LOC106585428 cDNA ORF clone, Salmo salar(Atlantic salmon)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106585428
Entrez Gene ID	106585428
Full Name	voltage-dependent N-type calcium channel subunit alpha-1B-like
Gene Type	protein-coding
Organism	Salmo salar(Atlantic salmon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014171608.1	XP_014027083.1	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X2
XM_014171611.1	XP_014027086.1	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X5
XM_014171607.1	XP_014027082.1	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X1
XM_014171610.1	XP_014027085.1	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X4
XM_014171613.1	XP_014027088.1	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X7
XM_014171609.1	XP_014027084.1	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X3
XM_014171614.1	XP_014027089.1	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X8
XM_014171612.1	XP_014027087.1	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X6

Pathways from BioSystems
Gene Ontology

The following LOC106585428 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106585428 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAt67164	XM_014171607.1 Latest version!	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAt67165	XM_014171608.1 Latest version!	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAt67166	XM_014171609.1 Latest version!	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAt67167	XM_014171610.1 Latest version!	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAt67168	XM_014171611.1 Latest version!	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAt67169	XM_014171612.1 Latest version!	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAt67170	XM_014171613.1 Latest version!	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAt67171	XM_014171614.1 Latest version!	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106585428 ( XM_014171607.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014027082.1 Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAt67164
Clone ID Related Accession (Same CDS sequence)	XM_014171607.1
Accession Version	XM_014171607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012340658.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTTTTGT AAAGCAAAGT 
CGAGGTGCAC TCTCTCGCAG CTCGTCCGTC TGCAGCGTTA ATTCACTGAG TTATGTCTGC 
TCCCCAGTCC ATCACTACTT GAGTAAGGAG CAGCAGAAGT CTCTGAAGAT GATGTCAGTG 
TGGGAGCAGC GCACCACTCA GCTGAGGAGA CACAACCTGC GGGCCAGCAG TGAGGCCCTG 
TACAGCGAGC TGGACCCTGA GGAGAGGCTA CGCATGTCCT CCGCCCTGCA CATCCGCCCT 
GACATGAAGA CCCACCTGGA CCGGCCCCTG GTCGTGAAGC CCCACGACGG CCCCGGGCCC 
ATGGGCCACC TCAAGCCCCA GACCCCTGAG GACACGGATA ACCCTGGGGA CCCGGCCGGC 
CCCCAGCCAC GTAGGCACCA CCGGGACAGG GAGAGACCCA TCGACGAGGC CAATGAGAAT 
GGAGATCCAG GCAAAGAGGG TAGGCACCAT GTCCATCACA GTCGCAGCAA GTACCCCAAT 
GGGACTCGGG GTAAGGAGGG GAAGAGTGAG CGGTCGCATA GCCGAGAGGG TGGCAGGAGG 
CACCACCACC AGACCTCAGT GGACGAGGGA GGTGGAGGGG GAACTGGGAC GGGAGAGAGA 
GTGCACCGCC ACCACCACTC CCACAGACAG GCCAGAGAGG GTAACAGCAC TGTCAACAGC 
GGACGCAAGG ATGAGCGCAG GTCACGCCAC AAAGATGGAC GCTCGGGAGA GCGATGCTCC 
AGGGGAGAGA ACGGGGCCAA TGGGGAGAGG AGGAGACACA AGACCCACAG TTACAGGGGC 
CAGTCCACAC TGGCGGGAGA GGAGCACAGT GAGAGGGAGA GGGGGAAGGG CCACAGGGAC 
AGATATGTTG AGTCTGAGGG CGACTCCATG GCCACTAGCA CCCAGCAGGA TCCTGGAGAT 
CAGAGGTGGG GCACAGAGAT AACTGAGGAC TCAGACAACC AGAGGAACGT CAGACGGACA 
GGATGGACGG CCGTCCACAT ACCCCCTGTC ACCATCACCT CCCTGCCTGG AGAGACCACC 
CTTATCCCCA TGAACAGTAT AGACTGTGAG ACATTGCCTA TGAACGAGGA GAAGAAGACC 
CTGGAGGTGT TGACTCGGAA CGGACCCAGA CCCATCCTCC CCTACAGCTC CATGTTTGTG 
TTTGGCCAGA CCAACATGGT GCGGCGTCTG TGTCATTACA TTGTGAGCCT GCGGTACTTT 
GAGATGTGTA TCCTGGTAGT GATATCTATG AGTAGCATTG CATTGGCTGC TGAGGACCCT 
GTCCAGGCCA ATGCTCCACG CAACAATGTG CTCAAGTATC TGGACTACGT CTTTACGGGA 
GTTTTCACAT TTGAGATGGT GATTAAGATG GTTGACCTGG GCCTTCTGCT CCATCCTGGT 
TCTTACTTCA GAGATCTCTG GAACATTCTG GACTTCATTG TGGTCAGTGG AGCACTGGTG 
GCATTTGCAT TTTCGAGCTT CATGGGACCG CCACAAGGTG TGCCCAGAGG GTCCAAAGGA 
AAGGACATCA GCACCATCAA GTCCCTCAGA GTTCTGCGAG TCTTACGGCC CCTCAAGACC 
ATCAAGCGCC TGCCAAAGCT GAAGGCTGTG TTTGACTGTG TGGTCAACTC CCTGAAGAAC 
GTCTTCAACA TCCTCATTGT CTACATGCTC TTCATGTTCA TCTTCGCTGT CATCGCTGTG 
CAGCTGTTCA AGGGAAAGTT CTTTTACTGC TCCGATGAGT CCAAGGGACT GGAAAAGGAC 
TGCAGAGGTC ATTTCCTGGA CTACGATAAG GATGAGGTGG CCGCCCAGCC TCGGGTGTGG 
AAGAAATATG AGTTCCACTA TGACAACGTG CTGTGGGCCT TCCTCACTCT CTTCACTGTG 
TCCACTGGAG AGGGCTGGCC CGATGTTCTG AAGCACTCTG TGGATGCTAC CTTTGAGGAC 
CAGGGTCCCA GTCCAGGCTA CCGTATAGAG ATCTCCATCT TCTACGTGGT CTACTTCGTG 
GTCTTTCCCT TCTTCTTCGT CAACATCTTT GTGGCCTTGA TCATCATTAC CTTCCAGGAG 
CAAGGGGACA AGGCCATGTC AGAGTGCAGC CTGGAGAAAA ACGAGAGGGC CTGCATTGAC 
TTTGCCATCA ACGCCAAGCC ACTGACGCGT TACATGCCAG AGAACGTGCA GTCCTTCCAG 
TACCGGATGT GGAAGTTTGT GGTGTCACCC CCGTTTGAGT ACGCCATTAT GATCATGATC 
GCCCTGAACA CCGTGGTGCT CATGATGAAG TTCCATGGAG CTCCAGACTT CTACGAGGCC 
ATGTTGAAGC ACTTCAACAT CGTCTTCACT GCCCTCTTCT CTCTGGAGTG CATTCTGAAG 
ATCATCGCCT TCGGACCACT GAACTACCTG AAGGATGCCT GGAACGTGTT TGACTTTGTG 
ACCGTGCTGG GAAGTATAAC TGACATCGTG GTCACAGAAA TCAATACTAC GGACCGGCTT 
TTGAACCTGA GTTTTCTGAG GCTGTTCAGA GCAGCTCGTC TCATCAAACT ACTGAGGCAA 
GGCTACACCA TCCGTATCCT GCTGTGGACC TTCGTCCAGT CCTTTAAGGC CCTGCCCTAT 
GTTTGCCTTC TGATAGCAAT GCTGTTCTTC ATCTATGCCA TCATCGGTAT GCAGGTGTTT 
GGAAACATTG AGCTCAACGA GGACACATCC ATCACTCACC ACAACAACTT CCGCACTTTT 
CTTCAGGCTC TCATGCTACT GTTCAGGAGT GCCACAGGGG AGGCGTGGCA TGAGATCATG 
CTGTCGTGTT TGAGTCACAG AGCCTGTGAT GAGAGGTCAG GCAACCTGGG GAAGGAGTGT 
GGCAGCGACT TTGCCTATTT CTACTTTGTG TCCTTCATCT TCCTCTGCTC TTTCCTGATG 
CTTAATCTGT TTGTGGCTGT GATCATGGAT AACTTTGAAT ACCTGACTCG AGACTCTTCT 
ATACTGGGGC CTCATCATCT GGATGAGTTC ATCCGTGTTT GGGCTGAGTA CGACCCTGCT 
GCCTGTGGCC GTATCAAGTA CCTCGATATG TATCAGATAC TCCTCCACAT GTCCCCACCC 
TTAGGTTTGG GAAAGAAATG TCCGCCAAGA GTCGCCTACA AGCGGCTAGT CAAGATGAAC 
ATGCCCATCG CTGATGACAA CACAGTCCAC TTCACCTCCA CACTCATGGC CCTGATCCGG 
ACGGCCCTGG AGATCAAACT GGCCTCAGGG ATGGTTGCCC AGCGTCTCTC TGATGCTGAA 
CTGAAGAAAG AGCTCTCCCA GGTTTGGCCA AGCCTCTCAC AGAAGACTAT GGATCTGTTG 
GTGACACCAC ACAAACCCAA TGAGCTTACT GTTGGGAAGG TGTATGCAGC CCTGATGATC 
TTTGACTACT ACAAGCAGAA CCGAGCCAAG AGGCTCAAGG AACAGCAACA GCAGCAGCAG 
CAACAGAATG CTACTGGCTC CCAGGGAAAG GTGGGGGCTC TGTTTAAACC AATACTGCCC 
CTGACTCACA AGCAGGAGAA AGTGAAGGAG AAGGCCATGA CCAACGTGGA GCCTCCCTGT 
GAGCTACTGC CTCAGCCCCA GTCTCAGGCC CTACCTAGAC CCAGCTCCAC CCTGCTCAAC 
AACGGAGGCA CACTGCCAGG CCATGACGGC ACCATGAAAG GATCATCCTC CTGGGTGTCA 
GAGAAGTCTA GAGAGGTCCA CAGACCCAGG ATTAAGACAC CTGTCCAAAG GGGCCAGTCT 
GAGGATGTCC CAAACCTAAC CATCCCCCAG GAGTCAGTGG AGATGAGGAA GATGGACACC 
AGTTGCAGCA CAGTGCCTGG TCCTGGGTCT GGACTGGAGA GCCAGGGAAG AGCTGTGTCC 
ATGCCCCGCC TCAATGCAGA GCTGCAGAGG AGCAAGTCGC GCCACTCCCC GGGGACCCTG 
CTGGCACCTG TTCCTGACTG CAGCCCAATG AAACGCTCTG CGTCCACCCA CGCCCCCCAG 
CGCCCCCAGG AGGTGAACCT GCATGAACAC ACCCTGGAGA GGCCCAGCAC CGAAGAAAGG 
CGTCACCATC ACCGCCACCA CCACCGCTGC CACCACCACC GAGAGAGGGA CAAGAAGCAG 
CGGTCACTGG ACAGGACGCC AAGCACCATT GGTGCACCAG GTGACCCGGC TGCAGAGCCC 
TCGTCTAGAG ACAGGGTGCA GGACCGCGGC CGCTCCCATG AGAGGAAGAA CCACTCGACG 
GCGGGGGAGA AGCAGCGCTA CTACTCTTGC GATCGCTACA GCAGCAGGGA ACACTGCCAC 
ACCACCAAAT CTGCCACCAC TAGCTGTGCC ACCTCCCCCA GCGAGGTGCA GGAACCCAGC 
ATCAACAAGC AGGGTAGTGG TTCGGTTAAA GGCAGCCCAG TGATGTTGAC CTCAGGGGCT 
ATCACACCTA GCCGTGGACG CAGGCAGCTC CCACAGACCC CCCTGACCCC CCGCCCCGGG 
GTGACGTTCA AGACTGCAAA CTCCTCCCCT GTGCACTTTG TGTCTGCCCA GTGTGGCAGC 
GGCCTGCCCA CCCTGAGTCC TGGCCGGCTG AGCCGTGGGC TGTCTGAGCA CAACGCCCTG 
CTGCATAGTG GCACCTCTCA CATCCCCTCC CCCATCACCC GCATCAGCTC TGAGCCCTTC 
CTGGGCTACA GTCACAGTGA CCCCCAAGGC CAGGGTGGGC CGTACTGCAA CCTCCGGGAC 
GAGCTGCTGA GCCCTTATGG TGCCAGAGCA CACTCCCCTC GGACTGCACG TCCTCAAATG 
AGGCCGGGGG CCCTCCACCT GCCTCCGCCA CCCCAGCAGA GACGTGCGGT GCCCAACGGA 
TACCACTTCA CCTTTGGTGG TAATGGCAAC GCCAGCCTGG GCTCTGGCTC TGGTGCCAGG 
CCACCCAGGT ACTATCACGA GGCAGAGGAG GATGAATGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014027082.1
CDS	635..8059
Translation	MARFEEELSNRRGGVCQAGPPRVAGRPAGHPSGPRVYKQSMAQRARTMAIYNPIPVKQSCLTVNRSLFIFSEDCIIRKYAKKITEWPPFEYLILATIIANCIVLALEQHLPASDKTPMSERLDDTEPYFIAIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGMLATVGADFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGMLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQAAEFPCGMEAPARTCENGTVCQEYWIGPNYGITNFDNILFAILTVFQCITMEGWVDILYNANDASGNTWNWIYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKHAEEKSPLDGAWYKRKHNNPVLKRAKKSKNDLINAEEGEDHFTDVSSVGSPFTRATPPGSPFTRAAPPGSPFTRAAPPGSPFTRATPPGSPFTRATPPGSPFTRATPPGSPFTRASLKSSKNDSASYFRRKEKRLRFTIRRLVKAQSFYWTVLCLVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLTEMSMKMYGLGPRNYFHSSFNCFDFGVIIGSIFEVIWAVVKPGASYGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGMFNFEEETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESHGGVRWGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEVAITKKMALQMVKEVKEVSPMSAANISITALHSMLYTGQHGAERAMPASFVKQSRGALSRSSSVCSVNSLSYVCSPVHHYLSKEQQKSLKMMSVWEQRTTQLRRHNLRASSEALYSELDPEERLRMSSALHIRPDMKTHLDRPLVVKPHDGPGPMGHLKPQTPEDTDNPGDPAGPQPRRHHRDRERPIDEANENGDPGKEGRHHVHHSRSKYPNGTRGKEGKSERSHSREGGRRHHHQTSVDEGGGGGTGTGERVHRHHHSHRQAREGNSTVNSGRKDERRSRHKDGRSGERCSRGENGANGERRRHKTHSYRGQSTLAGEEHSERERGKGHRDRYVESEGDSMATSTQQDPGDQRWGTEITEDSDNQRNVRRTGWTAVHIPPVTITSLPGETTLIPMNSIDCETLPMNEEKKTLEVLTRNGPRPILPYSSMFVFGQTNMVRRLCHYIVSLRYFEMCILVVISMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGPPQGVPRGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVIAVQLFKGKFFYCSDESKGLEKDCRGHFLDYDKDEVAAQPRVWKKYEFHYDNVLWAFLTLFTVSTGEGWPDVLKHSVDATFEDQGPSPGYRIEISIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPENVQSFQYRMWKFVVSPPFEYAIMIMIALNTVVLMMKFHGAPDFYEAMLKHFNIVFTALFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDIVVTEINTTDRLLNLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIELNEDTSITHHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSHRACDERSGNLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYQILLHMSPPLGLGKKCPPRVAYKRLVKMNMPIADDNTVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSQVWPSLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLKEQQQQQQQQNATGSQGKVGALFKPILPLTHKQEKVKEKAMTNVEPPCELLPQPQSQALPRPSSTLLNNGGTLPGHDGTMKGSSSWVSEKSREVHRPRIKTPVQRGQSEDVPNLTIPQESVEMRKMDTSCSTVPGPGSGLESQGRAVSMPRLNAELQRSKSRHSPGTLLAPVPDCSPMKRSASTHAPQRPQEVNLHEHTLERPSTEERRHHHRHHHRCHHHRERDKKQRSLDRTPSTIGAPGDPAAEPSSRDRVQDRGRSHERKNHSTAGEKQRYYSCDRYSSREHCHTTKSATTSCATSPSEVQEPSINKQGSGSVKGSPVMLTSGAITPSRGRRQLPQTPLTPRPGVTFKTANSSPVHFVSAQCGSGLPTLSPGRLSRGLSEHNALLHSGTSHIPSPITRISSEPFLGYSHSDPQGQGGPYCNLRDELLSPYGARAHSPRTARPQMRPGALHLPPPPQQRRAVPNGYHFTFGGNGNASLGSGSGARPPRYYHEAEEDEWC

Target ORF information:

RefSeq Version	XM_014171607.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014171607.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTTTTGT AAAGCAAAGT 
CGAGGTGCAC TCTCTCGCAG CTCGTCCGTC TGCAGCGTTA ATTCACTGAG TTATGTCTGC 
TCCCCAGTCC ATCACTACTT GAGTAAGGAG CAGCAGAAGT CTCTGAAGAT GATGTCAGTG 
TGGGAGCAGC GCACCACTCA GCTGAGGAGA CACAACCTGC GGGCCAGCAG TGAGGCCCTG 
TACAGCGAGC TGGACCCTGA GGAGAGGCTA CGCATGTCCT CCGCCCTGCA CATCCGCCCT 
GACATGAAGA CCCACCTGGA CCGGCCCCTG GTCGTGAAGC CCCACGACGG CCCCGGGCCC 
ATGGGCCACC TCAAGCCCCA GACCCCTGAG GACACGGATA ACCCTGGGGA CCCGGCCGGC 
CCCCAGCCAC GTAGGCACCA CCGGGACAGG GAGAGACCCA TCGACGAGGC CAATGAGAAT 
GGAGATCCAG GCAAAGAGGG TAGGCACCAT GTCCATCACA GTCGCAGCAA GTACCCCAAT 
GGGACTCGGG GTAAGGAGGG GAAGAGTGAG CGGTCGCATA GCCGAGAGGG TGGCAGGAGG 
CACCACCACC AGACCTCAGT GGACGAGGGA GGTGGAGGGG GAACTGGGAC GGGAGAGAGA 
GTGCACCGCC ACCACCACTC CCACAGACAG GCCAGAGAGG GTAACAGCAC TGTCAACAGC 
GGACGCAAGG ATGAGCGCAG GTCACGCCAC AAAGATGGAC GCTCGGGAGA GCGATGCTCC 
AGGGGAGAGA ACGGGGCCAA TGGGGAGAGG AGGAGACACA AGACCCACAG TTACAGGGGC 
CAGTCCACAC TGGCGGGAGA GGAGCACAGT GAGAGGGAGA GGGGGAAGGG CCACAGGGAC 
AGATATGTTG AGTCTGAGGG CGACTCCATG GCCACTAGCA CCCAGCAGGA TCCTGGAGAT 
CAGAGGTGGG GCACAGAGAT AACTGAGGAC TCAGACAACC AGAGGAACGT CAGACGGACA 
GGATGGACGG CCGTCCACAT ACCCCCTGTC ACCATCACCT CCCTGCCTGG AGAGACCACC 
CTTATCCCCA TGAACAGTAT AGACTGTGAG ACATTGCCTA TGAACGAGGA GAAGAAGACC 
CTGGAGGTGT TGACTCGGAA CGGACCCAGA CCCATCCTCC CCTACAGCTC CATGTTTGTG 
TTTGGCCAGA CCAACATGGT GCGGCGTCTG TGTCATTACA TTGTGAGCCT GCGGTACTTT 
GAGATGTGTA TCCTGGTAGT GATATCTATG AGTAGCATTG CATTGGCTGC TGAGGACCCT 
GTCCAGGCCA ATGCTCCACG CAACAATGTG CTCAAGTATC TGGACTACGT CTTTACGGGA 
GTTTTCACAT TTGAGATGGT GATTAAGATG GTTGACCTGG GCCTTCTGCT CCATCCTGGT 
TCTTACTTCA GAGATCTCTG GAACATTCTG GACTTCATTG TGGTCAGTGG AGCACTGGTG 
GCATTTGCAT TTTCGAGCTT CATGGGACCG CCACAAGGTG TGCCCAGAGG GTCCAAAGGA 
AAGGACATCA GCACCATCAA GTCCCTCAGA GTTCTGCGAG TCTTACGGCC CCTCAAGACC 
ATCAAGCGCC TGCCAAAGCT GAAGGCTGTG TTTGACTGTG TGGTCAACTC CCTGAAGAAC 
GTCTTCAACA TCCTCATTGT CTACATGCTC TTCATGTTCA TCTTCGCTGT CATCGCTGTG 
CAGCTGTTCA AGGGAAAGTT CTTTTACTGC TCCGATGAGT CCAAGGGACT GGAAAAGGAC 
TGCAGAGGTC ATTTCCTGGA CTACGATAAG GATGAGGTGG CCGCCCAGCC TCGGGTGTGG 
AAGAAATATG AGTTCCACTA TGACAACGTG CTGTGGGCCT TCCTCACTCT CTTCACTGTG 
TCCACTGGAG AGGGCTGGCC CGATGTTCTG AAGCACTCTG TGGATGCTAC CTTTGAGGAC 
CAGGGTCCCA GTCCAGGCTA CCGTATAGAG ATCTCCATCT TCTACGTGGT CTACTTCGTG 
GTCTTTCCCT TCTTCTTCGT CAACATCTTT GTGGCCTTGA TCATCATTAC CTTCCAGGAG 
CAAGGGGACA AGGCCATGTC AGAGTGCAGC CTGGAGAAAA ACGAGAGGGC CTGCATTGAC 
TTTGCCATCA ACGCCAAGCC ACTGACGCGT TACATGCCAG AGAACGTGCA GTCCTTCCAG 
TACCGGATGT GGAAGTTTGT GGTGTCACCC CCGTTTGAGT ACGCCATTAT GATCATGATC 
GCCCTGAACA CCGTGGTGCT CATGATGAAG TTCCATGGAG CTCCAGACTT CTACGAGGCC 
ATGTTGAAGC ACTTCAACAT CGTCTTCACT GCCCTCTTCT CTCTGGAGTG CATTCTGAAG 
ATCATCGCCT TCGGACCACT GAACTACCTG AAGGATGCCT GGAACGTGTT TGACTTTGTG 
ACCGTGCTGG GAAGTATAAC TGACATCGTG GTCACAGAAA TCAATACTAC GGACCGGCTT 
TTGAACCTGA GTTTTCTGAG GCTGTTCAGA GCAGCTCGTC TCATCAAACT ACTGAGGCAA 
GGCTACACCA TCCGTATCCT GCTGTGGACC TTCGTCCAGT CCTTTAAGGC CCTGCCCTAT 
GTTTGCCTTC TGATAGCAAT GCTGTTCTTC ATCTATGCCA TCATCGGTAT GCAGGTGTTT 
GGAAACATTG AGCTCAACGA GGACACATCC ATCACTCACC ACAACAACTT CCGCACTTTT 
CTTCAGGCTC TCATGCTACT GTTCAGGAGT GCCACAGGGG AGGCGTGGCA TGAGATCATG 
CTGTCGTGTT TGAGTCACAG AGCCTGTGAT GAGAGGTCAG GCAACCTGGG GAAGGAGTGT 
GGCAGCGACT TTGCCTATTT CTACTTTGTG TCCTTCATCT TCCTCTGCTC TTTCCTGATG 
CTTAATCTGT TTGTGGCTGT GATCATGGAT AACTTTGAAT ACCTGACTCG AGACTCTTCT 
ATACTGGGGC CTCATCATCT GGATGAGTTC ATCCGTGTTT GGGCTGAGTA CGACCCTGCT 
GCCTGTGGCC GTATCAAGTA CCTCGATATG TATCAGATAC TCCTCCACAT GTCCCCACCC 
TTAGGTTTGG GAAAGAAATG TCCGCCAAGA GTCGCCTACA AGCGGCTAGT CAAGATGAAC 
ATGCCCATCG CTGATGACAA CACAGTCCAC TTCACCTCCA CACTCATGGC CCTGATCCGG 
ACGGCCCTGG AGATCAAACT GGCCTCAGGG ATGGTTGCCC AGCGTCTCTC TGATGCTGAA 
CTGAAGAAAG AGCTCTCCCA GGTTTGGCCA AGCCTCTCAC AGAAGACTAT GGATCTGTTG 
GTGACACCAC ACAAACCCAA TGAGCTTACT GTTGGGAAGG TGTATGCAGC CCTGATGATC 
TTTGACTACT ACAAGCAGAA CCGAGCCAAG AGGCTCAAGG AACAGCAACA GCAGCAGCAG 
CAACAGAATG CTACTGGCTC CCAGGGAAAG GTGGGGGCTC TGTTTAAACC AATACTGCCC 
CTGACTCACA AGCAGGAGAA AGTGAAGGAG AAGGCCATGA CCAACGTGGA GCCTCCCTGT 
GAGCTACTGC CTCAGCCCCA GTCTCAGGCC CTACCTAGAC CCAGCTCCAC CCTGCTCAAC 
AACGGAGGCA CACTGCCAGG CCATGACGGC ACCATGAAAG GATCATCCTC CTGGGTGTCA 
GAGAAGTCTA GAGAGGTCCA CAGACCCAGG ATTAAGACAC CTGTCCAAAG GGGCCAGTCT 
GAGGATGTCC CAAACCTAAC CATCCCCCAG GAGTCAGTGG AGATGAGGAA GATGGACACC 
AGTTGCAGCA CAGTGCCTGG TCCTGGGTCT GGACTGGAGA GCCAGGGAAG AGCTGTGTCC 
ATGCCCCGCC TCAATGCAGA GCTGCAGAGG AGCAAGTCGC GCCACTCCCC GGGGACCCTG 
CTGGCACCTG TTCCTGACTG CAGCCCAATG AAACGCTCTG CGTCCACCCA CGCCCCCCAG 
CGCCCCCAGG AGGTGAACCT GCATGAACAC ACCCTGGAGA GGCCCAGCAC CGAAGAAAGG 
CGTCACCATC ACCGCCACCA CCACCGCTGC CACCACCACC GAGAGAGGGA CAAGAAGCAG 
CGGTCACTGG ACAGGACGCC AAGCACCATT GGTGCACCAG GTGACCCGGC TGCAGAGCCC 
TCGTCTAGAG ACAGGGTGCA GGACCGCGGC CGCTCCCATG AGAGGAAGAA CCACTCGACG 
GCGGGGGAGA AGCAGCGCTA CTACTCTTGC GATCGCTACA GCAGCAGGGA ACACTGCCAC 
ACCACCAAAT CTGCCACCAC TAGCTGTGCC ACCTCCCCCA GCGAGGTGCA GGAACCCAGC 
ATCAACAAGC AGGGTAGTGG TTCGGTTAAA GGCAGCCCAG TGATGTTGAC CTCAGGGGCT 
ATCACACCTA GCCGTGGACG CAGGCAGCTC CCACAGACCC CCCTGACCCC CCGCCCCGGG 
GTGACGTTCA AGACTGCAAA CTCCTCCCCT GTGCACTTTG TGTCTGCCCA GTGTGGCAGC 
GGCCTGCCCA CCCTGAGTCC TGGCCGGCTG AGCCGTGGGC TGTCTGAGCA CAACGCCCTG 
CTGCATAGTG GCACCTCTCA CATCCCCTCC CCCATCACCC GCATCAGCTC TGAGCCCTTC 
CTGGGCTACA GTCACAGTGA CCCCCAAGGC CAGGGTGGGC CGTACTGCAA CCTCCGGGAC 
GAGCTGCTGA GCCCTTATGG TGCCAGAGCA CACTCCCCTC GGACTGCACG TCCTCAAATG 
AGGCCGGGGG CCCTCCACCT GCCTCCGCCA CCCCAGCAGA GACGTGCGGT GCCCAACGGA 
TACCACTTCA CCTTTGGTGG TAATGGCAAC GCCAGCCTGG GCTCTGGCTC TGGTGCCAGG 
CCACCCAGGT ACTATCACGA GGCAGAGGAG GATGAATGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106585428 ( XM_014171608.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014027083.1 Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X2, mRNA.

