PIEZO1 cDNA ORF clone, Felis catus(Domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIEZO1
Entrez Gene ID	101098421
Full Name	piezo type mechanosensitive ion channel component 1
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023244898.1	XP_023100666.1	LOW QUALITY PROTEIN: piezo-type mechanosensitive ion channel component 1
XM_019818972.1	XP_019674531.1	piezo-type mechanosensitive ion channel component 1

Pathways from BioSystems
Gene Ontology

The following PIEZO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIEZO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFb54552	XM_019818972.1 Latest version!	Felis catus piezo type mechanosensitive ion channel component 1 (PIEZO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb65504	XM_023244898.1 Latest version!	Felis catus piezo type mechanosensitive ion channel component 1 (PIEZO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIEZO1 ( XM_019818972.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019674531.1 Felis catus piezo type mechanosensitive ion channel component 1 (PIEZO1), mRNA.

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CloneID	OFb54552
Clone ID Related Accession (Same CDS sequence)	XM_019818972.1
Accession Version	XM_019818972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	piezo-type mechanosensitive ion channel component 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018737.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGATACCTG AGACTCTGGA GGCTGGTCTC CTGTCCTGGG CGTGGGCAGC TCCCCGGGGA 
CCCTCCTGCT GTCCCTCTGA TGGCAAGACC GGCTGCCATT GGCTCTGCTG CTCTTCTGTG 
TGCCTCCTCG TCCTCTGCCA GGCTTTGGCT GGAAATGCCT GCCTGTGCCG CTTCAACGCC 
CTGTCTCTGG TCTACCTGCT GTTTCTGCTG CTGTTGCCCT GGTTCCCGGG CCCCTCTCCG 
CACAGCGTCC CAGGTCACAC TGGTCGCCTC CTCCGAGCCC TGCTGCTTTT CAGCCTCCTC 
TTCCTGGCAG CCCACCTGGC CTTCCAGATA TGCCTGCACA CCGTGCCCCG CCTGGACCAG 
CTCCTGGGGC CAAGCTGCAG CCCCTGGCAG ATCCTGTCCC AGCACGTAGG GGTGACGAGG 
CTGGACCTGA AGGACATCCC CAATGCCGTC CGGCTGGTGG CCCCTGACCT GGGTGTCCTG 
GTGGTCTCAG CCGTGTGCCT CGGCCTCTGT GGGCGCCTGG CACGGGAAGC CCGGCGGAGC 
CAGCCCGCCC AGGAGCAGGA CGATGAGGAC AGGGAAGCGG ACGCCGGCTC CCCGGCGGGG 
CCTCGCGGAG CCCCCGTGCC GGCCCCCAAG CAGAGGTCTC GGCTGGGCTC GATTTCCATT 
TTGCGCGAAG CAAGAGGCGA AATCGCCGGC CTCCACAGCT GGCTCCCCGG GGACTTGCCC 
TGCGCACAGA GCCGGATGGG AGTCGGGGCC ATTTTGTCCG ACCCGTCCTT TCACAGTCTT 
GGAAACCAAG ATGTGGAGTG GGTGCACCCC AGGCTGTCGG AGCCTAGAGA CGTGTCTCCC 
CTGCACGGTC TGGGCCATCT CATCATGGAC CAGAGCTACG TGTGTGCCCT CATCGCCATG 
ATGGTAAGGA GCGGCTGGCC TGGGCAGGCC CGTCCGGGGG GCCCTTGCTG CTGGTTCCCT 
CGTGATGTTC TGGGGGGGCG GATGGCGGCC TGCAAGGGCC TCATCCCCCG GGAAGGGACG 
GAGGCTGCTG GGTTCGGACG GGAGGGCCCG GAGCAGGGCG ACGGCCTCAG CAGACCCCTC 
GGACACGGCG TGCCCGCGGT GCCGGGTGTT CCTCCGGCCT TCGGGTCACG GGGGCAGCAC 
ATCGGCCGGG CCCCAGGGGA GGCCTTTCTT GGGGCCCACC CTAGGCAGGA CGCGGTCAGC 
GGGACCCCGC TGCTGCAGGA GGAGGAGGAG GTGGCCAGGG AGGAGGGACT GAGCGAGGCC 
GGCCCCCAGC AGGCCGTGCA GGTCCCCGAA GCTGCCGCCA GCAAGTGGGG CCTGGTGGCC 
GAGCGGCTGC TGGACCTGGC TGACCGCTTC TCCGAGGTCC TCACCCGCGT GCAGGTGTTG 
GTGCGACGCC TGCTGGAGCT GCACGTCCTC AAGCTGGTGG CCCTGTACAC TGTGTGGGTG 
GCCCTGAAGG AGGTGTCGGT GATGAACCTG CTTCTGGTCG TGCTGTGGGC CTTCGCCCTG 
CCCTACCCCC GCTTCCGGCC CATGGCCTCC TGCCTGTCCA CCGTGTGGAC CTGTATCATC 
ATCGTGTGCA AGATGCTGTA TCAGCTGAAG GTCGTCAGCC CGCACGAATA CTCCAGCAAC 
TGCACCGAGC CCCTCCTCAA CAGCACCAAC CTGCAGAAAA CGGAGATCAA GCAGTCCCTG 
CTGTACCGGG GACCCGTCGA CCCTGCCAAC TGGTTTGGGG TCCGGAAGGG CTTCCCGAAC 
CTGGGCTACA TCCAGAACCA CCTGCTGGTC CTGCTGCTGC TGGTGTTCGA GGCCATTGTT 
TACCGGCGCC AGGAGCACCA CCGTCGGCAG CACCAGCCGG CCCCGCTGCC TGCCCAGGCC 
GTCTGTGCCG ACGGCACCCG CCAGCGGCTG GACCAAGATC TTCTCAGCTG CTTCAAGTAC 
TTCGTCAACT TCTTTTTCTA CAAATTCGGG CTGGAGATCT GCTTCCTGAT GGCCGTGAAC 
GTGATTGGGC AGCGCATGAA TTTCATGGTC ATCCTGCACG GCTGCTGGCT GGTGGCCATT 
CTTACCCGCC GGCACCGCGG AGCCATCGCC CGCCTCTGGC CCAACTACTG TCTGTTCCTG 
GCGTTGTTCT TCCTGTACCA GTACCTGCTC TGCCTGGGCA TCCCCCCGGC CCTGTGCCTC 
GACTACCCGT GGCGGTGGAG CCAGGCCATC CCCATGAACT CCGCGCTCAT CAAGTGGCTG 
TACCTGCCCG ACTTCTTCAG AACCCCCAAC TCCACCAACC TCATCAGTGA GCGCCTGCGG 
CCGGGCCCCA GAGGCGGGGG CGCGCCTCAC CGGCTCCCCT GGGCCGAGGC TGCCGGGAGG 
AGGTGGGGCC GCCCGGGCCC CGCGGTGACG CCCCCGTCTG GCAGGTCCTA CCTCGACATG 
CTGAAGGTGG CCGTCTTCCG CTACCTCTTC TGGCTGGTGC TGGTGGTGGT GTTCGTCACG 
GGAGCCACCC GCATCAGTAT CTTCGGGCTG GGCTACCTGC TGGCCTGCTT CTACCTGCTG 
CTCTTCGGCA CCAGCCTGTT GCAGAAGCAC ACGCGTGCCC GCCTCCTGCT GTGGGACTGC 
CTCATTCTGT ACAACGTCAC CGTCATCATC TCCAAGAACA TGCTTTCGCT CCTGTCCTGC 
GTGTTCGTGG AGCAAATGCA GAGCAGCTTC TGCTGGGTCA TCCAGCTCTT CAGCCTCGTG 
TGCACCGTCA AAGGCTACTA TGACCCCAAG GAGATGCTGA GCCGGGACCG GGACTGCCTG 
CTGCCCGTGG AGGAGGCCGG CGTGCTCTGG GACAGCGTGT GCTTCCTGTT CCTGCTGCTC 
CAGCGCCGCG TGTTCCTCAG CCGCTACTTC CTGCACGTCA GTGCGGAGCT CCAGGCCACC 
GCCTTGCAGG CCTCCAGGTC AGGCCCCACC TCGGGCCCCC CGGGTTGTGC GGGCTCGTCC 
CCGCCACGGA GGCAGTGGTG GCGGCCCTGG CTGGACCACG CCACAGTTAT CCACTCGGGG 
