LOC725591 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC725591
Entrez Gene ID	725591
Full Name	protein crumbs
Synonyms	crb
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_026439374.1	XP_026295159.1	protein crumbs isoform X3
XM_006569849.2	XP_006569912.2	protein crumbs
XM_026439371.1	XP_026295156.1	protein crumbs isoform X1
XM_026439372.1	XP_026295157.1	protein crumbs isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC725591 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC725591 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHd21274	XM_006569849.2 Latest version!	Apis mellifera protein crumbs (LOC725591), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHd29285	XM_026439371.1 Latest version!	Apis mellifera protein crumbs (LOC725591), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHd29286	XM_026439372.1 Latest version!	Apis mellifera protein crumbs (LOC725591), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHd29287	XM_026439374.1 Latest version!	Apis mellifera protein crumbs (LOC725591), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC725591 ( XM_006569849.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006569912.2 Apis mellifera protein crumbs (LOC725591), mRNA.

Price & Availability↑

CloneID	OHd21274
Clone ID Related Accession (Same CDS sequence)	XM_006569849.2
Accession Version	XM_006569849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463932800000
Organism	Apis mellifera(honey bee)
Product	protein crumbs
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007072.3) and transcript sequence (GAZV01035273.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006569849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 371 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCGGGCGC GATACGTCGT GGCAGTACTT ACAAGTTTAT TAGTAGTGAC CGACGAAGCG 
CCCCAAGATA TCAGACACAA GAATCACGAT GAACGGCCCA GAGAAGCTTA TTTCAATGGA 
TCGGCATTCC TGAAATTGAG CTCGTTCGTA TCTGTGCACA GGCACAGCGG CTTGAGCTTT 
CGAACTTGCG AGGGCGGCCG GCTCTTCACC CAAAAGTACG ACGAGGACTC GATCAGTCTG 
GAAGTGACCC CGGATGGACT TTTGTTCGCG GCGATTGTCG AGCAGCAGAG ATACAAAGCG 
AGATTGAACG CCAGGCTGTT GAACAACGCC TGGCACAATG TCAATCTATT CTTTAGACTC 
GGAAATCTCA CCTTGAACGC AGCGGGCCAC ACTCAGGTGA TCGCCAACGC GACTTACAAC 
GCTGCCATTC TCACGTTGCC CGATTACGAT ATGAACGGGT CTCTCGTCGT TGGAGAAGGG 
TTCAAAGGAT GTATCCTGCA GGGTCCGGGG ATTCTTTTTA ACGACACGAT GAATAACGGG 
GCAGTTTTTG GCCCCTGCCC CGCGGAAAAT AAAGGATGTG GTACGAGCGG ACTTAACGGT 
GGTGTAGGAT CAAGCGTAAT CACCACCATG GATTTCTGCT ATCTCGAGCC ATGCATGATG 
CATGGTAAAT GCGTGTCACG GCAGGATCGT TACGAGTGTC ACTGTTACGC CCGATATTCA 
GGCAACAACT GTCAAATTGA CAACGGTCCG CCATGCTTGA GTGGGCCATG CCGGAACGGT 
GGTACGTGCA ACGAGGATAT AAAAGGGGAT TTCAGCTGCG CCTGCAAGCC GGGCTTCACC 
GGTGTTCACT GCGAGTCGCA GCTCGGCGTA AGGCTGTGCG AGCAAAGTCC GTGCAGGAAC 
GACGGTGTTT GTTTAGCGGT GACCGAGACC GAATATAAAT GCGATTGCCA GCCGGGATGG 
AGTGGAAAAA ATTGCGAAAC GAATATCAAC GAGTGCTCCC CGAATCCTTG CAGGCACGGC 
GGTGTTTGTA TCGATGGTAT TAATAATTAT ACGTGCATAT GCGACAGGAC TGGATACGAA 
GGTGCGAATT GCGAGGTGGA CATAGACGAA TGCTTGGCTA ATCCTTGCTT GAACAACGGC 
GTGTGCTACG ACAATTACGG CGGTTACATA TGCCACTGCC CGAATGGTTT CGAGGGGCAG 
AACTGCGAGT TGAACTTGAA CGAGTGCTTG TCGAATCCTT GCAAACATGG CGGCGATTGC 
GTGGACGACG TCGGCTCGTA TCACTGCAAT TGCCCGTCCG GATACGCCGG CCGGCACTGC 
GAGCTTAGAA GTTTGTGCGA GAACGCGGCT TGCCCCTCGA ACAGCATCTG CGTGGAGGAC 
GCGCACGGGC CGCAATGCGT GTGTAATCCC GGATACATGG GCAATCCCCC GAATTGCACC 
ATCAATTATT GCGCCAATAA TCCGTGCAGC AACGGGGGCA CTTGCACGAG CAACAAAGAC 
GGATTCAATT GCACTTGCCC ACCGGAATGG AAAGGAACGA CATGCCTGTC ATCCGCCTCG 
GATTGGTGTT CGGCTTGTTA CAATGGAGGC AGTTGTCTCG AGACGCGTTT CGGTATTATG 
TGTCAGTGCC CAAGATTTTG GACGGGACCG CAATGCAAGG AACCGATCAC CTGTCGCGAT 
CTACCTTGCA AACAAGCTTC CGCTTGTCAC GATTATCCCG GCGGTTATTA TTGTACCTGT 
GAGGCAGGAT GGACGGGACC TGAATGTTCC ATCGACGTGG ACGAGTGTTC CAGCGATCCT 
TGCCGTAACG GCGGTATCTG CATTGATCAA CAAAATAGCT ATTATTGTCA ATGTCTTCCA 
GGATACACCG GTAAAAATTG CCAGATCAAC GTGGACGAGT GTTTGTCCCA GCCCTGTCAA 
AATGGCGGCA CTTGCATCGA TCGAATCAAC GGATACATAT GTAACTGCAC GAAAGACTTC 
ATGGATGAAA ATTGCGAGCG GGAGTACAAC GCTTGCGCAG CAAATCCTTG CCACAACAAC 
GGGAACTGCA CGTTGATACC GAGATCTCGG CGTGAATTTG TCTGCGAGTG TCCACGTGGC 
TTCGAAGGGA AAACTTGCGA CGTAAACGTG GACGATTGCA TCGACGTGAT ATGCCCCGAT 
GGGCAAGTTT GCGTCGACGG TATCGCCGGT TACGAGTGCA AATGTCGCGA GGGTTACAGA 
GATCCTAATT GCACTCACAT CGTCGACCAT TGCGCGTCAG TGTCTTGTAA CAGCGGTACG 
TGCGTAGATC TTGGAGATCA AGGATACGAG TGCAAGTGCA AGGATGGTTT CCAAGGAAAA 
TTTTGCGACG AAGATGTGAA CGAATGCAAC GTGGAGGGCA GTTCCTTGTG CAATAACGGT 
ATTTGCGTAA ATACAGACGG CAGCTACAAT TGTTTCTGCA GGCCAGGATT TTCCGGGGAT 
CACTGCGACA TCGACATAGA TGAATGCTTG TGCGGCCCGT GCAAGAATAA TGCCACGTGT 
ATCGACGGTA TCAATACGTT CGAATGCCAG TGCCCGCTTG GTTATTCCGG GAAAACGTGC 
GACGTGGACG TAAACGAGTG CGAAAGTGAT CCCTGTTTAA ACGGTGCGAC TTGCGTCGAT 
GAAATAGCCA GTTACATGTG CATTTGTTCA CCCGGTTTCC GGGGGTTAAA CTGCGAGATA 
AACATCGACG ATTGCGAACC CCTGCCGTGT TTAAACCACG GCCAATGCAT CGACGGTATA 
AACAATTATA CCTGCGACTG TAGCAATACC GGGTTCGAGG GGGCGCATTG CGAGAAAAAT 
ATCGACGATT GCCGGTCCGG CCCATGCGTG AACGGTGCTC ATTGCATAGA CGATATCAAG 
AATTACAAAT GTCAGTGCTA CGCCGGTTAT ACCGGGAAAA ATTGCGAAGT CGATATAAAC 
GAGTGCGAGA GTTCGCCCTG TCAATATAAC GGCACTTGTT TAGAGAGATC CAAGATAGAG 
TTGTACAAGA GCGACGCGTT AACGTTACCC TCGATATTCA ATCAAGAATT CAGTTACGCG 
AATGCGAGTG GATACGAGTG TCTTTGCGTG CAAGGTGTTA CGGGGAAAAA TTGCGAGGTA 
AATATCAACG AATGTGATAG TAATCCGTGC CAAGCCGGGA CCTGCGTTGA TCGTATCGGT 
GGTTACACCT GCGAATGCGA CGAAGGATAC GAGGGTGATC ATTGTCAACA CGATATCGAT 
GAGTGCAAAA GATATTCGCC CTGCGAGCAT GGTGTGTGTA CAGATGGAAG GGCCGATTAT 
ACTTGTACTT GCGAGCCGGA ATATGGCGGT AAAAATTGTT CGGTGGAGTT GATCGGTTGC 
CAGGGAAACG CTTGTCAAAA TGGCGGTACT TGTTGGCCTT ATCTCGTCGA CGAAACTATA 
CACAAATTCA ATTGCACTTG TCCCAATGGA TATCATGGAG AAATTTGTGA TTATGTAACT 
ACAATGTCTT TGAACGGCAG CTCGTACGTT TTAGTAAACA CTACTCGAGA CGAAGGATAC 
GATATACAAT TTCGTTTTCG AACGACGTTG CCAAATGGAT TATTAGCTAT CGGGAAAGGG 
TCGACGTTTT ACATCCTCGA ACTCGTGAAC GGCAAGTTGA ATTTGCACTC GAGCCTTTTG 
AACAAATGGG AGGGCGTGTT TATCGGAAGC GGTTTGAACG ATTCTACTTG GCAAAAAGTA 
TTCGTCGCGA TCAATGCTAC GCATTTAGTG CTCTCAGCCA ATGAGGAGCA AACTATCTAT 
CCAATCAGTT TGAACGAGGG TTCCAATTCA AATCATACGT CTTTTCCAAT GACTTACGTT 
GGAGGCACCA CCAATTATCT GCGACGATTG ACTCATGGTC CGTCTTTCTT CATCGGCTGT 
ACCGAAGATG TCGTTATTAA TGGAGAATGG GTGTACTCCG GCACCTTGTT GAAAACGGTG 
TACATGGAGG ATATTGAACC AGGATGTCCA CGAGAGGCGC AATGTTCGCC AAATCGGTGT 
AAAAATGGTG GTGACTGTAC AGACAGATGG CGTGATTTTT CCTGCAAATG CGAGAGGCCA 
TATCTTGGCC ACACCTGTCA ATACAACATG ACGGCGGCCA CGTTTGGATA CGAGAATATC 
ACCAACGGAT ACGTGACCGT AAAAGTGTCG GACATGGCCA GGCGAGCAGT TAGATCGATC 
GTCGATATTT CCATGTTCAT ACGTACGAGG CAAGACAGGG GCGACATATT TTATCTTGGA 
ACGGAAAATC CGAATCCGCA GACGGATCTT AAACCTCAAG AGAAAACGTA CATAGCTGCT 
CAACTGGAAG GGGGCGAGTT ACTTGTCAGG ATTCAGTTCA ATGGCACGGA AGCTTACACG 
GTTGGCGGTG TGAAATTGAA CGATGGAAAT AATCACTTGA TACAAGTGAT CAGGAACGTG 
ACGTTGGTGC AAGTGAAAAT TAACGGTACA GAATATTTCC GGAAAACTAT CAGCGCTTCC 
GGCCAGTTGA ACGTAACCGT CTTGTATTTG GGCGGTTTGC CACAAGCATC CAGATACATA 
AGGCAAGTCG ATAATCGTCA GATAGAAGTG TCCCAAGCTC CTCAAGTGAA CTTCAAAGGA 
ATCATTCAAG ATGTTCAGAT ATCCAACGGT GTTGAAACTA TGGTCGTTGA ATTCTTTCCT 
CTAAAGGCAA ACGATATTCC CACTCCGATA CAATTTGGCA ACGTGACATT TGATTATGAG 
AAAATTCTGA AAGGTGTGAT ATCCGACAAC GTATGCGTGA GCAACCCGTG CAATCACAAT 
GGAACTTGTC ACATCACATG GAACGATTTT TGGTGTCAAT GTCCACGAGG ATACACCGGG 
AAAACTTGCC AAGAGATGGA ATTTTGTCAA TTGCAAGACT GTCCCGCTGG ATCGAGATGT 
CAAAATTTAG ACGATGGCTA CGAGTGCATA GCCAATGCCA CATTTAACGG AGTTACCACT 
TCCTTCACCT ATGTTTATAA TCAAGTGGAA GAAAAAAATG TGACCGATTC GACAATCGAC 
ACTATTCAAA TTACGTATCG ATCGAATACC GGTGGCACGT TGATGCATAT CTCGCCTCGT 
ATAGGCGATC AACATTTCAC CGTTTCCGTT TATAAGGACA AGATCACGGT ATCTTGGCGG 
CTAGATTTAC AAAATGAAGG GATATTGAGC TTTGGTAAAA CCGAAACTGA CGGCAATTGG 
ACGTCAATAG TATTACGATT GAACAACAAT TCCATGGAAT GCGGATATGC AAATTCTAAC 
GATGAATATG CATCTCATGT CAGTCCAAAT TTTAGCTTTC CATTATGGTA TGATTTGCTA 
GTTACCGGAA CAGTCACTCT TGGCGGGCTT GGCTCTATTT TATCAAATAA ACACAGTTAC 
ATAACAATCG GTTCCAATAA ACAGAGCTTG GAAAACAAAA GTGGTATTAA CGAGAATTCG 
ATAGATTTCA CTGAACGTAC CACGTTGACC ACCGCTTCTC CCCCTTATAA CATAATGTCA 
GGGGAAGCTT TCAAAGGATG TTTAGGCGAA GTAAGAATTG GTAGTATGCT TTTACATTAC 
TTCACGTATG AAGAAGTATA TCAGAACGCT AATTTTACGC CATTAGAATA TTTAGCGTTA 
CAAGAAAATA ATTCAACGTA TCACGATAGT ATTGGATGCC AGCTTTGTTT CGATTCAGAT 
TGTAAAAATA ATGGATTTTG TCTGGACAAA GCAAACAGTT ACATTTGTGA ATGTCCTGCG 
GGATATACAG AGGACGATTG TTCCTTCAAC ATAGATGAAT GCATTAATAA TAAATGCGAG 
AACGGAGCAA CATGTATCGA TGGAATTGCT AATTACACGT GTATATGTAA CAACGGTTGG 
CAAGGATGGT TATGCGATAG CGACATAAAC GAGTGTGTAA CTCTTAGTCC CTGTCAACAT 
GACGGAGTGT GTGTAAATCT TCCCGGATAT TTCCGTTGCG AATGTCCAGA TCAATTTACC 
GGTGAAAGAT GTGAGATTTT CCGATTAATT ACTTGTGAAA ATCAACCTTG CAAAAATGGT 
GGCAGTTGCA CAGATATTAT AAATCCACAA ACTGGTGATA ATTTCACTTG TACTTGTACG 
ACTGGTTACG AAGGTTCAAT TTGTGATGTT CCTTATTGCA TTGGACAAAA GTGTCAAAAT 
GGCGGCAAAT GTGATTTCTT ATATCAGACA CCTCGATGTA CCTGTTTACC CGGCTATTCT 
GGATTGTATT GTGAAATTAA TATCGATGAT TGCGCACCGG ATGCAGAAGG CAATGTACCG 
TGTAAAAACG ATGGAAAATG CTACGATGGT GTAAACAGTT TCACTTGTGA TTGTAATGAT 
ACCGGCTACA CAGGGCCCGA TTGTTTATTT GATATAGATG AATGTGGAAT TCCAATAACA 
GATTGTGGTC AAGGACGGTG CAAAAATTTA CCAGGAACTT ATCAATGCAT TTGTGACCCG 
GGTTACTGTG GATATAATTG TGGAATGATA GATCCTTGCA AACCGGATTA TTGTCAAAAT 
GGAGGTACAT GTAAATGCGG AGATAATGGT GGTTATAGGT GTGAATGCAC GCCACATTAT 
ACCGGACAAA ATTGTACAGA GACGGAAAAC TTTTTGGGTA GTCAAGCACT CGATATAGCG 
GTAATTGTTG GCCCTATCGT CGGATGTCTG TTTATTATTG CAGCTGGTTC TTTAGTTGCA 
TTATTTATGA TGGCTAGGAA AAAACGAGCT ACTCGAGGAA CGTACAGTCC AAGTGCTCAA 
GAATTCAGCA ATCCGCGTGT AGAAATGGAT AATGTGATGA AACCACCGCC GGAAGAGAGA 
TTAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006569912.2
CDS	360..7208
Translation	MRARYVVAVLTSLLVVTDEAPQDIRHKNHDERPREAYFNGSAFLKLSSFVSVHRHSGLSFRTCEGGRLFTQKYDEDSISLEVTPDGLLFAAIVEQQRYKARLNARLLNNAWHNVNLFFRLGNLTLNAAGHTQVIANATYNAAILTLPDYDMNGSLVVGEGFKGCILQGPGILFNDTMNNGAVFGPCPAENKGCGTSGLNGGVGSSVITTMDFCYLEPCMMHGKCVSRQDRYECHCYARYSGNNCQIDNGPPCLSGPCRNGGTCNEDIKGDFSCACKPGFTGVHCESQLGVRLCEQSPCRNDGVCLAVTETEYKCDCQPGWSGKNCETNINECSPNPCRHGGVCIDGINNYTCICDRTGYEGANCEVDIDECLANPCLNNGVCYDNYGGYICHCPNGFEGQNCELNLNECLSNPCKHGGDCVDDVGSYHCNCPSGYAGRHCELRSLCENAACPSNSICVEDAHGPQCVCNPGYMGNPPNCTINYCANNPCSNGGTCTSNKDGFNCTCPPEWKGTTCLSSASDWCSACYNGGSCLETRFGIMCQCPRFWTGPQCKEPITCRDLPCKQASACHDYPGGYYCTCEAGWTGPECSIDVDECSSDPCRNGGICIDQQNSYYCQCLPGYTGKNCQINVDECLSQPCQNGGTCIDRINGYICNCTKDFMDENCEREYNACAANPCHNNGNCTLIPRSRREFVCECPRGFEGKTCDVNVDDCIDVICPDGQVCVDGIAGYECKCREGYRDPNCTHIVDHCASVSCNSGTCVDLGDQGYECKCKDGFQGKFCDEDVNECNVEGSSLCNNGICVNTDGSYNCFCRPGFSGDHCDIDIDECLCGPCKNNATCIDGINTFECQCPLGYSGKTCDVDVNECESDPCLNGATCVDEIASYMCICSPGFRGLNCEINIDDCEPLPCLNHGQCIDGINNYTCDCSNTGFEGAHCEKNIDDCRSGPCVNGAHCIDDIKNYKCQCYAGYTGKNCEVDINECESSPCQYNGTCLERSKIELYKSDALTLPSIFNQEFSYANASGYECLCVQGVTGKNCEVNINECDSNPCQAGTCVDRIGGYTCECDEGYEGDHCQHDIDECKRYSPCEHGVCTDGRADYTCTCEPEYGGKNCSVELIGCQGNACQNGGTCWPYLVDETIHKFNCTCPNGYHGEICDYVTTMSLNGSSYVLVNTTRDEGYDIQFRFRTTLPNGLLAIGKGSTFYILELVNGKLNLHSSLLNKWEGVFIGSGLNDSTWQKVFVAINATHLVLSANEEQTIYPISLNEGSNSNHTSFPMTYVGGTTNYLRRLTHGPSFFIGCTEDVVINGEWVYSGTLLKTVYMEDIEPGCPREAQCSPNRCKNGGDCTDRWRDFSCKCERPYLGHTCQYNMTAATFGYENITNGYVTVKVSDMARRAVRSIVDISMFIRTRQDRGDIFYLGTENPNPQTDLKPQEKTYIAAQLEGGELLVRIQFNGTEAYTVGGVKLNDGNNHLIQVIRNVTLVQVKINGTEYFRKTISASGQLNVTVLYLGGLPQASRYIRQVDNRQIEVSQAPQVNFKGIIQDVQISNGVETMVVEFFPLKANDIPTPIQFGNVTFDYEKILKGVISDNVCVSNPCNHNGTCHITWNDFWCQCPRGYTGKTCQEMEFCQLQDCPAGSRCQNLDDGYECIANATFNGVTTSFTYVYNQVEEKNVTDSTIDTIQITYRSNTGGTLMHISPRIGDQHFTVSVYKDKITVSWRLDLQNEGILSFGKTETDGNWTSIVLRLNNNSMECGYANSNDEYASHVSPNFSFPLWYDLLVTGTVTLGGLGSILSNKHSYITIGSNKQSLENKSGINENSIDFTERTTLTTASPPYNIMSGEAFKGCLGEVRIGSMLLHYFTYEEVYQNANFTPLEYLALQENNSTYHDSIGCQLCFDSDCKNNGFCLDKANSYICECPAGYTEDDCSFNIDECINNKCENGATCIDGIANYTCICNNGWQGWLCDSDINECVTLSPCQHDGVCVNLPGYFRCECPDQFTGERCEIFRLITCENQPCKNGGSCTDIINPQTGDNFTCTCTTGYEGSICDVPYCIGQKCQNGGKCDFLYQTPRCTCLPGYSGLYCEINIDDCAPDAEGNVPCKNDGKCYDGVNSFTCDCNDTGYTGPDCLFDIDECGIPITDCGQGRCKNLPGTYQCICDPGYCGYNCGMIDPCKPDYCQNGGTCKCGDNGGYRCECTPHYTGQNCTETENFLGSQALDIAVIVGPIVGCLFIIAAGSLVALFMMARKKRATRGTYSPSAQEFSNPRVEMDNVMKPPPEERLI