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CloneID	OAt67165
Clone ID Related Accession (Same CDS sequence)	XM_014171608.1
Accession Version	XM_014171608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012340658.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTTTTGT AAAGCAAAGT 
CGAGGTGCAC TCTCTCGCAG CTCGTCCGTC TGCAGCGTTA ATTCACTGAG TTATGTCTGC 
TCCCCAGTCC ATCACTACTT GAGTAAGGAG CAGCAGAAGT CTCTGAAGAT GATGTCAGTG 
TGGGAGCAGC GCACCACTCA GCTGAGGAGA CACAACCTGC GGGCCAGCAG TGAGGCCCTG 
TACAGCGAGC TGGACCCTGA GGAGAGGCTA CGCATGTCCT CCGCCCTGCA CATCCGCCCT 
GACATGAAGA CCCACCTGGA CCGGCCCCTG GTCGTGAAGC CCCACGACGG CCCCGGGCCC 
ATGGGCCACC TCAAGCCCCA GACCCCTGAG GACACGGATA ACCCTGGGGA CCCGGCCGGC 
CCCCAGCCAC GTAGGCACCA CCGGGACAGG GAGAGACCCA TCGACGAGGC CAATGAGAAT 
GGAGATCCAG GCAAAGAGGG TAGGCACCAT GTCCATCACA GTCGCAGCAA GTACCCCAAT 
GGGACTCGGG GTAAGGAGGG GAAGAGTGAG CGGTCGCATA GCCGAGAGGG TGGCAGGAGG 
CACCACCACC AGACCTCAGT GGACGAGGGA GGTGGAGGGG GAACTGGGAC GGGAGAGAGA 
GTGCACCGCC ACCACCACTC CCACAGACAG GCCAGAGAGG GTAACAGCAC TGTCAACAGC 
GGACGCAAGG ATGAGCGCAG GTCACGCCAC AAAGATGGAC GCTCGGGAGA GCGATGCTCC 
AGGGGAGAGA ACGGGGCCAA TGGGGAGAGG AGGAGACACA AGACCCACAG TTACAGGGGC 
CAGTCCACAC TGGCGGGAGA GGAGCACAGT GAGAGGGAGA GGGGGAAGGG CCACAGGGAC 
AGATATGTTG AGTCTGAGGG CGACTCCATG GCCACTAGCA CCCAGCAGGA TCCTGGAGAT 
CAGAGGTGGG GCACAGAGAT AACTGAGGAC TCAGACAACC AGAGGAACGT CAGACGGACA 
GGATGGACGG CCGTCCACAT ACCCCCTGTC ACCATCACCT CCCTGCCTGG AGAGACCACC 
CTTATCCCCA TGAACAGTAT AGACTGTGAG ACATTGCCTA TGAACGAGGA GAAGAAGACC 
CTGGAGGTGT TGACTCGGAA CGGACCCAGA CCCATCCTCC CCTACAGCTC CATGTTTGTG 
TTTGGCCAGA CCAACATGGT GCGGCGTCTG TGTCATTACA TTGTGAGCCT GCGGTACTTT 
GAGATGTGTA TCCTGGTAGT GATATCTATG AGTAGCATTG CATTGGCTGC TGAGGACCCT 
GTCCAGGCCA ATGCTCCACG CAACAATGTG CTCAAGTATC TGGACTACGT CTTTACGGGA 
GTTTTCACAT TTGAGATGGT GATTAAGATG GTTGACCTGG GCCTTCTGCT CCATCCTGGT 
TCTTACTTCA GAGATCTCTG GAACATTCTG GACTTCATTG TGGTCAGTGG AGCACTGGTG 
GCATTTGCAT TTTCGAGCTT CATGGGACCG CCACAAGGTG TGCCCAGAGG GTCCAAAGGA 
AAGGACATCA GCACCATCAA GTCCCTCAGA GTTCTGCGAG TCTTACGGCC CCTCAAGACC 
ATCAAGCGCC TGCCAAAGCT GAAGGCTGTG TTTGACTGTG TGGTCAACTC CCTGAAGAAC 
GTCTTCAACA TCCTCATTGT CTACATGCTC TTCATGTTCA TCTTCGCTGT CATCGCTGTG 
CAGCTGTTCA AGGGAAAGTT CTTTTACTGC TCCGATGAGT CCAAGGGACT GGAAAAGGAC 
TGCAGAGGTC ATTTCCTGGA CTACGATAAG GATGAGGTGG CCGCCCAGCC TCGGGTGTGG 
AAGAAATATG AGTTCCACTA TGACAACGTG CTGTGGGCCT TCCTCACTCT CTTCACTGTG 
TCCACTGGAG AGGGCTGGCC CGATGTTCTG AAGCACTCTG TGGATGCTAC CTTTGAGGAC 
CAGGGTCCCA GTCCAGGCTA CCGTATAGAG ATCTCCATCT TCTACGTGGT CTACTTCGTG 
GTCTTTCCCT TCTTCTTCGT CAACATCTTT GTGGCCTTGA TCATCATTAC CTTCCAGGAG 
CAAGGGGACA AGGCCATGTC AGAGTGCAGC CTGGAGAAAA ACGAGAGGGC CTGCATTGAC 
TTTGCCATCA ACGCCAAGCC ACTGACGCGT TACATGCCAG AGAACGTGCA GTCCTTCCAG 
TACCGGATGT GGAAGTTTGT GGTGTCACCC CCGTTTGAGT ACGCCATTAT GATCATGATC 
GCCCTGAACA CCGTGGTGCT CATGATGAAG TTCCATGGAG CTCCAGACTT CTACGAGGCC 
ATGTTGAAGC ACTTCAACAT CGTCTTCACT GCCCTCTTCT CTCTGGAGTG CATTCTGAAG 
ATCATCGCCT TCGGACCACT GAACTACCTG AAGGATGCCT GGAACGTGTT TGACTTTGTG 
ACCGTGCTGG GAAGTATAAC TGACATCGTG GTCACAGAAA TCAATACTAC GGACCGGCTT 
TTGAACCTGA GTTTTCTGAG GCTGTTCAGA GCAGCTCGTC TCATCAAACT ACTGAGGCAA 
GGCTACACCA TCCGTATCCT GCTGTGGACC TTCGTCCAGT CCTTTAAGGC CCTGCCCTAT 
GTTTGCCTTC TGATAGCAAT GCTGTTCTTC ATCTATGCCA TCATCGGTAT GCAGGTGTTT 
GGAAACATTG AGCTCAACGA GGACACATCC ATCACTCACC ACAACAACTT CCGCACTTTT 
CTTCAGGCTC TCATGCTACT GTTCAGGAGT GCCACAGGGG AGGCGTGGCA TGAGATCATG 
CTGTCGTGTT TGAGTCACAG AGCCTGTGAT GAGAGGTCAG GCAACCTGGG GAAGGAGTGT 
GGCAGCGACT TTGCCTATTT CTACTTTGTG TCCTTCATCT TCCTCTGCTC TTTCCTGATG 
CTTAATCTGT TTGTGGCTGT GATCATGGAT AACTTTGAAT ACCTGACTCG AGACTCTTCT 
ATACTGGGGC CTCATCATCT GGATGAGTTC ATCCGTGTTT GGGCTGAGTA CGACCCTGCT 
GCCTGCGGAC GGATTCGCTA TAACGATATG TACAACTTGT TACGAATTAT CTCACCCCCC 
CTTGGCTTAG GGAAGAACTG CCCTAATAGG GTAGCATACA AGCGGCTAGT CAAGATGAAC 
ATGCCCATCG CTGATGACAA CACAGTCCAC TTCACCTCCA CACTCATGGC CCTGATCCGG 
ACGGCCCTGG AGATCAAACT GGCCTCAGGG ATGGTTGCCC AGCGTCTCTC TGATGCTGAA 
CTGAAGAAAG AGCTCTCCCA GGTTTGGCCA AGCCTCTCAC AGAAGACTAT GGATCTGTTG 
GTGACACCAC ACAAACCCAA TGAGCTTACT GTTGGGAAGG TGTATGCAGC CCTGATGATC 
TTTGACTACT ACAAGCAGAA CCGAGCCAAG AGGCTCAAGG AACAGCAACA GCAGCAGCAG 
CAACAGAATG CTACTGGCTC CCAGGGAAAG GTGGGGGCTC TGTTTAAACC AATACTGCCC 
CTGACTCACA AGCAGGAGAA AGTGAAGGAG AAGGCCATGA CCAACGTGGA GCCTCCCTGT 
GAGCTACTGC CTCAGCCCCA GTCTCAGGCC CTACCTAGAC CCAGCTCCAC CCTGCTCAAC 
AACGGAGGCA CACTGCCAGG CCATGACGGC ACCATGAAAG GATCATCCTC CTGGGTGTCA 
GAGAAGTCTA GAGAGGTCCA CAGACCCAGG ATTAAGACAC CTGTCCAAAG GGGCCAGTCT 
GAGGATGTCC CAAACCTAAC CATCCCCCAG GAGTCAGTGG AGATGAGGAA GATGGACACC 
AGTTGCAGCA CAGTGCCTGG TCCTGGGTCT GGACTGGAGA GCCAGGGAAG AGCTGTGTCC 
ATGCCCCGCC TCAATGCAGA GCTGCAGAGG AGCAAGTCGC GCCACTCCCC GGGGACCCTG 
CTGGCACCTG TTCCTGACTG CAGCCCAATG AAACGCTCTG CGTCCACCCA CGCCCCCCAG 
CGCCCCCAGG AGGTGAACCT GCATGAACAC ACCCTGGAGA GGCCCAGCAC CGAAGAAAGG 
CGTCACCATC ACCGCCACCA CCACCGCTGC CACCACCACC GAGAGAGGGA CAAGAAGCAG 
CGGTCACTGG ACAGGACGCC AAGCACCATT GGTGCACCAG GTGACCCGGC TGCAGAGCCC 
TCGTCTAGAG ACAGGGTGCA GGACCGCGGC CGCTCCCATG AGAGGAAGAA CCACTCGACG 
GCGGGGGAGA AGCAGCGCTA CTACTCTTGC GATCGCTACA GCAGCAGGGA ACACTGCCAC 
ACCACCAAAT CTGCCACCAC TAGCTGTGCC ACCTCCCCCA GCGAGGTGCA GGAACCCAGC 
ATCAACAAGC AGGGTAGTGG TTCGGTTAAA GGCAGCCCAG TGATGTTGAC CTCAGGGGCT 
ATCACACCTA GCCGTGGACG CAGGCAGCTC CCACAGACCC CCCTGACCCC CCGCCCCGGG 
GTGACGTTCA AGACTGCAAA CTCCTCCCCT GTGCACTTTG TGTCTGCCCA GTGTGGCAGC 
GGCCTGCCCA CCCTGAGTCC TGGCCGGCTG AGCCGTGGGC TGTCTGAGCA CAACGCCCTG 
CTGCATAGTG GCACCTCTCA CATCCCCTCC CCCATCACCC GCATCAGCTC TGAGCCCTTC 
CTGGGCTACA GTCACAGTGA CCCCCAAGGC CAGGGTGGGC CGTACTGCAA CCTCCGGGAC 
GAGCTGCTGA GCCCTTATGG TGCCAGAGCA CACTCCCCTC GGACTGCACG TCCTCAAATG 
AGGCCGGGGG CCCTCCACCT GCCTCCGCCA CCCCAGCAGA GACGTGCGGT GCCCAACGGA 
TACCACTTCA CCTTTGGTGG TAATGGCAAC GCCAGCCTGG GCTCTGGCTC TGGTGCCAGG 
CCACCCAGGT ACTATCACGA GGCAGAGGAG GATGAATGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014027083.1
CDS	635..8059
Translation	MARFEEELSNRRGGVCQAGPPRVAGRPAGHPSGPRVYKQSMAQRARTMAIYNPIPVKQSCLTVNRSLFIFSEDCIIRKYAKKITEWPPFEYLILATIIANCIVLALEQHLPASDKTPMSERLDDTEPYFIAIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGMLATVGADFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGMLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQAAEFPCGMEAPARTCENGTVCQEYWIGPNYGITNFDNILFAILTVFQCITMEGWVDILYNANDASGNTWNWIYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKHAEEKSPLDGAWYKRKHNNPVLKRAKKSKNDLINAEEGEDHFTDVSSVGSPFTRATPPGSPFTRAAPPGSPFTRAAPPGSPFTRATPPGSPFTRATPPGSPFTRATPPGSPFTRASLKSSKNDSASYFRRKEKRLRFTIRRLVKAQSFYWTVLCLVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLTEMSMKMYGLGPRNYFHSSFNCFDFGVIIGSIFEVIWAVVKPGASYGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGMFNFEEETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESHGGVRWGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEVAITKKMALQMVKEVKEVSPMSAANISITALHSMLYTGQHGAERAMPASFVKQSRGALSRSSSVCSVNSLSYVCSPVHHYLSKEQQKSLKMMSVWEQRTTQLRRHNLRASSEALYSELDPEERLRMSSALHIRPDMKTHLDRPLVVKPHDGPGPMGHLKPQTPEDTDNPGDPAGPQPRRHHRDRERPIDEANENGDPGKEGRHHVHHSRSKYPNGTRGKEGKSERSHSREGGRRHHHQTSVDEGGGGGTGTGERVHRHHHSHRQAREGNSTVNSGRKDERRSRHKDGRSGERCSRGENGANGERRRHKTHSYRGQSTLAGEEHSERERGKGHRDRYVESEGDSMATSTQQDPGDQRWGTEITEDSDNQRNVRRTGWTAVHIPPVTITSLPGETTLIPMNSIDCETLPMNEEKKTLEVLTRNGPRPILPYSSMFVFGQTNMVRRLCHYIVSLRYFEMCILVVISMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGPPQGVPRGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVIAVQLFKGKFFYCSDESKGLEKDCRGHFLDYDKDEVAAQPRVWKKYEFHYDNVLWAFLTLFTVSTGEGWPDVLKHSVDATFEDQGPSPGYRIEISIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPENVQSFQYRMWKFVVSPPFEYAIMIMIALNTVVLMMKFHGAPDFYEAMLKHFNIVFTALFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDIVVTEINTTDRLLNLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIELNEDTSITHHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSHRACDERSGNLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIRYNDMYNLLRIISPPLGLGKNCPNRVAYKRLVKMNMPIADDNTVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSQVWPSLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLKEQQQQQQQQNATGSQGKVGALFKPILPLTHKQEKVKEKAMTNVEPPCELLPQPQSQALPRPSSTLLNNGGTLPGHDGTMKGSSSWVSEKSREVHRPRIKTPVQRGQSEDVPNLTIPQESVEMRKMDTSCSTVPGPGSGLESQGRAVSMPRLNAELQRSKSRHSPGTLLAPVPDCSPMKRSASTHAPQRPQEVNLHEHTLERPSTEERRHHHRHHHRCHHHRERDKKQRSLDRTPSTIGAPGDPAAEPSSRDRVQDRGRSHERKNHSTAGEKQRYYSCDRYSSREHCHTTKSATTSCATSPSEVQEPSINKQGSGSVKGSPVMLTSGAITPSRGRRQLPQTPLTPRPGVTFKTANSSPVHFVSAQCGSGLPTLSPGRLSRGLSEHNALLHSGTSHIPSPITRISSEPFLGYSHSDPQGQGGPYCNLRDELLSPYGARAHSPRTARPQMRPGALHLPPPPQQRRAVPNGYHFTFGGNGNASLGSGSGARPPRYYHEAEEDEWC

Target ORF information:

RefSeq Version	XM_014171608.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014171608.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTTTTGT AAAGCAAAGT 
CGAGGTGCAC TCTCTCGCAG CTCGTCCGTC TGCAGCGTTA ATTCACTGAG TTATGTCTGC 
TCCCCAGTCC ATCACTACTT GAGTAAGGAG CAGCAGAAGT CTCTGAAGAT GATGTCAGTG 
TGGGAGCAGC GCACCACTCA GCTGAGGAGA CACAACCTGC GGGCCAGCAG TGAGGCCCTG 
TACAGCGAGC TGGACCCTGA GGAGAGGCTA CGCATGTCCT CCGCCCTGCA CATCCGCCCT 
GACATGAAGA CCCACCTGGA CCGGCCCCTG GTCGTGAAGC CCCACGACGG CCCCGGGCCC 
ATGGGCCACC TCAAGCCCCA GACCCCTGAG GACACGGATA ACCCTGGGGA CCCGGCCGGC 
CCCCAGCCAC GTAGGCACCA CCGGGACAGG GAGAGACCCA TCGACGAGGC CAATGAGAAT 
GGAGATCCAG GCAAAGAGGG TAGGCACCAT GTCCATCACA GTCGCAGCAA GTACCCCAAT 
GGGACTCGGG GTAAGGAGGG GAAGAGTGAG CGGTCGCATA GCCGAGAGGG TGGCAGGAGG 
CACCACCACC AGACCTCAGT GGACGAGGGA GGTGGAGGGG GAACTGGGAC GGGAGAGAGA 
GTGCACCGCC ACCACCACTC CCACAGACAG GCCAGAGAGG GTAACAGCAC TGTCAACAGC 
GGACGCAAGG ATGAGCGCAG GTCACGCCAC AAAGATGGAC GCTCGGGAGA GCGATGCTCC 
AGGGGAGAGA ACGGGGCCAA TGGGGAGAGG AGGAGACACA AGACCCACAG TTACAGGGGC 
CAGTCCACAC TGGCGGGAGA GGAGCACAGT GAGAGGGAGA GGGGGAAGGG CCACAGGGAC 
AGATATGTTG AGTCTGAGGG CGACTCCATG GCCACTAGCA CCCAGCAGGA TCCTGGAGAT 
CAGAGGTGGG GCACAGAGAT AACTGAGGAC TCAGACAACC AGAGGAACGT CAGACGGACA 
GGATGGACGG CCGTCCACAT ACCCCCTGTC ACCATCACCT CCCTGCCTGG AGAGACCACC 
CTTATCCCCA TGAACAGTAT AGACTGTGAG ACATTGCCTA TGAACGAGGA GAAGAAGACC 
CTGGAGGTGT TGACTCGGAA CGGACCCAGA CCCATCCTCC CCTACAGCTC CATGTTTGTG 
TTTGGCCAGA CCAACATGGT GCGGCGTCTG TGTCATTACA TTGTGAGCCT GCGGTACTTT 
GAGATGTGTA TCCTGGTAGT GATATCTATG AGTAGCATTG CATTGGCTGC TGAGGACCCT 
GTCCAGGCCA ATGCTCCACG CAACAATGTG CTCAAGTATC TGGACTACGT CTTTACGGGA 
GTTTTCACAT TTGAGATGGT GATTAAGATG GTTGACCTGG GCCTTCTGCT CCATCCTGGT 
TCTTACTTCA GAGATCTCTG GAACATTCTG GACTTCATTG TGGTCAGTGG AGCACTGGTG 
GCATTTGCAT TTTCGAGCTT CATGGGACCG CCACAAGGTG TGCCCAGAGG GTCCAAAGGA 
AAGGACATCA GCACCATCAA GTCCCTCAGA GTTCTGCGAG TCTTACGGCC CCTCAAGACC 
ATCAAGCGCC TGCCAAAGCT GAAGGCTGTG TTTGACTGTG TGGTCAACTC CCTGAAGAAC 
GTCTTCAACA TCCTCATTGT CTACATGCTC TTCATGTTCA TCTTCGCTGT CATCGCTGTG 
CAGCTGTTCA AGGGAAAGTT CTTTTACTGC TCCGATGAGT CCAAGGGACT GGAAAAGGAC 
TGCAGAGGTC ATTTCCTGGA CTACGATAAG GATGAGGTGG CCGCCCAGCC TCGGGTGTGG 
AAGAAATATG AGTTCCACTA TGACAACGTG CTGTGGGCCT TCCTCACTCT CTTCACTGTG 
TCCACTGGAG AGGGCTGGCC CGATGTTCTG AAGCACTCTG TGGATGCTAC CTTTGAGGAC 
CAGGGTCCCA GTCCAGGCTA CCGTATAGAG ATCTCCATCT TCTACGTGGT CTACTTCGTG 
GTCTTTCCCT TCTTCTTCGT CAACATCTTT GTGGCCTTGA TCATCATTAC CTTCCAGGAG 
CAAGGGGACA AGGCCATGTC AGAGTGCAGC CTGGAGAAAA ACGAGAGGGC CTGCATTGAC 
TTTGCCATCA ACGCCAAGCC ACTGACGCGT TACATGCCAG AGAACGTGCA GTCCTTCCAG 
TACCGGATGT GGAAGTTTGT GGTGTCACCC CCGTTTGAGT ACGCCATTAT GATCATGATC 
GCCCTGAACA CCGTGGTGCT CATGATGAAG TTCCATGGAG CTCCAGACTT CTACGAGGCC 
ATGTTGAAGC ACTTCAACAT CGTCTTCACT GCCCTCTTCT CTCTGGAGTG CATTCTGAAG 
ATCATCGCCT TCGGACCACT GAACTACCTG AAGGATGCCT GGAACGTGTT TGACTTTGTG 
ACCGTGCTGG GAAGTATAAC TGACATCGTG GTCACAGAAA TCAATACTAC GGACCGGCTT 
TTGAACCTGA GTTTTCTGAG GCTGTTCAGA GCAGCTCGTC TCATCAAACT ACTGAGGCAA 
GGCTACACCA TCCGTATCCT GCTGTGGACC TTCGTCCAGT CCTTTAAGGC CCTGCCCTAT 
GTTTGCCTTC TGATAGCAAT GCTGTTCTTC ATCTATGCCA TCATCGGTAT GCAGGTGTTT 
GGAAACATTG AGCTCAACGA GGACACATCC ATCACTCACC ACAACAACTT CCGCACTTTT 
CTTCAGGCTC TCATGCTACT GTTCAGGAGT GCCACAGGGG AGGCGTGGCA TGAGATCATG 
CTGTCGTGTT TGAGTCACAG AGCCTGTGAT GAGAGGTCAG GCAACCTGGG GAAGGAGTGT 
GGCAGCGACT TTGCCTATTT CTACTTTGTG TCCTTCATCT TCCTCTGCTC TTTCCTGATG 
CTTAATCTGT TTGTGGCTGT GATCATGGAT AACTTTGAAT ACCTGACTCG AGACTCTTCT 
ATACTGGGGC CTCATCATCT GGATGAGTTC ATCCGTGTTT GGGCTGAGTA CGACCCTGCT 
GCCTGCGGAC GGATTCGCTA TAACGATATG TACAACTTGT TACGAATTAT CTCACCCCCC 
CTTGGCTTAG GGAAGAACTG CCCTAATAGG GTAGCATACA AGCGGCTAGT CAAGATGAAC 
ATGCCCATCG CTGATGACAA CACAGTCCAC TTCACCTCCA CACTCATGGC CCTGATCCGG 
ACGGCCCTGG AGATCAAACT GGCCTCAGGG ATGGTTGCCC AGCGTCTCTC TGATGCTGAA 
CTGAAGAAAG AGCTCTCCCA GGTTTGGCCA AGCCTCTCAC AGAAGACTAT GGATCTGTTG 
GTGACACCAC ACAAACCCAA TGAGCTTACT GTTGGGAAGG TGTATGCAGC CCTGATGATC 
TTTGACTACT ACAAGCAGAA CCGAGCCAAG AGGCTCAAGG AACAGCAACA GCAGCAGCAG 
CAACAGAATG CTACTGGCTC CCAGGGAAAG GTGGGGGCTC TGTTTAAACC AATACTGCCC 
CTGACTCACA AGCAGGAGAA AGTGAAGGAG AAGGCCATGA CCAACGTGGA GCCTCCCTGT 
GAGCTACTGC CTCAGCCCCA GTCTCAGGCC CTACCTAGAC CCAGCTCCAC CCTGCTCAAC 
AACGGAGGCA CACTGCCAGG CCATGACGGC ACCATGAAAG GATCATCCTC CTGGGTGTCA 
GAGAAGTCTA GAGAGGTCCA CAGACCCAGG ATTAAGACAC CTGTCCAAAG GGGCCAGTCT 
GAGGATGTCC CAAACCTAAC CATCCCCCAG GAGTCAGTGG AGATGAGGAA GATGGACACC 
AGTTGCAGCA CAGTGCCTGG TCCTGGGTCT GGACTGGAGA GCCAGGGAAG AGCTGTGTCC 
ATGCCCCGCC TCAATGCAGA GCTGCAGAGG AGCAAGTCGC GCCACTCCCC GGGGACCCTG 
CTGGCACCTG TTCCTGACTG CAGCCCAATG AAACGCTCTG CGTCCACCCA CGCCCCCCAG 
CGCCCCCAGG AGGTGAACCT GCATGAACAC ACCCTGGAGA GGCCCAGCAC CGAAGAAAGG 
CGTCACCATC ACCGCCACCA CCACCGCTGC CACCACCACC GAGAGAGGGA CAAGAAGCAG 
CGGTCACTGG ACAGGACGCC AAGCACCATT GGTGCACCAG GTGACCCGGC TGCAGAGCCC 
TCGTCTAGAG ACAGGGTGCA GGACCGCGGC CGCTCCCATG AGAGGAAGAA CCACTCGACG 
GCGGGGGAGA AGCAGCGCTA CTACTCTTGC GATCGCTACA GCAGCAGGGA ACACTGCCAC 
ACCACCAAAT CTGCCACCAC TAGCTGTGCC ACCTCCCCCA GCGAGGTGCA GGAACCCAGC 
ATCAACAAGC AGGGTAGTGG TTCGGTTAAA GGCAGCCCAG TGATGTTGAC CTCAGGGGCT 
ATCACACCTA GCCGTGGACG CAGGCAGCTC CCACAGACCC CCCTGACCCC CCGCCCCGGG 
GTGACGTTCA AGACTGCAAA CTCCTCCCCT GTGCACTTTG TGTCTGCCCA GTGTGGCAGC 
GGCCTGCCCA CCCTGAGTCC TGGCCGGCTG AGCCGTGGGC TGTCTGAGCA CAACGCCCTG 
CTGCATAGTG GCACCTCTCA CATCCCCTCC CCCATCACCC GCATCAGCTC TGAGCCCTTC 
CTGGGCTACA GTCACAGTGA CCCCCAAGGC CAGGGTGGGC CGTACTGCAA CCTCCGGGAC 
GAGCTGCTGA GCCCTTATGG TGCCAGAGCA CACTCCCCTC GGACTGCACG TCCTCAAATG 
AGGCCGGGGG CCCTCCACCT GCCTCCGCCA CCCCAGCAGA GACGTGCGGT GCCCAACGGA 
TACCACTTCA CCTTTGGTGG TAATGGCAAC GCCAGCCTGG GCTCTGGCTC TGGTGCCAGG 
CCACCCAGGT ACTATCACGA GGCAGAGGAG GATGAATGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106585428 ( XM_014171609.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014027084.1 Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X3, mRNA.

