FRM1 cDNA ORF clone, Toxoplasma gondii ME49

Gene
Clones
gRNAs

Summary

Gene Symbol	FRM1
Entrez Gene ID	7894395
Full Name	formin FRM1
Synonyms	TGME49_006430
Gene Type	protein-coding
Organism	Toxoplasma gondii ME49

mRNA and Protein(s)

mRNA	Protein	Name
XM_018779357.1	XP_018637251.1	formin FRM1

Pathways from BioSystems
Gene Ontology

The following FRM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FRM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi09295	XM_018779357.1 Latest version!	Toxoplasma gondii ME49 formin FRM1 (FRM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FRM1 ( XM_018779357.1 ) cDNA ORF clone, Toxoplasma gondii ME49 -> XP_018637251.1 Toxoplasma gondii ME49 formin FRM1 (FRM1), mRNA.

Price & Availability↑

CloneID	OTi09295
Clone ID Related Accession (Same CDS sequence)	XM_018779357.1
Accession Version	XM_018779357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 15030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476806400000
Organism	Toxoplasma gondii ME49
Product	formin FRM1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_031474).

ATGTCTTGCC CGGCAAGCTG CCCGCGAGGC CGCGGGGGTT CGGCCCCGCT GCCTCCCATG 
CCTCCCTCTG TTAACGACGA TAGAAACGCC GTACTAAATG CATTACCCTC GAGAACGTCT 
TTCTCTGCTC CTCTCTGCTC CGCAACTTCT TGTTCTCTAC CTGAGAGGCC CGTATTCGCC 
AAGTTGACTG GTGGCATGCC TTTCTCTCGA ACAACGGCGC GCATCGAGTG CGCGTCTCCC 
GCGTGTCATG AATCTCTATC GTTACATCGA GTGGCCGAAT CTCAGGTTAA AGCGCAAGCT 
GCCGCCTCTG GGCCTCTGTC CAATTCGTCG TTTCTCATCT TTCTCAATTC TATCCCGACA 
GAACCCCCTC GCTGTTTCCT ACCCCCCCGC CGCGTCGCCT CGCTCTCTGC CGAGGCTGAC 
ACACCCAAGG GTTCCTTCTA TGTCTTCTCC GAGAGTTCTC GGAGTGAGTC TTCGCACAGT 
GAAGGCTGTG TGGAATCCCG CTTGTCTGGG CACACAGCAG CACGCACGAG GTGCCGGAGG 
TCGCGTACCA CTGCTTCGGC TACCGACAGC CGTCGCGATG ACCTTTTTTC GTTTGACCTA 
CCGCAAGAAT GCGGTTCTGC CCCCAGACTT CCTCTTCCGC TTCCTTCCCG TTCGTGTCCC 
GCCTCTCCGT GCCACTCTTT GCTTCCTCCG CTCTCCAATA AACTCACTCC ACCCTGTCGA 
GAGAGCCAAT CTGAAGGGAC TGATCATGGC TCGTCGTCTG CGCGTTGCTG TCAGCCCCGT 
CCAGCGTATC CGCCCCGAAT CACGGATGCG CTGCCTACCG GACAGATTTA TTCTCGTTCT 
CCATCGAGGG TGCGTGAGCG TACTCTTCGA TCAGAGCCAG CAGCACCGGT AAATTATTCA 
GATACTCAAA AGCGTTGCCC TGCCCCGCTC TTCCAATCCA TTCCCGTCCA CAGCATGCCG 
CCACCCCCGC CGCCACCCAA GGGAAAGAAG GGTCCGCTGC CGAAAAAAGC CTCCAACTCG 
CCTCTCCCGC CCAAAGGCAA ATCAACCCCA GACGAAGGGG CCAAAAAAAC GCCACCACCG 
CCGCCTGCAG CCAAAGCGGG AGTTCCTGAA GGCAAAGCGG AGATGCCTTC AGAAAAGGCG 
GAAGTTCCTC AAAAAAAGGC GGGGGTGCCT TCAGAAAAGG CGGACGTTCC TCAAAAAAAG 
GCGGGGGTGC CTAAGGAAAA GGCGGGAATT CCTCAAGAAA AGGCAGGAAT TTCTAAAGGA 
GAATCGGGGA GTCCTGAAGG AAAAACGGGT CCTCTTCCAG GAAAAGCAGG ACCTCCCAAA 
GGAAAGCTGC CGCCTGGTAA AGCGCCCCCT GCAGAAGGGT CTGGTCCTCC GGCAAAGAGC 
GATTTGAAGG CTTCACCTGC AGGCGCCCCG CCCTCTTCGC TTCCTTCCAA GAAGGCTGTC 
GCGTCGGCTG GGAAACCCCT GGGAGGCAAG ACGCCTCCAG CGCCGAGCGT GGCTCCGAAC 
CCACCTCTCG GCAGTTCCGG CCCGCAAGAG TCTGGCTCTT CGACGTACTA CAGAGACACT 
CTGCGAGCAG CTGCGGGCAC GGCGGGCGGG ATCGAGTGCT CAGCGGGGGA GGGAAGCGGG 
GAAACTGTGC CGCCCCCAAA CGAGGCGTAC GCCGCGAAAG CCGCCATGAT GGTGGAGAGT 
CTCAAACTGG CAGACTACAC GAACGTCCCA GCCGCCCACG CGCACGGAAC TGAGCCGCCT 
GCTCTGCCGT TTGCGCCGGG CGCTGACCGG AACCCAGCGA TTTTGACGAG GGCTTTGAAG 
AAGGAAATGG AGTTGCGTCG CGGGCAACCC ACGCAGACCC CCGAGGAGCG GCGGCTGATG 
CTTGCAGGCA CCTTCAGTCT GCACCAAGCG CGGCTCGGGT TCAAGTTGTC ACCGGAGCAC 
CAGCGCGTTG CCGAAGCCCT GATGCTTCTG CGGCGTTTGG AGGACTTCCG CGGCAGCGTC 
CAGGCCGGCG AAGTCCAGCG GATTCGGTAC TTGCTCAGAG TCGGAGCCAC CGAGGCGTTC 
AGCCAGCAAA GGTACTCGGA CGCCATGCTC CAGGCGCTCC ACTCCTACTT GCTCGCAAAG 
TCGTACTACA CAGCGTACCA AAGCGCGCCT CTCGAGGGCG AAATGGTTCC CGAGTTGCTT 
CTTCTCGTGA AGTGCTGCGG CCTGGCGGGC TGCCCCGACC GTGGACAGGA GTATCTGAAG 
GAGCTCCGGT ACATCGTGGA AAATACTTTG ATTGCAGAGG CCGGAGGCAG CGCGGCGGGA 
AACCGAGGAA GTCTGGTCGG CCCGATCATG CGGGTCGAGA AACACGTCCT CGCTTCAGTC 
GTCAGCAGTC TCGCTGAACT GTGCAGTCTG TACAACCAAC CGGACGACGC CAGGCTCTTC 
TACGAAAAAT ACGTGATTCT CACAAAAGAC GTGTATGGCG CAGATTCTCT GGCGGTGAGC 
GACGCACTGA ACACCGTGGC TCTGTACTTC TTTCGGACTC GACAGTACGG CCAAGCGTTG 
CCGCTGGTGG AACAAGTGTT GGCCATCCGG AAAAAGCACT TGGGTGACTT TGACGCTGCG 
GAACCCCACG CCAGAGTCGC GGACTGTTAC GCAAACATTG GCCTTCTTCA TCGACTTCTC 
GGTCACCCTA CGGAGGCGTT TCCGTGTGTG TTGATCGCTC TGGACATGCG CATGCGAATT 
TACCAGACGC GGGAGACGTC AGGCGTCCAG GACTTGGTCT TGGCTCTGGG GTGTCTGCAG 
CATCAAGCCG GCCACTTTCG GGCGGCTCTC GAGGCGTACA AAGAAGTGTG GACGTTCAGG 
AACAACACTC TGGGTGTCAC GCATCCCGAC ACGGTGAACG TCGAGTTGTT GCTGAGGCAG 
TTGGAGAAAG ACCTGGCAGC GCAGATGCAG GAAGACAAGC AGCGCTTTGT TCAGCGGGAC 
AAGGCATTGC CGTCCGCGTC GGCGTTCGAC CTCACGACTC GCGCAAGCTC ACAGATTTCC 
GTTTCCCGTC AGAGTCACCG GCCCGGGGAC TTTTTCTACG AGGAAGCCGA CGGGGCGGCG 
AGCTCGGACC GCGGTCGCAG GGGAAGTCTG GACTTTGCAG ATGCTTCCGC GACGGTGCGG 