Target ORF information:

RefSeq Version	XM_006569849.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera protein crumbs (LOC725591), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006569849.2

ORF Insert Sequence:

ATGCGGGCGC GATACGTCGT GGCAGTACTT ACAAGTTTAT TAGTAGTGAC CGACGAAGCG 
CCCCAAGATA TCAGACACAA GAATCACGAT GAACGGCCCA GAGAAGCTTA TTTCAATGGA 
TCGGCATTCC TGAAATTGAG CTCGTTCGTA TCTGTGCACA GGCACAGCGG CTTGAGCTTT 
CGAACTTGCG AGGGCGGCCG GCTCTTCACC CAAAAGTACG ACGAGGACTC GATCAGTCTG 
GAAGTGACCC CGGATGGACT TTTGTTCGCG GCGATTGTCG AGCAGCAGAG ATACAAAGCG 
AGATTGAACG CCAGGCTGTT GAACAACGCC TGGCACAATG TCAATCTATT CTTTAGACTC 
GGAAATCTCA CCTTGAACGC AGCGGGCCAC ACTCAGGTGA TCGCCAACGC GACTTACAAC 
GCTGCCATTC TCACGTTGCC CGATTACGAT ATGAACGGGT CTCTCGTCGT TGGAGAAGGG 
TTCAAAGGAT GTATCCTGCA GGGTCCGGGG ATTCTTTTTA ACGACACGAT GAATAACGGG 
GCAGTTTTTG GCCCCTGCCC CGCGGAAAAT AAAGGATGTG GTACGAGCGG ACTTAACGGT 
GGTGTAGGAT CAAGCGTAAT CACCACCATG GATTTCTGCT ATCTCGAGCC ATGCATGATG 
CATGGTAAAT GCGTGTCACG GCAGGATCGT TACGAGTGTC ACTGTTACGC CCGATATTCA 
GGCAACAACT GTCAAATTGA CAACGGTCCG CCATGCTTGA GTGGGCCATG CCGGAACGGT 
GGTACGTGCA ACGAGGATAT AAAAGGGGAT TTCAGCTGCG CCTGCAAGCC GGGCTTCACC 
GGTGTTCACT GCGAGTCGCA GCTCGGCGTA AGGCTGTGCG AGCAAAGTCC GTGCAGGAAC 
GACGGTGTTT GTTTAGCGGT GACCGAGACC GAATATAAAT GCGATTGCCA GCCGGGATGG 
AGTGGAAAAA ATTGCGAAAC GAATATCAAC GAGTGCTCCC CGAATCCTTG CAGGCACGGC 
GGTGTTTGTA TCGATGGTAT TAATAATTAT ACGTGCATAT GCGACAGGAC TGGATACGAA 
GGTGCGAATT GCGAGGTGGA CATAGACGAA TGCTTGGCTA ATCCTTGCTT GAACAACGGC 
GTGTGCTACG ACAATTACGG CGGTTACATA TGCCACTGCC CGAATGGTTT CGAGGGGCAG 
AACTGCGAGT TGAACTTGAA CGAGTGCTTG TCGAATCCTT GCAAACATGG CGGCGATTGC 
GTGGACGACG TCGGCTCGTA TCACTGCAAT TGCCCGTCCG GATACGCCGG CCGGCACTGC 
GAGCTTAGAA GTTTGTGCGA GAACGCGGCT TGCCCCTCGA ACAGCATCTG CGTGGAGGAC 
GCGCACGGGC CGCAATGCGT GTGTAATCCC GGATACATGG GCAATCCCCC GAATTGCACC 
ATCAATTATT GCGCCAATAA TCCGTGCAGC AACGGGGGCA CTTGCACGAG CAACAAAGAC 
GGATTCAATT GCACTTGCCC ACCGGAATGG AAAGGAACGA CATGCCTGTC ATCCGCCTCG 
GATTGGTGTT CGGCTTGTTA CAATGGAGGC AGTTGTCTCG AGACGCGTTT CGGTATTATG 
TGTCAGTGCC CAAGATTTTG GACGGGACCG CAATGCAAGG AACCGATCAC CTGTCGCGAT 
CTACCTTGCA AACAAGCTTC CGCTTGTCAC GATTATCCCG GCGGTTATTA TTGTACCTGT 
GAGGCAGGAT GGACGGGACC TGAATGTTCC ATCGACGTGG ACGAGTGTTC CAGCGATCCT 
TGCCGTAACG GCGGTATCTG CATTGATCAA CAAAATAGCT ATTATTGTCA ATGTCTTCCA 
GGATACACCG GTAAAAATTG CCAGATCAAC GTGGACGAGT GTTTGTCCCA GCCCTGTCAA 
AATGGCGGCA CTTGCATCGA TCGAATCAAC GGATACATAT GTAACTGCAC GAAAGACTTC 
ATGGATGAAA ATTGCGAGCG GGAGTACAAC GCTTGCGCAG CAAATCCTTG CCACAACAAC 
GGGAACTGCA CGTTGATACC GAGATCTCGG CGTGAATTTG TCTGCGAGTG TCCACGTGGC 
TTCGAAGGGA AAACTTGCGA CGTAAACGTG GACGATTGCA TCGACGTGAT ATGCCCCGAT 
GGGCAAGTTT GCGTCGACGG TATCGCCGGT TACGAGTGCA AATGTCGCGA GGGTTACAGA 
GATCCTAATT GCACTCACAT CGTCGACCAT TGCGCGTCAG TGTCTTGTAA CAGCGGTACG 
TGCGTAGATC TTGGAGATCA AGGATACGAG TGCAAGTGCA AGGATGGTTT CCAAGGAAAA 
TTTTGCGACG AAGATGTGAA CGAATGCAAC GTGGAGGGCA GTTCCTTGTG CAATAACGGT 
ATTTGCGTAA ATACAGACGG CAGCTACAAT TGTTTCTGCA GGCCAGGATT TTCCGGGGAT 
CACTGCGACA TCGACATAGA TGAATGCTTG TGCGGCCCGT GCAAGAATAA TGCCACGTGT 
ATCGACGGTA TCAATACGTT CGAATGCCAG TGCCCGCTTG GTTATTCCGG GAAAACGTGC 
GACGTGGACG TAAACGAGTG CGAAAGTGAT CCCTGTTTAA ACGGTGCGAC TTGCGTCGAT 
GAAATAGCCA GTTACATGTG CATTTGTTCA CCCGGTTTCC GGGGGTTAAA CTGCGAGATA 
AACATCGACG ATTGCGAACC CCTGCCGTGT TTAAACCACG GCCAATGCAT CGACGGTATA 
AACAATTATA CCTGCGACTG TAGCAATACC GGGTTCGAGG GGGCGCATTG CGAGAAAAAT 
ATCGACGATT GCCGGTCCGG CCCATGCGTG AACGGTGCTC ATTGCATAGA CGATATCAAG 
AATTACAAAT GTCAGTGCTA CGCCGGTTAT ACCGGGAAAA ATTGCGAAGT CGATATAAAC 
GAGTGCGAGA GTTCGCCCTG TCAATATAAC GGCACTTGTT TAGAGAGATC CAAGATAGAG 
TTGTACAAGA GCGACGCGTT AACGTTACCC TCGATATTCA ATCAAGAATT CAGTTACGCG 
AATGCGAGTG GATACGAGTG TCTTTGCGTG CAAGGTGTTA CGGGGAAAAA TTGCGAGGTA 
AATATCAACG AATGTGATAG TAATCCGTGC CAAGCCGGGA CCTGCGTTGA TCGTATCGGT 
GGTTACACCT GCGAATGCGA CGAAGGATAC GAGGGTGATC ATTGTCAACA CGATATCGAT 
GAGTGCAAAA GATATTCGCC CTGCGAGCAT GGTGTGTGTA CAGATGGAAG GGCCGATTAT 
ACTTGTACTT GCGAGCCGGA ATATGGCGGT AAAAATTGTT CGGTGGAGTT GATCGGTTGC 
CAGGGAAACG CTTGTCAAAA TGGCGGTACT TGTTGGCCTT ATCTCGTCGA CGAAACTATA 
CACAAATTCA ATTGCACTTG TCCCAATGGA TATCATGGAG AAATTTGTGA TTATGTAACT 
ACAATGTCTT TGAACGGCAG CTCGTACGTT TTAGTAAACA CTACTCGAGA CGAAGGATAC 
GATATACAAT TTCGTTTTCG AACGACGTTG CCAAATGGAT TATTAGCTAT CGGGAAAGGG 
TCGACGTTTT ACATCCTCGA ACTCGTGAAC GGCAAGTTGA ATTTGCACTC GAGCCTTTTG 
AACAAATGGG AGGGCGTGTT TATCGGAAGC GGTTTGAACG ATTCTACTTG GCAAAAAGTA 
TTCGTCGCGA TCAATGCTAC GCATTTAGTG CTCTCAGCCA ATGAGGAGCA AACTATCTAT 
CCAATCAGTT TGAACGAGGG TTCCAATTCA AATCATACGT CTTTTCCAAT GACTTACGTT 
GGAGGCACCA CCAATTATCT GCGACGATTG ACTCATGGTC CGTCTTTCTT CATCGGCTGT 
ACCGAAGATG TCGTTATTAA TGGAGAATGG GTGTACTCCG GCACCTTGTT GAAAACGGTG 
TACATGGAGG ATATTGAACC AGGATGTCCA CGAGAGGCGC AATGTTCGCC AAATCGGTGT 
AAAAATGGTG GTGACTGTAC AGACAGATGG CGTGATTTTT CCTGCAAATG CGAGAGGCCA 
TATCTTGGCC ACACCTGTCA ATACAACATG ACGGCGGCCA CGTTTGGATA CGAGAATATC 
ACCAACGGAT ACGTGACCGT AAAAGTGTCG GACATGGCCA GGCGAGCAGT TAGATCGATC 
GTCGATATTT CCATGTTCAT ACGTACGAGG CAAGACAGGG GCGACATATT TTATCTTGGA 
ACGGAAAATC CGAATCCGCA GACGGATCTT AAACCTCAAG AGAAAACGTA CATAGCTGCT 
CAACTGGAAG GGGGCGAGTT ACTTGTCAGG ATTCAGTTCA ATGGCACGGA AGCTTACACG 
GTTGGCGGTG TGAAATTGAA CGATGGAAAT AATCACTTGA TACAAGTGAT CAGGAACGTG 
ACGTTGGTGC AAGTGAAAAT TAACGGTACA GAATATTTCC GGAAAACTAT CAGCGCTTCC 
GGCCAGTTGA ACGTAACCGT CTTGTATTTG GGCGGTTTGC CACAAGCATC CAGATACATA 
AGGCAAGTCG ATAATCGTCA GATAGAAGTG TCCCAAGCTC CTCAAGTGAA CTTCAAAGGA 
ATCATTCAAG ATGTTCAGAT ATCCAACGGT GTTGAAACTA TGGTCGTTGA ATTCTTTCCT 
CTAAAGGCAA ACGATATTCC CACTCCGATA CAATTTGGCA ACGTGACATT TGATTATGAG 
AAAATTCTGA AAGGTGTGAT ATCCGACAAC GTATGCGTGA GCAACCCGTG CAATCACAAT 
GGAACTTGTC ACATCACATG GAACGATTTT TGGTGTCAAT GTCCACGAGG ATACACCGGG 
AAAACTTGCC AAGAGATGGA ATTTTGTCAA TTGCAAGACT GTCCCGCTGG ATCGAGATGT 
CAAAATTTAG ACGATGGCTA CGAGTGCATA GCCAATGCCA CATTTAACGG AGTTACCACT 
TCCTTCACCT ATGTTTATAA TCAAGTGGAA GAAAAAAATG TGACCGATTC GACAATCGAC 
ACTATTCAAA TTACGTATCG ATCGAATACC GGTGGCACGT TGATGCATAT CTCGCCTCGT 
ATAGGCGATC AACATTTCAC CGTTTCCGTT TATAAGGACA AGATCACGGT ATCTTGGCGG 
CTAGATTTAC AAAATGAAGG GATATTGAGC TTTGGTAAAA CCGAAACTGA CGGCAATTGG 
ACGTCAATAG TATTACGATT GAACAACAAT TCCATGGAAT GCGGATATGC AAATTCTAAC 
GATGAATATG CATCTCATGT CAGTCCAAAT TTTAGCTTTC CATTATGGTA TGATTTGCTA 
GTTACCGGAA CAGTCACTCT TGGCGGGCTT GGCTCTATTT TATCAAATAA ACACAGTTAC 
ATAACAATCG GTTCCAATAA ACAGAGCTTG GAAAACAAAA GTGGTATTAA CGAGAATTCG 
ATAGATTTCA CTGAACGTAC CACGTTGACC ACCGCTTCTC CCCCTTATAA CATAATGTCA 
GGGGAAGCTT TCAAAGGATG TTTAGGCGAA GTAAGAATTG GTAGTATGCT TTTACATTAC 
TTCACGTATG AAGAAGTATA TCAGAACGCT AATTTTACGC CATTAGAATA TTTAGCGTTA 
CAAGAAAATA ATTCAACGTA TCACGATAGT ATTGGATGCC AGCTTTGTTT CGATTCAGAT 
TGTAAAAATA ATGGATTTTG TCTGGACAAA GCAAACAGTT ACATTTGTGA ATGTCCTGCG 
GGATATACAG AGGACGATTG TTCCTTCAAC ATAGATGAAT GCATTAATAA TAAATGCGAG 
AACGGAGCAA CATGTATCGA TGGAATTGCT AATTACACGT GTATATGTAA CAACGGTTGG 
CAAGGATGGT TATGCGATAG CGACATAAAC GAGTGTGTAA CTCTTAGTCC CTGTCAACAT 
GACGGAGTGT GTGTAAATCT TCCCGGATAT TTCCGTTGCG AATGTCCAGA TCAATTTACC 
GGTGAAAGAT GTGAGATTTT CCGATTAATT ACTTGTGAAA ATCAACCTTG CAAAAATGGT 
GGCAGTTGCA CAGATATTAT AAATCCACAA ACTGGTGATA ATTTCACTTG TACTTGTACG 
ACTGGTTACG AAGGTTCAAT TTGTGATGTT CCTTATTGCA TTGGACAAAA GTGTCAAAAT 
GGCGGCAAAT GTGATTTCTT ATATCAGACA CCTCGATGTA CCTGTTTACC CGGCTATTCT 
GGATTGTATT GTGAAATTAA TATCGATGAT TGCGCACCGG ATGCAGAAGG CAATGTACCG 
TGTAAAAACG ATGGAAAATG CTACGATGGT GTAAACAGTT TCACTTGTGA TTGTAATGAT 
ACCGGCTACA CAGGGCCCGA TTGTTTATTT GATATAGATG AATGTGGAAT TCCAATAACA 
GATTGTGGTC AAGGACGGTG CAAAAATTTA CCAGGAACTT ATCAATGCAT TTGTGACCCG 
GGTTACTGTG GATATAATTG TGGAATGATA GATCCTTGCA AACCGGATTA TTGTCAAAAT 
GGAGGTACAT GTAAATGCGG AGATAATGGT GGTTATAGGT GTGAATGCAC GCCACATTAT 
ACCGGACAAA ATTGTACAGA GACGGAAAAC TTTTTGGGTA GTCAAGCACT CGATATAGCG 
GTAATTGTTG GCCCTATCGT CGGATGTCTG TTTATTATTG CAGCTGGTTC TTTAGTTGCA 
TTATTTATGA TGGCTAGGAA AAAACGAGCT ACTCGAGGAA CGTACAGTCC AAGTGCTCAA 
GAATTCAGCA ATCCGCGTGT AGAAATGGAT AATGTGATGA AACCACCGCC GGAAGAGAGA 
TTAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC725591 ( XM_026439371.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026295156.1 Apis mellifera protein crumbs (LOC725591), transcript variant X1, mRNA.

