spg11 cDNA ORF clone, Salmo salar(Atlantic salmon)

Gene
Clones
gRNAs

Summary

Gene Symbol	spg11
Entrez Gene ID	106560754
Full Name	SPG11, spatacsin vesicle trafficking associated
Gene Type	protein-coding
Organism	Salmo salar(Atlantic salmon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014123970.1	XP_013979445.1	spatacsin isoform X1
XM_014123971.1	XP_013979446.1	spatacsin isoform X2
XM_014123972.1	XP_013979447.1	spatacsin isoform X3

Pathways from BioSystems
Gene Ontology

The following spg11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spg11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAt32026	XM_014123970.1 Latest version!	Salmo salar spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAt32027	XM_014123971.1 Latest version!	Salmo salar spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAt32028	XM_014123972.1 Latest version!	Salmo salar spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spg11 ( XM_014123970.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013979445.1 Salmo salar spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X1, mRNA.

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CloneID	OAt32026
Clone ID Related Accession (Same CDS sequence)	XM_014123970.1
Accession Version	XM_014123970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012336573.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTATGA TAAATATGTT GCACAGATCT GAAACTCGAG ATAACTGTGC ATTAGAAGTC 
GCTCTCATCC CGGAGAGCAA CCTCTGTGTA AATGTTGTGG ACACAGCAAA AGCCAAAATG 
GCCCAAGGCT GCTCCTTGCT CTGCTGCTTG AGTTGTGCTG GGGGGCTGAC AGTCGGCGAC 
ATTTCAGAGA GCAAGGCACC GGGGAGGACG GTGGATGGCA CATATACAGA CTTTGCCTGG 
GAGGAGGTGA CAGTTTACAA TGGAGCCCGT GGCAGCCCTA GACTTCTGGC TGTGAGCTCT 
GAGTTTGAGC TGACACTGTA TGAACTGGAA GCAGAGGGCC CTACCTCTGG CCCTCTTGTC 
TCTGCCTCTG AGTTCACAGC AGATAAGCTA CTGCAGCTGG TCAAGGAGAA AGCCCTGAGT 
GTGACTGAGG TGGTGTCCTT GAGGCTGCTG TCCTTTGTGG ATGCCAGAAG CTTTCTCCTG 
CTCAACTCCA GTGTTGTGGT GCAGCTGCAG TGGCAGCAGG GGGCTGAACC GGAGACCCTG 
TCCTGCTGCA GCATCGTTCT GCCCCCAAAA GCCCCCCTGG ACACATGCAC TGACTACCAG 
CTCTGCAGAG GCATACTCTT TGTGTTGACC ACAGCTGGAG TCATATATGT GTTTGACAGT 
GCTGAGGGGA GGAAGCTGGC CAGTGTAGAC CTGCCCTCGT ACCACACTGC TGGGCTGGGG 
GAAGACGAGC CGGCCCCCTA CCTGGACTCC TTCTGTCTCC TCCAGGTGTC TCCTGACCTG 
GGTACTGCTG TTGCTGCCAC TCAGTCTAAC ACTGCCTTTG CTGTCGACCT CAACCACTAC 
TTCAGGATGT ACCCAGAGCA TCTGATGTGT CGGGTGGTCC CCAGTCGCCC TCCCCTCAGG 
CCCCAGGGTC CCTGTGACGA GGACAGCCTC TCCATCTCCA CCTACAGCCA CACTGTGCTG 
GGCACCTCCT TCAGCACTGA CCGGTCCTGG GAAGCTCGGC TGACTTCTCT GTACAACAAG 
GCCAAGGCTA CCCCTCCCTC TTCTCCTCAG ACTCCGGGGG CTCCTTGGTT TAAGGATTTC 
CCCCACATCG AGTGTGGGCG GGCAGCTATC GCTGCCAAGC TCTCCCAGAG CAGAGTCCCA 
TCAGGGGGAG CCACCATGCA GTTCTCTGTG CCCGACACAA CTGCCCCAGT GCTGCTCTCT 
GTCTCTGAAT TCTCCACTGT GTTGACCTTT GTCTCCCCTG GCAACAGCGA GACAACTGTG 
GCCTTCTGGG ATCTGCAGTC CCAGAGTGTT ACCTACCACC GGCCCACCTT GCCCACCGTG 
CCTATCCAGC GTACCGCAGA GGAGCAGCTC AGCCTGCTTC TGAAGACGTC AGGCCTGTCC 
CTGGTGCTGT TTGCTGTGTC ACAGGAGGAG CTGCTCAACA GACTAATGGT ATTTGGCAGT 
GCTGGCACAG TGGACTCTCT GTGCCACCTT AATGAATGGG GCCGCTGCTC CATCCCCATT 
CACGCCCTGC AGGCTGGTCT AAAGAACCAC CAGTTGGACA CAGTGGACTT CTTCCTGAAG 
AGCAAAAAGA ACATCCTGAC TCCACCCTCT GGGTATATTC TACCTGACCA ATCAGCTGCC 
ATCTCAACCC AGAGCCAGCT GAAGAATGTC CAGGATCTGT GTCCGGCTCT GGACCTTCTG 
CGTTGTGCCA TCAGAGACAA TCATACTGAG GCCCAGAGCC GACAATTTTC AGAACAGCTG 
CTCAGCATCA CCCTGGCCTT CCTCAACTCA CAAGTCAGGG CCGTCCTCTC TGCCACAGAG 
GAGCTGGATG AGAACTTGCA GGAGTGTGTG GAGATCTTGG ATAGTTACGT CTCGGATCTG 
CGCACCTTCA TGAAGAGGTT TCCCTGGCCA ACAGGTGGTG ACACTTCCAC CCTTGTCCCA 
TCCCCCATAC CTGTACCTGA GGAGGTGGAG AATGATGACT GGAGTTCCCT CTCTTCTGAG 
GAGGTGGTCG AGCGGGCAGT GCTGACCAAG CAGATTCCCC GGGCCCAGGC CTACCTGAGG 
AGCCAGAACA GGCCAGATCA GCGGCTGGAG GAGTTGAGGA GTACGGGGCT GCGTCTGGCC 
TTCAACTGCC TCACACACAG AGACCTGGAG CAGGCCACTG CACTGCTCAG GAACATGGGT 
TTCAACGTAA AGAAGCAACT GTATAACATA TGCCTCTACA CAGATGACCG GGACCTAAGG 
GACTTTGTGG TGGAGGAGTT GTCAAAGCAG AGCTATTTGT CTGATGAAGA GACACAGAGG 
GTGGAATTCA TCAAACAGAT TGAGGCTTTG TGTGCGGTGC CTGAGACGCG CTGCACAGAC 
CCTGTGGGCA GACAACGGGT TCCTCAGATG GCCCAGACAG ACCCAGAGTC TAAGAGCCTG 
CTGGAGGAGC TGGTAGACCA GAGAGGCTCC CAGTCAGAAC AGACACTGTG GAGTTCGCTG 
CGTCTGGACT GGGTCAGACA TTGGGACCAC AACACCCAGA GAGCCATCCT GCTGTCACGC 
CTACAAGACT CGGCAGTTGT CAGTTCCTGT GACCCGGGGG TCCTGTGGTC ATACCTCACC 
TCCCACCACG ACCAGCGGAG GGTAACCGAG TGGATTGAGA GCCTGGCCGC CCAGAACGGT 
GACCCTTGCA GCGCCCCCCA GTGGCCCGCC CTCACGGCAG AGGTGGTCAA CAACAACACC 
CGGTGTAGCG CCCACATGAG GAACCACATC CTCTACATGT TGGCCAGGAG AGGAGTGTTC 
ATCCAGGCCG AGATGGCTGA TTTTGAGCAT CTGTTGTGGA GGCTGGGCCA GGCAGGTGGG 
GTGATGCAGG CCAGCCCACC CATGCCCCAG TACCGCTCCC CTCAAGGACA CGACTTACAC 
ACACGCTTCA TCCACCACTG CCTGGACAAC GGCCTTCGCT ACCTGCTCTA CACCTACCTG 
GAGCACAACA GATTGACCCC CAGGAATTGC CCTGTCCTTG CTGATAATGG TCTATATGAA 
AGTCACCCGT GGTTTGAAAT GCTGGTGAAA ATGCAAGAGA TTACCAGAGA CCTCTCAGAT 
CCAGAACGCG TGTTCCAGGC CAGTCTGACC AGTGCCCAGG TCCTCATCCC AGGCAGCCAG 
GCCATGGTTA GCAGCATGCT CCTGGAAGGA CACAGTCTTC TGGCCCTCGC TACCATCATG 
TTTGCCCCGG GTGGCATCGA CCAGGTGGTG AGTCAGGGGG AGAGGGCTAG GAGCTCTGTG 
TCTAGGGTGG ACCCCCAGCT ACTGAAGATG GCCCTGAGCC CCTACCCCAA GCTCAAGGCA 
GCGCTCTTCC CTGTGAGCGG ACCACGAGGG ACCACCGCCA CCTCATCCGA TATCTCCGTC 
TACCACCTCA TGCAGTCTTT ACATCCATTG GATCCATCTC GACTGTTCGG GTGGCAGTCT 
GCCAATACAC TAGGCTCCAC CGAGACCTCA GTGGAGCTGC CCCATTTCTC TAGTCCTCAC 
CTCGTCAGTA AATACGCCCT CATTGAGAAC CTCGACTTCC TGTACTATCT GCGTCACGGA 
CGACCATCGT TTGCCTACGG AACGTTTCTC GTCCAACAGC TAGCCAACTC CAACGAAGTG 
AAGTCGCAGC TCAGTCATGC GGCGGAGCAG GCCTACAGCC TGGCCATGCT GTATTTCAGC 
GTTCCCTCTG TGGCAGCTGC ATGTGTGTGT TTCTGCGAGC TCCTGGGTGT ATGTAGCCTA 
AAGTTGCGGG TGGACCTCAA AGCCCTCAGT CTGATCCTCC GACTCCGGAG CCAGAGGCCT 
GGAGGGACCA GCACATTCTC CCTTAAAGAC TTACTGGCTC CTAAAGGCAG TAAGCTGGTG 
GAGGTGGAGA GGGAGACAGC GGAGGAGCTG CTCTTTGACT TGGAGGCAGC TGTGAAGGAC 
AGTTTGGAGA GGAAGGGCAT CAGCAGGTCA TCCTATGAGG CAGGTCAGGA GTGGGCTCTG 
CCCATACAGT TTTGCCAGCT ACATGCCCTG CCCCTGAGCA GCGTGTACCC ACACGACTGT 
GCAGCCGACA GCCAGTGGCT ACACTTCCTG CTCTTTGTCC AGCTGCACAG CTACCCCCCT 
CAGCAGGTGA GGTCCCTGGC GACCCATTTC AGCCCAGCCC TGCAGGCCCA CCTGACCCTG 