CTGTTGAAGC CGGCAGCGGC GGCGACTCTG GAGGCGTACA GACAGCGCCA CGCTGCGTGC 
GGGTGGACGC AGCCCCTACG AGAGAAGAGC CATTTCCACT CTGTGGCTCG CGTCCGGGCG 
CTCTACGGAG AAGAGTGGCG TTCGCTCATG GTTCCCTCAG CGGCGATTCT GCCCTACGTC 
GACGTCGAGG TCACTGGCCC CGATACGCTG AAGCTGGCGG AGTGCTTTCA GTTCCAGGAA 
GCTCTCGCCG TCGACCGGCC GACCCTCCCG GTGGTGAACG TTCCCCAGAT TGAGAGGGGA 
GAGGCCGTCC TCGAGCAGGG CCATCCTCAC ATGGTGTTGA GTCCGGACGC CGCCGAGCTG 
CTCTCGCAGT GGGGCGTTGC GCTTCCCGAG GTCGACGAGA AGGGCCAGGT CATCAACCGG 
GAGGCGAAGC TCGAGAAGCA CGGGCGTCTT TTCCTGCCAC TGGTCGACCC GAGCACGAAC 
CAGCCGTTCC TGGGATTTTA CGGAGAGCCT GTGATGGTGC CGAATCCGGC GGTCTACCAC 
AGCGTGATTG CGCCTGCCAT TCTCGCCTAT GCGCGCCGGC AAGCGGCTCT GGTGGACCGA 
GCGAAGAACG CCGTCGGGCC TGCGCAGAAG CAACGCTCCG CGATGGTCCA GGGCGCGAAG 
CAAGTGGCGC GCGGCGTCGT CATGGCGAAA CTCGGGGCGA AGGGCAAGGC GGGTGCGAAG 
ACACCTGCGC AGGACGCACC CAAAGGCCTG GAAACGAAGG ACGCCCCCGC CACTGCGGCT 
CCCGCAGCAA AAGAAGGTGG CGCACCCGAG ACAGCAGAAA ACGCTCCTCC GAAAGTGGAG 
GCGGCTGCTG CCGCTCCTGA CAAAGAAGAG TCAGTTGAGA AGAAGGAAGA AGCTTCGCCG 
AGTCTGCCGC GGAAGGTGTC GGAACTTCCT GCGAAGAAGA GTCTCTTGAG GTCGAAGGCG 
AGCCAAGAGA TTCCGAAGGC GCCTCCGCTG AAGGACGACT CTTCGGCACC CGAATCAGGG 
AGTGACAGCG GCGCGAGTGA CCGGAAGCGC TCCCCGTCTC TTTCCACGAA GAAGTCTTTC 
GCGAAGAAGA CGTCTGCGGC CCTGCCGACC CCCGCACCTG AAGGTGCGAC TGCGGACGGG 
AAGCCCAAAT CGCTTCTCGG AAGCGGCGCC AAGAAATTCG ACCCTTCCGC CTACAAAGCA 
AAAGTGAAGG CGGGACCTGC ACCTGGGAAG GCCTTGTCGT TTCGTGCCAC TCCGAAGCAT 
GCAGCCGCGT TGCCGCCTTC AAATCACCTT CGCGAGCCCG CGACCCTGTC CGATCCGGAC 
GCGATTCTCG AAAGAAAACA GAAGTCCCGG ACGAAAGCGC AGACGACCGC GGCCGACTCA 
CACGAGCTCG GGCGCAACGA CATGTTAGAA CGAGAAGAAG AGGGAGACGG AGGAGGAGAA 
GAGGGACTGG CCAAGAGGAA GAAAGTGGTT TTCCAGAAGA ATGTGGAAAA AGGCAGGAAG 
GTCTCTGAGC TTCAACATGA GGCTTCGAGG TCAGCGGACG GGCGCGTAGG GAAGAACCAG 
CAGGGACCGG CCGCAGGGAG ACACGGTTTG AAAGCGAAAA AATTGAACGC TGAGACAGAA 
CGAAATTCTG AGTCAGAAGA GAAGAGTTCT TGCGGTTTCG CAGCACCGCA GTCAAGCTGT 
ACACGAAAAC GCGAGGGTTC CCCAGAAACG CGTTGCATCA ACGTCGAGTC CGTTCCCCAA 
CCCCAGGAGG GTCCAGACGG GTCGCGCGAC ACAACACTGG CGTCTCTTTC CCCTCCAGAC 
TCTTCAAGTT CTTTGAGATG TAAAAAAGCG TCTTCGAAGC TCGAGCTCTC TGTCGAATGC 
TCTGCTTCCC GCGACGAACC GTCCGAGGCG GAAGACCGCC GAGAAGAGAA CAGACGAGAG 
GAGAGAAGAG AGAAGACGAG ACGCGATTTC AAGGCGCTTC TGCTGAAGAA GCAACGGGAG 
CACATGAGCG AGACTTTGAT GCGACTGCAG AAAGAGACCG ACAGGGAGGC TTCGGAGTCT 
GCGAGTGAAT GCAAAATTTT TTTGAAAATG CAGCGCTCCC TCGAACGCAA AGACGAAGTG 
GGTCTCTTTT TTCCCCGCGC CTCTGGCAAC CAGACAACTC CTCCCGCCTT CCAACGAGCG 
TCCTGGCAGG CTGCAGCGCT CGCAGGTCAT CTCCGTTCAG GAGACAGTCT CTGGACGAGA 
GGCGAGGAGA CAGCTGAAGC AGACATTATG GGACGCGGGG ATGAGAGTGC TCTCTTGACA 
AGACGAGTTG AAGAAGAGAG GAAACGCGTT AGGGAGAGAC TGTTGGGATG CGACACTCGC 
GCTTCTCACA GGGAGACGAG TCTAGGGGCC GACAGCGGAC TCTTTGCCCT AGATCGAGAA 
GCACAAAGAC CGGCGGAAAA CAGGGAGTTT CCAGAACCCC ACTGCGTTTC TTCGTCACCT 
CGAAAAGCGA TTGATCAGGT GCCGGACTTG AACTGTAGAG CCGCCTTGGA TCCCGAGAGA 
AAAAGCTTTT CGCCGGACTC TGACCGAGAA ACGTCTATTG CGCTCTGCCG CCGTCGAGTG 
TCTCCCTCGG GCCCTGTCTC CTCTCCAGAC TCGCGATGGC GAGGGGACCG GAGGCGAAAC 
GCTTCATCGC GGCCGACTCT TGATTGGTGT CTTTCTCAGC AAGGAAACGC GACAGAGTCT 
TCGTCTCCAC ACAGAGACAC AACCTCCAGA GCGCCAGATT CCCCCCATGT CAAAGCCGCT 
TCCAAAGAGT CAAACTTCCT CTCCATGCCC TTCTTGGCTG CGGCCGCGCC CCCGCCCCCG 
GGGCTCTTTT CGCCCTGGGC GTCTCCGGTG AACTCGGACG GAAATGGGGA CACTGAGGGG 
ACACTGTGGG ACGAGGGGAT CGAGGCAGGT GGGTCCAGTT TGACGAGAAG GACGCGCTCT 
TCGGAACGCT CCCTGGAAAA GAGATCTGTT CGACAGAAAG CTCCTTCACA GGTTTCGGAG 
GCGCGCGGAA CATTGATGTC TTCGTTGAAC GCCGGACTGG AGGCGAAGAA GACGCGGGCC 
GACGAACGCA GAGACATCGG GACCGTGTTC TGCCTTTCGG ACTCGCGAGA CACAGAAGTG 
AAACCCGGAG AAGAGACACT GTTCCAGAGT GCCGCTGGCG AGTCGTGGGG TGTCGACCGG 
AGATACGTCG AGGCTGAGAG AGAGGCTGAG CAGAGGCGGA GACGAAGGAC AGGAGGCTGG 
GGGAGACACG AGGGAACTGC GAATCCGGGA GGTAGAGGAA GGACTGGATC TCGAGGAGAA 
GCGAGCACCT GGAGGAGGGA GAGCACGAGC TCGGCGACCG AGGCTGAGAT AAGGAGAGAA 
CGCGTTGAGC AGTCGCGTCC CGACAGTGGA AGAAGGCGTG ACACGAGAGA AAGAAAACGA 
TCTGTTGCGC AGGGCGCTGT GAATCGGGTT TCGAGAGCGA CGGGGCGAAC TGAAGGAGTC 
GCCGTCCTCG GTCAGGAGAG GCGGAGATGC GTGGACGCAC GCCGAAGAAA CGAAGCGGAA 
GACAGAGAAG GCGAGGATCT TGGAGAAGCG GAGCTCTCTG TTTCCTGCTT TGATTCTGAC 
GGAGACCAGG AGAAGGACTT TCGACGTGAA ACGTCGAGCG AAGAAGACAA CGAAGAAGAC 
GGTGAGGAAG CAGAGTTGTT TTCAACAAAC GAGGTAGAGA CCGACACGCT GCGCTCTGGG 
AGAAGGAAAC AACATTCGGT CTCCTCTCGA AGTGCTTCTC CTTCGCCATG GCGCGTTGGG 
GCAGACAGAA CTCGGATGAA AACGAACTCG CAAGCGTCCG CTGCGCTTTC GGGGATGCGT 
ACATCTCGTT TTCTTCAACC TCCCGCCTTC GATTCTTCTG CGTCGCGGAG CTCTTCTGCG 
TCGCCTTCTC GACGTCGCCG ATTGGAGACT TTGGAGACTT TGGATCTGCA GAAACGCACG 
AGAAAACAGC GAGTGCGAGT AGGAAGCCGA GAGTCCCGAG AAGGAAACGC GCAAAGGAGT 
CCCGGAAACC GACGGAGAGA CAAAAAAGGA CAGACTGAAG GGAGGAGAGG AGACTGGGGA 
AGAGCCTACT TGGAGAGGTT GGTGGTCGGA CAGGAAACAG AGACCGATTC AGAGATCGCT 
