TJP1 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	TJP1
Entrez Gene ID	103245955
Full Name	tight junction protein 1
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008017222.1	XP_008015413.1	tight junction protein ZO-1 isoform X2
XM_008017225.1	XP_008015416.1	tight junction protein ZO-1 isoform X5
XM_008017226.1	XP_008015417.1	tight junction protein ZO-1 isoform X6
XM_008017228.1	XP_008015419.1	tight junction protein ZO-1 isoform X7
XM_008017221.1	XP_008015412.1	tight junction protein ZO-1 isoform X1
XM_008017223.1	XP_008015414.1	tight junction protein ZO-1 isoform X3
XM_008017224.1	XP_008015415.1	tight junction protein ZO-1 isoform X4

Pathways from BioSystems
Gene Ontology

The following TJP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCh123103	XM_008017221.1 Latest version!	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh123104	XM_008017222.1 Latest version!	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh123105	XM_008017223.1 Latest version!	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh123106	XM_008017224.1 Latest version!	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh123107	XM_008017225.1 Latest version!	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh123108	XM_008017226.1 Latest version!	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh123109	XM_008017228.1 Latest version!	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TJP1 ( XM_008017221.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008015412.1 Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCh123103
Clone ID Related Accession (Same CDS sequence)	XM_008017221.1
Accession Version	XM_008017221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735276.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGCATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TCGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTGGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCTCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CCACCAAAGT CACACTGGTT AAATCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGAAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTGTTGAAC GTCCCTGATC TGTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCCTC AGATCATTCT 
GGTCGATCAC ACGATAAGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCAGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAGTTTC TAAGCCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CAGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGACCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCTTGCGACT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTGGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GCGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGGGGAGA CCGTGCTGAC 
TTCTGGAGAT TCCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCTGCTC AGCCTGTTCA AACAAAGTTT CCGGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTT TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCTAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAATCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGATTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCACTGAA AGAAGCAATT CAACAACAGC AAAGCCAGCT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTA 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCACTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTACA GAAAGGATCC ATATCCCGAG GAAATGATGA GACAAAACCA TGTTTTGAAA 
CAGCCAGCCG TTGGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACGTACAGA 
TATGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCGTCTCGGC CACCTTTTGA TAATCAGCAC TCTCGAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGTGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCCTAT 
GACAGCAGAC CACGTTATGA ACAGGCACCT AGAGCGTCTG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC GCATGGTAGA CACAGGCCGG AAACCCAGCC CTACCCTTCA 
GCAGGGCCCA AGCCCGCGGA GCCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTCCCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAT 
ACCAAACCGC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGCCACT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTAT ACAGGATCCC AGAACCTCAA AAGCCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTCTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCTGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTA GATAGATCGT TTGGTGAGAA ACGCTATGAA 
CCCCTCCAGG TCACTCCCCC TCCTCCTCCA TTGCCCTCGC AATACGCCCA GCCATCTCAA 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTA TCCAAACCAG ACCCACCTCC GTCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACGGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCGTTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCGCATAAA 
CCTGACTTGT CAAAAACTCC CACTTCTCCA AAAACTCTTG TGAAATCGCA CAGTTTGGCA 
CAGCCTTCTG AGTTTGACAG TGGAGTTGAA ACTTTCTCTA TCCATGCAGA GAAGCCTAAA 
TATCAAATAA ATAATATCAG CACAGTGCCT AAAACTATTC CTGTGAGTCC TTCAGCTGTG 
GAAGAGGATG AAGATGAAGA TGGTCATACT GTGGTGGCCA CAGCGCGAGG CATATTTAAC 
AGCAATGGTG GCGTGCTGAG TTCCATAGAA ACTGGCGTCA GTATAATTAT CCCTCAAGGA 
GCCATTCCTG AAGGAGTTGA GCAGGAAATC TATTTCAAGG TCTGCCGAGA CAACAGCATC 
CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACACTGCTGA GTCCCTTGGT GATGTGTGGT 
CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTGCGCT TACCACACTG TGCGTCCATG 
ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTATC TCGTTGGAGC AAACTGTGTT 
TCTGTCCTTA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008015412.1
CDS	418..5721
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDKPPRRSRSRSPDQQSEPSDHSRHSPQQPSNGSLRSRDEERVSKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQSQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTTYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVGHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSRDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRHRPETQPYPSAGPKPAEPKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTATFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPLQVTPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSKTPTSPKTLVKSHSLAQPSEFDSGVETFSIHAEKPKYQINNISTVPKTIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_008017221.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008017221.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGCATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TCGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTGGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCTCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CCACCAAAGT CACACTGGTT AAATCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGAAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTGTTGAAC GTCCCTGATC TGTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCCTC AGATCATTCT 
GGTCGATCAC ACGATAAGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCAGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAGTTTC TAAGCCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CAGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGACCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCTTGCGACT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTGGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GCGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGGGGAGA CCGTGCTGAC 
TTCTGGAGAT TCCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCTGCTC AGCCTGTTCA AACAAAGTTT CCGGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTT TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCTAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAATCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGATTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCACTGAA AGAAGCAATT CAACAACAGC AAAGCCAGCT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTA 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCACTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTACA GAAAGGATCC ATATCCCGAG GAAATGATGA GACAAAACCA TGTTTTGAAA 
CAGCCAGCCG TTGGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACGTACAGA 
TATGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCGTCTCGGC CACCTTTTGA TAATCAGCAC TCTCGAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGTGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCCTAT 
GACAGCAGAC CACGTTATGA ACAGGCACCT AGAGCGTCTG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC GCATGGTAGA CACAGGCCGG AAACCCAGCC CTACCCTTCA 
GCAGGGCCCA AGCCCGCGGA GCCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTCCCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAT 
ACCAAACCGC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGCCACT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTAT ACAGGATCCC AGAACCTCAA AAGCCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTCTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCTGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTA GATAGATCGT TTGGTGAGAA ACGCTATGAA 