GCCTTCCAGA ACCTGCAGCT CAGCTCTCAG TGGAGGGTGG GGGAGGTGGA AGACAGGGAG 
GCCCAGGGTC AAGTCACCCT GCCCAGCGAG GAAGCCTCCG ACCCCCCCAA GGAGCTGTTC 
CAGGTGCTGC TGCAGAGCCA GGAGCAGGCC AGCCCCTGGA GACACCTCCT GGGGGAGGCC 
CTGGCACAGC ACTGCCCCAC GCTCACCACA CTAGCTGCCT GTCATCAGGA AGCCGACCTG 
CTCCAGTGCC TCTGCGTCTG GGTCCTGACC TCTGTGGATG AGGTCATTGC CACAGAGGCT 
ACCGCCCACC TGGAGGAGAG CCCGCGACGT CATGAGTGGA ACCTCCACGA TCTGTCAATC 
ATCTGGAAGA CACTGCTGAG GAAATGCAGG GTCAGGCCTC TCATACGAGG CTTCCAGCTC 
TTCCAGAGGG ACTCTCCCCT GATCCATGTG CTGCTCATGT ACGAGCTGTG CTACGACTAC 
AAAAACTACC CTGAAGCCAA GAAGAAACTC ATTGAGTTCC AGAAATGCCT GATCACGCTG 
AGGAGCAGTG GTGCTCATGC GCCAGCGGGC ATCCCTCTCC AGTGGGTGGA GTGCCAGGCC 
TCAGTCCTCC TGTTGACCAT GATGCAACAG TGCTCCACAC AATACGAGCT GCGCAGACTA 
CTGCAGCTGC TCGCAGACAT GGAGCGCCTG CTCAAGTCTA ACGGCCCGGA CTTCAAGAAG 
CTCAGCTTCC TCAGCCAGGT TCTCCAGGAC ACCTCGGTGT CTCTCTCTCC CAGGCTGCTT 
GAGAGCTATT CCCCTGAAAT CCTGCAGGGA GAGTGCCAGA GCATGTTGGA GAAGCTGCAA 
GACAGCGGCC TGTTCCACAA GGCCCGCCAG GTAGCTGAGC TGGCAGGGCT GCCCATTGAC 
AGCCTGGTCA TCAACGAGTT ACTCCAAGAC CTCAGTGCCC TGAAGGCCAA GCGGCACTGG 
GAGAGGAGGG AGACCCGGGT GTCCTTCTGG AGGAAATGCC ACGAGCAGTT CAGGACTAAC 
AGCACTGGCC CTGGGGCCGC CTCAGAGTTC TTCCTGGCCC AGGCAGGTGC CTCTGAGCCT 
CACCGGGGCT CCGTGGCCGA GACAGAGCTG CTGTGTGTCC AGGAGAGGTG CCTTCTCCTC 
AGCCTGGCCG GCCACTGGCT GTCCTGGCTC ACCCTAGCTC CCCTGGCACG TCTCGAGGAC 
CTGGAGAAGC AGCTGTGGAC AAGCCGAGTG CGCCAGCAAG TCCTGCTCAC CGCCATGGAG 
AAGGAGAGCA TGTTCGCCCT CCCGGCGCCC ACCATAGCCC CCGAGGAAAA CTCCTATGAG 
GCCCTGATCA GGGAGTTCTC CTTCTCCAAG ATGGCAGTGC TCAACGAGTC CAGGTACCTG 
AGCTTAGACG GCCTGCCCAG CTTCTCCCCT GAGGAAGAGG GTGGGGAGAA GACTGGGTCG 
GAGGAGCAGG GTGCTCTGGC TATCCTGGTG GGGCAGCTGT TGGACGAAGG GTGTATCCAC 
GAAGCGAGCC GTGTGTGCCG TTACTTCTCC TTCTGCTCCC GGGACATGTG GGTAGTGCTG 
CACTGCCGAG GCCTGGCCTC AGGCGAACTC CAGGCTGGCC TGCAGGACAT GCTCACCTTT 
GAGGGCCTGC CCAAGAAGAG CATTCCCAGC TCAACTAGTT TTAGCAGCCT GTCCTCCTTT 
GTGATGGTGC CCTCTCCAGA GGATCAAACG GTGACTCAGC TCCAGATACT GGTGGACGAA 
TGTCGCCACG GAAAGAGCTA CTGCAAGCAG GTCCTGAGTC TCTACGAGCT CTCCAAGGAG 
CTGCAGTGTT CATACAGTGA GATCTCAGCC GAGGAGCCTC AGGCAGTGCT GTGTAAGGTG 
CTACTGTCCC AGCAGCCGGA GCGCTATAAG AAGGCCCAGG CCTTCATCAG TGTGCAGGGC 
CTGCAGCCTG ACACCGTGGC CCAGCTTGTA TCCACTGCCG TGGTCCAGGG CCTGTTGGCC 
TCTGCACAGG ACGGAGAGAC TGCAGAGAAG CAGATGTTCA GTCCAGAGGA AGGGAGAGAG 
TCCTTCTTGC AGCTGGCCAA GCTGTGTGGG GACCCCAACC TGGTGGGCAT CAAACTGCTG 
GACCACATCC CCACTGTGCC ACTGGGAGAG CTCGGCTGCA CGGTGGAGCT GTTGATCCTG 
GCCCACGATT GCTTCAGTCT GACCTGTAAC ATGGAGGGCA TAGTGAGGGT GCTGCAGGCT 
GCCCGCCACC TTAGCCACGC ACACCTGGCC CACAGCGAAC ACTACGGCCT GCTGGTGCGT 
CTCCTCACTG GCATCGGCAG ATACAATGAC ATGACCTACA TCTTCGACCT GCTCAACCAG 
AACCACCGCT TCGAGATGCT GCTCAGGAAA AAAGTGGAGT CGAACTTCCG GCTGAAGACG 
GCCCTGCTGG ACTACATCAA GCGGTGTTTG CCGGGGGACA GTGAGAAGTA CAACATGGTG 
GCCCTATGCT TCAGCATGTG CAGAGAGATA GGAGAGAACC ACGAGGGAGC CGCCCGCACC 
CAGCTCAAAC TGATCGAGTC CCAGCCTTGG GCAGTGACCC CTGAACTCAA AAGTGCCTTG 
ATAAAGGTTC TTACTCTCCT GAAGGATGCT GCAGAGAGCT ATTCTAAGGA CTCGTGTGTA 
CGCCAGGCAG CACGTTGTGT CAAAATGGCA AAGCTGACCA CACTCCAGCT CCACTTCCTG 
AACCACGGTC AAGACCAACG TGTCATAAAC CTGCGCCAAT CAGATCTCCT TGGTGCTATA 
GTGGCACTGC CCCGCTGTTA CCAGGCTTTT GTGTTGTCGG AGGCCTACGA CTACAGTCCA 
GACTGGGCGG AGGTGCTCTA CCAGAAGGTC ATCCTCAGTG GAGACTTTGC TTACCTAGAG 
GAGTTCCGAC TTCACAGGCC ACTCCCCGCC TGCCTGTTTG AGGACATATC ACAAAAATTG 
ACTCACAACA CGCCTCCGAG CAGTGCGGGC CAGAACCTGA AGAGACTATT GCAACACTGT 
GATGACGTTT ACACCTACTA CAAGCTTGCG TATGAACACA AGTTCTTCGA CGTGGCCAAC 
ATGTTACTTC AGGACTCCAA AACAAGCAGT TACCTAAATG ACAGGCTAGG TACTAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013979445.1
CDS	220..7479
Translation	MIMINMLHRSETRDNCALEVALIPESNLCVNVVDTAKAKMAQGCSLLCCLSCAGGLTVGDISESKAPGRTVDGTYTDFAWEEVTVYNGARGSPRLLAVSSEFELTLYELEAEGPTSGPLVSASEFTADKLLQLVKEKALSVTEVVSLRLLSFVDARSFLLLNSSVVVQLQWQQGAEPETLSCCSIVLPPKAPLDTCTDYQLCRGILFVLTTAGVIYVFDSAEGRKLASVDLPSYHTAGLGEDEPAPYLDSFCLLQVSPDLGTAVAATQSNTAFAVDLNHYFRMYPEHLMCRVVPSRPPLRPQGPCDEDSLSISTYSHTVLGTSFSTDRSWEARLTSLYNKAKATPPSSPQTPGAPWFKDFPHIECGRAAIAAKLSQSRVPSGGATMQFSVPDTTAPVLLSVSEFSTVLTFVSPGNSETTVAFWDLQSQSVTYHRPTLPTVPIQRTAEEQLSLLLKTSGLSLVLFAVSQEELLNRLMVFGSAGTVDSLCHLNEWGRCSIPIHALQAGLKNHQLDTVDFFLKSKKNILTPPSGYILPDQSAAISTQSQLKNVQDLCPALDLLRCAIRDNHTEAQSRQFSEQLLSITLAFLNSQVRAVLSATEELDENLQECVEILDSYVSDLRTFMKRFPWPTGGDTSTLVPSPIPVPEEVENDDWSSLSSEEVVERAVLTKQIPRAQAYLRSQNRPDQRLEELRSTGLRLAFNCLTHRDLEQATALLRNMGFNVKKQLYNICLYTDDRDLRDFVVEELSKQSYLSDEETQRVEFIKQIEALCAVPETRCTDPVGRQRVPQMAQTDPESKSLLEELVDQRGSQSEQTLWSSLRLDWVRHWDHNTQRAILLSRLQDSAVVSSCDPGVLWSYLTSHHDQRRVTEWIESLAAQNGDPCSAPQWPALTAEVVNNNTRCSAHMRNHILYMLARRGVFIQAEMADFEHLLWRLGQAGGVMQASPPMPQYRSPQGHDLHTRFIHHCLDNGLRYLLYTYLEHNRLTPRNCPVLADNGLYESHPWFEMLVKMQEITRDLSDPERVFQASLTSAQVLIPGSQAMVSSMLLEGHSLLALATIMFAPGGIDQVVSQGERARSSVSRVDPQLLKMALSPYPKLKAALFPVSGPRGTTATSSDISVYHLMQSLHPLDPSRLFGWQSANTLGSTETSVELPHFSSPHLVSKYALIENLDFLYYLRHGRPSFAYGTFLVQQLANSNEVKSQLSHAAEQAYSLAMLYFSVPSVAAACVCFCELLGVCSLKLRVDLKALSLILRLRSQRPGGTSTFSLKDLLAPKGSKLVEVERETAEELLFDLEAAVKDSLERKGISRSSYEAGQEWALPIQFCQLHALPLSSVYPHDCAADSQWLHFLLFVQLHSYPPQQVRSLATHFSPALQAHLTLAFQNLQLSSQWRVGEVEDREAQGQVTLPSEEASDPPKELFQVLLQSQEQASPWRHLLGEALAQHCPTLTTLAACHQEADLLQCLCVWVLTSVDEVIATEATAHLEESPRRHEWNLHDLSIIWKTLLRKCRVRPLIRGFQLFQRDSPLIHVLLMYELCYDYKNYPEAKKKLIEFQKCLITLRSSGAHAPAGIPLQWVECQASVLLLTMMQQCSTQYELRRLLQLLADMERLLKSNGPDFKKLSFLSQVLQDTSVSLSPRLLESYSPEILQGECQSMLEKLQDSGLFHKARQVAELAGLPIDSLVINELLQDLSALKAKRHWERRETRVSFWRKCHEQFRTNSTGPGAASEFFLAQAGASEPHRGSVAETELLCVQERCLLLSLAGHWLSWLTLAPLARLEDLEKQLWTSRVRQQVLLTAMEKESMFALPAPTIAPEENSYEALIREFSFSKMAVLNESRYLSLDGLPSFSPEEEGGEKTGSEEQGALAILVGQLLDEGCIHEASRVCRYFSFCSRDMWVVLHCRGLASGELQAGLQDMLTFEGLPKKSIPSSTSFSSLSSFVMVPSPEDQTVTQLQILVDECRHGKSYCKQVLSLYELSKELQCSYSEISAEEPQAVLCKVLLSQQPERYKKAQAFISVQGLQPDTVAQLVSTAVVQGLLASAQDGETAEKQMFSPEEGRESFLQLAKLCGDPNLVGIKLLDHIPTVPLGELGCTVELLILAHDCFSLTCNMEGIVRVLQAARHLSHAHLAHSEHYGLLVRLLTGIGRYNDMTYIFDLLNQNHRFEMLLRKKVESNFRLKTALLDYIKRCLPGDSEKYNMVALCFSMCREIGENHEGAARTQLKLIESQPWAVTPELKSALIKVLTLLKDAAESYSKDSCVRQAARCVKMAKLTTLQLHFLNHGQDQRVINLRQSDLLGAIVALPRCYQAFVLSEAYDYSPDWAEVLYQKVILSGDFAYLEEFRLHRPLPACLFEDISQKLTHNTPPSSAGQNLKRLLQHCDDVYTYYKLAYEHKFFDVANMLLQDSKTSSYLNDRLGTR