GACTACTTCC TGTTCGAGTC TGACAGTGAG GAGGAGGAGG AGACCCCACC TGAGGACCCC 
AGGCCTTCGG CACAGAGCGC TTTCCAGCTG GCGTACCAGG CATGGGTGAC CAACGCGCAG 
ACGGTGCTGA GGAGGCAGCG GGAGGAGCAG GCGAGGCAGG AGCAGGCAGG GCAGCCGCCC 
GCGGGCCACA GCCACGTGAT GCAGCGGGTG CTGAGCGCCG TGCAGTTTCT GTGGGTGCTG 
GGACAGGCGC TGGTGGACGG GCTGACGGAC TGGCTGCGCA CCTTCACGCG GCACCACCGC 
GCCATGAGTG ACGTGTTGCG CGCCGAGCGC TACCTGTACA CGCAGGCGCT GCTCAGGGGC 
CAAGAGGTAC ACCGGGGCCT GCTGGACCAG CTGTATGCCA GGGGCGTGGA GGCCGCACCA 
CCAGGCTCCT CGGGGGCCCG AGACGCACCT GGCGCGGCGG CAAGTGGGCC GGGGGCACAG 
GAGCCACTGA GCGCCGCCAC GGAGGCCACC GGCGGCCACC TGAGCACGGG GTGCAACGCC 
CGCAGCAGCG GCGAGGACGC GACGGCCGAG CCTGGGGCTT CCCTGCACAG CTCCCGGGAG 
CTTCCCGCCG GTGCTTCCAC CCGGACCCGA ACCGCCAGCG AGCTGCTCCT CAACAGGCAC 
CTGCACATCC AGGAGCTGGA GGAGGCCCAG CAGTTCGAAG CGAGGCAGGG CCGGGCGCTG 
CGGCTGCTGC AGGCCGCCTA CCAGTGCGTG GCCGCCCACT CGGAGCTGCT CTGCTATTTC 
GTCATCATCC TCAACCACAT GGTCACCGCC TCGGCCACCT CCCTGGTGCT GCCCGTGCTC 
GTCTTCCTGT GGGCCATGCT GTCCATCCCG AGGCCCAGCA AGCGCTTCTG GATGACGGCC 
ATCGTCTTCA CCGAGGTGAC CGTGGTCACC AAGTACCTGT TCCAGTTTGG CTTCTTCCCC 
TGGAACACCC ACGCGGTGCT CCGGCGCTAC GAGAACAAGC CGTACTTCCC ACCACGGATC 
CTGGGCCTGG AGAAGACCGA CGGCTACGTC AAGTACGACC TAGTACAGCT CATGGCCTTG 
TTCTTCCACC GCTCCCAGCT GCTGTGCTAT GGCCTCTGGG ACCATGAAGG GGACCAACTC 
TCCAAGGACC ACGACCGCGG CACTGGGAAG AAGGGGGTTG AGGAGGAGCC GACGTCACCA 
GGACCCCAGG GAGAACCAGG GGTGGCTGGG GCCCTCGCCC AGTACCACAT GCAGGCGGAA 
GCAGGGGATG GGCCCCCAGA GCCTCCCGTG GAGCCCAAGC CCCGAGACCC AAAGCCGATC 
AGCCTGCGTT TCAGGAAGAG GAGAAGGGAG AGCACGGAGC CCGTGGAGCC CGTGGAGCCC 
GTGGAGCCCA CGGCTGTGGA AGGCGTGGCG GCGGCGCCGG GGAGCGAGAG GTGGAGTCGC 
CCCCGGGAAA GGGCGAGAGC CCTGGGGCTC CGACTGCAGA GCTGCTGCCT GGCCCTGGCC 
CAGAGCGTGT ACCGGCCCCT GCACTGCTTT TTCCACGACA TCCTGCACAC CCAGTACCGC 
GCGGCCACCG ACGTCTACGC CCTTATGTTC CTGGCCGACG TCGTTGACTT CATCATCATC 
ATCTTTGGCT TCTGGGCCTT TGGGAAACAC TCGGCAGCCA CAGACATCAC GTCCTCACTG 
TCAGACGACC AGGTGCCCGA GGCCTTCCTG GTCATGCTGC TCATCCAGTT CAGCACCATG 
GTCATCGACC GCGCCCTCTA CCTCCGCAAG ACCGTGCTGG GCAAGCTGGC CTTCCAGGTG 
GTCCTGGTGC TGGCCATCCA CCTCTGGATG TTCCTCATTC TGCCCGCGGT CACCGAGAGG 
ATGTTCAGCC AGAACGCAGT GGCCCAGCTG TGGTACTTCG TCAAGTGCGT CTACTTCGCC 
CTGTCCGCCT ACCAGATCCG CTGCGGCTAC CCCACCCGCA TCCTGGGCAA CTTCCTCACC 
AAGAAGTACA ACCACCTCAA CCTCTTCCTC TTCCAGGGTT TCCGGCTCGT GCCGTTCCTG 
GTGGAGCTGC GGGCCGTGAT GGACTGGGTG TGGACGGACA CCACGCTGTC CCTCTCCAGC 
TGGATGTGTG TGGAGGACAT CTACGCCAAC ATTTTCATCA TCAAGTGCAG CCGAGAGACC 
GAGAAGAAAT ACCCGCAGCC CAAGGGGCAG AAGAAGAAGA AGGTGGTCAA GTACGGCATG 
GGCGGGCTCA TCATCCTGTT CCTCGTGGCC ATCATCTGGT TCCCTCTGCT CTTTATGTCC 
CTGGTGCGCT CCGTGGTCGG CGTCGTCAAC CAGCCGATCG ACGTCACCGT CACCCTCAAG 
CTGGGTGGCT ACGAGCCCTT GTTCACCATG AGCGCGCAGC AGCCGTCCAT CGTGCCCTTC 
ACGCAACAGG CCTATGAGGA GCTGTCCAGA CACTTCGACC CGAACCCTCT GGCCATGCAG 
TTCATCAGCC AGTACAGCCC CGAGGACATC GTCACGGCGC AGATCGAGGG CAGCTCCGGG 
GCGCTGTGGC GCATCAGCCC GCCCAGCCGG GCCCAGATGA AGCGGGAGCT GTACAACGGC 
ACCGCCGACA TCACCCTGCG CTTCACCTGG AACTTCCAGA GGGACCTGGC CAAGGGCGGC 
ACCGTGGAAT ACACCAACGA GAAGCACACC CTGGACCTGG CTGCCAATAG CAGCGAGCGG 
AGGCAGCTGG CCAGCCTGCT GGAGGGCACC TCGGACCAGT CGGTGGTCAT CCCCAATCTC 
TTCCCCAAGT ACATCCGTGC TCCCAACGGG CCCGAGGCCA ACCCCGTGAA GCAGCTGCAG 
CCCAACGAGG AGGCCGACTA CCTCGGAGTG CGCATCCAGC TGCGGAGGGA GCGCGTGGGC 
TCGGGGGCCG CCGGCTTCCT GGAGTGGTGG GTCATCGAGC TGCAGGACTG CCAGGCCGAA 
TGCAACCTGC TCCCCATGGT CATCTTCAGC GACAAGGTCA GCCCGCCCAG CCTCGGCTTC 
CTGGCCGGCT ACGGGATCAT GGGGCTCTAC GTGTCCATCG TGCTGGTCAT CGGCAAGTTC 
GTGCGTGGCT TCTTCAGCGA GATCTCCCAC TCCATCATGT TTGAGGAGCT GCCCTGCGTG 
GACCGCATCC TCAAGCTCTG CCAGGACATC TTCCTGGTGC GGGAGACGCG CGAGCTGGAG 
CTGGAGGAGG AGCTCTACGC CAAGCTCATC TTCCTGTACC GCTCGCCCGA GACCATGATC 
AAGTGGACCC GCGAGAAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019674531.1
CDS	16..6279
Translation	MIPETLEAGLLSWAWAAPRGPSCCPSDGKTGCHWLCCSSVCLLVLCQALAGNACLCRFNALSLVYLLFLLLLPWFPGPSPHSVPGHTGRLLRALLLFSLLFLAAHLAFQICLHTVPRLDQLLGPSCSPWQILSQHVGVTRLDLKDIPNAVRLVAPDLGVLVVSAVCLGLCGRLAREARRSQPAQEQDDEDREADAGSPAGPRGAPVPAPKQRSRLGSISILREARGEIAGLHSWLPGDLPCAQSRMGVGAILSDPSFHSLGNQDVEWVHPRLSEPRDVSPLHGLGHLIMDQSYVCALIAMMVRSGWPGQARPGGPCCWFPRDVLGGRMAACKGLIPREGTEAAGFGREGPEQGDGLSRPLGHGVPAVPGVPPAFGSRGQHIGRAPGEAFLGAHPRQDAVSGTPLLQEEEEVAREEGLSEAGPQQAVQVPEAAASKWGLVAERLLDLADRFSEVLTRVQVLVRRLLELHVLKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCIIIVCKMLYQLKVVSPHEYSSNCTEPLLNSTNLQKTEIKQSLLYRGPVDPANWFGVRKGFPNLGYIQNHLLVLLLLVFEAIVYRRQEHHRRQHQPAPLPAQAVCADGTRQRLDQDLLSCFKYFVNFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAILTRRHRGAIARLWPNYCLFLALFFLYQYLLCLGIPPALCLDYPWRWSQAIPMNSALIKWLYLPDFFRTPNSTNLISERLRPGPRGGGAPHRLPWAEAAGRRWGRPGPAVTPPSGRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTSLLQKHTRARLLLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSSFCWVIQLFSLVCTVKGYYDPKEMLSRDRDCLLPVEEAGVLWDSVCFLFLLLQRRVFLSRYFLHVSAELQATALQASRSGPTSGPPGCAGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEETPPEDPRPSAQSAFQLAYQAWVTNAQTVLRRQREEQARQEQAGQPPAGHSHVMQRVLSAVQFLWVLGQALVDGLTDWLRTFTRHHRAMSDVLRAERYLYTQALLRGQEVHRGLLDQLYARGVEAAPPGSSGARDAPGAAASGPGAQEPLSAATEATGGHLSTGCNARSSGEDATAEPGASLHSSRELPAGASTRTRTASELLLNRHLHIQELEEAQQFEARQGRALRLLQAAYQCVAAHSELLCYFVIILNHMVTASATSLVLPVLVFLWAMLSIPRPSKRFWMTAIVFTEVTVVTKYLFQFGFFPWNTHAVLRRYENKPYFPPRILGLEKTDGYVKYDLVQLMALFFHRSQLLCYGLWDHEGDQLSKDHDRGTGKKGVEEEPTSPGPQGEPGVAGALAQYHMQAEAGDGPPEPPVEPKPRDPKPISLRFRKRRRESTEPVEPVEPVEPTAVEGVAAAPGSERWSRPRERARALGLRLQSCCLALAQSVYRPLHCFFHDILHTQYRAATDVYALMFLADVVDFIIIIFGFWAFGKHSAATDITSSLSDDQVPEAFLVMLLIQFSTMVIDRALYLRKTVLGKLAFQVVLVLAIHLWMFLILPAVTERMFSQNAVAQLWYFVKCVYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSSWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKVVKYGMGGLIILFLVAIIWFPLLFMSLVRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTQQAYEELSRHFDPNPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYTNEKHTLDLAANSSERRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRRERVGSGAAGFLEWWVIELQDCQAECNLLPMVIFSDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Target ORF information:

RefSeq Version	XM_019818972.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus piezo type mechanosensitive ion channel component 1 (PIEZO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019818972.1

ORF Insert Sequence:

ATGATACCTG AGACTCTGGA GGCTGGTCTC CTGTCCTGGG CGTGGGCAGC TCCCCGGGGA 
CCCTCCTGCT GTCCCTCTGA TGGCAAGACC GGCTGCCATT GGCTCTGCTG CTCTTCTGTG 
TGCCTCCTCG TCCTCTGCCA GGCTTTGGCT GGAAATGCCT GCCTGTGCCG CTTCAACGCC 
CTGTCTCTGG TCTACCTGCT GTTTCTGCTG CTGTTGCCCT GGTTCCCGGG CCCCTCTCCG 
CACAGCGTCC CAGGTCACAC TGGTCGCCTC CTCCGAGCCC TGCTGCTTTT CAGCCTCCTC 
TTCCTGGCAG CCCACCTGGC CTTCCAGATA TGCCTGCACA CCGTGCCCCG CCTGGACCAG 
CTCCTGGGGC CAAGCTGCAG CCCCTGGCAG ATCCTGTCCC AGCACGTAGG GGTGACGAGG 
CTGGACCTGA AGGACATCCC CAATGCCGTC CGGCTGGTGG CCCCTGACCT GGGTGTCCTG 
GTGGTCTCAG CCGTGTGCCT CGGCCTCTGT GGGCGCCTGG CACGGGAAGC CCGGCGGAGC 
CAGCCCGCCC AGGAGCAGGA CGATGAGGAC AGGGAAGCGG ACGCCGGCTC CCCGGCGGGG 
CCTCGCGGAG CCCCCGTGCC GGCCCCCAAG CAGAGGTCTC GGCTGGGCTC GATTTCCATT 
TTGCGCGAAG CAAGAGGCGA AATCGCCGGC CTCCACAGCT GGCTCCCCGG GGACTTGCCC 
TGCGCACAGA GCCGGATGGG AGTCGGGGCC ATTTTGTCCG ACCCGTCCTT TCACAGTCTT 
GGAAACCAAG ATGTGGAGTG GGTGCACCCC AGGCTGTCGG AGCCTAGAGA CGTGTCTCCC 
CTGCACGGTC TGGGCCATCT CATCATGGAC CAGAGCTACG TGTGTGCCCT CATCGCCATG 
ATGGTAAGGA GCGGCTGGCC TGGGCAGGCC CGTCCGGGGG GCCCTTGCTG CTGGTTCCCT 
CGTGATGTTC TGGGGGGGCG GATGGCGGCC TGCAAGGGCC TCATCCCCCG GGAAGGGACG 
GAGGCTGCTG GGTTCGGACG GGAGGGCCCG GAGCAGGGCG ACGGCCTCAG CAGACCCCTC 
GGACACGGCG TGCCCGCGGT GCCGGGTGTT CCTCCGGCCT TCGGGTCACG GGGGCAGCAC 
ATCGGCCGGG CCCCAGGGGA GGCCTTTCTT GGGGCCCACC CTAGGCAGGA CGCGGTCAGC 
GGGACCCCGC TGCTGCAGGA GGAGGAGGAG GTGGCCAGGG AGGAGGGACT GAGCGAGGCC 
GGCCCCCAGC AGGCCGTGCA GGTCCCCGAA GCTGCCGCCA GCAAGTGGGG CCTGGTGGCC 
GAGCGGCTGC TGGACCTGGC TGACCGCTTC TCCGAGGTCC TCACCCGCGT GCAGGTGTTG 
GTGCGACGCC TGCTGGAGCT GCACGTCCTC AAGCTGGTGG CCCTGTACAC TGTGTGGGTG 
GCCCTGAAGG AGGTGTCGGT GATGAACCTG CTTCTGGTCG TGCTGTGGGC CTTCGCCCTG 
CCCTACCCCC GCTTCCGGCC CATGGCCTCC TGCCTGTCCA CCGTGTGGAC CTGTATCATC 
ATCGTGTGCA AGATGCTGTA TCAGCTGAAG GTCGTCAGCC CGCACGAATA CTCCAGCAAC 
TGCACCGAGC CCCTCCTCAA CAGCACCAAC CTGCAGAAAA CGGAGATCAA GCAGTCCCTG 
CTGTACCGGG GACCCGTCGA CCCTGCCAAC TGGTTTGGGG TCCGGAAGGG CTTCCCGAAC 
CTGGGCTACA TCCAGAACCA CCTGCTGGTC CTGCTGCTGC TGGTGTTCGA GGCCATTGTT 
TACCGGCGCC AGGAGCACCA CCGTCGGCAG CACCAGCCGG CCCCGCTGCC TGCCCAGGCC 
GTCTGTGCCG ACGGCACCCG CCAGCGGCTG GACCAAGATC TTCTCAGCTG CTTCAAGTAC 
TTCGTCAACT TCTTTTTCTA CAAATTCGGG CTGGAGATCT GCTTCCTGAT GGCCGTGAAC 
GTGATTGGGC AGCGCATGAA TTTCATGGTC ATCCTGCACG GCTGCTGGCT GGTGGCCATT 
CTTACCCGCC GGCACCGCGG AGCCATCGCC CGCCTCTGGC CCAACTACTG TCTGTTCCTG 
GCGTTGTTCT TCCTGTACCA GTACCTGCTC TGCCTGGGCA TCCCCCCGGC CCTGTGCCTC 
GACTACCCGT GGCGGTGGAG CCAGGCCATC CCCATGAACT CCGCGCTCAT CAAGTGGCTG 
TACCTGCCCG ACTTCTTCAG AACCCCCAAC TCCACCAACC TCATCAGTGA GCGCCTGCGG 
CCGGGCCCCA GAGGCGGGGG CGCGCCTCAC CGGCTCCCCT GGGCCGAGGC TGCCGGGAGG 
AGGTGGGGCC GCCCGGGCCC CGCGGTGACG CCCCCGTCTG GCAGGTCCTA CCTCGACATG 
CTGAAGGTGG CCGTCTTCCG CTACCTCTTC TGGCTGGTGC TGGTGGTGGT GTTCGTCACG 
GGAGCCACCC GCATCAGTAT CTTCGGGCTG GGCTACCTGC TGGCCTGCTT CTACCTGCTG 
CTCTTCGGCA CCAGCCTGTT GCAGAAGCAC ACGCGTGCCC GCCTCCTGCT GTGGGACTGC 
CTCATTCTGT ACAACGTCAC CGTCATCATC TCCAAGAACA TGCTTTCGCT CCTGTCCTGC 
GTGTTCGTGG AGCAAATGCA GAGCAGCTTC TGCTGGGTCA TCCAGCTCTT CAGCCTCGTG 
TGCACCGTCA AAGGCTACTA TGACCCCAAG GAGATGCTGA GCCGGGACCG GGACTGCCTG 
CTGCCCGTGG AGGAGGCCGG CGTGCTCTGG GACAGCGTGT GCTTCCTGTT CCTGCTGCTC 
CAGCGCCGCG TGTTCCTCAG CCGCTACTTC CTGCACGTCA GTGCGGAGCT CCAGGCCACC 
GCCTTGCAGG CCTCCAGGTC AGGCCCCACC TCGGGCCCCC CGGGTTGTGC GGGCTCGTCC 
CCGCCACGGA GGCAGTGGTG GCGGCCCTGG CTGGACCACG CCACAGTTAT CCACTCGGGG 
GACTACTTCC TGTTCGAGTC TGACAGTGAG GAGGAGGAGG AGACCCCACC TGAGGACCCC 
AGGCCTTCGG CACAGAGCGC TTTCCAGCTG GCGTACCAGG CATGGGTGAC CAACGCGCAG 
