACGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTATAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTATAT 
CATCAAGCTA CTAATTCTTT ATTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGACTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCAACTGAAA TAGTTCATAG TAAACAACAA 
ATATATATTT ATGGTGGCTT GACAATGAAA CAAGAAGATA TTCAAGGTTT ATCAAATAAC 
ACTCTAAACG ATTTTTGGAA ATTTAGTCTA CAAAATCAAA GATGGATGCA AATCATACAA 
GATAAGTTTA AAAAAGAACC ACCATCTTTA GCTGGACATA CATTAACTCT TCGTAGAAAT 
GGAGAATCAG AAAGTCTAAT ATTAATTGGA GGATTCAGTC CTAAGTATGG TTATCTTGAC 
ACAGTATGGG AATTCAATTT AGAAACAGAA GCTTGGGATA CAATAAATAC AATTGGAAAT 
GGACCATTAG GCGTATATGG CCATTCAACT GTATATCATA GCAAGTCTGA TAGTTTTTAT 
ATTTTCGGTG GATACACTTA TGCGATAAAT CGAACATTTA TTTCTAATAA ATTATACGCA 
TTGAATTATA AAACTCGTAT GTGGTCCGTA CTTCCTCCAT TTGATGATGA TCTCACTGAT 
GGAAGTAGTT TGCCACAAGC TAGATTTCTT CATTCTGCTG TTACCACTGA TGAATATATG 
GTGATATTTG GTGGCCGTCA AAATCCACAT AATACTTCAG ACTCATTGAT TGCTTATAAA 
TATTCATGTA ATTTATGGAT TCGTTTAATA ACAAAAGATA TGGAAGTTAT TGGATATCCA 
CCACCTCCAG CATATGCTCA TGCAATGACT CATGCTGATC CAGAATCTAA TGCTGTATAT 
GTAGTTGGTG GTTTTGATGG TGGAATTAAA AGTCACGTCA CTCTTATAAG TATACCAGAA 
GATTTGTGTA ATCTTTGGAC AGATAAAATA ACTTGCAGAA AATATTTTGG ATGTTCTTTT 
TGTTCTGTTA TTACAATTAG TGGAAATAAT GCTTCATTTT GTTTTTCTAA TGAAATATCA 
ATTAATAAGG ATGATAGATG TGATATTAAT GTTACTCAAG CTCAACGATC AAATGGAATC 
TTTTGTAATT TAGAATGGAT GATGAGTAGA AAATGTCAAA ATTTTAAAAC TTGTACAGAA 
TGTTTAGCAG AATGGCCATA TTATAAAAGT GAAGAACCAG TGTGCAAATG GTGTACAAAT 
TGTTCAAATG GAAAGTGTAT ACCATCTGAG AAAGATTGCG ATGAATTAAA TAAATGCAGT 
GTTAGACAAA AATCAGTAAC AGATGTAAAT CAATGTGGAG AACGTCAATG TCCAGCAAGT 
GATTGCGAGA AATGTAACAA TCTTGAAGGT TGTGTATGGA CAAGGCAGGT TTTGAAAACT 
TCTGAATTGG GACTTGCAGT AACAGGAGAA CCTGTCTATG ATTGGAATTG TGTACCAGAA 
CATATTTTTG AAAGATCAAG TATTAAAATG GGAAGTACGC AATGTGAAAA ACGATGTGGT 
GATCATAAGG ACTGTAAAAA CTGTTTAAAA GGTACAGGAG CTGAAGGTGG TTGGCGAGAA 
TGTAGATGGT CAACGCAACT TAATGAATGT ATTTCTCCAT CATATCAACC TCTGTACTGT 
GCTGGCGGTG TATGTGGTTT GGTATTACGT AGTTCTGATA TGGATCATTG TCCTGAACCA 
TGTTCAGTTT TTAAACAATG CAGTACATGT TTGAAACATT CACACTGTGG ATGGTGTTCT 
TTAGATTCAG CTAATATAAC TGGTCAAGGA ATATGTACAG AAGGTTCATT AGAAGCACCA 
GCAGATCATC CAGCTGGTGG AACATGTGAA ATGTTATATT ATCAACAGTT ACCCACAGTT 
GAACTACTAA AGTTTCTGGA AATCTCAGAC AATGTTACAA TGTTAATTCC AAACGTGATG 
TCAAAACCAA TATTTTCTTG GCATTATGTA CGATGCCCGC CAGAAAATGA ATGTATGAAT 
GGACACCACA CATGTTCCAC AAAAAGTGAA AAATGTTTTG ATTTAGAAGA AGGATTTGAA 
TGCATGTGTG GCGACGGATA TAAAACTGAA ACTCCATCAT CATTTAATGA ATTTGGAAGA 
AAAATTTGCG TTCCAATGTG TACACAAGGT TGTGTTAGAG GAACATGCAT AGAACCAAAT 
ATTTGTCGTT GTGACTTTGG TTATGTAGGT GCCAATTGTT CCATTCAGTG CCAATGTAAT 
GGTCATAGTA ATTGTGCAGG CCCAGATAAA TTAGACGTTT GTTTAGAATG TCATAATAAT 
ACAATGGGAC CACAATGTGA CAAATGTAGA CCTTTATTTG TTGGAAATCC AGCAGATAAT 
GGTCAATGTG TACCATGTCT TGAATATTGC AATGGACATA CTCGAATATG TATTAATGAA 
AGCATGACTG TACCGGATCC AAATTCAATT GAAAAAATGT CTATAGAATT ACTAGAAAAA 
CAATTAGTCG AAGGTCCTGT AGCTAAAGCT AAATGCGTAA ATTGTGGAAA TAATACAAGA 
GGTGACAAAT GTGGTGAATG TATGATTGGT TATTTCAGAG GAACAGAAGA TCTTCGTGAT 
ATATGCCGAT CTTGTCAATG TCATGGACAT GGATTTACTT GTGATCCAGT AACAGGAGAA 
AAATGCAATT GTGGTAATAA TACAGAAAGT GATCCAACTT GTACTAGTGG ACCTATGAAA 
AGTACAAATA CTGGTAGTTC CCCATGTTGG ATGGTACAAT GTAGTAAATG TAAAGAAAAT 
TATGCTGGGA CACCAACAAT GGGTCATCAA TGTTATAAAA CTGTAACTGT TGACAACAAA 
ATGTGCTTTG ACTCTAAATT AATAGGAGGC TCTTATGATG AGTGTAAAAT GAAACCAAAA 
CCATTGAATC CCGGTCAAAC GGTATTTTAT ATGGTACAGC CTCGTTTTAT GAATGTTGAT 
ATTAGAGTTA TGGTGGATGT TACTCAAGGG GCTTTAGATC TATTTCTTAG CCCTCGAGAT 
GATTCTTTCG TTGTTACATT AAATTCAACA ACAGGATATC AAGATGTTGA ACTGGACAGT 
AGATTTATGT GGCGAAAAAA TTCACATAAT ATGTGGTTAG ATGAACAAAC TCGTAGTCAA 
ATGAGATTAG TTGAATTCCA ACCTCATAAT ACTTTTTCAT TTGCTTTAAA TGGCACTACA 
ACTGAACCGA CTTGGAATAC TGGCCCTCAG TATATTGTAA TGGAAAGATA TGCAGAAGGT 
TTAGCTACAT TTTTAACAAT CGAGAATAGA AATACATTCT TGGTAGTTAG AAATCTTACA 
AATCGGTTAG TGTTGACATT ACCACAAGAC AAACATGAAC TTCATGAAAC AAAATTTCAT 
ATCGCATTAA GAGCGATTGA TCCTGTGCAT CCAGAAATTA GTGGTCGAGC TGCTTACGGG 
ATGATTTTCT TTCGACAGGA TCAACTTCAT ATTGATTTGT TTGTCTTTTT CTCTGTGTTC 
TTCTCTTGTT TCTTTTTATT TTTAGCCGGT TGTGTAGTTG CATGGAAAAC GAAACAAGCA 
GCGGATGTTC GAAGAGCACG GAGAAGACAT GTTGTCGAAA TGTTGCATAT GGCTAAAAGA 
CCTTTCGCAT CAGCTACCAT CATTTACGAT CGAGATGGTA ATGACTGTAA TCCAAGCTCT 
CCTCAGCGAA AAAGCAGACG TAATAAATTA GTCAGCTTTC ATAGTGATGT TAGACCTGTA 
GCAGTTGAAC CTACTGATGA TGGAGTTGCG GCTGTAGCCA CTGTTTTCAT TAGATTGCCT 
GGAGGTCGAC AAGCTCCAGT TAAATTAGCT CTTGGTAGCT CATTAATACT ATTAACTAGA 
GTTTATCCAG TTAATAGTAG AGTGTTCCTA AGACGTAGAA ACAGTCACGC GTTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC725895 ( XM_026439723.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026295508.1 Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X4, mRNA.