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CloneID	OAt67166
Clone ID Related Accession (Same CDS sequence)	XM_014171609.1
Accession Version	XM_014171609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012340658.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTTTTGT AAAGCAAAGT 
CGAGGTGCAC TCTCTCGCAG CTCGTCCGTC TGCAGCGTTA ATTCACTGAG TTATGTCTGC 
TCCCCAGTCC ATCACTACTT GAGTAAGGAG CAGCAGAAGT CTCTGAAGAT GATGTCAGTG 
TGGGAGCAGC GCACCACTCA GCTGAGGAGA CACAACCTGC GGGCCAGCAG TGAGGCCCTG 
TACAGCGAGC TGGACCCTGA GGAGAGGCTA CGCATGTCCT CCGCCCTGCA CATCCGCCCT 
GACATGAAGA CCCACCTGGA CCGGCCCCTG GTCGTGAAGC CCCACGACGG CCCCGGGCCC 
ATGGGCCACC TCAAGCCCCA GACCCCTGAG GACACGGATA ACCCTGGGGA CCCGGCCGGC 
CCCCAGCCAC GTAGGCACCA CCGGGACAGG GAGAGACCCA TCGACGAGGC CAATGAGAAT 
GGAGATCCAG GCAAAGAGGG TAGGCACCAT GTCCATCACA GTCGCAGCAA GTACCCCAAT 
GGGACTCGGG GTAAGGAGGG GAAGAGTGAG CGGTCGCATA GCCGAGAGGG TGGCAGGAGG 
CACCACCACC AGACCTCAGT GGACGAGGGA GGTGGAGGGG GAACTGGGAC GGGAGAGAGA 
GTGCACCGCC ACCACCACTC CCACAGACAG GCCAGAGAGG GTAACAGCAC TGTCAACAGC 
GGACGCAAGG ATGAGCGCAG GTCACGCCAC AAAGATGGAC GCTCGGGAGA GCGATGCTCC 
AGGGGAGAGA ACGGGGCCAA TGGGGAGAGG AGGAGACACA AGACCCACAG TTACAGGGGC 
CAGTCCACAC TGGCGGGAGA GGAGCACAGT GAGAGGGAGA GGGGGAAGGG CCACAGGGAC 
AGATATGTTG AGTCTGAGGG CGACTCCATG GCCACTAGCA CCCAGCAGGA TCCTGGAGAT 
CAGAGGTGGG GCACAGAGAT AACTGAGGAC TCAGACAACC AGAGGAACGT CAGACGGACA 
GGATGGACGG CCGTCCACAT ACCCCCTGTC ACCATCACCT CCCTGCCTGG AGAGACCACC 
CTTATCCCCA TGAACAGTAT AGACTGTGAG ACATTGCCTA TGAACGAGGA GAAGAAGACC 
CTGGAGGTGT TGACTCGGAA CGGACCCAGA CCCATCCTCC CCTACAGCTC CATGTTTGTG 
TTTGGCCAGA CCAACATGGT GCGGCGTCTG TGTCATTACA TTGTGAGCCT GCGGTACTTT 
GAGATGTGTA TCCTGGTAGT GATATCTATG AGTAGCATTG CATTGGCTGC TGAGGACCCT 
GTCCAGGCCA ATGCTCCACG CAACAATGTG CTCAAGTATC TGGACTACGT CTTTACGGGA 
GTTTTCACAT TTGAGATGGT GATTAAGATG GTTGACCTGG GCCTTCTGCT CCATCCTGGT 
TCTTACTTCA GAGATCTCTG GAACATTCTG GACTTCATTG TGGTCAGTGG AGCACTGGTG 
GCATTTGCAT TTTCACCGCC ACAAGGTGTG CCCAGAGGGT CCAAAGGAAA GGACATCAGC 
ACCATCAAGT CCCTCAGAGT TCTGCGAGTC TTACGGCCCC TCAAGACCAT CAAGCGCCTG 
CCAAAGCTGA AGGCTGTGTT TGACTGTGTG GTCAACTCCC TGAAGAACGT CTTCAACATC 
CTCATTGTCT ACATGCTCTT CATGTTCATC TTCGCTGTCA TCGCTGTGCA GCTGTTCAAG 
GGAAAGTTCT TTTACTGCTC CGATGAGTCC AAGGGACTGG AAAAGGACTG CAGAGGTCAT 
TTCCTGGACT ACGATAAGGA TGAGGTGGCC GCCCAGCCTC GGGTGTGGAA GAAATATGAG 
TTCCACTATG ACAACGTGCT GTGGGCCTTC CTCACTCTCT TCACTGTGTC CACTGGAGAG 
GGCTGGCCCG ATGTTCTGAA GCACTCTGTG GATGCTACCT TTGAGGACCA GGGTCCCAGT 
CCAGGCTACC GTATAGAGAT CTCCATCTTC TACGTGGTCT ACTTCGTGGT CTTTCCCTTC 
TTCTTCGTCA ACATCTTTGT GGCCTTGATC ATCATTACCT TCCAGGAGCA AGGGGACAAG 
GCCATGTCAG AGTGCAGCCT GGAGAAAAAC GAGAGGGCCT GCATTGACTT TGCCATCAAC 
GCCAAGCCAC TGACGCGTTA CATGCCAGAG AACGTGCAGT CCTTCCAGTA CCGGATGTGG 
AAGTTTGTGG TGTCACCCCC GTTTGAGTAC GCCATTATGA TCATGATCGC CCTGAACACC 
GTGGTGCTCA TGATGAAGTT CCATGGAGCT CCAGACTTCT ACGAGGCCAT GTTGAAGCAC 
TTCAACATCG TCTTCACTGC CCTCTTCTCT CTGGAGTGCA TTCTGAAGAT CATCGCCTTC 
GGACCACTGA ACTACCTGAA GGATGCCTGG AACGTGTTTG ACTTTGTGAC CGTGCTGGGA 
AGTATAACTG ACATCGTGGT CACAGAAATC AATACTACGG ACCGGCTTTT GAACCTGAGT 
TTTCTGAGGC TGTTCAGAGC AGCTCGTCTC ATCAAACTAC TGAGGCAAGG CTACACCATC 
CGTATCCTGC TGTGGACCTT CGTCCAGTCC TTTAAGGCCC TGCCCTATGT TTGCCTTCTG 
ATAGCAATGC TGTTCTTCAT CTATGCCATC ATCGGTATGC AGGTGTTTGG AAACATTGAG 
CTCAACGAGG ACACATCCAT CACTCACCAC AACAACTTCC GCACTTTTCT TCAGGCTCTC 
ATGCTACTGT TCAGGAGTGC CACAGGGGAG GCGTGGCATG AGATCATGCT GTCGTGTTTG 
AGTCACAGAG CCTGTGATGA GAGGTCAGGC AACCTGGGGA AGGAGTGTGG CAGCGACTTT 
GCCTATTTCT ACTTTGTGTC CTTCATCTTC CTCTGCTCTT TCCTGATGCT TAATCTGTTT 
GTGGCTGTGA TCATGGATAA CTTTGAATAC CTGACTCGAG ACTCTTCTAT ACTGGGGCCT 
CATCATCTGG ATGAGTTCAT CCGTGTTTGG GCTGAGTACG ACCCTGCTGC CTGTGGCCGT 
ATCAAGTACC TCGATATGTA TCAGATACTC CTCCACATGT CCCCACCCTT AGGTTTGGGA 
AAGAAATGTC CGCCAAGAGT CGCCTACAAG CGGCTAGTCA AGATGAACAT GCCCATCGCT 
GATGACAACA CAGTCCACTT CACCTCCACA CTCATGGCCC TGATCCGGAC GGCCCTGGAG 
ATCAAACTGG CCTCAGGGAT GGTTGCCCAG CGTCTCTCTG ATGCTGAACT GAAGAAAGAG 
CTCTCCCAGG TTTGGCCAAG CCTCTCACAG AAGACTATGG ATCTGTTGGT GACACCACAC 
AAACCCAATG AGCTTACTGT TGGGAAGGTG TATGCAGCCC TGATGATCTT TGACTACTAC 
AAGCAGAACC GAGCCAAGAG GCTCAAGGAA CAGCAACAGC AGCAGCAGCA ACAGAATGCT 
ACTGGCTCCC AGGGAAAGGT GGGGGCTCTG TTTAAACCAA TACTGCCCCT GACTCACAAG 
CAGGAGAAAG TGAAGGAGAA GGCCATGACC AACGTGGAGC CTCCCTGTGA GCTACTGCCT 
CAGCCCCAGT CTCAGGCCCT ACCTAGACCC AGCTCCACCC TGCTCAACAA CGGAGGCACA 
CTGCCAGGCC ATGACGGCAC CATGAAAGGA TCATCCTCCT GGGTGTCAGA GAAGTCTAGA 
GAGGTCCACA GACCCAGGAT TAAGACACCT GTCCAAAGGG GCCAGTCTGA GGATGTCCCA 
AACCTAACCA TCCCCCAGGA GTCAGTGGAG ATGAGGAAGA TGGACACCAG TTGCAGCACA 
GTGCCTGGTC CTGGGTCTGG ACTGGAGAGC CAGGGAAGAG CTGTGTCCAT GCCCCGCCTC 
AATGCAGAGC TGCAGAGGAG CAAGTCGCGC CACTCCCCGG GGACCCTGCT GGCACCTGTT 
CCTGACTGCA GCCCAATGAA ACGCTCTGCG TCCACCCACG CCCCCCAGCG CCCCCAGGAG 
GTGAACCTGC ATGAACACAC CCTGGAGAGG CCCAGCACCG AAGAAAGGCG TCACCATCAC 
CGCCACCACC ACCGCTGCCA CCACCACCGA GAGAGGGACA AGAAGCAGCG GTCACTGGAC 
AGGACGCCAA GCACCATTGG TGCACCAGGT GACCCGGCTG CAGAGCCCTC GTCTAGAGAC 
AGGGTGCAGG ACCGCGGCCG CTCCCATGAG AGGAAGAACC ACTCGACGGC GGGGGAGAAG 
CAGCGCTACT ACTCTTGCGA TCGCTACAGC AGCAGGGAAC ACTGCCACAC CACCAAATCT 
GCCACCACTA GCTGTGCCAC CTCCCCCAGC GAGGTGCAGG AACCCAGCAT CAACAAGCAG 
GGTAGTGGTT CGGTTAAAGG CAGCCCAGTG ATGTTGACCT CAGGGGCTAT CACACCTAGC 
CGTGGACGCA GGCAGCTCCC ACAGACCCCC CTGACCCCCC GCCCCGGGGT GACGTTCAAG 
ACTGCAAACT CCTCCCCTGT GCACTTTGTG TCTGCCCAGT GTGGCAGCGG CCTGCCCACC 
CTGAGTCCTG GCCGGCTGAG CCGTGGGCTG TCTGAGCACA ACGCCCTGCT GCATAGTGGC 
ACCTCTCACA TCCCCTCCCC CATCACCCGC ATCAGCTCTG AGCCCTTCCT GGGCTACAGT 
CACAGTGACC CCCAAGGCCA GGGTGGGCCG TACTGCAACC TCCGGGACGA GCTGCTGAGC 
CCTTATGGTG CCAGAGCACA CTCCCCTCGG ACTGCACGTC CTCAAATGAG GCCGGGGGCC 
CTCCACCTGC CTCCGCCACC CCAGCAGAGA CGTGCGGTGC CCAACGGATA CCACTTCACC 
TTTGGTGGTA ATGGCAACGC CAGCCTGGGC TCTGGCTCTG GTGCCAGGCC ACCCAGGTAC 
TATCACGAGG CAGAGGAGGA TGAATGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014027084.1
CDS	635..8047
Translation	MARFEEELSNRRGGVCQAGPPRVAGRPAGHPSGPRVYKQSMAQRARTMAIYNPIPVKQSCLTVNRSLFIFSEDCIIRKYAKKITEWPPFEYLILATIIANCIVLALEQHLPASDKTPMSERLDDTEPYFIAIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGMLATVGADFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGMLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQAAEFPCGMEAPARTCENGTVCQEYWIGPNYGITNFDNILFAILTVFQCITMEGWVDILYNANDASGNTWNWIYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKHAEEKSPLDGAWYKRKHNNPVLKRAKKSKNDLINAEEGEDHFTDVSSVGSPFTRATPPGSPFTRAAPPGSPFTRAAPPGSPFTRATPPGSPFTRATPPGSPFTRATPPGSPFTRASLKSSKNDSASYFRRKEKRLRFTIRRLVKAQSFYWTVLCLVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLTEMSMKMYGLGPRNYFHSSFNCFDFGVIIGSIFEVIWAVVKPGASYGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGMFNFEEETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESHGGVRWGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEVAITKKMALQMVKEVKEVSPMSAANISITALHSMLYTGQHGAERAMPASFVKQSRGALSRSSSVCSVNSLSYVCSPVHHYLSKEQQKSLKMMSVWEQRTTQLRRHNLRASSEALYSELDPEERLRMSSALHIRPDMKTHLDRPLVVKPHDGPGPMGHLKPQTPEDTDNPGDPAGPQPRRHHRDRERPIDEANENGDPGKEGRHHVHHSRSKYPNGTRGKEGKSERSHSREGGRRHHHQTSVDEGGGGGTGTGERVHRHHHSHRQAREGNSTVNSGRKDERRSRHKDGRSGERCSRGENGANGERRRHKTHSYRGQSTLAGEEHSERERGKGHRDRYVESEGDSMATSTQQDPGDQRWGTEITEDSDNQRNVRRTGWTAVHIPPVTITSLPGETTLIPMNSIDCETLPMNEEKKTLEVLTRNGPRPILPYSSMFVFGQTNMVRRLCHYIVSLRYFEMCILVVISMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSPPQGVPRGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVIAVQLFKGKFFYCSDESKGLEKDCRGHFLDYDKDEVAAQPRVWKKYEFHYDNVLWAFLTLFTVSTGEGWPDVLKHSVDATFEDQGPSPGYRIEISIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPENVQSFQYRMWKFVVSPPFEYAIMIMIALNTVVLMMKFHGAPDFYEAMLKHFNIVFTALFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDIVVTEINTTDRLLNLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIELNEDTSITHHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSHRACDERSGNLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYQILLHMSPPLGLGKKCPPRVAYKRLVKMNMPIADDNTVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSQVWPSLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLKEQQQQQQQQNATGSQGKVGALFKPILPLTHKQEKVKEKAMTNVEPPCELLPQPQSQALPRPSSTLLNNGGTLPGHDGTMKGSSSWVSEKSREVHRPRIKTPVQRGQSEDVPNLTIPQESVEMRKMDTSCSTVPGPGSGLESQGRAVSMPRLNAELQRSKSRHSPGTLLAPVPDCSPMKRSASTHAPQRPQEVNLHEHTLERPSTEERRHHHRHHHRCHHHRERDKKQRSLDRTPSTIGAPGDPAAEPSSRDRVQDRGRSHERKNHSTAGEKQRYYSCDRYSSREHCHTTKSATTSCATSPSEVQEPSINKQGSGSVKGSPVMLTSGAITPSRGRRQLPQTPLTPRPGVTFKTANSSPVHFVSAQCGSGLPTLSPGRLSRGLSEHNALLHSGTSHIPSPITRISSEPFLGYSHSDPQGQGGPYCNLRDELLSPYGARAHSPRTARPQMRPGALHLPPPPQQRRAVPNGYHFTFGGNGNASLGSGSGARPPRYYHEAEEDEWC

Target ORF information:

RefSeq Version	XM_014171609.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014171609.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTTTTGT AAAGCAAAGT 
CGAGGTGCAC TCTCTCGCAG CTCGTCCGTC TGCAGCGTTA ATTCACTGAG TTATGTCTGC 
TCCCCAGTCC ATCACTACTT GAGTAAGGAG CAGCAGAAGT CTCTGAAGAT GATGTCAGTG 
TGGGAGCAGC GCACCACTCA GCTGAGGAGA CACAACCTGC GGGCCAGCAG TGAGGCCCTG 
TACAGCGAGC TGGACCCTGA GGAGAGGCTA CGCATGTCCT CCGCCCTGCA CATCCGCCCT 
GACATGAAGA CCCACCTGGA CCGGCCCCTG GTCGTGAAGC CCCACGACGG CCCCGGGCCC 
ATGGGCCACC TCAAGCCCCA GACCCCTGAG GACACGGATA ACCCTGGGGA CCCGGCCGGC 
CCCCAGCCAC GTAGGCACCA CCGGGACAGG GAGAGACCCA TCGACGAGGC CAATGAGAAT 
GGAGATCCAG GCAAAGAGGG TAGGCACCAT GTCCATCACA GTCGCAGCAA GTACCCCAAT 
GGGACTCGGG GTAAGGAGGG GAAGAGTGAG CGGTCGCATA GCCGAGAGGG TGGCAGGAGG 
CACCACCACC AGACCTCAGT GGACGAGGGA GGTGGAGGGG GAACTGGGAC GGGAGAGAGA 
GTGCACCGCC ACCACCACTC CCACAGACAG GCCAGAGAGG GTAACAGCAC TGTCAACAGC 
GGACGCAAGG ATGAGCGCAG GTCACGCCAC AAAGATGGAC GCTCGGGAGA GCGATGCTCC 
AGGGGAGAGA ACGGGGCCAA TGGGGAGAGG AGGAGACACA AGACCCACAG TTACAGGGGC 
CAGTCCACAC TGGCGGGAGA GGAGCACAGT GAGAGGGAGA GGGGGAAGGG CCACAGGGAC 
AGATATGTTG AGTCTGAGGG CGACTCCATG GCCACTAGCA CCCAGCAGGA TCCTGGAGAT 
CAGAGGTGGG GCACAGAGAT AACTGAGGAC TCAGACAACC AGAGGAACGT CAGACGGACA 
GGATGGACGG CCGTCCACAT ACCCCCTGTC ACCATCACCT CCCTGCCTGG AGAGACCACC 
CTTATCCCCA TGAACAGTAT AGACTGTGAG ACATTGCCTA TGAACGAGGA GAAGAAGACC 
CTGGAGGTGT TGACTCGGAA CGGACCCAGA CCCATCCTCC CCTACAGCTC CATGTTTGTG 
TTTGGCCAGA CCAACATGGT GCGGCGTCTG TGTCATTACA TTGTGAGCCT GCGGTACTTT 
GAGATGTGTA TCCTGGTAGT GATATCTATG AGTAGCATTG CATTGGCTGC TGAGGACCCT 
GTCCAGGCCA ATGCTCCACG CAACAATGTG CTCAAGTATC TGGACTACGT CTTTACGGGA 
GTTTTCACAT TTGAGATGGT GATTAAGATG GTTGACCTGG GCCTTCTGCT CCATCCTGGT 
TCTTACTTCA GAGATCTCTG GAACATTCTG GACTTCATTG TGGTCAGTGG AGCACTGGTG 
GCATTTGCAT TTTCACCGCC ACAAGGTGTG CCCAGAGGGT CCAAAGGAAA GGACATCAGC 
ACCATCAAGT CCCTCAGAGT TCTGCGAGTC TTACGGCCCC TCAAGACCAT CAAGCGCCTG 
CCAAAGCTGA AGGCTGTGTT TGACTGTGTG GTCAACTCCC TGAAGAACGT CTTCAACATC 
CTCATTGTCT ACATGCTCTT CATGTTCATC TTCGCTGTCA TCGCTGTGCA GCTGTTCAAG 
GGAAAGTTCT TTTACTGCTC CGATGAGTCC AAGGGACTGG AAAAGGACTG CAGAGGTCAT 
TTCCTGGACT ACGATAAGGA TGAGGTGGCC GCCCAGCCTC GGGTGTGGAA GAAATATGAG 
TTCCACTATG ACAACGTGCT GTGGGCCTTC CTCACTCTCT TCACTGTGTC CACTGGAGAG 
GGCTGGCCCG ATGTTCTGAA GCACTCTGTG GATGCTACCT TTGAGGACCA GGGTCCCAGT 
CCAGGCTACC GTATAGAGAT CTCCATCTTC TACGTGGTCT ACTTCGTGGT CTTTCCCTTC 
TTCTTCGTCA ACATCTTTGT GGCCTTGATC ATCATTACCT TCCAGGAGCA AGGGGACAAG 
GCCATGTCAG AGTGCAGCCT GGAGAAAAAC GAGAGGGCCT GCATTGACTT TGCCATCAAC 
GCCAAGCCAC TGACGCGTTA CATGCCAGAG AACGTGCAGT CCTTCCAGTA CCGGATGTGG 
AAGTTTGTGG TGTCACCCCC GTTTGAGTAC GCCATTATGA TCATGATCGC CCTGAACACC 
GTGGTGCTCA TGATGAAGTT CCATGGAGCT CCAGACTTCT ACGAGGCCAT GTTGAAGCAC 
TTCAACATCG TCTTCACTGC CCTCTTCTCT CTGGAGTGCA TTCTGAAGAT CATCGCCTTC 
GGACCACTGA ACTACCTGAA GGATGCCTGG AACGTGTTTG ACTTTGTGAC CGTGCTGGGA 
AGTATAACTG ACATCGTGGT CACAGAAATC AATACTACGG ACCGGCTTTT GAACCTGAGT 
TTTCTGAGGC TGTTCAGAGC AGCTCGTCTC ATCAAACTAC TGAGGCAAGG CTACACCATC 
CGTATCCTGC TGTGGACCTT CGTCCAGTCC TTTAAGGCCC TGCCCTATGT TTGCCTTCTG 
ATAGCAATGC TGTTCTTCAT CTATGCCATC ATCGGTATGC AGGTGTTTGG AAACATTGAG 
CTCAACGAGG ACACATCCAT CACTCACCAC AACAACTTCC GCACTTTTCT TCAGGCTCTC 
ATGCTACTGT TCAGGAGTGC CACAGGGGAG GCGTGGCATG AGATCATGCT GTCGTGTTTG 
AGTCACAGAG CCTGTGATGA GAGGTCAGGC AACCTGGGGA AGGAGTGTGG CAGCGACTTT 
GCCTATTTCT ACTTTGTGTC CTTCATCTTC CTCTGCTCTT TCCTGATGCT TAATCTGTTT 
GTGGCTGTGA TCATGGATAA CTTTGAATAC CTGACTCGAG ACTCTTCTAT ACTGGGGCCT 
CATCATCTGG ATGAGTTCAT CCGTGTTTGG GCTGAGTACG ACCCTGCTGC CTGTGGCCGT 
ATCAAGTACC TCGATATGTA TCAGATACTC CTCCACATGT CCCCACCCTT AGGTTTGGGA 
AAGAAATGTC CGCCAAGAGT CGCCTACAAG CGGCTAGTCA AGATGAACAT GCCCATCGCT 
GATGACAACA CAGTCCACTT CACCTCCACA CTCATGGCCC TGATCCGGAC GGCCCTGGAG 
ATCAAACTGG CCTCAGGGAT GGTTGCCCAG CGTCTCTCTG ATGCTGAACT GAAGAAAGAG 
CTCTCCCAGG TTTGGCCAAG CCTCTCACAG AAGACTATGG ATCTGTTGGT GACACCACAC 
AAACCCAATG AGCTTACTGT TGGGAAGGTG TATGCAGCCC TGATGATCTT TGACTACTAC 
AAGCAGAACC GAGCCAAGAG GCTCAAGGAA CAGCAACAGC AGCAGCAGCA ACAGAATGCT 
ACTGGCTCCC AGGGAAAGGT GGGGGCTCTG TTTAAACCAA TACTGCCCCT GACTCACAAG 
CAGGAGAAAG TGAAGGAGAA GGCCATGACC AACGTGGAGC CTCCCTGTGA GCTACTGCCT 
CAGCCCCAGT CTCAGGCCCT ACCTAGACCC AGCTCCACCC TGCTCAACAA CGGAGGCACA 
CTGCCAGGCC ATGACGGCAC CATGAAAGGA TCATCCTCCT GGGTGTCAGA GAAGTCTAGA 
GAGGTCCACA GACCCAGGAT TAAGACACCT GTCCAAAGGG GCCAGTCTGA GGATGTCCCA 
AACCTAACCA TCCCCCAGGA GTCAGTGGAG ATGAGGAAGA TGGACACCAG TTGCAGCACA 
GTGCCTGGTC CTGGGTCTGG ACTGGAGAGC CAGGGAAGAG CTGTGTCCAT GCCCCGCCTC 
AATGCAGAGC TGCAGAGGAG CAAGTCGCGC CACTCCCCGG GGACCCTGCT GGCACCTGTT 
CCTGACTGCA GCCCAATGAA ACGCTCTGCG TCCACCCACG CCCCCCAGCG CCCCCAGGAG 
GTGAACCTGC ATGAACACAC CCTGGAGAGG CCCAGCACCG AAGAAAGGCG TCACCATCAC 
CGCCACCACC ACCGCTGCCA CCACCACCGA GAGAGGGACA AGAAGCAGCG GTCACTGGAC 
AGGACGCCAA GCACCATTGG TGCACCAGGT GACCCGGCTG CAGAGCCCTC GTCTAGAGAC 
AGGGTGCAGG ACCGCGGCCG CTCCCATGAG AGGAAGAACC ACTCGACGGC GGGGGAGAAG 
CAGCGCTACT ACTCTTGCGA TCGCTACAGC AGCAGGGAAC ACTGCCACAC CACCAAATCT 
GCCACCACTA GCTGTGCCAC CTCCCCCAGC GAGGTGCAGG AACCCAGCAT CAACAAGCAG 
GGTAGTGGTT CGGTTAAAGG CAGCCCAGTG ATGTTGACCT CAGGGGCTAT CACACCTAGC 
CGTGGACGCA GGCAGCTCCC ACAGACCCCC CTGACCCCCC GCCCCGGGGT GACGTTCAAG 
ACTGCAAACT CCTCCCCTGT GCACTTTGTG TCTGCCCAGT GTGGCAGCGG CCTGCCCACC 
CTGAGTCCTG GCCGGCTGAG CCGTGGGCTG TCTGAGCACA ACGCCCTGCT GCATAGTGGC 
ACCTCTCACA TCCCCTCCCC CATCACCCGC ATCAGCTCTG AGCCCTTCCT GGGCTACAGT 
CACAGTGACC CCCAAGGCCA GGGTGGGCCG TACTGCAACC TCCGGGACGA GCTGCTGAGC 
CCTTATGGTG CCAGAGCACA CTCCCCTCGG ACTGCACGTC CTCAAATGAG GCCGGGGGCC 
CTCCACCTGC CTCCGCCACC CCAGCAGAGA CGTGCGGTGC CCAACGGATA CCACTTCACC 
TTTGGTGGTA ATGGCAACGC CAGCCTGGGC TCTGGCTCTG GTGCCAGGCC ACCCAGGTAC 
TATCACGAGG CAGAGGAGGA TGAATGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106585428 ( XM_014171610.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014027085.1 Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X4, mRNA.