CCCCTCCAGG TCACTCCCCC TCCTCCTCCA TTGCCCTCGC AATACGCCCA GCCATCTCAA 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTA TCCAAACCAG ACCCACCTCC GTCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACGGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCGTTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCGCATAAA 
CCTGACTTGT CAAAAACTCC CACTTCTCCA AAAACTCTTG TGAAATCGCA CAGTTTGGCA 
CAGCCTTCTG AGTTTGACAG TGGAGTTGAA ACTTTCTCTA TCCATGCAGA GAAGCCTAAA 
TATCAAATAA ATAATATCAG CACAGTGCCT AAAACTATTC CTGTGAGTCC TTCAGCTGTG 
GAAGAGGATG AAGATGAAGA TGGTCATACT GTGGTGGCCA CAGCGCGAGG CATATTTAAC 
AGCAATGGTG GCGTGCTGAG TTCCATAGAA ACTGGCGTCA GTATAATTAT CCCTCAAGGA 
GCCATTCCTG AAGGAGTTGA GCAGGAAATC TATTTCAAGG TCTGCCGAGA CAACAGCATC 
CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACACTGCTGA GTCCCTTGGT GATGTGTGGT 
CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTGCGCT TACCACACTG TGCGTCCATG 
ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTATC TCGTTGGAGC AAACTGTGTT 
TCTGTCCTTA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_008017222.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008015413.1 Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

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CloneID	OCh123104
Clone ID Related Accession (Same CDS sequence)	XM_008017222.1
Accession Version	XM_008017222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735276.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTGTG ACTTATTTAA GAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
ACAGTGACGC TTCACAGGGC TCCTGGATTT GGATTTGGCA TTGCAATATC TGGTGGACGA 
GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATCGTGA TTTCAGATGT GCTGAAAGGA 
GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTGG CAATGGTTAA CGGAGTTTCA 
ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA 
AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA 
CCAGTATCTG ATAATGAAGA AGATAGTTAC GATGAGGAAA TACATGATCC AAGAAGTGGC 
CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT 
AGAGAGAGGA GCTTGTCTCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT 
AAACCCACCA AAGTCACACT GGTTAAATCC CGGAAGAATG AAGAATATGG TCTTCGATTG 
GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGAAAT 
ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG 
ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA 
GATGAACGGG CTACGCTGTT GAACGTCCCT GATCTGTCTG ACAGCATCCA CTCTGCTAAT 
GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CCTCAGATCA TTCTGGTCGA 
TCACACGATA AGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCA GTCAGAGCCT 
TCTGATCATT CCAGACACTC TCCACAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT 
GAAGAGAGAG TTTCTAAGCC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC 
ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT 
CCAGAACCAA AGCCTGTGTA TGCCCAGGTT GGGCAACCAG ATGTGGATTT ACCTGTCAGT 
CCATCAGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG ACCCAGCATG 
AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGCTTGC GACTGGCTGG TGGAAATGAT 
GTTGGAATAT TTGTAGCTGG CGTTCTGGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG 
GAAGGCGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA 
GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG 
AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA 
ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG 
TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT 
GGCAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG 
CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGGG GAGACCGTGC TGACTTCTGG 
AGATTCCGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCT 
GCTCAGCCTG TTCAAACAAA GTTTCCGGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA 
TTTTTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCT 
AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC 
CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA 
CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT 
CCAATTGTTG TATTTCTTAA TCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGA 
TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT 
AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG 
TATGGTGCAC TGAAAGAAGC AATTCAACAA CAGCAAAGCC AGCTGGTATG GGTTTCTGAG 
GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTATCCTAC 
CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC 
TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT 
AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA 
GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG 
CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA 
GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC 
TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT 
CCTTCCACCA CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT 
CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG 
TACAGAAAGG ATCCATATCC CGAGGAAATG ATGAGACAAA ACCATGTTTT GAAACAGCCA 
GCCGTTGGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA 
CTCCCATACG TGGAGAAACA AGCCAGCAGA GACCTTGAGC AGCCCACGTA CAGATATGAG 
TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT 
CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCGTCT 
CGGCCACCTT TTGATAATCA GCACTCTCGA GACCTTGACT CCAGACAGCA TCCCGAAGAG 
TCCTCAGAAC GTGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC CTATGACAGC 
AGACCACGTT ATGAACAGGC ACCTAGAGCG TCTGCCCTGC GGCACGAAGA GCAGCCAGCT 
CCTGGGTATG ACACGCATGG TAGACACAGG CCGGAAACCC AGCCCTACCC TTCAGCAGGG 
CCCAAGCCCG CGGAGCCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTC 
CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT 
GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAATACCAAA 
CCGCTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG 
TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG 
AAGGATGTAA ATGACACTGC CACTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT 
GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAAT TCAGTGAACA TGACAAAACT 
CTATACAGGA TCCCAGAACC TCAAAAGCCT CAACTGAAGC CACCTGAAGA TATCGTTCGG 
TCAAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT CTCATACTTT 
GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CTGCCAGCCA TCTCTCCGAG 
CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA 
AAGCCTCCTG AAGCTGATGG TGTAGATAGA TCGTTTGGTG AGAAACGCTA TGAACCCCTC 
CAGGTCACTC CCCCTCCTCC TCCATTGCCC TCGCAATACG CCCAGCCATC TCAACCTGTC 
ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA 
TTGGATTTTC AGAATTCCTT AGTATCCAAA CCAGACCCAC CTCCGTCTCA GAATAAGCCA 
GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA 
AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC GGTCACTCCA 
GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCGTT TGAACGCAAG 
TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCGCA TAAACCTGAC 
TTGTCAAAAA CTCCCACTTC TCCAAAAACT CTTGTGAAAT CGCACAGTTT GGCACAGCCT 
TCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCTATCCATG CAGAGAAGCC TAAATATCAA 
ATAAATAATA TCAGCACAGT GCCTAAAACT ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG 
GATGAAGATG AAGATGGTCA TACTGTGGTG GCCACAGCGC GAGGCATATT TAACAGCAAT 
GGTGGCGTGC TGAGTTCCAT AGAAACTGGC GTCAGTATAA TTATCCCTCA AGGAGCCATT 
CCTGAAGGAG TTGAGCAGGA AATCTATTTC AAGGTCTGCC GAGACAACAG CATCCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACACTG CTGAGTCCCT TGGTGATGTG TGGTCCCCAT 
GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC 
CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008015413.1
CDS	1..5298
Translation	MPCDLFKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDKPPRRSRSRSPDQQSEPSDHSRHSPQQPSNGSLRSRDEERVSKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQSQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTTYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVGHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSRDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRHRPETQPYPSAGPKPAEPKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTATFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPLQVTPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSKTPTSPKTLVKSHSLAQPSEFDSGVETFSIHAEKPKYQINNISTVPKTIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_008017222.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008017222.1

ORF Insert Sequence:

ATGCCGTGTG ACTTATTTAA GAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
ACAGTGACGC TTCACAGGGC TCCTGGATTT GGATTTGGCA TTGCAATATC TGGTGGACGA 
GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATCGTGA TTTCAGATGT GCTGAAAGGA 
GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTGG CAATGGTTAA CGGAGTTTCA 
ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA 
AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA 
CCAGTATCTG ATAATGAAGA AGATAGTTAC GATGAGGAAA TACATGATCC AAGAAGTGGC 
CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT 
AGAGAGAGGA GCTTGTCTCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT 
