RAPGEF1 gene cDNA ORF clone, Chlorocebus sabaeus(green monkey)

mRNA	Protein	Name
XM_008005882.1	XP_008004073.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_008005885.1	XP_008004076.1	rap guanine nucleotide exchange factor 1 isoform X6
XM_008005887.1	XP_008004078.1	rap guanine nucleotide exchange factor 1 isoform X8
XM_008005888.1	XP_008004079.1	rap guanine nucleotide exchange factor 1 isoform X9
XM_008005890.1	XP_008004081.1	rap guanine nucleotide exchange factor 1 isoform X11
XM_008005881.1	XP_008004072.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_008005883.1	XP_008004074.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_008005886.1	XP_008004077.1	rap guanine nucleotide exchange factor 1 isoform X7
XM_008005889.1	XP_008004080.1	rap guanine nucleotide exchange factor 1 isoform X10
XM_008005891.1	XP_008004082.1	rap guanine nucleotide exchange factor 1 isoform X12
XM_008005880.1	XP_008004071.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_008005884.1	XP_008004075.1	rap guanine nucleotide exchange factor 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCh98991	XM_008005880.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCh98992	XM_008005881.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCh98993	XM_008005882.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCh98994	XM_008005883.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCh98995	XM_008005884.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCh98996	XM_008005885.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCh98997	XM_008005886.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCh98998	XM_008005887.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCh98999	XM_008005888.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCh99000	XM_008005889.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCh99001	XM_008005890.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCh99002	XM_008005891.1 Latest version!	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

RAPGEF1 ( XM_008005880.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004071.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCh98991
Clone ID Related Accession (Same CDS sequence)	XM_008005880.1
Accession Version	XM_008005880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT 
GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC 
CCCGAGGACG GCAGTGCCGC TCAGGGCCTC AGTGTGTCCG TCTCTAACTC CTTTCTCAGC 
CGGCATGGCA GCTTGCCTGT GCCCTCGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AGCCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAACGCCAGT 
GAGGAAGCTG GTGAGGGTGA ATATGTCAAT CTGTATTCCT CTGGCCAGAG CAGCGAGGAG 
CTGGCTCCTT CTCGAGGAGA ACCACCGGCT GGGAAAGACG GACATTCCAG AGATCCCTCA 
GCAGTCAGCA GCATCCCTGG GAAGGACAGC AGAGACGGCA GTGAGAGGGC CCCAAAGTCA 
CCAGATGCTC TGGAGTCGGC TCAGTCGGAG GAGGAAGTGG ACGAGCTGTC CCTCATCGAC 
CACAACGAAA TTATGTCCAG GCTGACGCTC AAGCAGGAGG GTGATGACGG GCCGGATGTC 
CGCGGAGGAT CTGGTGACAT CTTATTGGTC CATGCTACTG AGACTGACAG GAAAGATTTG 
GTGTTGTACT GCGAGGCCTT CCTGACCACC TACAGGACCT TCATCTCCCC AGAGGAGCTC 
ATCAAGAAGC TGCAGTACAG ATACGAGAAA TTCTCTCCCT TCGCCGATAC GTTCAAGAAG 
CGCGTCAGCA AGAACACATT CTTCGTACTG GTGCGGGTGG TGGATGAGCT TTGCCTGGTG 
GAATTGACAG AAGAGATCCT GAAGCTGCTG ATGGAACTGG TCTTCCGCCT GGTGTGCAAC 
GGGGAGCTGA GCCTGGCCCG CGTGCTCCGG AAGAACATCC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCAC CTCTGGCCAG CCCCTGGCGG CCCGGGGGGT AGCAGCCAGG 
CCGGGGACCT TGCACGACTT TCACAGCCAT GAGATCGCGG AGCAGCTAAC TCTGCTGGAT 
GCTGAGCTCT TCTATAAAAT AGAGATTCCT GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT 
GAGGAAAAGA GCCCCAACTT GACCCAGTTC ACGGAGCACT TCAACAACAT GTCCTACTGG 
GTCCGGTCCA TAATCATGTT ACAGGAAAAG GCCCAGGACA GGGAACGGCT GCTCTTAAAG 
TTCATCAAGA TCATGAAGCA CTTGCGGAAG CTGAATAACT TCAACTCCTA CTTGGCCATC 
CTCTCTGCCC TGGACTCAGC GCCCATCCGC AGGCTGGAGT GGCAGAAGCA GACCTCAGAG 
GGCCTGGCCG AGTACTGCAC GCTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCC 
GCCCTCTCGG AGGTGGAACC GCCGTGCATC CCGTACCTGG GGCTGATCCT GCAGGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGACTAC ATCGATGGGA AAGTGAACTT CTCCAAGCGG 
TGGCAGCAGT TCAACATCCT CGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGACATC 
CGGAGGAACG ACGACATTAT AAACTTCTTC AATGACTTCA GTGACCACCT GGCTGAGGAG 
GCCCTATGGG AACTCTCTCT GAAAATTAAA CCCAGGAACA TAACAAGGAG AAAAACAGAC 
CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004071.1
CDS	970..4767
Translation	MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPAMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005880.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005880.1

ORF Insert Sequence:

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT 
GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC 
CCCGAGGACG GCAGTGCCGC TCAGGGCCTC AGTGTGTCCG TCTCTAACTC CTTTCTCAGC 
CGGCATGGCA GCTTGCCTGT GCCCTCGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AGCCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGAGTGA AAACGCCAGT 
GAGGAAGCTG GTGAGGGTGA ATATGTCAAT CTGTATTCCT CTGGCCAGAG CAGCGAGGAG 
CTGGCTCCTT CTCGAGGAGA ACCACCGGCT GGGAAAGACG GACATTCCAG AGATCCCTCA 
GCAGTCAGCA GCATCCCTGG GAAGGACAGC AGAGACGGCA GTGAGAGGGC CCCAAAGTCA 
CCAGATGCTC TGGAGTCGGC TCAGTCGGAG GAGGAAGTGG ACGAGCTGTC CCTCATCGAC 
CACAACGAAA TTATGTCCAG GCTGACGCTC AAGCAGGAGG GTGATGACGG GCCGGATGTC 
CGCGGAGGAT CTGGTGACAT CTTATTGGTC CATGCTACTG AGACTGACAG GAAAGATTTG 
GTGTTGTACT GCGAGGCCTT CCTGACCACC TACAGGACCT TCATCTCCCC AGAGGAGCTC 
ATCAAGAAGC TGCAGTACAG ATACGAGAAA TTCTCTCCCT TCGCCGATAC GTTCAAGAAG 
CGCGTCAGCA AGAACACATT CTTCGTACTG GTGCGGGTGG TGGATGAGCT TTGCCTGGTG 
GAATTGACAG AAGAGATCCT GAAGCTGCTG ATGGAACTGG TCTTCCGCCT GGTGTGCAAC 
GGGGAGCTGA GCCTGGCCCG CGTGCTCCGG AAGAACATCC TGGACAAGGT GGACCAGAAG 
AAGCTGCTCA GGTGTGCCAC CTCTGGCCAG CCCCTGGCGG CCCGGGGGGT AGCAGCCAGG 
CCGGGGACCT TGCACGACTT TCACAGCCAT GAGATCGCGG AGCAGCTAAC TCTGCTGGAT 
GCTGAGCTCT TCTATAAAAT AGAGATTCCT GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT 
GAGGAAAAGA GCCCCAACTT GACCCAGTTC ACGGAGCACT TCAACAACAT GTCCTACTGG 
GTCCGGTCCA TAATCATGTT ACAGGAAAAG GCCCAGGACA GGGAACGGCT GCTCTTAAAG 
TTCATCAAGA TCATGAAGCA CTTGCGGAAG CTGAATAACT TCAACTCCTA CTTGGCCATC 
CTCTCTGCCC TGGACTCAGC GCCCATCCGC AGGCTGGAGT GGCAGAAGCA GACCTCAGAG 
GGCCTGGCCG AGTACTGCAC GCTGATTGAC AGCTCATCCT CCTTCCGAGC CTACCGGGCC 
GCCCTCTCGG AGGTGGAACC GCCGTGCATC CCGTACCTGG GGCTGATCCT GCAGGACCTG 
ACCTTCGTTC ACCTGGGAAA CCCAGACTAC ATCGATGGGA AAGTGAACTT CTCCAAGCGG 
TGGCAGCAGT TCAACATCCT CGACAGCATG CGGTGCTTCC AGCAGGCGCA CTATGACATC 
CGGAGGAACG ACGACATTAT AAACTTCTTC AATGACTTCA GTGACCACCT GGCTGAGGAG 
GCCCTATGGG AACTCTCTCT GAAAATTAAA CCCAGGAACA TAACAAGGAG AAAAACAGAC 
CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_008005881.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004072.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCh98992
Clone ID Related Accession (Same CDS sequence)	XM_008005881.1
Accession Version	XM_008005881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTCTC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
CTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCAATTG ATAAGAAGGT ACTGGAGATG 
CTTCCAGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAACGATCCT 
CGAATTCAGC ACAGCTCAGC CCTGTCTTCC TGCTATAGCC GAGTGTACCA AAGTCTCGCC 
AACCTCATTC GCTGGTCTGA CCAAGTGATG CTGGAAGGTG TGAACTCAGA AGACAAGGAG 
ATGGTGACGA CGGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGGCTCACCG TCGAGAAGCA GGGACGTCCG TCTCCAACAA GCCCCGTGAA GCCCAGTTCC 
CCTGCTGGCA AGCCTGATGG CCCAGCAGAG CTCCCCCTGA CAGACCGCGA GGTAGAAATC 
CTAAACAAGA CGACCGGGAT GTCACAGTCA ACTGAGCTCC TCCCAGACGC CACAGATGAA 
GAGGTCGCGC CCCCCAAGCC CCCTCTGCCT GGCATTCGGG TGGTTGATAA TAGTCCTCCA 
CCAGCATTGC CACCCAAGAA AAGACAGTCG GCACCGTCCC CTACCCGAGT GGCTGTGGTG 
GCCCCCATGA GCCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAT 
TTTGACGTTG ACTGTTATGC ACAGAGGCGA CTGTCAGGAG GCAGCCACTC ATATGGTGGA 
GAGTCGCCCC GCCTCTCCCC CTGCAGCAGC ATAGGCAAGC TCAGCAAGTC AGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGTGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GACCACTATG ATCCCGACTA TGAATTCCTC CAGCAAGACC TCTCTAACGC AGACCAGATA 
CCTCAGCAGA CGGCCTGGAA CCTTAGCCCG TTGCCAGAGT CTTTGGGGGA GTCTGGGTCT 
CCATTTGTTG GCCATCCTTT CCAGCTGCCT CTTGGCAGCC ATCCCCAGCC AGACGGACCT 
CTGGCCCCAG GGCAGCAGAC AGTTACGCCG CCTGCTCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACGGCGGA CAGCTCTGGC TGCAGGGTGT CCTACGAGCG GCATCCCTCG 
CAGTACGACA ACATCTCTGG GGAGGACCTG CAGAGCACGG CCCCAATCCA GTCCGTCCCC 
TACGCGCCCT TTGCTGCTAT TCTCCCCTTT CAGCATGGAG GTTCCTCAGC CCCTGTCGAA 
TTTGTGGGTG ATTTTACTGC TCCTGAATCA ACGGGTGACC CAGAAAAACC ACCTCCTCTA 
CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTGGAGGA CTACTCGGAG 
CCACAGCCCT CCATGTTCTA CCAGACGCCG CAGAACGAGC ACATCTACCA GCAGAAGAAC 
AAGCTCCTCA TGGAGGTATA CGGCTTCAGC GACTCCTTCA GCGGGGTGGA CTCTGTGCAG 
GAGCTGGCCC CGCCGCCCGC CCTACCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CATGGCAGCT TGCCTGTGCC CTCGTATAAG TCTGTGTTTA GGTCCTACTC CCAGGATTTC 
GTGCCTCACC ACCAAGCTTC CGTTCCGCCT TTCCTTCCGC CTACCTCCTC TTCCTCTCCA 
CATTTCCCAC CTGCCCACCA GTCTCAGAGC TCTGACTTAG CCGTGCCAGC CATGGCCGGT 
CCACCTCCCA GCACCGTGGA CGGGCCTCTC TCGGCTTCTC AGGAGAGCAG CTTTCATGGG 
AATACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGAGTGAAAA CGCCAGTGAG 
GAAGCTGGTG AGGGTGAATA TGTCAATCTG TATTCCTCTG GCCAGAGCAG CGAGGAGCTG 
GCTCCTTCTC GAGGAGAACC ACCGGCTGGG AAAGACGGAC ATTCCAGAGA TCCCTCAGCA 
GTCAGCAGCA TCCCTGGGAA GGACAGCAGA GACGGCAGTG AGAGGGCCCC AAAGTCACCA 
GATGCTCTGG AGTCGGCTCA GTCGGAGGAG GAAGTGGACG AGCTGTCCCT CATCGACCAC 
AACGAAATTA TGTCCAGGCT GACGCTCAAG CAGGAGGGTG ATGACGGGCC GGATGTCCGC 
GGAGGATCTG GTGACATCTT ATTGGTCCAT GCTACTGAGA CTGACAGGAA AGATTTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCTCCCCAGA GGAGCTCATC 
AAGAAGCTGC AGTACAGATA CGAGAAATTC TCTCCCTTCG CCGATACGTT CAAGAAGCGC 
GTCAGCAAGA ACACATTCTT CGTACTGGTG CGGGTGGTGG ATGAGCTTTG CCTGGTGGAA 
TTGACAGAAG AGATCCTGAA GCTGCTGATG GAACTGGTCT TCCGCCTGGT GTGCAACGGG 
GAGCTGAGCC TGGCCCGCGT GCTCCGGAAG AACATCCTGG ACAAGGTGGA CCAGAAGAAG 
CTGCTCAGGT GTGCCACCTC TGGCCAGCCC CTGGCGGCCC GGGGGGTAGC AGCCAGGCCG 
GGGACCTTGC ACGACTTTCA CAGCCATGAG ATCGCGGAGC AGCTAACTCT GCTGGATGCT 
GAGCTCTTCT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCAAAAGA GCAGAATGAG 
GAAAAGAGCC CCAACTTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCCATAA TCATGTTACA GGAAAAGGCC CAGGACAGGG AACGGCTGCT CTTAAAGTTC 
ATCAAGATCA TGAAGCACTT GCGGAAGCTG AATAACTTCA ACTCCTACTT GGCCATCCTC 
TCTGCCCTGG ACTCAGCGCC CATCCGCAGG CTGGAGTGGC AGAAGCAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCCGCC 
CTCTCGGAGG TGGAACCGCC GTGCATCCCG TACCTGGGGC TGATCCTGCA GGACCTGACC 
TTCGTTCACC TGGGAAACCC AGACTACATC GATGGGAAAG TGAACTTCTC CAAGCGGTGG 
CAGCAGTTCA ACATCCTCGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA TGACATCCGG 
AGGAACGACG ACATTATAAA CTTCTTCAAT GACTTCAGTG ACCACCTGGC TGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATTAAACCC AGGAACATAA CAAGGAGAAA AACAGACCGG 
GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004072.1
CDS	90..3884
Translation	MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPAMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005881.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005881.1