ACTCTGGATG AGGAATTGGA TAGAACGGAG TTACCTCGAG TCGTCTCTCC TCAGACCTTC 
CACGAAGCCC CAAATCCGTT TTCTGACCAA GACACAGAGA CAGATTGCTT GTTCTCCACT 
CCTCGACTTC CTGTTTCAAA TGCGGATCCG CAAGCCTCAA AAACGCACTC TGAGGCCGAT 
GAATATGTTG TTTCGTTTGC TTCTCAATCG TCTGCCGCTC GTTCTTCCTC CTGTTATCCT 
TCTCGTTCTC CCTCTTGTTC TGCTTTGTCT CTCTCTTTTG CATGCTCGGA CTCCACTGTA 
GGCGCCGAGA GCGCCACGGG GAGAGAGCGC ACTGTTCATT TGCTTCGCCC GACTTCCTCC 
AGTGTCTCTT CTATTTCGCC CACCTCTTCC GACTGCGGTA GACTCGACAG CGAACTCGAC 
GCACTTCCAG GACTCGGAAA ATGCACTTTG TTTTTCTCGG CTCCTGAGGC GGTGCATGCG 
AGGCCCCTGG CGTCGCCGAG CTTCCGGCAG CGGCTCTTCA GCCGCAGAGA CAGTGGGGCA 
GAGAGGGCCA GCTGCGGAGA CGCGTTCGAT AGTTGGAGCC CAACTAGCGC CGGTGCGGGT 
TCGCGGGTTG TGGAGAGCTG CGGCAGCAAC GTGTCGAGCC CGTCCAGGGG GAGGCTGCGG 
AGTCGCGTGA GCCTTGGCGG ATTGAAGGCC GCTTTTTCGA GGCATTCAGA AAATTCCTCG 
AACGAAAGAC TCTGCTCTCC AATGCACCAA GAAACCTCGA GGAAGGGTCC TCTCGCCGTG 
GCGAGGCGTC TCTCTGGGGT GGCCTCGCGG CTGGTGCATT CGAGGTCGAG CCTTCAAGAA 
CTCGGGGAAG CCGAAGACGA TTCGAGAAAG AGATCCAGCG AGGGAGATTC TCAGGAGATT 
CCCAGGGATT CCGTTGGCGT GACCGCACTC ACTGGAGACA GCGAGACTCC GAACGGCTGG 
GCAGGTGTGG GGGGGCTGGA AGTATCGGCG GGGGAAGACA GCAGAGAGCT CAGAGAATGC 
GAAGAAAAGG AACGAGAGAG AACGCCGCTC GGAGGGTCGC TCCGGAGATT CAGCTTCTCC 
AGCGACCTGA GAAAAGATCC GAGACGCCCA CGACGGACGC TGTCAGATGC AACGGCGACG 
TGCGAAGGGG ATGGGAAGAT GCGCCGCCTT GTCGCGCTTC TTCGCCGGAA AAATAGACGC 
GTAAAAAAAG AAGGAGAACA AGAAAGCGAG AGAGAACGGA GAGACGAAGG AGAACAAGAA 
AGCGAGAGGG AACGGAGGGA CGAAGGAGAA CACGAAGGAG AACAAGAAAG CGAGAGAGAA 
CGAAGAGACG AAAGAGAACA AGGAGGAGAA CAAGAAAGCG AGAATGAACA GGAAGCGGAA 
CATGAAAGCG AGAGAGAACG AAGAGACGAA AGAGAACAAG GAGGAGAACA AGAAAGCGAG 
AGAGAACGAA GAGACGAAAG AGAACAAGGA GGAGAACAAG AAAGCGAGAG AGAACGAAGA 
GACGAAAGAG AACAAGGAGG AGAACAAGAA AGCGAGAATG AACAGGAAGC GGAACAAGAG 
AACGAGAGAG AACAAGCGGT AGGAGAACAA GGGCAGGAGA GAGAAGGAGA GGGAGAGCAA 
AGAAGCGAAT GCGAACAAGG AGGAAGCGTG ATAAATGTTT GGAGACAGGC AGAGAAGGAA 
GAATCCTCAG GAAAGCAACT GAGTAAAGGT GAAGAGTCGA GAGAAGGAGA GAAGGGAGGC 
GTGAGAGCAG CCGACAACCA GGCGTCGGGT GCGACGCAGG GCGAAGAACA AGAGCAGAGT 
GGCTCGTCTC GTTTCTCGCA GCTCCTCGAA AAGTGGGCGA GAATGTCCGG CGGCGCGACA 
CCGAAACAAC GGATCGAGAT GATTTCAGAC AAATCCGAAC CCGGTGGAAG ATGGCGGAAG 
GACATGGGGA TTCTGAAAGA CAGTGAAGAG GAGAGTTTGA TAGGAAAGGA AGGGATTTCA 
CAGCGGCTGA CGGATGCTGA CACTGGAACT GAGAAGACGA GAGAAGCCCA GCGAGAGGAT 
GCCTGGGTCG TGACAGAAAG TGCCAGAGAC AGACGGCGAG CTTTGGGTAC GACAGTGTCT 
CTGGAGGAGG TCTCCTTGTG TGCTGTGGGG AGCTCAGAGA GTCATCTTGA AAAGCTCGGG 
AGCGGAGGTG ACGAGAACGA CTGCGGCAGA GACGCGAGTG AGGAGACCAA GCAAGGGCAT 
TGTTGCGAAG ACAGAAGAAG TCTCAAAAGA GAAAAAGAGG TGTCGGATTG GGGTGTACAG 
ACATCCCATG AGAGAGAGAA GCAGGTGTCT GCACGTGGTG TAGAGACACC AGAGACAAAA 
GAGGAATTGC CCGCTTCTCC TCTCTCTGGC GACTCTTCGT CTTCGCCTTT GGCCGGCAGT 
GCATCCAACG TCTTCCCCGC GCGCAAGCGC TTGTTTTCTT CCCGTCGAAT GCTGCGACTT 
CCGATTCCCT CGAGGAAACT CAGGAAAGCA GACAAGCCTG GAAGTCCCTC AGACGCGCAG 
AACTCTGCGG TGTCTCCCTC GCCCTCGTTT CAGCTCTCTC GGTCAGCGGA GGCCGCGACA 
GGCGGACCCC AAAACGTTGA CGAAAGAGAA GTGGCGGTGC TCTCGGGGGC GTTGGAGGTG 
CAGACCGAAG GTTGGGGTGT ACAGACAGCT CCGGAGACGC CTGGGAATTC GACACACTGG 
GCGAGTCCAA GTTTTCATTC TCTTTTTTCT CGTCGGTTTT CGCTCCGTGA TCGACTGAGG 
CGAAGAAGCT CCGCCAGCAG CATGACCGTG TTGGACATGG AGGAGAACAA GAGTCTGGAC 
GCACTTTCAG TAGATATGCC TGCAACGGAG CCCCAGCGAC CACTGGCGAG CGTTTCCGAC 
TCGCTGGTTT TTTCGGAAGG CTTGTCTTTC TCTTCCTGTG TAGACCCGGT TTCGGACTTC 
GGTGACGACA ACGAGAAGGA TGGAGTTACG CAGGAAACCC GTTCCTCTTG TTCTGAAGTG 
CCATGTGCAG GCGCGAGAGC AGGTGGGCGA GAGTCCCCGA CAAGCAGGAG ACATTCCTTC 
GTGTTTCGGA CGACCCAGCA AAAATACCGG ACAGAAGGCA GTGCAGATAA CAGAGGAAGG 
AGAAGTTTGA AGGTCCTGCG GCGTTCGAGT TTCTCGCGGG CGTTCCGGGG CTTGGTCAAA 
AGTCCTGAGA GACAAATCCT GCGTGACAGA GATCGCGAAG AGGGGAAGGC GCAGGACACC 
GCGAGGAGAT CTGAGAGCGA GAGGGAGGGT GAAAAGGACG ATGAAGAAAA GCAGACAGGG 
AAGAGGGAGG GCAGGAAGTT GCGCCACCTT TCCGCTTCGT TTCTGAGAAT TCCAAGGCCA 
GGGAAACATG AAACCAACAA GGAATCTGTT TGCAAACGCC GCGGGGCTTC CCTTCCCCCT 
TTACCGCCTG AGGCCTCGAG CCCCCACGGC GCCTCACGGC TGCGGGCTGC GGCGGCGAGT 
TTCTCGCGTC TGAGGCGACG CAGTCAATCA AGCGTTAACC GGGGGAAAAA AGAGGCGATA 
GAGATAGAAA AAATGGAAGA GGAAACGAAG GCACAGAATG AAATAAAACA CGAGAGAAAG 
AGTGAACTGA AACAGGAAGA AGTGGATGAA ATGAAACAGG AAAACGTGGA TGAAATAAAA 
CAAGAGAGAA AGGGTGAAGT CAAACAGGAA GAAGTGGATG AAATGAAACA GGAAAACGTG 
GATGAAATAA AACAAGAGAG AAAGAGTGAA CTGAAACAGG AAGAAGTGGA TGAAATGAAA 
CAGGAAAACG TGGATGAAAT AAAACAAGAG AGAAAGAGTG AACTGAAACA GGAAGAAGTG 
GATGAAATGA AACAGGAAAA CGTGGATGAA ATAAAACAAG AGACGCCGGA GACAGTGCCT 
CTCTGGGAAC CGAGCATCAC TTCCCATCTC AGTGACTCTT GCGGCGAAGA TGTCGCCATT 
TTTGCCGTTG AGGCATGCGT GAGAGCCGCA AGAACCTGGC AGAAAGAGGA TGAAACTCTG 
GGCGCTTCCG TCGCAGCGGG GAGAGGAGAA GACGAAGATG AATGCGTGGA TCGAGGAGAC 