AAACCCACCA AAGTCACACT GGTTAAATCC CGGAAGAATG AAGAATATGG TCTTCGATTG 
GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGAAAT 
ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG 
ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA 
GATGAACGGG CTACGCTGTT GAACGTCCCT GATCTGTCTG ACAGCATCCA CTCTGCTAAT 
GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CCTCAGATCA TTCTGGTCGA 
TCACACGATA AGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCA GTCAGAGCCT 
TCTGATCATT CCAGACACTC TCCACAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT 
GAAGAGAGAG TTTCTAAGCC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC 
ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT 
CCAGAACCAA AGCCTGTGTA TGCCCAGGTT GGGCAACCAG ATGTGGATTT ACCTGTCAGT 
CCATCAGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG ACCCAGCATG 
AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGCTTGC GACTGGCTGG TGGAAATGAT 
GTTGGAATAT TTGTAGCTGG CGTTCTGGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG 
GAAGGCGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA 
GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG 
AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA 
ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG 
TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT 
GGCAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG 
CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGGG GAGACCGTGC TGACTTCTGG 
AGATTCCGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCT 
GCTCAGCCTG TTCAAACAAA GTTTCCGGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA 
TTTTTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCT 
AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC 
CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA 
CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT 
CCAATTGTTG TATTTCTTAA TCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGA 
TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATATG AGCGATCTCA TAAACTTCGT 
AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG 
TATGGTGCAC TGAAAGAAGC AATTCAACAA CAGCAAAGCC AGCTGGTATG GGTTTCTGAG 
GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTATCCTAC 
CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC 
TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT 
AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA 
GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG 
CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA 
GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC 
TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT 
CCTTCCACCA CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT 
CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG 
TACAGAAAGG ATCCATATCC CGAGGAAATG ATGAGACAAA ACCATGTTTT GAAACAGCCA 
GCCGTTGGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA 
CTCCCATACG TGGAGAAACA AGCCAGCAGA GACCTTGAGC AGCCCACGTA CAGATATGAG 
TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT 
CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCGTCT 
CGGCCACCTT TTGATAATCA GCACTCTCGA GACCTTGACT CCAGACAGCA TCCCGAAGAG 
TCCTCAGAAC GTGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC CTATGACAGC 
AGACCACGTT ATGAACAGGC ACCTAGAGCG TCTGCCCTGC GGCACGAAGA GCAGCCAGCT 
CCTGGGTATG ACACGCATGG TAGACACAGG CCGGAAACCC AGCCCTACCC TTCAGCAGGG 
CCCAAGCCCG CGGAGCCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTC 
CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT 
GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAATACCAAA 
CCGCTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG 
TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG 
AAGGATGTAA ATGACACTGC CACTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT 
GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAAT TCAGTGAACA TGACAAAACT 
CTATACAGGA TCCCAGAACC TCAAAAGCCT CAACTGAAGC CACCTGAAGA TATCGTTCGG 
TCAAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT CTCATACTTT 
GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CTGCCAGCCA TCTCTCCGAG 
CCTGCAAAGC CAGCTCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAGGGA 
AAGCCTCCTG AAGCTGATGG TGTAGATAGA TCGTTTGGTG AGAAACGCTA TGAACCCCTC 
CAGGTCACTC CCCCTCCTCC TCCATTGCCC TCGCAATACG CCCAGCCATC TCAACCTGTC 
ACCAGCGCGT CTCTCCACAT ACATTCTAAG GGAGCACATG GTGAAGGTAA TTCAGTGTCA 
TTGGATTTTC AGAATTCCTT AGTATCCAAA CCAGACCCAC CTCCGTCTCA GAATAAGCCA 
GCAACTTTCA GACCACCAAA CCGAGAAGAT ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA 
AGTTTTCCAG ATAAAGCCCC AGTTAATGGA ACTGAACAGA CTCAGAAAAC GGTCACTCCA 
GCATACAATC GATTCACACC AAAACCATAT ACAAGTTCTG CCCGACCGTT TGAACGCAAG 
TTTGAAAGTC CTAAATTCAA TCACAATCTT CTGCCAAGTG AAACTGCGCA TAAACCTGAC 
TTGTCAAAAA CTCCCACTTC TCCAAAAACT CTTGTGAAAT CGCACAGTTT GGCACAGCCT 
TCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCTATCCATG CAGAGAAGCC TAAATATCAA 
ATAAATAATA TCAGCACAGT GCCTAAAACT ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG 
GATGAAGATG AAGATGGTCA TACTGTGGTG GCCACAGCGC GAGGCATATT TAACAGCAAT 
GGTGGCGTGC TGAGTTCCAT AGAAACTGGC GTCAGTATAA TTATCCCTCA AGGAGCCATT 
CCTGAAGGAG TTGAGCAGGA AATCTATTTC AAGGTCTGCC GAGACAACAG CATCCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACACTG CTGAGTCCCT TGGTGATGTG TGGTCCCCAT 
GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC 
CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_008017223.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008015414.1 Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCh123105
Clone ID Related Accession (Same CDS sequence)	XM_008017223.1
Accession Version	XM_008017223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735276.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA CAGCTATATG GGAACAACAT ACAGTGACGC TTCACAGGGC TCCTGGATTT 
GGATTTGGCA TTGCAATATC TGGTGGACGA GATAATCCTC ATTTTCAGAG TGGGGAAACG 
TCAATCGTGA TTTCAGATGT GCTGAAAGGA GGACCAGCTG AAGGACAGCT ACAGGAAAAT 
GACCGAGTGG CAATGGTTAA CGGAGTTTCA ATGGATAATG TTGAACATGC TTTTGCTGTT 
CAGCAACTAA GGAAAAGTGG GAAAAATGCA AAAATTACAA TTAGAAGGAA GAAGAAAGTT 
CAAATACCAG TAAGTCGTCC TGATCCTGAA CCAGTATCTG ATAATGAAGA AGATAGTTAC 
GATGAGGAAA TACATGATCC AAGAAGTGGC CGGAGTGGTG TGGTTAACAG AAGGAGTGAG 
AAGATTTGGC CGAGGGATAG AAGTGCAAGT AGAGAGAGGA GCTTGTCTCC GCGGTCAGAC 
AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT AAACCCACCA AAGTCACACT GGTTAAATCC 
CGGAAGAATG AAGAATATGG TCTTCGATTG GCAAGCCATA TATTTGTTAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGAAAT ATTCAAGAAG GTGATGTTGT ATTGAAGATA 
AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AGACATTGAT AGAAAGGTCT 
AAAGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGGG CTACGCTGTT GAACGTCCCT 
GATCTGTCTG ACAGCATCCA CTCTGCTAAT GCCTCTGAGA GAGACGACAT TTCAGAAATT 
CAGTCACTGG CCTCAGATCA TTCTGGTCGA TCACACGATA AGCCTCCCCG CCGCAGCCGG 
TCACGATCTC CTGACCAGCA GTCAGAGCCT TCTGATCATT CCAGACACTC TCCACAGCAG 
CCAAGCAATG GCAGTCTCCG GAGTAGAGAT GAAGAGAGAG TTTCTAAGCC TGGGGCTGTC 
TCAACTCCTG TAAAGCATGC TGATGATCAC ACACCTAAAA CAGTGGAAGA AGTTACAGTT 
GAAAGAAATG AGAAACAAAC ACCTTCTCTT CCAGAACCAA AGCCTGTGTA TGCCCAGGTT 
GGGCAACCAG ATGTGGATTT ACCTGTCAGT CCATCAGATG GTGTCCTACC TAATTCAACT 
CATGAAGATG GGATTCTTCG ACCCAGCATG AAATTGGTAA AATTCAGAAA AGGAGATAGT 
GTGGGCTTGC GACTGGCTGG TGGAAATGAT GTTGGAATAT TTGTAGCTGG CGTTCTGGAA 
GATAGCCCTG CAGCCAAGGA AGGCTTAGAG GAAGGCGATC AAATTCTCAG GGTAAACAAC 
GTAGATTTTA CAAATATCAT AAGAGAAGAA GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA 
GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTATCGTCG CATTGTAGAA 
TCAGATGTAG GAGATTCTTT CTATATTAGA ACCCATTTTG AATATGAAAA GGAATCTCCC 
TATGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGTGTTG TGGATACCTT GTACAATGGA 
AAACTGGGCT CTTGGCTTGC TATTCGAATT GGCAAAAATC ATAAGGAGGT AGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAGCAG CTAGCCAGTG TACAGTATAC ACTTCCAAAA 
ACAGCAGGGG GAGACCGTGC TGACTTCTGG AGATTCCGAG GTCTTCGCAG CTCCAAGAGA 
AATCTTCGAA AAAGCAGAGA GGATTTGTCT GCTCAGCCTG TTCAAACAAA GTTTCCGGCT 
TATGAAAGAG TGGTTCTTCG AGAAGCTGGA TTTTTGAGGC CTGTAACCAT TTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGCTGGCT AGAGAAGAAC CAGATATTTA TCAAATTGCA 
AAGAGTGAAC CACGAGACGC TGGAACTGAC CAACGTAGCT CTGGCATTAT TCGCCTGCAT 
ACAATAAAGC AAATCATAGA TCAAGACAAA CATGCTTTAT TAGATGTAAC ACCAAATGCA 
GTTGATCGTC TTAACTATGC CCAGTGGTAT CCAATTGTTG TATTTCTTAA TCCTGATTCT 
AAGCAAGGAG TAAAAACAAT GAGAATGAGA TTATGTCCAG AATCTCGGAA AAGTGCCAGG 
AAGTTATATG AGCGATCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACTACA 
ATTAACTTAA ATTCAATGAA TGATGGTTGG TATGGTGCAC TGAAAGAAGC AATTCAACAA 
CAGCAAAGCC AGCTGGTATG GGTTTCTGAG GGAAAGGCGG ATGGTGCTAC AAGTGATGAC 
CTTGATTTGC ATGATGATCG TCTATCCTAC CTGTCAGCTC CAGGTAGTGA ATACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGACACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC ACCGGAGTCT 
GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGACTCCT CTGGAATGCA TCATGAAAAC 