ORF Insert Sequence:

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTCTC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
CTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCAATTG ATAAGAAGGT ACTGGAGATG 
CTTCCAGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAACGATCCT 
CGAATTCAGC ACAGCTCAGC CCTGTCTTCC TGCTATAGCC GAGTGTACCA AAGTCTCGCC 
AACCTCATTC GCTGGTCTGA CCAAGTGATG CTGGAAGGTG TGAACTCAGA AGACAAGGAG 
ATGGTGACGA CGGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGGCTCACCG TCGAGAAGCA GGGACGTCCG TCTCCAACAA GCCCCGTGAA GCCCAGTTCC 
CCTGCTGGCA AGCCTGATGG CCCAGCAGAG CTCCCCCTGA CAGACCGCGA GGTAGAAATC 
CTAAACAAGA CGACCGGGAT GTCACAGTCA ACTGAGCTCC TCCCAGACGC CACAGATGAA 
GAGGTCGCGC CCCCCAAGCC CCCTCTGCCT GGCATTCGGG TGGTTGATAA TAGTCCTCCA 
CCAGCATTGC CACCCAAGAA AAGACAGTCG GCACCGTCCC CTACCCGAGT GGCTGTGGTG 
GCCCCCATGA GCCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAT 
TTTGACGTTG ACTGTTATGC ACAGAGGCGA CTGTCAGGAG GCAGCCACTC ATATGGTGGA 
GAGTCGCCCC GCCTCTCCCC CTGCAGCAGC ATAGGCAAGC TCAGCAAGTC AGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGTGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GACCACTATG ATCCCGACTA TGAATTCCTC CAGCAAGACC TCTCTAACGC AGACCAGATA 
CCTCAGCAGA CGGCCTGGAA CCTTAGCCCG TTGCCAGAGT CTTTGGGGGA GTCTGGGTCT 
CCATTTGTTG GCCATCCTTT CCAGCTGCCT CTTGGCAGCC ATCCCCAGCC AGACGGACCT 
CTGGCCCCAG GGCAGCAGAC AGTTACGCCG CCTGCTCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACGGCGGA CAGCTCTGGC TGCAGGGTGT CCTACGAGCG GCATCCCTCG 
CAGTACGACA ACATCTCTGG GGAGGACCTG CAGAGCACGG CCCCAATCCA GTCCGTCCCC 
TACGCGCCCT TTGCTGCTAT TCTCCCCTTT CAGCATGGAG GTTCCTCAGC CCCTGTCGAA 
TTTGTGGGTG ATTTTACTGC TCCTGAATCA ACGGGTGACC CAGAAAAACC ACCTCCTCTA 
CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTGGAGGA CTACTCGGAG 
CCACAGCCCT CCATGTTCTA CCAGACGCCG CAGAACGAGC ACATCTACCA GCAGAAGAAC 
AAGCTCCTCA TGGAGGTATA CGGCTTCAGC GACTCCTTCA GCGGGGTGGA CTCTGTGCAG 
GAGCTGGCCC CGCCGCCCGC CCTACCCCCC AAGCAGCGGC AGCTGCAGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CATGGCAGCT TGCCTGTGCC CTCGTATAAG TCTGTGTTTA GGTCCTACTC CCAGGATTTC 
GTGCCTCACC ACCAAGCTTC CGTTCCGCCT TTCCTTCCGC CTACCTCCTC TTCCTCTCCA 
CATTTCCCAC CTGCCCACCA GTCTCAGAGC TCTGACTTAG CCGTGCCAGC CATGGCCGGT 
CCACCTCCCA GCACCGTGGA CGGGCCTCTC TCGGCTTCTC AGGAGAGCAG CTTTCATGGG 
AATACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGAGTGAAAA CGCCAGTGAG 
GAAGCTGGTG AGGGTGAATA TGTCAATCTG TATTCCTCTG GCCAGAGCAG CGAGGAGCTG 
GCTCCTTCTC GAGGAGAACC ACCGGCTGGG AAAGACGGAC ATTCCAGAGA TCCCTCAGCA 
GTCAGCAGCA TCCCTGGGAA GGACAGCAGA GACGGCAGTG AGAGGGCCCC AAAGTCACCA 
GATGCTCTGG AGTCGGCTCA GTCGGAGGAG GAAGTGGACG AGCTGTCCCT CATCGACCAC 
AACGAAATTA TGTCCAGGCT GACGCTCAAG CAGGAGGGTG ATGACGGGCC GGATGTCCGC 
GGAGGATCTG GTGACATCTT ATTGGTCCAT GCTACTGAGA CTGACAGGAA AGATTTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCTCCCCAGA GGAGCTCATC 
AAGAAGCTGC AGTACAGATA CGAGAAATTC TCTCCCTTCG CCGATACGTT CAAGAAGCGC 
GTCAGCAAGA ACACATTCTT CGTACTGGTG CGGGTGGTGG ATGAGCTTTG CCTGGTGGAA 
TTGACAGAAG AGATCCTGAA GCTGCTGATG GAACTGGTCT TCCGCCTGGT GTGCAACGGG 
GAGCTGAGCC TGGCCCGCGT GCTCCGGAAG AACATCCTGG ACAAGGTGGA CCAGAAGAAG 
CTGCTCAGGT GTGCCACCTC TGGCCAGCCC CTGGCGGCCC GGGGGGTAGC AGCCAGGCCG 
GGGACCTTGC ACGACTTTCA CAGCCATGAG ATCGCGGAGC AGCTAACTCT GCTGGATGCT 
GAGCTCTTCT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCAAAAGA GCAGAATGAG 
GAAAAGAGCC CCAACTTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCCATAA TCATGTTACA GGAAAAGGCC CAGGACAGGG AACGGCTGCT CTTAAAGTTC 
ATCAAGATCA TGAAGCACTT GCGGAAGCTG AATAACTTCA ACTCCTACTT GGCCATCCTC 
TCTGCCCTGG ACTCAGCGCC CATCCGCAGG CTGGAGTGGC AGAAGCAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCCGCC 
CTCTCGGAGG TGGAACCGCC GTGCATCCCG TACCTGGGGC TGATCCTGCA GGACCTGACC 
TTCGTTCACC TGGGAAACCC AGACTACATC GATGGGAAAG TGAACTTCTC CAAGCGGTGG 
CAGCAGTTCA ACATCCTCGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA TGACATCCGG 
AGGAACGACG ACATTATAAA CTTCTTCAAT GACTTCAGTG ACCACCTGGC TGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATTAAACCC AGGAACATAA CAAGGAGAAA AACAGACCGG 
GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_008005882.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004073.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X3, mRNA.

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CloneID	OCh98993
Clone ID Related Accession (Same CDS sequence)	XM_008005882.1
Accession Version	XM_008005882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CATGGCAGCT TGCCTGTGCC CTCGTATAAG TCTGTGTTTA GGTCCTACTC CCAGGATTTC 
GTGCCTCACC ACCAAGCTTC CGTTCCGCCT TTCCTTCCGC CTACCTCCTC TTCCTCTCCA 
CATTTCCCAC CTGCCCACCA GTCTCAGAGC TCTGACTTAG CCGTGCCAGC CATGGCCGGT 
CCACCTCCCA GCACCGTGGA CGGGCCTCTC TCGGCTTCTC AGGAGAGCAG CTTTCATGGG 
AATACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGAGTGAAAA CGCCAGTGAG 
GAAGCTGGTG AGGGTGAATA TGTCAATCTG TATTCCTCTG GCCAGAGCAG CGAGGAGCTG 
GCTCCTTCTC GAGGAGAACC ACCGGCTGGG AAAGACGGAC ATTCCAGAGA TCCCTCAGCA 
GTCAGCAGCA TCCCTGGGAA GGACAGCAGA GACGGCAGTG AGAGGGCCCC AAAGTCACCA 
GATGCTCTGG AGTCGGCTCA GTCGGAGGAG GAAGTGGACG AGCTGTCCCT CATCGACCAC 
AACGAAATTA TGTCCAGGCT GACGCTCAAG CAGGAGGGTG ATGACGGGCC GGATGTCCGC 
GGAGGATCTG GTGACATCTT ATTGGTCCAT GCTACTGAGA CTGACAGGAA AGATTTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCTCCCCAGA GGAGCTCATC 
AAGAAGCTGC AGTACAGATA CGAGAAATTC TCTCCCTTCG CCGATACGTT CAAGAAGCGC 
GTCAGCAAGA ACACATTCTT CGTACTGGTG CGGGTGGTGG ATGAGCTTTG CCTGGTGGAA 
TTGACAGAAG AGATCCTGAA GCTGCTGATG GAACTGGTCT TCCGCCTGGT GTGCAACGGG 
GAGCTGAGCC TGGCCCGCGT GCTCCGGAAG AACATCCTGG ACAAGGTGGA CCAGAAGAAG 
CTGCTCAGGT GTGCCACCTC TGGCCAGCCC CTGGCGGCCC GGGGGGTAGC AGCCAGGCCG 
GGGACCTTGC ACGACTTTCA CAGCCATGAG ATCGCGGAGC AGCTAACTCT GCTGGATGCT 
GAGCTCTTCT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCAAAAGA GCAGAATGAG 
GAAAAGAGCC CCAACTTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCCATAA TCATGTTACA GGAAAAGGCC CAGGACAGGG AACGGCTGCT CTTAAAGTTC 
ATCAAGATCA TGAAGCACTT GCGGAAGCTG AATAACTTCA ACTCCTACTT GGCCATCCTC 
TCTGCCCTGG ACTCAGCGCC CATCCGCAGG CTGGAGTGGC AGAAGCAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCCGCC 
CTCTCGGAGG TGGAACCGCC GTGCATCCCG TACCTGGGGC TGATCCTGCA GGACCTGACC 
TTCGTTCACC TGGGAAACCC AGACTACATC GATGGGAAAG TGAACTTCTC CAAGCGGTGG 
CAGCAGTTCA ACATCCTCGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA TGACATCCGG 
AGGAACGACG ACATTATAAA CTTCTTCAAT GACTTCAGTG ACCACCTGGC TGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATTAAACCC AGGAACATAA CAAGGAGAAA AACAGACCGG 
GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004073.1
CDS	970..4764
Translation	MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPAMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005882.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005882.1

ORF Insert Sequence:

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CATGGCAGCT TGCCTGTGCC CTCGTATAAG TCTGTGTTTA GGTCCTACTC CCAGGATTTC 
GTGCCTCACC ACCAAGCTTC CGTTCCGCCT TTCCTTCCGC CTACCTCCTC TTCCTCTCCA 
CATTTCCCAC CTGCCCACCA GTCTCAGAGC TCTGACTTAG CCGTGCCAGC CATGGCCGGT 
CCACCTCCCA GCACCGTGGA CGGGCCTCTC TCGGCTTCTC AGGAGAGCAG CTTTCATGGG 
AATACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGAGTGAAAA CGCCAGTGAG 
GAAGCTGGTG AGGGTGAATA TGTCAATCTG TATTCCTCTG GCCAGAGCAG CGAGGAGCTG 
GCTCCTTCTC GAGGAGAACC ACCGGCTGGG AAAGACGGAC ATTCCAGAGA TCCCTCAGCA 
GTCAGCAGCA TCCCTGGGAA GGACAGCAGA GACGGCAGTG AGAGGGCCCC AAAGTCACCA 
GATGCTCTGG AGTCGGCTCA GTCGGAGGAG GAAGTGGACG AGCTGTCCCT CATCGACCAC 
AACGAAATTA TGTCCAGGCT GACGCTCAAG CAGGAGGGTG ATGACGGGCC GGATGTCCGC 
GGAGGATCTG GTGACATCTT ATTGGTCCAT GCTACTGAGA CTGACAGGAA AGATTTGGTG 
TTGTACTGCG AGGCCTTCCT GACCACCTAC AGGACCTTCA TCTCCCCAGA GGAGCTCATC 
AAGAAGCTGC AGTACAGATA CGAGAAATTC TCTCCCTTCG CCGATACGTT CAAGAAGCGC 
GTCAGCAAGA ACACATTCTT CGTACTGGTG CGGGTGGTGG ATGAGCTTTG CCTGGTGGAA 
TTGACAGAAG AGATCCTGAA GCTGCTGATG GAACTGGTCT TCCGCCTGGT GTGCAACGGG 
GAGCTGAGCC TGGCCCGCGT GCTCCGGAAG AACATCCTGG ACAAGGTGGA CCAGAAGAAG 
CTGCTCAGGT GTGCCACCTC TGGCCAGCCC CTGGCGGCCC GGGGGGTAGC AGCCAGGCCG 
GGGACCTTGC ACGACTTTCA CAGCCATGAG ATCGCGGAGC AGCTAACTCT GCTGGATGCT 
GAGCTCTTCT ATAAAATAGA GATTCCTGAG GTTTTGCTTT GGGCAAAAGA GCAGAATGAG 
GAAAAGAGCC CCAACTTGAC CCAGTTCACG GAGCACTTCA ACAACATGTC CTACTGGGTC 
CGGTCCATAA TCATGTTACA GGAAAAGGCC CAGGACAGGG AACGGCTGCT CTTAAAGTTC 
ATCAAGATCA TGAAGCACTT GCGGAAGCTG AATAACTTCA ACTCCTACTT GGCCATCCTC 
TCTGCCCTGG ACTCAGCGCC CATCCGCAGG CTGGAGTGGC AGAAGCAGAC CTCAGAGGGC 
CTGGCCGAGT ACTGCACGCT GATTGACAGC TCATCCTCCT TCCGAGCCTA CCGGGCCGCC 
CTCTCGGAGG TGGAACCGCC GTGCATCCCG TACCTGGGGC TGATCCTGCA GGACCTGACC 
TTCGTTCACC TGGGAAACCC AGACTACATC GATGGGAAAG TGAACTTCTC CAAGCGGTGG 
CAGCAGTTCA ACATCCTCGA CAGCATGCGG TGCTTCCAGC AGGCGCACTA TGACATCCGG 
AGGAACGACG ACATTATAAA CTTCTTCAAT GACTTCAGTG ACCACCTGGC TGAGGAGGCC 
CTATGGGAAC TCTCTCTGAA AATTAAACCC AGGAACATAA CAAGGAGAAA AACAGACCGG 
GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_008005883.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004074.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCh98994
Clone ID Related Accession (Same CDS sequence)	XM_008005883.1
Accession Version	XM_008005883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT 
GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC 
CCCGAGGACG GCAGTGCCGC TCAGGGCCTC AGTGTGTCCG TCTCTAACTC CTTTCTCAGC 
CGGCATGGCA GCTTGCCTGT GCCCTCGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AGCCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGGAACC ACCGGCTGGG 
AAAGACGGAC ATTCCAGAGA TCCCTCAGCA GTCAGCAGCA TCCCTGGGAA GGACAGCAGA 
GACGGCAGTG AGAGGGCCCC AAAGTCACCA GATGCTCTGG AGTCGGCTCA GTCGGAGGAG 
GAAGTGGACG AGCTGTCCCT CATCGACCAC AACGAAATTA TGTCCAGGCT GACGCTCAAG 
CAGGAGGGTG ATGACGGGCC GGATGTCCGC GGAGGATCTG GTGACATCTT ATTGGTCCAT 
GCTACTGAGA CTGACAGGAA AGATTTGGTG TTGTACTGCG AGGCCTTCCT GACCACCTAC 
AGGACCTTCA TCTCCCCAGA GGAGCTCATC AAGAAGCTGC AGTACAGATA CGAGAAATTC 
TCTCCCTTCG CCGATACGTT CAAGAAGCGC GTCAGCAAGA ACACATTCTT CGTACTGGTG 
CGGGTGGTGG ATGAGCTTTG CCTGGTGGAA TTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAACTGGTCT TCCGCCTGGT GTGCAACGGG GAGCTGAGCC TGGCCCGCGT GCTCCGGAAG 
AACATCCTGG ACAAGGTGGA CCAGAAGAAG CTGCTCAGGT GTGCCACCTC TGGCCAGCCC 
CTGGCGGCCC GGGGGGTAGC AGCCAGGCCG GGGACCTTGC ACGACTTTCA CAGCCATGAG 
ATCGCGGAGC AGCTAACTCT GCTGGATGCT GAGCTCTTCT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAG GAAAAGAGCC CCAACTTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCCATAA TCATGTTACA GGAAAAGGCC 
CAGGACAGGG AACGGCTGCT CTTAAAGTTC ATCAAGATCA TGAAGCACTT GCGGAAGCTG 
AATAACTTCA ACTCCTACTT GGCCATCCTC TCTGCCCTGG ACTCAGCGCC CATCCGCAGG 
CTGGAGTGGC AGAAGCAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATTGACAGC 
TCATCCTCCT TCCGAGCCTA CCGGGCCGCC CTCTCGGAGG TGGAACCGCC GTGCATCCCG 
TACCTGGGGC TGATCCTGCA GGACCTGACC TTCGTTCACC TGGGAAACCC AGACTACATC 
GATGGGAAAG TGAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTCGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA TGACATCCGG AGGAACGACG ACATTATAAA CTTCTTCAAT 
GACTTCAGTG ACCACCTGGC TGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATTAAACCC 
AGGAACATAA CAAGGAGAAA AACAGACCGG GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004074.1
CDS	970..4674
Translation	MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPAMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005883.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005883.1

