TGME49_313870 cDNA ORF clone, Toxoplasma gondii ME49

Gene
Clones
gRNAs

Summary

Gene Symbol	TGME49_313870
Entrez Gene ID	7896062
Full Name	zinc finger, C3HC4 type (RING finger) domain-containing protein
Synonyms	TGME49_113870
Gene Type	protein-coding
Organism	Toxoplasma gondii ME49

mRNA and Protein(s)

mRNA	Protein	Name
XM_002364556.2	XP_002364597.1	zinc finger, C3HC4 type (RING finger) domain-containing protein

Pathways from BioSystems
Gene Ontology

The following TGME49_313870 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGME49_313870 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi06259	XM_002364556.2 Latest version!	Toxoplasma gondii ME49 zinc finger, C3HC4 type (RING finger) domain-containing protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGME49_313870 ( XM_002364556.2 ) cDNA ORF clone, Toxoplasma gondii ME49 -> XP_002364597.1 Toxoplasma gondii ME49 zinc finger, C3HC4 type (RING finger) domain-containing protein mRNA.

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CloneID	OTi06259
Clone ID Related Accession (Same CDS sequence)	XM_002364556.2
Accession Version	XM_002364556.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476806400000
Organism	Toxoplasma gondii ME49
Product	zinc finger, C3HC4 type (RING finger) domain-containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_031479). On Oct 20, 2016 this sequence version replaced XM_002364556.1.

ATGAAATTCG GGAAGAGCAT CCGTCGGGAG GCGGGGAAGA ACTTGCGCGT GCGATACATC 
GACTACAAGG GTTTGAAGAA AAACATCAAA CTCGCTTCTT CGCACCTCGA GAGACAGGAC 
TACCTGCAGT GCGTGGAGTC TCTGCAGGAC TTCCATGTGG GGCTTCAGCA AGAACTGAGA 
CAAGTGCGGT CGACGTACAT TCGTCAACTC GACGAGATTC AGAAGAAGAA GGAGGTCGTC 
TGTAAACTTC TGGAGAGTCT GCGGACAGCG TCTCTCGCAG AAGAAGGCCT TCCTCAGCAG 
GACCAGGCGC GCCTCACGGT CGCAGCCGAC GGCGCGCGAG GTCTCGAAGA TGAAGTTCGT 
GAGCCCCGGC GCCCGTTTCT GGTGCAGCTG CTCTCGCCGA AACTCGGCAG TGCCGTGGCG 
AAGGTCGACA CACTCGAGGC GGCGATCGAA GTCTTTCGAA AGCGTCTCGC TTCCCCGCGA 
CAGAAAGGGA GGAGCAGCAG AGAGGCACTA ACTGGGGTGT TGGGCGAGGC CTTCAGCGCC 
TGGGGTCACG AAGACAAAGG GGAATTCGGC GACGAAGACG GCTCCTTGGG AGACGAAAGC 
GGCGACAATG GAGACGGGTG CACCGAGGAC GAGGCGGAGG CGCGCGAGAC TGGCGACGAA 
CTGCGGGAGA GACGGGCGAG GAAGGCCCAG CGACGAGAAG AAGAGCTGCG GTGGAAACTC 
GAGGGAGACA GAGGCAAAGG GATACGTCTA GATCCCGAAA AGGAGAGCGG CGGAGGGAAG 
CGAGCAGATG CAGAGGGGGA ACGAACAGCA GAGAGAGACA CTGCTTCGTG TTTTCAAGGT 