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CloneID	OHd29707
Clone ID Related Accession (Same CDS sequence)	XM_026439720.1 , XM_026439723.1 , XM_026439722.1 , XM_026439721.1
Accession Version	XM_026439723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	multiple epidermal growth factor-like domains protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037640.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGTA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATACA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAACAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTTTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTACGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTATAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTATAT 
CATCAAGCTA CTAATTCTTT ATTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGACTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCAACTGAAA TAGTTCATAG TAAACAACAA 
ATATATATTT ATGGTGGCTT GACAATGAAA CAAGAAGATA TTCAAGGTTT ATCAAATAAC 
ACTCTAAACG ATTTTTGGAA ATTTAGTCTA CAAAATCAAA GATGGATGCA AATCATACAA 
GATAAGTTTA AAAAAGAACC ACCATCTTTA GCTGGACATA CATTAACTCT TCGTAGAAAT 
GGAGAATCAG AAAGTCTAAT ATTAATTGGA GGATTCAGTC CTAAGTATGG TTATCTTGAC 
ACAGTATGGG AATTCAATTT AGAAACAGAA GCTTGGGATA CAATAAATAC AATTGGAAAT 
GGACCATTAG GCGTATATGG CCATTCAACT GTATATCATA GCAAGTCTGA TAGTTTTTAT 
ATTTTCGGTG GATACACTTA TGCGATAAAT CGAACATTTA TTTCTAATAA ATTATACGCA 
TTGAATTATA AAACTCGTAT GTGGTCCGTA CTTCCTCCAT TTGATGATGA TCTCACTGAT 
GGAAGTAGTT TGCCACAAGC TAGATTTCTT CATTCTGCTG TTACCACTGA TGAATATATG 
GTGATATTTG GTGGCCGTCA AAATCCACAT AATACTTCAG ACTCATTGAT TGCTTATAAA 
TATTCATGTA ATTTATGGAT TCGTTTAATA ACAAAAGATA TGGAAGTTAT TGGATATCCA 
CCACCTCCAG CATATGCTCA TGCAATGACT CATGCTGATC CAGAATCTAA TGCTGTATAT 
GTAGTTGGTG GTTTTGATGG TGGAATTAAA AGTCACGTCA CTCTTATAAG TATACCAGAA 
GATTTGTGTA ATCTTTGGAC AGATAAAATA ACTTGCAGAA AATATTTTGG ATGTTCTTTT 
TGTTCTGTTA TTACAATTAG TGGAAATAAT GCTTCATTTT GTTTTTCTAA TGAAATATCA 
ATTAATAAGG ATGATAGATG TGATATTAAT GTTACTCAAG CTCAACGATC AAATGGAATC 
TTTTGTAATT TAGAATGGAT GATGAGTAGA AAATGTCAAA ATTTTAAAAC TTGTACAGAA 
TGTTTAGCAG AATGGCCATA TTATAAAAGT GAAGAACCAG TGTGCAAATG GTGTACAAAT 
TGTTCAAATG GAAAGTGTAT ACCATCTGAG AAAGATTGCG ATGAATTAAA TAAATGCAGT 
GTTAGACAAA AATCAGTAAC AGATGTAAAT CAATGTGGAG AACGTCAATG TCCAGCAAGT 
GATTGCGAGA AATGTAACAA TCTTGAAGGT TGTGTATGGA CAAGGCAGGT TTTGAAAACT 
TCTGAATTGG GACTTGCAGT AACAGGAGAA CCTGTCTATG ATTGGAATTG TGTACCAGAA 
CATATTTTTG AAAGATCAAG TATTAAAATG GGAAGTACGC AATGTGAAAA ACGATGTGGT 
GATCATAAGG ACTGTAAAAA CTGTTTAAAA GGTACAGGAG CTGAAGGTGG TTGGCGAGAA 
TGTAGATGGT CAACGCAACT TAATGAATGT ATTTCTCCAT CATATCAACC TCTGTACTGT 
GCTGGCGGTG TATGTGGTTT GGTATTACGT AGTTCTGATA TGGATCATTG TCCTGAACCA 
TGTTCAGTTT TTAAACAATG CAGTACATGT TTGAAACATT CACACTGTGG ATGGTGTTCT 
TTAGATTCAG CTAATATAAC TGGTCAAGGA ATATGTACAG AAGGTTCATT AGAAGCACCA 
GCAGATCATC CAGCTGGTGG AACATGTGAA ATGTTATATT ATCAACAGTT ACCCACAGTT 
GAACTACTAA AGTTTCTGGA AATCTCAGAC AATGTTACAA TGTTAATTCC AAACGTGATG 
TCAAAACCAA TATTTTCTTG GCATTATGTA CGATGCCCGC CAGAAAATGA ATGTATGAAT 
GGACACCACA CATGTTCCAC AAAAAGTGAA AAATGTTTTG ATTTAGAAGA AGGATTTGAA 
TGCATGTGTG GCGACGGATA TAAAACTGAA ACTCCATCAT CATTTAATGA ATTTGGAAGA 
AAAATTTGCG TTCCAATGTG TACACAAGGT TGTGTTAGAG GAACATGCAT AGAACCAAAT 
ATTTGTCGTT GTGACTTTGG TTATGTAGGT GCCAATTGTT CCATTCAGTG CCAATGTAAT 
GGTCATAGTA ATTGTGCAGG CCCAGATAAA TTAGACGTTT GTTTAGAATG TCATAATAAT 
ACAATGGGAC CACAATGTGA CAAATGTAGA CCTTTATTTG TTGGAAATCC AGCAGATAAT 
GGTCAATGTG TACCATGTCT TGAATATTGC AATGGACATA CTCGAATATG TATTAATGAA 
AGCATGACTG TACCGGATCC AAATTCAATT GAAAAAATGT CTATAGAATT ACTAGAAAAA 
CAATTAGTCG AAGGTCCTGT AGCTAAAGCT AAATGCGTAA ATTGTGGAAA TAATACAAGA 
GGTGACAAAT GTGGTGAATG TATGATTGGT TATTTCAGAG GAACAGAAGA TCTTCGTGAT 
ATATGCCGAT CTTGTCAATG TCATGGACAT GGATTTACTT GTGATCCAGT AACAGGAGAA 
AAATGCAATT GTGGTAATAA TACAGAAAGT GATCCAACTT GTACTAGTGG ACCTATGAAA 
AGTACAAATA CTGGTAGTTC CCCATGTTGG ATGGTACAAT GTAGTAAATG TAAAGAAAAT 
TATGCTGGGA CACCAACAAT GGGTCATCAA TGTTATAAAA CTGTAACTGT TGACAACAAA 
ATGTGCTTTG ACTCTAAATT AATAGGAGGC TCTTATGATG AGTGTAAAAT GAAACCAAAA 
CCATTGAATC CCGGTCAAAC GGTATTTTAT ATGGTACAGC CTCGTTTTAT GAATGTTGAT 
ATTAGAGTTA TGGTGGATGT TACTCAAGGG GCTTTAGATC TATTTCTTAG CCCTCGAGAT 
GATTCTTTCG TTGTTACATT AAATTCAACA ACAGGATATC AAGATGTTGA ACTGGACAGT 
AGATTTATGT GGCGAAAAAA TTCACATAAT ATGTGGTTAG ATGAACAAAC TCGTAGTCAA 
ATGAGATTAG TTGAATTCCA ACCTCATAAT ACTTTTTCAT TTGCTTTAAA TGGCACTACA 
ACTGAACCGA CTTGGAATAC TGGCCCTCAG TATATTGTAA TGGAAAGATA TGCAGAAGGT 
TTAGCTACAT TTTTAACAAT CGAGAATAGA AATACATTCT TGGTAGTTAG AAATCTTACA 
AATCGGTTAG TGTTGACATT ACCACAAGAC AAACATGAAC TTCATGAAAC AAAATTTCAT 
ATCGCATTAA GAGCGATTGA TCCTGTGCAT CCAGAAATTA GTGGTCGAGC TGCTTACGGG 
ATGATTTTCT TTCGACAGGA TCAACTTCAT ATTGATTTGT TTGTCTTTTT CTCTGTGTTC 
TTCTCTTGTT TCTTTTTATT TTTAGCCGGT TGTGTAGTTG CATGGAAAAC GAAACAAGCA 
GCGGATGTTC GAAGAGCACG GAGAAGACAT GTTGTCGAAA TGTTGCATAT GGCTAAAAGA 
CCTTTCGCAT CAGCTACCAT CATTTACGAT CGAGATGGTA ATGACTGTAA TCCAAGCTCT 
CCTCAGCGAA AAAGCAGACG TAATAAATTA GTCAGCTTTC ATAGTGATGT TAGACCTGTA 
GCAGTTGAAC CTACTGATGA TGGAGTTGCG GCTGTAGCCA CTGTTTTCAT TAGATTGCCT 
GGAGGTCGAC AAGCTCCAGT TAAATTAGCT CTTGGTAGCT CATTAATACT ATTAACTAGA 
GTTTATCCAG TTAATAGTAG AGTGTTCCTA AGACGTAGAA ACAGTCACGC GTTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026295508.1
CDS	1..9120
Translation	MGWVLSTIIWITIWSCLLRGFELAPQISPKQIPCDKTRKVFTDSWGIISDGPLGSNYTQDSHCEWLIKANNSRQFITLSFRTMGTECSYDYVFVYDGDSFRSPLLGSFSGKTEPQQVTSSSGYMLILLYSDTNYVLDGFHAEFSVTNCSNNCTNHGKCIDNTCFCEDDWGGRDCSRALCPNNCSYSGKCDLKRCHCHNGYSGQSCSLHKMHPESNKWHWLSHSEGGLRPRAAHTAVYIKETDSLYVFGGYDLNYILDNLEVYRFNISQWEDEYGNELEGISSAEHLDPILLALELKGTNTDEKELYGLRTSSFIWKLLSGIKDNNNTFNSRNLANVEKRYGWSEFRIGDREKKIHETKDNETSRSTVRIERNDDLKRKHSRHPRPRYAQTARHRRNLENLDFYKKFHTSNIKSDILNWEEEIAEDSIGDNIYVQEPKIRDELFKDESIKSSIDKLPKPSPRYGHAACRYDGGFVIYGGKVQDGSLSNELWYYNVNTRSWTLRAKNSPFYPPQLTRHTLTLANNYIYLFGGSTVDGEFSSNLYKIKLNLSDPTATTEKWKEVHPRGGKELDVRVVAHSTVYHQATNSLLIYGGVVASVARFSKLSDRMFVFQLDKKVWSEIHYPRAHLRDTYVPRERAFHTCNIIGNYLVVFGGYSHRHNKEEICYDNQMYLYHLGCHAWVSHDVLGLNDKDSRYPKQQGVFAHAADVRNGNTLLLVGGYHGNVNADLLAYTLPPMLAPGDEDNIEPEQICSRHKSLMECTANPECGWCSADEICYGRTIGSNCTTNLQTTRCLGVCPALGDCHSCLIHGQPGGGWGTTARGKKSVSNKLNRGTCTWCVQNARCHHKDDNYGVCGLRDDTLSQIPGWWGSKGTEIIKVEECREMDKRPGLTFLKYKPPVNFTQPDSVTIVNATTVDFSVPSMQGAKTESALGGEMIARLIGFLRPPQDFWDSTLEHLKICVSYNSATLHVSRSDDPQELELVANLTADTSQCIPTKWPDGYQMMLLPGRYLLDFESKRMVTASYAYASKMEIIHNKKTENPKVFTFEYLEPFQNGSCQQYKNCLHCLTDSSCGWCDIINKCLSRSINETESCIADVEWDEDGNPIREWHYLTITPSACANCSNYISCESCVNSNLCEWWTEEARCARIGRLPNAVVSFDQCPIPCRQRSNCTQCLDERGRCVWCEATQECFSFSVYTSEYQFGLCREWMDQAGLMGVTSRSGSSLTGNDQCKSCTRHTNCSSCLHSLSCGWCYSLENPIIGACVQGDFNQPHVNCSLIINEYQNTTLEIDESGWAYAQCPDVDECDLGLHDCHLNAVCTNTHGSFSCQCKRGFNGDGKENCTKTCYEKCVNGYCSEAPDYKCECNLGWTGPDCRTNCGCYNHSTCIQGPGICDKCQDWTTGTYCEECKAGSYGNATTPLGCKKCNCNEHGDKDLGICDRQTGICFCRDNTQGDMCQRCKKGYYGDPRDGGMCYYSCMSRGMLGGEGNGKQGLGSRHSQLSLWESHFGDSPTRECLWIVSPKTDLSSDNMLSGIQNVIQFTIHDDINVSCQENSVYVYDGLPEFVSSVTSHQSQLLGVYCTESTDYPVTVEAKSGFLTVHYKQLDEVEGFNASYLIMTCNNCPGNRECRNGNCLCKAGFVGINCDIEICPNNCTSTKKQGICDKGYGRCVCIPGYGGRDCSIKIKEHQLIFTELFNSEYLADHLDHLRKTLPRFGHTLVADRRGSLWMFGGYSLSHGPLNDIRLFDTKNNTWMPITVESTSKASMPQGRYFHATEIVHSKQQIYIYGGLTMKQEDIQGLSNNTLNDFWKFSLQNQRWMQIIQDKFKKEPPSLAGHTLTLRRNGESESLILIGGFSPKYGYLDTVWEFNLETEAWDTINTIGNGPLGVYGHSTVYHSKSDSFYIFGGYTYAINRTFISNKLYALNYKTRMWSVLPPFDDDLTDGSSLPQARFLHSAVTTDEYMVIFGGRQNPHNTSDSLIAYKYSCNLWIRLITKDMEVIGYPPPPAYAHAMTHADPESNAVYVVGGFDGGIKSHVTLISIPEDLCNLWTDKITCRKYFGCSFCSVITISGNNASFCFSNEISINKDDRCDINVTQAQRSNGIFCNLEWMMSRKCQNFKTCTECLAEWPYYKSEEPVCKWCTNCSNGKCIPSEKDCDELNKCSVRQKSVTDVNQCGERQCPASDCEKCNNLEGCVWTRQVLKTSELGLAVTGEPVYDWNCVPEHIFERSSIKMGSTQCEKRCGDHKDCKNCLKGTGAEGGWRECRWSTQLNECISPSYQPLYCAGGVCGLVLRSSDMDHCPEPCSVFKQCSTCLKHSHCGWCSLDSANITGQGICTEGSLEAPADHPAGGTCEMLYYQQLPTVELLKFLEISDNVTMLIPNVMSKPIFSWHYVRCPPENECMNGHHTCSTKSEKCFDLEEGFECMCGDGYKTETPSSFNEFGRKICVPMCTQGCVRGTCIEPNICRCDFGYVGANCSIQCQCNGHSNCAGPDKLDVCLECHNNTMGPQCDKCRPLFVGNPADNGQCVPCLEYCNGHTRICINESMTVPDPNSIEKMSIELLEKQLVEGPVAKAKCVNCGNNTRGDKCGECMIGYFRGTEDLRDICRSCQCHGHGFTCDPVTGEKCNCGNNTESDPTCTSGPMKSTNTGSSPCWMVQCSKCKENYAGTPTMGHQCYKTVTVDNKMCFDSKLIGGSYDECKMKPKPLNPGQTVFYMVQPRFMNVDIRVMVDVTQGALDLFLSPRDDSFVVTLNSTTGYQDVELDSRFMWRKNSHNMWLDEQTRSQMRLVEFQPHNTFSFALNGTTTEPTWNTGPQYIVMERYAEGLATFLTIENRNTFLVVRNLTNRLVLTLPQDKHELHETKFHIALRAIDPVHPEISGRAAYGMIFFRQDQLHIDLFVFFSVFFSCFFLFLAGCVVAWKTKQAADVRRARRRHVVEMLHMAKRPFASATIIYDRDGNDCNPSSPQRKSRRNKLVSFHSDVRPVAVEPTDDGVAAVATVFIRLPGGRQAPVKLALGSSLILLTRVYPVNSRVFLRRRNSHALN

Target ORF information:

RefSeq Version	XM_026439723.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026439723.1

ORF Insert Sequence:

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGTA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATACA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAACAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTTTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTACGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTATAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTATAT 
CATCAAGCTA CTAATTCTTT ATTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGACTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCAACTGAAA TAGTTCATAG TAAACAACAA 
ATATATATTT ATGGTGGCTT GACAATGAAA CAAGAAGATA TTCAAGGTTT ATCAAATAAC 
ACTCTAAACG ATTTTTGGAA ATTTAGTCTA CAAAATCAAA GATGGATGCA AATCATACAA 
GATAAGTTTA AAAAAGAACC ACCATCTTTA GCTGGACATA CATTAACTCT TCGTAGAAAT 
GGAGAATCAG AAAGTCTAAT ATTAATTGGA GGATTCAGTC CTAAGTATGG TTATCTTGAC 
ACAGTATGGG AATTCAATTT AGAAACAGAA GCTTGGGATA CAATAAATAC AATTGGAAAT 
GGACCATTAG GCGTATATGG CCATTCAACT GTATATCATA GCAAGTCTGA TAGTTTTTAT 
ATTTTCGGTG GATACACTTA TGCGATAAAT CGAACATTTA TTTCTAATAA ATTATACGCA 
TTGAATTATA AAACTCGTAT GTGGTCCGTA CTTCCTCCAT TTGATGATGA TCTCACTGAT 
GGAAGTAGTT TGCCACAAGC TAGATTTCTT CATTCTGCTG TTACCACTGA TGAATATATG 
GTGATATTTG GTGGCCGTCA AAATCCACAT AATACTTCAG ACTCATTGAT TGCTTATAAA 
TATTCATGTA ATTTATGGAT TCGTTTAATA ACAAAAGATA TGGAAGTTAT TGGATATCCA 
CCACCTCCAG CATATGCTCA TGCAATGACT CATGCTGATC CAGAATCTAA TGCTGTATAT 
GTAGTTGGTG GTTTTGATGG TGGAATTAAA AGTCACGTCA CTCTTATAAG TATACCAGAA 
GATTTGTGTA ATCTTTGGAC AGATAAAATA ACTTGCAGAA AATATTTTGG ATGTTCTTTT 
TGTTCTGTTA TTACAATTAG TGGAAATAAT GCTTCATTTT GTTTTTCTAA TGAAATATCA 
ATTAATAAGG ATGATAGATG TGATATTAAT GTTACTCAAG CTCAACGATC AAATGGAATC 
TTTTGTAATT TAGAATGGAT GATGAGTAGA AAATGTCAAA ATTTTAAAAC TTGTACAGAA 
TGTTTAGCAG AATGGCCATA TTATAAAAGT GAAGAACCAG TGTGCAAATG GTGTACAAAT 
TGTTCAAATG GAAAGTGTAT ACCATCTGAG AAAGATTGCG ATGAATTAAA TAAATGCAGT 
GTTAGACAAA AATCAGTAAC AGATGTAAAT CAATGTGGAG AACGTCAATG TCCAGCAAGT 
GATTGCGAGA AATGTAACAA TCTTGAAGGT TGTGTATGGA CAAGGCAGGT TTTGAAAACT 
TCTGAATTGG GACTTGCAGT AACAGGAGAA CCTGTCTATG ATTGGAATTG TGTACCAGAA 
CATATTTTTG AAAGATCAAG TATTAAAATG GGAAGTACGC AATGTGAAAA ACGATGTGGT 
GATCATAAGG ACTGTAAAAA CTGTTTAAAA GGTACAGGAG CTGAAGGTGG TTGGCGAGAA 
TGTAGATGGT CAACGCAACT TAATGAATGT ATTTCTCCAT CATATCAACC TCTGTACTGT 
GCTGGCGGTG TATGTGGTTT GGTATTACGT AGTTCTGATA TGGATCATTG TCCTGAACCA 
TGTTCAGTTT TTAAACAATG CAGTACATGT TTGAAACATT CACACTGTGG ATGGTGTTCT 
TTAGATTCAG CTAATATAAC TGGTCAAGGA ATATGTACAG AAGGTTCATT AGAAGCACCA 
GCAGATCATC CAGCTGGTGG AACATGTGAA ATGTTATATT ATCAACAGTT ACCCACAGTT 
GAACTACTAA AGTTTCTGGA AATCTCAGAC AATGTTACAA TGTTAATTCC AAACGTGATG 
TCAAAACCAA TATTTTCTTG GCATTATGTA CGATGCCCGC CAGAAAATGA ATGTATGAAT 
GGACACCACA CATGTTCCAC AAAAAGTGAA AAATGTTTTG ATTTAGAAGA AGGATTTGAA 
TGCATGTGTG GCGACGGATA TAAAACTGAA ACTCCATCAT CATTTAATGA ATTTGGAAGA 
AAAATTTGCG TTCCAATGTG TACACAAGGT TGTGTTAGAG GAACATGCAT AGAACCAAAT 
ATTTGTCGTT GTGACTTTGG TTATGTAGGT GCCAATTGTT CCATTCAGTG CCAATGTAAT 
GGTCATAGTA ATTGTGCAGG CCCAGATAAA TTAGACGTTT GTTTAGAATG TCATAATAAT 
ACAATGGGAC CACAATGTGA CAAATGTAGA CCTTTATTTG TTGGAAATCC AGCAGATAAT 
GGTCAATGTG TACCATGTCT TGAATATTGC AATGGACATA CTCGAATATG TATTAATGAA 
AGCATGACTG TACCGGATCC AAATTCAATT GAAAAAATGT CTATAGAATT ACTAGAAAAA 
CAATTAGTCG AAGGTCCTGT AGCTAAAGCT AAATGCGTAA ATTGTGGAAA TAATACAAGA 
GGTGACAAAT GTGGTGAATG TATGATTGGT TATTTCAGAG GAACAGAAGA TCTTCGTGAT 
ATATGCCGAT CTTGTCAATG TCATGGACAT GGATTTACTT GTGATCCAGT AACAGGAGAA 
AAATGCAATT GTGGTAATAA TACAGAAAGT GATCCAACTT GTACTAGTGG ACCTATGAAA 
AGTACAAATA CTGGTAGTTC CCCATGTTGG ATGGTACAAT GTAGTAAATG TAAAGAAAAT 
TATGCTGGGA CACCAACAAT GGGTCATCAA TGTTATAAAA CTGTAACTGT TGACAACAAA 
ATGTGCTTTG ACTCTAAATT AATAGGAGGC TCTTATGATG AGTGTAAAAT GAAACCAAAA 
CCATTGAATC CCGGTCAAAC GGTATTTTAT ATGGTACAGC CTCGTTTTAT GAATGTTGAT 
ATTAGAGTTA TGGTGGATGT TACTCAAGGG GCTTTAGATC TATTTCTTAG CCCTCGAGAT 
GATTCTTTCG TTGTTACATT AAATTCAACA ACAGGATATC AAGATGTTGA ACTGGACAGT 
AGATTTATGT GGCGAAAAAA TTCACATAAT ATGTGGTTAG ATGAACAAAC TCGTAGTCAA 
ATGAGATTAG TTGAATTCCA ACCTCATAAT ACTTTTTCAT TTGCTTTAAA TGGCACTACA 
ACTGAACCGA CTTGGAATAC TGGCCCTCAG TATATTGTAA TGGAAAGATA TGCAGAAGGT 
TTAGCTACAT TTTTAACAAT CGAGAATAGA AATACATTCT TGGTAGTTAG AAATCTTACA 
AATCGGTTAG TGTTGACATT ACCACAAGAC AAACATGAAC TTCATGAAAC AAAATTTCAT 
ATCGCATTAA GAGCGATTGA TCCTGTGCAT CCAGAAATTA GTGGTCGAGC TGCTTACGGG 
ATGATTTTCT TTCGACAGGA TCAACTTCAT ATTGATTTGT TTGTCTTTTT CTCTGTGTTC 
TTCTCTTGTT TCTTTTTATT TTTAGCCGGT TGTGTAGTTG CATGGAAAAC GAAACAAGCA 
GCGGATGTTC GAAGAGCACG GAGAAGACAT GTTGTCGAAA TGTTGCATAT GGCTAAAAGA 
CCTTTCGCAT CAGCTACCAT CATTTACGAT CGAGATGGTA ATGACTGTAA TCCAAGCTCT 
CCTCAGCGAA AAAGCAGACG TAATAAATTA GTCAGCTTTC ATAGTGATGT TAGACCTGTA 
GCAGTTGAAC CTACTGATGA TGGAGTTGCG GCTGTAGCCA CTGTTTTCAT TAGATTGCCT 
GGAGGTCGAC AAGCTCCAGT TAAATTAGCT CTTGGTAGCT CATTAATACT ATTAACTAGA 
GTTTATCCAG TTAATAGTAG AGTGTTCCTA AGACGTAGAA ACAGTCACGC GTTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC725895 ( XM_026439722.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026295507.1 Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X3, mRNA.