ORF Insert Sequence:

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT 
GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC 
CCCGAGGACG GCAGTGCCGC TCAGGGCCTC AGTGTGTCCG TCTCTAACTC CTTTCTCAGC 
CGGCATGGCA GCTTGCCTGT GCCCTCGTAT AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT 
TTCGTGCCTC ACCACCAAGC TTCCGTTCCG CCTTTCCTTC CGCCTACCTC CTCTTCCTCT 
CCACATTTCC CACCTGCCCA CCAGTCTCAG AGCTCTGACT TAGCCGTGCC AGCCATGGCC 
GGTCCACCTC CCAGCACCGT GGACGGGCCT CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT 
GGGAATACTG TCTGCCTTCC TTCCGAAACC TCTTTCACTG ACTCGGAACC ACCGGCTGGG 
AAAGACGGAC ATTCCAGAGA TCCCTCAGCA GTCAGCAGCA TCCCTGGGAA GGACAGCAGA 
GACGGCAGTG AGAGGGCCCC AAAGTCACCA GATGCTCTGG AGTCGGCTCA GTCGGAGGAG 
GAAGTGGACG AGCTGTCCCT CATCGACCAC AACGAAATTA TGTCCAGGCT GACGCTCAAG 
CAGGAGGGTG ATGACGGGCC GGATGTCCGC GGAGGATCTG GTGACATCTT ATTGGTCCAT 
GCTACTGAGA CTGACAGGAA AGATTTGGTG TTGTACTGCG AGGCCTTCCT GACCACCTAC 
AGGACCTTCA TCTCCCCAGA GGAGCTCATC AAGAAGCTGC AGTACAGATA CGAGAAATTC 
TCTCCCTTCG CCGATACGTT CAAGAAGCGC GTCAGCAAGA ACACATTCTT CGTACTGGTG 
CGGGTGGTGG ATGAGCTTTG CCTGGTGGAA TTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAACTGGTCT TCCGCCTGGT GTGCAACGGG GAGCTGAGCC TGGCCCGCGT GCTCCGGAAG 
AACATCCTGG ACAAGGTGGA CCAGAAGAAG CTGCTCAGGT GTGCCACCTC TGGCCAGCCC 
CTGGCGGCCC GGGGGGTAGC AGCCAGGCCG GGGACCTTGC ACGACTTTCA CAGCCATGAG 
ATCGCGGAGC AGCTAACTCT GCTGGATGCT GAGCTCTTCT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAG GAAAAGAGCC CCAACTTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCCATAA TCATGTTACA GGAAAAGGCC 
CAGGACAGGG AACGGCTGCT CTTAAAGTTC ATCAAGATCA TGAAGCACTT GCGGAAGCTG 
AATAACTTCA ACTCCTACTT GGCCATCCTC TCTGCCCTGG ACTCAGCGCC CATCCGCAGG 
CTGGAGTGGC AGAAGCAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATTGACAGC 
TCATCCTCCT TCCGAGCCTA CCGGGCCGCC CTCTCGGAGG TGGAACCGCC GTGCATCCCG 
TACCTGGGGC TGATCCTGCA GGACCTGACC TTCGTTCACC TGGGAAACCC AGACTACATC 
GATGGGAAAG TGAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTCGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA TGACATCCGG AGGAACGACG ACATTATAAA CTTCTTCAAT 
GACTTCAGTG ACCACCTGGC TGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATTAAACCC 
AGGAACATAA CAAGGAGAAA AACAGACCGG GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_008005884.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004075.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCh98995
Clone ID Related Accession (Same CDS sequence)	XM_008005884.1
Accession Version	XM_008005884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CATGGCAGCT TGCCTGTGCC CTCGTATAAG TCTGTGTTTA GGTCCTACTC CCAGGATTTC 
GTGCCTCACC ACCAAGCTTC CGTTCCGCCT TTCCTTCCGC CTACCTCCTC TTCCTCTCCA 
CATTTCCCAC CTGCCCACCA GTCTCAGAGC TCTGACTTAG CCGTGCCAGC CATGGCCGGT 
CCACCTCCCA GCACCGTGGA CGGGCCTCTC TCGGCTTCTC AGGAGAGCAG CTTTCATGGG 
AATACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGGAACCACC GGCTGGGAAA 
GACGGACATT CCAGAGATCC CTCAGCAGTC AGCAGCATCC CTGGGAAGGA CAGCAGAGAC 
GGCAGTGAGA GGGCCCCAAA GTCACCAGAT GCTCTGGAGT CGGCTCAGTC GGAGGAGGAA 
GTGGACGAGC TGTCCCTCAT CGACCACAAC GAAATTATGT CCAGGCTGAC GCTCAAGCAG 
GAGGGTGATG ACGGGCCGGA TGTCCGCGGA GGATCTGGTG ACATCTTATT GGTCCATGCT 
ACTGAGACTG ACAGGAAAGA TTTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCT CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGATACGA GAAATTCTCT 
CCCTTCGCCG ATACGTTCAA GAAGCGCGTC AGCAAGAACA CATTCTTCGT ACTGGTGCGG 
GTGGTGGATG AGCTTTGCCT GGTGGAATTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAA 
CTGGTCTTCC GCCTGGTGTG CAACGGGGAG CTGAGCCTGG CCCGCGTGCT CCGGAAGAAC 
ATCCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCACCTCTGG CCAGCCCCTG 
GCGGCCCGGG GGGTAGCAGC CAGGCCGGGG ACCTTGCACG ACTTTCACAG CCATGAGATC 
GCGGAGCAGC TAACTCTGCT GGATGCTGAG CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CAAAAGAGCA GAATGAGGAA AAGAGCCCCA ACTTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCCATAATCA TGTTACAGGA AAAGGCCCAG 
GACAGGGAAC GGCTGCTCTT AAAGTTCATC AAGATCATGA AGCACTTGCG GAAGCTGAAT 
AACTTCAACT CCTACTTGGC CATCCTCTCT GCCCTGGACT CAGCGCCCAT CCGCAGGCTG 
GAGTGGCAGA AGCAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT TGACAGCTCA 
TCCTCCTTCC GAGCCTACCG GGCCGCCCTC TCGGAGGTGG AACCGCCGTG CATCCCGTAC 
CTGGGGCTGA TCCTGCAGGA CCTGACCTTC GTTCACCTGG GAAACCCAGA CTACATCGAT 
GGGAAAGTGA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTCGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTATGA CATCCGGAGG AACGACGACA TTATAAACTT CTTCAATGAC 
TTCAGTGACC ACCTGGCTGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT TAAACCCAGG 
AACATAACAA GGAGAAAAAC AGACCGGGAA GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004075.1
CDS	970..4671
Translation	MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPAMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005884.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005884.1

ORF Insert Sequence:

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CATGGCAGCT TGCCTGTGCC CTCGTATAAG TCTGTGTTTA GGTCCTACTC CCAGGATTTC 
GTGCCTCACC ACCAAGCTTC CGTTCCGCCT TTCCTTCCGC CTACCTCCTC TTCCTCTCCA 
CATTTCCCAC CTGCCCACCA GTCTCAGAGC TCTGACTTAG CCGTGCCAGC CATGGCCGGT 
CCACCTCCCA GCACCGTGGA CGGGCCTCTC TCGGCTTCTC AGGAGAGCAG CTTTCATGGG 
AATACTGTCT GCCTTCCTTC CGAAACCTCT TTCACTGACT CGGAACCACC GGCTGGGAAA 
GACGGACATT CCAGAGATCC CTCAGCAGTC AGCAGCATCC CTGGGAAGGA CAGCAGAGAC 
GGCAGTGAGA GGGCCCCAAA GTCACCAGAT GCTCTGGAGT CGGCTCAGTC GGAGGAGGAA 
GTGGACGAGC TGTCCCTCAT CGACCACAAC GAAATTATGT CCAGGCTGAC GCTCAAGCAG 
GAGGGTGATG ACGGGCCGGA TGTCCGCGGA GGATCTGGTG ACATCTTATT GGTCCATGCT 
ACTGAGACTG ACAGGAAAGA TTTGGTGTTG TACTGCGAGG CCTTCCTGAC CACCTACAGG 
ACCTTCATCT CCCCAGAGGA GCTCATCAAG AAGCTGCAGT ACAGATACGA GAAATTCTCT 
CCCTTCGCCG ATACGTTCAA GAAGCGCGTC AGCAAGAACA CATTCTTCGT ACTGGTGCGG 
GTGGTGGATG AGCTTTGCCT GGTGGAATTG ACAGAAGAGA TCCTGAAGCT GCTGATGGAA 
CTGGTCTTCC GCCTGGTGTG CAACGGGGAG CTGAGCCTGG CCCGCGTGCT CCGGAAGAAC 
ATCCTGGACA AGGTGGACCA GAAGAAGCTG CTCAGGTGTG CCACCTCTGG CCAGCCCCTG 
GCGGCCCGGG GGGTAGCAGC CAGGCCGGGG ACCTTGCACG ACTTTCACAG CCATGAGATC 
GCGGAGCAGC TAACTCTGCT GGATGCTGAG CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
TTGCTTTGGG CAAAAGAGCA GAATGAGGAA AAGAGCCCCA ACTTGACCCA GTTCACGGAG 
CACTTCAACA ACATGTCCTA CTGGGTCCGG TCCATAATCA TGTTACAGGA AAAGGCCCAG 
GACAGGGAAC GGCTGCTCTT AAAGTTCATC AAGATCATGA AGCACTTGCG GAAGCTGAAT 
AACTTCAACT CCTACTTGGC CATCCTCTCT GCCCTGGACT CAGCGCCCAT CCGCAGGCTG 
GAGTGGCAGA AGCAGACCTC AGAGGGCCTG GCCGAGTACT GCACGCTGAT TGACAGCTCA 
TCCTCCTTCC GAGCCTACCG GGCCGCCCTC TCGGAGGTGG AACCGCCGTG CATCCCGTAC 
CTGGGGCTGA TCCTGCAGGA CCTGACCTTC GTTCACCTGG GAAACCCAGA CTACATCGAT 
GGGAAAGTGA ACTTCTCCAA GCGGTGGCAG CAGTTCAACA TCCTCGACAG CATGCGGTGC 
TTCCAGCAGG CGCACTATGA CATCCGGAGG AACGACGACA TTATAAACTT CTTCAATGAC 
TTCAGTGACC ACCTGGCTGA GGAGGCCCTA TGGGAACTCT CTCTGAAAAT TAAACCCAGG 
AACATAACAA GGAGAAAAAC AGACCGGGAA GAGAAGACCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_008005885.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004076.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X6, mRNA.

