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Human TIM3 Antibody (TI.B2), MAb, Mouse

ELISA binding of human TIM3 antibody TI.B2 (GenScript, A01834) with Human TIM3 recombinant protein (2365-TM, TIM3 Fc Chimera, Human).
Coating antigen: TIM3-Fc, 0.5 µg/ml.
TIM3 antibody dilution start from 1000 ng/ml, EC50= 6.98 ng/ml

Human TIM3 Antibody (TI.B2), MAb, Mouse

Flow cytometric analysis of CHO-K1/TIM3 stable cell expressing TIM3 (GenScript, M00531, Red) and CHO negative control cell (Black) binding with Human TIM3 Antibody TI.B2 (GenScript, A01834)
Antibody working concentration: 5 µg/ml, 2.5x105 cells/reaction
The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG

Human TIM3 Antibody (TI.B2), mAb, Mouse

The product is specific for human TIM3 (UniProt Accession: Q8TDQ0). This antibody specifically recognizes human TIM3 recombinant protein in ELISA assay and human TIM3-expressing stable cell line in FACS assay.
A01834
$105.00

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Overview
Specificity The product is specific for human TIM3 (UniProt Accession: Q8TDQ0). This antibody specifically recognizes human TIM3 recombinant protein in ELISA assay and human TIM3-expressing stable cell line in FACS assay.
Host Species Mouse
Immunogen Recombinant human TIM3-Fc (2365-TM)
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.01-0.1 µg/ml
Flow Cytometry 2-10 µg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID TI.B2
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • Human TIM3 Antibody (TI.B2), MAb, Mouse
    • Human TIM3 Antibody (TI.B2), MAb, Mouse

    ELISA binding of human TIM3 antibody TI.B2 (GenScript, A01834) with Human TIM3 recombinant protein (2365-TM, TIM3 Fc Chimera, Human).
    Coating antigen: TIM3-Fc, 0.5 µg/ml.
    TIM3 antibody dilution start from 1000 ng/ml, EC50= 6.98 ng/ml

  • Human TIM3 Antibody (TI.B2), MAb, Mouse
    • Human TIM3 Antibody (TI.B2), MAb, Mouse

    Flow cytometric analysis of CHO-K1/TIM3 stable cell expressing TIM3 (GenScript, M00531, Red) and CHO negative control cell (Black) binding with Human TIM3 Antibody TI.B2 (GenScript, A01834)
    Antibody working concentration: 5 µg/ml, 2.5x105 cells/reaction
    The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG


Background
Target Background TIM-3 (T cell immunoglobulin and mucin domain-3), is a member of the TIM family of immune regulating molecules. Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity.GenScript Human TIM3 Antibody (TI.B2), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from mouse immunized with recombinant human TIM3-Fc (2365-TM).
Synonyms Mouse monoclonal to T-Cell Immunoglobulin Mucin Receptor 3/HAVCR2/ CD366/ KIM-3/ TIMD3/ Tim-3/ TIMD-3/ HAVcr-2

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.