PIEZO1 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIEZO1
Entrez Gene ID	103233455
Full Name	piezo type mechanosensitive ion channel component 1
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007994341.1	XP_007992532.1	piezo-type mechanosensitive ion channel component 1 isoform X1
XM_007994342.1	XP_007992533.1	piezo-type mechanosensitive ion channel component 1 isoform X2
XM_007994343.1	XP_007992534.1	piezo-type mechanosensitive ion channel component 1 isoform X3
XM_007994344.1	XP_007992535.1	piezo-type mechanosensitive ion channel component 1 isoform X4

Pathways from BioSystems
Gene Ontology

The following PIEZO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIEZO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCh85932	XM_007994341.1 Latest version!	Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh85933	XM_007994342.1 Latest version!	Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh85934	XM_007994343.1 Latest version!	Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh85935	XM_007994344.1 Latest version!	Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIEZO1 ( XM_007994341.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007992532.1 Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCh85932
Clone ID Related Accession (Same CDS sequence)	XM_007994341.1
Accession Version	XM_007994341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	piezo-type mechanosensitive ion channel component 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735242.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGC ACGTGCTCGG CGCGGTCCTG TACTGGCTGC TGCTGCCCTG CGCGCTGCTG 
GCGGAAGCAA GTTTGCCCTT CCTTGTGGTA GAGAATCCTA GCTCACGGAG CAGTTTAGAA 
GCCAGCACCG CATCCCACAC ACACATCTGG CACGGACGGA CCTTTGCTGG GTGTCTGTCC 
TGTGTTCTTC TGAAAGCCTG CCTGCTCCGT TTCAGCGGGC TCTCGCTGGT CTACCTGCTC 
TTCCTGCTGC TGCTGCCCTG GTTCCCTGGC CCCACCCGGC ATAGCCTCCA AGGTCACACA 
GGCCGCCTCC TGCGGGCGCT GCTGGGCCTC AGCCTGCTCT TCCTGGTGGC CCATCTCGCT 
CTCCAGATCT GCCTGCACAC CGTGCCCCAC CTGGACCAGC TCCTGGGACC CAGCTGCAGC 
CGCTGGGAGA CCCTCTCGCG ACACATAGGG GTCACAAGGC TGGACCTGAA GGACATCCCC 
AACGCCATCC GGCTGGTGGC CCCTGACCTG GGCATCTTGG TGGTCTCCTC TGTCTGCCTC 
GGCCTCTGTG GGCGCCTCGC AAGGAACACC CAGCAGAGCC CACATATGCA GGAGCTGGAT 
GATGACGAGA GGGATGTGGA TGCCAGTCCC CCGGCAGGGC TGCAGGAAGC CGCAACGCTG 
GCCCCTACAC GGAGGTCACG GCTGGCCGCC CGTTTCCGCG TCACTGCCCA CTGGCTGCTG 
GTGGCGGCTG GGCGGGTCCT GGCCGTAACA CTACTCGCAC TGGCAGGCAT CGCCCACCCC 
TCGGCCCTCT CCAGCGTCTA CCTGCTGGTC TTCCTGGCCA TCTGCACCTG GTGGGCCTGC 
CACTTCCCCA TCAGCACGCT GGGCTTCAAC AGACTCTGCG TCGCAGTGGG CTGCTTCGGC 
GCCGGCCATG TCATCTGCCT CTACTGCTAC CAGACGCCCT TGGCCCAGGC TCTGCTCCCG 
CCTGCCGGCA TCTGGGCTAG GGTGCTGGGT CTCAAGGACT TTGTGGGTCC TGCGAACTGT 
TCCAGCCCCC ATGTGCTGGT CCTCAACACC AGCCTGGACT GGCCCATGTA TGCCAGCCCC 
GGCGTCCTGC TGCTGCTGTG CTACGCCACG GCCTCTCTGC GCAAGCTCCG TGTGCACCGC 
CCCTCCGGCC AGAGGAAGGA GGCGGCAAAG GGGGACGAGA CTCGGGAGCT GGAGCTGGCA 
GAGCTGGACC AGTGGCCCCA GGATGGGGAG GCTGCCCAGC ACGTGGTGCC CACAGCACCC 
GACATGGAGG CTGATAACTG CATCGTGCAC GAGCTGACAG GCCAGAGCCC CCTCCGGCAG 
CGGCCTGTGC GGCCCAAGCG GGCTGAGCCC AGGGAGGCAT CCCCACTCCA CAGCCTGGGC 
CACCTCATCA TGGACCAGAG CTATGTGTGC GCACTCATCG CCATGATGGT GTGGAGCATC 
ACCTACCACA GCTGGCTGAC CTTCGTGCTG CTGCTCTGGG CCTGCCTCAT CTGGACGGTG 
CGCAGCCGCC ACCAGCTGGC CATGCTGTGC TCGCCCTGCA TCCTGCTGTA TGGGATGACG 
CTGTGCTGTC TACGCTACGT GTGGGCCATG GACCTGCGCC CTGAGCTGCC CACCACTCTG 
GGCCCCGTCA GCCTGCGCCA GCTGGGGCTG GAGCACACCC GCTACCCCTG CCTGGACCTT 
GGCGCCATGT TGCTCTACAC CCTGACCTTC TGGCTCCTGC TGCGCCAGTT TGTGAAAGAG 
AAGCTGCTGA AGCGGGCAGA GGCTCCAGCT GCGCTAATGG AGGTCACTGT GGCAGACACA 
GAGCCCACAC GGACGCAGAC GCTTCTGCAG AGCCTGGGGG AGCTGGTGAA GGGCATCTAT 
GCCAAGTACT GGATCTATGT GTGTGCCGGC ATGTTCATCG TGGTCAGCTT CGCCGGCCGC 
CTCGTGGTCT ACAAGATTGT CTACATGTTC CTCTTCCTGC TCTGCCTCAC CCTCTTCCAG 
GTCTACTACA GCCTGTGGCG GAAGCTCCTC AAGGCCTTCT GGTGGCTCGT GGTGGCTTAC 
ACCATGCTGG TCCTCATCGC CGTCTACACC TTCCAGTTCC AGGACTTCCC CGCCTACTGG 
CGCAACCTCA CTGGCTTCAC CGATGAGCAG CTGGGGGACC TGGGCCTGGA GCAGTTCAGC 
GTGTCCGAGC TCTTCTCCAG CATCCTGGTG CCCGGCTTCT TCCTGCTGGC CTGTATCCTG 
CAGCTGCACT ACTTCCACCG GCCCTTTATG CAGCTCACCG ACATGGAGCA TGTGTCCCCG 
CCCCGCAGGC GCCTCCCTCG CTGGGCTCAC AGGCAGGATG CAGTGAGCGG GACCCCACTG 
CTGCAGCAGC AGCAGCAGGA GGAGGAGGAG GAGGAGGGCC CCAGGGATGA GGGGCTGGGC 
ATGGCCACTC CCCACCAGGC CACCCAGGTG CCTGAAGGCG CAGCCAAGTG GGGCCTGGTG 
GCCGAGCGGC TGCTGGAGCT GGCAGCTGGC TTTTCGGACG TCCTCTCACG GGTGCAGGTG 
TTCCTGCGGC GGCTGCTGGA GCTGCACGTC TTCAAGCTGG TGGCCCTGTA CACCGTCTGG 
GTGGCCCTGA AGGAGGTGTC AGTGATGAAC CTGCTGCTGG TGGTGCTGTG GGCCTTCGCC 
CTGCCCTACC CACGCTTCCG ACCCATGGCC TCCTGCCTGT CTACTGTGTG GACCTGTGTC 
ATCATCGTGT GTAAGATGCT GTACCAGCTC AAGGTCGTCA ACCCTCAGGA GTATTCCAGT 
AACTGCACCG AGCCCTCCCC CAACAGCACC AACCTGCTGA CCATGGAGAT CAACCAGTCC 
CTGCTGTACC GGGGGCCCGT GGATCCCGCC AACTGGTTTG GGGTGCGGAA AGGGTTCCCC 
AACCTGGGCT ACATCCAGAA CCACCTGCAG GTCCTGCTGC TGCTGGTATT CGAGGCCATC 
GTGTACCGGC GCCAGGAGCA CCACCGCCGG CAGCACCAGC TGGCCCCGCT GCCTGCCCAG 
GCCGTGTTCG CCAGCGGCAC CCGCCAGCAG CTGGACCAGG ATCTGCTCGG CTGCCTCAAG 
TACTTCATCA ACTTCTTCTT CTACAAATTT GGGCTGGAGA TCTGCTTCTT GATGGCCGTG 
AACGTGATTG GGCAGCGCAT GAACTTTATG GTGACCCTGC ACGGCTGCTG GCTGGTGGCC 
ATCCTCACCC GCAGACACCG CCAGGCCATC GCCCGCCTCT GGCCCAACTA CTGCCTCTTC 
CTGGCTCTGT TCCTGCTGTA CCAGTACCTG CTGTGTCTGG GGATGCCCCC GGCCCTGTGC 
ATCGATTACC CGTGGCGCTG GAGCCGGGCT GTCCCCATGA ACTCCGCACT CATCAAGTGG 
CTGTACCTGC CTGATTTCTT CCGGGCTCCC AACTCCACCA ACCTCATCAG TGACTTCCTC 
CTGCTGCTGT GCGCCTCCCA GCAGTGGCAA GTGTTCTCAG CTGAGCGCAC GGAGGAGTGG 
CAGTGCATGG CTGGTGTCAA CACCGACCGC CTGGAGCCGC TGCGGGGGGA GCCCAACCCT 
GTGCCCAACT TTATCCACTG CAGGTCCTAC CTCGACATGC TGAAGGTGGC CGTCTTCCGG 
TACCTGTTCT GGCTGGTGCT GGTGGTGGTG TTTGTCACGG GGGCCACCCG AATCAGCATC 
TTCGGGCTGG GCTACCTGCT GGCCTGCTTC TACCTGCTGC TCTTCGGCAC CGCCCTGCTG 
CAGAGGGACA CGCGGGCCCG CCTCGTGCTG TGGGACTGCC TCATTCTGTA CAACGTCACC 
GTCATCATCT CCAAGAACAT GCTGTCGCTC CTGGCCTGCG TCTTCGTGGA GCAGATGCAG 
ACCAGCTTCT GCTGGGTCAT CCAGCTCTTC AGCCTTGTGT GTACCGTCAA GGGCTACTAT 
GACCCCAAGG AGATGATGGG CAGAGACCAG GACTGCCTGC TGCCTGTGGA GGAGGCCGGC 
ATCATCTGGG ACAGCATCTG CTTCTTCTTC CTGCTGCTGC AGCGCCGTGT CTTCCTCAGC 
TATTACTACC TGCACGTCAG GGCCGACCTC CAGGCCACTG CCCTGCTGGC CTCCAGGGGC 
TTTGCCCTCT ACAACGCTGC CAACCTCAAG AGCATTGACT TCCACCGCAG GACAGAGGAG 
AAGTCCCTGG CCCAGCTGAA AAGACAGATG GAGCGTATCC GTGCCAAGCA GGAGAAGCAC 
AGGCAGGGCC GGGCGGACCG CGGTCACCCC CAGGACACCC TGGGCCCCAA GGACCCAGGC 
CTGGAGCCAG GGCCCGACAG TCCAGGGGGC TCCTCCCCGC CACGGAGGCA GTGGTGGCGG 
CCCTGGCTGG ACCACGCCAC AGTCATCCAC TCCGGGGACT ACTTCCTGTT TGAGTCTGAC 
AGCGAGGAAG AGGAGGAGGC TGCCCCTGAA GACCCTAGGC CATCGGCCCA GAGTGCCTTC 
CAGATGGCAT ACCAGGCATG GGTGACCAAC GCCCAGACGG TGCTGAGGCG GCAGCAGCAG 
GAGCAGGCAA GGCAGAAGCA GGCAGGACAG CTGCCCACAG GAGGTGGCCC CAGCCAGGAG 
GTGGAGCCGG CAGAGGGCCC CGAGGAGGCA GCGGCAGGCC GGAGCCACAT GGTGCAGAGG 
GTGCTGAGCA CGGCGCAGTT CCTCTGGGTG CTGGGGCAGG CGCTGGTGGA CGGGCTGACG 
CGCTGGCTGC AGGAGTTCAC CCGGCACCAC AGCACCATGA GTGACGTGCT GCGGGCAGAG 
CGCTACGTCC TCACGCAGGA GCTCCTGCAG GGTGGTGAAG TGCACCGGGG CATATTGGAT 
CAGCTGTACA CAAGCGAGGC TGAGGCCACA CTGCCAGGCC CCACCGAGGC CCCCGACGCA 
CCAAGCACAG TGTCCAGTGG GCTGGGCGCG GAGGAGCCAC TCAGCAGCAT GACAGACGAC 
ACGGGCAGCC CCCTGAGCAC TGGCTACCAC ACGCGCAGTG GCAGCGAGGA GGTGGTCGCT 
GACCCCGGGG AGCACGAGGC TGGCGCCTCT CTGCACCAGG GACGCATGCG GACGGCCAGC 
GAGCTGCTGC TGGACAGGCG CTTGCGCATC CCAGAGCTGG AGGAGGCAGA GCTGTTTGCG 
GAGGGGCAGG GCCGGGCGCT GCGGCTGCTG CGGGCCGTGT ACCAGTGCGT GGCTGCCCAC 
TCAGAGCTGC TCTGCTACTT CATCATCATC CTCAACCACA TGGTCACGGC CTCTGCGGGC 
TCGCTGGTGC TGCCCGTGCT CGTCTTCCTG TGGGCCATGC TGTCCATCCC GCGGCCCAGC 
AAGCGCTTCT GGATGACGGC CATCGTCTTC ACCGAGGTCT CAGTGGTCGT CAAGTACCTG 
TTCCAGTTTG GATTCTTCCC CTGGAACAGC CACGTGGTGC TGCGGCGCTA CGAGAACAAG 
CCCTACTTCC CGCCCCGCAT CCTGGGCCTG GAGAAGACTG ACGGCTACAT CAAGTACGAC 
CTGGTGCAGC TCATGGCCCT CTTCTTCCAC CGCTCCCAGT TGCTGTGCTA TGGCCTCTGG 
GACCACGAGG AGGACTCACC ATCCAAGGAG CATGACAAGA GCGGCAGGAA GGAGCAGGGG 
GCCGAGGAGG GGCCAGGGGC CCTGCCGGAA CCCCAGGCCA AGGCAGGCAC AGGGCTCGAG 
GAGGAGCCAG GAGTGCCCGA GGCCACCACC GAGGACCATG TTCAGGTGGA AGCAAGGGTT 
GTACCGACGG ATGGGACCCC AGAACCCCAA GTGGAGCTCA GGCCCCGTGA CACGAGGCGC 
ATCAGTCTAC GTTTGAGGAG GAGGAGGAAG GAGGGCCCAG CACAGAGAGG ACTGGCTGCC 
ATCGAAGCTG AGGACAGGGA GGAAGAGAAA GAGGCCCCCA CGGGGAGAGA GAAGAGGCCA 
AGCCGCGCTG GAAGAAGAGT GAGGGCGGCT GGGCGGCGGC TGCAGGGCTT CTGTCTGTCC 
CTGGCCCAGG GCACATACCG GCCGCTACGG CGCTTCTTCC ATGACATCCT GCACACCAAG 
TACCGCGCAG CCACCGACGT CTATGCCCTC ATGTTCCTGG CCGACGTCGT CGACTTCATC 
ATCATCATTT TTGGCTTCTG GGCCTTTGGG AAACACTCAG CGGCCACGGA TATCACGTCC 
TCCCTATCAG ACGACCAGGT ACCCGAGGCC TTCCTGGTCA TGCTGCTGAT CCAGTTCAGT 
ACCATGGTGG TCGACCGCGC CCTCTACCTG CGCAAGACTG TGCTGGGCAA GCTGGCCTTC 
CAGGTGGCGC TGGTGCTGGC CATCCACCTA TGGATGTTCT TCATCCTGCC TGCCGTCACT 
GAGAGGATGT TCAGCCAGAA CGTGGTGGCC CAGCTGTGGT ACTTTGTGAA ATGCATCTAC 
TTCGCCCTGT CCGCCTACCA GATCCGCTGT GGCTACCCCA CCCGCATCCT TGGCAACTTC 
CTCACCAAGA AGTACAATCA CCTCAACCTC TTCCTTTTCC AGGGGTTCCG GCTGGTGCCG 
TTTCTGGTGG AGCTGCGGGC AGTGATGGAC TGGGTGTGGA CGGACACCAC GCTGTCCCTG 
TCCAGCTGGA TGTGCGTGGA GGACATCTAT GCCAACATCT TCATCATCAA ATGCAGCCGA 
GAGACAGAAA AGAAATATCC ACAGCCCAAA GGGCAGAAGA AGAAGAAGAT CGTCAAGTAC 
GGCATGGGTG GCCTCATCAT CCTCTTCCTC ATCGCCATCA TCTGGTTCCC ACTGCTTTTC 
ATGTCACTGG TGCGTTCCGT GGTCGGGGTC GTCAACCAGC CCGTCGATGT CACCGTCACC 
CTCAAGCTGG GCGGCTATGA GCCACTGTTC ACCATGAGCG CCCAGCAGCC GTCCATCATC 
CCCTTCACGG CCCAGGCCTA CGAGGAGCTG TCCCGGCAGT TTGACCCTCA CCCACTGGCC 
ATGCAGTTCA TCAGCCAGTA CAGCCCTGAG GACATTGTCA CGGCGCAGAT TGAGGGCAGC 
TCCGGGGCGC TGTGGCGCAT CAGCCCACCC AGCCGCGCCC AGATGAAGCG AGAGCTCTAC 
AACGGCACGG CTGACATCAC CCTGCGTTTC ACCTGGAACT TCCAGAGGGA CCTGGCCAAG 
GGAGGCACCG TGGAGTACGC CAACGAGAAG CACATGCTGG CCCTGGCCCC CAACAGCACC 
GCACGGCGGC AGCTGGCCAG CCTGCTCGAG GGCACCTCGG ACCAGTCTGT GGTCATCCCA 
AATCTCTTCC CCAAGTATAT CCGTGCCCCC AACGGGCCCG AAGCCAACCC TGTGAAGCAG 
CTGCAGCCCA ACGAGGAGGC CGACTACCTC GGCGTGCGCA TCCAGCTGCG GAGGGAGCAG 
GGTGCGGGGG CCACCGGCTT CCTTGAATGG TGGGTCATCG AGCTGCAGGA GTGCCGGACC 
GACTGCAACC TACTGCCCAT GGTCATCTTC AGCGACAAAG TCAGCCCACC GAGCCTCGGC 
TTCCTGGCTG GCTACGGGAT CATGGGGCTG TATGTCTCCA TCGTGCTGGT CATCGGCAAG 
TTCGTGCGTG GATTCTTCAG CGAGATCTCG CACTCCATTA TGTTCGAGGA GCTGCCGTGC 
GTGGACCGCA TCCTCAAGCT CTGCCAGGAC ATCTTCCTGG TGCGGGAGAC TCGTGAGCTG 
GAGCTGGAGG AGGAGTTGTA CGCCAAGCTC ATCTTCCTCT ACCGCTCACC AGAGACCATG 
ATCAAGTGGA CTCGTGAGAA GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007992532.1
CDS	270..7976
Translation	MEPHVLGAVLYWLLLPCALLAEASLPFLVVENPSSRSSLEASTASHTHIWHGRTFAGCLSCVLLKACLLRFSGLSLVYLLFLLLLPWFPGPTRHSLQGHTGRLLRALLGLSLLFLVAHLALQICLHTVPHLDQLLGPSCSRWETLSRHIGVTRLDLKDIPNAIRLVAPDLGILVVSSVCLGLCGRLARNTQQSPHMQELDDDERDVDASPPAGLQEAATLAPTRRSRLAARFRVTAHWLLVAAGRVLAVTLLALAGIAHPSALSSVYLLVFLAICTWWACHFPISTLGFNRLCVAVGCFGAGHVICLYCYQTPLAQALLPPAGIWARVLGLKDFVGPANCSSPHVLVLNTSLDWPMYASPGVLLLLCYATASLRKLRVHRPSGQRKEAAKGDETRELELAELDQWPQDGEAAQHVVPTAPDMEADNCIVHELTGQSPLRQRPVRPKRAEPREASPLHSLGHLIMDQSYVCALIAMMVWSITYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGMTLCCLRYVWAMDLRPELPTTLGPVSLRQLGLEHTRYPCLDLGAMLLYTLTFWLLLRQFVKEKLLKRAEAPAALMEVTVADTEPTRTQTLLQSLGELVKGIYAKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYSLWRKLLKAFWWLVVAYTMLVLIAVYTFQFQDFPAYWRNLTGFTDEQLGDLGLEQFSVSELFSSILVPGFFLLACILQLHYFHRPFMQLTDMEHVSPPRRRLPRWAHRQDAVSGTPLLQQQQQEEEEEEGPRDEGLGMATPHQATQVPEGAAKWGLVAERLLELAAGFSDVLSRVQVFLRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCVIIVCKMLYQLKVVNPQEYSSNCTEPSPNSTNLLTMEINQSLLYRGPVDPANWFGVRKGFPNLGYIQNHLQVLLLLVFEAIVYRRQEHHRRQHQLAPLPAQAVFASGTRQQLDQDLLGCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVTLHGCWLVAILTRRHRQAIARLWPNYCLFLALFLLYQYLLCLGMPPALCIDYPWRWSRAVPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQCMAGVNTDRLEPLRGEPNPVPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTALLQRDTRARLVLWDCLILYNVTVIISKNMLSLLACVFVEQMQTSFCWVIQLFSLVCTVKGYYDPKEMMGRDQDCLLPVEEAGIIWDSICFFFLLLQRRVFLSYYYLHVRADLQATALLASRGFALYNAANLKSIDFHRRTEEKSLAQLKRQMERIRAKQEKHRQGRADRGHPQDTLGPKDPGLEPGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEAAPEDPRPSAQSAFQMAYQAWVTNAQTVLRRQQQEQARQKQAGQLPTGGGPSQEVEPAEGPEEAAAGRSHMVQRVLSTAQFLWVLGQALVDGLTRWLQEFTRHHSTMSDVLRAERYVLTQELLQGGEVHRGILDQLYTSEAEATLPGPTEAPDAPSTVSSGLGAEEPLSSMTDDTGSPLSTGYHTRSGSEEVVADPGEHEAGASLHQGRMRTASELLLDRRLRIPELEEAELFAEGQGRALRLLRAVYQCVAAHSELLCYFIIILNHMVTASAGSLVLPVLVFLWAMLSIPRPSKRFWMTAIVFTEVSVVVKYLFQFGFFPWNSHVVLRRYENKPYFPPRILGLEKTDGYIKYDLVQLMALFFHRSQLLCYGLWDHEEDSPSKEHDKSGRKEQGAEEGPGALPEPQAKAGTGLEEEPGVPEATTEDHVQVEARVVPTDGTPEPQVELRPRDTRRISLRLRRRRKEGPAQRGLAAIEAEDREEEKEAPTGREKRPSRAGRRVRAAGRRLQGFCLSLAQGTYRPLRRFFHDILHTKYRAATDVYALMFLADVVDFIIIIFGFWAFGKHSAATDITSSLSDDQVPEAFLVMLLIQFSTMVVDRALYLRKTVLGKLAFQVALVLAIHLWMFFILPAVTERMFSQNVVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSSWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLVRSVVGVVNQPVDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPHPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Target ORF information:

RefSeq Version	XM_007994341.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007994341.1

ORF Insert Sequence:

ATGGAACCGC ACGTGCTCGG CGCGGTCCTG TACTGGCTGC TGCTGCCCTG CGCGCTGCTG 
GCGGAAGCAA GTTTGCCCTT CCTTGTGGTA GAGAATCCTA GCTCACGGAG CAGTTTAGAA 
GCCAGCACCG CATCCCACAC ACACATCTGG CACGGACGGA CCTTTGCTGG GTGTCTGTCC 
TGTGTTCTTC TGAAAGCCTG CCTGCTCCGT TTCAGCGGGC TCTCGCTGGT CTACCTGCTC 
TTCCTGCTGC TGCTGCCCTG GTTCCCTGGC CCCACCCGGC ATAGCCTCCA AGGTCACACA 
GGCCGCCTCC TGCGGGCGCT GCTGGGCCTC AGCCTGCTCT TCCTGGTGGC CCATCTCGCT 
CTCCAGATCT GCCTGCACAC CGTGCCCCAC CTGGACCAGC TCCTGGGACC CAGCTGCAGC 
CGCTGGGAGA CCCTCTCGCG ACACATAGGG GTCACAAGGC TGGACCTGAA GGACATCCCC 
AACGCCATCC GGCTGGTGGC CCCTGACCTG GGCATCTTGG TGGTCTCCTC TGTCTGCCTC 
GGCCTCTGTG GGCGCCTCGC AAGGAACACC CAGCAGAGCC CACATATGCA GGAGCTGGAT 
GATGACGAGA GGGATGTGGA TGCCAGTCCC CCGGCAGGGC TGCAGGAAGC CGCAACGCTG 
GCCCCTACAC GGAGGTCACG GCTGGCCGCC CGTTTCCGCG TCACTGCCCA CTGGCTGCTG 
GTGGCGGCTG GGCGGGTCCT GGCCGTAACA CTACTCGCAC TGGCAGGCAT CGCCCACCCC 
TCGGCCCTCT CCAGCGTCTA CCTGCTGGTC TTCCTGGCCA TCTGCACCTG GTGGGCCTGC 
CACTTCCCCA TCAGCACGCT GGGCTTCAAC AGACTCTGCG TCGCAGTGGG CTGCTTCGGC 
GCCGGCCATG TCATCTGCCT CTACTGCTAC CAGACGCCCT TGGCCCAGGC TCTGCTCCCG 
CCTGCCGGCA TCTGGGCTAG GGTGCTGGGT CTCAAGGACT TTGTGGGTCC TGCGAACTGT 
TCCAGCCCCC ATGTGCTGGT CCTCAACACC AGCCTGGACT GGCCCATGTA TGCCAGCCCC 
GGCGTCCTGC TGCTGCTGTG CTACGCCACG GCCTCTCTGC GCAAGCTCCG TGTGCACCGC 
CCCTCCGGCC AGAGGAAGGA GGCGGCAAAG GGGGACGAGA CTCGGGAGCT GGAGCTGGCA 
GAGCTGGACC AGTGGCCCCA GGATGGGGAG GCTGCCCAGC ACGTGGTGCC CACAGCACCC 
GACATGGAGG CTGATAACTG CATCGTGCAC GAGCTGACAG GCCAGAGCCC CCTCCGGCAG 
CGGCCTGTGC GGCCCAAGCG GGCTGAGCCC AGGGAGGCAT CCCCACTCCA CAGCCTGGGC 
CACCTCATCA TGGACCAGAG CTATGTGTGC GCACTCATCG CCATGATGGT GTGGAGCATC 
ACCTACCACA GCTGGCTGAC CTTCGTGCTG CTGCTCTGGG CCTGCCTCAT CTGGACGGTG 
CGCAGCCGCC ACCAGCTGGC CATGCTGTGC TCGCCCTGCA TCCTGCTGTA TGGGATGACG 
CTGTGCTGTC TACGCTACGT GTGGGCCATG GACCTGCGCC CTGAGCTGCC CACCACTCTG 
GGCCCCGTCA GCCTGCGCCA GCTGGGGCTG GAGCACACCC GCTACCCCTG CCTGGACCTT 
GGCGCCATGT TGCTCTACAC CCTGACCTTC TGGCTCCTGC TGCGCCAGTT TGTGAAAGAG 
AAGCTGCTGA AGCGGGCAGA GGCTCCAGCT GCGCTAATGG AGGTCACTGT GGCAGACACA 
GAGCCCACAC GGACGCAGAC GCTTCTGCAG AGCCTGGGGG AGCTGGTGAA GGGCATCTAT 
GCCAAGTACT GGATCTATGT GTGTGCCGGC ATGTTCATCG TGGTCAGCTT CGCCGGCCGC 
CTCGTGGTCT ACAAGATTGT CTACATGTTC CTCTTCCTGC TCTGCCTCAC CCTCTTCCAG 
GTCTACTACA GCCTGTGGCG GAAGCTCCTC AAGGCCTTCT GGTGGCTCGT GGTGGCTTAC 
ACCATGCTGG TCCTCATCGC CGTCTACACC TTCCAGTTCC AGGACTTCCC CGCCTACTGG 
CGCAACCTCA CTGGCTTCAC CGATGAGCAG CTGGGGGACC TGGGCCTGGA GCAGTTCAGC 
GTGTCCGAGC TCTTCTCCAG CATCCTGGTG CCCGGCTTCT TCCTGCTGGC CTGTATCCTG 
CAGCTGCACT ACTTCCACCG GCCCTTTATG CAGCTCACCG ACATGGAGCA TGTGTCCCCG 
CCCCGCAGGC GCCTCCCTCG CTGGGCTCAC AGGCAGGATG CAGTGAGCGG GACCCCACTG 
CTGCAGCAGC AGCAGCAGGA GGAGGAGGAG GAGGAGGGCC CCAGGGATGA GGGGCTGGGC 
ATGGCCACTC CCCACCAGGC CACCCAGGTG CCTGAAGGCG CAGCCAAGTG GGGCCTGGTG 
GCCGAGCGGC TGCTGGAGCT GGCAGCTGGC TTTTCGGACG TCCTCTCACG GGTGCAGGTG 
TTCCTGCGGC GGCTGCTGGA GCTGCACGTC TTCAAGCTGG TGGCCCTGTA CACCGTCTGG 
GTGGCCCTGA AGGAGGTGTC AGTGATGAAC CTGCTGCTGG TGGTGCTGTG GGCCTTCGCC 
CTGCCCTACC CACGCTTCCG ACCCATGGCC TCCTGCCTGT CTACTGTGTG GACCTGTGTC 
ATCATCGTGT GTAAGATGCT GTACCAGCTC AAGGTCGTCA ACCCTCAGGA GTATTCCAGT 
AACTGCACCG AGCCCTCCCC CAACAGCACC AACCTGCTGA CCATGGAGAT CAACCAGTCC 
CTGCTGTACC GGGGGCCCGT GGATCCCGCC AACTGGTTTG GGGTGCGGAA AGGGTTCCCC 
AACCTGGGCT ACATCCAGAA CCACCTGCAG GTCCTGCTGC TGCTGGTATT CGAGGCCATC 
GTGTACCGGC GCCAGGAGCA CCACCGCCGG CAGCACCAGC TGGCCCCGCT GCCTGCCCAG 
GCCGTGTTCG CCAGCGGCAC CCGCCAGCAG CTGGACCAGG ATCTGCTCGG CTGCCTCAAG 
TACTTCATCA ACTTCTTCTT CTACAAATTT GGGCTGGAGA TCTGCTTCTT GATGGCCGTG 
AACGTGATTG GGCAGCGCAT GAACTTTATG GTGACCCTGC ACGGCTGCTG GCTGGTGGCC 
ATCCTCACCC GCAGACACCG CCAGGCCATC GCCCGCCTCT GGCCCAACTA CTGCCTCTTC 
CTGGCTCTGT TCCTGCTGTA CCAGTACCTG CTGTGTCTGG GGATGCCCCC GGCCCTGTGC 
ATCGATTACC CGTGGCGCTG GAGCCGGGCT GTCCCCATGA ACTCCGCACT CATCAAGTGG 
CTGTACCTGC CTGATTTCTT CCGGGCTCCC AACTCCACCA ACCTCATCAG TGACTTCCTC 
CTGCTGCTGT GCGCCTCCCA GCAGTGGCAA GTGTTCTCAG CTGAGCGCAC GGAGGAGTGG 
CAGTGCATGG CTGGTGTCAA CACCGACCGC CTGGAGCCGC TGCGGGGGGA GCCCAACCCT 
GTGCCCAACT TTATCCACTG CAGGTCCTAC CTCGACATGC TGAAGGTGGC CGTCTTCCGG 
TACCTGTTCT GGCTGGTGCT GGTGGTGGTG TTTGTCACGG GGGCCACCCG AATCAGCATC 
TTCGGGCTGG GCTACCTGCT GGCCTGCTTC TACCTGCTGC TCTTCGGCAC CGCCCTGCTG 
CAGAGGGACA CGCGGGCCCG CCTCGTGCTG TGGGACTGCC TCATTCTGTA CAACGTCACC 
GTCATCATCT CCAAGAACAT GCTGTCGCTC CTGGCCTGCG TCTTCGTGGA GCAGATGCAG 
ACCAGCTTCT GCTGGGTCAT CCAGCTCTTC AGCCTTGTGT GTACCGTCAA GGGCTACTAT 
GACCCCAAGG AGATGATGGG CAGAGACCAG GACTGCCTGC TGCCTGTGGA GGAGGCCGGC 
ATCATCTGGG ACAGCATCTG CTTCTTCTTC CTGCTGCTGC AGCGCCGTGT CTTCCTCAGC 
TATTACTACC TGCACGTCAG GGCCGACCTC CAGGCCACTG CCCTGCTGGC CTCCAGGGGC 
TTTGCCCTCT ACAACGCTGC CAACCTCAAG AGCATTGACT TCCACCGCAG GACAGAGGAG 
AAGTCCCTGG CCCAGCTGAA AAGACAGATG GAGCGTATCC GTGCCAAGCA GGAGAAGCAC 
AGGCAGGGCC GGGCGGACCG CGGTCACCCC CAGGACACCC TGGGCCCCAA GGACCCAGGC 
CTGGAGCCAG GGCCCGACAG TCCAGGGGGC TCCTCCCCGC CACGGAGGCA GTGGTGGCGG 
CCCTGGCTGG ACCACGCCAC AGTCATCCAC TCCGGGGACT ACTTCCTGTT TGAGTCTGAC 
AGCGAGGAAG AGGAGGAGGC TGCCCCTGAA GACCCTAGGC CATCGGCCCA GAGTGCCTTC 
CAGATGGCAT ACCAGGCATG GGTGACCAAC GCCCAGACGG TGCTGAGGCG GCAGCAGCAG 
GAGCAGGCAA GGCAGAAGCA GGCAGGACAG CTGCCCACAG GAGGTGGCCC CAGCCAGGAG 
GTGGAGCCGG CAGAGGGCCC CGAGGAGGCA GCGGCAGGCC GGAGCCACAT GGTGCAGAGG 
GTGCTGAGCA CGGCGCAGTT CCTCTGGGTG CTGGGGCAGG CGCTGGTGGA CGGGCTGACG 
CGCTGGCTGC AGGAGTTCAC CCGGCACCAC AGCACCATGA GTGACGTGCT GCGGGCAGAG 
CGCTACGTCC TCACGCAGGA GCTCCTGCAG GGTGGTGAAG TGCACCGGGG CATATTGGAT 
CAGCTGTACA CAAGCGAGGC TGAGGCCACA CTGCCAGGCC CCACCGAGGC CCCCGACGCA 
CCAAGCACAG TGTCCAGTGG GCTGGGCGCG GAGGAGCCAC TCAGCAGCAT GACAGACGAC 
ACGGGCAGCC CCCTGAGCAC TGGCTACCAC ACGCGCAGTG GCAGCGAGGA GGTGGTCGCT 
GACCCCGGGG AGCACGAGGC TGGCGCCTCT CTGCACCAGG GACGCATGCG GACGGCCAGC 
GAGCTGCTGC TGGACAGGCG CTTGCGCATC CCAGAGCTGG AGGAGGCAGA GCTGTTTGCG 
GAGGGGCAGG GCCGGGCGCT GCGGCTGCTG CGGGCCGTGT ACCAGTGCGT GGCTGCCCAC 
TCAGAGCTGC TCTGCTACTT CATCATCATC CTCAACCACA TGGTCACGGC CTCTGCGGGC 
TCGCTGGTGC TGCCCGTGCT CGTCTTCCTG TGGGCCATGC TGTCCATCCC GCGGCCCAGC 
AAGCGCTTCT GGATGACGGC CATCGTCTTC ACCGAGGTCT CAGTGGTCGT CAAGTACCTG 
TTCCAGTTTG GATTCTTCCC CTGGAACAGC CACGTGGTGC TGCGGCGCTA CGAGAACAAG 
CCCTACTTCC CGCCCCGCAT CCTGGGCCTG GAGAAGACTG ACGGCTACAT CAAGTACGAC 
CTGGTGCAGC TCATGGCCCT CTTCTTCCAC CGCTCCCAGT TGCTGTGCTA TGGCCTCTGG 
GACCACGAGG AGGACTCACC ATCCAAGGAG CATGACAAGA GCGGCAGGAA GGAGCAGGGG 
GCCGAGGAGG GGCCAGGGGC CCTGCCGGAA CCCCAGGCCA AGGCAGGCAC AGGGCTCGAG 
GAGGAGCCAG GAGTGCCCGA GGCCACCACC GAGGACCATG TTCAGGTGGA AGCAAGGGTT 
GTACCGACGG ATGGGACCCC AGAACCCCAA GTGGAGCTCA GGCCCCGTGA CACGAGGCGC 
ATCAGTCTAC GTTTGAGGAG GAGGAGGAAG GAGGGCCCAG CACAGAGAGG ACTGGCTGCC 
ATCGAAGCTG AGGACAGGGA GGAAGAGAAA GAGGCCCCCA CGGGGAGAGA GAAGAGGCCA 
AGCCGCGCTG GAAGAAGAGT GAGGGCGGCT GGGCGGCGGC TGCAGGGCTT CTGTCTGTCC 
CTGGCCCAGG GCACATACCG GCCGCTACGG CGCTTCTTCC ATGACATCCT GCACACCAAG 
TACCGCGCAG CCACCGACGT CTATGCCCTC ATGTTCCTGG CCGACGTCGT CGACTTCATC 
ATCATCATTT TTGGCTTCTG GGCCTTTGGG AAACACTCAG CGGCCACGGA TATCACGTCC 
TCCCTATCAG ACGACCAGGT ACCCGAGGCC TTCCTGGTCA TGCTGCTGAT CCAGTTCAGT 
ACCATGGTGG TCGACCGCGC CCTCTACCTG CGCAAGACTG TGCTGGGCAA GCTGGCCTTC 
CAGGTGGCGC TGGTGCTGGC CATCCACCTA TGGATGTTCT TCATCCTGCC TGCCGTCACT 
GAGAGGATGT TCAGCCAGAA CGTGGTGGCC CAGCTGTGGT ACTTTGTGAA ATGCATCTAC 
TTCGCCCTGT CCGCCTACCA GATCCGCTGT GGCTACCCCA CCCGCATCCT TGGCAACTTC 
CTCACCAAGA AGTACAATCA CCTCAACCTC TTCCTTTTCC AGGGGTTCCG GCTGGTGCCG 
TTTCTGGTGG AGCTGCGGGC AGTGATGGAC TGGGTGTGGA CGGACACCAC GCTGTCCCTG 
TCCAGCTGGA TGTGCGTGGA GGACATCTAT GCCAACATCT TCATCATCAA ATGCAGCCGA 
GAGACAGAAA AGAAATATCC ACAGCCCAAA GGGCAGAAGA AGAAGAAGAT CGTCAAGTAC 
GGCATGGGTG GCCTCATCAT CCTCTTCCTC ATCGCCATCA TCTGGTTCCC ACTGCTTTTC 
ATGTCACTGG TGCGTTCCGT GGTCGGGGTC GTCAACCAGC CCGTCGATGT CACCGTCACC 
CTCAAGCTGG GCGGCTATGA GCCACTGTTC ACCATGAGCG CCCAGCAGCC GTCCATCATC 
CCCTTCACGG CCCAGGCCTA CGAGGAGCTG TCCCGGCAGT TTGACCCTCA CCCACTGGCC 
ATGCAGTTCA TCAGCCAGTA CAGCCCTGAG GACATTGTCA CGGCGCAGAT TGAGGGCAGC 
TCCGGGGCGC TGTGGCGCAT CAGCCCACCC AGCCGCGCCC AGATGAAGCG AGAGCTCTAC 
AACGGCACGG CTGACATCAC CCTGCGTTTC ACCTGGAACT TCCAGAGGGA CCTGGCCAAG 
GGAGGCACCG TGGAGTACGC CAACGAGAAG CACATGCTGG CCCTGGCCCC CAACAGCACC 
GCACGGCGGC AGCTGGCCAG CCTGCTCGAG GGCACCTCGG ACCAGTCTGT GGTCATCCCA 
AATCTCTTCC CCAAGTATAT CCGTGCCCCC AACGGGCCCG AAGCCAACCC TGTGAAGCAG 
CTGCAGCCCA ACGAGGAGGC CGACTACCTC GGCGTGCGCA TCCAGCTGCG GAGGGAGCAG 
GGTGCGGGGG CCACCGGCTT CCTTGAATGG TGGGTCATCG AGCTGCAGGA GTGCCGGACC 
GACTGCAACC TACTGCCCAT GGTCATCTTC AGCGACAAAG TCAGCCCACC GAGCCTCGGC 
TTCCTGGCTG GCTACGGGAT CATGGGGCTG TATGTCTCCA TCGTGCTGGT CATCGGCAAG 
TTCGTGCGTG GATTCTTCAG CGAGATCTCG CACTCCATTA TGTTCGAGGA GCTGCCGTGC 
GTGGACCGCA TCCTCAAGCT CTGCCAGGAC ATCTTCCTGG TGCGGGAGAC TCGTGAGCTG 
GAGCTGGAGG AGGAGTTGTA CGCCAAGCTC ATCTTCCTCT ACCGCTCACC AGAGACCATG 
ATCAAGTGGA CTCGTGAGAA GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO1 ( XM_007994342.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007992533.1 Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA.