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CloneID	OCh98996
Clone ID Related Accession (Same CDS sequence)	XM_008005885.1
Accession Version	XM_008005885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAAACCTG AGCTGGCTGG AGGAGAAAGA GAAGGAAGTT 
GTCAGTGCCC TGCGCTACTT TAAGACCATT GTGGACAAAA TGGCAATTGA TAAGAAGGTA 
CTGGAGATGC TTCCAGGGTC AGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG 
AACGATCCTC GAATTCAGCA CAGCTCAGCC CTGTCTTCCT GCTATAGCCG AGTGTACCAA 
AGTCTCGCCA ACCTCATTCG CTGGTCTGAC CAAGTGATGC TGGAAGGTGT GAACTCAGAA 
GACAAGGAGA TGGTGACGAC GGTGAAGGGG GTCATCAAGG CCGTGCTGGA TGGAGTGAAG 
GAGCTGGTCA GGCTCACCGT CGAGAAGCAG GGACGTCCGT CTCCAACAAG CCCCGTGAAG 
CCCAGTTCCC CTGCTGGCAA GCCTGATGGC CCAGCAGAGC TCCCCCTGAC AGACCGCGAG 
GTAGAAATCC TAAACAAGAC GACCGGGATG TCACAGTCAA CTGAGCTCCT CCCAGACGCC 
ACAGATGAAG AGGTCGCGCC CCCCAAGCCC CCTCTGCCTG GCATTCGGGT GGTTGATAAT 
AGTCCTCCAC CAGCATTGCC ACCCAAGAAA AGACAGTCGG CACCGTCCCC TACCCGAGTG 
GCTGTGGTGG CCCCCATGAG CCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATCAAT 
AGGCAGGATT TTGACGTTGA CTGTTATGCA CAGAGGCGAC TGTCAGGAGG CAGCCACTCA 
TATGGTGGAG AGTCGCCCCG CCTCTCCCCC TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGTGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ACCACTATGA TCCCGACTAT GAATTCCTCC AGCAAGACCT CTCTAACGCA 
GACCAGATAC CTCAGCAGAC GGCCTGGAAC CTTAGCCCGT TGCCAGAGTC TTTGGGGGAG 
TCTGGGTCTC CATTTGTTGG CCATCCTTTC CAGCTGCCTC TTGGCAGCCA TCCCCAGCCA 
GACGGACCTC TGGCCCCAGG GCAGCAGACA GTTACGCCGC CTGCTCTGCC CGAGAAGAAG 
CGCAGGAGTG CAGCCTCCCA GACGGCGGAC AGCTCTGGCT GCAGGGTGTC CTACGAGCGG 
CATCCCTCGC AGTACGACAA CATCTCTGGG GAGGACCTGC AGAGCACGGC CCCAATCCAG 
TCCGTCCCCT ACGCGCCCTT TGCTGCTATT CTCCCCTTTC AGCATGGAGG TTCCTCAGCC 
CCTGTCGAAT TTGTGGGTGA TTTTACTGCT CCTGAATCAA CGGGTGACCC AGAAAAACCA 
CCTCCTCTAC CAGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGCT GCTGGAGGAC 
TACTCGGAGC CACAGCCCTC CATGTTCTAC CAGACGCCGC AGAACGAGCA CATCTACCAG 
CAGAAGAACA AGCTCCTCAT GGAGGTATAC GGCTTCAGCG ACTCCTTCAG CGGGGTGGAC 
TCTGTGCAGG AGCTGGCCCC GCCGCCCGCC CTACCCCCCA AGCAGCGGCA GCTGCAGGCC 
TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCCTCAGTG TGTCCGTCTC TAACTCCTTT 
CTCAGCCGGC ATGGCAGCTT GCCTGTGCCC TCGTATAAGT CTGTGTTTAG GTCCTACTCC 
CAGGATTTCG TGCCTCACCA CCAAGCTTCC GTTCCGCCTT TCCTTCCGCC TACCTCCTCT 
TCCTCTCCAC ATTTCCCACC TGCCCACCAG TCTCAGAGCT CTGACTTAGC CGTGCCAGCC 
ATGGCCGGTC CACCTCCCAG CACCGTGGAC GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC 
TTTCATGGGA ATACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GAGTGAAAAC 
GCCAGTGAGG AAGCTGGTGA GGGTGAATAT GTCAATCTGT ATTCCTCTGG CCAGAGCAGC 
GAGGAGCTGG CTCCTTCTCG AGGAGAACCA CCGGCTGGGA AAGACGGACA TTCCAGAGAT 
CCCTCAGCAG TCAGCAGCAT CCCTGGGAAG GACAGCAGAG ACGGCAGTGA GAGGGCCCCA 
AAGTCACCAG ATGCTCTGGA GTCGGCTCAG TCGGAGGAGG AAGTGGACGA GCTGTCCCTC 
ATCGACCACA ACGAAATTAT GTCCAGGCTG ACGCTCAAGC AGGAGGGTGA TGACGGGCCG 
GATGTCCGCG GAGGATCTGG TGACATCTTA TTGGTCCATG CTACTGAGAC TGACAGGAAA 
GATTTGGTGT TGTACTGCGA GGCCTTCCTG ACCACCTACA GGACCTTCAT CTCCCCAGAG 
GAGCTCATCA AGAAGCTGCA GTACAGATAC GAGAAATTCT CTCCCTTCGC CGATACGTTC 
AAGAAGCGCG TCAGCAAGAA CACATTCTTC GTACTGGTGC GGGTGGTGGA TGAGCTTTGC 
CTGGTGGAAT TGACAGAAGA GATCCTGAAG CTGCTGATGG AACTGGTCTT CCGCCTGGTG 
TGCAACGGGG AGCTGAGCCT GGCCCGCGTG CTCCGGAAGA ACATCCTGGA CAAGGTGGAC 
CAGAAGAAGC TGCTCAGGTG TGCCACCTCT GGCCAGCCCC TGGCGGCCCG GGGGGTAGCA 
GCCAGGCCGG GGACCTTGCA CGACTTTCAC AGCCATGAGA TCGCGGAGCA GCTAACTCTG 
CTGGATGCTG AGCTCTTCTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG 
CAGAATGAGG AAAAGAGCCC CAACTTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCCATAAT CATGTTACAG GAAAAGGCCC AGGACAGGGA ACGGCTGCTC 
TTAAAGTTCA TCAAGATCAT GAAGCACTTG CGGAAGCTGA ATAACTTCAA CTCCTACTTG 
GCCATCCTCT CTGCCCTGGA CTCAGCGCCC ATCCGCAGGC TGGAGTGGCA GAAGCAGACC 
TCAGAGGGCC TGGCCGAGTA CTGCACGCTG ATTGACAGCT CATCCTCCTT CCGAGCCTAC 
CGGGCCGCCC TCTCGGAGGT GGAACCGCCG TGCATCCCGT ACCTGGGGCT GATCCTGCAG 
GACCTGACCT TCGTTCACCT GGGAAACCCA GACTACATCG ATGGGAAAGT GAACTTCTCC 
AAGCGGTGGC AGCAGTTCAA CATCCTCGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAT 
GACATCCGGA GGAACGACGA CATTATAAAC TTCTTCAATG ACTTCAGTGA CCACCTGGCT 
GAGGAGGCCC TATGGGAACT CTCTCTGAAA ATTAAACCCA GGAACATAAC AAGGAGAAAA 
ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004076.1
CDS	970..4653
Translation	MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPAMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005885.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005885.1

ORF Insert Sequence:

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAAACCTG AGCTGGCTGG AGGAGAAAGA GAAGGAAGTT 
GTCAGTGCCC TGCGCTACTT TAAGACCATT GTGGACAAAA TGGCAATTGA TAAGAAGGTA 
CTGGAGATGC TTCCAGGGTC AGCCAGCAAG GTGCTGGAGG CCATCTTACC CCTGGTGCAG 
AACGATCCTC GAATTCAGCA CAGCTCAGCC CTGTCTTCCT GCTATAGCCG AGTGTACCAA 
AGTCTCGCCA ACCTCATTCG CTGGTCTGAC CAAGTGATGC TGGAAGGTGT GAACTCAGAA 
GACAAGGAGA TGGTGACGAC GGTGAAGGGG GTCATCAAGG CCGTGCTGGA TGGAGTGAAG 
GAGCTGGTCA GGCTCACCGT CGAGAAGCAG GGACGTCCGT CTCCAACAAG CCCCGTGAAG 
CCCAGTTCCC CTGCTGGCAA GCCTGATGGC CCAGCAGAGC TCCCCCTGAC AGACCGCGAG 
GTAGAAATCC TAAACAAGAC GACCGGGATG TCACAGTCAA CTGAGCTCCT CCCAGACGCC 
ACAGATGAAG AGGTCGCGCC CCCCAAGCCC CCTCTGCCTG GCATTCGGGT GGTTGATAAT 
AGTCCTCCAC CAGCATTGCC ACCCAAGAAA AGACAGTCGG CACCGTCCCC TACCCGAGTG 
GCTGTGGTGG CCCCCATGAG CCGAGCCACC AGTGGCTCCA GTTTGCCTGT TGGAATCAAT 
AGGCAGGATT TTGACGTTGA CTGTTATGCA CAGAGGCGAC TGTCAGGAGG CAGCCACTCA 
TATGGTGGAG AGTCGCCCCG CCTCTCCCCC TGCAGCAGCA TAGGCAAGCT CAGCAAGTCA 
GACGAGCAGC TGTCCTCTCT GGACAGGGAC AGTGGGCAGT GCTCCCGGAA CACAAGCTGT 
GAAACACTAG ACCACTATGA TCCCGACTAT GAATTCCTCC AGCAAGACCT CTCTAACGCA 
GACCAGATAC CTCAGCAGAC GGCCTGGAAC CTTAGCCCGT TGCCAGAGTC TTTGGGGGAG 
TCTGGGTCTC CATTTGTTGG CCATCCTTTC CAGCTGCCTC TTGGCAGCCA TCCCCAGCCA 
GACGGACCTC TGGCCCCAGG GCAGCAGACA GTTACGCCGC CTGCTCTGCC CGAGAAGAAG 
CGCAGGAGTG CAGCCTCCCA GACGGCGGAC AGCTCTGGCT GCAGGGTGTC CTACGAGCGG 
CATCCCTCGC AGTACGACAA CATCTCTGGG GAGGACCTGC AGAGCACGGC CCCAATCCAG 
TCCGTCCCCT ACGCGCCCTT TGCTGCTATT CTCCCCTTTC AGCATGGAGG TTCCTCAGCC 
CCTGTCGAAT TTGTGGGTGA TTTTACTGCT CCTGAATCAA CGGGTGACCC AGAAAAACCA 
CCTCCTCTAC CAGAGAAGAA AAACAAACAC ATGCTGGCCT ACATGCAGCT GCTGGAGGAC 
TACTCGGAGC CACAGCCCTC CATGTTCTAC CAGACGCCGC AGAACGAGCA CATCTACCAG 
CAGAAGAACA AGCTCCTCAT GGAGGTATAC GGCTTCAGCG ACTCCTTCAG CGGGGTGGAC 
TCTGTGCAGG AGCTGGCCCC GCCGCCCGCC CTACCCCCCA AGCAGCGGCA GCTGCAGGCC 
TCCTGTGCTG CTTCTTCCTT CTCCTCTGTC TCCTACTGTG TCCAGCAAAC TAAAGTGGCC 
TTCACCCCCG AGGACGGCAG TGCCGCTCAG GGCCTCAGTG TGTCCGTCTC TAACTCCTTT 
CTCAGCCGGC ATGGCAGCTT GCCTGTGCCC TCGTATAAGT CTGTGTTTAG GTCCTACTCC 
CAGGATTTCG TGCCTCACCA CCAAGCTTCC GTTCCGCCTT TCCTTCCGCC TACCTCCTCT 
TCCTCTCCAC ATTTCCCACC TGCCCACCAG TCTCAGAGCT CTGACTTAGC CGTGCCAGCC 
ATGGCCGGTC CACCTCCCAG CACCGTGGAC GGGCCTCTCT CGGCTTCTCA GGAGAGCAGC 
TTTCATGGGA ATACTGTCTG CCTTCCTTCC GAAACCTCTT TCACTGACTC GAGTGAAAAC 
GCCAGTGAGG AAGCTGGTGA GGGTGAATAT GTCAATCTGT ATTCCTCTGG CCAGAGCAGC 
GAGGAGCTGG CTCCTTCTCG AGGAGAACCA CCGGCTGGGA AAGACGGACA TTCCAGAGAT 
CCCTCAGCAG TCAGCAGCAT CCCTGGGAAG GACAGCAGAG ACGGCAGTGA GAGGGCCCCA 
AAGTCACCAG ATGCTCTGGA GTCGGCTCAG TCGGAGGAGG AAGTGGACGA GCTGTCCCTC 
ATCGACCACA ACGAAATTAT GTCCAGGCTG ACGCTCAAGC AGGAGGGTGA TGACGGGCCG 
GATGTCCGCG GAGGATCTGG TGACATCTTA TTGGTCCATG CTACTGAGAC TGACAGGAAA 
GATTTGGTGT TGTACTGCGA GGCCTTCCTG ACCACCTACA GGACCTTCAT CTCCCCAGAG 
GAGCTCATCA AGAAGCTGCA GTACAGATAC GAGAAATTCT CTCCCTTCGC CGATACGTTC 
AAGAAGCGCG TCAGCAAGAA CACATTCTTC GTACTGGTGC GGGTGGTGGA TGAGCTTTGC 
CTGGTGGAAT TGACAGAAGA GATCCTGAAG CTGCTGATGG AACTGGTCTT CCGCCTGGTG 
TGCAACGGGG AGCTGAGCCT GGCCCGCGTG CTCCGGAAGA ACATCCTGGA CAAGGTGGAC 
CAGAAGAAGC TGCTCAGGTG TGCCACCTCT GGCCAGCCCC TGGCGGCCCG GGGGGTAGCA 
GCCAGGCCGG GGACCTTGCA CGACTTTCAC AGCCATGAGA TCGCGGAGCA GCTAACTCTG 
CTGGATGCTG AGCTCTTCTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG 
CAGAATGAGG AAAAGAGCCC CAACTTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCCATAAT CATGTTACAG GAAAAGGCCC AGGACAGGGA ACGGCTGCTC 
TTAAAGTTCA TCAAGATCAT GAAGCACTTG CGGAAGCTGA ATAACTTCAA CTCCTACTTG 
GCCATCCTCT CTGCCCTGGA CTCAGCGCCC ATCCGCAGGC TGGAGTGGCA GAAGCAGACC 
TCAGAGGGCC TGGCCGAGTA CTGCACGCTG ATTGACAGCT CATCCTCCTT CCGAGCCTAC 
CGGGCCGCCC TCTCGGAGGT GGAACCGCCG TGCATCCCGT ACCTGGGGCT GATCCTGCAG 
GACCTGACCT TCGTTCACCT GGGAAACCCA GACTACATCG ATGGGAAAGT GAACTTCTCC 
AAGCGGTGGC AGCAGTTCAA CATCCTCGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAT 
GACATCCGGA GGAACGACGA CATTATAAAC TTCTTCAATG ACTTCAGTGA CCACCTGGCT 
GAGGAGGCCC TATGGGAACT CTCTCTGAAA ATTAAACCCA GGAACATAAC AAGGAGAAAA 
ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_008005886.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004077.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X7, mRNA.