GGCGACCTCG TCGCGGAAAT CGACGCAAGA GCGACAGAAG AAAACAGAGC GAGAGGGAGA 
TGCAACGCAG AAGAGAAGAA GAGCGGAGAG GGGACGTGTG AGAAAAGACA ACAGAGTCCG 
AACTGCAGTG CAAAGAAGAG AGAAGCGGAA GTCGACGGCG AGGAAAAGTC GGAGGAGACG 
CAGGCAGAGA ATGCGGCTGG AGACGCTGAT GAAGCAGAAG GCGAAGTCGA AGAACGCACT 
GAACACGAAG AAAAAGTTTT CCAGCTATGC GTGGCTCTCC AAGAAGGGAG CTTTCAAGCC 
AGACAGCTGC GTCGATTCGT CATTTGGAAC TCGGTCGCCG TTGTGAAAAT CATTAAGAAA 
AAGACGAAGC TGCTGTGTCG ATGTCCGCCG CCGAGAAAGC GGCCTCGTCC TGTGACTTCG 
TCGCTGCTCT CTCCAGGCTC AGAGCCCCAG GCCTCGCCAC CTTCGTCGCT TTCCTTGTCC 
TCACGCTTGC GCGTGGATCA ACACCCTTGC TTGGGATGCT GTCTCCTCCG TCTGGCGCAG 
CAGCACGTCG CCCTGCAGGC CTCCGGGAGG CCGTTCCAGG AGGCGGCTGC AAGGAAGAGG 
AAAGGGGAGA AGCCAGCGAA GGAGAAGACG GCCGAAAATG CTGGAGGAAC CGCAGAACAT 
GGCCAGGAGC AGCCCGAGAA GGGAGAAGAG GCAGACGCGA ACTGCACAGG ACGAGGGAAG 
CGGCGGCACG AGGAGGGACC TCACCGCGAG GTGGCAGAGA GCAACGGAGA GAAAAGCGAG 
GACTCATGTG AGGACGAGAG GGCAAGAGAT CTGGAGGCGT CGCGGCGCGG AAGCGTTGCT 
CTGCAAGGGC GAGGCCAAGA GGTGGAGGGA GCCAGGGCAG AAGGCGGGAG TCTCCTGAGT 
GGGGAGAGCT CGAAGGAGAG ATGTGACGCA GGGACTGAAA GCGACGGCGA CTCTTCGAAT 
GCGTCCGGGC GACACTCTAG TCCGGAGAGG CCTCTGAAGC GAGACAAGGT GATGCGACTC 
GACCGCGAGA GCCCCCCTGA GGCCTCTTGT CAGACTGGGG CAGAGAGCGA GAACAAAAGC 
ATCACCATCA TGGAGGCATG CTTCTCCTCG GCTTCTTCTG TGCTTCGCCG AGAGTGCTGG 
TACTCCTCCA TGGCTCTTCC TTCGCTTCTC TCCACTCTGG ACACTCTAGT AGACGAAATT 
CTTCTCGGCC TCACTGGCCG CCCTCCGAAC GAAGATCGCC ACATCTGTCC AATCTGCTTG 
GACGTCATTG TCGACCCCGT CATTCTCCGG GCCTGTTGCC ACAGATTCTG TATCACCTGC 
CTCGCGGCAG CAGCCATCAG CAGTCCAGCC AAGGGCGAAC TTCCCAAATG TCCGTCGTGT 
CGCACTTGCA TCGTCCCGGT GGACGACGAA GCCGCCGTGG CTGCCTCTCT CGGAACAGTC 
CGCGAGGACC GAGGAGGAGT CTCTCGGCTG ACTTCGCCGT CAGGTGTGCC CGAGCCTGGA 
GGGCCGGGGA CCTCCAGACC CACTGCGGAG ACAGGCGTCC CCGGAGACTC TGCGGAGTCG 
GGGACGAGTG CGGGGGTCTT TCCTCGTGAC GAAGAGGCGG TGGGGACCGG CCAGACCGGT 
GCGGAGACAG AGGAGACAGC TGGCCACGCA GCGGTGAGGG GCGCGGAGGA TCCAGGCGAC 
TCTGGTCTGT CGCGAATTCG CGACGACTCG GGAAACGAAC CGACAAACAC TGCCGCAGGA 
GGGGGGTCGG AACCCACCCG AGCAACACCG CATCTTTCTT CTTCTGTTTC TTCTTCTCTT 
TCTTCTTCAG TGTCGGGTGC TGCTGCTGCG TCGAGTTCGG CAGCTGCGGG GTCTTCGATC 
TCTTCGTTGA AGAGCAGCTC CCTCTCTGGT GGAAAACACG AGCCGCCGCT AGCAGAGGTG 
GCGCTCGAGG CTGGCGCAGG CGAAAAGAAC GAGAGCAACC GGAAGGCGGC GGAGCTGCTG 
TCGCCGCCTC GCCGTGACGA CAGCCCAAAC AGCGGCGGAC CTCCTGCCGC CGTGAGAGTT 
CAGGCTCACC AGAGTGGAGC GGAGGCCAGA GACAGCACAG ACGCGTCAGA AGACGGACGA 