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CloneID	OAt67167
Clone ID Related Accession (Same CDS sequence)	XM_014171610.1
Accession Version	XM_014171610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012340658.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTTTTGT AAAGCAAAGT 
CGAGGTGCAC TCTCTCGCAG CTCGTCCGTC TGCAGCGTTA ATTCACTTAA GGAGCAGCAG 
AAGTCTCTGA AGATGATGTC AGTGTGGGAG CAGCGCACCA CTCAGCTGAG GAGACACAAC 
CTGCGGGCCA GCAGTGAGGC CCTGTACAGC GAGCTGGACC CTGAGGAGAG GCTACGCATG 
TCCTCCGCCC TGCACATCCG CCCTGACATG AAGACCCACC TGGACCGGCC CCTGGTCGTG 
AAGCCCCACG ACGGCCCCGG GCCCATGGGC CACCTCAAGC CCCAGACCCC TGAGGACACG 
GATAACCCTG GGGACCCGGC CGGCCCCCAG CCACGTAGGC ACCACCGGGA CAGGGAGAGA 
CCCATCGACG AGGCCAATGA GAATGGAGAT CCAGGCAAAG AGGGTAGGCA CCATGTCCAT 
CACAGTCGCA GCAAGTACCC CAATGGGACT CGGGGTAAGG AGGGGAAGAG TGAGCGGTCG 
CATAGCCGAG AGGGTGGCAG GAGGCACCAC CACCAGACCT CAGTGGACGA GGGAGGTGGA 
GGGGGAACTG GGACGGGAGA GAGAGTGCAC CGCCACCACC ACTCCCACAG ACAGGCCAGA 
GAGGGTAACA GCACTGTCAA CAGCGGACGC AAGGATGAGC GCAGGTCACG CCACAAAGAT 
GGACGCTCGG GAGAGCGATG CTCCAGGGGA GAGAACGGGG CCAATGGGGA GAGGAGGAGA 
CACAAGACCC ACAGTTACAG GGGCCAGTCC ACACTGGCGG GAGAGGAGCA CAGTGAGAGG 
GAGAGGGGGA AGGGCCACAG GGACAGATAT GTTGAGTCTG AGGGCGACTC CATGGCCACT 
AGCACCCAGC AGGATCCTGG AGATCAGAGG TGGGGCACAG AGATAACTGA GGACTCAGAC 
AACCAGAGGA ACGTCAGACG GACAGGATGG ACGGCCGTCC ACATACCCCC TGTCACCATC 
ACCTCCCTGC CTGGAGAGAC CACCCTTATC CCCATGAACA GTATAGACTG TGAGACATTG 
CCTATGAACG AGGAGAAGAA GACCCTGGAG GTGTTGACTC GGAACGGACC CAGACCCATC 
CTCCCCTACA GCTCCATGTT TGTGTTTGGC CAGACCAACA TGGTGCGGCG TCTGTGTCAT 
TACATTGTGA GCCTGCGGTA CTTTGAGATG TGTATCCTGG TAGTGATATC TATGAGTAGC 
ATTGCATTGG CTGCTGAGGA CCCTGTCCAG GCCAATGCTC CACGCAACAA TGTGCTCAAG 
TATCTGGACT ACGTCTTTAC GGGAGTTTTC ACATTTGAGA TGGTGATTAA GATGGTTGAC 
CTGGGCCTTC TGCTCCATCC TGGTTCTTAC TTCAGAGATC TCTGGAACAT TCTGGACTTC 
ATTGTGGTCA GTGGAGCACT GGTGGCATTT GCATTTTCGA GCTTCATGGG ACCGCCACAA 
GGTGTGCCCA GAGGGTCCAA AGGAAAGGAC ATCAGCACCA TCAAGTCCCT CAGAGTTCTG 
CGAGTCTTAC GGCCCCTCAA GACCATCAAG CGCCTGCCAA AGCTGAAGGC TGTGTTTGAC 
TGTGTGGTCA ACTCCCTGAA GAACGTCTTC AACATCCTCA TTGTCTACAT GCTCTTCATG 
TTCATCTTCG CTGTCATCGC TGTGCAGCTG TTCAAGGGAA AGTTCTTTTA CTGCTCCGAT 
GAGTCCAAGG GACTGGAAAA GGACTGCAGA GGTCATTTCC TGGACTACGA TAAGGATGAG 
GTGGCCGCCC AGCCTCGGGT GTGGAAGAAA TATGAGTTCC ACTATGACAA CGTGCTGTGG 
GCCTTCCTCA CTCTCTTCAC TGTGTCCACT GGAGAGGGCT GGCCCGATGT TCTGAAGCAC 
TCTGTGGATG CTACCTTTGA GGACCAGGGT CCCAGTCCAG GCTACCGTAT AGAGATCTCC 
ATCTTCTACG TGGTCTACTT CGTGGTCTTT CCCTTCTTCT TCGTCAACAT CTTTGTGGCC 
TTGATCATCA TTACCTTCCA GGAGCAAGGG GACAAGGCCA TGTCAGAGTG CAGCCTGGAG 
AAAAACGAGA GGGCCTGCAT TGACTTTGCC ATCAACGCCA AGCCACTGAC GCGTTACATG 
CCAGAGAACG TGCAGTCCTT CCAGTACCGG ATGTGGAAGT TTGTGGTGTC ACCCCCGTTT 
GAGTACGCCA TTATGATCAT GATCGCCCTG AACACCGTGG TGCTCATGAT GAAGTTCCAT 
GGAGCTCCAG ACTTCTACGA GGCCATGTTG AAGCACTTCA ACATCGTCTT CACTGCCCTC 
TTCTCTCTGG AGTGCATTCT GAAGATCATC GCCTTCGGAC CACTGAACTA CCTGAAGGAT 
GCCTGGAACG TGTTTGACTT TGTGACCGTG CTGGGAAGTA TAACTGACAT CGTGGTCACA 
GAAATCAATA CTACGGACCG GCTTTTGAAC CTGAGTTTTC TGAGGCTGTT CAGAGCAGCT 
CGTCTCATCA AACTACTGAG GCAAGGCTAC ACCATCCGTA TCCTGCTGTG GACCTTCGTC 
CAGTCCTTTA AGGCCCTGCC CTATGTTTGC CTTCTGATAG CAATGCTGTT CTTCATCTAT 
GCCATCATCG GTATGCAGGT GTTTGGAAAC ATTGAGCTCA ACGAGGACAC ATCCATCACT 
CACCACAACA ACTTCCGCAC TTTTCTTCAG GCTCTCATGC TACTGTTCAG GAGTGCCACA 
GGGGAGGCGT GGCATGAGAT CATGCTGTCG TGTTTGAGTC ACAGAGCCTG TGATGAGAGG 
TCAGGCAACC TGGGGAAGGA GTGTGGCAGC GACTTTGCCT ATTTCTACTT TGTGTCCTTC 
ATCTTCCTCT GCTCTTTCCT GATGCTTAAT CTGTTTGTGG CTGTGATCAT GGATAACTTT 
GAATACCTGA CTCGAGACTC TTCTATACTG GGGCCTCATC ATCTGGATGA GTTCATCCGT 
GTTTGGGCTG AGTACGACCC TGCTGCCTGT GGCCGTATCA AGTACCTCGA TATGTATCAG 
ATACTCCTCC ACATGTCCCC ACCCTTAGGT TTGGGAAAGA AATGTCCGCC AAGAGTCGCC 
TACAAGCGGC TAGTCAAGAT GAACATGCCC ATCGCTGATG ACAACACAGT CCACTTCACC 
TCCACACTCA TGGCCCTGAT CCGGACGGCC CTGGAGATCA AACTGGCCTC AGGGATGGTT 
GCCCAGCGTC TCTCTGATGC TGAACTGAAG AAAGAGCTCT CCCAGGTTTG GCCAAGCCTC 
TCACAGAAGA CTATGGATCT GTTGGTGACA CCACACAAAC CCAATGAGCT TACTGTTGGG 
AAGGTGTATG CAGCCCTGAT GATCTTTGAC TACTACAAGC AGAACCGAGC CAAGAGGCTC 
AAGGAACAGC AACAGCAGCA GCAGCAACAG AATGCTACTG GCTCCCAGGG AAAGGTGGGG 
GCTCTGTTTA AACCAATACT GCCCCTGACT CACAAGCAGG AGAAAGTGAA GGAGAAGGCC 
ATGACCAACG TGGAGCCTCC CTGTGAGCTA CTGCCTCAGC CCCAGTCTCA GGCCCTACCT 
AGACCCAGCT CCACCCTGCT CAACAACGGA GGCACACTGC CAGGCCATGA CGGCACCATG 
AAAGGATCAT CCTCCTGGGT GTCAGAGAAG TCTAGAGAGG TCCACAGACC CAGGATTAAG 
ACACCTGTCC AAAGGGGCCA GTCTGAGGAT GTCCCAAACC TAACCATCCC CCAGGAGTCA 
GTGGAGATGA GGAAGATGGA CACCAGTTGC AGCACAGTGC CTGGTCCTGG GTCTGGACTG 
GAGAGCCAGG GAAGAGCTGT GTCCATGCCC CGCCTCAATG CAGAGCTGCA GAGGAGCAAG 
TCGCGCCACT CCCCGGGGAC CCTGCTGGCA CCTGTTCCTG ACTGCAGCCC AATGAAACGC 
TCTGCGTCCA CCCACGCCCC CCAGCGCCCC CAGGAGGTGA ACCTGCATGA ACACACCCTG 
GAGAGGCCCA GCACCGAAGA AAGGCGTCAC CATCACCGCC ACCACCACCG CTGCCACCAC 
CACCGAGAGA GGGACAAGAA GCAGCGGTCA CTGGACAGGA CGCCAAGCAC CATTGGTGCA 
CCAGGTGACC CGGCTGCAGA GCCCTCGTCT AGAGACAGGG TGCAGGACCG CGGCCGCTCC 
CATGAGAGGA AGAACCACTC GACGGCGGGG GAGAAGCAGC GCTACTACTC TTGCGATCGC 
TACAGCAGCA GGGAACACTG CCACACCACC AAATCTGCCA CCACTAGCTG TGCCACCTCC 
CCCAGCGAGG TGCAGGAACC CAGCATCAAC AAGCAGGGTA GTGGTTCGGT TAAAGGCAGC 
CCAGTGATGT TGACCTCAGG GGCTATCACA CCTAGCCGTG GACGCAGGCA GCTCCCACAG 
ACCCCCCTGA CCCCCCGCCC CGGGGTGACG TTCAAGACTG CAAACTCCTC CCCTGTGCAC 
TTTGTGTCTG CCCAGTGTGG CAGCGGCCTG CCCACCCTGA GTCCTGGCCG GCTGAGCCGT 
GGGCTGTCTG AGCACAACGC CCTGCTGCAT AGTGGCACCT CTCACATCCC CTCCCCCATC 
ACCCGCATCA GCTCTGAGCC CTTCCTGGGC TACAGTCACA GTGACCCCCA AGGCCAGGGT 
GGGCCGTACT GCAACCTCCG GGACGAGCTG CTGAGCCCTT ATGGTGCCAG AGCACACTCC 
CCTCGGACTG CACGTCCTCA AATGAGGCCG GGGGCCCTCC ACCTGCCTCC GCCACCCCAG 
CAGAGACGTG CGGTGCCCAA CGGATACCAC TTCACCTTTG GTGGTAATGG CAACGCCAGC 
CTGGGCTCTG GCTCTGGTGC CAGGCCACCC AGGTACTATC ACGAGGCAGA GGAGGATGAA 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014027085.1
CDS	635..8023
Translation	MARFEEELSNRRGGVCQAGPPRVAGRPAGHPSGPRVYKQSMAQRARTMAIYNPIPVKQSCLTVNRSLFIFSEDCIIRKYAKKITEWPPFEYLILATIIANCIVLALEQHLPASDKTPMSERLDDTEPYFIAIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGMLATVGADFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGMLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQAAEFPCGMEAPARTCENGTVCQEYWIGPNYGITNFDNILFAILTVFQCITMEGWVDILYNANDASGNTWNWIYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKHAEEKSPLDGAWYKRKHNNPVLKRAKKSKNDLINAEEGEDHFTDVSSVGSPFTRATPPGSPFTRAAPPGSPFTRAAPPGSPFTRATPPGSPFTRATPPGSPFTRATPPGSPFTRASLKSSKNDSASYFRRKEKRLRFTIRRLVKAQSFYWTVLCLVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLTEMSMKMYGLGPRNYFHSSFNCFDFGVIIGSIFEVIWAVVKPGASYGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGMFNFEEETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESHGGVRWGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEVAITKKMALQMVKEVKEVSPMSAANISITALHSMLYTGQHGAERAMPASFVKQSRGALSRSSSVCSVNSLKEQQKSLKMMSVWEQRTTQLRRHNLRASSEALYSELDPEERLRMSSALHIRPDMKTHLDRPLVVKPHDGPGPMGHLKPQTPEDTDNPGDPAGPQPRRHHRDRERPIDEANENGDPGKEGRHHVHHSRSKYPNGTRGKEGKSERSHSREGGRRHHHQTSVDEGGGGGTGTGERVHRHHHSHRQAREGNSTVNSGRKDERRSRHKDGRSGERCSRGENGANGERRRHKTHSYRGQSTLAGEEHSERERGKGHRDRYVESEGDSMATSTQQDPGDQRWGTEITEDSDNQRNVRRTGWTAVHIPPVTITSLPGETTLIPMNSIDCETLPMNEEKKTLEVLTRNGPRPILPYSSMFVFGQTNMVRRLCHYIVSLRYFEMCILVVISMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGPPQGVPRGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVIAVQLFKGKFFYCSDESKGLEKDCRGHFLDYDKDEVAAQPRVWKKYEFHYDNVLWAFLTLFTVSTGEGWPDVLKHSVDATFEDQGPSPGYRIEISIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPENVQSFQYRMWKFVVSPPFEYAIMIMIALNTVVLMMKFHGAPDFYEAMLKHFNIVFTALFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDIVVTEINTTDRLLNLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIELNEDTSITHHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSHRACDERSGNLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYQILLHMSPPLGLGKKCPPRVAYKRLVKMNMPIADDNTVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSQVWPSLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLKEQQQQQQQQNATGSQGKVGALFKPILPLTHKQEKVKEKAMTNVEPPCELLPQPQSQALPRPSSTLLNNGGTLPGHDGTMKGSSSWVSEKSREVHRPRIKTPVQRGQSEDVPNLTIPQESVEMRKMDTSCSTVPGPGSGLESQGRAVSMPRLNAELQRSKSRHSPGTLLAPVPDCSPMKRSASTHAPQRPQEVNLHEHTLERPSTEERRHHHRHHHRCHHHRERDKKQRSLDRTPSTIGAPGDPAAEPSSRDRVQDRGRSHERKNHSTAGEKQRYYSCDRYSSREHCHTTKSATTSCATSPSEVQEPSINKQGSGSVKGSPVMLTSGAITPSRGRRQLPQTPLTPRPGVTFKTANSSPVHFVSAQCGSGLPTLSPGRLSRGLSEHNALLHSGTSHIPSPITRISSEPFLGYSHSDPQGQGGPYCNLRDELLSPYGARAHSPRTARPQMRPGALHLPPPPQQRRAVPNGYHFTFGGNGNASLGSGSGARPPRYYHEAEEDEWC

Target ORF information:

RefSeq Version	XM_014171610.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014171610.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTTTTGT AAAGCAAAGT 
CGAGGTGCAC TCTCTCGCAG CTCGTCCGTC TGCAGCGTTA ATTCACTTAA GGAGCAGCAG 
AAGTCTCTGA AGATGATGTC AGTGTGGGAG CAGCGCACCA CTCAGCTGAG GAGACACAAC 
CTGCGGGCCA GCAGTGAGGC CCTGTACAGC GAGCTGGACC CTGAGGAGAG GCTACGCATG 
TCCTCCGCCC TGCACATCCG CCCTGACATG AAGACCCACC TGGACCGGCC CCTGGTCGTG 
AAGCCCCACG ACGGCCCCGG GCCCATGGGC CACCTCAAGC CCCAGACCCC TGAGGACACG 
GATAACCCTG GGGACCCGGC CGGCCCCCAG CCACGTAGGC ACCACCGGGA CAGGGAGAGA 
CCCATCGACG AGGCCAATGA GAATGGAGAT CCAGGCAAAG AGGGTAGGCA CCATGTCCAT 
CACAGTCGCA GCAAGTACCC CAATGGGACT CGGGGTAAGG AGGGGAAGAG TGAGCGGTCG 
CATAGCCGAG AGGGTGGCAG GAGGCACCAC CACCAGACCT CAGTGGACGA GGGAGGTGGA 
GGGGGAACTG GGACGGGAGA GAGAGTGCAC CGCCACCACC ACTCCCACAG ACAGGCCAGA 
GAGGGTAACA GCACTGTCAA CAGCGGACGC AAGGATGAGC GCAGGTCACG CCACAAAGAT 
GGACGCTCGG GAGAGCGATG CTCCAGGGGA GAGAACGGGG CCAATGGGGA GAGGAGGAGA 
CACAAGACCC ACAGTTACAG GGGCCAGTCC ACACTGGCGG GAGAGGAGCA CAGTGAGAGG 
GAGAGGGGGA AGGGCCACAG GGACAGATAT GTTGAGTCTG AGGGCGACTC CATGGCCACT 
AGCACCCAGC AGGATCCTGG AGATCAGAGG TGGGGCACAG AGATAACTGA GGACTCAGAC 
AACCAGAGGA ACGTCAGACG GACAGGATGG ACGGCCGTCC ACATACCCCC TGTCACCATC 
ACCTCCCTGC CTGGAGAGAC CACCCTTATC CCCATGAACA GTATAGACTG TGAGACATTG 
CCTATGAACG AGGAGAAGAA GACCCTGGAG GTGTTGACTC GGAACGGACC CAGACCCATC 
CTCCCCTACA GCTCCATGTT TGTGTTTGGC CAGACCAACA TGGTGCGGCG TCTGTGTCAT 
TACATTGTGA GCCTGCGGTA CTTTGAGATG TGTATCCTGG TAGTGATATC TATGAGTAGC 
ATTGCATTGG CTGCTGAGGA CCCTGTCCAG GCCAATGCTC CACGCAACAA TGTGCTCAAG 
TATCTGGACT ACGTCTTTAC GGGAGTTTTC ACATTTGAGA TGGTGATTAA GATGGTTGAC 
CTGGGCCTTC TGCTCCATCC TGGTTCTTAC TTCAGAGATC TCTGGAACAT TCTGGACTTC 
ATTGTGGTCA GTGGAGCACT GGTGGCATTT GCATTTTCGA GCTTCATGGG ACCGCCACAA 
GGTGTGCCCA GAGGGTCCAA AGGAAAGGAC ATCAGCACCA TCAAGTCCCT CAGAGTTCTG 
CGAGTCTTAC GGCCCCTCAA GACCATCAAG CGCCTGCCAA AGCTGAAGGC TGTGTTTGAC 
TGTGTGGTCA ACTCCCTGAA GAACGTCTTC AACATCCTCA TTGTCTACAT GCTCTTCATG 
TTCATCTTCG CTGTCATCGC TGTGCAGCTG TTCAAGGGAA AGTTCTTTTA CTGCTCCGAT 
GAGTCCAAGG GACTGGAAAA GGACTGCAGA GGTCATTTCC TGGACTACGA TAAGGATGAG 
GTGGCCGCCC AGCCTCGGGT GTGGAAGAAA TATGAGTTCC ACTATGACAA CGTGCTGTGG 
GCCTTCCTCA CTCTCTTCAC TGTGTCCACT GGAGAGGGCT GGCCCGATGT TCTGAAGCAC 
TCTGTGGATG CTACCTTTGA GGACCAGGGT CCCAGTCCAG GCTACCGTAT AGAGATCTCC 
ATCTTCTACG TGGTCTACTT CGTGGTCTTT CCCTTCTTCT TCGTCAACAT CTTTGTGGCC 
TTGATCATCA TTACCTTCCA GGAGCAAGGG GACAAGGCCA TGTCAGAGTG CAGCCTGGAG 
AAAAACGAGA GGGCCTGCAT TGACTTTGCC ATCAACGCCA AGCCACTGAC GCGTTACATG 
CCAGAGAACG TGCAGTCCTT CCAGTACCGG ATGTGGAAGT TTGTGGTGTC ACCCCCGTTT 
GAGTACGCCA TTATGATCAT GATCGCCCTG AACACCGTGG TGCTCATGAT GAAGTTCCAT 
GGAGCTCCAG ACTTCTACGA GGCCATGTTG AAGCACTTCA ACATCGTCTT CACTGCCCTC 
TTCTCTCTGG AGTGCATTCT GAAGATCATC GCCTTCGGAC CACTGAACTA CCTGAAGGAT 
GCCTGGAACG TGTTTGACTT TGTGACCGTG CTGGGAAGTA TAACTGACAT CGTGGTCACA 
GAAATCAATA CTACGGACCG GCTTTTGAAC CTGAGTTTTC TGAGGCTGTT CAGAGCAGCT 
CGTCTCATCA AACTACTGAG GCAAGGCTAC ACCATCCGTA TCCTGCTGTG GACCTTCGTC 
CAGTCCTTTA AGGCCCTGCC CTATGTTTGC CTTCTGATAG CAATGCTGTT CTTCATCTAT 
GCCATCATCG GTATGCAGGT GTTTGGAAAC ATTGAGCTCA ACGAGGACAC ATCCATCACT 
CACCACAACA ACTTCCGCAC TTTTCTTCAG GCTCTCATGC TACTGTTCAG GAGTGCCACA 
GGGGAGGCGT GGCATGAGAT CATGCTGTCG TGTTTGAGTC ACAGAGCCTG TGATGAGAGG 
TCAGGCAACC TGGGGAAGGA GTGTGGCAGC GACTTTGCCT ATTTCTACTT TGTGTCCTTC 
ATCTTCCTCT GCTCTTTCCT GATGCTTAAT CTGTTTGTGG CTGTGATCAT GGATAACTTT 
GAATACCTGA CTCGAGACTC TTCTATACTG GGGCCTCATC ATCTGGATGA GTTCATCCGT 
GTTTGGGCTG AGTACGACCC TGCTGCCTGT GGCCGTATCA AGTACCTCGA TATGTATCAG 
ATACTCCTCC ACATGTCCCC ACCCTTAGGT TTGGGAAAGA AATGTCCGCC AAGAGTCGCC 
TACAAGCGGC TAGTCAAGAT GAACATGCCC ATCGCTGATG ACAACACAGT CCACTTCACC 
TCCACACTCA TGGCCCTGAT CCGGACGGCC CTGGAGATCA AACTGGCCTC AGGGATGGTT 
GCCCAGCGTC TCTCTGATGC TGAACTGAAG AAAGAGCTCT CCCAGGTTTG GCCAAGCCTC 
TCACAGAAGA CTATGGATCT GTTGGTGACA CCACACAAAC CCAATGAGCT TACTGTTGGG 
AAGGTGTATG CAGCCCTGAT GATCTTTGAC TACTACAAGC AGAACCGAGC CAAGAGGCTC 
AAGGAACAGC AACAGCAGCA GCAGCAACAG AATGCTACTG GCTCCCAGGG AAAGGTGGGG 
GCTCTGTTTA AACCAATACT GCCCCTGACT CACAAGCAGG AGAAAGTGAA GGAGAAGGCC 
ATGACCAACG TGGAGCCTCC CTGTGAGCTA CTGCCTCAGC CCCAGTCTCA GGCCCTACCT 
AGACCCAGCT CCACCCTGCT CAACAACGGA GGCACACTGC CAGGCCATGA CGGCACCATG 
AAAGGATCAT CCTCCTGGGT GTCAGAGAAG TCTAGAGAGG TCCACAGACC CAGGATTAAG 
ACACCTGTCC AAAGGGGCCA GTCTGAGGAT GTCCCAAACC TAACCATCCC CCAGGAGTCA 
GTGGAGATGA GGAAGATGGA CACCAGTTGC AGCACAGTGC CTGGTCCTGG GTCTGGACTG 
GAGAGCCAGG GAAGAGCTGT GTCCATGCCC CGCCTCAATG CAGAGCTGCA GAGGAGCAAG 
TCGCGCCACT CCCCGGGGAC CCTGCTGGCA CCTGTTCCTG ACTGCAGCCC AATGAAACGC 
TCTGCGTCCA CCCACGCCCC CCAGCGCCCC CAGGAGGTGA ACCTGCATGA ACACACCCTG 
GAGAGGCCCA GCACCGAAGA AAGGCGTCAC CATCACCGCC ACCACCACCG CTGCCACCAC 
CACCGAGAGA GGGACAAGAA GCAGCGGTCA CTGGACAGGA CGCCAAGCAC CATTGGTGCA 
CCAGGTGACC CGGCTGCAGA GCCCTCGTCT AGAGACAGGG TGCAGGACCG CGGCCGCTCC 
CATGAGAGGA AGAACCACTC GACGGCGGGG GAGAAGCAGC GCTACTACTC TTGCGATCGC 
TACAGCAGCA GGGAACACTG CCACACCACC AAATCTGCCA CCACTAGCTG TGCCACCTCC 
CCCAGCGAGG TGCAGGAACC CAGCATCAAC AAGCAGGGTA GTGGTTCGGT TAAAGGCAGC 
CCAGTGATGT TGACCTCAGG GGCTATCACA CCTAGCCGTG GACGCAGGCA GCTCCCACAG 
ACCCCCCTGA CCCCCCGCCC CGGGGTGACG TTCAAGACTG CAAACTCCTC CCCTGTGCAC 
TTTGTGTCTG CCCAGTGTGG CAGCGGCCTG CCCACCCTGA GTCCTGGCCG GCTGAGCCGT 
GGGCTGTCTG AGCACAACGC CCTGCTGCAT AGTGGCACCT CTCACATCCC CTCCCCCATC 
ACCCGCATCA GCTCTGAGCC CTTCCTGGGC TACAGTCACA GTGACCCCCA AGGCCAGGGT 
GGGCCGTACT GCAACCTCCG GGACGAGCTG CTGAGCCCTT ATGGTGCCAG AGCACACTCC 
CCTCGGACTG CACGTCCTCA AATGAGGCCG GGGGCCCTCC ACCTGCCTCC GCCACCCCAG 
CAGAGACGTG CGGTGCCCAA CGGATACCAC TTCACCTTTG GTGGTAATGG CAACGCCAGC 
CTGGGCTCTG GCTCTGGTGC CAGGCCACCC AGGTACTATC ACGAGGCAGA GGAGGATGAA 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106585428 ( XM_014171611.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014027086.1 Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X5, mRNA.

