SMG1 cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMG1
Entrez Gene ID	100477904
Full Name	SMG1, nonsense mediated mRNA decay associated PI3K related kinase
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011226514.2	XP_011224816.1	serine/threonine-protein kinase SMG1 isoform X1
XM_011226515.2	XP_011224817.1	serine/threonine-protein kinase SMG1 isoform X2

Pathways from BioSystems
Gene Ontology

The following SMG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc28414	XM_011226514.2 Latest version!	Ailuropoda melanoleuca SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAc28415	XM_011226515.2 Latest version!	Ailuropoda melanoleuca SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMG1 ( XM_011226514.2 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_011224816.1 Ailuropoda melanoleuca SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc28414
Clone ID Related Accession (Same CDS sequence)	XM_011226514.2
Accession Version	XM_011226514.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482768000000
Organism	Ailuropoda melanoleuca(giant panda)
Product	serine/threonine-protein kinase SMG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217710.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_011226514.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATACATTAAA ATATTCTTCA 
TCCAGAGATC GGGGTGGGTC TTCTTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACACTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAATAATGT GACCAGCCCT GAGTTCACCA GTATTCAGCA TGGCAGTCGT 
GCGTTAGCCA TCAAAGACAT GAGGAAATCA CAGGAGCGAT CGATGTCTTA TTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ACGACAGAGA CCGAAGACTG 
GCTACTGTAA AGCAGTTGAA AGAATTTATT CAGCAACCAG AAAATAAGCT GGTACTAGTT 
AAACAATTGG ATAATATCTT GGCTGCTGTA CATGATGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAGT TGAGACAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTCTGAGC 
TATGAGGCTG AGAAGATCTT CAAATGGATT TTTAGCAAAT TTAGCTCATC TGCAAAAGAT 
GAAGTTAAAC TCCTCTACTT ATGTGCCACC TACAAAGCAT TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCGGTAAT GCAGCTTGTG ATGACCAGCC TACAGTCCAT TCTTGAAAAT 
GTGGATACAC CAGAATTACT TTGCAAATGT GTTAAGTGCA TTCTATTGGT GGCTCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAT 
ATAGATCATA CTCAGAAACC TTCGCTCACA CAGCAGGTGT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA TCTTGCATTT TCTACCACTC TTCTTGGTCA GTTTCTAGAG 
GACATGGAGG CATATGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAGTC AGTGGATGAA 
GATGTTCCTC CTCCATCAGT GTCGCTACCA AAGCTGGCCG CGCTCCTTCG AGTGTTCAGT 
ACTGTAGTGA GGAGCATCGG GGAGCGCTTC AGCCCAATTC GGGGTCCTCC AATTACTGAG 
GCATATGTAA CAGATGTTCT ATACAGAGTA ATGAGATGTG TGACGGCTGC AAACCAAGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGATCAACTG 
GAGAACTGCC AGACTTGCGG TACAGATTAT ATCATCTCAG TCTTGAATTT ACTCACATTG 
ATTGTTGAAC AGATAAATAC AAAACTGCCA TCATCATTTG TAGAAAAACT GTTCATACCA 
TCATCTAAAC TATTATTTTT GCGTTACCAT AAAGAGAAGG AGGTTGTTGC TGTAGCCCAT 
GCTGTTTATC AAGCAGTGCT CAGCCTGAAG AATATTCCTG TTTTGGAGAC TGCCTATAAG 
TTAATTTTGG GGGAAATGAC TTGTGCCCTA AACAACCTGC TACATAGTCT ACAACTTCCT 
GATGCCTGCT CTGAAATAAA ACATGAGGCT TTTAAAAATC ACGTATTCAA TGTAGACAAT 
GCAAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCACTAATTG GGATGTGGGC GCTATCTCCA ACTGTCTTTG CACTGCTGAG TAAGAATCTG 
ATGATTGTGC ATGGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTACGC CGTGCTCTAC 
ACGTTGTATT CTCATTGTAC CAGGCACGAT CACTTTATAT CTAGTAGTCT TAGTTCTTCG 
TCCCCATCTT TGTTTGATGG AGCTGTGATT AGTACTGTAA CTACAGCTAC AAAAAAACAT 
TTCTCAATTA TACTGAATCT CCTGGGAATA CTGCTTAAGA AAGATAATCT TAACCAGGAT 
ACAAGGAAAC TGTTAATGAC CTGGGCTTTG GAAGTGGCTG TTTTAATGAA GAAGTCTGAA 
ACATATGCAC CTTTATTCTC TCTTCCATCT TTCCATAAAT TTTGCAAAGG CCTTTTAGCC 
AACACTCTTG TTGAAGATGT GAATATCTGT CTGCAAGCAT GCAGCAGTCT ACATGCTTTG 
TCTTCTTCGT TGCCAGATGA TCTTTTACAG AGGTGTGTCG ATGTTTGCCG TGTTCAACTT 
GTCCATAGTG GAACTCGTAT TCGACAAGCA TTTGGAAAGC TGTTGAAGTC AATTCCTTTA 
GATGTTGTTT TGAGCAATAA CAATCACACA GAAATTCAAG AGATTTCTTT AGCATTAAGA 
AGTCACATGA GCAAAGCACC AAGTAACACA TTCCACCCAC AAGATTTCTC TGATGTTATT 
AGTTTTATTT TGTATGGGAA TTCTCACAGA ACAGGGAAGG ACAATTGGTT AGAAAGATTG 
TTCTATAGCT GCCAGAGACT GGATAAGCGT GACCAATCAA CAATTCCCCG GAATCTTCTA 
AAGACAGATG CTGTCCTTTG GCAGTGGGCT ATTTGGGAGG CAGCACAGTT CACTGTCCTC 
TCTAAGTTGA GAACACCATT GGGCAGAGCT CAGGATACCT TCCAGACCAT TGAAGGTATC 
ATTAGAAGTC TTGCAGCTCA CACGTTAAAC CCCGATCAAG ATGTTAGTCA GTGGACAACT 
GCAGACAATG ATGAAGGCCA CAGTAGCAAC CAGCTTAGAC TTGTTCTTCT TCTGCAATAT 
CTGGAAAATC TGGAGAAGTT AATGTATAAT GCATATGAAG GGTGTGCTAA TGCATTAACA 
TCACCTCCCA AGGTCATTAG GACTTTTTTT TATACCAATC GCCAAACTTG CCAAGACTGG 
CTGACGCGGA TTCGACTCTC CATTATGAGG GTTGGGTTGT TGGCAGGCCA GCCCGCTGTG 
ACAGTGAGAC ATGGCTTTGA CTTGCTTACA GAAATGAAGA CAAATCTATC TCAGGGGAAT 
GAATTGGAAG TAACCATCAT GATGGTGGTA GAAGCACTGT GTGAGCTTCA TTGTCCTGAA 
GCTATACAGG GAATTGCTGT CTGGTCGTCT TCCATCGTTG GAAAGAATCT TCTCTGGATT 
AATTCTGTGG CCCAACAGGC TGAAGGGAGG TTTGAAAAGG CCTCTGTGGA GTACCAGGAG 
CACTTGTGCG CCATGACAGG GGTCGATTGC TGCATCTCCA GCTTTGACAA ATCTGTTCTC 
ACATTGGCCA ACAGTGGGCG TAACAGTGCC AGCCCCAAAC ATTCTCTGAA CGGTGAATCC 
AGAAAAACTG TGCTGTCCAA GCCAACTGAC TCTTCTCCTG AGGTTATAAA TTATTTGGGA 
AATAAAGCAT GTGAGTGCTA CATCTCCATT GCCGACTGGG CTGCGGTACA GGAGTGGCAG 
AATGCCATCC ACGACTTGAA GAAGAGTACC AGCAGTACTT CTCTCAACCT GAAAGCTGAT 
TTCAACTACA TTAAATCACT AAGCAGTTTT GAATCTGGAG AATTTGTTGA ATGTACTGAA 
CAATTAGAAT TGTTACCAGG AGAAAATATC AATCTACTTG CTGGAGGATC AAAAGAGAAA 
ATAGATATGA AAAAACTGCT TCCTAACATG TTAAGTCCGG ATCCAAGGGA ACTTCAGAAA 
TCCATTGAAG TTCAGTTGTT GAGAAGTTCT GTTTGTTTGG CAACTGCGTT AAACCATATA 
GAACAAGATC AGAAGTGGCA GTCCATAACT GAAAATGTGG TAAAGTACTT GAAGCAAACC 
TCCCGCATAG CTATTGGACC ACTGAGACTT TCTACTTTAA CAGTTTCACA GTCTCTGCCA 
GTTCTAAGTA CCTTACAGCT GTATTGCTCT TCTGCTTTGG AGAACACAGT TTCTAACAGA 
CTTTCAACAG AGGATTGTCT TATTCCACTC TTCAGTGAAG CTCTGCGTTC ATGTAAACAG 
CATGATGTGA GGCCATGGAT GCAGGCATTA AGATATACCA TGTACCAGAA TCAGTTGTTG 
GAGAAGATAA AAGAACAAAC AGTTCCAATT AGAAGCCATC TCATGGAATT AGGACTAACA 
GCAGCAAAAT TTGCTAGAAA ACGAGGGAAT GTGTCACTTG CAACAAGACT GCTGGCACAG 
TGTAGTGAGG TTCAGCTGGG AAAGACTACT ACTGCACAGG ATTTAGTCCA GCATTTTAAA 
AAACTATCAA CTCAAGGTCA AGTGGATGAA AAATGGGGGC CTGAACTTGA CATTGAAAAA 
ACCAAATTGC TATATACAGC AGGCCAGTCA ACACATGCAA TGGAAATGTT GAGTTCTTGT 
GCCATATCTT TCTGCAAGTC TGCCAAAGCC GAATATGCAG TTGCCAAGTC AATTCTGACA 
CTGGCTAAAT GGATCCAGGC CGAATGGAAA GAAATTTCAG GGCAGCTGAA ACAGGTTTAC 
AGAGCTCAGC AACAGCAGAA CTTCACAGGT CTTTCTACTC TGTCTAAAAA CATACTTGCT 
TTAATGGAAC TGCCATCTGT TAATGCAATG GAAGAAGAGT ATCCTCGGAT TGAGAGCGAA 
TCTACAGTAC ATATTGGAGT TGGAGAACCT GACTTCATTT TGGGACAGTT GTATCACCTG 
TCTTCAGTAC AGGCACCTGA AGTAGCCAAA TCCTGGGCAG CATTGGCTAG TTGGGCTTAT 
AGGTGGGGCA GAAAGGTGGT TGATAATGCC AGTCAGGGAG AAGGTGTTCG TCTGCTGCCT 
AGAGAAAAAT CTGAAGTTCA GAATCTGCTT CCAGACACTA TAACTGAAGA AGAAAAAGAG 