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CloneID	OHd29707
Clone ID Related Accession (Same CDS sequence)	XM_026439720.1 , XM_026439723.1 , XM_026439722.1 , XM_026439721.1
Accession Version	XM_026439722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	multiple epidermal growth factor-like domains protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037640.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGTA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATACA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAACAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTTTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTACGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTATAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTATAT 
CATCAAGCTA CTAATTCTTT ATTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGACTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCAACTGAAA TAGTTCATAG TAAACAACAA 
ATATATATTT ATGGTGGCTT GACAATGAAA CAAGAAGATA TTCAAGGTTT ATCAAATAAC 
ACTCTAAACG ATTTTTGGAA ATTTAGTCTA CAAAATCAAA GATGGATGCA AATCATACAA 
GATAAGTTTA AAAAAGAACC ACCATCTTTA GCTGGACATA CATTAACTCT TCGTAGAAAT 
GGAGAATCAG AAAGTCTAAT ATTAATTGGA GGATTCAGTC CTAAGTATGG TTATCTTGAC 
ACAGTATGGG AATTCAATTT AGAAACAGAA GCTTGGGATA CAATAAATAC AATTGGAAAT 
GGACCATTAG GCGTATATGG CCATTCAACT GTATATCATA GCAAGTCTGA TAGTTTTTAT 
ATTTTCGGTG GATACACTTA TGCGATAAAT CGAACATTTA TTTCTAATAA ATTATACGCA 
TTGAATTATA AAACTCGTAT GTGGTCCGTA CTTCCTCCAT TTGATGATGA TCTCACTGAT 
GGAAGTAGTT TGCCACAAGC TAGATTTCTT CATTCTGCTG TTACCACTGA TGAATATATG 
GTGATATTTG GTGGCCGTCA AAATCCACAT AATACTTCAG ACTCATTGAT TGCTTATAAA 
TATTCATGTA ATTTATGGAT TCGTTTAATA ACAAAAGATA TGGAAGTTAT TGGATATCCA 
CCACCTCCAG CATATGCTCA TGCAATGACT CATGCTGATC CAGAATCTAA TGCTGTATAT 
GTAGTTGGTG GTTTTGATGG TGGAATTAAA AGTCACGTCA CTCTTATAAG TATACCAGAA 
GATTTGTGTA ATCTTTGGAC AGATAAAATA ACTTGCAGAA AATATTTTGG ATGTTCTTTT 
TGTTCTGTTA TTACAATTAG TGGAAATAAT GCTTCATTTT GTTTTTCTAA TGAAATATCA 
ATTAATAAGG ATGATAGATG TGATATTAAT GTTACTCAAG CTCAACGATC AAATGGAATC 
TTTTGTAATT TAGAATGGAT GATGAGTAGA AAATGTCAAA ATTTTAAAAC TTGTACAGAA 
TGTTTAGCAG AATGGCCATA TTATAAAAGT GAAGAACCAG TGTGCAAATG GTGTACAAAT 
TGTTCAAATG GAAAGTGTAT ACCATCTGAG AAAGATTGCG ATGAATTAAA TAAATGCAGT 
GTTAGACAAA AATCAGTAAC AGATGTAAAT CAATGTGGAG AACGTCAATG TCCAGCAAGT 
GATTGCGAGA AATGTAACAA TCTTGAAGGT TGTGTATGGA CAAGGCAGGT TTTGAAAACT 
TCTGAATTGG GACTTGCAGT AACAGGAGAA CCTGTCTATG ATTGGAATTG TGTACCAGAA 
CATATTTTTG AAAGATCAAG TATTAAAATG GGAAGTACGC AATGTGAAAA ACGATGTGGT 
GATCATAAGG ACTGTAAAAA CTGTTTAAAA GGTACAGGAG CTGAAGGTGG TTGGCGAGAA 
TGTAGATGGT CAACGCAACT TAATGAATGT ATTTCTCCAT CATATCAACC TCTGTACTGT 
GCTGGCGGTG TATGTGGTTT GGTATTACGT AGTTCTGATA TGGATCATTG TCCTGAACCA 
TGTTCAGTTT TTAAACAATG CAGTACATGT TTGAAACATT CACACTGTGG ATGGTGTTCT 
TTAGATTCAG CTAATATAAC TGGTCAAGGA ATATGTACAG AAGGTTCATT AGAAGCACCA 
GCAGATCATC CAGCTGGTGG AACATGTGAA ATGTTATATT ATCAACAGTT ACCCACAGTT 
GAACTACTAA AGTTTCTGGA AATCTCAGAC AATGTTACAA TGTTAATTCC AAACGTGATG 
TCAAAACCAA TATTTTCTTG GCATTATGTA CGATGCCCGC CAGAAAATGA ATGTATGAAT 
GGACACCACA CATGTTCCAC AAAAAGTGAA AAATGTTTTG ATTTAGAAGA AGGATTTGAA 
TGCATGTGTG GCGACGGATA TAAAACTGAA ACTCCATCAT CATTTAATGA ATTTGGAAGA 
AAAATTTGCG TTCCAATGTG TACACAAGGT TGTGTTAGAG GAACATGCAT AGAACCAAAT 
ATTTGTCGTT GTGACTTTGG TTATGTAGGT GCCAATTGTT CCATTCAGTG CCAATGTAAT 
GGTCATAGTA ATTGTGCAGG CCCAGATAAA TTAGACGTTT GTTTAGAATG TCATAATAAT 
ACAATGGGAC CACAATGTGA CAAATGTAGA CCTTTATTTG TTGGAAATCC AGCAGATAAT 
GGTCAATGTG TACCATGTCT TGAATATTGC AATGGACATA CTCGAATATG TATTAATGAA 
AGCATGACTG TACCGGATCC AAATTCAATT GAAAAAATGT CTATAGAATT ACTAGAAAAA 
CAATTAGTCG AAGGTCCTGT AGCTAAAGCT AAATGCGTAA ATTGTGGAAA TAATACAAGA 
GGTGACAAAT GTGGTGAATG TATGATTGGT TATTTCAGAG GAACAGAAGA TCTTCGTGAT 
ATATGCCGAT CTTGTCAATG TCATGGACAT GGATTTACTT GTGATCCAGT AACAGGAGAA 
AAATGCAATT GTGGTAATAA TACAGAAAGT GATCCAACTT GTACTAGTGG ACCTATGAAA 
AGTACAAATA CTGGTAGTTC CCCATGTTGG ATGGTACAAT GTAGTAAATG TAAAGAAAAT 
TATGCTGGGA CACCAACAAT GGGTCATCAA TGTTATAAAA CTGTAACTGT TGACAACAAA 
ATGTGCTTTG ACTCTAAATT AATAGGAGGC TCTTATGATG AGTGTAAAAT GAAACCAAAA 
CCATTGAATC CCGGTCAAAC GGTATTTTAT ATGGTACAGC CTCGTTTTAT GAATGTTGAT 
ATTAGAGTTA TGGTGGATGT TACTCAAGGG GCTTTAGATC TATTTCTTAG CCCTCGAGAT 
GATTCTTTCG TTGTTACATT AAATTCAACA ACAGGATATC AAGATGTTGA ACTGGACAGT 
AGATTTATGT GGCGAAAAAA TTCACATAAT ATGTGGTTAG ATGAACAAAC TCGTAGTCAA 
ATGAGATTAG TTGAATTCCA ACCTCATAAT ACTTTTTCAT TTGCTTTAAA TGGCACTACA 
ACTGAACCGA CTTGGAATAC TGGCCCTCAG TATATTGTAA TGGAAAGATA TGCAGAAGGT 
TTAGCTACAT TTTTAACAAT CGAGAATAGA AATACATTCT TGGTAGTTAG AAATCTTACA 
AATCGGTTAG TGTTGACATT ACCACAAGAC AAACATGAAC TTCATGAAAC AAAATTTCAT 
ATCGCATTAA GAGCGATTGA TCCTGTGCAT CCAGAAATTA GTGGTCGAGC TGCTTACGGG 
ATGATTTTCT TTCGACAGGA TCAACTTCAT ATTGATTTGT TTGTCTTTTT CTCTGTGTTC 
TTCTCTTGTT TCTTTTTATT TTTAGCCGGT TGTGTAGTTG CATGGAAAAC GAAACAAGCA 
GCGGATGTTC GAAGAGCACG GAGAAGACAT GTTGTCGAAA TGTTGCATAT GGCTAAAAGA 
CCTTTCGCAT CAGCTACCAT CATTTACGAT CGAGATGGTA ATGACTGTAA TCCAAGCTCT 
CCTCAGCGAA AAAGCAGACG TAATAAATTA GTCAGCTTTC ATAGTGATGT TAGACCTGTA 
GCAGTTGAAC CTACTGATGA TGGAGTTGCG GCTGTAGCCA CTGTTTTCAT TAGATTGCCT 
GGAGGTCGAC AAGCTCCAGT TAAATTAGCT CTTGGTAGCT CATTAATACT ATTAACTAGA 
GTTTATCCAG TTAATAGTAG AGTGTTCCTA AGACGTAGAA ACAGTCACGC GTTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026295507.1
CDS	380..9499
Translation	MGWVLSTIIWITIWSCLLRGFELAPQISPKQIPCDKTRKVFTDSWGIISDGPLGSNYTQDSHCEWLIKANNSRQFITLSFRTMGTECSYDYVFVYDGDSFRSPLLGSFSGKTEPQQVTSSSGYMLILLYSDTNYVLDGFHAEFSVTNCSNNCTNHGKCIDNTCFCEDDWGGRDCSRALCPNNCSYSGKCDLKRCHCHNGYSGQSCSLHKMHPESNKWHWLSHSEGGLRPRAAHTAVYIKETDSLYVFGGYDLNYILDNLEVYRFNISQWEDEYGNELEGISSAEHLDPILLALELKGTNTDEKELYGLRTSSFIWKLLSGIKDNNNTFNSRNLANVEKRYGWSEFRIGDREKKIHETKDNETSRSTVRIERNDDLKRKHSRHPRPRYAQTARHRRNLENLDFYKKFHTSNIKSDILNWEEEIAEDSIGDNIYVQEPKIRDELFKDESIKSSIDKLPKPSPRYGHAACRYDGGFVIYGGKVQDGSLSNELWYYNVNTRSWTLRAKNSPFYPPQLTRHTLTLANNYIYLFGGSTVDGEFSSNLYKIKLNLSDPTATTEKWKEVHPRGGKELDVRVVAHSTVYHQATNSLLIYGGVVASVARFSKLSDRMFVFQLDKKVWSEIHYPRAHLRDTYVPRERAFHTCNIIGNYLVVFGGYSHRHNKEEICYDNQMYLYHLGCHAWVSHDVLGLNDKDSRYPKQQGVFAHAADVRNGNTLLLVGGYHGNVNADLLAYTLPPMLAPGDEDNIEPEQICSRHKSLMECTANPECGWCSADEICYGRTIGSNCTTNLQTTRCLGVCPALGDCHSCLIHGQPGGGWGTTARGKKSVSNKLNRGTCTWCVQNARCHHKDDNYGVCGLRDDTLSQIPGWWGSKGTEIIKVEECREMDKRPGLTFLKYKPPVNFTQPDSVTIVNATTVDFSVPSMQGAKTESALGGEMIARLIGFLRPPQDFWDSTLEHLKICVSYNSATLHVSRSDDPQELELVANLTADTSQCIPTKWPDGYQMMLLPGRYLLDFESKRMVTASYAYASKMEIIHNKKTENPKVFTFEYLEPFQNGSCQQYKNCLHCLTDSSCGWCDIINKCLSRSINETESCIADVEWDEDGNPIREWHYLTITPSACANCSNYISCESCVNSNLCEWWTEEARCARIGRLPNAVVSFDQCPIPCRQRSNCTQCLDERGRCVWCEATQECFSFSVYTSEYQFGLCREWMDQAGLMGVTSRSGSSLTGNDQCKSCTRHTNCSSCLHSLSCGWCYSLENPIIGACVQGDFNQPHVNCSLIINEYQNTTLEIDESGWAYAQCPDVDECDLGLHDCHLNAVCTNTHGSFSCQCKRGFNGDGKENCTKTCYEKCVNGYCSEAPDYKCECNLGWTGPDCRTNCGCYNHSTCIQGPGICDKCQDWTTGTYCEECKAGSYGNATTPLGCKKCNCNEHGDKDLGICDRQTGICFCRDNTQGDMCQRCKKGYYGDPRDGGMCYYSCMSRGMLGGEGNGKQGLGSRHSQLSLWESHFGDSPTRECLWIVSPKTDLSSDNMLSGIQNVIQFTIHDDINVSCQENSVYVYDGLPEFVSSVTSHQSQLLGVYCTESTDYPVTVEAKSGFLTVHYKQLDEVEGFNASYLIMTCNNCPGNRECRNGNCLCKAGFVGINCDIEICPNNCTSTKKQGICDKGYGRCVCIPGYGGRDCSIKIKEHQLIFTELFNSEYLADHLDHLRKTLPRFGHTLVADRRGSLWMFGGYSLSHGPLNDIRLFDTKNNTWMPITVESTSKASMPQGRYFHATEIVHSKQQIYIYGGLTMKQEDIQGLSNNTLNDFWKFSLQNQRWMQIIQDKFKKEPPSLAGHTLTLRRNGESESLILIGGFSPKYGYLDTVWEFNLETEAWDTINTIGNGPLGVYGHSTVYHSKSDSFYIFGGYTYAINRTFISNKLYALNYKTRMWSVLPPFDDDLTDGSSLPQARFLHSAVTTDEYMVIFGGRQNPHNTSDSLIAYKYSCNLWIRLITKDMEVIGYPPPPAYAHAMTHADPESNAVYVVGGFDGGIKSHVTLISIPEDLCNLWTDKITCRKYFGCSFCSVITISGNNASFCFSNEISINKDDRCDINVTQAQRSNGIFCNLEWMMSRKCQNFKTCTECLAEWPYYKSEEPVCKWCTNCSNGKCIPSEKDCDELNKCSVRQKSVTDVNQCGERQCPASDCEKCNNLEGCVWTRQVLKTSELGLAVTGEPVYDWNCVPEHIFERSSIKMGSTQCEKRCGDHKDCKNCLKGTGAEGGWRECRWSTQLNECISPSYQPLYCAGGVCGLVLRSSDMDHCPEPCSVFKQCSTCLKHSHCGWCSLDSANITGQGICTEGSLEAPADHPAGGTCEMLYYQQLPTVELLKFLEISDNVTMLIPNVMSKPIFSWHYVRCPPENECMNGHHTCSTKSEKCFDLEEGFECMCGDGYKTETPSSFNEFGRKICVPMCTQGCVRGTCIEPNICRCDFGYVGANCSIQCQCNGHSNCAGPDKLDVCLECHNNTMGPQCDKCRPLFVGNPADNGQCVPCLEYCNGHTRICINESMTVPDPNSIEKMSIELLEKQLVEGPVAKAKCVNCGNNTRGDKCGECMIGYFRGTEDLRDICRSCQCHGHGFTCDPVTGEKCNCGNNTESDPTCTSGPMKSTNTGSSPCWMVQCSKCKENYAGTPTMGHQCYKTVTVDNKMCFDSKLIGGSYDECKMKPKPLNPGQTVFYMVQPRFMNVDIRVMVDVTQGALDLFLSPRDDSFVVTLNSTTGYQDVELDSRFMWRKNSHNMWLDEQTRSQMRLVEFQPHNTFSFALNGTTTEPTWNTGPQYIVMERYAEGLATFLTIENRNTFLVVRNLTNRLVLTLPQDKHELHETKFHIALRAIDPVHPEISGRAAYGMIFFRQDQLHIDLFVFFSVFFSCFFLFLAGCVVAWKTKQAADVRRARRRHVVEMLHMAKRPFASATIIYDRDGNDCNPSSPQRKSRRNKLVSFHSDVRPVAVEPTDDGVAAVATVFIRLPGGRQAPVKLALGSSLILLTRVYPVNSRVFLRRRNSHALN