ACGGTGCTGA GGAGGCAGCG GGAGGAGCAG GCGAGGCAGG AGCAGGCAGG GCAGCCGCCC 
GCGGGCCACA GCCACGTGAT GCAGCGGGTG CTGAGCGCCG TGCAGTTTCT GTGGGTGCTG 
GGACAGGCGC TGGTGGACGG GCTGACGGAC TGGCTGCGCA CCTTCACGCG GCACCACCGC 
GCCATGAGTG ACGTGTTGCG CGCCGAGCGC TACCTGTACA CGCAGGCGCT GCTCAGGGGC 
CAAGAGGTAC ACCGGGGCCT GCTGGACCAG CTGTATGCCA GGGGCGTGGA GGCCGCACCA 
CCAGGCTCCT CGGGGGCCCG AGACGCACCT GGCGCGGCGG CAAGTGGGCC GGGGGCACAG 
GAGCCACTGA GCGCCGCCAC GGAGGCCACC GGCGGCCACC TGAGCACGGG GTGCAACGCC 
CGCAGCAGCG GCGAGGACGC GACGGCCGAG CCTGGGGCTT CCCTGCACAG CTCCCGGGAG 
CTTCCCGCCG GTGCTTCCAC CCGGACCCGA ACCGCCAGCG AGCTGCTCCT CAACAGGCAC 
CTGCACATCC AGGAGCTGGA GGAGGCCCAG CAGTTCGAAG CGAGGCAGGG CCGGGCGCTG 
CGGCTGCTGC AGGCCGCCTA CCAGTGCGTG GCCGCCCACT CGGAGCTGCT CTGCTATTTC 
GTCATCATCC TCAACCACAT GGTCACCGCC TCGGCCACCT CCCTGGTGCT GCCCGTGCTC 
GTCTTCCTGT GGGCCATGCT GTCCATCCCG AGGCCCAGCA AGCGCTTCTG GATGACGGCC 
ATCGTCTTCA CCGAGGTGAC CGTGGTCACC AAGTACCTGT TCCAGTTTGG CTTCTTCCCC 
TGGAACACCC ACGCGGTGCT CCGGCGCTAC GAGAACAAGC CGTACTTCCC ACCACGGATC 
CTGGGCCTGG AGAAGACCGA CGGCTACGTC AAGTACGACC TAGTACAGCT CATGGCCTTG 
TTCTTCCACC GCTCCCAGCT GCTGTGCTAT GGCCTCTGGG ACCATGAAGG GGACCAACTC 
TCCAAGGACC ACGACCGCGG CACTGGGAAG AAGGGGGTTG AGGAGGAGCC GACGTCACCA 
GGACCCCAGG GAGAACCAGG GGTGGCTGGG GCCCTCGCCC AGTACCACAT GCAGGCGGAA 
GCAGGGGATG GGCCCCCAGA GCCTCCCGTG GAGCCCAAGC CCCGAGACCC AAAGCCGATC 
AGCCTGCGTT TCAGGAAGAG GAGAAGGGAG AGCACGGAGC CCGTGGAGCC CGTGGAGCCC 
GTGGAGCCCA CGGCTGTGGA AGGCGTGGCG GCGGCGCCGG GGAGCGAGAG GTGGAGTCGC 
CCCCGGGAAA GGGCGAGAGC CCTGGGGCTC CGACTGCAGA GCTGCTGCCT GGCCCTGGCC 
CAGAGCGTGT ACCGGCCCCT GCACTGCTTT TTCCACGACA TCCTGCACAC CCAGTACCGC 
GCGGCCACCG ACGTCTACGC CCTTATGTTC CTGGCCGACG TCGTTGACTT CATCATCATC 
ATCTTTGGCT TCTGGGCCTT TGGGAAACAC TCGGCAGCCA CAGACATCAC GTCCTCACTG 
TCAGACGACC AGGTGCCCGA GGCCTTCCTG GTCATGCTGC TCATCCAGTT CAGCACCATG 
GTCATCGACC GCGCCCTCTA CCTCCGCAAG ACCGTGCTGG GCAAGCTGGC CTTCCAGGTG 
GTCCTGGTGC TGGCCATCCA CCTCTGGATG TTCCTCATTC TGCCCGCGGT CACCGAGAGG 
ATGTTCAGCC AGAACGCAGT GGCCCAGCTG TGGTACTTCG TCAAGTGCGT CTACTTCGCC 
CTGTCCGCCT ACCAGATCCG CTGCGGCTAC CCCACCCGCA TCCTGGGCAA CTTCCTCACC 
AAGAAGTACA ACCACCTCAA CCTCTTCCTC TTCCAGGGTT TCCGGCTCGT GCCGTTCCTG 
GTGGAGCTGC GGGCCGTGAT GGACTGGGTG TGGACGGACA CCACGCTGTC CCTCTCCAGC 
TGGATGTGTG TGGAGGACAT CTACGCCAAC ATTTTCATCA TCAAGTGCAG CCGAGAGACC 
GAGAAGAAAT ACCCGCAGCC CAAGGGGCAG AAGAAGAAGA AGGTGGTCAA GTACGGCATG 
GGCGGGCTCA TCATCCTGTT CCTCGTGGCC ATCATCTGGT TCCCTCTGCT CTTTATGTCC 
CTGGTGCGCT CCGTGGTCGG CGTCGTCAAC CAGCCGATCG ACGTCACCGT CACCCTCAAG 
CTGGGTGGCT ACGAGCCCTT GTTCACCATG AGCGCGCAGC AGCCGTCCAT CGTGCCCTTC 
ACGCAACAGG CCTATGAGGA GCTGTCCAGA CACTTCGACC CGAACCCTCT GGCCATGCAG 
TTCATCAGCC AGTACAGCCC CGAGGACATC GTCACGGCGC AGATCGAGGG CAGCTCCGGG 
GCGCTGTGGC GCATCAGCCC GCCCAGCCGG GCCCAGATGA AGCGGGAGCT GTACAACGGC 
ACCGCCGACA TCACCCTGCG CTTCACCTGG AACTTCCAGA GGGACCTGGC CAAGGGCGGC 
ACCGTGGAAT ACACCAACGA GAAGCACACC CTGGACCTGG CTGCCAATAG CAGCGAGCGG 
AGGCAGCTGG CCAGCCTGCT GGAGGGCACC TCGGACCAGT CGGTGGTCAT CCCCAATCTC 
TTCCCCAAGT ACATCCGTGC TCCCAACGGG CCCGAGGCCA ACCCCGTGAA GCAGCTGCAG 
CCCAACGAGG AGGCCGACTA CCTCGGAGTG CGCATCCAGC TGCGGAGGGA GCGCGTGGGC 
TCGGGGGCCG CCGGCTTCCT GGAGTGGTGG GTCATCGAGC TGCAGGACTG CCAGGCCGAA 
TGCAACCTGC TCCCCATGGT CATCTTCAGC GACAAGGTCA GCCCGCCCAG CCTCGGCTTC 
CTGGCCGGCT ACGGGATCAT GGGGCTCTAC GTGTCCATCG TGCTGGTCAT CGGCAAGTTC 
GTGCGTGGCT TCTTCAGCGA GATCTCCCAC TCCATCATGT TTGAGGAGCT GCCCTGCGTG 
GACCGCATCC TCAAGCTCTG CCAGGACATC TTCCTGGTGC GGGAGACGCG CGAGCTGGAG 
CTGGAGGAGG AGCTCTACGC CAAGCTCATC TTCCTGTACC GCTCGCCCGA GACCATGATC 
AAGTGGACCC GCGAGAAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO1 ( XM_023244898.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023100666.1 Felis catus piezo type mechanosensitive ion channel component 1 (PIEZO1), mRNA.

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CloneID	OFb65504
Clone ID Related Accession (Same CDS sequence)	XM_023244898.1
Accession Version	XM_023244898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	LOW QUALITY PROTEIN: piezo-type mechanosensitive ion channel component 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018737.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTGGGCCT GTCCCTGCCC GAGCTTGGGG TCTCCTGGCC CGGCTCCCAG ACTCCGGTGG 
GATGTGGGTC CCCCAGGACG GGCGGAAGAA GGCCCTGTGC CAGCCTCGCC CCTGCCCTCT 
CTGCCCGCAG GTCACACTGG TCGCCTCCTC CGAGCCCTGC TGCTTTTCAG CCTCCTCTTC 
CTGGCAGCCC ACCTGGCCTT CCAGATATGC CTGCACACCG TGCCCCGCCT GGACCAGCTC 
CTGGGGCCAA GCTGCAGCCC CTGGCAGATC CTGTCCCAGC ACGTAGGGGT GACGAGGCTG 
GACCTGAAGG ACATCCCCAA TGCCGTCCGG CTGGTGGCCC CTGACCTGGG TGTCCTGGTG 
GTCTCAGCCG TGTGCCTCGG CCTCTGTGGG CGCCTGGCAC GGGAAGCCCG GCGGAGCCAG 
CCCGCCCAGG AGCAGGACGA TGAGGACAGG GAAGCGGACG CCGGCTCCCC GGCGGGGCCT 
CGCGGAGCCC CCGTGCCGGC CCCCAAGCAG AGGTCTCGGC TGGCCACCCG GTTCCAGGTC 
ACGGCCCACT GGCTGCTGGT GGCCGCCGGA CGGACGCTGG CCATCCTGCT GCTGGCGCTG 