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CloneID	OCh85933
Clone ID Related Accession (Same CDS sequence)	XM_007994342.1
Accession Version	XM_007994342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	piezo-type mechanosensitive ion channel component 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735242.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGC ACGTGCTCGG CGCGGTCCTG TACTGGCTGC TGCTGCCCTG CGCGCTGCTG 
GCGGAAGCAA GTTTGCCCTT CCTTGTGGTA GAGAATCCTA GCTCACGGAG CAGTTTAGAA 
GCCAGCACCG CATCCCACAC ACACATCTGG CACGGACGGA CCTTTGCTGG GTGTCTGTCC 
TGTGTTCTTC TGAAAGCCTG CCTGCTCCGT TTCAGCGGGC TCTCGCTGGT CTACCTGCTC 
TTCCTGCTGC TGCTGCCCTG GTTCCCTGGC CCCACCCGGC ATAGCCTCCA AGGTCACACA 
GGCCGCCTCC TGCGGGCGCT GCTGGGCCTC AGCCTGCTCT TCCTGGTGGC CCATCTCGCT 
CTCCAGATCT GCCTGCACAC CGTGCCCCAC CTGGACCAGC TCCTGGGACC CAGCTGCAGC 
CGCTGGGAGA CCCTCTCGCG ACACATAGGG GTCACAAGGC TGGACCTGAA GGACATCCCC 
AACGCCATCC GGCTGGTGGC CCCTGACCTG GGCATCTTGG TGGTCTCCTC TGTCTGCCTC 
GGCCTCTGTG GGCGCCTCGC AAGGAACACC CAGCAGAGCC CACATATGCA GGAGCTGGAT 
GATGACGAGA GGGATGTGGA TGCCAGTCCC CCGGCAGGGC TGCAGGAAGC CGCAACGCTG 
GCCCCTACAC GGAGGTCACG GCTGGCCGCC CGTTTCCGCG TCACTGCCCA CTGGCTGCTG 
GTGGCGGCTG GGCGGGTCCT GGCCGTAACA CTACTCGCAC TGGCAGGCAT CGCCCACCCC 
TCGGCCCTCT CCAGCGTCTA CCTGCTGGTC TTCCTGGCCA TCTGCACCTG GTGGGCCTGC 
CACTTCCCCA TCAGCACGCT GGGCTTCAAC AGACTCTGCG TCGCAGTGGG CTGCTTCGGC 
GCCGGCCATG TCATCTGCCT CTACTGCTAC CAGACGCCCT TGGCCCAGGC TCTGCTCCCG 
CCTGCCGGCA TCTGGGCTAG GGTGCTGGGT CTCAAGGACT TTGTGGGTCC TGCGAACTGT 
TCCAGCCCCC ATGTGCTGGT CCTCAACACC AGCCTGGACT GGCCCATGTA TGCCAGCCCC 
GGCGTCCTGC TGCTGCTGTG CTACGCCACG GCCTCTCTGC GCAAGCTCCG TGTGCACCGC 
CCCTCCGGCC AGAGGAAGGA GGCGGCAAAG GGGGACGAGA CTCGGGAGCT GGAGCTGGCA 
GAGCTGGACC AGTGGCCCCA GGATGGGGAG GCTGCCCAGC ACGTGGTGCC CACAGCACCC 
GACATGGAGG CTGATAACTG CATCGTGCAC GAGCTGACAG GCCAGAGCCC CCTCCGGCAG 
CGGCCTGTGC GGCCCAAGCG GGCTGAGCCC AGGGAGGCAT CCCCACTCCA CAGCCTGGGC 
CACCTCATCA TGGACCAGAG CTATGTGTGC GCACTCATCG CCATGATGGT GTGGAGCATC 
ACCTACCACA GCTGGCTGAC CTTCGTGCTG CTGCTCTGGG CCTGCCTCAT CTGGACGGTG 
CGCAGCCGCC ACCAGCTGGC CATGCTGTGC TCGCCCTGCA TCCTGCTGTA TGGGATGACG 
CTGTGCTGTC TACGCTACGT GTGGGCCATG GACCTGCGCC CTGAGCTGCC CACCACTCTG 
GGCCCCGTCA GCCTGCGCCA GCTGGGGCTG GAGCACACCC GCTACCCCTG CCTGGACCTT 
GGCGCCATGT TGCTCTACAC CCTGACCTTC TGGCTCCTGC TGCGCCAGTT TGTGAAAGAG 
AAGCTGCTGA AGCGGGCAGA GGCTCCAGCT GCGCTAATGG AGGTCACTGT GGCAGACACA 
GAGCCCACAC GGACGCAGAC GCTTCTGCAG AGCCTGGGGG AGCTGGTGAA GGGCATCTAT 
GCCAAGTACT GGATCTATGT GTGTGCCGGC ATGTTCATCG TGGTCAGCTT CGCCGGCCGC 
CTCGTGGTCT ACAAGATTGT CTACATGTTC CTCTTCCTGC TCTGCCTCAC CCTCTTCCAG 
GTCTACTACA GCCTGTGGCG GAAGCTCCTC AAGGCCTTCT GGTGGCTCGT GGTGGCTTAC 
ACCATGCTGG TCCTCATCGC CGTCTACACC TTCCAGTTCC AGGACTTCCC CGCCTACTGG 
CGCAACCTCA CTGGCTTCAC CGATGAGCAG CTGGGGGACC TGGGCCTGGA GCAGTTCAGC 
GTGTCCGAGC TCTTCTCCAG CATCCTGGTG CCCGGCTTCT TCCTGCTGGC CTGTATCCTG 
CAGCTGCACT ACTTCCACCG GCCCTTTATG CAGCTCACCG ACATGGAGCA TGTGTCCCCG 
CCCCGCAGGC GCCTCCCTCG CTGGGCTCAC AGGCAGGATG CAGTGAGCGG GACCCCACTG 
CTGCAGCAGC AGCAGCAGGA GGAGGAGGAG GAGGAGGGCC CCAGGGATGA GGGGCTGGGC 
ATGGCCACTC CCCACCAGGC CACCCAGGTG CCTGAAGGCG CAGCCAAGTG GGGCCTGGTG 
GCCGAGCGGC TGCTGGAGCT GGCAGCTGGC TTTTCGGACG TCCTCTCACG GGTGCAGGTG 
TTCCTGCGGC GGCTGCTGGA GCTGCACGTC TTCAAGCTGG TGGCCCTGTA CACCGTCTGG 
GTGGCCCTGA AGGAGGTGTC AGTGATGAAC CTGCTGCTGG TGGTGCTGTG GGCCTTCGCC 
CTGCCCTACC CACGCTTCCG ACCCATGGCC TCCTGCCTGT CTACTGTGTG GACCTGTGTC 
ATCATCGTGT GTAAGATGCT GTACCAGCTC AAGGTCGTCA ACCCTCAGGA GTATTCCAGT 
AACTGCACCG AGCCCTCCCC CAACAGCACC AACCTGCTGA CCATGGAGAT CAACCAGTCC 
CTGCTGTACC GGGGGCCCGT GGATCCCGCC AACTGGTTTG GGGTGCGGAA AGGGTTCCCC 
AACCTGGGCT ACATCCAGAA CCACCTGCAG GTCCTGCTGC TGCTGGTATT CGAGGCCATC 
GTGTACCGGC GCCAGGAGCA CCACCGCCGG CAGCACCAGC TGGCCCCGCT GCCTGCCCAG 
GCCGTGTTCG CCAGCGGCAC CCGCCAGCAG CTGGACCAGG ATCTGCTCGG CTGCCTCAAG 
TACTTCATCA ACTTCTTCTT CTACAAATTT GGGCTGGAGA TCTGCTTCTT GATGGCCGTG 
AACGTGATTG GGCAGCGCAT GAACTTTATG GTGACCCTGC ACGGCTGCTG GCTGGTGGCC 
ATCCTCACCC GCAGACACCG CCAGGCCATC GCCCGCCTCT GGCCCAACTA CTGCCTCTTC 
CTGGCTCTGT TCCTGCTGTA CCAGTACCTG CTGTGTCTGG GGATGCCCCC GGCCCTGTGC 
ATCGATTACC CGTGGCGCTG GAGCCGGGCT GTCCCCATGA ACTCCGCACT CATCAAGTGG 
CTGTACCTGC CTGATTTCTT CCGGGCTCCC AACTCCACCA ACCTCATCAG TGACTTCCTC 
CTGCTGCTGT GCGCCTCCCA GCAGTGGCAA GTGTTCTCAG CTGAGCGCAC GGAGGAGTGG 
CAGTGCATGG CTGGTGTCAA CACCGACCGC CTGGAGCCGC TGCGGGGGGA GCCCAACCCT 
GTGCCCAACT TTATCCACTG CAGGTCCTAC CTCGACATGC TGAAGGTGGC CGTCTTCCGG 
TACCTGTTCT GGCTGGTGCT GGTGGTGGTG TTTGTCACGG GGGCCACCCG AATCAGCATC 
TTCGGGCTGG GCTACCTGCT GGCCTGCTTC TACCTGCTGC TCTTCGGCAC CGCCCTGCTG 
CAGAGGGACA CGCGGGCCCG CCTCGTGCTG TGGGACTGCC TCATTCTGTA CAACGTCACC 
GTCATCATCT CCAAGAACAT GCTGTCGCTC CTGGCCTGCG TCTTCGTGGA GCAGATGCAG 
ACCAGCTTCT GCTGGGTCAT CCAGCTCTTC AGCCTTGTGT GTACCGTCAA GGGCTACTAT 
GACCCCAAGG AGATGATGGG CAGAGACCAG GACTGCCTGC TGCCTGTGGA GGAGGCCGGC 
ATCATCTGGG ACAGCATCTG CTTCTTCTTC CTGCTGCTGC AGCGCCGTGT CTTCCTCAGC 
TATTACTACC TGCACGTCAG GGCCGACCTC CAGGCCACTG CCCTGCTGGC CTCCAGGGGC 
TTTGCCCTCT ACAACGCTGC CAACCTCAAG AGCATTGACT TCCACCGCAG GACAGAGGAG 
AAGTCCCTGG CCCAGCTGAA AAGACAGATG GAGCGTATCC GTGCCAAGCA GGAGAAGCAC 
AGGCAGGGCC GGGCGGACCG CGGTCACCCC CAGGACACCC TGGGCCCCAA GGACCCAGGC 
CTGGAGCCAG TCATCCACTC CGGGGACTAC TTCCTGTTTG AGTCTGACAG CGAGGAAGAG 
GAGGAGGCTG CCCCTGAAGA CCCTAGGCCA TCGGCCCAGA GTGCCTTCCA GATGGCATAC 
CAGGCATGGG TGACCAACGC CCAGACGGTG CTGAGGCGGC AGCAGCAGGA GCAGGCAAGG 
CAGAAGCAGG CAGGACAGCT GCCCACAGGA GGTGGCCCCA GCCAGGAGGT GGAGCCGGCA 
GAGGGCCCCG AGGAGGCAGC GGCAGGCCGG AGCCACATGG TGCAGAGGGT GCTGAGCACG 
GCGCAGTTCC TCTGGGTGCT GGGGCAGGCG CTGGTGGACG GGCTGACGCG CTGGCTGCAG 
GAGTTCACCC GGCACCACAG CACCATGAGT GACGTGCTGC GGGCAGAGCG CTACGTCCTC 
ACGCAGGAGC TCCTGCAGGG TGGTGAAGTG CACCGGGGCA TATTGGATCA GCTGTACACA 
AGCGAGGCTG AGGCCACACT GCCAGGCCCC ACCGAGGCCC CCGACGCACC AAGCACAGTG 
TCCAGTGGGC TGGGCGCGGA GGAGCCACTC AGCAGCATGA CAGACGACAC GGGCAGCCCC 
CTGAGCACTG GCTACCACAC GCGCAGTGGC AGCGAGGAGG TGGTCGCTGA CCCCGGGGAG 
CACGAGGCTG GCGCCTCTCT GCACCAGGGA CGCATGCGGA CGGCCAGCGA GCTGCTGCTG 
GACAGGCGCT TGCGCATCCC AGAGCTGGAG GAGGCAGAGC TGTTTGCGGA GGGGCAGGGC 
CGGGCGCTGC GGCTGCTGCG GGCCGTGTAC CAGTGCGTGG CTGCCCACTC AGAGCTGCTC 
TGCTACTTCA TCATCATCCT CAACCACATG GTCACGGCCT CTGCGGGCTC GCTGGTGCTG 
CCCGTGCTCG TCTTCCTGTG GGCCATGCTG TCCATCCCGC GGCCCAGCAA GCGCTTCTGG 
ATGACGGCCA TCGTCTTCAC CGAGGTCTCA GTGGTCGTCA AGTACCTGTT CCAGTTTGGA 
TTCTTCCCCT GGAACAGCCA CGTGGTGCTG CGGCGCTACG AGAACAAGCC CTACTTCCCG 
CCCCGCATCC TGGGCCTGGA GAAGACTGAC GGCTACATCA AGTACGACCT GGTGCAGCTC 
ATGGCCCTCT TCTTCCACCG CTCCCAGTTG CTGTGCTATG GCCTCTGGGA CCACGAGGAG 
GACTCACCAT CCAAGGAGCA TGACAAGAGC GGCAGGAAGG AGCAGGGGGC CGAGGAGGGG 
CCAGGGGCCC TGCCGGAACC CCAGGCCAAG GCAGGCACAG GGCTCGAGGA GGAGCCAGGA 
GTGCCCGAGG CCACCACCGA GGACCATGTT CAGGTGGAAG CAAGGGTTGT ACCGACGGAT 
GGGACCCCAG AACCCCAAGT GGAGCTCAGG CCCCGTGACA CGAGGCGCAT CAGTCTACGT 
TTGAGGAGGA GGAGGAAGGA GGGCCCAGCA CAGAGAGGAC TGGCTGCCAT CGAAGCTGAG 
GACAGGGAGG AAGAGAAAGA GGCCCCCACG GGGAGAGAGA AGAGGCCAAG CCGCGCTGGA 
AGAAGAGTGA GGGCGGCTGG GCGGCGGCTG CAGGGCTTCT GTCTGTCCCT GGCCCAGGGC 
ACATACCGGC CGCTACGGCG CTTCTTCCAT GACATCCTGC ACACCAAGTA CCGCGCAGCC 
ACCGACGTCT ATGCCCTCAT GTTCCTGGCC GACGTCGTCG ACTTCATCAT CATCATTTTT 
GGCTTCTGGG CCTTTGGGAA ACACTCAGCG GCCACGGATA TCACGTCCTC CCTATCAGAC 
GACCAGGTAC CCGAGGCCTT CCTGGTCATG CTGCTGATCC AGTTCAGTAC CATGGTGGTC 
GACCGCGCCC TCTACCTGCG CAAGACTGTG CTGGGCAAGC TGGCCTTCCA GGTGGCGCTG 
GTGCTGGCCA TCCACCTATG GATGTTCTTC ATCCTGCCTG CCGTCACTGA GAGGATGTTC 
AGCCAGAACG TGGTGGCCCA GCTGTGGTAC TTTGTGAAAT GCATCTACTT CGCCCTGTCC 
GCCTACCAGA TCCGCTGTGG CTACCCCACC CGCATCCTTG GCAACTTCCT CACCAAGAAG 
TACAATCACC TCAACCTCTT CCTTTTCCAG GGGTTCCGGC TGGTGCCGTT TCTGGTGGAG 
CTGCGGGCAG TGATGGACTG GGTGTGGACG GACACCACGC TGTCCCTGTC CAGCTGGATG 
TGCGTGGAGG ACATCTATGC CAACATCTTC ATCATCAAAT GCAGCCGAGA GACAGAAAAG 
AAATATCCAC AGCCCAAAGG GCAGAAGAAG AAGAAGATCG TCAAGTACGG CATGGGTGGC 
CTCATCATCC TCTTCCTCAT CGCCATCATC TGGTTCCCAC TGCTTTTCAT GTCACTGGTG 
CGTTCCGTGG TCGGGGTCGT CAACCAGCCC GTCGATGTCA CCGTCACCCT CAAGCTGGGC 
GGCTATGAGC CACTGTTCAC CATGAGCGCC CAGCAGCCGT CCATCATCCC CTTCACGGCC 
CAGGCCTACG AGGAGCTGTC CCGGCAGTTT GACCCTCACC CACTGGCCAT GCAGTTCATC 
AGCCAGTACA GCCCTGAGGA CATTGTCACG GCGCAGATTG AGGGCAGCTC CGGGGCGCTG 
TGGCGCATCA GCCCACCCAG CCGCGCCCAG ATGAAGCGAG AGCTCTACAA CGGCACGGCT 
GACATCACCC TGCGTTTCAC CTGGAACTTC CAGAGGGACC TGGCCAAGGG AGGCACCGTG 
GAGTACGCCA ACGAGAAGCA CATGCTGGCC CTGGCCCCCA ACAGCACCGC ACGGCGGCAG 
CTGGCCAGCC TGCTCGAGGG CACCTCGGAC CAGTCTGTGG TCATCCCAAA TCTCTTCCCC 
AAGTATATCC GTGCCCCCAA CGGGCCCGAA GCCAACCCTG TGAAGCAGCT GCAGCCCAAC 
GAGGAGGCCG ACTACCTCGG CGTGCGCATC CAGCTGCGGA GGGAGCAGGG TGCGGGGGCC 
ACCGGCTTCC TTGAATGGTG GGTCATCGAG CTGCAGGAGT GCCGGACCGA CTGCAACCTA 
CTGCCCATGG TCATCTTCAG CGACAAAGTC AGCCCACCGA GCCTCGGCTT CCTGGCTGGC 
TACGGGATCA TGGGGCTGTA TGTCTCCATC GTGCTGGTCA TCGGCAAGTT CGTGCGTGGA 
TTCTTCAGCG AGATCTCGCA CTCCATTATG TTCGAGGAGC TGCCGTGCGT GGACCGCATC 
CTCAAGCTCT GCCAGGACAT CTTCCTGGTG CGGGAGACTC GTGAGCTGGA GCTGGAGGAG 
GAGTTGTACG CCAAGCTCAT CTTCCTCTAC CGCTCACCAG AGACCATGAT CAAGTGGACT 
CGTGAGAAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007992533.1
CDS	270..7904
Translation	MEPHVLGAVLYWLLLPCALLAEASLPFLVVENPSSRSSLEASTASHTHIWHGRTFAGCLSCVLLKACLLRFSGLSLVYLLFLLLLPWFPGPTRHSLQGHTGRLLRALLGLSLLFLVAHLALQICLHTVPHLDQLLGPSCSRWETLSRHIGVTRLDLKDIPNAIRLVAPDLGILVVSSVCLGLCGRLARNTQQSPHMQELDDDERDVDASPPAGLQEAATLAPTRRSRLAARFRVTAHWLLVAAGRVLAVTLLALAGIAHPSALSSVYLLVFLAICTWWACHFPISTLGFNRLCVAVGCFGAGHVICLYCYQTPLAQALLPPAGIWARVLGLKDFVGPANCSSPHVLVLNTSLDWPMYASPGVLLLLCYATASLRKLRVHRPSGQRKEAAKGDETRELELAELDQWPQDGEAAQHVVPTAPDMEADNCIVHELTGQSPLRQRPVRPKRAEPREASPLHSLGHLIMDQSYVCALIAMMVWSITYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGMTLCCLRYVWAMDLRPELPTTLGPVSLRQLGLEHTRYPCLDLGAMLLYTLTFWLLLRQFVKEKLLKRAEAPAALMEVTVADTEPTRTQTLLQSLGELVKGIYAKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYSLWRKLLKAFWWLVVAYTMLVLIAVYTFQFQDFPAYWRNLTGFTDEQLGDLGLEQFSVSELFSSILVPGFFLLACILQLHYFHRPFMQLTDMEHVSPPRRRLPRWAHRQDAVSGTPLLQQQQQEEEEEEGPRDEGLGMATPHQATQVPEGAAKWGLVAERLLELAAGFSDVLSRVQVFLRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCVIIVCKMLYQLKVVNPQEYSSNCTEPSPNSTNLLTMEINQSLLYRGPVDPANWFGVRKGFPNLGYIQNHLQVLLLLVFEAIVYRRQEHHRRQHQLAPLPAQAVFASGTRQQLDQDLLGCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVTLHGCWLVAILTRRHRQAIARLWPNYCLFLALFLLYQYLLCLGMPPALCIDYPWRWSRAVPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQCMAGVNTDRLEPLRGEPNPVPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTALLQRDTRARLVLWDCLILYNVTVIISKNMLSLLACVFVEQMQTSFCWVIQLFSLVCTVKGYYDPKEMMGRDQDCLLPVEEAGIIWDSICFFFLLLQRRVFLSYYYLHVRADLQATALLASRGFALYNAANLKSIDFHRRTEEKSLAQLKRQMERIRAKQEKHRQGRADRGHPQDTLGPKDPGLEPVIHSGDYFLFESDSEEEEEAAPEDPRPSAQSAFQMAYQAWVTNAQTVLRRQQQEQARQKQAGQLPTGGGPSQEVEPAEGPEEAAAGRSHMVQRVLSTAQFLWVLGQALVDGLTRWLQEFTRHHSTMSDVLRAERYVLTQELLQGGEVHRGILDQLYTSEAEATLPGPTEAPDAPSTVSSGLGAEEPLSSMTDDTGSPLSTGYHTRSGSEEVVADPGEHEAGASLHQGRMRTASELLLDRRLRIPELEEAELFAEGQGRALRLLRAVYQCVAAHSELLCYFIIILNHMVTASAGSLVLPVLVFLWAMLSIPRPSKRFWMTAIVFTEVSVVVKYLFQFGFFPWNSHVVLRRYENKPYFPPRILGLEKTDGYIKYDLVQLMALFFHRSQLLCYGLWDHEEDSPSKEHDKSGRKEQGAEEGPGALPEPQAKAGTGLEEEPGVPEATTEDHVQVEARVVPTDGTPEPQVELRPRDTRRISLRLRRRRKEGPAQRGLAAIEAEDREEEKEAPTGREKRPSRAGRRVRAAGRRLQGFCLSLAQGTYRPLRRFFHDILHTKYRAATDVYALMFLADVVDFIIIIFGFWAFGKHSAATDITSSLSDDQVPEAFLVMLLIQFSTMVVDRALYLRKTVLGKLAFQVALVLAIHLWMFFILPAVTERMFSQNVVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSSWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLVRSVVGVVNQPVDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPHPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Target ORF information:

RefSeq Version	XM_007994342.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007994342.1

ORF Insert Sequence:

ATGGAACCGC ACGTGCTCGG CGCGGTCCTG TACTGGCTGC TGCTGCCCTG CGCGCTGCTG 
GCGGAAGCAA GTTTGCCCTT CCTTGTGGTA GAGAATCCTA GCTCACGGAG CAGTTTAGAA 
GCCAGCACCG CATCCCACAC ACACATCTGG CACGGACGGA CCTTTGCTGG GTGTCTGTCC 
TGTGTTCTTC TGAAAGCCTG CCTGCTCCGT TTCAGCGGGC TCTCGCTGGT CTACCTGCTC 
TTCCTGCTGC TGCTGCCCTG GTTCCCTGGC CCCACCCGGC ATAGCCTCCA AGGTCACACA 
GGCCGCCTCC TGCGGGCGCT GCTGGGCCTC AGCCTGCTCT TCCTGGTGGC CCATCTCGCT 
CTCCAGATCT GCCTGCACAC CGTGCCCCAC CTGGACCAGC TCCTGGGACC CAGCTGCAGC 
CGCTGGGAGA CCCTCTCGCG ACACATAGGG GTCACAAGGC TGGACCTGAA GGACATCCCC 
AACGCCATCC GGCTGGTGGC CCCTGACCTG GGCATCTTGG TGGTCTCCTC TGTCTGCCTC 
GGCCTCTGTG GGCGCCTCGC AAGGAACACC CAGCAGAGCC CACATATGCA GGAGCTGGAT 
GATGACGAGA GGGATGTGGA TGCCAGTCCC CCGGCAGGGC TGCAGGAAGC CGCAACGCTG 
GCCCCTACAC GGAGGTCACG GCTGGCCGCC CGTTTCCGCG TCACTGCCCA CTGGCTGCTG 
GTGGCGGCTG GGCGGGTCCT GGCCGTAACA CTACTCGCAC TGGCAGGCAT CGCCCACCCC 
TCGGCCCTCT CCAGCGTCTA CCTGCTGGTC TTCCTGGCCA TCTGCACCTG GTGGGCCTGC 
CACTTCCCCA TCAGCACGCT GGGCTTCAAC AGACTCTGCG TCGCAGTGGG CTGCTTCGGC 
GCCGGCCATG TCATCTGCCT CTACTGCTAC CAGACGCCCT TGGCCCAGGC TCTGCTCCCG 
CCTGCCGGCA TCTGGGCTAG GGTGCTGGGT CTCAAGGACT TTGTGGGTCC TGCGAACTGT 
TCCAGCCCCC ATGTGCTGGT CCTCAACACC AGCCTGGACT GGCCCATGTA TGCCAGCCCC 
GGCGTCCTGC TGCTGCTGTG CTACGCCACG GCCTCTCTGC GCAAGCTCCG TGTGCACCGC 
CCCTCCGGCC AGAGGAAGGA GGCGGCAAAG GGGGACGAGA CTCGGGAGCT GGAGCTGGCA 
GAGCTGGACC AGTGGCCCCA GGATGGGGAG GCTGCCCAGC ACGTGGTGCC CACAGCACCC 
GACATGGAGG CTGATAACTG CATCGTGCAC GAGCTGACAG GCCAGAGCCC CCTCCGGCAG 
CGGCCTGTGC GGCCCAAGCG GGCTGAGCCC AGGGAGGCAT CCCCACTCCA CAGCCTGGGC 
CACCTCATCA TGGACCAGAG CTATGTGTGC GCACTCATCG CCATGATGGT GTGGAGCATC 
ACCTACCACA GCTGGCTGAC CTTCGTGCTG CTGCTCTGGG CCTGCCTCAT CTGGACGGTG 
CGCAGCCGCC ACCAGCTGGC CATGCTGTGC TCGCCCTGCA TCCTGCTGTA TGGGATGACG 
CTGTGCTGTC TACGCTACGT GTGGGCCATG GACCTGCGCC CTGAGCTGCC CACCACTCTG 
GGCCCCGTCA GCCTGCGCCA GCTGGGGCTG GAGCACACCC GCTACCCCTG CCTGGACCTT 
GGCGCCATGT TGCTCTACAC CCTGACCTTC TGGCTCCTGC TGCGCCAGTT TGTGAAAGAG 
AAGCTGCTGA AGCGGGCAGA GGCTCCAGCT GCGCTAATGG AGGTCACTGT GGCAGACACA 
GAGCCCACAC GGACGCAGAC GCTTCTGCAG AGCCTGGGGG AGCTGGTGAA GGGCATCTAT 
GCCAAGTACT GGATCTATGT GTGTGCCGGC ATGTTCATCG TGGTCAGCTT CGCCGGCCGC 
CTCGTGGTCT ACAAGATTGT CTACATGTTC CTCTTCCTGC TCTGCCTCAC CCTCTTCCAG 
GTCTACTACA GCCTGTGGCG GAAGCTCCTC AAGGCCTTCT GGTGGCTCGT GGTGGCTTAC 
ACCATGCTGG TCCTCATCGC CGTCTACACC TTCCAGTTCC AGGACTTCCC CGCCTACTGG 
CGCAACCTCA CTGGCTTCAC CGATGAGCAG CTGGGGGACC TGGGCCTGGA GCAGTTCAGC 
GTGTCCGAGC TCTTCTCCAG CATCCTGGTG CCCGGCTTCT TCCTGCTGGC CTGTATCCTG 
CAGCTGCACT ACTTCCACCG GCCCTTTATG CAGCTCACCG ACATGGAGCA TGTGTCCCCG 
CCCCGCAGGC GCCTCCCTCG CTGGGCTCAC AGGCAGGATG CAGTGAGCGG GACCCCACTG 
CTGCAGCAGC AGCAGCAGGA GGAGGAGGAG GAGGAGGGCC CCAGGGATGA GGGGCTGGGC 
ATGGCCACTC CCCACCAGGC CACCCAGGTG CCTGAAGGCG CAGCCAAGTG GGGCCTGGTG 
GCCGAGCGGC TGCTGGAGCT GGCAGCTGGC TTTTCGGACG TCCTCTCACG GGTGCAGGTG 
TTCCTGCGGC GGCTGCTGGA GCTGCACGTC TTCAAGCTGG TGGCCCTGTA CACCGTCTGG 
GTGGCCCTGA AGGAGGTGTC AGTGATGAAC CTGCTGCTGG TGGTGCTGTG GGCCTTCGCC 
CTGCCCTACC CACGCTTCCG ACCCATGGCC TCCTGCCTGT CTACTGTGTG GACCTGTGTC 
ATCATCGTGT GTAAGATGCT GTACCAGCTC AAGGTCGTCA ACCCTCAGGA GTATTCCAGT 
AACTGCACCG AGCCCTCCCC CAACAGCACC AACCTGCTGA CCATGGAGAT CAACCAGTCC 
CTGCTGTACC GGGGGCCCGT GGATCCCGCC AACTGGTTTG GGGTGCGGAA AGGGTTCCCC 
AACCTGGGCT ACATCCAGAA CCACCTGCAG GTCCTGCTGC TGCTGGTATT CGAGGCCATC 
GTGTACCGGC GCCAGGAGCA CCACCGCCGG CAGCACCAGC TGGCCCCGCT GCCTGCCCAG 
GCCGTGTTCG CCAGCGGCAC CCGCCAGCAG CTGGACCAGG ATCTGCTCGG CTGCCTCAAG 
TACTTCATCA ACTTCTTCTT CTACAAATTT GGGCTGGAGA TCTGCTTCTT GATGGCCGTG 
AACGTGATTG GGCAGCGCAT GAACTTTATG GTGACCCTGC ACGGCTGCTG GCTGGTGGCC 
ATCCTCACCC GCAGACACCG CCAGGCCATC GCCCGCCTCT GGCCCAACTA CTGCCTCTTC 
CTGGCTCTGT TCCTGCTGTA CCAGTACCTG CTGTGTCTGG GGATGCCCCC GGCCCTGTGC 
ATCGATTACC CGTGGCGCTG GAGCCGGGCT GTCCCCATGA ACTCCGCACT CATCAAGTGG 
CTGTACCTGC CTGATTTCTT CCGGGCTCCC AACTCCACCA ACCTCATCAG TGACTTCCTC 
CTGCTGCTGT GCGCCTCCCA GCAGTGGCAA GTGTTCTCAG CTGAGCGCAC GGAGGAGTGG 
CAGTGCATGG CTGGTGTCAA CACCGACCGC CTGGAGCCGC TGCGGGGGGA GCCCAACCCT 
GTGCCCAACT TTATCCACTG CAGGTCCTAC CTCGACATGC TGAAGGTGGC CGTCTTCCGG 
TACCTGTTCT GGCTGGTGCT GGTGGTGGTG TTTGTCACGG GGGCCACCCG AATCAGCATC 
TTCGGGCTGG GCTACCTGCT GGCCTGCTTC TACCTGCTGC TCTTCGGCAC CGCCCTGCTG 
CAGAGGGACA CGCGGGCCCG CCTCGTGCTG TGGGACTGCC TCATTCTGTA CAACGTCACC 
GTCATCATCT CCAAGAACAT GCTGTCGCTC CTGGCCTGCG TCTTCGTGGA GCAGATGCAG 
ACCAGCTTCT GCTGGGTCAT CCAGCTCTTC AGCCTTGTGT GTACCGTCAA GGGCTACTAT 
GACCCCAAGG AGATGATGGG CAGAGACCAG GACTGCCTGC TGCCTGTGGA GGAGGCCGGC 
ATCATCTGGG ACAGCATCTG CTTCTTCTTC CTGCTGCTGC AGCGCCGTGT CTTCCTCAGC 
TATTACTACC TGCACGTCAG GGCCGACCTC CAGGCCACTG CCCTGCTGGC CTCCAGGGGC 
TTTGCCCTCT ACAACGCTGC CAACCTCAAG AGCATTGACT TCCACCGCAG GACAGAGGAG 
AAGTCCCTGG CCCAGCTGAA AAGACAGATG GAGCGTATCC GTGCCAAGCA GGAGAAGCAC 
AGGCAGGGCC GGGCGGACCG CGGTCACCCC CAGGACACCC TGGGCCCCAA GGACCCAGGC 
CTGGAGCCAG TCATCCACTC CGGGGACTAC TTCCTGTTTG AGTCTGACAG CGAGGAAGAG 
GAGGAGGCTG CCCCTGAAGA CCCTAGGCCA TCGGCCCAGA GTGCCTTCCA GATGGCATAC 
CAGGCATGGG TGACCAACGC CCAGACGGTG CTGAGGCGGC AGCAGCAGGA GCAGGCAAGG 
CAGAAGCAGG CAGGACAGCT GCCCACAGGA GGTGGCCCCA GCCAGGAGGT GGAGCCGGCA 
GAGGGCCCCG AGGAGGCAGC GGCAGGCCGG AGCCACATGG TGCAGAGGGT GCTGAGCACG 
GCGCAGTTCC TCTGGGTGCT GGGGCAGGCG CTGGTGGACG GGCTGACGCG CTGGCTGCAG 
GAGTTCACCC GGCACCACAG CACCATGAGT GACGTGCTGC GGGCAGAGCG CTACGTCCTC 
ACGCAGGAGC TCCTGCAGGG TGGTGAAGTG CACCGGGGCA TATTGGATCA GCTGTACACA 
AGCGAGGCTG AGGCCACACT GCCAGGCCCC ACCGAGGCCC CCGACGCACC AAGCACAGTG 
TCCAGTGGGC TGGGCGCGGA GGAGCCACTC AGCAGCATGA CAGACGACAC GGGCAGCCCC 
CTGAGCACTG GCTACCACAC GCGCAGTGGC AGCGAGGAGG TGGTCGCTGA CCCCGGGGAG 
CACGAGGCTG GCGCCTCTCT GCACCAGGGA CGCATGCGGA CGGCCAGCGA GCTGCTGCTG 
GACAGGCGCT TGCGCATCCC AGAGCTGGAG GAGGCAGAGC TGTTTGCGGA GGGGCAGGGC 
CGGGCGCTGC GGCTGCTGCG GGCCGTGTAC CAGTGCGTGG CTGCCCACTC AGAGCTGCTC 
TGCTACTTCA TCATCATCCT CAACCACATG GTCACGGCCT CTGCGGGCTC GCTGGTGCTG 
CCCGTGCTCG TCTTCCTGTG GGCCATGCTG TCCATCCCGC GGCCCAGCAA GCGCTTCTGG 
ATGACGGCCA TCGTCTTCAC CGAGGTCTCA GTGGTCGTCA AGTACCTGTT CCAGTTTGGA 
TTCTTCCCCT GGAACAGCCA CGTGGTGCTG CGGCGCTACG AGAACAAGCC CTACTTCCCG 
CCCCGCATCC TGGGCCTGGA GAAGACTGAC GGCTACATCA AGTACGACCT GGTGCAGCTC 
ATGGCCCTCT TCTTCCACCG CTCCCAGTTG CTGTGCTATG GCCTCTGGGA CCACGAGGAG 
GACTCACCAT CCAAGGAGCA TGACAAGAGC GGCAGGAAGG AGCAGGGGGC CGAGGAGGGG 
CCAGGGGCCC TGCCGGAACC CCAGGCCAAG GCAGGCACAG GGCTCGAGGA GGAGCCAGGA 
GTGCCCGAGG CCACCACCGA GGACCATGTT CAGGTGGAAG CAAGGGTTGT ACCGACGGAT 
GGGACCCCAG AACCCCAAGT GGAGCTCAGG CCCCGTGACA CGAGGCGCAT CAGTCTACGT 
TTGAGGAGGA GGAGGAAGGA GGGCCCAGCA CAGAGAGGAC TGGCTGCCAT CGAAGCTGAG 
GACAGGGAGG AAGAGAAAGA GGCCCCCACG GGGAGAGAGA AGAGGCCAAG CCGCGCTGGA 
AGAAGAGTGA GGGCGGCTGG GCGGCGGCTG CAGGGCTTCT GTCTGTCCCT GGCCCAGGGC 
ACATACCGGC CGCTACGGCG CTTCTTCCAT GACATCCTGC ACACCAAGTA CCGCGCAGCC 
ACCGACGTCT ATGCCCTCAT GTTCCTGGCC GACGTCGTCG ACTTCATCAT CATCATTTTT 
GGCTTCTGGG CCTTTGGGAA ACACTCAGCG GCCACGGATA TCACGTCCTC CCTATCAGAC 
GACCAGGTAC CCGAGGCCTT CCTGGTCATG CTGCTGATCC AGTTCAGTAC CATGGTGGTC 
GACCGCGCCC TCTACCTGCG CAAGACTGTG CTGGGCAAGC TGGCCTTCCA GGTGGCGCTG 
GTGCTGGCCA TCCACCTATG GATGTTCTTC ATCCTGCCTG CCGTCACTGA GAGGATGTTC 
AGCCAGAACG TGGTGGCCCA GCTGTGGTAC TTTGTGAAAT GCATCTACTT CGCCCTGTCC 
GCCTACCAGA TCCGCTGTGG CTACCCCACC CGCATCCTTG GCAACTTCCT CACCAAGAAG 
TACAATCACC TCAACCTCTT CCTTTTCCAG GGGTTCCGGC TGGTGCCGTT TCTGGTGGAG 
CTGCGGGCAG TGATGGACTG GGTGTGGACG GACACCACGC TGTCCCTGTC CAGCTGGATG 
TGCGTGGAGG ACATCTATGC CAACATCTTC ATCATCAAAT GCAGCCGAGA GACAGAAAAG 
AAATATCCAC AGCCCAAAGG GCAGAAGAAG AAGAAGATCG TCAAGTACGG CATGGGTGGC 
CTCATCATCC TCTTCCTCAT CGCCATCATC TGGTTCCCAC TGCTTTTCAT GTCACTGGTG 
CGTTCCGTGG TCGGGGTCGT CAACCAGCCC GTCGATGTCA CCGTCACCCT CAAGCTGGGC 
GGCTATGAGC CACTGTTCAC CATGAGCGCC CAGCAGCCGT CCATCATCCC CTTCACGGCC 
CAGGCCTACG AGGAGCTGTC CCGGCAGTTT GACCCTCACC CACTGGCCAT GCAGTTCATC 
AGCCAGTACA GCCCTGAGGA CATTGTCACG GCGCAGATTG AGGGCAGCTC CGGGGCGCTG 
TGGCGCATCA GCCCACCCAG CCGCGCCCAG ATGAAGCGAG AGCTCTACAA CGGCACGGCT 
GACATCACCC TGCGTTTCAC CTGGAACTTC CAGAGGGACC TGGCCAAGGG AGGCACCGTG 
GAGTACGCCA ACGAGAAGCA CATGCTGGCC CTGGCCCCCA ACAGCACCGC ACGGCGGCAG 
CTGGCCAGCC TGCTCGAGGG CACCTCGGAC CAGTCTGTGG TCATCCCAAA TCTCTTCCCC 
AAGTATATCC GTGCCCCCAA CGGGCCCGAA GCCAACCCTG TGAAGCAGCT GCAGCCCAAC 
GAGGAGGCCG ACTACCTCGG CGTGCGCATC CAGCTGCGGA GGGAGCAGGG TGCGGGGGCC 
ACCGGCTTCC TTGAATGGTG GGTCATCGAG CTGCAGGAGT GCCGGACCGA CTGCAACCTA 
CTGCCCATGG TCATCTTCAG CGACAAAGTC AGCCCACCGA GCCTCGGCTT CCTGGCTGGC 
TACGGGATCA TGGGGCTGTA TGTCTCCATC GTGCTGGTCA TCGGCAAGTT CGTGCGTGGA 
TTCTTCAGCG AGATCTCGCA CTCCATTATG TTCGAGGAGC TGCCGTGCGT GGACCGCATC 
CTCAAGCTCT GCCAGGACAT CTTCCTGGTG CGGGAGACTC GTGAGCTGGA GCTGGAGGAG 
GAGTTGTACG CCAAGCTCAT CTTCCTCTAC CGCTCACCAG AGACCATGAT CAAGTGGACT 
CGTGAGAAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO1 ( XM_007994343.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007992534.1 Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA.