GCGCTCACAG GGCAGGACGA CAACGCAGAG GAGACAAGCC AACGATGTAG CTGTTCCGAG 
GACAGTCCGG AGGGAGTCGC TGAACGAGAG TGGATGGGCG GAAGCAGTGA CGGCGCAGAA 
ATCCACTTGT TGCCTGAAGC AGACACCTCA GATGAAGAGG ATGAAGGAGA AGAGGAGGAA 
GAAAGAGAAG AAGAGAAAGA GGAAGAAGGA GAAGGGGAAG AAGGAGAAGA GGAAGAGGAA 
GAAGGAGAAG AGGAAGAAGA GGAAGAGGAA GAAGAGGAAG AAGGAGAAGA AGAGGACATC 
CACGAGCTGG GTGAGAAAGA ATATAGTTCA GCCGAAGAAG AAACGCGTAT TGGAGAAGCA 
GAATTGAGGC ATGGAGTCGC AATTCTTAAT CCATTTGCGT CTTGCGGGGC GGAGGCGTAT 
GGTGAGGAAA GGCCTAAGAC AGGGACGACG GGGAGTGAAG CCGATGACGA CAGCTGGGCC 
GCAAGTGACT GGAGTGTCGG CGCGAGCAGC AGCGACGAAG AGTCCGAGAG ATCTTGGCAA 
GAGGGACCTT CTTCTGAAGA AGACTGTGAC GAAACAGAAG CTGAGAAGAA CGCAGACAGT 
GAGGTGGAGG AGCTTTATGC AGAAAAGCTT TGCAGAAAGA AAGTGCATGC AGAGAACGAT 
GTCGAAGAGT GCAAGACAGA GGTTAGTAAG GCAGAGGAGG GTTTCCAGAA TTCAATGGCA 
TGTCTGGCAG AGGTGCTTCG GCTTACAGGA GTGCCGTATA GTGAAAGCGA TTTGTCTGTC 
GAGATTGCAG AGTTGAATCG ACAATCGACG CTGCTGATGG TGACTCCGGA TACACAGCGG 
CTTTCCCGTG AGCTTCCGAA GTCTATTGCG TCGCCGCGAA CGTCGGCAGT GCCGCTGGAC 
AAACAGTGCG ATGGAAAAAC GAACCTCACA CACCTTCCCA AGAGCGCACC CTTCCGGAAA 
GCGATGGAAC TCACTCGTGT CGCCTATCGG TCAGGCAAAA CGCCGGCTGC TTGTTCAGTC 
CTCCGGTCTT CGTCTTCTTC AAATCTCGCT TCTTCGGTCT CTTCAAGCCG TTCTTCTTCC 
AAACTCGTAT CTTCTTCTAG ACTGTATGCC ACCCCACGAG AGCCTCGCTC TGAGCCGCGT 
GAGGCTTCTG GTGGGTTCTC GGTGTCGCCG ATTGCGTGCG ACTCTTCTCC GTTTGCTCCA 
CCTGCGGCTC CGCTTGGCGC GTCGGAGGGT GGACGCGAAA GTCTTTCGTG CAGCGACTCT 
TCATCCGTCT TGGTTGGAGA TCGTCCCCCG CGTTTCTCGA CCCAGCAGCT CCACGCGCTC 
CCGCCGAAGG GACCGAAGGG CGGGCCGCCA AAGAAAGGCA AGGCGACGGG CTGGAAGCCT 
CCTCCGCCTC TGCCGCCAGC TGCAGAGGAC GGGACAGACG TGAAGGAAGG ACCGACAGTT 
TTGAAGCAAG GCATTTTCAA GAAAGCCGAG CAGGCGGTGA AGAAGGAGAT CCCGAAGAAG 
GAAGAGGGGT CTCCGAAGGA GGCGGCGAAG GTCGTGGAAA AGAAGGTGCT AGAGAAGAAG 
GCGAGTGTGT CCGCAGCCAA GGGCGAGACG GAGACGAAAG TCGCTGTAAA GAGCGTAGCG 
AAACCGCCGC CTCTGAAGGC TGCGAAGAGC GGCGAGGCAA AGACAGCAGA ACCTGTGAAG 
GAAGAAGCAA AAGTGGAGAA GGCCCCGAAG GTCAAGGGCG ACGGCTTTAG CCGCATGAAG 
CTCCCCTCGG GGAAGCCTGA AGAGGCGCCG AAAAAGACTC CCAAGATGAA GATCATGGTC 
GGAACGACGG GTCAGGCGAA GGTCGTCGAG AAGAAAGACT CGATGGACGA ACTGGATGCT 
TTCACGGGGC CTTGCTACAT CCCCCCGCAC ATGGACGTCC CCCCGGTCGC TCCCGAGGTA 
CTCGCAAAGA TCACCAAGTT GAAGCCTGGG GTAGAGAAGG ACCCGCAGCT GGGGGAGCTT 
CCGGTGGTCA TTCTGATGGA CGACAACAAC ACAGAGCTGG CGAGGCTTCC GTGGCAGGTG 
GACATCTCCT CGTTCTCGCT CGCACAAAGT TCGATGTCCA TCTTGGTGGC TCGGAGACTA 
GGTCTCTTGG CGCGACGCCT CGACGAAGAC AAGAAGGACG AGGACACCCG CGACTGGCTG 
ACGGGCGAGG GCCTGAAGAA GTTCGGCCTG TCGATGCAGA AGGAGAAGCC CACCTTTGGA 
GACACTAGTC TCCTCCTGGG CATGGGTCTC TCGGCGCTGC CTCAGGCCCT CGCACAGAAG 
AAGCCGGAGG CACCCAAGAA GGAGGAGGCG AAGCCAAAGC CAGAGGCGAA GAAGGAGGCG 
CCAAAGAAGG GCCCTCCCGG CGGAAAGGGC GGGAAAGGCC CTCCTGGCAA AGGGCCTCCA 
AAGGGCAAGG GCAAGGGCCC GAAGAAGAAG GGCGAAGGCG GTTTGAAGGT CGGCAAGAAG 
GACGAGGGGA AGACCAAGCG GTTCTTCTGG GACCCGATCT TCGAGGACGA AATCCCCGGG 
ACTATCTTCA TGAAGAAGCC GAACATGGTT CTAAAGCTGC AAGACGTAGA AGAGACCTTC 
GCGAAAGTCG TCGCCAAGAA AAAGACCGAG TCAAAGAAAC CCAAGATCAT CCAACTACTC 
CCAGACTCCA AACGCGCATA CAACATGAGC ATCGCCTTGT CCAAGTTCAA CAACTACTCG 
TACCAACAGC TCCGGGAGGC CATCATCGAC CTTGACCCTA AGATCCTAAC AATCGAAGCG 
ACGGAGACTC TCCTGAACTT CGTGCCCACA GGTGAAGAAA ACCAGGTCGT CAAAGAGTAC 
ATTAACTCTG GTGGAGACTT GAAACTCGTC GACAAACCGG AGCAATTCGT CGCAGCAATG 
CTCGGAGTCC CTCTGATGAA ACAGCGCCTT GAGGCCCACG CTTTCGCCTT GAACTTCCGC 
GAGGCGTACA GCGACGCGTA CACTCCACTG GAAAACATGG CAGATGCATG CGACGCCATC 
GACGATTCCC AGAACCTCAA AATTGTTCTT TTCGCCATCC TCGAGTTGGG TAACGCACTC 
AATGAGGGCG ATCCGCAGCG AGGAGGTGCA GCTGGTTTCA AACCGACAAC GCTTGCCAAG 
CTGCAAGAAA TTCGGACAAC AACGAAACCC GTCAGAACAA TGCTGCAGTA CATTTGCGAT 
ATCATTTGGG AGCAACAGCC GACGGCGCTG AACATCTATG AAGATTTGAA GATCTGCGAC 
AAGGCGCAAC GCGTTGACAT GCAGGGCATT GAGGGCAGAA TTTCCAATCT TAAGGCGGGT 
TTGACGAAGG TGAAGAACAC CCTGGAGGCA GCAAAGAAGG GCAACGAGTC CACTGGCGTC 
ATGGGAGATC GCGATCCTCT TCGGAACATT ATGGACGAAT TTCTTATAGA GGCGGAGCCG 
AAGATCAAGC AGCTTGACGA CTTCCTTGTT CAGGTTCAGA ATCAATTTCT GCAGACGTGT 
CATTACTCTG GTTACCCTGA CAAAGATGTC AAGAAGATTA AGCCAGATGA GCTTTTCAAA 
CAGGTTGCAG GCTTCGCAAG GCAGGTGGAT GCCATTCGCA AGCAGAAGCA AGAGATTGCA 
GATCGCGAAT TGAAACGCAA GGAGGCCGAG GCAAAGAGAG CAGGCAAAGG ACTCAAGTCG 
ACTGCAGTCA AGAAAGTCGG GATGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018637251.1
CDS	1854..16883