GCAGGCATCG CCCAGCCCTC GGCCTTCTCC GGCGTCTACT TCCTGGTCTT CCTGGCCACC 
TGCACCTGGT GGGCCTGCCA CTTTCCCATC GGCCCCCTGG GCTCCAGCAC GCTCTGCGTC 
ATGGTGGGCT GCTTCAGCGC CGGCCACCTC ATCTGCGTCT ACTGCTACCA GACGCCCTTC 
GCCCAGGCCG TGCTGCCCCC TGCCGGCATC TGGGCCAGGG TGTTCGGCCT CAAGGCCTTG 
GTGGCCTACA GCAACTGCTC CGGTCCCAAC GTGCTGGTCC TCGATACCAG CCATGACTGG 
CCCGTGTACG TGAGCCCCGG CGTCCTGCTG CTCCTCTACT ACACCGTGAC CTCACTCCTC 
AAGCTGTGCA CACACCAGCC CCTGGACCAG AGGAAGGAGG CGGCCGGGCG CGACGAGGAG 
CAGGAGGTGG AACTGACTCA CGTGGATCAG TGGCCCCAGG GCCGGGCCGG GGCTGCGGCC 
ACCAAGGAGG AGGGCGCCGC CCAGCGCGTG CCGCCCACGA CCACGGGACC AGACTGGGAG 
ACCGACAGCT GCACCGTGCA CGACCTGACC GGCCACAGCC CCGTCCGGCA GCGCCCCCTG 
CACCCCAGGC TGTCGGAGCC TAGAGACGTG TCTCCCCTGC ACGGTCTGGG CCATCTCATC 
ATGGACCAGA GCTACGTGTG TGCCCTCATC GCCATGATGG TGTGGAGTAT CACCTACCAC 
AGCTGGCTGA CCTTCGTGCT GCTGCTCTGG GCCTGCCTCA TCTGGACGGT GCGCAGTCGT 
CACCAGCTGG CCATGCTCTG TTCCCCCTTC ATCCTGCTCT ACGGGCTGGC GCTGTGCGGG 
CTCCGCTACG TGTGGGCCAT GGACCTGCGT CCCGAGCTGC CCACCACCCT GGGCCCCGTC 
AGCCTGCGTC AGCTGGGGCT GGAGCACACC CGCTACCCTT GCCTGGACCT TGGTGCCATG 
CTGCTGTACA ACCTGACCTT CTGGCTCCTG CTGCGCCAGT TCGTGAAGGA GAAGCTGCTA 
AGGAAGGCCA AGAGCGCTGC CGGCCTGCTG GGGGTCGCCG TGGCCGACCC AGAGCCCACG 
CGGACACGGA CGCTGCTCCG GAGCCTGGGC GAGCTGGTCA CGGGCCTGTA CGCCAAGTAC 
TGGATCTACG TGTGCGCCGG CATGTTCGTC GTGGTCAGCT TTGCCGGCCG CCTCGTCGTC 
TACAAGATCG TGTACATGCT CCTCTTCCTG CTGTGCCTCA CGCTCTTCCA GGTCTACTAC 
AGCCTGTGGA GGAAGCTGCT CAAGGCGTTC TGGTGGCTCG TGGTAGCCTA CACCATGCTG 
GTGCTCAGCG CCGTCTACAC CTTCCAGTTC CAGGACTTCC CCACCTACTG GCGCAACCTG 
ACCGGCCTCA CGGACGAGCA GCTGGCGGAC CTGGGTCTGG AGCAGTTCAG CGTGTCCGAG 
CTCTTCTCCA GCATCCTGGT CCCCGGCTTC TTCCTGCTGG CCTGCATTCT GCAGCTGCAT 
TATTTCCACC AGCCCTTCAT GCGGCTCACC GACCTGGGGC GCGCGCCCCC GCCCGGCGCC 
CACCTCCCGC GCTGGGCTCG AAGGCAGGAC GCGGTCAGCG GGACCCCGCT GCTGCAGGAG 
GAGGAGGAGG TGGCCAGGGA GGAGGGACTG AGCGAGGCCG GCCCCCAGCA GGCCGTGCAG 
GTCCCCGAAG CTGCCGCCAG CAAGTGGGGC CTGGTGGCCG AGCGGCTGCT GGACCTGGCT 
GACCGCTTCT CCGAGGTCCT CACCCGCGTG CAGGTGTTGG TGCGACGCCT GCTGGAGCTG 
CACGTCCTCA AGCTGGTGGC CCTGTACACT GTGTGGGTGG CCCTGAAGGA GGTGTCGGTG 
ATGAACCTGC TTCTGGTCGT GCTGTGGGCC TTCGCCCTGC CCTACCCCCG CTTCCGGCCA 
NTGGCCTCCT GCCTGTCCAC CGTGTGGACC TGTATCATCA TCGTGTGCAA GATGCTGTAT 
CAGCTGAAGG TCGTCAGCCC GCACGAATAC TCCAGCAACT GCACCGAGCC CCTCCTCAAC 
AGCACCAACC TGCAGAAAAC GGAGATCAAG CAGTCCCTGC TGTACCGGGG ACCCGTCGAC 
CCTGCCAACT GGTTTGGGGT CCGGAAGGGC TTCCCGAACC TGGGCTACAT CCAGAACCAC 
CTGCTGGTCC TGCTGCTGCT GGTGTTCGAG GCCATTGTTT ACCGGCGCCA GGAGCACCAC 
CGTCGGCAGC ACCAGCCGGC CCCGCTGCCT GCCCAGGCCG TCTGTGCCGA CGGCACCCGC 
CAGCGGCTGG ACCAAGATCT TCTCAGCTGC TTCAAGTACT TCGTCAACTT CTTTTTCTAC 
AAATTCGGGC TGGAGATCTG CTTCCTGATG GCCGTGAACG TGATTGGGCA GCGCATGAAT 
TTCATGGTCA TCCTGCACGG CTGCTGGCTG GTGGCCATTC TTACCCGCCG GCACCGCGGA 
GCCATCGCCC GCCTCTGGCC CAACTACTGT CTGTTCCTGG CGTTGTTCTT CCTGTACCAG 
TACCTGCTCT GCCTGGGCAT CCCCCCGGCC CTGTGCCTCG ACTACCCGTG GCGGTGGAGC 
CAGGCCATCC CCATGAACTC CGCGCTCATC AAGTGGCTGT ACCTGCCCGA CTTCTTCAGA 
ACCCCCAACT CCACCAACCT CATCAGTGAC TTCCTCCTGC TGCTGTGCGC CTCCCAGCAG 
TGGCAGGTGT TCGCAGCCGA GCGCACGGAA GAGTGGCAGC ACGTGGCCGG CACCAACACT 
GACCGCCTGG AGGTGCCGCA GGGCGAGCCC GACCCCGTGC CCAACTTCAT TTACTGCAGG 
TCCTACCTCG ACATGCTGAA GGTGGCCGTC TTCCGCTACC TCTTCTGGCT GGTGCTGGTG 
GTGGTGTTCG TCACGGGAGC CACCCGCATC AGTATCTTCG GGCTGGGCTA CCTGCTGGCC 
TGCTTCTACC TGCTGCTCTT CGGCACCAGC CTGTTGCAGA AGCACACGCG TGCCCGCCTC 
CTGCTGTGGG ACTGCCTCAT TCTGTACAAC GTCACCGTCA TCATCTCCAA GAACATGCTT 
TCGCTCCTGT CCTGCGTGTT CGTGGAGCAA ATGCAGAGCA GCTTCTGCTG GGTCATCCAG 
CTCTTCAGCC TCGTGTGCAC CGTCAAAGGC TACTATGACC CCAAGGAGAT GCTGAGCCGG 
GACCGGGACT GCCTGCTGCC CGTGGAGGAG GCCGGCGTGC TCTGGGACAG CGTGTGCTTC 
CTGTTCCTGC TGCTCCAGCG CCGCGTGTTC CTCAGCCGCT ACTTCCTGCA CGTCAGTGCG 
GAGCTCCAGG CCACCGCCTT GCAGGCCTCC AGGGGCTTCG CGCTGTACAA CGCGGCCAAC 
CTCAAGAGCA TCGAGCTCCA TCGCAAGGCG GAGGAGAAGT CCCTGGCCCA GCTGAAGAGA 
CAGATGGAGC GCATCCGCGC CAAACAGGAG AAGCACAGGC AGAGCCGGGC CGGCCGCAGC 
CGCCCCCAGG AGCCCCAGGG CCCCCCCCAG GAGCCGGGGC CTGGCAGTCC ACGGGGCTCG 
TCCCCGCCAC GGAGGCAGTG GTGGCGGCCC TGGCTGGACC ACGCCACAGT TATCCACTCG 
GGGGACTACT TCCTGTTCGA GTCTGACAGT GAGGAGGAGG AGGAGACCCC ACCTGAGGAC 
CCCAGGCCTT CGGCACAGAG CGCTTTCCAG CTGGCGTACC AGGCATGGGT GACCAACGCG 
CAGACGGTGC TGAGGAGGCA GCGGGAGGAG CAGGCGAGGC AGGAGCAGGC AGGGCAGCCG 
CCCGCGGGAG GCGGCCCGGG CGCAGAGGCG GAGCCGGCGG AGGGCCCAGA CCAAGAGGCG 
GCTGGCCACA GCCACGTGAT GCAGCGGGTG CTGAGCGCCG TGCAGTTTCT GTGGGTGCTG 
GGACAGGCGC TGGTGGACGG GCTGACGGAC TGGCTGCGCA CCTTCACGCG GCACCACCGC 
GCCATGAGTG ACGTGTTGCG CGCCGAGCGC TACCTGTACA CGCAGGCGCT GCTCAGGGGC 
CAAGAGGTAC ACCGGGGCCT GCTGGACCAG CTGTATGCCA GGGGCGTGGA GGCCGCACCA 
CCAGGCTCCT CGGGGGCCCG AGACGCACCT GGCGCGGCGG CAAGTGGGCC GGGGGCACAG 
GAGCCACTGA GCGCCGCCAC GGAGGCCACC GGCGGCCACC TGAGCACGGG GTGCAACGCC 
CGCAGCAGCG GCGAGGACGC GACGGCCGAG CCTGGGGCTT CCCTGCACAG CTCCCGGGAG 
CTTCCCGCCG GTGCTTCCAC CCGGACCCGA ACCGCCAGCG AGCTGCTCCT CAACAGGCAC 