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CloneID	OCh85934
Clone ID Related Accession (Same CDS sequence)	XM_007994343.1
Accession Version	XM_007994343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	piezo-type mechanosensitive ion channel component 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735242.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCGC ACGTGCTCGG CGCGGTCCTG TACTGGCTGC TGCTGCCCTG CGCGCTGCTG 
GCGGCCTGCC TGCTCCGTTT CAGCGGGCTC TCGCTGGTCT ACCTGCTCTT CCTGCTGCTG 
CTGCCCTGGT TCCCTGGCCC CACCCGGCAT AGCCTCCAAG GTCACACAGG CCGCCTCCTG 
CGGGCGCTGC TGGGCCTCAG CCTGCTCTTC CTGGTGGCCC ATCTCGCTCT CCAGATCTGC 
CTGCACACCG TGCCCCACCT GGACCAGCTC CTGGGACCCA GCTGCAGCCG CTGGGAGACC 
CTCTCGCGAC ACATAGGGGT CACAAGGCTG GACCTGAAGG ACATCCCCAA CGCCATCCGG 
CTGGTGGCCC CTGACCTGGG CATCTTGGTG GTCTCCTCTG TCTGCCTCGG CCTCTGTGGG 
CGCCTCGCAA GGAACACCCA GCAGAGCCCA CATATGCAGG AGCTGGATGA TGACGAGAGG 
GATGTGGATG CCAGTCCCCC GGCAGGGCTG CAGGAAGCCG CAACGCTGGC CCCTACACGG 
AGGTCACGGC TGGCCGCCCG TTTCCGCGTC ACTGCCCACT GGCTGCTGGT GGCGGCTGGG 
CGGGTCCTGG CCGTAACACT ACTCGCACTG GCAGGCATCG CCCACCCCTC GGCCCTCTCC 
AGCGTCTACC TGCTGGTCTT CCTGGCCATC TGCACCTGGT GGGCCTGCCA CTTCCCCATC 
AGCACGCTGG GCTTCAACAG ACTCTGCGTC GCAGTGGGCT GCTTCGGCGC CGGCCATGTC 
ATCTGCCTCT ACTGCTACCA GACGCCCTTG GCCCAGGCTC TGCTCCCGCC TGCCGGCATC 
TGGGCTAGGG TGCTGGGTCT CAAGGACTTT GTGGGTCCTG CGAACTGTTC CAGCCCCCAT 
GTGCTGGTCC TCAACACCAG CCTGGACTGG CCCATGTATG CCAGCCCCGG CGTCCTGCTG 
CTGCTGTGCT ACGCCACGGC CTCTCTGCGC AAGCTCCGTG TGCACCGCCC CTCCGGCCAG 
AGGAAGGAGG CGGCAAAGGG GGACGAGACT CGGGAGCTGG AGCTGGCAGA GCTGGACCAG 
TGGCCCCAGG ATGGGGAGGC TGCCCAGCAC GTGGTGCCCA CAGCACCCGA CATGGAGGCT 
GATAACTGCA TCGTGCACGA GCTGACAGGC CAGAGCCCCC TCCGGCAGCG GCCTGTGCGG 
CCCAAGCGGG CTGAGCCCAG GGAGGCATCC CCACTCCACA GCCTGGGCCA CCTCATCATG 
GACCAGAGCT ATGTGTGCGC ACTCATCGCC ATGATGGTGT GGAGCATCAC CTACCACAGC 
TGGCTGACCT TCGTGCTGCT GCTCTGGGCC TGCCTCATCT GGACGGTGCG CAGCCGCCAC 
CAGCTGGCCA TGCTGTGCTC GCCCTGCATC CTGCTGTATG GGATGACGCT GTGCTGTCTA 
CGCTACGTGT GGGCCATGGA CCTGCGCCCT GAGCTGCCCA CCACTCTGGG CCCCGTCAGC 
CTGCGCCAGC TGGGGCTGGA GCACACCCGC TACCCCTGCC TGGACCTTGG CGCCATGTTG 
CTCTACACCC TGACCTTCTG GCTCCTGCTG CGCCAGTTTG TGAAAGAGAA GCTGCTGAAG 
CGGGCAGAGG CTCCAGCTGC GCTAATGGAG GTCACTGTGG CAGACACAGA GCCCACACGG 
ACGCAGACGC TTCTGCAGAG CCTGGGGGAG CTGGTGAAGG GCATCTATGC CAAGTACTGG 
ATCTATGTGT GTGCCGGCAT GTTCATCGTG GTCAGCTTCG CCGGCCGCCT CGTGGTCTAC 
AAGATTGTCT ACATGTTCCT CTTCCTGCTC TGCCTCACCC TCTTCCAGGT CTACTACAGC 
CTGTGGCGGA AGCTCCTCAA GGCCTTCTGG TGGCTCGTGG TGGCTTACAC CATGCTGGTC 
CTCATCGCCG TCTACACCTT CCAGTTCCAG GACTTCCCCG CCTACTGGCG CAACCTCACT 
GGCTTCACCG ATGAGCAGCT GGGGGACCTG GGCCTGGAGC AGTTCAGCGT GTCCGAGCTC 
TTCTCCAGCA TCCTGGTGCC CGGCTTCTTC CTGCTGGCCT GTATCCTGCA GCTGCACTAC 
TTCCACCGGC CCTTTATGCA GCTCACCGAC ATGGAGCATG TGTCCCCGCC CCGCAGGCGC 
CTCCCTCGCT GGGCTCACAG GCAGGATGCA GTGAGCGGGA CCCCACTGCT GCAGCAGCAG 
CAGCAGGAGG AGGAGGAGGA GGAGGGCCCC AGGGATGAGG GGCTGGGCAT GGCCACTCCC 
CACCAGGCCA CCCAGGTGCC TGAAGGCGCA GCCAAGTGGG GCCTGGTGGC CGAGCGGCTG 
CTGGAGCTGG CAGCTGGCTT TTCGGACGTC CTCTCACGGG TGCAGGTGTT CCTGCGGCGG 
CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTCTGGGT GGCCCTGAAG 
GAGGTGTCAG TGATGAACCT GCTGCTGGTG GTGCTGTGGG CCTTCGCCCT GCCCTACCCA 
CGCTTCCGAC CCATGGCCTC CTGCCTGTCT ACTGTGTGGA CCTGTGTCAT CATCGTGTGT 
AAGATGCTGT ACCAGCTCAA GGTCGTCAAC CCTCAGGAGT ATTCCAGTAA CTGCACCGAG 
CCCTCCCCCA ACAGCACCAA CCTGCTGACC ATGGAGATCA ACCAGTCCCT GCTGTACCGG 
GGGCCCGTGG ATCCCGCCAA CTGGTTTGGG GTGCGGAAAG GGTTCCCCAA CCTGGGCTAC 
ATCCAGAACC ACCTGCAGGT CCTGCTGCTG CTGGTATTCG AGGCCATCGT GTACCGGCGC 
CAGGAGCACC ACCGCCGGCA GCACCAGCTG GCCCCGCTGC CTGCCCAGGC CGTGTTCGCC 
AGCGGCACCC GCCAGCAGCT GGACCAGGAT CTGCTCGGCT GCCTCAAGTA CTTCATCAAC 
TTCTTCTTCT ACAAATTTGG GCTGGAGATC TGCTTCTTGA TGGCCGTGAA CGTGATTGGG 
CAGCGCATGA ACTTTATGGT GACCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTCACCCGC 
AGACACCGCC AGGCCATCGC CCGCCTCTGG CCCAACTACT GCCTCTTCCT GGCTCTGTTC 
CTGCTGTACC AGTACCTGCT GTGTCTGGGG ATGCCCCCGG CCCTGTGCAT CGATTACCCG 
TGGCGCTGGA GCCGGGCTGT CCCCATGAAC TCCGCACTCA TCAAGTGGCT GTACCTGCCT 
GATTTCTTCC GGGCTCCCAA CTCCACCAAC CTCATCAGTG ACTTCCTCCT GCTGCTGTGC 
GCCTCCCAGC AGTGGCAAGT GTTCTCAGCT GAGCGCACGG AGGAGTGGCA GTGCATGGCT 
GGTGTCAACA CCGACCGCCT GGAGCCGCTG CGGGGGGAGC CCAACCCTGT GCCCAACTTT 
ATCCACTGCA GGTCCTACCT CGACATGCTG AAGGTGGCCG TCTTCCGGTA CCTGTTCTGG 
CTGGTGCTGG TGGTGGTGTT TGTCACGGGG GCCACCCGAA TCAGCATCTT CGGGCTGGGC 
TACCTGCTGG CCTGCTTCTA CCTGCTGCTC TTCGGCACCG CCCTGCTGCA GAGGGACACG 
CGGGCCCGCC TCGTGCTGTG GGACTGCCTC ATTCTGTACA ACGTCACCGT CATCATCTCC 
AAGAACATGC TGTCGCTCCT GGCCTGCGTC TTCGTGGAGC AGATGCAGAC CAGCTTCTGC 
TGGGTCATCC AGCTCTTCAG CCTTGTGTGT ACCGTCAAGG GCTACTATGA CCCCAAGGAG 
ATGATGGGCA GAGACCAGGA CTGCCTGCTG CCTGTGGAGG AGGCCGGCAT CATCTGGGAC 
AGCATCTGCT TCTTCTTCCT GCTGCTGCAG CGCCGTGTCT TCCTCAGCTA TTACTACCTG 
CACGTCAGGG CCGACCTCCA GGCCACTGCC CTGCTGGCCT CCAGGGGCTT TGCCCTCTAC 
AACGCTGCCA ACCTCAAGAG CATTGACTTC CACCGCAGGA CAGAGGAGAA GTCCCTGGCC 
CAGCTGAAAA GACAGATGGA GCGTATCCGT GCCAAGCAGG AGAAGCACAG GCAGGGCCGG 
GCGGACCGCG GTCACCCCCA GGACACCCTG GGCCCCAAGG ACCCAGGCCT GGAGCCAGGG 
CCCGACAGTC CAGGGGGCTC CTCCCCGCCA CGGAGGCAGT GGTGGCGGCC CTGGCTGGAC 
CACGCCACAG TCATCCACTC CGGGGACTAC TTCCTGTTTG AGTCTGACAG CGAGGAAGAG 
GAGGAGGCTG CCCCTGAAGA CCCTAGGCCA TCGGCCCAGA GTGCCTTCCA GATGGCATAC 
CAGGCATGGG TGACCAACGC CCAGACGGTG CTGAGGCGGC AGCAGCAGGA GCAGGCAAGG 
CAGAAGCAGG CAGGACAGCT GCCCACAGGA GGTGGCCCCA GCCAGGAGGT GGAGCCGGCA 
GAGGGCCCCG AGGAGGCAGC GGCAGGCCGG AGCCACATGG TGCAGAGGGT GCTGAGCACG 
GCGCAGTTCC TCTGGGTGCT GGGGCAGGCG CTGGTGGACG GGCTGACGCG CTGGCTGCAG 
GAGTTCACCC GGCACCACAG CACCATGAGT GACGTGCTGC GGGCAGAGCG CTACGTCCTC 
ACGCAGGAGC TCCTGCAGGG TGGTGAAGTG CACCGGGGCA TATTGGATCA GCTGTACACA 
AGCGAGGCTG AGGCCACACT GCCAGGCCCC ACCGAGGCCC CCGACGCACC AAGCACAGTG 
TCCAGTGGGC TGGGCGCGGA GGAGCCACTC AGCAGCATGA CAGACGACAC GGGCAGCCCC 
CTGAGCACTG GCTACCACAC GCGCAGTGGC AGCGAGGAGG TGGTCGCTGA CCCCGGGGAG 
CACGAGGCTG GCGCCTCTCT GCACCAGGGA CGCATGCGGA CGGCCAGCGA GCTGCTGCTG 
GACAGGCGCT TGCGCATCCC AGAGCTGGAG GAGGCAGAGC TGTTTGCGGA GGGGCAGGGC 
CGGGCGCTGC GGCTGCTGCG GGCCGTGTAC CAGTGCGTGG CTGCCCACTC AGAGCTGCTC 
TGCTACTTCA TCATCATCCT CAACCACATG GTCACGGCCT CTGCGGGCTC GCTGGTGCTG 
CCCGTGCTCG TCTTCCTGTG GGCCATGCTG TCCATCCCGC GGCCCAGCAA GCGCTTCTGG 
ATGACGGCCA TCGTCTTCAC CGAGGTCTCA GTGGTCGTCA AGTACCTGTT CCAGTTTGGA 
TTCTTCCCCT GGAACAGCCA CGTGGTGCTG CGGCGCTACG AGAACAAGCC CTACTTCCCG 
CCCCGCATCC TGGGCCTGGA GAAGACTGAC GGCTACATCA AGTACGACCT GGTGCAGCTC 
ATGGCCCTCT TCTTCCACCG CTCCCAGTTG CTGTGCTATG GCCTCTGGGA CCACGAGGAG 
GACTCACCAT CCAAGGAGCA TGACAAGAGC GGCAGGAAGG AGCAGGGGGC CGAGGAGGGG 
CCAGGGGCCC TGCCGGAACC CCAGGCCAAG GCAGGCACAG GGCTCGAGGA GGAGCCAGGA 
GTGCCCGAGG CCACCACCGA GGACCATGTT CAGGTGGAAG CAAGGGTTGT ACCGACGGAT 
GGGACCCCAG AACCCCAAGT GGAGCTCAGG CCCCGTGACA CGAGGCGCAT CAGTCTACGT 
TTGAGGAGGA GGAGGAAGGA GGGCCCAGCA CAGAGAGGAC TGGCTGCCAT CGAAGCTGAG 
GACAGGGAGG AAGAGAAAGA GGCCCCCACG GGGAGAGAGA AGAGGCCAAG CCGCGCTGGA 
AGAAGAGTGA GGGCGGCTGG GCGGCGGCTG CAGGGCTTCT GTCTGTCCCT GGCCCAGGGC 
ACATACCGGC CGCTACGGCG CTTCTTCCAT GACATCCTGC ACACCAAGTA CCGCGCAGCC 
ACCGACGTCT ATGCCCTCAT GTTCCTGGCC GACGTCGTCG ACTTCATCAT CATCATTTTT 
GGCTTCTGGG CCTTTGGGAA ACACTCAGCG GCCACGGATA TCACGTCCTC CCTATCAGAC 
GACCAGGTAC CCGAGGCCTT CCTGGTCATG CTGCTGATCC AGTTCAGTAC CATGGTGGTC 
GACCGCGCCC TCTACCTGCG CAAGACTGTG CTGGGCAAGC TGGCCTTCCA GGTGGCGCTG 
GTGCTGGCCA TCCACCTATG GATGTTCTTC ATCCTGCCTG CCGTCACTGA GAGGATGTTC 
AGCCAGAACG TGGTGGCCCA GCTGTGGTAC TTTGTGAAAT GCATCTACTT CGCCCTGTCC 
GCCTACCAGA TCCGCTGTGG CTACCCCACC CGCATCCTTG GCAACTTCCT CACCAAGAAG 
TACAATCACC TCAACCTCTT CCTTTTCCAG GGGTTCCGGC TGGTGCCGTT TCTGGTGGAG 
CTGCGGGCAG TGATGGACTG GGTGTGGACG GACACCACGC TGTCCCTGTC CAGCTGGATG 
TGCGTGGAGG ACATCTATGC CAACATCTTC ATCATCAAAT GCAGCCGAGA GACAGAAAAG 
AAATATCCAC AGCCCAAAGG GCAGAAGAAG AAGAAGATCG TCAAGTACGG CATGGGTGGC 
CTCATCATCC TCTTCCTCAT CGCCATCATC TGGTTCCCAC TGCTTTTCAT GTCACTGGTG 
CGTTCCGTGG TCGGGGTCGT CAACCAGCCC GTCGATGTCA CCGTCACCCT CAAGCTGGGC 
GGCTATGAGC CACTGTTCAC CATGAGCGCC CAGCAGCCGT CCATCATCCC CTTCACGGCC 
CAGGCCTACG AGGAGCTGTC CCGGCAGTTT GACCCTCACC CACTGGCCAT GCAGTTCATC 
AGCCAGTACA GCCCTGAGGA CATTGTCACG GCGCAGATTG AGGGCAGCTC CGGGGCGCTG 
TGGCGCATCA GCCCACCCAG CCGCGCCCAG ATGAAGCGAG AGCTCTACAA CGGCACGGCT 
GACATCACCC TGCGTTTCAC CTGGAACTTC CAGAGGGACC TGGCCAAGGG AGGCACCGTG 
GAGTACGCCA ACGAGAAGCA CATGCTGGCC CTGGCCCCCA ACAGCACCGC ACGGCGGCAG 
CTGGCCAGCC TGCTCGAGGG CACCTCGGAC CAGTCTGTGG TCATCCCAAA TCTCTTCCCC 
AAGTATATCC GTGCCCCCAA CGGGCCCGAA GCCAACCCTG TGAAGCAGCT GCAGCCCAAC 
GAGGAGGCCG ACTACCTCGG CGTGCGCATC CAGCTGCGGA GGGAGCAGGG TGCGGGGGCC 
ACCGGCTTCC TTGAATGGTG GGTCATCGAG CTGCAGGAGT GCCGGACCGA CTGCAACCTA 
CTGCCCATGG TCATCTTCAG CGACAAAGTC AGCCCACCGA GCCTCGGCTT CCTGGCTGGC 
TACGGGATCA TGGGGCTGTA TGTCTCCATC GTGCTGGTCA TCGGCAAGTT CGTGCGTGGA 
TTCTTCAGCG AGATCTCGCA CTCCATTATG TTCGAGGAGC TGCCGTGCGT GGACCGCATC 
CTCAAGCTCT GCCAGGACAT CTTCCTGGTG CGGGAGACTC GTGAGCTGGA GCTGGAGGAG 
GAGTTGTACG CCAAGCTCAT CTTCCTCTAC CGCTCACCAG AGACCATGAT CAAGTGGACT 
CGTGAGAAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007992534.1
CDS	270..7844
Translation	MEPHVLGAVLYWLLLPCALLAACLLRFSGLSLVYLLFLLLLPWFPGPTRHSLQGHTGRLLRALLGLSLLFLVAHLALQICLHTVPHLDQLLGPSCSRWETLSRHIGVTRLDLKDIPNAIRLVAPDLGILVVSSVCLGLCGRLARNTQQSPHMQELDDDERDVDASPPAGLQEAATLAPTRRSRLAARFRVTAHWLLVAAGRVLAVTLLALAGIAHPSALSSVYLLVFLAICTWWACHFPISTLGFNRLCVAVGCFGAGHVICLYCYQTPLAQALLPPAGIWARVLGLKDFVGPANCSSPHVLVLNTSLDWPMYASPGVLLLLCYATASLRKLRVHRPSGQRKEAAKGDETRELELAELDQWPQDGEAAQHVVPTAPDMEADNCIVHELTGQSPLRQRPVRPKRAEPREASPLHSLGHLIMDQSYVCALIAMMVWSITYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGMTLCCLRYVWAMDLRPELPTTLGPVSLRQLGLEHTRYPCLDLGAMLLYTLTFWLLLRQFVKEKLLKRAEAPAALMEVTVADTEPTRTQTLLQSLGELVKGIYAKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYSLWRKLLKAFWWLVVAYTMLVLIAVYTFQFQDFPAYWRNLTGFTDEQLGDLGLEQFSVSELFSSILVPGFFLLACILQLHYFHRPFMQLTDMEHVSPPRRRLPRWAHRQDAVSGTPLLQQQQQEEEEEEGPRDEGLGMATPHQATQVPEGAAKWGLVAERLLELAAGFSDVLSRVQVFLRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCVIIVCKMLYQLKVVNPQEYSSNCTEPSPNSTNLLTMEINQSLLYRGPVDPANWFGVRKGFPNLGYIQNHLQVLLLLVFEAIVYRRQEHHRRQHQLAPLPAQAVFASGTRQQLDQDLLGCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVTLHGCWLVAILTRRHRQAIARLWPNYCLFLALFLLYQYLLCLGMPPALCIDYPWRWSRAVPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQCMAGVNTDRLEPLRGEPNPVPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTALLQRDTRARLVLWDCLILYNVTVIISKNMLSLLACVFVEQMQTSFCWVIQLFSLVCTVKGYYDPKEMMGRDQDCLLPVEEAGIIWDSICFFFLLLQRRVFLSYYYLHVRADLQATALLASRGFALYNAANLKSIDFHRRTEEKSLAQLKRQMERIRAKQEKHRQGRADRGHPQDTLGPKDPGLEPGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEAAPEDPRPSAQSAFQMAYQAWVTNAQTVLRRQQQEQARQKQAGQLPTGGGPSQEVEPAEGPEEAAAGRSHMVQRVLSTAQFLWVLGQALVDGLTRWLQEFTRHHSTMSDVLRAERYVLTQELLQGGEVHRGILDQLYTSEAEATLPGPTEAPDAPSTVSSGLGAEEPLSSMTDDTGSPLSTGYHTRSGSEEVVADPGEHEAGASLHQGRMRTASELLLDRRLRIPELEEAELFAEGQGRALRLLRAVYQCVAAHSELLCYFIIILNHMVTASAGSLVLPVLVFLWAMLSIPRPSKRFWMTAIVFTEVSVVVKYLFQFGFFPWNSHVVLRRYENKPYFPPRILGLEKTDGYIKYDLVQLMALFFHRSQLLCYGLWDHEEDSPSKEHDKSGRKEQGAEEGPGALPEPQAKAGTGLEEEPGVPEATTEDHVQVEARVVPTDGTPEPQVELRPRDTRRISLRLRRRRKEGPAQRGLAAIEAEDREEEKEAPTGREKRPSRAGRRVRAAGRRLQGFCLSLAQGTYRPLRRFFHDILHTKYRAATDVYALMFLADVVDFIIIIFGFWAFGKHSAATDITSSLSDDQVPEAFLVMLLIQFSTMVVDRALYLRKTVLGKLAFQVALVLAIHLWMFFILPAVTERMFSQNVVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSSWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLVRSVVGVVNQPVDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPHPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Target ORF information:

RefSeq Version	XM_007994343.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007994343.1

ORF Insert Sequence:

ATGGAACCGC ACGTGCTCGG CGCGGTCCTG TACTGGCTGC TGCTGCCCTG CGCGCTGCTG 
GCGGCCTGCC TGCTCCGTTT CAGCGGGCTC TCGCTGGTCT ACCTGCTCTT CCTGCTGCTG 
CTGCCCTGGT TCCCTGGCCC CACCCGGCAT AGCCTCCAAG GTCACACAGG CCGCCTCCTG 
CGGGCGCTGC TGGGCCTCAG CCTGCTCTTC CTGGTGGCCC ATCTCGCTCT CCAGATCTGC 
CTGCACACCG TGCCCCACCT GGACCAGCTC CTGGGACCCA GCTGCAGCCG CTGGGAGACC 
CTCTCGCGAC ACATAGGGGT CACAAGGCTG GACCTGAAGG ACATCCCCAA CGCCATCCGG 
CTGGTGGCCC CTGACCTGGG CATCTTGGTG GTCTCCTCTG TCTGCCTCGG CCTCTGTGGG 
CGCCTCGCAA GGAACACCCA GCAGAGCCCA CATATGCAGG AGCTGGATGA TGACGAGAGG 
GATGTGGATG CCAGTCCCCC GGCAGGGCTG CAGGAAGCCG CAACGCTGGC CCCTACACGG 
AGGTCACGGC TGGCCGCCCG TTTCCGCGTC ACTGCCCACT GGCTGCTGGT GGCGGCTGGG 
CGGGTCCTGG CCGTAACACT ACTCGCACTG GCAGGCATCG CCCACCCCTC GGCCCTCTCC 
AGCGTCTACC TGCTGGTCTT CCTGGCCATC TGCACCTGGT GGGCCTGCCA CTTCCCCATC 
AGCACGCTGG GCTTCAACAG ACTCTGCGTC GCAGTGGGCT GCTTCGGCGC CGGCCATGTC 
ATCTGCCTCT ACTGCTACCA GACGCCCTTG GCCCAGGCTC TGCTCCCGCC TGCCGGCATC 
TGGGCTAGGG TGCTGGGTCT CAAGGACTTT GTGGGTCCTG CGAACTGTTC CAGCCCCCAT 
GTGCTGGTCC TCAACACCAG CCTGGACTGG CCCATGTATG CCAGCCCCGG CGTCCTGCTG 
CTGCTGTGCT ACGCCACGGC CTCTCTGCGC AAGCTCCGTG TGCACCGCCC CTCCGGCCAG 
AGGAAGGAGG CGGCAAAGGG GGACGAGACT CGGGAGCTGG AGCTGGCAGA GCTGGACCAG 
TGGCCCCAGG ATGGGGAGGC TGCCCAGCAC GTGGTGCCCA CAGCACCCGA CATGGAGGCT 
GATAACTGCA TCGTGCACGA GCTGACAGGC CAGAGCCCCC TCCGGCAGCG GCCTGTGCGG 
CCCAAGCGGG CTGAGCCCAG GGAGGCATCC CCACTCCACA GCCTGGGCCA CCTCATCATG 
GACCAGAGCT ATGTGTGCGC ACTCATCGCC ATGATGGTGT GGAGCATCAC CTACCACAGC 
TGGCTGACCT TCGTGCTGCT GCTCTGGGCC TGCCTCATCT GGACGGTGCG CAGCCGCCAC 
CAGCTGGCCA TGCTGTGCTC GCCCTGCATC CTGCTGTATG GGATGACGCT GTGCTGTCTA 
CGCTACGTGT GGGCCATGGA CCTGCGCCCT GAGCTGCCCA CCACTCTGGG CCCCGTCAGC 
CTGCGCCAGC TGGGGCTGGA GCACACCCGC TACCCCTGCC TGGACCTTGG CGCCATGTTG 
CTCTACACCC TGACCTTCTG GCTCCTGCTG CGCCAGTTTG TGAAAGAGAA GCTGCTGAAG 
CGGGCAGAGG CTCCAGCTGC GCTAATGGAG GTCACTGTGG CAGACACAGA GCCCACACGG 
ACGCAGACGC TTCTGCAGAG CCTGGGGGAG CTGGTGAAGG GCATCTATGC CAAGTACTGG 
ATCTATGTGT GTGCCGGCAT GTTCATCGTG GTCAGCTTCG CCGGCCGCCT CGTGGTCTAC 
AAGATTGTCT ACATGTTCCT CTTCCTGCTC TGCCTCACCC TCTTCCAGGT CTACTACAGC 
CTGTGGCGGA AGCTCCTCAA GGCCTTCTGG TGGCTCGTGG TGGCTTACAC CATGCTGGTC 
CTCATCGCCG TCTACACCTT CCAGTTCCAG GACTTCCCCG CCTACTGGCG CAACCTCACT 
GGCTTCACCG ATGAGCAGCT GGGGGACCTG GGCCTGGAGC AGTTCAGCGT GTCCGAGCTC 
TTCTCCAGCA TCCTGGTGCC CGGCTTCTTC CTGCTGGCCT GTATCCTGCA GCTGCACTAC 
TTCCACCGGC CCTTTATGCA GCTCACCGAC ATGGAGCATG TGTCCCCGCC CCGCAGGCGC 
CTCCCTCGCT GGGCTCACAG GCAGGATGCA GTGAGCGGGA CCCCACTGCT GCAGCAGCAG 
CAGCAGGAGG AGGAGGAGGA GGAGGGCCCC AGGGATGAGG GGCTGGGCAT GGCCACTCCC 
CACCAGGCCA CCCAGGTGCC TGAAGGCGCA GCCAAGTGGG GCCTGGTGGC CGAGCGGCTG 
CTGGAGCTGG CAGCTGGCTT TTCGGACGTC CTCTCACGGG TGCAGGTGTT CCTGCGGCGG 
CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTCTGGGT GGCCCTGAAG 
GAGGTGTCAG TGATGAACCT GCTGCTGGTG GTGCTGTGGG CCTTCGCCCT GCCCTACCCA 
CGCTTCCGAC CCATGGCCTC CTGCCTGTCT ACTGTGTGGA CCTGTGTCAT CATCGTGTGT 
AAGATGCTGT ACCAGCTCAA GGTCGTCAAC CCTCAGGAGT ATTCCAGTAA CTGCACCGAG 
CCCTCCCCCA ACAGCACCAA CCTGCTGACC ATGGAGATCA ACCAGTCCCT GCTGTACCGG 
GGGCCCGTGG ATCCCGCCAA CTGGTTTGGG GTGCGGAAAG GGTTCCCCAA CCTGGGCTAC 
ATCCAGAACC ACCTGCAGGT CCTGCTGCTG CTGGTATTCG AGGCCATCGT GTACCGGCGC 
CAGGAGCACC ACCGCCGGCA GCACCAGCTG GCCCCGCTGC CTGCCCAGGC CGTGTTCGCC 
AGCGGCACCC GCCAGCAGCT GGACCAGGAT CTGCTCGGCT GCCTCAAGTA CTTCATCAAC 
TTCTTCTTCT ACAAATTTGG GCTGGAGATC TGCTTCTTGA TGGCCGTGAA CGTGATTGGG 
CAGCGCATGA ACTTTATGGT GACCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTCACCCGC 
AGACACCGCC AGGCCATCGC CCGCCTCTGG CCCAACTACT GCCTCTTCCT GGCTCTGTTC 
CTGCTGTACC AGTACCTGCT GTGTCTGGGG ATGCCCCCGG CCCTGTGCAT CGATTACCCG 
TGGCGCTGGA GCCGGGCTGT CCCCATGAAC TCCGCACTCA TCAAGTGGCT GTACCTGCCT 
GATTTCTTCC GGGCTCCCAA CTCCACCAAC CTCATCAGTG ACTTCCTCCT GCTGCTGTGC 
GCCTCCCAGC AGTGGCAAGT GTTCTCAGCT GAGCGCACGG AGGAGTGGCA GTGCATGGCT 
GGTGTCAACA CCGACCGCCT GGAGCCGCTG CGGGGGGAGC CCAACCCTGT GCCCAACTTT 
ATCCACTGCA GGTCCTACCT CGACATGCTG AAGGTGGCCG TCTTCCGGTA CCTGTTCTGG 
CTGGTGCTGG TGGTGGTGTT TGTCACGGGG GCCACCCGAA TCAGCATCTT CGGGCTGGGC 
TACCTGCTGG CCTGCTTCTA CCTGCTGCTC TTCGGCACCG CCCTGCTGCA GAGGGACACG 
CGGGCCCGCC TCGTGCTGTG GGACTGCCTC ATTCTGTACA ACGTCACCGT CATCATCTCC 
AAGAACATGC TGTCGCTCCT GGCCTGCGTC TTCGTGGAGC AGATGCAGAC CAGCTTCTGC 
TGGGTCATCC AGCTCTTCAG CCTTGTGTGT ACCGTCAAGG GCTACTATGA CCCCAAGGAG 
ATGATGGGCA GAGACCAGGA CTGCCTGCTG CCTGTGGAGG AGGCCGGCAT CATCTGGGAC 
AGCATCTGCT TCTTCTTCCT GCTGCTGCAG CGCCGTGTCT TCCTCAGCTA TTACTACCTG 
CACGTCAGGG CCGACCTCCA GGCCACTGCC CTGCTGGCCT CCAGGGGCTT TGCCCTCTAC 
AACGCTGCCA ACCTCAAGAG CATTGACTTC CACCGCAGGA CAGAGGAGAA GTCCCTGGCC 
CAGCTGAAAA GACAGATGGA GCGTATCCGT GCCAAGCAGG AGAAGCACAG GCAGGGCCGG 
GCGGACCGCG GTCACCCCCA GGACACCCTG GGCCCCAAGG ACCCAGGCCT GGAGCCAGGG 
CCCGACAGTC CAGGGGGCTC CTCCCCGCCA CGGAGGCAGT GGTGGCGGCC CTGGCTGGAC 
CACGCCACAG TCATCCACTC CGGGGACTAC TTCCTGTTTG AGTCTGACAG CGAGGAAGAG 
GAGGAGGCTG CCCCTGAAGA CCCTAGGCCA TCGGCCCAGA GTGCCTTCCA GATGGCATAC 
CAGGCATGGG TGACCAACGC CCAGACGGTG CTGAGGCGGC AGCAGCAGGA GCAGGCAAGG 
CAGAAGCAGG CAGGACAGCT GCCCACAGGA GGTGGCCCCA GCCAGGAGGT GGAGCCGGCA 
GAGGGCCCCG AGGAGGCAGC GGCAGGCCGG AGCCACATGG TGCAGAGGGT GCTGAGCACG 
GCGCAGTTCC TCTGGGTGCT GGGGCAGGCG CTGGTGGACG GGCTGACGCG CTGGCTGCAG 
GAGTTCACCC GGCACCACAG CACCATGAGT GACGTGCTGC GGGCAGAGCG CTACGTCCTC 
ACGCAGGAGC TCCTGCAGGG TGGTGAAGTG CACCGGGGCA TATTGGATCA GCTGTACACA 
AGCGAGGCTG AGGCCACACT GCCAGGCCCC ACCGAGGCCC CCGACGCACC AAGCACAGTG 
TCCAGTGGGC TGGGCGCGGA GGAGCCACTC AGCAGCATGA CAGACGACAC GGGCAGCCCC 
CTGAGCACTG GCTACCACAC GCGCAGTGGC AGCGAGGAGG TGGTCGCTGA CCCCGGGGAG 
CACGAGGCTG GCGCCTCTCT GCACCAGGGA CGCATGCGGA CGGCCAGCGA GCTGCTGCTG 
GACAGGCGCT TGCGCATCCC AGAGCTGGAG GAGGCAGAGC TGTTTGCGGA GGGGCAGGGC 
CGGGCGCTGC GGCTGCTGCG GGCCGTGTAC CAGTGCGTGG CTGCCCACTC AGAGCTGCTC 
TGCTACTTCA TCATCATCCT CAACCACATG GTCACGGCCT CTGCGGGCTC GCTGGTGCTG 
CCCGTGCTCG TCTTCCTGTG GGCCATGCTG TCCATCCCGC GGCCCAGCAA GCGCTTCTGG 
ATGACGGCCA TCGTCTTCAC CGAGGTCTCA GTGGTCGTCA AGTACCTGTT CCAGTTTGGA 
TTCTTCCCCT GGAACAGCCA CGTGGTGCTG CGGCGCTACG AGAACAAGCC CTACTTCCCG 
CCCCGCATCC TGGGCCTGGA GAAGACTGAC GGCTACATCA AGTACGACCT GGTGCAGCTC 
ATGGCCCTCT TCTTCCACCG CTCCCAGTTG CTGTGCTATG GCCTCTGGGA CCACGAGGAG 
GACTCACCAT CCAAGGAGCA TGACAAGAGC GGCAGGAAGG AGCAGGGGGC CGAGGAGGGG 
CCAGGGGCCC TGCCGGAACC CCAGGCCAAG GCAGGCACAG GGCTCGAGGA GGAGCCAGGA 
GTGCCCGAGG CCACCACCGA GGACCATGTT CAGGTGGAAG CAAGGGTTGT ACCGACGGAT 
GGGACCCCAG AACCCCAAGT GGAGCTCAGG CCCCGTGACA CGAGGCGCAT CAGTCTACGT 
TTGAGGAGGA GGAGGAAGGA GGGCCCAGCA CAGAGAGGAC TGGCTGCCAT CGAAGCTGAG 
GACAGGGAGG AAGAGAAAGA GGCCCCCACG GGGAGAGAGA AGAGGCCAAG CCGCGCTGGA 
AGAAGAGTGA GGGCGGCTGG GCGGCGGCTG CAGGGCTTCT GTCTGTCCCT GGCCCAGGGC 
ACATACCGGC CGCTACGGCG CTTCTTCCAT GACATCCTGC ACACCAAGTA CCGCGCAGCC 
ACCGACGTCT ATGCCCTCAT GTTCCTGGCC GACGTCGTCG ACTTCATCAT CATCATTTTT 
GGCTTCTGGG CCTTTGGGAA ACACTCAGCG GCCACGGATA TCACGTCCTC CCTATCAGAC 
GACCAGGTAC CCGAGGCCTT CCTGGTCATG CTGCTGATCC AGTTCAGTAC CATGGTGGTC 
GACCGCGCCC TCTACCTGCG CAAGACTGTG CTGGGCAAGC TGGCCTTCCA GGTGGCGCTG 
GTGCTGGCCA TCCACCTATG GATGTTCTTC ATCCTGCCTG CCGTCACTGA GAGGATGTTC 
AGCCAGAACG TGGTGGCCCA GCTGTGGTAC TTTGTGAAAT GCATCTACTT CGCCCTGTCC 
GCCTACCAGA TCCGCTGTGG CTACCCCACC CGCATCCTTG GCAACTTCCT CACCAAGAAG 
TACAATCACC TCAACCTCTT CCTTTTCCAG GGGTTCCGGC TGGTGCCGTT TCTGGTGGAG 
CTGCGGGCAG TGATGGACTG GGTGTGGACG GACACCACGC TGTCCCTGTC CAGCTGGATG 
TGCGTGGAGG ACATCTATGC CAACATCTTC ATCATCAAAT GCAGCCGAGA GACAGAAAAG 
AAATATCCAC AGCCCAAAGG GCAGAAGAAG AAGAAGATCG TCAAGTACGG CATGGGTGGC 
CTCATCATCC TCTTCCTCAT CGCCATCATC TGGTTCCCAC TGCTTTTCAT GTCACTGGTG 
CGTTCCGTGG TCGGGGTCGT CAACCAGCCC GTCGATGTCA CCGTCACCCT CAAGCTGGGC 
GGCTATGAGC CACTGTTCAC CATGAGCGCC CAGCAGCCGT CCATCATCCC CTTCACGGCC 
CAGGCCTACG AGGAGCTGTC CCGGCAGTTT GACCCTCACC CACTGGCCAT GCAGTTCATC 
AGCCAGTACA GCCCTGAGGA CATTGTCACG GCGCAGATTG AGGGCAGCTC CGGGGCGCTG 
TGGCGCATCA GCCCACCCAG CCGCGCCCAG ATGAAGCGAG AGCTCTACAA CGGCACGGCT 
GACATCACCC TGCGTTTCAC CTGGAACTTC CAGAGGGACC TGGCCAAGGG AGGCACCGTG 
GAGTACGCCA ACGAGAAGCA CATGCTGGCC CTGGCCCCCA ACAGCACCGC ACGGCGGCAG 
CTGGCCAGCC TGCTCGAGGG CACCTCGGAC CAGTCTGTGG TCATCCCAAA TCTCTTCCCC 
AAGTATATCC GTGCCCCCAA CGGGCCCGAA GCCAACCCTG TGAAGCAGCT GCAGCCCAAC 
GAGGAGGCCG ACTACCTCGG CGTGCGCATC CAGCTGCGGA GGGAGCAGGG TGCGGGGGCC 
ACCGGCTTCC TTGAATGGTG GGTCATCGAG CTGCAGGAGT GCCGGACCGA CTGCAACCTA 
CTGCCCATGG TCATCTTCAG CGACAAAGTC AGCCCACCGA GCCTCGGCTT CCTGGCTGGC 
TACGGGATCA TGGGGCTGTA TGTCTCCATC GTGCTGGTCA TCGGCAAGTT CGTGCGTGGA 
TTCTTCAGCG AGATCTCGCA CTCCATTATG TTCGAGGAGC TGCCGTGCGT GGACCGCATC 
CTCAAGCTCT GCCAGGACAT CTTCCTGGTG CGGGAGACTC GTGAGCTGGA GCTGGAGGAG 
GAGTTGTACG CCAAGCTCAT CTTCCTCTAC CGCTCACCAG AGACCATGAT CAAGTGGACT 
CGTGAGAAGG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO1 ( XM_007994344.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007992535.1 Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X4, mRNA.

