nAChRa9 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	nAChRa9
Entrez Gene ID	411303
Full Name	nicotinic acetylcholine receptor alpha9 subunit
Synonyms	GB16984
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)
Genome	Chromosome: LG9 Map Location: LG9\|LG9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001098224.2	NP_001091694.1	nicotinic acetylcholine receptor alpha9 subunit precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Association of single nucleotide polymorphisms of nicotinic acetylcholine receptor subunits with cervical neoplasia.
Calleja-Macias I, Osann K, Remedios-Chan M, Barrera-Saldana HA, Illades-Aguiar B, Anton-Culver H, Chikova AK, Grando SA, Bernard HU
Life sciences91(21-22)1099-102(2012 Nov)

The nicotinic acetylcholine receptor gene family of the honey bee, Apis mellifera.
Jones AK, Raymond-Delpech V, Thany SH, Gauthier M, Sattelle DB
Genome research16(11)1422-30(2006 Nov)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nAChRa9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nAChRa9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00053	NM_001098224.2 Latest version!	Apis mellifera nicotinic acetylcholine receptor alpha9 subunit (nAChRa9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nAChRa9 ( NM_001098224.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001091694.1 Apis mellifera nicotinic acetylcholine receptor alpha9 subunit (nAChRa9), mRNA.

Price & Availability↑

CloneID	OHd00053
Clone ID Related Accession (Same CDS sequence)	NM_001098224.2
Accession Version	NM_001098224.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556812800000
Organism	Apis mellifera(honey bee)
Product	nicotinic acetylcholine receptor alpha9 subunit precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ026037.1. On Aug 31, 2012 this sequence version replaced NM_001098224.1. ##Evidence-Data-START## Transcript exon combination :: DQ026037.1, HP477588.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00008880, SAMN00113341 [ECO:0000350] ##Evidence-Data-END##

ATGAAAATGA GAATAATAAC AGCTCTTGGA TTCCTCCTTT TTTGGCAGAA TTCGGCAAGC 
TTAGAAAACG ACAATGAATT CGGATTCTCG TATCTGTTAA ATTGCAAGAA TTACGACCAT 
CCAACCACCT TGTTGAAATT GAAGAGATAT CTTTTCTGCG AGTATGATCC TAATGTCCGA 
CCGATCTCGT CCCATCAAAT CGCCAACAAC GTGACCATGC AACTACTTCC AAAACTGATG 
GAATTCGATG ATTGGACCAG CGTGATGGAA TTGCACAGTT GGATGACTCT CATGTGGACC 
GACTCTCATC TGTCATGGAA GCCTAGCGAC TTCGACGGGA TCAATTATAT TTACGTGAAG 
AGCGACGATA TTTGGGTTCC CGACATTTCC GTTTACAATT CAGGCGACAT GACGTTCGAT 
CAAACTGGTA TACCACCGAC CACGTGCCTG GTGTTCAGTT CGGGTTCGGT AAGCTGCGTA 
CCATCGGTGA AACACGTTGC CAAATGCGCG ACAGATTTCT CCTCGTGGCC CTACGACACT 
CATCGATGCC GGATTAATTT CGGCTCGTGG GTCCATTCAG GGGAGGAAGT GAACATTTTT 
CTCGACAAAA AGGGGTTCCA CATGGACGGG TATACGAATA ACTCGAAATG GGATTTCAAA 
GTGATAAAAG CGACCAAAGT GTTGAAGATG TACGCTTGTT GCCCGAACGA CACTTATCCG 
ATGATCGTTT ACGAATTCTC CATAAGCCGA CATTACGGCA TATTGCACGC CACCTACGTC 
ATACCTGCCG TAACGATGAT GTTGCTCACG TTGACGGTAT TATGGTTAGA CTCGAGATCA 
ACGGAGAGAA TGATCGCAGC GAGCGTGAAT TTGATCTGTC ATATACTTTG CATGTCCGAT 
TTGCATTGGC AATTGCCTCA TAATAGCACC AATCCTCCTA ATATACTGCT CTACTATAGA 
GATTCGCTTG CCTTGTCCGT ATTCGCTTTA ATTTTAACCG CTCTGCTTCG GAAAATGCAA 
GAAATGAGCA TCGAGGTGCC ATATTGGATT TCTACGACAA CATCGTTCGT CTTGAACAAC 
AGAGCCGGGC GTTTCCTTAT TCTTACCGAG AGCGATACCA AATTGTCCAG CCAGGGGATA 
CTTGGCGAAG ACGTTGAGAA CAATTCGGAA GTTTCGAAAT CTCGGACTAA GGAATCGGCG 
TGGAGACATT TCGCGGCCAT AATCGAGTGG CTCTCGTTCT TCATCGTGAT TTTCACTTAC 
ATCATCATTC TGATCACTCT TGTACCATCC ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091694.1
CDS	29..1324
Translation	MKMRIITALGFLLFWQNSASLENDNEFGFSYLLNCKNYDHPTTLLKLKRYLFCEYDPNVRPISSHQIANNVTMQLLPKLMEFDDWTSVMELHSWMTLMWTDSHLSWKPSDFDGINYIYVKSDDIWVPDISVYNSGDMTFDQTGIPPTTCLVFSSGSVSCVPSVKHVAKCATDFSSWPYDTHRCRINFGSWVHSGEEVNIFLDKKGFHMDGYTNNSKWDFKVIKATKVLKMYACCPNDTYPMIVYEFSISRHYGILHATYVIPAVTMMLLTLTVLWLDSRSTERMIAASVNLICHILCMSDLHWQLPHNSTNPPNILLYYRDSLALSVFALILTALLRKMQEMSIEVPYWISTTTSFVLNNRAGRFLILTESDTKLSSQGILGEDVENNSEVSKSRTKESAWRHFAAIIEWLSFFIVIFTYIIILITLVPST