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CloneID	OCh98997
Clone ID Related Accession (Same CDS sequence)	XM_008005886.1
Accession Version	XM_008005886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT 
GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC 
CCCGAGGACG GCAGTGCCGC TCAGGGCCTC AGTGTGTCCG TCTCTAACTC CTTTCTCAGC 
CGGCATGGCA GCTTGCCTGT GCCCTCGGAA CCACCGGCTG GGAAAGACGG ACATTCCAGA 
GATCCCTCAG CAGTCAGCAG CATCCCTGGG AAGGACAGCA GAGACGGCAG TGAGAGGGCC 
CCAAAGTCAC CAGATGCTCT GGAGTCGGCT CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC 
CTCATCGACC ACAACGAAAT TATGTCCAGG CTGACGCTCA AGCAGGAGGG TGATGACGGG 
CCGGATGTCC GCGGAGGATC TGGTGACATC TTATTGGTCC ATGCTACTGA GACTGACAGG 
AAAGATTTGG TGTTGTACTG CGAGGCCTTC CTGACCACCT ACAGGACCTT CATCTCCCCA 
GAGGAGCTCA TCAAGAAGCT GCAGTACAGA TACGAGAAAT TCTCTCCCTT CGCCGATACG 
TTCAAGAAGC GCGTCAGCAA GAACACATTC TTCGTACTGG TGCGGGTGGT GGATGAGCTT 
TGCCTGGTGG AATTGACAGA AGAGATCCTG AAGCTGCTGA TGGAACTGGT CTTCCGCCTG 
GTGTGCAACG GGGAGCTGAG CCTGGCCCGC GTGCTCCGGA AGAACATCCT GGACAAGGTG 
GACCAGAAGA AGCTGCTCAG GTGTGCCACC TCTGGCCAGC CCCTGGCGGC CCGGGGGGTA 
GCAGCCAGGC CGGGGACCTT GCACGACTTT CACAGCCATG AGATCGCGGA GCAGCTAACT 
CTGCTGGATG CTGAGCTCTT CTATAAAATA GAGATTCCTG AGGTTTTGCT TTGGGCAAAA 
GAGCAGAATG AGGAAAAGAG CCCCAACTTG ACCCAGTTCA CGGAGCACTT CAACAACATG 
TCCTACTGGG TCCGGTCCAT AATCATGTTA CAGGAAAAGG CCCAGGACAG GGAACGGCTG 
CTCTTAAAGT TCATCAAGAT CATGAAGCAC TTGCGGAAGC TGAATAACTT CAACTCCTAC 
TTGGCCATCC TCTCTGCCCT GGACTCAGCG CCCATCCGCA GGCTGGAGTG GCAGAAGCAG 
ACCTCAGAGG GCCTGGCCGA GTACTGCACG CTGATTGACA GCTCATCCTC CTTCCGAGCC 
TACCGGGCCG CCCTCTCGGA GGTGGAACCG CCGTGCATCC CGTACCTGGG GCTGATCCTG 
CAGGACCTGA CCTTCGTTCA CCTGGGAAAC CCAGACTACA TCGATGGGAA AGTGAACTTC 
TCCAAGCGGT GGCAGCAGTT CAACATCCTC GACAGCATGC GGTGCTTCCA GCAGGCGCAC 
TATGACATCC GGAGGAACGA CGACATTATA AACTTCTTCA ATGACTTCAG TGACCACCTG 
GCTGAGGAGG CCCTATGGGA ACTCTCTCTG AAAATTAAAC CCAGGAACAT AACAAGGAGA 
AAAACAGACC GGGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004077.1
CDS	968..4414
Translation	MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005886.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005886.1

ORF Insert Sequence:

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT 
GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC TGTGTCCAGC AAACTAAAGT GGCCTTCACC 
CCCGAGGACG GCAGTGCCGC TCAGGGCCTC AGTGTGTCCG TCTCTAACTC CTTTCTCAGC 
CGGCATGGCA GCTTGCCTGT GCCCTCGGAA CCACCGGCTG GGAAAGACGG ACATTCCAGA 
GATCCCTCAG CAGTCAGCAG CATCCCTGGG AAGGACAGCA GAGACGGCAG TGAGAGGGCC 
CCAAAGTCAC CAGATGCTCT GGAGTCGGCT CAGTCGGAGG AGGAAGTGGA CGAGCTGTCC 
CTCATCGACC ACAACGAAAT TATGTCCAGG CTGACGCTCA AGCAGGAGGG TGATGACGGG 
CCGGATGTCC GCGGAGGATC TGGTGACATC TTATTGGTCC ATGCTACTGA GACTGACAGG 
AAAGATTTGG TGTTGTACTG CGAGGCCTTC CTGACCACCT ACAGGACCTT CATCTCCCCA 
GAGGAGCTCA TCAAGAAGCT GCAGTACAGA TACGAGAAAT TCTCTCCCTT CGCCGATACG 
TTCAAGAAGC GCGTCAGCAA GAACACATTC TTCGTACTGG TGCGGGTGGT GGATGAGCTT 
TGCCTGGTGG AATTGACAGA AGAGATCCTG AAGCTGCTGA TGGAACTGGT CTTCCGCCTG 
GTGTGCAACG GGGAGCTGAG CCTGGCCCGC GTGCTCCGGA AGAACATCCT GGACAAGGTG 
GACCAGAAGA AGCTGCTCAG GTGTGCCACC TCTGGCCAGC CCCTGGCGGC CCGGGGGGTA 
GCAGCCAGGC CGGGGACCTT GCACGACTTT CACAGCCATG AGATCGCGGA GCAGCTAACT 
CTGCTGGATG CTGAGCTCTT CTATAAAATA GAGATTCCTG AGGTTTTGCT TTGGGCAAAA 
GAGCAGAATG AGGAAAAGAG CCCCAACTTG ACCCAGTTCA CGGAGCACTT CAACAACATG 
TCCTACTGGG TCCGGTCCAT AATCATGTTA CAGGAAAAGG CCCAGGACAG GGAACGGCTG 
CTCTTAAAGT TCATCAAGAT CATGAAGCAC TTGCGGAAGC TGAATAACTT CAACTCCTAC 
TTGGCCATCC TCTCTGCCCT GGACTCAGCG CCCATCCGCA GGCTGGAGTG GCAGAAGCAG 
ACCTCAGAGG GCCTGGCCGA GTACTGCACG CTGATTGACA GCTCATCCTC CTTCCGAGCC 
TACCGGGCCG CCCTCTCGGA GGTGGAACCG CCGTGCATCC CGTACCTGGG GCTGATCCTG 
CAGGACCTGA CCTTCGTTCA CCTGGGAAAC CCAGACTACA TCGATGGGAA AGTGAACTTC 
TCCAAGCGGT GGCAGCAGTT CAACATCCTC GACAGCATGC GGTGCTTCCA GCAGGCGCAC 
TATGACATCC GGAGGAACGA CGACATTATA AACTTCTTCA ATGACTTCAG TGACCACCTG 
GCTGAGGAGG CCCTATGGGA ACTCTCTCTG AAAATTAAAC CCAGGAACAT AACAAGGAGA 
AAAACAGACC GGGAAGAGAA GACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_008005887.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004078.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCh98998
Clone ID Related Accession (Same CDS sequence)	XM_008005887.1
Accession Version	XM_008005887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CATGGCAGCT TGCCTGTGCC CTCGGAACCA CCGGCTGGGA AAGACGGACA TTCCAGAGAT 
CCCTCAGCAG TCAGCAGCAT CCCTGGGAAG GACAGCAGAG ACGGCAGTGA GAGGGCCCCA 
AAGTCACCAG ATGCTCTGGA GTCGGCTCAG TCGGAGGAGG AAGTGGACGA GCTGTCCCTC 
ATCGACCACA ACGAAATTAT GTCCAGGCTG ACGCTCAAGC AGGAGGGTGA TGACGGGCCG 
GATGTCCGCG GAGGATCTGG TGACATCTTA TTGGTCCATG CTACTGAGAC TGACAGGAAA 
GATTTGGTGT TGTACTGCGA GGCCTTCCTG ACCACCTACA GGACCTTCAT CTCCCCAGAG 
GAGCTCATCA AGAAGCTGCA GTACAGATAC GAGAAATTCT CTCCCTTCGC CGATACGTTC 
AAGAAGCGCG TCAGCAAGAA CACATTCTTC GTACTGGTGC GGGTGGTGGA TGAGCTTTGC 
CTGGTGGAAT TGACAGAAGA GATCCTGAAG CTGCTGATGG AACTGGTCTT CCGCCTGGTG 
TGCAACGGGG AGCTGAGCCT GGCCCGCGTG CTCCGGAAGA ACATCCTGGA CAAGGTGGAC 
CAGAAGAAGC TGCTCAGGTG TGCCACCTCT GGCCAGCCCC TGGCGGCCCG GGGGGTAGCA 
GCCAGGCCGG GGACCTTGCA CGACTTTCAC AGCCATGAGA TCGCGGAGCA GCTAACTCTG 
CTGGATGCTG AGCTCTTCTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG 
CAGAATGAGG AAAAGAGCCC CAACTTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCCATAAT CATGTTACAG GAAAAGGCCC AGGACAGGGA ACGGCTGCTC 
TTAAAGTTCA TCAAGATCAT GAAGCACTTG CGGAAGCTGA ATAACTTCAA CTCCTACTTG 
GCCATCCTCT CTGCCCTGGA CTCAGCGCCC ATCCGCAGGC TGGAGTGGCA GAAGCAGACC 
TCAGAGGGCC TGGCCGAGTA CTGCACGCTG ATTGACAGCT CATCCTCCTT CCGAGCCTAC 
CGGGCCGCCC TCTCGGAGGT GGAACCGCCG TGCATCCCGT ACCTGGGGCT GATCCTGCAG 
GACCTGACCT TCGTTCACCT GGGAAACCCA GACTACATCG ATGGGAAAGT GAACTTCTCC 
AAGCGGTGGC AGCAGTTCAA CATCCTCGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAT 
GACATCCGGA GGAACGACGA CATTATAAAC TTCTTCAATG ACTTCAGTGA CCACCTGGCT 
GAGGAGGCCC TATGGGAACT CTCTCTGAAA ATTAAACCCA GGAACATAAC AAGGAGAAAA 
ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004078.1
CDS	968..4411
Translation	MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005887.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005887.1

ORF Insert Sequence:

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGGC CTCCTGTGCT 
GCTTCTTCCT TCTCCTCTGT CTCCTACTGT GTCCAGCAAA CTAAAGTGGC CTTCACCCCC 
GAGGACGGCA GTGCCGCTCA GGGCCTCAGT GTGTCCGTCT CTAACTCCTT TCTCAGCCGG 
CATGGCAGCT TGCCTGTGCC CTCGGAACCA CCGGCTGGGA AAGACGGACA TTCCAGAGAT 
CCCTCAGCAG TCAGCAGCAT CCCTGGGAAG GACAGCAGAG ACGGCAGTGA GAGGGCCCCA 
AAGTCACCAG ATGCTCTGGA GTCGGCTCAG TCGGAGGAGG AAGTGGACGA GCTGTCCCTC 
ATCGACCACA ACGAAATTAT GTCCAGGCTG ACGCTCAAGC AGGAGGGTGA TGACGGGCCG 
GATGTCCGCG GAGGATCTGG TGACATCTTA TTGGTCCATG CTACTGAGAC TGACAGGAAA 
GATTTGGTGT TGTACTGCGA GGCCTTCCTG ACCACCTACA GGACCTTCAT CTCCCCAGAG 
GAGCTCATCA AGAAGCTGCA GTACAGATAC GAGAAATTCT CTCCCTTCGC CGATACGTTC 
AAGAAGCGCG TCAGCAAGAA CACATTCTTC GTACTGGTGC GGGTGGTGGA TGAGCTTTGC 
CTGGTGGAAT TGACAGAAGA GATCCTGAAG CTGCTGATGG AACTGGTCTT CCGCCTGGTG 
TGCAACGGGG AGCTGAGCCT GGCCCGCGTG CTCCGGAAGA ACATCCTGGA CAAGGTGGAC 
CAGAAGAAGC TGCTCAGGTG TGCCACCTCT GGCCAGCCCC TGGCGGCCCG GGGGGTAGCA 
GCCAGGCCGG GGACCTTGCA CGACTTTCAC AGCCATGAGA TCGCGGAGCA GCTAACTCTG 
CTGGATGCTG AGCTCTTCTA TAAAATAGAG ATTCCTGAGG TTTTGCTTTG GGCAAAAGAG 
CAGAATGAGG AAAAGAGCCC CAACTTGACC CAGTTCACGG AGCACTTCAA CAACATGTCC 
TACTGGGTCC GGTCCATAAT CATGTTACAG GAAAAGGCCC AGGACAGGGA ACGGCTGCTC 
TTAAAGTTCA TCAAGATCAT GAAGCACTTG CGGAAGCTGA ATAACTTCAA CTCCTACTTG 
GCCATCCTCT CTGCCCTGGA CTCAGCGCCC ATCCGCAGGC TGGAGTGGCA GAAGCAGACC 
TCAGAGGGCC TGGCCGAGTA CTGCACGCTG ATTGACAGCT CATCCTCCTT CCGAGCCTAC 
CGGGCCGCCC TCTCGGAGGT GGAACCGCCG TGCATCCCGT ACCTGGGGCT GATCCTGCAG 
GACCTGACCT TCGTTCACCT GGGAAACCCA GACTACATCG ATGGGAAAGT GAACTTCTCC 
AAGCGGTGGC AGCAGTTCAA CATCCTCGAC AGCATGCGGT GCTTCCAGCA GGCGCACTAT 
GACATCCGGA GGAACGACGA CATTATAAAC TTCTTCAATG ACTTCAGTGA CCACCTGGCT 
GAGGAGGCCC TATGGGAACT CTCTCTGAAA ATTAAACCCA GGAACATAAC AAGGAGAAAA 
ACAGACCGGG AAGAGAAGAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_008005888.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004079.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCh98999
Clone ID Related Accession (Same CDS sequence)	XM_008005888.1
Accession Version	XM_008005888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGAG TGAAAACGCC 
AGTGAGGAAG CTGGTGAGGG TGAATATGTC AATCTGTATT CCTCTGGCCA GAGCAGCGAG 
GAGCTGGCTC CTTCTCGAGG AGAACCACCG GCTGGGAAAG ACGGACATTC CAGAGATCCC 
TCAGCAGTCA GCAGCATCCC TGGGAAGGAC AGCAGAGACG GCAGTGAGAG GGCCCCAAAG 
TCACCAGATG CTCTGGAGTC GGCTCAGTCG GAGGAGGAAG TGGACGAGCT GTCCCTCATC 
GACCACAACG AAATTATGTC CAGGCTGACG CTCAAGCAGG AGGGTGATGA CGGGCCGGAT 
GTCCGCGGAG GATCTGGTGA CATCTTATTG GTCCATGCTA CTGAGACTGA CAGGAAAGAT 
TTGGTGTTGT ACTGCGAGGC CTTCCTGACC ACCTACAGGA CCTTCATCTC CCCAGAGGAG 
CTCATCAAGA AGCTGCAGTA CAGATACGAG AAATTCTCTC CCTTCGCCGA TACGTTCAAG 
AAGCGCGTCA GCAAGAACAC ATTCTTCGTA CTGGTGCGGG TGGTGGATGA GCTTTGCCTG 
GTGGAATTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAAC TGGTCTTCCG CCTGGTGTGC 
AACGGGGAGC TGAGCCTGGC CCGCGTGCTC CGGAAGAACA TCCTGGACAA GGTGGACCAG 
AAGAAGCTGC TCAGGTGTGC CACCTCTGGC CAGCCCCTGG CGGCCCGGGG GGTAGCAGCC 
AGGCCGGGGA CCTTGCACGA CTTTCACAGC CATGAGATCG CGGAGCAGCT AACTCTGCTG 
GATGCTGAGC TCTTCTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC AAAAGAGCAG 
AATGAGGAAA AGAGCCCCAA CTTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CCATAATCAT GTTACAGGAA AAGGCCCAGG ACAGGGAACG GCTGCTCTTA 
AAGTTCATCA AGATCATGAA GCACTTGCGG AAGCTGAATA ACTTCAACTC CTACTTGGCC 
ATCCTCTCTG CCCTGGACTC AGCGCCCATC CGCAGGCTGG AGTGGCAGAA GCAGACCTCA 
GAGGGCCTGG CCGAGTACTG CACGCTGATT GACAGCTCAT CCTCCTTCCG AGCCTACCGG 
GCCGCCCTCT CGGAGGTGGA ACCGCCGTGC ATCCCGTACC TGGGGCTGAT CCTGCAGGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAC TACATCGATG GGAAAGTGAA CTTCTCCAAG 
CGGTGGCAGC AGTTCAACAT CCTCGACAGC ATGCGGTGCT TCCAGCAGGC GCACTATGAC 
ATCCGGAGGA ACGACGACAT TATAAACTTC TTCAATGACT TCAGTGACCA CCTGGCTGAG 
GAGGCCCTAT GGGAACTCTC TCTGAAAATT AAACCCAGGA ACATAACAAG GAGAAAAACA 
GACCGGGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004079.1
CDS	968..4348
Translation	MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005888.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005888.1