Target ORF information:

RefSeq Version	XM_014123970.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014123970.1

ORF Insert Sequence:

ATGATTATGA TAAATATGTT GCACAGATCT GAAACTCGAG ATAACTGTGC ATTAGAAGTC 
GCTCTCATCC CGGAGAGCAA CCTCTGTGTA AATGTTGTGG ACACAGCAAA AGCCAAAATG 
GCCCAAGGCT GCTCCTTGCT CTGCTGCTTG AGTTGTGCTG GGGGGCTGAC AGTCGGCGAC 
ATTTCAGAGA GCAAGGCACC GGGGAGGACG GTGGATGGCA CATATACAGA CTTTGCCTGG 
GAGGAGGTGA CAGTTTACAA TGGAGCCCGT GGCAGCCCTA GACTTCTGGC TGTGAGCTCT 
GAGTTTGAGC TGACACTGTA TGAACTGGAA GCAGAGGGCC CTACCTCTGG CCCTCTTGTC 
TCTGCCTCTG AGTTCACAGC AGATAAGCTA CTGCAGCTGG TCAAGGAGAA AGCCCTGAGT 
GTGACTGAGG TGGTGTCCTT GAGGCTGCTG TCCTTTGTGG ATGCCAGAAG CTTTCTCCTG 
CTCAACTCCA GTGTTGTGGT GCAGCTGCAG TGGCAGCAGG GGGCTGAACC GGAGACCCTG 
TCCTGCTGCA GCATCGTTCT GCCCCCAAAA GCCCCCCTGG ACACATGCAC TGACTACCAG 
CTCTGCAGAG GCATACTCTT TGTGTTGACC ACAGCTGGAG TCATATATGT GTTTGACAGT 
GCTGAGGGGA GGAAGCTGGC CAGTGTAGAC CTGCCCTCGT ACCACACTGC TGGGCTGGGG 
GAAGACGAGC CGGCCCCCTA CCTGGACTCC TTCTGTCTCC TCCAGGTGTC TCCTGACCTG 
GGTACTGCTG TTGCTGCCAC TCAGTCTAAC ACTGCCTTTG CTGTCGACCT CAACCACTAC 
TTCAGGATGT ACCCAGAGCA TCTGATGTGT CGGGTGGTCC CCAGTCGCCC TCCCCTCAGG 
CCCCAGGGTC CCTGTGACGA GGACAGCCTC TCCATCTCCA CCTACAGCCA CACTGTGCTG 
GGCACCTCCT TCAGCACTGA CCGGTCCTGG GAAGCTCGGC TGACTTCTCT GTACAACAAG 
GCCAAGGCTA CCCCTCCCTC TTCTCCTCAG ACTCCGGGGG CTCCTTGGTT TAAGGATTTC 
CCCCACATCG AGTGTGGGCG GGCAGCTATC GCTGCCAAGC TCTCCCAGAG CAGAGTCCCA 
TCAGGGGGAG CCACCATGCA GTTCTCTGTG CCCGACACAA CTGCCCCAGT GCTGCTCTCT 
GTCTCTGAAT TCTCCACTGT GTTGACCTTT GTCTCCCCTG GCAACAGCGA GACAACTGTG 
GCCTTCTGGG ATCTGCAGTC CCAGAGTGTT ACCTACCACC GGCCCACCTT GCCCACCGTG 
CCTATCCAGC GTACCGCAGA GGAGCAGCTC AGCCTGCTTC TGAAGACGTC AGGCCTGTCC 
CTGGTGCTGT TTGCTGTGTC ACAGGAGGAG CTGCTCAACA GACTAATGGT ATTTGGCAGT 
GCTGGCACAG TGGACTCTCT GTGCCACCTT AATGAATGGG GCCGCTGCTC CATCCCCATT 
CACGCCCTGC AGGCTGGTCT AAAGAACCAC CAGTTGGACA CAGTGGACTT CTTCCTGAAG 
AGCAAAAAGA ACATCCTGAC TCCACCCTCT GGGTATATTC TACCTGACCA ATCAGCTGCC 
ATCTCAACCC AGAGCCAGCT GAAGAATGTC CAGGATCTGT GTCCGGCTCT GGACCTTCTG 
CGTTGTGCCA TCAGAGACAA TCATACTGAG GCCCAGAGCC GACAATTTTC AGAACAGCTG 
CTCAGCATCA CCCTGGCCTT CCTCAACTCA CAAGTCAGGG CCGTCCTCTC TGCCACAGAG 
GAGCTGGATG AGAACTTGCA GGAGTGTGTG GAGATCTTGG ATAGTTACGT CTCGGATCTG 
CGCACCTTCA TGAAGAGGTT TCCCTGGCCA ACAGGTGGTG ACACTTCCAC CCTTGTCCCA 
TCCCCCATAC CTGTACCTGA GGAGGTGGAG AATGATGACT GGAGTTCCCT CTCTTCTGAG 
GAGGTGGTCG AGCGGGCAGT GCTGACCAAG CAGATTCCCC GGGCCCAGGC CTACCTGAGG 
AGCCAGAACA GGCCAGATCA GCGGCTGGAG GAGTTGAGGA GTACGGGGCT GCGTCTGGCC 
TTCAACTGCC TCACACACAG AGACCTGGAG CAGGCCACTG CACTGCTCAG GAACATGGGT 
TTCAACGTAA AGAAGCAACT GTATAACATA TGCCTCTACA CAGATGACCG GGACCTAAGG 
GACTTTGTGG TGGAGGAGTT GTCAAAGCAG AGCTATTTGT CTGATGAAGA GACACAGAGG 
GTGGAATTCA TCAAACAGAT TGAGGCTTTG TGTGCGGTGC CTGAGACGCG CTGCACAGAC 
CCTGTGGGCA GACAACGGGT TCCTCAGATG GCCCAGACAG ACCCAGAGTC TAAGAGCCTG 
CTGGAGGAGC TGGTAGACCA GAGAGGCTCC CAGTCAGAAC AGACACTGTG GAGTTCGCTG 
CGTCTGGACT GGGTCAGACA TTGGGACCAC AACACCCAGA GAGCCATCCT GCTGTCACGC 
CTACAAGACT CGGCAGTTGT CAGTTCCTGT GACCCGGGGG TCCTGTGGTC ATACCTCACC 
TCCCACCACG ACCAGCGGAG GGTAACCGAG TGGATTGAGA GCCTGGCCGC CCAGAACGGT 
GACCCTTGCA GCGCCCCCCA GTGGCCCGCC CTCACGGCAG AGGTGGTCAA CAACAACACC 
CGGTGTAGCG CCCACATGAG GAACCACATC CTCTACATGT TGGCCAGGAG AGGAGTGTTC 
ATCCAGGCCG AGATGGCTGA TTTTGAGCAT CTGTTGTGGA GGCTGGGCCA GGCAGGTGGG 
GTGATGCAGG CCAGCCCACC CATGCCCCAG TACCGCTCCC CTCAAGGACA CGACTTACAC 
ACACGCTTCA TCCACCACTG CCTGGACAAC GGCCTTCGCT ACCTGCTCTA CACCTACCTG 
GAGCACAACA GATTGACCCC CAGGAATTGC CCTGTCCTTG CTGATAATGG TCTATATGAA 
AGTCACCCGT GGTTTGAAAT GCTGGTGAAA ATGCAAGAGA TTACCAGAGA CCTCTCAGAT 
CCAGAACGCG TGTTCCAGGC CAGTCTGACC AGTGCCCAGG TCCTCATCCC AGGCAGCCAG 
GCCATGGTTA GCAGCATGCT CCTGGAAGGA CACAGTCTTC TGGCCCTCGC TACCATCATG 
TTTGCCCCGG GTGGCATCGA CCAGGTGGTG AGTCAGGGGG AGAGGGCTAG GAGCTCTGTG 
TCTAGGGTGG ACCCCCAGCT ACTGAAGATG GCCCTGAGCC CCTACCCCAA GCTCAAGGCA 
GCGCTCTTCC CTGTGAGCGG ACCACGAGGG ACCACCGCCA CCTCATCCGA TATCTCCGTC 
TACCACCTCA TGCAGTCTTT ACATCCATTG GATCCATCTC GACTGTTCGG GTGGCAGTCT 
GCCAATACAC TAGGCTCCAC CGAGACCTCA GTGGAGCTGC CCCATTTCTC TAGTCCTCAC 
CTCGTCAGTA AATACGCCCT CATTGAGAAC CTCGACTTCC TGTACTATCT GCGTCACGGA 
CGACCATCGT TTGCCTACGG AACGTTTCTC GTCCAACAGC TAGCCAACTC CAACGAAGTG 
AAGTCGCAGC TCAGTCATGC GGCGGAGCAG GCCTACAGCC TGGCCATGCT GTATTTCAGC 
GTTCCCTCTG TGGCAGCTGC ATGTGTGTGT TTCTGCGAGC TCCTGGGTGT ATGTAGCCTA 
AAGTTGCGGG TGGACCTCAA AGCCCTCAGT CTGATCCTCC GACTCCGGAG CCAGAGGCCT 
GGAGGGACCA GCACATTCTC CCTTAAAGAC TTACTGGCTC CTAAAGGCAG TAAGCTGGTG 
GAGGTGGAGA GGGAGACAGC GGAGGAGCTG CTCTTTGACT TGGAGGCAGC TGTGAAGGAC 
AGTTTGGAGA GGAAGGGCAT CAGCAGGTCA TCCTATGAGG CAGGTCAGGA GTGGGCTCTG 
CCCATACAGT TTTGCCAGCT ACATGCCCTG CCCCTGAGCA GCGTGTACCC ACACGACTGT 
GCAGCCGACA GCCAGTGGCT ACACTTCCTG CTCTTTGTCC AGCTGCACAG CTACCCCCCT 
CAGCAGGTGA GGTCCCTGGC GACCCATTTC AGCCCAGCCC TGCAGGCCCA CCTGACCCTG 
GCCTTCCAGA ACCTGCAGCT CAGCTCTCAG TGGAGGGTGG GGGAGGTGGA AGACAGGGAG 
GCCCAGGGTC AAGTCACCCT GCCCAGCGAG GAAGCCTCCG ACCCCCCCAA GGAGCTGTTC 
CAGGTGCTGC TGCAGAGCCA GGAGCAGGCC AGCCCCTGGA GACACCTCCT GGGGGAGGCC 
CTGGCACAGC ACTGCCCCAC GCTCACCACA CTAGCTGCCT GTCATCAGGA AGCCGACCTG 
CTCCAGTGCC TCTGCGTCTG GGTCCTGACC TCTGTGGATG AGGTCATTGC CACAGAGGCT 
ACCGCCCACC TGGAGGAGAG CCCGCGACGT CATGAGTGGA ACCTCCACGA TCTGTCAATC 
ATCTGGAAGA CACTGCTGAG GAAATGCAGG GTCAGGCCTC TCATACGAGG CTTCCAGCTC 
TTCCAGAGGG ACTCTCCCCT GATCCATGTG CTGCTCATGT ACGAGCTGTG CTACGACTAC 
AAAAACTACC CTGAAGCCAA GAAGAAACTC ATTGAGTTCC AGAAATGCCT GATCACGCTG 
AGGAGCAGTG GTGCTCATGC GCCAGCGGGC ATCCCTCTCC AGTGGGTGGA GTGCCAGGCC 
TCAGTCCTCC TGTTGACCAT GATGCAACAG TGCTCCACAC AATACGAGCT GCGCAGACTA 
CTGCAGCTGC TCGCAGACAT GGAGCGCCTG CTCAAGTCTA ACGGCCCGGA CTTCAAGAAG 
CTCAGCTTCC TCAGCCAGGT TCTCCAGGAC ACCTCGGTGT CTCTCTCTCC CAGGCTGCTT 
GAGAGCTATT CCCCTGAAAT CCTGCAGGGA GAGTGCCAGA GCATGTTGGA GAAGCTGCAA 
GACAGCGGCC TGTTCCACAA GGCCCGCCAG GTAGCTGAGC TGGCAGGGCT GCCCATTGAC 
AGCCTGGTCA TCAACGAGTT ACTCCAAGAC CTCAGTGCCC TGAAGGCCAA GCGGCACTGG 
GAGAGGAGGG AGACCCGGGT GTCCTTCTGG AGGAAATGCC ACGAGCAGTT CAGGACTAAC 
AGCACTGGCC CTGGGGCCGC CTCAGAGTTC TTCCTGGCCC AGGCAGGTGC CTCTGAGCCT 
CACCGGGGCT CCGTGGCCGA GACAGAGCTG CTGTGTGTCC AGGAGAGGTG CCTTCTCCTC 
AGCCTGGCCG GCCACTGGCT GTCCTGGCTC ACCCTAGCTC CCCTGGCACG TCTCGAGGAC 
CTGGAGAAGC AGCTGTGGAC AAGCCGAGTG CGCCAGCAAG TCCTGCTCAC CGCCATGGAG 
AAGGAGAGCA TGTTCGCCCT CCCGGCGCCC ACCATAGCCC CCGAGGAAAA CTCCTATGAG 
GCCCTGATCA GGGAGTTCTC CTTCTCCAAG ATGGCAGTGC TCAACGAGTC CAGGTACCTG 
AGCTTAGACG GCCTGCCCAG CTTCTCCCCT GAGGAAGAGG GTGGGGAGAA GACTGGGTCG 
GAGGAGCAGG GTGCTCTGGC TATCCTGGTG GGGCAGCTGT TGGACGAAGG GTGTATCCAC 
GAAGCGAGCC GTGTGTGCCG TTACTTCTCC TTCTGCTCCC GGGACATGTG GGTAGTGCTG 
CACTGCCGAG GCCTGGCCTC AGGCGAACTC CAGGCTGGCC TGCAGGACAT GCTCACCTTT 
GAGGGCCTGC CCAAGAAGAG CATTCCCAGC TCAACTAGTT TTAGCAGCCT GTCCTCCTTT 
GTGATGGTGC CCTCTCCAGA GGATCAAACG GTGACTCAGC TCCAGATACT GGTGGACGAA 
TGTCGCCACG GAAAGAGCTA CTGCAAGCAG GTCCTGAGTC TCTACGAGCT CTCCAAGGAG 
CTGCAGTGTT CATACAGTGA GATCTCAGCC GAGGAGCCTC AGGCAGTGCT GTGTAAGGTG 
CTACTGTCCC AGCAGCCGGA GCGCTATAAG AAGGCCCAGG CCTTCATCAG TGTGCAGGGC 
CTGCAGCCTG ACACCGTGGC CCAGCTTGTA TCCACTGCCG TGGTCCAGGG CCTGTTGGCC 
TCTGCACAGG ACGGAGAGAC TGCAGAGAAG CAGATGTTCA GTCCAGAGGA AGGGAGAGAG 
TCCTTCTTGC AGCTGGCCAA GCTGTGTGGG GACCCCAACC TGGTGGGCAT CAAACTGCTG 
GACCACATCC CCACTGTGCC ACTGGGAGAG CTCGGCTGCA CGGTGGAGCT GTTGATCCTG 
GCCCACGATT GCTTCAGTCT GACCTGTAAC ATGGAGGGCA TAGTGAGGGT GCTGCAGGCT 
GCCCGCCACC TTAGCCACGC ACACCTGGCC CACAGCGAAC ACTACGGCCT GCTGGTGCGT 
CTCCTCACTG GCATCGGCAG ATACAATGAC ATGACCTACA TCTTCGACCT GCTCAACCAG 
AACCACCGCT TCGAGATGCT GCTCAGGAAA AAAGTGGAGT CGAACTTCCG GCTGAAGACG 
GCCCTGCTGG ACTACATCAA GCGGTGTTTG CCGGGGGACA GTGAGAAGTA CAACATGGTG 
GCCCTATGCT TCAGCATGTG CAGAGAGATA GGAGAGAACC ACGAGGGAGC CGCCCGCACC 
CAGCTCAAAC TGATCGAGTC CCAGCCTTGG GCAGTGACCC CTGAACTCAA AAGTGCCTTG 
ATAAAGGTTC TTACTCTCCT GAAGGATGCT GCAGAGAGCT ATTCTAAGGA CTCGTGTGTA 
CGCCAGGCAG CACGTTGTGT CAAAATGGCA AAGCTGACCA CACTCCAGCT CCACTTCCTG 
AACCACGGTC AAGACCAACG TGTCATAAAC CTGCGCCAAT CAGATCTCCT TGGTGCTATA 
GTGGCACTGC CCCGCTGTTA CCAGGCTTTT GTGTTGTCGG AGGCCTACGA CTACAGTCCA 
GACTGGGCGG AGGTGCTCTA CCAGAAGGTC ATCCTCAGTG GAGACTTTGC TTACCTAGAG 
GAGTTCCGAC TTCACAGGCC ACTCCCCGCC TGCCTGTTTG AGGACATATC ACAAAAATTG 
ACTCACAACA CGCCTCCGAG CAGTGCGGGC CAGAACCTGA AGAGACTATT GCAACACTGT 
GATGACGTTT ACACCTACTA CAAGCTTGCG TATGAACACA AGTTCTTCGA CGTGGCCAAC 
ATGTTACTTC AGGACTCCAA AACAAGCAGT TACCTAAATG ACAGGCTAGG TACTAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg11 ( XM_014123971.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013979446.1 Salmo salar spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X2, mRNA.