Translation	MSCPASCPRGRGGSAPLPPMPPSVNDDRNAVLNALPSRTSFSAPLCSATSCSLPERPVFAKLTGGMPFSRTTARIECASPACHESLSLHRVAESQVKAQAAASGPLSNSSFLIFLNSIPTEPPRCFLPPRRVASLSAEADTPKGSFYVFSESSRSESSHSEGCVESRLSGHTAARTRCRRSRTTASATDSRRDDLFSFDLPQECGSAPRLPLPLPSRSCPASPCHSLLPPLSNKLTPPCRESQSEGTDHGSSSARCCQPRPAYPPRITDALPTGQIYSRSPSRVRERTLRSEPAAPVNYSDTQKRCPAPLFQSIPVHSMPPPPPPPKGKKGPLPKKASNSPLPPKGKSTPDEGAKKTPPPPPAAKAGVPEGKAEMPSEKAEVPQKKAGVPSEKADVPQKKAGVPKEKAGIPQEKAGISKGESGSPEGKTGPLPGKAGPPKGKLPPGKAPPAEGSGPPAKSDLKASPAGAPPSSLPSKKAVASAGKPLGGKTPPAPSVAPNPPLGSSGPQESGSSTYYRDTLRAAAGTAGGIECSAGEGSGETVPPPNEAYAAKAAMMVESLKLADYTNVPAAHAHGTEPPALPFAPGADRNPAILTRALKKEMELRRGQPTQTPEERRLMLAGTFSLHQARLGFKLSPEHQRVAEALMLLRRLEDFRGSVQAGEVQRIRYLLRVGATEAFSQQRYSDAMLQALHSYLLAKSYYTAYQSAPLEGEMVPELLLLVKCCGLAGCPDRGQEYLKELRYIVENTLIAEAGGSAAGNRGSLVGPIMRVEKHVLASVVSSLAELCSLYNQPDDARLFYEKYVILTKDVYGADSLAVSDALNTVALYFFRTRQYGQALPLVEQVLAIRKKHLGDFDAAEPHARVADCYANIGLLHRLLGHPTEAFPCVLIALDMRMRIYQTRETSGVQDLVLALGCLQHQAGHFRAALEAYKEVWTFRNNTLGVTHPDTVNVELLLRQLEKDLAAQMQEDKQRFVQRDKALPSASAFDLTTRASSQISVSRQSHRPGDFFYEEADGAASSDRGRRGSLDFADASATVRLLKPAAAATLEAYRQRHAACGWTQPLREKSHFHSVARVRALYGEEWRSLMVPSAAILPYVDVEVTGPDTLKLAECFQFQEALAVDRPTLPVVNVPQIERGEAVLEQGHPHMVLSPDAAELLSQWGVALPEVDEKGQVINREAKLEKHGRLFLPLVDPSTNQPFLGFYGEPVMVPNPAVYHSVIAPAILAYARRQAALVDRAKNAVGPAQKQRSAMVQGAKQVARGVVMAKLGAKGKAGAKTPAQDAPKGLETKDAPATAAPAAKEGGAPETAENAPPKVEAAAAAPDKEESVEKKEEASPSLPRKVSELPAKKSLLRSKASQEIPKAPPLKDDSSAPESGSDSGASDRKRSPSLSTKKSFAKKTSAALPTPAPEGATADGKPKSLLGSGAKKFDPSAYKAKVKAGPAPGKALSFRATPKHAAALPPSNHLREPATLSDPDAILERKQKSRTKAQTTAADSHELGRNDMLEREEEGDGGGEEGLAKRKKVVFQKNVEKGRKVSELQHEASRSADGRVGKNQQGPAAGRHGLKAKKLNAETERNSESEEKSSCGFAAPQSSCTRKREGSPETRCINVESVPQPQEGPDGSRDTTLASLSPPDSSSSLRCKKASSKLELSVECSASRDEPSEAEDRREENRREERREKTRRDFKALLLKKQREHMSETLMRLQKETDREASESASECKIFLKMQRSLERKDEVGLFFPRASGNQTTPPAFQRASWQAAALAGHLRSGDSLWTRGEETAEADIMGRGDESALLTRRVEEERKRVRERLLGCDTRASHRETSLGADSGLFALDREAQRPAENREFPEPHCVSSSPRKAIDQVPDLNCRAALDPERKSFSPDSDRETSIALCRRRVSPSGPVSSPDSRWRGDRRRNASSRPTLDWCLSQQGNATESSSPHRDTTSRAPDSPHVKAASKESNFLSMPFLAAAAPPPPGLFSPWASPVNSDGNGDTEGTLWDEGIEAGGSSLTRRTRSSERSLEKRSVRQKAPSQVSEARGTLMSSLNAGLEAKKTRADERRDIGTVFCLSDSRDTEVKPGEETLFQSAAGESWGVDRRYVEAEREAEQRRRRRTGGWGRHEGTANPGGRGRTGSRGEASTWRRESTSSATEAEIRRERVEQSRPDSGRRRDTRERKRSVAQGAVNRVSRATGRTEGVAVLGQERRRCVDARRRNEAEDREGEDLGEAELSVSCFDSDGDQEKDFRRETSSEEDNEEDGEEAELFSTNEVETDTLRSGRRKQHSVSSRSASPSPWRVGADRTRMKTNSQASAALSGMRTSRFLQPPAFDSSASRSSSASPSRRRRLETLETLDLQKRTRKQRVRVGSRESREGNAQRSPGNRRRDKKGQTEGRRGDWGRAYLERLVVGQETETDSEIATLDEELDRTELPRVVSPQTFHEAPNPFSDQDTETDCLFSTPRLPVSNADPQASKTHSEADEYVVSFASQSSAARSSSCYPSRSPSCSALSLSFACSDSTVGAESATGRERTVHLLRPTSSSVSSISPTSSDCGRLDSELDALPGLGKCTLFFSAPEAVHARPLASPSFRQRLFSRRDSGAERASCGDAFDSWSPTSAGAGSRVVESCGSNVSSPSRGRLRSRVSLGGLKAAFSRHSENSSNERLCSPMHQETSRKGPLAVARRLSGVASRLVHSRSSLQELGEAEDDSRKRSSEGDSQEIPRDSVGVTALTGDSETPNGWAGVGGLEVSAGEDSRELRECEEKERERTPLGGSLRRFSFSSDLRKDPRRPRRTLSDATATCEGDGKMRRLVALLRRKNRRVKKEGEQESERERRDEGEQESERERRDEGEHEGEQESERERRDEREQGGEQESENEQEAEHESERERRDEREQGGEQESERERRDEREQGGEQESERERRDEREQGGEQESENEQEAEQENEREQAVGEQGQEREGEGEQRSECEQGGSVINVWRQAEKEESSGKQLSKGEESREGEKGGVRAADNQASGATQGEEQEQSGSSRFSQLLEKWARMSGGATPKQRIEMISDKSEPGGRWRKDMGILKDSEEESLIGKEGISQRLTDADTGTEKTREAQREDAWVVTESARDRRRALGTTVSLEEVSLCAVGSSESHLEKLGSGGDENDCGRDASEETKQGHCCEDRRSLKREKEVSDWGVQTSHEREKQVSARGVETPETKEELPASPLSGDSSSSPLAGSASNVFPARKRLFSSRRMLRLPIPSRKLRKADKPGSPSDAQNSAVSPSPSFQLSRSAEAATGGPQNVDEREVAVLSGALEVQTEGWGVQTAPETPGNSTHWASPSFHSLFSRRFSLRDRLRRRSSASSMTVLDMEENKSLDALSVDMPATEPQRPLASVSDSLVFSEGLSFSSCVDPVSDFGDDNEKDGVTQETRSSCSEVPCAGARAGGRESPTSRRHSFVFRTTQQKYRTEGSADNRGRRSLKVLRRSSFSRAFRGLVKSPERQILRDRDREEGKAQDTARRSESEREGEKDDEEKQTGKREGRKLRHLSASFLRIPRPGKHETNKESVCKRRGASLPPLPPEASSPHGASRLRAAAASFSRLRRRSQSSVNRGKKEAIEIEKMEEETKAQNEIKHERKSELKQEEVDEMKQENVDEIKQERKGEVKQEEVDEMKQENVDEIKQERKSELKQEEVDEMKQENVDEIKQERKSELKQEEVDEMKQENVDEIKQETPETVPLWEPSITSHLSDSCGEDVAIFAVEACVRAARTWQKEDETLGASVAAGRGEDEDECVDRGDALTGQDDNAEETSQRCSCSEDSPEGVAEREWMGGSSDGAEIHLLPEADTSDEEDEGEEEEEREEEKEEEGEGEEGEEEEEEGEEEEEEEEEEEEGEEEDIHELGEKEYSSAEEETRIGEAELRHGVAILNPFASCGAEAYGEERPKTGTTGSEADDDSWAASDWSVGASSSDEESERSWQEGPSSEEDCDETEAEKNADSEVEELYAEKLCRKKVHAENDVEECKTEVSKAEEGFQNSMACLAEVLRLTGVPYSESDLSVEIAELNRQSTLLMVTPDTQRLSRELPKSIASPRTSAVPLDKQCDGKTNLTHLPKSAPFRKAMELTRVAYRSGKTPAACSVLRSSSSSNLASSVSSSRSSSKLVSSSRLYATPREPRSEPREASGGFSVSPIACDSSPFAPPAAPLGASEGGRESLSCSDSSSVLVGDRPPRFSTQQLHALPPKGPKGGPPKKGKATGWKPPPPLPPAAEDGTDVKEGPTVLKQGIFKKAEQAVKKEIPKKEEGSPKEAAKVVEKKVLEKKASVSAAKGETETKVAVKSVAKPPPLKAAKSGEAKTAEPVKEEAKVEKAPKVKGDGFSRMKLPSGKPEEAPKKTPKMKIMVGTTGQAKVVEKKDSMDELDAFTGPCYIPPHMDVPPVAPEVLAKITKLKPGVEKDPQLGELPVVILMDDNNTELARLPWQVDISSFSLAQSSMSILVARRLGLLARRLDEDKKDEDTRDWLTGEGLKKFGLSMQKEKPTFGDTSLLLGMGLSALPQALAQKKPEAPKKEEAKPKPEAKKEAPKKGPPGGKGGKGPPGKGPPKGKGKGPKKKGEGGLKVGKKDEGKTKRFFWDPIFEDEIPGTIFMKKPNMVLKLQDVEETFAKVVAKKKTESKKPKIIQLLPDSKRAYNMSIALSKFNNYSYQQLREAIIDLDPKILTIEATETLLNFVPTGEENQVVKEYINSGGDLKLVDKPEQFVAAMLGVPLMKQRLEAHAFALNFREAYSDAYTPLENMADACDAIDDSQNLKIVLFAILELGNALNEGDPQRGGAAGFKPTTLAKLQEIRTTTKPVRTMLQYICDIIWEQQPTALNIYEDLKICDKAQRVDMQGIEGRISNLKAGLTKVKNTLEAAKKGNESTGVMGDRDPLRNIMDEFLIEAEPKIKQLDDFLVQVQNQFLQTCHYSGYPDKDVKKIKPDELFKQVAGFARQVDAIRKQKQEIADRELKRKEAEAKRAGKGLKSTAVKKVGMK

