crh-BP cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	crh-BP
Entrez Gene ID	409372
Full Name	corticotropin releasing hormone binding protein
Synonyms	GB14094
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012615.1	NP_001012633.1	corticotropin releasing hormone binding protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

The remarkable conservation of corticotropin-releasing hormone (CRH)-binding protein in the honeybee (Apis mellifera) dates the CRH system to a common ancestor of insects and vertebrates.
Huising MO, Flik G
Endocrinology146(5)2165-70(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following crh-BP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crh-BP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00270	NM_001012615.1 Latest version!	Apis mellifera corticotropin releasing hormone binding protein (crh-BP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crh-BP ( NM_001012615.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001012633.1 Apis mellifera corticotropin releasing hormone binding protein (crh-BP), mRNA.

Price & Availability↑

CloneID	OHd00270
Clone ID Related Accession (Same CDS sequence)	NM_001012615.1
Accession Version	NM_001012615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556812800000
Organism	Apis mellifera(honey bee)
Product	corticotropin releasing hormone binding protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ780964.2. On Mar 8, 2005 this sequence version replaced XM_392887.1. ##Evidence-Data-START## Transcript exon combination :: AJ780964.2, HP534973.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00008880, SAMN00113341 [ECO:0000350] ##Evidence-Data-END##

ATGTTCTTAA ATGGGTCTTT GTATTGTTTC GCGAGGTTGT TTTTTATCAT TGGATTTTTT 
AACGCTATTT CTGGTGCAAT AAAAGGGAAT GACTATCAGC GTTATCACCA ACAGATTTCT 
GGAGATCGTT TCTCAAAGGA TTTCTATCGT CAAGACACAA AAAATAATTT ATTAAATGCT 
TATGTGCGTT TTAAACTCGT CACAGACTGC ATATTCGTCA CATCAGAACC AGGATATTTC 
TTATATACTT CAAAAAATGA CAATGAGGAG GTCTGTGGAA TTTATTTTTT AGCTGAACCA 
GATCAGAAAA TAGAAATCAA CTTCATTACT TTTGATATAC CTTGTGAACA TCGTGGATTA 
GTTTCGATCA TTGATGGTTG GGAATTAAAC GGTGAAGTAT TCCCTAGTAA AATGGATCAT 
CAGTTACCAC TTAAACAAAG AAGTAGTGAA TTTTGTGGTA AAAATATCGG TATGAAAAGA 
ATTTTCACAA GTTCACAGAA CATTGCAGTT ATTGAATACA GAATTCCAAA ATCAGGAAAG 
GGATTCTCGT TGTTCGCGAG ATTCTTGAAA AATCCTAGAC CCTGTAATGT CTTAGCGACA 
AGCTTAACTG AGCCATATAC GTTACGAAAT TATGGACGAC GCATAAATTG CACTTATGTC 
GCGCTCTATC CGAGTTCTGT GCAAGTGATT GCATTGGGCG TTGGAGTTTC AAATTTTCTC 
AGTTCCACTC GCACTGCTGA GACCGGAACT ATACGAAAAT GTGATGAATC TAGTCCACAT 
GATCAAGTAA TAATTGGAGG TAGCAATGGA TTAGATACTT CTAAAGTTCA TATAATTGAT 
TCTATTTGTG GAATAGACTC TAAACCAGAT TATCGCGAAT TGACGGAATA TAGCGTTACT 
TCTGTTCGTT TAATATCAAG TGGTTTTTTC GATAATTTTG TAACAGTACA AATTCAACCT 
CTCAAGAACG AATTATTCAA TGCAAATATT GGTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012633.1
CDS	26..1024
Translation	MFLNGSLYCFARLFFIIGFFNAISGAIKGNDYQRYHQQISGDRFSKDFYRQDTKNNLLNAYVRFKLVTDCIFVTSEPGYFLYTSKNDNEEVCGIYFLAEPDQKIEINFITFDIPCEHRGLVSIIDGWELNGEVFPSKMDHQLPLKQRSSEFCGKNIGMKRIFTSSQNIAVIEYRIPKSGKGFSLFARFLKNPRPCNVLATSLTEPYTLRNYGRRINCTYVALYPSSVQVIALGVGVSNFLSSTRTAETGTIRKCDESSPHDQVIIGGSNGLDTSKVHIIDSICGIDSKPDYRELTEYSVTSVRLISSGFFDNFVTVQIQPLKNELFNANIGI

Target ORF information:

RefSeq Version	NM_001012615.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera corticotropin releasing hormone binding protein (crh-BP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012615.1

ORF Insert Sequence:

ATGTTCTTAA ATGGGTCTTT GTATTGTTTC GCGAGGTTGT TTTTTATCAT TGGATTTTTT 
AACGCTATTT CTGGTGCAAT AAAAGGGAAT GACTATCAGC GTTATCACCA ACAGATTTCT 
GGAGATCGTT TCTCAAAGGA TTTCTATCGT CAAGACACAA AAAATAATTT ATTAAATGCT 
TATGTGCGTT TTAAACTCGT CACAGACTGC ATATTCGTCA CATCAGAACC AGGATATTTC 
TTATATACTT CAAAAAATGA CAATGAGGAG GTCTGTGGAA TTTATTTTTT AGCTGAACCA 
GATCAGAAAA TAGAAATCAA CTTCATTACT TTTGATATAC CTTGTGAACA TCGTGGATTA 
GTTTCGATCA TTGATGGTTG GGAATTAAAC GGTGAAGTAT TCCCTAGTAA AATGGATCAT 
CAGTTACCAC TTAAACAAAG AAGTAGTGAA TTTTGTGGTA AAAATATCGG TATGAAAAGA 
ATTTTCACAA GTTCACAGAA CATTGCAGTT ATTGAATACA GAATTCCAAA ATCAGGAAAG 
GGATTCTCGT TGTTCGCGAG ATTCTTGAAA AATCCTAGAC CCTGTAATGT CTTAGCGACA 
AGCTTAACTG AGCCATATAC GTTACGAAAT TATGGACGAC GCATAAATTG CACTTATGTC 
GCGCTCTATC CGAGTTCTGT GCAAGTGATT GCATTGGGCG TTGGAGTTTC AAATTTTCTC 
AGTTCCACTC GCACTGCTGA GACCGGAACT ATACGAAAAT GTGATGAATC TAGTCCACAT 
GATCAAGTAA TAATTGGAGG TAGCAATGGA TTAGATACTT CTAAAGTTCA TATAATTGAT 
TCTATTTGTG GAATAGACTC TAAACCAGAT TATCGCGAAT TGACGGAATA TAGCGTTACT 
TCTGTTCGTT TAATATCAAG TGGTTTTTTC GATAATTTTG TAACAGTACA AATTCAACCT 
CTCAAGAACG AATTATTCAA TGCAAATATT GGTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

The remarkable conservation of corticotropin-releasing hormone (CRH)-binding protein in the honeybee (Apis mellifera) dates the CRH system to a common ancestor of insects and vertebrates.
Endocrinology146(5)2165-70(2005 May)
Huising MO,Flik G

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crh-BP cDNA ORF clone, Apis mellifera(honey bee)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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