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CloneID	OHd29285
Clone ID Related Accession (Same CDS sequence)	XM_026439371.1
Accession Version	XM_026439371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	protein crumbs isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037640.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCGC GATACGTCGT GGCAGTACTT ACAAGTTTAT TAGTAGTGAC CGACGAAGCG 
CCCCAAGATA TCAGACACAA GAATCACGAT GAACGGCCCA GAGAAGCTTA TTTCAATGGA 
TCGGCATTCC TGAAATTGAG CTCGTTCGTA TCTGTGCACA GGCACAGCGG CTTGAGCTTT 
CGAACTTGCG AGGGCGGCCG GCTCTTCACC CAAAAGTACG ACGAGGACTC GATCAGTCTG 
GAAGTGACCC CGGACGGACT TTTGTTCGCG GCGATTGTCG AGCAGCAGAG ATACAAAGCG 
AGATTGAACG CCAGGCTGTT GAACAACGCC TGGCACAATG TCAATCTATT CTTTAGACTC 
GGAAATCTCA CCTTGAACGC AGCGGGCCAC ACTCAGGTGA TCGCCAACGC GACTTACAAC 
GCTGCCATTC TCACGTTGCC CGATTACGAT ATGAACGGGT CTCTCGTCGT TGGAGAAGGG 
TTCAAAGGAT GTATCCTGCA GGGTCCGGGG ATTCTTTTTA ACGACACGAT GAATAACGGG 
GCAGTTTTTG GCCCCTGCCC CGCGGAAAAT AAAGGATGTG GTACGAGCGG ACTTAACGGT 
GGTGTAGGAT CAAGCGTAAT CACCACCATG GATTTCTGCT ATCTCGAGCC ATGCATGATG 
CATGGTAAAT GCGTGTCACG GCAGGATCGT TACGAGTGTC ACTGTTACGC CCGATATTCA 
GGCAACAACT GTCAAATTGA CAACGGTCCG CCATGCTTGA GTGGGCCATG CCGGAACGGT 
GGTACGTGCA ACGAGGATAT AAAAGGGGAT TTCAGCTGCG CCTGCAAGCC GGGCTTCACC 
GGTGTTCACT GCGAGTCGCA GCTCGGCGTA AGGCTGTGCG AGCAAAGTCC GTGCAGGAAC 
GACGGTGTTT GTTTAGCGGT GACCGAGACC GAATATAAAT GCGATTGCCA GCCGGGATGG 
AGTGGAAAAA ATTGCGAAAC GAATATCAAC GAGTGCTCCC CGAATCCTTG CAGGCACGGC 
GGTGTTTGTA TCGATGGTAT TAATAATTAT ACGTGCATAT GCGACAGGAC TGGATACGAA 
GGTGCGAATT GCGAGGTGGA CATAGACGAA TGCTTGGCTA ATCCTTGCTT GAACAACGGC 
GTGTGCTACG ACAATTACGG CGGTTACATA TGCCACTGCC CGAATGGTTT CGAGGGGCAG 
AACTGCGAGT TGAACTTGAA CGAGTGCTTG TCGAATCCTT GCAAACATGG CGGCGATTGC 
GTGGACGACG TCGGCTCGTA TCACTGCAAT TGCCCGTCCG GATACGCCGG CCGGCACTGC 
GAGCTTAGAA GTTTGTGCGA GAACGCGGCT TGCCCCTCGA ACAGCATCTG CGTGGAGGAC 
GCGCACGGGC CGCAATGCGT GTGTAATCCC GGATACATGG GCAATCCCCC GAATTGCACC 
ATCAATTATT GCGCCAATAA TCCGTGCAGC AACGGGGGCA CTTGCACGAG CAACAAAGAC 
GGATTCAATT GCACTTGCCC ACCGGAATGG AAAGGAACGA CATGCCTGTC ATCCGCCTCG 
GATTGGTGTT CGGCTTGTTA CAATGGAGGC AGTTGTCTCG AGACGCGTTT CGGTATTATG 
TGTCAGTGCC CAAGATTTTG GACGGGACCG CAATGCAAGG AACCGATCAC CTGTCGCGAT 
CTACCTTGCA AACAAGCTTC CGCTTGTCAC GATTATCCCG GCGGTTATTA TTGTACCTGT 
GAGGCAGGAT GGACGGGACC TGAATGTTCC ATCGACGTGG ACGAGTGTTC CAGCGATCCT 
TGCCGTAACG GCGGTATCTG CATTGATCAA CAAAATAGCT ATTATTGTCA ATGTCTTCCA 
GGATACACCG GTAAAAATTG CCAGATCAAC GTGGACGAGT GTTTGTCCCA GCCCTGTCAA 
AATGGCGGCA CTTGCATCGA TCGAATCAAC GGATACATAT GTAACTGCAC GAAAGACTTC 
ATGGATGAAA ATTGCGAGCG GGAGTACAAC GCTTGCGCAG CAAATCCTTG CCACAACAAC 
GGGAACTGCA CGTTGATACC GAGATCTCGG CGTGAATTTG TCTGCGAGTG TCCACGTGGC 
TTCGAAGGGA AAACTTGCGA CGTAAACGTG GACGATTGTA TCGACGTGAT ATGCCCCGAT 
GGGCAGGTTT GCGTCGACGG TATCGCCGGT TACGAGTGCA AATGTCGCGA GGGTTACAGA 
GATCCTAATT GCACTTACAT CGTCGACCAT TGCGCGTCAG TGTCTTGTAA CAGCGGTACG 
TGCGTAGATC TTGGAGATCA AGGATACGAG TGCAAGTGCA AGGATGGTTT CCAAGGAAAA 
TTTTGCGACG AAGATGTGAA CGAATGCAAC GTGGAGGGCA GTTCCTTGTG CAATAACGGT 
ATTTGCGTAA ATACAGACGG CAGCTACAAT TGTTTCTGCA GGCCAGGATT TTCCGGGGAT 
CACTGCGACA TCGACATAGA TGAATGCTTG TGCGGCCCGT GCAAGAATAA TGCCACGTGT 
ATCGACGGTA TCAATACGTT CGAATGCCAG TGCCCGCTTG GTTATTCCGG GAAAACGTGC 
GACGTGGACG TAAACGAGTG CGAAAGTGAT CCCTGTTTAA ACGGTGCGAC TTGCGTCGAT 
GAAATAGCCA GTTACATGTG CATTTGTTCA CCCGGTTTCC GGGGGTTAAA CTGCGAGATA 
AACATCGACG ATTGCGAACC CCTGCCGTGT TTAAACCACG GCCAATGCAT CGACGGTATA 
AACAATTATA CCTGCGACTG TAGCAATACC GGGTTCGAGG GGGCGCATTG CGAGAAAAAT 
ATCGACGATT GCCGGTCCGG CCCATGCGTG AACGGTGCTC ATTGCATAGA CGATATCAAG 
AATTACAAAT GTCAGTGCTA CGCCGGTTAT ACCGGGAAAA ATTGCGAAGT CGATATAAAC 
GAGTGCGAGA GTTCGCCCTG TCAATATAAC GGCACTTGTT TAGAGAGATC CAAGATAGAG 
TTGTACAAGA GCGACGCGTT AACGTTACCC TCGATATTCA ATCAAGAATT CAGTTACGCG 
AATGCGAGTG GATACGAGTG TCTTTGCGTG CAAGGTGTTA CGGGGAAAAA TTGCGAGGTA 
AATATCAACG AATGTGATAG TAATCCGTGC CAAGCCGGGA CCTGCGTTGA TCGTATCGGT 
GGTTACACCT GCGAATGCGA CGAAGGATAC GAGGGTGATC ATTGTCAACA CGATATCGAT 
GAGTGCAAAA GATATTCGCC CTGCGAGCAT GGTGTGTGTA CAGATGGAAG GGCCGATTAT 
ACTTGTACTT GCGAGCCGGA ATATGGCGGT AAAAATTGTT CGGTGGAGTT GATCGGTTGC 
CAGGGAAACG CTTGTCAAAA TGGCGGTACT TGTTGGCCTT ATCTCGTCGA CGAAACTATA 
CACAAATTCA ATTGCACTTG TCCCAATGGA TATCATGGAG AAATTTGTGA TTATGTAACT 
ACAATGTCTT TGAACGGCAG CTCGTACGTT TTAGTAAACA CTACTCGAGA CGAAGGATAC 
GATATACAAT TTCGTTTTCG AACGACGTTG CCAAATGGAT TATTAGCTAT CGGGAAAGGG 
TCGACGTTTT ACATCCTCGA ACTCGTGAAC GGCAAGTTGA ATTTGCACTC GAGCCTTTTG 
AACAAATGGG AGGGCGTGTT TATCGGAAGC GGTTTGAACG ATTCTACTTG GCAAAAAGTA 
TTCGTCGCGA TCAATGCTAC GCATTTAGTG CTCTCAGCCA ATGAGGAGCA AACTATCTAT 
CCAATCAGTT TGAACGAGGG TTCCAATTCA AATCATACGT CTTTTCCAAT GACTTACGTT 
GGAGGCACCA CCAATTATCT GCGACGATTG ACTCATGGTC CGTCTTTCTT CATCGGCTGT 
ACCGAAGATG TCGTTATTAA TGGAGAATGG GTGTACTCCG GCACCTTGTT GAAAACGGTG 
TACATGGAGG ATATTGAACC AGGATGTCCA CGAGAGGCGC AATGTTCGCC AAATCGGTGT 
AAAAATGGTG GTGACTGTAC AGACAGATGG CGTGATTTTT CCTGCAAATG CGAGAGGCCA 
TATCTTGGCC ACACCTGTCA ATACAACATG ACGGCGGCCA CGTTTGGATA CGAGAATATC 
ACCAACGGAT ACGTGACCGT AAAAGTGTCG GACATGGCCA GGCGAGCAGT TAGATCGATC 
GTCGATATTT CCATGTTCAT ACGTACGAGG CAAGACAGGG GCGACATATT TTATCTTGGA 
ACGGAAAATC CGAATCCGCA GACGGATCTT AAACCTCAAG AGAAAACGTA CATAGCTGCT 
CAACTGGAAG GGGGCGAGTT ACTTGTCAGG ATTCAGTTCA ATGGCACGGA AGCTTACACG 
GTTGGCGGTG TGAAATTGAA CGATGGAAAT AATCACTTGA TACAAGTGAT CAGGAACGTG 
ACGTTGGTGC AAGTGAAAAT TAACGGTACA GAATATTTCC GGAAAACTAT CAGCGCTTCC 
GGCCAGTTGA ACGTAACCGT CTTGTATTTG GGCGGTTTGC CACAAGCATC CAGATACATA 
AGGCAAGTCG ATAATCGTCA GATAGAAGTG TCCCAAGCTC CTCAAGTGAA CTTCAAAGGA 
ATCATTCAAG ATGTTCAGAT ATCCAACGGT GTTGAAACTA TGGTCGTTGA ATTCTTTCCT 
CTAAAGGCAA ACGATATTCC CACTCCGATA CAATTTGGCA ACGTGACATT TGATTATGAG 
AAAATTCTGA AAGGTGTGAT ATCCGACAAC GTATGCGTGA GCAACCCGTG CAATCACAAT 
GGAACTTGTC ACATCACATG GAACGATTTT TGGTGTCAAT GTCCACGAGG ATACACCGGG 
AAAACTTGCC AAGAGATGGA ATTTTGTCAA TTGCAAGACT GTCCCGCTGG ATCGAGATGT 
CAAAATTTAG ACGATGGCTA CGAGTGCATA GCCAATGCCA CATTTAACGG AGTTACCACT 
TCCTTCACCT ATGTTTATAA TCAAGTGGAA GAAAAAAATG TGACCGATTC GACAATCGAC 
ACTATTCAAA TTACGTATCG ATCGAATACC GGTGGCACGT TGATGCATAT CTCGCCTCGT 
ATAGGCGATC AACATTTCAC CGTTTCCGTT TATAAGGACA AGATCACGGT ATCTTGGCGG 
CTAGATTTAC AAAATGAAGG GATATTGAGC TTTGGTAAAA CCGAAACTGA CGGCAATTGG 
ACGTCAATAG TATTACGATT GAACAACAAT TCCATGGAAT GCGGATATGC AAATTCTAAC 
GATGAATATG CATCTCATGT CAGTCCAAAT TTTAGCTTTC CATTATGGTA TGATTTGCTA 
GTTACCGGAA CAGTCACTCT TGGCGGGCTT GGCTCTATTT TATCAAATAA ACACAGTTAC 
ATAACAATCG GTTCCAATAA ACAGAGCTTG GAAAACAAAA GTGGTATTAA CGAGAATTCG 
ATAGATTTCA CTGAACGTAC CACGTTGACC ACCGCTTCTC CCCCTTATAA CATAATGTCA 
GGGGAAGCTT TCAAAGGATG TTTAGGCGAA GTAAGAATTG GTAGTATGCT TTTACATTAC 
TTCACGTATG AAGAAGTATA TCAGAACGCT AATTTTACGC CATTAGAATA TTTAGCGTTA 
CAAGAAAATA ATTCAACGTA TCACGATAGT ATTGGATGCC AGCTTTGTTT CGATTCAGAT 
TGTAAAAATA ATGGATTTTG TCTGGACAAA GCAAACAGTT ACATTTGTGA ATGTCCTGCG 
GGATATACAG AGGACGATTG TTCCTTCAAC ATAGATGAAT GCATTAATAA TAAATGCGAG 
AACGGAGCAA CATGTATCGA TGGAATTGCT AATTACACGT GTATATGTAA CAACGGTTGG 
CAAGGATGGT TATGCGATAG CGACATAAAC GAGTGTGTAA CTCTTAGTCC CTGTCAACAT 
GACGGAGTGT GTGTAAATCT TCCCGGATAT TTCCGTTGCG AATGTCCAGA TCAATTTACC 
GGTGAAAGAT GTGAGATTTT CCGATTAATT ACTTGTGAAA ATCAACCTTG CAAAAATGGT 
GGCAGTTGCA CAGATATTAT AAATCCACAA ACTGGTGATA ATTTCACTTG TACTTGTACG 
ACTGGTTACG AAGGTTCAAT TTGTGATGTT CCTTATTGCA TTGGACAAAA GTGTCAAAAT 
GGCGGCAAAT GTGATTTCTT ATATCAGACA CCTCGATGTA CCTGTTTACC CGGCTATTCT 
GGATTGTATT GTGAAATTAA TATCGATGAT TGCGCACCGG ATGCAGAAGG CAATGTACCG 
TGTAAAAACG ATGGAAAATG CTACGATGGT GTAAACAGTT TCACTTGTGA TTGTAATGAT 
ACCGGCTACA CAGGGCCCGA TTGTTTATTT GATATAGATG AATGTGGAAT TCCAATAACA 
GATTGTGGTC AAGGACGGTG CAAAAATTTA CCAGGAACTT ATCAATGCAT TTGTGACCCG 
GGTTACTGTG GATATAATTG TGGAATGATA GATCCTTGCA AACCGGATTA TTGTCAAAAT 
GGAGGTACAT GTAAATGCGG AGATAATGGT GGTTATAGGT GTGAATGCAC GCCACATTAT 
ACCGGACAAA ATTGTACAGA GACGGAAAAC TTTTTGGGTA GTCAAGCACT CGATATAGCG 
GTAATTGTTG GCCCTATCGT CGGATGTCTG TTTATTATTG CAGCTGGTTC TTTAGTTGCA 
TTATTTATGA TGGCTAGGAA AAAACGAGCT ACTCGAGGAA CGTACAGTCC AAGTGCTCAA 
GAATTCAGCA ATCCGCGTGT AGAAATGGAT AATGTGATGA AACCACCGCC GGAAGAGAGA 
TTAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026295156.1
CDS	331..7179
Translation	MRARYVVAVLTSLLVVTDEAPQDIRHKNHDERPREAYFNGSAFLKLSSFVSVHRHSGLSFRTCEGGRLFTQKYDEDSISLEVTPDGLLFAAIVEQQRYKARLNARLLNNAWHNVNLFFRLGNLTLNAAGHTQVIANATYNAAILTLPDYDMNGSLVVGEGFKGCILQGPGILFNDTMNNGAVFGPCPAENKGCGTSGLNGGVGSSVITTMDFCYLEPCMMHGKCVSRQDRYECHCYARYSGNNCQIDNGPPCLSGPCRNGGTCNEDIKGDFSCACKPGFTGVHCESQLGVRLCEQSPCRNDGVCLAVTETEYKCDCQPGWSGKNCETNINECSPNPCRHGGVCIDGINNYTCICDRTGYEGANCEVDIDECLANPCLNNGVCYDNYGGYICHCPNGFEGQNCELNLNECLSNPCKHGGDCVDDVGSYHCNCPSGYAGRHCELRSLCENAACPSNSICVEDAHGPQCVCNPGYMGNPPNCTINYCANNPCSNGGTCTSNKDGFNCTCPPEWKGTTCLSSASDWCSACYNGGSCLETRFGIMCQCPRFWTGPQCKEPITCRDLPCKQASACHDYPGGYYCTCEAGWTGPECSIDVDECSSDPCRNGGICIDQQNSYYCQCLPGYTGKNCQINVDECLSQPCQNGGTCIDRINGYICNCTKDFMDENCEREYNACAANPCHNNGNCTLIPRSRREFVCECPRGFEGKTCDVNVDDCIDVICPDGQVCVDGIAGYECKCREGYRDPNCTYIVDHCASVSCNSGTCVDLGDQGYECKCKDGFQGKFCDEDVNECNVEGSSLCNNGICVNTDGSYNCFCRPGFSGDHCDIDIDECLCGPCKNNATCIDGINTFECQCPLGYSGKTCDVDVNECESDPCLNGATCVDEIASYMCICSPGFRGLNCEINIDDCEPLPCLNHGQCIDGINNYTCDCSNTGFEGAHCEKNIDDCRSGPCVNGAHCIDDIKNYKCQCYAGYTGKNCEVDINECESSPCQYNGTCLERSKIELYKSDALTLPSIFNQEFSYANASGYECLCVQGVTGKNCEVNINECDSNPCQAGTCVDRIGGYTCECDEGYEGDHCQHDIDECKRYSPCEHGVCTDGRADYTCTCEPEYGGKNCSVELIGCQGNACQNGGTCWPYLVDETIHKFNCTCPNGYHGEICDYVTTMSLNGSSYVLVNTTRDEGYDIQFRFRTTLPNGLLAIGKGSTFYILELVNGKLNLHSSLLNKWEGVFIGSGLNDSTWQKVFVAINATHLVLSANEEQTIYPISLNEGSNSNHTSFPMTYVGGTTNYLRRLTHGPSFFIGCTEDVVINGEWVYSGTLLKTVYMEDIEPGCPREAQCSPNRCKNGGDCTDRWRDFSCKCERPYLGHTCQYNMTAATFGYENITNGYVTVKVSDMARRAVRSIVDISMFIRTRQDRGDIFYLGTENPNPQTDLKPQEKTYIAAQLEGGELLVRIQFNGTEAYTVGGVKLNDGNNHLIQVIRNVTLVQVKINGTEYFRKTISASGQLNVTVLYLGGLPQASRYIRQVDNRQIEVSQAPQVNFKGIIQDVQISNGVETMVVEFFPLKANDIPTPIQFGNVTFDYEKILKGVISDNVCVSNPCNHNGTCHITWNDFWCQCPRGYTGKTCQEMEFCQLQDCPAGSRCQNLDDGYECIANATFNGVTTSFTYVYNQVEEKNVTDSTIDTIQITYRSNTGGTLMHISPRIGDQHFTVSVYKDKITVSWRLDLQNEGILSFGKTETDGNWTSIVLRLNNNSMECGYANSNDEYASHVSPNFSFPLWYDLLVTGTVTLGGLGSILSNKHSYITIGSNKQSLENKSGINENSIDFTERTTLTTASPPYNIMSGEAFKGCLGEVRIGSMLLHYFTYEEVYQNANFTPLEYLALQENNSTYHDSIGCQLCFDSDCKNNGFCLDKANSYICECPAGYTEDDCSFNIDECINNKCENGATCIDGIANYTCICNNGWQGWLCDSDINECVTLSPCQHDGVCVNLPGYFRCECPDQFTGERCEIFRLITCENQPCKNGGSCTDIINPQTGDNFTCTCTTGYEGSICDVPYCIGQKCQNGGKCDFLYQTPRCTCLPGYSGLYCEINIDDCAPDAEGNVPCKNDGKCYDGVNSFTCDCNDTGYTGPDCLFDIDECGIPITDCGQGRCKNLPGTYQCICDPGYCGYNCGMIDPCKPDYCQNGGTCKCGDNGGYRCECTPHYTGQNCTETENFLGSQALDIAVIVGPIVGCLFIIAAGSLVALFMMARKKRATRGTYSPSAQEFSNPRVEMDNVMKPPPEERLI