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CloneID	OAt32027
Clone ID Related Accession (Same CDS sequence)	XM_014123971.1
Accession Version	XM_014123971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012336573.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTATGA TAAATATGTT GCACAGATCT GAAACTCGAG ATAACTGTGC ATTAGAAGTC 
GCTCTCATCC CGGAGAGCAA CCTCTGTGTA AATGTTGTGG ACACAGCAAA AGCCAAAATG 
GCCCAAGGCT GCTCCTTGCT CTGCTGCTTG AGTTGTGCTG GGGGGCTGAC AGTCGGCGAC 
ATTTCAGAGA GCAAGGCACC GGGGAGGACG GTGGATGGCA CATATACAGA CTTTGCCTGG 
GAGGAGGTGA CAGTTTACAA TGGAGCCCGT GGCAGCCCTA GACTTCTGGC TGTGAGCTCT 
GAGTTTGAGC TGACACTGTA TGAACTGGAA GCAGAGGGCC CTACCTCTGG CCCTCTTGTC 
TCTGCCTCTG AGTTCACAGC AGATAAGCTA CTGCAGCTGG TCAAGGAGAA AGCCCTGAGT 
GTGACTGAGG TGGTGTCCTT GAGGCTGCTG TCCTTTGTGG ATGCCAGAAG CTTTCTCCTG 
CTCAACTCCA GTGTTGTGGT GCAGCTGCAG TGGCAGCAGG GGGCTGAACC GGAGACCCTG 
TCCTGCTGCA GCATCGTTCT GCCCCCAAAA GCCCCCCTGG ACACATGCAC TGACTACCAG 
CTCTGCAGAG GCATACTCTT TGTGTTGACC ACAGCTGGAG TCATATATGT GTTTGACAGT 
GCTGAGGGGA GGAAGCTGGC CAGTGTAGAC CTGCCCTCGT ACCACACTGC TGGGCTGGGG 
GAAGACGAGC CGGCCCCCTA CCTGGACTCC TTCTGTCTCC TCCAGGTGTC TCCTGACCTG 
GGTACTGCTG TTGCTGCCAC TCAGTCTAAC ACTGCCTTTG CTGTCGACCT CAACCACTAC 
TTCAGGATGT ACCCAGAGCA TCTGATGTGT CGGGTGGTCC CCAGTCGCCC TCCCCTCAGG 
CCCCAGGGTC CCTGTGACGA GGACAGCCTC TCCATCTCCA CCTACAGCCA CACTGTGCTG 
GGCACCTCCT TCAGCACTGA CCGGTCCTGG GAAGCTCGGC TGACTTCTCT GTACAACAAG 
GCCAAGGCTA CCCCTCCCTC TTCTCCTCAG ACTCCGGGGG CTCCTTGGTT TAAGGATTTC 
CCCCACATCG AGTGTGGGCG GGCAGCTATC GCTGCCAAGC TCTCCCAGAG CAGAGTCCCA 
TCAGGGGGAG CCACCATGCA GTTCTCTGTG CCCGACACAA CTGCCCCAGT GCTGCTCTCT 
GTCTCTGAAT TCTCCACTGT GTTGACCTTT GTCTCCCCTG GCAACAGCGA GACAACTGTG 
GCCTTCTGGG ATCTGCAGTC CCAGAGTGTT ACCTACCACC GGCCCACCTT GCCCACCGTG 
CCTATCCAGC GTACCGCAGA GGAGCAGCTC AGCCTGCTTC TGAAGACGTC AGGCCTGTCC 
CTGGTGCTGT TTGCTGTGTC ACAGGAGGAG CTGCTCAACA GACTAATGGT ATTTGGCAGT 
GCTGGCACAG TGGACTCTCT GTGCCACCTT AATGAATGGG GCCGCTGCTC CATCCCCATT 
CACGCCCTGC AGGCTGGTCT AAAGAACCAC CAGTTGGACA CAGTGGACTT CTTCCTGAAG 
AGCAAAAAGA ACATCCTGAC TCCACCCTCT GGGTATATTC TACCTGACCA ATCAGCTGCC 
ATCTCAACCC AGAGCCAGCT GAAGAATGTC CAGGATCTGT GTCCGGCTCT GGACCTTCTG 
CGTTGTGCCA TCAGAGACAA TCATACTGAG GCCCAGAGCC GACAATTTTC AGAACAGCTG 
CTCAGCATCA CCCTGGCCTT CCTCAACTCA CAAGTCAGGG CCGTCCTCTC TGCCACAGAG 
GAGCTGGATG AGAACTTGCA GGAGTGTGTG GAGATCTTGG ATAGTTACGT CTCGGATCTG 
CGCACCTTCA TGAAGAGGTT TCCCTGGCCA ACAGGTGGTG ACACTTCCAC CCTTGTCCCA 
TCCCCCATAC CTGTACCTGA GGAGGTGGAG AATGATGACT GGAGTTCCCT CTCTTCTGAG 
GAGGTGGTCG AGCGGGCAGT GCTGACCAAG CAGATTCCCC GGGCCCAGGC CTACCTGAGG 
AGCCAGAACA GGCCAGATCA GCGGCTGGAG GAGTTGAGGA GTACGGGGCT GCGTCTGGCC 
TTCAACTGCC TCACACACAG AGACCTGGAG CAGGCCACTG CACTGCTCAG GAACATGGGT 
TTCAACGTAA AGAAGCAACT GTATAACATA TGCCTCTACA CAGATGACCG GGACCTAAGG 
GACTTTGTGG TGGAGGAGTT GTCAAAGCAG AGCTATTTGT CTGATGAAGA GACACAGAGG 
GTGGAATTCA TCAAACAGAT TGAGGCTTTG TGTGCGGTGC CTGAGACGCG CTGCACAGAC 
CCTGTGGGCA GACAACGGGT TCCTCAGATG GCCCAGACAG ACCCAGAGTC TAAGAGCCTG 
CTGGAGGAGC TGGTAGACCA GAGAGGCTCC CAGTCAGAAC AGACACTGTG GAGTTCGCTG 
CGTCTGGACT GGGTCAGACA TTGGGACCAC AACACCCAGA GAGCCATCCT GCTGTCACGC 
CTACAAGACT CGGTTGTCAG TTCCTGTGAC CCGGGGGTCC TGTGGTCATA CCTCACCTCC 
CACCACGACC AGCGGAGGGT AACCGAGTGG ATTGAGAGCC TGGCCGCCCA GAACGGTGAC 
CCTTGCAGCG CCCCCCAGTG GCCCGCCCTC ACGGCAGAGG TGGTCAACAA CAACACCCGG 
TGTAGCGCCC ACATGAGGAA CCACATCCTC TACATGTTGG CCAGGAGAGG AGTGTTCATC 
CAGGCCGAGA TGGCTGATTT TGAGCATCTG TTGTGGAGGC TGGGCCAGGC AGGTGGGGTG 
ATGCAGGCCA GCCCACCCAT GCCCCAGTAC CGCTCCCCTC AAGGACACGA CTTACACACA 
CGCTTCATCC ACCACTGCCT GGACAACGGC CTTCGCTACC TGCTCTACAC CTACCTGGAG 
CACAACAGAT TGACCCCCAG GAATTGCCCT GTCCTTGCTG ATAATGGTCT ATATGAAAGT 
CACCCGTGGT TTGAAATGCT GGTGAAAATG CAAGAGATTA CCAGAGACCT CTCAGATCCA 
GAACGCGTGT TCCAGGCCAG TCTGACCAGT GCCCAGGTCC TCATCCCAGG CAGCCAGGCC 
ATGGTTAGCA GCATGCTCCT GGAAGGACAC AGTCTTCTGG CCCTCGCTAC CATCATGTTT 
GCCCCGGGTG GCATCGACCA GGTGGTGAGT CAGGGGGAGA GGGCTAGGAG CTCTGTGTCT 
AGGGTGGACC CCCAGCTACT GAAGATGGCC CTGAGCCCCT ACCCCAAGCT CAAGGCAGCG 
CTCTTCCCTG TGAGCGGACC ACGAGGGACC ACCGCCACCT CATCCGATAT CTCCGTCTAC 
CACCTCATGC AGTCTTTACA TCCATTGGAT CCATCTCGAC TGTTCGGGTG GCAGTCTGCC 
AATACACTAG GCTCCACCGA GACCTCAGTG GAGCTGCCCC ATTTCTCTAG TCCTCACCTC 
GTCAGTAAAT ACGCCCTCAT TGAGAACCTC GACTTCCTGT ACTATCTGCG TCACGGACGA 
CCATCGTTTG CCTACGGAAC GTTTCTCGTC CAACAGCTAG CCAACTCCAA CGAAGTGAAG 
TCGCAGCTCA GTCATGCGGC GGAGCAGGCC TACAGCCTGG CCATGCTGTA TTTCAGCGTT 
CCCTCTGTGG CAGCTGCATG TGTGTGTTTC TGCGAGCTCC TGGGTGTATG TAGCCTAAAG 
TTGCGGGTGG ACCTCAAAGC CCTCAGTCTG ATCCTCCGAC TCCGGAGCCA GAGGCCTGGA 
GGGACCAGCA CATTCTCCCT TAAAGACTTA CTGGCTCCTA AAGGCAGTAA GCTGGTGGAG 
GTGGAGAGGG AGACAGCGGA GGAGCTGCTC TTTGACTTGG AGGCAGCTGT GAAGGACAGT 
TTGGAGAGGA AGGGCATCAG CAGGTCATCC TATGAGGCAG GTCAGGAGTG GGCTCTGCCC 
ATACAGTTTT GCCAGCTACA TGCCCTGCCC CTGAGCAGCG TGTACCCACA CGACTGTGCA 
GCCGACAGCC AGTGGCTACA CTTCCTGCTC TTTGTCCAGC TGCACAGCTA CCCCCCTCAG 
CAGGTGAGGT CCCTGGCGAC CCATTTCAGC CCAGCCCTGC AGGCCCACCT GACCCTGGCC 
TTCCAGAACC TGCAGCTCAG CTCTCAGTGG AGGGTGGGGG AGGTGGAAGA CAGGGAGGCC 
CAGGGTCAAG TCACCCTGCC CAGCGAGGAA GCCTCCGACC CCCCCAAGGA GCTGTTCCAG 
GTGCTGCTGC AGAGCCAGGA GCAGGCCAGC CCCTGGAGAC ACCTCCTGGG GGAGGCCCTG 
GCACAGCACT GCCCCACGCT CACCACACTA GCTGCCTGTC ATCAGGAAGC CGACCTGCTC 
CAGTGCCTCT GCGTCTGGGT CCTGACCTCT GTGGATGAGG TCATTGCCAC AGAGGCTACC 
GCCCACCTGG AGGAGAGCCC GCGACGTCAT GAGTGGAACC TCCACGATCT GTCAATCATC 
TGGAAGACAC TGCTGAGGAA ATGCAGGGTC AGGCCTCTCA TACGAGGCTT CCAGCTCTTC 
CAGAGGGACT CTCCCCTGAT CCATGTGCTG CTCATGTACG AGCTGTGCTA CGACTACAAA 
AACTACCCTG AAGCCAAGAA GAAACTCATT GAGTTCCAGA AATGCCTGAT CACGCTGAGG 
AGCAGTGGTG CTCATGCGCC AGCGGGCATC CCTCTCCAGT GGGTGGAGTG CCAGGCCTCA 
GTCCTCCTGT TGACCATGAT GCAACAGTGC TCCACACAAT ACGAGCTGCG CAGACTACTG 
CAGCTGCTCG CAGACATGGA GCGCCTGCTC AAGTCTAACG GCCCGGACTT CAAGAAGCTC 
AGCTTCCTCA GCCAGGTTCT CCAGGACACC TCGGTGTCTC TCTCTCCCAG GCTGCTTGAG 
AGCTATTCCC CTGAAATCCT GCAGGGAGAG TGCCAGAGCA TGTTGGAGAA GCTGCAAGAC 
AGCGGCCTGT TCCACAAGGC CCGCCAGGTA GCTGAGCTGG CAGGGCTGCC CATTGACAGC 
CTGGTCATCA ACGAGTTACT CCAAGACCTC AGTGCCCTGA AGGCCAAGCG GCACTGGGAG 
AGGAGGGAGA CCCGGGTGTC CTTCTGGAGG AAATGCCACG AGCAGTTCAG GACTAACAGC 
ACTGGCCCTG GGGCCGCCTC AGAGTTCTTC CTGGCCCAGG CAGGTGCCTC TGAGCCTCAC 
CGGGGCTCCG TGGCCGAGAC AGAGCTGCTG TGTGTCCAGG AGAGGTGCCT TCTCCTCAGC 
CTGGCCGGCC ACTGGCTGTC CTGGCTCACC CTAGCTCCCC TGGCACGTCT CGAGGACCTG 
GAGAAGCAGC TGTGGACAAG CCGAGTGCGC CAGCAAGTCC TGCTCACCGC CATGGAGAAG 
GAGAGCATGT TCGCCCTCCC GGCGCCCACC ATAGCCCCCG AGGAAAACTC CTATGAGGCC 
CTGATCAGGG AGTTCTCCTT CTCCAAGATG GCAGTGCTCA ACGAGTCCAG GTACCTGAGC 
TTAGACGGCC TGCCCAGCTT CTCCCCTGAG GAAGAGGGTG GGGAGAAGAC TGGGTCGGAG 
GAGCAGGGTG CTCTGGCTAT CCTGGTGGGG CAGCTGTTGG ACGAAGGGTG TATCCACGAA 
GCGAGCCGTG TGTGCCGTTA CTTCTCCTTC TGCTCCCGGG ACATGTGGGT AGTGCTGCAC 
TGCCGAGGCC TGGCCTCAGG CGAACTCCAG GCTGGCCTGC AGGACATGCT CACCTTTGAG 
GGCCTGCCCA AGAAGAGCAT TCCCAGCTCA ACTAGTTTTA GCAGCCTGTC CTCCTTTGTG 
ATGGTGCCCT CTCCAGAGGA TCAAACGGTG ACTCAGCTCC AGATACTGGT GGACGAATGT 
CGCCACGGAA AGAGCTACTG CAAGCAGGTC CTGAGTCTCT ACGAGCTCTC CAAGGAGCTG 
CAGTGTTCAT ACAGTGAGAT CTCAGCCGAG GAGCCTCAGG CAGTGCTGTG TAAGGTGCTA 
CTGTCCCAGC AGCCGGAGCG CTATAAGAAG GCCCAGGCCT TCATCAGTGT GCAGGGCCTG 
CAGCCTGACA CCGTGGCCCA GCTTGTATCC ACTGCCGTGG TCCAGGGCCT GTTGGCCTCT 
GCACAGGACG GAGAGACTGC AGAGAAGCAG ATGTTCAGTC CAGAGGAAGG GAGAGAGTCC 
TTCTTGCAGC TGGCCAAGCT GTGTGGGGAC CCCAACCTGG TGGGCATCAA ACTGCTGGAC 
CACATCCCCA CTGTGCCACT GGGAGAGCTC GGCTGCACGG TGGAGCTGTT GATCCTGGCC 
CACGATTGCT TCAGTCTGAC CTGTAACATG GAGGGCATAG TGAGGGTGCT GCAGGCTGCC 
CGCCACCTTA GCCACGCACA CCTGGCCCAC AGCGAACACT ACGGCCTGCT GGTGCGTCTC 
CTCACTGGCA TCGGCAGATA CAATGACATG ACCTACATCT TCGACCTGCT CAACCAGAAC 
CACCGCTTCG AGATGCTGCT CAGGAAAAAA GTGGAGTCGA ACTTCCGGCT GAAGACGGCC 
CTGCTGGACT ACATCAAGCG GTGTTTGCCG GGGGACAGTG AGAAGTACAA CATGGTGGCC 
CTATGCTTCA GCATGTGCAG AGAGATAGGA GAGAACCACG AGGGAGCCGC CCGCACCCAG 
CTCAAACTGA TCGAGTCCCA GCCTTGGGCA GTGACCCCTG AACTCAAAAG TGCCTTGATA 
AAGGTTCTTA CTCTCCTGAA GGATGCTGCA GAGAGCTATT CTAAGGACTC GTGTGTACGC 
CAGGCAGCAC GTTGTGTCAA AATGGCAAAG CTGACCACAC TCCAGCTCCA CTTCCTGAAC 
CACGGTCAAG ACCAACGTGT CATAAACCTG CGCCAATCAG ATCTCCTTGG TGCTATAGTG 
GCACTGCCCC GCTGTTACCA GGCTTTTGTG TTGTCGGAGG CCTACGACTA CAGTCCAGAC 
TGGGCGGAGG TGCTCTACCA GAAGGTCATC CTCAGTGGAG ACTTTGCTTA CCTAGAGGAG 
TTCCGACTTC ACAGGCCACT CCCCGCCTGC CTGTTTGAGG ACATATCACA AAAATTGACT 
CACAACACGC CTCCGAGCAG TGCGGGCCAG AACCTGAAGA GACTATTGCA ACACTGTGAT 
GACGTTTACA CCTACTACAA GCTTGCGTAT GAACACAAGT TCTTCGACGT GGCCAACATG 
TTACTTCAGG ACTCCAAAAC AAGCAGTTAC CTAAATGACA GGCTAGGTAC TAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013979446.1
CDS	220..7476
Translation	MIMINMLHRSETRDNCALEVALIPESNLCVNVVDTAKAKMAQGCSLLCCLSCAGGLTVGDISESKAPGRTVDGTYTDFAWEEVTVYNGARGSPRLLAVSSEFELTLYELEAEGPTSGPLVSASEFTADKLLQLVKEKALSVTEVVSLRLLSFVDARSFLLLNSSVVVQLQWQQGAEPETLSCCSIVLPPKAPLDTCTDYQLCRGILFVLTTAGVIYVFDSAEGRKLASVDLPSYHTAGLGEDEPAPYLDSFCLLQVSPDLGTAVAATQSNTAFAVDLNHYFRMYPEHLMCRVVPSRPPLRPQGPCDEDSLSISTYSHTVLGTSFSTDRSWEARLTSLYNKAKATPPSSPQTPGAPWFKDFPHIECGRAAIAAKLSQSRVPSGGATMQFSVPDTTAPVLLSVSEFSTVLTFVSPGNSETTVAFWDLQSQSVTYHRPTLPTVPIQRTAEEQLSLLLKTSGLSLVLFAVSQEELLNRLMVFGSAGTVDSLCHLNEWGRCSIPIHALQAGLKNHQLDTVDFFLKSKKNILTPPSGYILPDQSAAISTQSQLKNVQDLCPALDLLRCAIRDNHTEAQSRQFSEQLLSITLAFLNSQVRAVLSATEELDENLQECVEILDSYVSDLRTFMKRFPWPTGGDTSTLVPSPIPVPEEVENDDWSSLSSEEVVERAVLTKQIPRAQAYLRSQNRPDQRLEELRSTGLRLAFNCLTHRDLEQATALLRNMGFNVKKQLYNICLYTDDRDLRDFVVEELSKQSYLSDEETQRVEFIKQIEALCAVPETRCTDPVGRQRVPQMAQTDPESKSLLEELVDQRGSQSEQTLWSSLRLDWVRHWDHNTQRAILLSRLQDSVVSSCDPGVLWSYLTSHHDQRRVTEWIESLAAQNGDPCSAPQWPALTAEVVNNNTRCSAHMRNHILYMLARRGVFIQAEMADFEHLLWRLGQAGGVMQASPPMPQYRSPQGHDLHTRFIHHCLDNGLRYLLYTYLEHNRLTPRNCPVLADNGLYESHPWFEMLVKMQEITRDLSDPERVFQASLTSAQVLIPGSQAMVSSMLLEGHSLLALATIMFAPGGIDQVVSQGERARSSVSRVDPQLLKMALSPYPKLKAALFPVSGPRGTTATSSDISVYHLMQSLHPLDPSRLFGWQSANTLGSTETSVELPHFSSPHLVSKYALIENLDFLYYLRHGRPSFAYGTFLVQQLANSNEVKSQLSHAAEQAYSLAMLYFSVPSVAAACVCFCELLGVCSLKLRVDLKALSLILRLRSQRPGGTSTFSLKDLLAPKGSKLVEVERETAEELLFDLEAAVKDSLERKGISRSSYEAGQEWALPIQFCQLHALPLSSVYPHDCAADSQWLHFLLFVQLHSYPPQQVRSLATHFSPALQAHLTLAFQNLQLSSQWRVGEVEDREAQGQVTLPSEEASDPPKELFQVLLQSQEQASPWRHLLGEALAQHCPTLTTLAACHQEADLLQCLCVWVLTSVDEVIATEATAHLEESPRRHEWNLHDLSIIWKTLLRKCRVRPLIRGFQLFQRDSPLIHVLLMYELCYDYKNYPEAKKKLIEFQKCLITLRSSGAHAPAGIPLQWVECQASVLLLTMMQQCSTQYELRRLLQLLADMERLLKSNGPDFKKLSFLSQVLQDTSVSLSPRLLESYSPEILQGECQSMLEKLQDSGLFHKARQVAELAGLPIDSLVINELLQDLSALKAKRHWERRETRVSFWRKCHEQFRTNSTGPGAASEFFLAQAGASEPHRGSVAETELLCVQERCLLLSLAGHWLSWLTLAPLARLEDLEKQLWTSRVRQQVLLTAMEKESMFALPAPTIAPEENSYEALIREFSFSKMAVLNESRYLSLDGLPSFSPEEEGGEKTGSEEQGALAILVGQLLDEGCIHEASRVCRYFSFCSRDMWVVLHCRGLASGELQAGLQDMLTFEGLPKKSIPSSTSFSSLSSFVMVPSPEDQTVTQLQILVDECRHGKSYCKQVLSLYELSKELQCSYSEISAEEPQAVLCKVLLSQQPERYKKAQAFISVQGLQPDTVAQLVSTAVVQGLLASAQDGETAEKQMFSPEEGRESFLQLAKLCGDPNLVGIKLLDHIPTVPLGELGCTVELLILAHDCFSLTCNMEGIVRVLQAARHLSHAHLAHSEHYGLLVRLLTGIGRYNDMTYIFDLLNQNHRFEMLLRKKVESNFRLKTALLDYIKRCLPGDSEKYNMVALCFSMCREIGENHEGAARTQLKLIESQPWAVTPELKSALIKVLTLLKDAAESYSKDSCVRQAARCVKMAKLTTLQLHFLNHGQDQRVINLRQSDLLGAIVALPRCYQAFVLSEAYDYSPDWAEVLYQKVILSGDFAYLEEFRLHRPLPACLFEDISQKLTHNTPPSSAGQNLKRLLQHCDDVYTYYKLAYEHKFFDVANMLLQDSKTSSYLNDRLGTR