Target ORF information:

RefSeq Version	XM_026439722.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026439722.1

ORF Insert Sequence:

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGTA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATACA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAACAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTTTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTACGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTATAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTATAT 
CATCAAGCTA CTAATTCTTT ATTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGACTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCAACTGAAA TAGTTCATAG TAAACAACAA 
ATATATATTT ATGGTGGCTT GACAATGAAA CAAGAAGATA TTCAAGGTTT ATCAAATAAC 
ACTCTAAACG ATTTTTGGAA ATTTAGTCTA CAAAATCAAA GATGGATGCA AATCATACAA 
GATAAGTTTA AAAAAGAACC ACCATCTTTA GCTGGACATA CATTAACTCT TCGTAGAAAT 
GGAGAATCAG AAAGTCTAAT ATTAATTGGA GGATTCAGTC CTAAGTATGG TTATCTTGAC 
ACAGTATGGG AATTCAATTT AGAAACAGAA GCTTGGGATA CAATAAATAC AATTGGAAAT 
GGACCATTAG GCGTATATGG CCATTCAACT GTATATCATA GCAAGTCTGA TAGTTTTTAT 
ATTTTCGGTG GATACACTTA TGCGATAAAT CGAACATTTA TTTCTAATAA ATTATACGCA 
TTGAATTATA AAACTCGTAT GTGGTCCGTA CTTCCTCCAT TTGATGATGA TCTCACTGAT 
GGAAGTAGTT TGCCACAAGC TAGATTTCTT CATTCTGCTG TTACCACTGA TGAATATATG 
GTGATATTTG GTGGCCGTCA AAATCCACAT AATACTTCAG ACTCATTGAT TGCTTATAAA 
TATTCATGTA ATTTATGGAT TCGTTTAATA ACAAAAGATA TGGAAGTTAT TGGATATCCA 
CCACCTCCAG CATATGCTCA TGCAATGACT CATGCTGATC CAGAATCTAA TGCTGTATAT 
GTAGTTGGTG GTTTTGATGG TGGAATTAAA AGTCACGTCA CTCTTATAAG TATACCAGAA 
GATTTGTGTA ATCTTTGGAC AGATAAAATA ACTTGCAGAA AATATTTTGG ATGTTCTTTT 
TGTTCTGTTA TTACAATTAG TGGAAATAAT GCTTCATTTT GTTTTTCTAA TGAAATATCA 
ATTAATAAGG ATGATAGATG TGATATTAAT GTTACTCAAG CTCAACGATC AAATGGAATC 
TTTTGTAATT TAGAATGGAT GATGAGTAGA AAATGTCAAA ATTTTAAAAC TTGTACAGAA 
TGTTTAGCAG AATGGCCATA TTATAAAAGT GAAGAACCAG TGTGCAAATG GTGTACAAAT 
TGTTCAAATG GAAAGTGTAT ACCATCTGAG AAAGATTGCG ATGAATTAAA TAAATGCAGT 
GTTAGACAAA AATCAGTAAC AGATGTAAAT CAATGTGGAG AACGTCAATG TCCAGCAAGT 
GATTGCGAGA AATGTAACAA TCTTGAAGGT TGTGTATGGA CAAGGCAGGT TTTGAAAACT 
TCTGAATTGG GACTTGCAGT AACAGGAGAA CCTGTCTATG ATTGGAATTG TGTACCAGAA 
CATATTTTTG AAAGATCAAG TATTAAAATG GGAAGTACGC AATGTGAAAA ACGATGTGGT 
GATCATAAGG ACTGTAAAAA CTGTTTAAAA GGTACAGGAG CTGAAGGTGG TTGGCGAGAA 
TGTAGATGGT CAACGCAACT TAATGAATGT ATTTCTCCAT CATATCAACC TCTGTACTGT 
GCTGGCGGTG TATGTGGTTT GGTATTACGT AGTTCTGATA TGGATCATTG TCCTGAACCA 
TGTTCAGTTT TTAAACAATG CAGTACATGT TTGAAACATT CACACTGTGG ATGGTGTTCT 
TTAGATTCAG CTAATATAAC TGGTCAAGGA ATATGTACAG AAGGTTCATT AGAAGCACCA 
GCAGATCATC CAGCTGGTGG AACATGTGAA ATGTTATATT ATCAACAGTT ACCCACAGTT 
GAACTACTAA AGTTTCTGGA AATCTCAGAC AATGTTACAA TGTTAATTCC AAACGTGATG 
TCAAAACCAA TATTTTCTTG GCATTATGTA CGATGCCCGC CAGAAAATGA ATGTATGAAT 
GGACACCACA CATGTTCCAC AAAAAGTGAA AAATGTTTTG ATTTAGAAGA AGGATTTGAA 
TGCATGTGTG GCGACGGATA TAAAACTGAA ACTCCATCAT CATTTAATGA ATTTGGAAGA 
AAAATTTGCG TTCCAATGTG TACACAAGGT TGTGTTAGAG GAACATGCAT AGAACCAAAT 
ATTTGTCGTT GTGACTTTGG TTATGTAGGT GCCAATTGTT CCATTCAGTG CCAATGTAAT 
GGTCATAGTA ATTGTGCAGG CCCAGATAAA TTAGACGTTT GTTTAGAATG TCATAATAAT 
ACAATGGGAC CACAATGTGA CAAATGTAGA CCTTTATTTG TTGGAAATCC AGCAGATAAT 
GGTCAATGTG TACCATGTCT TGAATATTGC AATGGACATA CTCGAATATG TATTAATGAA 
AGCATGACTG TACCGGATCC AAATTCAATT GAAAAAATGT CTATAGAATT ACTAGAAAAA 
CAATTAGTCG AAGGTCCTGT AGCTAAAGCT AAATGCGTAA ATTGTGGAAA TAATACAAGA 
GGTGACAAAT GTGGTGAATG TATGATTGGT TATTTCAGAG GAACAGAAGA TCTTCGTGAT 
ATATGCCGAT CTTGTCAATG TCATGGACAT GGATTTACTT GTGATCCAGT AACAGGAGAA 
AAATGCAATT GTGGTAATAA TACAGAAAGT GATCCAACTT GTACTAGTGG ACCTATGAAA 
AGTACAAATA CTGGTAGTTC CCCATGTTGG ATGGTACAAT GTAGTAAATG TAAAGAAAAT 
TATGCTGGGA CACCAACAAT GGGTCATCAA TGTTATAAAA CTGTAACTGT TGACAACAAA 
ATGTGCTTTG ACTCTAAATT AATAGGAGGC TCTTATGATG AGTGTAAAAT GAAACCAAAA 
CCATTGAATC CCGGTCAAAC GGTATTTTAT ATGGTACAGC CTCGTTTTAT GAATGTTGAT 
ATTAGAGTTA TGGTGGATGT TACTCAAGGG GCTTTAGATC TATTTCTTAG CCCTCGAGAT 
GATTCTTTCG TTGTTACATT AAATTCAACA ACAGGATATC AAGATGTTGA ACTGGACAGT 
AGATTTATGT GGCGAAAAAA TTCACATAAT ATGTGGTTAG ATGAACAAAC TCGTAGTCAA 
ATGAGATTAG TTGAATTCCA ACCTCATAAT ACTTTTTCAT TTGCTTTAAA TGGCACTACA 
ACTGAACCGA CTTGGAATAC TGGCCCTCAG TATATTGTAA TGGAAAGATA TGCAGAAGGT 
TTAGCTACAT TTTTAACAAT CGAGAATAGA AATACATTCT TGGTAGTTAG AAATCTTACA 
AATCGGTTAG TGTTGACATT ACCACAAGAC AAACATGAAC TTCATGAAAC AAAATTTCAT 
ATCGCATTAA GAGCGATTGA TCCTGTGCAT CCAGAAATTA GTGGTCGAGC TGCTTACGGG 
ATGATTTTCT TTCGACAGGA TCAACTTCAT ATTGATTTGT TTGTCTTTTT CTCTGTGTTC 
TTCTCTTGTT TCTTTTTATT TTTAGCCGGT TGTGTAGTTG CATGGAAAAC GAAACAAGCA 
GCGGATGTTC GAAGAGCACG GAGAAGACAT GTTGTCGAAA TGTTGCATAT GGCTAAAAGA 
CCTTTCGCAT CAGCTACCAT CATTTACGAT CGAGATGGTA ATGACTGTAA TCCAAGCTCT 
CCTCAGCGAA AAAGCAGACG TAATAAATTA GTCAGCTTTC ATAGTGATGT TAGACCTGTA 
GCAGTTGAAC CTACTGATGA TGGAGTTGCG GCTGTAGCCA CTGTTTTCAT TAGATTGCCT 
GGAGGTCGAC AAGCTCCAGT TAAATTAGCT CTTGGTAGCT CATTAATACT ATTAACTAGA 
GTTTATCCAG TTAATAGTAG AGTGTTCCTA AGACGTAGAA ACAGTCACGC GTTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC725895 ( XM_026439721.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026295506.1 Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X2, mRNA.

