LOC106571391 gene cDNA ORF clone, Salmo salar(Atlantic salmon)

mRNA	Protein	Name
XM_014144424.1	XP_013999899.1	afadin-like isoform X7
XM_014144419.1	XP_013999894.1	afadin-like isoform X3
XM_014144425.1	XP_013999900.1	afadin-like isoform X8
XM_014144427.1	XP_013999902.1	afadin-like isoform X10
XM_014144430.1	XP_013999905.1	afadin-like isoform X13
XM_014144431.1	XP_013999906.1	afadin-like isoform X14
XM_014144420.1	XP_013999895.1	afadin-like isoform X4
XM_014144426.1	XP_013999901.1	afadin-like isoform X9
XM_014144418.1	XP_013999893.1	afadin-like isoform X2
XM_014144421.1	XP_013999896.1	afadin-like isoform X5
XM_014144423.1	XP_013999898.1	afadin-like isoform X6
XM_014144428.1	XP_013999903.1	afadin-like isoform X11
XM_014144417.1	XP_013999892.1	afadin-like isoform X1
XM_014144429.1	XP_013999904.1	afadin-like isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAt47150	XM_014144417.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47151	XM_014144418.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47152	XM_014144419.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47153	XM_014144420.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47154	XM_014144421.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47155	XM_014144423.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47156	XM_014144424.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47157	XM_014144425.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47158	XM_014144426.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47159	XM_014144427.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47160	XM_014144428.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47161	XM_014144429.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47162	XM_014144430.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAt47163	XM_014144431.1 Latest version!	Salmo salar afadin-like (LOC106571391), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

LOC106571391 ( XM_014144417.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999892.1 Salmo salar afadin-like (LOC106571391), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAt47150
Clone ID Related Accession (Same CDS sequence)	XM_014144417.1
Accession Version	XM_014144417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGC AGACTGCTGT CCCAGCAATC 
TCTGTTCTAG ACTTGTTGCA GGACGAGGAG CGGCGGAGGA AGCAACAGCT GGAGGAGATC 
CGTAAGCGCG AAGCGGAGGA CCGGGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGGGCCA GACGAGAGGT CGATGAGAAG AGGAGACAGG AGGAGGAATA CTACACACGC 
CTGGAGGAAG GGCGGCGCCG GCAGCACGAG GAGGCCGAGC GCAAGCTGCT GACGCCTGAC 
GAGCCTGGTC TGTACAGACC TCCCCTCCCC AGGGACTACC AGCCTCAGCC CCCCCCTCCC 
ACTGCATCCC CCGCAACCAC TGCCACTAAC GCTCCACCAC CCCCGCCTCA GAGAAACACT 
TCCTACCTCA AAACCCAGGT CGTCTCCCCT GACGCCTTGT ACACTGCCAA GTTTGTCTCG 
TACAATGACG AGGAGGATGA GGACACTACA GGCCTAGCAG GTCAGGTTAA GCTCTCCTCC 
ACCAGGAAGT CCTATGGCGA CCTCCCGCCC GCGCACAAGC CGCAGCCGCC TGCGCGCAAG 
CCCCGTCCTA TGTCTGATGG GATCTTTCTG TCCAGCTCAT TCCAGCCGCC GGCTGTCAAT 
GCCAACAGTA CTGCAGGGCA GCCCCCTCCC CCTCCCATCA AACCCAGCCT CATCCCGACA 
GGCAACTTTA AAGGATTTAA TTCATACACT GGAAACACAA CAGGGGTTGT AGTATCAGGA 
GAAGTTTACA AGGACCTGCG AGAAAAGTGG ACGAAAGGTG GTCAAGAACA GGAAAACACT 
TTAACTGAAG GAGCCCCGGA GAGTATGACA TTCAAGGAGC GCCAGCGGCT CTTTTCCCAA 
GGGAAGGAAG TATCCAACAA AGTCAAGGCC TCGCGAAAAC TCATGGAGCT GGAAAATGAA 
CTCAATACAA AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999892.1
CDS	234..6131
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQEIPEADQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGLYRPPLPRDYQPQPPPPTASPATTATNAPPPPPQRNTSYLKTQVVSPDALYTAKFVSYNDEEDEDTTGLAGQVKLSSTRKSYGDLPPAHKPQPPARKPRPMSDGIFLSSSFQPPAVNANSTAGQPPPPPIKPSLIPTGNFKGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144417.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144417.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGC AGACTGCTGT CCCAGCAATC 
TCTGTTCTAG ACTTGTTGCA GGACGAGGAG CGGCGGAGGA AGCAACAGCT GGAGGAGATC 
CGTAAGCGCG AAGCGGAGGA CCGGGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGGGCCA GACGAGAGGT CGATGAGAAG AGGAGACAGG AGGAGGAATA CTACACACGC 
CTGGAGGAAG GGCGGCGCCG GCAGCACGAG GAGGCCGAGC GCAAGCTGCT GACGCCTGAC 
GAGCCTGGTC TGTACAGACC TCCCCTCCCC AGGGACTACC AGCCTCAGCC CCCCCCTCCC 
ACTGCATCCC CCGCAACCAC TGCCACTAAC GCTCCACCAC CCCCGCCTCA GAGAAACACT 
TCCTACCTCA AAACCCAGGT CGTCTCCCCT GACGCCTTGT ACACTGCCAA GTTTGTCTCG 
TACAATGACG AGGAGGATGA GGACACTACA GGCCTAGCAG GTCAGGTTAA GCTCTCCTCC 
ACCAGGAAGT CCTATGGCGA CCTCCCGCCC GCGCACAAGC CGCAGCCGCC TGCGCGCAAG 
CCCCGTCCTA TGTCTGATGG GATCTTTCTG TCCAGCTCAT TCCAGCCGCC GGCTGTCAAT 
GCCAACAGTA CTGCAGGGCA GCCCCCTCCC CCTCCCATCA AACCCAGCCT CATCCCGACA 
GGCAACTTTA AAGGATTTAA TTCATACACT GGAAACACAA CAGGGGTTGT AGTATCAGGA 
GAAGTTTACA AGGACCTGCG AGAAAAGTGG ACGAAAGGTG GTCAAGAACA GGAAAACACT 
TTAACTGAAG GAGCCCCGGA GAGTATGACA TTCAAGGAGC GCCAGCGGCT CTTTTCCCAA 
GGGAAGGAAG TATCCAACAA AGTCAAGGCC TCGCGAAAAC TCATGGAGCT GGAAAATGAA 
CTCAATACAA AGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144418.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999893.1 Salmo salar afadin-like (LOC106571391), transcript variant X2, mRNA.

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CloneID	OAt47151
Clone ID Related Accession (Same CDS sequence)	XM_014144418.1
Accession Version	XM_014144418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGA CTGCTGTCCC AGCAATCTCT 
GTTCTAGACT TGTTGCAGGA CGAGGAGCGG CGGAGGAAGC AACAGCTGGA GGAGATCCGT 
AAGCGCGAAG CGGAGGACCG GGCCAAACAG GAGGAGGAGA GACGCTGGAG AGAGGAGGAG 
AGGGCCAGAC GAGAGGTCGA TGAGAAGAGG AGACAGGAGG AGGAATACTA CACACGCCTG 
GAGGAAGGGC GGCGCCGGCA GCACGAGGAG GCCGAGCGCA AGCTGCTGAC GCCTGACGAG 
CCTGGTCTGT ACAGACCTCC CCTCCCCAGG GACTACCAGC CTCAGCCCCC CCCTCCCACT 
GCATCCCCCG CAACCACTGC CACTAACGCT CCACCACCCC CGCCTCAGAG AAACACTTCC 
TACCTCAAAA CCCAGGTCGT CTCCCCTGAC GCCTTGTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGATGAGGA CACTACAGGC CTAGCAGGTC AGGTTAAGCT CTCCTCCACC 
AGGAAGTCCT ATGGCGACCT CCCGCCCGCG CACAAGCCGC AGCCGCCTGC GCGCAAGCCC 
CGTCCTATGT CTGATGGGAT CTTTCTGTCC AGCTCATTCC AGCCGCCGGC TGTCAATGCC 
AACAGTACTG CAGGGCAGCC CCCTCCCCCT CCCATCAAAC CCAGCCTCAT CCCGACAGGC 
AACTTTAAAG GATTTAATTC ATACACTGGA AACACAACAG GGGTTGTAGT ATCAGGAGAA 
GTTTACAAGG ACCTGCGAGA AAAGTGGACG AAAGGTGGTC AAGAACAGGA AAACACTTTA 
ACTGAAGGAG CCCCGGAGAG TATGACATTC AAGGAGCGCC AGCGGCTCTT TTCCCAAGGG 
AAGGAAGTAT CCAACAAAGT CAAGGCCTCG CGAAAACTCA TGGAGCTGGA AAATGAACTC 
AATACAAAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999893.1
CDS	234..6128
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQEIPEADQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARTAVPAISVLDLLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGLYRPPLPRDYQPQPPPPTASPATTATNAPPPPPQRNTSYLKTQVVSPDALYTAKFVSYNDEEDEDTTGLAGQVKLSSTRKSYGDLPPAHKPQPPARKPRPMSDGIFLSSSFQPPAVNANSTAGQPPPPPIKPSLIPTGNFKGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144418.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144418.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGA CTGCTGTCCC AGCAATCTCT 
GTTCTAGACT TGTTGCAGGA CGAGGAGCGG CGGAGGAAGC AACAGCTGGA GGAGATCCGT 
AAGCGCGAAG CGGAGGACCG GGCCAAACAG GAGGAGGAGA GACGCTGGAG AGAGGAGGAG 
AGGGCCAGAC GAGAGGTCGA TGAGAAGAGG AGACAGGAGG AGGAATACTA CACACGCCTG 
GAGGAAGGGC GGCGCCGGCA GCACGAGGAG GCCGAGCGCA AGCTGCTGAC GCCTGACGAG 
CCTGGTCTGT ACAGACCTCC CCTCCCCAGG GACTACCAGC CTCAGCCCCC CCCTCCCACT 
GCATCCCCCG CAACCACTGC CACTAACGCT CCACCACCCC CGCCTCAGAG AAACACTTCC 
TACCTCAAAA CCCAGGTCGT CTCCCCTGAC GCCTTGTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGATGAGGA CACTACAGGC CTAGCAGGTC AGGTTAAGCT CTCCTCCACC 
AGGAAGTCCT ATGGCGACCT CCCGCCCGCG CACAAGCCGC AGCCGCCTGC GCGCAAGCCC 
CGTCCTATGT CTGATGGGAT CTTTCTGTCC AGCTCATTCC AGCCGCCGGC TGTCAATGCC 
AACAGTACTG CAGGGCAGCC CCCTCCCCCT CCCATCAAAC CCAGCCTCAT CCCGACAGGC 
AACTTTAAAG GATTTAATTC ATACACTGGA AACACAACAG GGGTTGTAGT ATCAGGAGAA 
GTTTACAAGG ACCTGCGAGA AAAGTGGACG AAAGGTGGTC AAGAACAGGA AAACACTTTA 
ACTGAAGGAG CCCCGGAGAG TATGACATTC AAGGAGCGCC AGCGGCTCTT TTCCCAAGGG 
AAGGAAGTAT CCAACAAAGT CAAGGCCTCG CGAAAACTCA TGGAGCTGGA AAATGAACTC 
AATACAAAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144419.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999894.1 Salmo salar afadin-like (LOC106571391), transcript variant X3, mRNA.

