MonoRab™ Anti-Bevacizumab Antibody (46E3)[Biotin], MAb, Rabbit

Standard curve of Bevacizumab Sandwich ELISA. The Bevacizumab Sandwich ELISA assay is developed by using MonoRabᵀᴹ Anti-Bevacizumab Antibody (30E1), mAb, Rabbit (GenScript, A01895-40) and MonoRabᵀᴹ Anti-Bevacizumab Antibody (46E3) [Biotin], mAb, Rabbit (GenScript, A01896-40) as the capture and detection antibodies, respectively.
The sensitivity of detecting Bevacizumab is up to 20 pg/ml.

MonoRabᵀᴹ Anti-Bevacizumab Antibody (46E3) [Biotin], mAb, Rabbit (GenScript, A01896-40) blocking with Vascular Endothelial Growth Factor 165 (VEGF 165).
Coating antigen: Bevacizumab, 0.3 µg/ml.
Anti-Bevacizumab Antibody (46E3), mAb, Rabbit (GenScript, A01896-40) final concentration: 8 ng/ml.
VEGF 165 dilution start from 20 µg/ml.
IC₅₀= 46.16 ng/ml.

ELISA binding of MonoRabᵀᴹ Anti-Bevacizumab Antibody (46E3) [Biotin], mAb, Rabbit (GenScript, A01896-40) with Bevacizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Bevacizumab, 1 µg/ml.
Anti-Bevacizumab antibody (GenScript, A01896-40) dilution start from 2,000 ng/ml.
EC₅₀= 4.421 ng/ml.

MonoRab™ Anti-Bevacizumab Antibody (46E3)[Biotin], mAb, Rabbit

The product is specific for Bevacizumab. This antibody blocks Bevacizumab binding with VEGF165 (Z02689, VEGF165, Human). The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody GenScript, A01895-40, MonoRabTM Anti-Bevacizumab Antibody (30E1), mAb, Rabbit.

Cat. No.	A01896
Size	40 μg Buy in bulk
Quantity
Price	$198.00


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Overview

Specificity	The product is specific for Bevacizumab. This antibody blocks Bevacizumab binding with VEGF165 (Z02689, VEGF165, Human). The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody GenScript, A01895-40, MonoRabTM Anti-Bevacizumab Antibody (30E1), mAb, Rabbit.
Host Species	Rabbit
Immunogen	Bevacizumab
Conjugate	Biotin

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.01-0.05 µg/ml

Properties

Form	Lyophilized
Storage Buffer	lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions	The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Rabbit IgG
Clonality	Monoclonal
Clone ID	46E3
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples

Background

Target Background	Bevacizumab (Avastin) is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of patients with colon cancer, lung cancer, glioblastoma, renal-cell carcinoma, and age-related macular degeneration. As an angiogenesis inhibitor, Bevacizumab blocks angiogenesis by inhibiting vascular endothelial growth factor A (VEGF-A).
Synonyms	Rabbit Monoclonal to Avastin

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

MonoRab™ Anti-Bevacizumab Antibody (46E3)[Biotin], mAb, Rabbit

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