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CloneID	OHd29707
Clone ID Related Accession (Same CDS sequence)	XM_026439720.1 , XM_026439723.1 , XM_026439722.1 , XM_026439721.1
Accession Version	XM_026439721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	multiple epidermal growth factor-like domains protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037640.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGTA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATACA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAACAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTTTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTACGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTATAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTATAT 
CATCAAGCTA CTAATTCTTT ATTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGACTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCAACTGAAA TAGTTCATAG TAAACAACAA 
ATATATATTT ATGGTGGCTT GACAATGAAA CAAGAAGATA TTCAAGGTTT ATCAAATAAC 
ACTCTAAACG ATTTTTGGAA ATTTAGTCTA CAAAATCAAA GATGGATGCA AATCATACAA 
GATAAGTTTA AAAAAGAACC ACCATCTTTA GCTGGACATA CATTAACTCT TCGTAGAAAT 
GGAGAATCAG AAAGTCTAAT ATTAATTGGA GGATTCAGTC CTAAGTATGG TTATCTTGAC 
ACAGTATGGG AATTCAATTT AGAAACAGAA GCTTGGGATA CAATAAATAC AATTGGAAAT 
GGACCATTAG GCGTATATGG CCATTCAACT GTATATCATA GCAAGTCTGA TAGTTTTTAT 
ATTTTCGGTG GATACACTTA TGCGATAAAT CGAACATTTA TTTCTAATAA ATTATACGCA 
TTGAATTATA AAACTCGTAT GTGGTCCGTA CTTCCTCCAT TTGATGATGA TCTCACTGAT 
GGAAGTAGTT TGCCACAAGC TAGATTTCTT CATTCTGCTG TTACCACTGA TGAATATATG 
GTGATATTTG GTGGCCGTCA AAATCCACAT AATACTTCAG ACTCATTGAT TGCTTATAAA 
TATTCATGTA ATTTATGGAT TCGTTTAATA ACAAAAGATA TGGAAGTTAT TGGATATCCA 
CCACCTCCAG CATATGCTCA TGCAATGACT CATGCTGATC CAGAATCTAA TGCTGTATAT 
GTAGTTGGTG GTTTTGATGG TGGAATTAAA AGTCACGTCA CTCTTATAAG TATACCAGAA 
GATTTGTGTA ATCTTTGGAC AGATAAAATA ACTTGCAGAA AATATTTTGG ATGTTCTTTT 
TGTTCTGTTA TTACAATTAG TGGAAATAAT GCTTCATTTT GTTTTTCTAA TGAAATATCA 
ATTAATAAGG ATGATAGATG TGATATTAAT GTTACTCAAG CTCAACGATC AAATGGAATC 
TTTTGTAATT TAGAATGGAT GATGAGTAGA AAATGTCAAA ATTTTAAAAC TTGTACAGAA 
TGTTTAGCAG AATGGCCATA TTATAAAAGT GAAGAACCAG TGTGCAAATG GTGTACAAAT 
TGTTCAAATG GAAAGTGTAT ACCATCTGAG AAAGATTGCG ATGAATTAAA TAAATGCAGT 
GTTAGACAAA AATCAGTAAC AGATGTAAAT CAATGTGGAG AACGTCAATG TCCAGCAAGT 
GATTGCGAGA AATGTAACAA TCTTGAAGGT TGTGTATGGA CAAGGCAGGT TTTGAAAACT 
TCTGAATTGG GACTTGCAGT AACAGGAGAA CCTGTCTATG ATTGGAATTG TGTACCAGAA 
CATATTTTTG AAAGATCAAG TATTAAAATG GGAAGTACGC AATGTGAAAA ACGATGTGGT 
GATCATAAGG ACTGTAAAAA CTGTTTAAAA GGTACAGGAG CTGAAGGTGG TTGGCGAGAA 
TGTAGATGGT CAACGCAACT TAATGAATGT ATTTCTCCAT CATATCAACC TCTGTACTGT 
GCTGGCGGTG TATGTGGTTT GGTATTACGT AGTTCTGATA TGGATCATTG TCCTGAACCA 
TGTTCAGTTT TTAAACAATG CAGTACATGT TTGAAACATT CACACTGTGG ATGGTGTTCT 
TTAGATTCAG CTAATATAAC TGGTCAAGGA ATATGTACAG AAGGTTCATT AGAAGCACCA 
GCAGATCATC CAGCTGGTGG AACATGTGAA ATGTTATATT ATCAACAGTT ACCCACAGTT 
GAACTACTAA AGTTTCTGGA AATCTCAGAC AATGTTACAA TGTTAATTCC AAACGTGATG 
TCAAAACCAA TATTTTCTTG GCATTATGTA CGATGCCCGC CAGAAAATGA ATGTATGAAT 
GGACACCACA CATGTTCCAC AAAAAGTGAA AAATGTTTTG ATTTAGAAGA AGGATTTGAA 
TGCATGTGTG GCGACGGATA TAAAACTGAA ACTCCATCAT CATTTAATGA ATTTGGAAGA 
AAAATTTGCG TTCCAATGTG TACACAAGGT TGTGTTAGAG GAACATGCAT AGAACCAAAT 
ATTTGTCGTT GTGACTTTGG TTATGTAGGT GCCAATTGTT CCATTCAGTG CCAATGTAAT 
GGTCATAGTA ATTGTGCAGG CCCAGATAAA TTAGACGTTT GTTTAGAATG TCATAATAAT 
ACAATGGGAC CACAATGTGA CAAATGTAGA CCTTTATTTG TTGGAAATCC AGCAGATAAT 
GGTCAATGTG TACCATGTCT TGAATATTGC AATGGACATA CTCGAATATG TATTAATGAA 
AGCATGACTG TACCGGATCC AAATTCAATT GAAAAAATGT CTATAGAATT ACTAGAAAAA 
CAATTAGTCG AAGGTCCTGT AGCTAAAGCT AAATGCGTAA ATTGTGGAAA TAATACAAGA 
GGTGACAAAT GTGGTGAATG TATGATTGGT TATTTCAGAG GAACAGAAGA TCTTCGTGAT 
ATATGCCGAT CTTGTCAATG TCATGGACAT GGATTTACTT GTGATCCAGT AACAGGAGAA 
AAATGCAATT GTGGTAATAA TACAGAAAGT GATCCAACTT GTACTAGTGG ACCTATGAAA 
AGTACAAATA CTGGTAGTTC CCCATGTTGG ATGGTACAAT GTAGTAAATG TAAAGAAAAT 
TATGCTGGGA CACCAACAAT GGGTCATCAA TGTTATAAAA CTGTAACTGT TGACAACAAA 
ATGTGCTTTG ACTCTAAATT AATAGGAGGC TCTTATGATG AGTGTAAAAT GAAACCAAAA 
CCATTGAATC CCGGTCAAAC GGTATTTTAT ATGGTACAGC CTCGTTTTAT GAATGTTGAT 
ATTAGAGTTA TGGTGGATGT TACTCAAGGG GCTTTAGATC TATTTCTTAG CCCTCGAGAT 
GATTCTTTCG TTGTTACATT AAATTCAACA ACAGGATATC AAGATGTTGA ACTGGACAGT 
AGATTTATGT GGCGAAAAAA TTCACATAAT ATGTGGTTAG ATGAACAAAC TCGTAGTCAA 
ATGAGATTAG TTGAATTCCA ACCTCATAAT ACTTTTTCAT TTGCTTTAAA TGGCACTACA 
ACTGAACCGA CTTGGAATAC TGGCCCTCAG TATATTGTAA TGGAAAGATA TGCAGAAGGT 
TTAGCTACAT TTTTAACAAT CGAGAATAGA AATACATTCT TGGTAGTTAG AAATCTTACA 
AATCGGTTAG TGTTGACATT ACCACAAGAC AAACATGAAC TTCATGAAAC AAAATTTCAT 
ATCGCATTAA GAGCGATTGA TCCTGTGCAT CCAGAAATTA GTGGTCGAGC TGCTTACGGG 
ATGATTTTCT TTCGACAGGA TCAACTTCAT ATTGATTTGT TTGTCTTTTT CTCTGTGTTC 
TTCTCTTGTT TCTTTTTATT TTTAGCCGGT TGTGTAGTTG CATGGAAAAC GAAACAAGCA 
GCGGATGTTC GAAGAGCACG GAGAAGACAT GTTGTCGAAA TGTTGCATAT GGCTAAAAGA 
CCTTTCGCAT CAGCTACCAT CATTTACGAT CGAGATGGTA ATGACTGTAA TCCAAGCTCT 
CCTCAGCGAA AAAGCAGACG TAATAAATTA GTCAGCTTTC ATAGTGATGT TAGACCTGTA 
GCAGTTGAAC CTACTGATGA TGGAGTTGCG GCTGTAGCCA CTGTTTTCAT TAGATTGCCT 
GGAGGTCGAC AAGCTCCAGT TAAATTAGCT CTTGGTAGCT CATTAATACT ATTAACTAGA 
GTTTATCCAG TTAATAGTAG AGTGTTCCTA AGACGTAGAA ACAGTCACGC GTTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026295506.1
CDS	180..9299
Translation	MGWVLSTIIWITIWSCLLRGFELAPQISPKQIPCDKTRKVFTDSWGIISDGPLGSNYTQDSHCEWLIKANNSRQFITLSFRTMGTECSYDYVFVYDGDSFRSPLLGSFSGKTEPQQVTSSSGYMLILLYSDTNYVLDGFHAEFSVTNCSNNCTNHGKCIDNTCFCEDDWGGRDCSRALCPNNCSYSGKCDLKRCHCHNGYSGQSCSLHKMHPESNKWHWLSHSEGGLRPRAAHTAVYIKETDSLYVFGGYDLNYILDNLEVYRFNISQWEDEYGNELEGISSAEHLDPILLALELKGTNTDEKELYGLRTSSFIWKLLSGIKDNNNTFNSRNLANVEKRYGWSEFRIGDREKKIHETKDNETSRSTVRIERNDDLKRKHSRHPRPRYAQTARHRRNLENLDFYKKFHTSNIKSDILNWEEEIAEDSIGDNIYVQEPKIRDELFKDESIKSSIDKLPKPSPRYGHAACRYDGGFVIYGGKVQDGSLSNELWYYNVNTRSWTLRAKNSPFYPPQLTRHTLTLANNYIYLFGGSTVDGEFSSNLYKIKLNLSDPTATTEKWKEVHPRGGKELDVRVVAHSTVYHQATNSLLIYGGVVASVARFSKLSDRMFVFQLDKKVWSEIHYPRAHLRDTYVPRERAFHTCNIIGNYLVVFGGYSHRHNKEEICYDNQMYLYHLGCHAWVSHDVLGLNDKDSRYPKQQGVFAHAADVRNGNTLLLVGGYHGNVNADLLAYTLPPMLAPGDEDNIEPEQICSRHKSLMECTANPECGWCSADEICYGRTIGSNCTTNLQTTRCLGVCPALGDCHSCLIHGQPGGGWGTTARGKKSVSNKLNRGTCTWCVQNARCHHKDDNYGVCGLRDDTLSQIPGWWGSKGTEIIKVEECREMDKRPGLTFLKYKPPVNFTQPDSVTIVNATTVDFSVPSMQGAKTESALGGEMIARLIGFLRPPQDFWDSTLEHLKICVSYNSATLHVSRSDDPQELELVANLTADTSQCIPTKWPDGYQMMLLPGRYLLDFESKRMVTASYAYASKMEIIHNKKTENPKVFTFEYLEPFQNGSCQQYKNCLHCLTDSSCGWCDIINKCLSRSINETESCIADVEWDEDGNPIREWHYLTITPSACANCSNYISCESCVNSNLCEWWTEEARCARIGRLPNAVVSFDQCPIPCRQRSNCTQCLDERGRCVWCEATQECFSFSVYTSEYQFGLCREWMDQAGLMGVTSRSGSSLTGNDQCKSCTRHTNCSSCLHSLSCGWCYSLENPIIGACVQGDFNQPHVNCSLIINEYQNTTLEIDESGWAYAQCPDVDECDLGLHDCHLNAVCTNTHGSFSCQCKRGFNGDGKENCTKTCYEKCVNGYCSEAPDYKCECNLGWTGPDCRTNCGCYNHSTCIQGPGICDKCQDWTTGTYCEECKAGSYGNATTPLGCKKCNCNEHGDKDLGICDRQTGICFCRDNTQGDMCQRCKKGYYGDPRDGGMCYYSCMSRGMLGGEGNGKQGLGSRHSQLSLWESHFGDSPTRECLWIVSPKTDLSSDNMLSGIQNVIQFTIHDDINVSCQENSVYVYDGLPEFVSSVTSHQSQLLGVYCTESTDYPVTVEAKSGFLTVHYKQLDEVEGFNASYLIMTCNNCPGNRECRNGNCLCKAGFVGINCDIEICPNNCTSTKKQGICDKGYGRCVCIPGYGGRDCSIKIKEHQLIFTELFNSEYLADHLDHLRKTLPRFGHTLVADRRGSLWMFGGYSLSHGPLNDIRLFDTKNNTWMPITVESTSKASMPQGRYFHATEIVHSKQQIYIYGGLTMKQEDIQGLSNNTLNDFWKFSLQNQRWMQIIQDKFKKEPPSLAGHTLTLRRNGESESLILIGGFSPKYGYLDTVWEFNLETEAWDTINTIGNGPLGVYGHSTVYHSKSDSFYIFGGYTYAINRTFISNKLYALNYKTRMWSVLPPFDDDLTDGSSLPQARFLHSAVTTDEYMVIFGGRQNPHNTSDSLIAYKYSCNLWIRLITKDMEVIGYPPPPAYAHAMTHADPESNAVYVVGGFDGGIKSHVTLISIPEDLCNLWTDKITCRKYFGCSFCSVITISGNNASFCFSNEISINKDDRCDINVTQAQRSNGIFCNLEWMMSRKCQNFKTCTECLAEWPYYKSEEPVCKWCTNCSNGKCIPSEKDCDELNKCSVRQKSVTDVNQCGERQCPASDCEKCNNLEGCVWTRQVLKTSELGLAVTGEPVYDWNCVPEHIFERSSIKMGSTQCEKRCGDHKDCKNCLKGTGAEGGWRECRWSTQLNECISPSYQPLYCAGGVCGLVLRSSDMDHCPEPCSVFKQCSTCLKHSHCGWCSLDSANITGQGICTEGSLEAPADHPAGGTCEMLYYQQLPTVELLKFLEISDNVTMLIPNVMSKPIFSWHYVRCPPENECMNGHHTCSTKSEKCFDLEEGFECMCGDGYKTETPSSFNEFGRKICVPMCTQGCVRGTCIEPNICRCDFGYVGANCSIQCQCNGHSNCAGPDKLDVCLECHNNTMGPQCDKCRPLFVGNPADNGQCVPCLEYCNGHTRICINESMTVPDPNSIEKMSIELLEKQLVEGPVAKAKCVNCGNNTRGDKCGECMIGYFRGTEDLRDICRSCQCHGHGFTCDPVTGEKCNCGNNTESDPTCTSGPMKSTNTGSSPCWMVQCSKCKENYAGTPTMGHQCYKTVTVDNKMCFDSKLIGGSYDECKMKPKPLNPGQTVFYMVQPRFMNVDIRVMVDVTQGALDLFLSPRDDSFVVTLNSTTGYQDVELDSRFMWRKNSHNMWLDEQTRSQMRLVEFQPHNTFSFALNGTTTEPTWNTGPQYIVMERYAEGLATFLTIENRNTFLVVRNLTNRLVLTLPQDKHELHETKFHIALRAIDPVHPEISGRAAYGMIFFRQDQLHIDLFVFFSVFFSCFFLFLAGCVVAWKTKQAADVRRARRRHVVEMLHMAKRPFASATIIYDRDGNDCNPSSPQRKSRRNKLVSFHSDVRPVAVEPTDDGVAAVATVFIRLPGGRQAPVKLALGSSLILLTRVYPVNSRVFLRRRNSHALN

Target ORF information:

RefSeq Version	XM_026439721.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026439721.1

ORF Insert Sequence:

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGTA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATACA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAACAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTTTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTACGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTATAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTATAT 
CATCAAGCTA CTAATTCTTT ATTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGACTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCAACTGAAA TAGTTCATAG TAAACAACAA 
ATATATATTT ATGGTGGCTT GACAATGAAA CAAGAAGATA TTCAAGGTTT ATCAAATAAC 
ACTCTAAACG ATTTTTGGAA ATTTAGTCTA CAAAATCAAA GATGGATGCA AATCATACAA 
GATAAGTTTA AAAAAGAACC ACCATCTTTA GCTGGACATA CATTAACTCT TCGTAGAAAT 
GGAGAATCAG AAAGTCTAAT ATTAATTGGA GGATTCAGTC CTAAGTATGG TTATCTTGAC 
ACAGTATGGG AATTCAATTT AGAAACAGAA GCTTGGGATA CAATAAATAC AATTGGAAAT 
GGACCATTAG GCGTATATGG CCATTCAACT GTATATCATA GCAAGTCTGA TAGTTTTTAT 
ATTTTCGGTG GATACACTTA TGCGATAAAT CGAACATTTA TTTCTAATAA ATTATACGCA 
TTGAATTATA AAACTCGTAT GTGGTCCGTA CTTCCTCCAT TTGATGATGA TCTCACTGAT 
GGAAGTAGTT TGCCACAAGC TAGATTTCTT CATTCTGCTG TTACCACTGA TGAATATATG 
GTGATATTTG GTGGCCGTCA AAATCCACAT AATACTTCAG ACTCATTGAT TGCTTATAAA 
TATTCATGTA ATTTATGGAT TCGTTTAATA ACAAAAGATA TGGAAGTTAT TGGATATCCA 
CCACCTCCAG CATATGCTCA TGCAATGACT CATGCTGATC CAGAATCTAA TGCTGTATAT 
GTAGTTGGTG GTTTTGATGG TGGAATTAAA AGTCACGTCA CTCTTATAAG TATACCAGAA 
GATTTGTGTA ATCTTTGGAC AGATAAAATA ACTTGCAGAA AATATTTTGG ATGTTCTTTT 
TGTTCTGTTA TTACAATTAG TGGAAATAAT GCTTCATTTT GTTTTTCTAA TGAAATATCA 
ATTAATAAGG ATGATAGATG TGATATTAAT GTTACTCAAG CTCAACGATC AAATGGAATC 
TTTTGTAATT TAGAATGGAT GATGAGTAGA AAATGTCAAA ATTTTAAAAC TTGTACAGAA 
TGTTTAGCAG AATGGCCATA TTATAAAAGT GAAGAACCAG TGTGCAAATG GTGTACAAAT 
TGTTCAAATG GAAAGTGTAT ACCATCTGAG AAAGATTGCG ATGAATTAAA TAAATGCAGT 
GTTAGACAAA AATCAGTAAC AGATGTAAAT CAATGTGGAG AACGTCAATG TCCAGCAAGT 
GATTGCGAGA AATGTAACAA TCTTGAAGGT TGTGTATGGA CAAGGCAGGT TTTGAAAACT 
TCTGAATTGG GACTTGCAGT AACAGGAGAA CCTGTCTATG ATTGGAATTG TGTACCAGAA 
CATATTTTTG AAAGATCAAG TATTAAAATG GGAAGTACGC AATGTGAAAA ACGATGTGGT 
GATCATAAGG ACTGTAAAAA CTGTTTAAAA GGTACAGGAG CTGAAGGTGG TTGGCGAGAA 
TGTAGATGGT CAACGCAACT TAATGAATGT ATTTCTCCAT CATATCAACC TCTGTACTGT 
GCTGGCGGTG TATGTGGTTT GGTATTACGT AGTTCTGATA TGGATCATTG TCCTGAACCA 
TGTTCAGTTT TTAAACAATG CAGTACATGT TTGAAACATT CACACTGTGG ATGGTGTTCT 
TTAGATTCAG CTAATATAAC TGGTCAAGGA ATATGTACAG AAGGTTCATT AGAAGCACCA 
GCAGATCATC CAGCTGGTGG AACATGTGAA ATGTTATATT ATCAACAGTT ACCCACAGTT 
GAACTACTAA AGTTTCTGGA AATCTCAGAC AATGTTACAA TGTTAATTCC AAACGTGATG 
TCAAAACCAA TATTTTCTTG GCATTATGTA CGATGCCCGC CAGAAAATGA ATGTATGAAT 
GGACACCACA CATGTTCCAC AAAAAGTGAA AAATGTTTTG ATTTAGAAGA AGGATTTGAA 
TGCATGTGTG GCGACGGATA TAAAACTGAA ACTCCATCAT CATTTAATGA ATTTGGAAGA 
AAAATTTGCG TTCCAATGTG TACACAAGGT TGTGTTAGAG GAACATGCAT AGAACCAAAT 
ATTTGTCGTT GTGACTTTGG TTATGTAGGT GCCAATTGTT CCATTCAGTG CCAATGTAAT 
GGTCATAGTA ATTGTGCAGG CCCAGATAAA TTAGACGTTT GTTTAGAATG TCATAATAAT 
ACAATGGGAC CACAATGTGA CAAATGTAGA CCTTTATTTG TTGGAAATCC AGCAGATAAT 
GGTCAATGTG TACCATGTCT TGAATATTGC AATGGACATA CTCGAATATG TATTAATGAA 
AGCATGACTG TACCGGATCC AAATTCAATT GAAAAAATGT CTATAGAATT ACTAGAAAAA 
CAATTAGTCG AAGGTCCTGT AGCTAAAGCT AAATGCGTAA ATTGTGGAAA TAATACAAGA 
GGTGACAAAT GTGGTGAATG TATGATTGGT TATTTCAGAG GAACAGAAGA TCTTCGTGAT 
ATATGCCGAT CTTGTCAATG TCATGGACAT GGATTTACTT GTGATCCAGT AACAGGAGAA 
AAATGCAATT GTGGTAATAA TACAGAAAGT GATCCAACTT GTACTAGTGG ACCTATGAAA 
AGTACAAATA CTGGTAGTTC CCCATGTTGG ATGGTACAAT GTAGTAAATG TAAAGAAAAT 
TATGCTGGGA CACCAACAAT GGGTCATCAA TGTTATAAAA CTGTAACTGT TGACAACAAA 
ATGTGCTTTG ACTCTAAATT AATAGGAGGC TCTTATGATG AGTGTAAAAT GAAACCAAAA 
CCATTGAATC CCGGTCAAAC GGTATTTTAT ATGGTACAGC CTCGTTTTAT GAATGTTGAT 
ATTAGAGTTA TGGTGGATGT TACTCAAGGG GCTTTAGATC TATTTCTTAG CCCTCGAGAT 
GATTCTTTCG TTGTTACATT AAATTCAACA ACAGGATATC AAGATGTTGA ACTGGACAGT 
AGATTTATGT GGCGAAAAAA TTCACATAAT ATGTGGTTAG ATGAACAAAC TCGTAGTCAA 
ATGAGATTAG TTGAATTCCA ACCTCATAAT ACTTTTTCAT TTGCTTTAAA TGGCACTACA 
ACTGAACCGA CTTGGAATAC TGGCCCTCAG TATATTGTAA TGGAAAGATA TGCAGAAGGT 
TTAGCTACAT TTTTAACAAT CGAGAATAGA AATACATTCT TGGTAGTTAG AAATCTTACA 
AATCGGTTAG TGTTGACATT ACCACAAGAC AAACATGAAC TTCATGAAAC AAAATTTCAT 
ATCGCATTAA GAGCGATTGA TCCTGTGCAT CCAGAAATTA GTGGTCGAGC TGCTTACGGG 
ATGATTTTCT TTCGACAGGA TCAACTTCAT ATTGATTTGT TTGTCTTTTT CTCTGTGTTC 
TTCTCTTGTT TCTTTTTATT TTTAGCCGGT TGTGTAGTTG CATGGAAAAC GAAACAAGCA 
GCGGATGTTC GAAGAGCACG GAGAAGACAT GTTGTCGAAA TGTTGCATAT GGCTAAAAGA 
CCTTTCGCAT CAGCTACCAT CATTTACGAT CGAGATGGTA ATGACTGTAA TCCAAGCTCT 
CCTCAGCGAA AAAGCAGACG TAATAAATTA GTCAGCTTTC ATAGTGATGT TAGACCTGTA 
GCAGTTGAAC CTACTGATGA TGGAGTTGCG GCTGTAGCCA CTGTTTTCAT TAGATTGCCT 
GGAGGTCGAC AAGCTCCAGT TAAATTAGCT CTTGGTAGCT CATTAATACT ATTAACTAGA 
GTTTATCCAG TTAATAGTAG AGTGTTCCTA AGACGTAGAA ACAGTCACGC GTTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC725895 ( XM_026439724.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026295509.1 Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X5, mRNA.