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CloneID	OAt67168
Clone ID Related Accession (Same CDS sequence)	XM_014171611.1
Accession Version	XM_014171611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012340658.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTTTTGT AAAGCAAAGT 
CGAGGTGCAC TCTCTCGCAG CTCGTCCGTC TGCAGCGTTA ATTCACTGAG TTATGTCTGC 
TCCCCAGTCC ATCACTACTT GAGTAAGGAG CAGCAGAAGT CTCTGAAGAT GATGTCAGTG 
TGGGAGCAGC GCACCACTCA GCTGAGGAGA CACAACCTGC GGGCCAGCAG TGAGGCCCTG 
TACAGCGAGC TGGACCCTGA GGAGAGGCTA CGCATGTCCT CCGCCCTGCA CATCCGCCCT 
GACATGAAGA CCCACCTGGA CCGGCCCCTG GTCGTGAAGC CCCACGACGG CCCCGGGCCC 
ATGGGCCACC TCAAGCCCCA GACCCCTGAG GACACGGATA ACCCTGGGGA CCCGGCCGGC 
CCCCAGCCAC GTAGGCACCA CCGGGACAGG GAGAGACCCA TCGACGAGGC CAATGAGAAT 
GGAGATCCAG GCAAAGAGGG TAGGCACCAT GTCCATCACA GTCGCAGCAA GTACCCCAAT 
GGGACTCGGG GTAAGGAGGG GAAGAGTGAG CGGTCGCATA GCCGAGAGGG TGGCAGGAGG 
CACCACCACC AGACCTCAGT GGACGAGGGA GGTGGAGGGG GAACTGGGAC GGGAGAGAGA 
GTGCACCGCC ACCACCACTC CCACAGACAG GCCAGAGAGG GTAACAGCAC TGTCAACAGC 
GGACGCAAGG ATGAGCGCAG GTCACGCCAC AAAGATGGAC GCTCGGGAGA GCGATGCTCC 
AGGGGAGAGA ACGGGGCCAA TGGGGAGAGG AGGAGACACA AGACCCACAG TTACAGGGGC 
CAGTCCACAC TGGCGGGAGA GGAGCACAGT GAGAGGGAGA GGGGGAAGGG CCACAGGGAC 
AGATATGTTG AGTCTGAGGG CGACTCCATG GCCACTAGCA CCCAGCAGGA TCCTGGAGAT 
CAGAGGTGGG GCACAGAGAT AACTGAGGAC TCAGACAACC AGAGGAACGT CAGACGGACA 
GGATGGACGG CCGTCCACAT ACCCCCTGTC ACCATCACCT CCCTGCCTGG AGAGACCACC 
CTTATCCCCA TGAACAGTAT AGACTGTGAG ACATTGCCTA TGAACGAGGA GAAGAAGACC 
CTGGAGGTGT TGACTCGGAA CGGACCCAGA CCCATCCTCC CCTACAGCTC CATGTTTGTG 
TTTGGCCAGA CCAACATGGT GCGGCGTCTG TGTCATTACA TTGTGAGCCT GCGGTACTTT 
GAGATGTGTA TCCTGGTAGT GATATCTATG AGTAGCATTG CATTGGCTGC TGAGGACCCT 
GTCCAGGCCA ATGCTCCACG CAACAATGTG CTCAAGTATC TGGACTACGT CTTTACGGGA 
GTTTTCACAT TTGAGATGGT GATTAAGATG GTTGACCTGG GCCTTCTGCT CCATCCTGGT 
TCTTACTTCA GAGATCTCTG GAACATTCTG GACTTCATTG TGGTCAGTGG AGCACTGGTG 
GCATTTGCAT TTTCGAGCTT CATGGGACCG CCACAAGGTG TGCCCAGAGG GTCCAAAGGA 
AAGGACATCA GCACCATCAA GTCCCTCAGA GTTCTGCGAG TCTTACGGCC CCTCAAGACC 
ATCAAGCGCC TGCCAAAGCT GAAGGCTGTG TTTGACTGTG TGGTCAACTC CCTGAAGAAC 
GTCTTCAACA TCCTCATTGT CTACATGCTC TTCATGTTCA TCTTCGCTGT CATCGCTGTG 
CAGCTGTTCA AGGGAAAGTT CTTTTACTGC TCCGATGAGT CCAAGGGACT GGAAAAGGAC 
TGCAGAGGTC ATTTCCTGGA CTACGATAAG GATGAGGTGG CCGCCCAGCC TCGGGTGTGG 
AAGAAATATG AGTTCCACTA TGACAACGTG CTGTGGGCCT TCCTCACTCT CTTCACTGTG 
TCCACTGGAG AGGGCTGGCC CGATGTTCTG AAGCACTCTG TGGATGCTAC CTTTGAGGAC 
CAGGGTCCCA GTCCAGGCTA CCGTATAGAG ATCTCCATCT TCTACGTGGT CTACTTCGTG 
GTCTTTCCCT TCTTCTTCGT CAACATCTTT GTGGCCTTGA TCATCATTAC CTTCCAGGAG 
CAAGGGGACA AGGCCATGTC AGAGTGCAGC CTGGAGAAAA ACGAGAGGGC CTGCATTGAC 
TTTGCCATCA ACGCCAAGCC ACTGACGCGT TACATGCCAG AGAACGTGCA GTCCTTCCAG 
TACCGGATGT GGAAGTTTGT GGTGTCACCC CCGTTTGAGT ACGCCATTAT GATCATGATC 
GCCCTGAACA CCGTGGTGCT CATGATGAAG TTCCATGGAG CTCCAGACTT CTACGAGGCC 
ATGTTGAAGC ACTTCAACAT CGTCTTCACT GCCCTCTTCT CTCTGGAGTG CATTCTGAAG 
ATCATCGCCT TCGGACCACT GAACTACCTG AAGGATGCCT GGAACGTGTT TGACTTTGTG 
ACCGTGCTGG GAAGTATAAC TGACATCGTG GTCACAGAAA TCAATACTAC GGACCGGCTT 
TTGAACCTGA GTTTTCTGAG GCTGTTCAGA GCAGCTCGTC TCATCAAACT ACTGAGGCAA 
GGCTACACCA TCCGTATCCT GCTGTGGACC TTCGTCCAGT CCTTTAAGGC CCTGCCCTAT 
GTTTGCCTTC TGATAGCAAT GCTGTTCTTC ATCTATGCCA TCATCGGTAT GCAGGTGTTT 
GGAAACATTG AGCTCAACGA GGACACATCC ATCACTCACC ACAACAACTT CCGCACTTTT 
CTTCAGGCTC TCATGCTACT GTTCAGGAGT GCCACAGGGG AGGCGTGGCA TGAGATCATG 
CTGTCGTGTT TGAGTCACAG AGCCTGTGAT GAGAGGTCAG GCAACCTGGG GAAGGAGTGT 
GGCAGCGACT TTGCCTATTT CTACTTTGTG TCCTTCATCT TCCTCTGCTC TTTCCTGATG 
CTTAATCTGT TTGTGGCTGT GATCATGGAT AACTTTGAAT ACCTGACTCG AGACTCTTCT 
ATACTGGGGC CTCATCATCT GGATGAGTTC ATCCGTGTTT GGGCTGAGTA CGACCCTGCT 
GCCTGTGGCC GTATCAAGTA CCTCGATATG TATCAGATAC TCCTCCACAT GTCCCCACCC 
TTAGGTTTGG GAAAGAAATG TCCGCCAAGA GTCGCCTACA AGCGGCTAGT CAAGATGAAC 
ATGCCCATCG CTGATGACAA CACAGTCCAC TTCACCTCCA CACTCATGGC CCTGATCCGG 
ACGGCCCTGG AGATCAAACT GGCCTCAGGG ATGGTTGCCC AGCGTCTCTC TGATGCTGAA 
CTGAAGAAAG AGCTCTCCCA GGTTTGGCCA AGCCTCTCAC AGAAGACTAT GGATCTGTTG 
GTGACACCAC ACAAACCCAA TGAGCTTACT GTTGGGAAGG TGTATGCAGC CCTGATGATC 
TTTGACTACT ACAAGCAGAA CCGAGCCAAG AGGCTCAAGG AACAGCAACA GCAGCAGCAG 
CAACAGAATG CTACTGGCTC CCAGCAGGAG AAAGTGAAGG AGAAGGCCAT GACCAACGTG 
GAGCCTCCCT GTGAGCTACT GCCTCAGCCC CAGTCTCAGG CCCTACCTAG ACCCAGCTCC 
ACCCTGCTCA ACAACGGAGG CACACTGCCA GGCCATGACG GCACCATGAA AGGATCATCC 
TCCTGGGTGT CAGAGAAGTC TAGAGAGGTC CACAGACCCA GGATTAAGAC ACCTGTCCAA 
AGGGGCCAGT CTGAGGATGT CCCAAACCTA ACCATCCCCC AGGAGTCAGT GGAGATGAGG 
AAGATGGACA CCAGTTGCAG CACAGTGCCT GGTCCTGGGT CTGGACTGGA GAGCCAGGGA 
AGAGCTGTGT CCATGCCCCG CCTCAATGCA GAGCTGCAGA GGAGCAAGTC GCGCCACTCC 
CCGGGGACCC TGCTGGCACC TGTTCCTGAC TGCAGCCCAA TGAAACGCTC TGCGTCCACC 
CACGCCCCCC AGCGCCCCCA GGAGGTGAAC CTGCATGAAC ACACCCTGGA GAGGCCCAGC 
ACCGAAGAAA GGCGTCACCA TCACCGCCAC CACCACCGCT GCCACCACCA CCGAGAGAGG 
GACAAGAAGC AGCGGTCACT GGACAGGACG CCAAGCACCA TTGGTGCACC AGGTGACCCG 
GCTGCAGAGC CCTCGTCTAG AGACAGGGTG CAGGACCGCG GCCGCTCCCA TGAGAGGAAG 
AACCACTCGA CGGCGGGGGA GAAGCAGCGC TACTACTCTT GCGATCGCTA CAGCAGCAGG 
GAACACTGCC ACACCACCAA ATCTGCCACC ACTAGCTGTG CCACCTCCCC CAGCGAGGTG 
CAGGAACCCA GCATCAACAA GCAGGGTAGT GGTTCGGTTA AAGGCAGCCC AGTGATGTTG 
ACCTCAGGGG CTATCACACC TAGCCGTGGA CGCAGGCAGC TCCCACAGAC CCCCCTGACC 
CCCCGCCCCG GGGTGACGTT CAAGACTGCA AACTCCTCCC CTGTGCACTT TGTGTCTGCC 
CAGTGTGGCA GCGGCCTGCC CACCCTGAGT CCTGGCCGGC TGAGCCGTGG GCTGTCTGAG 
CACAACGCCC TGCTGCATAG TGGCACCTCT CACATCCCCT CCCCCATCAC CCGCATCAGC 
TCTGAGCCCT TCCTGGGCTA CAGTCACAGT GACCCCCAAG GCCAGGGTGG GCCGTACTGC 
AACCTCCGGG ACGAGCTGCT GAGCCCTTAT GGTGCCAGAG CACACTCCCC TCGGACTGCA 
CGTCCTCAAA TGAGGCCGGG GGCCCTCCAC CTGCCTCCGC CACCCCAGCA GAGACGTGCG 
GTGCCCAACG GATACCACTT CACCTTTGGT GGTAATGGCA ACGCCAGCCT GGGCTCTGGC 
TCTGGTGCCA GGCCACCCAG GTACTATCAC GAGGCAGAGG AGGATGAATG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014027086.1
CDS	635..8011
Translation	MARFEEELSNRRGGVCQAGPPRVAGRPAGHPSGPRVYKQSMAQRARTMAIYNPIPVKQSCLTVNRSLFIFSEDCIIRKYAKKITEWPPFEYLILATIIANCIVLALEQHLPASDKTPMSERLDDTEPYFIAIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGMLATVGADFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGMLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQAAEFPCGMEAPARTCENGTVCQEYWIGPNYGITNFDNILFAILTVFQCITMEGWVDILYNANDASGNTWNWIYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKHAEEKSPLDGAWYKRKHNNPVLKRAKKSKNDLINAEEGEDHFTDVSSVGSPFTRATPPGSPFTRAAPPGSPFTRAAPPGSPFTRATPPGSPFTRATPPGSPFTRATPPGSPFTRASLKSSKNDSASYFRRKEKRLRFTIRRLVKAQSFYWTVLCLVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLTEMSMKMYGLGPRNYFHSSFNCFDFGVIIGSIFEVIWAVVKPGASYGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGMFNFEEETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESHGGVRWGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEVAITKKMALQMVKEVKEVSPMSAANISITALHSMLYTGQHGAERAMPASFVKQSRGALSRSSSVCSVNSLSYVCSPVHHYLSKEQQKSLKMMSVWEQRTTQLRRHNLRASSEALYSELDPEERLRMSSALHIRPDMKTHLDRPLVVKPHDGPGPMGHLKPQTPEDTDNPGDPAGPQPRRHHRDRERPIDEANENGDPGKEGRHHVHHSRSKYPNGTRGKEGKSERSHSREGGRRHHHQTSVDEGGGGGTGTGERVHRHHHSHRQAREGNSTVNSGRKDERRSRHKDGRSGERCSRGENGANGERRRHKTHSYRGQSTLAGEEHSERERGKGHRDRYVESEGDSMATSTQQDPGDQRWGTEITEDSDNQRNVRRTGWTAVHIPPVTITSLPGETTLIPMNSIDCETLPMNEEKKTLEVLTRNGPRPILPYSSMFVFGQTNMVRRLCHYIVSLRYFEMCILVVISMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGPPQGVPRGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVIAVQLFKGKFFYCSDESKGLEKDCRGHFLDYDKDEVAAQPRVWKKYEFHYDNVLWAFLTLFTVSTGEGWPDVLKHSVDATFEDQGPSPGYRIEISIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPENVQSFQYRMWKFVVSPPFEYAIMIMIALNTVVLMMKFHGAPDFYEAMLKHFNIVFTALFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDIVVTEINTTDRLLNLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIELNEDTSITHHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSHRACDERSGNLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYQILLHMSPPLGLGKKCPPRVAYKRLVKMNMPIADDNTVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSQVWPSLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLKEQQQQQQQQNATGSQQEKVKEKAMTNVEPPCELLPQPQSQALPRPSSTLLNNGGTLPGHDGTMKGSSSWVSEKSREVHRPRIKTPVQRGQSEDVPNLTIPQESVEMRKMDTSCSTVPGPGSGLESQGRAVSMPRLNAELQRSKSRHSPGTLLAPVPDCSPMKRSASTHAPQRPQEVNLHEHTLERPSTEERRHHHRHHHRCHHHRERDKKQRSLDRTPSTIGAPGDPAAEPSSRDRVQDRGRSHERKNHSTAGEKQRYYSCDRYSSREHCHTTKSATTSCATSPSEVQEPSINKQGSGSVKGSPVMLTSGAITPSRGRRQLPQTPLTPRPGVTFKTANSSPVHFVSAQCGSGLPTLSPGRLSRGLSEHNALLHSGTSHIPSPITRISSEPFLGYSHSDPQGQGGPYCNLRDELLSPYGARAHSPRTARPQMRPGALHLPPPPQQRRAVPNGYHFTFGGNGNASLGSGSGARPPRYYHEAEEDEWC

Target ORF information:

RefSeq Version	XM_014171611.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014171611.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTTTTGT AAAGCAAAGT 
CGAGGTGCAC TCTCTCGCAG CTCGTCCGTC TGCAGCGTTA ATTCACTGAG TTATGTCTGC 
TCCCCAGTCC ATCACTACTT GAGTAAGGAG CAGCAGAAGT CTCTGAAGAT GATGTCAGTG 
TGGGAGCAGC GCACCACTCA GCTGAGGAGA CACAACCTGC GGGCCAGCAG TGAGGCCCTG 
TACAGCGAGC TGGACCCTGA GGAGAGGCTA CGCATGTCCT CCGCCCTGCA CATCCGCCCT 
GACATGAAGA CCCACCTGGA CCGGCCCCTG GTCGTGAAGC CCCACGACGG CCCCGGGCCC 
ATGGGCCACC TCAAGCCCCA GACCCCTGAG GACACGGATA ACCCTGGGGA CCCGGCCGGC 
CCCCAGCCAC GTAGGCACCA CCGGGACAGG GAGAGACCCA TCGACGAGGC CAATGAGAAT 
GGAGATCCAG GCAAAGAGGG TAGGCACCAT GTCCATCACA GTCGCAGCAA GTACCCCAAT 
GGGACTCGGG GTAAGGAGGG GAAGAGTGAG CGGTCGCATA GCCGAGAGGG TGGCAGGAGG 
CACCACCACC AGACCTCAGT GGACGAGGGA GGTGGAGGGG GAACTGGGAC GGGAGAGAGA 
GTGCACCGCC ACCACCACTC CCACAGACAG GCCAGAGAGG GTAACAGCAC TGTCAACAGC 
GGACGCAAGG ATGAGCGCAG GTCACGCCAC AAAGATGGAC GCTCGGGAGA GCGATGCTCC 
AGGGGAGAGA ACGGGGCCAA TGGGGAGAGG AGGAGACACA AGACCCACAG TTACAGGGGC 
CAGTCCACAC TGGCGGGAGA GGAGCACAGT GAGAGGGAGA GGGGGAAGGG CCACAGGGAC 
AGATATGTTG AGTCTGAGGG CGACTCCATG GCCACTAGCA CCCAGCAGGA TCCTGGAGAT 
CAGAGGTGGG GCACAGAGAT AACTGAGGAC TCAGACAACC AGAGGAACGT CAGACGGACA 
GGATGGACGG CCGTCCACAT ACCCCCTGTC ACCATCACCT CCCTGCCTGG AGAGACCACC 
CTTATCCCCA TGAACAGTAT AGACTGTGAG ACATTGCCTA TGAACGAGGA GAAGAAGACC 
CTGGAGGTGT TGACTCGGAA CGGACCCAGA CCCATCCTCC CCTACAGCTC CATGTTTGTG 
TTTGGCCAGA CCAACATGGT GCGGCGTCTG TGTCATTACA TTGTGAGCCT GCGGTACTTT 
GAGATGTGTA TCCTGGTAGT GATATCTATG AGTAGCATTG CATTGGCTGC TGAGGACCCT 
GTCCAGGCCA ATGCTCCACG CAACAATGTG CTCAAGTATC TGGACTACGT CTTTACGGGA 
GTTTTCACAT TTGAGATGGT GATTAAGATG GTTGACCTGG GCCTTCTGCT CCATCCTGGT 
TCTTACTTCA GAGATCTCTG GAACATTCTG GACTTCATTG TGGTCAGTGG AGCACTGGTG 
GCATTTGCAT TTTCGAGCTT CATGGGACCG CCACAAGGTG TGCCCAGAGG GTCCAAAGGA 
AAGGACATCA GCACCATCAA GTCCCTCAGA GTTCTGCGAG TCTTACGGCC CCTCAAGACC 
ATCAAGCGCC TGCCAAAGCT GAAGGCTGTG TTTGACTGTG TGGTCAACTC CCTGAAGAAC 
GTCTTCAACA TCCTCATTGT CTACATGCTC TTCATGTTCA TCTTCGCTGT CATCGCTGTG 
CAGCTGTTCA AGGGAAAGTT CTTTTACTGC TCCGATGAGT CCAAGGGACT GGAAAAGGAC 
TGCAGAGGTC ATTTCCTGGA CTACGATAAG GATGAGGTGG CCGCCCAGCC TCGGGTGTGG 
AAGAAATATG AGTTCCACTA TGACAACGTG CTGTGGGCCT TCCTCACTCT CTTCACTGTG 
TCCACTGGAG AGGGCTGGCC CGATGTTCTG AAGCACTCTG TGGATGCTAC CTTTGAGGAC 
CAGGGTCCCA GTCCAGGCTA CCGTATAGAG ATCTCCATCT TCTACGTGGT CTACTTCGTG 
GTCTTTCCCT TCTTCTTCGT CAACATCTTT GTGGCCTTGA TCATCATTAC CTTCCAGGAG 
CAAGGGGACA AGGCCATGTC AGAGTGCAGC CTGGAGAAAA ACGAGAGGGC CTGCATTGAC 
TTTGCCATCA ACGCCAAGCC ACTGACGCGT TACATGCCAG AGAACGTGCA GTCCTTCCAG 
TACCGGATGT GGAAGTTTGT GGTGTCACCC CCGTTTGAGT ACGCCATTAT GATCATGATC 
GCCCTGAACA CCGTGGTGCT CATGATGAAG TTCCATGGAG CTCCAGACTT CTACGAGGCC 
ATGTTGAAGC ACTTCAACAT CGTCTTCACT GCCCTCTTCT CTCTGGAGTG CATTCTGAAG 
ATCATCGCCT TCGGACCACT GAACTACCTG AAGGATGCCT GGAACGTGTT TGACTTTGTG 
ACCGTGCTGG GAAGTATAAC TGACATCGTG GTCACAGAAA TCAATACTAC GGACCGGCTT 
TTGAACCTGA GTTTTCTGAG GCTGTTCAGA GCAGCTCGTC TCATCAAACT ACTGAGGCAA 
GGCTACACCA TCCGTATCCT GCTGTGGACC TTCGTCCAGT CCTTTAAGGC CCTGCCCTAT 
GTTTGCCTTC TGATAGCAAT GCTGTTCTTC ATCTATGCCA TCATCGGTAT GCAGGTGTTT 
GGAAACATTG AGCTCAACGA GGACACATCC ATCACTCACC ACAACAACTT CCGCACTTTT 
CTTCAGGCTC TCATGCTACT GTTCAGGAGT GCCACAGGGG AGGCGTGGCA TGAGATCATG 
CTGTCGTGTT TGAGTCACAG AGCCTGTGAT GAGAGGTCAG GCAACCTGGG GAAGGAGTGT 
GGCAGCGACT TTGCCTATTT CTACTTTGTG TCCTTCATCT TCCTCTGCTC TTTCCTGATG 
CTTAATCTGT TTGTGGCTGT GATCATGGAT AACTTTGAAT ACCTGACTCG AGACTCTTCT 
ATACTGGGGC CTCATCATCT GGATGAGTTC ATCCGTGTTT GGGCTGAGTA CGACCCTGCT 
GCCTGTGGCC GTATCAAGTA CCTCGATATG TATCAGATAC TCCTCCACAT GTCCCCACCC 
TTAGGTTTGG GAAAGAAATG TCCGCCAAGA GTCGCCTACA AGCGGCTAGT CAAGATGAAC 
ATGCCCATCG CTGATGACAA CACAGTCCAC TTCACCTCCA CACTCATGGC CCTGATCCGG 
ACGGCCCTGG AGATCAAACT GGCCTCAGGG ATGGTTGCCC AGCGTCTCTC TGATGCTGAA 
CTGAAGAAAG AGCTCTCCCA GGTTTGGCCA AGCCTCTCAC AGAAGACTAT GGATCTGTTG 
GTGACACCAC ACAAACCCAA TGAGCTTACT GTTGGGAAGG TGTATGCAGC CCTGATGATC 
TTTGACTACT ACAAGCAGAA CCGAGCCAAG AGGCTCAAGG AACAGCAACA GCAGCAGCAG 
CAACAGAATG CTACTGGCTC CCAGCAGGAG AAAGTGAAGG AGAAGGCCAT GACCAACGTG 
GAGCCTCCCT GTGAGCTACT GCCTCAGCCC CAGTCTCAGG CCCTACCTAG ACCCAGCTCC 
ACCCTGCTCA ACAACGGAGG CACACTGCCA GGCCATGACG GCACCATGAA AGGATCATCC 
TCCTGGGTGT CAGAGAAGTC TAGAGAGGTC CACAGACCCA GGATTAAGAC ACCTGTCCAA 
AGGGGCCAGT CTGAGGATGT CCCAAACCTA ACCATCCCCC AGGAGTCAGT GGAGATGAGG 
AAGATGGACA CCAGTTGCAG CACAGTGCCT GGTCCTGGGT CTGGACTGGA GAGCCAGGGA 
AGAGCTGTGT CCATGCCCCG CCTCAATGCA GAGCTGCAGA GGAGCAAGTC GCGCCACTCC 
CCGGGGACCC TGCTGGCACC TGTTCCTGAC TGCAGCCCAA TGAAACGCTC TGCGTCCACC 
CACGCCCCCC AGCGCCCCCA GGAGGTGAAC CTGCATGAAC ACACCCTGGA GAGGCCCAGC 
ACCGAAGAAA GGCGTCACCA TCACCGCCAC CACCACCGCT GCCACCACCA CCGAGAGAGG 
GACAAGAAGC AGCGGTCACT GGACAGGACG CCAAGCACCA TTGGTGCACC AGGTGACCCG 
GCTGCAGAGC CCTCGTCTAG AGACAGGGTG CAGGACCGCG GCCGCTCCCA TGAGAGGAAG 
AACCACTCGA CGGCGGGGGA GAAGCAGCGC TACTACTCTT GCGATCGCTA CAGCAGCAGG 
GAACACTGCC ACACCACCAA ATCTGCCACC ACTAGCTGTG CCACCTCCCC CAGCGAGGTG 
CAGGAACCCA GCATCAACAA GCAGGGTAGT GGTTCGGTTA AAGGCAGCCC AGTGATGTTG 
ACCTCAGGGG CTATCACACC TAGCCGTGGA CGCAGGCAGC TCCCACAGAC CCCCCTGACC 
CCCCGCCCCG GGGTGACGTT CAAGACTGCA AACTCCTCCC CTGTGCACTT TGTGTCTGCC 
CAGTGTGGCA GCGGCCTGCC CACCCTGAGT CCTGGCCGGC TGAGCCGTGG GCTGTCTGAG 
CACAACGCCC TGCTGCATAG TGGCACCTCT CACATCCCCT CCCCCATCAC CCGCATCAGC 
TCTGAGCCCT TCCTGGGCTA CAGTCACAGT GACCCCCAAG GCCAGGGTGG GCCGTACTGC 
AACCTCCGGG ACGAGCTGCT GAGCCCTTAT GGTGCCAGAG CACACTCCCC TCGGACTGCA 
CGTCCTCAAA TGAGGCCGGG GGCCCTCCAC CTGCCTCCGC CACCCCAGCA GAGACGTGCG 
GTGCCCAACG GATACCACTT CACCTTTGGT GGTAATGGCA ACGCCAGCCT GGGCTCTGGC 
TCTGGTGCCA GGCCACCCAG GTACTATCAC GAGGCAGAGG AGGATGAATG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106585428 ( XM_014171612.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014027087.1 Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X6, mRNA.