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CloneID	OAt47152
Clone ID Related Accession (Same CDS sequence)	XM_014144419.1
Accession Version	XM_014144419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
CGGAAGCTGG ACCTGGATGA GAAGCCACTG GTGGTGCAGC TCAACTGGAA CAAAGACGAC 
CGCGAGGGAC GTTTCGTCCT CAAGAATGAG AACGACATCA TCCCCAAGAA GGCGCAGAGC 
AACGGGCCCG AGAGGGAGAA GGAGGGAGTG ATCCAGAACT TCAAACGGAC ACTCTCCAAG 
AAAGAGAAGA AGAAGGAGAA GAAGCGAGAG AAGGAGGCGC ACATTGCTGA CGGAGATGAG 
CAAGCACTGA GCAGAGAAGA TGGTGAGAAC TCCAGACTGG CAGCGGAGGT GTACAAGGAC 
ATGCCAGAGA ACAGCTTCAC CAGGACCATC TCCAACCCTG AGGTGGTGAT GAAGAGGAGG 
CGGCAGCAGA AACTGGAGAA GCGGATGCAG GAGTTCCGCA GCAGCGACGG CAGGCCAGAC 
TCGGGGGGGA CGTTGAGGAT CTATGCAGAC AGTCTGAAAC CCAATATCCC CTATAAGACC 
ATCTTGCTGT CCACGACAGA CATGGCAGAT TTTGCTGTGG TGGAGGCCCT GGAGAAGTAC 
GGCCTGGAGA AGGAGAACCC CCGGGAGTAC TGCATCGCCA GGCACACAGA TGACAAGTCT 
GGGAAGGAGA TCATCCTGGA CGATACAGAG TGTCCTCTTC AGATCTTCAG AGACTGGCCC 
AACGACAGAG GGGACCTAGT GTTCCAGCTG AAGAAGAGAC CTCCAGACTA CGTGCCAAAG 
AAGGGCCGCA AGCCAGAGGA CAAGGGCCCC AGGGGGAAGG GCATGGACGT GCCCCTGCAT 
GGGTCTCTAC CCCCAGAGAA ACTGCCTTAC CTGGTGGAGC TCAGCCCAGG GAGGGGGAAT 
CACTATGGCT ACTACACCTA TCGGCACCAC GAAGACGGCT CAGACTCCAG GGACAAACCG 
AAGCTCTACC GTCTGCAGCA CAGTGTGACT GAGGTGGGCT CAGACACGAC AGATGACGGC 
TCCATCCAGC TGTTTGGTCC AGGTATCCTG CCCCACCACT GTAAACTGAT GCACACGGAG 
GGTTTGGTGA CAGTGACACC ACACAGTCTG GAGGCTGAGA CCTACGTGGA CGGCCAGCGT 
GTCGCCGAGA CCACCGTGCT GCGAAGCGGA GCCACACTGC AGTTCGGAGC CTCCCATGTC 
TTCAAGTTTG TGGACCCCAG CTACGACCAC AGCATGGGCA AGGGAGGGCC CGGACCAATG 
ATGAGAGGAA GGCACAAGTC AGGAAGTGTT CCTGAGGCGA CCTTTGACCT GCAAGGGGAC 
CTCCACAGTG GGACTGCCCT CTCCACCAGC AAGAGCTCAG GGAAGCTGGA AATGGAGCGT 
TCATCCAACG GCAGCGAGAG GGGCATGGTC AAACCCATGA TCCGCGGGGA GCAGCAGGAC 
AGCAGGTCCC AGGACAGCCT GAGCCCAGAA GTGACGCTGC CGGCCAGCAT CGAGTTCAGG 
GACAACTCTG AAGATACCTT CCTGACGGCC ATCATCAACT ACACCAACAG CTCCACGGTG 
CACTTCAAGC TGTCCCCCAC CTACGTACTG TACATGACCT GTCGCTACGT CATGTCCAGC 
CACTACAGGC CCGACATCGG TCCCTCGGAG CGCACCCACA AGGTCATAGC CATCGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTC CTGAAGCGGA CCAGAAGCAG 
AAGAACATTG CGGGGTCCCT GGCTTTCTGG ATGGCCAATG CCTCAGAGCT GCTCAACTTC 
ATCAAGCAGG ACAAGGACCT GTCGCGCATC ACACTGGACG CCCAGGACGT GCTGGCACAC 
CTCGTCCAGA TGGCCTTCAA GTACCTGGTC CAGTGTCTGC AGTCTGACCT GAGTAACTAC 
ATGCCTGCCT TCCTGGACGA CCCAGAGGAA CAGAACCCTC AAAGGCCCAA AATAGAGGAT 
GTTTTGCACA CCCTGACGGG CGCCATGTCG CTGCTGCGTC GTTGCCGTGT CAACGCGGCG 
CTGACCATCC AGCTGTTCTC CCAGCTCTTT CACTTCATCA ACATGTGGCT GTTCAACAAG 
CTGGTGATGG AGGCTGACTC TGGACTGTGT TCACACTACT GGGGCGCCAT CCTCCGCCAG 
CAGCTCAGCC ACATCGAGGC CTGGGCAGAG AAACAGGGCC TGGAGCTGGC CGCCGACTGC 
CACCTCAGTC GCATCGTACA GGCTACGACT CTCCTCACCA TGGACAAGTA CTCCATGCAG 
GACGTGCAGA ACATCCACAA CACCTGCTTC AAGCTGAACT CCCTACAGCT CAACGCCCTC 
ATGACCAACT ACCACTGTGC TCCCGACGAG CCTTACATCC CCCCAGAGCT CATAGACCAC 
GTGGTTGCCG TGGCGGAGAA CACAGCAGAT GAGCTGGCCC GTAGCGACGG CCGAGAGGTC 
CAGCTAGAGG AGGACCCTGA CCTGCAGCTG CCCTTCCTAC TGCCCGAGGA CGGCTACTCC 
TGCGACGTCG TGAGGAACAT TCCCAACGGA TTCCAGGAGT TCCTGGACCC ACTTTGTCAG 
AGAGGATTCT GTCGGCTAAC CCCTCACCCG CGCTCCCCTG GGACCTGGAC CATCCACTTT 
GAAGGAGTCG ACTGCGACAG CCACTTCTCT GCAGACAACT CAGACCTGCA AATGCGGAAG 
GAACCGGAAG TTGTGACGGT CACCCTGAAG AAGCACAATG GCATGGGCCT CAGCATTGTG 
GCTGCCAAGG GTGCTGGGCA GGAGAAGCTC GGGATCTACA TCAAGTCTGT TGTGAAGGGA 
GGCGCGGCTG ACGTGGACGG GCGTCTGGCT GCAGGGGACC AGCTGCTGAG TGTGGACGGC 
CGCAGCCTGG TGGGCCTCTC CCAGGAGAGG GCTGCGGAGT TGATGACGAG GACAGGTTCT 
GTGGTAACAC TGGAGGTGGC TAAACAGGGA GCGATCTACC ACGGCCTGGC CACGCTCCTC 
AACCAGCCAT CTCCTATGAT GCCCAGAGCG TCGCAAGGAG GGCGTGACAA GAATGGCAAA 
CTGAGACCAA AGAGCGAAGG CTTTGAACTG TACAACAACT CGGTCCAGAA CGGCTCTCCA 
GAGAGCCCCC AGGCCGACTG GCAGGCCTAC CCAGAACCAA AGAAGATGAG CGGGGAGGAG 
AGGCTCCTGA AGAACCGGGC CGACCACCGC TCCAGCCCCA ACGTGGCCAA CCAGAGCCAG 
AGTCCGGGAG GGAAGGCCGT CTACACACGA GGACCTGGGA CCAAGATCAC CTCTGTCTCC 
ACGGGCAACC TGTGTGTTGA TGACGAGCCC TCTCCTCCCC ATCCGGAGGC CTATCCTATT 
CCCACTCAGA CCTACCCCAG GGAATACTTC ACCATCCCAG CCTCCAAGTC TACGAACCGG 
ATGGACCCGG CCCCAGGACA GTTATACGCT GACGACAGGG GTCCTCCCGG CCCCATGGAA 
GACCACCACC ATAACAGCAT AGCTATGCAG CGGGTGGACC ACTCTCAGGA GGAGCTGTGT 
GACCTCCCGG GGGGCTACCC TCCAACCAGC CTGCGTCCTG AGGACCTGGT CCACTTGCAC 
CAGCAGCAGC AGAGAGAGGC TGACTACCAG TGGGGCAGTG GGGAACACTG GGTCAACCAC 
CACCAGGTCT CCTCTTCCGT GGAGTCCTCC ACCTCCAGCC AGGAGCACCT CAACTACTCC 
TCGTCCTCCT CCAACAAGAC CCAGAGCAAC CACAAGAGTG GCCCCGGACG CTGGAAGACC 
CCCGGCCCCA TTGCCCCCCA CCCAGGCCCC GTGGCCCAGT CCACACGCAC GGACCTCCTT 
CCCCCTCCAC CGCCCCCTCC CCCCGTACAC TACCAGGACT TTGAGCTCCA GCCCGACCTG 
CCCCTGCCCC CTCCTCCTTC GGTCACCCAG CAGCAGGCCG CAGCAGCAGC CGCCGACCGC 
AAGAAGCGCG AGGAGCAGCA GCGCTGGTAT GAGAAGGAGA AGGCCCGGTT AGAGGAGGAG 
AGGGAGAGGA AGAGGAGGGA GCAGGAGAGG AAGCTGGGAC AGGTCCGTAA CCCAGCTGTG 
CCTGTAACCC ACAACAACAA CCAACACGGG GGCACACTCC CCCCTCCCAC ACAGCACCAG 
CAGGTGCCCC CTCCCCGGTC CCCCATGCAG CAGGTGCCCC CTCCCCGGTC CCCCATGCAG 
CAAGTGCCCC ATCCCTACCT CCCCCCCCAG GGCCAACAGC AGGCCCAGCA GCAGCAGCCC 
CCCAGGCCAG AGAAACTGTC CACCCTGCCC AGAGCGGGCA CCCTGCCCCC TCAGGGTAAC 
CCCATGGACA CAGTGATCAG AGAGCTGCTC CCCCAGCAGC AGCCCCGCAC CATCGAGCGC 
CGGGACCTGC AGTACATCAC CATCAGCAAG GAGGAGCTGT CCTCCAGCGA CAGCCTGTCC 
CCAGACCCCT GGAAGAGGGA TGCCCGGGAG AAGTTGGAGA AGCAGCAGCA GCTGCACATC 
GTAGACCTGC TGGACAGGGA GATCCAGGAC CTGCAGGCCA AGCCGGAGCG TACGGCCGAG 
GAGAGCGACC GGCTCAGGAA GTTGATGTTG GAGTGGCAGT TCCAGAAGAG ACTGCAGGAG 
TCCAAGCACA GTGATGAGGA AGATGAGGAG GATGACGACG AGGACGTGGA CACCATGATG 
ATCATGCAGC GGCTGGAGGC AGAGAAGAGA GCAAGGCAGA CTGCTGTCCC AGCAATCTCT 
GTTCTAGACT TGTTGCAGGA CGAGGAGCGG CGGAGGAAGC AACAGCTGGA GGAGATCCGT 
AAGCGCGAAG CGGAGGACCG GGCCAAACAG GAGGAGGAGA GACGCTGGAG AGAGGAGGAG 
AGGGCCAGAC GAGAGGTCGA TGAGAAGAGG AGACAGGAGG AGGAATACTA CACACGCCTG 
GAGGAAGGGC GGCGCCGGCA GCACGAGGAG GCCGAGCGCA AGCTGCTGAC GCCTGACGAG 
CCTGGTCTGT ACAGACCTCC CCTCCCCAGG GACTACCAGC CTCAGCCCCC CCCTCCCACT 
GCATCCCCCG CAACCACTGC CACTAACGCT CCACCACCCC CGCCTCAGAG AAACACTTCC 
TACCTCAAAA CCCAGGTCGT CTCCCCTGAC GCCTTGTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGATGAGGA CACTACAGGC CTAGCAGGTC AGGTTAAGCT CTCCTCCACC 
AGGAAGTCCT ATGGCGACCT CCCGCCCGCG CACAAGCCGC AGCCGCCTGC GCGCAAGCCC 
CGTCCTATGT CTGATGGGAT CTTTCTGTCC AGCTCATTCC AGCCGCCGGC TGTCAATGCC 
AACAGTACTG CAGGGCAGCC CCCTCCCCCT CCCATCAAAC CCAGCCTCAT CCCGACAGGC 
AACTTTAAAG GATTTAATTC ATACACTGGA AACACAACAG GGGTTGTAGT ATCAGGAGAA 
GTTTACAAGG ACCTGCGAGA AAAGTGGACG AAAGGTGGTC AAGAACAGGA AAACACTTTA 
ACTGAAGGAG CCCCGGAGAG TATGACATTC AAGGAGCGCC AGCGGCTCTT TTCCCAAGGG 
AAGGAAGTAT CCAACAAAGT CAAGGCCTCG CGAAAACTCA TGGAGCTGGA AAATGAACTC 
AATACAAAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999894.1
CDS	234..6128
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQEIPEADQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGLYRPPLPRDYQPQPPPPTASPATTATNAPPPPPQRNTSYLKTQVVSPDALYTAKFVSYNDEEDEDTTGLAGQVKLSSTRKSYGDLPPAHKPQPPARKPRPMSDGIFLSSSFQPPAVNANSTAGQPPPPPIKPSLIPTGNFKGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144419.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144419.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
CGGAAGCTGG ACCTGGATGA GAAGCCACTG GTGGTGCAGC TCAACTGGAA CAAAGACGAC 
CGCGAGGGAC GTTTCGTCCT CAAGAATGAG AACGACATCA TCCCCAAGAA GGCGCAGAGC 
AACGGGCCCG AGAGGGAGAA GGAGGGAGTG ATCCAGAACT TCAAACGGAC ACTCTCCAAG 
AAAGAGAAGA AGAAGGAGAA GAAGCGAGAG AAGGAGGCGC ACATTGCTGA CGGAGATGAG 
CAAGCACTGA GCAGAGAAGA TGGTGAGAAC TCCAGACTGG CAGCGGAGGT GTACAAGGAC 
ATGCCAGAGA ACAGCTTCAC CAGGACCATC TCCAACCCTG AGGTGGTGAT GAAGAGGAGG 
CGGCAGCAGA AACTGGAGAA GCGGATGCAG GAGTTCCGCA GCAGCGACGG CAGGCCAGAC 
TCGGGGGGGA CGTTGAGGAT CTATGCAGAC AGTCTGAAAC CCAATATCCC CTATAAGACC 
ATCTTGCTGT CCACGACAGA CATGGCAGAT TTTGCTGTGG TGGAGGCCCT GGAGAAGTAC 
GGCCTGGAGA AGGAGAACCC CCGGGAGTAC TGCATCGCCA GGCACACAGA TGACAAGTCT 
GGGAAGGAGA TCATCCTGGA CGATACAGAG TGTCCTCTTC AGATCTTCAG AGACTGGCCC 
AACGACAGAG GGGACCTAGT GTTCCAGCTG AAGAAGAGAC CTCCAGACTA CGTGCCAAAG 
AAGGGCCGCA AGCCAGAGGA CAAGGGCCCC AGGGGGAAGG GCATGGACGT GCCCCTGCAT 
GGGTCTCTAC CCCCAGAGAA ACTGCCTTAC CTGGTGGAGC TCAGCCCAGG GAGGGGGAAT 
CACTATGGCT ACTACACCTA TCGGCACCAC GAAGACGGCT CAGACTCCAG GGACAAACCG 
AAGCTCTACC GTCTGCAGCA CAGTGTGACT GAGGTGGGCT CAGACACGAC AGATGACGGC 
TCCATCCAGC TGTTTGGTCC AGGTATCCTG CCCCACCACT GTAAACTGAT GCACACGGAG 
GGTTTGGTGA CAGTGACACC ACACAGTCTG GAGGCTGAGA CCTACGTGGA CGGCCAGCGT 
GTCGCCGAGA CCACCGTGCT GCGAAGCGGA GCCACACTGC AGTTCGGAGC CTCCCATGTC 
TTCAAGTTTG TGGACCCCAG CTACGACCAC AGCATGGGCA AGGGAGGGCC CGGACCAATG 
ATGAGAGGAA GGCACAAGTC AGGAAGTGTT CCTGAGGCGA CCTTTGACCT GCAAGGGGAC 
CTCCACAGTG GGACTGCCCT CTCCACCAGC AAGAGCTCAG GGAAGCTGGA AATGGAGCGT 
TCATCCAACG GCAGCGAGAG GGGCATGGTC AAACCCATGA TCCGCGGGGA GCAGCAGGAC 
AGCAGGTCCC AGGACAGCCT GAGCCCAGAA GTGACGCTGC CGGCCAGCAT CGAGTTCAGG 
GACAACTCTG AAGATACCTT CCTGACGGCC ATCATCAACT ACACCAACAG CTCCACGGTG 
CACTTCAAGC TGTCCCCCAC CTACGTACTG TACATGACCT GTCGCTACGT CATGTCCAGC 
CACTACAGGC CCGACATCGG TCCCTCGGAG CGCACCCACA AGGTCATAGC CATCGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGGAGATTC CTGAAGCGGA CCAGAAGCAG 
AAGAACATTG CGGGGTCCCT GGCTTTCTGG ATGGCCAATG CCTCAGAGCT GCTCAACTTC 
ATCAAGCAGG ACAAGGACCT GTCGCGCATC ACACTGGACG CCCAGGACGT GCTGGCACAC 
CTCGTCCAGA TGGCCTTCAA GTACCTGGTC CAGTGTCTGC AGTCTGACCT GAGTAACTAC 
ATGCCTGCCT TCCTGGACGA CCCAGAGGAA CAGAACCCTC AAAGGCCCAA AATAGAGGAT 
GTTTTGCACA CCCTGACGGG CGCCATGTCG CTGCTGCGTC GTTGCCGTGT CAACGCGGCG 
CTGACCATCC AGCTGTTCTC CCAGCTCTTT CACTTCATCA ACATGTGGCT GTTCAACAAG 
CTGGTGATGG AGGCTGACTC TGGACTGTGT TCACACTACT GGGGCGCCAT CCTCCGCCAG 
CAGCTCAGCC ACATCGAGGC CTGGGCAGAG AAACAGGGCC TGGAGCTGGC CGCCGACTGC 
CACCTCAGTC GCATCGTACA GGCTACGACT CTCCTCACCA TGGACAAGTA CTCCATGCAG 
GACGTGCAGA ACATCCACAA CACCTGCTTC AAGCTGAACT CCCTACAGCT CAACGCCCTC 
ATGACCAACT ACCACTGTGC TCCCGACGAG CCTTACATCC CCCCAGAGCT CATAGACCAC 
GTGGTTGCCG TGGCGGAGAA CACAGCAGAT GAGCTGGCCC GTAGCGACGG CCGAGAGGTC 
CAGCTAGAGG AGGACCCTGA CCTGCAGCTG CCCTTCCTAC TGCCCGAGGA CGGCTACTCC 
TGCGACGTCG TGAGGAACAT TCCCAACGGA TTCCAGGAGT TCCTGGACCC ACTTTGTCAG 
AGAGGATTCT GTCGGCTAAC CCCTCACCCG CGCTCCCCTG GGACCTGGAC CATCCACTTT 
GAAGGAGTCG ACTGCGACAG CCACTTCTCT GCAGACAACT CAGACCTGCA AATGCGGAAG 
GAACCGGAAG TTGTGACGGT CACCCTGAAG AAGCACAATG GCATGGGCCT CAGCATTGTG 
GCTGCCAAGG GTGCTGGGCA GGAGAAGCTC GGGATCTACA TCAAGTCTGT TGTGAAGGGA 
GGCGCGGCTG ACGTGGACGG GCGTCTGGCT GCAGGGGACC AGCTGCTGAG TGTGGACGGC 
CGCAGCCTGG TGGGCCTCTC CCAGGAGAGG GCTGCGGAGT TGATGACGAG GACAGGTTCT 
GTGGTAACAC TGGAGGTGGC TAAACAGGGA GCGATCTACC ACGGCCTGGC CACGCTCCTC 
AACCAGCCAT CTCCTATGAT GCCCAGAGCG TCGCAAGGAG GGCGTGACAA GAATGGCAAA 
CTGAGACCAA AGAGCGAAGG CTTTGAACTG TACAACAACT CGGTCCAGAA CGGCTCTCCA 
GAGAGCCCCC AGGCCGACTG GCAGGCCTAC CCAGAACCAA AGAAGATGAG CGGGGAGGAG 
AGGCTCCTGA AGAACCGGGC CGACCACCGC TCCAGCCCCA ACGTGGCCAA CCAGAGCCAG 
AGTCCGGGAG GGAAGGCCGT CTACACACGA GGACCTGGGA CCAAGATCAC CTCTGTCTCC 
ACGGGCAACC TGTGTGTTGA TGACGAGCCC TCTCCTCCCC ATCCGGAGGC CTATCCTATT 
CCCACTCAGA CCTACCCCAG GGAATACTTC ACCATCCCAG CCTCCAAGTC TACGAACCGG 
ATGGACCCGG CCCCAGGACA GTTATACGCT GACGACAGGG GTCCTCCCGG CCCCATGGAA 
GACCACCACC ATAACAGCAT AGCTATGCAG CGGGTGGACC ACTCTCAGGA GGAGCTGTGT 
GACCTCCCGG GGGGCTACCC TCCAACCAGC CTGCGTCCTG AGGACCTGGT CCACTTGCAC 
CAGCAGCAGC AGAGAGAGGC TGACTACCAG TGGGGCAGTG GGGAACACTG GGTCAACCAC 
CACCAGGTCT CCTCTTCCGT GGAGTCCTCC ACCTCCAGCC AGGAGCACCT CAACTACTCC 
TCGTCCTCCT CCAACAAGAC CCAGAGCAAC CACAAGAGTG GCCCCGGACG CTGGAAGACC 
CCCGGCCCCA TTGCCCCCCA CCCAGGCCCC GTGGCCCAGT CCACACGCAC GGACCTCCTT 
CCCCCTCCAC CGCCCCCTCC CCCCGTACAC TACCAGGACT TTGAGCTCCA GCCCGACCTG 
CCCCTGCCCC CTCCTCCTTC GGTCACCCAG CAGCAGGCCG CAGCAGCAGC CGCCGACCGC 
AAGAAGCGCG AGGAGCAGCA GCGCTGGTAT GAGAAGGAGA AGGCCCGGTT AGAGGAGGAG 
AGGGAGAGGA AGAGGAGGGA GCAGGAGAGG AAGCTGGGAC AGGTCCGTAA CCCAGCTGTG 
CCTGTAACCC ACAACAACAA CCAACACGGG GGCACACTCC CCCCTCCCAC ACAGCACCAG 
CAGGTGCCCC CTCCCCGGTC CCCCATGCAG CAGGTGCCCC CTCCCCGGTC CCCCATGCAG 
CAAGTGCCCC ATCCCTACCT CCCCCCCCAG GGCCAACAGC AGGCCCAGCA GCAGCAGCCC 
CCCAGGCCAG AGAAACTGTC CACCCTGCCC AGAGCGGGCA CCCTGCCCCC TCAGGGTAAC 
CCCATGGACA CAGTGATCAG AGAGCTGCTC CCCCAGCAGC AGCCCCGCAC CATCGAGCGC 
CGGGACCTGC AGTACATCAC CATCAGCAAG GAGGAGCTGT CCTCCAGCGA CAGCCTGTCC 
CCAGACCCCT GGAAGAGGGA TGCCCGGGAG AAGTTGGAGA AGCAGCAGCA GCTGCACATC 
GTAGACCTGC TGGACAGGGA GATCCAGGAC CTGCAGGCCA AGCCGGAGCG TACGGCCGAG 
GAGAGCGACC GGCTCAGGAA GTTGATGTTG GAGTGGCAGT TCCAGAAGAG ACTGCAGGAG 
TCCAAGCACA GTGATGAGGA AGATGAGGAG GATGACGACG AGGACGTGGA CACCATGATG 
ATCATGCAGC GGCTGGAGGC AGAGAAGAGA GCAAGGCAGA CTGCTGTCCC AGCAATCTCT 
GTTCTAGACT TGTTGCAGGA CGAGGAGCGG CGGAGGAAGC AACAGCTGGA GGAGATCCGT 
AAGCGCGAAG CGGAGGACCG GGCCAAACAG GAGGAGGAGA GACGCTGGAG AGAGGAGGAG 
AGGGCCAGAC GAGAGGTCGA TGAGAAGAGG AGACAGGAGG AGGAATACTA CACACGCCTG 
GAGGAAGGGC GGCGCCGGCA GCACGAGGAG GCCGAGCGCA AGCTGCTGAC GCCTGACGAG 
CCTGGTCTGT ACAGACCTCC CCTCCCCAGG GACTACCAGC CTCAGCCCCC CCCTCCCACT 
GCATCCCCCG CAACCACTGC CACTAACGCT CCACCACCCC CGCCTCAGAG AAACACTTCC 
TACCTCAAAA CCCAGGTCGT CTCCCCTGAC GCCTTGTACA CTGCCAAGTT TGTCTCGTAC 
AATGACGAGG AGGATGAGGA CACTACAGGC CTAGCAGGTC AGGTTAAGCT CTCCTCCACC 
AGGAAGTCCT ATGGCGACCT CCCGCCCGCG CACAAGCCGC AGCCGCCTGC GCGCAAGCCC 
CGTCCTATGT CTGATGGGAT CTTTCTGTCC AGCTCATTCC AGCCGCCGGC TGTCAATGCC 
AACAGTACTG CAGGGCAGCC CCCTCCCCCT CCCATCAAAC CCAGCCTCAT CCCGACAGGC 
AACTTTAAAG GATTTAATTC ATACACTGGA AACACAACAG GGGTTGTAGT ATCAGGAGAA 
GTTTACAAGG ACCTGCGAGA AAAGTGGACG AAAGGTGGTC AAGAACAGGA AAACACTTTA 
ACTGAAGGAG CCCCGGAGAG TATGACATTC AAGGAGCGCC AGCGGCTCTT TTCCCAAGGG 
AAGGAAGTAT CCAACAAAGT CAAGGCCTCG CGAAAACTCA TGGAGCTGGA AAATGAACTC 
AATACAAAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144420.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999895.1 Salmo salar afadin-like (LOC106571391), transcript variant X4, mRNA.

