Tk cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tk
Entrez Gene ID	406083
Full Name	tachykinin
Synonyms	Atk,GB12290,Trp
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001011576.1	NP_001011576.1	tachykinins precursor
XM_026441578.1	XP_026297363.1	tachykinins isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

From the genome to the proteome: uncovering peptides in the Apis brain.
Hummon AB, Richmond TA, Verleyen P, Baggerman G, Huybrechts J, Ewing MA, Vierstraete E, Rodriguez-Zas SL, Schoofs L, Robinson GE, Sweedler JV
Science (New York, N.Y.)314(5799)647-9(2006 Oct)

Prepro-tachykinin gene expression in the brain of the honeybee Apis mellifera.
Takeuchi H, Yasuda A, Yasuda-Kamatani Y, Sawata M, Matsuo Y, Kato A, Tsujimoto A, Nakajima T, Kubo T
Cell and tissue research316(2)281-93(2004 May)

Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads.
Takeuchi H, Yasuda A, Yasuda-Kamatani Y, Kubo T, Nakajima T
Insect molecular biology12(3)291-8(2003 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00437	NM_001011576.1 Latest version!	Apis mellifera tachykinin (Tk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHd32052	XM_026441578.1 Latest version!	Apis mellifera tachykinin (Tk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tk ( NM_001011576.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001011576.1 Apis mellifera tachykinin (Tk), mRNA.

Price & Availability↑

CloneID	OHd00437
Clone ID Related Accession (Same CDS sequence)	NM_001011576.1
Accession Version	NM_001011576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573660800000
Organism	Apis mellifera(honey bee)
Product	tachykinins precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB073998.1. On Feb 5, 2005 this sequence version replaced XM_393040.1. ##Evidence-Data-START## Transcript exon combination :: AB073998.1, HP540580.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00113341, SAMN00120364 [ECO:0000348] ##Evidence-Data-END##

ATGATCATCC ATTCGATCTT TTTGTTAATG GTATCAATCA CGCTCGTAAT TGCTGAAGAA 
TCTGACAATG TTTTATTCGA CAAACGTGCC CCTACGGGAC ATCAAGAAAT GCAAGGGAAA 
CAGAATTCTG CTTCTTTGAA CTCTGAAAAC TTTGGAATTT TCAAACGTGC GCTCATGGGT 
TTTCAGGGTG TGCGTGGAAA GAAAAACTCG ATAATCAATG ACGTCAAAAA TGAACTTTTT 
CCTGAGGACA TTAACAAGCG TGCACCGATG GGTTTTCAAG GCATGAGAGG GAAGAAGGCC 
TCGTTCGACG ATGAATATTA CAAACGTGCT CCGATGGGAT TTCAAGGAAT GAGAGGCAAA 
AAATCTCTTG AAGAGATTTT AGACGAGATT AAAAAGAAGA CTACGAGATT CCAGGATTCA 
AGAAGCAAAG ACGTGTACTT GATTGATTAT CCGGAAGATT ATGGAAAGAG AGTATTGTCG 
ATGGATGGAT ATCAAAATAT TCTCGATAAG AAGGACGAAT TACTGGGAGA GTGGGAAAAA 
AGAGCTCCGA TGGGATTTTA TGGAACGAGG GGTAAAAAAA TTATTCTTGA TGCGTTGGAG 
GAACTTGATA AACGAGGCGT TATGGATTTT CAAATAGGTT TGCAACGTAA GAAAGATACG 
ACGTTCGACG ATTATTTAGA TTATGCGATT AATCCGTTTG ATTACGAGAA AAGGAGTACG 
GATTTTCAAG ACGTAGAAAG CGGTAGCGAA TCCTTTAAAC GAGCTCGTAT GGGTTTTCAT 
GGGATGAGAG GTAAAAGGGA TGCGGCGGGG ATTTACGGAT CAAATTCAAG TACTGTGGGA 
ACGATTTTTG GTTATCAAGA TATGAGGAAT CGAGGAAATA ATTTTCCTGT ATATCAAGTT 
GAAAAACGAT CACCCTTCAG ATACCTTGGT GCCCGAGGTA AAAAGAATCC ACGATGGGAA 
TTTCGAGGGA AATTCGTAGG AGTGAGAGGC AAAAAATCGT CGTTGCAAAC TGTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011576.1
CDS	141..1160
Translation	MIIHSIFLLMVSITLVIAEESDNVLFDKRAPTGHQEMQGKQNSASLNSENFGIFKRALMGFQGVRGKKNSIINDVKNELFPEDINKRAPMGFQGMRGKKASFDDEYYKRAPMGFQGMRGKKSLEEILDEIKKKTTRFQDSRSKDVYLIDYPEDYGKRVLSMDGYQNILDKKDELLGEWEKRAPMGFYGTRGKKIILDALEELDKRGVMDFQIGLQRKKDTTFDDYLDYAINPFDYEKRSTDFQDVESGSESFKRARMGFHGMRGKRDAAGIYGSNSSTVGTIFGYQDMRNRGNNFPVYQVEKRSPFRYLGARGKKNPRWEFRGKFVGVRGKKSSLQTVF