GGGGAAACCT GTCAGAGCAG AGCAGAGAAA TCGCAGGGAC TCAACGAAGC CGGACCTGAA 
AGAGTCGCGG GTTTCTCTCC AGGGGGAGAA TGCGAGCGCG AAGGCAGGAG AAGACGCACG 
TGGGAAGTTG GAAGCCAAAG ACCCGAAGCT CCCAACGTCG CCACAGATGG ATCTGCTTCT 
ATAAGAAGCG ATTCGCCACA GTCTTTTCTT TCATCTTCCT CTGCGTCTCC AAAGAGAAGC 
AGGTCAGCGG CGTCGTCGCC TGATTGCTGC GTCTTCACTT CACGGGCAGA CGCCTCGTCC 
ACCTTGGACG AAGACGGCGA TCGCACAGGG CTCTTCTTAT CTTCTTTCTC TCCCGCTTCT 
GCCTCGTCGA GTGCCTCCAT GGCTTGTTCC TCTTCTTCTT CCGCAGGTTC TTCTGCTGCT 
TGTCCGTCGT CTTCTTCTTG TTCTTCTGCG AGTTCTTCTG CTGCTTGTCC CTCCTCCAGT 
TCTTCTTGTT CTTCCTCTCC TCCCGCTCTG TCGCCGTCGT TCGGGCTCTC GAACTTCTCT 
GTGCCTCCTC CGGGGTTGTG GGGAGGGCAT TTGCGATCTC CGCCGGGACT GACACATCCT 
CTGGAGGTTG AAGGAGAGAG CAAGGCTTCT TCTGCGGAGA AGAACGTCGG TTCCTGTCCT 
CGGCCCCTGG TGTCGCCGTT TTCTCCGCTT TCTTTTCCCT CGGATTCCAC CACCATGACG 
GCTCCTTCCT TCGCTCTCTC ATCCTTCCAG GCTGCACCCC TCAGTGATTC GCGGCCGCCC 
GTCTCGTGTT CCCTCCAGGG AGAGTCATTC TCTTCTTTCG CCTCCCTGAG GGATCTCCCC 
GACCGAGTCT GCAACGAGCA GCAGCCAGAA GAACCGACAC AGGCGACGAA CGCCGCCGCA 
CTCCCCACAC TCAGGTTTTC GCATTCGCAT GCACCTCCGG CGACGGCCGC GTCGATCGGA 
GAACGCGGGA CCGTGTACGC CTCCGCCTCG CTGCTGCAGG GAGAGCGCCG GGAGTCGCTG 
GCGCGCGGCC TTGGAGGACC AGTGGCCAGC AAGCAGGAGA ATGGCGAGGG GACTGGGGCG 
CGGAAGTCGG GTCCGCTAGT CGGCTTCTCT GAGAGCCGCG AGTGTGAGGA AGGAAAGAAG 
GAGATGGAGG AAGAAGTACT GCTGCACTTC CGAGATGTTC GTCAGGTCCT CGTCCAGCAC 
CAAGTACGAG AGGCACTCCA AGAGCTCCAC GAGAGGCAGC AGCGTGAGGA TCAGTTCCGA 
CTTCTGTCGC AGCTGCTGCT CTCCTCCGAC GCGACCTCCG GGCCGGCTTC GCTCGAGGAA 
ATGCTGTGCC AGAAGGTCTC CAAGAAAGAC GAAGTTTCCG CCTTCTCGCC ACACCCGCCG 
TTCTCGGTTG CCTGCTCATC TTCCGCGTCT CTCGCCTTCC CCGCCTTCTC GTCTCCGTCC 
TTTGCCTCCT CGCCCAGCCA GCGGGCGCCC GGCAGCGCGC GCCGTGGCGG ACCGGGCCCG 
CAGAACGGAG ACAGCGGCGA GGAGACAGCG GGGCGAGGGG GCGAATTGCT GAGCTCGCGG 
TTTCTTGCCC TGGCGGGCGC ACGCGAAGAG GAAGGCAGAG ACGGGGCGAG AGGCGGAGAC 
ACTCAGGCCG GCAACGTGGC GGACTCCATT GCGAGGCGGA AGCTTCAAGA GAACTCGCCT 
GCGACGAGTC TCGAGCTCCA GCAGGAGGAG CATCTGTACA ATCAACTTTT GGTGCTGCAA 
CTGTATCTTC AGCAGCAGCG TTTCCTCGCT GAGCAGAGAC AATCGATGAA GTCGTTGTCT 
TCGTTCGAGT CCCAGGCCGC GGACCTGAGT GCGGCTTCTC TTCCTCACTC TCAGGCGTTC 
CCGGCTCCTC CCGCCTCGAC GCAAACCCAC TCCGGCGTCG TCGCTTTAGT CGAGGAACTC 
AGAACCGCAG TGGCTGCGGC GACGGGCCTC GCCGGCGGCG GGGTGTCTCC TGTGTCTCCC 
TTCTCCGCGC CTGCGTTGTC TGTTTCTACT GCAGCTCCGG CAGTTGGTTT CTCCGGAGAG 
AGCGCGAAGC CGGAGAAGGC CCGAACGGCG GAGACGATCG GGACCTTCTC AGAGGAGGCG 