CAAACATATC CTCCTTACTC ACCACAAGCG CAGCCACAAC CAATTCATAG AATAGACTCC 
CCTGGATTTA AGCCAGCCTC TCAACAGAAA GCAGAAGCTT CATCTCCAGT CCCTTACCTT 
TCGCCTGAAA CAAACCCAGC ATCATCAACC TCTGCTGTTA ATCATAATGT AAATTTAACT 
AATGTCAGAC TGGAGGAGCC CACCCCAGCT CCTTCCACCA CTTACTCACC ACAAGCTGAT 
TCTTTAAGAA CACCAAGTAC TGAGGCAGCT CACATAATGC TAAGAGATCA AGAACCATCA 
TTGTCGTCGC ATGTAGATCC AACAAAGGTG TACAGAAAGG ATCCATATCC CGAGGAAATG 
ATGAGACAAA ACCATGTTTT GAAACAGCCA GCCGTTGGTC ACCCAGGGCA CAGGCCAGAC 
AAAGAGCCTA ATCTGACCTA TGAACCCCAA CTCCCATACG TGGAGAAACA AGCCAGCAGA 
GACCTTGAGC AGCCCACGTA CAGATATGAG TCCTCAAGCT ATACGGACCA GTTTTCTCGA 
AACTATGAAC ATCGTCTGCG ATACGAAGAT CGCGTCCCCA TGTATGAAGA ACAGTGGTCA 
TATTATGATG ACAAACAGCC CTACCCGTCT CGGCCACCTT TTGATAATCA GCACTCTCGA 
GACCTTGACT CCAGACAGCA TCCCGAAGAG TCCTCAGAAC GTGGGTACTT TCCACGTTTT 
GAAGAGCCAG CCCCTCTGTC CTATGACAGC AGACCACGTT ATGAACAGGC ACCTAGAGCG 
TCTGCCCTGC GGCACGAAGA GCAGCCAGCT CCTGGGTATG ACACGCATGG TAGACACAGG 
CCGGAAACCC AGCCCTACCC TTCAGCAGGG CCCAAGCCCG CGGAGCCCAA GCAGTATTTT 
GAGCAATATT CACGCAGTTA CGAGCAAGTC CCACCCCAAG GATTTACCTC TAGAGCAGGT 
CATTTTGAGC CTCTCCATGG TGCTGCAGCT GTCCCTCCGC TGATACCTTC ATCTCAGCAT 
AAGCCAGAAG CTCTGCCTTC AAATACCAAA CCGCTGCCTC CACCCCCAAC TCAAACCGAA 
GAAGAGGAAG ATCCAGCAAT GAAGCCACAG TCTGTACTCA CCAGAGTTAA GATGTTTGAA 
AACAAAAGAT CTGCATCCTT AGAGACCAAG AAGGATGTAA ATGACACTGC CACTTTTAAG 
CCTCCAGAAG TAGCATCTAA ACCTTCAGGT GCTCCCATCA TTGGTCCCAA ACCCACTTCT 
CAGAATCAAT TCAGTGAACA TGACAAAACT CTATACAGGA TCCCAGAACC TCAAAAGCCT 
CAACTGAAGC CACCTGAAGA TATCGTTCGG TCAAATCATT ATGACCCTGA AGAAGATGAA 
GAATATTATC GAAAACAGCT CTCATACTTT GACCGAAGAA GTTTTGAGAA TAAGCCTCCT 
GCACACATTG CTGCCAGCCA TCTCTCCGAG CCTGCAAAGC CAGCTCATTC TCAGAATCAA 
TCAAATTTTT CTAGTTATTC TTCAAAGGGA AAGCCTCCTG AAGCTGATGG TGTAGATAGA 
TCGTTTGGTG AGAAACGCTA TGAACCCCTC CAGGTCACTC CCCCTCCTCC TCCATTGCCC 
TCGCAATACG CCCAGCCATC TCAACCTGTC ACCAGCGCGT CTCTCCACAT ACATTCTAAG 
GGAGCACATG GTGAAGGTAA TTCAGTGTCA TTGGATTTTC AGAATTCCTT AGTATCCAAA 
CCAGACCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCAAA CCGAGAAGAT 
ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA AGTTTTCCAG ATAAAGCCCC AGTTAATGGA 
ACTGAACAGA CTCAGAAAAC GGTCACTCCA GCATACAATC GATTCACACC AAAACCATAT 
ACAAGTTCTG CCCGACCGTT TGAACGCAAG TTTGAAAGTC CTAAATTCAA TCACAATCTT 
CTGCCAAGTG AAACTGCGCA TAAACCTGAC TTGTCAAAAA CTCCCACTTC TCCAAAAACT 
CTTGTGAAAT CGCACAGTTT GGCACAGCCT TCTGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTATCCATG CAGAGAAGCC TAAATATCAA ATAAATAATA TCAGCACAGT GCCTAAAACT 
ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG GATGAAGATG AAGATGGTCA TACTGTGGTG 
GCCACAGCGC GAGGCATATT TAACAGCAAT GGTGGCGTGC TGAGTTCCAT AGAAACTGGC 
GTCAGTATAA TTATCCCTCA AGGAGCCATT CCTGAAGGAG TTGAGCAGGA AATCTATTTC 
AAGGTCTGCC GAGACAACAG CATCCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACACTG 
CTGAGTCCCT TGGTGATGTG TGGTCCCCAT GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG 
CGCTTACCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008015414.1
CDS	53..5320
Translation	MEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDKPPRRSRSRSPDQQSEPSDHSRHSPQQPSNGSLRSRDEERVSKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQSQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTTYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVGHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSRDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRHRPETQPYPSAGPKPAEPKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTATFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPLQVTPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSKTPTSPKTLVKSHSLAQPSEFDSGVETFSIHAEKPKYQINNISTVPKTIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_008017223.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008017223.1

ORF Insert Sequence:

ATGGAGGAAA CAGCTATATG GGAACAACAT ACAGTGACGC TTCACAGGGC TCCTGGATTT 
GGATTTGGCA TTGCAATATC TGGTGGACGA GATAATCCTC ATTTTCAGAG TGGGGAAACG 
TCAATCGTGA TTTCAGATGT GCTGAAAGGA GGACCAGCTG AAGGACAGCT ACAGGAAAAT 
GACCGAGTGG CAATGGTTAA CGGAGTTTCA ATGGATAATG TTGAACATGC TTTTGCTGTT 
CAGCAACTAA GGAAAAGTGG GAAAAATGCA AAAATTACAA TTAGAAGGAA GAAGAAAGTT 
CAAATACCAG TAAGTCGTCC TGATCCTGAA CCAGTATCTG ATAATGAAGA AGATAGTTAC 
GATGAGGAAA TACATGATCC AAGAAGTGGC CGGAGTGGTG TGGTTAACAG AAGGAGTGAG 
AAGATTTGGC CGAGGGATAG AAGTGCAAGT AGAGAGAGGA GCTTGTCTCC GCGGTCAGAC 
AGGCGGTCAG TGGCTTCCAG CCAGCCCGCT AAACCCACCA AAGTCACACT GGTTAAATCC 
CGGAAGAATG AAGAATATGG TCTTCGATTG GCAAGCCATA TATTTGTTAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGAAAT ATTCAAGAAG GTGATGTTGT ATTGAAGATA 
AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AGACATTGAT AGAAAGGTCT 
AAAGGCAAAT TAAAAATGGT AGTTCAAAGA GATGAACGGG CTACGCTGTT GAACGTCCCT 
GATCTGTCTG ACAGCATCCA CTCTGCTAAT GCCTCTGAGA GAGACGACAT TTCAGAAATT 
CAGTCACTGG CCTCAGATCA TTCTGGTCGA TCACACGATA AGCCTCCCCG CCGCAGCCGG 
TCACGATCTC CTGACCAGCA GTCAGAGCCT TCTGATCATT CCAGACACTC TCCACAGCAG 
CCAAGCAATG GCAGTCTCCG GAGTAGAGAT GAAGAGAGAG TTTCTAAGCC TGGGGCTGTC 
TCAACTCCTG TAAAGCATGC TGATGATCAC ACACCTAAAA CAGTGGAAGA AGTTACAGTT 
GAAAGAAATG AGAAACAAAC ACCTTCTCTT CCAGAACCAA AGCCTGTGTA TGCCCAGGTT 
GGGCAACCAG ATGTGGATTT ACCTGTCAGT CCATCAGATG GTGTCCTACC TAATTCAACT 
CATGAAGATG GGATTCTTCG ACCCAGCATG AAATTGGTAA AATTCAGAAA AGGAGATAGT 
GTGGGCTTGC GACTGGCTGG TGGAAATGAT GTTGGAATAT TTGTAGCTGG CGTTCTGGAA 
GATAGCCCTG CAGCCAAGGA AGGCTTAGAG GAAGGCGATC AAATTCTCAG GGTAAACAAC 
GTAGATTTTA CAAATATCAT AAGAGAAGAA GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA 
GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTATCGTCG CATTGTAGAA 
TCAGATGTAG GAGATTCTTT CTATATTAGA ACCCATTTTG AATATGAAAA GGAATCTCCC 
TATGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGTGTTG TGGATACCTT GTACAATGGA 
AAACTGGGCT CTTGGCTTGC TATTCGAATT GGCAAAAATC ATAAGGAGGT AGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAGCAG CTAGCCAGTG TACAGTATAC ACTTCCAAAA 
ACAGCAGGGG GAGACCGTGC TGACTTCTGG AGATTCCGAG GTCTTCGCAG CTCCAAGAGA 
AATCTTCGAA AAAGCAGAGA GGATTTGTCT GCTCAGCCTG TTCAAACAAA GTTTCCGGCT 
TATGAAAGAG TGGTTCTTCG AGAAGCTGGA TTTTTGAGGC CTGTAACCAT TTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGCTGGCT AGAGAAGAAC CAGATATTTA TCAAATTGCA 
AAGAGTGAAC CACGAGACGC TGGAACTGAC CAACGTAGCT CTGGCATTAT TCGCCTGCAT 
ACAATAAAGC AAATCATAGA TCAAGACAAA CATGCTTTAT TAGATGTAAC ACCAAATGCA 
GTTGATCGTC TTAACTATGC CCAGTGGTAT CCAATTGTTG TATTTCTTAA TCCTGATTCT 
AAGCAAGGAG TAAAAACAAT GAGAATGAGA TTATGTCCAG AATCTCGGAA AAGTGCCAGG 
AAGTTATATG AGCGATCTCA TAAACTTCGT AAAAATAATC ACCATCTTTT TACAACTACA 
ATTAACTTAA ATTCAATGAA TGATGGTTGG TATGGTGCAC TGAAAGAAGC AATTCAACAA 
CAGCAAAGCC AGCTGGTATG GGTTTCTGAG GGAAAGGCGG ATGGTGCTAC AAGTGATGAC 
CTTGATTTGC ATGATGATCG TCTATCCTAC CTGTCAGCTC CAGGTAGTGA ATACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGACACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC ACCGGAGTCT 
GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGACTCCT CTGGAATGCA TCATGAAAAC 
CAAACATATC CTCCTTACTC ACCACAAGCG CAGCCACAAC CAATTCATAG AATAGACTCC 
CCTGGATTTA AGCCAGCCTC TCAACAGAAA GCAGAAGCTT CATCTCCAGT CCCTTACCTT 
TCGCCTGAAA CAAACCCAGC ATCATCAACC TCTGCTGTTA ATCATAATGT AAATTTAACT 
AATGTCAGAC TGGAGGAGCC CACCCCAGCT CCTTCCACCA CTTACTCACC ACAAGCTGAT 
TCTTTAAGAA CACCAAGTAC TGAGGCAGCT CACATAATGC TAAGAGATCA AGAACCATCA 
TTGTCGTCGC ATGTAGATCC AACAAAGGTG TACAGAAAGG ATCCATATCC CGAGGAAATG 
ATGAGACAAA ACCATGTTTT GAAACAGCCA GCCGTTGGTC ACCCAGGGCA CAGGCCAGAC 
AAAGAGCCTA ATCTGACCTA TGAACCCCAA CTCCCATACG TGGAGAAACA AGCCAGCAGA 
GACCTTGAGC AGCCCACGTA CAGATATGAG TCCTCAAGCT ATACGGACCA GTTTTCTCGA 
AACTATGAAC ATCGTCTGCG ATACGAAGAT CGCGTCCCCA TGTATGAAGA ACAGTGGTCA 
TATTATGATG ACAAACAGCC CTACCCGTCT CGGCCACCTT TTGATAATCA GCACTCTCGA 
GACCTTGACT CCAGACAGCA TCCCGAAGAG TCCTCAGAAC GTGGGTACTT TCCACGTTTT 
GAAGAGCCAG CCCCTCTGTC CTATGACAGC AGACCACGTT ATGAACAGGC ACCTAGAGCG 
TCTGCCCTGC GGCACGAAGA GCAGCCAGCT CCTGGGTATG ACACGCATGG TAGACACAGG 
CCGGAAACCC AGCCCTACCC TTCAGCAGGG CCCAAGCCCG CGGAGCCCAA GCAGTATTTT 
GAGCAATATT CACGCAGTTA CGAGCAAGTC CCACCCCAAG GATTTACCTC TAGAGCAGGT 
CATTTTGAGC CTCTCCATGG TGCTGCAGCT GTCCCTCCGC TGATACCTTC ATCTCAGCAT 
AAGCCAGAAG CTCTGCCTTC AAATACCAAA CCGCTGCCTC CACCCCCAAC TCAAACCGAA 
GAAGAGGAAG ATCCAGCAAT GAAGCCACAG TCTGTACTCA CCAGAGTTAA GATGTTTGAA 
AACAAAAGAT CTGCATCCTT AGAGACCAAG AAGGATGTAA ATGACACTGC CACTTTTAAG 
CCTCCAGAAG TAGCATCTAA ACCTTCAGGT GCTCCCATCA TTGGTCCCAA ACCCACTTCT 
CAGAATCAAT TCAGTGAACA TGACAAAACT CTATACAGGA TCCCAGAACC TCAAAAGCCT 
CAACTGAAGC CACCTGAAGA TATCGTTCGG TCAAATCATT ATGACCCTGA AGAAGATGAA 
GAATATTATC GAAAACAGCT CTCATACTTT GACCGAAGAA GTTTTGAGAA TAAGCCTCCT 
GCACACATTG CTGCCAGCCA TCTCTCCGAG CCTGCAAAGC CAGCTCATTC TCAGAATCAA 
TCAAATTTTT CTAGTTATTC TTCAAAGGGA AAGCCTCCTG AAGCTGATGG TGTAGATAGA 
TCGTTTGGTG AGAAACGCTA TGAACCCCTC CAGGTCACTC CCCCTCCTCC TCCATTGCCC 
TCGCAATACG CCCAGCCATC TCAACCTGTC ACCAGCGCGT CTCTCCACAT ACATTCTAAG 
GGAGCACATG GTGAAGGTAA TTCAGTGTCA TTGGATTTTC AGAATTCCTT AGTATCCAAA 