Target ORF information:

RefSeq Version	XM_018779357.1
Organism	Toxoplasma gondii ME49
Definition	Toxoplasma gondii ME49 formin FRM1 (FRM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018779357.1

ORF Insert Sequence:

ATGTCTTGCC CGGCAAGCTG CCCGCGAGGC CGCGGGGGTT CGGCCCCGCT GCCTCCCATG 
CCTCCCTCTG TTAACGACGA TAGAAACGCC GTACTAAATG CATTACCCTC GAGAACGTCT 
TTCTCTGCTC CTCTCTGCTC CGCAACTTCT TGTTCTCTAC CTGAGAGGCC CGTATTCGCC 
AAGTTGACTG GTGGCATGCC TTTCTCTCGA ACAACGGCGC GCATCGAGTG CGCGTCTCCC 
GCGTGTCATG AATCTCTATC GTTACATCGA GTGGCCGAAT CTCAGGTTAA AGCGCAAGCT 
GCCGCCTCTG GGCCTCTGTC CAATTCGTCG TTTCTCATCT TTCTCAATTC TATCCCGACA 
GAACCCCCTC GCTGTTTCCT ACCCCCCCGC CGCGTCGCCT CGCTCTCTGC CGAGGCTGAC 
ACACCCAAGG GTTCCTTCTA TGTCTTCTCC GAGAGTTCTC GGAGTGAGTC TTCGCACAGT 
GAAGGCTGTG TGGAATCCCG CTTGTCTGGG CACACAGCAG CACGCACGAG GTGCCGGAGG 
TCGCGTACCA CTGCTTCGGC TACCGACAGC CGTCGCGATG ACCTTTTTTC GTTTGACCTA 
CCGCAAGAAT GCGGTTCTGC CCCCAGACTT CCTCTTCCGC TTCCTTCCCG TTCGTGTCCC 
GCCTCTCCGT GCCACTCTTT GCTTCCTCCG CTCTCCAATA AACTCACTCC ACCCTGTCGA 
GAGAGCCAAT CTGAAGGGAC TGATCATGGC TCGTCGTCTG CGCGTTGCTG TCAGCCCCGT 
CCAGCGTATC CGCCCCGAAT CACGGATGCG CTGCCTACCG GACAGATTTA TTCTCGTTCT 
CCATCGAGGG TGCGTGAGCG TACTCTTCGA TCAGAGCCAG CAGCACCGGT AAATTATTCA 
GATACTCAAA AGCGTTGCCC TGCCCCGCTC TTCCAATCCA TTCCCGTCCA CAGCATGCCG 
CCACCCCCGC CGCCACCCAA GGGAAAGAAG GGTCCGCTGC CGAAAAAAGC CTCCAACTCG 
CCTCTCCCGC CCAAAGGCAA ATCAACCCCA GACGAAGGGG CCAAAAAAAC GCCACCACCG 
CCGCCTGCAG CCAAAGCGGG AGTTCCTGAA GGCAAAGCGG AGATGCCTTC AGAAAAGGCG 
GAAGTTCCTC AAAAAAAGGC GGGGGTGCCT TCAGAAAAGG CGGACGTTCC TCAAAAAAAG 
GCGGGGGTGC CTAAGGAAAA GGCGGGAATT CCTCAAGAAA AGGCAGGAAT TTCTAAAGGA 
GAATCGGGGA GTCCTGAAGG AAAAACGGGT CCTCTTCCAG GAAAAGCAGG ACCTCCCAAA 
GGAAAGCTGC CGCCTGGTAA AGCGCCCCCT GCAGAAGGGT CTGGTCCTCC GGCAAAGAGC 
GATTTGAAGG CTTCACCTGC AGGCGCCCCG CCCTCTTCGC TTCCTTCCAA GAAGGCTGTC 
GCGTCGGCTG GGAAACCCCT GGGAGGCAAG ACGCCTCCAG CGCCGAGCGT GGCTCCGAAC 
CCACCTCTCG GCAGTTCCGG CCCGCAAGAG TCTGGCTCTT CGACGTACTA CAGAGACACT 
CTGCGAGCAG CTGCGGGCAC GGCGGGCGGG ATCGAGTGCT CAGCGGGGGA GGGAAGCGGG 
GAAACTGTGC CGCCCCCAAA CGAGGCGTAC GCCGCGAAAG CCGCCATGAT GGTGGAGAGT 
CTCAAACTGG CAGACTACAC GAACGTCCCA GCCGCCCACG CGCACGGAAC TGAGCCGCCT 
GCTCTGCCGT TTGCGCCGGG CGCTGACCGG AACCCAGCGA TTTTGACGAG GGCTTTGAAG 
AAGGAAATGG AGTTGCGTCG CGGGCAACCC ACGCAGACCC CCGAGGAGCG GCGGCTGATG 
CTTGCAGGCA CCTTCAGTCT GCACCAAGCG CGGCTCGGGT TCAAGTTGTC ACCGGAGCAC 
CAGCGCGTTG CCGAAGCCCT GATGCTTCTG CGGCGTTTGG AGGACTTCCG CGGCAGCGTC 
CAGGCCGGCG AAGTCCAGCG GATTCGGTAC TTGCTCAGAG TCGGAGCCAC CGAGGCGTTC 
AGCCAGCAAA GGTACTCGGA CGCCATGCTC CAGGCGCTCC ACTCCTACTT GCTCGCAAAG 
TCGTACTACA CAGCGTACCA AAGCGCGCCT CTCGAGGGCG AAATGGTTCC CGAGTTGCTT 
CTTCTCGTGA AGTGCTGCGG CCTGGCGGGC TGCCCCGACC GTGGACAGGA GTATCTGAAG 
GAGCTCCGGT ACATCGTGGA AAATACTTTG ATTGCAGAGG CCGGAGGCAG CGCGGCGGGA 
AACCGAGGAA GTCTGGTCGG CCCGATCATG CGGGTCGAGA AACACGTCCT CGCTTCAGTC 
GTCAGCAGTC TCGCTGAACT GTGCAGTCTG TACAACCAAC CGGACGACGC CAGGCTCTTC 
TACGAAAAAT ACGTGATTCT CACAAAAGAC GTGTATGGCG CAGATTCTCT GGCGGTGAGC 
GACGCACTGA ACACCGTGGC TCTGTACTTC TTTCGGACTC GACAGTACGG CCAAGCGTTG 
CCGCTGGTGG AACAAGTGTT GGCCATCCGG AAAAAGCACT TGGGTGACTT TGACGCTGCG 
GAACCCCACG CCAGAGTCGC GGACTGTTAC GCAAACATTG GCCTTCTTCA TCGACTTCTC 
GGTCACCCTA CGGAGGCGTT TCCGTGTGTG TTGATCGCTC TGGACATGCG CATGCGAATT 
TACCAGACGC GGGAGACGTC AGGCGTCCAG GACTTGGTCT TGGCTCTGGG GTGTCTGCAG 
CATCAAGCCG GCCACTTTCG GGCGGCTCTC GAGGCGTACA AAGAAGTGTG GACGTTCAGG 
AACAACACTC TGGGTGTCAC GCATCCCGAC ACGGTGAACG TCGAGTTGTT GCTGAGGCAG 
TTGGAGAAAG ACCTGGCAGC GCAGATGCAG GAAGACAAGC AGCGCTTTGT TCAGCGGGAC 
AAGGCATTGC CGTCCGCGTC GGCGTTCGAC CTCACGACTC GCGCAAGCTC ACAGATTTCC 
GTTTCCCGTC AGAGTCACCG GCCCGGGGAC TTTTTCTACG AGGAAGCCGA CGGGGCGGCG 
AGCTCGGACC GCGGTCGCAG GGGAAGTCTG GACTTTGCAG ATGCTTCCGC GACGGTGCGG 
CTGTTGAAGC CGGCAGCGGC GGCGACTCTG GAGGCGTACA GACAGCGCCA CGCTGCGTGC 
GGGTGGACGC AGCCCCTACG AGAGAAGAGC CATTTCCACT CTGTGGCTCG CGTCCGGGCG 
CTCTACGGAG AAGAGTGGCG TTCGCTCATG GTTCCCTCAG CGGCGATTCT GCCCTACGTC 
GACGTCGAGG TCACTGGCCC CGATACGCTG AAGCTGGCGG AGTGCTTTCA GTTCCAGGAA 
GCTCTCGCCG TCGACCGGCC GACCCTCCCG GTGGTGAACG TTCCCCAGAT TGAGAGGGGA 
GAGGCCGTCC TCGAGCAGGG CCATCCTCAC ATGGTGTTGA GTCCGGACGC CGCCGAGCTG 
CTCTCGCAGT GGGGCGTTGC GCTTCCCGAG GTCGACGAGA AGGGCCAGGT CATCAACCGG 
GAGGCGAAGC TCGAGAAGCA CGGGCGTCTT TTCCTGCCAC TGGTCGACCC GAGCACGAAC 
CAGCCGTTCC TGGGATTTTA CGGAGAGCCT GTGATGGTGC CGAATCCGGC GGTCTACCAC 
AGCGTGATTG CGCCTGCCAT TCTCGCCTAT GCGCGCCGGC AAGCGGCTCT GGTGGACCGA 
GCGAAGAACG CCGTCGGGCC TGCGCAGAAG CAACGCTCCG CGATGGTCCA GGGCGCGAAG 
CAAGTGGCGC GCGGCGTCGT CATGGCGAAA CTCGGGGCGA AGGGCAAGGC GGGTGCGAAG 
ACACCTGCGC AGGACGCACC CAAAGGCCTG GAAACGAAGG ACGCCCCCGC CACTGCGGCT 
CCCGCAGCAA AAGAAGGTGG CGCACCCGAG ACAGCAGAAA ACGCTCCTCC GAAAGTGGAG 
GCGGCTGCTG CCGCTCCTGA CAAAGAAGAG TCAGTTGAGA AGAAGGAAGA AGCTTCGCCG 
AGTCTGCCGC GGAAGGTGTC GGAACTTCCT GCGAAGAAGA GTCTCTTGAG GTCGAAGGCG 
AGCCAAGAGA TTCCGAAGGC GCCTCCGCTG AAGGACGACT CTTCGGCACC CGAATCAGGG 
AGTGACAGCG GCGCGAGTGA CCGGAAGCGC TCCCCGTCTC TTTCCACGAA GAAGTCTTTC 
GCGAAGAAGA CGTCTGCGGC CCTGCCGACC CCCGCACCTG AAGGTGCGAC TGCGGACGGG 
AAGCCCAAAT CGCTTCTCGG AAGCGGCGCC AAGAAATTCG ACCCTTCCGC CTACAAAGCA 
AAAGTGAAGG CGGGACCTGC ACCTGGGAAG GCCTTGTCGT TTCGTGCCAC TCCGAAGCAT 
GCAGCCGCGT TGCCGCCTTC AAATCACCTT CGCGAGCCCG CGACCCTGTC CGATCCGGAC 
GCGATTCTCG AAAGAAAACA GAAGTCCCGG ACGAAAGCGC AGACGACCGC GGCCGACTCA 
CACGAGCTCG GGCGCAACGA CATGTTAGAA CGAGAAGAAG AGGGAGACGG AGGAGGAGAA 
GAGGGACTGG CCAAGAGGAA GAAAGTGGTT TTCCAGAAGA ATGTGGAAAA AGGCAGGAAG 
GTCTCTGAGC TTCAACATGA GGCTTCGAGG TCAGCGGACG GGCGCGTAGG GAAGAACCAG 
CAGGGACCGG CCGCAGGGAG ACACGGTTTG AAAGCGAAAA AATTGAACGC TGAGACAGAA 
CGAAATTCTG AGTCAGAAGA GAAGAGTTCT TGCGGTTTCG CAGCACCGCA GTCAAGCTGT 
ACACGAAAAC GCGAGGGTTC CCCAGAAACG CGTTGCATCA ACGTCGAGTC CGTTCCCCAA 
CCCCAGGAGG GTCCAGACGG GTCGCGCGAC ACAACACTGG CGTCTCTTTC CCCTCCAGAC 
TCTTCAAGTT CTTTGAGATG TAAAAAAGCG TCTTCGAAGC TCGAGCTCTC TGTCGAATGC 