AGAATATATG GTATTCTTGG ACAGGCTGTG TGTCGGCCAG CAGGGATTCA GGATGAGGAC 
ATAACGCTTC AGATAACAGA AAGTGAAGAT AATGAGGAAG ATGACATGGT TGATGTTATC 
TGGCGTCAGT TAATCTCAAG CTGCCCGTGG CTTTCAGAAC TTGATGAAGG TGCAACAGAA 
GGAGTTATTA AAGTGTGGAG AAAGGTTGTA GATAGAATCT TCAGCCTGTA CAAGCTGTCT 
TGCAGTGCAT ATTTTACTTT CCTTAAACTC AATGCCGGTC AGATTCCTTT AGATGAAGAT 
GACCCTAGGC TACATTTAAG TCACAGAGCA GAGCAGAGTA CTGATGATGT GATTGTGATG 
GCAACACTGC GCTTGCTCAG ATTGCTCGTC AAACATGCCG GCGAGCTTAG GCAGTATCTG 
GAACATGGCT TGGAAACAAC ACCTACTGCT CCATGGAGAG GAATTATCCC ACAGCTTTTC 
TCACGCTTAA ACCATCCTGA AGTATATGTG CGCCAAAGTA TTTGTAACCT TCTCTGCCGA 
GTGGCTCAAG ATTCCCCGCA TCTCATATTG TACCCTGCGA TTGTGGGCAC TATATCACTT 
AGTACTGAAT CGCAGGCTTC AGGAAATAAG TTTTCGTCTG CAATTCCAAC TTTACTTGGC 
AACATTCAAG GAGAAGAGTT GCTGGTTTCT GAATGTGAGG GAGGAAGCCC TCCTGCTTCT 
CAGGATAGCA ATAAGGATGA ACCTAAAAGT GGATTAAATG AAGACCAAGC CATGATGCAG 
GATTGCTACA GCAAAATTGT AGATAAGCTG TCCTCTGCAA ACCCAACTAT GGTGTTACAG 
GTCCAGATGC TTGTGGCTGA GCTGCGCAGG GTCACTGTTC TCTGGGATGA GCTCTGGTTG 
GGAGTTCTGT TACAGCAACA CATGTATGTC CTGAGACGGA TCCAGCAGCT GGAAGATGAG 
GTGAAGAGAG TCCAGAACAA CAACACCTTG CGGAAGGAAG AGAAAATTGC AATCATGCGT 
GAAAAGCACA CGGCTTTGAT GAAGCCCATT GTGTTTGCTT TGGAGCACGT AAGGAGCATC 
ACTGCAGCCC CTGCAGAAAC ACCTCATGAA AAGTGGTTTC AGGATAACTA TGGTGAAGCA 
ATTGAAAATG CTCTTGAAAA GCTGAAGACA CCATCAAATC CTGCAAAGCC TGGAAGCAGC 
TGGATTCCAT TTAAAGAGAT AATGCTGAGT TTGCAACAGA GAGCACAGAA ACGTGCAAGT 
TATATCTTGC GTCTTGAAGA AATCAGCCCA TGGCTGGCTG CCATGACTAA CACTGAAATT 
GCTCTTCCTG GAGAAGTGTC AGCTAGAGAC ACTGTCACAA TCCACAGTGT GGGTGGAACT 
ATCACCATCT TACCTACCAA AACCAAGCCA AAGAAACTTC TCTTTCTAGG GTCAGATGGC 
AAGAGCTATC CTTATCTTTT CAAAGGATTA GAAGATTTAC ATCTGGATGA GAGAATAATG 
CAATTCCTGT CTATTGTGAA TACCATGTTT GCTACAATCA ATCGCCAGGA AACACCCCGT 
TTCCACGCCC GACACTATTC CGTAACGCCA CTAGGAACAC GATCAGGACT AATCCAGTGG 
GTGGATGGAG CCACACCCTT ATTTGGTCTT TACAAACGAT GGCAACAACG GGAAGCTGCC 
TTACAGGCAC AGAAGGCCCA AGATTCCTAC CAAACTCCTC AGAATCCTGG AATTGTACCT 
CGTCCTAGTG AACTTTATTA TAGTAAAATT GGCCCTGCTT TAAAAGCAGT TGGACTTAGT 
CTCGATGTGT CCCGACGGGA TTGGCCTCTT CATGTAATGA AGGCGGTATT AGAAGAGTTA 
ATGGAGGCCA CCCCCCCAAA TCTCCTTGCT AAAGAGCTTT GGTCCTCTTG CACCACACCT 
GATGAATGGT GGAGAGTCAC ACAGTCTTAT GCCAGATCTA CTGCAGTCAT GTCTATGGTT 
GGATACATAA TTGGCCTTGG AGATAGACAT CTGGATAATG TTCTCATAGA TATGACGACT 
GGAGAGGTGG TTCACATAGA TTACAATGTT TGCTTTGAAA AAGGTAAAAG CCTTAGAGTT 
CCTGAGAAAG TACCTTTTCG AATGACACAG AATATTGAAA CAGCACTGGG TGTAACTGGA 
GTAGAAGGTG TTTTTAGGCT TTCATGTGAG CAGGTTCTAC ACATCATGCG GCGTGGCAGA 
GAGACTCTGC TGACATTGCT GGAGGCCTTT GTGTATGACC CCCTGGTGGA CTGGACGGCC 
GGGGGCGAGG CGGGATTTGC CGGCGCTGTC TATGGTGGAG GTGGCCAGCA GGCTGAGAGC 
AAGCAGAGCA AGAGAGAGAT GGAGCGAGAG ATCACCCGTA GCCTCTTTTC TTCCAGAGTA 
GCTGAAATTA AGGTGAACTG GTTTAAGAAT AGGGATGAGA TGCAGGTCGT GCTTCCCAAG 
CTGGACAGCA GCTTAGATGA ATACCTAAGC TTGCAAGAGC AGCTGACAGA TGTGGAAAAA 
CTTCAAGGCA AACTGCTGGA AGAAATAGAG TTTTTAGAAG GAGCTGAAGG GGTGGACCAT 
CCTTCTCACA CTCTGCAACA CAGGTATTCT GAACACACTC AACTACAGAC TCAGCAAAGG 
GCTGTTCAGG AAGCAATCCA GGTGAAGCTG AATGAATTTG AACAGTGGAT AACACATTAT 
CAGGCTGCGT TCAATAATTT AGAAGCAACA CAGCTTGCAA GTCTGCTTCA GGAGATAAGC 
TCACAAATGG ACCTTGGTCC TCCAAGCTAT GTGCCAGCGA CAGCCTTTCT ACAGAATGCT 
GGCCAGGCGC ACTTGATTAG CCAGTGTGAG CAGCTGGAGG GGGAAGTGGG CGCTCTTCTC 
CAGCAGAGGC GTTCGGTGCT CCGAGGTTGC CTGGAACAGC TGCATCACTA TGCAACCGTA 
GCTCTGCAGT ATCCAAAGGC CATATTTCAG AAACATCGAA TTGAGCAGTG GAAGACCTGG 
ATGGAAGAAC TCATCTGTAA CACCACAGTA GAGCGCTGTC AAGAACTCTA CAGGAAATAT 
GAGATGCAGT ATGCTCCCCA GCCTCCACCA GCAGTGTGTC AGTTCATCAC TGCCACGGAG 
ATGACCCTGC AGCGGTACGC AGCTGACATC AACAGCAGGC TGATCAGACA AGTGGAACGC 
TTGAAACAGG AAGCTGTCAC TGTGCCTGTT TGTGAAGATC AGTTGAAAGA AATTGAACGT 
TGCATTAAAG TTTTCCTTCA CGAGAATGGA GAAGAAGGAT CTTTGAGTCT AGCAAGTGTT 
ATTATTTCTG CCCTTTGTAC TCTTACAAGG CGGAACCTGA TGATGGAAGG TGCAGCTTCA 
AGTGCTGGGG AGCAGCTGGT GGACCTGACC TCTCGGGATG GAGCCTGGTT CTTGGAAGAG 
CTCTGCAGTA TGAGCGGGAA CGTCACTTGC CTGGTGCAGT TGCTGAAGCA GTGCCACCTG 
GTGCCACAGG ACTTGGACAT TCCGAACCCA ATGGAAGCTT CTGAGGCAGT TCATTTAGCT 
AATGGAGTGT ATACCTCACT TCAGGAATTA AATTCAAATT TCCGGCAAAT CATATTTCCA 
GAAGCACTGA GATGTTTAAT GAAAGGAGAA TACACATTAG AAAGTATGCT TCATGAGCTG 
GACAGTCTTA TTGAGCAGAC AACTGACGGC GTTCCCCTGC AGACTCTAGT AGAATCTCTT 
CAGGCCTATT TAAGAAATGC GGCTATGGGA CTCGAGGAGG AAACGCATGC TCATTACATC 
GATGTTGCCA GAATACTTCA TGCTCAATAT GGTGAATTAA TCCAACCAAG AAACGGTTCG 
GTTGATGAAA CACCAAAAAT GTCAGCTGGC CAGATGCTTT TGGTAGCATT TGATGGCATG 
TTTGCCCAAG TTGAAACTGC TTTTGGCTTA TTAGTTGAAA AGTTAAATAA GATGGAAATT 
CCCGTAGCTT GGCGAAAGAT TGACATTATA AGGGAAGCCA GGAGTACCCA AGTCAATTTT 
TTTGATGACG ATAATCACCG GCAGGTGCTA GAGGAGATTT TCTTTCTAAA AAGACTACAG 
ACAATTAAGG AGTTCTTCAG GCTCTGTGGT ACCTTTTCTA AAACTTTATC AGGATCGAGT 
TCACTTGAAG ATCAGAATAC TGTAAATGGA CCTGTACAGA TTGTCAACGT GAAAACCCTT 
TTTAGAAACT CCTGTTTCAG TGAAGACCAG ATGGCCAAAC CCATAAAGGC TTTCACAGCT 
GACTTCGTGA GGCAGCTCTT GATTGGACTC CCGAACCAAG CCCTAGGACT CACCCTCTGC 
AGTTTTATCA GTGCTCTAGG GGTAGACATT ATTGCTCAAG TAGAGGCAAA GGATTTTGGT 
GCTGAAAGCA AAGTTTCTGT TGATGATCTT TGTAAGAAAG CAGTGGAGCA CAACATTCAG 
ATTGGGAAGT TCTCTCAGCT GGTCATGAAC AGGGCAACTG TGTTAGCAAG TTCCTATGAT 
ACTGCCTGGA AGAAGCATGA CTTGGTGCGC AGGCTGGAAA CCAGTATTTC TTCTTGTAAG 
ACAAGCCTGC AGCGTGTTCA GCTGCATATT GCCATGTTTC AATGGCAGCA TGAAGACCTC 
CTTATCAATA GACCACAAGC CATGTCAGTC ACACCTCCCC CTCGGTCCGC CATCCTAACC 
AGCATGAAAA AGAAACTCCA TACTCTGAGC CAAATTGAAA CTTCAATTGC AACAGTTCAG 
GAGAAACTAG CAGCCCTTGA AGCAAGTATT GAACAGCGAC TCAAGTGGGC AGGTGGTGCC 
AACCCTGCAC TGGCACCTGT GTTGCAGGAT TTTGAAGCAA CAATAGCTGA AAGAAGAAAT 
CTTGTCCTTA AAGAGAGCCA AAGAGCAAGC CAGGTCACTT TCCTCTGCAG CAATATCATT 
CATTTTGAAA GTTTACGAAC TAGGACTGCA GAAGCCTTAA ACCTGGATGC TGCATTATTT 
GAACTAATCA AACGATGTCA GCAAATGTGT TCATTTGCAT CACAGTTTAA CAGTTCAGTC 
TCTGAGTTAG AGCTTCGTCT GTTACAGAGA GTGGACACCA GTCTTGAACA TCCTATTGGC 
AGCTCTGAAT GGCTTTTGTC AGCACACAAA CAATTGACCC AGGATATGTC TACTCAGAGA 
GCAATTCAGA CAGAGAAGGA GCAACAGATA GAAACAGTCT GTGAAACAAT TCAGAATCTG 
GTTGATAATA TAAAGACTGT GCTCACTGGT CATAACCGGC AACTTGGAGA TGTCAAACAT 
CTCCTGAAAG CAATGGCTAA GGATGAAGAA GCTGCTCTGG CAGATGGTGA AGATGTTCCC 
TATGAGAACA GTGTTAGGCA ATTCTTAGGT GAATATAAAT CCTGGCAAGA CAACATTCAG 
ACGGTGCTCT TTACGTTAGT CCAAGCTATG GGTCAGGTTC GAAGTCAAGA ACACATTGAA 
ATGCTCCAGG AAATAACTCC CACCTTGAAA GAACTGAAAA CACAAAGTCA GAGTATCTAT 
AATAATTTAG TGGGTTTTGC ATCACCCTTA GTCACCGATG CAACAAATGA ATGTTCGAGT 
CCAACGTCAT CTGCTACTTA TCAGCCATCC TTTGCTGCAG CAGTCCGGAG CAACACTGGC 
CAGAAGACTC AGCCTGATGT CATGTCACAG AATGCTAGAA AGCTGATTCA GAAAAATCTT 
GCCACATCAG CAGATACTCC ACCAAGCACC ATTCCAGGAA CTGGCAAGAG TGTTGCTTGT 
AGTCCTAAAA AGGCTGTCAG AGACCCTAAA ACAGGGAAAG CTGTGCAAGA GAGAAACTCC 
TATGCAGTGA GTGTGTGGAA GAGGGTGAAA GCCAAGCTAG AGGGCCGCGA TGTTGATCCT 
AATAGGAGAA TGTCAGTTGC TGAACAGGTT GACTATGTCA TTAAGGAAGC AACTAATCTA 