CGCCGGTGCG CGGGAGGCTT GCTGAGTGGA GAGCGAGAGG ACGAGAGGGC GAAGGAGAGG 
CAGCGCCTTC ACTCAGAGAG GCCGAACAAC TTCTCGTCAC CCCAGGGGCC CATGGTCTCT 
TGCAAGTCCA GCCACGAACT TGCGGGTCTG AACTTGGACG ACTCGACTCT TGCACTCGCC 
CTGCTGCTTC AAGAGTTGCA GCGGGAGCGA GAAAGAGAAG AGAAGCCCCG CGCAGAGGAG 
AGGAAGGAGC CAGGGCGGTA CTCGGCAGGG GACTATCTCT GTCGGTCGTC GTTTTGTGGG 
GAGAACGGCG TCTCTCCTTC GCCCGACGAG GCGACTGCGA CCCTCGAGGA GCGGGAGAAA 
CAAGACGCGA TGTTTGCAGA AGGCCACTCT CGGGCTAGCG GCTTGTTTCT CCTCGGCGCG 
ACTGGCCAAG TCTCCTATCC GTATTCATCT TTCTCGCCTT ACTCCCCGTC GGCCGGAAGC 
GCTGCGGCGA GACTTCATGA GTCGTCGGGA GGCGGGAGGG CAGAAAAGGC CGATCTAGGG 
TCGACCACCG GGGCCTCTGT GGGGGCGGGA GAGAGGAAGA AGGGGAGGAG AGCAGAGGCG 
TTCTCGAGGC TTCAAGATGC TGGTCGTCGC CGTCGTTCTC TGGGATCTGC AGACCGCAAA 
CAGAAGCAAG AGCCTCCATC GCCGTCCAAC TTCCCATCGC TCCCCGGTCA AGGCGGATCG 
AGCAACGGCG GGCCATCCGT CGTCGAGCGG ACGGGGGACC TCGAGGACCC GAGCGAAGGA 
GCCTCGGGGC AGCGAAAGAA GGAGAAGAAG AGCGGGTCTC GCCACGCCGT GCATGCAGAG 
ACCATTGACC GCGTCCGAGA CTGTGGAGGG TGTGGAGAGA GAGTGATGAG GGGCGAGAAA 
GGAGAAGGGT GGAGAGAGAA GAGCGTACCG TTCCTCTCCC ATGTGCCTCT GCCTCCGCCT 
CCGCCTGTCC CGCCGCCGCC ACCGCGAGAC TTGTCGTTCG ATTCGAGAGC CGTTCTTCCT 
CCCTTCGGAA GTCCGTTTGC TTCGCGTCAC ATTCCTCACC CGCCTCCTCC CCCCGCGGGT 
CCGCCGTCGC CTCCTCCTCC GCCTCCGGCT TCGCCTCCAC CTTCTCTGTC CCCAAGTGTC 
TCTTGCGTTT TCGCGGGTGT CCCTCTCCCG GCTTCGGCCT GCTCTCCAGG AAGTGCAGCG 
TTCGCGCCGC ACCCGCCTCC TCTCCCCGAA GATGGGAGAA GCTCTCGCTT TCTCGCCTCC 
TCGGTGCCGC ACCAGGCTGT CATGGCAGGG CTGCTGCCGC CGCCAGCTCA GGGGCCGTCG 
CCCCTCGCCG TCTTGTCGAC GCTGTCTAAG CAGCGAGCCG CTGTCTTAGC AGTCGCGCCG 
TCGGAGCCGA GTGGACGACC CGGATTTCTC GCTCCCAACT CCACAAAAAA CGGTGCCTTC 
TACTCTCCGC CAGGCCCCGC CTCTGCGGCC TTCCCTCAAG CGGGTCCGGC AGGTGGGGGG 
AGCGGTCGAA AAGAAGAGGC GAGGGACAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002364597.1
CDS	633..7205
Translation	MKFGKSIRREAGKNLRVRYIDYKGLKKNIKLASSHLERQDYLQCVESLQDFHVGLQQELRQVRSTYIRQLDEIQKKKEVVCKLLESLRTASLAEEGLPQQDQARLTVAADGARGLEDEVREPRRPFLVQLLSPKLGSAVAKVDTLEAAIEVFRKRLASPRQKGRSSREALTGVLGEAFSAWGHEDKGEFGDEDGSLGDESGDNGDGCTEDEAEARETGDELRERRARKAQRREEELRWKLEGDRGKGIRLDPEKESGGGKRADAEGERTAERDTASCFQGGDLVAEIDARATEENRARGRCNAEEKKSGEGTCEKRQQSPNCSAKKREAEVDGEEKSEETQAENAAGDADEAEGEVEERTEHEEKVFQLCVALQEGSFQARQLRRFVIWNSVAVVKIIKKKTKLLCRCPPPRKRPRPVTSSLLSPGSEPQASPPSSLSLSSRLRVDQHPCLGCCLLRLAQQHVALQASGRPFQEAAARKRKGEKPAKEKTAENAGGTAEHGQEQPEKGEEADANCTGRGKRRHEEGPHREVAESNGEKSEDSCEDERARDLEASRRGSVALQGRGQEVEGARAEGGSLLSGESSKERCDAGTESDGDSSNASGRHSSPERPLKRDKVMRLDRESPPEASCQTGAESENKSITIMEACFSSASSVLRRECWYSSMALPSLLSTLDTLVDEILLGLTGRPPNEDRHICPICLDVIVDPVILRACCHRFCITCLAAAAISSPAKGELPKCPSCRTCIVPVDDEAAVAASLGTVREDRGGVSRLTSPSGVPEPGGPGTSRPTAETGVPGDSAESGTSAGVFPRDEEAVGTGQTGAETEETAGHAAVRGAEDPGDSGLSRIRDDSGNEPTNTAAGGGSEPTRATPHLSSSVSSSLSSSVSGAAAASSSAAAGSSISSLKSSSLSGGKHEPPLAEVALEAGAGEKNESNRKAAELLSPPRRDDSPNSGGPPAAVRVQAHQSGAEARDSTDASEDGRGETCQSRAEKSQGLNEAGPERVAGFSPGGECEREGRRRRTWEVGSQRPEAPNVATDGSASIRSDSPQSFLSSSSASPKRSRSAASSPDCCVFTSRADASSTLDEDGDRTGLFLSSFSPASASSSASMACSSSSSAGSSAACPSSSSCSSASSSAACPSSSSSCSSSPPALSPSFGLSNFSVPPPGLWGGHLRSPPGLTHPLEVEGESKASSAEKNVGSCPRPLVSPFSPLSFPSDSTTMTAPSFALSSFQAAPLSDSRPPVSCSLQGESFSSFASLRDLPDRVCNEQQPEEPTQATNAAALPTLRFSHSHAPPATAASIGERGTVYASASLLQGERRESLARGLGGPVASKQENGEGTGARKSGPLVGFSESRECEEGKKEMEEEVLLHFRDVRQVLVQHQVREALQELHERQQREDQFRLLSQLLLSSDATSGPASLEEMLCQKVSKKDEVSAFSPHPPFSVACSSSASLAFPAFSSPSFASSPSQRAPGSARRGGPGPQNGDSGEETAGRGGELLSSRFLALAGAREEEGRDGARGGDTQAGNVADSIARRKLQENSPATSLELQQEEHLYNQLLVLQLYLQQQRFLAEQRQSMKSLSSFESQAADLSAASLPHSQAFPAPPASTQTHSGVVALVEELRTAVAAATGLAGGGVSPVSPFSAPALSVSTAAPAVGFSGESAKPEKARTAETIGTFSEEARRCAGGLLSGEREDERAKERQRLHSERPNNFSSPQGPMVSCKSSHELAGLNLDDSTLALALLLQELQREREREEKPRAEERKEPGRYSAGDYLCRSSFCGENGVSPSPDEATATLEEREKQDAMFAEGHSRASGLFLLGATGQVSYPYSSFSPYSPSAGSAAARLHESSGGGRAEKADLGSTTGASVGAGERKKGRRAEAFSRLQDAGRRRRSLGSADRKQKQEPPSPSNFPSLPGQGGSSNGGPSVVERTGDLEDPSEGASGQRKKEKKSGSRHAVHAETIDRVRDCGGCGERVMRGEKGEGWREKSVPFLSHVPLPPPPPVPPPPPRDLSFDSRAVLPPFGSPFASRHIPHPPPPPAGPPSPPPPPPASPPPSLSPSVSCVFAGVPLPASACSPGSAAFAPHPPPLPEDGRSSRFLASSVPHQAVMAGLLPPPAQGPSPLAVLSTLSKQRAAVLAVAPSEPSGRPGFLAPNSTKNGAFYSPPGPASAAFPQAGPAGGGSGRKEEARDR

Target ORF information:

RefSeq Version	XM_002364556.2
Organism	Toxoplasma gondii ME49
Definition	Toxoplasma gondii ME49 zinc finger, C3HC4 type (RING finger) domain-containing protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002364556.2

ORF Insert Sequence:

ATGAAATTCG GGAAGAGCAT CCGTCGGGAG GCGGGGAAGA ACTTGCGCGT GCGATACATC 
GACTACAAGG GTTTGAAGAA AAACATCAAA CTCGCTTCTT CGCACCTCGA GAGACAGGAC 
TACCTGCAGT GCGTGGAGTC TCTGCAGGAC TTCCATGTGG GGCTTCAGCA AGAACTGAGA 
CAAGTGCGGT CGACGTACAT TCGTCAACTC GACGAGATTC AGAAGAAGAA GGAGGTCGTC 
TGTAAACTTC TGGAGAGTCT GCGGACAGCG TCTCTCGCAG AAGAAGGCCT TCCTCAGCAG 
GACCAGGCGC GCCTCACGGT CGCAGCCGAC GGCGCGCGAG GTCTCGAAGA TGAAGTTCGT 
GAGCCCCGGC GCCCGTTTCT GGTGCAGCTG CTCTCGCCGA AACTCGGCAG TGCCGTGGCG 