CTGCACATCC AGGAGCTGGA GGAGGCCCAG CAGTTCGAAG CGAGGCAGGG CCGGGCGCTG 
CGGCTGCTGC AGGCCGCCTA CCAGTGCGTG GCCGCCCACT CGGAGCTGCT CTGCTATTTC 
GTCATCATCC TCAACCACAT GGTCACCGCC TCGGCCACCT CCCTGGTGCT GCCCGTGCTC 
GTCTTCCTGT GGGCCATGCT GTCCATCCCG AGGCCCAGCA AGCGCTTCTG GATGACGGCC 
ATCGTCTTCA CCGAGGTGAC CGTGGTCACC AAGTACCTGT TCCAGTTTGG CTTCTTCCCC 
TGGAACACCC ACGCGGTGCT CCGGCGCTAC GAGAACAAGC CGTACTTCCC ACCACGGATC 
CTGGGCCTGG AGAAGACCGA CGGCTACGTC AAGTACGACC TAGTACAGCT CATGGCCTTG 
TTCTTCCACC GCTCCCAGCT GCTGTGCTAT GGCCTCTGGG ACCATGAAGG GGACCAACTC 
TCCAAGGACC ACGACCGCGG CACTGGGAAG AAGGGGGTTG AGGAGGAGCC GACGTCACCA 
GGACCCCAGG GAGAACCAGG GGTGGCTGGG GCCCTCGCCC AGTACCACAT GCAGGCGGAA 
GCAGGGGATG GGCCCCCAGA GCCTCCCGTG GAGCCCAAGC CCCGAGACCC AAAGCCGATC 
AGCCTGCGTT TCAGGAAGAG GAGAAGGGAG AGCACGGAGC CCGTGGAGCC CGTGGAGCCC 
GTGGAGCCCA CGGCTGTGGA AGGCGTGGCG GCGGCGCCGG GGAGCGAGAG GTGGAGTCGC 
CCCCGGGAAA GGGCGAGAGC CCTGGGGCTC CGACTGCAGA GCTGCTGCCT GGCCCTGGCC 
CAGAGCGTGT ACCGGCCCCT GCACTGCTTT TTCCACGACA TCCTGCACAC CCAGTACCGC 
GCGGCCACCG ACGTCTACGC CCTTATGTTC CTGGCCGACG TCGTTGACTT CATCATCATC 
ATCTTTGGCT TCTGGGCCTT TGGGAAACAC TCGGCAGCCA CAGACATCAC GTCCTCACTG 
TCAGACGACC AGGTGCCCGA GGCCTTCCTG GTCATGCTGC TCATCCAGTT CAGCACCATG 
GTCATCGACC GCGCCCTCTA CCTCCGCAAG ACCGTGCTGG GCAAGCTGGC CTTCCAGGTG 
GTCCTGGTGC TGGCCATCCA CCTCTGGATG TTCCTCATTC TGCCCGCGGT CACCGAGAGG 
ATGTTCAGCC AGAACGCAGT GGCCCAGCTG TGGTACTTCG TCAAGTGCGT CTACTTCGCC 
CTGTCCGCCT ACCAGATCCG CTGCGGCTAC CCCACCCGCA TCCTGGGCAA CTTCCTCACC 
AAGAAGTACA ACCACCTCAA CCTCTTCCTC TTCCAGGGTT TCCGGCTCGT GCCGTTCCTG 
GTGGAGCTGC GGGCCGTGAT GGACTGGGTG TGGACGGACA CCACGCTGTC CCTCTCCAGC 
TGGATGTGTG TGGAGGACAT CTACGCCAAC ATTTTCATCA TCAAGTGCAG CCGAGAGACC 
GAGAAGAAAT ACCCGCAGCC CAAGGGGCAG AAGAAGAAGA AGGTGGTCAA GTACGGCATG 
GGCGGGCTCA TCATCCTGTT CCTCGTGGCC ATCATCTGGT TCCCTCTGCT CTTTATGTCC 
CTGGTGCGCT CCGTGGTCGG CGTCGTCAAC CAGCCGATCG ACGTCACCGT CACCCTCAAG 
CTGGGTGGCT ACGAGCCCTT GTTCACCATG AGCGCGCAGC AGCCGTCCAT CGTGCCCTTC 
ACGCAACAGG CCTATGAGGA GCTGTCCAGA CACTTCGACC CGAACCCTCT GGCCATGCAG 
TTCATCAGCC AGTACAGCCC CGAGGACATC GTCACGGCGC AGATCGAGGG CAGCTCCGGG 
GCGCTGTGGC GCATCAGCCC GCCCAGCCGG GCCCAGATGA AGCGGGAGCT GTACAACGGC 
ACCGCCGACA TCACCCTGCG CTTCACCTGG AACTTCCAGA GGGACCTGGC CAAGGGCGGC 
ACCGTGGAAT ACACCAACGA GAAGCACACC CTGGACCTGG CTGCCAATAG CAGCGAGCGG 
AGGCAGCTGG CCAGCCTGCT GGAGGGCACC TCGGACCAGT CGGTGGTCAT CCCCAATCTC 
TTCCCCAAGT ACATCCGTGC TCCCAACGGG CCCGAGGCCA ACCCCGTGAA GCAGCTGCAG 
CCCAACGAGG AGGCCGACTA CCTCGGAGTG CGCATCCAGC TGCGGAGGGA GCGCGTGGGC 
TCGGGGGCCG CCGGCTTCCT GGAGTGGTGG GTCATCGAGC TGCAGGACTG CCAGGCCGAA 
TGCAACCTGC TCCCCATGGT CATCTTCAGC GACAAGGTCA GCCCGCCCAG CCTCGGCTTC 
CTGGCCGGCT ACGGGATCAT GGGGCTCTAC GTGTCCATCG TGCTGGTCAT CGGCAAGTTC 
GTGCGTGGCT TCTTCAGCGA GATCTCCCAC TCCATCATGT TTGAGGAGCT GCCCTGCGTG 
GACCGCATCC TCAAGCTCTG CCAGGACATC TTCCTGGTGC GGGAGACGCG CGAGCTGGAG 
CTGGAGGAGG AGCTCTACGC CAAGCTCATC TTCCTGTACC GCTCGCCCGA GACCATGATC 
AAGTGGACCC GCGAGAAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023100666.1
CDS	16..7539
Translation	MWACPCPSLGSPGPAPRLRWDVGPPGRAEEGPVPASPLPSLPAGHTGRLLRALLLFSLLFLAAHLAFQICLHTVPRLDQLLGPSCSPWQILSQHVGVTRLDLKDIPNAVRLVAPDLGVLVVSAVCLGLCGRLAREARRSQPAQEQDDEDREADAGSPAGPRGAPVPAPKQRSRLATRFQVTAHWLLVAAGRTLAILLLALAGIAQPSAFSGVYFLVFLATCTWWACHFPIGPLGSSTLCVMVGCFSAGHLICVYCYQTPFAQAVLPPAGIWARVFGLKALVAYSNCSGPNVLVLDTSHDWPVYVSPGVLLLLYYTVTSLLKLCTHQPLDQRKEAAGRDEEQEVELTHVDQWPQGRAGAAATKEEGAAQRVPPTTTGPDWETDSCTVHDLTGHSPVRQRPLHPRLSEPRDVSPLHGLGHLIMDQSYVCALIAMMVWSITYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPFILLYGLALCGLRYVWAMDLRPELPTTLGPVSLRQLGLEHTRYPCLDLGAMLLYNLTFWLLLRQFVKEKLLRKAKSAAGLLGVAVADPEPTRTRTLLRSLGELVTGLYAKYWIYVCAGMFVVVSFAGRLVVYKIVYMLLFLLCLTLFQVYYSLWRKLLKAFWWLVVAYTMLVLSAVYTFQFQDFPTYWRNLTGLTDEQLADLGLEQFSVSELFSSILVPGFFLLACILQLHYFHQPFMRLTDLGRAPPPGAHLPRWARRQDAVSGTPLLQEEEEVAREEGLSEAGPQQAVQVPEAAASKWGLVAERLLDLADRFSEVLTRVQVLVRRLLELHVLKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPnullASCLSTVWTCIIIVCKMLYQLKVVSPHEYSSNCTEPLLNSTNLQKTEIKQSLLYRGPVDPANWFGVRKGFPNLGYIQNHLLVLLLLVFEAIVYRRQEHHRRQHQPAPLPAQAVCADGTRQRLDQDLLSCFKYFVNFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAILTRRHRGAIARLWPNYCLFLALFFLYQYLLCLGIPPALCLDYPWRWSQAIPMNSALIKWLYLPDFFRTPNSTNLISDFLLLLCASQQWQVFAAERTEEWQHVAGTNTDRLEVPQGEPDPVPNFIYCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTSLLQKHTRARLLLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSSFCWVIQLFSLVCTVKGYYDPKEMLSRDRDCLLPVEEAGVLWDSVCFLFLLLQRRVFLSRYFLHVSAELQATALQASRGFALYNAANLKSIELHRKAEEKSLAQLKRQMERIRAKQEKHRQSRAGRSRPQEPQGPPQEPGPGSPRGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEETPPEDPRPSAQSAFQLAYQAWVTNAQTVLRRQREEQARQEQAGQPPAGGGPGAEAEPAEGPDQEAAGHSHVMQRVLSAVQFLWVLGQALVDGLTDWLRTFTRHHRAMSDVLRAERYLYTQALLRGQEVHRGLLDQLYARGVEAAPPGSSGARDAPGAAASGPGAQEPLSAATEATGGHLSTGCNARSSGEDATAEPGASLHSSRELPAGASTRTRTASELLLNRHLHIQELEEAQQFEARQGRALRLLQAAYQCVAAHSELLCYFVIILNHMVTASATSLVLPVLVFLWAMLSIPRPSKRFWMTAIVFTEVTVVTKYLFQFGFFPWNTHAVLRRYENKPYFPPRILGLEKTDGYVKYDLVQLMALFFHRSQLLCYGLWDHEGDQLSKDHDRGTGKKGVEEEPTSPGPQGEPGVAGALAQYHMQAEAGDGPPEPPVEPKPRDPKPISLRFRKRRRESTEPVEPVEPVEPTAVEGVAAAPGSERWSRPRERARALGLRLQSCCLALAQSVYRPLHCFFHDILHTQYRAATDVYALMFLADVVDFIIIIFGFWAFGKHSAATDITSSLSDDQVPEAFLVMLLIQFSTMVIDRALYLRKTVLGKLAFQVVLVLAIHLWMFLILPAVTERMFSQNAVAQLWYFVKCVYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSSWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKVVKYGMGGLIILFLVAIIWFPLLFMSLVRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTQQAYEELSRHFDPNPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYTNEKHTLDLAANSSERRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRRERVGSGAAGFLEWWVIELQDCQAECNLLPMVIFSDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Target ORF information:

RefSeq Version	XM_023244898.1
Organism	Felis catus(domestic cat)
Definition	Felis catus piezo type mechanosensitive ion channel component 1 (PIEZO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023244898.1

ORF Insert Sequence:

ATGTGGGCCT GTCCCTGCCC GAGCTTGGGG TCTCCTGGCC CGGCTCCCAG ACTCCGGTGG 
GATGTGGGTC CCCCAGGACG GGCGGAAGAA GGCCCTGTGC CAGCCTCGCC CCTGCCCTCT 
CTGCCCGCAG GTCACACTGG TCGCCTCCTC CGAGCCCTGC TGCTTTTCAG CCTCCTCTTC 
CTGGCAGCCC ACCTGGCCTT CCAGATATGC CTGCACACCG TGCCCCGCCT GGACCAGCTC 
CTGGGGCCAA GCTGCAGCCC CTGGCAGATC CTGTCCCAGC ACGTAGGGGT GACGAGGCTG 
GACCTGAAGG ACATCCCCAA TGCCGTCCGG CTGGTGGCCC CTGACCTGGG TGTCCTGGTG 
GTCTCAGCCG TGTGCCTCGG CCTCTGTGGG CGCCTGGCAC GGGAAGCCCG GCGGAGCCAG 
CCCGCCCAGG AGCAGGACGA TGAGGACAGG GAAGCGGACG CCGGCTCCCC GGCGGGGCCT 
CGCGGAGCCC CCGTGCCGGC CCCCAAGCAG AGGTCTCGGC TGGCCACCCG GTTCCAGGTC 
ACGGCCCACT GGCTGCTGGT GGCCGCCGGA CGGACGCTGG CCATCCTGCT GCTGGCGCTG 
GCAGGCATCG CCCAGCCCTC GGCCTTCTCC GGCGTCTACT TCCTGGTCTT CCTGGCCACC 
TGCACCTGGT GGGCCTGCCA CTTTCCCATC GGCCCCCTGG GCTCCAGCAC GCTCTGCGTC 
ATGGTGGGCT GCTTCAGCGC CGGCCACCTC ATCTGCGTCT ACTGCTACCA GACGCCCTTC 
GCCCAGGCCG TGCTGCCCCC TGCCGGCATC TGGGCCAGGG TGTTCGGCCT CAAGGCCTTG 
GTGGCCTACA GCAACTGCTC CGGTCCCAAC GTGCTGGTCC TCGATACCAG CCATGACTGG 
CCCGTGTACG TGAGCCCCGG CGTCCTGCTG CTCCTCTACT ACACCGTGAC CTCACTCCTC 
AAGCTGTGCA CACACCAGCC CCTGGACCAG AGGAAGGAGG CGGCCGGGCG CGACGAGGAG 
CAGGAGGTGG AACTGACTCA CGTGGATCAG TGGCCCCAGG GCCGGGCCGG GGCTGCGGCC 
ACCAAGGAGG AGGGCGCCGC CCAGCGCGTG CCGCCCACGA CCACGGGACC AGACTGGGAG 
ACCGACAGCT GCACCGTGCA CGACCTGACC GGCCACAGCC CCGTCCGGCA GCGCCCCCTG 
CACCCCAGGC TGTCGGAGCC TAGAGACGTG TCTCCCCTGC ACGGTCTGGG CCATCTCATC 
ATGGACCAGA GCTACGTGTG TGCCCTCATC GCCATGATGG TGTGGAGTAT CACCTACCAC 
AGCTGGCTGA CCTTCGTGCT GCTGCTCTGG GCCTGCCTCA TCTGGACGGT GCGCAGTCGT 
CACCAGCTGG CCATGCTCTG TTCCCCCTTC ATCCTGCTCT ACGGGCTGGC GCTGTGCGGG 
CTCCGCTACG TGTGGGCCAT GGACCTGCGT CCCGAGCTGC CCACCACCCT GGGCCCCGTC 
AGCCTGCGTC AGCTGGGGCT GGAGCACACC CGCTACCCTT GCCTGGACCT TGGTGCCATG 
CTGCTGTACA ACCTGACCTT CTGGCTCCTG CTGCGCCAGT TCGTGAAGGA GAAGCTGCTA 
AGGAAGGCCA AGAGCGCTGC CGGCCTGCTG GGGGTCGCCG TGGCCGACCC AGAGCCCACG 
CGGACACGGA CGCTGCTCCG GAGCCTGGGC GAGCTGGTCA CGGGCCTGTA CGCCAAGTAC 
TGGATCTACG TGTGCGCCGG CATGTTCGTC GTGGTCAGCT TTGCCGGCCG CCTCGTCGTC 
TACAAGATCG TGTACATGCT CCTCTTCCTG CTGTGCCTCA CGCTCTTCCA GGTCTACTAC 
AGCCTGTGGA GGAAGCTGCT CAAGGCGTTC TGGTGGCTCG TGGTAGCCTA CACCATGCTG 
GTGCTCAGCG CCGTCTACAC CTTCCAGTTC CAGGACTTCC CCACCTACTG GCGCAACCTG 
ACCGGCCTCA CGGACGAGCA GCTGGCGGAC CTGGGTCTGG AGCAGTTCAG CGTGTCCGAG 
CTCTTCTCCA GCATCCTGGT CCCCGGCTTC TTCCTGCTGG CCTGCATTCT GCAGCTGCAT 
TATTTCCACC AGCCCTTCAT GCGGCTCACC GACCTGGGGC GCGCGCCCCC GCCCGGCGCC 
CACCTCCCGC GCTGGGCTCG AAGGCAGGAC GCGGTCAGCG GGACCCCGCT GCTGCAGGAG 
GAGGAGGAGG TGGCCAGGGA GGAGGGACTG AGCGAGGCCG GCCCCCAGCA GGCCGTGCAG 
GTCCCCGAAG CTGCCGCCAG CAAGTGGGGC CTGGTGGCCG AGCGGCTGCT GGACCTGGCT 
GACCGCTTCT CCGAGGTCCT CACCCGCGTG CAGGTGTTGG TGCGACGCCT GCTGGAGCTG 
CACGTCCTCA AGCTGGTGGC CCTGTACACT GTGTGGGTGG CCCTGAAGGA GGTGTCGGTG 
ATGAACCTGC TTCTGGTCGT GCTGTGGGCC TTCGCCCTGC CCTACCCCCG CTTCCGGCCA 
NTGGCCTCCT GCCTGTCCAC CGTGTGGACC TGTATCATCA TCGTGTGCAA GATGCTGTAT 
CAGCTGAAGG TCGTCAGCCC GCACGAATAC TCCAGCAACT GCACCGAGCC CCTCCTCAAC 
AGCACCAACC TGCAGAAAAC GGAGATCAAG CAGTCCCTGC TGTACCGGGG ACCCGTCGAC 
CCTGCCAACT GGTTTGGGGT CCGGAAGGGC TTCCCGAACC TGGGCTACAT CCAGAACCAC 
CTGCTGGTCC TGCTGCTGCT GGTGTTCGAG GCCATTGTTT ACCGGCGCCA GGAGCACCAC 
CGTCGGCAGC ACCAGCCGGC CCCGCTGCCT GCCCAGGCCG TCTGTGCCGA CGGCACCCGC 
CAGCGGCTGG ACCAAGATCT TCTCAGCTGC TTCAAGTACT TCGTCAACTT CTTTTTCTAC 
AAATTCGGGC TGGAGATCTG CTTCCTGATG GCCGTGAACG TGATTGGGCA GCGCATGAAT 
TTCATGGTCA TCCTGCACGG CTGCTGGCTG GTGGCCATTC TTACCCGCCG GCACCGCGGA 
GCCATCGCCC GCCTCTGGCC CAACTACTGT CTGTTCCTGG CGTTGTTCTT CCTGTACCAG 
TACCTGCTCT GCCTGGGCAT CCCCCCGGCC CTGTGCCTCG ACTACCCGTG GCGGTGGAGC 
CAGGCCATCC CCATGAACTC CGCGCTCATC AAGTGGCTGT ACCTGCCCGA CTTCTTCAGA 
ACCCCCAACT CCACCAACCT CATCAGTGAC TTCCTCCTGC TGCTGTGCGC CTCCCAGCAG 
TGGCAGGTGT TCGCAGCCGA GCGCACGGAA GAGTGGCAGC ACGTGGCCGG CACCAACACT 