GATAACTTGG CTCAGCTGTA TGAAGGTTGG ACAGCCTGGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011224816.1
CDS	83..10987
Translation	MRFLRTDSASADPDTLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHTDIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSIQHGSRALAIKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPDACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANSGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNHIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNFTGLSTLSKNILALMELPSVNAMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDEGATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRAEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSTESQASGNKFSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGEAIENALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMQVVLPKLDSSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISSQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPAVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDSLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARILHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPVAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTSLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHIEMLQEITPTLKELKTQSQSIYNNLVGFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTIPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_011226514.2
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011226514.2

ORF Insert Sequence:

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATACATTAAA ATATTCTTCA 
TCCAGAGATC GGGGTGGGTC TTCTTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACACTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAATAATGT GACCAGCCCT GAGTTCACCA GTATTCAGCA TGGCAGTCGT 
GCGTTAGCCA TCAAAGACAT GAGGAAATCA CAGGAGCGAT CGATGTCTTA TTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ACGACAGAGA CCGAAGACTG 
GCTACTGTAA AGCAGTTGAA AGAATTTATT CAGCAACCAG AAAATAAGCT GGTACTAGTT 
AAACAATTGG ATAATATCTT GGCTGCTGTA CATGATGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAGT TGAGACAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTCTGAGC 
TATGAGGCTG AGAAGATCTT CAAATGGATT TTTAGCAAAT TTAGCTCATC TGCAAAAGAT 
GAAGTTAAAC TCCTCTACTT ATGTGCCACC TACAAAGCAT TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCGGTAAT GCAGCTTGTG ATGACCAGCC TACAGTCCAT TCTTGAAAAT 
GTGGATACAC CAGAATTACT TTGCAAATGT GTTAAGTGCA TTCTATTGGT GGCTCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAT 
ATAGATCATA CTCAGAAACC TTCGCTCACA CAGCAGGTGT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA TCTTGCATTT TCTACCACTC TTCTTGGTCA GTTTCTAGAG 
GACATGGAGG CATATGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAGTC AGTGGATGAA 
GATGTTCCTC CTCCATCAGT GTCGCTACCA AAGCTGGCCG CGCTCCTTCG AGTGTTCAGT 
ACTGTAGTGA GGAGCATCGG GGAGCGCTTC AGCCCAATTC GGGGTCCTCC AATTACTGAG 
GCATATGTAA CAGATGTTCT ATACAGAGTA ATGAGATGTG TGACGGCTGC AAACCAAGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGATCAACTG 
GAGAACTGCC AGACTTGCGG TACAGATTAT ATCATCTCAG TCTTGAATTT ACTCACATTG 
ATTGTTGAAC AGATAAATAC AAAACTGCCA TCATCATTTG TAGAAAAACT GTTCATACCA 
TCATCTAAAC TATTATTTTT GCGTTACCAT AAAGAGAAGG AGGTTGTTGC TGTAGCCCAT 
GCTGTTTATC AAGCAGTGCT CAGCCTGAAG AATATTCCTG TTTTGGAGAC TGCCTATAAG 
TTAATTTTGG GGGAAATGAC TTGTGCCCTA AACAACCTGC TACATAGTCT ACAACTTCCT 
GATGCCTGCT CTGAAATAAA ACATGAGGCT TTTAAAAATC ACGTATTCAA TGTAGACAAT 
GCAAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCACTAATTG GGATGTGGGC GCTATCTCCA ACTGTCTTTG CACTGCTGAG TAAGAATCTG 
ATGATTGTGC ATGGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTACGC CGTGCTCTAC 
ACGTTGTATT CTCATTGTAC CAGGCACGAT CACTTTATAT CTAGTAGTCT TAGTTCTTCG 
TCCCCATCTT TGTTTGATGG AGCTGTGATT AGTACTGTAA CTACAGCTAC AAAAAAACAT 
TTCTCAATTA TACTGAATCT CCTGGGAATA CTGCTTAAGA AAGATAATCT TAACCAGGAT 
ACAAGGAAAC TGTTAATGAC CTGGGCTTTG GAAGTGGCTG TTTTAATGAA GAAGTCTGAA 
ACATATGCAC CTTTATTCTC TCTTCCATCT TTCCATAAAT TTTGCAAAGG CCTTTTAGCC 
AACACTCTTG TTGAAGATGT GAATATCTGT CTGCAAGCAT GCAGCAGTCT ACATGCTTTG 
TCTTCTTCGT TGCCAGATGA TCTTTTACAG AGGTGTGTCG ATGTTTGCCG TGTTCAACTT 
GTCCATAGTG GAACTCGTAT TCGACAAGCA TTTGGAAAGC TGTTGAAGTC AATTCCTTTA 
GATGTTGTTT TGAGCAATAA CAATCACACA GAAATTCAAG AGATTTCTTT AGCATTAAGA 
AGTCACATGA GCAAAGCACC AAGTAACACA TTCCACCCAC AAGATTTCTC TGATGTTATT 
AGTTTTATTT TGTATGGGAA TTCTCACAGA ACAGGGAAGG ACAATTGGTT AGAAAGATTG 
TTCTATAGCT GCCAGAGACT GGATAAGCGT GACCAATCAA CAATTCCCCG GAATCTTCTA 
AAGACAGATG CTGTCCTTTG GCAGTGGGCT ATTTGGGAGG CAGCACAGTT CACTGTCCTC 
TCTAAGTTGA GAACACCATT GGGCAGAGCT CAGGATACCT TCCAGACCAT TGAAGGTATC 
ATTAGAAGTC TTGCAGCTCA CACGTTAAAC CCCGATCAAG ATGTTAGTCA GTGGACAACT 
GCAGACAATG ATGAAGGCCA CAGTAGCAAC CAGCTTAGAC TTGTTCTTCT TCTGCAATAT 
CTGGAAAATC TGGAGAAGTT AATGTATAAT GCATATGAAG GGTGTGCTAA TGCATTAACA 
TCACCTCCCA AGGTCATTAG GACTTTTTTT TATACCAATC GCCAAACTTG CCAAGACTGG 
CTGACGCGGA TTCGACTCTC CATTATGAGG GTTGGGTTGT TGGCAGGCCA GCCCGCTGTG 
ACAGTGAGAC ATGGCTTTGA CTTGCTTACA GAAATGAAGA CAAATCTATC TCAGGGGAAT 
GAATTGGAAG TAACCATCAT GATGGTGGTA GAAGCACTGT GTGAGCTTCA TTGTCCTGAA 
GCTATACAGG GAATTGCTGT CTGGTCGTCT TCCATCGTTG GAAAGAATCT TCTCTGGATT 
AATTCTGTGG CCCAACAGGC TGAAGGGAGG TTTGAAAAGG CCTCTGTGGA GTACCAGGAG 
CACTTGTGCG CCATGACAGG GGTCGATTGC TGCATCTCCA GCTTTGACAA ATCTGTTCTC 
ACATTGGCCA ACAGTGGGCG TAACAGTGCC AGCCCCAAAC ATTCTCTGAA CGGTGAATCC 
AGAAAAACTG TGCTGTCCAA GCCAACTGAC TCTTCTCCTG AGGTTATAAA TTATTTGGGA 
AATAAAGCAT GTGAGTGCTA CATCTCCATT GCCGACTGGG CTGCGGTACA GGAGTGGCAG 
AATGCCATCC ACGACTTGAA GAAGAGTACC AGCAGTACTT CTCTCAACCT GAAAGCTGAT 
TTCAACTACA TTAAATCACT AAGCAGTTTT GAATCTGGAG AATTTGTTGA ATGTACTGAA 
CAATTAGAAT TGTTACCAGG AGAAAATATC AATCTACTTG CTGGAGGATC AAAAGAGAAA 
ATAGATATGA AAAAACTGCT TCCTAACATG TTAAGTCCGG ATCCAAGGGA ACTTCAGAAA 
TCCATTGAAG TTCAGTTGTT GAGAAGTTCT GTTTGTTTGG CAACTGCGTT AAACCATATA 
GAACAAGATC AGAAGTGGCA GTCCATAACT GAAAATGTGG TAAAGTACTT GAAGCAAACC 
TCCCGCATAG CTATTGGACC ACTGAGACTT TCTACTTTAA CAGTTTCACA GTCTCTGCCA 
GTTCTAAGTA CCTTACAGCT GTATTGCTCT TCTGCTTTGG AGAACACAGT TTCTAACAGA 
CTTTCAACAG AGGATTGTCT TATTCCACTC TTCAGTGAAG CTCTGCGTTC ATGTAAACAG 
CATGATGTGA GGCCATGGAT GCAGGCATTA AGATATACCA TGTACCAGAA TCAGTTGTTG 
GAGAAGATAA AAGAACAAAC AGTTCCAATT AGAAGCCATC TCATGGAATT AGGACTAACA 
GCAGCAAAAT TTGCTAGAAA ACGAGGGAAT GTGTCACTTG CAACAAGACT GCTGGCACAG 
TGTAGTGAGG TTCAGCTGGG AAAGACTACT ACTGCACAGG ATTTAGTCCA GCATTTTAAA 
AAACTATCAA CTCAAGGTCA AGTGGATGAA AAATGGGGGC CTGAACTTGA CATTGAAAAA 
ACCAAATTGC TATATACAGC AGGCCAGTCA ACACATGCAA TGGAAATGTT GAGTTCTTGT 
GCCATATCTT TCTGCAAGTC TGCCAAAGCC GAATATGCAG TTGCCAAGTC AATTCTGACA 
CTGGCTAAAT GGATCCAGGC CGAATGGAAA GAAATTTCAG GGCAGCTGAA ACAGGTTTAC 
AGAGCTCAGC AACAGCAGAA CTTCACAGGT CTTTCTACTC TGTCTAAAAA CATACTTGCT 
TTAATGGAAC TGCCATCTGT TAATGCAATG GAAGAAGAGT ATCCTCGGAT TGAGAGCGAA 
TCTACAGTAC ATATTGGAGT TGGAGAACCT GACTTCATTT TGGGACAGTT GTATCACCTG 