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CloneID	OAt47153
Clone ID Related Accession (Same CDS sequence)	XM_014144420.1
Accession Version	XM_014144420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGAAGC AGAAGAACAT TGCGGGGTCC 
CTGGCTTTCT GGATGGCCAA TGCCTCAGAG CTGCTCAACT TCATCAAGCA GGACAAGGAC 
CTGTCGCGCA TCACACTGGA CGCCCAGGAC GTGCTGGCAC ACCTCGTCCA GATGGCCTTC 
AAGTACCTGG TCCAGTGTCT GCAGTCTGAC CTGAGTAACT ACATGCCTGC CTTCCTGGAC 
GACCCAGAGG AACAGAACCC TCAAAGGCCC AAAATAGAGG ATGTTTTGCA CACCCTGACG 
GGCGCCATGT CGCTGCTGCG TCGTTGCCGT GTCAACGCGG CGCTGACCAT CCAGCTGTTC 
TCCCAGCTCT TTCACTTCAT CAACATGTGG CTGTTCAACA AGCTGGTGAT GGAGGCTGAC 
TCTGGACTGT GTTCACACTA CTGGGGCGCC ATCCTCCGCC AGCAGCTCAG CCACATCGAG 
GCCTGGGCAG AGAAACAGGG CCTGGAGCTG GCCGCCGACT GCCACCTCAG TCGCATCGTA 
CAGGCTACGA CTCTCCTCAC CATGGACAAG TACTCCATGC AGGACGTGCA GAACATCCAC 
AACACCTGCT TCAAGCTGAA CTCCCTACAG CTCAACGCCC TCATGACCAA CTACCACTGT 
GCTCCCGACG AGCCTTACAT CCCCCCAGAG CTCATAGACC ACGTGGTTGC CGTGGCGGAG 
AACACAGCAG ATGAGCTGGC CCGTAGCGAC GGCCGAGAGG TCCAGCTAGA GGAGGACCCT 
GACCTGCAGC TGCCCTTCCT ACTGCCCGAG GACGGCTACT CCTGCGACGT CGTGAGGAAC 
ATTCCCAACG GATTCCAGGA GTTCCTGGAC CCACTTTGTC AGAGAGGATT CTGTCGGCTA 
ACCCCTCACC CGCGCTCCCC TGGGACCTGG ACCATCCACT TTGAAGGAGT CGACTGCGAC 
AGCCACTTCT CTGCAGACAA CTCAGACCTG CAAATGCGGA AGGAACCGGA AGTTGTGACG 
GTCACCCTGA AGAAGCACAA TGGCATGGGC CTCAGCATTG TGGCTGCCAA GGGTGCTGGG 
CAGGAGAAGC TCGGGATCTA CATCAAGTCT GTTGTGAAGG GAGGCGCGGC TGACGTGGAC 
GGGCGTCTGG CTGCAGGGGA CCAGCTGCTG AGTGTGGACG GCCGCAGCCT GGTGGGCCTC 
TCCCAGGAGA GGGCTGCGGA GTTGATGACG AGGACAGGTT CTGTGGTAAC ACTGGAGGTG 
GCTAAACAGG GAGCGATCTA CCACGGCCTG GCCACGCTCC TCAACCAGCC ATCTCCTATG 
ATGCCCAGAG CGTCGCAAGG AGGGCGTGAC AAGAATGGCA AACTGAGACC AAAGAGCGAA 
GGCTTTGAAC TGTACAACAA CTCGGTCCAG AACGGCTCTC CAGAGAGCCC CCAGGCCGAC 
TGGCAGGCCT ACCCAGAACC AAAGAAGATG AGCGGGGAGG AGAGGCTCCT GAAGAACCGG 
GCCGACCACC GCTCCAGCCC CAACGTGGCC AACCAGAGCC AGAGTCCGGG AGGGAAGGCC 
GTCTACACAC GAGGACCTGG GACCAAGATC ACCTCTGTCT CCACGGGCAA CCTGTGTGTT 
GATGACGAGC CCTCTCCTCC CCATCCGGAG GCCTATCCTA TTCCCACTCA GACCTACCCC 
AGGGAATACT TCACCATCCC AGCCTCCAAG TCTACGAACC GGATGGACCC GGCCCCAGGA 
CAGTTATACG CTGACGACAG GGGTCCTCCC GGCCCCATGG AAGACCACCA CCATAACAGC 
ATAGCTATGC AGCGGGTGGA CCACTCTCAG GAGGAGCTGT GTGACCTCCC GGGGGGCTAC 
CCTCCAACCA GCCTGCGTCC TGAGGACCTG GTCCACTTGC ACCAGCAGCA GCAGAGAGAG 
GCTGACTACC AGTGGGGCAG TGGGGAACAC TGGGTCAACC ACCACCAGGT CTCCTCTTCC 
GTGGAGTCCT CCACCTCCAG CCAGGAGCAC CTCAACTACT CCTCGTCCTC CTCCAACAAG 
ACCCAGAGCA ACCACAAGAG TGGCCCCGGA CGCTGGAAGA CCCCCGGCCC CATTGCCCCC 
CACCCAGGCC CCGTGGCCCA GTCCACACGC ACGGACCTCC TTCCCCCTCC ACCGCCCCCT 
CCCCCCGTAC ACTACCAGGA CTTTGAGCTC CAGCCCGACC TGCCCCTGCC CCCTCCTCCT 
TCGGTCACCC AGCAGCAGGC CGCAGCAGCA GCCGCCGACC GCAAGAAGCG CGAGGAGCAG 
CAGCGCTGGT ATGAGAAGGA GAAGGCCCGG TTAGAGGAGG AGAGGGAGAG GAAGAGGAGG 
GAGCAGGAGA GGAAGCTGGG ACAGGTCCGT AACCCAGCTG TGCCTGTAAC CCACAACAAC 
AACCAACACG GGGGCACACT CCCCCCTCCC ACACAGCACC AGCAGGTGCC CCCTCCCCGG 
TCCCCCATGC AGCAGGTGCC CCCTCCCCGG TCCCCCATGC AGCAAGTGCC CCATCCCTAC 
CTCCCCCCCC AGGGCCAACA GCAGGCCCAG CAGCAGCAGC CCCCCAGGCC AGAGAAACTG 
TCCACCCTGC CCAGAGCGGG CACCCTGCCC CCTCAGGGTA ACCCCATGGA CACAGTGATC 
AGAGAGCTGC TCCCCCAGCA GCAGCCCCGC ACCATCGAGC GCCGGGACCT GCAGTACATC 
ACCATCAGCA AGGAGGAGCT GTCCTCCAGC GACAGCCTGT CCCCAGACCC CTGGAAGAGG 
GATGCCCGGG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTAGACCT GCTGGACAGG 
GAGATCCAGG ACCTGCAGGC CAAGCCGGAG CGTACGGCCG AGGAGAGCGA CCGGCTCAGG 
AAGTTGATGT TGGAGTGGCA GTTCCAGAAG AGACTGCAGG AGTCCAAGCA CAGTGATGAG 
GAAGATGAGG AGGATGACGA CGAGGACGTG GACACCATGA TGATCATGCA GCGGCTGGAG 
GCAGAGAAGA GAGCAAGGCA GACTGCTGTC CCAGCAATCT CTGTTCTAGA CTTGTTGCAG 
GACGAGGAGC GGCGGAGGAA GCAACAGCTG GAGGAGATCC GTAAGCGCGA AGCGGAGGAC 
CGGGCCAAAC AGGAGGAGGA GAGACGCTGG AGAGAGGAGG AGAGGGCCAG ACGAGAGGTC 
GATGAGAAGA GGAGACAGGA GGAGGAATAC TACACACGCC TGGAGGAAGG GCGGCGCCGG 
CAGCACGAGG AGGCCGAGCG CAAGCTGCTG ACGCCTGACG AGCCTGGTCT GTACAGACCT 
CCCCTCCCCA GGGACTACCA GCCTCAGCCC CCCCCTCCCA CTGCATCCCC CGCAACCACT 
GCCACTAACG CTCCACCACC CCCGCCTCAG AGAAACACTT CCTACCTCAA AACCCAGGTC 
GTCTCCCCTG ACGCCTTGTA CACTGCCAAG TTTGTCTCGT ACAATGACGA GGAGGATGAG 
GACACTACAG GCCTAGCAGG TCAGGTTAAG CTCTCCTCCA CCAGGAAGTC CTATGGCGAC 
CTCCCGCCCG CGCACAAGCC GCAGCCGCCT GCGCGCAAGC CCCGTCCTAT GTCTGATGGG 
ATCTTTCTGT CCAGCTCATT CCAGCCGCCG GCTGTCAATG CCAACAGTAC TGCAGGGCAG 
CCCCCTCCCC CTCCCATCAA ACCCAGCCTC ATCCCGACAG GCAACTTTAA AGGATTTAAT 
TCATACACTG GAAACACAAC AGGGGTTGTA GTATCAGGAG AAGTTTACAA GGACCTGCGA 
GAAAAGTGGA CGAAAGGTGG TCAAGAACAG GAAAACACTT TAACTGAAGG AGCCCCGGAG 
AGTATGACAT TCAAGGAGCG CCAGCGGCTC TTTTCCCAAG GGAAGGAAGT ATCCAACAAA 
GTCAAGGCCT CGCGAAAACT CATGGAGCTG GAAAATGAAC TCAATACAAA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999895.1
CDS	233..6109
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGLYRPPLPRDYQPQPPPPTASPATTATNAPPPPPQRNTSYLKTQVVSPDALYTAKFVSYNDEEDEDTTGLAGQVKLSSTRKSYGDLPPAHKPQPPARKPRPMSDGIFLSSSFQPPAVNANSTAGQPPPPPIKPSLIPTGNFKGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144420.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144420.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGAAGC AGAAGAACAT TGCGGGGTCC 
CTGGCTTTCT GGATGGCCAA TGCCTCAGAG CTGCTCAACT TCATCAAGCA GGACAAGGAC 
CTGTCGCGCA TCACACTGGA CGCCCAGGAC GTGCTGGCAC ACCTCGTCCA GATGGCCTTC 
AAGTACCTGG TCCAGTGTCT GCAGTCTGAC CTGAGTAACT ACATGCCTGC CTTCCTGGAC 
GACCCAGAGG AACAGAACCC TCAAAGGCCC AAAATAGAGG ATGTTTTGCA CACCCTGACG 
GGCGCCATGT CGCTGCTGCG TCGTTGCCGT GTCAACGCGG CGCTGACCAT CCAGCTGTTC 
TCCCAGCTCT TTCACTTCAT CAACATGTGG CTGTTCAACA AGCTGGTGAT GGAGGCTGAC 
TCTGGACTGT GTTCACACTA CTGGGGCGCC ATCCTCCGCC AGCAGCTCAG CCACATCGAG 
GCCTGGGCAG AGAAACAGGG CCTGGAGCTG GCCGCCGACT GCCACCTCAG TCGCATCGTA 
CAGGCTACGA CTCTCCTCAC CATGGACAAG TACTCCATGC AGGACGTGCA GAACATCCAC 
AACACCTGCT TCAAGCTGAA CTCCCTACAG CTCAACGCCC TCATGACCAA CTACCACTGT 
GCTCCCGACG AGCCTTACAT CCCCCCAGAG CTCATAGACC ACGTGGTTGC CGTGGCGGAG 
AACACAGCAG ATGAGCTGGC CCGTAGCGAC GGCCGAGAGG TCCAGCTAGA GGAGGACCCT 
GACCTGCAGC TGCCCTTCCT ACTGCCCGAG GACGGCTACT CCTGCGACGT CGTGAGGAAC 
ATTCCCAACG GATTCCAGGA GTTCCTGGAC CCACTTTGTC AGAGAGGATT CTGTCGGCTA 
ACCCCTCACC CGCGCTCCCC TGGGACCTGG ACCATCCACT TTGAAGGAGT CGACTGCGAC 
AGCCACTTCT CTGCAGACAA CTCAGACCTG CAAATGCGGA AGGAACCGGA AGTTGTGACG 
GTCACCCTGA AGAAGCACAA TGGCATGGGC CTCAGCATTG TGGCTGCCAA GGGTGCTGGG 
CAGGAGAAGC TCGGGATCTA CATCAAGTCT GTTGTGAAGG GAGGCGCGGC TGACGTGGAC 
GGGCGTCTGG CTGCAGGGGA CCAGCTGCTG AGTGTGGACG GCCGCAGCCT GGTGGGCCTC 
TCCCAGGAGA GGGCTGCGGA GTTGATGACG AGGACAGGTT CTGTGGTAAC ACTGGAGGTG 
GCTAAACAGG GAGCGATCTA CCACGGCCTG GCCACGCTCC TCAACCAGCC ATCTCCTATG 
ATGCCCAGAG CGTCGCAAGG AGGGCGTGAC AAGAATGGCA AACTGAGACC AAAGAGCGAA 
GGCTTTGAAC TGTACAACAA CTCGGTCCAG AACGGCTCTC CAGAGAGCCC CCAGGCCGAC 
TGGCAGGCCT ACCCAGAACC AAAGAAGATG AGCGGGGAGG AGAGGCTCCT GAAGAACCGG 
GCCGACCACC GCTCCAGCCC CAACGTGGCC AACCAGAGCC AGAGTCCGGG AGGGAAGGCC 
GTCTACACAC GAGGACCTGG GACCAAGATC ACCTCTGTCT CCACGGGCAA CCTGTGTGTT 
GATGACGAGC CCTCTCCTCC CCATCCGGAG GCCTATCCTA TTCCCACTCA GACCTACCCC 
AGGGAATACT TCACCATCCC AGCCTCCAAG TCTACGAACC GGATGGACCC GGCCCCAGGA 
CAGTTATACG CTGACGACAG GGGTCCTCCC GGCCCCATGG AAGACCACCA CCATAACAGC 
ATAGCTATGC AGCGGGTGGA CCACTCTCAG GAGGAGCTGT GTGACCTCCC GGGGGGCTAC 
CCTCCAACCA GCCTGCGTCC TGAGGACCTG GTCCACTTGC ACCAGCAGCA GCAGAGAGAG 
GCTGACTACC AGTGGGGCAG TGGGGAACAC TGGGTCAACC ACCACCAGGT CTCCTCTTCC 
GTGGAGTCCT CCACCTCCAG CCAGGAGCAC CTCAACTACT CCTCGTCCTC CTCCAACAAG 
ACCCAGAGCA ACCACAAGAG TGGCCCCGGA CGCTGGAAGA CCCCCGGCCC CATTGCCCCC 
CACCCAGGCC CCGTGGCCCA GTCCACACGC ACGGACCTCC TTCCCCCTCC ACCGCCCCCT 
CCCCCCGTAC ACTACCAGGA CTTTGAGCTC CAGCCCGACC TGCCCCTGCC CCCTCCTCCT 
TCGGTCACCC AGCAGCAGGC CGCAGCAGCA GCCGCCGACC GCAAGAAGCG CGAGGAGCAG 
CAGCGCTGGT ATGAGAAGGA GAAGGCCCGG TTAGAGGAGG AGAGGGAGAG GAAGAGGAGG 
GAGCAGGAGA GGAAGCTGGG ACAGGTCCGT AACCCAGCTG TGCCTGTAAC CCACAACAAC 
AACCAACACG GGGGCACACT CCCCCCTCCC ACACAGCACC AGCAGGTGCC CCCTCCCCGG 
TCCCCCATGC AGCAGGTGCC CCCTCCCCGG TCCCCCATGC AGCAAGTGCC CCATCCCTAC 
CTCCCCCCCC AGGGCCAACA GCAGGCCCAG CAGCAGCAGC CCCCCAGGCC AGAGAAACTG 
TCCACCCTGC CCAGAGCGGG CACCCTGCCC CCTCAGGGTA ACCCCATGGA CACAGTGATC 
AGAGAGCTGC TCCCCCAGCA GCAGCCCCGC ACCATCGAGC GCCGGGACCT GCAGTACATC 
ACCATCAGCA AGGAGGAGCT GTCCTCCAGC GACAGCCTGT CCCCAGACCC CTGGAAGAGG 
GATGCCCGGG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTAGACCT GCTGGACAGG 
GAGATCCAGG ACCTGCAGGC CAAGCCGGAG CGTACGGCCG AGGAGAGCGA CCGGCTCAGG 
AAGTTGATGT TGGAGTGGCA GTTCCAGAAG AGACTGCAGG AGTCCAAGCA CAGTGATGAG 
GAAGATGAGG AGGATGACGA CGAGGACGTG GACACCATGA TGATCATGCA GCGGCTGGAG 
GCAGAGAAGA GAGCAAGGCA GACTGCTGTC CCAGCAATCT CTGTTCTAGA CTTGTTGCAG 
GACGAGGAGC GGCGGAGGAA GCAACAGCTG GAGGAGATCC GTAAGCGCGA AGCGGAGGAC 
CGGGCCAAAC AGGAGGAGGA GAGACGCTGG AGAGAGGAGG AGAGGGCCAG ACGAGAGGTC 
GATGAGAAGA GGAGACAGGA GGAGGAATAC TACACACGCC TGGAGGAAGG GCGGCGCCGG 
CAGCACGAGG AGGCCGAGCG CAAGCTGCTG ACGCCTGACG AGCCTGGTCT GTACAGACCT 
CCCCTCCCCA GGGACTACCA GCCTCAGCCC CCCCCTCCCA CTGCATCCCC CGCAACCACT 
GCCACTAACG CTCCACCACC CCCGCCTCAG AGAAACACTT CCTACCTCAA AACCCAGGTC 
GTCTCCCCTG ACGCCTTGTA CACTGCCAAG TTTGTCTCGT ACAATGACGA GGAGGATGAG 
GACACTACAG GCCTAGCAGG TCAGGTTAAG CTCTCCTCCA CCAGGAAGTC CTATGGCGAC 
CTCCCGCCCG CGCACAAGCC GCAGCCGCCT GCGCGCAAGC CCCGTCCTAT GTCTGATGGG 
ATCTTTCTGT CCAGCTCATT CCAGCCGCCG GCTGTCAATG CCAACAGTAC TGCAGGGCAG 
CCCCCTCCCC CTCCCATCAA ACCCAGCCTC ATCCCGACAG GCAACTTTAA AGGATTTAAT 
TCATACACTG GAAACACAAC AGGGGTTGTA GTATCAGGAG AAGTTTACAA GGACCTGCGA 
GAAAAGTGGA CGAAAGGTGG TCAAGAACAG GAAAACACTT TAACTGAAGG AGCCCCGGAG 
AGTATGACAT TCAAGGAGCG CCAGCGGCTC TTTTCCCAAG GGAAGGAAGT ATCCAACAAA 
GTCAAGGCCT CGCGAAAACT CATGGAGCTG GAAAATGAAC TCAATACAAA GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144421.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999896.1 Salmo salar afadin-like (LOC106571391), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAt47154
Clone ID Related Accession (Same CDS sequence)	XM_014144421.1
Accession Version	XM_014144421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGT TGCAGGACGA GGAGCGGCGG 
AGGAAGCAAC AGCTGGAGGA GATCCGTAAG CGCGAAGCGG AGGACCGGGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGG GCCAGACGAG AGGTCGATGA GAAGAGGAGA 
CAGGAGGAGG AATACTACAC ACGCCTGGAG GAAGGGCGGC GCCGGCAGCA CGAGGAGGCC 
GAGCGCAAGC TGCTGACGCC TGACGAGCCT GGTCTGTACA GACCTCCCCT CCCCAGGGAC 
TACCAGCCTC AGCCCCCCCC TCCCACTGCA TCCCCCGCAA CCACTGCCAC TAACGCTCCA 
CCACCCCCGC CTCAGAGAAA CACTTCCTAC CTCAAAACCC AGGTCGTCTC CCCTGACGCC 
TTGTACACTG CCAAGTTTGT CTCGTACAAT GACGAGGAGG ATGAGGACAC TACAGGCCTA 
GCAGGTCAGG TTAAGCTCTC CTCCACCAGG AAGTCCTATG GCGACCTCCC GCCCGCGCAC 
AAGCCGCAGC CGCCTGCGCG CAAGCCCCGT CCTATGTCTG ATGGGATCTT TCTGTCCAGC 
TCATTCCAGC CGCCGGCTGT CAATGCCAAC AGTACTGCAG GGCAGCCCCC TCCCCCTCCC 
ATCAAACCCA GCCTCATCCC GACAGGCAAC TTTAAAGGAT TTAATTCATA CACTGGAAAC 
ACAACAGGGG TTGTAGTATC AGGAGAAGTT TACAAGGACC TGCGAGAAAA GTGGACGAAA 
GGTGGTCAAG AACAGGAAAA CACTTTAACT GAAGGAGCCC CGGAGAGTAT GACATTCAAG 
GAGCGCCAGC GGCTCTTTTC CCAAGGGAAG GAAGTATCCA ACAAAGTCAA GGCCTCGCGA 
AAACTCATGG AGCTGGAAAA TGAACTCAAT ACAAAGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999896.1
CDS	233..6094
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQEIPEADQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGLYRPPLPRDYQPQPPPPTASPATTATNAPPPPPQRNTSYLKTQVVSPDALYTAKFVSYNDEEDEDTTGLAGQVKLSSTRKSYGDLPPAHKPQPPARKPRPMSDGIFLSSSFQPPAVNANSTAGQPPPPPIKPSLIPTGNFKGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144421.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144421.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGT TGCAGGACGA GGAGCGGCGG 
AGGAAGCAAC AGCTGGAGGA GATCCGTAAG CGCGAAGCGG AGGACCGGGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGG GCCAGACGAG AGGTCGATGA GAAGAGGAGA 
CAGGAGGAGG AATACTACAC ACGCCTGGAG GAAGGGCGGC GCCGGCAGCA CGAGGAGGCC 
GAGCGCAAGC TGCTGACGCC TGACGAGCCT GGTCTGTACA GACCTCCCCT CCCCAGGGAC 
TACCAGCCTC AGCCCCCCCC TCCCACTGCA TCCCCCGCAA CCACTGCCAC TAACGCTCCA 
CCACCCCCGC CTCAGAGAAA CACTTCCTAC CTCAAAACCC AGGTCGTCTC CCCTGACGCC 
TTGTACACTG CCAAGTTTGT CTCGTACAAT GACGAGGAGG ATGAGGACAC TACAGGCCTA 
GCAGGTCAGG TTAAGCTCTC CTCCACCAGG AAGTCCTATG GCGACCTCCC GCCCGCGCAC 
AAGCCGCAGC CGCCTGCGCG CAAGCCCCGT CCTATGTCTG ATGGGATCTT TCTGTCCAGC 
TCATTCCAGC CGCCGGCTGT CAATGCCAAC AGTACTGCAG GGCAGCCCCC TCCCCCTCCC 
ATCAAACCCA GCCTCATCCC GACAGGCAAC TTTAAAGGAT TTAATTCATA CACTGGAAAC 
ACAACAGGGG TTGTAGTATC AGGAGAAGTT TACAAGGACC TGCGAGAAAA GTGGACGAAA 
GGTGGTCAAG AACAGGAAAA CACTTTAACT GAAGGAGCCC CGGAGAGTAT GACATTCAAG 
GAGCGCCAGC GGCTCTTTTC CCAAGGGAAG GAAGTATCCA ACAAAGTCAA GGCCTCGCGA 
AAACTCATGG AGCTGGAAAA TGAACTCAAT ACAAAGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144423.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999898.1 Salmo salar afadin-like (LOC106571391), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OAt47155
Clone ID Related Accession (Same CDS sequence)	XM_014144423.1
Accession Version	XM_014144423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGACGGCTCA 
GACTCCAGGG ACAAACCGAA GCTCTACCGT CTGCAGCACA GTGTGACTGA GGTGGGCTCA 
GACACGACAG ATGACGGCTC CATCCAGCTG TTTGGTCCAG GTATCCTGCC CCACCACTGT 
AAACTGATGC ACACGGAGGG TTTGGTGACA GTGACACCAC ACAGTCTGGA GGCTGAGACC 
TACGTGGACG GCCAGCGTGT CGCCGAGACC ACCGTGCTGC GAAGCGGAGC CACACTGCAG 
TTCGGAGCCT CCCATGTCTT CAAGTTTGTG GACCCCAGCT ACGACCACAG CATGGGCAAG 
GGAGGGCCCG GACCAATGAT GAGAGGAAGG CACAAGTCAG GAAGTGTTCC TGAGGCGACC 
TTTGACCTGC AAGGGGACCT CCACAGTGGG ACTGCCCTCT CCACCAGCAA GAGCTCAGGG 
AAGCTGGAAA TGGAGCGTTC ATCCAACGGC AGCGAGAGGG GCATGGTCAA ACCCATGATC 
CGCGGGGAGC AGCAGGACAG CAGGTCCCAG GACAGCCTGA GCCCAGAAGT GACGCTGCCG 
GCCAGCATCG AGTTCAGGGA CAACTCTGAA GATACCTTCC TGACGGCCAT CATCAACTAC 
ACCAACAGCT CCACGGTGCA CTTCAAGCTG TCCCCCACCT ACGTACTGTA CATGACCTGT 
CGCTACGTCA TGTCCAGCCA CTACAGGCCC GACATCGGTC CCTCGGAGCG CACCCACAAG 
GTCATAGCCA TCGTCAACAA GATGGTGAGC ATGATGGAAG GAGTCATCCA GGAGATTCCT 
GAAGCGGACC AGAAGCAGAA GAACATTGCG GGGTCCCTGG CTTTCTGGAT GGCCAATGCC 
TCAGAGCTGC TCAACTTCAT CAAGCAGGAC AAGGACCTGT CGCGCATCAC ACTGGACGCC 
CAGGACGTGC TGGCACACCT CGTCCAGATG GCCTTCAAGT ACCTGGTCCA GTGTCTGCAG 
TCTGACCTGA GTAACTACAT GCCTGCCTTC CTGGACGACC CAGAGGAACA GAACCCTCAA 
AGGCCCAAAA TAGAGGATGT TTTGCACACC CTGACGGGCG CCATGTCGCT GCTGCGTCGT 
TGCCGTGTCA ACGCGGCGCT GACCATCCAG CTGTTCTCCC AGCTCTTTCA CTTCATCAAC 
ATGTGGCTGT TCAACAAGCT GGTGATGGAG GCTGACTCTG GACTGTGTTC ACACTACTGG 
GGCGCCATCC TCCGCCAGCA GCTCAGCCAC ATCGAGGCCT GGGCAGAGAA ACAGGGCCTG 
GAGCTGGCCG CCGACTGCCA CCTCAGTCGC ATCGTACAGG CTACGACTCT CCTCACCATG 
GACAAGTACT CCATGCAGGA CGTGCAGAAC ATCCACAACA CCTGCTTCAA GCTGAACTCC 
CTACAGCTCA ACGCCCTCAT GACCAACTAC CACTGTGCTC CCGACGAGCC TTACATCCCC 
CCAGAGCTCA TAGACCACGT GGTTGCCGTG GCGGAGAACA CAGCAGATGA GCTGGCCCGT 
AGCGACGGCC GAGAGGTCCA GCTAGAGGAG GACCCTGACC TGCAGCTGCC CTTCCTACTG 
CCCGAGGACG GCTACTCCTG CGACGTCGTG AGGAACATTC CCAACGGATT CCAGGAGTTC 
CTGGACCCAC TTTGTCAGAG AGGATTCTGT CGGCTAACCC CTCACCCGCG CTCCCCTGGG 
ACCTGGACCA TCCACTTTGA AGGAGTCGAC TGCGACAGCC ACTTCTCTGC AGACAACTCA 
GACCTGCAAA TGCGGAAGGA ACCGGAAGTT GTGACGGTCA CCCTGAAGAA GCACAATGGC 
ATGGGCCTCA GCATTGTGGC TGCCAAGGGT GCTGGGCAGG AGAAGCTCGG GATCTACATC 
AAGTCTGTTG TGAAGGGAGG CGCGGCTGAC GTGGACGGGC GTCTGGCTGC AGGGGACCAG 
CTGCTGAGTG TGGACGGCCG CAGCCTGGTG GGCCTCTCCC AGGAGAGGGC TGCGGAGTTG 
ATGACGAGGA CAGGTTCTGT GGTAACACTG GAGGTGGCTA AACAGGGAGC GATCTACCAC 
GGCCTGGCCA CGCTCCTCAA CCAGCCATCT CCTATGATGC CCAGAGCGTC GCAAGGAGGG 
CGTGACAAGA ATGGCAAACT GAGACCAAAG AGCGAAGGCT TTGAACTGTA CAACAACTCG 
GTCCAGAACG GCTCTCCAGA GAGCCCCCAG GCCGACTGGC AGGCCTACCC AGAACCAAAG 
AAGATGAGCG GGGAGGAGAG GCTCCTGAAG AACCGGGCCG ACCACCGCTC CAGCCCCAAC 
GTGGCCAACC AGAGCCAGAG TCCGGGAGGG AAGGCCGTCT ACACACGAGG ACCTGGGACC 
AAGATCACCT CTGTCTCCAC GGGCAACCTG TGTGTTGATG ACGAGCCCTC TCCTCCCCAT 
CCGGAGGCCT ATCCTATTCC CACTCAGACC TACCCCAGGG AATACTTCAC CATCCCAGCC 
TCCAAGTCTA CGAACCGGAT GGACCCGGCC CCAGGACAGT TATACGCTGA CGACAGGGGT 
CCTCCCGGCC CCATGGAAGA CCACCACCAT AACAGCATAG CTATGCAGCG GGTGGACCAC 
TCTCAGGAGG AGCTGTGTGA CCTCCCGGGG GGCTACCCTC CAACCAGCCT GCGTCCTGAG 
GACCTGGTCC ACTTGCACCA GCAGCAGCAG AGAGAGGCTG ACTACCAGTG GGGCAGTGGG 
GAACACTGGG TCAACCACCA CCAGGTCTCC TCTTCCGTGG AGTCCTCCAC CTCCAGCCAG 
GAGCACCTCA ACTACTCCTC GTCCTCCTCC AACAAGACCC AGAGCAACCA CAAGAGTGGC 
CCCGGACGCT GGAAGACCCC CGGCCCCATT GCCCCCCACC CAGGCCCCGT GGCCCAGTCC 
ACACGCACGG ACCTCCTTCC CCCTCCACCG CCCCCTCCCC CCGTACACTA CCAGGACTTT 
GAGCTCCAGC CCGACCTGCC CCTGCCCCCT CCTCCTTCGG TCACCCAGCA GCAGGCCGCA 
GCAGCAGCCG CCGACCGCAA GAAGCGCGAG GAGCAGCAGC GCTGGTATGA GAAGGAGAAG 
GCCCGGTTAG AGGAGGAGAG GGAGAGGAAG AGGAGGGAGC AGGAGAGGAA GCTGGGACAG 
GTCCGTAACC CAGCTGTGCC TGTAACCCAC AACAACAACC AACACGGGGG CACACTCCCC 
CCTCCCACAC AGCACCAGCA GGTGCCCCCT CCCCGGTCCC CCATGCAGCA GGTGCCCCCT 
CCCCGGTCCC CCATGCAGCA AGTGCCCCAT CCCTACCTCC CCCCCCAGGG CCAACAGCAG 
GCCCAGCAGC AGCAGCCCCC CAGGCCAGAG AAACTGTCCA CCCTGCCCAG AGCGGGCACC 
CTGCCCCCTC AGGGTAACCC CATGGACACA GTGATCAGAG AGCTGCTCCC CCAGCAGCAG 
CCCCGCACCA TCGAGCGCCG GGACCTGCAG TACATCACCA TCAGCAAGGA GGAGCTGTCC 
TCCAGCGACA GCCTGTCCCC AGACCCCTGG AAGAGGGATG CCCGGGAGAA GTTGGAGAAG 
CAGCAGCAGC TGCACATCGT AGACCTGCTG GACAGGGAGA TCCAGGACCT GCAGGCCAAG 
CCGGAGCGTA CGGCCGAGGA GAGCGACCGG CTCAGGAAGT TGATGTTGGA GTGGCAGTTC 
CAGAAGAGAC TGCAGGAGTC CAAGCACAGT GATGAGGAAG ATGAGGAGGA TGACGACGAG 
GACGTGGACA CCATGATGAT CATGCAGCGG CTGGAGGCAG AGAAGAGAGC AAGGCAGACT 
GCTGTCCCAG CAATCTCTGT TCTAGACTTG TTGCAGGACG AGGAGCGGCG GAGGAAGCAA 
CAGCTGGAGG AGATCCGTAA GCGCGAAGCG GAGGACCGGG CCAAACAGGA GGAGGAGAGA 
CGCTGGAGAG AGGAGGAGAG GGCCAGACGA GAGGTCGATG AGAAGAGGAG ACAGGAGGAG 
GAATACTACA CACGCCTGGA GGAAGGGCGG CGCCGGCAGC ACGAGGAGGC CGAGCGCAAG 
CTGCTGACGC CTGACGAGCC TGGTCTGTAC AGACCTCCCC TCCCCAGGGA CTACCAGCCT 
CAGCCCCCCC CTCCCACTGC ATCCCCCGCA ACCACTGCCA CTAACGCTCC ACCACCCCCG 
CCTCAGAGAA ACACTTCCTA CCTCAAAACC CAGGTCGTCT CCCCTGACGC CTTGTACACT 
GCCAAGTTTG TCTCGTACAA TGACGAGGAG GATGAGGACA CTACAGGCCT AGCAGGTCAG 
GTTAAGCTCT CCTCCACCAG GAAGTCCTAT GGCGACCTCC CGCCCGCGCA CAAGCCGCAG 
CCGCCTGCGC GCAAGCCCCG TCCTATGTCT GATGGGATCT TTCTGTCCAG CTCATTCCAG 
CCGCCGGCTG TCAATGCCAA CAGTACTGCA GGGCAGCCCC CTCCCCCTCC CATCAAACCC 
AGCCTCATCC CGACAGGCAA CTTTAAAGGA TTTAATTCAT ACACTGGAAA CACAACAGGG 
GTTGTAGTAT CAGGAGAAGT TTACAAGGAC CTGCGAGAAA AGTGGACGAA AGGTGGTCAA 
GAACAGGAAA ACACTTTAAC TGAAGGAGCC CCGGAGAGTA TGACATTCAA GGAGCGCCAG 
CGGCTCTTTT CCCAAGGGAA GGAAGTATCC AACAAAGTCA AGGCCTCGCG AAAACTCATG 
GAGCTGGAAA ATGAACTCAA TACAAAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999898.1
CDS	233..6085
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQEIPEADQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGLYRPPLPRDYQPQPPPPTASPATTATNAPPPPPQRNTSYLKTQVVSPDALYTAKFVSYNDEEDEDTTGLAGQVKLSSTRKSYGDLPPAHKPQPPARKPRPMSDGIFLSSSFQPPAVNANSTAGQPPPPPIKPSLIPTGNFKGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144423.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144423.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGACGGCTCA 
GACTCCAGGG ACAAACCGAA GCTCTACCGT CTGCAGCACA GTGTGACTGA GGTGGGCTCA 
GACACGACAG ATGACGGCTC CATCCAGCTG TTTGGTCCAG GTATCCTGCC CCACCACTGT 
AAACTGATGC ACACGGAGGG TTTGGTGACA GTGACACCAC ACAGTCTGGA GGCTGAGACC 
TACGTGGACG GCCAGCGTGT CGCCGAGACC ACCGTGCTGC GAAGCGGAGC CACACTGCAG 
TTCGGAGCCT CCCATGTCTT CAAGTTTGTG GACCCCAGCT ACGACCACAG CATGGGCAAG 
GGAGGGCCCG GACCAATGAT GAGAGGAAGG CACAAGTCAG GAAGTGTTCC TGAGGCGACC 
TTTGACCTGC AAGGGGACCT CCACAGTGGG ACTGCCCTCT CCACCAGCAA GAGCTCAGGG 
AAGCTGGAAA TGGAGCGTTC ATCCAACGGC AGCGAGAGGG GCATGGTCAA ACCCATGATC 
CGCGGGGAGC AGCAGGACAG CAGGTCCCAG GACAGCCTGA GCCCAGAAGT GACGCTGCCG 
GCCAGCATCG AGTTCAGGGA CAACTCTGAA GATACCTTCC TGACGGCCAT CATCAACTAC 
ACCAACAGCT CCACGGTGCA CTTCAAGCTG TCCCCCACCT ACGTACTGTA CATGACCTGT 
CGCTACGTCA TGTCCAGCCA CTACAGGCCC GACATCGGTC CCTCGGAGCG CACCCACAAG 
GTCATAGCCA TCGTCAACAA GATGGTGAGC ATGATGGAAG GAGTCATCCA GGAGATTCCT 
GAAGCGGACC AGAAGCAGAA GAACATTGCG GGGTCCCTGG CTTTCTGGAT GGCCAATGCC 
TCAGAGCTGC TCAACTTCAT CAAGCAGGAC AAGGACCTGT CGCGCATCAC ACTGGACGCC 
CAGGACGTGC TGGCACACCT CGTCCAGATG GCCTTCAAGT ACCTGGTCCA GTGTCTGCAG 
TCTGACCTGA GTAACTACAT GCCTGCCTTC CTGGACGACC CAGAGGAACA GAACCCTCAA 
AGGCCCAAAA TAGAGGATGT TTTGCACACC CTGACGGGCG CCATGTCGCT GCTGCGTCGT 
TGCCGTGTCA ACGCGGCGCT GACCATCCAG CTGTTCTCCC AGCTCTTTCA CTTCATCAAC 
ATGTGGCTGT TCAACAAGCT GGTGATGGAG GCTGACTCTG GACTGTGTTC ACACTACTGG 
GGCGCCATCC TCCGCCAGCA GCTCAGCCAC ATCGAGGCCT GGGCAGAGAA ACAGGGCCTG 
GAGCTGGCCG CCGACTGCCA CCTCAGTCGC ATCGTACAGG CTACGACTCT CCTCACCATG 
GACAAGTACT CCATGCAGGA CGTGCAGAAC ATCCACAACA CCTGCTTCAA GCTGAACTCC 
CTACAGCTCA ACGCCCTCAT GACCAACTAC CACTGTGCTC CCGACGAGCC TTACATCCCC 
CCAGAGCTCA TAGACCACGT GGTTGCCGTG GCGGAGAACA CAGCAGATGA GCTGGCCCGT 
AGCGACGGCC GAGAGGTCCA GCTAGAGGAG GACCCTGACC TGCAGCTGCC CTTCCTACTG 
CCCGAGGACG GCTACTCCTG CGACGTCGTG AGGAACATTC CCAACGGATT CCAGGAGTTC 
CTGGACCCAC TTTGTCAGAG AGGATTCTGT CGGCTAACCC CTCACCCGCG CTCCCCTGGG 
ACCTGGACCA TCCACTTTGA AGGAGTCGAC TGCGACAGCC ACTTCTCTGC AGACAACTCA 
GACCTGCAAA TGCGGAAGGA ACCGGAAGTT GTGACGGTCA CCCTGAAGAA GCACAATGGC 
ATGGGCCTCA GCATTGTGGC TGCCAAGGGT GCTGGGCAGG AGAAGCTCGG GATCTACATC 
AAGTCTGTTG TGAAGGGAGG CGCGGCTGAC GTGGACGGGC GTCTGGCTGC AGGGGACCAG 
CTGCTGAGTG TGGACGGCCG CAGCCTGGTG GGCCTCTCCC AGGAGAGGGC TGCGGAGTTG 
ATGACGAGGA CAGGTTCTGT GGTAACACTG GAGGTGGCTA AACAGGGAGC GATCTACCAC 
GGCCTGGCCA CGCTCCTCAA CCAGCCATCT CCTATGATGC CCAGAGCGTC GCAAGGAGGG 
CGTGACAAGA ATGGCAAACT GAGACCAAAG AGCGAAGGCT TTGAACTGTA CAACAACTCG 
GTCCAGAACG GCTCTCCAGA GAGCCCCCAG GCCGACTGGC AGGCCTACCC AGAACCAAAG 
AAGATGAGCG GGGAGGAGAG GCTCCTGAAG AACCGGGCCG ACCACCGCTC CAGCCCCAAC 
GTGGCCAACC AGAGCCAGAG TCCGGGAGGG AAGGCCGTCT ACACACGAGG ACCTGGGACC 
AAGATCACCT CTGTCTCCAC GGGCAACCTG TGTGTTGATG ACGAGCCCTC TCCTCCCCAT 
CCGGAGGCCT ATCCTATTCC CACTCAGACC TACCCCAGGG AATACTTCAC CATCCCAGCC 
TCCAAGTCTA CGAACCGGAT GGACCCGGCC CCAGGACAGT TATACGCTGA CGACAGGGGT 
CCTCCCGGCC CCATGGAAGA CCACCACCAT AACAGCATAG CTATGCAGCG GGTGGACCAC 
TCTCAGGAGG AGCTGTGTGA CCTCCCGGGG GGCTACCCTC CAACCAGCCT GCGTCCTGAG 
GACCTGGTCC ACTTGCACCA GCAGCAGCAG AGAGAGGCTG ACTACCAGTG GGGCAGTGGG 
GAACACTGGG TCAACCACCA CCAGGTCTCC TCTTCCGTGG AGTCCTCCAC CTCCAGCCAG 
GAGCACCTCA ACTACTCCTC GTCCTCCTCC AACAAGACCC AGAGCAACCA CAAGAGTGGC 
CCCGGACGCT GGAAGACCCC CGGCCCCATT GCCCCCCACC CAGGCCCCGT GGCCCAGTCC 
ACACGCACGG ACCTCCTTCC CCCTCCACCG CCCCCTCCCC CCGTACACTA CCAGGACTTT 
GAGCTCCAGC CCGACCTGCC CCTGCCCCCT CCTCCTTCGG TCACCCAGCA GCAGGCCGCA 
GCAGCAGCCG CCGACCGCAA GAAGCGCGAG GAGCAGCAGC GCTGGTATGA GAAGGAGAAG 
GCCCGGTTAG AGGAGGAGAG GGAGAGGAAG AGGAGGGAGC AGGAGAGGAA GCTGGGACAG 
GTCCGTAACC CAGCTGTGCC TGTAACCCAC AACAACAACC AACACGGGGG CACACTCCCC 
CCTCCCACAC AGCACCAGCA GGTGCCCCCT CCCCGGTCCC CCATGCAGCA GGTGCCCCCT 
CCCCGGTCCC CCATGCAGCA AGTGCCCCAT CCCTACCTCC CCCCCCAGGG CCAACAGCAG 
GCCCAGCAGC AGCAGCCCCC CAGGCCAGAG AAACTGTCCA CCCTGCCCAG AGCGGGCACC 
CTGCCCCCTC AGGGTAACCC CATGGACACA GTGATCAGAG AGCTGCTCCC CCAGCAGCAG 
CCCCGCACCA TCGAGCGCCG GGACCTGCAG TACATCACCA TCAGCAAGGA GGAGCTGTCC 
TCCAGCGACA GCCTGTCCCC AGACCCCTGG AAGAGGGATG CCCGGGAGAA GTTGGAGAAG 
CAGCAGCAGC TGCACATCGT AGACCTGCTG GACAGGGAGA TCCAGGACCT GCAGGCCAAG 
CCGGAGCGTA CGGCCGAGGA GAGCGACCGG CTCAGGAAGT TGATGTTGGA GTGGCAGTTC 
CAGAAGAGAC TGCAGGAGTC CAAGCACAGT GATGAGGAAG ATGAGGAGGA TGACGACGAG 
GACGTGGACA CCATGATGAT CATGCAGCGG CTGGAGGCAG AGAAGAGAGC AAGGCAGACT 
GCTGTCCCAG CAATCTCTGT TCTAGACTTG TTGCAGGACG AGGAGCGGCG GAGGAAGCAA 
CAGCTGGAGG AGATCCGTAA GCGCGAAGCG GAGGACCGGG CCAAACAGGA GGAGGAGAGA 
CGCTGGAGAG AGGAGGAGAG GGCCAGACGA GAGGTCGATG AGAAGAGGAG ACAGGAGGAG 
GAATACTACA CACGCCTGGA GGAAGGGCGG CGCCGGCAGC ACGAGGAGGC CGAGCGCAAG 
CTGCTGACGC CTGACGAGCC TGGTCTGTAC AGACCTCCCC TCCCCAGGGA CTACCAGCCT 
CAGCCCCCCC CTCCCACTGC ATCCCCCGCA ACCACTGCCA CTAACGCTCC ACCACCCCCG 
CCTCAGAGAA ACACTTCCTA CCTCAAAACC CAGGTCGTCT CCCCTGACGC CTTGTACACT 
GCCAAGTTTG TCTCGTACAA TGACGAGGAG GATGAGGACA CTACAGGCCT AGCAGGTCAG 
GTTAAGCTCT CCTCCACCAG GAAGTCCTAT GGCGACCTCC CGCCCGCGCA CAAGCCGCAG 
CCGCCTGCGC GCAAGCCCCG TCCTATGTCT GATGGGATCT TTCTGTCCAG CTCATTCCAG 
CCGCCGGCTG TCAATGCCAA CAGTACTGCA GGGCAGCCCC CTCCCCCTCC CATCAAACCC 
AGCCTCATCC CGACAGGCAA CTTTAAAGGA TTTAATTCAT ACACTGGAAA CACAACAGGG 
GTTGTAGTAT CAGGAGAAGT TTACAAGGAC CTGCGAGAAA AGTGGACGAA AGGTGGTCAA 
GAACAGGAAA ACACTTTAAC TGAAGGAGCC CCGGAGAGTA TGACATTCAA GGAGCGCCAG 
CGGCTCTTTT CCCAAGGGAA GGAAGTATCC AACAAAGTCA AGGCCTCGCG AAAACTCATG 
GAGCTGGAAA ATGAACTCAA TACAAAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144424.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999899.1 Salmo salar afadin-like (LOC106571391), transcript variant X7, mRNA.