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CloneID	OAt67169
Clone ID Related Accession (Same CDS sequence)	XM_014171612.1
Accession Version	XM_014171612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012340658.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTAAGGA GCAGCAGAAG 
TCTCTGAAGA TGATGTCAGT GTGGGAGCAG CGCACCACTC AGCTGAGGAG ACACAACCTG 
CGGGCCAGCA GTGAGGCCCT GTACAGCGAG CTGGACCCTG AGGAGAGGCT ACGCATGTCC 
TCCGCCCTGC ACATCCGCCC TGACATGAAG ACCCACCTGG ACCGGCCCCT GGTCGTGAAG 
CCCCACGACG GCCCCGGGCC CATGGGCCAC CTCAAGCCCC AGACCCCTGA GGACACGGAT 
AACCCTGGGG ACCCGGCCGG CCCCCAGCCA CGTAGGCACC ACCGGGACAG GGAGAGACCC 
ATCGACGAGG CCAATGAGAA TGGAGATCCA GGCAAAGAGG GTAGGCACCA TGTCCATCAC 
AGTCGCAGCA AGTACCCCAA TGGGACTCGG GGTAAGGAGG GGAAGAGTGA GCGGTCGCAT 
AGCCGAGAGG GTGGCAGGAG GCACCACCAC CAGACCTCAG TGGACGAGGG AGGTGGAGGG 
GGAACTGGGA CGGGAGAGAG AGTGCACCGC CACCACCACT CCCACAGACA GGCCAGAGAG 
GGTAACAGCA CTGTCAACAG CGGACGCAAG GATGAGCGCA GGTCACGCCA CAAAGATGGA 
CGCTCGGGAG AGCGATGCTC CAGGGGAGAG AACGGGGCCA ATGGGGAGAG GAGGAGACAC 
AAGACCCACA GTTACAGGGG CCAGTCCACA CTGGCGGGAG AGGAGCACAG TGAGAGGGAG 
AGGGGGAAGG GCCACAGGGA CAGATATGTT GAGTCTGAGG GCGACTCCAT GGCCACTAGC 
ACCCAGCAGG ATCCTGGAGA TCAGAGGTGG GGCACAGAGA TAACTGAGGA CTCAGACAAC 
CAGAGGAACG TCAGACGGAC AGGATGGACG GCCGTCCACA TACCCCCTGT CACCATCACC 
TCCCTGCCTG GAGAGACCAC CCTTATCCCC ATGAACAGTA TAGACTGTGA GACATTGCCT 
ATGAACGAGG AGAAGAAGAC CCTGGAGGTG TTGACTCGGA ACGGACCCAG ACCCATCCTC 
CCCTACAGCT CCATGTTTGT GTTTGGCCAG ACCAACATGG TGCGGCGTCT GTGTCATTAC 
ATTGTGAGCC TGCGGTACTT TGAGATGTGT ATCCTGGTAG TGATATCTAT GAGTAGCATT 
GCATTGGCTG CTGAGGACCC TGTCCAGGCC AATGCTCCAC GCAACAATGT GCTCAAGTAT 
CTGGACTACG TCTTTACGGG AGTTTTCACA TTTGAGATGG TGATTAAGAT GGTTGACCTG 
GGCCTTCTGC TCCATCCTGG TTCTTACTTC AGAGATCTCT GGAACATTCT GGACTTCATT 
GTGGTCAGTG GAGCACTGGT GGCATTTGCA TTTTCGAGCT TCATGGGACC GCCACAAGGT 
GTGCCCAGAG GGTCCAAAGG AAAGGACATC AGCACCATCA AGTCCCTCAG AGTTCTGCGA 
GTCTTACGGC CCCTCAAGAC CATCAAGCGC CTGCCAAAGC TGAAGGCTGT GTTTGACTGT 
GTGGTCAACT CCCTGAAGAA CGTCTTCAAC ATCCTCATTG TCTACATGCT CTTCATGTTC 
ATCTTCGCTG TCATCGCTGT GCAGCTGTTC AAGGGAAAGT TCTTTTACTG CTCCGATGAG 
TCCAAGGGAC TGGAAAAGGA CTGCAGAGGT CATTTCCTGG ACTACGATAA GGATGAGGTG 
GCCGCCCAGC CTCGGGTGTG GAAGAAATAT GAGTTCCACT ATGACAACGT GCTGTGGGCC 
TTCCTCACTC TCTTCACTGT GTCCACTGGA GAGGGCTGGC CCGATGTTCT GAAGCACTCT 
GTGGATGCTA CCTTTGAGGA CCAGGGTCCC AGTCCAGGCT ACCGTATAGA GATCTCCATC 
TTCTACGTGG TCTACTTCGT GGTCTTTCCC TTCTTCTTCG TCAACATCTT TGTGGCCTTG 
ATCATCATTA CCTTCCAGGA GCAAGGGGAC AAGGCCATGT CAGAGTGCAG CCTGGAGAAA 
AACGAGAGGG CCTGCATTGA CTTTGCCATC AACGCCAAGC CACTGACGCG TTACATGCCA 
GAGAACGTGC AGTCCTTCCA GTACCGGATG TGGAAGTTTG TGGTGTCACC CCCGTTTGAG 
TACGCCATTA TGATCATGAT CGCCCTGAAC ACCGTGGTGC TCATGATGAA GTTCCATGGA 
GCTCCAGACT TCTACGAGGC CATGTTGAAG CACTTCAACA TCGTCTTCAC TGCCCTCTTC 
TCTCTGGAGT GCATTCTGAA GATCATCGCC TTCGGACCAC TGAACTACCT GAAGGATGCC 
TGGAACGTGT TTGACTTTGT GACCGTGCTG GGAAGTATAA CTGACATCGT GGTCACAGAA 
ATCAATACTA CGGACCGGCT TTTGAACCTG AGTTTTCTGA GGCTGTTCAG AGCAGCTCGT 
CTCATCAAAC TACTGAGGCA AGGCTACACC ATCCGTATCC TGCTGTGGAC CTTCGTCCAG 
TCCTTTAAGG CCCTGCCCTA TGTTTGCCTT CTGATAGCAA TGCTGTTCTT CATCTATGCC 
ATCATCGGTA TGCAGGTGTT TGGAAACATT GAGCTCAACG AGGACACATC CATCACTCAC 
CACAACAACT TCCGCACTTT TCTTCAGGCT CTCATGCTAC TGTTCAGGAG TGCCACAGGG 
GAGGCGTGGC ATGAGATCAT GCTGTCGTGT TTGAGTCACA GAGCCTGTGA TGAGAGGTCA 
GGCAACCTGG GGAAGGAGTG TGGCAGCGAC TTTGCCTATT TCTACTTTGT GTCCTTCATC 
TTCCTCTGCT CTTTCCTGAT GCTTAATCTG TTTGTGGCTG TGATCATGGA TAACTTTGAA 
TACCTGACTC GAGACTCTTC TATACTGGGG CCTCATCATC TGGATGAGTT CATCCGTGTT 
TGGGCTGAGT ACGACCCTGC TGCCTGTGGC CGTATCAAGT ACCTCGATAT GTATCAGATA 
CTCCTCCACA TGTCCCCACC CTTAGGTTTG GGAAAGAAAT GTCCGCCAAG AGTCGCCTAC 
AAGCGGCTAG TCAAGATGAA CATGCCCATC GCTGATGACA ACACAGTCCA CTTCACCTCC 
ACACTCATGG CCCTGATCCG GACGGCCCTG GAGATCAAAC TGGCCTCAGG GATGGTTGCC 
CAGCGTCTCT CTGATGCTGA ACTGAAGAAA GAGCTCTCCC AGGTTTGGCC AAGCCTCTCA 
CAGAAGACTA TGGATCTGTT GGTGACACCA CACAAACCCA ATGAGCTTAC TGTTGGGAAG 
GTGTATGCAG CCCTGATGAT CTTTGACTAC TACAAGCAGA ACCGAGCCAA GAGGCTCAAG 
GAACAGCAAC AGCAGCAGCA GCAACAGAAT GCTACTGGCT CCCAGGGAAA GGTGGGGGCT 
CTGTTTAAAC CAATACTGCC CCTGACTCAC AAGCAGGAGA AAGTGAAGGA GAAGGCCATG 
ACCAACGTGG AGCCTCCCTG TGAGCTACTG CCTCAGCCCC AGTCTCAGGC CCTACCTAGA 
CCCAGCTCCA CCCTGCTCAA CAACGGAGGC ACACTGCCAG GCCATGACGG CACCATGAAA 
GGATCATCCT CCTGGGTGTC AGAGAAGTCT AGAGAGGTCC ACAGACCCAG GATTAAGACA 
CCTGTCCAAA GGGGCCAGTC TGAGGATGTC CCAAACCTAA CCATCCCCCA GGAGTCAGTG 
GAGATGAGGA AGATGGACAC CAGTTGCAGC ACAGTGCCTG GTCCTGGGTC TGGACTGGAG 
AGCCAGGGAA GAGCTGTGTC CATGCCCCGC CTCAATGCAG AGCTGCAGAG GAGCAAGTCG 
CGCCACTCCC CGGGGACCCT GCTGGCACCT GTTCCTGACT GCAGCCCAAT GAAACGCTCT 
GCGTCCACCC ACGCCCCCCA GCGCCCCCAG GAGGTGAACC TGCATGAACA CACCCTGGAG 
AGGCCCAGCA CCGAAGAAAG GCGTCACCAT CACCGCCACC ACCACCGCTG CCACCACCAC 
CGAGAGAGGG ACAAGAAGCA GCGGTCACTG GACAGGACGC CAAGCACCAT TGGTGCACCA 
GGTGACCCGG CTGCAGAGCC CTCGTCTAGA GACAGGGTGC AGGACCGCGG CCGCTCCCAT 
GAGAGGAAGA ACCACTCGAC GGCGGGGGAG AAGCAGCGCT ACTACTCTTG CGATCGCTAC 
AGCAGCAGGG AACACTGCCA CACCACCAAA TCTGCCACCA CTAGCTGTGC CACCTCCCCC 
AGCGAGGTGC AGGAACCCAG CATCAACAAG CAGGGTAGTG GTTCGGTTAA AGGCAGCCCA 
GTGATGTTGA CCTCAGGGGC TATCACACCT AGCCGTGGAC GCAGGCAGCT CCCACAGACC 
CCCCTGACCC CCCGCCCCGG GGTGACGTTC AAGACTGCAA ACTCCTCCCC TGTGCACTTT 
GTGTCTGCCC AGTGTGGCAG CGGCCTGCCC ACCCTGAGTC CTGGCCGGCT GAGCCGTGGG 
CTGTCTGAGC ACAACGCCCT GCTGCATAGT GGCACCTCTC ACATCCCCTC CCCCATCACC 
CGCATCAGCT CTGAGCCCTT CCTGGGCTAC AGTCACAGTG ACCCCCAAGG CCAGGGTGGG 
CCGTACTGCA ACCTCCGGGA CGAGCTGCTG AGCCCTTATG GTGCCAGAGC ACACTCCCCT 
CGGACTGCAC GTCCTCAAAT GAGGCCGGGG GCCCTCCACC TGCCTCCGCC ACCCCAGCAG 
AGACGTGCGG TGCCCAACGG ATACCACTTC ACCTTTGGTG GTAATGGCAA CGCCAGCCTG 
GGCTCTGGCT CTGGTGCCAG GCCACCCAGG TACTATCACG AGGCAGAGGA GGATGAATGG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014027087.1
CDS	635..7960
Translation	MARFEEELSNRRGGVCQAGPPRVAGRPAGHPSGPRVYKQSMAQRARTMAIYNPIPVKQSCLTVNRSLFIFSEDCIIRKYAKKITEWPPFEYLILATIIANCIVLALEQHLPASDKTPMSERLDDTEPYFIAIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGMLATVGADFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGMLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQAAEFPCGMEAPARTCENGTVCQEYWIGPNYGITNFDNILFAILTVFQCITMEGWVDILYNANDASGNTWNWIYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKHAEEKSPLDGAWYKRKHNNPVLKRAKKSKNDLINAEEGEDHFTDVSSVGSPFTRATPPGSPFTRAAPPGSPFTRAAPPGSPFTRATPPGSPFTRATPPGSPFTRATPPGSPFTRASLKSSKNDSASYFRRKEKRLRFTIRRLVKAQSFYWTVLCLVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLTEMSMKMYGLGPRNYFHSSFNCFDFGVIIGSIFEVIWAVVKPGASYGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGMFNFEEETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESHGGVRWGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEVAITKKMALQMVKEVKEVSPMSAANISITALHSMLYTGQHGAERAMPASKEQQKSLKMMSVWEQRTTQLRRHNLRASSEALYSELDPEERLRMSSALHIRPDMKTHLDRPLVVKPHDGPGPMGHLKPQTPEDTDNPGDPAGPQPRRHHRDRERPIDEANENGDPGKEGRHHVHHSRSKYPNGTRGKEGKSERSHSREGGRRHHHQTSVDEGGGGGTGTGERVHRHHHSHRQAREGNSTVNSGRKDERRSRHKDGRSGERCSRGENGANGERRRHKTHSYRGQSTLAGEEHSERERGKGHRDRYVESEGDSMATSTQQDPGDQRWGTEITEDSDNQRNVRRTGWTAVHIPPVTITSLPGETTLIPMNSIDCETLPMNEEKKTLEVLTRNGPRPILPYSSMFVFGQTNMVRRLCHYIVSLRYFEMCILVVISMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGPPQGVPRGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVIAVQLFKGKFFYCSDESKGLEKDCRGHFLDYDKDEVAAQPRVWKKYEFHYDNVLWAFLTLFTVSTGEGWPDVLKHSVDATFEDQGPSPGYRIEISIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPENVQSFQYRMWKFVVSPPFEYAIMIMIALNTVVLMMKFHGAPDFYEAMLKHFNIVFTALFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDIVVTEINTTDRLLNLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIELNEDTSITHHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSHRACDERSGNLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYQILLHMSPPLGLGKKCPPRVAYKRLVKMNMPIADDNTVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSQVWPSLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLKEQQQQQQQQNATGSQGKVGALFKPILPLTHKQEKVKEKAMTNVEPPCELLPQPQSQALPRPSSTLLNNGGTLPGHDGTMKGSSSWVSEKSREVHRPRIKTPVQRGQSEDVPNLTIPQESVEMRKMDTSCSTVPGPGSGLESQGRAVSMPRLNAELQRSKSRHSPGTLLAPVPDCSPMKRSASTHAPQRPQEVNLHEHTLERPSTEERRHHHRHHHRCHHHRERDKKQRSLDRTPSTIGAPGDPAAEPSSRDRVQDRGRSHERKNHSTAGEKQRYYSCDRYSSREHCHTTKSATTSCATSPSEVQEPSINKQGSGSVKGSPVMLTSGAITPSRGRRQLPQTPLTPRPGVTFKTANSSPVHFVSAQCGSGLPTLSPGRLSRGLSEHNALLHSGTSHIPSPITRISSEPFLGYSHSDPQGQGGPYCNLRDELLSPYGARAHSPRTARPQMRPGALHLPPPPQQRRAVPNGYHFTFGGNGNASLGSGSGARPPRYYHEAEEDEWC

Target ORF information:

RefSeq Version	XM_014171612.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014171612.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCGCT ACACAGTATG 
CTGTACACAG GCCAACATGG AGCGGAGAGG GCAATGCCAG CAAGTAAGGA GCAGCAGAAG 
TCTCTGAAGA TGATGTCAGT GTGGGAGCAG CGCACCACTC AGCTGAGGAG ACACAACCTG 
CGGGCCAGCA GTGAGGCCCT GTACAGCGAG CTGGACCCTG AGGAGAGGCT ACGCATGTCC 
TCCGCCCTGC ACATCCGCCC TGACATGAAG ACCCACCTGG ACCGGCCCCT GGTCGTGAAG 
CCCCACGACG GCCCCGGGCC CATGGGCCAC CTCAAGCCCC AGACCCCTGA GGACACGGAT 
AACCCTGGGG ACCCGGCCGG CCCCCAGCCA CGTAGGCACC ACCGGGACAG GGAGAGACCC 
ATCGACGAGG CCAATGAGAA TGGAGATCCA GGCAAAGAGG GTAGGCACCA TGTCCATCAC 
AGTCGCAGCA AGTACCCCAA TGGGACTCGG GGTAAGGAGG GGAAGAGTGA GCGGTCGCAT 
AGCCGAGAGG GTGGCAGGAG GCACCACCAC CAGACCTCAG TGGACGAGGG AGGTGGAGGG 
GGAACTGGGA CGGGAGAGAG AGTGCACCGC CACCACCACT CCCACAGACA GGCCAGAGAG 
GGTAACAGCA CTGTCAACAG CGGACGCAAG GATGAGCGCA GGTCACGCCA CAAAGATGGA 
CGCTCGGGAG AGCGATGCTC CAGGGGAGAG AACGGGGCCA ATGGGGAGAG GAGGAGACAC 
AAGACCCACA GTTACAGGGG CCAGTCCACA CTGGCGGGAG AGGAGCACAG TGAGAGGGAG 
AGGGGGAAGG GCCACAGGGA CAGATATGTT GAGTCTGAGG GCGACTCCAT GGCCACTAGC 
ACCCAGCAGG ATCCTGGAGA TCAGAGGTGG GGCACAGAGA TAACTGAGGA CTCAGACAAC 
CAGAGGAACG TCAGACGGAC AGGATGGACG GCCGTCCACA TACCCCCTGT CACCATCACC 
TCCCTGCCTG GAGAGACCAC CCTTATCCCC ATGAACAGTA TAGACTGTGA GACATTGCCT 
ATGAACGAGG AGAAGAAGAC CCTGGAGGTG TTGACTCGGA ACGGACCCAG ACCCATCCTC 
CCCTACAGCT CCATGTTTGT GTTTGGCCAG ACCAACATGG TGCGGCGTCT GTGTCATTAC 
ATTGTGAGCC TGCGGTACTT TGAGATGTGT ATCCTGGTAG TGATATCTAT GAGTAGCATT 
GCATTGGCTG CTGAGGACCC TGTCCAGGCC AATGCTCCAC GCAACAATGT GCTCAAGTAT 
CTGGACTACG TCTTTACGGG AGTTTTCACA TTTGAGATGG TGATTAAGAT GGTTGACCTG 
GGCCTTCTGC TCCATCCTGG TTCTTACTTC AGAGATCTCT GGAACATTCT GGACTTCATT 
GTGGTCAGTG GAGCACTGGT GGCATTTGCA TTTTCGAGCT TCATGGGACC GCCACAAGGT 
GTGCCCAGAG GGTCCAAAGG AAAGGACATC AGCACCATCA AGTCCCTCAG AGTTCTGCGA 
GTCTTACGGC CCCTCAAGAC CATCAAGCGC CTGCCAAAGC TGAAGGCTGT GTTTGACTGT 
GTGGTCAACT CCCTGAAGAA CGTCTTCAAC ATCCTCATTG TCTACATGCT CTTCATGTTC 
ATCTTCGCTG TCATCGCTGT GCAGCTGTTC AAGGGAAAGT TCTTTTACTG CTCCGATGAG 
TCCAAGGGAC TGGAAAAGGA CTGCAGAGGT CATTTCCTGG ACTACGATAA GGATGAGGTG 
GCCGCCCAGC CTCGGGTGTG GAAGAAATAT GAGTTCCACT ATGACAACGT GCTGTGGGCC 
TTCCTCACTC TCTTCACTGT GTCCACTGGA GAGGGCTGGC CCGATGTTCT GAAGCACTCT 
GTGGATGCTA CCTTTGAGGA CCAGGGTCCC AGTCCAGGCT ACCGTATAGA GATCTCCATC 
TTCTACGTGG TCTACTTCGT GGTCTTTCCC TTCTTCTTCG TCAACATCTT TGTGGCCTTG 
ATCATCATTA CCTTCCAGGA GCAAGGGGAC AAGGCCATGT CAGAGTGCAG CCTGGAGAAA 
AACGAGAGGG CCTGCATTGA CTTTGCCATC AACGCCAAGC CACTGACGCG TTACATGCCA 
GAGAACGTGC AGTCCTTCCA GTACCGGATG TGGAAGTTTG TGGTGTCACC CCCGTTTGAG 
TACGCCATTA TGATCATGAT CGCCCTGAAC ACCGTGGTGC TCATGATGAA GTTCCATGGA 
GCTCCAGACT TCTACGAGGC CATGTTGAAG CACTTCAACA TCGTCTTCAC TGCCCTCTTC 
TCTCTGGAGT GCATTCTGAA GATCATCGCC TTCGGACCAC TGAACTACCT GAAGGATGCC 
TGGAACGTGT TTGACTTTGT GACCGTGCTG GGAAGTATAA CTGACATCGT GGTCACAGAA 
ATCAATACTA CGGACCGGCT TTTGAACCTG AGTTTTCTGA GGCTGTTCAG AGCAGCTCGT 
CTCATCAAAC TACTGAGGCA AGGCTACACC ATCCGTATCC TGCTGTGGAC CTTCGTCCAG 
TCCTTTAAGG CCCTGCCCTA TGTTTGCCTT CTGATAGCAA TGCTGTTCTT CATCTATGCC 
ATCATCGGTA TGCAGGTGTT TGGAAACATT GAGCTCAACG AGGACACATC CATCACTCAC 
CACAACAACT TCCGCACTTT TCTTCAGGCT CTCATGCTAC TGTTCAGGAG TGCCACAGGG 
GAGGCGTGGC ATGAGATCAT GCTGTCGTGT TTGAGTCACA GAGCCTGTGA TGAGAGGTCA 
GGCAACCTGG GGAAGGAGTG TGGCAGCGAC TTTGCCTATT TCTACTTTGT GTCCTTCATC 
TTCCTCTGCT CTTTCCTGAT GCTTAATCTG TTTGTGGCTG TGATCATGGA TAACTTTGAA 
TACCTGACTC GAGACTCTTC TATACTGGGG CCTCATCATC TGGATGAGTT CATCCGTGTT 
TGGGCTGAGT ACGACCCTGC TGCCTGTGGC CGTATCAAGT ACCTCGATAT GTATCAGATA 
CTCCTCCACA TGTCCCCACC CTTAGGTTTG GGAAAGAAAT GTCCGCCAAG AGTCGCCTAC 
AAGCGGCTAG TCAAGATGAA CATGCCCATC GCTGATGACA ACACAGTCCA CTTCACCTCC 
ACACTCATGG CCCTGATCCG GACGGCCCTG GAGATCAAAC TGGCCTCAGG GATGGTTGCC 
CAGCGTCTCT CTGATGCTGA ACTGAAGAAA GAGCTCTCCC AGGTTTGGCC AAGCCTCTCA 
CAGAAGACTA TGGATCTGTT GGTGACACCA CACAAACCCA ATGAGCTTAC TGTTGGGAAG 
GTGTATGCAG CCCTGATGAT CTTTGACTAC TACAAGCAGA ACCGAGCCAA GAGGCTCAAG 
GAACAGCAAC AGCAGCAGCA GCAACAGAAT GCTACTGGCT CCCAGGGAAA GGTGGGGGCT 
CTGTTTAAAC CAATACTGCC CCTGACTCAC AAGCAGGAGA AAGTGAAGGA GAAGGCCATG 
ACCAACGTGG AGCCTCCCTG TGAGCTACTG CCTCAGCCCC AGTCTCAGGC CCTACCTAGA 
CCCAGCTCCA CCCTGCTCAA CAACGGAGGC ACACTGCCAG GCCATGACGG CACCATGAAA 
GGATCATCCT CCTGGGTGTC AGAGAAGTCT AGAGAGGTCC ACAGACCCAG GATTAAGACA 
CCTGTCCAAA GGGGCCAGTC TGAGGATGTC CCAAACCTAA CCATCCCCCA GGAGTCAGTG 
GAGATGAGGA AGATGGACAC CAGTTGCAGC ACAGTGCCTG GTCCTGGGTC TGGACTGGAG 
AGCCAGGGAA GAGCTGTGTC CATGCCCCGC CTCAATGCAG AGCTGCAGAG GAGCAAGTCG 
CGCCACTCCC CGGGGACCCT GCTGGCACCT GTTCCTGACT GCAGCCCAAT GAAACGCTCT 
GCGTCCACCC ACGCCCCCCA GCGCCCCCAG GAGGTGAACC TGCATGAACA CACCCTGGAG 
AGGCCCAGCA CCGAAGAAAG GCGTCACCAT CACCGCCACC ACCACCGCTG CCACCACCAC 
CGAGAGAGGG ACAAGAAGCA GCGGTCACTG GACAGGACGC CAAGCACCAT TGGTGCACCA 
GGTGACCCGG CTGCAGAGCC CTCGTCTAGA GACAGGGTGC AGGACCGCGG CCGCTCCCAT 
GAGAGGAAGA ACCACTCGAC GGCGGGGGAG AAGCAGCGCT ACTACTCTTG CGATCGCTAC 
AGCAGCAGGG AACACTGCCA CACCACCAAA TCTGCCACCA CTAGCTGTGC CACCTCCCCC 
AGCGAGGTGC AGGAACCCAG CATCAACAAG CAGGGTAGTG GTTCGGTTAA AGGCAGCCCA 
GTGATGTTGA CCTCAGGGGC TATCACACCT AGCCGTGGAC GCAGGCAGCT CCCACAGACC 
CCCCTGACCC CCCGCCCCGG GGTGACGTTC AAGACTGCAA ACTCCTCCCC TGTGCACTTT 
GTGTCTGCCC AGTGTGGCAG CGGCCTGCCC ACCCTGAGTC CTGGCCGGCT GAGCCGTGGG 
CTGTCTGAGC ACAACGCCCT GCTGCATAGT GGCACCTCTC ACATCCCCTC CCCCATCACC 
CGCATCAGCT CTGAGCCCTT CCTGGGCTAC AGTCACAGTG ACCCCCAAGG CCAGGGTGGG 
CCGTACTGCA ACCTCCGGGA CGAGCTGCTG AGCCCTTATG GTGCCAGAGC ACACTCCCCT 
CGGACTGCAC GTCCTCAAAT GAGGCCGGGG GCCCTCCACC TGCCTCCGCC ACCCCAGCAG 
AGACGTGCGG TGCCCAACGG ATACCACTTC ACCTTTGGTG GTAATGGCAA CGCCAGCCTG 
GGCTCTGGCT CTGGTGCCAG GCCACCCAGG TACTATCACG AGGCAGAGGA GGATGAATGG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106585428 ( XM_014171613.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014027088.1 Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X7, mRNA.