TCTTCAGTAC AGGCACCTGA AGTAGCCAAA TCCTGGGCAG CATTGGCTAG TTGGGCTTAT 
AGGTGGGGCA GAAAGGTGGT TGATAATGCC AGTCAGGGAG AAGGTGTTCG TCTGCTGCCT 
AGAGAAAAAT CTGAAGTTCA GAATCTGCTT CCAGACACTA TAACTGAAGA AGAAAAAGAG 
AGAATATATG GTATTCTTGG ACAGGCTGTG TGTCGGCCAG CAGGGATTCA GGATGAGGAC 
ATAACGCTTC AGATAACAGA AAGTGAAGAT AATGAGGAAG ATGACATGGT TGATGTTATC 
TGGCGTCAGT TAATCTCAAG CTGCCCGTGG CTTTCAGAAC TTGATGAAGG TGCAACAGAA 
GGAGTTATTA AAGTGTGGAG AAAGGTTGTA GATAGAATCT TCAGCCTGTA CAAGCTGTCT 
TGCAGTGCAT ATTTTACTTT CCTTAAACTC AATGCCGGTC AGATTCCTTT AGATGAAGAT 
GACCCTAGGC TACATTTAAG TCACAGAGCA GAGCAGAGTA CTGATGATGT GATTGTGATG 
GCAACACTGC GCTTGCTCAG ATTGCTCGTC AAACATGCCG GCGAGCTTAG GCAGTATCTG 
GAACATGGCT TGGAAACAAC ACCTACTGCT CCATGGAGAG GAATTATCCC ACAGCTTTTC 
TCACGCTTAA ACCATCCTGA AGTATATGTG CGCCAAAGTA TTTGTAACCT TCTCTGCCGA 
GTGGCTCAAG ATTCCCCGCA TCTCATATTG TACCCTGCGA TTGTGGGCAC TATATCACTT 
AGTACTGAAT CGCAGGCTTC AGGAAATAAG TTTTCGTCTG CAATTCCAAC TTTACTTGGC 
AACATTCAAG GAGAAGAGTT GCTGGTTTCT GAATGTGAGG GAGGAAGCCC TCCTGCTTCT 
CAGGATAGCA ATAAGGATGA ACCTAAAAGT GGATTAAATG AAGACCAAGC CATGATGCAG 
GATTGCTACA GCAAAATTGT AGATAAGCTG TCCTCTGCAA ACCCAACTAT GGTGTTACAG 
GTCCAGATGC TTGTGGCTGA GCTGCGCAGG GTCACTGTTC TCTGGGATGA GCTCTGGTTG 
GGAGTTCTGT TACAGCAACA CATGTATGTC CTGAGACGGA TCCAGCAGCT GGAAGATGAG 
GTGAAGAGAG TCCAGAACAA CAACACCTTG CGGAAGGAAG AGAAAATTGC AATCATGCGT 
GAAAAGCACA CGGCTTTGAT GAAGCCCATT GTGTTTGCTT TGGAGCACGT AAGGAGCATC 
ACTGCAGCCC CTGCAGAAAC ACCTCATGAA AAGTGGTTTC AGGATAACTA TGGTGAAGCA 
ATTGAAAATG CTCTTGAAAA GCTGAAGACA CCATCAAATC CTGCAAAGCC TGGAAGCAGC 
TGGATTCCAT TTAAAGAGAT AATGCTGAGT TTGCAACAGA GAGCACAGAA ACGTGCAAGT 
TATATCTTGC GTCTTGAAGA AATCAGCCCA TGGCTGGCTG CCATGACTAA CACTGAAATT 
GCTCTTCCTG GAGAAGTGTC AGCTAGAGAC ACTGTCACAA TCCACAGTGT GGGTGGAACT 
ATCACCATCT TACCTACCAA AACCAAGCCA AAGAAACTTC TCTTTCTAGG GTCAGATGGC 
AAGAGCTATC CTTATCTTTT CAAAGGATTA GAAGATTTAC ATCTGGATGA GAGAATAATG 
CAATTCCTGT CTATTGTGAA TACCATGTTT GCTACAATCA ATCGCCAGGA AACACCCCGT 
TTCCACGCCC GACACTATTC CGTAACGCCA CTAGGAACAC GATCAGGACT AATCCAGTGG 
GTGGATGGAG CCACACCCTT ATTTGGTCTT TACAAACGAT GGCAACAACG GGAAGCTGCC 
TTACAGGCAC AGAAGGCCCA AGATTCCTAC CAAACTCCTC AGAATCCTGG AATTGTACCT 
CGTCCTAGTG AACTTTATTA TAGTAAAATT GGCCCTGCTT TAAAAGCAGT TGGACTTAGT 
CTCGATGTGT CCCGACGGGA TTGGCCTCTT CATGTAATGA AGGCGGTATT AGAAGAGTTA 
ATGGAGGCCA CCCCCCCAAA TCTCCTTGCT AAAGAGCTTT GGTCCTCTTG CACCACACCT 
GATGAATGGT GGAGAGTCAC ACAGTCTTAT GCCAGATCTA CTGCAGTCAT GTCTATGGTT 
GGATACATAA TTGGCCTTGG AGATAGACAT CTGGATAATG TTCTCATAGA TATGACGACT 
GGAGAGGTGG TTCACATAGA TTACAATGTT TGCTTTGAAA AAGGTAAAAG CCTTAGAGTT 
CCTGAGAAAG TACCTTTTCG AATGACACAG AATATTGAAA CAGCACTGGG TGTAACTGGA 
GTAGAAGGTG TTTTTAGGCT TTCATGTGAG CAGGTTCTAC ACATCATGCG GCGTGGCAGA 
GAGACTCTGC TGACATTGCT GGAGGCCTTT GTGTATGACC CCCTGGTGGA CTGGACGGCC 
GGGGGCGAGG CGGGATTTGC CGGCGCTGTC TATGGTGGAG GTGGCCAGCA GGCTGAGAGC 
AAGCAGAGCA AGAGAGAGAT GGAGCGAGAG ATCACCCGTA GCCTCTTTTC TTCCAGAGTA 
GCTGAAATTA AGGTGAACTG GTTTAAGAAT AGGGATGAGA TGCAGGTCGT GCTTCCCAAG 
CTGGACAGCA GCTTAGATGA ATACCTAAGC TTGCAAGAGC AGCTGACAGA TGTGGAAAAA 
CTTCAAGGCA AACTGCTGGA AGAAATAGAG TTTTTAGAAG GAGCTGAAGG GGTGGACCAT 
CCTTCTCACA CTCTGCAACA CAGGTATTCT GAACACACTC AACTACAGAC TCAGCAAAGG 
GCTGTTCAGG AAGCAATCCA GGTGAAGCTG AATGAATTTG AACAGTGGAT AACACATTAT 
CAGGCTGCGT TCAATAATTT AGAAGCAACA CAGCTTGCAA GTCTGCTTCA GGAGATAAGC 
TCACAAATGG ACCTTGGTCC TCCAAGCTAT GTGCCAGCGA CAGCCTTTCT ACAGAATGCT 
GGCCAGGCGC ACTTGATTAG CCAGTGTGAG CAGCTGGAGG GGGAAGTGGG CGCTCTTCTC 
CAGCAGAGGC GTTCGGTGCT CCGAGGTTGC CTGGAACAGC TGCATCACTA TGCAACCGTA 
GCTCTGCAGT ATCCAAAGGC CATATTTCAG AAACATCGAA TTGAGCAGTG GAAGACCTGG 
ATGGAAGAAC TCATCTGTAA CACCACAGTA GAGCGCTGTC AAGAACTCTA CAGGAAATAT 
GAGATGCAGT ATGCTCCCCA GCCTCCACCA GCAGTGTGTC AGTTCATCAC TGCCACGGAG 
ATGACCCTGC AGCGGTACGC AGCTGACATC AACAGCAGGC TGATCAGACA AGTGGAACGC 
TTGAAACAGG AAGCTGTCAC TGTGCCTGTT TGTGAAGATC AGTTGAAAGA AATTGAACGT 
TGCATTAAAG TTTTCCTTCA CGAGAATGGA GAAGAAGGAT CTTTGAGTCT AGCAAGTGTT 
ATTATTTCTG CCCTTTGTAC TCTTACAAGG CGGAACCTGA TGATGGAAGG TGCAGCTTCA 
AGTGCTGGGG AGCAGCTGGT GGACCTGACC TCTCGGGATG GAGCCTGGTT CTTGGAAGAG 
CTCTGCAGTA TGAGCGGGAA CGTCACTTGC CTGGTGCAGT TGCTGAAGCA GTGCCACCTG 
GTGCCACAGG ACTTGGACAT TCCGAACCCA ATGGAAGCTT CTGAGGCAGT TCATTTAGCT 
AATGGAGTGT ATACCTCACT TCAGGAATTA AATTCAAATT TCCGGCAAAT CATATTTCCA 
GAAGCACTGA GATGTTTAAT GAAAGGAGAA TACACATTAG AAAGTATGCT TCATGAGCTG 
GACAGTCTTA TTGAGCAGAC AACTGACGGC GTTCCCCTGC AGACTCTAGT AGAATCTCTT 
CAGGCCTATT TAAGAAATGC GGCTATGGGA CTCGAGGAGG AAACGCATGC TCATTACATC 
GATGTTGCCA GAATACTTCA TGCTCAATAT GGTGAATTAA TCCAACCAAG AAACGGTTCG 
GTTGATGAAA CACCAAAAAT GTCAGCTGGC CAGATGCTTT TGGTAGCATT TGATGGCATG 
TTTGCCCAAG TTGAAACTGC TTTTGGCTTA TTAGTTGAAA AGTTAAATAA GATGGAAATT 
CCCGTAGCTT GGCGAAAGAT TGACATTATA AGGGAAGCCA GGAGTACCCA AGTCAATTTT 
TTTGATGACG ATAATCACCG GCAGGTGCTA GAGGAGATTT TCTTTCTAAA AAGACTACAG 
ACAATTAAGG AGTTCTTCAG GCTCTGTGGT ACCTTTTCTA AAACTTTATC AGGATCGAGT 
TCACTTGAAG ATCAGAATAC TGTAAATGGA CCTGTACAGA TTGTCAACGT GAAAACCCTT 
TTTAGAAACT CCTGTTTCAG TGAAGACCAG ATGGCCAAAC CCATAAAGGC TTTCACAGCT 
GACTTCGTGA GGCAGCTCTT GATTGGACTC CCGAACCAAG CCCTAGGACT CACCCTCTGC 
AGTTTTATCA GTGCTCTAGG GGTAGACATT ATTGCTCAAG TAGAGGCAAA GGATTTTGGT 
GCTGAAAGCA AAGTTTCTGT TGATGATCTT TGTAAGAAAG CAGTGGAGCA CAACATTCAG 
ATTGGGAAGT TCTCTCAGCT GGTCATGAAC AGGGCAACTG TGTTAGCAAG TTCCTATGAT 
ACTGCCTGGA AGAAGCATGA CTTGGTGCGC AGGCTGGAAA CCAGTATTTC TTCTTGTAAG 
ACAAGCCTGC AGCGTGTTCA GCTGCATATT GCCATGTTTC AATGGCAGCA TGAAGACCTC 
CTTATCAATA GACCACAAGC CATGTCAGTC ACACCTCCCC CTCGGTCCGC CATCCTAACC 
AGCATGAAAA AGAAACTCCA TACTCTGAGC CAAATTGAAA CTTCAATTGC AACAGTTCAG 
GAGAAACTAG CAGCCCTTGA AGCAAGTATT GAACAGCGAC TCAAGTGGGC AGGTGGTGCC 
AACCCTGCAC TGGCACCTGT GTTGCAGGAT TTTGAAGCAA CAATAGCTGA AAGAAGAAAT 
CTTGTCCTTA AAGAGAGCCA AAGAGCAAGC CAGGTCACTT TCCTCTGCAG CAATATCATT 
CATTTTGAAA GTTTACGAAC TAGGACTGCA GAAGCCTTAA ACCTGGATGC TGCATTATTT 
GAACTAATCA AACGATGTCA GCAAATGTGT TCATTTGCAT CACAGTTTAA CAGTTCAGTC 
TCTGAGTTAG AGCTTCGTCT GTTACAGAGA GTGGACACCA GTCTTGAACA TCCTATTGGC 
AGCTCTGAAT GGCTTTTGTC AGCACACAAA CAATTGACCC AGGATATGTC TACTCAGAGA 
GCAATTCAGA CAGAGAAGGA GCAACAGATA GAAACAGTCT GTGAAACAAT TCAGAATCTG 
GTTGATAATA TAAAGACTGT GCTCACTGGT CATAACCGGC AACTTGGAGA TGTCAAACAT 
CTCCTGAAAG CAATGGCTAA GGATGAAGAA GCTGCTCTGG CAGATGGTGA AGATGTTCCC 
TATGAGAACA GTGTTAGGCA ATTCTTAGGT GAATATAAAT CCTGGCAAGA CAACATTCAG 
ACGGTGCTCT TTACGTTAGT CCAAGCTATG GGTCAGGTTC GAAGTCAAGA ACACATTGAA 
ATGCTCCAGG AAATAACTCC CACCTTGAAA GAACTGAAAA CACAAAGTCA GAGTATCTAT 
AATAATTTAG TGGGTTTTGC ATCACCCTTA GTCACCGATG CAACAAATGA ATGTTCGAGT 
CCAACGTCAT CTGCTACTTA TCAGCCATCC TTTGCTGCAG CAGTCCGGAG CAACACTGGC 
CAGAAGACTC AGCCTGATGT CATGTCACAG AATGCTAGAA AGCTGATTCA GAAAAATCTT 
GCCACATCAG CAGATACTCC ACCAAGCACC ATTCCAGGAA CTGGCAAGAG TGTTGCTTGT 