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CloneID	OHd29708
Clone ID Related Accession (Same CDS sequence)	XM_026439724.1
Accession Version	XM_026439724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	multiple epidermal growth factor-like domains protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037640.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGTA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATACA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAACAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTTTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTACGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTATAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTATAT 
CATCAAGCTA CTAATTCTTT ATTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGACTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCAACTGAAA TAGTTCATAG TAAACAACAA 
ATATATATTT ATGGTGGCTT GACAATGAAA CAAGAAGATA TTCAAGGTTT ATCAAATAAC 
ACTCTAAACG ATTTTTGGAA ATTTAGTCTA CAAAATCAAA GATGGATGCA AATCATACAA 
GATAAGTTTA AAAAAGAACC ACCATCTTTA GCTGGACATA CATTAACTCT TCGTAGAAAT 
GGAGAATCAG AAAGTCTAAT ATTAATTGGA GGATTCAGTC CTAAGTATGG TTATCTTGAC 
ACAGTATGGG AATTCAATTT AGAAACAGAA GCTTGGGATA CAATAAATAC AATTGGAAAT 
GGACCATTAG GCGTATATGG CCATTCAACT GTATATCATA GCAAGTCTGA TAGTTTTTAT 
ATTTTCGGTG GATACACTTA TGCGATAAAT CGAACATTTA TTTCTAATAA ATTATACGCA 
TTGAATTATA AAACTCGTAT GTGGTCCGTA CTTCCTCCAT TTGATGATGA TCTCACTGAT 
GGAAGTAGTT TGCCACAAGC TAGATTTCTT CATTCTGCTG TTACCACTGA TGAATATATG 
GTGATATTTG GTGGCCGTCA AAATCCACAT AATACTTCAG ACTCATTGAT TGCTTATAAA 
TATTCATGTA ATTTATGGAT TCGTTTAATA ACAAAAGATA TGGAAGTTAT TGGATATCCA 
CCACCTCCAG CATATGCTCA TGCAATGACT CATGCTGATC CAGAATCTAA TGCTGTATAT 
GTAGTTGGTG GTTTTGATGG TGGAATTAAA AGTCACGTCA CTCTTATAAG TATACCAGAA 
GATTTGTGTA ATCTTTGGAC AGATAAAATA ACTTGCAGAA AATATTTTGG ATGTTCTTTT 
TGTTCTGTTA TTACAATTAG TGGAAATAAT GCTTCATTTT GTTTTTCTAA TGAAATATCA 
ATTAATAAGG ATGATAGATG TGATATTAAT GTTACTCAAG CTCAACGATC AAATGGAATC 
TTTTGTAATT TAGAATGGAT GATGAGTAGA AAATGTCAAA ATTTTAAAAC TTGTACAGAA 
TGTTTAGCAG AATGGCCATA TTATAAAAGT GAAGAACCAG TGTGCAAATG GTGTACAAAT 
TGTTCAAATG GAAAGTGTAT ACCATCTGAG AAAGATTGCG ATGAATTAAA TAAATGCAGT 
GTTAGACAAA AATCAGTAAC AGATGTAAAT CAATGTGGAG AACGTCAATG TCCAGCAAGT 
GATTGCGAGA AATGTAACAA TCTTGAAGGT TGTGTATGGA CAAGGCAGGT TTTGAAAACT 
TCTGAATTGG GACTTGCAGT AACAGGAGAA CCTGTCTATG ATTGGAATTG TGTACCAGAA 
CATATTTTTG AAAGATCAAG TATTAAAATG GGAAGTACGC AATGTGAAAA ACGATGTGGT 
GATCATAAGG ACTGTAAAAA CTGTTTAAAA GGTACAGGAG CTGAAGGTGG TTGGCGAGAA 
TGTAGATGGT CAACGCAACT TAATGAATGT ATTTCTCCAT CATATCAACC TCTGTACTGT 
GCTGGCGGTG TATGTGGTTT GGTATTACGT AGTTCTGATA TGGATCATTG TCCTGAACCA 
TGTTCAGTTT TTAAACAATG CAGTACATGT TTGAAACATT CACACTGTGG ATGGTGTTCT 
TTAGATTCAG CTAATATAAC TGGTCAAGGA ATATGTACAG AAGGTTCATT AGAAGCACCA 
GCAGATCATC CAGCTGGTGG AACATGTGAA ATGTTATATT ATCAACAGTT ACCCACAGTT 
GAACTACTAA AGTTTCTGGA AATCTCAGAC AATGTTACAA TGTTAATTCC AAACGTGATG 
TCAAAACCAA TATTTTCTTG GCATTATGTA CGATGCCCGC CAGAAAATGA ATGTATGAAT 
GGACACCACA CATGTTCCAC AAAAAGTGAA AAATGTTTTG ATTTAGAAGA AGGATTTGAA 
TGCATGTGTG GCGACGGATA TAAAACTGAA ACTCCATCAT CATTTAATGA ATTTGGAAGA 
AAAATTTGCG TTCCAATGTG TACACAAGGT TGTGTTAGAG GAACATGCAT AGAACCAAAT 
ATTTGTCGTT GTGACTTTGG TTATGTAGGT GCCAATTGTT CCATTCAGTG CCAATGTAAT 
GGTCATAGTA ATTGTGCAGG CCCAGATAAA TTAGACGTTT GTTTAGAATG TCATAATAAT 
ACAATGGGAC CACAATGTGA CAAATGTAGA CCTTTATTTG TTGGAAATCC AGCAGATAAT 
GGTCAATGTG TACCATGTCT TGAATATTGC AATGGACATA CTCGAATATG TATTAATGAA 
AGCATGACTG TACCGGATCC AAATTCAATT GAAAAAATGT CTATAGAATT ACTAGAAAAA 
CAATTAGTCG AAGGTCCTGT AGCTAAAGCT AAATGCGTAA ATTGTGGAAA TAATACAAGA 
GGTGACAAAT GTGGTGAATG TATGATTGGT TATTTCAGAG GAACAGAAGA TCTTCGTGAT 
ATATGCCGAT CTTGTCAATG TCATGGACAT GGATTTACTT GTGATCCAGT AACAGGAGAA 
AAATGCAATT GTGGTAATAA TACAGAAAGT GATCCAACTT GTACTAGTGG ACCTATGAAA 
AGTACAAATA CTGGTAGTTC CCCATGTTGG ATGGTACAAT GTAGTAAATG TAAAGAAAAT 
TATGCTGGGA CACCAACAAT GGGTCATCAA TGTTATAAAA CTGTAACTGT TGACAACAAA 
ATGTGCTTTG ACTCTAAATT AATAGATGAG TGTAAAATGA AACCAAAACC ATTGAATCCC 
GGTCAAACGG TATTTTATAT GGTACAGCCT CGTTTTATGA ATGTTGATAT TAGAGTTATG 
GTGGATGTTA CTCAAGGGGC TTTAGATCTA TTTCTTAGCC CTCGAGATGA TTCTTTCGTT 
GTTACATTAA ATTCAACAAC AGGATATCAA GATGTTGAAC TGGACAGTAG ATTTATGTGG 
CGAAAAAATT CACATAATAT GTGGTTAGAT GAACAAACTC GTAGTCAAAT GAGATTAGTT 
GAATTCCAAC CTCATAATAC TTTTTCATTT GCTTTAAATG GCACTACAAC TGAACCGACT 
TGGAATACTG GCCCTCAGTA TATTGTAATG GAAAGATATG CAGAAGGTTT AGCTACATTT 
TTAACAATCG AGAATAGAAA TACATTCTTG GTAGTTAGAA ATCTTACAAA TCGGTTAGTG 
TTGACATTAC CACAAGACAA ACATGAACTT CATGAAACAA AATTTCATAT CGCATTAAGA 
GCGATTGATC CTGTGCATCC AGAAATTAGT GGTCGAGCTG CTTACGGGAT GATTTTCTTT 
CGACAGGATC AACTTCATAT TGATTTGTTT GTCTTTTTCT CTGTGTTCTT CTCTTGTTTC 
TTTTTATTTT TAGCCGGTTG TGTAGTTGCA TGGAAAACGA AACAAGCAGC GGATGTTCGA 
AGAGCACGGA GAAGACATGT TGTCGAAATG TTGCATATGG CTAAAAGACC TTTCGCATCA 
GCTACCATCA TTTACGATCG AGATGGTAAT GACTGTAATC CAAGCTCTCC TCAGCGAAAA 
AGCAGACGTA ATAAATTAGT CAGCTTTCAT AGTGATGTTA GACCTGTAGC AGTTGAACCT 
ACTGATGATG GAGTTGCGGC TGTAGCCACT GTTTTCATTA GATTGCCTGG AGGTCGACAA 
GCTCCAGTTA AATTAGCTCT TGGTAGCTCA TTAATACTAT TAACTAGAGT TTATCCAGTT 
AATAGTAGAG TGTTCCTAAG ACGTAGAAAC AGTCACGCGT TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026295509.1
CDS	433..9540
Translation	MGWVLSTIIWITIWSCLLRGFELAPQISPKQIPCDKTRKVFTDSWGIISDGPLGSNYTQDSHCEWLIKANNSRQFITLSFRTMGTECSYDYVFVYDGDSFRSPLLGSFSGKTEPQQVTSSSGYMLILLYSDTNYVLDGFHAEFSVTNCSNNCTNHGKCIDNTCFCEDDWGGRDCSRALCPNNCSYSGKCDLKRCHCHNGYSGQSCSLHKMHPESNKWHWLSHSEGGLRPRAAHTAVYIKETDSLYVFGGYDLNYILDNLEVYRFNISQWEDEYGNELEGISSAEHLDPILLALELKGTNTDEKELYGLRTSSFIWKLLSGIKDNNNTFNSRNLANVEKRYGWSEFRIGDREKKIHETKDNETSRSTVRIERNDDLKRKHSRHPRPRYAQTARHRRNLENLDFYKKFHTSNIKSDILNWEEEIAEDSIGDNIYVQEPKIRDELFKDESIKSSIDKLPKPSPRYGHAACRYDGGFVIYGGKVQDGSLSNELWYYNVNTRSWTLRAKNSPFYPPQLTRHTLTLANNYIYLFGGSTVDGEFSSNLYKIKLNLSDPTATTEKWKEVHPRGGKELDVRVVAHSTVYHQATNSLLIYGGVVASVARFSKLSDRMFVFQLDKKVWSEIHYPRAHLRDTYVPRERAFHTCNIIGNYLVVFGGYSHRHNKEEICYDNQMYLYHLGCHAWVSHDVLGLNDKDSRYPKQQGVFAHAADVRNGNTLLLVGGYHGNVNADLLAYTLPPMLAPGDEDNIEPEQICSRHKSLMECTANPECGWCSADEICYGRTIGSNCTTNLQTTRCLGVCPALGDCHSCLIHGQPGGGWGTTARGKKSVSNKLNRGTCTWCVQNARCHHKDDNYGVCGLRDDTLSQIPGWWGSKGTEIIKVEECREMDKRPGLTFLKYKPPVNFTQPDSVTIVNATTVDFSVPSMQGAKTESALGGEMIARLIGFLRPPQDFWDSTLEHLKICVSYNSATLHVSRSDDPQELELVANLTADTSQCIPTKWPDGYQMMLLPGRYLLDFESKRMVTASYAYASKMEIIHNKKTENPKVFTFEYLEPFQNGSCQQYKNCLHCLTDSSCGWCDIINKCLSRSINETESCIADVEWDEDGNPIREWHYLTITPSACANCSNYISCESCVNSNLCEWWTEEARCARIGRLPNAVVSFDQCPIPCRQRSNCTQCLDERGRCVWCEATQECFSFSVYTSEYQFGLCREWMDQAGLMGVTSRSGSSLTGNDQCKSCTRHTNCSSCLHSLSCGWCYSLENPIIGACVQGDFNQPHVNCSLIINEYQNTTLEIDESGWAYAQCPDVDECDLGLHDCHLNAVCTNTHGSFSCQCKRGFNGDGKENCTKTCYEKCVNGYCSEAPDYKCECNLGWTGPDCRTNCGCYNHSTCIQGPGICDKCQDWTTGTYCEECKAGSYGNATTPLGCKKCNCNEHGDKDLGICDRQTGICFCRDNTQGDMCQRCKKGYYGDPRDGGMCYYSCMSRGMLGGEGNGKQGLGSRHSQLSLWESHFGDSPTRECLWIVSPKTDLSSDNMLSGIQNVIQFTIHDDINVSCQENSVYVYDGLPEFVSSVTSHQSQLLGVYCTESTDYPVTVEAKSGFLTVHYKQLDEVEGFNASYLIMTCNNCPGNRECRNGNCLCKAGFVGINCDIEICPNNCTSTKKQGICDKGYGRCVCIPGYGGRDCSIKIKEHQLIFTELFNSEYLADHLDHLRKTLPRFGHTLVADRRGSLWMFGGYSLSHGPLNDIRLFDTKNNTWMPITVESTSKASMPQGRYFHATEIVHSKQQIYIYGGLTMKQEDIQGLSNNTLNDFWKFSLQNQRWMQIIQDKFKKEPPSLAGHTLTLRRNGESESLILIGGFSPKYGYLDTVWEFNLETEAWDTINTIGNGPLGVYGHSTVYHSKSDSFYIFGGYTYAINRTFISNKLYALNYKTRMWSVLPPFDDDLTDGSSLPQARFLHSAVTTDEYMVIFGGRQNPHNTSDSLIAYKYSCNLWIRLITKDMEVIGYPPPPAYAHAMTHADPESNAVYVVGGFDGGIKSHVTLISIPEDLCNLWTDKITCRKYFGCSFCSVITISGNNASFCFSNEISINKDDRCDINVTQAQRSNGIFCNLEWMMSRKCQNFKTCTECLAEWPYYKSEEPVCKWCTNCSNGKCIPSEKDCDELNKCSVRQKSVTDVNQCGERQCPASDCEKCNNLEGCVWTRQVLKTSELGLAVTGEPVYDWNCVPEHIFERSSIKMGSTQCEKRCGDHKDCKNCLKGTGAEGGWRECRWSTQLNECISPSYQPLYCAGGVCGLVLRSSDMDHCPEPCSVFKQCSTCLKHSHCGWCSLDSANITGQGICTEGSLEAPADHPAGGTCEMLYYQQLPTVELLKFLEISDNVTMLIPNVMSKPIFSWHYVRCPPENECMNGHHTCSTKSEKCFDLEEGFECMCGDGYKTETPSSFNEFGRKICVPMCTQGCVRGTCIEPNICRCDFGYVGANCSIQCQCNGHSNCAGPDKLDVCLECHNNTMGPQCDKCRPLFVGNPADNGQCVPCLEYCNGHTRICINESMTVPDPNSIEKMSIELLEKQLVEGPVAKAKCVNCGNNTRGDKCGECMIGYFRGTEDLRDICRSCQCHGHGFTCDPVTGEKCNCGNNTESDPTCTSGPMKSTNTGSSPCWMVQCSKCKENYAGTPTMGHQCYKTVTVDNKMCFDSKLIDECKMKPKPLNPGQTVFYMVQPRFMNVDIRVMVDVTQGALDLFLSPRDDSFVVTLNSTTGYQDVELDSRFMWRKNSHNMWLDEQTRSQMRLVEFQPHNTFSFALNGTTTEPTWNTGPQYIVMERYAEGLATFLTIENRNTFLVVRNLTNRLVLTLPQDKHELHETKFHIALRAIDPVHPEISGRAAYGMIFFRQDQLHIDLFVFFSVFFSCFFLFLAGCVVAWKTKQAADVRRARRRHVVEMLHMAKRPFASATIIYDRDGNDCNPSSPQRKSRRNKLVSFHSDVRPVAVEPTDDGVAAVATVFIRLPGGRQAPVKLALGSSLILLTRVYPVNSRVFLRRRNSHALN

Target ORF information:

RefSeq Version	XM_026439724.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera multiple EGF-like-domains 8 (LOC725895), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026439724.1

ORF Insert Sequence:

ATGGGGTGGG TTTTGTCCAC CATAATATGG ATTACTATAT GGAGTTGCCT CTTGCGGGGC 
TTCGAATTAG CTCCACAGAT ATCTCCAAAA CAAATTCCTT GTGATAAAAC AAGAAAAGTT 
TTTACTGATT CTTGGGGAAT TATTAGTGAT GGACCATTGG GCTCAAATTA TACTCAAGAT 
TCTCATTGTG AATGGCTTAT AAAAGCAAAT AATAGTCGAC AATTTATTAC TTTAAGTTTT 
CGTACAATGG GAACAGAATG TAGCTATGAT TATGTTTTTG TTTATGATGG AGATTCTTTT 
CGATCTCCTC TTTTAGGCAG TTTTAGTGGT AAAACTGAAC CACAACAAGT AACATCATCA 
TCTGGTTATA TGTTAATACT ATTGTATAGT GATACAAACT ATGTTTTGGA TGGTTTTCAT 
GCGGAATTTT CAGTTACAAA TTGTTCAAAC AATTGTACAA ATCATGGCAA ATGTATTGAT 
AATACATGCT TTTGTGAGGA TGATTGGGGT GGACGAGATT GTTCTAGAGC TCTTTGTCCA 
AATAATTGTA GTTATAGTGG AAAATGTGAT TTAAAACGAT GTCACTGTCA CAATGGATAT 
TCAGGACAAT CTTGTTCATT ACATAAAATG CATCCTGAAA GCAACAAATG GCACTGGCTT 
TCTCATTCAG AAGGTGGATT AAGGCCACGG GCAGCACATA CTGCAGTTTA TATAAAAGAA 
ACTGATTCTC TTTATGTTTT TGGAGGCTAT GATCTCAATT ATATACTTGA TAATTTGGAA 
GTGTATCGAT TTAATATAAG TCAATGGGAA GACGAATATG GTAATGAATT AGAAGGCATC 
TCATCTGCAG AACATTTAGA TCCTATACTC CTTGCCCTTG AATTAAAAGG AACAAATACA 
GATGAAAAAG AATTATATGG TCTTCGTACT TCGTCTTTCA TATGGAAATT GCTTTCTGGT 
ATTAAAGATA ATAACAATAC CTTTAATTCA CGTAATCTTG CTAATGTAGA AAAAAGATAT 
GGCTGGTCAG AATTTAGGAT TGGAGATCGT GAAAAAAAAA TTCATGAAAC TAAAGATAAT 
GAAACTAGTA GAAGTACAGT TAGAATAGAA AGAAATGATG ATCTTAAAAG AAAACATTCT 
AGACATCCTA GACCTCGATA TGCACAAACT GCAAGACACC GTAGAAATCT TGAAAACCTA 
GATTTTTATA AAAAATTTCA TACTTCAAAT ATAAAAAGTG ATATATTAAA TTGGGAAGAG 
GAAATTGCAG AAGATTCTAT TGGAGATAAT ATTTACGTTC AAGAACCTAA AATAAGAGAC 
GAATTATTTA AAGATGAATC TATTAAATCA TCAATTGATA AACTACCTAA ACCAAGTCCG 
CGATATGGCC ATGCTGCTTG TAGATATGAT GGTGGTTTTG TTATATATGG TGGAAAAGTA 
CAAGATGGTT CTTTATCTAA TGAATTATGG TATTATAATG TTAATACACG TTCCTGGACA 
TTAAGAGCAA AAAATTCTCC ATTTTATCCT CCACAATTAA CAAGACATAC ACTTACTTTA 
GCAAATAATT ATATTTATCT TTTTGGTGGT AGTACAGTTG ATGGTGAATT TTCATCAAAT 
TTATATAAGA TTAAATTAAA TTTATCTGAC CCTACAGCAA CTACAGAAAA ATGGAAAGAA 
GTACATCCTC GAGGAGGTAA AGAGTTAGAT GTACGTGTAG TTGCACATTC AACTGTATAT 
CATCAAGCTA CTAATTCTTT ATTAATTTAT GGAGGAGTAG TAGCCAGTGT AGCACGATTT 
AGTAAATTAT CAGATAGGAT GTTTGTATTT CAACTTGACA AAAAAGTTTG GTCGGAAATT 
CATTATCCAA GAGCACATTT AAGAGATACT TATGTTCCAA GAGAACGAGC ATTTCATACC 
TGTAATATTA TAGGAAATTA CTTAGTTGTA TTTGGCGGAT ATTCTCATAG ACATAATAAA 
GAAGAAATAT GTTATGATAA TCAGATGTAT CTTTATCATT TAGGCTGTCA CGCTTGGGTA 
AGCCATGATG TATTAGGTTT AAATGATAAA GACTCTCGTT ATCCAAAACA ACAGGGAGTA 
TTTGCACATG CAGCAGATGT ACGGAATGGA AATACATTGT TATTAGTTGG TGGTTATCAT 
GGAAATGTAA ATGCTGATTT ACTTGCATAT ACATTACCAC CAATGCTTGC TCCAGGAGAT 
GAAGATAATA TAGAACCTGA GCAAATTTGT TCAAGACATA AAAGTTTGAT GGAATGTACA 
GCTAATCCAG AATGTGGTTG GTGTTCGGCA GATGAGATAT GTTACGGTAG GACTATTGGT 
AGTAATTGTA CAACAAATCT TCAAACGACG CGTTGTTTAG GTGTTTGTCC TGCGCTTGGA 
GATTGTCATT CTTGTTTAAT ACATGGTCAA CCTGGTGGAG GGTGGGGTAC AACTGCACGT 
GGAAAAAAGT CTGTGTCTAA TAAGTTAAAT CGTGGAACTT GTACTTGGTG TGTTCAAAAT 
GCACGTTGTC ATCATAAAGA TGATAATTAT GGTGTTTGTG GACTTCGAGA TGATACATTA 
TCTCAAATAC CGGGATGGTG GGGATCGAAA GGTACAGAAA TTATAAAAGT TGAAGAATGT 
CGGGAAATGG ATAAAAGACC AGGATTAACA TTTTTAAAAT ATAAACCTCC AGTAAATTTT 
ACTCAACCTG ATTCAGTTAC GATAGTTAAT GCTACTACAG TAGATTTTAG TGTTCCATCT 
ATGCAAGGAG CAAAAACAGA ATCAGCACTT GGTGGAGAAA TGATTGCTAG ATTAATTGGT 
TTTCTTAGAC CACCTCAAGA TTTTTGGGAT AGTACACTAG AACATTTGAA AATTTGTGTT 
AGCTACAATA GTGCAACATT ACATGTATCA AGAAGTGATG ATCCTCAAGA ATTGGAATTA 
GTTGCAAATT TAACTGCCGA TACATCGCAA TGTATTCCAA CAAAATGGCC AGATGGATAT 
CAAATGATGT TATTACCAGG TCGATATTTA TTAGATTTTG AATCAAAAAG AATGGTTACT 
GCAAGTTATG CATATGCTAG TAAAATGGAA ATTATTCATA ACAAAAAAAC CGAGAATCCA 
AAAGTATTTA CATTTGAATA TCTCGAACCA TTTCAAAACG GTTCTTGTCA GCAATATAAA 
AACTGCCTTC ATTGCTTAAC TGATTCTTCA TGCGGTTGGT GCGATATTAT TAATAAATGT 
CTTTCACGTT CAATTAACGA AACAGAAAGC TGCATAGCTG ATGTAGAATG GGATGAAGAT 
GGGAATCCTA TTCGCGAATG GCATTATTTA ACTATAACTC CTTCAGCTTG TGCTAATTGT 
TCTAATTATA TATCATGTGA ATCTTGTGTT AATAGTAATT TGTGTGAATG GTGGACAGAA 
GAAGCTCGTT GCGCAAGAAT AGGTAGATTG CCTAATGCAG TTGTTAGCTT TGATCAATGT 
CCAATTCCAT GTAGACAACG TTCAAATTGC ACACAATGTT TAGATGAGCG TGGAAGATGT 
GTTTGGTGTG AGGCCACACA GGAATGTTTT TCTTTCTCAG TATATACATC TGAATATCAA 
TTTGGTTTAT GTCGAGAATG GATGGATCAG GCTGGTTTAA TGGGAGTAAC ATCAAGGTCA 
GGATCATCCT TAACAGGCAA TGATCAATGC AAAAGTTGTA CTCGACACAC AAATTGTTCT 
AGCTGTTTAC ATTCATTGAG TTGTGGTTGG TGTTACAGTT TAGAAAATCC TATTATAGGA 
GCATGTGTTC AAGGAGATTT CAATCAACCA CATGTCAATT GTAGTTTAAT TATCAATGAA 
TATCAAAATA CAACCTTAGA GATTGATGAA TCAGGTTGGG CATATGCTCA ATGTCCAGAT 
GTTGACGAAT GTGACTTAGG TTTACATGAT TGTCATCTTA ATGCAGTATG CACAAATACT 
CATGGTAGTT TTAGCTGTCA ATGTAAAAGA GGTTTTAATG GAGATGGCAA AGAAAATTGT 
ACTAAGACTT GTTATGAAAA ATGTGTTAAT GGTTATTGTA GTGAAGCTCC TGATTATAAA 
TGTGAATGTA ATTTAGGATG GACAGGACCA GATTGTAGAA CAAATTGTGG TTGTTATAAT 
CACTCTACTT GCATACAAGG ACCAGGTATT TGTGATAAAT GTCAAGATTG GACTACTGGT 
ACGTATTGTG AAGAATGTAA AGCAGGCAGT TATGGTAATG CTACAACACC TCTTGGATGC 
AAAAAGTGCA ATTGTAATGA ACATGGAGAT AAAGACTTAG GAATCTGCGA TCGACAAACT 
GGTATATGTT TTTGCCGTGA TAATACGCAA GGGGATATGT GTCAACGTTG TAAGAAAGGC 
TATTACGGCG ATCCAAGAGA TGGCGGAATG TGCTATTATA GTTGTATGTC CCGAGGAATG 
CTTGGAGGAG AAGGAAATGG CAAACAAGGT CTTGGCAGCA GACACTCACA ATTATCATTA 
TGGGAAAGTC ATTTTGGAGA TTCTCCAACA AGAGAGTGTC TTTGGATAGT TAGTCCAAAA 
ACAGATCTTT CATCAGACAA CATGCTCTCA GGAATACAAA ATGTAATACA ATTTACTATA 
CATGATGATA TTAATGTTAG TTGTCAAGAA AATAGTGTTT ATGTATATGA TGGACTTCCC 
GAATTTGTTT CATCAGTGAC TAGTCATCAG AGTCAACTTT TAGGTGTTTA TTGTACAGAA 
AGTACAGATT ATCCTGTTAC TGTAGAAGCT AAATCAGGAT TTCTTACTGT TCATTATAAA 
CAATTGGATG AAGTAGAAGG CTTCAATGCA AGTTATTTAA TAATGACTTG TAATAATTGT 
CCTGGAAATC GGGAATGTCG AAATGGTAAT TGCTTGTGTA AAGCTGGTTT TGTAGGAATC 
AATTGCGATA TTGAAATATG TCCTAATAAT TGCACTTCAA CTAAGAAACA AGGGATTTGC 
GATAAAGGTT ATGGCCGTTG TGTCTGTATA CCTGGTTATG GAGGACGTGA TTGCTCAATT 
AAAATTAAAG AACATCAATT AATTTTTACC GAACTGTTTA ACTCAGAATA TTTAGCTGAT 
CATTTGGATC ATTTGAGAAA AACTTTACCA CGATTTGGTC ATACATTGGT TGCTGATAGA 
AGGGGTAGTT TATGGATGTT TGGAGGCTAT TCTCTTAGTC ATGGACCTCT AAATGATATT 
AGACTTTTTG ATACTAAAAA TAACACATGG ATGCCTATTA CTGTAGAATC TACTTCAAAG 
GCTTCTATGC CTCAAGGTAG ATATTTCCAT GCAACTGAAA TAGTTCATAG TAAACAACAA 
ATATATATTT ATGGTGGCTT GACAATGAAA CAAGAAGATA TTCAAGGTTT ATCAAATAAC 
ACTCTAAACG ATTTTTGGAA ATTTAGTCTA CAAAATCAAA GATGGATGCA AATCATACAA 
GATAAGTTTA AAAAAGAACC ACCATCTTTA GCTGGACATA CATTAACTCT TCGTAGAAAT 
GGAGAATCAG AAAGTCTAAT ATTAATTGGA GGATTCAGTC CTAAGTATGG TTATCTTGAC 
ACAGTATGGG AATTCAATTT AGAAACAGAA GCTTGGGATA CAATAAATAC AATTGGAAAT 
GGACCATTAG GCGTATATGG CCATTCAACT GTATATCATA GCAAGTCTGA TAGTTTTTAT 
ATTTTCGGTG GATACACTTA TGCGATAAAT CGAACATTTA TTTCTAATAA ATTATACGCA 
TTGAATTATA AAACTCGTAT GTGGTCCGTA CTTCCTCCAT TTGATGATGA TCTCACTGAT 
GGAAGTAGTT TGCCACAAGC TAGATTTCTT CATTCTGCTG TTACCACTGA TGAATATATG 
GTGATATTTG GTGGCCGTCA AAATCCACAT AATACTTCAG ACTCATTGAT TGCTTATAAA 
TATTCATGTA ATTTATGGAT TCGTTTAATA ACAAAAGATA TGGAAGTTAT TGGATATCCA 
CCACCTCCAG CATATGCTCA TGCAATGACT CATGCTGATC CAGAATCTAA TGCTGTATAT 
GTAGTTGGTG GTTTTGATGG TGGAATTAAA AGTCACGTCA CTCTTATAAG TATACCAGAA 
GATTTGTGTA ATCTTTGGAC AGATAAAATA ACTTGCAGAA AATATTTTGG ATGTTCTTTT 
TGTTCTGTTA TTACAATTAG TGGAAATAAT GCTTCATTTT GTTTTTCTAA TGAAATATCA 
ATTAATAAGG ATGATAGATG TGATATTAAT GTTACTCAAG CTCAACGATC AAATGGAATC 
TTTTGTAATT TAGAATGGAT GATGAGTAGA AAATGTCAAA ATTTTAAAAC TTGTACAGAA 
TGTTTAGCAG AATGGCCATA TTATAAAAGT GAAGAACCAG TGTGCAAATG GTGTACAAAT 
TGTTCAAATG GAAAGTGTAT ACCATCTGAG AAAGATTGCG ATGAATTAAA TAAATGCAGT 
GTTAGACAAA AATCAGTAAC AGATGTAAAT CAATGTGGAG AACGTCAATG TCCAGCAAGT 
GATTGCGAGA AATGTAACAA TCTTGAAGGT TGTGTATGGA CAAGGCAGGT TTTGAAAACT 
TCTGAATTGG GACTTGCAGT AACAGGAGAA CCTGTCTATG ATTGGAATTG TGTACCAGAA 
CATATTTTTG AAAGATCAAG TATTAAAATG GGAAGTACGC AATGTGAAAA ACGATGTGGT 
GATCATAAGG ACTGTAAAAA CTGTTTAAAA GGTACAGGAG CTGAAGGTGG TTGGCGAGAA 
TGTAGATGGT CAACGCAACT TAATGAATGT ATTTCTCCAT CATATCAACC TCTGTACTGT 
GCTGGCGGTG TATGTGGTTT GGTATTACGT AGTTCTGATA TGGATCATTG TCCTGAACCA 
TGTTCAGTTT TTAAACAATG CAGTACATGT TTGAAACATT CACACTGTGG ATGGTGTTCT 
TTAGATTCAG CTAATATAAC TGGTCAAGGA ATATGTACAG AAGGTTCATT AGAAGCACCA 
GCAGATCATC CAGCTGGTGG AACATGTGAA ATGTTATATT ATCAACAGTT ACCCACAGTT 
GAACTACTAA AGTTTCTGGA AATCTCAGAC AATGTTACAA TGTTAATTCC AAACGTGATG 
TCAAAACCAA TATTTTCTTG GCATTATGTA CGATGCCCGC CAGAAAATGA ATGTATGAAT 
GGACACCACA CATGTTCCAC AAAAAGTGAA AAATGTTTTG ATTTAGAAGA AGGATTTGAA 
TGCATGTGTG GCGACGGATA TAAAACTGAA ACTCCATCAT CATTTAATGA ATTTGGAAGA 
AAAATTTGCG TTCCAATGTG TACACAAGGT TGTGTTAGAG GAACATGCAT AGAACCAAAT 
ATTTGTCGTT GTGACTTTGG TTATGTAGGT GCCAATTGTT CCATTCAGTG CCAATGTAAT 
GGTCATAGTA ATTGTGCAGG CCCAGATAAA TTAGACGTTT GTTTAGAATG TCATAATAAT 
ACAATGGGAC CACAATGTGA CAAATGTAGA CCTTTATTTG TTGGAAATCC AGCAGATAAT 
GGTCAATGTG TACCATGTCT TGAATATTGC AATGGACATA CTCGAATATG TATTAATGAA 
AGCATGACTG TACCGGATCC AAATTCAATT GAAAAAATGT CTATAGAATT ACTAGAAAAA 
CAATTAGTCG AAGGTCCTGT AGCTAAAGCT AAATGCGTAA ATTGTGGAAA TAATACAAGA 
GGTGACAAAT GTGGTGAATG TATGATTGGT TATTTCAGAG GAACAGAAGA TCTTCGTGAT 
ATATGCCGAT CTTGTCAATG TCATGGACAT GGATTTACTT GTGATCCAGT AACAGGAGAA 
AAATGCAATT GTGGTAATAA TACAGAAAGT GATCCAACTT GTACTAGTGG ACCTATGAAA 
AGTACAAATA CTGGTAGTTC CCCATGTTGG ATGGTACAAT GTAGTAAATG TAAAGAAAAT 
TATGCTGGGA CACCAACAAT GGGTCATCAA TGTTATAAAA CTGTAACTGT TGACAACAAA 
ATGTGCTTTG ACTCTAAATT AATAGATGAG TGTAAAATGA AACCAAAACC ATTGAATCCC 
GGTCAAACGG TATTTTATAT GGTACAGCCT CGTTTTATGA ATGTTGATAT TAGAGTTATG 
GTGGATGTTA CTCAAGGGGC TTTAGATCTA TTTCTTAGCC CTCGAGATGA TTCTTTCGTT 
GTTACATTAA ATTCAACAAC AGGATATCAA GATGTTGAAC TGGACAGTAG ATTTATGTGG 
CGAAAAAATT CACATAATAT GTGGTTAGAT GAACAAACTC GTAGTCAAAT GAGATTAGTT 
GAATTCCAAC CTCATAATAC TTTTTCATTT GCTTTAAATG GCACTACAAC TGAACCGACT 
TGGAATACTG GCCCTCAGTA TATTGTAATG GAAAGATATG CAGAAGGTTT AGCTACATTT 
TTAACAATCG AGAATAGAAA TACATTCTTG GTAGTTAGAA ATCTTACAAA TCGGTTAGTG 
TTGACATTAC CACAAGACAA ACATGAACTT CATGAAACAA AATTTCATAT CGCATTAAGA 
GCGATTGATC CTGTGCATCC AGAAATTAGT GGTCGAGCTG CTTACGGGAT GATTTTCTTT 
CGACAGGATC AACTTCATAT TGATTTGTTT GTCTTTTTCT CTGTGTTCTT CTCTTGTTTC 
TTTTTATTTT TAGCCGGTTG TGTAGTTGCA TGGAAAACGA AACAAGCAGC GGATGTTCGA 
AGAGCACGGA GAAGACATGT TGTCGAAATG TTGCATATGG CTAAAAGACC TTTCGCATCA 
GCTACCATCA TTTACGATCG AGATGGTAAT GACTGTAATC CAAGCTCTCC TCAGCGAAAA 
AGCAGACGTA ATAAATTAGT CAGCTTTCAT AGTGATGTTA GACCTGTAGC AGTTGAACCT 
ACTGATGATG GAGTTGCGGC TGTAGCCACT GTTTTCATTA GATTGCCTGG AGGTCGACAA 
GCTCCAGTTA AATTAGCTCT TGGTAGCTCA TTAATACTAT TAACTAGAGT TTATCCAGTT 
AATAGTAGAG TGTTCCTAAG ACGTAGAAAC AGTCACGCGT TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC725895 cDNA ORF clone, Apis mellifera(honey bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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