CCAGACCCAC CTCCGTCTCA GAATAAGCCA GCAACTTTCA GACCACCAAA CCGAGAAGAT 
ACTGCTCAGC CAGCTTTCTA TCCCCAGAAA AGTTTTCCAG ATAAAGCCCC AGTTAATGGA 
ACTGAACAGA CTCAGAAAAC GGTCACTCCA GCATACAATC GATTCACACC AAAACCATAT 
ACAAGTTCTG CCCGACCGTT TGAACGCAAG TTTGAAAGTC CTAAATTCAA TCACAATCTT 
CTGCCAAGTG AAACTGCGCA TAAACCTGAC TTGTCAAAAA CTCCCACTTC TCCAAAAACT 
CTTGTGAAAT CGCACAGTTT GGCACAGCCT TCTGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTATCCATG CAGAGAAGCC TAAATATCAA ATAAATAATA TCAGCACAGT GCCTAAAACT 
ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG GATGAAGATG AAGATGGTCA TACTGTGGTG 
GCCACAGCGC GAGGCATATT TAACAGCAAT GGTGGCGTGC TGAGTTCCAT AGAAACTGGC 
GTCAGTATAA TTATCCCTCA AGGAGCCATT CCTGAAGGAG TTGAGCAGGA AATCTATTTC 
AAGGTCTGCC GAGACAACAG CATCCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACACTG 
CTGAGTCCCT TGGTGATGTG TGGTCCCCAT GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG 
CGCTTACCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_008017224.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008015415.1 Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

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CloneID	OCh123106
Clone ID Related Accession (Same CDS sequence)	XM_008017224.1
Accession Version	XM_008017224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735276.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGCATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TCGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTGGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCTCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CCACCAAAGT CACACTGGTT AAATCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGAAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTGTTGAAC GTCCCTGATC TGTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCCTC AGATCATTCT 
GGTCGATCAC ACGATAAGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCAGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAGTTTC TAAGCCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CAGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGACCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCTTGCGACT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTGGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GCGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGGGGAGA CCGTGCTGAC 
TTCTGGAGAT TCCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCTGCTC AGCCTGTTCA AACAAAGTTT CCGGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTT TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCTAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAATCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGATTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCACTGAA AGAAGCAATT CAACAACAGC AAAGCCAGCT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTA 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCACTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTACA GAAAGGATCC ATATCCCGAG GAAATGATGA GACAAAACCA TGTTTTGAAA 
CAGCCAGCCG TTGGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACGTACAGA 
TATGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCGTCTCGGC CACCTTTTGA TAATCAGCAC TCTCGAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGTGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCCTAT 
GACAGCAGAC CACGTTATGA ACAGGCACCT AGAGCGTCTG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC GCATGGTAGA CACAGGCCGG AAACCCAGCC CTACCCTTCA 
GCAGGGCCCA AGCCCGCGGA GCCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTCCCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAT 
ACCAAACCGC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGCCACT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTAT ACAGGATCCC AGAACCTCAA AAGCCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTCTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCTGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTA GATAGATCGT TTGGTGAGAA ACGCTATGAA 
CCCCTCCAGG TCACTCCCCC TCCTCCTCCA TTGCCCTCGC AATACGCCCA GCCATCTCAA 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTA TCCAAACCAG ACCCACCTCC GTCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACGGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCGTTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCGCATAAA 
CCTGACTTGT CAAAAACTCC CACTTCTCCA AAAACTCTTG TGAAATCGCA CAGTTTGGCA 
CAGCCTTCTG AGTTTGACAG TGGAGTTGAA ACTTTCTCTA TCCATGCAGA GAAGCCTAAA 
TATCAAATAA ATAATATCAG CACAGTGCCT AAAACTATTC CTGTGAGTCC TTCAGCTGTG 
GAAGAGGATG AAGATGAAGA TGGTCATACT GTGGTGGCCA CAGCGCGAGG CATATTTAAC 
AGCAATGGTG GCGTGCTGAG TTCCATAGAA ACTGGCGTCA GTATAATTAT CCCTCAAGGA 
GCCATTCCTG AAGGAGTTGA GCAGGAAATC TATTTCAAGG TCTGCCGAGA CAACAGCATC 
CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACACTGCTGA GTCCCTTGGT GATGTGTGGT 
CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTGCGCT TACCACACTG TGCGTCCATG 
ACTCCTGACG GTGATCCTAA AACCTGGCAA AACAAGTGTC TTCCTGGAGA TCCAAATTAT 
CTCGTTGGAG CAAACTGTGT TTCTGTCCTT ATTGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008015415.1
CDS	418..5682
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDKPPRRSRSRSPDQQSEPSDHSRHSPQQPSNGSLRSRDEERVSKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQSQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTTYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVGHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSRDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRHRPETQPYPSAGPKPAEPKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTATFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPLQVTPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSKTPTSPKTLVKSHSLAQPSEFDSGVETFSIHAEKPKYQINNISTVPKTIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_008017224.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008017224.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGCATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TCGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTGGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCTCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CCACCAAAGT CACACTGGTT AAATCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGAAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTGTTGAAC GTCCCTGATC TGTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCCTC AGATCATTCT 
GGTCGATCAC ACGATAAGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCAGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAGTTTC TAAGCCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CAGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGACCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCTTGCGACT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTGGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GCGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGGGGAGA CCGTGCTGAC 
TTCTGGAGAT TCCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCTGCTC AGCCTGTTCA AACAAAGTTT CCGGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTT TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCTAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAATCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGATTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCACTGAA AGAAGCAATT CAACAACAGC AAAGCCAGCT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTA 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCACTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTACA GAAAGGATCC ATATCCCGAG GAAATGATGA GACAAAACCA TGTTTTGAAA 
CAGCCAGCCG TTGGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACGTACAGA 
TATGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCGTCTCGGC CACCTTTTGA TAATCAGCAC TCTCGAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGTGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCCTAT 
GACAGCAGAC CACGTTATGA ACAGGCACCT AGAGCGTCTG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC GCATGGTAGA CACAGGCCGG AAACCCAGCC CTACCCTTCA 
GCAGGGCCCA AGCCCGCGGA GCCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTCCCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAT 
ACCAAACCGC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGCCACT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTAT ACAGGATCCC AGAACCTCAA AAGCCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTCTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCTGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTA GATAGATCGT TTGGTGAGAA ACGCTATGAA 
CCCCTCCAGG TCACTCCCCC TCCTCCTCCA TTGCCCTCGC AATACGCCCA GCCATCTCAA 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTA TCCAAACCAG ACCCACCTCC GTCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACGGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCGTTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCGCATAAA 
CCTGACTTGT CAAAAACTCC CACTTCTCCA AAAACTCTTG TGAAATCGCA CAGTTTGGCA 