AGTCCTAAAA AGGCTGTCAG AGACCCTAAA ACAGGGAAAG CTGTGCAAGA GAGAAACTCC 
TATGCAGTGA GTGTGTGGAA GAGGGTGAAA GCCAAGCTAG AGGGCCGCGA TGTTGATCCT 
AATAGGAGAA TGTCAGTTGC TGAACAGGTT GACTATGTCA TTAAGGAAGC AACTAATCTA 
GATAACTTGG CTCAGCTGTA TGAAGGTTGG ACAGCCTGGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_011226515.2 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_011224817.1 Ailuropoda melanoleuca SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

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CloneID	OAc28415
Clone ID Related Accession (Same CDS sequence)	XM_011226515.2
Accession Version	XM_011226515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482768000000
Organism	Ailuropoda melanoleuca(giant panda)
Product	serine/threonine-protein kinase SMG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217710.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_011226515.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAT CACAGGAGCG ATCGATGTCT TATTCTGATG AGTCTCGACT GTCGAATCTT 
CTTCGGAGGA TCACCCGGGA AGACGACAGA GACCGAAGAC TGGCTACTGT AAAGCAGTTG 
AAAGAATTTA TTCAGCAACC AGAAAATAAG CTGGTACTAG TTAAACAATT GGATAATATC 
TTGGCTGCTG TACATGATGT GCTTAATGAA AGTAGCAAAT TGCTTCAGGA GTTGAGACAG 
GAGGGAGCTT GCTGTCTTGG CCTTCTTTGT GCTTCTCTGA GCTATGAGGC TGAGAAGATC 
TTCAAATGGA TTTTTAGCAA ATTTAGCTCA TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC 
TTATGTGCCA CCTACAAAGC ATTAGAGACT GTAGGAGAAA AGAAAGCCTT TTCATCGGTA 
ATGCAGCTTG TGATGACCAG CCTACAGTCC ATTCTTGAAA ATGTGGATAC ACCAGAATTA 
CTTTGCAAAT GTGTTAAGTG CATTCTATTG GTGGCTCGAT GTTACCCTCA TATTTTCAGC 
ACTAATTTTA GGGATACAGT TGATATATTA GTTGGATGGC ATATAGATCA TACTCAGAAA 
CCTTCGCTCA CACAGCAGGT GTCTGGGTGG TTGCAGAGTT TGGAGCCATT TTGGGTAGCT 
GATCTTGCAT TTTCTACCAC TCTTCTTGGT CAGTTTCTAG AGGACATGGA GGCATATGCT 
GAGGACCTCA GCCATGTGGC CTCTGGGGAG TCAGTGGATG AAGATGTTCC TCCTCCATCA 
GTGTCGCTAC CAAAGCTGGC CGCGCTCCTT CGAGTGTTCA GTACTGTAGT GAGGAGCATC 
GGGGAGCGCT TCAGCCCAAT TCGGGGTCCT CCAATTACTG AGGCATATGT AACAGATGTT 
CTATACAGAG TAATGAGATG TGTGACGGCT GCAAACCAAG TGTTTTTTTC TGAGGCTGTG 
TTGACAGCTG CTAATGAGTG TGTTGGTGTT TTGCTCGGCA GCTTGGATCC TAGCATGACT 
ATACATTGTG ACATGGTCAT TACATATGGA TTAGATCAAC TGGAGAACTG CCAGACTTGC 
GGTACAGATT ATATCATCTC AGTCTTGAAT TTACTCACAT TGATTGTTGA ACAGATAAAT 
ACAAAACTGC CATCATCATT TGTAGAAAAA CTGTTCATAC CATCATCTAA ACTATTATTT 
TTGCGTTACC ATAAAGAGAA GGAGGTTGTT GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG 
CTCAGCCTGA AGAATATTCC TGTTTTGGAG ACTGCCTATA AGTTAATTTT GGGGGAAATG 
ACTTGTGCCC TAAACAACCT GCTACATAGT CTACAACTTC CTGATGCCTG CTCTGAAATA 
AAACATGAGG CTTTTAAAAA TCACGTATTC AATGTAGACA ATGCAAAATT TGTAGTTATA 
TTTGACCTCA GTGCCCTGAC TACAATTGGA AATGCCAAAA ACTCACTAAT TGGGATGTGG 
GCGCTATCTC CAACTGTCTT TGCACTGCTG AGTAAGAATC TGATGATTGT GCATGGTGAC 
CTGGCTGTTC ACTTCCCTGC CATTCAGTAC GCCGTGCTCT ACACGTTGTA TTCTCATTGT 
ACCAGGCACG ATCACTTTAT ATCTAGTAGT CTTAGTTCTT CGTCCCCATC TTTGTTTGAT 
GGAGCTGTGA TTAGTACTGT AACTACAGCT ACAAAAAAAC ATTTCTCAAT TATACTGAAT 
CTCCTGGGAA TACTGCTTAA GAAAGATAAT CTTAACCAGG ATACAAGGAA ACTGTTAATG 
ACCTGGGCTT TGGAAGTGGC TGTTTTAATG AAGAAGTCTG AAACATATGC ACCTTTATTC 
TCTCTTCCAT CTTTCCATAA ATTTTGCAAA GGCCTTTTAG CCAACACTCT TGTTGAAGAT 
GTGAATATCT GTCTGCAAGC ATGCAGCAGT CTACATGCTT TGTCTTCTTC GTTGCCAGAT 
GATCTTTTAC AGAGGTGTGT CGATGTTTGC CGTGTTCAAC TTGTCCATAG TGGAACTCGT 
ATTCGACAAG CATTTGGAAA GCTGTTGAAG TCAATTCCTT TAGATGTTGT TTTGAGCAAT 
AACAATCACA CAGAAATTCA AGAGATTTCT TTAGCATTAA GAAGTCACAT GAGCAAAGCA 
CCAAGTAACA CATTCCACCC ACAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGG 
AATTCTCACA GAACAGGGAA GGACAATTGG TTAGAAAGAT TGTTCTATAG CTGCCAGAGA 
CTGGATAAGC GTGACCAATC AACAATTCCC CGGAATCTTC TAAAGACAGA TGCTGTCCTT 
TGGCAGTGGG CTATTTGGGA GGCAGCACAG TTCACTGTCC TCTCTAAGTT GAGAACACCA 
TTGGGCAGAG CTCAGGATAC CTTCCAGACC ATTGAAGGTA TCATTAGAAG TCTTGCAGCT 
CACACGTTAA ACCCCGATCA AGATGTTAGT CAGTGGACAA CTGCAGACAA TGATGAAGGC 
CACAGTAGCA ACCAGCTTAG ACTTGTTCTT CTTCTGCAAT ATCTGGAAAA TCTGGAGAAG 
TTAATGTATA ATGCATATGA AGGGTGTGCT AATGCATTAA CATCACCTCC CAAGGTCATT 
AGGACTTTTT TTTATACCAA TCGCCAAACT TGCCAAGACT GGCTGACGCG GATTCGACTC 
TCCATTATGA GGGTTGGGTT GTTGGCAGGC CAGCCCGCTG TGACAGTGAG ACATGGCTTT 
GACTTGCTTA CAGAAATGAA GACAAATCTA TCTCAGGGGA ATGAATTGGA AGTAACCATC 
ATGATGGTGG TAGAAGCACT GTGTGAGCTT CATTGTCCTG AAGCTATACA GGGAATTGCT 
GTCTGGTCGT CTTCCATCGT TGGAAAGAAT CTTCTCTGGA TTAATTCTGT GGCCCAACAG 
GCTGAAGGGA GGTTTGAAAA GGCCTCTGTG GAGTACCAGG AGCACTTGTG CGCCATGACA 
GGGGTCGATT GCTGCATCTC CAGCTTTGAC AAATCTGTTC TCACATTGGC CAACAGTGGG 
CGTAACAGTG CCAGCCCCAA ACATTCTCTG AACGGTGAAT CCAGAAAAAC TGTGCTGTCC 
AAGCCAACTG ACTCTTCTCC TGAGGTTATA AATTATTTGG GAAATAAAGC ATGTGAGTGC 
TACATCTCCA TTGCCGACTG GGCTGCGGTA CAGGAGTGGC AGAATGCCAT CCACGACTTG 
AAGAAGAGTA CCAGCAGTAC TTCTCTCAAC CTGAAAGCTG ATTTCAACTA CATTAAATCA 
CTAAGCAGTT TTGAATCTGG AGAATTTGTT GAATGTACTG AACAATTAGA ATTGTTACCA 
GGAGAAAATA TCAATCTACT TGCTGGAGGA TCAAAAGAGA AAATAGATAT GAAAAAACTG 
CTTCCTAACA TGTTAAGTCC GGATCCAAGG GAACTTCAGA AATCCATTGA AGTTCAGTTG 
TTGAGAAGTT CTGTTTGTTT GGCAACTGCG TTAAACCATA TAGAACAAGA TCAGAAGTGG 
CAGTCCATAA CTGAAAATGT GGTAAAGTAC TTGAAGCAAA CCTCCCGCAT AGCTATTGGA 
CCACTGAGAC TTTCTACTTT AACAGTTTCA CAGTCTCTGC CAGTTCTAAG TACCTTACAG 
CTGTATTGCT CTTCTGCTTT GGAGAACACA GTTTCTAACA GACTTTCAAC AGAGGATTGT 
CTTATTCCAC TCTTCAGTGA AGCTCTGCGT TCATGTAAAC AGCATGATGT GAGGCCATGG 
ATGCAGGCAT TAAGATATAC CATGTACCAG AATCAGTTGT TGGAGAAGAT AAAAGAACAA 
ACAGTTCCAA TTAGAAGCCA TCTCATGGAA TTAGGACTAA CAGCAGCAAA ATTTGCTAGA 
AAACGAGGGA ATGTGTCACT TGCAACAAGA CTGCTGGCAC AGTGTAGTGA GGTTCAGCTG 
GGAAAGACTA CTACTGCACA GGATTTAGTC CAGCATTTTA AAAAACTATC AACTCAAGGT 
CAAGTGGATG AAAAATGGGG GCCTGAACTT GACATTGAAA AAACCAAATT GCTATATACA 
GCAGGCCAGT CAACACATGC AATGGAAATG TTGAGTTCTT GTGCCATATC TTTCTGCAAG 
TCTGCCAAAG CCGAATATGC AGTTGCCAAG TCAATTCTGA CACTGGCTAA ATGGATCCAG 
GCCGAATGGA AAGAAATTTC AGGGCAGCTG AAACAGGTTT ACAGAGCTCA GCAACAGCAG 
AACTTCACAG GTCTTTCTAC TCTGTCTAAA AACATACTTG CTTTAATGGA ACTGCCATCT 
GTTAATGCAA TGGAAGAAGA GTATCCTCGG ATTGAGAGCG AATCTACAGT ACATATTGGA 
GTTGGAGAAC CTGACTTCAT TTTGGGACAG TTGTATCACC TGTCTTCAGT ACAGGCACCT 
GAAGTAGCCA AATCCTGGGC AGCATTGGCT AGTTGGGCTT ATAGGTGGGG CAGAAAGGTG 
GTTGATAATG CCAGTCAGGG AGAAGGTGTT CGTCTGCTGC CTAGAGAAAA ATCTGAAGTT 
CAGAATCTGC TTCCAGACAC TATAACTGAA GAAGAAAAAG AGAGAATATA TGGTATTCTT 
GGACAGGCTG TGTGTCGGCC AGCAGGGATT CAGGATGAGG ACATAACGCT TCAGATAACA 
GAAAGTGAAG ATAATGAGGA AGATGACATG GTTGATGTTA TCTGGCGTCA GTTAATCTCA 
AGCTGCCCGT GGCTTTCAGA ACTTGATGAA GGTGCAACAG AAGGAGTTAT TAAAGTGTGG 
AGAAAGGTTG TAGATAGAAT CTTCAGCCTG TACAAGCTGT CTTGCAGTGC ATATTTTACT 
TTCCTTAAAC TCAATGCCGG TCAGATTCCT TTAGATGAAG ATGACCCTAG GCTACATTTA 
AGTCACAGAG CAGAGCAGAG TACTGATGAT GTGATTGTGA TGGCAACACT GCGCTTGCTC 
AGATTGCTCG TCAAACATGC CGGCGAGCTT AGGCAGTATC TGGAACATGG CTTGGAAACA 