GACCGCCTGG AGGTGCCGCA GGGCGAGCCC GACCCCGTGC CCAACTTCAT TTACTGCAGG 
TCCTACCTCG ACATGCTGAA GGTGGCCGTC TTCCGCTACC TCTTCTGGCT GGTGCTGGTG 
GTGGTGTTCG TCACGGGAGC CACCCGCATC AGTATCTTCG GGCTGGGCTA CCTGCTGGCC 
TGCTTCTACC TGCTGCTCTT CGGCACCAGC CTGTTGCAGA AGCACACGCG TGCCCGCCTC 
CTGCTGTGGG ACTGCCTCAT TCTGTACAAC GTCACCGTCA TCATCTCCAA GAACATGCTT 
TCGCTCCTGT CCTGCGTGTT CGTGGAGCAA ATGCAGAGCA GCTTCTGCTG GGTCATCCAG 
CTCTTCAGCC TCGTGTGCAC CGTCAAAGGC TACTATGACC CCAAGGAGAT GCTGAGCCGG 
GACCGGGACT GCCTGCTGCC CGTGGAGGAG GCCGGCGTGC TCTGGGACAG CGTGTGCTTC 
CTGTTCCTGC TGCTCCAGCG CCGCGTGTTC CTCAGCCGCT ACTTCCTGCA CGTCAGTGCG 
GAGCTCCAGG CCACCGCCTT GCAGGCCTCC AGGGGCTTCG CGCTGTACAA CGCGGCCAAC 
CTCAAGAGCA TCGAGCTCCA TCGCAAGGCG GAGGAGAAGT CCCTGGCCCA GCTGAAGAGA 
CAGATGGAGC GCATCCGCGC CAAACAGGAG AAGCACAGGC AGAGCCGGGC CGGCCGCAGC 
CGCCCCCAGG AGCCCCAGGG CCCCCCCCAG GAGCCGGGGC CTGGCAGTCC ACGGGGCTCG 
TCCCCGCCAC GGAGGCAGTG GTGGCGGCCC TGGCTGGACC ACGCCACAGT TATCCACTCG 
GGGGACTACT TCCTGTTCGA GTCTGACAGT GAGGAGGAGG AGGAGACCCC ACCTGAGGAC 
CCCAGGCCTT CGGCACAGAG CGCTTTCCAG CTGGCGTACC AGGCATGGGT GACCAACGCG 
CAGACGGTGC TGAGGAGGCA GCGGGAGGAG CAGGCGAGGC AGGAGCAGGC AGGGCAGCCG 
CCCGCGGGAG GCGGCCCGGG CGCAGAGGCG GAGCCGGCGG AGGGCCCAGA CCAAGAGGCG 
GCTGGCCACA GCCACGTGAT GCAGCGGGTG CTGAGCGCCG TGCAGTTTCT GTGGGTGCTG 
GGACAGGCGC TGGTGGACGG GCTGACGGAC TGGCTGCGCA CCTTCACGCG GCACCACCGC 
GCCATGAGTG ACGTGTTGCG CGCCGAGCGC TACCTGTACA CGCAGGCGCT GCTCAGGGGC 
CAAGAGGTAC ACCGGGGCCT GCTGGACCAG CTGTATGCCA GGGGCGTGGA GGCCGCACCA 
CCAGGCTCCT CGGGGGCCCG AGACGCACCT GGCGCGGCGG CAAGTGGGCC GGGGGCACAG 
GAGCCACTGA GCGCCGCCAC GGAGGCCACC GGCGGCCACC TGAGCACGGG GTGCAACGCC 
CGCAGCAGCG GCGAGGACGC GACGGCCGAG CCTGGGGCTT CCCTGCACAG CTCCCGGGAG 
CTTCCCGCCG GTGCTTCCAC CCGGACCCGA ACCGCCAGCG AGCTGCTCCT CAACAGGCAC 
CTGCACATCC AGGAGCTGGA GGAGGCCCAG CAGTTCGAAG CGAGGCAGGG CCGGGCGCTG 
CGGCTGCTGC AGGCCGCCTA CCAGTGCGTG GCCGCCCACT CGGAGCTGCT CTGCTATTTC 
GTCATCATCC TCAACCACAT GGTCACCGCC TCGGCCACCT CCCTGGTGCT GCCCGTGCTC 
GTCTTCCTGT GGGCCATGCT GTCCATCCCG AGGCCCAGCA AGCGCTTCTG GATGACGGCC 
ATCGTCTTCA CCGAGGTGAC CGTGGTCACC AAGTACCTGT TCCAGTTTGG CTTCTTCCCC 
TGGAACACCC ACGCGGTGCT CCGGCGCTAC GAGAACAAGC CGTACTTCCC ACCACGGATC 
CTGGGCCTGG AGAAGACCGA CGGCTACGTC AAGTACGACC TAGTACAGCT CATGGCCTTG 
TTCTTCCACC GCTCCCAGCT GCTGTGCTAT GGCCTCTGGG ACCATGAAGG GGACCAACTC 
TCCAAGGACC ACGACCGCGG CACTGGGAAG AAGGGGGTTG AGGAGGAGCC GACGTCACCA 
GGACCCCAGG GAGAACCAGG GGTGGCTGGG GCCCTCGCCC AGTACCACAT GCAGGCGGAA 
GCAGGGGATG GGCCCCCAGA GCCTCCCGTG GAGCCCAAGC CCCGAGACCC AAAGCCGATC 
AGCCTGCGTT TCAGGAAGAG GAGAAGGGAG AGCACGGAGC CCGTGGAGCC CGTGGAGCCC 
GTGGAGCCCA CGGCTGTGGA AGGCGTGGCG GCGGCGCCGG GGAGCGAGAG GTGGAGTCGC 
CCCCGGGAAA GGGCGAGAGC CCTGGGGCTC CGACTGCAGA GCTGCTGCCT GGCCCTGGCC 
CAGAGCGTGT ACCGGCCCCT GCACTGCTTT TTCCACGACA TCCTGCACAC CCAGTACCGC 
GCGGCCACCG ACGTCTACGC CCTTATGTTC CTGGCCGACG TCGTTGACTT CATCATCATC 
ATCTTTGGCT TCTGGGCCTT TGGGAAACAC TCGGCAGCCA CAGACATCAC GTCCTCACTG 
TCAGACGACC AGGTGCCCGA GGCCTTCCTG GTCATGCTGC TCATCCAGTT CAGCACCATG 
GTCATCGACC GCGCCCTCTA CCTCCGCAAG ACCGTGCTGG GCAAGCTGGC CTTCCAGGTG 
GTCCTGGTGC TGGCCATCCA CCTCTGGATG TTCCTCATTC TGCCCGCGGT CACCGAGAGG 
ATGTTCAGCC AGAACGCAGT GGCCCAGCTG TGGTACTTCG TCAAGTGCGT CTACTTCGCC 
CTGTCCGCCT ACCAGATCCG CTGCGGCTAC CCCACCCGCA TCCTGGGCAA CTTCCTCACC 
AAGAAGTACA ACCACCTCAA CCTCTTCCTC TTCCAGGGTT TCCGGCTCGT GCCGTTCCTG 
GTGGAGCTGC GGGCCGTGAT GGACTGGGTG TGGACGGACA CCACGCTGTC CCTCTCCAGC 
TGGATGTGTG TGGAGGACAT CTACGCCAAC ATTTTCATCA TCAAGTGCAG CCGAGAGACC 
GAGAAGAAAT ACCCGCAGCC CAAGGGGCAG AAGAAGAAGA AGGTGGTCAA GTACGGCATG 
GGCGGGCTCA TCATCCTGTT CCTCGTGGCC ATCATCTGGT TCCCTCTGCT CTTTATGTCC 
CTGGTGCGCT CCGTGGTCGG CGTCGTCAAC CAGCCGATCG ACGTCACCGT CACCCTCAAG 
CTGGGTGGCT ACGAGCCCTT GTTCACCATG AGCGCGCAGC AGCCGTCCAT CGTGCCCTTC 
ACGCAACAGG CCTATGAGGA GCTGTCCAGA CACTTCGACC CGAACCCTCT GGCCATGCAG 
TTCATCAGCC AGTACAGCCC CGAGGACATC GTCACGGCGC AGATCGAGGG CAGCTCCGGG 
GCGCTGTGGC GCATCAGCCC GCCCAGCCGG GCCCAGATGA AGCGGGAGCT GTACAACGGC 
ACCGCCGACA TCACCCTGCG CTTCACCTGG AACTTCCAGA GGGACCTGGC CAAGGGCGGC 
ACCGTGGAAT ACACCAACGA GAAGCACACC CTGGACCTGG CTGCCAATAG CAGCGAGCGG 
AGGCAGCTGG CCAGCCTGCT GGAGGGCACC TCGGACCAGT CGGTGGTCAT CCCCAATCTC 
TTCCCCAAGT ACATCCGTGC TCCCAACGGG CCCGAGGCCA ACCCCGTGAA GCAGCTGCAG 
CCCAACGAGG AGGCCGACTA CCTCGGAGTG CGCATCCAGC TGCGGAGGGA GCGCGTGGGC 
TCGGGGGCCG CCGGCTTCCT GGAGTGGTGG GTCATCGAGC TGCAGGACTG CCAGGCCGAA 
TGCAACCTGC TCCCCATGGT CATCTTCAGC GACAAGGTCA GCCCGCCCAG CCTCGGCTTC 
CTGGCCGGCT ACGGGATCAT GGGGCTCTAC GTGTCCATCG TGCTGGTCAT CGGCAAGTTC 
GTGCGTGGCT TCTTCAGCGA GATCTCCCAC TCCATCATGT TTGAGGAGCT GCCCTGCGTG 
GACCGCATCC TCAAGCTCTG CCAGGACATC TTCCTGGTGC GGGAGACGCG CGAGCTGGAG 
CTGGAGGAGG AGCTCTACGC CAAGCTCATC TTCCTGTACC GCTCGCCCGA GACCATGATC 
AAGTGGACCC GCGAGAAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PIEZO1 cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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