CAGCCTTCTG AGTTTGACAG TGGAGTTGAA ACTTTCTCTA TCCATGCAGA GAAGCCTAAA 
TATCAAATAA ATAATATCAG CACAGTGCCT AAAACTATTC CTGTGAGTCC TTCAGCTGTG 
GAAGAGGATG AAGATGAAGA TGGTCATACT GTGGTGGCCA CAGCGCGAGG CATATTTAAC 
AGCAATGGTG GCGTGCTGAG TTCCATAGAA ACTGGCGTCA GTATAATTAT CCCTCAAGGA 
GCCATTCCTG AAGGAGTTGA GCAGGAAATC TATTTCAAGG TCTGCCGAGA CAACAGCATC 
CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACACTGCTGA GTCCCTTGGT GATGTGTGGT 
CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTGCGCT TACCACACTG TGCGTCCATG 
ACTCCTGACG GTGATCCTAA AACCTGGCAA AACAAGTGTC TTCCTGGAGA TCCAAATTAT 
CTCGTTGGAG CAAACTGTGT TTCTGTCCTT ATTGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_008017225.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008015416.1 Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

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CloneID	OCh123107
Clone ID Related Accession (Same CDS sequence)	XM_008017225.1
Accession Version	XM_008017225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735276.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGCATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TCGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTGGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCTCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CCACCAAAGT CACACTGGTT AAATCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGAAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTGTTGAAC GTCCCTGATC TGTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCCTC AGATCATTCT 
GGTCGATCAC ACGATAAGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCAGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAGTTTC TAAGCCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CAGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGACCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCTTGCGACT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTGGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GCGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGGGGAGA CCGTGCTGAC 
TTCTGGAGAT TCCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCTGCTC AGCCTGTTCA AACAAAGTTT CCGGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTT TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCTAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAATCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGATTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCACTGAA AGAAGCAATT CAACAACAGC AAAGCCAGCT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTA 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCACTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTACA GAAAGGATCC ATATCCCGAG GAAATGATGA GACAAAACCA TGTTTTGAAA 
CAGCCAGCCG TTGGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACGTACAGA 
TATGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCGTCTCGGC CACCTTTTGA TAATCAGCAC TCTCGAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGTGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCCTAT 
GACAGCAGAC CACGTTATGA ACAGGCACCT AGAGCGTCTG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC GCATGGTAGA CACAGGCCGG AAACCCAGCC CTACCCTTCA 
GCAGGGCCCA AGCCCGCGGA GCCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTCCCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAT 
ACCAAACCGC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGCCACT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTAT ACAGGATCCC AGAACCTCAA AAGCCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTCTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCTGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTA GATAGATCGT TTGGTGAGAA ACGCTATGAA 
CCCCTCCAGG TCACTCCCCC TCCTCCTCCA TTGCCCTCGC AATACGCCCA GCCATCTCAA 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTA TCCAAACCAG ACCCACCTCC GTCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACGGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCGTTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCGCATAAA 
CCTGACTTGT CAAAAACTCC CACTTCTCCA AAAACTCTTG TGAAATCGCA CAGTTTGGCA 
CAGCCTTCTG AGTTTGACAG TGGAGTTGAA ACTTTCTCTA TCCATGCAGA GAAGCCTAAA 
TATCAAATAA ATAATATCAG CACAGTGCCT AAAACTATTC CTGTGAGTCC TTCAGCTGTG 
GAAGAGGATG AAGATGAAGA TGGTCATACT GTGGTGGCCA CAGCGCGAGG CATATTTAAC 
AGCAATGGTG GCGTGCTGAG TTCCATAGAA ACTGGCGTCA GTATAATTAT CCCTCAAGGA 
GCCATTCCTG AAGGAGTTGA GCAGGAAATC TATTTCAAGG TCTGCCGAGA CAACAGCATC 
CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACACTGCTGA GTCCCTTGGT GATGTGTGGT 
CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTGCGCT TACCACACTG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTATC TCGTTGGAGC AAACTGTGTT 
TCTGTCCTTA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008015416.1
CDS	470..5713
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDKPPRRSRSRSPDQQSEPSDHSRHSPQQPSNGSLRSRDEERVSKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQSQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTTYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVGHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSRDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRHRPETQPYPSAGPKPAEPKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTATFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPLQVTPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSKTPTSPKTLVKSHSLAQPSEFDSGVETFSIHAEKPKYQINNISTVPKTIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_008017225.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008017225.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGCATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TCGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTGGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCTCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CCACCAAAGT CACACTGGTT AAATCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGAAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTGTTGAAC GTCCCTGATC TGTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCCTC AGATCATTCT 
GGTCGATCAC ACGATAAGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCAGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAGTTTC TAAGCCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CAGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGACCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCTTGCGACT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTGGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GCGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGGGGAGA CCGTGCTGAC 
TTCTGGAGAT TCCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCTGCTC AGCCTGTTCA AACAAAGTTT CCGGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTT TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCTAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAATCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGATTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCACTGAA AGAAGCAATT CAACAACAGC AAAGCCAGCT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTA 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCACTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTACA GAAAGGATCC ATATCCCGAG GAAATGATGA GACAAAACCA TGTTTTGAAA 
CAGCCAGCCG TTGGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACGTACAGA 
TATGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCGTCTCGGC CACCTTTTGA TAATCAGCAC TCTCGAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGTGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCCTAT 
GACAGCAGAC CACGTTATGA ACAGGCACCT AGAGCGTCTG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC GCATGGTAGA CACAGGCCGG AAACCCAGCC CTACCCTTCA 
GCAGGGCCCA AGCCCGCGGA GCCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTCCCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAT 
ACCAAACCGC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGCCACT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTAT ACAGGATCCC AGAACCTCAA AAGCCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTCTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCTGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTA GATAGATCGT TTGGTGAGAA ACGCTATGAA 
CCCCTCCAGG TCACTCCCCC TCCTCCTCCA TTGCCCTCGC AATACGCCCA GCCATCTCAA 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTA TCCAAACCAG ACCCACCTCC GTCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACGGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCGTTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCGCATAAA 
CCTGACTTGT CAAAAACTCC CACTTCTCCA AAAACTCTTG TGAAATCGCA CAGTTTGGCA 
CAGCCTTCTG AGTTTGACAG TGGAGTTGAA ACTTTCTCTA TCCATGCAGA GAAGCCTAAA 
TATCAAATAA ATAATATCAG CACAGTGCCT AAAACTATTC CTGTGAGTCC TTCAGCTGTG 
GAAGAGGATG AAGATGAAGA TGGTCATACT GTGGTGGCCA CAGCGCGAGG CATATTTAAC 
AGCAATGGTG GCGTGCTGAG TTCCATAGAA ACTGGCGTCA GTATAATTAT CCCTCAAGGA 