Target ORF information:

RefSeq Version	XM_014123971.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014123971.1

ORF Insert Sequence:

ATGATTATGA TAAATATGTT GCACAGATCT GAAACTCGAG ATAACTGTGC ATTAGAAGTC 
GCTCTCATCC CGGAGAGCAA CCTCTGTGTA AATGTTGTGG ACACAGCAAA AGCCAAAATG 
GCCCAAGGCT GCTCCTTGCT CTGCTGCTTG AGTTGTGCTG GGGGGCTGAC AGTCGGCGAC 
ATTTCAGAGA GCAAGGCACC GGGGAGGACG GTGGATGGCA CATATACAGA CTTTGCCTGG 
GAGGAGGTGA CAGTTTACAA TGGAGCCCGT GGCAGCCCTA GACTTCTGGC TGTGAGCTCT 
GAGTTTGAGC TGACACTGTA TGAACTGGAA GCAGAGGGCC CTACCTCTGG CCCTCTTGTC 
TCTGCCTCTG AGTTCACAGC AGATAAGCTA CTGCAGCTGG TCAAGGAGAA AGCCCTGAGT 
GTGACTGAGG TGGTGTCCTT GAGGCTGCTG TCCTTTGTGG ATGCCAGAAG CTTTCTCCTG 
CTCAACTCCA GTGTTGTGGT GCAGCTGCAG TGGCAGCAGG GGGCTGAACC GGAGACCCTG 
TCCTGCTGCA GCATCGTTCT GCCCCCAAAA GCCCCCCTGG ACACATGCAC TGACTACCAG 
CTCTGCAGAG GCATACTCTT TGTGTTGACC ACAGCTGGAG TCATATATGT GTTTGACAGT 
GCTGAGGGGA GGAAGCTGGC CAGTGTAGAC CTGCCCTCGT ACCACACTGC TGGGCTGGGG 
GAAGACGAGC CGGCCCCCTA CCTGGACTCC TTCTGTCTCC TCCAGGTGTC TCCTGACCTG 
GGTACTGCTG TTGCTGCCAC TCAGTCTAAC ACTGCCTTTG CTGTCGACCT CAACCACTAC 
TTCAGGATGT ACCCAGAGCA TCTGATGTGT CGGGTGGTCC CCAGTCGCCC TCCCCTCAGG 
CCCCAGGGTC CCTGTGACGA GGACAGCCTC TCCATCTCCA CCTACAGCCA CACTGTGCTG 
GGCACCTCCT TCAGCACTGA CCGGTCCTGG GAAGCTCGGC TGACTTCTCT GTACAACAAG 
GCCAAGGCTA CCCCTCCCTC TTCTCCTCAG ACTCCGGGGG CTCCTTGGTT TAAGGATTTC 
CCCCACATCG AGTGTGGGCG GGCAGCTATC GCTGCCAAGC TCTCCCAGAG CAGAGTCCCA 
TCAGGGGGAG CCACCATGCA GTTCTCTGTG CCCGACACAA CTGCCCCAGT GCTGCTCTCT 
GTCTCTGAAT TCTCCACTGT GTTGACCTTT GTCTCCCCTG GCAACAGCGA GACAACTGTG 
GCCTTCTGGG ATCTGCAGTC CCAGAGTGTT ACCTACCACC GGCCCACCTT GCCCACCGTG 
CCTATCCAGC GTACCGCAGA GGAGCAGCTC AGCCTGCTTC TGAAGACGTC AGGCCTGTCC 
CTGGTGCTGT TTGCTGTGTC ACAGGAGGAG CTGCTCAACA GACTAATGGT ATTTGGCAGT 
GCTGGCACAG TGGACTCTCT GTGCCACCTT AATGAATGGG GCCGCTGCTC CATCCCCATT 
CACGCCCTGC AGGCTGGTCT AAAGAACCAC CAGTTGGACA CAGTGGACTT CTTCCTGAAG 
AGCAAAAAGA ACATCCTGAC TCCACCCTCT GGGTATATTC TACCTGACCA ATCAGCTGCC 
ATCTCAACCC AGAGCCAGCT GAAGAATGTC CAGGATCTGT GTCCGGCTCT GGACCTTCTG 
CGTTGTGCCA TCAGAGACAA TCATACTGAG GCCCAGAGCC GACAATTTTC AGAACAGCTG 
CTCAGCATCA CCCTGGCCTT CCTCAACTCA CAAGTCAGGG CCGTCCTCTC TGCCACAGAG 
GAGCTGGATG AGAACTTGCA GGAGTGTGTG GAGATCTTGG ATAGTTACGT CTCGGATCTG 
CGCACCTTCA TGAAGAGGTT TCCCTGGCCA ACAGGTGGTG ACACTTCCAC CCTTGTCCCA 
TCCCCCATAC CTGTACCTGA GGAGGTGGAG AATGATGACT GGAGTTCCCT CTCTTCTGAG 
GAGGTGGTCG AGCGGGCAGT GCTGACCAAG CAGATTCCCC GGGCCCAGGC CTACCTGAGG 
AGCCAGAACA GGCCAGATCA GCGGCTGGAG GAGTTGAGGA GTACGGGGCT GCGTCTGGCC 
TTCAACTGCC TCACACACAG AGACCTGGAG CAGGCCACTG CACTGCTCAG GAACATGGGT 
TTCAACGTAA AGAAGCAACT GTATAACATA TGCCTCTACA CAGATGACCG GGACCTAAGG 
GACTTTGTGG TGGAGGAGTT GTCAAAGCAG AGCTATTTGT CTGATGAAGA GACACAGAGG 
GTGGAATTCA TCAAACAGAT TGAGGCTTTG TGTGCGGTGC CTGAGACGCG CTGCACAGAC 
CCTGTGGGCA GACAACGGGT TCCTCAGATG GCCCAGACAG ACCCAGAGTC TAAGAGCCTG 
CTGGAGGAGC TGGTAGACCA GAGAGGCTCC CAGTCAGAAC AGACACTGTG GAGTTCGCTG 
CGTCTGGACT GGGTCAGACA TTGGGACCAC AACACCCAGA GAGCCATCCT GCTGTCACGC 
CTACAAGACT CGGTTGTCAG TTCCTGTGAC CCGGGGGTCC TGTGGTCATA CCTCACCTCC 
CACCACGACC AGCGGAGGGT AACCGAGTGG ATTGAGAGCC TGGCCGCCCA GAACGGTGAC 
CCTTGCAGCG CCCCCCAGTG GCCCGCCCTC ACGGCAGAGG TGGTCAACAA CAACACCCGG 
TGTAGCGCCC ACATGAGGAA CCACATCCTC TACATGTTGG CCAGGAGAGG AGTGTTCATC 
CAGGCCGAGA TGGCTGATTT TGAGCATCTG TTGTGGAGGC TGGGCCAGGC AGGTGGGGTG 
ATGCAGGCCA GCCCACCCAT GCCCCAGTAC CGCTCCCCTC AAGGACACGA CTTACACACA 
CGCTTCATCC ACCACTGCCT GGACAACGGC CTTCGCTACC TGCTCTACAC CTACCTGGAG 
CACAACAGAT TGACCCCCAG GAATTGCCCT GTCCTTGCTG ATAATGGTCT ATATGAAAGT 
CACCCGTGGT TTGAAATGCT GGTGAAAATG CAAGAGATTA CCAGAGACCT CTCAGATCCA 
GAACGCGTGT TCCAGGCCAG TCTGACCAGT GCCCAGGTCC TCATCCCAGG CAGCCAGGCC 
ATGGTTAGCA GCATGCTCCT GGAAGGACAC AGTCTTCTGG CCCTCGCTAC CATCATGTTT 
GCCCCGGGTG GCATCGACCA GGTGGTGAGT CAGGGGGAGA GGGCTAGGAG CTCTGTGTCT 
AGGGTGGACC CCCAGCTACT GAAGATGGCC CTGAGCCCCT ACCCCAAGCT CAAGGCAGCG 
CTCTTCCCTG TGAGCGGACC ACGAGGGACC ACCGCCACCT CATCCGATAT CTCCGTCTAC 
CACCTCATGC AGTCTTTACA TCCATTGGAT CCATCTCGAC TGTTCGGGTG GCAGTCTGCC 
AATACACTAG GCTCCACCGA GACCTCAGTG GAGCTGCCCC ATTTCTCTAG TCCTCACCTC 
GTCAGTAAAT ACGCCCTCAT TGAGAACCTC GACTTCCTGT ACTATCTGCG TCACGGACGA 
CCATCGTTTG CCTACGGAAC GTTTCTCGTC CAACAGCTAG CCAACTCCAA CGAAGTGAAG 
TCGCAGCTCA GTCATGCGGC GGAGCAGGCC TACAGCCTGG CCATGCTGTA TTTCAGCGTT 
CCCTCTGTGG CAGCTGCATG TGTGTGTTTC TGCGAGCTCC TGGGTGTATG TAGCCTAAAG 
TTGCGGGTGG ACCTCAAAGC CCTCAGTCTG ATCCTCCGAC TCCGGAGCCA GAGGCCTGGA 
GGGACCAGCA CATTCTCCCT TAAAGACTTA CTGGCTCCTA AAGGCAGTAA GCTGGTGGAG 
GTGGAGAGGG AGACAGCGGA GGAGCTGCTC TTTGACTTGG AGGCAGCTGT GAAGGACAGT 
TTGGAGAGGA AGGGCATCAG CAGGTCATCC TATGAGGCAG GTCAGGAGTG GGCTCTGCCC 
ATACAGTTTT GCCAGCTACA TGCCCTGCCC CTGAGCAGCG TGTACCCACA CGACTGTGCA 
GCCGACAGCC AGTGGCTACA CTTCCTGCTC TTTGTCCAGC TGCACAGCTA CCCCCCTCAG 
CAGGTGAGGT CCCTGGCGAC CCATTTCAGC CCAGCCCTGC AGGCCCACCT GACCCTGGCC 
TTCCAGAACC TGCAGCTCAG CTCTCAGTGG AGGGTGGGGG AGGTGGAAGA CAGGGAGGCC 
CAGGGTCAAG TCACCCTGCC CAGCGAGGAA GCCTCCGACC CCCCCAAGGA GCTGTTCCAG 
GTGCTGCTGC AGAGCCAGGA GCAGGCCAGC CCCTGGAGAC ACCTCCTGGG GGAGGCCCTG 
GCACAGCACT GCCCCACGCT CACCACACTA GCTGCCTGTC ATCAGGAAGC CGACCTGCTC 
CAGTGCCTCT GCGTCTGGGT CCTGACCTCT GTGGATGAGG TCATTGCCAC AGAGGCTACC 
GCCCACCTGG AGGAGAGCCC GCGACGTCAT GAGTGGAACC TCCACGATCT GTCAATCATC 
TGGAAGACAC TGCTGAGGAA ATGCAGGGTC AGGCCTCTCA TACGAGGCTT CCAGCTCTTC 
CAGAGGGACT CTCCCCTGAT CCATGTGCTG CTCATGTACG AGCTGTGCTA CGACTACAAA 
AACTACCCTG AAGCCAAGAA GAAACTCATT GAGTTCCAGA AATGCCTGAT CACGCTGAGG 
AGCAGTGGTG CTCATGCGCC AGCGGGCATC CCTCTCCAGT GGGTGGAGTG CCAGGCCTCA 
GTCCTCCTGT TGACCATGAT GCAACAGTGC TCCACACAAT ACGAGCTGCG CAGACTACTG 
CAGCTGCTCG CAGACATGGA GCGCCTGCTC AAGTCTAACG GCCCGGACTT CAAGAAGCTC 
AGCTTCCTCA GCCAGGTTCT CCAGGACACC TCGGTGTCTC TCTCTCCCAG GCTGCTTGAG 
AGCTATTCCC CTGAAATCCT GCAGGGAGAG TGCCAGAGCA TGTTGGAGAA GCTGCAAGAC 
AGCGGCCTGT TCCACAAGGC CCGCCAGGTA GCTGAGCTGG CAGGGCTGCC CATTGACAGC 
CTGGTCATCA ACGAGTTACT CCAAGACCTC AGTGCCCTGA AGGCCAAGCG GCACTGGGAG 
AGGAGGGAGA CCCGGGTGTC CTTCTGGAGG AAATGCCACG AGCAGTTCAG GACTAACAGC 
ACTGGCCCTG GGGCCGCCTC AGAGTTCTTC CTGGCCCAGG CAGGTGCCTC TGAGCCTCAC 
CGGGGCTCCG TGGCCGAGAC AGAGCTGCTG TGTGTCCAGG AGAGGTGCCT TCTCCTCAGC 
CTGGCCGGCC ACTGGCTGTC CTGGCTCACC CTAGCTCCCC TGGCACGTCT CGAGGACCTG 
GAGAAGCAGC TGTGGACAAG CCGAGTGCGC CAGCAAGTCC TGCTCACCGC CATGGAGAAG 
GAGAGCATGT TCGCCCTCCC GGCGCCCACC ATAGCCCCCG AGGAAAACTC CTATGAGGCC 
CTGATCAGGG AGTTCTCCTT CTCCAAGATG GCAGTGCTCA ACGAGTCCAG GTACCTGAGC 
TTAGACGGCC TGCCCAGCTT CTCCCCTGAG GAAGAGGGTG GGGAGAAGAC TGGGTCGGAG 
GAGCAGGGTG CTCTGGCTAT CCTGGTGGGG CAGCTGTTGG ACGAAGGGTG TATCCACGAA 
GCGAGCCGTG TGTGCCGTTA CTTCTCCTTC TGCTCCCGGG ACATGTGGGT AGTGCTGCAC 
TGCCGAGGCC TGGCCTCAGG CGAACTCCAG GCTGGCCTGC AGGACATGCT CACCTTTGAG 
GGCCTGCCCA AGAAGAGCAT TCCCAGCTCA ACTAGTTTTA GCAGCCTGTC CTCCTTTGTG 
ATGGTGCCCT CTCCAGAGGA TCAAACGGTG ACTCAGCTCC AGATACTGGT GGACGAATGT 
CGCCACGGAA AGAGCTACTG CAAGCAGGTC CTGAGTCTCT ACGAGCTCTC CAAGGAGCTG 
CAGTGTTCAT ACAGTGAGAT CTCAGCCGAG GAGCCTCAGG CAGTGCTGTG TAAGGTGCTA 
CTGTCCCAGC AGCCGGAGCG CTATAAGAAG GCCCAGGCCT TCATCAGTGT GCAGGGCCTG 
CAGCCTGACA CCGTGGCCCA GCTTGTATCC ACTGCCGTGG TCCAGGGCCT GTTGGCCTCT 
GCACAGGACG GAGAGACTGC AGAGAAGCAG ATGTTCAGTC CAGAGGAAGG GAGAGAGTCC 
TTCTTGCAGC TGGCCAAGCT GTGTGGGGAC CCCAACCTGG TGGGCATCAA ACTGCTGGAC 
CACATCCCCA CTGTGCCACT GGGAGAGCTC GGCTGCACGG TGGAGCTGTT GATCCTGGCC 
CACGATTGCT TCAGTCTGAC CTGTAACATG GAGGGCATAG TGAGGGTGCT GCAGGCTGCC 
CGCCACCTTA GCCACGCACA CCTGGCCCAC AGCGAACACT ACGGCCTGCT GGTGCGTCTC 
CTCACTGGCA TCGGCAGATA CAATGACATG ACCTACATCT TCGACCTGCT CAACCAGAAC 
CACCGCTTCG AGATGCTGCT CAGGAAAAAA GTGGAGTCGA ACTTCCGGCT GAAGACGGCC 
CTGCTGGACT ACATCAAGCG GTGTTTGCCG GGGGACAGTG AGAAGTACAA CATGGTGGCC 
CTATGCTTCA GCATGTGCAG AGAGATAGGA GAGAACCACG AGGGAGCCGC CCGCACCCAG 
CTCAAACTGA TCGAGTCCCA GCCTTGGGCA GTGACCCCTG AACTCAAAAG TGCCTTGATA 
AAGGTTCTTA CTCTCCTGAA GGATGCTGCA GAGAGCTATT CTAAGGACTC GTGTGTACGC 
CAGGCAGCAC GTTGTGTCAA AATGGCAAAG CTGACCACAC TCCAGCTCCA CTTCCTGAAC 
CACGGTCAAG ACCAACGTGT CATAAACCTG CGCCAATCAG ATCTCCTTGG TGCTATAGTG 
GCACTGCCCC GCTGTTACCA GGCTTTTGTG TTGTCGGAGG CCTACGACTA CAGTCCAGAC 
TGGGCGGAGG TGCTCTACCA GAAGGTCATC CTCAGTGGAG ACTTTGCTTA CCTAGAGGAG 
TTCCGACTTC ACAGGCCACT CCCCGCCTGC CTGTTTGAGG ACATATCACA AAAATTGACT 
CACAACACGC CTCCGAGCAG TGCGGGCCAG AACCTGAAGA GACTATTGCA ACACTGTGAT 
GACGTTTACA CCTACTACAA GCTTGCGTAT GAACACAAGT TCTTCGACGT GGCCAACATG 
TTACTTCAGG ACTCCAAAAC AAGCAGTTAC CTAAATGACA GGCTAGGTAC TAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg11 ( XM_014123972.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_013979447.1 Salmo salar spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X3, mRNA.

