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Human CD27 Antibody (16G7), MAb, Mouse

Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) binding with Human CD27 Antibody (GenScript, A01964-40; blue curve) or binding with an isotype control antibody (red curve), or binding with PBS solution as a negative control (black curve)
Antibody working concentration: 1 µg/ml, 2.5x10⁵ cells/reaction
The signal was developed with R-PE conjugated anti-mouse IgG

Human CD27 Antibody (16G7), mAb, Mouse

The product is specific for human CD27
A01964
$105.00

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Overview
Specificity The product is specific for human CD27
Host Species Mouse
Immunogen Recombinant CD27 protein
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.01-0.05 µg/ml
Flow Cytometry 1- 5 µg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles.
Purification Protein A affinity column
Isotype Mouse IgG2b
Clonality Monoclonal
Clone ID 16G7
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • Human CD27 Antibody (16G7), MAb, Mouse
    • Human CD27 Antibody (16G7), MAb, Mouse

    Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) binding with Human CD27 Antibody (GenScript, A01964-40; blue curve) or binding with an isotype control antibody (red curve), or binding with PBS solution as a negative control (black curve)
    Antibody working concentration: 1 µg/ml, 2.5x10⁵ cells/reaction
    The signal was developed with R-PE conjugated anti-mouse IgG


Background
Target Background CD27, also known as S152 and T14, is a member of the tumor necrosis factor receptor family. It is expressed on memory B cells, a fraction of plasma cells, naive and memory T cells and NK cells. CD27 binds to ligand CD70, and plays an important role in regulating B-cell activation and immunoglobulin synthesis. It is considered as a useful marker to distinguish certain T and B cell subsets from each other.
Synonyms Mouse monoclonal to Human CD27/S152/T14/TNFRSF7/Tp55

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.