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CloneID	OAt47156
Clone ID Related Accession (Same CDS sequence)	XM_014144424.1
Accession Version	XM_014144424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGACGGCTCA 
GACTCCAGGG ACAAACCGAA GCTCTACCGT CTGCAGCACA GTGTGACTGA GGTGGGCTCA 
GACACGACAG ATGACGGCTC CATCCAGCTG TTTGGTCCAG GTATCCTGCC CCACCACTGT 
AAACTGATGC ACACGGAGGG TTTGGTGACA GTGACACCAC ACAGTCTGGA GGCTGAGACC 
TACGTGGACG GCCAGCGTGT CGCCGAGACC ACCGTGCTGC GAAGCGGAGC CACACTGCAG 
TTCGGAGCCT CCCATGTCTT CAAGTTTGTG GACCCCAGCT ACGACCACAG CATGGGCAAG 
GGAGGGCCCG GACCAATGAT GAGAGGAAGG CACAAGTCAG GAAGTGTTCC TGAGGCGACC 
TTTGACCTGC AAGGGGACCT CCACAGTGGG ACTGCCCTCT CCACCAGCAA GAGCTCAGGG 
AAGCTGGAAA TGGAGCGTTC ATCCAACGGC AGCGAGAGGG GCATGGTCAA ACCCATGATC 
CGCGGGGAGC AGCAGGACAG CAGGTCCCAG GACAGCCTGA GCCCAGAAGT GACGCTGCCG 
GCCAGCATCG AGTTCAGGGA CAACTCTGAA GATACCTTCC TGACGGCCAT CATCAACTAC 
ACCAACAGCT CCACGGTGCA CTTCAAGCTG TCCCCCACCT ACGTACTGTA CATGACCTGT 
CGCTACGTCA TGTCCAGCCA CTACAGGCCC GACATCGGTC CCTCGGAGCG CACCCACAAG 
GTCATAGCCA TCGTCAACAA GATGGTGAGC ATGATGGAAG GAGTCATCCA GAAGCAGAAG 
AACATTGCGG GGTCCCTGGC TTTCTGGATG GCCAATGCCT CAGAGCTGCT CAACTTCATC 
AAGCAGGACA AGGACCTGTC GCGCATCACA CTGGACGCCC AGGACGTGCT GGCACACCTC 
GTCCAGATGG CCTTCAAGTA CCTGGTCCAG TGTCTGCAGT CTGACCTGAG TAACTACATG 
CCTGCCTTCC TGGACGACCC AGAGGAACAG AACCCTCAAA GGCCCAAAAT AGAGGATGTT 
TTGCACACCC TGACGGGCGC CATGTCGCTG CTGCGTCGTT GCCGTGTCAA CGCGGCGCTG 
ACCATCCAGC TGTTCTCCCA GCTCTTTCAC TTCATCAACA TGTGGCTGTT CAACAAGCTG 
GTGATGGAGG CTGACTCTGG ACTGTGTTCA CACTACTGGG GCGCCATCCT CCGCCAGCAG 
CTCAGCCACA TCGAGGCCTG GGCAGAGAAA CAGGGCCTGG AGCTGGCCGC CGACTGCCAC 
CTCAGTCGCA TCGTACAGGC TACGACTCTC CTCACCATGG ACAAGTACTC CATGCAGGAC 
GTGCAGAACA TCCACAACAC CTGCTTCAAG CTGAACTCCC TACAGCTCAA CGCCCTCATG 
ACCAACTACC ACTGTGCTCC CGACGAGCCT TACATCCCCC CAGAGCTCAT AGACCACGTG 
GTTGCCGTGG CGGAGAACAC AGCAGATGAG CTGGCCCGTA GCGACGGCCG AGAGGTCCAG 
CTAGAGGAGG ACCCTGACCT GCAGCTGCCC TTCCTACTGC CCGAGGACGG CTACTCCTGC 
GACGTCGTGA GGAACATTCC CAACGGATTC CAGGAGTTCC TGGACCCACT TTGTCAGAGA 
GGATTCTGTC GGCTAACCCC TCACCCGCGC TCCCCTGGGA CCTGGACCAT CCACTTTGAA 
GGAGTCGACT GCGACAGCCA CTTCTCTGCA GACAACTCAG ACCTGCAAAT GCGGAAGGAA 
CCGGAAGTTG TGACGGTCAC CCTGAAGAAG CACAATGGCA TGGGCCTCAG CATTGTGGCT 
GCCAAGGGTG CTGGGCAGGA GAAGCTCGGG ATCTACATCA AGTCTGTTGT GAAGGGAGGC 
GCGGCTGACG TGGACGGGCG TCTGGCTGCA GGGGACCAGC TGCTGAGTGT GGACGGCCGC 
AGCCTGGTGG GCCTCTCCCA GGAGAGGGCT GCGGAGTTGA TGACGAGGAC AGGTTCTGTG 
GTAACACTGG AGGTGGCTAA ACAGGGAGCG ATCTACCACG GCCTGGCCAC GCTCCTCAAC 
CAGCCATCTC CTATGATGCC CAGAGCGTCG CAAGGAGGGC GTGACAAGAA TGGCAAACTG 
AGACCAAAGA GCGAAGGCTT TGAACTGTAC AACAACTCGG TCCAGAACGG CTCTCCAGAG 
AGCCCCCAGG CCGACTGGCA GGCCTACCCA GAACCAAAGA AGATGAGCGG GGAGGAGAGG 
CTCCTGAAGA ACCGGGCCGA CCACCGCTCC AGCCCCAACG TGGCCAACCA GAGCCAGAGT 
CCGGGAGGGA AGGCCGTCTA CACACGAGGA CCTGGGACCA AGATCACCTC TGTCTCCACG 
GGCAACCTGT GTGTTGATGA CGAGCCCTCT CCTCCCCATC CGGAGGCCTA TCCTATTCCC 
ACTCAGACCT ACCCCAGGGA ATACTTCACC ATCCCAGCCT CCAAGTCTAC GAACCGGATG 
GACCCGGCCC CAGGACAGTT ATACGCTGAC GACAGGGGTC CTCCCGGCCC CATGGAAGAC 
CACCACCATA ACAGCATAGC TATGCAGCGG GTGGACCACT CTCAGGAGGA GCTGTGTGAC 
CTCCCGGGGG GCTACCCTCC AACCAGCCTG CGTCCTGAGG ACCTGGTCCA CTTGCACCAG 
CAGCAGCAGA GAGAGGCTGA CTACCAGTGG GGCAGTGGGG AACACTGGGT CAACCACCAC 
CAGGTCTCCT CTTCCGTGGA GTCCTCCACC TCCAGCCAGG AGCACCTCAA CTACTCCTCG 
TCCTCCTCCA ACAAGACCCA GAGCAACCAC AAGAGTGGCC CCGGACGCTG GAAGACCCCC 
GGCCCCATTG CCCCCCACCC AGGCCCCGTG GCCCAGTCCA CACGCACGGA CCTCCTTCCC 
CCTCCACCGC CCCCTCCCCC CGTACACTAC CAGGACTTTG AGCTCCAGCC CGACCTGCCC 
CTGCCCCCTC CTCCTTCGGT CACCCAGCAG CAGGCCGCAG CAGCAGCCGC CGACCGCAAG 
AAGCGCGAGG AGCAGCAGCG CTGGTATGAG AAGGAGAAGG CCCGGTTAGA GGAGGAGAGG 
GAGAGGAAGA GGAGGGAGCA GGAGAGGAAG CTGGGACAGG TCCGTAACCC AGCTGTGCCT 
GTAACCCACA ACAACAACCA ACACGGGGGC ACACTCCCCC CTCCCACACA GCACCAGCAG 
GTGCCCCCTC CCCGGTCCCC CATGCAGCAG GTGCCCCCTC CCCGGTCCCC CATGCAGCAA 
GTGCCCCATC CCTACCTCCC CCCCCAGGGC CAACAGCAGG CCCAGCAGCA GCAGCCCCCC 
AGGCCAGAGA AACTGTCCAC CCTGCCCAGA GCGGGCACCC TGCCCCCTCA GGGTAACCCC 
ATGGACACAG TGATCAGAGA GCTGCTCCCC CAGCAGCAGC CCCGCACCAT CGAGCGCCGG 
GACCTGCAGT ACATCACCAT CAGCAAGGAG GAGCTGTCCT CCAGCGACAG CCTGTCCCCA 
GACCCCTGGA AGAGGGATGC CCGGGAGAAG TTGGAGAAGC AGCAGCAGCT GCACATCGTA 
GACCTGCTGG ACAGGGAGAT CCAGGACCTG CAGGCCAAGC CGGAGCGTAC GGCCGAGGAG 
AGCGACCGGC TCAGGAAGTT GATGTTGGAG TGGCAGTTCC AGAAGAGACT GCAGGAGTCC 
AAGCACAGTG ATGAGGAAGA TGAGGAGGAT GACGACGAGG ACGTGGACAC CATGATGATC 
ATGCAGCGGC TGGAGGCAGA GAAGAGAGCA AGGCAGACTG CTGTCCCAGC AATCTCTGTT 
CTAGACTTGT TGCAGGACGA GGAGCGGCGG AGGAAGCAAC AGCTGGAGGA GATCCGTAAG 
CGCGAAGCGG AGGACCGGGC CAAACAGGAG GAGGAGAGAC GCTGGAGAGA GGAGGAGAGG 
GCCAGACGAG AGGTCGATGA GAAGAGGAGA CAGGAGGAGG AATACTACAC ACGCCTGGAG 
GAAGGGCGGC GCCGGCAGCA CGAGGAGGCC GAGCGCAAGC TGCTGACGCC TGACGAGCCT 
GGTCTGTACA GACCTCCCCT CCCCAGGGAC TACCAGCCTC AGCCCCCCCC TCCCACTGCA 
TCCCCCGCAA CCACTGCCAC TAACGCTCCA CCACCCCCGC CTCAGAGAAA CACTTCCTAC 
CTCAAAACCC AGGTCGTCTC CCCTGACGCC TTGTACACTG CCAAGTTTGT CTCGTACAAT 
GACGAGGAGG ATGAGGACAC TACAGGCCTA GCAGGTCAGG TTAAGCTCTC CTCCACCAGG 
AAGTCCTATG GCGACCTCCC GCCCGCGCAC AAGCCGCAGC CGCCTGCGCG CAAGCCCCGT 
CCTATGTCTG ATGGGATCTT TCTGTCCAGC TCATTCCAGC CGCCGGCTGT CAATGCCAAC 
AGTACTGCAG GGCAGCCCCC TCCCCCTCCC ATCAAACCCA GCCTCATCCC GACAGGCAAC 
TTTAAAGGAT TTAATTCATA CACTGGAAAC ACAACAGGGG TTGTAGTATC AGGAGAAGTT 
TACAAGGACC TGCGAGAAAA GTGGACGAAA GGTGGTCAAG AACAGGAAAA CACTTTAACT 
GAAGGAGCCC CGGAGAGTAT GACATTCAAG GAGCGCCAGC GGCTCTTTTC CCAAGGGAAG 
GAAGTATCCA ACAAAGTCAA GGCCTCGCGA AAACTCATGG AGCTGGAAAA TGAACTCAAT 
ACAAAGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999899.1
CDS	233..6064
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGLYRPPLPRDYQPQPPPPTASPATTATNAPPPPPQRNTSYLKTQVVSPDALYTAKFVSYNDEEDEDTTGLAGQVKLSSTRKSYGDLPPAHKPQPPARKPRPMSDGIFLSSSFQPPAVNANSTAGQPPPPPIKPSLIPTGNFKGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144424.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144424.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGACGGCTCA 
GACTCCAGGG ACAAACCGAA GCTCTACCGT CTGCAGCACA GTGTGACTGA GGTGGGCTCA 
GACACGACAG ATGACGGCTC CATCCAGCTG TTTGGTCCAG GTATCCTGCC CCACCACTGT 
AAACTGATGC ACACGGAGGG TTTGGTGACA GTGACACCAC ACAGTCTGGA GGCTGAGACC 
TACGTGGACG GCCAGCGTGT CGCCGAGACC ACCGTGCTGC GAAGCGGAGC CACACTGCAG 
TTCGGAGCCT CCCATGTCTT CAAGTTTGTG GACCCCAGCT ACGACCACAG CATGGGCAAG 
GGAGGGCCCG GACCAATGAT GAGAGGAAGG CACAAGTCAG GAAGTGTTCC TGAGGCGACC 
TTTGACCTGC AAGGGGACCT CCACAGTGGG ACTGCCCTCT CCACCAGCAA GAGCTCAGGG 
AAGCTGGAAA TGGAGCGTTC ATCCAACGGC AGCGAGAGGG GCATGGTCAA ACCCATGATC 
CGCGGGGAGC AGCAGGACAG CAGGTCCCAG GACAGCCTGA GCCCAGAAGT GACGCTGCCG 
GCCAGCATCG AGTTCAGGGA CAACTCTGAA GATACCTTCC TGACGGCCAT CATCAACTAC 
ACCAACAGCT CCACGGTGCA CTTCAAGCTG TCCCCCACCT ACGTACTGTA CATGACCTGT 
CGCTACGTCA TGTCCAGCCA CTACAGGCCC GACATCGGTC CCTCGGAGCG CACCCACAAG 
GTCATAGCCA TCGTCAACAA GATGGTGAGC ATGATGGAAG GAGTCATCCA GAAGCAGAAG 
AACATTGCGG GGTCCCTGGC TTTCTGGATG GCCAATGCCT CAGAGCTGCT CAACTTCATC 
AAGCAGGACA AGGACCTGTC GCGCATCACA CTGGACGCCC AGGACGTGCT GGCACACCTC 
GTCCAGATGG CCTTCAAGTA CCTGGTCCAG TGTCTGCAGT CTGACCTGAG TAACTACATG 
CCTGCCTTCC TGGACGACCC AGAGGAACAG AACCCTCAAA GGCCCAAAAT AGAGGATGTT 
TTGCACACCC TGACGGGCGC CATGTCGCTG CTGCGTCGTT GCCGTGTCAA CGCGGCGCTG 
ACCATCCAGC TGTTCTCCCA GCTCTTTCAC TTCATCAACA TGTGGCTGTT CAACAAGCTG 
GTGATGGAGG CTGACTCTGG ACTGTGTTCA CACTACTGGG GCGCCATCCT CCGCCAGCAG 
CTCAGCCACA TCGAGGCCTG GGCAGAGAAA CAGGGCCTGG AGCTGGCCGC CGACTGCCAC 
CTCAGTCGCA TCGTACAGGC TACGACTCTC CTCACCATGG ACAAGTACTC CATGCAGGAC 
GTGCAGAACA TCCACAACAC CTGCTTCAAG CTGAACTCCC TACAGCTCAA CGCCCTCATG 
ACCAACTACC ACTGTGCTCC CGACGAGCCT TACATCCCCC CAGAGCTCAT AGACCACGTG 
GTTGCCGTGG CGGAGAACAC AGCAGATGAG CTGGCCCGTA GCGACGGCCG AGAGGTCCAG 
CTAGAGGAGG ACCCTGACCT GCAGCTGCCC TTCCTACTGC CCGAGGACGG CTACTCCTGC 
GACGTCGTGA GGAACATTCC CAACGGATTC CAGGAGTTCC TGGACCCACT TTGTCAGAGA 
GGATTCTGTC GGCTAACCCC TCACCCGCGC TCCCCTGGGA CCTGGACCAT CCACTTTGAA 
GGAGTCGACT GCGACAGCCA CTTCTCTGCA GACAACTCAG ACCTGCAAAT GCGGAAGGAA 
CCGGAAGTTG TGACGGTCAC CCTGAAGAAG CACAATGGCA TGGGCCTCAG CATTGTGGCT 
GCCAAGGGTG CTGGGCAGGA GAAGCTCGGG ATCTACATCA AGTCTGTTGT GAAGGGAGGC 
GCGGCTGACG TGGACGGGCG TCTGGCTGCA GGGGACCAGC TGCTGAGTGT GGACGGCCGC 
AGCCTGGTGG GCCTCTCCCA GGAGAGGGCT GCGGAGTTGA TGACGAGGAC AGGTTCTGTG 
GTAACACTGG AGGTGGCTAA ACAGGGAGCG ATCTACCACG GCCTGGCCAC GCTCCTCAAC 
CAGCCATCTC CTATGATGCC CAGAGCGTCG CAAGGAGGGC GTGACAAGAA TGGCAAACTG 
AGACCAAAGA GCGAAGGCTT TGAACTGTAC AACAACTCGG TCCAGAACGG CTCTCCAGAG 
AGCCCCCAGG CCGACTGGCA GGCCTACCCA GAACCAAAGA AGATGAGCGG GGAGGAGAGG 
CTCCTGAAGA ACCGGGCCGA CCACCGCTCC AGCCCCAACG TGGCCAACCA GAGCCAGAGT 
CCGGGAGGGA AGGCCGTCTA CACACGAGGA CCTGGGACCA AGATCACCTC TGTCTCCACG 
GGCAACCTGT GTGTTGATGA CGAGCCCTCT CCTCCCCATC CGGAGGCCTA TCCTATTCCC 
ACTCAGACCT ACCCCAGGGA ATACTTCACC ATCCCAGCCT CCAAGTCTAC GAACCGGATG 
GACCCGGCCC CAGGACAGTT ATACGCTGAC GACAGGGGTC CTCCCGGCCC CATGGAAGAC 
CACCACCATA ACAGCATAGC TATGCAGCGG GTGGACCACT CTCAGGAGGA GCTGTGTGAC 
CTCCCGGGGG GCTACCCTCC AACCAGCCTG CGTCCTGAGG ACCTGGTCCA CTTGCACCAG 
CAGCAGCAGA GAGAGGCTGA CTACCAGTGG GGCAGTGGGG AACACTGGGT CAACCACCAC 
CAGGTCTCCT CTTCCGTGGA GTCCTCCACC TCCAGCCAGG AGCACCTCAA CTACTCCTCG 
TCCTCCTCCA ACAAGACCCA GAGCAACCAC AAGAGTGGCC CCGGACGCTG GAAGACCCCC 
GGCCCCATTG CCCCCCACCC AGGCCCCGTG GCCCAGTCCA CACGCACGGA CCTCCTTCCC 
CCTCCACCGC CCCCTCCCCC CGTACACTAC CAGGACTTTG AGCTCCAGCC CGACCTGCCC 
CTGCCCCCTC CTCCTTCGGT CACCCAGCAG CAGGCCGCAG CAGCAGCCGC CGACCGCAAG 
AAGCGCGAGG AGCAGCAGCG CTGGTATGAG AAGGAGAAGG CCCGGTTAGA GGAGGAGAGG 
GAGAGGAAGA GGAGGGAGCA GGAGAGGAAG CTGGGACAGG TCCGTAACCC AGCTGTGCCT 
GTAACCCACA ACAACAACCA ACACGGGGGC ACACTCCCCC CTCCCACACA GCACCAGCAG 
GTGCCCCCTC CCCGGTCCCC CATGCAGCAG GTGCCCCCTC CCCGGTCCCC CATGCAGCAA 
GTGCCCCATC CCTACCTCCC CCCCCAGGGC CAACAGCAGG CCCAGCAGCA GCAGCCCCCC 
AGGCCAGAGA AACTGTCCAC CCTGCCCAGA GCGGGCACCC TGCCCCCTCA GGGTAACCCC 
ATGGACACAG TGATCAGAGA GCTGCTCCCC CAGCAGCAGC CCCGCACCAT CGAGCGCCGG 
GACCTGCAGT ACATCACCAT CAGCAAGGAG GAGCTGTCCT CCAGCGACAG CCTGTCCCCA 
GACCCCTGGA AGAGGGATGC CCGGGAGAAG TTGGAGAAGC AGCAGCAGCT GCACATCGTA 
GACCTGCTGG ACAGGGAGAT CCAGGACCTG CAGGCCAAGC CGGAGCGTAC GGCCGAGGAG 
AGCGACCGGC TCAGGAAGTT GATGTTGGAG TGGCAGTTCC AGAAGAGACT GCAGGAGTCC 
AAGCACAGTG ATGAGGAAGA TGAGGAGGAT GACGACGAGG ACGTGGACAC CATGATGATC 
ATGCAGCGGC TGGAGGCAGA GAAGAGAGCA AGGCAGACTG CTGTCCCAGC AATCTCTGTT 
CTAGACTTGT TGCAGGACGA GGAGCGGCGG AGGAAGCAAC AGCTGGAGGA GATCCGTAAG 
CGCGAAGCGG AGGACCGGGC CAAACAGGAG GAGGAGAGAC GCTGGAGAGA GGAGGAGAGG 
GCCAGACGAG AGGTCGATGA GAAGAGGAGA CAGGAGGAGG AATACTACAC ACGCCTGGAG 
GAAGGGCGGC GCCGGCAGCA CGAGGAGGCC GAGCGCAAGC TGCTGACGCC TGACGAGCCT 
GGTCTGTACA GACCTCCCCT CCCCAGGGAC TACCAGCCTC AGCCCCCCCC TCCCACTGCA 
TCCCCCGCAA CCACTGCCAC TAACGCTCCA CCACCCCCGC CTCAGAGAAA CACTTCCTAC 
CTCAAAACCC AGGTCGTCTC CCCTGACGCC TTGTACACTG CCAAGTTTGT CTCGTACAAT 
GACGAGGAGG ATGAGGACAC TACAGGCCTA GCAGGTCAGG TTAAGCTCTC CTCCACCAGG 
AAGTCCTATG GCGACCTCCC GCCCGCGCAC AAGCCGCAGC CGCCTGCGCG CAAGCCCCGT 
CCTATGTCTG ATGGGATCTT TCTGTCCAGC TCATTCCAGC CGCCGGCTGT CAATGCCAAC 
AGTACTGCAG GGCAGCCCCC TCCCCCTCCC ATCAAACCCA GCCTCATCCC GACAGGCAAC 
TTTAAAGGAT TTAATTCATA CACTGGAAAC ACAACAGGGG TTGTAGTATC AGGAGAAGTT 
TACAAGGACC TGCGAGAAAA GTGGACGAAA GGTGGTCAAG AACAGGAAAA CACTTTAACT 
GAAGGAGCCC CGGAGAGTAT GACATTCAAG GAGCGCCAGC GGCTCTTTTC CCAAGGGAAG 
GAAGTATCCA ACAAAGTCAA GGCCTCGCGA AAACTCATGG AGCTGGAAAA TGAACTCAAT 
ACAAAGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144425.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999900.1 Salmo salar afadin-like (LOC106571391), transcript variant X8, mRNA.