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CloneID	OAt32028
Clone ID Related Accession (Same CDS sequence)	XM_014123972.1
Accession Version	XM_014123972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	spatacsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012336573.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTATGA TAAATATGTT GCACAGATCT GAAACTCGAG ATAACTGTGC ATTAGAAGTC 
GCTCTCATCC CGGAGAGCAA CCTCTGTGTA AATGTTGTGG ACACAGCAAA AGCCAAAATG 
GCCCAAGGCT GCTCCTTGCT CTGCTGCTTG AGTTGTGCTG GGGGGCTGAC AGTCGGCGAC 
ATTTCAGAGA GCAAGGCACC GGGGAGGACG GTGGATGGCA CATATACAGA CTTTGCCTGG 
GAGGAGGTGA CAGTTTACAA TGGAGCCCGT GGCAGCCCTA GACTTCTGGC TGTGAGCTCT 
GAGTTTGAGC TGACACTGTA TGAACTGGAA GCAGAGGGCC CTACCTCTGG CCCTCTTGTC 
TCTGCCTCTG AGTTCACAGC AGATAAGCTA CTGCAGCTGG TCAAGGAGAA AGCCCTGAGT 
GTGACTGAGG TGGTGTCCTT GAGGCTGCTG TCCTTTGTGG ATGCCAGAAG CTTTCTCCTG 
CTCAACTCCA GTGTTGTGGT GCAGCTGCAG TGGCAGCAGG GGGCTGAACC GGAGACCCTG 
TCCTGCTGCA GCATCGTTCT GCCCCCAAAA GCCCCCCTGG ACACATGCAC TGACTACCAG 
CTCTGCAGAG GCATACTCTT TGTGTTGACC ACAGCTGGAG TCATATATGT GTTTGACAGT 
GCTGAGGGGA GGAAGCTGGC CAGTGTAGAC CTGCCCTCGT ACCACACTGC TGGGCTGGGG 
GAAGACGAGC CGGCCCCCTA CCTGGACTCC TTCTGTCTCC TCCAGGTGTC TCCTGACCTG 
GGTACTGCTG TTGCTGCCAC TCAGTCTAAC ACTGCCTTTG CTGTCGACCT CAACCACTAC 
TTCAGGATGT ACCCAGAGCA TCTGATGTGT CGGGTGGTCC CCAGTCGCCC TCCCCTCAGG 
CCCCAGGGTC CCTGTGACGA GGACAGCCTC TCCATCTCCA CCTACAGCCA CACTGTGCTG 
GGCACCTCCT TCAGCACTGA CCGGTCCTGG GAAGCTCGGC TGACTTCTCT GTACAACAAG 
GCCAAGGCTA CCCCTCCCTC TTCTCCTCAG ACTCCGGGGG CTCCTTGGTT TAAGGATTTC 
CCCCACATCG AGTGTGGGCG GGCAGCTATC GCTGCCAAGC TCTCCCAGAG CAGAGTCCCA 
TCAGGGGGAG CCACCATGCA GTTCTCTGTG CCCGACACAA CTGCCCCAGT GCTGCTCTCT 
GTCTCTGAAT TCTCCACTGT GTTGACCTTT GTCTCCCCTG GCAACAGCGA GACAACTGTG 
GCCTTCTGGG ATCTGCAGTC CCAGAGTGTT ACCTACCACC GGCCCACCTT GCCCACCGTG 
CCTATCCAGC GTACCGCAGA GGAGCAGCTC AGCCTGCTTC TGAAGACGTC AGGCCTGTCC 
CTGGTGCTGT TTGCTGTGTC ACAGGAGGAG CTGCTCAACA GACTAATGGT ATTTGGCAGT 
GCTGGCACAG TGGACTCTCT GTGCCACCTT AATGAATGGG GCCGCTGCTC CATCCCCATT 
CACGCCCTGC AGGCTGGTCT AAAGAACCAC CAGTTGGACA CAGTGGACTT CTTCCTGAAG 
AGCAAAAAGA ACATCCTGAC TCCACCCTCT GGGTATATTC TACCTGACCA ATCAGCTGCC 
ATCTCAACCC AGAGCCAGCT GAAGAATGTC CAGGATCTGT GTCCGGCTCT GGACCTTCTG 
CGTTGTGCCA TCAGAGACAA TCATACTGAG GCCCAGAGCC GACAATTTTC AGAACAGCTG 
CTCAGCATCA CCCTGGCCTT CCTCAACTCA CAAGTCAGGG CCGTCCTCTC TGCCACAGAG 
GAGCTGGATG AGAACTTGCA GGAGTGTGTG GAGATCTTGG ATAGTTACGT CTCGGATCTG 
CGCACCTTCA TGAAGAGGTT TCCCTGGCCA ACAGGTGGTG ACACTTCCAC CCTTGTCCCA 
TCCCCCATAC CTGTACCTGA GGAGGTGGAG AATGATGACT GGAGTTCCCT CTCTTCTGAG 
GAGGTGGTCG AGCGGGCAGT GCTGACCAAG CAGATTCCCC GGGCCCAGGC CTACCTGAGG 
AGCCAGAACA GGCCAGATCA GCGGCTGGAG GAGTTGAGGA GTACGGGGCT GCGTCTGGCC 
TTCAACTGCC TCACACACAG AGACCTGGAG CAGGCCACTG CACTGCTCAG GAACATGGGT 
TTCAACGTAA AGAAGCAACT GTATAACATA TGCCTCTACA CAGATGACCG GGACCTAAGG 
GACTTTGTGG TGGAGGAGTT GTCAAAGCAG AGCTATTTGT CTGATGAAGA GACACAGAGG 
GTGGAATTCA TCAAACAGAT TGAGGCTTTG TGTGCGGTGC CTGAGACGCG CTGCACAGAC 
CCTGTGGGCA GACAACGGGT TCCTCAGATG GCCCAGACAG ACCCAGAGTC TAAGAGCCTG 
CTGGAGGAGC TGGTAGACCA GAGAGGCTCC CAGTCAGAAC AGACACTGTG GAGTTCGCTG 
CGTCTGGACT GGGTCAGACA TTGGGACCAC AACACCCAGA GAGCCATCCT GCTGTCACGC 
CTACAAGACT CGGCAGTTGT CAGTTCCTGT GACCCGGGGG TCCTGTGGTC ATACCTCACC 
TCCCACCACG ACCAGCGGAG GGTAACCGAG TGGATTGAGA GCCTGGCCGC CCAGAACGGT 
GACCCTTGCA GCGCCCCCCA GTGGCCCGCC CTCACGGCAG AGGTGGTCAA CAACAACACC 
CGGTGTAGCG CCCACATGAG GAACCACATC CTCTACATGT TGGCCAGGAG AGGAGTGTTC 
ATCCAGGCCG AGATGGCTGA TTTTGAGCAT CTGTTGTGGA GGCTGGGCCA GGCAGGTGGG 
GTGATGCAGG CCAGCCCACC CATGCCCCAG TACCGCTCCC CTCAAGGACA CGACTTACAC 
ACACGCTTCA TCCACCACTG CCTGGACAAC GGCCTTCGCT ACCTGCTCTA CACCTACCTG 
GAGCACAACA GATTGACCCC CAGGAATTGC CCTGTCCTTG CTGATAATGG TCTATATGAA 
AGTCACCCGT GGTTTGAAAT GCTGGTGAAA ATGCAAGAGA TTACCAGAGA CCTCTCAGAT 
CCAGAACGCG TGTTCCAGGC CAGTCTGACC AGTGCCCAGG TCCTCATCCC AGGCAGCCAG 
GCCATGGTTA GCAGCATGCT CCTGGAAGGA CACAGTCTTC TGGCCCTCGC TACCATCATG 
TTTGCCCCGG GTGGCATCGA CCAGGTGGTG AGTCAGGGGG AGAGGGCTAG GAGCTCTGTG 
TCTAGGGTGG ACCCCCAGCT ACTGAAGATG GCCCTGAGCC CCTACCCCAA GCTCAAGGCA 
GCGCTCTTCC CTGTGAGCGG ACCACGAGGG ACCACCGCCA CCTCATCCGA TATCTCCGTC 
TACCACCTCA TGCAGTCTTT ACATCCATTG GATCCATCTC GACTGTTCGG GTGGCAGTCT 
GCCAATACAC TAGGCTCCAC CGAGACCTCA GTGGAGCTGC CCCATTTCTC TAGTCCTCAC 
CTCGTCAGTA AATACGCCCT CATTGAGAAC CTCGACTTCC TGTACTATCT GCGTCACGGA 
CGACCATCGT TTGCCTACGG AACGTTTCTC GTCCAACAGC TAGCCAACTC CAACGAAGTG 
AAGTCGCAGC TCAGTCATGC GGCGGAGCAG GCCTACAGCC TGGCCATGCT GTATTTCAGC 
GTTCCCTCTG TGGCAGCTGC ATGTGTGTGT TTCTGCGAGC TCCTGGGTGT ATGTAGCCTA 
AAGTTGCGGG TGGACCTCAA AGCCCTCAGT CTGATCCTCC GACTCCGGAG CCAGAGGCCT 
GGAGGGACCA GCACATTCTC CCTTAAAGAC TTACTGGCTC CTAAAGGCAG TAAGCTGGTG 
GAGGTGGAGA GGGAGACAGC GGAGGAGCTG CTCTTTGACT TGGAGGCAGC TGTGAAGGAC 
AGTTTGGAGA GGAAGGGCAT CAGCAGGTCA TCCTATGAGG CAGGTCAGGA GTGGGCTCTG 
CCCATACAGT TTTGCCAGCT ACATGCCCTG CCCCTGAGCA GCGTGTACCC ACACGACTGT 
GCAGCCGACA GCCAGTGGCT ACACTTCCTG CTCTTTGTCC AGCTGCACAG CTACCCCCCT 
CAGCAGGTGA GGTCCCTGGC GACCCATTTC AGCCCAGCCC TGCAGGCCCA CCTGACCCTG 
GCCTTCCAGA ACCTGCAGCT CAGCTCTCAG TGGAGGGTGG GGGAGGTGGA AGACAGGGAG 
GCCCAGGGTC AAGTCACCCT GCCCAGCGAG GAAGCCTCCG ACCCCCCCAA GGAGCTGTTC 
CAGGTGCTGC TGCAGAGCCA GGAGCAGGCC AGCCCCTGGA GACACCTCCT GGGGGAGGCC 
CTGGCACAGC ACTGCCCCAC GCTCACCACA CTAGCTGCCT GTCATCAGGA AGCCGACCTG 
CTCCAGTGCC TCTGCGTCTG GGTCCTGACC TCTGTGGATG AGGTCATTGC CACAGAGGCT 
ACCGCCCACC TGGAGGAGAG CCCGCGACGT CATGAGTGGA ACCTCCACGA TCTGTCAATC 
ATCTGGAAGA CACTGCTGAG GAAATGCAGG GTCAGGCCTC TCATACGAGG CTTCCAGCTC 
TTCCAGAGGG ACTCTCCCCT GATCCATGTG CTGCTCATGT ACGAGCTGTG CTACGACTAC 