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CloneID	OAt67170
Clone ID Related Accession (Same CDS sequence)	XM_014171613.1
Accession Version	XM_014171613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012340658.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCTAA GGAGCAGCAG 
AAGTCTCTGA AGATGATGTC AGTGTGGGAG CAGCGCACCA CTCAGCTGAG GAGACACAAC 
CTGCGGGCCA GCAGTGAGGC CCTGTACAGC GAGCTGGACC CTGAGGAGAG GCTACGCATG 
TCCTCCGCCC TGCACATCCG CCCTGACATG AAGACCCACC TGGACCGGCC CCTGGTCGTG 
AAGCCCCACG ACGGCCCCGG GCCCATGGGC CACCTCAAGC CCCAGACCCC TGAGGACACG 
GATAACCCTG GGGACCCGGC CGGCCCCCAG CCACGTAGGC ACCACCGGGA CAGGGAGAGA 
CCCATCGACG AGGCCAATGA GAATGGAGAT CCAGGCAAAG AGGGTAGGCA CCATGTCCAT 
CACAGTCGCA GCAAGTACCC CAATGGGACT CGGGGTAAGG AGGGGAAGAG TGAGCGGTCG 
CATAGCCGAG AGGGTGGCAG GAGGCACCAC CACCAGACCT CAGTGGACGA GGGAGGTGGA 
GGGGGAACTG GGACGGGAGA GAGAGTGCAC CGCCACCACC ACTCCCACAG ACAGGCCAGA 
GAGGGTAACA GCACTGTCAA CAGCGGACGC AAGGATGAGC GCAGGTCACG CCACAAAGAT 
GGACGCTCGG GAGAGCGATG CTCCAGGGGA GAGAACGGGG CCAATGGGGA GAGGAGGAGA 
CACAAGACCC ACAGTTACAG GGGCCAGTCC ACACTGGCGG GAGAGGAGCA CAGTGAGAGG 
GAGAGGGGGA AGGGCCACAG GGACAGATAT GTTGAGTCTG AGGGCGACTC CATGGCCACT 
AGCACCCAGC AGGATCCTGG AGATCAGAGG TGGGGCACAG AGATAACTGA GGACTCAGAC 
AACCAGAGGA ACGTCAGACG GACAGGATGG ACGGCCGTCC ACATACCCCC TGTCACCATC 
ACCTCCCTGC CTGGAGAGAC CACCCTTATC CCCATGAACA GTATAGACTG TGAGACATTG 
CCTATGAACG AGGAGAAGAA GACCCTGGAG GTGTTGACTC GGAACGGACC CAGACCCATC 
CTCCCCTACA GCTCCATGTT TGTGTTTGGC CAGACCAACA TGGTGCGGCG TCTGTGTCAT 
TACATTGTGA GCCTGCGGTA CTTTGAGATG TGTATCCTGG TAGTGATATC TATGAGTAGC 
ATTGCATTGG CTGCTGAGGA CCCTGTCCAG GCCAATGCTC CACGCAACAA TGTGCTCAAG 
TATCTGGACT ACGTCTTTAC GGGAGTTTTC ACATTTGAGA TGGTGATTAA GATGGTTGAC 
CTGGGCCTTC TGCTCCATCC TGGTTCTTAC TTCAGAGATC TCTGGAACAT TCTGGACTTC 
ATTGTGGTCA GTGGAGCACT GGTGGCATTT GCATTTTCGA GCTTCATGGG ACCGCCACAA 
GGTGTGCCCA GAGGGTCCAA AGGAAAGGAC ATCAGCACCA TCAAGTCCCT CAGAGTTCTG 
CGAGTCTTAC GGCCCCTCAA GACCATCAAG CGCCTGCCAA AGCTGAAGGC TGTGTTTGAC 
TGTGTGGTCA ACTCCCTGAA GAACGTCTTC AACATCCTCA TTGTCTACAT GCTCTTCATG 
TTCATCTTCG CTGTCATCGC TGTGCAGCTG TTCAAGGGAA AGTTCTTTTA CTGCTCCGAT 
GAGTCCAAGG GACTGGAAAA GGACTGCAGA GGTCATTTCC TGGACTACGA TAAGGATGAG 
GTGGCCGCCC AGCCTCGGGT GTGGAAGAAA TATGAGTTCC ACTATGACAA CGTGCTGTGG 
GCCTTCCTCA CTCTCTTCAC TGTGTCCACT GGAGAGGGCT GGCCCGATGT TCTGAAGCAC 
TCTGTGGATG CTACCTTTGA GGACCAGGGT CCCAGTCCAG GCTACCGTAT AGAGATCTCC 
ATCTTCTACG TGGTCTACTT CGTGGTCTTT CCCTTCTTCT TCGTCAACAT CTTTGTGGCC 
TTGATCATCA TTACCTTCCA GGAGCAAGGG GACAAGGCCA TGTCAGAGTG CAGCCTGGAG 
AAAAACGAGA GGGCCTGCAT TGACTTTGCC ATCAACGCCA AGCCACTGAC GCGTTACATG 
CCAGAGAACG TGCAGTCCTT CCAGTACCGG ATGTGGAAGT TTGTGGTGTC ACCCCCGTTT 
GAGTACGCCA TTATGATCAT GATCGCCCTG AACACCGTGG TGCTCATGAT GAAGTTCCAT 
GGAGCTCCAG ACTTCTACGA GGCCATGTTG AAGCACTTCA ACATCGTCTT CACTGCCCTC 
TTCTCTCTGG AGTGCATTCT GAAGATCATC GCCTTCGGAC CACTGAACTA CCTGAAGGAT 
GCCTGGAACG TGTTTGACTT TGTGACCGTG CTGGGAAGTA TAACTGACAT CGTGGTCACA 
GAAATCAATA CTACGGACCG GCTTTTGAAC CTGAGTTTTC TGAGGCTGTT CAGAGCAGCT 
CGTCTCATCA AACTACTGAG GCAAGGCTAC ACCATCCGTA TCCTGCTGTG GACCTTCGTC 
CAGTCCTTTA AGGCCCTGCC CTATGTTTGC CTTCTGATAG CAATGCTGTT CTTCATCTAT 
GCCATCATCG GTATGCAGGT GTTTGGAAAC ATTGAGCTCA ACGAGGACAC ATCCATCACT 
CACCACAACA ACTTCCGCAC TTTTCTTCAG GCTCTCATGC TACTGTTCAG GAGTGCCACA 
GGGGAGGCGT GGCATGAGAT CATGCTGTCG TGTTTGAGTC ACAGAGCCTG TGATGAGAGG 
TCAGGCAACC TGGGGAAGGA GTGTGGCAGC GACTTTGCCT ATTTCTACTT TGTGTCCTTC 
ATCTTCCTCT GCTCTTTCCT GATGCTTAAT CTGTTTGTGG CTGTGATCAT GGATAACTTT 
GAATACCTGA CTCGAGACTC TTCTATACTG GGGCCTCATC ATCTGGATGA GTTCATCCGT 
GTTTGGGCTG AGTACGACCC TGCTGCCTGT GGCCGTATCA AGTACCTCGA TATGTATCAG 
ATACTCCTCC ACATGTCCCC ACCCTTAGGT TTGGGAAAGA AATGTCCGCC AAGAGTCGCC 
TACAAGCGGC TAGTCAAGAT GAACATGCCC ATCGCTGATG ACAACACAGT CCACTTCACC 
TCCACACTCA TGGCCCTGAT CCGGACGGCC CTGGAGATCA AACTGGCCTC AGGGATGGTT 
GCCCAGCGTC TCTCTGATGC TGAACTGAAG AAAGAGCTCT CCCAGGTTTG GCCAAGCCTC 
TCACAGAAGA CTATGGATCT GTTGGTGACA CCACACAAAC CCAATGAGCT TACTGTTGGG 
AAGGTGTATG CAGCCCTGAT GATCTTTGAC TACTACAAGC AGAACCGAGC CAAGAGGCTC 
AAGGAACAGC AACAGCAGCA GCAGCAACAG AATGCTACTG GCTCCCAGGG AAAGGTGGGG 
GCTCTGTTTA AACCAATACT GCCCCTGACT CACAAGCAGG AGAAAGTGAA GGAGAAGGCC 
ATGACCAACG TGGAGCCTCC CTGTGAGCTA CTGCCTCAGC CCCAGTCTCA GGCCCTACCT 
AGACCCAGCT CCACCCTGCT CAACAACGGA GGCACACTGC CAGGCCATGA CGGCACCATG 
AAAGGATCAT CCTCCTGGGT GTCAGAGAAG TCTAGAGAGG TCCACAGACC CAGGATTAAG 
ACACCTGTCC AAAGGGGCCA GTCTGAGGAT GTCCCAAACC TAACCATCCC CCAGGAGTCA 
GTGGAGATGA GGAAGATGGA CACCAGTTGC AGCACAGTGC CTGGTCCTGG GTCTGGACTG 
GAGAGCCAGG GAAGAGCTGT GTCCATGCCC CGCCTCAATG CAGAGCTGCA GAGGAGCAAG 
TCGCGCCACT CCCCGGGGAC CCTGCTGGCA CCTGTTCCTG ACTGCAGCCC AATGAAACGC 
TCTGCGTCCA CCCACGCCCC CCAGCGCCCC CAGGAGGTGA ACCTGCATGA ACACACCCTG 
GAGAGGCCCA GCACCGAAGA AAGGCGTCAC CATCACCGCC ACCACCACCG CTGCCACCAC 
CACCGAGAGA GGGACAAGAA GCAGCGGTCA CTGGACAGGA CGCCAAGCAC CATTGGTGCA 
CCAGGTGACC CGGCTGCAGA GCCCTCGTCT AGAGACAGGG TGCAGGACCG CGGCCGCTCC 
CATGAGAGGA AGAACCACTC GACGGCGGGG GAGAAGCAGC GCTACTACTC TTGCGATCGC 
TACAGCAGCA GGGAACACTG CCACACCACC AAATCTGCCA CCACTAGCTG TGCCACCTCC 
CCCAGCGAGG TGCAGGAACC CAGCATCAAC AAGCAGGGTA GTGGTTCGGT TAAAGGCAGC 
CCAGTGATGT TGACCTCAGG GGCTATCACA CCTAGCCGTG GACGCAGGCA GCTCCCACAG 
ACCCCCCTGA CCCCCCGCCC CGGGGTGACG TTCAAGACTG CAAACTCCTC CCCTGTGCAC 
TTTGTGTCTG CCCAGTGTGG CAGCGGCCTG CCCACCCTGA GTCCTGGCCG GCTGAGCCGT 
GGGCTGTCTG AGCACAACGC CCTGCTGCAT AGTGGCACCT CTCACATCCC CTCCCCCATC 
ACCCGCATCA GCTCTGAGCC CTTCCTGGGC TACAGTCACA GTGACCCCCA AGGCCAGGGT 
GGGCCGTACT GCAACCTCCG GGACGAGCTG CTGAGCCCTT ATGGTGCCAG AGCACACTCC 
CCTCGGACTG CACGTCCTCA AATGAGGCCG GGGGCCCTCC ACCTGCCTCC GCCACCCCAG 
CAGAGACGTG CGGTGCCCAA CGGATACCAC TTCACCTTTG GTGGTAATGG CAACGCCAGC 
CTGGGCTCTG GCTCTGGTGC CAGGCCACCC AGGTACTATC ACGAGGCAGA GGAGGATGAA 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014027088.1
CDS	635..7903
Translation	MARFEEELSNRRGGVCQAGPPRVAGRPAGHPSGPRVYKQSMAQRARTMAIYNPIPVKQSCLTVNRSLFIFSEDCIIRKYAKKITEWPPFEYLILATIIANCIVLALEQHLPASDKTPMSERLDDTEPYFIAIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGMLATVGADFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGMLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQAAEFPCGMEAPARTCENGTVCQEYWIGPNYGITNFDNILFAILTVFQCITMEGWVDILYNANDASGNTWNWIYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKHAEEKSPLDGAWYKRKHNNPVLKRAKKSKNDLINAEEGEDHFTDVSSVGSPFTRATPPGSPFTRAAPPGSPFTRAAPPGSPFTRATPPGSPFTRATPPGSPFTRATPPGSPFTRASLKSSKNDSASYFRRKEKRLRFTIRRLVKAQSFYWTVLCLVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLTEMSMKMYGLGPRNYFHSSFNCFDFGVIIGSIFEVIWAVVKPGASYGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGMFNFEEETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESHGGVRWGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEVAITKKMALQMVKEVKEVSPMSAANISITAKEQQKSLKMMSVWEQRTTQLRRHNLRASSEALYSELDPEERLRMSSALHIRPDMKTHLDRPLVVKPHDGPGPMGHLKPQTPEDTDNPGDPAGPQPRRHHRDRERPIDEANENGDPGKEGRHHVHHSRSKYPNGTRGKEGKSERSHSREGGRRHHHQTSVDEGGGGGTGTGERVHRHHHSHRQAREGNSTVNSGRKDERRSRHKDGRSGERCSRGENGANGERRRHKTHSYRGQSTLAGEEHSERERGKGHRDRYVESEGDSMATSTQQDPGDQRWGTEITEDSDNQRNVRRTGWTAVHIPPVTITSLPGETTLIPMNSIDCETLPMNEEKKTLEVLTRNGPRPILPYSSMFVFGQTNMVRRLCHYIVSLRYFEMCILVVISMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGPPQGVPRGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVIAVQLFKGKFFYCSDESKGLEKDCRGHFLDYDKDEVAAQPRVWKKYEFHYDNVLWAFLTLFTVSTGEGWPDVLKHSVDATFEDQGPSPGYRIEISIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPENVQSFQYRMWKFVVSPPFEYAIMIMIALNTVVLMMKFHGAPDFYEAMLKHFNIVFTALFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDIVVTEINTTDRLLNLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIELNEDTSITHHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSHRACDERSGNLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIKYLDMYQILLHMSPPLGLGKKCPPRVAYKRLVKMNMPIADDNTVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSQVWPSLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLKEQQQQQQQQNATGSQGKVGALFKPILPLTHKQEKVKEKAMTNVEPPCELLPQPQSQALPRPSSTLLNNGGTLPGHDGTMKGSSSWVSEKSREVHRPRIKTPVQRGQSEDVPNLTIPQESVEMRKMDTSCSTVPGPGSGLESQGRAVSMPRLNAELQRSKSRHSPGTLLAPVPDCSPMKRSASTHAPQRPQEVNLHEHTLERPSTEERRHHHRHHHRCHHHRERDKKQRSLDRTPSTIGAPGDPAAEPSSRDRVQDRGRSHERKNHSTAGEKQRYYSCDRYSSREHCHTTKSATTSCATSPSEVQEPSINKQGSGSVKGSPVMLTSGAITPSRGRRQLPQTPLTPRPGVTFKTANSSPVHFVSAQCGSGLPTLSPGRLSRGLSEHNALLHSGTSHIPSPITRISSEPFLGYSHSDPQGQGGPYCNLRDELLSPYGARAHSPRTARPQMRPGALHLPPPPQQRRAVPNGYHFTFGGNGNASLGSGSGARPPRYYHEAEEDEWC

Target ORF information:

RefSeq Version	XM_014171613.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014171613.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCTAA GGAGCAGCAG 
AAGTCTCTGA AGATGATGTC AGTGTGGGAG CAGCGCACCA CTCAGCTGAG GAGACACAAC 
CTGCGGGCCA GCAGTGAGGC CCTGTACAGC GAGCTGGACC CTGAGGAGAG GCTACGCATG 
TCCTCCGCCC TGCACATCCG CCCTGACATG AAGACCCACC TGGACCGGCC CCTGGTCGTG 
AAGCCCCACG ACGGCCCCGG GCCCATGGGC CACCTCAAGC CCCAGACCCC TGAGGACACG 
GATAACCCTG GGGACCCGGC CGGCCCCCAG CCACGTAGGC ACCACCGGGA CAGGGAGAGA 
CCCATCGACG AGGCCAATGA GAATGGAGAT CCAGGCAAAG AGGGTAGGCA CCATGTCCAT 
CACAGTCGCA GCAAGTACCC CAATGGGACT CGGGGTAAGG AGGGGAAGAG TGAGCGGTCG 
CATAGCCGAG AGGGTGGCAG GAGGCACCAC CACCAGACCT CAGTGGACGA GGGAGGTGGA 
GGGGGAACTG GGACGGGAGA GAGAGTGCAC CGCCACCACC ACTCCCACAG ACAGGCCAGA 
GAGGGTAACA GCACTGTCAA CAGCGGACGC AAGGATGAGC GCAGGTCACG CCACAAAGAT 
GGACGCTCGG GAGAGCGATG CTCCAGGGGA GAGAACGGGG CCAATGGGGA GAGGAGGAGA 
CACAAGACCC ACAGTTACAG GGGCCAGTCC ACACTGGCGG GAGAGGAGCA CAGTGAGAGG 
GAGAGGGGGA AGGGCCACAG GGACAGATAT GTTGAGTCTG AGGGCGACTC CATGGCCACT 
AGCACCCAGC AGGATCCTGG AGATCAGAGG TGGGGCACAG AGATAACTGA GGACTCAGAC 
AACCAGAGGA ACGTCAGACG GACAGGATGG ACGGCCGTCC ACATACCCCC TGTCACCATC 
ACCTCCCTGC CTGGAGAGAC CACCCTTATC CCCATGAACA GTATAGACTG TGAGACATTG 
CCTATGAACG AGGAGAAGAA GACCCTGGAG GTGTTGACTC GGAACGGACC CAGACCCATC 
CTCCCCTACA GCTCCATGTT TGTGTTTGGC CAGACCAACA TGGTGCGGCG TCTGTGTCAT 
TACATTGTGA GCCTGCGGTA CTTTGAGATG TGTATCCTGG TAGTGATATC TATGAGTAGC 
ATTGCATTGG CTGCTGAGGA CCCTGTCCAG GCCAATGCTC CACGCAACAA TGTGCTCAAG 
TATCTGGACT ACGTCTTTAC GGGAGTTTTC ACATTTGAGA TGGTGATTAA GATGGTTGAC 
CTGGGCCTTC TGCTCCATCC TGGTTCTTAC TTCAGAGATC TCTGGAACAT TCTGGACTTC 
ATTGTGGTCA GTGGAGCACT GGTGGCATTT GCATTTTCGA GCTTCATGGG ACCGCCACAA 
GGTGTGCCCA GAGGGTCCAA AGGAAAGGAC ATCAGCACCA TCAAGTCCCT CAGAGTTCTG 
CGAGTCTTAC GGCCCCTCAA GACCATCAAG CGCCTGCCAA AGCTGAAGGC TGTGTTTGAC 
TGTGTGGTCA ACTCCCTGAA GAACGTCTTC AACATCCTCA TTGTCTACAT GCTCTTCATG 
TTCATCTTCG CTGTCATCGC TGTGCAGCTG TTCAAGGGAA AGTTCTTTTA CTGCTCCGAT 
GAGTCCAAGG GACTGGAAAA GGACTGCAGA GGTCATTTCC TGGACTACGA TAAGGATGAG 
GTGGCCGCCC AGCCTCGGGT GTGGAAGAAA TATGAGTTCC ACTATGACAA CGTGCTGTGG 
GCCTTCCTCA CTCTCTTCAC TGTGTCCACT GGAGAGGGCT GGCCCGATGT TCTGAAGCAC 
TCTGTGGATG CTACCTTTGA GGACCAGGGT CCCAGTCCAG GCTACCGTAT AGAGATCTCC 
ATCTTCTACG TGGTCTACTT CGTGGTCTTT CCCTTCTTCT TCGTCAACAT CTTTGTGGCC 
TTGATCATCA TTACCTTCCA GGAGCAAGGG GACAAGGCCA TGTCAGAGTG CAGCCTGGAG 
AAAAACGAGA GGGCCTGCAT TGACTTTGCC ATCAACGCCA AGCCACTGAC GCGTTACATG 
CCAGAGAACG TGCAGTCCTT CCAGTACCGG ATGTGGAAGT TTGTGGTGTC ACCCCCGTTT 
GAGTACGCCA TTATGATCAT GATCGCCCTG AACACCGTGG TGCTCATGAT GAAGTTCCAT 
GGAGCTCCAG ACTTCTACGA GGCCATGTTG AAGCACTTCA ACATCGTCTT CACTGCCCTC 
TTCTCTCTGG AGTGCATTCT GAAGATCATC GCCTTCGGAC CACTGAACTA CCTGAAGGAT 
GCCTGGAACG TGTTTGACTT TGTGACCGTG CTGGGAAGTA TAACTGACAT CGTGGTCACA 
GAAATCAATA CTACGGACCG GCTTTTGAAC CTGAGTTTTC TGAGGCTGTT CAGAGCAGCT 
CGTCTCATCA AACTACTGAG GCAAGGCTAC ACCATCCGTA TCCTGCTGTG GACCTTCGTC 
CAGTCCTTTA AGGCCCTGCC CTATGTTTGC CTTCTGATAG CAATGCTGTT CTTCATCTAT 
GCCATCATCG GTATGCAGGT GTTTGGAAAC ATTGAGCTCA ACGAGGACAC ATCCATCACT 
CACCACAACA ACTTCCGCAC TTTTCTTCAG GCTCTCATGC TACTGTTCAG GAGTGCCACA 
GGGGAGGCGT GGCATGAGAT CATGCTGTCG TGTTTGAGTC ACAGAGCCTG TGATGAGAGG 
TCAGGCAACC TGGGGAAGGA GTGTGGCAGC GACTTTGCCT ATTTCTACTT TGTGTCCTTC 
ATCTTCCTCT GCTCTTTCCT GATGCTTAAT CTGTTTGTGG CTGTGATCAT GGATAACTTT 
GAATACCTGA CTCGAGACTC TTCTATACTG GGGCCTCATC ATCTGGATGA GTTCATCCGT 
GTTTGGGCTG AGTACGACCC TGCTGCCTGT GGCCGTATCA AGTACCTCGA TATGTATCAG 
ATACTCCTCC ACATGTCCCC ACCCTTAGGT TTGGGAAAGA AATGTCCGCC AAGAGTCGCC 
TACAAGCGGC TAGTCAAGAT GAACATGCCC ATCGCTGATG ACAACACAGT CCACTTCACC 
TCCACACTCA TGGCCCTGAT CCGGACGGCC CTGGAGATCA AACTGGCCTC AGGGATGGTT 
GCCCAGCGTC TCTCTGATGC TGAACTGAAG AAAGAGCTCT CCCAGGTTTG GCCAAGCCTC 
TCACAGAAGA CTATGGATCT GTTGGTGACA CCACACAAAC CCAATGAGCT TACTGTTGGG 
AAGGTGTATG CAGCCCTGAT GATCTTTGAC TACTACAAGC AGAACCGAGC CAAGAGGCTC 
AAGGAACAGC AACAGCAGCA GCAGCAACAG AATGCTACTG GCTCCCAGGG AAAGGTGGGG 
GCTCTGTTTA AACCAATACT GCCCCTGACT CACAAGCAGG AGAAAGTGAA GGAGAAGGCC 
ATGACCAACG TGGAGCCTCC CTGTGAGCTA CTGCCTCAGC CCCAGTCTCA GGCCCTACCT 
AGACCCAGCT CCACCCTGCT CAACAACGGA GGCACACTGC CAGGCCATGA CGGCACCATG 
AAAGGATCAT CCTCCTGGGT GTCAGAGAAG TCTAGAGAGG TCCACAGACC CAGGATTAAG 
ACACCTGTCC AAAGGGGCCA GTCTGAGGAT GTCCCAAACC TAACCATCCC CCAGGAGTCA 
GTGGAGATGA GGAAGATGGA CACCAGTTGC AGCACAGTGC CTGGTCCTGG GTCTGGACTG 
GAGAGCCAGG GAAGAGCTGT GTCCATGCCC CGCCTCAATG CAGAGCTGCA GAGGAGCAAG 
TCGCGCCACT CCCCGGGGAC CCTGCTGGCA CCTGTTCCTG ACTGCAGCCC AATGAAACGC 
TCTGCGTCCA CCCACGCCCC CCAGCGCCCC CAGGAGGTGA ACCTGCATGA ACACACCCTG 
GAGAGGCCCA GCACCGAAGA AAGGCGTCAC CATCACCGCC ACCACCACCG CTGCCACCAC 
CACCGAGAGA GGGACAAGAA GCAGCGGTCA CTGGACAGGA CGCCAAGCAC CATTGGTGCA 
CCAGGTGACC CGGCTGCAGA GCCCTCGTCT AGAGACAGGG TGCAGGACCG CGGCCGCTCC 
CATGAGAGGA AGAACCACTC GACGGCGGGG GAGAAGCAGC GCTACTACTC TTGCGATCGC 
TACAGCAGCA GGGAACACTG CCACACCACC AAATCTGCCA CCACTAGCTG TGCCACCTCC 
CCCAGCGAGG TGCAGGAACC CAGCATCAAC AAGCAGGGTA GTGGTTCGGT TAAAGGCAGC 
CCAGTGATGT TGACCTCAGG GGCTATCACA CCTAGCCGTG GACGCAGGCA GCTCCCACAG 
ACCCCCCTGA CCCCCCGCCC CGGGGTGACG TTCAAGACTG CAAACTCCTC CCCTGTGCAC 
TTTGTGTCTG CCCAGTGTGG CAGCGGCCTG CCCACCCTGA GTCCTGGCCG GCTGAGCCGT 
GGGCTGTCTG AGCACAACGC CCTGCTGCAT AGTGGCACCT CTCACATCCC CTCCCCCATC 
ACCCGCATCA GCTCTGAGCC CTTCCTGGGC TACAGTCACA GTGACCCCCA AGGCCAGGGT 
GGGCCGTACT GCAACCTCCG GGACGAGCTG CTGAGCCCTT ATGGTGCCAG AGCACACTCC 
CCTCGGACTG CACGTCCTCA AATGAGGCCG GGGGCCCTCC ACCTGCCTCC GCCACCCCAG 
CAGAGACGTG CGGTGCCCAA CGGATACCAC TTCACCTTTG GTGGTAATGG CAACGCCAGC 
CTGGGCTCTG GCTCTGGTGC CAGGCCACCC AGGTACTATC ACGAGGCAGA GGAGGATGAA 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106585428 ( XM_014171614.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014027089.1 Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X8, mRNA.