Target ORF information:

RefSeq Version	NM_001011576.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera tachykinin (Tk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011576.1

ORF Insert Sequence:

ATGATCATCC ATTCGATCTT TTTGTTAATG GTATCAATCA CGCTCGTAAT TGCTGAAGAA 
TCTGACAATG TTTTATTCGA CAAACGTGCC CCTACGGGAC ATCAAGAAAT GCAAGGGAAA 
CAGAATTCTG CTTCTTTGAA CTCTGAAAAC TTTGGAATTT TCAAACGTGC GCTCATGGGT 
TTTCAGGGTG TGCGTGGAAA GAAAAACTCG ATAATCAATG ACGTCAAAAA TGAACTTTTT 
CCTGAGGACA TTAACAAGCG TGCACCGATG GGTTTTCAAG GCATGAGAGG GAAGAAGGCC 
TCGTTCGACG ATGAATATTA CAAACGTGCT CCGATGGGAT TTCAAGGAAT GAGAGGCAAA 
AAATCTCTTG AAGAGATTTT AGACGAGATT AAAAAGAAGA CTACGAGATT CCAGGATTCA 
AGAAGCAAAG ACGTGTACTT GATTGATTAT CCGGAAGATT ATGGAAAGAG AGTATTGTCG 
ATGGATGGAT ATCAAAATAT TCTCGATAAG AAGGACGAAT TACTGGGAGA GTGGGAAAAA 
AGAGCTCCGA TGGGATTTTA TGGAACGAGG GGTAAAAAAA TTATTCTTGA TGCGTTGGAG 
GAACTTGATA AACGAGGCGT TATGGATTTT CAAATAGGTT TGCAACGTAA GAAAGATACG 
ACGTTCGACG ATTATTTAGA TTATGCGATT AATCCGTTTG ATTACGAGAA AAGGAGTACG 
GATTTTCAAG ACGTAGAAAG CGGTAGCGAA TCCTTTAAAC GAGCTCGTAT GGGTTTTCAT 
GGGATGAGAG GTAAAAGGGA TGCGGCGGGG ATTTACGGAT CAAATTCAAG TACTGTGGGA 
ACGATTTTTG GTTATCAAGA TATGAGGAAT CGAGGAAATA ATTTTCCTGT ATATCAAGTT 
GAAAAACGAT CACCCTTCAG ATACCTTGGT GCCCGAGGTA AAAAGAATCC ACGATGGGAA 
TTTCGAGGGA AATTCGTAGG AGTGAGAGGC AAAAAATCGT CGTTGCAAAC TGTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tk ( XM_026441578.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026297363.1 Apis mellifera tachykinin (Tk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHd32052
Clone ID Related Accession (Same CDS sequence)	XM_026441578.1
Accession Version	XM_026441578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	tachykinins isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037644.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCATCC ATTCGATCTT TTTGTTAATG GTATCAATCA CGCTCGTAAT TGCTGAAGAA 
TCTGACAATG TTTTATTCGA CAAACGTGCC CCTACGGGAC ATCAAGAAAT GCAAGGGAAA 
CAGAATTCTG CTTCTTTGAA CTCTGAAAAC TTTGGAATTT TCAAACGTGC GCTCATGGGT 
TTTCAGGGTG TGCGTGGAAA GAAAAACTCG ATAATCAATG ACGTCAAAAA TGAACTTTTT 
CCTGAGGACA TTAACAAGCG TGCACCGATG GGTTTTCAAG GCATGAGAGG GAAGAAGGCC 
TCGTTCGACG ATGAATATTA CAAACGTGCT CCGATGGGAT TTCAAGGAAT GAGAGGCAAA 
AAATCTCTTG AAGAGATTTT AGACGAGATT AAAAAGAAGA CTACGAGATT CCAGGATTCA 
AGAAGCAAAG ACGTGTACTT GATTGATTAT CCGGAAGATT ATGGAAAGAG AGTATTGTCG 
ATGGATGGAT ATCAAAATAT TCTCGATAAG AAGGACGAAT TACTGGGAGA GTGGGAAAAA 
AGAGCTCCGA TGGGATTTTA TGGAACGAGG GGTAAAAAAA TTATTCTTGA TGCGTTGGAG 
GAACTTGATA AACGAGGCGT TATGGATTTT CAAATAGGTT TGCAACGTAA GAAAGATACG 
ACGTTCGACG ATTATTTAGA TTATGCGATT AATCCGTTTG ATTACGAGAA AAGGAGTACG 
GATTTTCAAG ACGTAGAAAG CGGTAGCGAA TCCTTTAAAC GAGCTCGTAT GGGTTTTCAT 
GGGATGAGAG GTAAAAGGGA TGCGGCGGGG ATTTACGGAT CAAATTCAAG TACTGTGGGA 
ACGATTTTTG GTTATCAAGA TATGAGGAAT CGAGGAAGTA ATTTTCCTGT ATATCAAGTT 
GAAAAACGAT CACCCTTCAG ATACCTTGGT GCCCGAGGTA AAAAGAATCC ACGATGGGAA 
TTTCGAGGGA AATTCGTAGG AGTGAGAGGC AAAAAATCGT CGTTGCAAAC TGTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026297363.1
CDS	25..1044
Translation	MIIHSIFLLMVSITLVIAEESDNVLFDKRAPTGHQEMQGKQNSASLNSENFGIFKRALMGFQGVRGKKNSIINDVKNELFPEDINKRAPMGFQGMRGKKASFDDEYYKRAPMGFQGMRGKKSLEEILDEIKKKTTRFQDSRSKDVYLIDYPEDYGKRVLSMDGYQNILDKKDELLGEWEKRAPMGFYGTRGKKIILDALEELDKRGVMDFQIGLQRKKDTTFDDYLDYAINPFDYEKRSTDFQDVESGSESFKRARMGFHGMRGKRDAAGIYGSNSSTVGTIFGYQDMRNRGSNFPVYQVEKRSPFRYLGARGKKNPRWEFRGKFVGVRGKKSSLQTVF