AAGGTCGACA CACTCGAGGC GGCGATCGAA GTCTTTCGAA AGCGTCTCGC TTCCCCGCGA 
CAGAAAGGGA GGAGCAGCAG AGAGGCACTA ACTGGGGTGT TGGGCGAGGC CTTCAGCGCC 
TGGGGTCACG AAGACAAAGG GGAATTCGGC GACGAAGACG GCTCCTTGGG AGACGAAAGC 
GGCGACAATG GAGACGGGTG CACCGAGGAC GAGGCGGAGG CGCGCGAGAC TGGCGACGAA 
CTGCGGGAGA GACGGGCGAG GAAGGCCCAG CGACGAGAAG AAGAGCTGCG GTGGAAACTC 
GAGGGAGACA GAGGCAAAGG GATACGTCTA GATCCCGAAA AGGAGAGCGG CGGAGGGAAG 
CGAGCAGATG CAGAGGGGGA ACGAACAGCA GAGAGAGACA CTGCTTCGTG TTTTCAAGGT 
GGCGACCTCG TCGCGGAAAT CGACGCAAGA GCGACAGAAG AAAACAGAGC GAGAGGGAGA 
TGCAACGCAG AAGAGAAGAA GAGCGGAGAG GGGACGTGTG AGAAAAGACA ACAGAGTCCG 
AACTGCAGTG CAAAGAAGAG AGAAGCGGAA GTCGACGGCG AGGAAAAGTC GGAGGAGACG 
CAGGCAGAGA ATGCGGCTGG AGACGCTGAT GAAGCAGAAG GCGAAGTCGA AGAACGCACT 
GAACACGAAG AAAAAGTTTT CCAGCTATGC GTGGCTCTCC AAGAAGGGAG CTTTCAAGCC 
AGACAGCTGC GTCGATTCGT CATTTGGAAC TCGGTCGCCG TTGTGAAAAT CATTAAGAAA 
AAGACGAAGC TGCTGTGTCG ATGTCCGCCG CCGAGAAAGC GGCCTCGTCC TGTGACTTCG 
TCGCTGCTCT CTCCAGGCTC AGAGCCCCAG GCCTCGCCAC CTTCGTCGCT TTCCTTGTCC 
TCACGCTTGC GCGTGGATCA ACACCCTTGC TTGGGATGCT GTCTCCTCCG TCTGGCGCAG 
CAGCACGTCG CCCTGCAGGC CTCCGGGAGG CCGTTCCAGG AGGCGGCTGC AAGGAAGAGG 
AAAGGGGAGA AGCCAGCGAA GGAGAAGACG GCCGAAAATG CTGGAGGAAC CGCAGAACAT 
GGCCAGGAGC AGCCCGAGAA GGGAGAAGAG GCAGACGCGA ACTGCACAGG ACGAGGGAAG 
CGGCGGCACG AGGAGGGACC TCACCGCGAG GTGGCAGAGA GCAACGGAGA GAAAAGCGAG 
GACTCATGTG AGGACGAGAG GGCAAGAGAT CTGGAGGCGT CGCGGCGCGG AAGCGTTGCT 
CTGCAAGGGC GAGGCCAAGA GGTGGAGGGA GCCAGGGCAG AAGGCGGGAG TCTCCTGAGT 
GGGGAGAGCT CGAAGGAGAG ATGTGACGCA GGGACTGAAA GCGACGGCGA CTCTTCGAAT 
GCGTCCGGGC GACACTCTAG TCCGGAGAGG CCTCTGAAGC GAGACAAGGT GATGCGACTC 
GACCGCGAGA GCCCCCCTGA GGCCTCTTGT CAGACTGGGG CAGAGAGCGA GAACAAAAGC 
ATCACCATCA TGGAGGCATG CTTCTCCTCG GCTTCTTCTG TGCTTCGCCG AGAGTGCTGG 
TACTCCTCCA TGGCTCTTCC TTCGCTTCTC TCCACTCTGG ACACTCTAGT AGACGAAATT 
CTTCTCGGCC TCACTGGCCG CCCTCCGAAC GAAGATCGCC ACATCTGTCC AATCTGCTTG 
GACGTCATTG TCGACCCCGT CATTCTCCGG GCCTGTTGCC ACAGATTCTG TATCACCTGC 
CTCGCGGCAG CAGCCATCAG CAGTCCAGCC AAGGGCGAAC TTCCCAAATG TCCGTCGTGT 
CGCACTTGCA TCGTCCCGGT GGACGACGAA GCCGCCGTGG CTGCCTCTCT CGGAACAGTC 
CGCGAGGACC GAGGAGGAGT CTCTCGGCTG ACTTCGCCGT CAGGTGTGCC CGAGCCTGGA 
GGGCCGGGGA CCTCCAGACC CACTGCGGAG ACAGGCGTCC CCGGAGACTC TGCGGAGTCG 
GGGACGAGTG CGGGGGTCTT TCCTCGTGAC GAAGAGGCGG TGGGGACCGG CCAGACCGGT 
GCGGAGACAG AGGAGACAGC TGGCCACGCA GCGGTGAGGG GCGCGGAGGA TCCAGGCGAC 