Target ORF information:

RefSeq Version	NM_001098224.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera nicotinic acetylcholine receptor alpha9 subunit (nAChRa9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098224.2

ORF Insert Sequence:

ATGAAAATGA GAATAATAAC AGCTCTTGGA TTCCTCCTTT TTTGGCAGAA TTCGGCAAGC 
TTAGAAAACG ACAATGAATT CGGATTCTCG TATCTGTTAA ATTGCAAGAA TTACGACCAT 
CCAACCACCT TGTTGAAATT GAAGAGATAT CTTTTCTGCG AGTATGATCC TAATGTCCGA 
CCGATCTCGT CCCATCAAAT CGCCAACAAC GTGACCATGC AACTACTTCC AAAACTGATG 
GAATTCGATG ATTGGACCAG CGTGATGGAA TTGCACAGTT GGATGACTCT CATGTGGACC 
GACTCTCATC TGTCATGGAA GCCTAGCGAC TTCGACGGGA TCAATTATAT TTACGTGAAG 
AGCGACGATA TTTGGGTTCC CGACATTTCC GTTTACAATT CAGGCGACAT GACGTTCGAT 
CAAACTGGTA TACCACCGAC CACGTGCCTG GTGTTCAGTT CGGGTTCGGT AAGCTGCGTA 
CCATCGGTGA AACACGTTGC CAAATGCGCG ACAGATTTCT CCTCGTGGCC CTACGACACT 
CATCGATGCC GGATTAATTT CGGCTCGTGG GTCCATTCAG GGGAGGAAGT GAACATTTTT 
CTCGACAAAA AGGGGTTCCA CATGGACGGG TATACGAATA ACTCGAAATG GGATTTCAAA 
GTGATAAAAG CGACCAAAGT GTTGAAGATG TACGCTTGTT GCCCGAACGA CACTTATCCG 
ATGATCGTTT ACGAATTCTC CATAAGCCGA CATTACGGCA TATTGCACGC CACCTACGTC 
ATACCTGCCG TAACGATGAT GTTGCTCACG TTGACGGTAT TATGGTTAGA CTCGAGATCA 
ACGGAGAGAA TGATCGCAGC GAGCGTGAAT TTGATCTGTC ATATACTTTG CATGTCCGAT 
TTGCATTGGC AATTGCCTCA TAATAGCACC AATCCTCCTA ATATACTGCT CTACTATAGA 
GATTCGCTTG CCTTGTCCGT ATTCGCTTTA ATTTTAACCG CTCTGCTTCG GAAAATGCAA 
GAAATGAGCA TCGAGGTGCC ATATTGGATT TCTACGACAA CATCGTTCGT CTTGAACAAC 
AGAGCCGGGC GTTTCCTTAT TCTTACCGAG AGCGATACCA AATTGTCCAG CCAGGGGATA 
CTTGGCGAAG ACGTTGAGAA CAATTCGGAA GTTTCGAAAT CTCGGACTAA GGAATCGGCG 
TGGAGACATT TCGCGGCCAT AATCGAGTGG CTCTCGTTCT TCATCGTGAT TTTCACTTAC 
ATCATCATTC TGATCACTCT TGTACCATCC ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Association of single nucleotide polymorphisms of nicotinic acetylcholine receptor subunits with cervical neoplasia.
Life sciences91(21-22)1099-102(2012 Nov)
Calleja-Macias I,Osann K,Remedios-Chan M,Barrera-Saldana HA,Illades-Aguiar B,Anton-Culver H,Chikova AK,Grando SA,Bernard HU

The nicotinic acetylcholine receptor gene family of the honey bee, Apis mellifera.
Genome research16(11)1422-30(2006 Nov)
Jones AK,Raymond-Delpech V,Thany SH,Gauthier M,Sattelle DB

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

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nAChRa9 cDNA ORF clone, Apis mellifera(honey bee)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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