AAAAACTACC CTGAAGCCAA GAAGAAACTC ATTGAGTTCC AGAAATGCCT GATCACGCTG 
AGGAGCAGTG GTGCTCATGC GCCAGCGGGC ATCCCTCTCC AGTGGGTGGA GTGCCAGGCC 
TCAGTCCTCC TGTTGACCAT GATGCAACAG TGCTCCACAC AATACGAGCT GCGCAGACTA 
CTGCAGCTGC TCGCAGACAT GGAGCGCCTG CTCAAGTCTA ACGGCCCGGA CTTCAAGAAG 
CTCAGCTTCC TCAGCCAGGT TCTCCAGGAC ACCTCGGTGT CTCTCTCTCC CAGGCTGCTT 
GAGAGCTATT CCCCTGAAAT CCTGCAGGGA GAGTGCCAGA GCATGTTGGA GAAGCTGCAA 
GACAGCGGCC TGTTCCACAA GGCCCGCCAG GTAGCTGAGC TGGCAGGGCT GCCCATTGAC 
AGCCTGGTCA TCAACGAGTT ACTCCAAGAC CTCAGTGCCC TGAAGGCCAA GCGGCACTGG 
GAGAGGAGGG AGACCCGGGT GTCCTTCTGG AGGAAATGCC ACGAGCAGTT CAGGACTAAC 
AGCACTGGCC CTGGGGCCGC CTCAGAGTTC TTCCTGGCCC AGGCAGGTGC CTCTGAGCCT 
CACCGGGGCT CCGTGGCCGA GACAGAGCTG CTGTGTGTCC AGGAGAGGTG CCTTCTCCTC 
AGCCTGGCCG GCCACTGGCT GTCCTGGCTC ACCCTAGCTC CCCTGGCACG TCTCGAGGAC 
CTGGAGAAGC AGCTGTGGAC AAGCCGAGTG CGCCAGCAAG TCCTGCTCAC CGCCATGGAG 
AAGGAGAGCA TGTTCGCCCT CCCGGCGCCC ACCATAGCCC CCGAGGAAAA CTCCTATGAG 
GCCCTGATCA GGGAGTTCTC CTTCTCCAAG ATGGCAGTGC TCAACGAGTC CAGGTACCTG 
AGCTTAGACG GCCTGCCCAG CTTCTCCCCT GAGGAAGAGG GTGGGGAGAA GACTGGGTCG 
GAGGAGCAGG GTGCTCTGGC TATCCTGGTG GGGCAGCTGT TGGACGAAGG GTGTATCCAC 
GAAGCGAGCC GTGTGTGCCG TTACTTCTCC TTCTGCTCCC GGGACATGTG GGTAGTGCTG 
CACTGCCGAG GCCTGGCCTC AGGCGAACTC CAGGCTGGCC TGCAGGACAT GCTCACCTTT 
GAGGGCCTGC CCAAGAAGAG CATTCCCAGC TCAACTAGTT TTAGCAGCCT GTCCTCCTTT 
GTGATGGTGC CCTCTCCAGA GGATCAAACG GTGACTCAGC TCCAGATACT GGTGGACGAA 
TGTCGCCACG GAAAGAGCTA CTGCAAGCAG GTCCTGAGTC TCTACGAGCT CTCCAAGGAG 
CTGCAGTGTT CATACAGTGA GATCTCAGCC GAGGAGCCTC AGGCAGTGCT GTGTAAGGTG 
CTACTGTCCC AGCAGCCGGA GCGCTATAAG AAGGCCCAGG CCTTCATCAG TGTGCAGGGC 
CTGCAGCCTG ACACCGTGGC CCAGCTTGTA TCCACTGCCG TGGTCCAGGG CCTGTTGGCC 
TCTGCACAGG ACGGAGAGAC TGAGAAGCAG ATGTTCAGTC CAGAGGAAGG GAGAGAGTCC 
TTCTTGCAGC TGGCCAAGCT GTGTGGGGAC CCCAACCTGG TGGGCATCAA ACTGCTGGAC 
CACATCCCCA CTGTGCCACT GGGAGAGCTC GGCTGCACGG TGGAGCTGTT GATCCTGGCC 
CACGATTGCT TCAGTCTGAC CTGTAACATG GAGGGCATAG TGAGGGTGCT GCAGGCTGCC 
CGCCACCTTA GCCACGCACA CCTGGCCCAC AGCGAACACT ACGGCCTGCT GGTGCGTCTC 
CTCACTGGCA TCGGCAGATA CAATGACATG ACCTACATCT TCGACCTGCT CAACCAGAAC 
CACCGCTTCG AGATGCTGCT CAGGAAAAAA GTGGAGTCGA ACTTCCGGCT GAAGACGGCC 
CTGCTGGACT ACATCAAGCG GTGTTTGCCG GGGGACAGTG AGAAGTACAA CATGGTGGCC 
CTATGCTTCA GCATGTGCAG AGAGATAGGA GAGAACCACG AGGGAGCCGC CCGCACCCAG 
CTCAAACTGA TCGAGTCCCA GCCTTGGGCA GTGACCCCTG AACTCAAAAG TGCCTTGATA 
AAGGTTCTTA CTCTCCTGAA GGATGCTGCA GAGAGCTATT CTAAGGACTC GTGTGTACGC 
CAGGCAGCAC GTTGTGTCAA AATGGCAAAG CTGACCACAC TCCAGCTCCA CTTCCTGAAC 
CACGGTCAAG ACCAACGTGT CATAAACCTG CGCCAATCAG ATCTCCTTGG TGCTATAGTG 
GCACTGCCCC GCTGTTACCA GGCTTTTGTG TTGTCGGAGG CCTACGACTA CAGTCCAGAC 
TGGGCGGAGG TGCTCTACCA GAAGGTCATC CTCAGTGGAG ACTTTGCTTA CCTAGAGGAG 
TTCCGACTTC ACAGGCCACT CCCCGCCTGC CTGTTTGAGG ACATATCACA AAAATTGACT 
CACAACACGC CTCCGAGCAG TGCGGGCCAG AACCTGAAGA GACTATTGCA ACACTGTGAT 
GACGTTTACA CCTACTACAA GCTTGCGTAT GAACACAAGT TCTTCGACGT GGCCAACATG 
TTACTTCAGG ACTCCAAAAC AAGCAGTTAC CTAAATGACA GGCTAGGTAC TAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013979447.1
CDS	220..7476
Translation	MIMINMLHRSETRDNCALEVALIPESNLCVNVVDTAKAKMAQGCSLLCCLSCAGGLTVGDISESKAPGRTVDGTYTDFAWEEVTVYNGARGSPRLLAVSSEFELTLYELEAEGPTSGPLVSASEFTADKLLQLVKEKALSVTEVVSLRLLSFVDARSFLLLNSSVVVQLQWQQGAEPETLSCCSIVLPPKAPLDTCTDYQLCRGILFVLTTAGVIYVFDSAEGRKLASVDLPSYHTAGLGEDEPAPYLDSFCLLQVSPDLGTAVAATQSNTAFAVDLNHYFRMYPEHLMCRVVPSRPPLRPQGPCDEDSLSISTYSHTVLGTSFSTDRSWEARLTSLYNKAKATPPSSPQTPGAPWFKDFPHIECGRAAIAAKLSQSRVPSGGATMQFSVPDTTAPVLLSVSEFSTVLTFVSPGNSETTVAFWDLQSQSVTYHRPTLPTVPIQRTAEEQLSLLLKTSGLSLVLFAVSQEELLNRLMVFGSAGTVDSLCHLNEWGRCSIPIHALQAGLKNHQLDTVDFFLKSKKNILTPPSGYILPDQSAAISTQSQLKNVQDLCPALDLLRCAIRDNHTEAQSRQFSEQLLSITLAFLNSQVRAVLSATEELDENLQECVEILDSYVSDLRTFMKRFPWPTGGDTSTLVPSPIPVPEEVENDDWSSLSSEEVVERAVLTKQIPRAQAYLRSQNRPDQRLEELRSTGLRLAFNCLTHRDLEQATALLRNMGFNVKKQLYNICLYTDDRDLRDFVVEELSKQSYLSDEETQRVEFIKQIEALCAVPETRCTDPVGRQRVPQMAQTDPESKSLLEELVDQRGSQSEQTLWSSLRLDWVRHWDHNTQRAILLSRLQDSAVVSSCDPGVLWSYLTSHHDQRRVTEWIESLAAQNGDPCSAPQWPALTAEVVNNNTRCSAHMRNHILYMLARRGVFIQAEMADFEHLLWRLGQAGGVMQASPPMPQYRSPQGHDLHTRFIHHCLDNGLRYLLYTYLEHNRLTPRNCPVLADNGLYESHPWFEMLVKMQEITRDLSDPERVFQASLTSAQVLIPGSQAMVSSMLLEGHSLLALATIMFAPGGIDQVVSQGERARSSVSRVDPQLLKMALSPYPKLKAALFPVSGPRGTTATSSDISVYHLMQSLHPLDPSRLFGWQSANTLGSTETSVELPHFSSPHLVSKYALIENLDFLYYLRHGRPSFAYGTFLVQQLANSNEVKSQLSHAAEQAYSLAMLYFSVPSVAAACVCFCELLGVCSLKLRVDLKALSLILRLRSQRPGGTSTFSLKDLLAPKGSKLVEVERETAEELLFDLEAAVKDSLERKGISRSSYEAGQEWALPIQFCQLHALPLSSVYPHDCAADSQWLHFLLFVQLHSYPPQQVRSLATHFSPALQAHLTLAFQNLQLSSQWRVGEVEDREAQGQVTLPSEEASDPPKELFQVLLQSQEQASPWRHLLGEALAQHCPTLTTLAACHQEADLLQCLCVWVLTSVDEVIATEATAHLEESPRRHEWNLHDLSIIWKTLLRKCRVRPLIRGFQLFQRDSPLIHVLLMYELCYDYKNYPEAKKKLIEFQKCLITLRSSGAHAPAGIPLQWVECQASVLLLTMMQQCSTQYELRRLLQLLADMERLLKSNGPDFKKLSFLSQVLQDTSVSLSPRLLESYSPEILQGECQSMLEKLQDSGLFHKARQVAELAGLPIDSLVINELLQDLSALKAKRHWERRETRVSFWRKCHEQFRTNSTGPGAASEFFLAQAGASEPHRGSVAETELLCVQERCLLLSLAGHWLSWLTLAPLARLEDLEKQLWTSRVRQQVLLTAMEKESMFALPAPTIAPEENSYEALIREFSFSKMAVLNESRYLSLDGLPSFSPEEEGGEKTGSEEQGALAILVGQLLDEGCIHEASRVCRYFSFCSRDMWVVLHCRGLASGELQAGLQDMLTFEGLPKKSIPSSTSFSSLSSFVMVPSPEDQTVTQLQILVDECRHGKSYCKQVLSLYELSKELQCSYSEISAEEPQAVLCKVLLSQQPERYKKAQAFISVQGLQPDTVAQLVSTAVVQGLLASAQDGETEKQMFSPEEGRESFLQLAKLCGDPNLVGIKLLDHIPTVPLGELGCTVELLILAHDCFSLTCNMEGIVRVLQAARHLSHAHLAHSEHYGLLVRLLTGIGRYNDMTYIFDLLNQNHRFEMLLRKKVESNFRLKTALLDYIKRCLPGDSEKYNMVALCFSMCREIGENHEGAARTQLKLIESQPWAVTPELKSALIKVLTLLKDAAESYSKDSCVRQAARCVKMAKLTTLQLHFLNHGQDQRVINLRQSDLLGAIVALPRCYQAFVLSEAYDYSPDWAEVLYQKVILSGDFAYLEEFRLHRPLPACLFEDISQKLTHNTPPSSAGQNLKRLLQHCDDVYTYYKLAYEHKFFDVANMLLQDSKTSSYLNDRLGTR