GCCATTCCTG AAGGAGTTGA GCAGGAAATC TATTTCAAGG TCTGCCGAGA CAACAGCATC 
CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACACTGCTGA GTCCCTTGGT GATGTGTGGT 
CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTGCGCT TACCACACTG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTATC TCGTTGGAGC AAACTGTGTT 
TCTGTCCTTA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_008017226.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008015417.1 Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X6, mRNA.

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CloneID	OCh123108
Clone ID Related Accession (Same CDS sequence)	XM_008017226.1
Accession Version	XM_008017226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735276.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGCATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TCGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTGGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCTCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CCACCAAAGT CACACTGGTT AAATCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGAAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTGTTGAAC GTCCCTGATC TGTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCCTC AGATCATTCT 
GGTCGATCAC ACGATAAGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCAGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAGTTTC TAAGCCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CAGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGACCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCTTGCGACT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTGGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GCGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGGGGAGA CCGTGCTGAC 
TTCTGGAGAT TCCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCTGCTC AGCCTGTTCA AACAAAGTTT CCGGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTT TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCTAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAATCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGATTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCACTGAA AGAAGCAATT CAACAACAGC AAAGCCAGCT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTA 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTACA GAAAGGATCC ATATCCCGAG GAAATGATGA GACAAAACCA TGTTTTGAAA 
CAGCCAGCCG TTGGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACGTACAGA 
TATGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCGTCTCGGC CACCTTTTGA TAATCAGCAC TCTCGAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGTGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCCTAT 
GACAGCAGAC CACGTTATGA ACAGGCACCT AGAGCGTCTG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC GCATGGTAGA CACAGGCCGG AAACCCAGCC CTACCCTTCA 
GCAGGGCCCA AGCCCGCGGA GCCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTCCCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAT 
ACCAAACCGC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGCCACT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTAT ACAGGATCCC AGAACCTCAA AAGCCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTCTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCTGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTA GATAGATCGT TTGGTGAGAA ACGCTATGAA 
CCCCTCCAGG TCACTCCCCC TCCTCCTCCA TTGCCCTCGC AATACGCCCA GCCATCTCAA 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTA TCCAAACCAG ACCCACCTCC GTCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACGGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCGTTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCGCATAAA 
CCTGACTTGT CAAAAACTCC CACTTCTCCA AAAACTCTTG TGAAATCGCA CAGTTTGGCA 
CAGCCTTCTG AGTTTGACAG TGGAGTTGAA ACTTTCTCTA TCCATGCAGA GAAGCCTAAA 
TATCAAATAA ATAATATCAG CACAGTGCCT AAAACTATTC CTGTGAGTCC TTCAGCTGTG 
GAAGAGGATG AAGATGAAGA TGGTCATACT GTGGTGGCCA CAGCGCGAGG CATATTTAAC 
AGCAATGGTG GCGTGCTGAG TTCCATAGAA ACTGGCGTCA GTATAATTAT CCCTCAAGGA 
GCCATTCCTG AAGGAGTTGA GCAGGAAATC TATTTCAAGG TCTGCCGAGA CAACAGCATC 
CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACACTGCTGA GTCCCTTGGT GATGTGTGGT 
CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTGCGCT TACCACACTG TGCGTCCATG 
ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTATC TCGTTGGAGC AAACTGTGTT 
TCTGTCCTTA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008015417.1
CDS	418..5481
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDKPPRRSRSRSPDQQSEPSDHSRHSPQQPSNGSLRSRDEERVSKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQSQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVGHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSRDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRHRPETQPYPSAGPKPAEPKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTATFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPLQVTPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSKTPTSPKTLVKSHSLAQPSEFDSGVETFSIHAEKPKYQINNISTVPKTIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_008017226.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008017226.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGCATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TCGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTGGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCTCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CCACCAAAGT CACACTGGTT AAATCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGAAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTGTTGAAC GTCCCTGATC TGTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCCTC AGATCATTCT 
GGTCGATCAC ACGATAAGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCAGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAGTTTC TAAGCCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CAGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGACCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCTTGCGACT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTGGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GCGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGGGGAGA CCGTGCTGAC 
TTCTGGAGAT TCCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCTGCTC AGCCTGTTCA AACAAAGTTT CCGGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTT TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCTAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAATCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGATTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCACTGAA AGAAGCAATT CAACAACAGC AAAGCCAGCT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTA 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTACA GAAAGGATCC ATATCCCGAG GAAATGATGA GACAAAACCA TGTTTTGAAA 
CAGCCAGCCG TTGGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACGTACAGA 
TATGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCGTCTCGGC CACCTTTTGA TAATCAGCAC TCTCGAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGTGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCCTAT 
GACAGCAGAC CACGTTATGA ACAGGCACCT AGAGCGTCTG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC GCATGGTAGA CACAGGCCGG AAACCCAGCC CTACCCTTCA 
GCAGGGCCCA AGCCCGCGGA GCCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTCCCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAT 
ACCAAACCGC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGCCACT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTAT ACAGGATCCC AGAACCTCAA AAGCCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTCTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCTGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTA GATAGATCGT TTGGTGAGAA ACGCTATGAA 
CCCCTCCAGG TCACTCCCCC TCCTCCTCCA TTGCCCTCGC AATACGCCCA GCCATCTCAA 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTA TCCAAACCAG ACCCACCTCC GTCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACGGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCGTTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCGCATAAA 
CCTGACTTGT CAAAAACTCC CACTTCTCCA AAAACTCTTG TGAAATCGCA CAGTTTGGCA 
CAGCCTTCTG AGTTTGACAG TGGAGTTGAA ACTTTCTCTA TCCATGCAGA GAAGCCTAAA 
TATCAAATAA ATAATATCAG CACAGTGCCT AAAACTATTC CTGTGAGTCC TTCAGCTGTG 
GAAGAGGATG AAGATGAAGA TGGTCATACT GTGGTGGCCA CAGCGCGAGG CATATTTAAC 
AGCAATGGTG GCGTGCTGAG TTCCATAGAA ACTGGCGTCA GTATAATTAT CCCTCAAGGA 
GCCATTCCTG AAGGAGTTGA GCAGGAAATC TATTTCAAGG TCTGCCGAGA CAACAGCATC 
CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACACTGCTGA GTCCCTTGGT GATGTGTGGT 
CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTGCGCT TACCACACTG TGCGTCCATG 
ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTATC TCGTTGGAGC AAACTGTGTT 
TCTGTCCTTA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_008017228.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008015419.1 Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X7, mRNA.