TCTGCTTCCC GCGACGAACC GTCCGAGGCG GAAGACCGCC GAGAAGAGAA CAGACGAGAG 
GAGAGAAGAG AGAAGACGAG ACGCGATTTC AAGGCGCTTC TGCTGAAGAA GCAACGGGAG 
CACATGAGCG AGACTTTGAT GCGACTGCAG AAAGAGACCG ACAGGGAGGC TTCGGAGTCT 
GCGAGTGAAT GCAAAATTTT TTTGAAAATG CAGCGCTCCC TCGAACGCAA AGACGAAGTG 
GGTCTCTTTT TTCCCCGCGC CTCTGGCAAC CAGACAACTC CTCCCGCCTT CCAACGAGCG 
TCCTGGCAGG CTGCAGCGCT CGCAGGTCAT CTCCGTTCAG GAGACAGTCT CTGGACGAGA 
GGCGAGGAGA CAGCTGAAGC AGACATTATG GGACGCGGGG ATGAGAGTGC TCTCTTGACA 
AGACGAGTTG AAGAAGAGAG GAAACGCGTT AGGGAGAGAC TGTTGGGATG CGACACTCGC 
GCTTCTCACA GGGAGACGAG TCTAGGGGCC GACAGCGGAC TCTTTGCCCT AGATCGAGAA 
GCACAAAGAC CGGCGGAAAA CAGGGAGTTT CCAGAACCCC ACTGCGTTTC TTCGTCACCT 
CGAAAAGCGA TTGATCAGGT GCCGGACTTG AACTGTAGAG CCGCCTTGGA TCCCGAGAGA 
AAAAGCTTTT CGCCGGACTC TGACCGAGAA ACGTCTATTG CGCTCTGCCG CCGTCGAGTG 
TCTCCCTCGG GCCCTGTCTC CTCTCCAGAC TCGCGATGGC GAGGGGACCG GAGGCGAAAC 
GCTTCATCGC GGCCGACTCT TGATTGGTGT CTTTCTCAGC AAGGAAACGC GACAGAGTCT 
TCGTCTCCAC ACAGAGACAC AACCTCCAGA GCGCCAGATT CCCCCCATGT CAAAGCCGCT 
TCCAAAGAGT CAAACTTCCT CTCCATGCCC TTCTTGGCTG CGGCCGCGCC CCCGCCCCCG 
GGGCTCTTTT CGCCCTGGGC GTCTCCGGTG AACTCGGACG GAAATGGGGA CACTGAGGGG 
ACACTGTGGG ACGAGGGGAT CGAGGCAGGT GGGTCCAGTT TGACGAGAAG GACGCGCTCT 
TCGGAACGCT CCCTGGAAAA GAGATCTGTT CGACAGAAAG CTCCTTCACA GGTTTCGGAG 
GCGCGCGGAA CATTGATGTC TTCGTTGAAC GCCGGACTGG AGGCGAAGAA GACGCGGGCC 
GACGAACGCA GAGACATCGG GACCGTGTTC TGCCTTTCGG ACTCGCGAGA CACAGAAGTG 
AAACCCGGAG AAGAGACACT GTTCCAGAGT GCCGCTGGCG AGTCGTGGGG TGTCGACCGG 
AGATACGTCG AGGCTGAGAG AGAGGCTGAG CAGAGGCGGA GACGAAGGAC AGGAGGCTGG 
GGGAGACACG AGGGAACTGC GAATCCGGGA GGTAGAGGAA GGACTGGATC TCGAGGAGAA 
GCGAGCACCT GGAGGAGGGA GAGCACGAGC TCGGCGACCG AGGCTGAGAT AAGGAGAGAA 
CGCGTTGAGC AGTCGCGTCC CGACAGTGGA AGAAGGCGTG ACACGAGAGA AAGAAAACGA 
TCTGTTGCGC AGGGCGCTGT GAATCGGGTT TCGAGAGCGA CGGGGCGAAC TGAAGGAGTC 
GCCGTCCTCG GTCAGGAGAG GCGGAGATGC GTGGACGCAC GCCGAAGAAA CGAAGCGGAA 
GACAGAGAAG GCGAGGATCT TGGAGAAGCG GAGCTCTCTG TTTCCTGCTT TGATTCTGAC 
GGAGACCAGG AGAAGGACTT TCGACGTGAA ACGTCGAGCG AAGAAGACAA CGAAGAAGAC 
GGTGAGGAAG CAGAGTTGTT TTCAACAAAC GAGGTAGAGA CCGACACGCT GCGCTCTGGG 
AGAAGGAAAC AACATTCGGT CTCCTCTCGA AGTGCTTCTC CTTCGCCATG GCGCGTTGGG 
GCAGACAGAA CTCGGATGAA AACGAACTCG CAAGCGTCCG CTGCGCTTTC GGGGATGCGT 
ACATCTCGTT TTCTTCAACC TCCCGCCTTC GATTCTTCTG CGTCGCGGAG CTCTTCTGCG 
TCGCCTTCTC GACGTCGCCG ATTGGAGACT TTGGAGACTT TGGATCTGCA GAAACGCACG 
AGAAAACAGC GAGTGCGAGT AGGAAGCCGA GAGTCCCGAG AAGGAAACGC GCAAAGGAGT 
CCCGGAAACC GACGGAGAGA CAAAAAAGGA CAGACTGAAG GGAGGAGAGG AGACTGGGGA 
AGAGCCTACT TGGAGAGGTT GGTGGTCGGA CAGGAAACAG AGACCGATTC AGAGATCGCT 
ACTCTGGATG AGGAATTGGA TAGAACGGAG TTACCTCGAG TCGTCTCTCC TCAGACCTTC 
CACGAAGCCC CAAATCCGTT TTCTGACCAA GACACAGAGA CAGATTGCTT GTTCTCCACT 
CCTCGACTTC CTGTTTCAAA TGCGGATCCG CAAGCCTCAA AAACGCACTC TGAGGCCGAT 
GAATATGTTG TTTCGTTTGC TTCTCAATCG TCTGCCGCTC GTTCTTCCTC CTGTTATCCT 
TCTCGTTCTC CCTCTTGTTC TGCTTTGTCT CTCTCTTTTG CATGCTCGGA CTCCACTGTA 
GGCGCCGAGA GCGCCACGGG GAGAGAGCGC ACTGTTCATT TGCTTCGCCC GACTTCCTCC 
AGTGTCTCTT CTATTTCGCC CACCTCTTCC GACTGCGGTA GACTCGACAG CGAACTCGAC 
GCACTTCCAG GACTCGGAAA ATGCACTTTG TTTTTCTCGG CTCCTGAGGC GGTGCATGCG 
AGGCCCCTGG CGTCGCCGAG CTTCCGGCAG CGGCTCTTCA GCCGCAGAGA CAGTGGGGCA 
GAGAGGGCCA GCTGCGGAGA CGCGTTCGAT AGTTGGAGCC CAACTAGCGC CGGTGCGGGT 
TCGCGGGTTG TGGAGAGCTG CGGCAGCAAC GTGTCGAGCC CGTCCAGGGG GAGGCTGCGG 
AGTCGCGTGA GCCTTGGCGG ATTGAAGGCC GCTTTTTCGA GGCATTCAGA AAATTCCTCG 
AACGAAAGAC TCTGCTCTCC AATGCACCAA GAAACCTCGA GGAAGGGTCC TCTCGCCGTG 
GCGAGGCGTC TCTCTGGGGT GGCCTCGCGG CTGGTGCATT CGAGGTCGAG CCTTCAAGAA 
CTCGGGGAAG CCGAAGACGA TTCGAGAAAG AGATCCAGCG AGGGAGATTC TCAGGAGATT 
CCCAGGGATT CCGTTGGCGT GACCGCACTC ACTGGAGACA GCGAGACTCC GAACGGCTGG 
GCAGGTGTGG GGGGGCTGGA AGTATCGGCG GGGGAAGACA GCAGAGAGCT CAGAGAATGC 
GAAGAAAAGG AACGAGAGAG AACGCCGCTC GGAGGGTCGC TCCGGAGATT CAGCTTCTCC 
AGCGACCTGA GAAAAGATCC GAGACGCCCA CGACGGACGC TGTCAGATGC AACGGCGACG 
TGCGAAGGGG ATGGGAAGAT GCGCCGCCTT GTCGCGCTTC TTCGCCGGAA AAATAGACGC 
GTAAAAAAAG AAGGAGAACA AGAAAGCGAG AGAGAACGGA GAGACGAAGG AGAACAAGAA 
AGCGAGAGGG AACGGAGGGA CGAAGGAGAA CACGAAGGAG AACAAGAAAG CGAGAGAGAA 
CGAAGAGACG AAAGAGAACA AGGAGGAGAA CAAGAAAGCG AGAATGAACA GGAAGCGGAA 
CATGAAAGCG AGAGAGAACG AAGAGACGAA AGAGAACAAG GAGGAGAACA AGAAAGCGAG 
AGAGAACGAA GAGACGAAAG AGAACAAGGA GGAGAACAAG AAAGCGAGAG AGAACGAAGA 
GACGAAAGAG AACAAGGAGG AGAACAAGAA AGCGAGAATG AACAGGAAGC GGAACAAGAG 
AACGAGAGAG AACAAGCGGT AGGAGAACAA GGGCAGGAGA GAGAAGGAGA GGGAGAGCAA 
AGAAGCGAAT GCGAACAAGG AGGAAGCGTG ATAAATGTTT GGAGACAGGC AGAGAAGGAA 
GAATCCTCAG GAAAGCAACT GAGTAAAGGT GAAGAGTCGA GAGAAGGAGA GAAGGGAGGC 
GTGAGAGCAG CCGACAACCA GGCGTCGGGT GCGACGCAGG GCGAAGAACA AGAGCAGAGT 
GGCTCGTCTC GTTTCTCGCA GCTCCTCGAA AAGTGGGCGA GAATGTCCGG CGGCGCGACA 
CCGAAACAAC GGATCGAGAT GATTTCAGAC AAATCCGAAC CCGGTGGAAG ATGGCGGAAG 
GACATGGGGA TTCTGAAAGA CAGTGAAGAG GAGAGTTTGA TAGGAAAGGA AGGGATTTCA 
CAGCGGCTGA CGGATGCTGA CACTGGAACT GAGAAGACGA GAGAAGCCCA GCGAGAGGAT 
GCCTGGGTCG TGACAGAAAG TGCCAGAGAC AGACGGCGAG CTTTGGGTAC GACAGTGTCT 
CTGGAGGAGG TCTCCTTGTG TGCTGTGGGG AGCTCAGAGA GTCATCTTGA AAAGCTCGGG 
AGCGGAGGTG ACGAGAACGA CTGCGGCAGA GACGCGAGTG AGGAGACCAA GCAAGGGCAT 
TGTTGCGAAG ACAGAAGAAG TCTCAAAAGA GAAAAAGAGG TGTCGGATTG GGGTGTACAG 
ACATCCCATG AGAGAGAGAA GCAGGTGTCT GCACGTGGTG TAGAGACACC AGAGACAAAA 
GAGGAATTGC CCGCTTCTCC TCTCTCTGGC GACTCTTCGT CTTCGCCTTT GGCCGGCAGT 
GCATCCAACG TCTTCCCCGC GCGCAAGCGC TTGTTTTCTT CCCGTCGAAT GCTGCGACTT 
CCGATTCCCT CGAGGAAACT CAGGAAAGCA GACAAGCCTG GAAGTCCCTC AGACGCGCAG 
AACTCTGCGG TGTCTCCCTC GCCCTCGTTT CAGCTCTCTC GGTCAGCGGA GGCCGCGACA 
GGCGGACCCC AAAACGTTGA CGAAAGAGAA GTGGCGGTGC TCTCGGGGGC GTTGGAGGTG 
CAGACCGAAG GTTGGGGTGT ACAGACAGCT CCGGAGACGC CTGGGAATTC GACACACTGG 
GCGAGTCCAA GTTTTCATTC TCTTTTTTCT CGTCGGTTTT CGCTCCGTGA TCGACTGAGG 
CGAAGAAGCT CCGCCAGCAG CATGACCGTG TTGGACATGG AGGAGAACAA GAGTCTGGAC 