Target ORF information:

RefSeq Version	XM_026439371.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera protein crumbs (LOC725591), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026439371.1

ORF Insert Sequence:

ATGCGGGCGC GATACGTCGT GGCAGTACTT ACAAGTTTAT TAGTAGTGAC CGACGAAGCG 
CCCCAAGATA TCAGACACAA GAATCACGAT GAACGGCCCA GAGAAGCTTA TTTCAATGGA 
TCGGCATTCC TGAAATTGAG CTCGTTCGTA TCTGTGCACA GGCACAGCGG CTTGAGCTTT 
CGAACTTGCG AGGGCGGCCG GCTCTTCACC CAAAAGTACG ACGAGGACTC GATCAGTCTG 
GAAGTGACCC CGGACGGACT TTTGTTCGCG GCGATTGTCG AGCAGCAGAG ATACAAAGCG 
AGATTGAACG CCAGGCTGTT GAACAACGCC TGGCACAATG TCAATCTATT CTTTAGACTC 
GGAAATCTCA CCTTGAACGC AGCGGGCCAC ACTCAGGTGA TCGCCAACGC GACTTACAAC 
GCTGCCATTC TCACGTTGCC CGATTACGAT ATGAACGGGT CTCTCGTCGT TGGAGAAGGG 
TTCAAAGGAT GTATCCTGCA GGGTCCGGGG ATTCTTTTTA ACGACACGAT GAATAACGGG 
GCAGTTTTTG GCCCCTGCCC CGCGGAAAAT AAAGGATGTG GTACGAGCGG ACTTAACGGT 
GGTGTAGGAT CAAGCGTAAT CACCACCATG GATTTCTGCT ATCTCGAGCC ATGCATGATG 
CATGGTAAAT GCGTGTCACG GCAGGATCGT TACGAGTGTC ACTGTTACGC CCGATATTCA 
GGCAACAACT GTCAAATTGA CAACGGTCCG CCATGCTTGA GTGGGCCATG CCGGAACGGT 
GGTACGTGCA ACGAGGATAT AAAAGGGGAT TTCAGCTGCG CCTGCAAGCC GGGCTTCACC 
GGTGTTCACT GCGAGTCGCA GCTCGGCGTA AGGCTGTGCG AGCAAAGTCC GTGCAGGAAC 
GACGGTGTTT GTTTAGCGGT GACCGAGACC GAATATAAAT GCGATTGCCA GCCGGGATGG 
AGTGGAAAAA ATTGCGAAAC GAATATCAAC GAGTGCTCCC CGAATCCTTG CAGGCACGGC 
GGTGTTTGTA TCGATGGTAT TAATAATTAT ACGTGCATAT GCGACAGGAC TGGATACGAA 
GGTGCGAATT GCGAGGTGGA CATAGACGAA TGCTTGGCTA ATCCTTGCTT GAACAACGGC 
GTGTGCTACG ACAATTACGG CGGTTACATA TGCCACTGCC CGAATGGTTT CGAGGGGCAG 
AACTGCGAGT TGAACTTGAA CGAGTGCTTG TCGAATCCTT GCAAACATGG CGGCGATTGC 
GTGGACGACG TCGGCTCGTA TCACTGCAAT TGCCCGTCCG GATACGCCGG CCGGCACTGC 
GAGCTTAGAA GTTTGTGCGA GAACGCGGCT TGCCCCTCGA ACAGCATCTG CGTGGAGGAC 
GCGCACGGGC CGCAATGCGT GTGTAATCCC GGATACATGG GCAATCCCCC GAATTGCACC 
ATCAATTATT GCGCCAATAA TCCGTGCAGC AACGGGGGCA CTTGCACGAG CAACAAAGAC 
GGATTCAATT GCACTTGCCC ACCGGAATGG AAAGGAACGA CATGCCTGTC ATCCGCCTCG 
GATTGGTGTT CGGCTTGTTA CAATGGAGGC AGTTGTCTCG AGACGCGTTT CGGTATTATG 
TGTCAGTGCC CAAGATTTTG GACGGGACCG CAATGCAAGG AACCGATCAC CTGTCGCGAT 
CTACCTTGCA AACAAGCTTC CGCTTGTCAC GATTATCCCG GCGGTTATTA TTGTACCTGT 
GAGGCAGGAT GGACGGGACC TGAATGTTCC ATCGACGTGG ACGAGTGTTC CAGCGATCCT 
TGCCGTAACG GCGGTATCTG CATTGATCAA CAAAATAGCT ATTATTGTCA ATGTCTTCCA 
GGATACACCG GTAAAAATTG CCAGATCAAC GTGGACGAGT GTTTGTCCCA GCCCTGTCAA 
AATGGCGGCA CTTGCATCGA TCGAATCAAC GGATACATAT GTAACTGCAC GAAAGACTTC 
ATGGATGAAA ATTGCGAGCG GGAGTACAAC GCTTGCGCAG CAAATCCTTG CCACAACAAC 
GGGAACTGCA CGTTGATACC GAGATCTCGG CGTGAATTTG TCTGCGAGTG TCCACGTGGC 
TTCGAAGGGA AAACTTGCGA CGTAAACGTG GACGATTGTA TCGACGTGAT ATGCCCCGAT 
GGGCAGGTTT GCGTCGACGG TATCGCCGGT TACGAGTGCA AATGTCGCGA GGGTTACAGA 
GATCCTAATT GCACTTACAT CGTCGACCAT TGCGCGTCAG TGTCTTGTAA CAGCGGTACG 
TGCGTAGATC TTGGAGATCA AGGATACGAG TGCAAGTGCA AGGATGGTTT CCAAGGAAAA 
TTTTGCGACG AAGATGTGAA CGAATGCAAC GTGGAGGGCA GTTCCTTGTG CAATAACGGT 
ATTTGCGTAA ATACAGACGG CAGCTACAAT TGTTTCTGCA GGCCAGGATT TTCCGGGGAT 
CACTGCGACA TCGACATAGA TGAATGCTTG TGCGGCCCGT GCAAGAATAA TGCCACGTGT 
ATCGACGGTA TCAATACGTT CGAATGCCAG TGCCCGCTTG GTTATTCCGG GAAAACGTGC 
GACGTGGACG TAAACGAGTG CGAAAGTGAT CCCTGTTTAA ACGGTGCGAC TTGCGTCGAT 
GAAATAGCCA GTTACATGTG CATTTGTTCA CCCGGTTTCC GGGGGTTAAA CTGCGAGATA 
AACATCGACG ATTGCGAACC CCTGCCGTGT TTAAACCACG GCCAATGCAT CGACGGTATA 
AACAATTATA CCTGCGACTG TAGCAATACC GGGTTCGAGG GGGCGCATTG CGAGAAAAAT 
ATCGACGATT GCCGGTCCGG CCCATGCGTG AACGGTGCTC ATTGCATAGA CGATATCAAG 
AATTACAAAT GTCAGTGCTA CGCCGGTTAT ACCGGGAAAA ATTGCGAAGT CGATATAAAC 
GAGTGCGAGA GTTCGCCCTG TCAATATAAC GGCACTTGTT TAGAGAGATC CAAGATAGAG 
TTGTACAAGA GCGACGCGTT AACGTTACCC TCGATATTCA ATCAAGAATT CAGTTACGCG 
AATGCGAGTG GATACGAGTG TCTTTGCGTG CAAGGTGTTA CGGGGAAAAA TTGCGAGGTA 
AATATCAACG AATGTGATAG TAATCCGTGC CAAGCCGGGA CCTGCGTTGA TCGTATCGGT 
GGTTACACCT GCGAATGCGA CGAAGGATAC GAGGGTGATC ATTGTCAACA CGATATCGAT 
GAGTGCAAAA GATATTCGCC CTGCGAGCAT GGTGTGTGTA CAGATGGAAG GGCCGATTAT 
ACTTGTACTT GCGAGCCGGA ATATGGCGGT AAAAATTGTT CGGTGGAGTT GATCGGTTGC 
CAGGGAAACG CTTGTCAAAA TGGCGGTACT TGTTGGCCTT ATCTCGTCGA CGAAACTATA 
CACAAATTCA ATTGCACTTG TCCCAATGGA TATCATGGAG AAATTTGTGA TTATGTAACT 
ACAATGTCTT TGAACGGCAG CTCGTACGTT TTAGTAAACA CTACTCGAGA CGAAGGATAC 
GATATACAAT TTCGTTTTCG AACGACGTTG CCAAATGGAT TATTAGCTAT CGGGAAAGGG 
TCGACGTTTT ACATCCTCGA ACTCGTGAAC GGCAAGTTGA ATTTGCACTC GAGCCTTTTG 
AACAAATGGG AGGGCGTGTT TATCGGAAGC GGTTTGAACG ATTCTACTTG GCAAAAAGTA 
TTCGTCGCGA TCAATGCTAC GCATTTAGTG CTCTCAGCCA ATGAGGAGCA AACTATCTAT 
CCAATCAGTT TGAACGAGGG TTCCAATTCA AATCATACGT CTTTTCCAAT GACTTACGTT 
GGAGGCACCA CCAATTATCT GCGACGATTG ACTCATGGTC CGTCTTTCTT CATCGGCTGT 
ACCGAAGATG TCGTTATTAA TGGAGAATGG GTGTACTCCG GCACCTTGTT GAAAACGGTG 
TACATGGAGG ATATTGAACC AGGATGTCCA CGAGAGGCGC AATGTTCGCC AAATCGGTGT 
AAAAATGGTG GTGACTGTAC AGACAGATGG CGTGATTTTT CCTGCAAATG CGAGAGGCCA 
TATCTTGGCC ACACCTGTCA ATACAACATG ACGGCGGCCA CGTTTGGATA CGAGAATATC 
ACCAACGGAT ACGTGACCGT AAAAGTGTCG GACATGGCCA GGCGAGCAGT TAGATCGATC 
GTCGATATTT CCATGTTCAT ACGTACGAGG CAAGACAGGG GCGACATATT TTATCTTGGA 
ACGGAAAATC CGAATCCGCA GACGGATCTT AAACCTCAAG AGAAAACGTA CATAGCTGCT 
CAACTGGAAG GGGGCGAGTT ACTTGTCAGG ATTCAGTTCA ATGGCACGGA AGCTTACACG 
GTTGGCGGTG TGAAATTGAA CGATGGAAAT AATCACTTGA TACAAGTGAT CAGGAACGTG 
ACGTTGGTGC AAGTGAAAAT TAACGGTACA GAATATTTCC GGAAAACTAT CAGCGCTTCC 
GGCCAGTTGA ACGTAACCGT CTTGTATTTG GGCGGTTTGC CACAAGCATC CAGATACATA 
AGGCAAGTCG ATAATCGTCA GATAGAAGTG TCCCAAGCTC CTCAAGTGAA CTTCAAAGGA 
ATCATTCAAG ATGTTCAGAT ATCCAACGGT GTTGAAACTA TGGTCGTTGA ATTCTTTCCT 
CTAAAGGCAA ACGATATTCC CACTCCGATA CAATTTGGCA ACGTGACATT TGATTATGAG 
AAAATTCTGA AAGGTGTGAT ATCCGACAAC GTATGCGTGA GCAACCCGTG CAATCACAAT 
GGAACTTGTC ACATCACATG GAACGATTTT TGGTGTCAAT GTCCACGAGG ATACACCGGG 
AAAACTTGCC AAGAGATGGA ATTTTGTCAA TTGCAAGACT GTCCCGCTGG ATCGAGATGT 
CAAAATTTAG ACGATGGCTA CGAGTGCATA GCCAATGCCA CATTTAACGG AGTTACCACT 
TCCTTCACCT ATGTTTATAA TCAAGTGGAA GAAAAAAATG TGACCGATTC GACAATCGAC 
ACTATTCAAA TTACGTATCG ATCGAATACC GGTGGCACGT TGATGCATAT CTCGCCTCGT 
ATAGGCGATC AACATTTCAC CGTTTCCGTT TATAAGGACA AGATCACGGT ATCTTGGCGG 
CTAGATTTAC AAAATGAAGG GATATTGAGC TTTGGTAAAA CCGAAACTGA CGGCAATTGG 
ACGTCAATAG TATTACGATT GAACAACAAT TCCATGGAAT GCGGATATGC AAATTCTAAC 
GATGAATATG CATCTCATGT CAGTCCAAAT TTTAGCTTTC CATTATGGTA TGATTTGCTA 
GTTACCGGAA CAGTCACTCT TGGCGGGCTT GGCTCTATTT TATCAAATAA ACACAGTTAC 
ATAACAATCG GTTCCAATAA ACAGAGCTTG GAAAACAAAA GTGGTATTAA CGAGAATTCG 
ATAGATTTCA CTGAACGTAC CACGTTGACC ACCGCTTCTC CCCCTTATAA CATAATGTCA 
GGGGAAGCTT TCAAAGGATG TTTAGGCGAA GTAAGAATTG GTAGTATGCT TTTACATTAC 
TTCACGTATG AAGAAGTATA TCAGAACGCT AATTTTACGC CATTAGAATA TTTAGCGTTA 
CAAGAAAATA ATTCAACGTA TCACGATAGT ATTGGATGCC AGCTTTGTTT CGATTCAGAT 
TGTAAAAATA ATGGATTTTG TCTGGACAAA GCAAACAGTT ACATTTGTGA ATGTCCTGCG 
GGATATACAG AGGACGATTG TTCCTTCAAC ATAGATGAAT GCATTAATAA TAAATGCGAG 
AACGGAGCAA CATGTATCGA TGGAATTGCT AATTACACGT GTATATGTAA CAACGGTTGG 
CAAGGATGGT TATGCGATAG CGACATAAAC GAGTGTGTAA CTCTTAGTCC CTGTCAACAT 
GACGGAGTGT GTGTAAATCT TCCCGGATAT TTCCGTTGCG AATGTCCAGA TCAATTTACC 
GGTGAAAGAT GTGAGATTTT CCGATTAATT ACTTGTGAAA ATCAACCTTG CAAAAATGGT 
GGCAGTTGCA CAGATATTAT AAATCCACAA ACTGGTGATA ATTTCACTTG TACTTGTACG 
ACTGGTTACG AAGGTTCAAT TTGTGATGTT CCTTATTGCA TTGGACAAAA GTGTCAAAAT 
GGCGGCAAAT GTGATTTCTT ATATCAGACA CCTCGATGTA CCTGTTTACC CGGCTATTCT 
GGATTGTATT GTGAAATTAA TATCGATGAT TGCGCACCGG ATGCAGAAGG CAATGTACCG 
TGTAAAAACG ATGGAAAATG CTACGATGGT GTAAACAGTT TCACTTGTGA TTGTAATGAT 
ACCGGCTACA CAGGGCCCGA TTGTTTATTT GATATAGATG AATGTGGAAT TCCAATAACA 
GATTGTGGTC AAGGACGGTG CAAAAATTTA CCAGGAACTT ATCAATGCAT TTGTGACCCG 
GGTTACTGTG GATATAATTG TGGAATGATA GATCCTTGCA AACCGGATTA TTGTCAAAAT 
GGAGGTACAT GTAAATGCGG AGATAATGGT GGTTATAGGT GTGAATGCAC GCCACATTAT 
ACCGGACAAA ATTGTACAGA GACGGAAAAC TTTTTGGGTA GTCAAGCACT CGATATAGCG 
GTAATTGTTG GCCCTATCGT CGGATGTCTG TTTATTATTG CAGCTGGTTC TTTAGTTGCA 
TTATTTATGA TGGCTAGGAA AAAACGAGCT ACTCGAGGAA CGTACAGTCC AAGTGCTCAA 
GAATTCAGCA ATCCGCGTGT AGAAATGGAT AATGTGATGA AACCACCGCC GGAAGAGAGA 
TTAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC725591 ( XM_026439372.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026295157.1 Apis mellifera protein crumbs (LOC725591), transcript variant X2, mRNA.