ORF Insert Sequence:

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGAG TGAAAACGCC 
AGTGAGGAAG CTGGTGAGGG TGAATATGTC AATCTGTATT CCTCTGGCCA GAGCAGCGAG 
GAGCTGGCTC CTTCTCGAGG AGAACCACCG GCTGGGAAAG ACGGACATTC CAGAGATCCC 
TCAGCAGTCA GCAGCATCCC TGGGAAGGAC AGCAGAGACG GCAGTGAGAG GGCCCCAAAG 
TCACCAGATG CTCTGGAGTC GGCTCAGTCG GAGGAGGAAG TGGACGAGCT GTCCCTCATC 
GACCACAACG AAATTATGTC CAGGCTGACG CTCAAGCAGG AGGGTGATGA CGGGCCGGAT 
GTCCGCGGAG GATCTGGTGA CATCTTATTG GTCCATGCTA CTGAGACTGA CAGGAAAGAT 
TTGGTGTTGT ACTGCGAGGC CTTCCTGACC ACCTACAGGA CCTTCATCTC CCCAGAGGAG 
CTCATCAAGA AGCTGCAGTA CAGATACGAG AAATTCTCTC CCTTCGCCGA TACGTTCAAG 
AAGCGCGTCA GCAAGAACAC ATTCTTCGTA CTGGTGCGGG TGGTGGATGA GCTTTGCCTG 
GTGGAATTGA CAGAAGAGAT CCTGAAGCTG CTGATGGAAC TGGTCTTCCG CCTGGTGTGC 
AACGGGGAGC TGAGCCTGGC CCGCGTGCTC CGGAAGAACA TCCTGGACAA GGTGGACCAG 
AAGAAGCTGC TCAGGTGTGC CACCTCTGGC CAGCCCCTGG CGGCCCGGGG GGTAGCAGCC 
AGGCCGGGGA CCTTGCACGA CTTTCACAGC CATGAGATCG CGGAGCAGCT AACTCTGCTG 
GATGCTGAGC TCTTCTATAA AATAGAGATT CCTGAGGTTT TGCTTTGGGC AAAAGAGCAG 
AATGAGGAAA AGAGCCCCAA CTTGACCCAG TTCACGGAGC ACTTCAACAA CATGTCCTAC 
TGGGTCCGGT CCATAATCAT GTTACAGGAA AAGGCCCAGG ACAGGGAACG GCTGCTCTTA 
AAGTTCATCA AGATCATGAA GCACTTGCGG AAGCTGAATA ACTTCAACTC CTACTTGGCC 
ATCCTCTCTG CCCTGGACTC AGCGCCCATC CGCAGGCTGG AGTGGCAGAA GCAGACCTCA 
GAGGGCCTGG CCGAGTACTG CACGCTGATT GACAGCTCAT CCTCCTTCCG AGCCTACCGG 
GCCGCCCTCT CGGAGGTGGA ACCGCCGTGC ATCCCGTACC TGGGGCTGAT CCTGCAGGAC 
CTGACCTTCG TTCACCTGGG AAACCCAGAC TACATCGATG GGAAAGTGAA CTTCTCCAAG 
CGGTGGCAGC AGTTCAACAT CCTCGACAGC ATGCGGTGCT TCCAGCAGGC GCACTATGAC 
ATCCGGAGGA ACGACGACAT TATAAACTTC TTCAATGACT TCAGTGACCA CCTGGCTGAG 
GAGGCCCTAT GGGAACTCTC TCTGAAAATT AAACCCAGGA ACATAACAAG GAGAAAAACA 
GACCGGGAAG AGAAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_008005889.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004080.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCh99000
Clone ID Related Accession (Same CDS sequence)	XM_008005889.1
Accession Version	XM_008005889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGGA ACCACCGGCT 
GGGAAAGACG GACATTCCAG AGATCCCTCA GCAGTCAGCA GCATCCCTGG GAAGGACAGC 
AGAGACGGCA GTGAGAGGGC CCCAAAGTCA CCAGATGCTC TGGAGTCGGC TCAGTCGGAG 
GAGGAAGTGG ACGAGCTGTC CCTCATCGAC CACAACGAAA TTATGTCCAG GCTGACGCTC 
AAGCAGGAGG GTGATGACGG GCCGGATGTC CGCGGAGGAT CTGGTGACAT CTTATTGGTC 
CATGCTACTG AGACTGACAG GAAAGATTTG GTGTTGTACT GCGAGGCCTT CCTGACCACC 
TACAGGACCT TCATCTCCCC AGAGGAGCTC ATCAAGAAGC TGCAGTACAG ATACGAGAAA 
TTCTCTCCCT TCGCCGATAC GTTCAAGAAG CGCGTCAGCA AGAACACATT CTTCGTACTG 
GTGCGGGTGG TGGATGAGCT TTGCCTGGTG GAATTGACAG AAGAGATCCT GAAGCTGCTG 
ATGGAACTGG TCTTCCGCCT GGTGTGCAAC GGGGAGCTGA GCCTGGCCCG CGTGCTCCGG 
AAGAACATCC TGGACAAGGT GGACCAGAAG AAGCTGCTCA GGTGTGCCAC CTCTGGCCAG 
CCCCTGGCGG CCCGGGGGGT AGCAGCCAGG CCGGGGACCT TGCACGACTT TCACAGCCAT 
GAGATCGCGG AGCAGCTAAC TCTGCTGGAT GCTGAGCTCT TCTATAAAAT AGAGATTCCT 
GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT GAGGAAAAGA GCCCCAACTT GACCCAGTTC 
ACGGAGCACT TCAACAACAT GTCCTACTGG GTCCGGTCCA TAATCATGTT ACAGGAAAAG 
GCCCAGGACA GGGAACGGCT GCTCTTAAAG TTCATCAAGA TCATGAAGCA CTTGCGGAAG 
CTGAATAACT TCAACTCCTA CTTGGCCATC CTCTCTGCCC TGGACTCAGC GCCCATCCGC 
AGGCTGGAGT GGCAGAAGCA GACCTCAGAG GGCCTGGCCG AGTACTGCAC GCTGATTGAC 
AGCTCATCCT CCTTCCGAGC CTACCGGGCC GCCCTCTCGG AGGTGGAACC GCCGTGCATC 
CCGTACCTGG GGCTGATCCT GCAGGACCTG ACCTTCGTTC ACCTGGGAAA CCCAGACTAC 
ATCGATGGGA AAGTGAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT CGACAGCATG 
CGGTGCTTCC AGCAGGCGCA CTATGACATC CGGAGGAACG ACGACATTAT AAACTTCTTC 
AATGACTTCA GTGACCACCT GGCTGAGGAG GCCCTATGGG AACTCTCTCT GAAAATTAAA 
CCCAGGAACA TAACAAGGAG AAAAACAGAC CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004080.1
CDS	967..4254
Translation	MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005889.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005889.1

ORF Insert Sequence:

ATGGGCAATG CTATTGAAAA ACAGAAACCC TTGAAACGAA GTCATCTGTA CCCCTGGAAA 
CAAGACTCTC AGCGTTCTCA TCTCTCTTCC TTCACCATGA AGCTGATGGA CAAATTCCAC 
TCACCCAAAA TCAAGAGAAC GCCATCAAAG AAGGGAAAAC CAGCTGAGGT GTCCGTAAAG 
ATTCCAGAGA AGCCTGTGAA CAAAGAGGCA ACAGACAGAT TTCTACCAGA GGGCTACCCT 
CTCCCCTTGG ATCTGGAGCA GCAGGCAGTA GAATTTATGT CCACCAGTGC TGTGGCTTCC 
AGGTCTCAAA GGCAGAAGAA CCTGAGCTGG CTGGAGGAGA AAGAGAAGGA AGTTGTCAGT 
GCCCTGCGCT ACTTTAAGAC CATTGTGGAC AAAATGGCAA TTGATAAGAA GGTACTGGAG 
ATGCTTCCAG GGTCAGCCAG CAAGGTGCTG GAGGCCATCT TACCCCTGGT GCAGAACGAT 
CCTCGAATTC AGCACAGCTC AGCCCTGTCT TCCTGCTATA GCCGAGTGTA CCAAAGTCTC 
GCCAACCTCA TTCGCTGGTC TGACCAAGTG ATGCTGGAAG GTGTGAACTC AGAAGACAAG 
GAGATGGTGA CGACGGTGAA GGGGGTCATC AAGGCCGTGC TGGATGGAGT GAAGGAGCTG 
GTCAGGCTCA CCGTCGAGAA GCAGGGACGT CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT 
TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA GAGCTCCCCC TGACAGACCG CGAGGTAGAA 
ATCCTAAACA AGACGACCGG GATGTCACAG TCAACTGAGC TCCTCCCAGA CGCCACAGAT 
GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG CCTGGCATTC GGGTGGTTGA TAATAGTCCT 
CCACCAGCAT TGCCACCCAA GAAAAGACAG TCGGCACCGT CCCCTACCCG AGTGGCTGTG 
GTGGCCCCCA TGAGCCGAGC CACCAGTGGC TCCAGTTTGC CTGTTGGAAT CAATAGGCAG 
GATTTTGACG TTGACTGTTA TGCACAGAGG CGACTGTCAG GAGGCAGCCA CTCATATGGT 
GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC AGCATAGGCA AGCTCAGCAA GTCAGACGAG 
CAGCTGTCCT CTCTGGACAG GGACAGTGGG CAGTGCTCCC GGAACACAAG CTGTGAAACA 
CTAGACCACT ATGATCCCGA CTATGAATTC CTCCAGCAAG ACCTCTCTAA CGCAGACCAG 
ATACCTCAGC AGACGGCCTG GAACCTTAGC CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG 
TCTCCATTTG TTGGCCATCC TTTCCAGCTG CCTCTTGGCA GCCATCCCCA GCCAGACGGA 
CCTCTGGCCC CAGGGCAGCA GACAGTTACG CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG 
AGTGCAGCCT CCCAGACGGC GGACAGCTCT GGCTGCAGGG TGTCCTACGA GCGGCATCCC 
TCGCAGTACG ACAACATCTC TGGGGAGGAC CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC 
CCCTACGCGC CCTTTGCTGC TATTCTCCCC TTTCAGCATG GAGGTTCCTC AGCCCCTGTC 
GAATTTGTGG GTGATTTTAC TGCTCCTGAA TCAACGGGTG ACCCAGAAAA ACCACCTCCT 
CTACCAGAGA AGAAAAACAA ACACATGCTG GCCTACATGC AGCTGCTGGA GGACTACTCG 
GAGCCACAGC CCTCCATGTT CTACCAGACG CCGCAGAACG AGCACATCTA CCAGCAGAAG 
AACAAGCTCC TCATGGAGGT ATACGGCTTC AGCGACTCCT TCAGCGGGGT GGACTCTGTG 
CAGGAGCTGG CCCCGCCGCC CGCCCTACCC CCCAAGCAGC GGCAGCTGGA ACCACCGGCT 
GGGAAAGACG GACATTCCAG AGATCCCTCA GCAGTCAGCA GCATCCCTGG GAAGGACAGC 
AGAGACGGCA GTGAGAGGGC CCCAAAGTCA CCAGATGCTC TGGAGTCGGC TCAGTCGGAG 
GAGGAAGTGG ACGAGCTGTC CCTCATCGAC CACAACGAAA TTATGTCCAG GCTGACGCTC 
AAGCAGGAGG GTGATGACGG GCCGGATGTC CGCGGAGGAT CTGGTGACAT CTTATTGGTC 
CATGCTACTG AGACTGACAG GAAAGATTTG GTGTTGTACT GCGAGGCCTT CCTGACCACC 
TACAGGACCT TCATCTCCCC AGAGGAGCTC ATCAAGAAGC TGCAGTACAG ATACGAGAAA 
TTCTCTCCCT TCGCCGATAC GTTCAAGAAG CGCGTCAGCA AGAACACATT CTTCGTACTG 
GTGCGGGTGG TGGATGAGCT TTGCCTGGTG GAATTGACAG AAGAGATCCT GAAGCTGCTG 
ATGGAACTGG TCTTCCGCCT GGTGTGCAAC GGGGAGCTGA GCCTGGCCCG CGTGCTCCGG 
AAGAACATCC TGGACAAGGT GGACCAGAAG AAGCTGCTCA GGTGTGCCAC CTCTGGCCAG 
CCCCTGGCGG CCCGGGGGGT AGCAGCCAGG CCGGGGACCT TGCACGACTT TCACAGCCAT 
GAGATCGCGG AGCAGCTAAC TCTGCTGGAT GCTGAGCTCT TCTATAAAAT AGAGATTCCT 
GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT GAGGAAAAGA GCCCCAACTT GACCCAGTTC 
ACGGAGCACT TCAACAACAT GTCCTACTGG GTCCGGTCCA TAATCATGTT ACAGGAAAAG 
GCCCAGGACA GGGAACGGCT GCTCTTAAAG TTCATCAAGA TCATGAAGCA CTTGCGGAAG 
CTGAATAACT TCAACTCCTA CTTGGCCATC CTCTCTGCCC TGGACTCAGC GCCCATCCGC 
AGGCTGGAGT GGCAGAAGCA GACCTCAGAG GGCCTGGCCG AGTACTGCAC GCTGATTGAC 
AGCTCATCCT CCTTCCGAGC CTACCGGGCC GCCCTCTCGG AGGTGGAACC GCCGTGCATC 
CCGTACCTGG GGCTGATCCT GCAGGACCTG ACCTTCGTTC ACCTGGGAAA CCCAGACTAC 
ATCGATGGGA AAGTGAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT CGACAGCATG 
CGGTGCTTCC AGCAGGCGCA CTATGACATC CGGAGGAACG ACGACATTAT AAACTTCTTC 
AATGACTTCA GTGACCACCT GGCTGAGGAG GCCCTATGGG AACTCTCTCT GAAAATTAAA 
CCCAGGAACA TAACAAGGAG AAAAACAGAC CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_008005890.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004081.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X11, mRNA.