ACACCTACTG CTCCATGGAG AGGAATTATC CCACAGCTTT TCTCACGCTT AAACCATCCT 
GAAGTATATG TGCGCCAAAG TATTTGTAAC CTTCTCTGCC GAGTGGCTCA AGATTCCCCG 
CATCTCATAT TGTACCCTGC GATTGTGGGC ACTATATCAC TTAGTACTGA ATCGCAGGCT 
TCAGGAAATA AGTTTTCGTC TGCAATTCCA ACTTTACTTG GCAACATTCA AGGAGAAGAG 
TTGCTGGTTT CTGAATGTGA GGGAGGAAGC CCTCCTGCTT CTCAGGATAG CAATAAGGAT 
GAACCTAAAA GTGGATTAAA TGAAGACCAA GCCATGATGC AGGATTGCTA CAGCAAAATT 
GTAGATAAGC TGTCCTCTGC AAACCCAACT ATGGTGTTAC AGGTCCAGAT GCTTGTGGCT 
GAGCTGCGCA GGGTCACTGT TCTCTGGGAT GAGCTCTGGT TGGGAGTTCT GTTACAGCAA 
CACATGTATG TCCTGAGACG GATCCAGCAG CTGGAAGATG AGGTGAAGAG AGTCCAGAAC 
AACAACACCT TGCGGAAGGA AGAGAAAATT GCAATCATGC GTGAAAAGCA CACGGCTTTG 
ATGAAGCCCA TTGTGTTTGC TTTGGAGCAC GTAAGGAGCA TCACTGCAGC CCCTGCAGAA 
ACACCTCATG AAAAGTGGTT TCAGGATAAC TATGGTGAAG CAATTGAAAA TGCTCTTGAA 
AAGCTGAAGA CACCATCAAA TCCTGCAAAG CCTGGAAGCA GCTGGATTCC ATTTAAAGAG 
ATAATGCTGA GTTTGCAACA GAGAGCACAG AAACGTGCAA GTTATATCTT GCGTCTTGAA 
GAAATCAGCC CATGGCTGGC TGCCATGACT AACACTGAAA TTGCTCTTCC TGGAGAAGTG 
TCAGCTAGAG ACACTGTCAC AATCCACAGT GTGGGTGGAA CTATCACCAT CTTACCTACC 
AAAACCAAGC CAAAGAAACT TCTCTTTCTA GGGTCAGATG GCAAGAGCTA TCCTTATCTT 
TTCAAAGGAT TAGAAGATTT ACATCTGGAT GAGAGAATAA TGCAATTCCT GTCTATTGTG 
AATACCATGT TTGCTACAAT CAATCGCCAG GAAACACCCC GTTTCCACGC CCGACACTAT 
TCCGTAACGC CACTAGGAAC ACGATCAGGA CTAATCCAGT GGGTGGATGG AGCCACACCC 
TTATTTGGTC TTTACAAACG ATGGCAACAA CGGGAAGCTG CCTTACAGGC ACAGAAGGCC 
CAAGATTCCT ACCAAACTCC TCAGAATCCT GGAATTGTAC CTCGTCCTAG TGAACTTTAT 
TATAGTAAAA TTGGCCCTGC TTTAAAAGCA GTTGGACTTA GTCTCGATGT GTCCCGACGG 
GATTGGCCTC TTCATGTAAT GAAGGCGGTA TTAGAAGAGT TAATGGAGGC CACCCCCCCA 
AATCTCCTTG CTAAAGAGCT TTGGTCCTCT TGCACCACAC CTGATGAATG GTGGAGAGTC 
ACACAGTCTT ATGCCAGATC TACTGCAGTC ATGTCTATGG TTGGATACAT AATTGGCCTT 
GGAGATAGAC ATCTGGATAA TGTTCTCATA GATATGACGA CTGGAGAGGT GGTTCACATA 
GATTACAATG TTTGCTTTGA AAAAGGTAAA AGCCTTAGAG TTCCTGAGAA AGTACCTTTT 
CGAATGACAC AGAATATTGA AACAGCACTG GGTGTAACTG GAGTAGAAGG TGTTTTTAGG 
CTTTCATGTG AGCAGGTTCT ACACATCATG CGGCGTGGCA GAGAGACTCT GCTGACATTG 
CTGGAGGCCT TTGTGTATGA CCCCCTGGTG GACTGGACGG CCGGGGGCGA GGCGGGATTT 
GCCGGCGCTG TCTATGGTGG AGGTGGCCAG CAGGCTGAGA GCAAGCAGAG CAAGAGAGAG 
ATGGAGCGAG AGATCACCCG TAGCCTCTTT TCTTCCAGAG TAGCTGAAAT TAAGGTGAAC 
TGGTTTAAGA ATAGGGATGA GATGCAGGTC GTGCTTCCCA AGCTGGACAG CAGCTTAGAT 
GAATACCTAA GCTTGCAAGA GCAGCTGACA GATGTGGAAA AACTTCAAGG CAAACTGCTG 
GAAGAAATAG AGTTTTTAGA AGGAGCTGAA GGGGTGGACC ATCCTTCTCA CACTCTGCAA 
CACAGGTATT CTGAACACAC TCAACTACAG ACTCAGCAAA GGGCTGTTCA GGAAGCAATC 
CAGGTGAAGC TGAATGAATT TGAACAGTGG ATAACACATT ATCAGGCTGC GTTCAATAAT 
TTAGAAGCAA CACAGCTTGC AAGTCTGCTT CAGGAGATAA GCTCACAAAT GGACCTTGGT 
CCTCCAAGCT ATGTGCCAGC GACAGCCTTT CTACAGAATG CTGGCCAGGC GCACTTGATT 
AGCCAGTGTG AGCAGCTGGA GGGGGAAGTG GGCGCTCTTC TCCAGCAGAG GCGTTCGGTG 
CTCCGAGGTT GCCTGGAACA GCTGCATCAC TATGCAACCG TAGCTCTGCA GTATCCAAAG 
GCCATATTTC AGAAACATCG AATTGAGCAG TGGAAGACCT GGATGGAAGA ACTCATCTGT 
AACACCACAG TAGAGCGCTG TCAAGAACTC TACAGGAAAT ATGAGATGCA GTATGCTCCC 
CAGCCTCCAC CAGCAGTGTG TCAGTTCATC ACTGCCACGG AGATGACCCT GCAGCGGTAC 
GCAGCTGACA TCAACAGCAG GCTGATCAGA CAAGTGGAAC GCTTGAAACA GGAAGCTGTC 
ACTGTGCCTG TTTGTGAAGA TCAGTTGAAA GAAATTGAAC GTTGCATTAA AGTTTTCCTT 
CACGAGAATG GAGAAGAAGG ATCTTTGAGT CTAGCAAGTG TTATTATTTC TGCCCTTTGT 
ACTCTTACAA GGCGGAACCT GATGATGGAA GGTGCAGCTT CAAGTGCTGG GGAGCAGCTG 
GTGGACCTGA CCTCTCGGGA TGGAGCCTGG TTCTTGGAAG AGCTCTGCAG TATGAGCGGG 
AACGTCACTT GCCTGGTGCA GTTGCTGAAG CAGTGCCACC TGGTGCCACA GGACTTGGAC 
ATTCCGAACC CAATGGAAGC TTCTGAGGCA GTTCATTTAG CTAATGGAGT GTATACCTCA 
CTTCAGGAAT TAAATTCAAA TTTCCGGCAA ATCATATTTC CAGAAGCACT GAGATGTTTA 
ATGAAAGGAG AATACACATT AGAAAGTATG CTTCATGAGC TGGACAGTCT TATTGAGCAG 
ACAACTGACG GCGTTCCCCT GCAGACTCTA GTAGAATCTC TTCAGGCCTA TTTAAGAAAT 
GCGGCTATGG GACTCGAGGA GGAAACGCAT GCTCATTACA TCGATGTTGC CAGAATACTT 
CATGCTCAAT ATGGTGAATT AATCCAACCA AGAAACGGTT CGGTTGATGA AACACCAAAA 
ATGTCAGCTG GCCAGATGCT TTTGGTAGCA TTTGATGGCA TGTTTGCCCA AGTTGAAACT 
GCTTTTGGCT TATTAGTTGA AAAGTTAAAT AAGATGGAAA TTCCCGTAGC TTGGCGAAAG 
ATTGACATTA TAAGGGAAGC CAGGAGTACC CAAGTCAATT TTTTTGATGA CGATAATCAC 
CGGCAGGTGC TAGAGGAGAT TTTCTTTCTA AAAAGACTAC AGACAATTAA GGAGTTCTTC 
AGGCTCTGTG GTACCTTTTC TAAAACTTTA TCAGGATCGA GTTCACTTGA AGATCAGAAT 
ACTGTAAATG GACCTGTACA GATTGTCAAC GTGAAAACCC TTTTTAGAAA CTCCTGTTTC 
AGTGAAGACC AGATGGCCAA ACCCATAAAG GCTTTCACAG CTGACTTCGT GAGGCAGCTC 
TTGATTGGAC TCCCGAACCA AGCCCTAGGA CTCACCCTCT GCAGTTTTAT CAGTGCTCTA 
GGGGTAGACA TTATTGCTCA AGTAGAGGCA AAGGATTTTG GTGCTGAAAG CAAAGTTTCT 
GTTGATGATC TTTGTAAGAA AGCAGTGGAG CACAACATTC AGATTGGGAA GTTCTCTCAG 
CTGGTCATGA ACAGGGCAAC TGTGTTAGCA AGTTCCTATG ATACTGCCTG GAAGAAGCAT 
GACTTGGTGC GCAGGCTGGA AACCAGTATT TCTTCTTGTA AGACAAGCCT GCAGCGTGTT 
CAGCTGCATA TTGCCATGTT TCAATGGCAG CATGAAGACC TCCTTATCAA TAGACCACAA 
GCCATGTCAG TCACACCTCC CCCTCGGTCC GCCATCCTAA CCAGCATGAA AAAGAAACTC 
CATACTCTGA GCCAAATTGA AACTTCAATT GCAACAGTTC AGGAGAAACT AGCAGCCCTT 
GAAGCAAGTA TTGAACAGCG ACTCAAGTGG GCAGGTGGTG CCAACCCTGC ACTGGCACCT 
GTGTTGCAGG ATTTTGAAGC AACAATAGCT GAAAGAAGAA ATCTTGTCCT TAAAGAGAGC 
CAAAGAGCAA GCCAGGTCAC TTTCCTCTGC AGCAATATCA TTCATTTTGA AAGTTTACGA 
ACTAGGACTG CAGAAGCCTT AAACCTGGAT GCTGCATTAT TTGAACTAAT CAAACGATGT 
CAGCAAATGT GTTCATTTGC ATCACAGTTT AACAGTTCAG TCTCTGAGTT AGAGCTTCGT 
CTGTTACAGA GAGTGGACAC CAGTCTTGAA CATCCTATTG GCAGCTCTGA ATGGCTTTTG 
TCAGCACACA AACAATTGAC CCAGGATATG TCTACTCAGA GAGCAATTCA GACAGAGAAG 
GAGCAACAGA TAGAAACAGT CTGTGAAACA ATTCAGAATC TGGTTGATAA TATAAAGACT 
GTGCTCACTG GTCATAACCG GCAACTTGGA GATGTCAAAC ATCTCCTGAA AGCAATGGCT 
AAGGATGAAG AAGCTGCTCT GGCAGATGGT GAAGATGTTC CCTATGAGAA CAGTGTTAGG 
CAATTCTTAG GTGAATATAA ATCCTGGCAA GACAACATTC AGACGGTGCT CTTTACGTTA 
GTCCAAGCTA TGGGTCAGGT TCGAAGTCAA GAACACATTG AAATGCTCCA GGAAATAACT 
CCCACCTTGA AAGAACTGAA AACACAAAGT CAGAGTATCT ATAATAATTT AGTGGGTTTT 
GCATCACCCT TAGTCACCGA TGCAACAAAT GAATGTTCGA GTCCAACGTC ATCTGCTACT 
TATCAGCCAT CCTTTGCTGC AGCAGTCCGG AGCAACACTG GCCAGAAGAC TCAGCCTGAT 
GTCATGTCAC AGAATGCTAG AAAGCTGATT CAGAAAAATC TTGCCACATC AGCAGATACT 
CCACCAAGCA CCATTCCAGG AACTGGCAAG AGTGTTGCTT GTAGTCCTAA AAAGGCTGTC 
AGAGACCCTA AAACAGGGAA AGCTGTGCAA GAGAGAAACT CCTATGCAGT GAGTGTGTGG 
AAGAGGGTGA AAGCCAAGCT AGAGGGCCGC GATGTTGATC CTAATAGGAG AATGTCAGTT 
GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG 
TATGAAGGTT GGACAGCCTG GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011224817.1
CDS	337..10923