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CloneID	OCh85935
Clone ID Related Accession (Same CDS sequence)	XM_007994344.1
Accession Version	XM_007994344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	piezo-type mechanosensitive ion channel component 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735242.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAT TGTCTGGGGC CACGCGGTGG TGGGAACCTG CAGTGCTGGG CAGTGGGTCT 
GGCTGGAGAG CCGCTGCAGA CAGGGATGAT GACGAGAGGG ATGTGGATGC CAGTCCCCCG 
GCAGGGCTGC AGGAAGCCGC AACGCTGGCC CCTACACGGA GGTCACGGCT GGCCGCCCGT 
TTCCGCGTCA CTGCCCACTG GCTGCTGGTG GCGGCTGGGC GGGTCCTGGC CGTAACACTA 
CTCGCACTGG CAGGCATCGC CCACCCCTCG GCCCTCTCCA GCGTCTACCT GCTGGTCTTC 
CTGGCCATCT GCACCTGGTG GGCCTGCCAC TTCCCCATCA GCACGCTGGG CTTCAACAGA 
CTCTGCGTCG CAGTGGGCTG CTTCGGCGCC GGCCATGTCA TCTGCCTCTA CTGCTACCAG 
ACGCCCTTGG CCCAGGCTCT GCTCCCGCCT GCCGGCATCT GGGCTAGGGT GCTGGGTCTC 
AAGGACTTTG TGGGTCCTGC GAACTGTTCC AGCCCCCATG TGCTGGTCCT CAACACCAGC 
CTGGACTGGC CCATGTATGC CAGCCCCGGC GTCCTGCTGC TGCTGTGCTA CGCCACGGCC 
TCTCTGCGCA AGCTCCGTGT GCACCGCCCC TCCGGCCAGA GGAAGGAGGC GGCAAAGGGG 
GACGAGACTC GGGAGCTGGA GCTGGCAGAG CTGGACCAGT GGCCCCAGGA TGGGGAGGCT 
GCCCAGCACG TGGTGCCCAC AGCACCCGAC ATGGAGGCTG ATAACTGCAT CGTGCACGAG 
CTGACAGGCC AGAGCCCCCT CCGGCAGCGG CCTGTGCGGC CCAAGCGGGC TGAGCCCAGG 
GAGGCATCCC CACTCCACAG CCTGGGCCAC CTCATCATGG ACCAGAGCTA TGTGTGCGCA 
CTCATCGCCA TGATGGTGTG GAGCATCACC TACCACAGCT GGCTGACCTT CGTGCTGCTG 
CTCTGGGCCT GCCTCATCTG GACGGTGCGC AGCCGCCACC AGCTGGCCAT GCTGTGCTCG 
CCCTGCATCC TGCTGTATGG GATGACGCTG TGCTGTCTAC GCTACGTGTG GGCCATGGAC 
CTGCGCCCTG AGCTGCCCAC CACTCTGGGC CCCGTCAGCC TGCGCCAGCT GGGGCTGGAG 
CACACCCGCT ACCCCTGCCT GGACCTTGGC GCCATGTTGC TCTACACCCT GACCTTCTGG 
CTCCTGCTGC GCCAGTTTGT GAAAGAGAAG CTGCTGAAGC GGGCAGAGGC TCCAGCTGCG 
CTAATGGAGG TCACTGTGGC AGACACAGAG CCCACACGGA CGCAGACGCT TCTGCAGAGC 
CTGGGGGAGC TGGTGAAGGG CATCTATGCC AAGTACTGGA TCTATGTGTG TGCCGGCATG 
TTCATCGTGG TCAGCTTCGC CGGCCGCCTC GTGGTCTACA AGATTGTCTA CATGTTCCTC 
TTCCTGCTCT GCCTCACCCT CTTCCAGGTC TACTACAGCC TGTGGCGGAA GCTCCTCAAG 
GCCTTCTGGT GGCTCGTGGT GGCTTACACC ATGCTGGTCC TCATCGCCGT CTACACCTTC 
CAGTTCCAGG ACTTCCCCGC CTACTGGCGC AACCTCACTG GCTTCACCGA TGAGCAGCTG 
GGGGACCTGG GCCTGGAGCA GTTCAGCGTG TCCGAGCTCT TCTCCAGCAT CCTGGTGCCC 
GGCTTCTTCC TGCTGGCCTG TATCCTGCAG CTGCACTACT TCCACCGGCC CTTTATGCAG 
CTCACCGACA TGGAGCATGT GTCCCCGCCC CGCAGGCGCC TCCCTCGCTG GGCTCACAGG 
CAGGATGCAG TGAGCGGGAC CCCACTGCTG CAGCAGCAGC AGCAGGAGGA GGAGGAGGAG 
GAGGGCCCCA GGGATGAGGG GCTGGGCATG GCCACTCCCC ACCAGGCCAC CCAGGTGCCT 
GAAGGCGCAG CCAAGTGGGG CCTGGTGGCC GAGCGGCTGC TGGAGCTGGC AGCTGGCTTT 
TCGGACGTCC TCTCACGGGT GCAGGTGTTC CTGCGGCGGC TGCTGGAGCT GCACGTCTTC 
AAGCTGGTGG CCCTGTACAC CGTCTGGGTG GCCCTGAAGG AGGTGTCAGT GATGAACCTG 
CTGCTGGTGG TGCTGTGGGC CTTCGCCCTG CCCTACCCAC GCTTCCGACC CATGGCCTCC 
TGCCTGTCTA CTGTGTGGAC CTGTGTCATC ATCGTGTGTA AGATGCTGTA CCAGCTCAAG 
GTCGTCAACC CTCAGGAGTA TTCCAGTAAC TGCACCGAGC CCTCCCCCAA CAGCACCAAC 
CTGCTGACCA TGGAGATCAA CCAGTCCCTG CTGTACCGGG GGCCCGTGGA TCCCGCCAAC 
TGGTTTGGGG TGCGGAAAGG GTTCCCCAAC CTGGGCTACA TCCAGAACCA CCTGCAGGTC 
CTGCTGCTGC TGGTATTCGA GGCCATCGTG TACCGGCGCC AGGAGCACCA CCGCCGGCAG 
CACCAGCTGG CCCCGCTGCC TGCCCAGGCC GTGTTCGCCA GCGGCACCCG CCAGCAGCTG 
GACCAGGATC TGCTCGGCTG CCTCAAGTAC TTCATCAACT TCTTCTTCTA CAAATTTGGG 
CTGGAGATCT GCTTCTTGAT GGCCGTGAAC GTGATTGGGC AGCGCATGAA CTTTATGGTG 
ACCCTGCACG GCTGCTGGCT GGTGGCCATC CTCACCCGCA GACACCGCCA GGCCATCGCC 
CGCCTCTGGC CCAACTACTG CCTCTTCCTG GCTCTGTTCC TGCTGTACCA GTACCTGCTG 
TGTCTGGGGA TGCCCCCGGC CCTGTGCATC GATTACCCGT GGCGCTGGAG CCGGGCTGTC 
CCCATGAACT CCGCACTCAT CAAGTGGCTG TACCTGCCTG ATTTCTTCCG GGCTCCCAAC 
TCCACCAACC TCATCAGTGA CTTCCTCCTG CTGCTGTGCG CCTCCCAGCA GTGGCAAGTG 
TTCTCAGCTG AGCGCACGGA GGAGTGGCAG TGCATGGCTG GTGTCAACAC CGACCGCCTG 
GAGCCGCTGC GGGGGGAGCC CAACCCTGTG CCCAACTTTA TCCACTGCAG GTCCTACCTC 
GACATGCTGA AGGTGGCCGT CTTCCGGTAC CTGTTCTGGC TGGTGCTGGT GGTGGTGTTT 
GTCACGGGGG CCACCCGAAT CAGCATCTTC GGGCTGGGCT ACCTGCTGGC CTGCTTCTAC 
CTGCTGCTCT TCGGCACCGC CCTGCTGCAG AGGGACACGC GGGCCCGCCT CGTGCTGTGG 
GACTGCCTCA TTCTGTACAA CGTCACCGTC ATCATCTCCA AGAACATGCT GTCGCTCCTG 
GCCTGCGTCT TCGTGGAGCA GATGCAGACC AGCTTCTGCT GGGTCATCCA GCTCTTCAGC 
CTTGTGTGTA CCGTCAAGGG CTACTATGAC CCCAAGGAGA TGATGGGCAG AGACCAGGAC 
TGCCTGCTGC CTGTGGAGGA GGCCGGCATC ATCTGGGACA GCATCTGCTT CTTCTTCCTG 
CTGCTGCAGC GCCGTGTCTT CCTCAGCTAT TACTACCTGC ACGTCAGGGC CGACCTCCAG 
GCCACTGCCC TGCTGGCCTC CAGGGGCTTT GCCCTCTACA ACGCTGCCAA CCTCAAGAGC 
ATTGACTTCC ACCGCAGGAC AGAGGAGAAG TCCCTGGCCC AGCTGAAAAG ACAGATGGAG 
CGTATCCGTG CCAAGCAGGA GAAGCACAGG CAGGGCCGGG CGGACCGCGG TCACCCCCAG 
GACACCCTGG GCCCCAAGGA CCCAGGCCTG GAGCCAGGGC CCGACAGTCC AGGGGGCTCC 
TCCCCGCCAC GGAGGCAGTG GTGGCGGCCC TGGCTGGACC ACGCCACAGT CATCCACTCC 
GGGGACTACT TCCTGTTTGA GTCTGACAGC GAGGAAGAGG AGGAGGCTGC CCCTGAAGAC 
CCTAGGCCAT CGGCCCAGAG TGCCTTCCAG ATGGCATACC AGGCATGGGT GACCAACGCC 
CAGACGGTGC TGAGGCGGCA GCAGCAGGAG CAGGCAAGGC AGAAGCAGGC AGGACAGCTG 
CCCACAGGAG GTGGCCCCAG CCAGGAGGTG GAGCCGGCAG AGGGCCCCGA GGAGGCAGCG 
GCAGGCCGGA GCCACATGGT GCAGAGGGTG CTGAGCACGG CGCAGTTCCT CTGGGTGCTG 
GGGCAGGCGC TGGTGGACGG GCTGACGCGC TGGCTGCAGG AGTTCACCCG GCACCACAGC 
ACCATGAGTG ACGTGCTGCG GGCAGAGCGC TACGTCCTCA CGCAGGAGCT CCTGCAGGGT 
GGTGAAGTGC ACCGGGGCAT ATTGGATCAG CTGTACACAA GCGAGGCTGA GGCCACACTG 
CCAGGCCCCA CCGAGGCCCC CGACGCACCA AGCACAGTGT CCAGTGGGCT GGGCGCGGAG 
GAGCCACTCA GCAGCATGAC AGACGACACG GGCAGCCCCC TGAGCACTGG CTACCACACG 
CGCAGTGGCA GCGAGGAGGT GGTCGCTGAC CCCGGGGAGC ACGAGGCTGG CGCCTCTCTG 
CACCAGGGAC GCATGCGGAC GGCCAGCGAG CTGCTGCTGG ACAGGCGCTT GCGCATCCCA 
GAGCTGGAGG AGGCAGAGCT GTTTGCGGAG GGGCAGGGCC GGGCGCTGCG GCTGCTGCGG 
GCCGTGTACC AGTGCGTGGC TGCCCACTCA GAGCTGCTCT GCTACTTCAT CATCATCCTC 
AACCACATGG TCACGGCCTC TGCGGGCTCG CTGGTGCTGC CCGTGCTCGT CTTCCTGTGG 
GCCATGCTGT CCATCCCGCG GCCCAGCAAG CGCTTCTGGA TGACGGCCAT CGTCTTCACC 
GAGGTCTCAG TGGTCGTCAA GTACCTGTTC CAGTTTGGAT TCTTCCCCTG GAACAGCCAC 
GTGGTGCTGC GGCGCTACGA GAACAAGCCC TACTTCCCGC CCCGCATCCT GGGCCTGGAG 
AAGACTGACG GCTACATCAA GTACGACCTG GTGCAGCTCA TGGCCCTCTT CTTCCACCGC 
TCCCAGTTGC TGTGCTATGG CCTCTGGGAC CACGAGGAGG ACTCACCATC CAAGGAGCAT 
GACAAGAGCG GCAGGAAGGA GCAGGGGGCC GAGGAGGGGC CAGGGGCCCT GCCGGAACCC 
CAGGCCAAGG CAGGCACAGG GCTCGAGGAG GAGCCAGGAG TGCCCGAGGC CACCACCGAG 
GACCATGTTC AGGTGGAAGC AAGGGTTGTA CCGACGGATG GGACCCCAGA ACCCCAAGTG 
GAGCTCAGGC CCCGTGACAC GAGGCGCATC AGTCTACGTT TGAGGAGGAG GAGGAAGGAG 
GGCCCAGCAC AGAGAGGACT GGCTGCCATC GAAGCTGAGG ACAGGGAGGA AGAGAAAGAG 
GCCCCCACGG GGAGAGAGAA GAGGCCAAGC CGCGCTGGAA GAAGAGTGAG GGCGGCTGGG 
CGGCGGCTGC AGGGCTTCTG TCTGTCCCTG GCCCAGGGCA CATACCGGCC GCTACGGCGC 
TTCTTCCATG ACATCCTGCA CACCAAGTAC CGCGCAGCCA CCGACGTCTA TGCCCTCATG 
TTCCTGGCCG ACGTCGTCGA CTTCATCATC ATCATTTTTG GCTTCTGGGC CTTTGGGAAA 
CACTCAGCGG CCACGGATAT CACGTCCTCC CTATCAGACG ACCAGGTACC CGAGGCCTTC 
CTGGTCATGC TGCTGATCCA GTTCAGTACC ATGGTGGTCG ACCGCGCCCT CTACCTGCGC 
AAGACTGTGC TGGGCAAGCT GGCCTTCCAG GTGGCGCTGG TGCTGGCCAT CCACCTATGG 
ATGTTCTTCA TCCTGCCTGC CGTCACTGAG AGGATGTTCA GCCAGAACGT GGTGGCCCAG 
CTGTGGTACT TTGTGAAATG CATCTACTTC GCCCTGTCCG CCTACCAGAT CCGCTGTGGC 
TACCCCACCC GCATCCTTGG CAACTTCCTC ACCAAGAAGT ACAATCACCT CAACCTCTTC 
CTTTTCCAGG GGTTCCGGCT GGTGCCGTTT CTGGTGGAGC TGCGGGCAGT GATGGACTGG 
GTGTGGACGG ACACCACGCT GTCCCTGTCC AGCTGGATGT GCGTGGAGGA CATCTATGCC 
AACATCTTCA TCATCAAATG CAGCCGAGAG ACAGAAAAGA AATATCCACA GCCCAAAGGG 
CAGAAGAAGA AGAAGATCGT CAAGTACGGC ATGGGTGGCC TCATCATCCT CTTCCTCATC 
GCCATCATCT GGTTCCCACT GCTTTTCATG TCACTGGTGC GTTCCGTGGT CGGGGTCGTC 
AACCAGCCCG TCGATGTCAC CGTCACCCTC AAGCTGGGCG GCTATGAGCC ACTGTTCACC 
ATGAGCGCCC AGCAGCCGTC CATCATCCCC TTCACGGCCC AGGCCTACGA GGAGCTGTCC 
CGGCAGTTTG ACCCTCACCC ACTGGCCATG CAGTTCATCA GCCAGTACAG CCCTGAGGAC 
ATTGTCACGG CGCAGATTGA GGGCAGCTCC GGGGCGCTGT GGCGCATCAG CCCACCCAGC 
CGCGCCCAGA TGAAGCGAGA GCTCTACAAC GGCACGGCTG ACATCACCCT GCGTTTCACC 
TGGAACTTCC AGAGGGACCT GGCCAAGGGA GGCACCGTGG AGTACGCCAA CGAGAAGCAC 
ATGCTGGCCC TGGCCCCCAA CAGCACCGCA CGGCGGCAGC TGGCCAGCCT GCTCGAGGGC 
ACCTCGGACC AGTCTGTGGT CATCCCAAAT CTCTTCCCCA AGTATATCCG TGCCCCCAAC 
GGGCCCGAAG CCAACCCTGT GAAGCAGCTG CAGCCCAACG AGGAGGCCGA CTACCTCGGC 
GTGCGCATCC AGCTGCGGAG GGAGCAGGGT GCGGGGGCCA CCGGCTTCCT TGAATGGTGG 
GTCATCGAGC TGCAGGAGTG CCGGACCGAC TGCAACCTAC TGCCCATGGT CATCTTCAGC 
GACAAAGTCA GCCCACCGAG CCTCGGCTTC CTGGCTGGCT ACGGGATCAT GGGGCTGTAT 
GTCTCCATCG TGCTGGTCAT CGGCAAGTTC GTGCGTGGAT TCTTCAGCGA GATCTCGCAC 
TCCATTATGT TCGAGGAGCT GCCGTGCGTG GACCGCATCC TCAAGCTCTG CCAGGACATC 
TTCCTGGTGC GGGAGACTCG TGAGCTGGAG CTGGAGGAGG AGTTGTACGC CAAGCTCATC 
TTCCTCTACC GCTCACCAGA GACCATGATC AAGTGGACTC GTGAGAAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007992535.1
CDS	208..7401
Translation	MEGLSGATRWWEPAVLGSGSGWRAAADRDDDERDVDASPPAGLQEAATLAPTRRSRLAARFRVTAHWLLVAAGRVLAVTLLALAGIAHPSALSSVYLLVFLAICTWWACHFPISTLGFNRLCVAVGCFGAGHVICLYCYQTPLAQALLPPAGIWARVLGLKDFVGPANCSSPHVLVLNTSLDWPMYASPGVLLLLCYATASLRKLRVHRPSGQRKEAAKGDETRELELAELDQWPQDGEAAQHVVPTAPDMEADNCIVHELTGQSPLRQRPVRPKRAEPREASPLHSLGHLIMDQSYVCALIAMMVWSITYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGMTLCCLRYVWAMDLRPELPTTLGPVSLRQLGLEHTRYPCLDLGAMLLYTLTFWLLLRQFVKEKLLKRAEAPAALMEVTVADTEPTRTQTLLQSLGELVKGIYAKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYSLWRKLLKAFWWLVVAYTMLVLIAVYTFQFQDFPAYWRNLTGFTDEQLGDLGLEQFSVSELFSSILVPGFFLLACILQLHYFHRPFMQLTDMEHVSPPRRRLPRWAHRQDAVSGTPLLQQQQQEEEEEEGPRDEGLGMATPHQATQVPEGAAKWGLVAERLLELAAGFSDVLSRVQVFLRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCVIIVCKMLYQLKVVNPQEYSSNCTEPSPNSTNLLTMEINQSLLYRGPVDPANWFGVRKGFPNLGYIQNHLQVLLLLVFEAIVYRRQEHHRRQHQLAPLPAQAVFASGTRQQLDQDLLGCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVTLHGCWLVAILTRRHRQAIARLWPNYCLFLALFLLYQYLLCLGMPPALCIDYPWRWSRAVPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQCMAGVNTDRLEPLRGEPNPVPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTALLQRDTRARLVLWDCLILYNVTVIISKNMLSLLACVFVEQMQTSFCWVIQLFSLVCTVKGYYDPKEMMGRDQDCLLPVEEAGIIWDSICFFFLLLQRRVFLSYYYLHVRADLQATALLASRGFALYNAANLKSIDFHRRTEEKSLAQLKRQMERIRAKQEKHRQGRADRGHPQDTLGPKDPGLEPGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEAAPEDPRPSAQSAFQMAYQAWVTNAQTVLRRQQQEQARQKQAGQLPTGGGPSQEVEPAEGPEEAAAGRSHMVQRVLSTAQFLWVLGQALVDGLTRWLQEFTRHHSTMSDVLRAERYVLTQELLQGGEVHRGILDQLYTSEAEATLPGPTEAPDAPSTVSSGLGAEEPLSSMTDDTGSPLSTGYHTRSGSEEVVADPGEHEAGASLHQGRMRTASELLLDRRLRIPELEEAELFAEGQGRALRLLRAVYQCVAAHSELLCYFIIILNHMVTASAGSLVLPVLVFLWAMLSIPRPSKRFWMTAIVFTEVSVVVKYLFQFGFFPWNSHVVLRRYENKPYFPPRILGLEKTDGYIKYDLVQLMALFFHRSQLLCYGLWDHEEDSPSKEHDKSGRKEQGAEEGPGALPEPQAKAGTGLEEEPGVPEATTEDHVQVEARVVPTDGTPEPQVELRPRDTRRISLRLRRRRKEGPAQRGLAAIEAEDREEEKEAPTGREKRPSRAGRRVRAAGRRLQGFCLSLAQGTYRPLRRFFHDILHTKYRAATDVYALMFLADVVDFIIIIFGFWAFGKHSAATDITSSLSDDQVPEAFLVMLLIQFSTMVVDRALYLRKTVLGKLAFQVALVLAIHLWMFFILPAVTERMFSQNVVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSSWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLVRSVVGVVNQPVDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPHPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