TCTGGTCTGT CGCGAATTCG CGACGACTCG GGAAACGAAC CGACAAACAC TGCCGCAGGA 
GGGGGGTCGG AACCCACCCG AGCAACACCG CATCTTTCTT CTTCTGTTTC TTCTTCTCTT 
TCTTCTTCAG TGTCGGGTGC TGCTGCTGCG TCGAGTTCGG CAGCTGCGGG GTCTTCGATC 
TCTTCGTTGA AGAGCAGCTC CCTCTCTGGT GGAAAACACG AGCCGCCGCT AGCAGAGGTG 
GCGCTCGAGG CTGGCGCAGG CGAAAAGAAC GAGAGCAACC GGAAGGCGGC GGAGCTGCTG 
TCGCCGCCTC GCCGTGACGA CAGCCCAAAC AGCGGCGGAC CTCCTGCCGC CGTGAGAGTT 
CAGGCTCACC AGAGTGGAGC GGAGGCCAGA GACAGCACAG ACGCGTCAGA AGACGGACGA 
GGGGAAACCT GTCAGAGCAG AGCAGAGAAA TCGCAGGGAC TCAACGAAGC CGGACCTGAA 
AGAGTCGCGG GTTTCTCTCC AGGGGGAGAA TGCGAGCGCG AAGGCAGGAG AAGACGCACG 
TGGGAAGTTG GAAGCCAAAG ACCCGAAGCT CCCAACGTCG CCACAGATGG ATCTGCTTCT 
ATAAGAAGCG ATTCGCCACA GTCTTTTCTT TCATCTTCCT CTGCGTCTCC AAAGAGAAGC 
AGGTCAGCGG CGTCGTCGCC TGATTGCTGC GTCTTCACTT CACGGGCAGA CGCCTCGTCC 
ACCTTGGACG AAGACGGCGA TCGCACAGGG CTCTTCTTAT CTTCTTTCTC TCCCGCTTCT 
GCCTCGTCGA GTGCCTCCAT GGCTTGTTCC TCTTCTTCTT CCGCAGGTTC TTCTGCTGCT 
TGTCCGTCGT CTTCTTCTTG TTCTTCTGCG AGTTCTTCTG CTGCTTGTCC CTCCTCCAGT 
TCTTCTTGTT CTTCCTCTCC TCCCGCTCTG TCGCCGTCGT TCGGGCTCTC GAACTTCTCT 
GTGCCTCCTC CGGGGTTGTG GGGAGGGCAT TTGCGATCTC CGCCGGGACT GACACATCCT 
CTGGAGGTTG AAGGAGAGAG CAAGGCTTCT TCTGCGGAGA AGAACGTCGG TTCCTGTCCT 
CGGCCCCTGG TGTCGCCGTT TTCTCCGCTT TCTTTTCCCT CGGATTCCAC CACCATGACG 
GCTCCTTCCT TCGCTCTCTC ATCCTTCCAG GCTGCACCCC TCAGTGATTC GCGGCCGCCC 
GTCTCGTGTT CCCTCCAGGG AGAGTCATTC TCTTCTTTCG CCTCCCTGAG GGATCTCCCC 
GACCGAGTCT GCAACGAGCA GCAGCCAGAA GAACCGACAC AGGCGACGAA CGCCGCCGCA 
CTCCCCACAC TCAGGTTTTC GCATTCGCAT GCACCTCCGG CGACGGCCGC GTCGATCGGA 
GAACGCGGGA CCGTGTACGC CTCCGCCTCG CTGCTGCAGG GAGAGCGCCG GGAGTCGCTG 
GCGCGCGGCC TTGGAGGACC AGTGGCCAGC AAGCAGGAGA ATGGCGAGGG GACTGGGGCG 
CGGAAGTCGG GTCCGCTAGT CGGCTTCTCT GAGAGCCGCG AGTGTGAGGA AGGAAAGAAG 
GAGATGGAGG AAGAAGTACT GCTGCACTTC CGAGATGTTC GTCAGGTCCT CGTCCAGCAC 
CAAGTACGAG AGGCACTCCA AGAGCTCCAC GAGAGGCAGC AGCGTGAGGA TCAGTTCCGA 
CTTCTGTCGC AGCTGCTGCT CTCCTCCGAC GCGACCTCCG GGCCGGCTTC GCTCGAGGAA 
ATGCTGTGCC AGAAGGTCTC CAAGAAAGAC GAAGTTTCCG CCTTCTCGCC ACACCCGCCG 
TTCTCGGTTG CCTGCTCATC TTCCGCGTCT CTCGCCTTCC CCGCCTTCTC GTCTCCGTCC 
TTTGCCTCCT CGCCCAGCCA GCGGGCGCCC GGCAGCGCGC GCCGTGGCGG ACCGGGCCCG 
CAGAACGGAG ACAGCGGCGA GGAGACAGCG GGGCGAGGGG GCGAATTGCT GAGCTCGCGG 
TTTCTTGCCC TGGCGGGCGC ACGCGAAGAG GAAGGCAGAG ACGGGGCGAG AGGCGGAGAC 
ACTCAGGCCG GCAACGTGGC GGACTCCATT GCGAGGCGGA AGCTTCAAGA GAACTCGCCT 