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CloneID	OAt47157
Clone ID Related Accession (Same CDS sequence)	XM_014144425.1
Accession Version	XM_014144425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGC AGACTGCTGT CCCAGCAATC 
TCTGTTCTAG ACTTGTTGCA GGACGAGGAG CGGCGGAGGA AGCAACAGCT GGAGGAGATC 
CGTAAGCGCG AAGCGGAGGA CCGGGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGGGCCA GACGAGAGGT CGATGAGAAG AGGAGACAGG AGGAGGAATA CTACACACGC 
CTGGAGGAAG GGCGGCGCCG GCAGCACGAG GAGGCCGAGC GCAAGCTGCT GACGCCTGAC 
GAGCCTGGTC TGTACAGACC TCCCCTCCCC AGGGACTACC AGCCTCAGCC CCCCCCTCCC 
ACTGCATCCC CCGCAACCAC TGCCACTAAC GCTCCACCAC CCCCGCCTCA GAGAAACACT 
TCCTACCTCA AAACCCAGGT CGTCTCCCCT GACGCCTTGT ACACTGCCAA GTTTGTCTCG 
TACAATGACG AGGAGGATGA GGACACTACA GGCCTAGCAG GATTTAATTC ATACACTGGA 
AACACAACAG GGGTTGTAGT ATCAGGAGAA GTTTACAAGG ACCTGCGAGA AAAGTGGACG 
AAAGGTGGTC AAGAACAGGA AAACACTTTA ACTGAAGGAG CCCCGGAGAG TATGACATTC 
AAGGAGCGCC AGCGGCTCTT TTCCCAAGGG AAGGAAGTAT CCAACAAAGT CAAGGCCTCG 
CGAAAACTCA TGGAGCTGGA AAATGAACTC AATACAAAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999900.1
CDS	233..5917
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQEIPEADQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGLYRPPLPRDYQPQPPPPTASPATTATNAPPPPPQRNTSYLKTQVVSPDALYTAKFVSYNDEEDEDTTGLAGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144425.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144425.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGC AGACTGCTGT CCCAGCAATC 
TCTGTTCTAG ACTTGTTGCA GGACGAGGAG CGGCGGAGGA AGCAACAGCT GGAGGAGATC 
CGTAAGCGCG AAGCGGAGGA CCGGGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGGGCCA GACGAGAGGT CGATGAGAAG AGGAGACAGG AGGAGGAATA CTACACACGC 
CTGGAGGAAG GGCGGCGCCG GCAGCACGAG GAGGCCGAGC GCAAGCTGCT GACGCCTGAC 
GAGCCTGGTC TGTACAGACC TCCCCTCCCC AGGGACTACC AGCCTCAGCC CCCCCCTCCC 
ACTGCATCCC CCGCAACCAC TGCCACTAAC GCTCCACCAC CCCCGCCTCA GAGAAACACT 
TCCTACCTCA AAACCCAGGT CGTCTCCCCT GACGCCTTGT ACACTGCCAA GTTTGTCTCG 
TACAATGACG AGGAGGATGA GGACACTACA GGCCTAGCAG GATTTAATTC ATACACTGGA 
AACACAACAG GGGTTGTAGT ATCAGGAGAA GTTTACAAGG ACCTGCGAGA AAAGTGGACG 
AAAGGTGGTC AAGAACAGGA AAACACTTTA ACTGAAGGAG CCCCGGAGAG TATGACATTC 
AAGGAGCGCC AGCGGCTCTT TTCCCAAGGG AAGGAAGTAT CCAACAAAGT CAAGGCCTCG 
CGAAAACTCA TGGAGCTGGA AAATGAACTC AATACAAAGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144426.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999901.1 Salmo salar afadin-like (LOC106571391), transcript variant X9, mRNA.

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CloneID	OAt47158
Clone ID Related Accession (Same CDS sequence)	XM_014144426.1
Accession Version	XM_014144426.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGT TGCAGGACGA GGAGCGGCGG 
AGGAAGCAAC AGCTGGAGGA GATCCGTAAG CGCGAAGCGG AGGACCGGGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGG GCCAGACGAG AGGTCGATGA GAAGAGGAGA 
CAGGAGGAGG AATACTACAC ACGCCTGGAG GAAGGGCGGC GCCGGCAGCA CGAGGAGGCC 
GAGCGCAAGC TGCTGACGCC TGACGAGCCT GGTCTGTACA GACCTCCCCT CCCCAGGGAC 
TACCAGCCTC AGCCCCCCCC TCCCACTGCA TCCCCCGCAA CCACTGCCAC TAACGCTCCA 
CCACCCCCGC CTCAGAGAAA CACTTCCTAC CTCAAAACCC AGGTCGTCTC CCCTGACGCC 
TTGTACACTG CCAAGTTTGT CTCGTACAAT GACGAGGAGG ATGAGGACAC TACAGGCCTA 
GCAGGATTTA ATTCATACAC TGGAAACACA ACAGGGGTTG TAGTATCAGG AGAAGTTTAC 
AAGGACCTGC GAGAAAAGTG GACGAAAGGT GGTCAAGAAC AGGAAAACAC TTTAACTGAA 
GGAGCCCCGG AGAGTATGAC ATTCAAGGAG CGCCAGCGGC TCTTTTCCCA AGGGAAGGAA 
GTATCCAACA AAGTCAAGGC CTCGCGAAAA CTCATGGAGC TGGAAAATGA ACTCAATACA 
AAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999901.1
CDS	233..5881
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQEIPEADQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGLYRPPLPRDYQPQPPPPTASPATTATNAPPPPPQRNTSYLKTQVVSPDALYTAKFVSYNDEEDEDTTGLAGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144426.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144426.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGT TGCAGGACGA GGAGCGGCGG 
AGGAAGCAAC AGCTGGAGGA GATCCGTAAG CGCGAAGCGG AGGACCGGGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGG GCCAGACGAG AGGTCGATGA GAAGAGGAGA 
CAGGAGGAGG AATACTACAC ACGCCTGGAG GAAGGGCGGC GCCGGCAGCA CGAGGAGGCC 
GAGCGCAAGC TGCTGACGCC TGACGAGCCT GGTCTGTACA GACCTCCCCT CCCCAGGGAC 
TACCAGCCTC AGCCCCCCCC TCCCACTGCA TCCCCCGCAA CCACTGCCAC TAACGCTCCA 
CCACCCCCGC CTCAGAGAAA CACTTCCTAC CTCAAAACCC AGGTCGTCTC CCCTGACGCC 
TTGTACACTG CCAAGTTTGT CTCGTACAAT GACGAGGAGG ATGAGGACAC TACAGGCCTA 
GCAGGATTTA ATTCATACAC TGGAAACACA ACAGGGGTTG TAGTATCAGG AGAAGTTTAC 
AAGGACCTGC GAGAAAAGTG GACGAAAGGT GGTCAAGAAC AGGAAAACAC TTTAACTGAA 
GGAGCCCCGG AGAGTATGAC ATTCAAGGAG CGCCAGCGGC TCTTTTCCCA AGGGAAGGAA 
GTATCCAACA AAGTCAAGGC CTCGCGAAAA CTCATGGAGC TGGAAAATGA ACTCAATACA 
AAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144427.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999902.1 Salmo salar afadin-like (LOC106571391), transcript variant X10, mRNA.

