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THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

Immunocytochemistry/Immunofluorescence analysis of Strep II tagged protein transfeced HEK293 cells using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (Cat.No. A01732) and Mouse IgG Control (Whole Molecule), Purified (Cat.No. A01007)
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

Western blot analysis of immunoprecipitates from Strep II tagged protein transfected HEK293 cell lysates using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control.
Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 3: Immunoprecipitates of the Non transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).

THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

Western blot analysis of C-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

Western blot analysis of N-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG

THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

Flow cytometric analysis of non-transfected or Strep II fusion gene transfected CHO cells using
THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) (black and blue respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

THE™ NWSHPQFEK Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
A01732
$319.00

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Overview
Specificity THE™ NWSHPQFEK Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
Host Species Mouse
Immunogen NWSHPQFEK peptide conjugated to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot  0.1-0.5 µg/ml
Immunoprecipitation (IP)  1-5 µg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF)  1-3 µg/ml
ELISA  0.001-0.002 µg/ml
Flow Cytometry  1 µg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID 5A9F9

Examples
  • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
    • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

    Immunocytochemistry/Immunofluorescence analysis of Strep II tagged protein transfeced HEK293 cells using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (Cat.No. A01732) and Mouse IgG Control (Whole Molecule), Purified (Cat.No. A01007)
    The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

  • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
    • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

    Western blot analysis of immunoprecipitates from Strep II tagged protein transfected HEK293 cell lysates using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
    Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control.
    Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
    Lane 3: Immunoprecipitates of the Non transfected HEK293 cell lysates with THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).

  • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
    • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

    Western blot analysis of C-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

  • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
    • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

    Western blot analysis of N-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/ml). The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG

  • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
    • THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

    Flow cytometric analysis of non-transfected or Strep II fusion gene transfected CHO cells using
    THETM NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) (black and blue respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.


Background
Target Background Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag II fusion proteins.
Synonyms THE™ NWSHPQFEK Tag Antibody,mAb,Mouse

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.