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CloneID	OCh99001
Clone ID Related Accession (Same CDS sequence)	XM_008005890.1
Accession Version	XM_008005890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTCTC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
CTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCAATTG ATAAGAAGGT ACTGGAGATG 
CTTCCAGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAACGATCCT 
CGAATTCAGC ACAGCTCAGC CCTGTCTTCC TGCTATAGCC GAGTGTACCA AAGTCTCGCC 
AACCTCATTC GCTGGTCTGA CCAAGTGATG CTGGAAGGTG TGAACTCAGA AGACAAGGAG 
ATGGTGACGA CGGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGGCTCACCG TCGAGAAGCA GGGACGTCCG TCTCCAACAA GCCCCGTGAA GCCCAGTTCC 
CCTGCTGGCA AGCCTGATGG CCCAGCAGAG CTCCCCCTGA CAGACCGCGA GGTAGAAATC 
CTAAACAAGA CGACCGGGAT GTCACAGTCA ACTGAGCTCC TCCCAGACGC CACAGATGAA 
GAGGTCGCGC CCCCCAAGCC CCCTCTGCCT GGCATTCGGG TGGTTGATAA TAGTCCTCCA 
CCAGCATTGC CACCCAAGAA AAGACAGTCG GCACCGTCCC CTACCCGAGT GGCTGTGGTG 
GCCCCCATGA GCCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAT 
TTTGACGTTG ACTGTTATGC ACAGAGGCGA CTGTCAGGAG GCAGCCACTC ATATGGTGGA 
GAGTCGCCCC GCCTCTCCCC CTGCAGCAGC ATAGGCAAGC TCAGCAAGTC AGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGTGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GACCACTATG ATCCCGACTA TGAATTCCTC CAGCAAGACC TCTCTAACGC AGACCAGATA 
CCTCAGCAGA CGGCCTGGAA CCTTAGCCCG TTGCCAGAGT CTTTGGGGGA GTCTGGGTCT 
CCATTTGTTG GCCATCCTTT CCAGCTGCCT CTTGGCAGCC ATCCCCAGCC AGACGGACCT 
CTGGCCCCAG GGCAGCAGAC AGTTACGCCG CCTGCTCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACGGCGGA CAGCTCTGGC TGCAGGGTGT CCTACGAGCG GCATCCCTCG 
CAGTACGACA ACATCTCTGG GGAGGACCTG CAGAGCACGG CCCCAATCCA GTCCGTCCCC 
TACGCGCCCT TTGCTGCTAT TCTCCCCTTT CAGCATGGAG GTTCCTCAGC CCCTGTCGAA 
TTTGTGGGTG ATTTTACTGC TCCTGAATCA ACGGGTGACC CAGAAAAACC ACCTCCTCTA 
CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTGGAGGA CTACTCGGAG 
CCACAGCCCT CCATGTTCTA CCAGACGCCG CAGAACGAGC ACATCTACCA GCAGAAGAAC 
AAGCTCCTCA TGGAGGTATA CGGCTTCAGC GACTCCTTCA GCGGGGTGGA CTCTGTGCAG 
GAGCTGGCCC CGCCGCCCGC CCTACCCCCC AAGCAGCGGC AGCTGGAACC ACCGGCTGGG 
AAAGACGGAC ATTCCAGAGA TCCCTCAGCA GTCAGCAGCA TCCCTGGGAA GGACAGCAGA 
GACGGCAGTG AGAGGGCCCC AAAGTCACCA GATGCTCTGG AGTCGGCTCA GTCGGAGGAG 
GAAGTGGACG AGCTGTCCCT CATCGACCAC AACGAAATTA TGTCCAGGCT GACGCTCAAG 
CAGGAGGGTG ATGACGGGCC GGATGTCCGC GGAGGATCTG GTGACATCTT ATTGGTCCAT 
GCTACTGAGA CTGACAGGAA AGATTTGGTG TTGTACTGCG AGGCCTTCCT GACCACCTAC 
AGGACCTTCA TCTCCCCAGA GGAGCTCATC AAGAAGCTGC AGTACAGATA CGAGAAATTC 
TCTCCCTTCG CCGATACGTT CAAGAAGCGC GTCAGCAAGA ACACATTCTT CGTACTGGTG 
CGGGTGGTGG ATGAGCTTTG CCTGGTGGAA TTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAACTGGTCT TCCGCCTGGT GTGCAACGGG GAGCTGAGCC TGGCCCGCGT GCTCCGGAAG 
AACATCCTGG ACAAGGTGGA CCAGAAGAAG CTGCTCAGGT GTGCCACCTC TGGCCAGCCC 
CTGGCGGCCC GGGGGGTAGC AGCCAGGCCG GGGACCTTGC ACGACTTTCA CAGCCATGAG 
ATCGCGGAGC AGCTAACTCT GCTGGATGCT GAGCTCTTCT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAG GAAAAGAGCC CCAACTTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCCATAA TCATGTTACA GGAAAAGGCC 
CAGGACAGGG AACGGCTGCT CTTAAAGTTC ATCAAGATCA TGAAGCACTT GCGGAAGCTG 
AATAACTTCA ACTCCTACTT GGCCATCCTC TCTGCCCTGG ACTCAGCGCC CATCCGCAGG 
CTGGAGTGGC AGAAGCAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATTGACAGC 
TCATCCTCCT TCCGAGCCTA CCGGGCCGCC CTCTCGGAGG TGGAACCGCC GTGCATCCCG 
TACCTGGGGC TGATCCTGCA GGACCTGACC TTCGTTCACC TGGGAAACCC AGACTACATC 
GATGGGAAAG TGAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTCGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA TGACATCCGG AGGAACGACG ACATTATAAA CTTCTTCAAT 
GACTTCAGTG ACCACCTGGC TGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATTAAACCC 
AGGAACATAA CAAGGAGAAA AACAGACCGG GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004081.1
CDS	90..3374
Translation	MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005890.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005890.1

ORF Insert Sequence:

ATGAGCGGCG GCCTCGGCCT CCGGCGCAGC CCGGAAATGT CCGGCAAGAT CGAGAAAGCA 
GACTCTCAGC GTTCTCATCT CTCTTCCTTC ACCATGAAGC TGATGGACAA ATTCCACTCA 
CCCAAAATCA AGAGAACGCC ATCAAAGAAG GGAAAACCAG CTGAGGTGTC CGTAAAGATT 
CCAGAGAAGC CTGTGAACAA AGAGGCAACA GACAGATTTC TACCAGAGGG CTACCCTCTC 
CCCTTGGATC TGGAGCAGCA GGCAGTAGAA TTTATGTCCA CCAGTGCTGT GGCTTCCAGG 
TCTCAAAGGC AGAAGAACCT GAGCTGGCTG GAGGAGAAAG AGAAGGAAGT TGTCAGTGCC 
CTGCGCTACT TTAAGACCAT TGTGGACAAA ATGGCAATTG ATAAGAAGGT ACTGGAGATG 
CTTCCAGGGT CAGCCAGCAA GGTGCTGGAG GCCATCTTAC CCCTGGTGCA GAACGATCCT 
CGAATTCAGC ACAGCTCAGC CCTGTCTTCC TGCTATAGCC GAGTGTACCA AAGTCTCGCC 
AACCTCATTC GCTGGTCTGA CCAAGTGATG CTGGAAGGTG TGAACTCAGA AGACAAGGAG 
ATGGTGACGA CGGTGAAGGG GGTCATCAAG GCCGTGCTGG ATGGAGTGAA GGAGCTGGTC 
AGGCTCACCG TCGAGAAGCA GGGACGTCCG TCTCCAACAA GCCCCGTGAA GCCCAGTTCC 
CCTGCTGGCA AGCCTGATGG CCCAGCAGAG CTCCCCCTGA CAGACCGCGA GGTAGAAATC 
CTAAACAAGA CGACCGGGAT GTCACAGTCA ACTGAGCTCC TCCCAGACGC CACAGATGAA 
GAGGTCGCGC CCCCCAAGCC CCCTCTGCCT GGCATTCGGG TGGTTGATAA TAGTCCTCCA 
CCAGCATTGC CACCCAAGAA AAGACAGTCG GCACCGTCCC CTACCCGAGT GGCTGTGGTG 
GCCCCCATGA GCCGAGCCAC CAGTGGCTCC AGTTTGCCTG TTGGAATCAA TAGGCAGGAT 
TTTGACGTTG ACTGTTATGC ACAGAGGCGA CTGTCAGGAG GCAGCCACTC ATATGGTGGA 
GAGTCGCCCC GCCTCTCCCC CTGCAGCAGC ATAGGCAAGC TCAGCAAGTC AGACGAGCAG 
CTGTCCTCTC TGGACAGGGA CAGTGGGCAG TGCTCCCGGA ACACAAGCTG TGAAACACTA 
GACCACTATG ATCCCGACTA TGAATTCCTC CAGCAAGACC TCTCTAACGC AGACCAGATA 
CCTCAGCAGA CGGCCTGGAA CCTTAGCCCG TTGCCAGAGT CTTTGGGGGA GTCTGGGTCT 
CCATTTGTTG GCCATCCTTT CCAGCTGCCT CTTGGCAGCC ATCCCCAGCC AGACGGACCT 
CTGGCCCCAG GGCAGCAGAC AGTTACGCCG CCTGCTCTGC CCGAGAAGAA GCGCAGGAGT 
GCAGCCTCCC AGACGGCGGA CAGCTCTGGC TGCAGGGTGT CCTACGAGCG GCATCCCTCG 
CAGTACGACA ACATCTCTGG GGAGGACCTG CAGAGCACGG CCCCAATCCA GTCCGTCCCC 
TACGCGCCCT TTGCTGCTAT TCTCCCCTTT CAGCATGGAG GTTCCTCAGC CCCTGTCGAA 
TTTGTGGGTG ATTTTACTGC TCCTGAATCA ACGGGTGACC CAGAAAAACC ACCTCCTCTA 
CCAGAGAAGA AAAACAAACA CATGCTGGCC TACATGCAGC TGCTGGAGGA CTACTCGGAG 
CCACAGCCCT CCATGTTCTA CCAGACGCCG CAGAACGAGC ACATCTACCA GCAGAAGAAC 
AAGCTCCTCA TGGAGGTATA CGGCTTCAGC GACTCCTTCA GCGGGGTGGA CTCTGTGCAG 
GAGCTGGCCC CGCCGCCCGC CCTACCCCCC AAGCAGCGGC AGCTGGAACC ACCGGCTGGG 
AAAGACGGAC ATTCCAGAGA TCCCTCAGCA GTCAGCAGCA TCCCTGGGAA GGACAGCAGA 
GACGGCAGTG AGAGGGCCCC AAAGTCACCA GATGCTCTGG AGTCGGCTCA GTCGGAGGAG 
GAAGTGGACG AGCTGTCCCT CATCGACCAC AACGAAATTA TGTCCAGGCT GACGCTCAAG 
CAGGAGGGTG ATGACGGGCC GGATGTCCGC GGAGGATCTG GTGACATCTT ATTGGTCCAT 
GCTACTGAGA CTGACAGGAA AGATTTGGTG TTGTACTGCG AGGCCTTCCT GACCACCTAC 
AGGACCTTCA TCTCCCCAGA GGAGCTCATC AAGAAGCTGC AGTACAGATA CGAGAAATTC 
TCTCCCTTCG CCGATACGTT CAAGAAGCGC GTCAGCAAGA ACACATTCTT CGTACTGGTG 
CGGGTGGTGG ATGAGCTTTG CCTGGTGGAA TTGACAGAAG AGATCCTGAA GCTGCTGATG 
GAACTGGTCT TCCGCCTGGT GTGCAACGGG GAGCTGAGCC TGGCCCGCGT GCTCCGGAAG 
AACATCCTGG ACAAGGTGGA CCAGAAGAAG CTGCTCAGGT GTGCCACCTC TGGCCAGCCC 
CTGGCGGCCC GGGGGGTAGC AGCCAGGCCG GGGACCTTGC ACGACTTTCA CAGCCATGAG 
ATCGCGGAGC AGCTAACTCT GCTGGATGCT GAGCTCTTCT ATAAAATAGA GATTCCTGAG 
GTTTTGCTTT GGGCAAAAGA GCAGAATGAG GAAAAGAGCC CCAACTTGAC CCAGTTCACG 
GAGCACTTCA ACAACATGTC CTACTGGGTC CGGTCCATAA TCATGTTACA GGAAAAGGCC 
CAGGACAGGG AACGGCTGCT CTTAAAGTTC ATCAAGATCA TGAAGCACTT GCGGAAGCTG 
AATAACTTCA ACTCCTACTT GGCCATCCTC TCTGCCCTGG ACTCAGCGCC CATCCGCAGG 
CTGGAGTGGC AGAAGCAGAC CTCAGAGGGC CTGGCCGAGT ACTGCACGCT GATTGACAGC 
TCATCCTCCT TCCGAGCCTA CCGGGCCGCC CTCTCGGAGG TGGAACCGCC GTGCATCCCG 
TACCTGGGGC TGATCCTGCA GGACCTGACC TTCGTTCACC TGGGAAACCC AGACTACATC 
GATGGGAAAG TGAACTTCTC CAAGCGGTGG CAGCAGTTCA ACATCCTCGA CAGCATGCGG 
TGCTTCCAGC AGGCGCACTA TGACATCCGG AGGAACGACG ACATTATAAA CTTCTTCAAT 
GACTTCAGTG ACCACCTGGC TGAGGAGGCC CTATGGGAAC TCTCTCTGAA AATTAAACCC 
AGGAACATAA CAAGGAGAAA AACAGACCGG GAAGAGAAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF1 ( XM_008005891.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008004082.1 Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X12, mRNA.