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CloneID	OAt47159
Clone ID Related Accession (Same CDS sequence)	XM_014144427.1
Accession Version	XM_014144427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGC AGACTGCTGT CCCAGCAATC 
TCTGTTCTAG ACTTGTTGCA GGACGAGGAG CGGCGGAGGA AGCAACAGCT GGAGGAGATC 
CGTAAGCGCG AAGCGGAGGA CCGGGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGGGCCA GACGAGAGGT CGATGAGAAG AGGAGACAGG AGGAGGAATA CTACACACGC 
CTGGAGGAAG GGCGGCGCCG GCAGCACGAG GAGGCCGAGC GCAAGCTGCT GACGCCTGAC 
GAGCCTGGAT TTAATTCATA CACTGGAAAC ACAACAGGGG TTGTAGTATC AGGAGAAGTT 
TACAAGGACC TGCGAGAAAA GTGGACGAAA GGTGGTCAAG AACAGGAAAA CACTTTAACT 
GAAGGAGCCC CGGAGAGTAT GACATTCAAG GAGCGCCAGC GGCTCTTTTC CCAAGGGAAG 
GAAGTATCCA ACAAAGTCAA GGCCTCGCGA AAACTCATGG AGCTGGAAAA TGAACTCAAT 
ACAAAGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999902.1
CDS	233..5704
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQEIPEADQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144427.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144427.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGC AGACTGCTGT CCCAGCAATC 
TCTGTTCTAG ACTTGTTGCA GGACGAGGAG CGGCGGAGGA AGCAACAGCT GGAGGAGATC 
CGTAAGCGCG AAGCGGAGGA CCGGGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGGGCCA GACGAGAGGT CGATGAGAAG AGGAGACAGG AGGAGGAATA CTACACACGC 
CTGGAGGAAG GGCGGCGCCG GCAGCACGAG GAGGCCGAGC GCAAGCTGCT GACGCCTGAC 
GAGCCTGGAT TTAATTCATA CACTGGAAAC ACAACAGGGG TTGTAGTATC AGGAGAAGTT 
TACAAGGACC TGCGAGAAAA GTGGACGAAA GGTGGTCAAG AACAGGAAAA CACTTTAACT 
GAAGGAGCCC CGGAGAGTAT GACATTCAAG GAGCGCCAGC GGCTCTTTTC CCAAGGGAAG 
GAAGTATCCA ACAAAGTCAA GGCCTCGCGA AAACTCATGG AGCTGGAAAA TGAACTCAAT 
ACAAAGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144428.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999903.1 Salmo salar afadin-like (LOC106571391), transcript variant X11, mRNA.

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CloneID	OAt47160
Clone ID Related Accession (Same CDS sequence)	XM_014144428.1
Accession Version	XM_014144428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGT TGCAGGACGA GGAGCGGCGG 
AGGAAGCAAC AGCTGGAGGA GATCCGTAAG CGCGAAGCGG AGGACCGGGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGG GCCAGACGAG AGGTCGATGA GAAGAGGAGA 
CAGGAGGAGG AATACTACAC ACGCCTGGAG GAAGGGCGGC GCCGGCAGCA CGAGGAGGCC 
GAGCGCAAGC TGCTGACGCC TGACGAGCCT GGATTTAATT CATACACTGG AAACACAACA 
GGGGTTGTAG TATCAGGAGA AGTTTACAAG GACCTGCGAG AAAAGTGGAC GAAAGGTGGT 
CAAGAACAGG AAAACACTTT AACTGAAGGA GCCCCGGAGA GTATGACATT CAAGGAGCGC 
CAGCGGCTCT TTTCCCAAGG GAAGGAAGTA TCCAACAAAG TCAAGGCCTC GCGAAAACTC 
ATGGAGCTGG AAAATGAACT CAATACAAAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999903.1
CDS	233..5668
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQEIPEADQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144428.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144428.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGT TGCAGGACGA GGAGCGGCGG 
AGGAAGCAAC AGCTGGAGGA GATCCGTAAG CGCGAAGCGG AGGACCGGGC CAAACAGGAG 
GAGGAGAGAC GCTGGAGAGA GGAGGAGAGG GCCAGACGAG AGGTCGATGA GAAGAGGAGA 
CAGGAGGAGG AATACTACAC ACGCCTGGAG GAAGGGCGGC GCCGGCAGCA CGAGGAGGCC 
GAGCGCAAGC TGCTGACGCC TGACGAGCCT GGATTTAATT CATACACTGG AAACACAACA 
GGGGTTGTAG TATCAGGAGA AGTTTACAAG GACCTGCGAG AAAAGTGGAC GAAAGGTGGT 
CAAGAACAGG AAAACACTTT AACTGAAGGA GCCCCGGAGA GTATGACATT CAAGGAGCGC 
CAGCGGCTCT TTTCCCAAGG GAAGGAAGTA TCCAACAAAG TCAAGGCCTC GCGAAAACTC 
ATGGAGCTGG AAAATGAACT CAATACAAAG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144429.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999904.1 Salmo salar afadin-like (LOC106571391), transcript variant X12, mRNA.

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CloneID	OAt47161
Clone ID Related Accession (Same CDS sequence)	XM_014144429.1
Accession Version	XM_014144429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGC AGACTGCTGT CCCAGCAATC 
TCTGTTCTAG ACTTGTTGCA GGACGAGGAG CGGCGGAGGA AGCAACAGCT GGAGGAGATC 
CGTAAGCGCG AAGCGGAGGA CCGGGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGGGCCA GACGAGAGGT CGATGAGAAG GATTTAATTC ATACACTGGA AACACAACAG 
GGGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999904.1
CDS	234..5402
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQEIPEADQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKDLIHTLETQQGL

Target ORF information:

RefSeq Version	XM_014144429.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144429.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGGAGA TTCCTGAAGC GGACCAGAAG 
CAGAAGAACA TTGCGGGGTC CCTGGCTTTC TGGATGGCCA ATGCCTCAGA GCTGCTCAAC 
TTCATCAAGC AGGACAAGGA CCTGTCGCGC ATCACACTGG ACGCCCAGGA CGTGCTGGCA 
CACCTCGTCC AGATGGCCTT CAAGTACCTG GTCCAGTGTC TGCAGTCTGA CCTGAGTAAC 
TACATGCCTG CCTTCCTGGA CGACCCAGAG GAACAGAACC CTCAAAGGCC CAAAATAGAG 
GATGTTTTGC ACACCCTGAC GGGCGCCATG TCGCTGCTGC GTCGTTGCCG TGTCAACGCG 
GCGCTGACCA TCCAGCTGTT CTCCCAGCTC TTTCACTTCA TCAACATGTG GCTGTTCAAC 
AAGCTGGTGA TGGAGGCTGA CTCTGGACTG TGTTCACACT ACTGGGGCGC CATCCTCCGC 
CAGCAGCTCA GCCACATCGA GGCCTGGGCA GAGAAACAGG GCCTGGAGCT GGCCGCCGAC 
TGCCACCTCA GTCGCATCGT ACAGGCTACG ACTCTCCTCA CCATGGACAA GTACTCCATG 
CAGGACGTGC AGAACATCCA CAACACCTGC TTCAAGCTGA ACTCCCTACA GCTCAACGCC 
CTCATGACCA ACTACCACTG TGCTCCCGAC GAGCCTTACA TCCCCCCAGA GCTCATAGAC 
CACGTGGTTG CCGTGGCGGA GAACACAGCA GATGAGCTGG CCCGTAGCGA CGGCCGAGAG 
GTCCAGCTAG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TACTGCCCGA GGACGGCTAC 
TCCTGCGACG TCGTGAGGAA CATTCCCAAC GGATTCCAGG AGTTCCTGGA CCCACTTTGT 
CAGAGAGGAT TCTGTCGGCT AACCCCTCAC CCGCGCTCCC CTGGGACCTG GACCATCCAC 
TTTGAAGGAG TCGACTGCGA CAGCCACTTC TCTGCAGACA ACTCAGACCT GCAAATGCGG 
AAGGAACCGG AAGTTGTGAC GGTCACCCTG AAGAAGCACA ATGGCATGGG CCTCAGCATT 
GTGGCTGCCA AGGGTGCTGG GCAGGAGAAG CTCGGGATCT ACATCAAGTC TGTTGTGAAG 
GGAGGCGCGG CTGACGTGGA CGGGCGTCTG GCTGCAGGGG ACCAGCTGCT GAGTGTGGAC 
GGCCGCAGCC TGGTGGGCCT CTCCCAGGAG AGGGCTGCGG AGTTGATGAC GAGGACAGGT 
TCTGTGGTAA CACTGGAGGT GGCTAAACAG GGAGCGATCT ACCACGGCCT GGCCACGCTC 
CTCAACCAGC CATCTCCTAT GATGCCCAGA GCGTCGCAAG GAGGGCGTGA CAAGAATGGC 
AAACTGAGAC CAAAGAGCGA AGGCTTTGAA CTGTACAACA ACTCGGTCCA GAACGGCTCT 
CCAGAGAGCC CCCAGGCCGA CTGGCAGGCC TACCCAGAAC CAAAGAAGAT GAGCGGGGAG 
GAGAGGCTCC TGAAGAACCG GGCCGACCAC CGCTCCAGCC CCAACGTGGC CAACCAGAGC 
CAGAGTCCGG GAGGGAAGGC CGTCTACACA CGAGGACCTG GGACCAAGAT CACCTCTGTC 
TCCACGGGCA ACCTGTGTGT TGATGACGAG CCCTCTCCTC CCCATCCGGA GGCCTATCCT 
ATTCCCACTC AGACCTACCC CAGGGAATAC TTCACCATCC CAGCCTCCAA GTCTACGAAC 
CGGATGGACC CGGCCCCAGG ACAGTTATAC GCTGACGACA GGGGTCCTCC CGGCCCCATG 
GAAGACCACC ACCATAACAG CATAGCTATG CAGCGGGTGG ACCACTCTCA GGAGGAGCTG 
TGTGACCTCC CGGGGGGCTA CCCTCCAACC AGCCTGCGTC CTGAGGACCT GGTCCACTTG 
CACCAGCAGC AGCAGAGAGA GGCTGACTAC CAGTGGGGCA GTGGGGAACA CTGGGTCAAC 
CACCACCAGG TCTCCTCTTC CGTGGAGTCC TCCACCTCCA GCCAGGAGCA CCTCAACTAC 
TCCTCGTCCT CCTCCAACAA GACCCAGAGC AACCACAAGA GTGGCCCCGG ACGCTGGAAG 
ACCCCCGGCC CCATTGCCCC CCACCCAGGC CCCGTGGCCC AGTCCACACG CACGGACCTC 
CTTCCCCCTC CACCGCCCCC TCCCCCCGTA CACTACCAGG ACTTTGAGCT CCAGCCCGAC 
CTGCCCCTGC CCCCTCCTCC TTCGGTCACC CAGCAGCAGG CCGCAGCAGC AGCCGCCGAC 
CGCAAGAAGC GCGAGGAGCA GCAGCGCTGG TATGAGAAGG AGAAGGCCCG GTTAGAGGAG 
GAGAGGGAGA GGAAGAGGAG GGAGCAGGAG AGGAAGCTGG GACAGGTCCG TAACCCAGCT 
GTGCCTGTAA CCCACAACAA CAACCAACAC GGGGGCACAC TCCCCCCTCC CACACAGCAC 
CAGCAGGTGC CCCCTCCCCG GTCCCCCATG CAGCAGGTGC CCCCTCCCCG GTCCCCCATG 
CAGCAAGTGC CCCATCCCTA CCTCCCCCCC CAGGGCCAAC AGCAGGCCCA GCAGCAGCAG 
CCCCCCAGGC CAGAGAAACT GTCCACCCTG CCCAGAGCGG GCACCCTGCC CCCTCAGGGT 
AACCCCATGG ACACAGTGAT CAGAGAGCTG CTCCCCCAGC AGCAGCCCCG CACCATCGAG 
CGCCGGGACC TGCAGTACAT CACCATCAGC AAGGAGGAGC TGTCCTCCAG CGACAGCCTG 
TCCCCAGACC CCTGGAAGAG GGATGCCCGG GAGAAGTTGG AGAAGCAGCA GCAGCTGCAC 
ATCGTAGACC TGCTGGACAG GGAGATCCAG GACCTGCAGG CCAAGCCGGA GCGTACGGCC 
GAGGAGAGCG ACCGGCTCAG GAAGTTGATG TTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC ACAGTGATGA GGAAGATGAG GAGGATGACG ACGAGGACGT GGACACCATG 
ATGATCATGC AGCGGCTGGA GGCAGAGAAG AGAGCAAGGC AGACTGCTGT CCCAGCAATC 
TCTGTTCTAG ACTTGTTGCA GGACGAGGAG CGGCGGAGGA AGCAACAGCT GGAGGAGATC 
CGTAAGCGCG AAGCGGAGGA CCGGGCCAAA CAGGAGGAGG AGAGACGCTG GAGAGAGGAG 
GAGAGGGCCA GACGAGAGGT CGATGAGAAG GATTTAATTC ATACACTGGA AACACAACAG 
GGGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144430.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999905.1 Salmo salar afadin-like (LOC106571391), transcript variant X13, mRNA.

