Catalog Products » Antibodies » THE Elite Antibodies » THE™ HA Tag Antibody, mAb, Mouse
THE™ HA Tag Antibody, MAb, Mouse

Lane 1-3: 2 μl, 0.4 μl, 0.08 μl N-terminal HA-GST fusion protein in E. coli cell lysate
Lane 4-6: 2 μl, 0.4 μl, 0.08 μl C-terminal HA-GST fusion protein in E. coli cell lysate
Lane 7-9: 500 ng, 250 ng, 125 ng Internal HA tagged Fusion protein (Purified) (GenScript, M0101)
Lane 10: E. coli cell lysate

Primary antibody:
A. 1 μg/ml THETM Anti-HA-Tag Monoclonal Antibody (Mouse) (GenScript, A01244)
B. 1 μg/ml Mouse Anti-HA Monoclonal Antibody (Commerical product, clone 12CA5)

Secondary antibody:
IRDye800 Conjugated affinity Purified Goat Anti-Mouse IgG

THE™ HA Tag Antibody, MAb, Mouse

293 cells transfected with N-terminal HA tag protein
Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

THE™ HA Tag Antibody, MAb, Mouse

293 cells transfected with C-terminal HA tag protein
Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-mouse IgG (Rockland, 610-102-121)

THE™ HA Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected or HA fusion gene transfected CHO cells using
THETM HA Tag Antibody, mAb, Mouse (GenScript, A01244) (black and pink respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE™ HA Tag Antibody, mAb, Mouse

THE™ HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins.
A01244
$287.00

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Overview
Specificity THE™ HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins.
Host Species Mouse
Immunogen A KLH coupled synthetic peptide (YPYDVPDYA)
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Immunoprecipitation (IP) 1 µg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF) 1 µg/ml
Flow Cytometry 1 µg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1
Clonality Monoclonal
Clone ID 5E11D8
Note GenScript can offer this product per customer's reuqest including product size, buffer components,etc.

Examples
  • THE™ HA Tag Antibody, MAb, Mouse
    • THE™ HA Tag Antibody, MAb, Mouse

    Lane 1-3: 2 μl, 0.4 μl, 0.08 μl N-terminal HA-GST fusion protein in E. coli cell lysate
    Lane 4-6: 2 μl, 0.4 μl, 0.08 μl C-terminal HA-GST fusion protein in E. coli cell lysate
    Lane 7-9: 500 ng, 250 ng, 125 ng Internal HA tagged Fusion protein (Purified) (GenScript, M0101)
    Lane 10: E. coli cell lysate

    Primary antibody:
    A. 1 μg/ml THETM Anti-HA-Tag Monoclonal Antibody (Mouse) (GenScript, A01244)
    B. 1 μg/ml Mouse Anti-HA Monoclonal Antibody (Commerical product, clone 12CA5)

    Secondary antibody:
    IRDye800 Conjugated affinity Purified Goat Anti-Mouse IgG

  • THE™ HA Tag Antibody, MAb, Mouse
    • THE™ HA Tag Antibody, MAb, Mouse

    293 cells transfected with N-terminal HA tag protein
    Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
    Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

  • THE™ HA Tag Antibody, MAb, Mouse
    • THE™ HA Tag Antibody, MAb, Mouse

    293 cells transfected with C-terminal HA tag protein
    Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
    Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-mouse IgG (Rockland, 610-102-121)

  • THE™ HA Tag Antibody, MAb, Mouse
    • THE™ HA Tag Antibody, MAb, Mouse

    Flow cytometric analysis of non-transfected or HA fusion gene transfected CHO cells using
    THETM HA Tag Antibody, mAb, Mouse (GenScript, A01244) (black and pink respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.


Background
Target Background GenScript THE™ HA Tag Antibody, mAb, Mouse (A01244) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our THE™ elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. THE™ HA Tag Antibody, mAb, Mouse (GenScript, A01244) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.
Synonyms Mouse Anti-HA-Tag Monoclonal Antibody;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.