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CloneID	OHd29286
Clone ID Related Accession (Same CDS sequence)	XM_026439372.1
Accession Version	XM_026439372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	protein crumbs isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037640.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCGC GATACGTCGT GGCAGTACTT ACAAGTTTAT TAGTAGTGAC CGACGAAGCG 
CCCCAAGATA TCAGACACAA GAATCACGAT GAACGGCCCA GAGAAGCTTA TTTCAATGGA 
TCGGCATTCC TGAAATTGAG CTCGTTCGTA TCTGTGCACA GGCACAGCGG CTTGAGCTTT 
CGAACTTGCG AGGGCGGCCG GCTCTTCACC CAAAAGTACG ACGAGGACTC GATCAGTCTG 
GAAGTGACCC CGGACGGACT TTTGTTCGCG GCGATTGTCG AGCAGCAGAG ATACAAAGCG 
AGATTGAACG CCAGGCTGTT GAACAACGCC TGGCACAATG TCAATCTATT CTTTAGACTC 
GGAAATCTCA CCTTGAACGC AGCGGGCCAC ACTCAGGTGA TCGCCAACGC GACTTACAAC 
GCTGCCATTC TCACGTTGCC CGATTACGAT ATGAACGGGT CTCTCGTCGT TGGAGAAGGG 
TTCAAAGGAT GTATCCTGCA GGGTCCGGGG ATTCTTTTTA ACGACACGAT GAATAACGGG 
GCAGTTTTTG GCCCCTGCCC CGCGGAAAAT AAAGGATGTC CGCCATGCTT GAGTGGGCCA 
TGCCGGAACG GTGGTACGTG CAACGAGGAT ATAAAAGGGG ATTTCAGCTG CGCCTGCAAG 
CCGGGCTTCA CCGGTGTTCA CTGCGAGTCG CAGCTCGGCG TAAGGCTGTG CGAGCAAAGT 
CCGTGCAGGA ACGACGGTGT TTGTTTAGCG GTGACCGAGA CCGAATATAA ATGCGATTGC 
CAGCCGGGAT GGAGTGGAAA AAATTGCGAA ACGAATATCA ACGAGTGCTC CCCGAATCCT 
TGCAGGCACG GCGGTGTTTG TATCGATGGT ATTAATAATT ATACGTGCAT ATGCGACAGG 
ACTGGATACG AAGGTGCGAA TTGCGAGGTG GACATAGACG AATGCTTGGC TAATCCTTGC 
TTGAACAACG GCGTGTGCTA CGACAATTAC GGCGGTTACA TATGCCACTG CCCGAATGGT 
TTCGAGGGGC AGAACTGCGA GTTGAACTTG AACGAGTGCT TGTCGAATCC TTGCAAACAT 
GGCGGCGATT GCGTGGACGA CGTCGGCTCG TATCACTGCA ATTGCCCGTC CGGATACGCC 
GGCCGGCACT GCGAGCTTAG AAGTTTGTGC GAGAACGCGG CTTGCCCCTC GAACAGCATC 
TGCGTGGAGG ACGCGCACGG GCCGCAATGC GTGTGTAATC CCGGATACAT GGGCAATCCC 
CCGAATTGCA CCATCAATTA TTGCGCCAAT AATCCGTGCA GCAACGGGGG CACTTGCACG 
AGCAACAAAG ACGGATTCAA TTGCACTTGC CCACCGGAAT GGAAAGGAAC GACATGCCTG 
TCATCCGCCT CGGATTGGTG TTCGGCTTGT TACAATGGAG GCAGTTGTCT CGAGACGCGT 
TTCGGTATTA TGTGTCAGTG CCCAAGATTT TGGACGGGAC CGCAATGCAA GGAACCGATC 
ACCTGTCGCG ATCTACCTTG CAAACAAGCT TCCGCTTGTC ACGATTATCC CGGCGGTTAT 
TATTGTACCT GTGAGGCAGG ATGGACGGGA CCTGAATGTT CCATCGACGT GGACGAGTGT 
TCCAGCGATC CTTGCCGTAA CGGCGGTATC TGCATTGATC AACAAAATAG CTATTATTGT 
CAATGTCTTC CAGGATACAC CGGTAAAAAT TGCCAGATCA ACGTGGACGA GTGTTTGTCC 
CAGCCCTGTC AAAATGGCGG CACTTGCATC GATCGAATCA ACGGATACAT ATGTAACTGC 
ACGAAAGACT TCATGGATGA AAATTGCGAG CGGGAGTACA ACGCTTGCGC AGCAAATCCT 
TGCCACAACA ACGGGAACTG CACGTTGATA CCGAGATCTC GGCGTGAATT TGTCTGCGAG 
TGTCCACGTG GCTTCGAAGG GAAAACTTGC GACGTAAACG TGGACGATTG TATCGACGTG 
ATATGCCCCG ATGGGCAGGT TTGCGTCGAC GGTATCGCCG GTTACGAGTG CAAATGTCGC 
GAGGGTTACA GAGATCCTAA TTGCACTTAC ATCGTCGACC ATTGCGCGTC AGTGTCTTGT 
AACAGCGGTA CGTGCGTAGA TCTTGGAGAT CAAGGATACG AGTGCAAGTG CAAGGATGGT 
TTCCAAGGAA AATTTTGCGA CGAAGATGTG AACGAATGCA ACGTGGAGGG CAGTTCCTTG 
TGCAATAACG GTATTTGCGT AAATACAGAC GGCAGCTACA ATTGTTTCTG CAGGCCAGGA 
TTTTCCGGGG ATCACTGCGA CATCGACATA GATGAATGCT TGTGCGGCCC GTGCAAGAAT 
AATGCCACGT GTATCGACGG TATCAATACG TTCGAATGCC AGTGCCCGCT TGGTTATTCC 
GGGAAAACGT GCGACGTGGA CGTAAACGAG TGCGAAAGTG ATCCCTGTTT AAACGGTGCG 
ACTTGCGTCG ATGAAATAGC CAGTTACATG TGCATTTGTT CACCCGGTTT CCGGGGGTTA 
AACTGCGAGA TAAACATCGA CGATTGCGAA CCCCTGCCGT GTTTAAACCA CGGCCAATGC 
ATCGACGGTA TAAACAATTA TACCTGCGAC TGTAGCAATA CCGGGTTCGA GGGGGCGCAT 
TGCGAGAAAA ATATCGACGA TTGCCGGTCC GGCCCATGCG TGAACGGTGC TCATTGCATA 
GACGATATCA AGAATTACAA ATGTCAGTGC TACGCCGGTT ATACCGGGAA AAATTGCGAA 
GTCGATATAA ACGAGTGCGA GAGTTCGCCC TGTCAATATA ACGGCACTTG TTTAGAGAGA 
TCCAAGATAG AGTTGTACAA GAGCGACGCG TTAACGTTAC CCTCGATATT CAATCAAGAA 
TTCAGTTACG CGAATGCGAG TGGATACGAG TGTCTTTGCG TGCAAGGTGT TACGGGGAAA 
AATTGCGAGG TAAATATCAA CGAATGTGAT AGTAATCCGT GCCAAGCCGG GACCTGCGTT 
GATCGTATCG GTGGTTACAC CTGCGAATGC GACGAAGGAT ACGAGGGTGA TCATTGTCAA 
CACGATATCG ATGAGTGCAA AAGATATTCG CCCTGCGAGC ATGGTGTGTG TACAGATGGA 
AGGGCCGATT ATACTTGTAC TTGCGAGCCG GAATATGGCG GTAAAAATTG TTCGGTGGAG 
TTGATCGGTT GCCAGGGAAA CGCTTGTCAA AATGGCGGTA CTTGTTGGCC TTATCTCGTC 
GACGAAACTA TACACAAATT CAATTGCACT TGTCCCAATG GATATCATGG AGAAATTTGT 
GATTATGTAA CTACAATGTC TTTGAACGGC AGCTCGTACG TTTTAGTAAA CACTACTCGA 
GACGAAGGAT ACGATATACA ATTTCGTTTT CGAACGACGT TGCCAAATGG ATTATTAGCT 
ATCGGGAAAG GGTCGACGTT TTACATCCTC GAACTCGTGA ACGGCAAGTT GAATTTGCAC 
TCGAGCCTTT TGAACAAATG GGAGGGCGTG TTTATCGGAA GCGGTTTGAA CGATTCTACT 
TGGCAAAAAG TATTCGTCGC GATCAATGCT ACGCATTTAG TGCTCTCAGC CAATGAGGAG 
CAAACTATCT ATCCAATCAG TTTGAACGAG GGTTCCAATT CAAATCATAC GTCTTTTCCA 
ATGACTTACG TTGGAGGCAC CACCAATTAT CTGCGACGAT TGACTCATGG TCCGTCTTTC 
TTCATCGGCT GTACCGAAGA TGTCGTTATT AATGGAGAAT GGGTGTACTC CGGCACCTTG 
TTGAAAACGG TGTACATGGA GGATATTGAA CCAGGATGTC CACGAGAGGC GCAATGTTCG 
CCAAATCGGT GTAAAAATGG TGGTGACTGT ACAGACAGAT GGCGTGATTT TTCCTGCAAA 
TGCGAGAGGC CATATCTTGG CCACACCTGT CAATACAACA TGACGGCGGC CACGTTTGGA 
TACGAGAATA TCACCAACGG ATACGTGACC GTAAAAGTGT CGGACATGGC CAGGCGAGCA 
GTTAGATCGA TCGTCGATAT TTCCATGTTC ATACGTACGA GGCAAGACAG GGGCGACATA 
TTTTATCTTG GAACGGAAAA TCCGAATCCG CAGACGGATC TTAAACCTCA AGAGAAAACG 
TACATAGCTG CTCAACTGGA AGGGGGCGAG TTACTTGTCA GGATTCAGTT CAATGGCACG 
GAAGCTTACA CGGTTGGCGG TGTGAAATTG AACGATGGAA ATAATCACTT GATACAAGTG 
ATCAGGAACG TGACGTTGGT GCAAGTGAAA ATTAACGGTA CAGAATATTT CCGGAAAACT 
ATCAGCGCTT CCGGCCAGTT GAACGTAACC GTCTTGTATT TGGGCGGTTT GCCACAAGCA 
TCCAGATACA TAAGGCAAGT CGATAATCGT CAGATAGAAG TGTCCCAAGC TCCTCAAGTG 
AACTTCAAAG GAATCATTCA AGATGTTCAG ATATCCAACG GTGTTGAAAC TATGGTCGTT 
GAATTCTTTC CTCTAAAGGC AAACGATATT CCCACTCCGA TACAATTTGG CAACGTGACA 
TTTGATTATG AGAAAATTCT GAAAGGTGTG ATATCCGACA ACGTATGCGT GAGCAACCCG 
TGCAATCACA ATGGAACTTG TCACATCACA TGGAACGATT TTTGGTGTCA ATGTCCACGA 
GGATACACCG GGAAAACTTG CCAAGAGATG GAATTTTGTC AATTGCAAGA CTGTCCCGCT 
GGATCGAGAT GTCAAAATTT AGACGATGGC TACGAGTGCA TAGCCAATGC CACATTTAAC 
GGAGTTACCA CTTCCTTCAC CTATGTTTAT AATCAAGTGG AAGAAAAAAA TGTGACCGAT 
TCGACAATCG ACACTATTCA AATTACGTAT CGATCGAATA CCGGTGGCAC GTTGATGCAT 
ATCTCGCCTC GTATAGGCGA TCAACATTTC ACCGTTTCCG TTTATAAGGA CAAGATCACG 
GTATCTTGGC GGCTAGATTT ACAAAATGAA GGGATATTGA GCTTTGGTAA AACCGAAACT 
GACGGCAATT GGACGTCAAT AGTATTACGA TTGAACAACA ATTCCATGGA ATGCGGATAT 
GCAAATTCTA ACGATGAATA TGCATCTCAT GTCAGTCCAA ATTTTAGCTT TCCATTATGG 
TATGATTTGC TAGTTACCGG AACAGTCACT CTTGGCGGGC TTGGCTCTAT TTTATCAAAT 
AAACACAGTT ACATAACAAT CGGTTCCAAT AAACAGAGCT TGGAAAACAA AAGTGGTATT 
AACGAGAATT CGATAGATTT CACTGAACGT ACCACGTTGA CCACCGCTTC TCCCCCTTAT 
AACATAATGT CAGGGGAAGC TTTCAAAGGA TGTTTAGGCG AAGTAAGAAT TGGTAGTATG 
CTTTTACATT ACTTCACGTA TGAAGAAGTA TATCAGAACG CTAATTTTAC GCCATTAGAA 
TATTTAGCGT TACAAGAAAA TAATTCAACG TATCACGATA GTATTGGATG CCAGCTTTGT 
TTCGATTCAG ATTGTAAAAA TAATGGATTT TGTCTGGACA AAGCAAACAG TTACATTTGT 
GAATGTCCTG CGGGATATAC AGAGGACGAT TGTTCCTTCA ACATAGATGA ATGCATTAAT 
AATAAATGCG AGAACGGAGC AACATGTATC GATGGAATTG CTAATTACAC GTGTATATGT 
AACAACGGTT GGCAAGGATG GTTATGCGAT AGCGACATAA ACGAGTGTGT AACTCTTAGT 
CCCTGTCAAC ATGACGGAGT GTGTGTAAAT CTTCCCGGAT ATTTCCGTTG CGAATGTCCA 
GATCAATTTA CCGGTGAAAG ATGTGAGATT TTCCGATTAA TTACTTGTGA AAATCAACCT 
TGCAAAAATG GTGGCAGTTG CACAGATATT ATAAATCCAC AAACTGGTGA TAATTTCACT 
TGTACTTGTA CGACTGGTTA CGAAGGTTCA ATTTGTGATG TTCCTTATTG CATTGGACAA 
AAGTGTCAAA ATGGCGGCAA ATGTGATTTC TTATATCAGA CACCTCGATG TACCTGTTTA 
CCCGGCTATT CTGGATTGTA TTGTGAAATT AATATCGATG ATTGCGCACC GGATGCAGAA 
GGCAATGTAC CGTGTAAAAA CGATGGAAAA TGCTACGATG GTGTAAACAG TTTCACTTGT 
GATTGTAATG ATACCGGCTA CACAGGGCCC GATTGTTTAT TTGATATAGA TGAATGTGGA 
ATTCCAATAA CAGATTGTGG TCAAGGACGG TGCAAAAATT TACCAGGAAC TTATCAATGC 
ATTTGTGACC CGGGTTACTG TGGATATAAT TGTGGAATGA TAGATCCTTG CAAACCGGAT 
TATTGTCAAA ATGGAGGTAC ATGTAAATGC GGAGATAATG GTGGTTATAG GTGTGAATGC 
ACGCCACATT ATACCGGACA AAATTGTACA GAGACGGAAA ACTTTTTGGG TAGTCAAGCA 
CTCGATATAG CGGTAATTGT TGGCCCTATC GTCGGATGTC TGTTTATTAT TGCAGCTGGT 
TCTTTAGTTG CATTATTTAT GATGGCTAGG AAAAAACGAG CTACTCGAGG AACGTACAGT 
CCAAGTGCTC AAGAATTCAG CAATCCGCGT GTAGAAATGG ATAATGTGAT GAAACCACCG 
CCGGAAGAGA GATTAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026295157.1
CDS	330..7010
Translation	MRARYVVAVLTSLLVVTDEAPQDIRHKNHDERPREAYFNGSAFLKLSSFVSVHRHSGLSFRTCEGGRLFTQKYDEDSISLEVTPDGLLFAAIVEQQRYKARLNARLLNNAWHNVNLFFRLGNLTLNAAGHTQVIANATYNAAILTLPDYDMNGSLVVGEGFKGCILQGPGILFNDTMNNGAVFGPCPAENKGCPPCLSGPCRNGGTCNEDIKGDFSCACKPGFTGVHCESQLGVRLCEQSPCRNDGVCLAVTETEYKCDCQPGWSGKNCETNINECSPNPCRHGGVCIDGINNYTCICDRTGYEGANCEVDIDECLANPCLNNGVCYDNYGGYICHCPNGFEGQNCELNLNECLSNPCKHGGDCVDDVGSYHCNCPSGYAGRHCELRSLCENAACPSNSICVEDAHGPQCVCNPGYMGNPPNCTINYCANNPCSNGGTCTSNKDGFNCTCPPEWKGTTCLSSASDWCSACYNGGSCLETRFGIMCQCPRFWTGPQCKEPITCRDLPCKQASACHDYPGGYYCTCEAGWTGPECSIDVDECSSDPCRNGGICIDQQNSYYCQCLPGYTGKNCQINVDECLSQPCQNGGTCIDRINGYICNCTKDFMDENCEREYNACAANPCHNNGNCTLIPRSRREFVCECPRGFEGKTCDVNVDDCIDVICPDGQVCVDGIAGYECKCREGYRDPNCTYIVDHCASVSCNSGTCVDLGDQGYECKCKDGFQGKFCDEDVNECNVEGSSLCNNGICVNTDGSYNCFCRPGFSGDHCDIDIDECLCGPCKNNATCIDGINTFECQCPLGYSGKTCDVDVNECESDPCLNGATCVDEIASYMCICSPGFRGLNCEINIDDCEPLPCLNHGQCIDGINNYTCDCSNTGFEGAHCEKNIDDCRSGPCVNGAHCIDDIKNYKCQCYAGYTGKNCEVDINECESSPCQYNGTCLERSKIELYKSDALTLPSIFNQEFSYANASGYECLCVQGVTGKNCEVNINECDSNPCQAGTCVDRIGGYTCECDEGYEGDHCQHDIDECKRYSPCEHGVCTDGRADYTCTCEPEYGGKNCSVELIGCQGNACQNGGTCWPYLVDETIHKFNCTCPNGYHGEICDYVTTMSLNGSSYVLVNTTRDEGYDIQFRFRTTLPNGLLAIGKGSTFYILELVNGKLNLHSSLLNKWEGVFIGSGLNDSTWQKVFVAINATHLVLSANEEQTIYPISLNEGSNSNHTSFPMTYVGGTTNYLRRLTHGPSFFIGCTEDVVINGEWVYSGTLLKTVYMEDIEPGCPREAQCSPNRCKNGGDCTDRWRDFSCKCERPYLGHTCQYNMTAATFGYENITNGYVTVKVSDMARRAVRSIVDISMFIRTRQDRGDIFYLGTENPNPQTDLKPQEKTYIAAQLEGGELLVRIQFNGTEAYTVGGVKLNDGNNHLIQVIRNVTLVQVKINGTEYFRKTISASGQLNVTVLYLGGLPQASRYIRQVDNRQIEVSQAPQVNFKGIIQDVQISNGVETMVVEFFPLKANDIPTPIQFGNVTFDYEKILKGVISDNVCVSNPCNHNGTCHITWNDFWCQCPRGYTGKTCQEMEFCQLQDCPAGSRCQNLDDGYECIANATFNGVTTSFTYVYNQVEEKNVTDSTIDTIQITYRSNTGGTLMHISPRIGDQHFTVSVYKDKITVSWRLDLQNEGILSFGKTETDGNWTSIVLRLNNNSMECGYANSNDEYASHVSPNFSFPLWYDLLVTGTVTLGGLGSILSNKHSYITIGSNKQSLENKSGINENSIDFTERTTLTTASPPYNIMSGEAFKGCLGEVRIGSMLLHYFTYEEVYQNANFTPLEYLALQENNSTYHDSIGCQLCFDSDCKNNGFCLDKANSYICECPAGYTEDDCSFNIDECINNKCENGATCIDGIANYTCICNNGWQGWLCDSDINECVTLSPCQHDGVCVNLPGYFRCECPDQFTGERCEIFRLITCENQPCKNGGSCTDIINPQTGDNFTCTCTTGYEGSICDVPYCIGQKCQNGGKCDFLYQTPRCTCLPGYSGLYCEINIDDCAPDAEGNVPCKNDGKCYDGVNSFTCDCNDTGYTGPDCLFDIDECGIPITDCGQGRCKNLPGTYQCICDPGYCGYNCGMIDPCKPDYCQNGGTCKCGDNGGYRCECTPHYTGQNCTETENFLGSQALDIAVIVGPIVGCLFIIAAGSLVALFMMARKKRATRGTYSPSAQEFSNPRVEMDNVMKPPPEERLI