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CloneID	OAt47162
Clone ID Related Accession (Same CDS sequence)	XM_014144430.1
Accession Version	XM_014144430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGAAGC AGAAGAACAT TGCGGGGTCC 
CTGGCTTTCT GGATGGCCAA TGCCTCAGAG CTGCTCAACT TCATCAAGCA GGACAAGGAC 
CTGTCGCGCA TCACACTGGA CGCCCAGGAC GTGCTGGCAC ACCTCGTCCA GATGGCCTTC 
AAGTACCTGG TCCAGTGTCT GCAGTCTGAC CTGAGTAACT ACATGCCTGC CTTCCTGGAC 
GACCCAGAGG AACAGAACCC TCAAAGGCCC AAAATAGAGG ATGTTTTGCA CACCCTGACG 
GGCGCCATGT CGCTGCTGCG TCGTTGCCGT GTCAACGCGG CGCTGACCAT CCAGCTGTTC 
TCCCAGCTCT TTCACTTCAT CAACATGTGG CTGTTCAACA AGCTGGTGAT GGAGGCTGAC 
TCTGGACTGT GTTCACACTA CTGGGGCGCC ATCCTCCGCC AGCAGCTCAG CCACATCGAG 
GCCTGGGCAG AGAAACAGGG CCTGGAGCTG GCCGCCGACT GCCACCTCAG TCGCATCGTA 
CAGGCTACGA CTCTCCTCAC CATGGACAAG TACTCCATGC AGGACGTGCA GAACATCCAC 
AACACCTGCT TCAAGCTGAA CTCCCTACAG CTCAACGCCC TCATGACCAA CTACCACTGT 
GCTCCCGACG AGCCTTACAT CCCCCCAGAG CTCATAGACC ACGTGGTTGC CGTGGCGGAG 
AACACAGCAG ATGAGCTGGC CCGTAGCGAC GGCCGAGAGG TCCAGCTAGA GGAGGACCCT 
GACCTGCAGC TGCCCTTCCT ACTGCCCGAG GACGGCTACT CCTGCGACGT CGTGAGGAAC 
ATTCCCAACG GATTCCAGGA GTTCCTGGAC CCACTTTGTC AGAGAGGATT CTGTCGGCTA 
ACCCCTCACC CGCGCTCCCC TGGGACCTGG ACCATCCACT TTGAAGGAGT CGACTGCGAC 
AGCCACTTCT CTGCAGACAA CTCAGACCTG CAAATGCGGA AGGAACCGGA AGTTGTGACG 
GTCACCCTGA AGAAGCACAA TGGCATGGGC CTCAGCATTG TGGCTGCCAA GGGTGCTGGG 
CAGGAGAAGC TCGGGATCTA CATCAAGTCT GTTGTGAAGG GAGGCGCGGC TGACGTGGAC 
GGGCGTCTGG CTGCAGGGGA CCAGCTGCTG AGTGTGGACG GCCGCAGCCT GGTGGGCCTC 
TCCCAGGAGA GGGCTGCGGA GTTGATGACG AGGACAGGTT CTGTGGTAAC ACTGGAGGTG 
GCTAAACAGG GAGCGATCTA CCACGGCCTG GCCACGCTCC TCAACCAGCC ATCTCCTATG 
ATGCCCAGAG CGTCGCAAGG AGGGCGTGAC AAGAATGGCA AACTGAGACC AAAGAGCGAA 
GGCTTTGAAC TGTACAACAA CTCGGTCCAG AACGGCTCTC CAGAGAGCCC CCAGGCCGAC 
TGGCAGGCCT ACCCAGAACC AAAGAAGATG AGCGGGGAGG AGAGGCTCCT GAAGAACCGG 
GCCGACCACC GCTCCAGCCC CAACGTGGCC AACCAGAGCC AGAGTCCGGG AGGGAAGGCC 
GTCTACACAC GAGGACCTGG GACCAAGATC ACCTCTGTCT CCACGGGCAA CCTGTGTGTT 
GATGACGAGC CCTCTCCTCC CCATCCGGAG GCCTATCCTA TTCCCACTCA GACCTACCCC 
AGGGAATACT TCACCATCCC AGCCTCCAAG TCTACGAACC GGATGGACCC GGCCCCAGGA 
CAGTTATACG CTGACGACAG GGGTCCTCCC GGCCCCATGG AAGACCACCA CCATAACAGC 
ATAGCTATGC AGCGGGTGGA CCACTCTCAG GAGGAGCTGT GTGACCTCCC GGGGGGCTAC 
CCTCCAACCA GCCTGCGTCC TGAGGACCTG GTCCACTTGC ACCAGCAGCA GCAGAGAGAG 
GCTGACTACC AGTGGGGCAG TGGGGAACAC TGGGTCAACC ACCACCAGGT CTCCTCTTCC 
GTGGAGTCCT CCACCTCCAG CCAGGAGCAC CTCAACTACT CCTCGTCCTC CTCCAACAAG 
ACCCAGAGCA ACCACAAGAG TGGCCCCGGA CGCTGGAAGA CCCCCGGCCC CATTGCCCCC 
CACCCAGGCC CCGTGGCCCA GTCCACACGC ACGGACCTCC TTCCCCCTCC ACCGCCCCCT 
CCCCCCGTAC ACTACCAGGA CTTTGAGCTC CAGCCCGACC TGCCCCTGCC CCCTCCTCCT 
TCGGTCACCC AGCAGCAGGC CGCAGCAGCA GCCGCCGACC GCAAGAAGCG CGAGGAGCAG 
CAGCGCTGGT ATGAGAAGGA GAAGGCCCGG TTAGAGGAGG AGAGGGAGAG GAAGAGGAGG 
GAGCAGGAGA GGAAGCTGGG ACAGGTCCGT AACCCAGCTG TGCCTGTAAC CCACAACAAC 
AACCAACACG GGGGCACACT CCCCCCTCCC ACACAGCACC AGCAGGTGCC CCCTCCCCGG 
TCCCCCATGC AGCAGGTGCC CCCTCCCCGG TCCCCCATGC AGCAAGTGCC CCATCCCTAC 
CTCCCCCCCC AGGGCCAACA GCAGGCCCAG CAGCAGCAGC CCCCCAGGCC AGAGAAACTG 
TCCACCCTGC CCAGAGCGGG CACCCTGCCC CCTCAGGGTA ACCCCATGGA CACAGTGATC 
AGAGAGCTGC TCCCCCAGCA GCAGCCCCGC ACCATCGAGC GCCGGGACCT GCAGTACATC 
ACCATCAGCA AGGAGGAGCT GTCCTCCAGC GACAGCCTGT CCCCAGACCC CTGGAAGAGG 
GATGCCCGGG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTAGACCT GCTGGACAGG 
GAGATCCAGG ACCTGCAGGC CAAGCCGGAG CGTACGGCCG AGGAGAGCGA CCGGCTCAGG 
AAGTTGATGT TGGAGTGGCA GTTCCAGAAG AGACTGCAGG AGTCCAAGCA CAGTGATGAG 
GAAGATGAGG AGGATGACGA CGAGGACGTG GACACCATGA TGATCATGCA GCGGCTGGAG 
GCAGAGAAGA GAGCAAGGCA GACTGCTGTC CCAGCAATCT CTGTTCTAGA CTTGTTGCAG 
GACGAGGAGC GGCGGAGGAA GCAACAGCTG GAGGAGATCC GTAAGCGCGA AGCGGAGGAC 
CGGGCCAAAC AGGAGGAGGA GAGACGCTGG AGAGAGGAGG AGAGGGCCAG ACGAGAGGTC 
GATGAGAAGA GGAGACAGGA GGAGGAATAC TACACACGCC TGGAGGAAGG GCGGCGCCGG 
CAGCACGAGG AGGCCGAGCG CAAGCTGCTG ACGCCTGACG AGCCTGGTCT GTACAGACCT 
CCCCTCCCCA GGGACTACCA GCCTCAGCCC CCCCCTCCCA CTGCATCCCC CGCAACCACT 
GCCACTAACG CTCCACCACC CCCGCCTCAG AGAAACACTT CCTACCTCAA AACCCAGGTC 
GTCTCCCCTG ACGCCTTGTA CACTGCCAAG TTTGTCTCGT ACAATGACGA GGAGGATGAG 
GACACTACAG GCCTAGCAGG ATTTAATTCA TACACTGGAA ACACAACAGG GGTTGTAGTA 
TCAGGAGAAG TTTACAAGGA CCTGCGAGAA AAGTGGACGA AAGGTGGTCA AGAACAGGAA 
AACACTTTAA CTGAAGGAGC CCCGGAGAGT ATGACATTCA AGGAGCGCCA GCGGCTCTTT 
TCCCAAGGGA AGGAAGTATC CAACAAAGTC AAGGCCTCGC GAAAACTCAT GGAGCTGGAA 
AATGAACTCA ATACAAAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999905.1
CDS	233..5896
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGLYRPPLPRDYQPQPPPPTASPATTATNAPPPPPQRNTSYLKTQVVSPDALYTAKFVSYNDEEDEDTTGLAGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144430.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144430.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
GAACGGAAGC TGGACCTGGA TGAGAAGCCA CTGGTGGTGC AGCTCAACTG GAACAAAGAC 
GACCGCGAGG GACGTTTCGT CCTCAAGAAT GAGAACGACA TCATCCCCAA GAAGGCGCAG 
AGCAACGGGC CCGAGAGGGA GAAGGAGGGA GTGATCCAGA ACTTCAAACG GACACTCTCC 
AAGAAAGAGA AGAAGAAGGA GAAGAAGCGA GAGAAGGAGG CGCACATTGC TGACGGAGAT 
GAGCAAGCAC TGAGCAGAGA AGATGGTGAG AACTCCAGAC TGGCAGCGGA GGTGTACAAG 
GACATGCCAG AGAACAGCTT CACCAGGACC ATCTCCAACC CTGAGGTGGT GATGAAGAGG 
AGGCGGCAGC AGAAACTGGA GAAGCGGATG CAGGAGTTCC GCAGCAGCGA CGGCAGGCCA 
GACTCGGGGG GGACGTTGAG GATCTATGCA GACAGTCTGA AACCCAATAT CCCCTATAAG 
ACCATCTTGC TGTCCACGAC AGACATGGCA GATTTTGCTG TGGTGGAGGC CCTGGAGAAG 
TACGGCCTGG AGAAGGAGAA CCCCCGGGAG TACTGCATCG CCAGGCACAC AGATGACAAG 
TCTGGGAAGG AGATCATCCT GGACGATACA GAGTGTCCTC TTCAGATCTT CAGAGACTGG 
CCCAACGACA GAGGGGACCT AGTGTTCCAG CTGAAGAAGA GACCTCCAGA CTACGTGCCA 
AAGAAGGGCC GCAAGCCAGA GGACAAGGGC CCCAGGGGGA AGGGCATGGA CGTGCCCCTG 
CATGGGTCTC TACCCCCAGA GAAACTGCCT TACCTGGTGG AGCTCAGCCC AGGGAGGGGG 
AATCACTATG GCTACTACAC CTATCGGCAC CACGAAGACG GCTCAGACTC CAGGGACAAA 
CCGAAGCTCT ACCGTCTGCA GCACAGTGTG ACTGAGGTGG GCTCAGACAC GACAGATGAC 
GGCTCCATCC AGCTGTTTGG TCCAGGTATC CTGCCCCACC ACTGTAAACT GATGCACACG 
GAGGGTTTGG TGACAGTGAC ACCACACAGT CTGGAGGCTG AGACCTACGT GGACGGCCAG 
CGTGTCGCCG AGACCACCGT GCTGCGAAGC GGAGCCACAC TGCAGTTCGG AGCCTCCCAT 
GTCTTCAAGT TTGTGGACCC CAGCTACGAC CACAGCATGG GCAAGGGAGG GCCCGGACCA 
ATGATGAGAG GAAGGCACAA GTCAGGAAGT GTTCCTGAGG CGACCTTTGA CCTGCAAGGG 
GACCTCCACA GTGGGACTGC CCTCTCCACC AGCAAGAGCT CAGGGAAGCT GGAAATGGAG 
CGTTCATCCA ACGGCAGCGA GAGGGGCATG GTCAAACCCA TGATCCGCGG GGAGCAGCAG 
GACAGCAGGT CCCAGGACAG CCTGAGCCCA GAAGTGACGC TGCCGGCCAG CATCGAGTTC 
AGGGACAACT CTGAAGATAC CTTCCTGACG GCCATCATCA ACTACACCAA CAGCTCCACG 
GTGCACTTCA AGCTGTCCCC CACCTACGTA CTGTACATGA CCTGTCGCTA CGTCATGTCC 
AGCCACTACA GGCCCGACAT CGGTCCCTCG GAGCGCACCC ACAAGGTCAT AGCCATCGTC 
AACAAGATGG TGAGCATGAT GGAAGGAGTC ATCCAGAAGC AGAAGAACAT TGCGGGGTCC 
CTGGCTTTCT GGATGGCCAA TGCCTCAGAG CTGCTCAACT TCATCAAGCA GGACAAGGAC 
CTGTCGCGCA TCACACTGGA CGCCCAGGAC GTGCTGGCAC ACCTCGTCCA GATGGCCTTC 
AAGTACCTGG TCCAGTGTCT GCAGTCTGAC CTGAGTAACT ACATGCCTGC CTTCCTGGAC 
GACCCAGAGG AACAGAACCC TCAAAGGCCC AAAATAGAGG ATGTTTTGCA CACCCTGACG 
GGCGCCATGT CGCTGCTGCG TCGTTGCCGT GTCAACGCGG CGCTGACCAT CCAGCTGTTC 
TCCCAGCTCT TTCACTTCAT CAACATGTGG CTGTTCAACA AGCTGGTGAT GGAGGCTGAC 
TCTGGACTGT GTTCACACTA CTGGGGCGCC ATCCTCCGCC AGCAGCTCAG CCACATCGAG 
GCCTGGGCAG AGAAACAGGG CCTGGAGCTG GCCGCCGACT GCCACCTCAG TCGCATCGTA 
CAGGCTACGA CTCTCCTCAC CATGGACAAG TACTCCATGC AGGACGTGCA GAACATCCAC 
AACACCTGCT TCAAGCTGAA CTCCCTACAG CTCAACGCCC TCATGACCAA CTACCACTGT 
GCTCCCGACG AGCCTTACAT CCCCCCAGAG CTCATAGACC ACGTGGTTGC CGTGGCGGAG 
AACACAGCAG ATGAGCTGGC CCGTAGCGAC GGCCGAGAGG TCCAGCTAGA GGAGGACCCT 
GACCTGCAGC TGCCCTTCCT ACTGCCCGAG GACGGCTACT CCTGCGACGT CGTGAGGAAC 
ATTCCCAACG GATTCCAGGA GTTCCTGGAC CCACTTTGTC AGAGAGGATT CTGTCGGCTA 
ACCCCTCACC CGCGCTCCCC TGGGACCTGG ACCATCCACT TTGAAGGAGT CGACTGCGAC 
AGCCACTTCT CTGCAGACAA CTCAGACCTG CAAATGCGGA AGGAACCGGA AGTTGTGACG 
GTCACCCTGA AGAAGCACAA TGGCATGGGC CTCAGCATTG TGGCTGCCAA GGGTGCTGGG 
CAGGAGAAGC TCGGGATCTA CATCAAGTCT GTTGTGAAGG GAGGCGCGGC TGACGTGGAC 
GGGCGTCTGG CTGCAGGGGA CCAGCTGCTG AGTGTGGACG GCCGCAGCCT GGTGGGCCTC 
TCCCAGGAGA GGGCTGCGGA GTTGATGACG AGGACAGGTT CTGTGGTAAC ACTGGAGGTG 
GCTAAACAGG GAGCGATCTA CCACGGCCTG GCCACGCTCC TCAACCAGCC ATCTCCTATG 
ATGCCCAGAG CGTCGCAAGG AGGGCGTGAC AAGAATGGCA AACTGAGACC AAAGAGCGAA 
GGCTTTGAAC TGTACAACAA CTCGGTCCAG AACGGCTCTC CAGAGAGCCC CCAGGCCGAC 
TGGCAGGCCT ACCCAGAACC AAAGAAGATG AGCGGGGAGG AGAGGCTCCT GAAGAACCGG 
GCCGACCACC GCTCCAGCCC CAACGTGGCC AACCAGAGCC AGAGTCCGGG AGGGAAGGCC 
GTCTACACAC GAGGACCTGG GACCAAGATC ACCTCTGTCT CCACGGGCAA CCTGTGTGTT 
GATGACGAGC CCTCTCCTCC CCATCCGGAG GCCTATCCTA TTCCCACTCA GACCTACCCC 
AGGGAATACT TCACCATCCC AGCCTCCAAG TCTACGAACC GGATGGACCC GGCCCCAGGA 
CAGTTATACG CTGACGACAG GGGTCCTCCC GGCCCCATGG AAGACCACCA CCATAACAGC 
ATAGCTATGC AGCGGGTGGA CCACTCTCAG GAGGAGCTGT GTGACCTCCC GGGGGGCTAC 
CCTCCAACCA GCCTGCGTCC TGAGGACCTG GTCCACTTGC ACCAGCAGCA GCAGAGAGAG 
GCTGACTACC AGTGGGGCAG TGGGGAACAC TGGGTCAACC ACCACCAGGT CTCCTCTTCC 
GTGGAGTCCT CCACCTCCAG CCAGGAGCAC CTCAACTACT CCTCGTCCTC CTCCAACAAG 
ACCCAGAGCA ACCACAAGAG TGGCCCCGGA CGCTGGAAGA CCCCCGGCCC CATTGCCCCC 
CACCCAGGCC CCGTGGCCCA GTCCACACGC ACGGACCTCC TTCCCCCTCC ACCGCCCCCT 
CCCCCCGTAC ACTACCAGGA CTTTGAGCTC CAGCCCGACC TGCCCCTGCC CCCTCCTCCT 
TCGGTCACCC AGCAGCAGGC CGCAGCAGCA GCCGCCGACC GCAAGAAGCG CGAGGAGCAG 
CAGCGCTGGT ATGAGAAGGA GAAGGCCCGG TTAGAGGAGG AGAGGGAGAG GAAGAGGAGG 
GAGCAGGAGA GGAAGCTGGG ACAGGTCCGT AACCCAGCTG TGCCTGTAAC CCACAACAAC 
AACCAACACG GGGGCACACT CCCCCCTCCC ACACAGCACC AGCAGGTGCC CCCTCCCCGG 
TCCCCCATGC AGCAGGTGCC CCCTCCCCGG TCCCCCATGC AGCAAGTGCC CCATCCCTAC 
CTCCCCCCCC AGGGCCAACA GCAGGCCCAG CAGCAGCAGC CCCCCAGGCC AGAGAAACTG 
TCCACCCTGC CCAGAGCGGG CACCCTGCCC CCTCAGGGTA ACCCCATGGA CACAGTGATC 
AGAGAGCTGC TCCCCCAGCA GCAGCCCCGC ACCATCGAGC GCCGGGACCT GCAGTACATC 
ACCATCAGCA AGGAGGAGCT GTCCTCCAGC GACAGCCTGT CCCCAGACCC CTGGAAGAGG 
GATGCCCGGG AGAAGTTGGA GAAGCAGCAG CAGCTGCACA TCGTAGACCT GCTGGACAGG 
GAGATCCAGG ACCTGCAGGC CAAGCCGGAG CGTACGGCCG AGGAGAGCGA CCGGCTCAGG 
AAGTTGATGT TGGAGTGGCA GTTCCAGAAG AGACTGCAGG AGTCCAAGCA CAGTGATGAG 
GAAGATGAGG AGGATGACGA CGAGGACGTG GACACCATGA TGATCATGCA GCGGCTGGAG 
GCAGAGAAGA GAGCAAGGCA GACTGCTGTC CCAGCAATCT CTGTTCTAGA CTTGTTGCAG 
GACGAGGAGC GGCGGAGGAA GCAACAGCTG GAGGAGATCC GTAAGCGCGA AGCGGAGGAC 
CGGGCCAAAC AGGAGGAGGA GAGACGCTGG AGAGAGGAGG AGAGGGCCAG ACGAGAGGTC 
GATGAGAAGA GGAGACAGGA GGAGGAATAC TACACACGCC TGGAGGAAGG GCGGCGCCGG 
CAGCACGAGG AGGCCGAGCG CAAGCTGCTG ACGCCTGACG AGCCTGGTCT GTACAGACCT 
CCCCTCCCCA GGGACTACCA GCCTCAGCCC CCCCCTCCCA CTGCATCCCC CGCAACCACT 
GCCACTAACG CTCCACCACC CCCGCCTCAG AGAAACACTT CCTACCTCAA AACCCAGGTC 
GTCTCCCCTG ACGCCTTGTA CACTGCCAAG TTTGTCTCGT ACAATGACGA GGAGGATGAG 
GACACTACAG GCCTAGCAGG ATTTAATTCA TACACTGGAA ACACAACAGG GGTTGTAGTA 
TCAGGAGAAG TTTACAAGGA CCTGCGAGAA AAGTGGACGA AAGGTGGTCA AGAACAGGAA 
AACACTTTAA CTGAAGGAGC CCCGGAGAGT ATGACATTCA AGGAGCGCCA GCGGCTCTTT 
TCCCAAGGGA AGGAAGTATC CAACAAAGTC AAGGCCTCGC GAAAACTCAT GGAGCTGGAA 
AATGAACTCA ATACAAAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106571391 ( XM_014144431.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013999906.1 Salmo salar afadin-like (LOC106571391), transcript variant X14, mRNA.

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CloneID	OAt47163
Clone ID Related Accession (Same CDS sequence)	XM_014144431.1
Accession Version	XM_014144431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	afadin-like isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012338267.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
CGGAAGCTGG ACCTGGATGA GAAGCCACTG GTGGTGCAGC TCAACTGGAA CAAAGACGAC 
CGCGAGGGAC GTTTCGTCCT CAAGAATGAG AACGACATCA TCCCCAAGAA GGCGCAGAGC 
AACGGGCCCG AGAGGGAGAA GGAGGGAGTG ATCCAGAACT TCAAACGGAC ACTCTCCAAG 
AAAGAGAAGA AGAAGGAGAA GAAGCGAGAG AAGGAGGCGC ACATTGCTGA CGGAGATGAG 
CAAGCACTGA GCAGAGAAGA TGGTGAGAAC TCCAGACTGG CAGCGGAGGT GTACAAGGAC 
ATGCCAGAGA ACAGCTTCAC CAGGACCATC TCCAACCCTG AGGTGGTGAT GAAGAGGAGG 
CGGCAGCAGA AACTGGAGAA GCGGATGCAG GAGTTCCGCA GCAGCGACGG CAGGCCAGAC 
TCGGGGGGGA CGTTGAGGAT CTATGCAGAC AGTCTGAAAC CCAATATCCC CTATAAGACC 
ATCTTGCTGT CCACGACAGA CATGGCAGAT TTTGCTGTGG TGGAGGCCCT GGAGAAGTAC 
GGCCTGGAGA AGGAGAACCC CCGGGAGTAC TGCATCGCCA GGCACACAGA TGACAAGTCT 
GGGAAGGAGA TCATCCTGGA CGATACAGAG TGTCCTCTTC AGATCTTCAG AGACTGGCCC 
AACGACAGAG GGGACCTAGT GTTCCAGCTG AAGAAGAGAC CTCCAGACTA CGTGCCAAAG 
AAGGGCCGCA AGCCAGAGGA CAAGGGCCCC AGGGGGAAGG GCATGGACGT GCCCCTGCAT 
GGGTCTCTAC CCCCAGAGAA ACTGCCTTAC CTGGTGGAGC TCAGCCCAGG GAGGGGGAAT 
CACTATGGCT ACTACACCTA TCGGCACCAC GAAGACGGCT CAGACTCCAG GGACAAACCG 
AAGCTCTACC GTCTGCAGCA CAGTGTGACT GAGGTGGGCT CAGACACGAC AGATGACGGC 
TCCATCCAGC TGTTTGGTCC AGGTATCCTG CCCCACCACT GTAAACTGAT GCACACGGAG 
GGTTTGGTGA CAGTGACACC ACACAGTCTG GAGGCTGAGA CCTACGTGGA CGGCCAGCGT 
GTCGCCGAGA CCACCGTGCT GCGAAGCGGA GCCACACTGC AGTTCGGAGC CTCCCATGTC 
TTCAAGTTTG TGGACCCCAG CTACGACCAC AGCATGGGCA AGGGAGGGCC CGGACCAATG 
ATGAGAGGAA GGCACAAGTC AGGAAGTGTT CCTGAGGCGA CCTTTGACCT GCAAGGGGAC 
CTCCACAGTG GGACTGCCCT CTCCACCAGC AAGAGCTCAG GGAAGCTGGA AATGGAGCGT 
TCATCCAACG GCAGCGAGAG GGGCATGGTC AAACCCATGA TCCGCGGGGA GCAGCAGGAC 
AGCAGGTCCC AGGACAGCCT GAGCCCAGAA GTGACGCTGC CGGCCAGCAT CGAGTTCAGG 
GACAACTCTG AAGATACCTT CCTGACGGCC ATCATCAACT ACACCAACAG CTCCACGGTG 
CACTTCAAGC TGTCCCCCAC CTACGTACTG TACATGACCT GTCGCTACGT CATGTCCAGC 
CACTACAGGC CCGACATCGG TCCCTCGGAG CGCACCCACA AGGTCATAGC CATCGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGAAGCAGA AGAACATTGC GGGGTCCCTG 
GCTTTCTGGA TGGCCAATGC CTCAGAGCTG CTCAACTTCA TCAAGCAGGA CAAGGACCTG 
TCGCGCATCA CACTGGACGC CCAGGACGTG CTGGCACACC TCGTCCAGAT GGCCTTCAAG 
TACCTGGTCC AGTGTCTGCA GTCTGACCTG AGTAACTACA TGCCTGCCTT CCTGGACGAC 
CCAGAGGAAC AGAACCCTCA AAGGCCCAAA ATAGAGGATG TTTTGCACAC CCTGACGGGC 
GCCATGTCGC TGCTGCGTCG TTGCCGTGTC AACGCGGCGC TGACCATCCA GCTGTTCTCC 
CAGCTCTTTC ACTTCATCAA CATGTGGCTG TTCAACAAGC TGGTGATGGA GGCTGACTCT 
GGACTGTGTT CACACTACTG GGGCGCCATC CTCCGCCAGC AGCTCAGCCA CATCGAGGCC 
TGGGCAGAGA AACAGGGCCT GGAGCTGGCC GCCGACTGCC ACCTCAGTCG CATCGTACAG 
GCTACGACTC TCCTCACCAT GGACAAGTAC TCCATGCAGG ACGTGCAGAA CATCCACAAC 
ACCTGCTTCA AGCTGAACTC CCTACAGCTC AACGCCCTCA TGACCAACTA CCACTGTGCT 
CCCGACGAGC CTTACATCCC CCCAGAGCTC ATAGACCACG TGGTTGCCGT GGCGGAGAAC 
ACAGCAGATG AGCTGGCCCG TAGCGACGGC CGAGAGGTCC AGCTAGAGGA GGACCCTGAC 
CTGCAGCTGC CCTTCCTACT GCCCGAGGAC GGCTACTCCT GCGACGTCGT GAGGAACATT 
CCCAACGGAT TCCAGGAGTT CCTGGACCCA CTTTGTCAGA GAGGATTCTG TCGGCTAACC 
CCTCACCCGC GCTCCCCTGG GACCTGGACC ATCCACTTTG AAGGAGTCGA CTGCGACAGC 
CACTTCTCTG CAGACAACTC AGACCTGCAA ATGCGGAAGG AACCGGAAGT TGTGACGGTC 
ACCCTGAAGA AGCACAATGG CATGGGCCTC AGCATTGTGG CTGCCAAGGG TGCTGGGCAG 
GAGAAGCTCG GGATCTACAT CAAGTCTGTT GTGAAGGGAG GCGCGGCTGA CGTGGACGGG 
CGTCTGGCTG CAGGGGACCA GCTGCTGAGT GTGGACGGCC GCAGCCTGGT GGGCCTCTCC 
CAGGAGAGGG CTGCGGAGTT GATGACGAGG ACAGGTTCTG TGGTAACACT GGAGGTGGCT 
AAACAGGGAG CGATCTACCA CGGCCTGGCC ACGCTCCTCA ACCAGCCATC TCCTATGATG 
CCCAGAGCGT CGCAAGGAGG GCGTGACAAG AATGGCAAAC TGAGACCAAA GAGCGAAGGC 
TTTGAACTGT ACAACAACTC GGTCCAGAAC GGCTCTCCAG AGAGCCCCCA GGCCGACTGG 
CAGGCCTACC CAGAACCAAA GAAGATGAGC GGGGAGGAGA GGCTCCTGAA GAACCGGGCC 
GACCACCGCT CCAGCCCCAA CGTGGCCAAC CAGAGCCAGA GTCCGGGAGG GAAGGCCGTC 
TACACACGAG GACCTGGGAC CAAGATCACC TCTGTCTCCA CGGGCAACCT GTGTGTTGAT 
GACGAGCCCT CTCCTCCCCA TCCGGAGGCC TATCCTATTC CCACTCAGAC CTACCCCAGG 
GAATACTTCA CCATCCCAGC CTCCAAGTCT ACGAACCGGA TGGACCCGGC CCCAGGACAG 
TTATACGCTG ACGACAGGGG TCCTCCCGGC CCCATGGAAG ACCACCACCA TAACAGCATA 
GCTATGCAGC GGGTGGACCA CTCTCAGGAG GAGCTGTGTG ACCTCCCGGG GGGCTACCCT 
CCAACCAGCC TGCGTCCTGA GGACCTGGTC CACTTGCACC AGCAGCAGCA GAGAGAGGCT 
GACTACCAGT GGGGCAGTGG GGAACACTGG GTCAACCACC ACCAGGTCTC CTCTTCCGTG 
GAGTCCTCCA CCTCCAGCCA GGAGCACCTC AACTACTCCT CGTCCTCCTC CAACAAGACC 
CAGAGCAACC ACAAGAGTGG CCCCGGACGC TGGAAGACCC CCGGCCCCAT TGCCCCCCAC 
CCAGGCCCCG TGGCCCAGTC CACACGCACG GACCTCCTTC CCCCTCCACC GCCCCCTCCC 
CCCGTACACT ACCAGGACTT TGAGCTCCAG CCCGACCTGC CCCTGCCCCC TCCTCCTTCG 
GTCACCCAGC AGCAGGCCGC AGCAGCAGCC GCCGACCGCA AGAAGCGCGA GGAGCAGCAG 
CGCTGGTATG AGAAGGAGAA GGCCCGGTTA GAGGAGGAGA GGGAGAGGAA GAGGAGGGAG 
CAGGAGAGGA AGCTGGGACA GGTCCGTAAC CCAGCTGTGC CTGTAACCCA CAACAACAAC 
CAACACGGGG GCACACTCCC CCCTCCCACA CAGCACCAGC AGGTGCCCCC TCCCCGGTCC 
CCCATGCAGC AGGTGCCCCC TCCCCGGTCC CCCATGCAGC AAGTGCCCCA TCCCTACCTC 
CCCCCCCAGG GCCAACAGCA GGCCCAGCAG CAGCAGCCCC CCAGGCCAGA GAAACTGTCC 
ACCCTGCCCA GAGCGGGCAC CCTGCCCCCT CAGGGTAACC CCATGGACAC AGTGATCAGA 
GAGCTGCTCC CCCAGCAGCA GCCCCGCACC ATCGAGCGCC GGGACCTGCA GTACATCACC 
ATCAGCAAGG AGGAGCTGTC CTCCAGCGAC AGCCTGTCCC CAGACCCCTG GAAGAGGGAT 
GCCCGGGAGA AGTTGGAGAA GCAGCAGCAG CTGCACATCG TAGACCTGCT GGACAGGGAG 
ATCCAGGACC TGCAGGCCAA GCCGGAGCGT ACGGCCGAGG AGAGCGACCG GCTCAGGAAG 
TTGATGTTGG AGTGGCAGTT CCAGAAGAGA CTGCAGGAGT CCAAGCACAG TGATGAGGAA 
GATGAGGAGG ATGACGACGA GGACGTGGAC ACCATGATGA TCATGCAGCG GCTGGAGGCA 
GAGAAGAGAG CAAGGCAGAC TGCTGTCCCA GCAATCTCTG TTCTAGACTT GTTGCAGGAC 
GAGGAGCGGC GGAGGAAGCA ACAGCTGGAG GAGATCCGTA AGCGCGAAGC GGAGGACCGG 
GCCAAACAGG AGGAGGAGAG ACGCTGGAGA GAGGAGGAGA GGGCCAGACG AGAGGTCGAT 
GAGAAGAGGA GACAGGAGGA GGAATACTAC ACACGCCTGG AGGAAGGGCG GCGCCGGCAG 
CACGAGGAGG CCGAGCGCAA GCTGCTGACG CCTGACGAGC CTGGTCTGTA CAGACCTCCC 
CTCCCCAGGG ACTACCAGCC TCAGCCCCCC CCTCCCACTG CATCCCCCGC AACCACTGCC 
ACTAACGCTC CACCACCCCC GCCTCAGAGA AACACTTCCT ACCTCAAAAC CCAGGTCGTC 
TCCCCTGACG CCTTGTACAC TGCCAAGTTT GTCTCGTACA ATGACGAGGA GGATGAGGAC 
ACTACAGGCC TAGCAGGTCA GGTTAAGCTC TCCTCCACCA GGAAGTCCTA TGGCGACCTC 
CCGCCCGCGC ACAAGCCGCA GCCGCCTGCG CGCAAGCCCC GTCCTATGTC TGATGGGATC 
TTTCTGTCCA GCTCATTCCA GCCGCCGGCT GTCAATGCCA ACAGTACTGC AGGGCAGCCC 
CCTCCCCCTC CCATCAAACC CAGCCTCATC CCGACAGGCA ACTTTAAAGG ATTTAATTCA 
TACACTGGAA ACACAACAGG GGTTGTAGTA TCAGGAGAAG TTTACAAGGA CCTGCGAGAA 
AAGTGGACGA AAGGTGGTCA AGAACAGGAA AACACTTTAA CTGAAGGAGC CCCGGAGAGT 
ATGACATTCA AGGAGCGCCA GCGGCTCTTT TCCCAAGGGA AGGAAGTATC CAACAAAGTC 
AAGGCCTCGC GAAAACTCAT GGAGCTGGAA AATGAACTCA ATACAAAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013999906.1
CDS	233..6106
Translation	MSGSREEERRKLADIINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEREKEGVIQNFKRTLSKKEKKKEKKREKEAHIADGDEQALSREDGENSRLAAEVYKDMPENSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVVEALEKYGLEKENPREYCIARHTDDKSGKEIILDDTECPLQIFRDWPNDRGDLVFQLKKRPPDYVPKKGRKPEDKGPRGKGMDVPLHGSLPPEKLPYLVELSPGRGNHYGYYTYRHHEDGSDSRDKPKLYRLQHSVTEVGSDTTDDGSIQLFGPGILPHHCKLMHTEGLVTVTPHSLEAETYVDGQRVAETTVLRSGATLQFGASHVFKFVDPSYDHSMGKGGPGPMMRGRHKSGSVPEATFDLQGDLHSGTALSTSKSSGKLEMERSSNGSERGMVKPMIRGEQQDSRSQDSLSPEVTLPASIEFRDNSEDTFLTAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSHYRPDIGPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGSLAFWMANASELLNFIKQDKDLSRITLDAQDVLAHLVQMAFKYLVQCLQSDLSNYMPAFLDDPEEQNPQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVMEADSGLCSHYWGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNIHNTCFKLNSLQLNALMTNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGFQEFLDPLCQRGFCRLTPHPRSPGTWTIHFEGVDCDSHFSADNSDLQMRKEPEVVTVTLKKHNGMGLSIVAAKGAGQEKLGIYIKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTGSVVTLEVAKQGAIYHGLATLLNQPSPMMPRASQGGRDKNGKLRPKSEGFELYNNSVQNGSPESPQADWQAYPEPKKMSGEERLLKNRADHRSSPNVANQSQSPGGKAVYTRGPGTKITSVSTGNLCVDDEPSPPHPEAYPIPTQTYPREYFTIPASKSTNRMDPAPGQLYADDRGPPGPMEDHHHNSIAMQRVDHSQEELCDLPGGYPPTSLRPEDLVHLHQQQQREADYQWGSGEHWVNHHQVSSSVESSTSSQEHLNYSSSSSNKTQSNHKSGPGRWKTPGPIAPHPGPVAQSTRTDLLPPPPPPPPVHYQDFELQPDLPLPPPPSVTQQQAAAAAADRKKREEQQRWYEKEKARLEEERERKRREQERKLGQVRNPAVPVTHNNNQHGGTLPPPTQHQQVPPPRSPMQQVPPPRSPMQQVPHPYLPPQGQQQAQQQQPPRPEKLSTLPRAGTLPPQGNPMDTVIRELLPQQQPRTIERRDLQYITISKEELSSSDSLSPDPWKRDAREKLEKQQQLHIVDLLDREIQDLQAKPERTAEESDRLRKLMLEWQFQKRLQESKHSDEEDEEDDDEDVDTMMIMQRLEAEKRARQTAVPAISVLDLLQDEERRRKQQLEEIRKREAEDRAKQEEERRWREEERARREVDEKRRQEEEYYTRLEEGRRRQHEEAERKLLTPDEPGLYRPPLPRDYQPQPPPPTASPATTATNAPPPPPQRNTSYLKTQVVSPDALYTAKFVSYNDEEDEDTTGLAGQVKLSSTRKSYGDLPPAHKPQPPARKPRPMSDGIFLSSSFQPPAVNANSTAGQPPPPPIKPSLIPTGNFKGFNSYTGNTTGVVVSGEVYKDLREKWTKGGQEQENTLTEGAPESMTFKERQRLFSQGKEVSNKVKASRKLMELENELNTKQ