Target ORF information:

RefSeq Version	XM_007994344.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007994344.1

ORF Insert Sequence:

ATGGAGGGAT TGTCTGGGGC CACGCGGTGG TGGGAACCTG CAGTGCTGGG CAGTGGGTCT 
GGCTGGAGAG CCGCTGCAGA CAGGGATGAT GACGAGAGGG ATGTGGATGC CAGTCCCCCG 
GCAGGGCTGC AGGAAGCCGC AACGCTGGCC CCTACACGGA GGTCACGGCT GGCCGCCCGT 
TTCCGCGTCA CTGCCCACTG GCTGCTGGTG GCGGCTGGGC GGGTCCTGGC CGTAACACTA 
CTCGCACTGG CAGGCATCGC CCACCCCTCG GCCCTCTCCA GCGTCTACCT GCTGGTCTTC 
CTGGCCATCT GCACCTGGTG GGCCTGCCAC TTCCCCATCA GCACGCTGGG CTTCAACAGA 
CTCTGCGTCG CAGTGGGCTG CTTCGGCGCC GGCCATGTCA TCTGCCTCTA CTGCTACCAG 
ACGCCCTTGG CCCAGGCTCT GCTCCCGCCT GCCGGCATCT GGGCTAGGGT GCTGGGTCTC 
AAGGACTTTG TGGGTCCTGC GAACTGTTCC AGCCCCCATG TGCTGGTCCT CAACACCAGC 
CTGGACTGGC CCATGTATGC CAGCCCCGGC GTCCTGCTGC TGCTGTGCTA CGCCACGGCC 
TCTCTGCGCA AGCTCCGTGT GCACCGCCCC TCCGGCCAGA GGAAGGAGGC GGCAAAGGGG 
GACGAGACTC GGGAGCTGGA GCTGGCAGAG CTGGACCAGT GGCCCCAGGA TGGGGAGGCT 
GCCCAGCACG TGGTGCCCAC AGCACCCGAC ATGGAGGCTG ATAACTGCAT CGTGCACGAG 
CTGACAGGCC AGAGCCCCCT CCGGCAGCGG CCTGTGCGGC CCAAGCGGGC TGAGCCCAGG 
GAGGCATCCC CACTCCACAG CCTGGGCCAC CTCATCATGG ACCAGAGCTA TGTGTGCGCA 
CTCATCGCCA TGATGGTGTG GAGCATCACC TACCACAGCT GGCTGACCTT CGTGCTGCTG 
CTCTGGGCCT GCCTCATCTG GACGGTGCGC AGCCGCCACC AGCTGGCCAT GCTGTGCTCG 
CCCTGCATCC TGCTGTATGG GATGACGCTG TGCTGTCTAC GCTACGTGTG GGCCATGGAC 
CTGCGCCCTG AGCTGCCCAC CACTCTGGGC CCCGTCAGCC TGCGCCAGCT GGGGCTGGAG 
CACACCCGCT ACCCCTGCCT GGACCTTGGC GCCATGTTGC TCTACACCCT GACCTTCTGG 
CTCCTGCTGC GCCAGTTTGT GAAAGAGAAG CTGCTGAAGC GGGCAGAGGC TCCAGCTGCG 
CTAATGGAGG TCACTGTGGC AGACACAGAG CCCACACGGA CGCAGACGCT TCTGCAGAGC 
CTGGGGGAGC TGGTGAAGGG CATCTATGCC AAGTACTGGA TCTATGTGTG TGCCGGCATG 
TTCATCGTGG TCAGCTTCGC CGGCCGCCTC GTGGTCTACA AGATTGTCTA CATGTTCCTC 
TTCCTGCTCT GCCTCACCCT CTTCCAGGTC TACTACAGCC TGTGGCGGAA GCTCCTCAAG 
GCCTTCTGGT GGCTCGTGGT GGCTTACACC ATGCTGGTCC TCATCGCCGT CTACACCTTC 
CAGTTCCAGG ACTTCCCCGC CTACTGGCGC AACCTCACTG GCTTCACCGA TGAGCAGCTG 
GGGGACCTGG GCCTGGAGCA GTTCAGCGTG TCCGAGCTCT TCTCCAGCAT CCTGGTGCCC 
GGCTTCTTCC TGCTGGCCTG TATCCTGCAG CTGCACTACT TCCACCGGCC CTTTATGCAG 
CTCACCGACA TGGAGCATGT GTCCCCGCCC CGCAGGCGCC TCCCTCGCTG GGCTCACAGG 
CAGGATGCAG TGAGCGGGAC CCCACTGCTG CAGCAGCAGC AGCAGGAGGA GGAGGAGGAG 
GAGGGCCCCA GGGATGAGGG GCTGGGCATG GCCACTCCCC ACCAGGCCAC CCAGGTGCCT 
GAAGGCGCAG CCAAGTGGGG CCTGGTGGCC GAGCGGCTGC TGGAGCTGGC AGCTGGCTTT 
TCGGACGTCC TCTCACGGGT GCAGGTGTTC CTGCGGCGGC TGCTGGAGCT GCACGTCTTC 
AAGCTGGTGG CCCTGTACAC CGTCTGGGTG GCCCTGAAGG AGGTGTCAGT GATGAACCTG 
CTGCTGGTGG TGCTGTGGGC CTTCGCCCTG CCCTACCCAC GCTTCCGACC CATGGCCTCC 
TGCCTGTCTA CTGTGTGGAC CTGTGTCATC ATCGTGTGTA AGATGCTGTA CCAGCTCAAG 
GTCGTCAACC CTCAGGAGTA TTCCAGTAAC TGCACCGAGC CCTCCCCCAA CAGCACCAAC 
CTGCTGACCA TGGAGATCAA CCAGTCCCTG CTGTACCGGG GGCCCGTGGA TCCCGCCAAC 
TGGTTTGGGG TGCGGAAAGG GTTCCCCAAC CTGGGCTACA TCCAGAACCA CCTGCAGGTC 
CTGCTGCTGC TGGTATTCGA GGCCATCGTG TACCGGCGCC AGGAGCACCA CCGCCGGCAG 
CACCAGCTGG CCCCGCTGCC TGCCCAGGCC GTGTTCGCCA GCGGCACCCG CCAGCAGCTG 
GACCAGGATC TGCTCGGCTG CCTCAAGTAC TTCATCAACT TCTTCTTCTA CAAATTTGGG 
CTGGAGATCT GCTTCTTGAT GGCCGTGAAC GTGATTGGGC AGCGCATGAA CTTTATGGTG 
ACCCTGCACG GCTGCTGGCT GGTGGCCATC CTCACCCGCA GACACCGCCA GGCCATCGCC 
CGCCTCTGGC CCAACTACTG CCTCTTCCTG GCTCTGTTCC TGCTGTACCA GTACCTGCTG 
TGTCTGGGGA TGCCCCCGGC CCTGTGCATC GATTACCCGT GGCGCTGGAG CCGGGCTGTC 
CCCATGAACT CCGCACTCAT CAAGTGGCTG TACCTGCCTG ATTTCTTCCG GGCTCCCAAC 
TCCACCAACC TCATCAGTGA CTTCCTCCTG CTGCTGTGCG CCTCCCAGCA GTGGCAAGTG 
TTCTCAGCTG AGCGCACGGA GGAGTGGCAG TGCATGGCTG GTGTCAACAC CGACCGCCTG 
GAGCCGCTGC GGGGGGAGCC CAACCCTGTG CCCAACTTTA TCCACTGCAG GTCCTACCTC 
GACATGCTGA AGGTGGCCGT CTTCCGGTAC CTGTTCTGGC TGGTGCTGGT GGTGGTGTTT 
GTCACGGGGG CCACCCGAAT CAGCATCTTC GGGCTGGGCT ACCTGCTGGC CTGCTTCTAC 
CTGCTGCTCT TCGGCACCGC CCTGCTGCAG AGGGACACGC GGGCCCGCCT CGTGCTGTGG 
GACTGCCTCA TTCTGTACAA CGTCACCGTC ATCATCTCCA AGAACATGCT GTCGCTCCTG 
GCCTGCGTCT TCGTGGAGCA GATGCAGACC AGCTTCTGCT GGGTCATCCA GCTCTTCAGC 
CTTGTGTGTA CCGTCAAGGG CTACTATGAC CCCAAGGAGA TGATGGGCAG AGACCAGGAC 
TGCCTGCTGC CTGTGGAGGA GGCCGGCATC ATCTGGGACA GCATCTGCTT CTTCTTCCTG 
CTGCTGCAGC GCCGTGTCTT CCTCAGCTAT TACTACCTGC ACGTCAGGGC CGACCTCCAG 
GCCACTGCCC TGCTGGCCTC CAGGGGCTTT GCCCTCTACA ACGCTGCCAA CCTCAAGAGC 
ATTGACTTCC ACCGCAGGAC AGAGGAGAAG TCCCTGGCCC AGCTGAAAAG ACAGATGGAG 
CGTATCCGTG CCAAGCAGGA GAAGCACAGG CAGGGCCGGG CGGACCGCGG TCACCCCCAG 
GACACCCTGG GCCCCAAGGA CCCAGGCCTG GAGCCAGGGC CCGACAGTCC AGGGGGCTCC 
TCCCCGCCAC GGAGGCAGTG GTGGCGGCCC TGGCTGGACC ACGCCACAGT CATCCACTCC 
GGGGACTACT TCCTGTTTGA GTCTGACAGC GAGGAAGAGG AGGAGGCTGC CCCTGAAGAC 
CCTAGGCCAT CGGCCCAGAG TGCCTTCCAG ATGGCATACC AGGCATGGGT GACCAACGCC 
CAGACGGTGC TGAGGCGGCA GCAGCAGGAG CAGGCAAGGC AGAAGCAGGC AGGACAGCTG 
CCCACAGGAG GTGGCCCCAG CCAGGAGGTG GAGCCGGCAG AGGGCCCCGA GGAGGCAGCG 
GCAGGCCGGA GCCACATGGT GCAGAGGGTG CTGAGCACGG CGCAGTTCCT CTGGGTGCTG 
GGGCAGGCGC TGGTGGACGG GCTGACGCGC TGGCTGCAGG AGTTCACCCG GCACCACAGC 
ACCATGAGTG ACGTGCTGCG GGCAGAGCGC TACGTCCTCA CGCAGGAGCT CCTGCAGGGT 
GGTGAAGTGC ACCGGGGCAT ATTGGATCAG CTGTACACAA GCGAGGCTGA GGCCACACTG 
CCAGGCCCCA CCGAGGCCCC CGACGCACCA AGCACAGTGT CCAGTGGGCT GGGCGCGGAG 
GAGCCACTCA GCAGCATGAC AGACGACACG GGCAGCCCCC TGAGCACTGG CTACCACACG 
CGCAGTGGCA GCGAGGAGGT GGTCGCTGAC CCCGGGGAGC ACGAGGCTGG CGCCTCTCTG 
CACCAGGGAC GCATGCGGAC GGCCAGCGAG CTGCTGCTGG ACAGGCGCTT GCGCATCCCA 
GAGCTGGAGG AGGCAGAGCT GTTTGCGGAG GGGCAGGGCC GGGCGCTGCG GCTGCTGCGG 
GCCGTGTACC AGTGCGTGGC TGCCCACTCA GAGCTGCTCT GCTACTTCAT CATCATCCTC 
AACCACATGG TCACGGCCTC TGCGGGCTCG CTGGTGCTGC CCGTGCTCGT CTTCCTGTGG 
GCCATGCTGT CCATCCCGCG GCCCAGCAAG CGCTTCTGGA TGACGGCCAT CGTCTTCACC 
GAGGTCTCAG TGGTCGTCAA GTACCTGTTC CAGTTTGGAT TCTTCCCCTG GAACAGCCAC 
GTGGTGCTGC GGCGCTACGA GAACAAGCCC TACTTCCCGC CCCGCATCCT GGGCCTGGAG 
AAGACTGACG GCTACATCAA GTACGACCTG GTGCAGCTCA TGGCCCTCTT CTTCCACCGC 
TCCCAGTTGC TGTGCTATGG CCTCTGGGAC CACGAGGAGG ACTCACCATC CAAGGAGCAT 
GACAAGAGCG GCAGGAAGGA GCAGGGGGCC GAGGAGGGGC CAGGGGCCCT GCCGGAACCC 
CAGGCCAAGG CAGGCACAGG GCTCGAGGAG GAGCCAGGAG TGCCCGAGGC CACCACCGAG 
GACCATGTTC AGGTGGAAGC AAGGGTTGTA CCGACGGATG GGACCCCAGA ACCCCAAGTG 
GAGCTCAGGC CCCGTGACAC GAGGCGCATC AGTCTACGTT TGAGGAGGAG GAGGAAGGAG 
GGCCCAGCAC AGAGAGGACT GGCTGCCATC GAAGCTGAGG ACAGGGAGGA AGAGAAAGAG 
GCCCCCACGG GGAGAGAGAA GAGGCCAAGC CGCGCTGGAA GAAGAGTGAG GGCGGCTGGG 
CGGCGGCTGC AGGGCTTCTG TCTGTCCCTG GCCCAGGGCA CATACCGGCC GCTACGGCGC 
TTCTTCCATG ACATCCTGCA CACCAAGTAC CGCGCAGCCA CCGACGTCTA TGCCCTCATG 
TTCCTGGCCG ACGTCGTCGA CTTCATCATC ATCATTTTTG GCTTCTGGGC CTTTGGGAAA 
CACTCAGCGG CCACGGATAT CACGTCCTCC CTATCAGACG ACCAGGTACC CGAGGCCTTC 
CTGGTCATGC TGCTGATCCA GTTCAGTACC ATGGTGGTCG ACCGCGCCCT CTACCTGCGC 
AAGACTGTGC TGGGCAAGCT GGCCTTCCAG GTGGCGCTGG TGCTGGCCAT CCACCTATGG 
ATGTTCTTCA TCCTGCCTGC CGTCACTGAG AGGATGTTCA GCCAGAACGT GGTGGCCCAG 
CTGTGGTACT TTGTGAAATG CATCTACTTC GCCCTGTCCG CCTACCAGAT CCGCTGTGGC 
TACCCCACCC GCATCCTTGG CAACTTCCTC ACCAAGAAGT ACAATCACCT CAACCTCTTC 
CTTTTCCAGG GGTTCCGGCT GGTGCCGTTT CTGGTGGAGC TGCGGGCAGT GATGGACTGG 
GTGTGGACGG ACACCACGCT GTCCCTGTCC AGCTGGATGT GCGTGGAGGA CATCTATGCC 
AACATCTTCA TCATCAAATG CAGCCGAGAG ACAGAAAAGA AATATCCACA GCCCAAAGGG 
CAGAAGAAGA AGAAGATCGT CAAGTACGGC ATGGGTGGCC TCATCATCCT CTTCCTCATC 
GCCATCATCT GGTTCCCACT GCTTTTCATG TCACTGGTGC GTTCCGTGGT CGGGGTCGTC 
AACCAGCCCG TCGATGTCAC CGTCACCCTC AAGCTGGGCG GCTATGAGCC ACTGTTCACC 
ATGAGCGCCC AGCAGCCGTC CATCATCCCC TTCACGGCCC AGGCCTACGA GGAGCTGTCC 
CGGCAGTTTG ACCCTCACCC ACTGGCCATG CAGTTCATCA GCCAGTACAG CCCTGAGGAC 
ATTGTCACGG CGCAGATTGA GGGCAGCTCC GGGGCGCTGT GGCGCATCAG CCCACCCAGC 
CGCGCCCAGA TGAAGCGAGA GCTCTACAAC GGCACGGCTG ACATCACCCT GCGTTTCACC 
TGGAACTTCC AGAGGGACCT GGCCAAGGGA GGCACCGTGG AGTACGCCAA CGAGAAGCAC 
ATGCTGGCCC TGGCCCCCAA CAGCACCGCA CGGCGGCAGC TGGCCAGCCT GCTCGAGGGC 
ACCTCGGACC AGTCTGTGGT CATCCCAAAT CTCTTCCCCA AGTATATCCG TGCCCCCAAC 
GGGCCCGAAG CCAACCCTGT GAAGCAGCTG CAGCCCAACG AGGAGGCCGA CTACCTCGGC 
GTGCGCATCC AGCTGCGGAG GGAGCAGGGT GCGGGGGCCA CCGGCTTCCT TGAATGGTGG 
GTCATCGAGC TGCAGGAGTG CCGGACCGAC TGCAACCTAC TGCCCATGGT CATCTTCAGC 
GACAAAGTCA GCCCACCGAG CCTCGGCTTC CTGGCTGGCT ACGGGATCAT GGGGCTGTAT 
GTCTCCATCG TGCTGGTCAT CGGCAAGTTC GTGCGTGGAT TCTTCAGCGA GATCTCGCAC 
TCCATTATGT TCGAGGAGCT GCCGTGCGTG GACCGCATCC TCAAGCTCTG CCAGGACATC 
TTCCTGGTGC GGGAGACTCG TGAGCTGGAG CTGGAGGAGG AGTTGTACGC CAAGCTCATC 
TTCCTCTACC GCTCACCAGA GACCATGATC AAGTGGACTC GTGAGAAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PIEZO1 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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