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CloneID	OCh123109
Clone ID Related Accession (Same CDS sequence)	XM_008017228.1
Accession Version	XM_008017228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735276.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGCATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TCGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTGGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCTCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CCACCAAAGT CACACTGGTT AAATCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGAAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTGTTGAAC GTCCCTGATC TGTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCCTC AGATCATTCT 
GGTCGATCAC ACGATAAGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCAGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAGTTTC TAAGCCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CAGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGACCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCTTGCGACT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTGGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GCGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGGGGAGA CCGTGCTGAC 
TTCTGGAGAT TCCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCTGCTC AGCCTGTTCA AACAAAGTTT CCGGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTT TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCTAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAATCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGATTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCACTGAA AGAAGCAATT CAACAACAGC AAAGCCAGCT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTA 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTACA GAAAGGATCC ATATCCCGAG GAAATGATGA GACAAAACCA TGTTTTGAAA 
CAGCCAGCCG TTGGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACGTACAGA 
TATGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCGTCTCGGC CACCTTTTGA TAATCAGCAC TCTCGAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGTGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCCTAT 
GACAGCAGAC CACGTTATGA ACAGGCACCT AGAGCGTCTG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC GCATGGTAGA CACAGGCCGG AAACCCAGCC CTACCCTTCA 
GCAGGGCCCA AGCCCGCGGA GCCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTCCCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAT 
ACCAAACCGC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGCCACT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTAT ACAGGATCCC AGAACCTCAA AAGCCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTCTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCTGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTA GATAGATCGT TTGGTGAGAA ACGCTATGAA 
CCCCTCCAGG TCACTCCCCC TCCTCCTCCA TTGCCCTCGC AATACGCCCA GCCATCTCAA 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTA TCCAAACCAG ACCCACCTCC GTCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACGGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCGTTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCGCATAAA 
CCTGACTTGT CAAAAACTCC CACTTCTCCA AAAACTCTTG TGAAATCGCA CAGTTTGGCA 
CAGCCTTCTG AGTTTGACAG TGGAGTTGAA ACTTTCTCTA TCCATGCAGA GAAGCCTAAA 
TATCAAATAA ATAATATCAG CACAGTGCCT AAAACTATTC CTGTGAGTCC TTCAGCTGTG 
GAAGAGGATG AAGATGAAGA TGGTCATACT GTGGTGGCCA CAGCGCGAGG CATATTTAAC 
AGCAATGGTG GCGTGCTGAG TTCCATAGAA ACTGGCGTCA GTATAATTAT CCCTCAAGGA 
GCCATTCCTG AAGGAGTTGA GCAGGAAATC TATTTCAAGG TCTGCCGAGA CAACAGCATC 
CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACACTGCTGA GTCCCTTGGT GATGTGTGGT 
CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTGCGCT TACCACACTG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTATC TCGTTGGAGC AAACTGTGTT 
TCTGTCCTTA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008015419.1
CDS	470..5473
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDKPPRRSRSRSPDQQSEPSDHSRHSPQQPSNGSLRSRDEERVSKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQSQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVGHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSRDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRHRPETQPYPSAGPKPAEPKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTATFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPLQVTPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQPAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSKTPTSPKTLVKSHSLAQPSEFDSGVETFSIHAEKPKYQINNISTVPKTIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_008017228.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus tight junction protein 1 (TJP1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008017228.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGCATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TCGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTGGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCTCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCCGCTAAAC CCACCAAAGT CACACTGGTT AAATCCCGGA AGAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGAAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTGTTGAAC GTCCCTGATC TGTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCCTC AGATCATTCT 
GGTCGATCAC ACGATAAGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCAGTCA 
GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAGTTTC TAAGCCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAGGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CAGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGACCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GCTTGCGACT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTGGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GCGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGGGGAGA CCGTGCTGAC 
TTCTGGAGAT TCCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCTGCTC AGCCTGTTCA AACAAAGTTT CCGGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTT TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCTAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAATCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGATTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATATGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCACTGAA AGAAGCAATT CAACAACAGC AAAGCCAGCT GGTATGGGTT 
TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTA 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTACA GAAAGGATCC ATATCCCGAG GAAATGATGA GACAAAACCA TGTTTTGAAA 
CAGCCAGCCG TTGGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACGTACAGA 
TATGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCGTCTCGGC CACCTTTTGA TAATCAGCAC TCTCGAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGTGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCCTAT 
GACAGCAGAC CACGTTATGA ACAGGCACCT AGAGCGTCTG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC GCATGGTAGA CACAGGCCGG AAACCCAGCC CTACCCTTCA 
GCAGGGCCCA AGCCCGCGGA GCCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTCCCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAT 
ACCAAACCGC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGCCACT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTAT ACAGGATCCC AGAACCTCAA AAGCCTCAAC TGAAGCCACC TGAAGATATC 
GTTCGGTCAA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTCTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCTGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC TCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTA GATAGATCGT TTGGTGAGAA ACGCTATGAA 
CCCCTCCAGG TCACTCCCCC TCCTCCTCCA TTGCCCTCGC AATACGCCCA GCCATCTCAA 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTA TCCAAACCAG ACCCACCTCC GTCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGCCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACGGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCGTTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCGCATAAA 
CCTGACTTGT CAAAAACTCC CACTTCTCCA AAAACTCTTG TGAAATCGCA CAGTTTGGCA 
CAGCCTTCTG AGTTTGACAG TGGAGTTGAA ACTTTCTCTA TCCATGCAGA GAAGCCTAAA 
TATCAAATAA ATAATATCAG CACAGTGCCT AAAACTATTC CTGTGAGTCC TTCAGCTGTG 
GAAGAGGATG AAGATGAAGA TGGTCATACT GTGGTGGCCA CAGCGCGAGG CATATTTAAC 
AGCAATGGTG GCGTGCTGAG TTCCATAGAA ACTGGCGTCA GTATAATTAT CCCTCAAGGA 
GCCATTCCTG AAGGAGTTGA GCAGGAAATC TATTTCAAGG TCTGCCGAGA CAACAGCATC 
CTCCCACCTT TAGATAAAGA GAAAGGTGAA ACACTGCTGA GTCCCTTGGT GATGTGTGGT 
CCCCATGGCC TCAAGTTCCT GAAGCCTGTG GAGCTGCGCT TACCACACTG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTATC TCGTTGGAGC AAACTGTGTT 
TCTGTCCTTA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TJP1 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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