Target ORF information:

RefSeq Version	XM_014144431.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar afadin-like (LOC106571391), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014144431.1

ORF Insert Sequence:

ATGTCGGGGA GCAGGGAGGA GGAGCGGCGG AAACTAGCCG ACATCATTAA CCACTGGAAC 
GCCAACCGAC TTGACCTGTT CGAGATCAGC CAACCCACTG AGGACTTGGA GTTCCATGGC 
GTGATGCGTT TCTACTTCCA GGACCGCGTA GCAGGGAACT TTGCCACCAA GTGTATCCGC 
GTGTCCTCCA CGGCCACCAC TCAGGATGTC ATCGAGACGC TGGCCGAGAA GTTCCGGCCC 
GACATGAGGA TGCTCTCCTC GCCCAAATAC TCCCTCTACG AGGTCCATGT CAGCGGGGAA 
CGGAAGCTGG ACCTGGATGA GAAGCCACTG GTGGTGCAGC TCAACTGGAA CAAAGACGAC 
CGCGAGGGAC GTTTCGTCCT CAAGAATGAG AACGACATCA TCCCCAAGAA GGCGCAGAGC 
AACGGGCCCG AGAGGGAGAA GGAGGGAGTG ATCCAGAACT TCAAACGGAC ACTCTCCAAG 
AAAGAGAAGA AGAAGGAGAA GAAGCGAGAG AAGGAGGCGC ACATTGCTGA CGGAGATGAG 
CAAGCACTGA GCAGAGAAGA TGGTGAGAAC TCCAGACTGG CAGCGGAGGT GTACAAGGAC 
ATGCCAGAGA ACAGCTTCAC CAGGACCATC TCCAACCCTG AGGTGGTGAT GAAGAGGAGG 
CGGCAGCAGA AACTGGAGAA GCGGATGCAG GAGTTCCGCA GCAGCGACGG CAGGCCAGAC 
TCGGGGGGGA CGTTGAGGAT CTATGCAGAC AGTCTGAAAC CCAATATCCC CTATAAGACC 
ATCTTGCTGT CCACGACAGA CATGGCAGAT TTTGCTGTGG TGGAGGCCCT GGAGAAGTAC 
GGCCTGGAGA AGGAGAACCC CCGGGAGTAC TGCATCGCCA GGCACACAGA TGACAAGTCT 
GGGAAGGAGA TCATCCTGGA CGATACAGAG TGTCCTCTTC AGATCTTCAG AGACTGGCCC 
AACGACAGAG GGGACCTAGT GTTCCAGCTG AAGAAGAGAC CTCCAGACTA CGTGCCAAAG 
AAGGGCCGCA AGCCAGAGGA CAAGGGCCCC AGGGGGAAGG GCATGGACGT GCCCCTGCAT 
GGGTCTCTAC CCCCAGAGAA ACTGCCTTAC CTGGTGGAGC TCAGCCCAGG GAGGGGGAAT 
CACTATGGCT ACTACACCTA TCGGCACCAC GAAGACGGCT CAGACTCCAG GGACAAACCG 
AAGCTCTACC GTCTGCAGCA CAGTGTGACT GAGGTGGGCT CAGACACGAC AGATGACGGC 
TCCATCCAGC TGTTTGGTCC AGGTATCCTG CCCCACCACT GTAAACTGAT GCACACGGAG 
GGTTTGGTGA CAGTGACACC ACACAGTCTG GAGGCTGAGA CCTACGTGGA CGGCCAGCGT 
GTCGCCGAGA CCACCGTGCT GCGAAGCGGA GCCACACTGC AGTTCGGAGC CTCCCATGTC 
TTCAAGTTTG TGGACCCCAG CTACGACCAC AGCATGGGCA AGGGAGGGCC CGGACCAATG 
ATGAGAGGAA GGCACAAGTC AGGAAGTGTT CCTGAGGCGA CCTTTGACCT GCAAGGGGAC 
CTCCACAGTG GGACTGCCCT CTCCACCAGC AAGAGCTCAG GGAAGCTGGA AATGGAGCGT 
TCATCCAACG GCAGCGAGAG GGGCATGGTC AAACCCATGA TCCGCGGGGA GCAGCAGGAC 
AGCAGGTCCC AGGACAGCCT GAGCCCAGAA GTGACGCTGC CGGCCAGCAT CGAGTTCAGG 
GACAACTCTG AAGATACCTT CCTGACGGCC ATCATCAACT ACACCAACAG CTCCACGGTG 
CACTTCAAGC TGTCCCCCAC CTACGTACTG TACATGACCT GTCGCTACGT CATGTCCAGC 
CACTACAGGC CCGACATCGG TCCCTCGGAG CGCACCCACA AGGTCATAGC CATCGTCAAC 
AAGATGGTGA GCATGATGGA AGGAGTCATC CAGAAGCAGA AGAACATTGC GGGGTCCCTG 
GCTTTCTGGA TGGCCAATGC CTCAGAGCTG CTCAACTTCA TCAAGCAGGA CAAGGACCTG 
TCGCGCATCA CACTGGACGC CCAGGACGTG CTGGCACACC TCGTCCAGAT GGCCTTCAAG 
TACCTGGTCC AGTGTCTGCA GTCTGACCTG AGTAACTACA TGCCTGCCTT CCTGGACGAC 
CCAGAGGAAC AGAACCCTCA AAGGCCCAAA ATAGAGGATG TTTTGCACAC CCTGACGGGC 
GCCATGTCGC TGCTGCGTCG TTGCCGTGTC AACGCGGCGC TGACCATCCA GCTGTTCTCC 
CAGCTCTTTC ACTTCATCAA CATGTGGCTG TTCAACAAGC TGGTGATGGA GGCTGACTCT 
GGACTGTGTT CACACTACTG GGGCGCCATC CTCCGCCAGC AGCTCAGCCA CATCGAGGCC 
TGGGCAGAGA AACAGGGCCT GGAGCTGGCC GCCGACTGCC ACCTCAGTCG CATCGTACAG 
GCTACGACTC TCCTCACCAT GGACAAGTAC TCCATGCAGG ACGTGCAGAA CATCCACAAC 
ACCTGCTTCA AGCTGAACTC CCTACAGCTC AACGCCCTCA TGACCAACTA CCACTGTGCT 
CCCGACGAGC CTTACATCCC CCCAGAGCTC ATAGACCACG TGGTTGCCGT GGCGGAGAAC 
ACAGCAGATG AGCTGGCCCG TAGCGACGGC CGAGAGGTCC AGCTAGAGGA GGACCCTGAC 
CTGCAGCTGC CCTTCCTACT GCCCGAGGAC GGCTACTCCT GCGACGTCGT GAGGAACATT 
CCCAACGGAT TCCAGGAGTT CCTGGACCCA CTTTGTCAGA GAGGATTCTG TCGGCTAACC 
CCTCACCCGC GCTCCCCTGG GACCTGGACC ATCCACTTTG AAGGAGTCGA CTGCGACAGC 
CACTTCTCTG CAGACAACTC AGACCTGCAA ATGCGGAAGG AACCGGAAGT TGTGACGGTC 
ACCCTGAAGA AGCACAATGG CATGGGCCTC AGCATTGTGG CTGCCAAGGG TGCTGGGCAG 
GAGAAGCTCG GGATCTACAT CAAGTCTGTT GTGAAGGGAG GCGCGGCTGA CGTGGACGGG 
CGTCTGGCTG CAGGGGACCA GCTGCTGAGT GTGGACGGCC GCAGCCTGGT GGGCCTCTCC 
CAGGAGAGGG CTGCGGAGTT GATGACGAGG ACAGGTTCTG TGGTAACACT GGAGGTGGCT 
AAACAGGGAG CGATCTACCA CGGCCTGGCC ACGCTCCTCA ACCAGCCATC TCCTATGATG 
CCCAGAGCGT CGCAAGGAGG GCGTGACAAG AATGGCAAAC TGAGACCAAA GAGCGAAGGC 
TTTGAACTGT ACAACAACTC GGTCCAGAAC GGCTCTCCAG AGAGCCCCCA GGCCGACTGG 
CAGGCCTACC CAGAACCAAA GAAGATGAGC GGGGAGGAGA GGCTCCTGAA GAACCGGGCC 
GACCACCGCT CCAGCCCCAA CGTGGCCAAC CAGAGCCAGA GTCCGGGAGG GAAGGCCGTC 
TACACACGAG GACCTGGGAC CAAGATCACC TCTGTCTCCA CGGGCAACCT GTGTGTTGAT 
GACGAGCCCT CTCCTCCCCA TCCGGAGGCC TATCCTATTC CCACTCAGAC CTACCCCAGG 
GAATACTTCA CCATCCCAGC CTCCAAGTCT ACGAACCGGA TGGACCCGGC CCCAGGACAG 
TTATACGCTG ACGACAGGGG TCCTCCCGGC CCCATGGAAG ACCACCACCA TAACAGCATA 
GCTATGCAGC GGGTGGACCA CTCTCAGGAG GAGCTGTGTG ACCTCCCGGG GGGCTACCCT 
CCAACCAGCC TGCGTCCTGA GGACCTGGTC CACTTGCACC AGCAGCAGCA GAGAGAGGCT 
GACTACCAGT GGGGCAGTGG GGAACACTGG GTCAACCACC ACCAGGTCTC CTCTTCCGTG 
GAGTCCTCCA CCTCCAGCCA GGAGCACCTC AACTACTCCT CGTCCTCCTC CAACAAGACC 
CAGAGCAACC ACAAGAGTGG CCCCGGACGC TGGAAGACCC CCGGCCCCAT TGCCCCCCAC 
CCAGGCCCCG TGGCCCAGTC CACACGCACG GACCTCCTTC CCCCTCCACC GCCCCCTCCC 
CCCGTACACT ACCAGGACTT TGAGCTCCAG CCCGACCTGC CCCTGCCCCC TCCTCCTTCG 
GTCACCCAGC AGCAGGCCGC AGCAGCAGCC GCCGACCGCA AGAAGCGCGA GGAGCAGCAG 
CGCTGGTATG AGAAGGAGAA GGCCCGGTTA GAGGAGGAGA GGGAGAGGAA GAGGAGGGAG 
CAGGAGAGGA AGCTGGGACA GGTCCGTAAC CCAGCTGTGC CTGTAACCCA CAACAACAAC 
CAACACGGGG GCACACTCCC CCCTCCCACA CAGCACCAGC AGGTGCCCCC TCCCCGGTCC 
CCCATGCAGC AGGTGCCCCC TCCCCGGTCC CCCATGCAGC AAGTGCCCCA TCCCTACCTC 
CCCCCCCAGG GCCAACAGCA GGCCCAGCAG CAGCAGCCCC CCAGGCCAGA GAAACTGTCC 
ACCCTGCCCA GAGCGGGCAC CCTGCCCCCT CAGGGTAACC CCATGGACAC AGTGATCAGA 
GAGCTGCTCC CCCAGCAGCA GCCCCGCACC ATCGAGCGCC GGGACCTGCA GTACATCACC 
ATCAGCAAGG AGGAGCTGTC CTCCAGCGAC AGCCTGTCCC CAGACCCCTG GAAGAGGGAT 
GCCCGGGAGA AGTTGGAGAA GCAGCAGCAG CTGCACATCG TAGACCTGCT GGACAGGGAG 
ATCCAGGACC TGCAGGCCAA GCCGGAGCGT ACGGCCGAGG AGAGCGACCG GCTCAGGAAG 
TTGATGTTGG AGTGGCAGTT CCAGAAGAGA CTGCAGGAGT CCAAGCACAG TGATGAGGAA 
GATGAGGAGG ATGACGACGA GGACGTGGAC ACCATGATGA TCATGCAGCG GCTGGAGGCA 
GAGAAGAGAG CAAGGCAGAC TGCTGTCCCA GCAATCTCTG TTCTAGACTT GTTGCAGGAC 
GAGGAGCGGC GGAGGAAGCA ACAGCTGGAG GAGATCCGTA AGCGCGAAGC GGAGGACCGG 
GCCAAACAGG AGGAGGAGAG ACGCTGGAGA GAGGAGGAGA GGGCCAGACG AGAGGTCGAT 
GAGAAGAGGA GACAGGAGGA GGAATACTAC ACACGCCTGG AGGAAGGGCG GCGCCGGCAG 
CACGAGGAGG CCGAGCGCAA GCTGCTGACG CCTGACGAGC CTGGTCTGTA CAGACCTCCC 
CTCCCCAGGG ACTACCAGCC TCAGCCCCCC CCTCCCACTG CATCCCCCGC AACCACTGCC 
ACTAACGCTC CACCACCCCC GCCTCAGAGA AACACTTCCT ACCTCAAAAC CCAGGTCGTC 
TCCCCTGACG CCTTGTACAC TGCCAAGTTT GTCTCGTACA ATGACGAGGA GGATGAGGAC 
ACTACAGGCC TAGCAGGTCA GGTTAAGCTC TCCTCCACCA GGAAGTCCTA TGGCGACCTC 
CCGCCCGCGC ACAAGCCGCA GCCGCCTGCG CGCAAGCCCC GTCCTATGTC TGATGGGATC 
TTTCTGTCCA GCTCATTCCA GCCGCCGGCT GTCAATGCCA ACAGTACTGC AGGGCAGCCC 
CCTCCCCCTC CCATCAAACC CAGCCTCATC CCGACAGGCA ACTTTAAAGG ATTTAATTCA 
TACACTGGAA ACACAACAGG GGTTGTAGTA TCAGGAGAAG TTTACAAGGA CCTGCGAGAA 
AAGTGGACGA AAGGTGGTCA AGAACAGGAA AACACTTTAA CTGAAGGAGC CCCGGAGAGT 
ATGACATTCA AGGAGCGCCA GCGGCTCTTT TCCCAAGGGA AGGAAGTATC CAACAAAGTC 
AAGGCCTCGC GAAAACTCAT GGAGCTGGAA AATGAACTCA ATACAAAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gene Symbol	LOC106571391
Entrez Gene ID	106571391
Full Name	afadin-like
Gene Type	protein-coding
Organism	Salmo salar(Atlantic salmon)

LOC106571391 cDNA ORF clone, Salmo salar(Atlantic salmon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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