Target ORF information:

RefSeq Version	XM_026441578.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera tachykinin (Tk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026441578.1

ORF Insert Sequence:

ATGATCATCC ATTCGATCTT TTTGTTAATG GTATCAATCA CGCTCGTAAT TGCTGAAGAA 
TCTGACAATG TTTTATTCGA CAAACGTGCC CCTACGGGAC ATCAAGAAAT GCAAGGGAAA 
CAGAATTCTG CTTCTTTGAA CTCTGAAAAC TTTGGAATTT TCAAACGTGC GCTCATGGGT 
TTTCAGGGTG TGCGTGGAAA GAAAAACTCG ATAATCAATG ACGTCAAAAA TGAACTTTTT 
CCTGAGGACA TTAACAAGCG TGCACCGATG GGTTTTCAAG GCATGAGAGG GAAGAAGGCC 
TCGTTCGACG ATGAATATTA CAAACGTGCT CCGATGGGAT TTCAAGGAAT GAGAGGCAAA 
AAATCTCTTG AAGAGATTTT AGACGAGATT AAAAAGAAGA CTACGAGATT CCAGGATTCA 
AGAAGCAAAG ACGTGTACTT GATTGATTAT CCGGAAGATT ATGGAAAGAG AGTATTGTCG 
ATGGATGGAT ATCAAAATAT TCTCGATAAG AAGGACGAAT TACTGGGAGA GTGGGAAAAA 
AGAGCTCCGA TGGGATTTTA TGGAACGAGG GGTAAAAAAA TTATTCTTGA TGCGTTGGAG 
GAACTTGATA AACGAGGCGT TATGGATTTT CAAATAGGTT TGCAACGTAA GAAAGATACG 
ACGTTCGACG ATTATTTAGA TTATGCGATT AATCCGTTTG ATTACGAGAA AAGGAGTACG 
GATTTTCAAG ACGTAGAAAG CGGTAGCGAA TCCTTTAAAC GAGCTCGTAT GGGTTTTCAT 
GGGATGAGAG GTAAAAGGGA TGCGGCGGGG ATTTACGGAT CAAATTCAAG TACTGTGGGA 
ACGATTTTTG GTTATCAAGA TATGAGGAAT CGAGGAAGTA ATTTTCCTGT ATATCAAGTT 
GAAAAACGAT CACCCTTCAG ATACCTTGGT GCCCGAGGTA AAAAGAATCC ACGATGGGAA 
TTTCGAGGGA AATTCGTAGG AGTGAGAGGC AAAAAATCGT CGTTGCAAAC TGTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

From the genome to the proteome: uncovering peptides in the Apis brain.
Science (New York, N.Y.)314(5799)647-9(2006 Oct)
Hummon AB,Richmond TA,Verleyen P,Baggerman G,Huybrechts J,Ewing MA,Vierstraete E,Rodriguez-Zas SL,Schoofs L,Robinson GE,Sweedler JV

Prepro-tachykinin gene expression in the brain of the honeybee Apis mellifera.
Cell and tissue research316(2)281-93(2004 May)
Takeuchi H,Yasuda A,Yasuda-Kamatani Y,Sawata M,Matsuo Y,Kato A,Tsujimoto A,Nakajima T,Kubo T

Identification of a tachykinin-related neuropeptide from the honeybee brain using direct MALDI-TOF MS and its gene expression in worker, queen and drone heads.
Insect molecular biology12(3)291-8(2003 Jun)
Takeuchi H,Yasuda A,Yasuda-Kamatani Y,Kubo T,Nakajima T

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Tk cDNA ORF clone, Apis mellifera(honey bee)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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