Target ORF information:

RefSeq Version	XM_014123972.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar spastic paraplegia 11 (autosomal recessive) (spg11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014123972.1

ORF Insert Sequence:

ATGATTATGA TAAATATGTT GCACAGATCT GAAACTCGAG ATAACTGTGC ATTAGAAGTC 
GCTCTCATCC CGGAGAGCAA CCTCTGTGTA AATGTTGTGG ACACAGCAAA AGCCAAAATG 
GCCCAAGGCT GCTCCTTGCT CTGCTGCTTG AGTTGTGCTG GGGGGCTGAC AGTCGGCGAC 
ATTTCAGAGA GCAAGGCACC GGGGAGGACG GTGGATGGCA CATATACAGA CTTTGCCTGG 
GAGGAGGTGA CAGTTTACAA TGGAGCCCGT GGCAGCCCTA GACTTCTGGC TGTGAGCTCT 
GAGTTTGAGC TGACACTGTA TGAACTGGAA GCAGAGGGCC CTACCTCTGG CCCTCTTGTC 
TCTGCCTCTG AGTTCACAGC AGATAAGCTA CTGCAGCTGG TCAAGGAGAA AGCCCTGAGT 
GTGACTGAGG TGGTGTCCTT GAGGCTGCTG TCCTTTGTGG ATGCCAGAAG CTTTCTCCTG 
CTCAACTCCA GTGTTGTGGT GCAGCTGCAG TGGCAGCAGG GGGCTGAACC GGAGACCCTG 
TCCTGCTGCA GCATCGTTCT GCCCCCAAAA GCCCCCCTGG ACACATGCAC TGACTACCAG 
CTCTGCAGAG GCATACTCTT TGTGTTGACC ACAGCTGGAG TCATATATGT GTTTGACAGT 
GCTGAGGGGA GGAAGCTGGC CAGTGTAGAC CTGCCCTCGT ACCACACTGC TGGGCTGGGG 
GAAGACGAGC CGGCCCCCTA CCTGGACTCC TTCTGTCTCC TCCAGGTGTC TCCTGACCTG 
GGTACTGCTG TTGCTGCCAC TCAGTCTAAC ACTGCCTTTG CTGTCGACCT CAACCACTAC 
TTCAGGATGT ACCCAGAGCA TCTGATGTGT CGGGTGGTCC CCAGTCGCCC TCCCCTCAGG 
CCCCAGGGTC CCTGTGACGA GGACAGCCTC TCCATCTCCA CCTACAGCCA CACTGTGCTG 
GGCACCTCCT TCAGCACTGA CCGGTCCTGG GAAGCTCGGC TGACTTCTCT GTACAACAAG 
GCCAAGGCTA CCCCTCCCTC TTCTCCTCAG ACTCCGGGGG CTCCTTGGTT TAAGGATTTC 
CCCCACATCG AGTGTGGGCG GGCAGCTATC GCTGCCAAGC TCTCCCAGAG CAGAGTCCCA 
TCAGGGGGAG CCACCATGCA GTTCTCTGTG CCCGACACAA CTGCCCCAGT GCTGCTCTCT 
GTCTCTGAAT TCTCCACTGT GTTGACCTTT GTCTCCCCTG GCAACAGCGA GACAACTGTG 
GCCTTCTGGG ATCTGCAGTC CCAGAGTGTT ACCTACCACC GGCCCACCTT GCCCACCGTG 
CCTATCCAGC GTACCGCAGA GGAGCAGCTC AGCCTGCTTC TGAAGACGTC AGGCCTGTCC 
CTGGTGCTGT TTGCTGTGTC ACAGGAGGAG CTGCTCAACA GACTAATGGT ATTTGGCAGT 
GCTGGCACAG TGGACTCTCT GTGCCACCTT AATGAATGGG GCCGCTGCTC CATCCCCATT 
CACGCCCTGC AGGCTGGTCT AAAGAACCAC CAGTTGGACA CAGTGGACTT CTTCCTGAAG 
AGCAAAAAGA ACATCCTGAC TCCACCCTCT GGGTATATTC TACCTGACCA ATCAGCTGCC 
ATCTCAACCC AGAGCCAGCT GAAGAATGTC CAGGATCTGT GTCCGGCTCT GGACCTTCTG 
CGTTGTGCCA TCAGAGACAA TCATACTGAG GCCCAGAGCC GACAATTTTC AGAACAGCTG 
CTCAGCATCA CCCTGGCCTT CCTCAACTCA CAAGTCAGGG CCGTCCTCTC TGCCACAGAG 
GAGCTGGATG AGAACTTGCA GGAGTGTGTG GAGATCTTGG ATAGTTACGT CTCGGATCTG 
CGCACCTTCA TGAAGAGGTT TCCCTGGCCA ACAGGTGGTG ACACTTCCAC CCTTGTCCCA 
TCCCCCATAC CTGTACCTGA GGAGGTGGAG AATGATGACT GGAGTTCCCT CTCTTCTGAG 
GAGGTGGTCG AGCGGGCAGT GCTGACCAAG CAGATTCCCC GGGCCCAGGC CTACCTGAGG 
AGCCAGAACA GGCCAGATCA GCGGCTGGAG GAGTTGAGGA GTACGGGGCT GCGTCTGGCC 
TTCAACTGCC TCACACACAG AGACCTGGAG CAGGCCACTG CACTGCTCAG GAACATGGGT 
TTCAACGTAA AGAAGCAACT GTATAACATA TGCCTCTACA CAGATGACCG GGACCTAAGG 
GACTTTGTGG TGGAGGAGTT GTCAAAGCAG AGCTATTTGT CTGATGAAGA GACACAGAGG 
GTGGAATTCA TCAAACAGAT TGAGGCTTTG TGTGCGGTGC CTGAGACGCG CTGCACAGAC 
CCTGTGGGCA GACAACGGGT TCCTCAGATG GCCCAGACAG ACCCAGAGTC TAAGAGCCTG 
CTGGAGGAGC TGGTAGACCA GAGAGGCTCC CAGTCAGAAC AGACACTGTG GAGTTCGCTG 
CGTCTGGACT GGGTCAGACA TTGGGACCAC AACACCCAGA GAGCCATCCT GCTGTCACGC 
CTACAAGACT CGGCAGTTGT CAGTTCCTGT GACCCGGGGG TCCTGTGGTC ATACCTCACC 
TCCCACCACG ACCAGCGGAG GGTAACCGAG TGGATTGAGA GCCTGGCCGC CCAGAACGGT 
GACCCTTGCA GCGCCCCCCA GTGGCCCGCC CTCACGGCAG AGGTGGTCAA CAACAACACC 
CGGTGTAGCG CCCACATGAG GAACCACATC CTCTACATGT TGGCCAGGAG AGGAGTGTTC 
ATCCAGGCCG AGATGGCTGA TTTTGAGCAT CTGTTGTGGA GGCTGGGCCA GGCAGGTGGG 
GTGATGCAGG CCAGCCCACC CATGCCCCAG TACCGCTCCC CTCAAGGACA CGACTTACAC 
ACACGCTTCA TCCACCACTG CCTGGACAAC GGCCTTCGCT ACCTGCTCTA CACCTACCTG 
GAGCACAACA GATTGACCCC CAGGAATTGC CCTGTCCTTG CTGATAATGG TCTATATGAA 
AGTCACCCGT GGTTTGAAAT GCTGGTGAAA ATGCAAGAGA TTACCAGAGA CCTCTCAGAT 
CCAGAACGCG TGTTCCAGGC CAGTCTGACC AGTGCCCAGG TCCTCATCCC AGGCAGCCAG 
GCCATGGTTA GCAGCATGCT CCTGGAAGGA CACAGTCTTC TGGCCCTCGC TACCATCATG 
TTTGCCCCGG GTGGCATCGA CCAGGTGGTG AGTCAGGGGG AGAGGGCTAG GAGCTCTGTG 
TCTAGGGTGG ACCCCCAGCT ACTGAAGATG GCCCTGAGCC CCTACCCCAA GCTCAAGGCA 
GCGCTCTTCC CTGTGAGCGG ACCACGAGGG ACCACCGCCA CCTCATCCGA TATCTCCGTC 
TACCACCTCA TGCAGTCTTT ACATCCATTG GATCCATCTC GACTGTTCGG GTGGCAGTCT 
GCCAATACAC TAGGCTCCAC CGAGACCTCA GTGGAGCTGC CCCATTTCTC TAGTCCTCAC 
CTCGTCAGTA AATACGCCCT CATTGAGAAC CTCGACTTCC TGTACTATCT GCGTCACGGA 
CGACCATCGT TTGCCTACGG AACGTTTCTC GTCCAACAGC TAGCCAACTC CAACGAAGTG 
AAGTCGCAGC TCAGTCATGC GGCGGAGCAG GCCTACAGCC TGGCCATGCT GTATTTCAGC 
GTTCCCTCTG TGGCAGCTGC ATGTGTGTGT TTCTGCGAGC TCCTGGGTGT ATGTAGCCTA 
AAGTTGCGGG TGGACCTCAA AGCCCTCAGT CTGATCCTCC GACTCCGGAG CCAGAGGCCT 
GGAGGGACCA GCACATTCTC CCTTAAAGAC TTACTGGCTC CTAAAGGCAG TAAGCTGGTG 
GAGGTGGAGA GGGAGACAGC GGAGGAGCTG CTCTTTGACT TGGAGGCAGC TGTGAAGGAC 
AGTTTGGAGA GGAAGGGCAT CAGCAGGTCA TCCTATGAGG CAGGTCAGGA GTGGGCTCTG 
CCCATACAGT TTTGCCAGCT ACATGCCCTG CCCCTGAGCA GCGTGTACCC ACACGACTGT 
GCAGCCGACA GCCAGTGGCT ACACTTCCTG CTCTTTGTCC AGCTGCACAG CTACCCCCCT 
CAGCAGGTGA GGTCCCTGGC GACCCATTTC AGCCCAGCCC TGCAGGCCCA CCTGACCCTG 
GCCTTCCAGA ACCTGCAGCT CAGCTCTCAG TGGAGGGTGG GGGAGGTGGA AGACAGGGAG 
GCCCAGGGTC AAGTCACCCT GCCCAGCGAG GAAGCCTCCG ACCCCCCCAA GGAGCTGTTC 
CAGGTGCTGC TGCAGAGCCA GGAGCAGGCC AGCCCCTGGA GACACCTCCT GGGGGAGGCC 
CTGGCACAGC ACTGCCCCAC GCTCACCACA CTAGCTGCCT GTCATCAGGA AGCCGACCTG 
CTCCAGTGCC TCTGCGTCTG GGTCCTGACC TCTGTGGATG AGGTCATTGC CACAGAGGCT 
ACCGCCCACC TGGAGGAGAG CCCGCGACGT CATGAGTGGA ACCTCCACGA TCTGTCAATC 
ATCTGGAAGA CACTGCTGAG GAAATGCAGG GTCAGGCCTC TCATACGAGG CTTCCAGCTC 
TTCCAGAGGG ACTCTCCCCT GATCCATGTG CTGCTCATGT ACGAGCTGTG CTACGACTAC 
AAAAACTACC CTGAAGCCAA GAAGAAACTC ATTGAGTTCC AGAAATGCCT GATCACGCTG 
AGGAGCAGTG GTGCTCATGC GCCAGCGGGC ATCCCTCTCC AGTGGGTGGA GTGCCAGGCC 
TCAGTCCTCC TGTTGACCAT GATGCAACAG TGCTCCACAC AATACGAGCT GCGCAGACTA 
CTGCAGCTGC TCGCAGACAT GGAGCGCCTG CTCAAGTCTA ACGGCCCGGA CTTCAAGAAG 
CTCAGCTTCC TCAGCCAGGT TCTCCAGGAC ACCTCGGTGT CTCTCTCTCC CAGGCTGCTT 
GAGAGCTATT CCCCTGAAAT CCTGCAGGGA GAGTGCCAGA GCATGTTGGA GAAGCTGCAA 
GACAGCGGCC TGTTCCACAA GGCCCGCCAG GTAGCTGAGC TGGCAGGGCT GCCCATTGAC 
AGCCTGGTCA TCAACGAGTT ACTCCAAGAC CTCAGTGCCC TGAAGGCCAA GCGGCACTGG 
GAGAGGAGGG AGACCCGGGT GTCCTTCTGG AGGAAATGCC ACGAGCAGTT CAGGACTAAC 
AGCACTGGCC CTGGGGCCGC CTCAGAGTTC TTCCTGGCCC AGGCAGGTGC CTCTGAGCCT 
CACCGGGGCT CCGTGGCCGA GACAGAGCTG CTGTGTGTCC AGGAGAGGTG CCTTCTCCTC 
AGCCTGGCCG GCCACTGGCT GTCCTGGCTC ACCCTAGCTC CCCTGGCACG TCTCGAGGAC 
CTGGAGAAGC AGCTGTGGAC AAGCCGAGTG CGCCAGCAAG TCCTGCTCAC CGCCATGGAG 
AAGGAGAGCA TGTTCGCCCT CCCGGCGCCC ACCATAGCCC CCGAGGAAAA CTCCTATGAG 
GCCCTGATCA GGGAGTTCTC CTTCTCCAAG ATGGCAGTGC TCAACGAGTC CAGGTACCTG 
AGCTTAGACG GCCTGCCCAG CTTCTCCCCT GAGGAAGAGG GTGGGGAGAA GACTGGGTCG 
GAGGAGCAGG GTGCTCTGGC TATCCTGGTG GGGCAGCTGT TGGACGAAGG GTGTATCCAC 
GAAGCGAGCC GTGTGTGCCG TTACTTCTCC TTCTGCTCCC GGGACATGTG GGTAGTGCTG 
CACTGCCGAG GCCTGGCCTC AGGCGAACTC CAGGCTGGCC TGCAGGACAT GCTCACCTTT 
GAGGGCCTGC CCAAGAAGAG CATTCCCAGC TCAACTAGTT TTAGCAGCCT GTCCTCCTTT 
GTGATGGTGC CCTCTCCAGA GGATCAAACG GTGACTCAGC TCCAGATACT GGTGGACGAA 
TGTCGCCACG GAAAGAGCTA CTGCAAGCAG GTCCTGAGTC TCTACGAGCT CTCCAAGGAG 
CTGCAGTGTT CATACAGTGA GATCTCAGCC GAGGAGCCTC AGGCAGTGCT GTGTAAGGTG 
CTACTGTCCC AGCAGCCGGA GCGCTATAAG AAGGCCCAGG CCTTCATCAG TGTGCAGGGC 
CTGCAGCCTG ACACCGTGGC CCAGCTTGTA TCCACTGCCG TGGTCCAGGG CCTGTTGGCC 
TCTGCACAGG ACGGAGAGAC TGAGAAGCAG ATGTTCAGTC CAGAGGAAGG GAGAGAGTCC 
TTCTTGCAGC TGGCCAAGCT GTGTGGGGAC CCCAACCTGG TGGGCATCAA ACTGCTGGAC 
CACATCCCCA CTGTGCCACT GGGAGAGCTC GGCTGCACGG TGGAGCTGTT GATCCTGGCC 
CACGATTGCT TCAGTCTGAC CTGTAACATG GAGGGCATAG TGAGGGTGCT GCAGGCTGCC 
CGCCACCTTA GCCACGCACA CCTGGCCCAC AGCGAACACT ACGGCCTGCT GGTGCGTCTC 
CTCACTGGCA TCGGCAGATA CAATGACATG ACCTACATCT TCGACCTGCT CAACCAGAAC 
CACCGCTTCG AGATGCTGCT CAGGAAAAAA GTGGAGTCGA ACTTCCGGCT GAAGACGGCC 
CTGCTGGACT ACATCAAGCG GTGTTTGCCG GGGGACAGTG AGAAGTACAA CATGGTGGCC 
CTATGCTTCA GCATGTGCAG AGAGATAGGA GAGAACCACG AGGGAGCCGC CCGCACCCAG 
CTCAAACTGA TCGAGTCCCA GCCTTGGGCA GTGACCCCTG AACTCAAAAG TGCCTTGATA 
AAGGTTCTTA CTCTCCTGAA GGATGCTGCA GAGAGCTATT CTAAGGACTC GTGTGTACGC 
CAGGCAGCAC GTTGTGTCAA AATGGCAAAG CTGACCACAC TCCAGCTCCA CTTCCTGAAC 
CACGGTCAAG ACCAACGTGT CATAAACCTG CGCCAATCAG ATCTCCTTGG TGCTATAGTG 
GCACTGCCCC GCTGTTACCA GGCTTTTGTG TTGTCGGAGG CCTACGACTA CAGTCCAGAC 
TGGGCGGAGG TGCTCTACCA GAAGGTCATC CTCAGTGGAG ACTTTGCTTA CCTAGAGGAG 
TTCCGACTTC ACAGGCCACT CCCCGCCTGC CTGTTTGAGG ACATATCACA AAAATTGACT 
CACAACACGC CTCCGAGCAG TGCGGGCCAG AACCTGAAGA GACTATTGCA ACACTGTGAT 
GACGTTTACA CCTACTACAA GCTTGCGTAT GAACACAAGT TCTTCGACGT GGCCAACATG 
TTACTTCAGG ACTCCAAAAC AAGCAGTTAC CTAAATGACA GGCTAGGTAC TAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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spg11 cDNA ORF clone, Salmo salar(Atlantic salmon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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