GCGACGAGTC TCGAGCTCCA GCAGGAGGAG CATCTGTACA ATCAACTTTT GGTGCTGCAA 
CTGTATCTTC AGCAGCAGCG TTTCCTCGCT GAGCAGAGAC AATCGATGAA GTCGTTGTCT 
TCGTTCGAGT CCCAGGCCGC GGACCTGAGT GCGGCTTCTC TTCCTCACTC TCAGGCGTTC 
CCGGCTCCTC CCGCCTCGAC GCAAACCCAC TCCGGCGTCG TCGCTTTAGT CGAGGAACTC 
AGAACCGCAG TGGCTGCGGC GACGGGCCTC GCCGGCGGCG GGGTGTCTCC TGTGTCTCCC 
TTCTCCGCGC CTGCGTTGTC TGTTTCTACT GCAGCTCCGG CAGTTGGTTT CTCCGGAGAG 
AGCGCGAAGC CGGAGAAGGC CCGAACGGCG GAGACGATCG GGACCTTCTC AGAGGAGGCG 
CGCCGGTGCG CGGGAGGCTT GCTGAGTGGA GAGCGAGAGG ACGAGAGGGC GAAGGAGAGG 
CAGCGCCTTC ACTCAGAGAG GCCGAACAAC TTCTCGTCAC CCCAGGGGCC CATGGTCTCT 
TGCAAGTCCA GCCACGAACT TGCGGGTCTG AACTTGGACG ACTCGACTCT TGCACTCGCC 
CTGCTGCTTC AAGAGTTGCA GCGGGAGCGA GAAAGAGAAG AGAAGCCCCG CGCAGAGGAG 
AGGAAGGAGC CAGGGCGGTA CTCGGCAGGG GACTATCTCT GTCGGTCGTC GTTTTGTGGG 
GAGAACGGCG TCTCTCCTTC GCCCGACGAG GCGACTGCGA CCCTCGAGGA GCGGGAGAAA 
CAAGACGCGA TGTTTGCAGA AGGCCACTCT CGGGCTAGCG GCTTGTTTCT CCTCGGCGCG 
ACTGGCCAAG TCTCCTATCC GTATTCATCT TTCTCGCCTT ACTCCCCGTC GGCCGGAAGC 
GCTGCGGCGA GACTTCATGA GTCGTCGGGA GGCGGGAGGG CAGAAAAGGC CGATCTAGGG 
TCGACCACCG GGGCCTCTGT GGGGGCGGGA GAGAGGAAGA AGGGGAGGAG AGCAGAGGCG 
TTCTCGAGGC TTCAAGATGC TGGTCGTCGC CGTCGTTCTC TGGGATCTGC AGACCGCAAA 
CAGAAGCAAG AGCCTCCATC GCCGTCCAAC TTCCCATCGC TCCCCGGTCA AGGCGGATCG 
AGCAACGGCG GGCCATCCGT CGTCGAGCGG ACGGGGGACC TCGAGGACCC GAGCGAAGGA 
GCCTCGGGGC AGCGAAAGAA GGAGAAGAAG AGCGGGTCTC GCCACGCCGT GCATGCAGAG 
ACCATTGACC GCGTCCGAGA CTGTGGAGGG TGTGGAGAGA GAGTGATGAG GGGCGAGAAA 
GGAGAAGGGT GGAGAGAGAA GAGCGTACCG TTCCTCTCCC ATGTGCCTCT GCCTCCGCCT 
CCGCCTGTCC CGCCGCCGCC ACCGCGAGAC TTGTCGTTCG ATTCGAGAGC CGTTCTTCCT 
CCCTTCGGAA GTCCGTTTGC TTCGCGTCAC ATTCCTCACC CGCCTCCTCC CCCCGCGGGT 
CCGCCGTCGC CTCCTCCTCC GCCTCCGGCT TCGCCTCCAC CTTCTCTGTC CCCAAGTGTC 
TCTTGCGTTT TCGCGGGTGT CCCTCTCCCG GCTTCGGCCT GCTCTCCAGG AAGTGCAGCG 
TTCGCGCCGC ACCCGCCTCC TCTCCCCGAA GATGGGAGAA GCTCTCGCTT TCTCGCCTCC 
TCGGTGCCGC ACCAGGCTGT CATGGCAGGG CTGCTGCCGC CGCCAGCTCA GGGGCCGTCG 
CCCCTCGCCG TCTTGTCGAC GCTGTCTAAG CAGCGAGCCG CTGTCTTAGC AGTCGCGCCG 
TCGGAGCCGA GTGGACGACC CGGATTTCTC GCTCCCAACT CCACAAAAAA CGGTGCCTTC 
TACTCTCCGC CAGGCCCCGC CTCTGCGGCC TTCCCTCAAG CGGGTCCGGC AGGTGGGGGG 
AGCGGTCGAA AAGAAGAGGC GAGGGACAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TGME49_313870 cDNA ORF clone, Toxoplasma gondii ME49

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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