Translation	MRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPDACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHGDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEVAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHSSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANSGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGEFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNHIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSAKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQQQQNFTGLSTLSKNILALMELPSVNAMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDEGATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRAEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSTESQASGNKFSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGEAIENALEKLKTPSNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKAVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMQVVLPKLDSSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISSQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPAVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASEAVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDSLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARILHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFGLLVEKLNKMEIPVAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALEASIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTSLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHIEMLQEITPTLKELKTQSQSIYNNLVGFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTIPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_011226515.2
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011226515.2

ORF Insert Sequence:

ATGAGGAAAT CACAGGAGCG ATCGATGTCT TATTCTGATG AGTCTCGACT GTCGAATCTT 
CTTCGGAGGA TCACCCGGGA AGACGACAGA GACCGAAGAC TGGCTACTGT AAAGCAGTTG 
AAAGAATTTA TTCAGCAACC AGAAAATAAG CTGGTACTAG TTAAACAATT GGATAATATC 
TTGGCTGCTG TACATGATGT GCTTAATGAA AGTAGCAAAT TGCTTCAGGA GTTGAGACAG 
GAGGGAGCTT GCTGTCTTGG CCTTCTTTGT GCTTCTCTGA GCTATGAGGC TGAGAAGATC 
TTCAAATGGA TTTTTAGCAA ATTTAGCTCA TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC 
TTATGTGCCA CCTACAAAGC ATTAGAGACT GTAGGAGAAA AGAAAGCCTT TTCATCGGTA 
ATGCAGCTTG TGATGACCAG CCTACAGTCC ATTCTTGAAA ATGTGGATAC ACCAGAATTA 
CTTTGCAAAT GTGTTAAGTG CATTCTATTG GTGGCTCGAT GTTACCCTCA TATTTTCAGC 
ACTAATTTTA GGGATACAGT TGATATATTA GTTGGATGGC ATATAGATCA TACTCAGAAA 
CCTTCGCTCA CACAGCAGGT GTCTGGGTGG TTGCAGAGTT TGGAGCCATT TTGGGTAGCT 
GATCTTGCAT TTTCTACCAC TCTTCTTGGT CAGTTTCTAG AGGACATGGA GGCATATGCT 
GAGGACCTCA GCCATGTGGC CTCTGGGGAG TCAGTGGATG AAGATGTTCC TCCTCCATCA 
GTGTCGCTAC CAAAGCTGGC CGCGCTCCTT CGAGTGTTCA GTACTGTAGT GAGGAGCATC 
GGGGAGCGCT TCAGCCCAAT TCGGGGTCCT CCAATTACTG AGGCATATGT AACAGATGTT 
CTATACAGAG TAATGAGATG TGTGACGGCT GCAAACCAAG TGTTTTTTTC TGAGGCTGTG 
TTGACAGCTG CTAATGAGTG TGTTGGTGTT TTGCTCGGCA GCTTGGATCC TAGCATGACT 
ATACATTGTG ACATGGTCAT TACATATGGA TTAGATCAAC TGGAGAACTG CCAGACTTGC 
GGTACAGATT ATATCATCTC AGTCTTGAAT TTACTCACAT TGATTGTTGA ACAGATAAAT 
ACAAAACTGC CATCATCATT TGTAGAAAAA CTGTTCATAC CATCATCTAA ACTATTATTT 
TTGCGTTACC ATAAAGAGAA GGAGGTTGTT GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG 
CTCAGCCTGA AGAATATTCC TGTTTTGGAG ACTGCCTATA AGTTAATTTT GGGGGAAATG 
ACTTGTGCCC TAAACAACCT GCTACATAGT CTACAACTTC CTGATGCCTG CTCTGAAATA 
AAACATGAGG CTTTTAAAAA TCACGTATTC AATGTAGACA ATGCAAAATT TGTAGTTATA 
TTTGACCTCA GTGCCCTGAC TACAATTGGA AATGCCAAAA ACTCACTAAT TGGGATGTGG 
GCGCTATCTC CAACTGTCTT TGCACTGCTG AGTAAGAATC TGATGATTGT GCATGGTGAC 
CTGGCTGTTC ACTTCCCTGC CATTCAGTAC GCCGTGCTCT ACACGTTGTA TTCTCATTGT 
ACCAGGCACG ATCACTTTAT ATCTAGTAGT CTTAGTTCTT CGTCCCCATC TTTGTTTGAT 
GGAGCTGTGA TTAGTACTGT AACTACAGCT ACAAAAAAAC ATTTCTCAAT TATACTGAAT 
CTCCTGGGAA TACTGCTTAA GAAAGATAAT CTTAACCAGG ATACAAGGAA ACTGTTAATG 
ACCTGGGCTT TGGAAGTGGC TGTTTTAATG AAGAAGTCTG AAACATATGC ACCTTTATTC 
TCTCTTCCAT CTTTCCATAA ATTTTGCAAA GGCCTTTTAG CCAACACTCT TGTTGAAGAT 
GTGAATATCT GTCTGCAAGC ATGCAGCAGT CTACATGCTT TGTCTTCTTC GTTGCCAGAT 
GATCTTTTAC AGAGGTGTGT CGATGTTTGC CGTGTTCAAC TTGTCCATAG TGGAACTCGT 
ATTCGACAAG CATTTGGAAA GCTGTTGAAG TCAATTCCTT TAGATGTTGT TTTGAGCAAT 
AACAATCACA CAGAAATTCA AGAGATTTCT TTAGCATTAA GAAGTCACAT GAGCAAAGCA 
CCAAGTAACA CATTCCACCC ACAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGG 
AATTCTCACA GAACAGGGAA GGACAATTGG TTAGAAAGAT TGTTCTATAG CTGCCAGAGA 
CTGGATAAGC GTGACCAATC AACAATTCCC CGGAATCTTC TAAAGACAGA TGCTGTCCTT 
TGGCAGTGGG CTATTTGGGA GGCAGCACAG TTCACTGTCC TCTCTAAGTT GAGAACACCA 
TTGGGCAGAG CTCAGGATAC CTTCCAGACC ATTGAAGGTA TCATTAGAAG TCTTGCAGCT 
CACACGTTAA ACCCCGATCA AGATGTTAGT CAGTGGACAA CTGCAGACAA TGATGAAGGC 
CACAGTAGCA ACCAGCTTAG ACTTGTTCTT CTTCTGCAAT ATCTGGAAAA TCTGGAGAAG 
TTAATGTATA ATGCATATGA AGGGTGTGCT AATGCATTAA CATCACCTCC CAAGGTCATT 
AGGACTTTTT TTTATACCAA TCGCCAAACT TGCCAAGACT GGCTGACGCG GATTCGACTC 
TCCATTATGA GGGTTGGGTT GTTGGCAGGC CAGCCCGCTG TGACAGTGAG ACATGGCTTT 
GACTTGCTTA CAGAAATGAA GACAAATCTA TCTCAGGGGA ATGAATTGGA AGTAACCATC 
ATGATGGTGG TAGAAGCACT GTGTGAGCTT CATTGTCCTG AAGCTATACA GGGAATTGCT 
GTCTGGTCGT CTTCCATCGT TGGAAAGAAT CTTCTCTGGA TTAATTCTGT GGCCCAACAG 
GCTGAAGGGA GGTTTGAAAA GGCCTCTGTG GAGTACCAGG AGCACTTGTG CGCCATGACA 
GGGGTCGATT GCTGCATCTC CAGCTTTGAC AAATCTGTTC TCACATTGGC CAACAGTGGG 
CGTAACAGTG CCAGCCCCAA ACATTCTCTG AACGGTGAAT CCAGAAAAAC TGTGCTGTCC 
AAGCCAACTG ACTCTTCTCC TGAGGTTATA AATTATTTGG GAAATAAAGC ATGTGAGTGC 
TACATCTCCA TTGCCGACTG GGCTGCGGTA CAGGAGTGGC AGAATGCCAT CCACGACTTG 
AAGAAGAGTA CCAGCAGTAC TTCTCTCAAC CTGAAAGCTG ATTTCAACTA CATTAAATCA 
CTAAGCAGTT TTGAATCTGG AGAATTTGTT GAATGTACTG AACAATTAGA ATTGTTACCA 
GGAGAAAATA TCAATCTACT TGCTGGAGGA TCAAAAGAGA AAATAGATAT GAAAAAACTG 
CTTCCTAACA TGTTAAGTCC GGATCCAAGG GAACTTCAGA AATCCATTGA AGTTCAGTTG 
TTGAGAAGTT CTGTTTGTTT GGCAACTGCG TTAAACCATA TAGAACAAGA TCAGAAGTGG 
CAGTCCATAA CTGAAAATGT GGTAAAGTAC TTGAAGCAAA CCTCCCGCAT AGCTATTGGA 
CCACTGAGAC TTTCTACTTT AACAGTTTCA CAGTCTCTGC CAGTTCTAAG TACCTTACAG 
CTGTATTGCT CTTCTGCTTT GGAGAACACA GTTTCTAACA GACTTTCAAC AGAGGATTGT 
CTTATTCCAC TCTTCAGTGA AGCTCTGCGT TCATGTAAAC AGCATGATGT GAGGCCATGG 
ATGCAGGCAT TAAGATATAC CATGTACCAG AATCAGTTGT TGGAGAAGAT AAAAGAACAA 
ACAGTTCCAA TTAGAAGCCA TCTCATGGAA TTAGGACTAA CAGCAGCAAA ATTTGCTAGA 
AAACGAGGGA ATGTGTCACT TGCAACAAGA CTGCTGGCAC AGTGTAGTGA GGTTCAGCTG 
GGAAAGACTA CTACTGCACA GGATTTAGTC CAGCATTTTA AAAAACTATC AACTCAAGGT 
CAAGTGGATG AAAAATGGGG GCCTGAACTT GACATTGAAA AAACCAAATT GCTATATACA 
GCAGGCCAGT CAACACATGC AATGGAAATG TTGAGTTCTT GTGCCATATC TTTCTGCAAG 
TCTGCCAAAG CCGAATATGC AGTTGCCAAG TCAATTCTGA CACTGGCTAA ATGGATCCAG 
GCCGAATGGA AAGAAATTTC AGGGCAGCTG AAACAGGTTT ACAGAGCTCA GCAACAGCAG 
AACTTCACAG GTCTTTCTAC TCTGTCTAAA AACATACTTG CTTTAATGGA ACTGCCATCT 
GTTAATGCAA TGGAAGAAGA GTATCCTCGG ATTGAGAGCG AATCTACAGT ACATATTGGA 
GTTGGAGAAC CTGACTTCAT TTTGGGACAG TTGTATCACC TGTCTTCAGT ACAGGCACCT 
GAAGTAGCCA AATCCTGGGC AGCATTGGCT AGTTGGGCTT ATAGGTGGGG CAGAAAGGTG 
GTTGATAATG CCAGTCAGGG AGAAGGTGTT CGTCTGCTGC CTAGAGAAAA ATCTGAAGTT 
CAGAATCTGC TTCCAGACAC TATAACTGAA GAAGAAAAAG AGAGAATATA TGGTATTCTT 
GGACAGGCTG TGTGTCGGCC AGCAGGGATT CAGGATGAGG ACATAACGCT TCAGATAACA 
GAAAGTGAAG ATAATGAGGA AGATGACATG GTTGATGTTA TCTGGCGTCA GTTAATCTCA 