Target ORF information:

RefSeq Version	XM_026439372.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera protein crumbs (LOC725591), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026439372.1

ORF Insert Sequence:

ATGCGGGCGC GATACGTCGT GGCAGTACTT ACAAGTTTAT TAGTAGTGAC CGACGAAGCG 
CCCCAAGATA TCAGACACAA GAATCACGAT GAACGGCCCA GAGAAGCTTA TTTCAATGGA 
TCGGCATTCC TGAAATTGAG CTCGTTCGTA TCTGTGCACA GGCACAGCGG CTTGAGCTTT 
CGAACTTGCG AGGGCGGCCG GCTCTTCACC CAAAAGTACG ACGAGGACTC GATCAGTCTG 
GAAGTGACCC CGGACGGACT TTTGTTCGCG GCGATTGTCG AGCAGCAGAG ATACAAAGCG 
AGATTGAACG CCAGGCTGTT GAACAACGCC TGGCACAATG TCAATCTATT CTTTAGACTC 
GGAAATCTCA CCTTGAACGC AGCGGGCCAC ACTCAGGTGA TCGCCAACGC GACTTACAAC 
GCTGCCATTC TCACGTTGCC CGATTACGAT ATGAACGGGT CTCTCGTCGT TGGAGAAGGG 
TTCAAAGGAT GTATCCTGCA GGGTCCGGGG ATTCTTTTTA ACGACACGAT GAATAACGGG 
GCAGTTTTTG GCCCCTGCCC CGCGGAAAAT AAAGGATGTC CGCCATGCTT GAGTGGGCCA 
TGCCGGAACG GTGGTACGTG CAACGAGGAT ATAAAAGGGG ATTTCAGCTG CGCCTGCAAG 
CCGGGCTTCA CCGGTGTTCA CTGCGAGTCG CAGCTCGGCG TAAGGCTGTG CGAGCAAAGT 
CCGTGCAGGA ACGACGGTGT TTGTTTAGCG GTGACCGAGA CCGAATATAA ATGCGATTGC 
CAGCCGGGAT GGAGTGGAAA AAATTGCGAA ACGAATATCA ACGAGTGCTC CCCGAATCCT 
TGCAGGCACG GCGGTGTTTG TATCGATGGT ATTAATAATT ATACGTGCAT ATGCGACAGG 
ACTGGATACG AAGGTGCGAA TTGCGAGGTG GACATAGACG AATGCTTGGC TAATCCTTGC 
TTGAACAACG GCGTGTGCTA CGACAATTAC GGCGGTTACA TATGCCACTG CCCGAATGGT 
TTCGAGGGGC AGAACTGCGA GTTGAACTTG AACGAGTGCT TGTCGAATCC TTGCAAACAT 
GGCGGCGATT GCGTGGACGA CGTCGGCTCG TATCACTGCA ATTGCCCGTC CGGATACGCC 
GGCCGGCACT GCGAGCTTAG AAGTTTGTGC GAGAACGCGG CTTGCCCCTC GAACAGCATC 
TGCGTGGAGG ACGCGCACGG GCCGCAATGC GTGTGTAATC CCGGATACAT GGGCAATCCC 
CCGAATTGCA CCATCAATTA TTGCGCCAAT AATCCGTGCA GCAACGGGGG CACTTGCACG 
AGCAACAAAG ACGGATTCAA TTGCACTTGC CCACCGGAAT GGAAAGGAAC GACATGCCTG 
TCATCCGCCT CGGATTGGTG TTCGGCTTGT TACAATGGAG GCAGTTGTCT CGAGACGCGT 
TTCGGTATTA TGTGTCAGTG CCCAAGATTT TGGACGGGAC CGCAATGCAA GGAACCGATC 
ACCTGTCGCG ATCTACCTTG CAAACAAGCT TCCGCTTGTC ACGATTATCC CGGCGGTTAT 
TATTGTACCT GTGAGGCAGG ATGGACGGGA CCTGAATGTT CCATCGACGT GGACGAGTGT 
TCCAGCGATC CTTGCCGTAA CGGCGGTATC TGCATTGATC AACAAAATAG CTATTATTGT 
CAATGTCTTC CAGGATACAC CGGTAAAAAT TGCCAGATCA ACGTGGACGA GTGTTTGTCC 
CAGCCCTGTC AAAATGGCGG CACTTGCATC GATCGAATCA ACGGATACAT ATGTAACTGC 
ACGAAAGACT TCATGGATGA AAATTGCGAG CGGGAGTACA ACGCTTGCGC AGCAAATCCT 
TGCCACAACA ACGGGAACTG CACGTTGATA CCGAGATCTC GGCGTGAATT TGTCTGCGAG 
TGTCCACGTG GCTTCGAAGG GAAAACTTGC GACGTAAACG TGGACGATTG TATCGACGTG 
ATATGCCCCG ATGGGCAGGT TTGCGTCGAC GGTATCGCCG GTTACGAGTG CAAATGTCGC 
GAGGGTTACA GAGATCCTAA TTGCACTTAC ATCGTCGACC ATTGCGCGTC AGTGTCTTGT 
AACAGCGGTA CGTGCGTAGA TCTTGGAGAT CAAGGATACG AGTGCAAGTG CAAGGATGGT 
TTCCAAGGAA AATTTTGCGA CGAAGATGTG AACGAATGCA ACGTGGAGGG CAGTTCCTTG 
TGCAATAACG GTATTTGCGT AAATACAGAC GGCAGCTACA ATTGTTTCTG CAGGCCAGGA 
TTTTCCGGGG ATCACTGCGA CATCGACATA GATGAATGCT TGTGCGGCCC GTGCAAGAAT 
AATGCCACGT GTATCGACGG TATCAATACG TTCGAATGCC AGTGCCCGCT TGGTTATTCC 
GGGAAAACGT GCGACGTGGA CGTAAACGAG TGCGAAAGTG ATCCCTGTTT AAACGGTGCG 
ACTTGCGTCG ATGAAATAGC CAGTTACATG TGCATTTGTT CACCCGGTTT CCGGGGGTTA 
AACTGCGAGA TAAACATCGA CGATTGCGAA CCCCTGCCGT GTTTAAACCA CGGCCAATGC 
ATCGACGGTA TAAACAATTA TACCTGCGAC TGTAGCAATA CCGGGTTCGA GGGGGCGCAT 
TGCGAGAAAA ATATCGACGA TTGCCGGTCC GGCCCATGCG TGAACGGTGC TCATTGCATA 
GACGATATCA AGAATTACAA ATGTCAGTGC TACGCCGGTT ATACCGGGAA AAATTGCGAA 
GTCGATATAA ACGAGTGCGA GAGTTCGCCC TGTCAATATA ACGGCACTTG TTTAGAGAGA 
TCCAAGATAG AGTTGTACAA GAGCGACGCG TTAACGTTAC CCTCGATATT CAATCAAGAA 
TTCAGTTACG CGAATGCGAG TGGATACGAG TGTCTTTGCG TGCAAGGTGT TACGGGGAAA 
AATTGCGAGG TAAATATCAA CGAATGTGAT AGTAATCCGT GCCAAGCCGG GACCTGCGTT 
GATCGTATCG GTGGTTACAC CTGCGAATGC GACGAAGGAT ACGAGGGTGA TCATTGTCAA 
CACGATATCG ATGAGTGCAA AAGATATTCG CCCTGCGAGC ATGGTGTGTG TACAGATGGA 
AGGGCCGATT ATACTTGTAC TTGCGAGCCG GAATATGGCG GTAAAAATTG TTCGGTGGAG 
TTGATCGGTT GCCAGGGAAA CGCTTGTCAA AATGGCGGTA CTTGTTGGCC TTATCTCGTC 
GACGAAACTA TACACAAATT CAATTGCACT TGTCCCAATG GATATCATGG AGAAATTTGT 
GATTATGTAA CTACAATGTC TTTGAACGGC AGCTCGTACG TTTTAGTAAA CACTACTCGA 
GACGAAGGAT ACGATATACA ATTTCGTTTT CGAACGACGT TGCCAAATGG ATTATTAGCT 
ATCGGGAAAG GGTCGACGTT TTACATCCTC GAACTCGTGA ACGGCAAGTT GAATTTGCAC 
TCGAGCCTTT TGAACAAATG GGAGGGCGTG TTTATCGGAA GCGGTTTGAA CGATTCTACT 
TGGCAAAAAG TATTCGTCGC GATCAATGCT ACGCATTTAG TGCTCTCAGC CAATGAGGAG 
CAAACTATCT ATCCAATCAG TTTGAACGAG GGTTCCAATT CAAATCATAC GTCTTTTCCA 
ATGACTTACG TTGGAGGCAC CACCAATTAT CTGCGACGAT TGACTCATGG TCCGTCTTTC 
TTCATCGGCT GTACCGAAGA TGTCGTTATT AATGGAGAAT GGGTGTACTC CGGCACCTTG 
TTGAAAACGG TGTACATGGA GGATATTGAA CCAGGATGTC CACGAGAGGC GCAATGTTCG 
CCAAATCGGT GTAAAAATGG TGGTGACTGT ACAGACAGAT GGCGTGATTT TTCCTGCAAA 
TGCGAGAGGC CATATCTTGG CCACACCTGT CAATACAACA TGACGGCGGC CACGTTTGGA 
TACGAGAATA TCACCAACGG ATACGTGACC GTAAAAGTGT CGGACATGGC CAGGCGAGCA 
GTTAGATCGA TCGTCGATAT TTCCATGTTC ATACGTACGA GGCAAGACAG GGGCGACATA 
TTTTATCTTG GAACGGAAAA TCCGAATCCG CAGACGGATC TTAAACCTCA AGAGAAAACG 
TACATAGCTG CTCAACTGGA AGGGGGCGAG TTACTTGTCA GGATTCAGTT CAATGGCACG 
GAAGCTTACA CGGTTGGCGG TGTGAAATTG AACGATGGAA ATAATCACTT GATACAAGTG 
ATCAGGAACG TGACGTTGGT GCAAGTGAAA ATTAACGGTA CAGAATATTT CCGGAAAACT 
ATCAGCGCTT CCGGCCAGTT GAACGTAACC GTCTTGTATT TGGGCGGTTT GCCACAAGCA 
TCCAGATACA TAAGGCAAGT CGATAATCGT CAGATAGAAG TGTCCCAAGC TCCTCAAGTG 
AACTTCAAAG GAATCATTCA AGATGTTCAG ATATCCAACG GTGTTGAAAC TATGGTCGTT 
GAATTCTTTC CTCTAAAGGC AAACGATATT CCCACTCCGA TACAATTTGG CAACGTGACA 
TTTGATTATG AGAAAATTCT GAAAGGTGTG ATATCCGACA ACGTATGCGT GAGCAACCCG 
TGCAATCACA ATGGAACTTG TCACATCACA TGGAACGATT TTTGGTGTCA ATGTCCACGA 
GGATACACCG GGAAAACTTG CCAAGAGATG GAATTTTGTC AATTGCAAGA CTGTCCCGCT 
GGATCGAGAT GTCAAAATTT AGACGATGGC TACGAGTGCA TAGCCAATGC CACATTTAAC 
GGAGTTACCA CTTCCTTCAC CTATGTTTAT AATCAAGTGG AAGAAAAAAA TGTGACCGAT 
TCGACAATCG ACACTATTCA AATTACGTAT CGATCGAATA CCGGTGGCAC GTTGATGCAT 
ATCTCGCCTC GTATAGGCGA TCAACATTTC ACCGTTTCCG TTTATAAGGA CAAGATCACG 
GTATCTTGGC GGCTAGATTT ACAAAATGAA GGGATATTGA GCTTTGGTAA AACCGAAACT 
GACGGCAATT GGACGTCAAT AGTATTACGA TTGAACAACA ATTCCATGGA ATGCGGATAT 
GCAAATTCTA ACGATGAATA TGCATCTCAT GTCAGTCCAA ATTTTAGCTT TCCATTATGG 
TATGATTTGC TAGTTACCGG AACAGTCACT CTTGGCGGGC TTGGCTCTAT TTTATCAAAT 
AAACACAGTT ACATAACAAT CGGTTCCAAT AAACAGAGCT TGGAAAACAA AAGTGGTATT 
AACGAGAATT CGATAGATTT CACTGAACGT ACCACGTTGA CCACCGCTTC TCCCCCTTAT 
AACATAATGT CAGGGGAAGC TTTCAAAGGA TGTTTAGGCG AAGTAAGAAT TGGTAGTATG 
CTTTTACATT ACTTCACGTA TGAAGAAGTA TATCAGAACG CTAATTTTAC GCCATTAGAA 
TATTTAGCGT TACAAGAAAA TAATTCAACG TATCACGATA GTATTGGATG CCAGCTTTGT 
TTCGATTCAG ATTGTAAAAA TAATGGATTT TGTCTGGACA AAGCAAACAG TTACATTTGT 
GAATGTCCTG CGGGATATAC AGAGGACGAT TGTTCCTTCA ACATAGATGA ATGCATTAAT 
AATAAATGCG AGAACGGAGC AACATGTATC GATGGAATTG CTAATTACAC GTGTATATGT 
AACAACGGTT GGCAAGGATG GTTATGCGAT AGCGACATAA ACGAGTGTGT AACTCTTAGT 
CCCTGTCAAC ATGACGGAGT GTGTGTAAAT CTTCCCGGAT ATTTCCGTTG CGAATGTCCA 
GATCAATTTA CCGGTGAAAG ATGTGAGATT TTCCGATTAA TTACTTGTGA AAATCAACCT 
TGCAAAAATG GTGGCAGTTG CACAGATATT ATAAATCCAC AAACTGGTGA TAATTTCACT 
TGTACTTGTA CGACTGGTTA CGAAGGTTCA ATTTGTGATG TTCCTTATTG CATTGGACAA 
AAGTGTCAAA ATGGCGGCAA ATGTGATTTC TTATATCAGA CACCTCGATG TACCTGTTTA 
CCCGGCTATT CTGGATTGTA TTGTGAAATT AATATCGATG ATTGCGCACC GGATGCAGAA 
GGCAATGTAC CGTGTAAAAA CGATGGAAAA TGCTACGATG GTGTAAACAG TTTCACTTGT 
GATTGTAATG ATACCGGCTA CACAGGGCCC GATTGTTTAT TTGATATAGA TGAATGTGGA 
ATTCCAATAA CAGATTGTGG TCAAGGACGG TGCAAAAATT TACCAGGAAC TTATCAATGC 
ATTTGTGACC CGGGTTACTG TGGATATAAT TGTGGAATGA TAGATCCTTG CAAACCGGAT 
TATTGTCAAA ATGGAGGTAC ATGTAAATGC GGAGATAATG GTGGTTATAG GTGTGAATGC 
ACGCCACATT ATACCGGACA AAATTGTACA GAGACGGAAA ACTTTTTGGG TAGTCAAGCA 
CTCGATATAG CGGTAATTGT TGGCCCTATC GTCGGATGTC TGTTTATTAT TGCAGCTGGT 
TCTTTAGTTG CATTATTTAT GATGGCTAGG AAAAAACGAG CTACTCGAGG AACGTACAGT 
CCAAGTGCTC AAGAATTCAG CAATCCGCGT GTAGAAATGG ATAATGTGAT GAAACCACCG 
CCGGAAGAGA GATTAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC725591 ( XM_026439374.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026295159.1 Apis mellifera protein crumbs (LOC725591), transcript variant X3, mRNA.