GCACTTTCAG TAGATATGCC TGCAACGGAG CCCCAGCGAC CACTGGCGAG CGTTTCCGAC 
TCGCTGGTTT TTTCGGAAGG CTTGTCTTTC TCTTCCTGTG TAGACCCGGT TTCGGACTTC 
GGTGACGACA ACGAGAAGGA TGGAGTTACG CAGGAAACCC GTTCCTCTTG TTCTGAAGTG 
CCATGTGCAG GCGCGAGAGC AGGTGGGCGA GAGTCCCCGA CAAGCAGGAG ACATTCCTTC 
GTGTTTCGGA CGACCCAGCA AAAATACCGG ACAGAAGGCA GTGCAGATAA CAGAGGAAGG 
AGAAGTTTGA AGGTCCTGCG GCGTTCGAGT TTCTCGCGGG CGTTCCGGGG CTTGGTCAAA 
AGTCCTGAGA GACAAATCCT GCGTGACAGA GATCGCGAAG AGGGGAAGGC GCAGGACACC 
GCGAGGAGAT CTGAGAGCGA GAGGGAGGGT GAAAAGGACG ATGAAGAAAA GCAGACAGGG 
AAGAGGGAGG GCAGGAAGTT GCGCCACCTT TCCGCTTCGT TTCTGAGAAT TCCAAGGCCA 
GGGAAACATG AAACCAACAA GGAATCTGTT TGCAAACGCC GCGGGGCTTC CCTTCCCCCT 
TTACCGCCTG AGGCCTCGAG CCCCCACGGC GCCTCACGGC TGCGGGCTGC GGCGGCGAGT 
TTCTCGCGTC TGAGGCGACG CAGTCAATCA AGCGTTAACC GGGGGAAAAA AGAGGCGATA 
GAGATAGAAA AAATGGAAGA GGAAACGAAG GCACAGAATG AAATAAAACA CGAGAGAAAG 
AGTGAACTGA AACAGGAAGA AGTGGATGAA ATGAAACAGG AAAACGTGGA TGAAATAAAA 
CAAGAGAGAA AGGGTGAAGT CAAACAGGAA GAAGTGGATG AAATGAAACA GGAAAACGTG 
GATGAAATAA AACAAGAGAG AAAGAGTGAA CTGAAACAGG AAGAAGTGGA TGAAATGAAA 
CAGGAAAACG TGGATGAAAT AAAACAAGAG AGAAAGAGTG AACTGAAACA GGAAGAAGTG 
GATGAAATGA AACAGGAAAA CGTGGATGAA ATAAAACAAG AGACGCCGGA GACAGTGCCT 
CTCTGGGAAC CGAGCATCAC TTCCCATCTC AGTGACTCTT GCGGCGAAGA TGTCGCCATT 
TTTGCCGTTG AGGCATGCGT GAGAGCCGCA AGAACCTGGC AGAAAGAGGA TGAAACTCTG 
GGCGCTTCCG TCGCAGCGGG GAGAGGAGAA GACGAAGATG AATGCGTGGA TCGAGGAGAC 
GCGCTCACAG GGCAGGACGA CAACGCAGAG GAGACAAGCC AACGATGTAG CTGTTCCGAG 
GACAGTCCGG AGGGAGTCGC TGAACGAGAG TGGATGGGCG GAAGCAGTGA CGGCGCAGAA 
ATCCACTTGT TGCCTGAAGC AGACACCTCA GATGAAGAGG ATGAAGGAGA AGAGGAGGAA 
GAAAGAGAAG AAGAGAAAGA GGAAGAAGGA GAAGGGGAAG AAGGAGAAGA GGAAGAGGAA 
GAAGGAGAAG AGGAAGAAGA GGAAGAGGAA GAAGAGGAAG AAGGAGAAGA AGAGGACATC 
CACGAGCTGG GTGAGAAAGA ATATAGTTCA GCCGAAGAAG AAACGCGTAT TGGAGAAGCA 
GAATTGAGGC ATGGAGTCGC AATTCTTAAT CCATTTGCGT CTTGCGGGGC GGAGGCGTAT 
GGTGAGGAAA GGCCTAAGAC AGGGACGACG GGGAGTGAAG CCGATGACGA CAGCTGGGCC 
GCAAGTGACT GGAGTGTCGG CGCGAGCAGC AGCGACGAAG AGTCCGAGAG ATCTTGGCAA 
GAGGGACCTT CTTCTGAAGA AGACTGTGAC GAAACAGAAG CTGAGAAGAA CGCAGACAGT 
GAGGTGGAGG AGCTTTATGC AGAAAAGCTT TGCAGAAAGA AAGTGCATGC AGAGAACGAT 
GTCGAAGAGT GCAAGACAGA GGTTAGTAAG GCAGAGGAGG GTTTCCAGAA TTCAATGGCA 
TGTCTGGCAG AGGTGCTTCG GCTTACAGGA GTGCCGTATA GTGAAAGCGA TTTGTCTGTC 
GAGATTGCAG AGTTGAATCG ACAATCGACG CTGCTGATGG TGACTCCGGA TACACAGCGG 
CTTTCCCGTG AGCTTCCGAA GTCTATTGCG TCGCCGCGAA CGTCGGCAGT GCCGCTGGAC 
AAACAGTGCG ATGGAAAAAC GAACCTCACA CACCTTCCCA AGAGCGCACC CTTCCGGAAA 
GCGATGGAAC TCACTCGTGT CGCCTATCGG TCAGGCAAAA CGCCGGCTGC TTGTTCAGTC 
CTCCGGTCTT CGTCTTCTTC AAATCTCGCT TCTTCGGTCT CTTCAAGCCG TTCTTCTTCC 
AAACTCGTAT CTTCTTCTAG ACTGTATGCC ACCCCACGAG AGCCTCGCTC TGAGCCGCGT 
GAGGCTTCTG GTGGGTTCTC GGTGTCGCCG ATTGCGTGCG ACTCTTCTCC GTTTGCTCCA 
CCTGCGGCTC CGCTTGGCGC GTCGGAGGGT GGACGCGAAA GTCTTTCGTG CAGCGACTCT 
TCATCCGTCT TGGTTGGAGA TCGTCCCCCG CGTTTCTCGA CCCAGCAGCT CCACGCGCTC 
CCGCCGAAGG GACCGAAGGG CGGGCCGCCA AAGAAAGGCA AGGCGACGGG CTGGAAGCCT 
CCTCCGCCTC TGCCGCCAGC TGCAGAGGAC GGGACAGACG TGAAGGAAGG ACCGACAGTT 
TTGAAGCAAG GCATTTTCAA GAAAGCCGAG CAGGCGGTGA AGAAGGAGAT CCCGAAGAAG 
GAAGAGGGGT CTCCGAAGGA GGCGGCGAAG GTCGTGGAAA AGAAGGTGCT AGAGAAGAAG 
GCGAGTGTGT CCGCAGCCAA GGGCGAGACG GAGACGAAAG TCGCTGTAAA GAGCGTAGCG 
AAACCGCCGC CTCTGAAGGC TGCGAAGAGC GGCGAGGCAA AGACAGCAGA ACCTGTGAAG 
GAAGAAGCAA AAGTGGAGAA GGCCCCGAAG GTCAAGGGCG ACGGCTTTAG CCGCATGAAG 
CTCCCCTCGG GGAAGCCTGA AGAGGCGCCG AAAAAGACTC CCAAGATGAA GATCATGGTC 
GGAACGACGG GTCAGGCGAA GGTCGTCGAG AAGAAAGACT CGATGGACGA ACTGGATGCT 
TTCACGGGGC CTTGCTACAT CCCCCCGCAC ATGGACGTCC CCCCGGTCGC TCCCGAGGTA 
CTCGCAAAGA TCACCAAGTT GAAGCCTGGG GTAGAGAAGG ACCCGCAGCT GGGGGAGCTT 
CCGGTGGTCA TTCTGATGGA CGACAACAAC ACAGAGCTGG CGAGGCTTCC GTGGCAGGTG 
GACATCTCCT CGTTCTCGCT CGCACAAAGT TCGATGTCCA TCTTGGTGGC TCGGAGACTA 
GGTCTCTTGG CGCGACGCCT CGACGAAGAC AAGAAGGACG AGGACACCCG CGACTGGCTG 
ACGGGCGAGG GCCTGAAGAA GTTCGGCCTG TCGATGCAGA AGGAGAAGCC CACCTTTGGA 
GACACTAGTC TCCTCCTGGG CATGGGTCTC TCGGCGCTGC CTCAGGCCCT CGCACAGAAG 
AAGCCGGAGG CACCCAAGAA GGAGGAGGCG AAGCCAAAGC CAGAGGCGAA GAAGGAGGCG 
CCAAAGAAGG GCCCTCCCGG CGGAAAGGGC GGGAAAGGCC CTCCTGGCAA AGGGCCTCCA 
AAGGGCAAGG GCAAGGGCCC GAAGAAGAAG GGCGAAGGCG GTTTGAAGGT CGGCAAGAAG 
GACGAGGGGA AGACCAAGCG GTTCTTCTGG GACCCGATCT TCGAGGACGA AATCCCCGGG 
ACTATCTTCA TGAAGAAGCC GAACATGGTT CTAAAGCTGC AAGACGTAGA AGAGACCTTC 
GCGAAAGTCG TCGCCAAGAA AAAGACCGAG TCAAAGAAAC CCAAGATCAT CCAACTACTC 
CCAGACTCCA AACGCGCATA CAACATGAGC ATCGCCTTGT CCAAGTTCAA CAACTACTCG 
TACCAACAGC TCCGGGAGGC CATCATCGAC CTTGACCCTA AGATCCTAAC AATCGAAGCG 
ACGGAGACTC TCCTGAACTT CGTGCCCACA GGTGAAGAAA ACCAGGTCGT CAAAGAGTAC 
ATTAACTCTG GTGGAGACTT GAAACTCGTC GACAAACCGG AGCAATTCGT CGCAGCAATG 
CTCGGAGTCC CTCTGATGAA ACAGCGCCTT GAGGCCCACG CTTTCGCCTT GAACTTCCGC 
GAGGCGTACA GCGACGCGTA CACTCCACTG GAAAACATGG CAGATGCATG CGACGCCATC 
GACGATTCCC AGAACCTCAA AATTGTTCTT TTCGCCATCC TCGAGTTGGG TAACGCACTC 
AATGAGGGCG ATCCGCAGCG AGGAGGTGCA GCTGGTTTCA AACCGACAAC GCTTGCCAAG 
CTGCAAGAAA TTCGGACAAC AACGAAACCC GTCAGAACAA TGCTGCAGTA CATTTGCGAT 
ATCATTTGGG AGCAACAGCC GACGGCGCTG AACATCTATG AAGATTTGAA GATCTGCGAC 
AAGGCGCAAC GCGTTGACAT GCAGGGCATT GAGGGCAGAA TTTCCAATCT TAAGGCGGGT 
TTGACGAAGG TGAAGAACAC CCTGGAGGCA GCAAAGAAGG GCAACGAGTC CACTGGCGTC 
ATGGGAGATC GCGATCCTCT TCGGAACATT ATGGACGAAT TTCTTATAGA GGCGGAGCCG 
AAGATCAAGC AGCTTGACGA CTTCCTTGTT CAGGTTCAGA ATCAATTTCT GCAGACGTGT 
CATTACTCTG GTTACCCTGA CAAAGATGTC AAGAAGATTA AGCCAGATGA GCTTTTCAAA 
CAGGTTGCAG GCTTCGCAAG GCAGGTGGAT GCCATTCGCA AGCAGAAGCA AGAGATTGCA 
GATCGCGAAT TGAAACGCAA GGAGGCCGAG GCAAAGAGAG CAGGCAAAGG ACTCAAGTCG 
ACTGCAGTCA AGAAAGTCGG GATGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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FRM1 cDNA ORF clone, Toxoplasma gondii ME49

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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