AGCTGCCCGT GGCTTTCAGA ACTTGATGAA GGTGCAACAG AAGGAGTTAT TAAAGTGTGG 
AGAAAGGTTG TAGATAGAAT CTTCAGCCTG TACAAGCTGT CTTGCAGTGC ATATTTTACT 
TTCCTTAAAC TCAATGCCGG TCAGATTCCT TTAGATGAAG ATGACCCTAG GCTACATTTA 
AGTCACAGAG CAGAGCAGAG TACTGATGAT GTGATTGTGA TGGCAACACT GCGCTTGCTC 
AGATTGCTCG TCAAACATGC CGGCGAGCTT AGGCAGTATC TGGAACATGG CTTGGAAACA 
ACACCTACTG CTCCATGGAG AGGAATTATC CCACAGCTTT TCTCACGCTT AAACCATCCT 
GAAGTATATG TGCGCCAAAG TATTTGTAAC CTTCTCTGCC GAGTGGCTCA AGATTCCCCG 
CATCTCATAT TGTACCCTGC GATTGTGGGC ACTATATCAC TTAGTACTGA ATCGCAGGCT 
TCAGGAAATA AGTTTTCGTC TGCAATTCCA ACTTTACTTG GCAACATTCA AGGAGAAGAG 
TTGCTGGTTT CTGAATGTGA GGGAGGAAGC CCTCCTGCTT CTCAGGATAG CAATAAGGAT 
GAACCTAAAA GTGGATTAAA TGAAGACCAA GCCATGATGC AGGATTGCTA CAGCAAAATT 
GTAGATAAGC TGTCCTCTGC AAACCCAACT ATGGTGTTAC AGGTCCAGAT GCTTGTGGCT 
GAGCTGCGCA GGGTCACTGT TCTCTGGGAT GAGCTCTGGT TGGGAGTTCT GTTACAGCAA 
CACATGTATG TCCTGAGACG GATCCAGCAG CTGGAAGATG AGGTGAAGAG AGTCCAGAAC 
AACAACACCT TGCGGAAGGA AGAGAAAATT GCAATCATGC GTGAAAAGCA CACGGCTTTG 
ATGAAGCCCA TTGTGTTTGC TTTGGAGCAC GTAAGGAGCA TCACTGCAGC CCCTGCAGAA 
ACACCTCATG AAAAGTGGTT TCAGGATAAC TATGGTGAAG CAATTGAAAA TGCTCTTGAA 
AAGCTGAAGA CACCATCAAA TCCTGCAAAG CCTGGAAGCA GCTGGATTCC ATTTAAAGAG 
ATAATGCTGA GTTTGCAACA GAGAGCACAG AAACGTGCAA GTTATATCTT GCGTCTTGAA 
GAAATCAGCC CATGGCTGGC TGCCATGACT AACACTGAAA TTGCTCTTCC TGGAGAAGTG 
TCAGCTAGAG ACACTGTCAC AATCCACAGT GTGGGTGGAA CTATCACCAT CTTACCTACC 
AAAACCAAGC CAAAGAAACT TCTCTTTCTA GGGTCAGATG GCAAGAGCTA TCCTTATCTT 
TTCAAAGGAT TAGAAGATTT ACATCTGGAT GAGAGAATAA TGCAATTCCT GTCTATTGTG 
AATACCATGT TTGCTACAAT CAATCGCCAG GAAACACCCC GTTTCCACGC CCGACACTAT 
TCCGTAACGC CACTAGGAAC ACGATCAGGA CTAATCCAGT GGGTGGATGG AGCCACACCC 
TTATTTGGTC TTTACAAACG ATGGCAACAA CGGGAAGCTG CCTTACAGGC ACAGAAGGCC 
CAAGATTCCT ACCAAACTCC TCAGAATCCT GGAATTGTAC CTCGTCCTAG TGAACTTTAT 
TATAGTAAAA TTGGCCCTGC TTTAAAAGCA GTTGGACTTA GTCTCGATGT GTCCCGACGG 
GATTGGCCTC TTCATGTAAT GAAGGCGGTA TTAGAAGAGT TAATGGAGGC CACCCCCCCA 
AATCTCCTTG CTAAAGAGCT TTGGTCCTCT TGCACCACAC CTGATGAATG GTGGAGAGTC 
ACACAGTCTT ATGCCAGATC TACTGCAGTC ATGTCTATGG TTGGATACAT AATTGGCCTT 
GGAGATAGAC ATCTGGATAA TGTTCTCATA GATATGACGA CTGGAGAGGT GGTTCACATA 
GATTACAATG TTTGCTTTGA AAAAGGTAAA AGCCTTAGAG TTCCTGAGAA AGTACCTTTT 
CGAATGACAC AGAATATTGA AACAGCACTG GGTGTAACTG GAGTAGAAGG TGTTTTTAGG 
CTTTCATGTG AGCAGGTTCT ACACATCATG CGGCGTGGCA GAGAGACTCT GCTGACATTG 
CTGGAGGCCT TTGTGTATGA CCCCCTGGTG GACTGGACGG CCGGGGGCGA GGCGGGATTT 
GCCGGCGCTG TCTATGGTGG AGGTGGCCAG CAGGCTGAGA GCAAGCAGAG CAAGAGAGAG 
ATGGAGCGAG AGATCACCCG TAGCCTCTTT TCTTCCAGAG TAGCTGAAAT TAAGGTGAAC 
TGGTTTAAGA ATAGGGATGA GATGCAGGTC GTGCTTCCCA AGCTGGACAG CAGCTTAGAT 
GAATACCTAA GCTTGCAAGA GCAGCTGACA GATGTGGAAA AACTTCAAGG CAAACTGCTG 
GAAGAAATAG AGTTTTTAGA AGGAGCTGAA GGGGTGGACC ATCCTTCTCA CACTCTGCAA 
CACAGGTATT CTGAACACAC TCAACTACAG ACTCAGCAAA GGGCTGTTCA GGAAGCAATC 
CAGGTGAAGC TGAATGAATT TGAACAGTGG ATAACACATT ATCAGGCTGC GTTCAATAAT 
TTAGAAGCAA CACAGCTTGC AAGTCTGCTT CAGGAGATAA GCTCACAAAT GGACCTTGGT 
CCTCCAAGCT ATGTGCCAGC GACAGCCTTT CTACAGAATG CTGGCCAGGC GCACTTGATT 
AGCCAGTGTG AGCAGCTGGA GGGGGAAGTG GGCGCTCTTC TCCAGCAGAG GCGTTCGGTG 
CTCCGAGGTT GCCTGGAACA GCTGCATCAC TATGCAACCG TAGCTCTGCA GTATCCAAAG 
GCCATATTTC AGAAACATCG AATTGAGCAG TGGAAGACCT GGATGGAAGA ACTCATCTGT 
AACACCACAG TAGAGCGCTG TCAAGAACTC TACAGGAAAT ATGAGATGCA GTATGCTCCC 
CAGCCTCCAC CAGCAGTGTG TCAGTTCATC ACTGCCACGG AGATGACCCT GCAGCGGTAC 
GCAGCTGACA TCAACAGCAG GCTGATCAGA CAAGTGGAAC GCTTGAAACA GGAAGCTGTC 
ACTGTGCCTG TTTGTGAAGA TCAGTTGAAA GAAATTGAAC GTTGCATTAA AGTTTTCCTT 
CACGAGAATG GAGAAGAAGG ATCTTTGAGT CTAGCAAGTG TTATTATTTC TGCCCTTTGT 
ACTCTTACAA GGCGGAACCT GATGATGGAA GGTGCAGCTT CAAGTGCTGG GGAGCAGCTG 
GTGGACCTGA CCTCTCGGGA TGGAGCCTGG TTCTTGGAAG AGCTCTGCAG TATGAGCGGG 
AACGTCACTT GCCTGGTGCA GTTGCTGAAG CAGTGCCACC TGGTGCCACA GGACTTGGAC 
ATTCCGAACC CAATGGAAGC TTCTGAGGCA GTTCATTTAG CTAATGGAGT GTATACCTCA 
CTTCAGGAAT TAAATTCAAA TTTCCGGCAA ATCATATTTC CAGAAGCACT GAGATGTTTA 
ATGAAAGGAG AATACACATT AGAAAGTATG CTTCATGAGC TGGACAGTCT TATTGAGCAG 
ACAACTGACG GCGTTCCCCT GCAGACTCTA GTAGAATCTC TTCAGGCCTA TTTAAGAAAT 
GCGGCTATGG GACTCGAGGA GGAAACGCAT GCTCATTACA TCGATGTTGC CAGAATACTT 
CATGCTCAAT ATGGTGAATT AATCCAACCA AGAAACGGTT CGGTTGATGA AACACCAAAA 
ATGTCAGCTG GCCAGATGCT TTTGGTAGCA TTTGATGGCA TGTTTGCCCA AGTTGAAACT 
GCTTTTGGCT TATTAGTTGA AAAGTTAAAT AAGATGGAAA TTCCCGTAGC TTGGCGAAAG 
ATTGACATTA TAAGGGAAGC CAGGAGTACC CAAGTCAATT TTTTTGATGA CGATAATCAC 
CGGCAGGTGC TAGAGGAGAT TTTCTTTCTA AAAAGACTAC AGACAATTAA GGAGTTCTTC 
AGGCTCTGTG GTACCTTTTC TAAAACTTTA TCAGGATCGA GTTCACTTGA AGATCAGAAT 
ACTGTAAATG GACCTGTACA GATTGTCAAC GTGAAAACCC TTTTTAGAAA CTCCTGTTTC 
AGTGAAGACC AGATGGCCAA ACCCATAAAG GCTTTCACAG CTGACTTCGT GAGGCAGCTC 
TTGATTGGAC TCCCGAACCA AGCCCTAGGA CTCACCCTCT GCAGTTTTAT CAGTGCTCTA 
GGGGTAGACA TTATTGCTCA AGTAGAGGCA AAGGATTTTG GTGCTGAAAG CAAAGTTTCT 
GTTGATGATC TTTGTAAGAA AGCAGTGGAG CACAACATTC AGATTGGGAA GTTCTCTCAG 
CTGGTCATGA ACAGGGCAAC TGTGTTAGCA AGTTCCTATG ATACTGCCTG GAAGAAGCAT 
GACTTGGTGC GCAGGCTGGA AACCAGTATT TCTTCTTGTA AGACAAGCCT GCAGCGTGTT 
CAGCTGCATA TTGCCATGTT TCAATGGCAG CATGAAGACC TCCTTATCAA TAGACCACAA 
GCCATGTCAG TCACACCTCC CCCTCGGTCC GCCATCCTAA CCAGCATGAA AAAGAAACTC 
CATACTCTGA GCCAAATTGA AACTTCAATT GCAACAGTTC AGGAGAAACT AGCAGCCCTT 
GAAGCAAGTA TTGAACAGCG ACTCAAGTGG GCAGGTGGTG CCAACCCTGC ACTGGCACCT 
GTGTTGCAGG ATTTTGAAGC AACAATAGCT GAAAGAAGAA ATCTTGTCCT TAAAGAGAGC 
CAAAGAGCAA GCCAGGTCAC TTTCCTCTGC AGCAATATCA TTCATTTTGA AAGTTTACGA 
ACTAGGACTG CAGAAGCCTT AAACCTGGAT GCTGCATTAT TTGAACTAAT CAAACGATGT 
CAGCAAATGT GTTCATTTGC ATCACAGTTT AACAGTTCAG TCTCTGAGTT AGAGCTTCGT 
CTGTTACAGA GAGTGGACAC CAGTCTTGAA CATCCTATTG GCAGCTCTGA ATGGCTTTTG 
TCAGCACACA AACAATTGAC CCAGGATATG TCTACTCAGA GAGCAATTCA GACAGAGAAG 
GAGCAACAGA TAGAAACAGT CTGTGAAACA ATTCAGAATC TGGTTGATAA TATAAAGACT 
GTGCTCACTG GTCATAACCG GCAACTTGGA GATGTCAAAC ATCTCCTGAA AGCAATGGCT 
AAGGATGAAG AAGCTGCTCT GGCAGATGGT GAAGATGTTC CCTATGAGAA CAGTGTTAGG 
CAATTCTTAG GTGAATATAA ATCCTGGCAA GACAACATTC AGACGGTGCT CTTTACGTTA 
GTCCAAGCTA TGGGTCAGGT TCGAAGTCAA GAACACATTG AAATGCTCCA GGAAATAACT 
CCCACCTTGA AAGAACTGAA AACACAAAGT CAGAGTATCT ATAATAATTT AGTGGGTTTT 
GCATCACCCT TAGTCACCGA TGCAACAAAT GAATGTTCGA GTCCAACGTC ATCTGCTACT 
TATCAGCCAT CCTTTGCTGC AGCAGTCCGG AGCAACACTG GCCAGAAGAC TCAGCCTGAT 
GTCATGTCAC AGAATGCTAG AAAGCTGATT CAGAAAAATC TTGCCACATC AGCAGATACT 
CCACCAAGCA CCATTCCAGG AACTGGCAAG AGTGTTGCTT GTAGTCCTAA AAAGGCTGTC 
AGAGACCCTA AAACAGGGAA AGCTGTGCAA GAGAGAAACT CCTATGCAGT GAGTGTGTGG 
AAGAGGGTGA AAGCCAAGCT AGAGGGCCGC GATGTTGATC CTAATAGGAG AATGTCAGTT 
GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG 
TATGAAGGTT GGACAGCCTG GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SMG1 cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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