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CloneID	OHd29287
Clone ID Related Accession (Same CDS sequence)	XM_026439374.1
Accession Version	XM_026439374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	protein crumbs isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037640.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCGC GATACGTCGT GGCAGTACTT ACAAGTTTAT TAGTAGTGAC CGACGAAGCG 
CCCCAAGATA TCAGACACAA GAATCACGAT GAACGGCCCA GAGAAGCTTA TTTCAATGGA 
TCGGCATTCC TGAAATTGAG CTCGTTCGTA TCTGTGCACA GGCACAGCGG CTTGAGCTTT 
CGAACTTGCG AGGGCGGCCG GCTCTTCACC CAAAAGTACG ACGAGGACTC GATCAGTCTG 
GAAGTGACCC CGGACGGACT TTTGTTCGCG GCGATTGTCG AGCAGCAGAG ATACAAAGCG 
AGATTGAACG CCAGGCTGTT GAACAACGCC TGGCACAATG TCAATCTATT CTTTAGACTC 
GGAAATCTCA CCTTGAACGC AGCGGGCCAC ACTCAGGTGA TCGCCAACGC GACTTACAAC 
GCTGCCATTC TCACGTTGCC CGATTACGAT ATGAACGGGT CTCTCGTCGT TGGAGAAGGG 
TTCAAAGGAT GTATCCTGCA GGGTCCGGGG ATTCTTTTTA ACGACACGAT GAATAACGGG 
GCAGTTTTTG GCCCCTGCCC CGCGGAAAAT AAAGGATGTG GTACGAGCGG ACTTAACGGT 
GGTGTAGGAT CAAGCGTAAT CACCACCATG GATTTCTGCT ATCTCGAGCC ATGCATGATG 
CATGGTAAAT GCGTGTCACG GCAGGATCGT TACGAGTGTC ACTGTTACGC CCGATATTCA 
GGCAACAACT GTCAAATTGA CAACGGTCCG CCATGCTTGA GTGGGCCATG CCGGAACGGT 
GGTACGTGCA ACGAGGATAT AAAAGGGGAT TTCAGCTGCG CCTGCAAGCC GGGCTTCACC 
GGTGTTCACT GCGAGTCGCA GCTCGGCGTA AGGCTGTGCG AGCAAAGTCC GTGCAGGAAC 
GACGGTGTTT GTTTAGCGGT GACCGAGACC GAATATAAAT GCGATTGCCA GCCGGGATGG 
AGTGGAAAAA ATTGCGAAAC GAATATCAAC GAGTGCTCCC CGAATCCTTG CAGGCACGGC 
GGTGTTTGTA TCGATGGTAT TAATAATTAT ACGTGCATAT GCGACAGGAC TGGATACGAA 
GGTGCGAATT GCGAGGTGGA CATAGACGAA TGCTTGGCTA ATCCTTGCTT GAACAACGGC 
GTGTGCTACG ACAATTACGG CGGTTACATA TGCCACTGCC CGAATGGTTT CGAGGGGCAG 
AACTGCGAGT TGAACTTGAA CGAGTGCTTG TCGAATCCTT GCAAACATGG CGGCGATTGC 
GTGGACGACG TCGGCTCGTA TCACTGCAAT TGCCCGTCCG GATACGCCGG CCGGCACTGC 
GAGCTTAGAA GTTTGTGCGA GAACGCGGCT TGCCCCTCGA ACAGCATCTG CGTGGAGGAC 
GCGCACGGGC CGCAATGCGT GTGTAATCCC GGATACATGG GCAATCCCCC GAATTGCACC 
ATCAATTATT GCGCCAATAA TCCGTGCAGC AACGGGGGCA CTTGCACGAG CAACAAAGAC 
GGATTCAATT GCACTTGCCC ACCGGAATGG AAAGGTAAAA ATTGCCAGAT CAACGTGGAC 
GAGTGTTTGT CCCAGCCCTG TCAAAATGGC GGCACTTGCA TCGATCGAAT CAACGGATAC 
ATATGTAACT GCACGAAAGA CTTCATGGAT GAAAATTGCG AGCGGGAGTA CAACGCTTGC 
GCAGCAAATC CTTGCCACAA CAACGGGAAC TGCACGTTGA TACCGAGATC TCGGCGTGAA 
TTTGTCTGCG AGTGTCCACG TGGCTTCGAA GGGAAAACTT GCGACGTAAA CGTGGACGAT 
TGTATCGACG TGATATGCCC CGATGGGCAG GTTTGCGTCG ACGGTATCGC CGGTTACGAG 
TGCAAATGTC GCGAGGGTTA CAGAGATCCT AATTGCACTT ACATCGTCGA CCATTGCGCG 
TCAGTGTCTT GTAACAGCGG TACGTGCGTA GATCTTGGAG ATCAAGGATA CGAGTGCAAG 
TGCAAGGATG GTTTCCAAGG AAAATTTTGC GACGAAGATG TGAACGAATG CAACGTGGAG 
GGCAGTTCCT TGTGCAATAA CGGTATTTGC GTAAATACAG ACGGCAGCTA CAATTGTTTC 
TGCAGGCCAG GATTTTCCGG GGATCACTGC GACATCGACA TAGATGAATG CTTGTGCGGC 
CCGTGCAAGA ATAATGCCAC GTGTATCGAC GGTATCAATA CGTTCGAATG CCAGTGCCCG 
CTTGGTTATT CCGGGAAAAC GTGCGACGTG GACGTAAACG AGTGCGAAAG TGATCCCTGT 
TTAAACGGTG CGACTTGCGT CGATGAAATA GCCAGTTACA TGTGCATTTG TTCACCCGGT 
TTCCGGGGGT TAAACTGCGA GATAAACATC GACGATTGCG AACCCCTGCC GTGTTTAAAC 
CACGGCCAAT GCATCGACGG TATAAACAAT TATACCTGCG ACTGTAGCAA TACCGGGTTC 
GAGGGGGCGC ATTGCGAGAA AAATATCGAC GATTGCCGGT CCGGCCCATG CGTGAACGGT 
GCTCATTGCA TAGACGATAT CAAGAATTAC AAATGTCAGT GCTACGCCGG TTATACCGGG 
AAAAATTGCG AAGTCGATAT AAACGAGTGC GAGAGTTCGC CCTGTCAATA TAACGGCACT 
TGTTTAGAGA GATCCAAGAT AGAGTTGTAC AAGAGCGACG CGTTAACGTT ACCCTCGATA 
TTCAATCAAG AATTCAGTTA CGCGAATGCG AGTGGATACG AGTGTCTTTG CGTGCAAGGT 
GTTACGGGGA AAAATTGCGA GGTAAATATC AACGAATGTG ATAGTAATCC GTGCCAAGCC 
GGGACCTGCG TTGATCGTAT CGGTGGTTAC ACCTGCGAAT GCGACGAAGG ATACGAGGGT 
GATCATTGTC AACACGATAT CGATGAGTGC AAAAGATATT CGCCCTGCGA GCATGGTGTG 
TGTACAGATG GAAGGGCCGA TTATACTTGT ACTTGCGAGC CGGAATATGG CGGTAAAAAT 
TGTTCGGTGG AGTTGATCGG TTGCCAGGGA AACGCTTGTC AAAATGGCGG TACTTGTTGG 
CCTTATCTCG TCGACGAAAC TATACACAAA TTCAATTGCA CTTGTCCCAA TGGATATCAT 
GGAGAAATTT GTGATTATGT AACTACAATG TCTTTGAACG GCAGCTCGTA CGTTTTAGTA 
AACACTACTC GAGACGAAGG ATACGATATA CAATTTCGTT TTCGAACGAC GTTGCCAAAT 
GGATTATTAG CTATCGGGAA AGGGTCGACG TTTTACATCC TCGAACTCGT GAACGGCAAG 
TTGAATTTGC ACTCGAGCCT TTTGAACAAA TGGGAGGGCG TGTTTATCGG AAGCGGTTTG 
AACGATTCTA CTTGGCAAAA AGTATTCGTC GCGATCAATG CTACGCATTT AGTGCTCTCA 
GCCAATGAGG AGCAAACTAT CTATCCAATC AGTTTGAACG AGGGTTCCAA TTCAAATCAT 
ACGTCTTTTC CAATGACTTA CGTTGGAGGC ACCACCAATT ATCTGCGACG ATTGACTCAT 
GGTCCGTCTT TCTTCATCGG CTGTACCGAA GATGTCGTTA TTAATGGAGA ATGGGTGTAC 
TCCGGCACCT TGTTGAAAAC GGTGTACATG GAGGATATTG AACCAGGATG TCCACGAGAG 
GCGCAATGTT CGCCAAATCG GTGTAAAAAT GGTGGTGACT GTACAGACAG ATGGCGTGAT 
TTTTCCTGCA AATGCGAGAG GCCATATCTT GGCCACACCT GTCAATACAA CATGACGGCG 
GCCACGTTTG GATACGAGAA TATCACCAAC GGATACGTGA CCGTAAAAGT GTCGGACATG 
GCCAGGCGAG CAGTTAGATC GATCGTCGAT ATTTCCATGT TCATACGTAC GAGGCAAGAC 
AGGGGCGACA TATTTTATCT TGGAACGGAA AATCCGAATC CGCAGACGGA TCTTAAACCT 
CAAGAGAAAA CGTACATAGC TGCTCAACTG GAAGGGGGCG AGTTACTTGT CAGGATTCAG 
TTCAATGGCA CGGAAGCTTA CACGGTTGGC GGTGTGAAAT TGAACGATGG AAATAATCAC 
TTGATACAAG TGATCAGGAA CGTGACGTTG GTGCAAGTGA AAATTAACGG TACAGAATAT 
TTCCGGAAAA CTATCAGCGC TTCCGGCCAG TTGAACGTAA CCGTCTTGTA TTTGGGCGGT 
TTGCCACAAG CATCCAGATA CATAAGGCAA GTCGATAATC GTCAGATAGA AGTGTCCCAA 
GCTCCTCAAG TGAACTTCAA AGGAATCATT CAAGATGTTC AGATATCCAA CGGTGTTGAA 
ACTATGGTCG TTGAATTCTT TCCTCTAAAG GCAAACGATA TTCCCACTCC GATACAATTT 
GGCAACGTGA CATTTGATTA TGAGAAAATT CTGAAAGGTG TGATATCCGA CAACGTATGC 
GTGAGCAACC CGTGCAATCA CAATGGAACT TGTCACATCA CATGGAACGA TTTTTGGTGT 
CAATGTCCAC GAGGATACAC CGGGAAAACT TGCCAAGAGA TGGAATTTTG TCAATTGCAA 
GACTGTCCCG CTGGATCGAG ATGTCAAAAT TTAGACGATG GCTACGAGTG CATAGCCAAT 
GCCACATTTA ACGGAGTTAC CACTTCCTTC ACCTATGTTT ATAATCAAGT GGAAGAAAAA 
AATGTGACCG ATTCGACAAT CGACACTATT CAAATTACGT ATCGATCGAA TACCGGTGGC 
ACGTTGATGC ATATCTCGCC TCGTATAGGC GATCAACATT TCACCGTTTC CGTTTATAAG 
GACAAGATCA CGGTATCTTG GCGGCTAGAT TTACAAAATG AAGGGATATT GAGCTTTGGT 
AAAACCGAAA CTGACGGCAA TTGGACGTCA ATAGTATTAC GATTGAACAA CAATTCCATG 
GAATGCGGAT ATGCAAATTC TAACGATGAA TATGCATCTC ATGTCAGTCC AAATTTTAGC 
TTTCCATTAT GGTATGATTT GCTAGTTACC GGAACAGTCA CTCTTGGCGG GCTTGGCTCT 
ATTTTATCAA ATAAACACAG TTACATAACA ATCGGTTCCA ATAAACAGAG CTTGGAAAAC 
AAAAGTGGTA TTAACGAGAA TTCGATAGAT TTCACTGAAC GTACCACGTT GACCACCGCT 
TCTCCCCCTT ATAACATAAT GTCAGGGGAA GCTTTCAAAG GATGTTTAGG CGAAGTAAGA 
ATTGGTAGTA TGCTTTTACA TTACTTCACG TATGAAGAAG TATATCAGAA CGCTAATTTT 
ACGCCATTAG AATATTTAGC GTTACAAGAA AATAATTCAA CGTATCACGA TAGTATTGGA 
TGCCAGCTTT GTTTCGATTC AGATTGTAAA AATAATGGAT TTTGTCTGGA CAAAGCAAAC 
AGTTACATTT GTGAATGTCC TGCGGGATAT ACAGAGGACG ATTGTTCCTT CAACATAGAT 
GAATGCATTA ATAATAAATG CGAGAACGGA GCAACATGTA TCGATGGAAT TGCTAATTAC 
ACGTGTATAT GTAACAACGG TTGGCAAGGA TGGTTATGCG ATAGCGACAT AAACGAGTGT 
GTAACTCTTA GTCCCTGTCA ACATGACGGA GTGTGTGTAA ATCTTCCCGG ATATTTCCGT 
TGCGAATGTC CAGATCAATT TACCGGTGAA AGATGTGAGA TTTTCCGATT AATTACTTGT 
GAAAATCAAC CTTGCAAAAA TGGTGGCAGT TGCACAGATA TTATAAATCC ACAAACTGGT 
GATAATTTCA CTTGTACTTG TACGACTGGT TACGAAGGTT CAATTTGTGA TGTTCCTTAT 
TGCATTGGAC AAAAGTGTCA AAATGGCGGC AAATGTGATT TCTTATATCA GACACCTCGA 
TGTACCTGTT TACCCGGCTA TTCTGGATTG TATTGTGAAA TTAATATCGA TGATTGCGCA 
CCGGATGCAG AAGGCAATGT ACCGTGTAAA AACGATGGAA AATGCTACGA TGGTGTAAAC 
AGTTTCACTT GTGATTGTAA TGATACCGGC TACACAGGGC CCGATTGTTT ATTTGATATA 
GATGAATGTG GAATTCCAAT AACAGATTGT GGTCAAGGAC GGTGCAAAAA TTTACCAGGA 
ACTTATCAAT GCATTTGTGA CCCGGGTTAC TGTGGATATA ATTGTGGAAT GATAGATCCT 
TGCAAACCGG ATTATTGTCA AAATGGAGGT ACATGTAAAT GCGGAGATAA TGGTGGTTAT 
AGGTGTGAAT GCACGCCACA TTATACCGGA CAAAATTGTA CAGAGACGGA AAACTTTTTG 
GGTAGTCAAG CACTCGATAT AGCGGTAATT GTTGGCCCTA TCGTCGGATG TCTGTTTATT 
ATTGCAGCTG GTTCTTTAGT TGCATTATTT ATGATGGCTA GGAAAAAACG AGCTACTCGA 
GGAACGTACA GTCCAAGTGC TCAAGAATTC AGCAATCCGC GTGTAGAAAT GGATAATGTG 
ATGAAACCAC CGCCGGAAGA GAGATTAATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026295159.1
CDS	326..6838
Translation	MRARYVVAVLTSLLVVTDEAPQDIRHKNHDERPREAYFNGSAFLKLSSFVSVHRHSGLSFRTCEGGRLFTQKYDEDSISLEVTPDGLLFAAIVEQQRYKARLNARLLNNAWHNVNLFFRLGNLTLNAAGHTQVIANATYNAAILTLPDYDMNGSLVVGEGFKGCILQGPGILFNDTMNNGAVFGPCPAENKGCGTSGLNGGVGSSVITTMDFCYLEPCMMHGKCVSRQDRYECHCYARYSGNNCQIDNGPPCLSGPCRNGGTCNEDIKGDFSCACKPGFTGVHCESQLGVRLCEQSPCRNDGVCLAVTETEYKCDCQPGWSGKNCETNINECSPNPCRHGGVCIDGINNYTCICDRTGYEGANCEVDIDECLANPCLNNGVCYDNYGGYICHCPNGFEGQNCELNLNECLSNPCKHGGDCVDDVGSYHCNCPSGYAGRHCELRSLCENAACPSNSICVEDAHGPQCVCNPGYMGNPPNCTINYCANNPCSNGGTCTSNKDGFNCTCPPEWKGKNCQINVDECLSQPCQNGGTCIDRINGYICNCTKDFMDENCEREYNACAANPCHNNGNCTLIPRSRREFVCECPRGFEGKTCDVNVDDCIDVICPDGQVCVDGIAGYECKCREGYRDPNCTYIVDHCASVSCNSGTCVDLGDQGYECKCKDGFQGKFCDEDVNECNVEGSSLCNNGICVNTDGSYNCFCRPGFSGDHCDIDIDECLCGPCKNNATCIDGINTFECQCPLGYSGKTCDVDVNECESDPCLNGATCVDEIASYMCICSPGFRGLNCEINIDDCEPLPCLNHGQCIDGINNYTCDCSNTGFEGAHCEKNIDDCRSGPCVNGAHCIDDIKNYKCQCYAGYTGKNCEVDINECESSPCQYNGTCLERSKIELYKSDALTLPSIFNQEFSYANASGYECLCVQGVTGKNCEVNINECDSNPCQAGTCVDRIGGYTCECDEGYEGDHCQHDIDECKRYSPCEHGVCTDGRADYTCTCEPEYGGKNCSVELIGCQGNACQNGGTCWPYLVDETIHKFNCTCPNGYHGEICDYVTTMSLNGSSYVLVNTTRDEGYDIQFRFRTTLPNGLLAIGKGSTFYILELVNGKLNLHSSLLNKWEGVFIGSGLNDSTWQKVFVAINATHLVLSANEEQTIYPISLNEGSNSNHTSFPMTYVGGTTNYLRRLTHGPSFFIGCTEDVVINGEWVYSGTLLKTVYMEDIEPGCPREAQCSPNRCKNGGDCTDRWRDFSCKCERPYLGHTCQYNMTAATFGYENITNGYVTVKVSDMARRAVRSIVDISMFIRTRQDRGDIFYLGTENPNPQTDLKPQEKTYIAAQLEGGELLVRIQFNGTEAYTVGGVKLNDGNNHLIQVIRNVTLVQVKINGTEYFRKTISASGQLNVTVLYLGGLPQASRYIRQVDNRQIEVSQAPQVNFKGIIQDVQISNGVETMVVEFFPLKANDIPTPIQFGNVTFDYEKILKGVISDNVCVSNPCNHNGTCHITWNDFWCQCPRGYTGKTCQEMEFCQLQDCPAGSRCQNLDDGYECIANATFNGVTTSFTYVYNQVEEKNVTDSTIDTIQITYRSNTGGTLMHISPRIGDQHFTVSVYKDKITVSWRLDLQNEGILSFGKTETDGNWTSIVLRLNNNSMECGYANSNDEYASHVSPNFSFPLWYDLLVTGTVTLGGLGSILSNKHSYITIGSNKQSLENKSGINENSIDFTERTTLTTASPPYNIMSGEAFKGCLGEVRIGSMLLHYFTYEEVYQNANFTPLEYLALQENNSTYHDSIGCQLCFDSDCKNNGFCLDKANSYICECPAGYTEDDCSFNIDECINNKCENGATCIDGIANYTCICNNGWQGWLCDSDINECVTLSPCQHDGVCVNLPGYFRCECPDQFTGERCEIFRLITCENQPCKNGGSCTDIINPQTGDNFTCTCTTGYEGSICDVPYCIGQKCQNGGKCDFLYQTPRCTCLPGYSGLYCEINIDDCAPDAEGNVPCKNDGKCYDGVNSFTCDCNDTGYTGPDCLFDIDECGIPITDCGQGRCKNLPGTYQCICDPGYCGYNCGMIDPCKPDYCQNGGTCKCGDNGGYRCECTPHYTGQNCTETENFLGSQALDIAVIVGPIVGCLFIIAAGSLVALFMMARKKRATRGTYSPSAQEFSNPRVEMDNVMKPPPEERLI