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CloneID	OAt67171
Clone ID Related Accession (Same CDS sequence)	XM_014171614.1
Accession Version	XM_014171614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	voltage-dependent N-type calcium channel subunit alpha-1B-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012340658.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCTAA GGAGCAGCAG 
AAGTCTCTGA AGATGATGTC AGTGTGGGAG CAGCGCACCA CTCAGCTGAG GAGACACAAC 
CTGCGGGCCA GCAGTGAGGC CCTGTACAGC GAGCTGGACC CTGAGGAGAG GCTACGCATG 
TCCTCCGCCC TGCACATCCG CCCTGACATG AAGACCCACC TGGACCGGCC CCTGGTCGTG 
AAGCCCCACG ACGGCCCCGG GCCCATGGGC CACCTCAAGC CCCAGACCCC TGAGGACACG 
GATAACCCTG GGGACCCGGC CGGCCCCCAG CCACGTAGGC ACCACCGGGA CAGGGAGAGA 
CCCATCGACG AGGCCAATGA GAATGGAGAT CCAGGCAAAG AGGGTAGGCA CCATGTCCAT 
CACAGTCGCA GCAAGTACCC CAATGGGACT CGGGGTAAGG AGGGGAAGAG TGAGCGGTCG 
CATAGCCGAG AGGGTGGCAG GAGGCACCAC CACCAGACCT CAGTGGACGA GGGAGGTGGA 
GGGGGAACTG GGACGGGAGA GAGAGTGCAC CGCCACCACC ACTCCCACAG ACAGGCCAGA 
GAGGGTAACA GCACTGTCAA CAGCGGACGC AAGGATGAGC GCAGGTCACG CCACAAAGAT 
GGACGCTCGG GAGAGCGATG CTCCAGGGGA GAGAACGGGG CCAATGGGGA GAGGAGGAGA 
CACAAGACCC ACAGTTACAG GGGCCAGTCC ACACTGGCGG GAGAGGAGCA CAGTGAGAGG 
GAGAGGGGGA AGGGCCACAG GGACAGATAT GTTGAGTCTG AGGGCGACTC CATGGCCACT 
AGCACCCAGC AGGATCCTGG AGATCAGAGG TGGGGCACAG AGATAACTGA GGACTCAGAC 
AACCAGAGGA ACGTCAGACG GACAGGATGG ACGGCCGTCC ACATACCCCC TGTCACCATC 
ACCTCCCTGC CTGGAGAGAC CACCCTTATC CCCATGAACA GTATAGACTG TGAGACATTG 
CCTATGAACG AGGAGAAGAA GACCCTGGAG GTGTTGACTC GGAACGGACC CAGACCCATC 
CTCCCCTACA GCTCCATGTT TGTGTTTGGC CAGACCAACA TGGTGCGGCG TCTGTGTCAT 
TACATTGTGA GCCTGCGGTA CTTTGAGATG TGTATCCTGG TAGTGATATC TATGAGTAGC 
ATTGCATTGG CTGCTGAGGA CCCTGTCCAG GCCAATGCTC CACGCAACAA TGTGCTCAAG 
TATCTGGACT ACGTCTTTAC GGGAGTTTTC ACATTTGAGA TGGTGATTAA GATGGTTGAC 
CTGGGCCTTC TGCTCCATCC TGGTTCTTAC TTCAGAGATC TCTGGAACAT TCTGGACTTC 
ATTGTGGTCA GTGGAGCACT GGTGGCATTT GCATTTTCGA GCTTCATGGG ACCGCCACAA 
GGTGTGCCCA GAGGGTCCAA AGGAAAGGAC ATCAGCACCA TCAAGTCCCT CAGAGTTCTG 
CGAGTCTTAC GGCCCCTCAA GACCATCAAG CGCCTGCCAA AGCTGAAGGC TGTGTTTGAC 
TGTGTGGTCA ACTCCCTGAA GAACGTCTTC AACATCCTCA TTGTCTACAT GCTCTTCATG 
TTCATCTTCG CTGTCATCGC TGTGCAGCTG TTCAAGGGAA AGTTCTTTTA CTGCTCCGAT 
GAGTCCAAGG GACTGGAAAA GGACTGCAGA GGTCATTTCC TGGACTACGA TAAGGATGAG 
GTGGCCGCCC AGCCTCGGGT GTGGAAGAAA TATGAGTTCC ACTATGACAA CGTGCTGTGG 
GCCTTCCTCA CTCTCTTCAC TGTGTCCACT GGAGAGGGCT GGCCCGATGT TCTGAAGCAC 
TCTGTGGATG CTACCTTTGA GGACCAGGGT CCCAGTCCAG GCTACCGTAT AGAGATCTCC 
ATCTTCTACG TGGTCTACTT CGTGGTCTTT CCCTTCTTCT TCGTCAACAT CTTTGTGGCC 
TTGATCATCA TTACCTTCCA GGAGCAAGGG GACAAGGCCA TGTCAGAGTG CAGCCTGGAG 
AAAAACGAGA GGGCCTGCAT TGACTTTGCC ATCAACGCCA AGCCACTGAC GCGTTACATG 
CCAGAGAACG TGCAGTCCTT CCAGTACCGG ATGTGGAAGT TTGTGGTGTC ACCCCCGTTT 
GAGTACGCCA TTATGATCAT GATCGCCCTG AACACCGTGG TGCTCATGAT GAAGTTCCAT 
GGAGCTCCAG ACTTCTACGA GGCCATGTTG AAGCACTTCA ACATCGTCTT CACTGCCCTC 
TTCTCTCTGG AGTGCATTCT GAAGATCATC GCCTTCGGAC CACTGAACTA CCTGAAGGAT 
GCCTGGAACG TGTTTGACTT TGTGACCGTG CTGGGAAGTA TAACTGACAT CGTGGTCACA 
GAAATCAATA CTACGGACCG GCTTTTGAAC CTGAGTTTTC TGAGGCTGTT CAGAGCAGCT 
CGTCTCATCA AACTACTGAG GCAAGGCTAC ACCATCCGTA TCCTGCTGTG GACCTTCGTC 
CAGTCCTTTA AGGCCCTGCC CTATGTTTGC CTTCTGATAG CAATGCTGTT CTTCATCTAT 
GCCATCATCG GTATGCAGGT GTTTGGAAAC ATTGAGCTCA ACGAGGACAC ATCCATCACT 
CACCACAACA ACTTCCGCAC TTTTCTTCAG GCTCTCATGC TACTGTTCAG GAGTGCCACA 
GGGGAGGCGT GGCATGAGAT CATGCTGTCG TGTTTGAGTC ACAGAGCCTG TGATGAGAGG 
TCAGGCAACC TGGGGAAGGA GTGTGGCAGC GACTTTGCCT ATTTCTACTT TGTGTCCTTC 
ATCTTCCTCT GCTCTTTCCT GATGCTTAAT CTGTTTGTGG CTGTGATCAT GGATAACTTT 
GAATACCTGA CTCGAGACTC TTCTATACTG GGGCCTCATC ATCTGGATGA GTTCATCCGT 
GTTTGGGCTG AGTACGACCC TGCTGCCTGC GGACGGATTC GCTATAACGA TATGTACAAC 
TTGTTACGAA TTATCTCACC CCCCCTTGGC TTAGGGAAGA ACTGCCCTAA TAGGGTAGCA 
TACAAGCGGC TAGTCAAGAT GAACATGCCC ATCGCTGATG ACAACACAGT CCACTTCACC 
TCCACACTCA TGGCCCTGAT CCGGACGGCC CTGGAGATCA AACTGGCCTC AGGGATGGTT 
GCCCAGCGTC TCTCTGATGC TGAACTGAAG AAAGAGCTCT CCCAGGTTTG GCCAAGCCTC 
TCACAGAAGA CTATGGATCT GTTGGTGACA CCACACAAAC CCAATGAGCT TACTGTTGGG 
AAGGTGTATG CAGCCCTGAT GATCTTTGAC TACTACAAGC AGAACCGAGC CAAGAGGCTC 
AAGGAACAGC AACAGCAGCA GCAGCAACAG AATGCTACTG GCTCCCAGGG AAAGGTGGGG 
GCTCTGTTTA AACCAATACT GCCCCTGACT CACAAGCAGG AGAAAGTGAA GGAGAAGGCC 
ATGACCAACG TGGAGCCTCC CTGTGAGCTA CTGCCTCAGC CCCAGTCTCA GGCCCTACCT 
AGACCCAGCT CCACCCTGCT CAACAACGGA GGCACACTGC CAGGCCATGA CGGCACCATG 
AAAGGATCAT CCTCCTGGGT GTCAGAGAAG TCTAGAGAGG TCCACAGACC CAGGATTAAG 
ACACCTGTCC AAAGGGGCCA GTCTGAGGAT GTCCCAAACC TAACCATCCC CCAGGAGTCA 
GTGGAGATGA GGAAGATGGA CACCAGTTGC AGCACAGTGC CTGGTCCTGG GTCTGGACTG 
GAGAGCCAGG GAAGAGCTGT GTCCATGCCC CGCCTCAATG CAGAGCTGCA GAGGAGCAAG 
TCGCGCCACT CCCCGGGGAC CCTGCTGGCA CCTGTTCCTG ACTGCAGCCC AATGAAACGC 
TCTGCGTCCA CCCACGCCCC CCAGCGCCCC CAGGAGGTGA ACCTGCATGA ACACACCCTG 
GAGAGGCCCA GCACCGAAGA AAGGCGTCAC CATCACCGCC ACCACCACCG CTGCCACCAC 
CACCGAGAGA GGGACAAGAA GCAGCGGTCA CTGGACAGGA CGCCAAGCAC CATTGGTGCA 
CCAGGTGACC CGGCTGCAGA GCCCTCGTCT AGAGACAGGG TGCAGGACCG CGGCCGCTCC 
CATGAGAGGA AGAACCACTC GACGGCGGGG GAGAAGCAGC GCTACTACTC TTGCGATCGC 
TACAGCAGCA GGGAACACTG CCACACCACC AAATCTGCCA CCACTAGCTG TGCCACCTCC 
CCCAGCGAGG TGCAGGAACC CAGCATCAAC AAGCAGGGTA GTGGTTCGGT TAAAGGCAGC 
CCAGTGATGT TGACCTCAGG GGCTATCACA CCTAGCCGTG GACGCAGGCA GCTCCCACAG 
ACCCCCCTGA CCCCCCGCCC CGGGGTGACG TTCAAGACTG CAAACTCCTC CCCTGTGCAC 
TTTGTGTCTG CCCAGTGTGG CAGCGGCCTG CCCACCCTGA GTCCTGGCCG GCTGAGCCGT 
GGGCTGTCTG AGCACAACGC CCTGCTGCAT AGTGGCACCT CTCACATCCC CTCCCCCATC 
ACCCGCATCA GCTCTGAGCC CTTCCTGGGC TACAGTCACA GTGACCCCCA AGGCCAGGGT 
GGGCCGTACT GCAACCTCCG GGACGAGCTG CTGAGCCCTT ATGGTGCCAG AGCACACTCC 
CCTCGGACTG CACGTCCTCA AATGAGGCCG GGGGCCCTCC ACCTGCCTCC GCCACCCCAG 
CAGAGACGTG CGGTGCCCAA CGGATACCAC TTCACCTTTG GTGGTAATGG CAACGCCAGC 
CTGGGCTCTG GCTCTGGTGC CAGGCCACCC AGGTACTATC ACGAGGCAGA GGAGGATGAA 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014027089.1
CDS	635..7903
Translation	MARFEEELSNRRGGVCQAGPPRVAGRPAGHPSGPRVYKQSMAQRARTMAIYNPIPVKQSCLTVNRSLFIFSEDCIIRKYAKKITEWPPFEYLILATIIANCIVLALEQHLPASDKTPMSERLDDTEPYFIAIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGMLATVGADFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGMLLFFAILMFAIIGLDFYMGKFHRTCFRTDTGEQAAEFPCGMEAPARTCENGTVCQEYWIGPNYGITNFDNILFAILTVFQCITMEGWVDILYNANDASGNTWNWIYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVMLAEEDKHAEEKSPLDGAWYKRKHNNPVLKRAKKSKNDLINAEEGEDHFTDVSSVGSPFTRATPPGSPFTRAAPPGSPFTRAAPPGSPFTRATPPGSPFTRATPPGSPFTRATPPGSPFTRASLKSSKNDSASYFRRKEKRLRFTIRRLVKAQSFYWTVLCLVGLNTLCVAIVHYDQPEWLTYALYLAEFVFLGLFLTEMSMKMYGLGPRNYFHSSFNCFDFGVIIGSIFEVIWAVVKPGASYGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGMFNFEEETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESHGGVRWGMFSSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEVAITKKMALQMVKEVKEVSPMSAANISITAKEQQKSLKMMSVWEQRTTQLRRHNLRASSEALYSELDPEERLRMSSALHIRPDMKTHLDRPLVVKPHDGPGPMGHLKPQTPEDTDNPGDPAGPQPRRHHRDRERPIDEANENGDPGKEGRHHVHHSRSKYPNGTRGKEGKSERSHSREGGRRHHHQTSVDEGGGGGTGTGERVHRHHHSHRQAREGNSTVNSGRKDERRSRHKDGRSGERCSRGENGANGERRRHKTHSYRGQSTLAGEEHSERERGKGHRDRYVESEGDSMATSTQQDPGDQRWGTEITEDSDNQRNVRRTGWTAVHIPPVTITSLPGETTLIPMNSIDCETLPMNEEKKTLEVLTRNGPRPILPYSSMFVFGQTNMVRRLCHYIVSLRYFEMCILVVISMSSIALAAEDPVQANAPRNNVLKYLDYVFTGVFTFEMVIKMVDLGLLLHPGSYFRDLWNILDFIVVSGALVAFAFSSFMGPPQGVPRGSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVIAVQLFKGKFFYCSDESKGLEKDCRGHFLDYDKDEVAAQPRVWKKYEFHYDNVLWAFLTLFTVSTGEGWPDVLKHSVDATFEDQGPSPGYRIEISIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPENVQSFQYRMWKFVVSPPFEYAIMIMIALNTVVLMMKFHGAPDFYEAMLKHFNIVFTALFSLECILKIIAFGPLNYLKDAWNVFDFVTVLGSITDIVVTEINTTDRLLNLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIELNEDTSITHHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSHRACDERSGNLGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRIRYNDMYNLLRIISPPLGLGKNCPNRVAYKRLVKMNMPIADDNTVHFTSTLMALIRTALEIKLASGMVAQRLSDAELKKELSQVWPSLSQKTMDLLVTPHKPNELTVGKVYAALMIFDYYKQNRAKRLKEQQQQQQQQNATGSQGKVGALFKPILPLTHKQEKVKEKAMTNVEPPCELLPQPQSQALPRPSSTLLNNGGTLPGHDGTMKGSSSWVSEKSREVHRPRIKTPVQRGQSEDVPNLTIPQESVEMRKMDTSCSTVPGPGSGLESQGRAVSMPRLNAELQRSKSRHSPGTLLAPVPDCSPMKRSASTHAPQRPQEVNLHEHTLERPSTEERRHHHRHHHRCHHHRERDKKQRSLDRTPSTIGAPGDPAAEPSSRDRVQDRGRSHERKNHSTAGEKQRYYSCDRYSSREHCHTTKSATTSCATSPSEVQEPSINKQGSGSVKGSPVMLTSGAITPSRGRRQLPQTPLTPRPGVTFKTANSSPVHFVSAQCGSGLPTLSPGRLSRGLSEHNALLHSGTSHIPSPITRISSEPFLGYSHSDPQGQGGPYCNLRDELLSPYGARAHSPRTARPQMRPGALHLPPPPQQRRAVPNGYHFTFGGNGNASLGSGSGARPPRYYHEAEEDEWC

Target ORF information:

RefSeq Version	XM_014171614.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar voltage-dependent N-type calcium channel subunit alpha-1B-like (LOC106585428), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014171614.1

ORF Insert Sequence:

ATGGCTCGCT TCGAAGAGGA ACTATCTAAC CGGCGTGGAG GGGTCTGTCA AGCGGGCCCA 
CCAAGAGTGG CAGGTAGGCC AGCAGGCCAC CCAAGCGGCC CTAGGGTGTA CAAACAATCA 
ATGGCCCAAC GAGCCAGGAC AATGGCCATT TACAACCCCA TCCCTGTCAA GCAGAGCTGT 
CTTACTGTCA ACCGGTCTCT ATTCATCTTT AGTGAGGACT GTATCATACG GAAATATGCC 
AAAAAGATTA CCGAATGGCC TCCATTTGAA TACCTGATCC TGGCCACCAT TATAGCCAAC 
TGCATCGTCC TTGCCTTGGA GCAGCACCTA CCAGCCAGCG ACAAGACCCC CATGTCAGAG 
CGCTTGGATG ACACAGAGCC CTATTTTATT GCAATATTTT GTTTTGAAGC TGGCATTAAG 
ATCATCGCCC TTGGTTTTGC GTTTCACAAA GGCTCCTATC TTCGGAATGG CTGGAATGTA 
ATGGACTTTG TGGTGGTGCT AACAGGGATG CTGGCTACAG TGGGAGCAGA CTTTGACTTG 
AGAACTCTGA GGGCAGTACG GGTCCTGAGG CCACTGAAAC TGGTGTCTGG AATCCCTAGT 
CTTCAGGTAG TTCTAAAGTC CATCATGAAA GCCATGGTGC CTCTGCTGCA GATTGGCATG 
CTGCTCTTCT TCGCTATACT CATGTTCGCA ATCATCGGCC TGGACTTTTA CATGGGCAAG 
TTCCACCGGA CCTGCTTCAG GACTGATACA GGTGAGCAGG CAGCAGAGTT CCCCTGTGGT 
ATGGAGGCTC CAGCGAGGAC CTGTGAAAAT GGAACTGTGT GTCAGGAATA CTGGATCGGC 
CCCAACTACG GCATTACCAA CTTTGACAAC ATCCTCTTTG CTATTCTGAC CGTCTTCCAG 
TGCATCACCA TGGAGGGATG GGTGGACATC CTCTATAACG CCAACGATGC CTCAGGGAAC 
ACCTGGAACT GGATCTACTT CATCCCCCTC ATCATCATCG GATCCTTCTT CATGCTCAAC 
CTGGTCCTGG GAGTCCTGTC TGGGGAGTTT GCCAAGGAGC GTGAGCGTGT GGAGAAGAGG 
CAGGAGTTTC TGAAGCTTCG CAGGCAGCAG CAGATCGAGA GGGAGCTGAC TGGATACCTG 
GAATGGATAT GCAAGGCAGA GGAGGTGATG TTGGCAGAGG AGGACAAGCA TGCTGAAGAG 
AAGTCTCCTC TGGATGGAGC GTGGTACAAG AGGAAACACA ACAACCCAGT GTTAAAAAGA 
GCCAAGAAGA GCAAGAATGA CCTGATCAAC GCTGAGGAGG GCGAAGACCA CTTCACTGAC 
GTCTCATCTG TTGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC GTTCACCCGG 
GCTGCTCCCC CAGGGTCTCC GTTCACCCGG GCTGCTCCCC CAGGGTCTCC GTTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCTACTCCCC CAGGGTCTCC ATTCACCCGG 
GCTACTCCCC CAGGGTCTCC GTTCACCCGG GCCAGCCTGA AGAGCAGCAA GAACGACAGT 
GCTTCCTACT TCCGGAGGAA AGAGAAGCGG CTCCGCTTCA CCATCAGACG CCTGGTCAAG 
GCCCAGAGCT TCTATTGGAC AGTCCTGTGT CTGGTGGGCC TCAACACACT GTGTGTGGCC 
ATCGTCCATT ATGACCAGCC TGAGTGGCTC ACCTATGCGC TGTATTTGGC AGAGTTTGTA 
TTCCTGGGCT TGTTTCTGAC TGAGATGTCC ATGAAAATGT ATGGACTAGG ACCTAGGAAC 
TACTTCCATT CCTCTTTCAA TTGTTTTGAT TTTGGAGTGA TTATAGGCAG TATTTTTGAG 
GTGATCTGGG CAGTTGTGAA ACCTGGGGCC TCCTACGGCA TCAGCGTCCT GCGAGCTCTC 
CGTCTGCTCA GGATTTTCAA AGTCACCAAG TACTGGAACT CTTTGAGGAA CCTGGTGGTC 
TCTTTACTCA ACTCCATGAA GTCCATTATC AGCCTGCTCT TCCTCCTCTT CCTCTTCATC 
GTGGTGTTCG CCCTACTGGG CATGCAGCTC TTTGGGGGAA TGTTTAATTT TGAGGAGGAG 
ACACCAACAA CAAACTTCGA CACCTTTCCA GCAGCCATTC TCACAGTTTT CCAGATACTG 
ACAGGAGAGG ACTGGAATGC AGTGATGTAT CATGGGATAG AGTCCCATGG GGGCGTTCGG 
TGGGGAATGT TCTCCTCTAT CTACTTCATC GTTCTCACCC TGTTTGGAAA CTACACTCTG 
CTCAATGTCT TCTTGGCCAT TGCTGTTGAC AACCTTGCAA ACGCACAGGA GCTCACTAAG 
GACGAGGAGG AGCAGGAGGT GGCCATCACT AAGAAGATGG CCCTCCAGAT GGTCAAGGAG 
GTGAAGGAGG TCAGCCCCAT GTCGGCAGCT AACATCTCCA TCACAGCTAA GGAGCAGCAG 
AAGTCTCTGA AGATGATGTC AGTGTGGGAG CAGCGCACCA CTCAGCTGAG GAGACACAAC 
CTGCGGGCCA GCAGTGAGGC CCTGTACAGC GAGCTGGACC CTGAGGAGAG GCTACGCATG 
TCCTCCGCCC TGCACATCCG CCCTGACATG AAGACCCACC TGGACCGGCC CCTGGTCGTG 
AAGCCCCACG ACGGCCCCGG GCCCATGGGC CACCTCAAGC CCCAGACCCC TGAGGACACG 
GATAACCCTG GGGACCCGGC CGGCCCCCAG CCACGTAGGC ACCACCGGGA CAGGGAGAGA 
CCCATCGACG AGGCCAATGA GAATGGAGAT CCAGGCAAAG AGGGTAGGCA CCATGTCCAT 
CACAGTCGCA GCAAGTACCC CAATGGGACT CGGGGTAAGG AGGGGAAGAG TGAGCGGTCG 
CATAGCCGAG AGGGTGGCAG GAGGCACCAC CACCAGACCT CAGTGGACGA GGGAGGTGGA 
GGGGGAACTG GGACGGGAGA GAGAGTGCAC CGCCACCACC ACTCCCACAG ACAGGCCAGA 
GAGGGTAACA GCACTGTCAA CAGCGGACGC AAGGATGAGC GCAGGTCACG CCACAAAGAT 
GGACGCTCGG GAGAGCGATG CTCCAGGGGA GAGAACGGGG CCAATGGGGA GAGGAGGAGA 
CACAAGACCC ACAGTTACAG GGGCCAGTCC ACACTGGCGG GAGAGGAGCA CAGTGAGAGG 
GAGAGGGGGA AGGGCCACAG GGACAGATAT GTTGAGTCTG AGGGCGACTC CATGGCCACT 
AGCACCCAGC AGGATCCTGG AGATCAGAGG TGGGGCACAG AGATAACTGA GGACTCAGAC 
AACCAGAGGA ACGTCAGACG GACAGGATGG ACGGCCGTCC ACATACCCCC TGTCACCATC 
ACCTCCCTGC CTGGAGAGAC CACCCTTATC CCCATGAACA GTATAGACTG TGAGACATTG 
CCTATGAACG AGGAGAAGAA GACCCTGGAG GTGTTGACTC GGAACGGACC CAGACCCATC 
CTCCCCTACA GCTCCATGTT TGTGTTTGGC CAGACCAACA TGGTGCGGCG TCTGTGTCAT 
TACATTGTGA GCCTGCGGTA CTTTGAGATG TGTATCCTGG TAGTGATATC TATGAGTAGC 
ATTGCATTGG CTGCTGAGGA CCCTGTCCAG GCCAATGCTC CACGCAACAA TGTGCTCAAG 
TATCTGGACT ACGTCTTTAC GGGAGTTTTC ACATTTGAGA TGGTGATTAA GATGGTTGAC 
CTGGGCCTTC TGCTCCATCC TGGTTCTTAC TTCAGAGATC TCTGGAACAT TCTGGACTTC 
ATTGTGGTCA GTGGAGCACT GGTGGCATTT GCATTTTCGA GCTTCATGGG ACCGCCACAA 
GGTGTGCCCA GAGGGTCCAA AGGAAAGGAC ATCAGCACCA TCAAGTCCCT CAGAGTTCTG 
CGAGTCTTAC GGCCCCTCAA GACCATCAAG CGCCTGCCAA AGCTGAAGGC TGTGTTTGAC 
TGTGTGGTCA ACTCCCTGAA GAACGTCTTC AACATCCTCA TTGTCTACAT GCTCTTCATG 
TTCATCTTCG CTGTCATCGC TGTGCAGCTG TTCAAGGGAA AGTTCTTTTA CTGCTCCGAT 
GAGTCCAAGG GACTGGAAAA GGACTGCAGA GGTCATTTCC TGGACTACGA TAAGGATGAG 
GTGGCCGCCC AGCCTCGGGT GTGGAAGAAA TATGAGTTCC ACTATGACAA CGTGCTGTGG 
GCCTTCCTCA CTCTCTTCAC TGTGTCCACT GGAGAGGGCT GGCCCGATGT TCTGAAGCAC 
TCTGTGGATG CTACCTTTGA GGACCAGGGT CCCAGTCCAG GCTACCGTAT AGAGATCTCC 
ATCTTCTACG TGGTCTACTT CGTGGTCTTT CCCTTCTTCT TCGTCAACAT CTTTGTGGCC 
TTGATCATCA TTACCTTCCA GGAGCAAGGG GACAAGGCCA TGTCAGAGTG CAGCCTGGAG 
AAAAACGAGA GGGCCTGCAT TGACTTTGCC ATCAACGCCA AGCCACTGAC GCGTTACATG 
CCAGAGAACG TGCAGTCCTT CCAGTACCGG ATGTGGAAGT TTGTGGTGTC ACCCCCGTTT 
GAGTACGCCA TTATGATCAT GATCGCCCTG AACACCGTGG TGCTCATGAT GAAGTTCCAT 
GGAGCTCCAG ACTTCTACGA GGCCATGTTG AAGCACTTCA ACATCGTCTT CACTGCCCTC 
TTCTCTCTGG AGTGCATTCT GAAGATCATC GCCTTCGGAC CACTGAACTA CCTGAAGGAT 
GCCTGGAACG TGTTTGACTT TGTGACCGTG CTGGGAAGTA TAACTGACAT CGTGGTCACA 
GAAATCAATA CTACGGACCG GCTTTTGAAC CTGAGTTTTC TGAGGCTGTT CAGAGCAGCT 
CGTCTCATCA AACTACTGAG GCAAGGCTAC ACCATCCGTA TCCTGCTGTG GACCTTCGTC 
CAGTCCTTTA AGGCCCTGCC CTATGTTTGC CTTCTGATAG CAATGCTGTT CTTCATCTAT 
GCCATCATCG GTATGCAGGT GTTTGGAAAC ATTGAGCTCA ACGAGGACAC ATCCATCACT 
CACCACAACA ACTTCCGCAC TTTTCTTCAG GCTCTCATGC TACTGTTCAG GAGTGCCACA 
GGGGAGGCGT GGCATGAGAT CATGCTGTCG TGTTTGAGTC ACAGAGCCTG TGATGAGAGG 
TCAGGCAACC TGGGGAAGGA GTGTGGCAGC GACTTTGCCT ATTTCTACTT TGTGTCCTTC 
ATCTTCCTCT GCTCTTTCCT GATGCTTAAT CTGTTTGTGG CTGTGATCAT GGATAACTTT 
GAATACCTGA CTCGAGACTC TTCTATACTG GGGCCTCATC ATCTGGATGA GTTCATCCGT 
GTTTGGGCTG AGTACGACCC TGCTGCCTGC GGACGGATTC GCTATAACGA TATGTACAAC 
TTGTTACGAA TTATCTCACC CCCCCTTGGC TTAGGGAAGA ACTGCCCTAA TAGGGTAGCA 
TACAAGCGGC TAGTCAAGAT GAACATGCCC ATCGCTGATG ACAACACAGT CCACTTCACC 
TCCACACTCA TGGCCCTGAT CCGGACGGCC CTGGAGATCA AACTGGCCTC AGGGATGGTT 
GCCCAGCGTC TCTCTGATGC TGAACTGAAG AAAGAGCTCT CCCAGGTTTG GCCAAGCCTC 
TCACAGAAGA CTATGGATCT GTTGGTGACA CCACACAAAC CCAATGAGCT TACTGTTGGG 
AAGGTGTATG CAGCCCTGAT GATCTTTGAC TACTACAAGC AGAACCGAGC CAAGAGGCTC 
AAGGAACAGC AACAGCAGCA GCAGCAACAG AATGCTACTG GCTCCCAGGG AAAGGTGGGG 
GCTCTGTTTA AACCAATACT GCCCCTGACT CACAAGCAGG AGAAAGTGAA GGAGAAGGCC 
ATGACCAACG TGGAGCCTCC CTGTGAGCTA CTGCCTCAGC CCCAGTCTCA GGCCCTACCT 
AGACCCAGCT CCACCCTGCT CAACAACGGA GGCACACTGC CAGGCCATGA CGGCACCATG 
AAAGGATCAT CCTCCTGGGT GTCAGAGAAG TCTAGAGAGG TCCACAGACC CAGGATTAAG 
ACACCTGTCC AAAGGGGCCA GTCTGAGGAT GTCCCAAACC TAACCATCCC CCAGGAGTCA 
GTGGAGATGA GGAAGATGGA CACCAGTTGC AGCACAGTGC CTGGTCCTGG GTCTGGACTG 
GAGAGCCAGG GAAGAGCTGT GTCCATGCCC CGCCTCAATG CAGAGCTGCA GAGGAGCAAG 
TCGCGCCACT CCCCGGGGAC CCTGCTGGCA CCTGTTCCTG ACTGCAGCCC AATGAAACGC 
TCTGCGTCCA CCCACGCCCC CCAGCGCCCC CAGGAGGTGA ACCTGCATGA ACACACCCTG 
GAGAGGCCCA GCACCGAAGA AAGGCGTCAC CATCACCGCC ACCACCACCG CTGCCACCAC 
CACCGAGAGA GGGACAAGAA GCAGCGGTCA CTGGACAGGA CGCCAAGCAC CATTGGTGCA 
CCAGGTGACC CGGCTGCAGA GCCCTCGTCT AGAGACAGGG TGCAGGACCG CGGCCGCTCC 
CATGAGAGGA AGAACCACTC GACGGCGGGG GAGAAGCAGC GCTACTACTC TTGCGATCGC 
TACAGCAGCA GGGAACACTG CCACACCACC AAATCTGCCA CCACTAGCTG TGCCACCTCC 
CCCAGCGAGG TGCAGGAACC CAGCATCAAC AAGCAGGGTA GTGGTTCGGT TAAAGGCAGC 
CCAGTGATGT TGACCTCAGG GGCTATCACA CCTAGCCGTG GACGCAGGCA GCTCCCACAG 
ACCCCCCTGA CCCCCCGCCC CGGGGTGACG TTCAAGACTG CAAACTCCTC CCCTGTGCAC 
TTTGTGTCTG CCCAGTGTGG CAGCGGCCTG CCCACCCTGA GTCCTGGCCG GCTGAGCCGT 
GGGCTGTCTG AGCACAACGC CCTGCTGCAT AGTGGCACCT CTCACATCCC CTCCCCCATC 
ACCCGCATCA GCTCTGAGCC CTTCCTGGGC TACAGTCACA GTGACCCCCA AGGCCAGGGT 
GGGCCGTACT GCAACCTCCG GGACGAGCTG CTGAGCCCTT ATGGTGCCAG AGCACACTCC 
CCTCGGACTG CACGTCCTCA AATGAGGCCG GGGGCCCTCC ACCTGCCTCC GCCACCCCAG 
CAGAGACGTG CGGTGCCCAA CGGATACCAC TTCACCTTTG GTGGTAATGG CAACGCCAGC 
CTGGGCTCTG GCTCTGGTGC CAGGCCACCC AGGTACTATC ACGAGGCAGA GGAGGATGAA 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC106585428 cDNA ORF clone, Salmo salar(Atlantic salmon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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