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α-Actin-1 Antibody, PAb, Rabbit

Western blot analysis of tissue and cell lysates using α-Actin-1 Antibody, pAb, Rabbit (GenScript, A00885, 1 µg/ml)
The signal was developed with with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 42 kD
Observed Size: 42 kD

α-Actin-1 Antibody, PAb, Rabbit

Western blot analysis of tissue and cell lysates using α-Actin-1 Antibody, pAb, Rabbit (GenScript, A00885, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 42 kD
Observed Size: 42 kD

α-Actin-1 Antibody, pAb, Rabbit

GenScript Rabbit Anti-Alpha-Actin-1 Polyclonal Antibody reacts with human, mouse, rat, cow, pig, rabbit, and some fish species.It has not yet been tested with other species.
A00885
$105.00

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Overview
Specificity GenScript Rabbit Anti-Alpha-Actin-1 Polyclonal Antibody reacts with human, mouse, rat, cow, pig, rabbit, and some fish species.It has not yet been tested with other species.
Host Species Rabbit
Immunogen Synthetic peptide GQVITIGNERFR conjugated to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000

Properties
Form Liquid
Storage Buffer 1×PBS, pH 7.4, containing 0.02% sodium azide
Concentration 0.5 mg/ml
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml, if the antibody is in lyophilized form.
Storage Instructions The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

Examples
  • α-Actin-1 Antibody, PAb, Rabbit
    • α-Actin-1 Antibody, PAb, Rabbit

    Western blot analysis of tissue and cell lysates using α-Actin-1 Antibody, pAb, Rabbit (GenScript, A00885, 1 µg/ml)
    The signal was developed with with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
    Predicted Size: 42 kD
    Observed Size: 42 kD

  • α-Actin-1 Antibody, PAb, Rabbit
    • α-Actin-1 Antibody, PAb, Rabbit

    Western blot analysis of tissue and cell lysates using α-Actin-1 Antibody, pAb, Rabbit (GenScript, A00885, 1 µg/ml)
    The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 42 kD
    Observed Size: 42 kD


Background
Target Background The two major cytoskeletal proteins implicated in cell motility are actin and myosin.Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis.GenScript Rabbit Anti-Alpha-Actin-1 Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide conjugated to KLH.
Synonyms Rabbit Anti-α-Actin-1 pAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.