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CloneID	OCh99002
Clone ID Related Accession (Same CDS sequence)	XM_008005891.1
Accession Version	XM_008005891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	rap guanine nucleotide exchange factor 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAAG GTGTGAACTC AGAAGACAAG GAGATGGTGA CGACGGTGAA GGGGGTCATC 
AAGGCCGTGC TGGATGGAGT GAAGGAGCTG GTCAGGCTCA CCGTCGAGAA GCAGGGACGT 
CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA 
GAGCTCCCCC TGACAGACCG CGAGGTAGAA ATCCTAAACA AGACGACCGG GATGTCACAG 
TCAACTGAGC TCCTCCCAGA CGCCACAGAT GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG 
CCTGGCATTC GGGTGGTTGA TAATAGTCCT CCACCAGCAT TGCCACCCAA GAAAAGACAG 
TCGGCACCGT CCCCTACCCG AGTGGCTGTG GTGGCCCCCA TGAGCCGAGC CACCAGTGGC 
TCCAGTTTGC CTGTTGGAAT CAATAGGCAG GATTTTGACG TTGACTGTTA TGCACAGAGG 
CGACTGTCAG GAGGCAGCCA CTCATATGGT GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC 
AGCATAGGCA AGCTCAGCAA GTCAGACGAG CAGCTGTCCT CTCTGGACAG GGACAGTGGG 
CAGTGCTCCC GGAACACAAG CTGTGAAACA CTAGACCACT ATGATCCCGA CTATGAATTC 
CTCCAGCAAG ACCTCTCTAA CGCAGACCAG ATACCTCAGC AGACGGCCTG GAACCTTAGC 
CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG TCTCCATTTG TTGGCCATCC TTTCCAGCTG 
CCTCTTGGCA GCCATCCCCA GCCAGACGGA CCTCTGGCCC CAGGGCAGCA GACAGTTACG 
CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG AGTGCAGCCT CCCAGACGGC GGACAGCTCT 
GGCTGCAGGG TGTCCTACGA GCGGCATCCC TCGCAGTACG ACAACATCTC TGGGGAGGAC 
CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC CCCTACGCGC CCTTTGCTGC TATTCTCCCC 
TTTCAGCATG GAGGTTCCTC AGCCCCTGTC GAATTTGTGG GTGATTTTAC TGCTCCTGAA 
TCAACGGGTG ACCCAGAAAA ACCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG 
GCCTACATGC AGCTGCTGGA GGACTACTCG GAGCCACAGC CCTCCATGTT CTACCAGACG 
CCGCAGAACG AGCACATCTA CCAGCAGAAG AACAAGCTCC TCATGGAGGT ATACGGCTTC 
AGCGACTCCT TCAGCGGGGT GGACTCTGTG CAGGAGCTGG CCCCGCCGCC CGCCCTACCC 
CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC 
TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCGC TCAGGGCCTC 
AGTGTGTCCG TCTCTAACTC CTTTCTCAGC CGGCATGGCA GCTTGCCTGT GCCCTCGTAT 
AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACCACCAAGC TTCCGTTCCG 
CCTTTCCTTC CGCCTACCTC CTCTTCCTCT CCACATTTCC CACCTGCCCA CCAGTCTCAG 
AGCTCTGACT TAGCCGTGCC AGCCATGGCC GGTCCACCTC CCAGCACCGT GGACGGGCCT 
CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT GGGAATACTG TCTGCCTTCC TTCCGAAACC 
TCTTTCACTG ACTCGAGTGA AAACGCCAGT GAGGAAGCTG GTGAGGGTGA ATATGTCAAT 
CTGTATTCCT CTGGCCAGAG CAGCGAGGAG CTGGCTCCTT CTCGAGGAGA ACCACCGGCT 
GGGAAAGACG GACATTCCAG AGATCCCTCA GCAGTCAGCA GCATCCCTGG GAAGGACAGC 
AGAGACGGCA GTGAGAGGGC CCCAAAGTCA CCAGATGCTC TGGAGTCGGC TCAGTCGGAG 
GAGGAAGTGG ACGAGCTGTC CCTCATCGAC CACAACGAAA TTATGTCCAG GCTGACGCTC 
AAGCAGGAGG GTGATGACGG GCCGGATGTC CGCGGAGGAT CTGGTGACAT CTTATTGGTC 
CATGCTACTG AGACTGACAG GAAAGATTTG GTGTTGTACT GCGAGGCCTT CCTGACCACC 
TACAGGACCT TCATCTCCCC AGAGGAGCTC ATCAAGAAGC TGCAGTACAG ATACGAGAAA 
TTCTCTCCCT TCGCCGATAC GTTCAAGAAG CGCGTCAGCA AGAACACATT CTTCGTACTG 
GTGCGGGTGG TGGATGAGCT TTGCCTGGTG GAATTGACAG AAGAGATCCT GAAGCTGCTG 
ATGGAACTGG TCTTCCGCCT GGTGTGCAAC GGGGAGCTGA GCCTGGCCCG CGTGCTCCGG 
AAGAACATCC TGGACAAGGT GGACCAGAAG AAGCTGCTCA GGTGTGCCAC CTCTGGCCAG 
CCCCTGGCGG CCCGGGGGGT AGCAGCCAGG CCGGGGACCT TGCACGACTT TCACAGCCAT 
GAGATCGCGG AGCAGCTAAC TCTGCTGGAT GCTGAGCTCT TCTATAAAAT AGAGATTCCT 
GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT GAGGAAAAGA GCCCCAACTT GACCCAGTTC 
ACGGAGCACT TCAACAACAT GTCCTACTGG GTCCGGTCCA TAATCATGTT ACAGGAAAAG 
GCCCAGGACA GGGAACGGCT GCTCTTAAAG TTCATCAAGA TCATGAAGCA CTTGCGGAAG 
CTGAATAACT TCAACTCCTA CTTGGCCATC CTCTCTGCCC TGGACTCAGC GCCCATCCGC 
AGGCTGGAGT GGCAGAAGCA GACCTCAGAG GGCCTGGCCG AGTACTGCAC GCTGATTGAC 
AGCTCATCCT CCTTCCGAGC CTACCGGGCC GCCCTCTCGG AGGTGGAACC GCCGTGCATC 
CCGTACCTGG GGCTGATCCT GCAGGACCTG ACCTTCGTTC ACCTGGGAAA CCCAGACTAC 
ATCGATGGGA AAGTGAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT CGACAGCATG 
CGGTGCTTCC AGCAGGCGCA CTATGACATC CGGAGGAACG ACGACATTAT AAACTTCTTC 
AATGACTTCA GTGACCACCT GGCTGAGGAG GCCCTATGGG AACTCTCTCT GAAAATTAAA 
CCCAGGAACA TAACAAGGAG AAAAACAGAC CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008004082.1
CDS	162..3389
Translation	MLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTVEKQGRPSPTSPVKPSSPAGKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFVGHPFQLPLGSHPQPDGPLAPGQQTVTPPALPEKKRRSAASQTADSSGCRVSYERHPSQYDNISGEDLQSTAPIQSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLQASCAASSFSSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGSLPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPAMAGPPPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEELAPSRGEPPAGKDGHSRDPSAVSSIPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSGQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT

Target ORF information:

RefSeq Version	XM_008005891.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005891.1

ORF Insert Sequence:

ATGCTGGAAG GTGTGAACTC AGAAGACAAG GAGATGGTGA CGACGGTGAA GGGGGTCATC 
AAGGCCGTGC TGGATGGAGT GAAGGAGCTG GTCAGGCTCA CCGTCGAGAA GCAGGGACGT 
CCGTCTCCAA CAAGCCCCGT GAAGCCCAGT TCCCCTGCTG GCAAGCCTGA TGGCCCAGCA 
GAGCTCCCCC TGACAGACCG CGAGGTAGAA ATCCTAAACA AGACGACCGG GATGTCACAG 
TCAACTGAGC TCCTCCCAGA CGCCACAGAT GAAGAGGTCG CGCCCCCCAA GCCCCCTCTG 
CCTGGCATTC GGGTGGTTGA TAATAGTCCT CCACCAGCAT TGCCACCCAA GAAAAGACAG 
TCGGCACCGT CCCCTACCCG AGTGGCTGTG GTGGCCCCCA TGAGCCGAGC CACCAGTGGC 
TCCAGTTTGC CTGTTGGAAT CAATAGGCAG GATTTTGACG TTGACTGTTA TGCACAGAGG 
CGACTGTCAG GAGGCAGCCA CTCATATGGT GGAGAGTCGC CCCGCCTCTC CCCCTGCAGC 
AGCATAGGCA AGCTCAGCAA GTCAGACGAG CAGCTGTCCT CTCTGGACAG GGACAGTGGG 
CAGTGCTCCC GGAACACAAG CTGTGAAACA CTAGACCACT ATGATCCCGA CTATGAATTC 
CTCCAGCAAG ACCTCTCTAA CGCAGACCAG ATACCTCAGC AGACGGCCTG GAACCTTAGC 
CCGTTGCCAG AGTCTTTGGG GGAGTCTGGG TCTCCATTTG TTGGCCATCC TTTCCAGCTG 
CCTCTTGGCA GCCATCCCCA GCCAGACGGA CCTCTGGCCC CAGGGCAGCA GACAGTTACG 
CCGCCTGCTC TGCCCGAGAA GAAGCGCAGG AGTGCAGCCT CCCAGACGGC GGACAGCTCT 
GGCTGCAGGG TGTCCTACGA GCGGCATCCC TCGCAGTACG ACAACATCTC TGGGGAGGAC 
CTGCAGAGCA CGGCCCCAAT CCAGTCCGTC CCCTACGCGC CCTTTGCTGC TATTCTCCCC 
TTTCAGCATG GAGGTTCCTC AGCCCCTGTC GAATTTGTGG GTGATTTTAC TGCTCCTGAA 
TCAACGGGTG ACCCAGAAAA ACCACCTCCT CTACCAGAGA AGAAAAACAA ACACATGCTG 
GCCTACATGC AGCTGCTGGA GGACTACTCG GAGCCACAGC CCTCCATGTT CTACCAGACG 
CCGCAGAACG AGCACATCTA CCAGCAGAAG AACAAGCTCC TCATGGAGGT ATACGGCTTC 
AGCGACTCCT TCAGCGGGGT GGACTCTGTG CAGGAGCTGG CCCCGCCGCC CGCCCTACCC 
CCCAAGCAGC GGCAGCTGCA GGCCTCCTGT GCTGCTTCTT CCTTCTCCTC TGTCTCCTAC 
TGTGTCCAGC AAACTAAAGT GGCCTTCACC CCCGAGGACG GCAGTGCCGC TCAGGGCCTC 
AGTGTGTCCG TCTCTAACTC CTTTCTCAGC CGGCATGGCA GCTTGCCTGT GCCCTCGTAT 
AAGTCTGTGT TTAGGTCCTA CTCCCAGGAT TTCGTGCCTC ACCACCAAGC TTCCGTTCCG 
CCTTTCCTTC CGCCTACCTC CTCTTCCTCT CCACATTTCC CACCTGCCCA CCAGTCTCAG 
AGCTCTGACT TAGCCGTGCC AGCCATGGCC GGTCCACCTC CCAGCACCGT GGACGGGCCT 
CTCTCGGCTT CTCAGGAGAG CAGCTTTCAT GGGAATACTG TCTGCCTTCC TTCCGAAACC 
TCTTTCACTG ACTCGAGTGA AAACGCCAGT GAGGAAGCTG GTGAGGGTGA ATATGTCAAT 
CTGTATTCCT CTGGCCAGAG CAGCGAGGAG CTGGCTCCTT CTCGAGGAGA ACCACCGGCT 
GGGAAAGACG GACATTCCAG AGATCCCTCA GCAGTCAGCA GCATCCCTGG GAAGGACAGC 
AGAGACGGCA GTGAGAGGGC CCCAAAGTCA CCAGATGCTC TGGAGTCGGC TCAGTCGGAG 
GAGGAAGTGG ACGAGCTGTC CCTCATCGAC CACAACGAAA TTATGTCCAG GCTGACGCTC 
AAGCAGGAGG GTGATGACGG GCCGGATGTC CGCGGAGGAT CTGGTGACAT CTTATTGGTC 
CATGCTACTG AGACTGACAG GAAAGATTTG GTGTTGTACT GCGAGGCCTT CCTGACCACC 
TACAGGACCT TCATCTCCCC AGAGGAGCTC ATCAAGAAGC TGCAGTACAG ATACGAGAAA 
TTCTCTCCCT TCGCCGATAC GTTCAAGAAG CGCGTCAGCA AGAACACATT CTTCGTACTG 
GTGCGGGTGG TGGATGAGCT TTGCCTGGTG GAATTGACAG AAGAGATCCT GAAGCTGCTG 
ATGGAACTGG TCTTCCGCCT GGTGTGCAAC GGGGAGCTGA GCCTGGCCCG CGTGCTCCGG 
AAGAACATCC TGGACAAGGT GGACCAGAAG AAGCTGCTCA GGTGTGCCAC CTCTGGCCAG 
CCCCTGGCGG CCCGGGGGGT AGCAGCCAGG CCGGGGACCT TGCACGACTT TCACAGCCAT 
GAGATCGCGG AGCAGCTAAC TCTGCTGGAT GCTGAGCTCT TCTATAAAAT AGAGATTCCT 
GAGGTTTTGC TTTGGGCAAA AGAGCAGAAT GAGGAAAAGA GCCCCAACTT GACCCAGTTC 
ACGGAGCACT TCAACAACAT GTCCTACTGG GTCCGGTCCA TAATCATGTT ACAGGAAAAG 
GCCCAGGACA GGGAACGGCT GCTCTTAAAG TTCATCAAGA TCATGAAGCA CTTGCGGAAG 
CTGAATAACT TCAACTCCTA CTTGGCCATC CTCTCTGCCC TGGACTCAGC GCCCATCCGC 
AGGCTGGAGT GGCAGAAGCA GACCTCAGAG GGCCTGGCCG AGTACTGCAC GCTGATTGAC 
AGCTCATCCT CCTTCCGAGC CTACCGGGCC GCCCTCTCGG AGGTGGAACC GCCGTGCATC 
CCGTACCTGG GGCTGATCCT GCAGGACCTG ACCTTCGTTC ACCTGGGAAA CCCAGACTAC 
ATCGATGGGA AAGTGAACTT CTCCAAGCGG TGGCAGCAGT TCAACATCCT CGACAGCATG 
CGGTGCTTCC AGCAGGCGCA CTATGACATC CGGAGGAACG ACGACATTAT AAACTTCTTC 
AATGACTTCA GTGACCACCT GGCTGAGGAG GCCCTATGGG AACTCTCTCT GAAAATTAAA 
CCCAGGAACA TAACAAGGAG AAAAACAGAC CGGGAAGAGA AGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RAPGEF1
Entrez Gene ID	103239727
Full Name	Rap guanine nucleotide exchange factor 1
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RAPGEF1 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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