Target ORF information:

RefSeq Version	XM_026439374.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera protein crumbs (LOC725591), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026439374.1

ORF Insert Sequence:

ATGCGGGCGC GATACGTCGT GGCAGTACTT ACAAGTTTAT TAGTAGTGAC CGACGAAGCG 
CCCCAAGATA TCAGACACAA GAATCACGAT GAACGGCCCA GAGAAGCTTA TTTCAATGGA 
TCGGCATTCC TGAAATTGAG CTCGTTCGTA TCTGTGCACA GGCACAGCGG CTTGAGCTTT 
CGAACTTGCG AGGGCGGCCG GCTCTTCACC CAAAAGTACG ACGAGGACTC GATCAGTCTG 
GAAGTGACCC CGGACGGACT TTTGTTCGCG GCGATTGTCG AGCAGCAGAG ATACAAAGCG 
AGATTGAACG CCAGGCTGTT GAACAACGCC TGGCACAATG TCAATCTATT CTTTAGACTC 
GGAAATCTCA CCTTGAACGC AGCGGGCCAC ACTCAGGTGA TCGCCAACGC GACTTACAAC 
GCTGCCATTC TCACGTTGCC CGATTACGAT ATGAACGGGT CTCTCGTCGT TGGAGAAGGG 
TTCAAAGGAT GTATCCTGCA GGGTCCGGGG ATTCTTTTTA ACGACACGAT GAATAACGGG 
GCAGTTTTTG GCCCCTGCCC CGCGGAAAAT AAAGGATGTG GTACGAGCGG ACTTAACGGT 
GGTGTAGGAT CAAGCGTAAT CACCACCATG GATTTCTGCT ATCTCGAGCC ATGCATGATG 
CATGGTAAAT GCGTGTCACG GCAGGATCGT TACGAGTGTC ACTGTTACGC CCGATATTCA 
GGCAACAACT GTCAAATTGA CAACGGTCCG CCATGCTTGA GTGGGCCATG CCGGAACGGT 
GGTACGTGCA ACGAGGATAT AAAAGGGGAT TTCAGCTGCG CCTGCAAGCC GGGCTTCACC 
GGTGTTCACT GCGAGTCGCA GCTCGGCGTA AGGCTGTGCG AGCAAAGTCC GTGCAGGAAC 
GACGGTGTTT GTTTAGCGGT GACCGAGACC GAATATAAAT GCGATTGCCA GCCGGGATGG 
AGTGGAAAAA ATTGCGAAAC GAATATCAAC GAGTGCTCCC CGAATCCTTG CAGGCACGGC 
GGTGTTTGTA TCGATGGTAT TAATAATTAT ACGTGCATAT GCGACAGGAC TGGATACGAA 
GGTGCGAATT GCGAGGTGGA CATAGACGAA TGCTTGGCTA ATCCTTGCTT GAACAACGGC 
GTGTGCTACG ACAATTACGG CGGTTACATA TGCCACTGCC CGAATGGTTT CGAGGGGCAG 
AACTGCGAGT TGAACTTGAA CGAGTGCTTG TCGAATCCTT GCAAACATGG CGGCGATTGC 
GTGGACGACG TCGGCTCGTA TCACTGCAAT TGCCCGTCCG GATACGCCGG CCGGCACTGC 
GAGCTTAGAA GTTTGTGCGA GAACGCGGCT TGCCCCTCGA ACAGCATCTG CGTGGAGGAC 
GCGCACGGGC CGCAATGCGT GTGTAATCCC GGATACATGG GCAATCCCCC GAATTGCACC 
ATCAATTATT GCGCCAATAA TCCGTGCAGC AACGGGGGCA CTTGCACGAG CAACAAAGAC 
GGATTCAATT GCACTTGCCC ACCGGAATGG AAAGGTAAAA ATTGCCAGAT CAACGTGGAC 
GAGTGTTTGT CCCAGCCCTG TCAAAATGGC GGCACTTGCA TCGATCGAAT CAACGGATAC 
ATATGTAACT GCACGAAAGA CTTCATGGAT GAAAATTGCG AGCGGGAGTA CAACGCTTGC 
GCAGCAAATC CTTGCCACAA CAACGGGAAC TGCACGTTGA TACCGAGATC TCGGCGTGAA 
TTTGTCTGCG AGTGTCCACG TGGCTTCGAA GGGAAAACTT GCGACGTAAA CGTGGACGAT 
TGTATCGACG TGATATGCCC CGATGGGCAG GTTTGCGTCG ACGGTATCGC CGGTTACGAG 
TGCAAATGTC GCGAGGGTTA CAGAGATCCT AATTGCACTT ACATCGTCGA CCATTGCGCG 
TCAGTGTCTT GTAACAGCGG TACGTGCGTA GATCTTGGAG ATCAAGGATA CGAGTGCAAG 
TGCAAGGATG GTTTCCAAGG AAAATTTTGC GACGAAGATG TGAACGAATG CAACGTGGAG 
GGCAGTTCCT TGTGCAATAA CGGTATTTGC GTAAATACAG ACGGCAGCTA CAATTGTTTC 
TGCAGGCCAG GATTTTCCGG GGATCACTGC GACATCGACA TAGATGAATG CTTGTGCGGC 
CCGTGCAAGA ATAATGCCAC GTGTATCGAC GGTATCAATA CGTTCGAATG CCAGTGCCCG 
CTTGGTTATT CCGGGAAAAC GTGCGACGTG GACGTAAACG AGTGCGAAAG TGATCCCTGT 
TTAAACGGTG CGACTTGCGT CGATGAAATA GCCAGTTACA TGTGCATTTG TTCACCCGGT 
TTCCGGGGGT TAAACTGCGA GATAAACATC GACGATTGCG AACCCCTGCC GTGTTTAAAC 
CACGGCCAAT GCATCGACGG TATAAACAAT TATACCTGCG ACTGTAGCAA TACCGGGTTC 
GAGGGGGCGC ATTGCGAGAA AAATATCGAC GATTGCCGGT CCGGCCCATG CGTGAACGGT 
GCTCATTGCA TAGACGATAT CAAGAATTAC AAATGTCAGT GCTACGCCGG TTATACCGGG 
AAAAATTGCG AAGTCGATAT AAACGAGTGC GAGAGTTCGC CCTGTCAATA TAACGGCACT 
TGTTTAGAGA GATCCAAGAT AGAGTTGTAC AAGAGCGACG CGTTAACGTT ACCCTCGATA 
TTCAATCAAG AATTCAGTTA CGCGAATGCG AGTGGATACG AGTGTCTTTG CGTGCAAGGT 
GTTACGGGGA AAAATTGCGA GGTAAATATC AACGAATGTG ATAGTAATCC GTGCCAAGCC 
GGGACCTGCG TTGATCGTAT CGGTGGTTAC ACCTGCGAAT GCGACGAAGG ATACGAGGGT 
GATCATTGTC AACACGATAT CGATGAGTGC AAAAGATATT CGCCCTGCGA GCATGGTGTG 
TGTACAGATG GAAGGGCCGA TTATACTTGT ACTTGCGAGC CGGAATATGG CGGTAAAAAT 
TGTTCGGTGG AGTTGATCGG TTGCCAGGGA AACGCTTGTC AAAATGGCGG TACTTGTTGG 
CCTTATCTCG TCGACGAAAC TATACACAAA TTCAATTGCA CTTGTCCCAA TGGATATCAT 
GGAGAAATTT GTGATTATGT AACTACAATG TCTTTGAACG GCAGCTCGTA CGTTTTAGTA 
AACACTACTC GAGACGAAGG ATACGATATA CAATTTCGTT TTCGAACGAC GTTGCCAAAT 
GGATTATTAG CTATCGGGAA AGGGTCGACG TTTTACATCC TCGAACTCGT GAACGGCAAG 
TTGAATTTGC ACTCGAGCCT TTTGAACAAA TGGGAGGGCG TGTTTATCGG AAGCGGTTTG 
AACGATTCTA CTTGGCAAAA AGTATTCGTC GCGATCAATG CTACGCATTT AGTGCTCTCA 
GCCAATGAGG AGCAAACTAT CTATCCAATC AGTTTGAACG AGGGTTCCAA TTCAAATCAT 
ACGTCTTTTC CAATGACTTA CGTTGGAGGC ACCACCAATT ATCTGCGACG ATTGACTCAT 
GGTCCGTCTT TCTTCATCGG CTGTACCGAA GATGTCGTTA TTAATGGAGA ATGGGTGTAC 
TCCGGCACCT TGTTGAAAAC GGTGTACATG GAGGATATTG AACCAGGATG TCCACGAGAG 
GCGCAATGTT CGCCAAATCG GTGTAAAAAT GGTGGTGACT GTACAGACAG ATGGCGTGAT 
TTTTCCTGCA AATGCGAGAG GCCATATCTT GGCCACACCT GTCAATACAA CATGACGGCG 
GCCACGTTTG GATACGAGAA TATCACCAAC GGATACGTGA CCGTAAAAGT GTCGGACATG 
GCCAGGCGAG CAGTTAGATC GATCGTCGAT ATTTCCATGT TCATACGTAC GAGGCAAGAC 
AGGGGCGACA TATTTTATCT TGGAACGGAA AATCCGAATC CGCAGACGGA TCTTAAACCT 
CAAGAGAAAA CGTACATAGC TGCTCAACTG GAAGGGGGCG AGTTACTTGT CAGGATTCAG 
TTCAATGGCA CGGAAGCTTA CACGGTTGGC GGTGTGAAAT TGAACGATGG AAATAATCAC 
TTGATACAAG TGATCAGGAA CGTGACGTTG GTGCAAGTGA AAATTAACGG TACAGAATAT 
TTCCGGAAAA CTATCAGCGC TTCCGGCCAG TTGAACGTAA CCGTCTTGTA TTTGGGCGGT 
TTGCCACAAG CATCCAGATA CATAAGGCAA GTCGATAATC GTCAGATAGA AGTGTCCCAA 
GCTCCTCAAG TGAACTTCAA AGGAATCATT CAAGATGTTC AGATATCCAA CGGTGTTGAA 
ACTATGGTCG TTGAATTCTT TCCTCTAAAG GCAAACGATA TTCCCACTCC GATACAATTT 
GGCAACGTGA CATTTGATTA TGAGAAAATT CTGAAAGGTG TGATATCCGA CAACGTATGC 
GTGAGCAACC CGTGCAATCA CAATGGAACT TGTCACATCA CATGGAACGA TTTTTGGTGT 
CAATGTCCAC GAGGATACAC CGGGAAAACT TGCCAAGAGA TGGAATTTTG TCAATTGCAA 
GACTGTCCCG CTGGATCGAG ATGTCAAAAT TTAGACGATG GCTACGAGTG CATAGCCAAT 
GCCACATTTA ACGGAGTTAC CACTTCCTTC ACCTATGTTT ATAATCAAGT GGAAGAAAAA 
AATGTGACCG ATTCGACAAT CGACACTATT CAAATTACGT ATCGATCGAA TACCGGTGGC 
ACGTTGATGC ATATCTCGCC TCGTATAGGC GATCAACATT TCACCGTTTC CGTTTATAAG 
GACAAGATCA CGGTATCTTG GCGGCTAGAT TTACAAAATG AAGGGATATT GAGCTTTGGT 
AAAACCGAAA CTGACGGCAA TTGGACGTCA ATAGTATTAC GATTGAACAA CAATTCCATG 
GAATGCGGAT ATGCAAATTC TAACGATGAA TATGCATCTC ATGTCAGTCC AAATTTTAGC 
TTTCCATTAT GGTATGATTT GCTAGTTACC GGAACAGTCA CTCTTGGCGG GCTTGGCTCT 
ATTTTATCAA ATAAACACAG TTACATAACA ATCGGTTCCA ATAAACAGAG CTTGGAAAAC 
AAAAGTGGTA TTAACGAGAA TTCGATAGAT TTCACTGAAC GTACCACGTT GACCACCGCT 
TCTCCCCCTT ATAACATAAT GTCAGGGGAA GCTTTCAAAG GATGTTTAGG CGAAGTAAGA 
ATTGGTAGTA TGCTTTTACA TTACTTCACG TATGAAGAAG TATATCAGAA CGCTAATTTT 
ACGCCATTAG AATATTTAGC GTTACAAGAA AATAATTCAA CGTATCACGA TAGTATTGGA 
TGCCAGCTTT GTTTCGATTC AGATTGTAAA AATAATGGAT TTTGTCTGGA CAAAGCAAAC 
AGTTACATTT GTGAATGTCC TGCGGGATAT ACAGAGGACG ATTGTTCCTT CAACATAGAT 
GAATGCATTA ATAATAAATG CGAGAACGGA GCAACATGTA TCGATGGAAT TGCTAATTAC 
ACGTGTATAT GTAACAACGG TTGGCAAGGA TGGTTATGCG ATAGCGACAT AAACGAGTGT 
GTAACTCTTA GTCCCTGTCA ACATGACGGA GTGTGTGTAA ATCTTCCCGG ATATTTCCGT 
TGCGAATGTC CAGATCAATT TACCGGTGAA AGATGTGAGA TTTTCCGATT AATTACTTGT 
GAAAATCAAC CTTGCAAAAA TGGTGGCAGT TGCACAGATA TTATAAATCC ACAAACTGGT 
GATAATTTCA CTTGTACTTG TACGACTGGT TACGAAGGTT CAATTTGTGA TGTTCCTTAT 
TGCATTGGAC AAAAGTGTCA AAATGGCGGC AAATGTGATT TCTTATATCA GACACCTCGA 
TGTACCTGTT TACCCGGCTA TTCTGGATTG TATTGTGAAA TTAATATCGA TGATTGCGCA 
CCGGATGCAG AAGGCAATGT ACCGTGTAAA AACGATGGAA AATGCTACGA TGGTGTAAAC 
AGTTTCACTT GTGATTGTAA TGATACCGGC TACACAGGGC CCGATTGTTT ATTTGATATA 
GATGAATGTG GAATTCCAAT AACAGATTGT GGTCAAGGAC GGTGCAAAAA TTTACCAGGA 
ACTTATCAAT GCATTTGTGA CCCGGGTTAC TGTGGATATA ATTGTGGAAT GATAGATCCT 
TGCAAACCGG ATTATTGTCA AAATGGAGGT ACATGTAAAT GCGGAGATAA TGGTGGTTAT 
AGGTGTGAAT GCACGCCACA TTATACCGGA CAAAATTGTA CAGAGACGGA AAACTTTTTG 
GGTAGTCAAG CACTCGATAT AGCGGTAATT GTTGGCCCTA TCGTCGGATG TCTGTTTATT 
ATTGCAGCTG GTTCTTTAGT TGCATTATTT ATGATGGCTA GGAAAAAACG AGCTACTCGA 
GGAACGTACA GTCCAAGTGC TCAAGAATTC AGCAATCCGC GTGTAGAAAT GGATAATGTG 
ATGAAACCAC CGCCGGAAGA GAGATTAATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC725591 cDNA ORF clone, Apis mellifera(honey bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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